LOCUS       DAVLYV010000001        78949 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLYV010000001 DAVLYV010000000
VERSION     DAVLYV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 78949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.001"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            561..1013
                     /locus_tag="VYO70_000001"
     CDS             561..1013
                     /locus_tag="VYO70_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1712310.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1032..2369
                     /locus_tag="VYO70_000002"
     CDS             1032..2369
                     /locus_tag="VYO70_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1712311.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2448..3332
                     /locus_tag="VYO70_000003"
     CDS             2448..3332
                     /locus_tag="VYO70_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1712312.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTIFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASVVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3332..4165
                     /locus_tag="VYO70_000004"
     CDS             3332..4165
                     /locus_tag="VYO70_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1712313.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4183..6276
                     /gene="nanB"
                     /locus_tag="VYO70_000005"
     CDS             4183..6276
                     /gene="nanB"
                     /locus_tag="VYO70_000005"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1712314.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFENATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6288..7391
                     /locus_tag="VYO70_000006"
     CDS             6288..7391
                     /locus_tag="VYO70_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1712315.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7565..8263
                     /locus_tag="VYO70_000007"
     CDS             7565..8263
                     /locus_tag="VYO70_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1712316.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8282..9799
                     /locus_tag="VYO70_000008"
     CDS             8282..9799
                     /locus_tag="VYO70_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1712317.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADPAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPIGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9924..11252
                     /locus_tag="VYO70_000009"
     CDS             9924..11252
                     /locus_tag="VYO70_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1712318.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11504..12370
                     /locus_tag="VYO70_000010"
     CDS             11504..12370
                     /locus_tag="VYO70_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1712319.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12386..13225
                     /locus_tag="VYO70_000011"
     CDS             12386..13225
                     /locus_tag="VYO70_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1712320.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTVFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13498..13950
                     /locus_tag="VYO70_000012"
     CDS             13498..13950
                     /locus_tag="VYO70_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1712321.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRRDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKEEAVAFDEASDIG
                     FVHCYEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14047..14391
                     /locus_tag="VYO70_000013"
     CDS             14047..14391
                     /locus_tag="VYO70_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712322.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMSWIIRGIVNGLIGRYIKLGTY
                     AAKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAVAWDA
                     HDKIPNNGRINF"
     gene            14678..15322
                     /locus_tag="VYO70_000014"
     CDS             14678..15322
                     /locus_tag="VYO70_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1712323.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            15491..16408
                     /locus_tag="VYO70_000015"
     CDS             15491..16408
                     /locus_tag="VYO70_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1712324.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16426..17310
                     /locus_tag="VYO70_000016"
     CDS             16426..17310
                     /locus_tag="VYO70_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1712325.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17326..18177)
                     /locus_tag="VYO70_000017"
     CDS             complement(17326..18177)
                     /locus_tag="VYO70_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1712326.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18377..20434
                     /gene="pbp2b"
                     /locus_tag="VYO70_000018"
     CDS             18377..20434
                     /gene="pbp2b"
                     /locus_tag="VYO70_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1712327.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNGRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20445..21041
                     /gene="recR"
                     /locus_tag="VYO70_000019"
     CDS             20445..21041
                     /gene="recR"
                     /locus_tag="VYO70_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1712328.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21217..22260
                     /locus_tag="VYO70_000020"
     CDS             21217..22260
                     /locus_tag="VYO70_000020"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1712329.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22344..23717
                     /locus_tag="VYO70_000021"
     CDS             22344..23717
                     /locus_tag="VYO70_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1712330.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTVIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLFLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAVNGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENEAK"
     gene            23704..24315
                     /locus_tag="VYO70_000022"
     CDS             23704..24315
                     /locus_tag="VYO70_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1712331.1"
                     /translation="MKPSDFVKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDVEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24406..25110
                     /locus_tag="VYO70_000023"
     CDS             24406..25110
                     /locus_tag="VYO70_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1712332.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHVTILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25329..26702
                     /gene="ftsA"
                     /locus_tag="VYO70_000024"
     CDS             25329..26702
                     /gene="ftsA"
                     /locus_tag="VYO70_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1712333.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26719..27978
                     /gene="ftsZ"
                     /locus_tag="VYO70_000025"
     CDS             26719..27978
                     /gene="ftsZ"
                     /locus_tag="VYO70_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1712334.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27983..28654
                     /locus_tag="VYO70_000026"
     CDS             27983..28654
                     /locus_tag="VYO70_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1712335.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKAAQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28664..29203
                     /locus_tag="VYO70_000027"
     CDS             28664..29203
                     /locus_tag="VYO70_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1712336.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTSVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29203..29466
                     /locus_tag="VYO70_000028"
     CDS             29203..29466
                     /locus_tag="VYO70_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1712337.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29463..30248
                     /locus_tag="VYO70_000029"
     CDS             29463..30248
                     /locus_tag="VYO70_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1712338.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30257..31045
                     /gene="divIVA"
                     /locus_tag="VYO70_000030"
     CDS             30257..31045
                     /gene="divIVA"
                     /locus_tag="VYO70_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1712339.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31393..34185
                     /locus_tag="VYO70_000031"
     CDS             31393..34185
                     /locus_tag="VYO70_000031"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1712340.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNAPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GILDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWADVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANGLEVAVTVDERGIMMANAGPEFEGQFYDKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFTLDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEAQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SDLTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            34742..35104
                     /locus_tag="VYO70_000032"
     CDS             34742..35104
                     /locus_tag="VYO70_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712341.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNTLKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            35640..36332
                     /locus_tag="VYO70_000033"
     CDS             35640..36332
                     /locus_tag="VYO70_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1712342.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            36385..38796
                     /locus_tag="VYO70_000034"
     CDS             36385..38796
                     /locus_tag="VYO70_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1712343.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASNGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRYCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            39046..39747
                     /locus_tag="VYO70_000035"
     CDS             39046..39747
                     /locus_tag="VYO70_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712344.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            39749..42457
                     /locus_tag="VYO70_000036"
     CDS             39749..42457
                     /locus_tag="VYO70_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1712345.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEAKLKQAEQNLAQEN
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTIKVLGFHNNEVTLYIYRETIVLSLVGIVLGLIAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(42553..43044)
                     /gene="tpx"
                     /locus_tag="VYO70_000037"
     CDS             complement(42553..43044)
                     /gene="tpx"
                     /locus_tag="VYO70_000037"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1712346.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGHDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(43167..44096)
                     /gene="psaA"
                     /locus_tag="VYO70_000038"
     CDS             complement(43167..44096)
                     /gene="psaA"
                     /locus_tag="VYO70_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1712347.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(44128..44976)
                     /locus_tag="VYO70_000039"
     CDS             complement(44128..44976)
                     /locus_tag="VYO70_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1712348.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(44973..45695)
                     /locus_tag="VYO70_000040"
     CDS             complement(44973..45695)
                     /locus_tag="VYO70_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1712349.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            45986..47878
                     /gene="pepO"
                     /locus_tag="VYO70_000041"
     CDS             45986..47878
                     /gene="pepO"
                     /locus_tag="VYO70_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1712350.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEERSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(48001..48630)
                     /locus_tag="VYO70_000042"
     CDS             complement(48001..48630)
                     /locus_tag="VYO70_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1712351.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAYLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            49587..51809
                     /locus_tag="VYO70_000043"
     CDS             49587..51809
                     /locus_tag="VYO70_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1712352.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            51839..52282
                     /locus_tag="VYO70_000044"
     CDS             51839..52282
                     /locus_tag="VYO70_000044"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1712353.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(52519..52735)
                     /locus_tag="VYO70_000045"
                     /pseudo
     CDS             complement(52519..52735)
                     /locus_tag="VYO70_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(52899..53549)
                     /locus_tag="VYO70_000046"
     CDS             complement(52899..53549)
                     /locus_tag="VYO70_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1712354.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            53661..54503
                     /locus_tag="VYO70_000047"
     CDS             53661..54503
                     /locus_tag="VYO70_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1712355.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            54579..55016
                     /locus_tag="VYO70_000048"
     CDS             54579..55016
                     /locus_tag="VYO70_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1712356.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRN
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            55084..55335
                     /locus_tag="VYO70_000049"
     CDS             55084..55335
                     /locus_tag="VYO70_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712357.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFTFVLLPFLLFLSGIFLIYNGVILLK"
     gene            complement(55477..56550)
                     /locus_tag="VYO70_000050"
     CDS             complement(55477..56550)
                     /locus_tag="VYO70_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1712358.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLELAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            56706..57383
                     /locus_tag="VYO70_000051"
     CDS             56706..57383
                     /locus_tag="VYO70_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1712359.1"
                     /translation="MHKILLVEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            57376..58350
                     /locus_tag="VYO70_000052"
     CDS             57376..58350
                     /locus_tag="VYO70_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1712360.1"
                     /translation="MLDWKQFFLAYLRSRSRLFVYLLSLAFLVLLFQFLFASLGIYFL
                     YFFLLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(58399..60342)
                     /gene="thrS"
                     /locus_tag="VYO70_000053"
     CDS             complement(58399..60342)
                     /gene="thrS"
                     /locus_tag="VYO70_000053"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1712361.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(60400..60576)
                     /locus_tag="VYO70_000054"
     CDS             complement(60400..60576)
                     /locus_tag="VYO70_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1712362.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMKNF
                     LSDKEKQLNVRTRK"
     gene            complement(60583..60723)
                     /locus_tag="VYO70_000055"
     CDS             complement(60583..60723)
                     /locus_tag="VYO70_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HEV1712363.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(61154..61354)
                     /locus_tag="VYO70_000056"
     CDS             complement(61154..61354)
                     /locus_tag="VYO70_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712364.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIISLLYSYRFRFSL"
     gene            61593..62222
                     /locus_tag="VYO70_000057"
     CDS             61593..62222
                     /locus_tag="VYO70_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1712365.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNGKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            62363..62632
                     /gene="rpsO"
                     /locus_tag="VYO70_000058"
     CDS             62363..62632
                     /gene="rpsO"
                     /locus_tag="VYO70_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1712366.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            63446..64060
                     /locus_tag="VYO70_000059"
     CDS             63446..64060
                     /locus_tag="VYO70_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1712367.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(64352..65101)
                     /locus_tag="VYO70_000060"
     CDS             complement(64352..65101)
                     /locus_tag="VYO70_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1712368.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTNKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            65234..67570
                     /locus_tag="VYO70_000061"
     CDS             65234..67570
                     /locus_tag="VYO70_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1712369.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRRSMLRALPSALMVVFGVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFSRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQAKK"
     gene            67672..68349
                     /locus_tag="VYO70_000062"
     CDS             67672..68349
                     /locus_tag="VYO70_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1712370.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGCLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQNHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            68336..69133
                     /locus_tag="VYO70_000063"
     CDS             68336..69133
                     /locus_tag="VYO70_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1712371.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            69150..70253
                     /locus_tag="VYO70_000064"
     CDS             69150..70253
                     /locus_tag="VYO70_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1712372.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTLLSSGYQIGEEVFDQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLAQDMEDYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDEPLLQQME
                     EAALPHYPVLARATSRAERVGIRAYTSDFSPFFGQVPELAGVYVASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
     gene            70260..70406
                     /locus_tag="VYO70_000065"
     CDS             70260..70406
                     /locus_tag="VYO70_000065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712373.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVYRTDEVSNHTHGKATLTWFELD
                     FRRV"
     gene            70509..71528
                     /gene="galE"
                     /locus_tag="VYO70_000066"
     CDS             70509..71528
                     /gene="galE"
                     /locus_tag="VYO70_000066"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1712374.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTVTLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            71530..72495
                     /locus_tag="VYO70_000067"
     CDS             71530..72495
                     /locus_tag="VYO70_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1712375.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTHYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKNKVKRL"
     gene            complement(72546..72752)
                     /locus_tag="VYO70_000068"
     CDS             complement(72546..72752)
                     /locus_tag="VYO70_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1712376.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            72804..73280
                     /locus_tag="VYO70_000069"
     CDS             72804..73280
                     /locus_tag="VYO70_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1712377.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            73289..73960
                     /locus_tag="VYO70_000070"
     CDS             73289..73960
                     /locus_tag="VYO70_000070"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1712378.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            74091..74429
                     /locus_tag="VYO70_000071"
     CDS             74091..74429
                     /locus_tag="VYO70_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1712379.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDIVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            74432..75061
                     /locus_tag="VYO70_000072"
     CDS             74432..75061
                     /locus_tag="VYO70_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1712380.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            75301..76452
                     /locus_tag="VYO70_000073"
     CDS             75301..76452
                     /locus_tag="VYO70_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1712381.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKKASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            76765..77514
                     /gene="truA"
                     /locus_tag="VYO70_000074"
     CDS             76765..77514
                     /gene="truA"
                     /locus_tag="VYO70_000074"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1712382.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IALILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            77504..78277
                     /locus_tag="VYO70_000075"
     CDS             77504..78277
                     /locus_tag="VYO70_000075"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1712383.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            78255..78716
                     /locus_tag="VYO70_000076"
     CDS             78255..78716
                     /locus_tag="VYO70_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1712384.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKFPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT        23419 a        14414 c        17707 g        23409 t
ORIGIN      
        1 taacaaaact aatttagaga atgaaatata gagtatttct ctcttaaaag tttttggtga
       61 aacgagatgt agaaaggaga tttagccaaa gagtctatta gtgctagaat aatagattag
      121 aattatttta gaaaaacgaa gtgagcagct tataaattca agtccccaaa tagattcata
      181 ctagtatctt ttgcaaaaaa taaagggcga cttccttcat gaatatcaat ttcatctata
      241 aggaaggtag ctaattgaac taacttattt attctgtttg tcgctagaaa aatcagacct
      301 ccttgtgaag attgaggaga tacttaatga aaatcaaaga agaaactagc aagctagtag
      361 cagattgccc aaaacaccgc tttgaggttg tagataagac tgacgtatat aatccaaggt
      421 gaagcgactg tggtttgaag agattttcaa agagtataga ctagagagta gtgtttttat
      481 gtccttctag tagaaaatgc tagacagaag aatggggaac ttggatagga aaaatagatt
      541 gagaaaggag gttagaagag atgattatta caaaaattag ccgtttagga acttatgtgg
      601 gagtaaatcc acattttgca acattaatag attttctaga aaaaacagga ctagaaaatt
      661 taacagaagg ttcgattgct atcgatggta atcgattgtt tgggaattgc tttacttatc
      721 tagcagatgg tcaagcaggg gatttctttg aaacacacca aaaatatttg gatattcatt
      781 tagttttgga aaacgaagaa gccatggctg ttacatcgcc ggaaaatgta agcgttaccc
      841 aagaatatga tgaagagaaa gatattgaat tatacacagg gaaagtggaa cagttggttc
      901 atttgagagc tggtgaatgc ctcatcactt ttccagaaga tttacatcaa cccaaggttc
      961 gtataaatga tgaacctgtg aaaaaagttg tctttaaagt tgcgatttct taatgtagaa
     1021 agagaagaac gatgaaaaaa atgagaaagt ttttatgtct agctggaatt gcgctagcgg
     1081 ctgttgcctt ggtagcttgt tcaggaaaaa aagaagctac aactagtact gaaccaccaa
     1141 cagaattatc tggtgagatt acaatgtggc actcctttac tcaaggaccc cgtttagaaa
     1201 gtattcaaaa atcagcagat gctttcatgc aaaagcatcc aaaaacgaaa atcaagattg
     1261 aaacattttc ttggaatgac ttctatacta aatggactac aggtttagca aatggaaatg
     1321 tgccagatat cagtacagct cttcctaacc aagtaatgga aatggtcaac tcagacgctt
     1381 tggttccgct aaatgattct atcaagcgta ttggacaaga taaatttaac gaaactgcct
     1441 taaatgaagc aaaaatcgga gatgattact actctgttcc tctttattca catgcacaag
     1501 tcatgtgggt tagaacagat ttgttaaaag aacataatat tgaggttcct aaaacttggg
     1561 atcaactcta tgaagcttct aaaaaattga aagaagctgg agtttatggc ttgtctgttc
     1621 cgtttggaac aaatgactta atggcaacac gtttcttgaa cttctacgta cgtagtggtg
     1681 gaggaagcct cttaacaaaa gatcttaaag cagacttgac aagccaactt gctcaagatg
     1741 gtattaaata ctgggttaaa ttgtataaag aaatctcacc tcaagattct ttgaacttta
     1801 atgtccttca acaagctacc ttgttctatc aaggaaaaac agcatttgac tttaactctg
     1861 gcttccatat cggaggaatt aatgccaaca gtcctcaatt gattgattcg attgatgctt
     1921 atcctattcc aaaaatcaaa gagtctgata aagatcaagg aattgaaacc tcaaacattc
     1981 caatggttgt ttggaaaaat tcaaaacatc cagaagttgc taaagcattc ttagaagcac
     2041 tttataatga agaagactac gttaaattcc ttgattcaac tccagtaggt atgttgccaa
     2101 ctattaaggg gattagcgat tctgcagcct ataaagaaaa tgaaactcgt aagaaattta
     2161 aacatgctga agaagtaatt actgaagctg ttaaaaaagg tactgctatt ggttatgaaa
     2221 atggaccaag tgtacaagct ggtatgttaa ctaaccaaca cattattgaa caaatgttcc
     2281 aagatatcat tacaaatgga acagatccta tgaaagcagc aaaagaagca gaaaaacaat
     2341 taaatgattt atttgaggct gttcagtaga tgtaaaagac tagaaaatag gtgggctagt
     2401 gagctgaaaa gctctagccc aatcttgtaa aagaagggag aaggagaatg gttaaagaac
     2461 gtaatttaac tcgctggata tttgttttgc cagctatgat tatcgtagga ttactctttg
     2521 tttatccgtt tttctcgagt attttttata gctttaccaa taagcatttg attatgccta
     2581 attataaatt tgttggtttg gctaactata aagctgtgct atcagatccc aacttcttta
     2641 atgcgttctt taattcaatt aagtggacca ttttctcatt agttggtcaa gttttagtag
     2701 ggtttgtatt ggctttagct cttcacagag tacgccactt caagaaatta tataggacct
     2761 tattgattgt tccttgggca tttcctacca tcgtcattgc cttttcttgg cagtggattc
     2821 taaacggggt ttatggctac ttacctaatc taatcgtaaa attaggttta atggaacata
     2881 cacctgcatt tttgacagat agtacatggg cattcctatg tttggtgttt atcaacattt
     2941 ggtttggagc accaatgatt atggttaatg tgctttcagc tttgcaaaca gtaccagaag
     3001 aacaatttga ggctgctaag atagatggtg cttcaagttg gcaggtgttc aagtttatcg
     3061 tctttccaca tattaaagtg gttgtaggac ttctagttgt tttgagaact gtatggatct
     3121 ttaataactt tgacattatc tacctcatta ctggtggtgg accagccaat gctacaacga
     3181 cgcttccaat ttttgcttac aacctgggct ggggaactaa attgttgggt cgtgcttcag
     3241 tagttacagt attgctcttt atcttcttgg tggcgatttg ctttatctac tttgctatca
     3301 tcagtaagtg ggaaaaggag ggtagaaaat aatgaagaag aaatccagta tttatttaga
     3361 tattctctca catgtacttt tagttggtgc gaccatcgtt gcaattttcc cattggtatg
     3421 gattatcata tcttctgtca aagggaaagg ggaattaact cagtatccaa cacgattttg
     3481 gcctgaacag tttacattag attatttcac tcatgttatc aacgatttgc acttcattga
     3541 taacattcga aacagtttaa tcattgcctt ggctacaacc ctcattgcga ttattatttc
     3601 tgctatggca gcctatggta ttgttcgatt ctttcctaaa ttgggagcaa tcatgtcgag
     3661 actactcgtc attacctaca ttttcccacc aattttgtta gcaattccct attcaattgc
     3721 cattgctaaa gttgggttaa caaatagttt atttggcttg atgatggttt atctatcttt
     3781 tagtgttcca tatgcagttt ggctcttagt tggatttttc caaacagttc caattggaat
     3841 tgaagaagcg gctagaattg atggtgcaaa taaatttgtt acgttttata aagttgtgct
     3901 accgattgta gcaccaggta ttgtagcaac agctatttat acatttatca atgcttggaa
     3961 tgaattcctg tatgccttga ttttgattaa caatacagga aagatgacag tagcagtagc
     4021 ccttcgttca cttaatggtt cagaaatact agactgggga gatatgatgg cagcgtctgt
     4081 tattgtagtt cttccatcaa ttattttctt ctctatcatc caaaataaga ttgcaagtgg
     4141 attatcagaa ggatctgtga agtagacgaa agaaggaaaa aaatgaataa aagaggtctt
     4201 tattcaaaac taggaatttc cgttgtaggc attagtcttt taatgggagt ccccactttg
     4261 attcatgcga atgaattaaa ctatggtcaa ctgtccatat ctcctatttt tcaaggaggc
     4321 tcatatcaac tgaacaataa gagtatagat atcagccctt tgttattaga taaattgtct
     4381 ggagagagtc agacagtagt aatgaaattt aaagcagata aaccaaactc tcttcaagct
     4441 ttgtttggcc tatctaatag taaagcaggc tttaaaaata attacttttc aattttcatg
     4501 agagattctg gtgagatagg tgtagaaata agagacgccc aaaagggaat aaattattta
     4561 ttttctagac cagcttcatt atggggaaag cataaaggac aggcagttga aaatacacta
     4621 gtatttgtat ctgattctaa agataaaaca tacacaatgt atgttaatgg aatagaagtg
     4681 ttctctgaaa cagttgatac atttttgcca atttcaaata taaatggtat agataaggca
     4741 acactaggag ctgttaatcg tgaaggcaag gaacattacc tcgcaaaagg aagtattgat
     4801 gaaatcagtc tatttaacaa agcaattagt gatcaggaag tttcaaatat tcccttgtca
     4861 aatccatttc agttaatttt ccaatcagga gattctactc aagctaacta ttttagaata
     4921 ccgacactat atacattaag tagtggaaga gttctatcaa gtattgatgc acgttatggt
     4981 gggactcatg attctaaaag taagattaat attgccactt cttatagtga tgataatggg
     5041 aaaacgtgga gtgagccaat ttttgctatg aagtttaatg actatgagga gcagttagtt
     5101 tactggccac gagataataa attaaagaat agtcaaatta gtggaagtgc ttcattcata
     5161 gattcatcca ttgttgaaga taaaaaatct gggaaaacga tattactagc tgatgttatg
     5221 cctgcgggta ttggaaataa taatgcaaat aaagccgact caggttttaa agaaataaat
     5281 ggccattatt atttaaaact aaagaagaat ggagataacg atttccgtta tacagttaga
     5341 gaaaatggtg tcgtttatga tgaaacaact aataaaccta caaattatac tataaatgat
     5401 aagtatgaag ttttggaggg aggaaagtct ttaacagtcg aacaatattc ggttgatttt
     5461 gatagtggct ctttaagaga aaggcataat ggaaaacagg ttcctatgaa tgttttctac
     5521 aaagattcgt tatttaaagt gactcctact aattatatag caatgacaac tagtcagaat
     5581 agaggagaga gttgggaaca atttaagttg ttgcctccgt tcttaggaga aaaacataat
     5641 ggaacttact tgtgtcccgg acaaggttta gcattaaaat caagtaacag attgattttt
     5701 gcaacatata ctagtggaga actaacctat ctcatttctg atgatagtgg tcaaacatgg
     5761 aagaaatcct cagcttcaat tccgtttgaa aatgcaacag cagaagcaca aatggttgaa
     5821 ctgagagatg gtgtgattag aacattcttt agaaccacta caggtaagat agcttatatg
     5881 actagtagag attctggaga aacatggtcg gaagtttcgt atattgatgg aatccaacaa
     5941 acttcatatg gcacacaagt atctgcaatt aaatactctc aattaattga tggaaaagaa
     6001 gcagtcattt tgagtacacc aaattctaga agtggccgta agggaggtca attagttgtc
     6061 ggtttagtca ataaagaaga tgatagtatt gattggaaat accactatga tattgatttg
     6121 ccttcgtatg gttatgccta ttctgcgatt acagaattgc caaatcatca cataggtgta
     6181 ctgtttgaaa aatatgattc gtggtcgaga aatgaattgc atttaagcaa tgtagttcag
     6241 tatatagatt tggaaattaa tgatttaaca aaataaagga gaaaaacatg gttaaatacg
     6301 gtgttgttgg agcagggtat tttggagctg aattggctcg ctacatgcaa aaaaatgatg
     6361 gagcagagat tactcttctc tatgatccag ataatgcaga ggcgattgca gaagaattgg
     6421 gagcaaaagt agcaagttcc ttagatgagt tggtttctag cgatgaagta gactgtgtta
     6481 tcgtcgcaac tccaaataat cttcataagg aaccggttat taaggctgca cagcatggta
     6541 aaaatgtttt ctgtgaaaaa ccaattgcgc tttcttatca agattgtcgc gagatggtag
     6601 atgcgtgtaa agaaaacaat gtaaccttta tggcaggaca tattatgaat ttctttaatg
     6661 gtgttcatca tgcaaaagaa ctcattaatc aaggagttat cggagacgtt ctatattgtc
     6721 atacagctcg taatggttgg gaagaacaac aaccgtcagt atcatggaaa aaaattcgtg
     6781 aaaaatcagg tggtcacttg tatcaccaca tccatgaatt ggattgcgtt caattcctta
     6841 tggggggcat gcctgaaact gtaaccatga caggtggaaa tgtggcccat gaaggtgaac
     6901 atttcggtga tgaagatgat atgatttttg tcaatatgga attttctaat aagcgttttg
     6961 ccttgttaga atggggttca gcttatcgtt ggggtgaaca ttatgtctta atccaaggaa
     7021 gcaaaggtgc catccgctta gacttattca actgtaaagg aactcttaag ctagatgggc
     7081 aagaaagcta tttcttgatt cacgaatcgc aagaagaaga tgatgatcgg actcgtatct
     7141 atcatagtac agagatggat ggagcaattg cttatggtaa accaggtaaa cgtactccat
     7201 tatggctatc atctgtcatt gataaagaaa tgcgctatct gcatgagatt atggaaggag
     7261 ccccagtatc agaagaattt gcaaaacttt tgacaggtga agctgcccta gaagcaattg
     7321 ctactgcaga tgcttgtacc cagtctatgt ttgaagatcg caaagtaaaa ttgtcagaaa
     7381 ttgtaaaata aattttggta ttctcctatt tataggtcga cttgctcctc tgaaagtact
     7441 tttagaggag ctgtttgact ttgctagttt ttgaaactga aatctattat actacaaact
     7501 attgaaagcg ttttaatttt aaggtataat aatctcatag aaataaagaa aaggaggaaa
     7561 gaggatgcca cagattagca aagaagcctt gattgagcaa atcaaagatg gaatcatcgt
     7621 ttcttgtcag gctcttcctc atgaaccgct ttatacagaa gcgggagggg tgattccctt
     7681 gctggtcaaa gcggctgagc aaggtggagc agtcggtatc cgagcaaaca gtgttcgcga
     7741 tatcaaggaa attaaggaag tcactaaact tccaatcatt gggattatca aacgtgatta
     7801 tccacctcag gaacccttca tcacggctac tatgaaagaa gttgatgaat tggcagaact
     7861 ggacatcgag gtgattgctc tggattgtac caagcgtgaa cgctacgatg gtttggaaat
     7921 tcaagagttc attcgtcagg ttaaggagaa atatcctaat cagcttttga tggctgatac
     7981 tagtatcttc gaagaagggc tagcagctgt agaagcagga attgactttg tcggaacaac
     8041 cttatcaggc tacacatcct acagtccaaa agtagacggt ccagattttg aattgattaa
     8101 gaaactctgt gatgccggtg tagatgtcat tgcagaagga aaaattcata caccagaaca
     8161 agccaaacaa atccttgaat atggagtgcg aggcatcgtt gttggtggcg ccattactag
     8221 accaaaagag attacagaac gcttcgttgc tagtcttaaa taagatgtga gagggagttt
     8281 tatgtttaaa gttttacaaa aagttggaaa agcttttatg ttacctatag ctatacttcc
     8341 tgcagcaggt ctacttttgg ggattggtgg tgcactttca aacccaacca cgatagcaac
     8401 ttatccaata ctagacaata gtatttttca atcaatattc caagtaatga gctctgcagg
     8461 agaggttgta ttcagtaatt tgtcactact tctctgtgtg ggattatgta ttggcttagc
     8521 gaaacgagat aaaggaaccg ctgcgttagc aggagtaact ggttacttag ttatgactgc
     8581 aacgatcaaa gctttggtaa aactttttat ggcagaagga tctgcaattg atactggagt
     8641 tattggagca ttagttgtcg gaatagttgc cgtatatttg cacaaccgat ataacaatat
     8701 tcaattacct tccgctttag gattctttgg aggttcacgc ttcgttccta ttgttacatc
     8761 gttctcttct atcttgattg gctttgtctt ctttgttatt tggccacctt tccaacaact
     8821 tcttgtttct acaggtggat atatttctca ggcgggtcca attggaactt ttctatatgg
     8881 atttttaatg agactttctg gagcagtagg cttacatcat ataatttacc ctatgttttg
     8941 gtatactgaa cttggtggtg ttgaaactgt tgcaggacaa acagtggttg gagctcaaaa
     9001 aatatttttt gctcaattag ccgatccggc ccattctgga ttatttacag aaggaacaag
     9061 gttttttgca ggtcgtttct caacaatgat gttcggttta ccggctgcct gtttagcgat
     9121 gtaccatagt gttcctaaaa atcgtcgtaa aaaatacgcg ggtttgtttt ttggagttgc
     9181 tttaacatct tttattaccg gtattacaga accaattgaa tttatgtttc tattcgtcag
     9241 tccggttcta tatgttgttc acgcattcct tgatggtgtt agcttcttta ttgcagacgt
     9301 cttaaatatt tcaataggaa acacattttc aggaggtgta atcgatttca ctttatttgg
     9361 aattttgcag gggaacgcta agacgaattg ggttcttcag attccaattg gacttatttg
     9421 gagtgttttg tattatatta tttttagatg gttcattact caattcaacg ttctaacgcc
     9481 aggacgagga gaagaagtag attctaaaga aatttctgaa tccgcagatt caacttcaaa
     9541 tactgcagat tatttaaaac aggatagcct acaaattatc agagccttgg gtggatcaaa
     9601 taatatagaa gatgtagatg cttgtgtgac acgtttacgt gtagctgtaa aagaagttaa
     9661 tcaagttgat aaagcacttt taaaacaaat tggtgcagtt gatgtcttag aagtgaaggg
     9721 tggcattcaa gcaatatatg gagcaaaagc aatcttatat aaaaatagta ttaatgaaat
     9781 tttaggtgta gatgattaag tacttactga cttaataaaa aacagaggag agtgatggat
     9841 gagtaggatg aaatgaaatc gcatacaaga aataaagaac tcattatcca agttggatac
     9901 gcttattaca taggagaata caaatgaaat ttagaaaatt agcttgtaca gtacttgcgg
     9961 gtgctgcggt tcttggtctt gctgcttgtg gcaattctgg cggaagtaaa gatgctgcca
    10021 aatcaggtgg tgacggtgcc aaaacagaaa tcacttggtg ggcattccca gtatttaccc
    10081 aagaaaaaac tggtgacggt gttggaactt atgaaaaatc aatcatcgaa gcgtttgaaa
    10141 aagcaaaccc agatataaaa gtgaaattgg aaaccatcga cttcaagtca ggtcctgaaa
    10201 aaatcacaac agccatcgaa gcaggaacag ctccagacgt actctttgat gcaccaggac
    10261 gtatcatcca atacggtaaa aacggtaaat tggctgagtt gaacgacctc ttcacagatg
    10321 aatttgttaa agatgtcaac aatgaaaaca tcgtacaagc aagtaaagct ggagacaagg
    10381 cttatatgta tccgattagt tctgccccat tctacatggc aatgaacaag aaaatgttag
    10441 aagatgctgg agtagcaaac cttgtaaaag aaggttggac aactgatgat tttgaaaaag
    10501 tattgaaagc acttaaagac aagggttaca caccaggttc attgttcagt tctggtcaag
    10561 ggggagacca aggaacacgt gcctttatct ctaaccttta tagcggttct gtaacagatg
    10621 aaaaagttag caaatataca actgatgatc ctaaattcgt caaaggtctt gaaaaagcaa
    10681 ctagctggat taaagacaat ttgatcaata atggttcaca atttgacggt ggggcagata
    10741 tccaaaactt tgccaacggt caaacatctt acacaatcct ttgggcacca gctcaaaatg
    10801 gtatccaagc taaactttta gaagcaagta aggtagaagt ggtagaagta ccattcccat
    10861 cagacgaagg taagccagct cttgaatacc ttgtaaacgg atttgcagta ttcaacaata
    10921 aagacgacaa gaaagtcgct gcatctaaga aattcatcca gtttatcgca gatgacaaag
    10981 agtggggacc taaagacgta gttcgtacag gtgctttccc agtccgtact tcatttggaa
    11041 aactttatga agacaaacgc atggaaacaa tcagcggctg gactcaatac tactcaccat
    11101 actacaacac tattgatggt tttgctgaaa tgagaacact ttggttccca atgttgcaat
    11161 ctgtatcaaa tggtgacgaa aaaccagcgg atgctttgaa agccttcact gaaaaagcaa
    11221 acgaaacaat caaaaaagct atgaaacaat agtccttagt tattctataa aaagtagttt
    11281 tttaaagaac ctaagagtgt ataccccctt ttccctctac acagatagtg taagaaaagg
    11341 gggcttttgt ttaaaatgta agaaactgtc acgaaattaa aatgaagttc ttacataagc
    11401 gaatcataaa aaatttcatt ttgattttaa aacagttcaa gaaagtcaaa aaattattct
    11461 atttgaaaga gaggtgccga ctgtgaaagt caataaaatc cgtatgcggg aaacagtgat
    11521 ttcctacgct ttcctagcac cagtattatt cttctttgtc atctttgtgt tggctccgat
    11581 ggtgatgggc ttcattacaa gtttctttaa ctactcaatg actaaatttg agtttgtagg
    11641 cttggataac tatatccgta tgtttaaaga tcctgtcttt acaaaatctc tgattaacac
    11701 agttattttg gttattggat ctgtaccagt tgttgttcta ttctcactct ttgtagcatc
    11761 tcagacctat catcaaaatg tcattgccag atccttctac cgtttcgtct tcttccttcc
    11821 tgttgtaacg ggtagtgttg ccgtgacagt tgtttggaaa tggatttatg acccgctatc
    11881 agggattcta aactttgtcc ttaagtcaag ccacatcatc agccaaaaca tttcttggtt
    11941 gggagataaa aactgggcat tgatggcgat tatgattatt ctcttgacca cttcagttgg
    12001 tcagcccatc atcctttata tcgctgccat ggggaatatt gacaattcac tggttgaagc
    12061 ggcgcgtgtt gatggtgcaa ctgagtttca agttttttgg aagattaaat ggccaagcct
    12121 tcttccaaca actctttata ttgcaatcat cacaacaatt aactcattcc agtgtttcgc
    12181 cttgattcag cttttgacat ctggtggtcc aaactactca acaagtacct tgatgtacta
    12241 cctttacgaa aaagccttcc aattgacaga atacggctat gccaacacaa ttggtgtctt
    12301 cttggcagtc atgattgcta tcgtaagctt tgttcaattt aaagtacttg gaaacgacgt
    12361 agaatactaa agaaaggaga cagctatgca atctacagaa aaaaaaccat taacagcctt
    12421 tactgttatt tcaacaatca ttttgctctt gttgactgtg ctgttcatct ttccattcta
    12481 ctggattttg acaggggcat tcaaatcaca acctgataca attgttattc ctcctcagtg
    12541 gttccctaaa atgccaacca tggaaaactt ccaacaactc atggtgcaga accctgcctt
    12601 gcaatggatg tggaactcag tatttatctc attggtaacc gtgttcttag tttgtgcaac
    12661 ctcatctcta gcaggttatg tattggctaa aaaacgtttc tatggtcaac gcattctatt
    12721 tgctatcttt atcgctgcta tggcgcttcc aaaacaagtt gtccttgtac cattggtacg
    12781 tatcgtcaac ttcatgggaa tccacgatac tctctgggca gttatcttgc ctttgattgg
    12841 atggccattc ggtgtcttcc tcatgaaaca atttagtgaa aatattccta cagagttgct
    12901 tgaatcagct aaaatcgacg gttgtggtga gattcgtacc ttctggagtg tagccttccc
    12961 gattgtgaaa ccagggtttg cagcccttgc aatctttacc ttcatcaata cttggaatga
    13021 ctacttcatg caattggtaa tgttgacttc acgtaacaat ttgaccatct cacttggggt
    13081 tgcgaccatg caggctgaaa tggcaaccaa ctatggtttg attatggcag gagctgccct
    13141 tgctgctgtt ccaatcgtca cagtcttcct tgtcttccaa aaatccttca cacagggtat
    13201 tactatggga gcggtcaaag gataatactc tgcgaaaatc gaatgcaaac tacgtcagct
    13261 tcaccttgcc atacttaagt attgcctgcg gttagcttcc tagtttgttc ttcaattttc
    13321 attgagtata ggaaaatcaa tctatcaaga tacagaagta tattttatag atttagagaa
    13381 tatagaagtt ataagtgtct acaaaatgga gggtatgcag ttactttatg aagttttgtc
    13441 agacacttat caacttaaga atgattttag ttaattatca gaaacgaagg aaagagtatg
    13501 atttttgacg atttgaaaaa catcaccttt tacaaaggga ttcatcccaa tttagacaag
    13561 gctatcgact atctctacca acatcgtaga gattcattcg aattaggaaa gtatgagatt
    13621 gatggagata aagtctttct agttgttcag gaaaatgtcc tcaatcaagt tgagaataat
    13681 caatttgaac accataagaa ctatgcagat ttgcatttgc tgatagaagg gcatgaatat
    13741 tcgagctacg gttcacgtat caaagaagag gcagtagcat tcgacgaagc gagtgacatt
    13801 ggctttgttc attgttatga acactaccca ctcttattgg gttatcacaa ttttgcgatt
    13861 ttcttcccag gtgagccaca tcagccaaat ggttatgcag gcatggaaga aaaggttcga
    13921 aaatatctct ttaaaatctt gattgattaa aaataggatg aattgttttt ttgtaaagct
    13981 ttgataatac tctaccatga aattgatctt tgtgaggtag agaaatgaga ataaaatatt
    14041 taaaaattgg tatcttctaa gtatgctgca agagctagtt tcttagatgg acaggggatt
    14101 acagttgatg agatgtcctg gataattagg ggcattgtga atggattgat tggtagatac
    14161 ataaaattag gtacttatgc agctaagtat ggtattagta tggcgcgctc gatcttaagt
    14221 agggcagctg caactgcggc agcaagagta ggattattga ctaagatttt tggatggatt
    14281 ttacgaatag ctgtgctcgc agctgatgta tatggtaatt ttgccaacaa tattgctgta
    14341 gcttgggatg cacatgataa aatccctaac aatggtcgta taaactttta aaatgcgaga
    14401 atgaaagcac tttgtatttt tttattgaat atgttagctt ggacagtgct tgcaatgata
    14461 attcgtggag ggctagatgg atttaatgag catacttgga gtactatttt aattgcttcg
    14521 ctgtccggga tatatataga ttatatgtcc atagataaaa gtagaaaaaa gtccaaaaga
    14581 aaaaagaaaa aatagatttg ttcatggtag ggacttatga aagttttact gacaaaaaag
    14641 aaaacagttt acaaagaaaa atgatggagg agcaaacatg gcacaaaaag gagtaagcct
    14701 tatcaaggca gcatttgata cagataactt tctcatgcgt tttagtgaga aagtcttgga
    14761 catcgtgaca gccaatcttc tttttgtcgt ctcttgtttg cccatcgtga cgattggagt
    14821 ggctaaaatc agcctctacg agaccatgtt cgaagttaag aagagcagac gggtgcctgt
    14881 ttttaaaatc tatctaagat ctttcaagca aaatctgaaa ctaggtcttc agctgggttt
    14941 aatggagtta ggaattgtgt ttcttaccct ttcagatctc tatcttttct ggggtcaaac
    15001 agctctgccc ttccaattgc tgaaagccat ttgtttaggt attctgattt ttcttactat
    15061 cgtgatgctg gctagttacc ctatcgcggc acgttatgac ctatcttgga aagaaattct
    15121 tcaaaaagga ttgatgttgg ctagttttaa ctttccttgg ttcttcctca tgttagccat
    15181 tcttgtcctc attgtgatgg ttctttatct gtccgccttc agtctactct taggtggctc
    15241 agtcttccta ctttttgggt ttggactatt ggtctttatc cagactggat tgatggagaa
    15301 aattttcgca aaataccaat aggagcttta tttctgaaac tactttcaaa ggctccaaac
    15361 gctattctat aagcgagaaa ctaaaatcta agtgtctata agatattgaa agccttttcg
    15421 gtaaagtgta tactaaagaa gtaaagaaaa atctgcttta gcagaaaata aaaataatag
    15481 gagaaaatct atgtcagatt tgaaaaaata cgaaggtgtc attccagcct tctacgcatg
    15541 ttatgatgat caaggagaag taagcccaga acgtacgcgt gccttggttc aatacttcat
    15601 tgataaaggt gttcaaggtc tttatgtcaa tggttcttct ggtgaatgta tctaccaaag
    15661 cgttgaagat cgcaagttga ttttggaaga agtcatggcg gtagccaaag gtaaattgac
    15721 cattattgcc catgttgctt gcaataatac taaagatagt atggaacttg ctcgccatgc
    15781 tgaaagcttg ggagtagatg ctattgcaac gattccacca atttatttcc gcttgccaga
    15841 atactcagtt gccaaatact ggaacgatat cagttctgca gctccaaaca cagactacgt
    15901 gatttacaac attcctcaat tggcaggggt tgctttgact ccaagccttt acacagaaat
    15961 gttgaaaaat cctcgtgtta tcggtgtgaa gaactcttct atgccagttc aagatatcca
    16021 aacctttgtc agccttggtg gagaagacca tatcgtcttt aatggtcctg atgagcagtt
    16081 cctaggagga cgcctcatgg gggctagggc tggtatcggt ggtacttatg gtgctatgcc
    16141 agaactcttc ttgaaactca atcagttgat tgcggataag gacctagaaa cagcgcgtga
    16201 attgcagtat gctatcaacg caatcattgg taaactcact tctgctcatg gaaatatgta
    16261 cggtgtcatc aaagaagtct tgaaaatcaa tgaaggcttg aatattggat ctgttcgttc
    16321 accattgaca ccagtgactg aagaagatcg tccagttgta gaagcggctg ctgccttgat
    16381 tcgtgaaacc aaggagcgct tcctctaatc taaaaggagg tatttatgac atattacgtt
    16441 gcaattgata tcggtggaac caacatcaag tatggtttgg ttgatcaaga ggggcaactt
    16501 cttgaatcgc atgaaatgcc aactgaggcg cataagggtg gacctcatat cttacaaaag
    16561 accaaagata tcgtagctag ttatttagaa aaaggcccag tagcaggtgt tgccatatct
    16621 tctgctggga tggtggatcc ggataagggt gagattttct atgctgggcc gcaaatccct
    16681 aactacgcag gcacccagtt caaaaaggaa atcgaagaaa gctttactat tccttgtgag
    16741 attgaaaatg atgtcaactg tgcaggtctt gctgaggcag tatctggttc aggcaaggga
    16801 gcaagtgtga cactttgctt gaccattgga accggtatcg gtggttgctt gattatggat
    16861 aggaaagtct tccatggttt tagcaattca gcctgtgaag tcgggtatat gcatatgcag
    16921 gatggagctt ttcaagactt ggcttctaca acagctttag tgaaatatgt agctgaagcc
    16981 catggagaag atgttgatca gtggaatggc cgtagaattt tcaaagaagc cactgaagga
    17041 aacaaaatct gcatggaagg tattgaccgt atggttgact atctaggaaa aggtctggca
    17101 aatatttgct acgttgccaa tccagaagtg gttattcttg gtggtggtat catggggcaa
    17161 gaggctatcc tcaaacctaa gatccgtaca gccttgaaag aggctttggt accaagttta
    17221 gcagaaaaaa cacgattaga atttgcccat caccaaaata cagcagggat gttgggtgca
    17281 tattatcatt ttaagacaaa acaatcctag tttggctcag ccaaactagg attttcttac
    17341 acgtttttgt ctacgatagc cgttgagttt tttattttcc cagtagctat taaagatttt
    17401 ttccttgctt tcgcgattga tttccaaaaa gtaggcataa atcaaatcga taaagaagag
    17461 cataggaagt tgagcggata ttcgttggat ataggagggt tggctgtggg tggctacaag
    17521 aacagtctct gtataggtct ggctatcttt attgggaaca cttgtaaaga gtacagtctt
    17581 tgcccccatc tccttagcat ctaatagact atctaaaata gaaggagttg agcctgaaag
    17641 tgagaagccc agtactagac aattttcatc catgatgctg gttgtccagg caaagccgtc
    17701 ttggtctgtc aaagcttcgc agaccacacc tagtcgcata aaacgtaatt tcatttcacg
    17761 ggcgacgagg ccagaactcc ctgttccaaa gaagtagata cgctcagcat cttcgattag
    17821 ctgggcaatt cgttctagtt ggatttcgtc aatcaagtct tgtgtttgtt ccctcatatt
    17881 gctataactt ctgaggactc gtttggtcag tggactgtgc ttggagactt ggttggcttg
    17941 attttctgcc tgatgttggt attggaaaat aaattctcgg tagccagtaa agccacactt
    18001 tttagcaaag cgggtcaaag cagcttgaga aatatgtaat ttttgggtga cttgttgaga
    18061 agataaatca tctgtaatcg tttcagcttg caaaaaatag cgagcgattt cttgttctag
    18121 gtctgtcatt tcttcaaaat gtgaatcaat gatagttgcg atatctggtt tgtccatagg
    18181 gaaagctcct ttacatgagt catactggaa gactagatca gagaatagtc acacttcatt
    18241 ataacacata atataaggat agataaataa aaacgcatct ctgttttaaa aacgaaaaaa
    18301 tcgaaaaagc ttctctcttt tccataattt tctactcaaa ttgtggtaca attaagagta
    18361 agattttaag ttagaaatga gactgatttg tatgagaaaa tttaacagcc attcgattcc
    18421 gattcggctt aatttattgt tttcaatcgt cattttactc tttatgacca ttattggtcg
    18481 tttgttgtat atgcaggttt tgaacaagga tttttacgaa aaaaagctag cttcagctag
    18541 tcagaccaag attacaagca gttcagcccg tggggaaatt tatgatgcta gtggaaaacc
    18601 tttggtagaa aatacgttaa agcaggttgt ttcctttacg cgtagcaata aaatgacggc
    18661 tacagactta aaagaaacag ctaaaaagtt actgacttat gtgagcatca gttctccaaa
    18721 tttgacagaa cgccagctgg cggattacta tttggctgat cctgaaatct ataaaaaaat
    18781 agtggaagct ctcccaagtg agaaacgctt ggattcagat ggcaatcgtc tatccgaatc
    18841 agaactgtat aacaatgcgg tcgatagtgt acaaacgagt caactaaact atacagagga
    18901 tgaaaagaaa gaaatctatc tttttagtca gttaaatgct gttggaaact ttgcgacagg
    18961 aaccattgcg acagatcctc taaatgattc tcaggtggct gttattgcct ctatttcaaa
    19021 ggagatgcct ggcattagta tttctacttc ttgggataga aaggttttgg aaacttccct
    19081 ttcttctata gttgggagtg tatccagtga aaaagctggt ctcccagcgg aagaagcaga
    19141 agcctatctt aaaaaaggct attctctaaa tggccgtgta ggaacctcct atttggaaaa
    19201 gcaatatgaa gagaccttac aaggaaaacg ctcggtaaaa gaaatccatc tggataaata
    19261 tggcaatatg gaaagcgtgg atacaattga ggaaggtagt aagggaaaca atatcaaact
    19321 gaccattgat ttggccttcc aagatagcgt ggatgcttta ctgaaaagtt atttcaattc
    19381 tgagctagaa aatggtggag ccaagtattc tgaaggtgtc tatgcagtcg cccttaaccc
    19441 aaaaacaggt gcggttttgt ctatgtcagg gattaaacat gacttgaaaa cgggagagtt
    19501 gacgcctgat tccttgggaa cggtaaccaa tgtctttgtt ccaggttcgg ttgtcaaggc
    19561 ggcgaccatc agctcaggtt gggaaaatgg agtcttgtca ggaaaccaga ccttgacaga
    19621 ccagtccatt gtcttccaag gttcagctcc catcaattct tggtatactc aggcttacgg
    19681 ttcattccct atcacagcgg tccaagctct ggagtattca tcaaatacct atatggtcca
    19741 aacagcctta ggtcttatgg ggcaaaccta tcaacccaat atgtttgtcg gcaccagcaa
    19801 tctagagtct gctatggaga aactgcgttc aacctttggc gaatatggct tgggtactgc
    19861 gacaggaatt gacctaccag atgaatctac tggatttgtt cccaaagagt atagctttgc
    19921 taattacatt actaatgcct ttgggcagtt tgataactat acgccgatgc agttggctca
    19981 gtatgtagca actattgcaa ataatggtgt tcgtgtggct cctcgtattg ttgaaggcat
    20041 ttatggtaat aatgataagg gaggactggg tgacttgatt cagcaactgc aaccgacaga
    20101 gatgaataag gtcaatatat ccgactccga tatgagcatc ttgcaccaag gtttttatca
    20161 ggttgcccat ggtactagtg gattgacaac tggacgtgcc ttttcaaatg gtgccttggt
    20221 atccattagc ggaaaaacag gtacagccga aagctatgtg gcagatggtc agcaagcaac
    20281 caataccaat gcggtggcct atgccccatc tgataatccc caaatcgctg tcgcagtggt
    20341 ctttcctcat aataccaatc taacaaatgg tgtaggacct tccattgcgc gtgacattat
    20401 caatctgtat caaaaatacc atccaatgaa ctagaaagga aattatgctt tatccaacac
    20461 ctattgccaa gttgattgac agttattcta agttaccagg tatcgggatt aagacggcta
    20521 cgcgtctggc cttttatacg attgggatgt ctgctgatga tgtcaatgaa tttgcaaaaa
    20581 atctcctttc tgctaagaga gaattgacat attgttctat ttgtggacgt ttgacagacg
    20641 acgatccttg ttctatctgt actgatccga ctcgtgacca gacaacaatt ttagttcttg
    20701 aggatagtag agatgtggca gccatggaaa atatccaaga ataccatgga ctctatcatg
    20761 tccttcatgg cctcatttct cctatgaatg gtatcagtcc ggacgatatc aatctcaaga
    20821 gccttatgac tcgtcttatg gatagtgagg tttcagaagt gattgtggcg actaatgcta
    20881 cagcggatgg tgaagcgact tccatgtatc tttcacgttt gctcaagccg gctggtatca
    20941 aggttacgcg tctagcacga ggtctcgctg tgggagcgga cattgagtat gcggacgaag
    21001 tgacactctt acgagccatt gaaaatcgga cagagttgta agtgtaggca aatttacgaa
    21061 ctccattcat ttataaaaaa tcaaagaggc tgaaaatcgt tcctatcggc ctctttttgt
    21121 atagtgtgat gagtaggctc aggttcaagt tttaaaaaac caagcaaata tgatatacta
    21181 aagagcgagt attctagtag aattaggaca aataatatga aacaaacgat tattctttta
    21241 tatggtggac ggagtgcgga acgcgaagtc tctgtccttt cagctgagag tgtcatgcgt
    21301 gcggtcgatt acgaccgttt cacagtcaag actttcttta tcagtcagtc aggtgacttt
    21361 atcaaaacac aggaatttag tcatgctccg gggcaagaag accgtctcat gaccaatgaa
    21421 accattgatt gggataagaa agttgcacca agtgctatct acgaagaagg tgcagtggtc
    21481 tttccagtcc ttcacgggcc aatgggagaa gatggctctg ttcaaggatt cttggaagtt
    21541 ttgaaaatgc cttacgttgg ttgcaacatt ttgtcatcaa gtcttgccat ggataaaatc
    21601 acgactaagc gtgttctgga atctgctggt attgcccaag ttccttatgt ggctatcgtt
    21661 gaaggcgatg atgtgactgc taaaatcgct gaagtggaag aaaaattggc ttatccagtc
    21721 ttcactaagc cgtcaaacat ggggtctagt gtcggtattt ctaagtctga aaaccaagaa
    21781 gaactccgtc aagccttaaa acttgccttc cgatatgaca gccgtgtctt ggttgagcaa
    21841 ggagtgaatg cccgtgaaat tgaggttggc ctcttgggta actacgatgt caagagcacg
    21901 ctacctggag aagttgtcaa ggacgttgcc ttttatgact acgatgccaa gtatattgat
    21961 aacaagatta ctatggatat tcctgccaaa atcagtgatg atgtggtggc tgtcatgcgt
    22021 caaaatgcag aaacagcctt ccgtgccatt ggtggccttg gtctatctcg ttgcgatttc
    22081 ttctatacag ataagggaga gatttttctc aacgagctca ataccatgcc aggttttacc
    22141 cagtggtcta tgtacccact actttgggac aatatgggga tcagctaccc agaactaatc
    22201 gaacgtttgg ttgaccttgc caaggaaagc tttgacaagc gcgaagcgca tttgatataa
    22261 aatgaaagag agggtagaag ccagaaccat cactgcacgg tgactagagt tctcggactt
    22321 cagccctttt taaaggagta gaaatgaaat taacaatcca tgaaattgcc caagttgttg
    22381 gagccaaaaa tgatatcagt atctttgagg acacccagtt agaaaaagct gagtttgata
    22441 gtcgtttgat tggaactgga gatttatttg tgccacttaa aggtgcgcgt gatggccatg
    22501 actttattga aacagccttt gaaaatggtg cagcagtaac cttgtctgag aaagaggtct
    22561 caaatcatcc ttacatttta gtagatgatg ttttgacagc ctttcaatcc ctagcatcct
    22621 actatcttga aaaaacgact gttgatgtct ttgctgttac aggttcaaat ggcaagacaa
    22681 cgactaagga tatgttggcg catttactgt caacaagata caagacctac aaaacacaag
    22741 gcaattacaa taatgagatt ggccttcctt acacagttct ccacatgcct gaaggaacag
    22801 aaaagttggt cttggagatg gggcaggacc acttgggaga tattcatctc ttgtctgaat
    22861 tggctcgtcc aaaaacagtc atcgtgacct tggttggaga agctcatttg gcctttttca
    22921 aagatcgttc tgagattgct aaaggaaaaa tgcaaattgc agacggaatg gcttcaggtt
    22981 ccttgttttt agcgccggct gaccctatcg tagaggacta tttgccaact gataaaaagg
    23041 tggttcgttt tggtcaagga gcagagctgg aaattactga cttggttgag cgcaaagata
    23101 gtctgacctt caaggccaat ttcttagagc aagtccttga tttgccagta actggcaagt
    23161 acaatgcgac aaatgctatg attgcatcct atgttgcctt gcaagaagga gtttcagagg
    23221 agcaaattcg tttggccttc caacatcttg aattgacgcg taaccgtacc gagtggaaga
    23281 aagcagtcaa tggagcagat atcctgtcag atgtttacaa tgccaatcca actgctatga
    23341 aactgatttt agagactttc tctgccattc cagccaatga aggtggcaag aaaattgcag
    23401 ttttggcgga tatgaaggag cttggtgacc agtctgttca acttcataat cagatgattt
    23461 tgagcctctc cccagatgtg cttgatatcg ttattttcta tggagaagat attgctcagt
    23521 tggctcaatt ggccagtcaa atgtttccaa tcggccacgt ttactacttc aagaaaacag
    23581 aagaccagga tcaatttgaa gacctagtca agcaggttaa ggaaagcctt ggagcctatg
    23641 accaaatctt gctaaaaggt tctaactcta tgaatctagc caaattggta gaaagtttag
    23701 aaaatgaagc caagtgattt tgttaagtat ctgcaaagaa tgattgccct tacagatact
    23761 ggcttaacct ttacaaaaga tccttttgac cgtgagcgct acgaggactt gcgaagtctg
    23821 ttatctgaaa tgttgaatca gggatcagac ctggatgtag aagaagtagc agaagtcttg
    23881 aaaccaactt cagcttatgc gactccctta atggacgtcc gtgcttggat tgttgaggat
    23941 gagaagattt gtctggttag gggacaagga gaggatagtt gggctttgcc aggtggcttt
    24001 ggtgaagtcg gctattctcc aactgaaaat attctcaagg aaattgaaga agaaaccggt
    24061 tttaaagcca aagttgaaag actgctagct gtttttgata ccaatcgttt ccaactacag
    24121 agcaaacaat atgcaaagtt tgtttttgaa tgtaagcttc tcgatggaca attccaagaa
    24181 aatcaagaaa tcgctgacct tcaatttttt gcaattgacc aactgccgaa tttatctgaa
    24241 aaacgcatta ccaaggagca aatcgagatt ctttggcagg tttatcaagg tcagagggag
    24301 caatatcttg actaagaaga tgattatcgt atttctaaat ccatttttga caactagcat
    24361 ggtataataa tatgcaggaa aattttgaat tatgaggaag actagatgaa tttatgggat
    24421 attttcttta cgactcaggc aaccgagccg cccaaatttg accttttttg gtatgttagc
    24481 ctatttacgc tcttagcctt aaccttttat acagcccatc gctatcgtga aaagaaggtt
    24541 taccaacgat ttttccaaat cttgcagact gttcagttaa tccttcttta tggttggtac
    24601 tgggttaatc atatgccact gtcagaaagc ctaccctttt accattgccg tatggctatg
    24661 tttgtggtac tcttgcttcc tggtcagtcc aaatataaac aatactttgc attattggga
    24721 acatttggga cattagcagc ctttgtttat ccagtgccag atgcttaccc ttttccacat
    24781 gtcaccattc tatcctttat ctttggtcat ttagcactct tggggaactc tctagtttat
    24841 ctattgagac agtataatgc gcgattgctg gatgtgaagg gaatttttct catgaccttt
    24901 gccctaaatg ccttgatttt tgtggtcaat ttggtgacag gtggcgatta cggatttttg
    24961 acaaaaccgc cattggttgg ggatcacggt ctagtagcta attatttact tgtttcaatt
    25021 gtgctggtag ctactatcag tttgactaag aaaatcttag aattcttttt agctcaagaa
    25081 gcagaaaaaa tgattgcaaa ggaagcttaa cacagagctt tcttttttgc tcttagagag
    25141 tttttacaag cagcttataa aataagaatt tctgaataga caaactcaaa aaatggctgg
    25201 gaaatttagg aaaaaagcaa gcacgattaa attttttgtg ttataatatt ttgtgaatag
    25261 ctatgcctat gtttagctat ggaataatac gaagtgcgaa acttggaaga tagagaggaa
    25321 gcgatgtaat ggctagagaa ggctttttta caggtctaga tattggaaca agctctgtca
    25381 aggtgcttgt ggccgagcag agaaatggtg aattaaatgt aattggcgtg agtaatgcca
    25441 aaagtaaagg tgtaaaggat ggaattattg ttgatattga tgcagcagca actgctatca
    25501 agtcagccat ttcccaagcg gaagaaaagg caggcatttc gattaaatca gtgaatgtcg
    25561 gcttgcctgg taatcttttg caggtagaac caactcaggg gatgattcca gtaacatctg
    25621 atactaagga aattacggat caagatgttg aaaatgttgt caaatcagct ttgacaaaga
    25681 gtatgacacc tgaccgtgaa gtcattacct ttattcctga agaatttatt gtggatggtt
    25741 tccaagggat tcgtgaccca cgtggtatga tgggggttcg ccttgaaatg cgtggtttgc
    25801 tttatacagg acctcgtact atcttgcaca atttgcgtaa gacggttgag cgtgcaggtg
    25861 ttcaggttga aaatgttatc atttcaccac tagcaatggt tcagtctgtt ttgaacgaag
    25921 gggaacgtga atttggtgct acagtgattg atatgggggc aggtcaaacg actgtcgcta
    25981 caatccgtaa tcaagaactc cagttcacac atattctcca agaaggtgga gattatgtaa
    26041 ctaaagatat ctccaaggtt ttgaaaacct ctcgcaaatt agcggaaggc ttgaaactga
    26101 attacgggga agcctatccg cctcttgcaa gcaaagaaac cttccaagta gaggttattg
    26161 gagaagtgga agcagtcgaa gtgacggaag cctacttgtc agaaattatt tctgcacgaa
    26221 tcaagcacat ccttgaacaa atcaagcaag aattagatag aaggcgtcta ttggacctcc
    26281 ctggtggtat tgtcttaatc ggtgggaatg ccattttacc aggtatggtt gagcttgctc
    26341 aggaagtctt tggcgtccgt gtcaagcttt atgttccaaa tcaagttggt atccgtaatc
    26401 cagcctttgc gcatgtgatt agtttatcag aatttgcggg tcaattaaca gaagttaatc
    26461 ttttggctca gggagcgata aaaggtgaga atgacttaag tcatcagcca attagttttg
    26521 gtgggatgct gcaaaaaaca gctcagtttg tacaatcaac gcctgttcaa ccagcacctg
    26581 ctccagaagt agagccggtg gcgcctacag aaccaatggc ggatttccaa caagcttcac
    26641 aaaataaacc gaaattagca gatcgtttcc gtggcttgat cggaagcatg tttgacgaat
    26701 aaagaggaaa aataaattat gacattttca tttgatacag ctgctgctca aggggcagtg
    26761 attaaagtaa ttggtgtcgg tggaggtggt ggcaatgcca tcaaccgtat ggtcgacgaa
    26821 ggtgttacag gcgtagaatt tatcgcagca aacacagatg tacaagcatt gagtagtaca
    26881 aaagctgaga ctgttattca gttgggacct aaattgactc gtggtttggg tgcaggaggt
    26941 caacctgagg ttggtcgtaa agccgctgaa gaaagcgaag aaacactgac ggaagctatt
    27001 agtggtgccg atatggtctt catcactgct ggtatgggag gaggctctgg aactggagct
    27061 gctcctgtta ttgctcgtat cgccaaagat ttaggtgcgc ttacagttgg tgttgtaaca
    27121 cgtccctttg gttttgaagg aagtaagcgt ggacaatttg ctgtagaagg aatcaatcaa
    27181 cttcgtgagc atgtagacac tctattgatt atctcaaaca acaatttgct tgaaattgtt
    27241 gataagaaaa caccgctttt ggaggctctt agcgaagcgg ataacgttct tcgtcaaggt
    27301 gttcaaggga ttaccgattt gattaccaat ccaggattga ttaaccttga ctttgccgat
    27361 gtgaaaacgg taatggcaaa caaagggaat gctcttatgg gtattggtat cggtagtgga
    27421 gaagaacgtg tggtagaagc ggcacgtaag gcaatctatt caccacttct tgaaacaact
    27481 attgacggtg ctgaggatgt tatcgtcaac gttactggtg gtcttgactt aaccttgatt
    27541 gaggcagaag aggcttcaca aattgtgaac caggcagcag gtcaaggagt gaacatctgg
    27601 ctcggtactt caattgatga aagtatgcgt gatgaaattc gtgtaacagt tgttgcaacg
    27661 ggtgttcgtc aagaccgcgt agaaaaggtt gtggctccac aagctagatc tgctactaac
    27721 taccgtgaga cagtgaaacc agctcattca catggctttg atcgtcattt tgatatggca
    27781 gaaacagttg aattgccaaa acaaaatcca cgtcgtttgg aaccaactca ggcatctgct
    27841 tttggtgatt gggatcttcg ccgtgaatcg attgttcgta caacagattc agtcgtttct
    27901 ccagtcgagc gctttgaagc cccaatttca caagatgaag atgaattgga tacacctcca
    27961 tttttcaaaa atcgttaagt aaatgaatgt aaaagaaaat acagaacttg tttttcgaga
    28021 agttgcagag gctagtctga gtgctcatcg agagagtggt tcggtctctg tcattgcagt
    28081 taccaagtat gtagatgtac cgacagcgga agccttgctt ccgctaggtg tccatcatat
    28141 cggtgaaaat cgtgtagata agtttctgga aaaatatgaa gctttaaaag atcgagatgt
    28201 gacttggcat ttgattggta ccttgcaaag acgtaaggtg aaagatgtca ttcaatacgt
    28261 tgattatttc catgcattgg actcagtaaa gctagcaggg gaaattcaaa aaagaagtga
    28321 ccgagtcatc aagtgtttcc ttcaagtaaa tatttctaaa gaagaaagca aacacggttt
    28381 ttcgagagag gaactgctgg aaatcttgcc agagttagcc agactagata agattgaata
    28441 tgttggttta atgacgatgg caccttttga ggctagcagt gagcagttga aagagatttt
    28501 caaggcggcc caagatttac aaagagaaat tcaagagaaa caaattccaa atatgcctat
    28561 gaccgagtta agtatgggaa tgagtcgtga ttataaagaa gcgattcaat tcggttccac
    28621 ttttgttcgt ataggtacat cattttttaa gtaggagaga accatgtctt taaaagatag
    28681 attcgataga tttatagatt attttacgga ggatgaggat tcaagtctcc cttatgaaaa
    28741 aagagatgag cctgtgttta cttcagtaaa ttcttcacag gaaccggctc tcccaatgaa
    28801 tcaaccttca cagtcggctg ggacaaaaga gaacaatatc accagacttc atgcaagaca
    28861 acaggaattg gcaaatcaga gtcagcgtgc aacggataag gtcattatag atgttcgtta
    28921 tcctagaaaa tatgaggatg caacagaaat tgttgattta ttggcaggaa acgaaagtat
    28981 cttgattgat tttcagtata tgacagaggt gcaggctcgt cgttgtttgg actatttgga
    29041 tggagcttgt catgttttag ctggaaattt gaaaaaggta gcttctacca tgtatttgtt
    29101 gacaccagtg aacgttattg taaatgttga agatatccgt ttaccagatg aagatcaaca
    29161 gggtgagttc ggttttgata tgaagcgaaa tagagtacga taatgatttt tttaattcgt
    29221 atgatttata atgcagtgga tatttactcc ctaattttgg tagccttcgc tgtcatgtct
    29281 tggtttccag gtgcctacga atccagttta ggtcgttgga ttgtagcgtt ggtaaaacca
    29341 gtgcttgctc ccttgcaacg cctgccttta cagatagcgg gtcttgattt atctgtttgg
    29401 gtcgcgattg ttttggttcg atttttagga gaaaacctag tgcgttttct ggcgatgata
    29461 ggatgaataa agggatttat cagcatttct ccatagaaga tcgtccattt cttgacaagg
    29521 gaatggaatg gataaagaag gtagaagata gctatgctcc ttttttaact ccttttatca
    29581 atcctcatca ggagaagcta ttaaagattt tggccaaaac ctatggtctt gcttgtagca
    29641 gtagtgggga attcgtctcg agtgagtatg ttcgagtttt attataccca gattatttcc
    29701 aaccagagtt ttcagatttt gaaatatctc tccaggaaat tgtgtattcc aataaatttg
    29761 aacatttaac gcatgctaag attttaggga cagtcatcaa tcaattaggg attgaacgga
    29821 aactttttgg agatatccta gtagatgaag aacgggcgca gattatgatt aatcagcagt
    29881 ttcttcttct ctttcaagat ggactaaaga aaattggtcg tatacctgtt tcgctggagg
    29941 aacgtccttt caccgagaaa atagataagc tagaacagta tcgagaactg gatttatctg
    30001 tgtctagttt tcgattagat gttcttttat caaatgtttt gaaactatct aggaatcaag
    30061 caaaccagtt gattgaaaag aaacttgtcc aagtaaatta tcatgtggta gacaaatcag
    30121 attacactgt tcaagttgga gacttgatta gtgtgagaaa atttggtcgc ttgagattac
    30181 ttcaagataa gggacaaacg aaaaaagaga agaaaaaaat aaccgtccag ttattattaa
    30241 gtaagtgagg aatagaatgc caattacatc attagaaata aaggacaaga cttttggaac
    30301 tcgattcaga ggttttgatc cagaagaagt cgatgaattt ttagatattg tggttcgtga
    30361 ttacgaagat cttgtgcgtg cgaatcatga taaaaatttg cgtattaaga gtttagaaga
    30421 gcgtttgtct tactttgatg aaataaaaga ttcattgagc cagtctgtat tgattgctca
    30481 ggatacagct gagagagtga aacaggcggc gcatgaacgt tcaaacaata tcattcatca
    30541 agcagagcaa gatgcgcaac gcttgttgga agaagctaaa tataaggcaa acgagattct
    30601 tcgtcaagca actgataatg ctaagaaagt cgctgttgaa acagaagaat tgaagaacaa
    30661 gagccgtgtc ttccaccaac gtctcaaatc tacaattgag agtcagttgg ctattgttga
    30721 atcttcagat tgggaagata ttctccgtcc aacagctact tatcttcaaa ccagtgatga
    30781 agcctttaaa gaagtggtta gcgaagtact tggagaaccg attccagctc caattgaaga
    30841 agaaccaatt gatatgacac gtcagttctc tcaagcagaa atggaagaat tacaagctcg
    30901 tattgaggta gccgataaag aattgtctga atttgaagct cagattaaac aggaagtgga
    30961 aactccaact cctgtagtga gtcctcaagt tgaagaagag cctctgctca tccagttggc
    31021 ccaatgtatg aagaaccaga agtagctcca gtgcatccga caggtccaac accagctaca
    31081 gaaactgttg attcagcacc aggatttgaa gcaccgcaag aatccgttac aattttataa
    31141 gaaacgatgt gagaacaata tcttatcctt atatttccag cgagcaggag atggtgtgag
    31201 tcctgcattc cctaacgata agattatcct ctcaaaaact caagtctgaa tctagtaaga
    31261 tttgacgttt cccacgttac gggataagag ggagaaagac taaatctttt tccgaataaa
    31321 ggtggtacca cgattttcgt cctttttgga agtcgtggtt tttaatttgt tattatttat
    31381 aaaggagata ccatgaaact aaaagatacc cttaatcttg ggaaaacagc tttcccaatg
    31441 cgtgcaggcc ttccgaccaa agagccagtt tggcaaaagg aatgggaaga tgcaaaactt
    31501 taccaacgtc gtcaagaact aaatgaaggg aaaccgcatt ttgtcttgca tgatggccct
    31561 ccatacgcaa acggaaatat ccacgttgga catgccatga atcatatctc aaaagatatc
    31621 atcattcgtt ctaagtctat gtcaggtttt aacgcacctt atatcccagg ttgggatact
    31681 catggtctgc caatcgagca agttttggca aagcaaggtg tgaagcgcaa agaaatggac
    31741 ttggttgagt acttgaaact ctgtcgtgag tacgctcttt ctcaagttta taaacaacgt
    31801 gatgatttca aacgcttggg gatgtcaggt gactgggaaa atttatatgt gactttaact
    31861 ccagactatg aagcagccca aatccgtgtc tttggtgaga tggctaataa gggttatatc
    31921 taccgtggtg ctaagccagt ttactggtca tggtcatctg agtcagccct tgccgaagcg
    31981 gaaattgaat accatgattt ggtttcgact tcactttatt atgccaataa ggtaaaagat
    32041 ggcaaaggta tcctagatac agatacttat atcgtagtct ggacaacaac tccatttacc
    32101 atcacagctt ctcgtggttt gactgttggt gcagatattg attacgtttt ggttcaacct
    32161 gctggtgaaa ctcgtaagtt tgtcgttgct gcagaattgt tgactagctt gtctgagaaa
    32221 tttggctggg ctgatgttca agtgttggca acttaccgtg gtcaagaact caaccacatc
    32281 gtaacagaac acccatggga tacagctgta gatgagttgg taatccttgg tgatcacgtt
    32341 accactgact ctggtacagg tatcgtccat acagcccctg gttttggtga ggacgactac
    32401 aatgttggta ttgctaatgg tcttgaagtc gcagtgactg ttgacgaacg cggtatcatg
    32461 atggcgaatg ctggtcctga gtttgaaggt cagttctacg acaaggttgt accaactgtt
    32521 attgaaaaac ttggtaacct ccttctagcc caagaagaaa tctctcactc atacccattt
    32581 gactggcgta ctaagaaacc aatcatctgg cgtgcagttc cacaatggtt tgcctcagtt
    32641 tcaaaattcc gccaagaaat cttggatgct attgataaag ttaaattcca tacagaatgg
    32701 ggtaaagtac gtctatataa tatgattcgt gaccgtggtg actgggttat ctctcgtcaa
    32761 cgtgcttggg gtgttccact tccaatcttc tatgcagaag acggtacagc tatcatgaca
    32821 gctgagacta ttgaacatgt ggcgcaactc tttgaagtac atggttcaag catttggtgg
    32881 gaacgtgatg ccaaggacct cttgccagaa ggatttacac atccaggttc tccaaatggg
    32941 gagttcaaga aagaaacaga tattatggat gtatggtttg actcaggttc atcatggaat
    33001 ggtgttttgg taaatcgtcc aaatctgact tatccagctg acctttacct tgaaggttct
    33061 gaccaatacc gtggttggtt caactcatca ctcattacat ctgttgccaa ccatggcgta
    33121 gcaccttaca aacaaatctt gtcacaaggt tttacccttg atggtaaagg tgagaagatg
    33181 tctaaatcac ttggcaatac cattgctcca agtgatgttg aaaaacaatt tggtgcagaa
    33241 atcttgcgtc tctgggtaac aagtgttgac tcaagcaatg acgtgcgtat ctctatggat
    33301 attttgagcc aagtttctga aacttaccgt aagattcgta acactcttcg tttcttgatt
    33361 gccaatacat ctgactttaa cccagctcaa gatgtagtcg cttacgatga gcttcgttca
    33421 gttgataagt acatgacgat tcgctttaac cagcttgtca agaccattcg tgatgcctat
    33481 gcagactttg aattcttgac gatctacaag gccttggtga actttatcaa cgttgacttg
    33541 tcagccttct accttgattt tgccaaagat gttgtttaca ttgaaggtgc caaatcactg
    33601 gaacgccgtc aaatgcagac tgtcttctat gacattcttg tcaaaatcac caaactcttg
    33661 acaccaatcc ttcctcacac tgcggaagaa atttggtcat atcttgagtt tgaaacagaa
    33721 gacttcgtcc aattgtcaga attaccagag gctcaaactt ttgctaatca agaagaaatc
    33781 ttggatacat gggcagcctt catggacttc cgtggacaag ctcaaaaagc cttggaagaa
    33841 gctcgtaatg caaaagtaat cggtaaatca cttgaagcac acttgacagt ttatccaaac
    33901 gaagttgtga aaactctact cgaagcagta aacagcaatg tggctcaact tttgatcgtg
    33961 tcagacttga ccatcgcaga aggaccagct ccagaagctg cccttagctt cgaagatgta
    34021 gccttcacag ttgaacgcgc tgcaggtgaa gtatgtgacc gttgccgtcg tattgaccca
    34081 acaacagcag aacgtagcta ccaggcagtt atctgtgacc actgtgcaag tatcgtagaa
    34141 gaaaactttg cagatgcagt cgcagaagga tttgaagaga aataagattg aaaagtctag
    34201 gcaaaattca atttgagaag aaaagacaac taattttata gtctattaaa cgcattgtat
    34261 cacgtttttg aacacctgat atgatgcgtt ttttattttt aaaatttgca aggtgtgact
    34321 ttttatactc atactcaatg aaaatcaaaa agcaaactag gaagctagcc gcaggctgct
    34381 caaaacactg ttttgaggtt gtagataaga ctgacgaagt cagtaacata tatatggtaa
    34441 ggcgacgctg acgtggtttg aagagatttt cgaagagtat caacaagaat caaagagaaa
    34501 cttagcaagc taacagtagt aagataaaat aggaatttga tattagggat aagattggta
    34561 aatagtgtaa tatttttaca acaataaatt tatatagtta tttctggttt ctgaaaagta
    34621 ttatattata ttttatttca tattatacaa atttttattt tataatatca gaaaatactt
    34681 tttttaaaaa gcaaatatga tataatttta tttgaaaaaa aataaaaaag gagattttat
    34741 tatgaaatca agtggtatcg tcggtcttgt gggaggaatt ttacttctta tttgtccttt
    34801 tgtcttgttg ggaacagcgg taaatacagc tgctacaact cttaatggag gagctactgc
    34861 aggggctttt tcaggtgttg ccttactctt gaataccttg aagattgcaa atcttgttct
    34921 tggtatcatt gctattgttt actataaagg agataagcat gtaggtgcag ctccgtctgt
    34981 actaatgatt gtttctggtg aagttagtct cattccattc ttaggatggg ttggggggat
    35041 tcttgctatt atcggaggat ctctattcct tgcaacattg aagaaattca aatcagaaga
    35101 ataaaaggta ttttagcatg aaaagaacaa aaaagtttat cggtatagga gtagctctat
    35161 tatctctttc tcttctagtt gcatgtggaa cataaagttc aaagaatact tcaacaagta
    35221 atgatgagaa gacagtagca acatccaata gttcaaaaga aacaatcact ttcgatacac
    35281 cggttgtaac agacgatgcg attgaatcaa tacgcactta tgcagattat atagatcttt
    35341 ataaaaatat ttttgatgat tattttacta aagctgagga aggtttcaaa ggcacagcta
    35401 tggaaaataa tgactcgttt actaaactaa aagagtcaac tcaaaaatta ttcgatgcgc
    35461 agaaaaaaag gttaaataaa tgaagataga atagaaacaa ccaaaaacaa tgtgattgcc
    35521 aaacattgtc aaacagtcct ttcctttttg gttttgacta gcttttttgt gaaaaattgt
    35581 gtaaaataga atagataaac gaggggaaac ctcggaaaat ttaaaggaga atccatctaa
    35641 tggtaaaatt ggtttttgct cgccacggtg agtctgaatg gaacaaagct aaccttttca
    35701 ctggttgggc tgatgttgat ttgtctgaaa aaggtacaca acaagcgatt gacgctggta
    35761 aattgatcaa agaagctggt atcgaatttg accaagctta cacttcagta ttgaaacgtg
    35821 ctatcaaaac aactaacttg gctctcgaag cttctgacca attgtgggtt ccagttgaaa
    35881 aatcatggcg cttgaacgaa cgtcactacg gtggtttgac tggtaaaaac aaagctgaag
    35941 ctgctgaaca atttggtgat gagcaagttc acatctggcg tcgttcatac gatgtattgc
    36001 ctccaaacat ggaccgtgat gatgagcact cagctcacac agaccgtcgt tacgcttcac
    36061 ttgacgactc agttatccca gatgctgaaa acttgaaagt gactttggaa cgtgctcttc
    36121 cattctggga agataaaatc gctccagctc ttaaagatgg taaaaacgta ttcgtaggag
    36181 ctcacggtaa ctcaatccgt gcccttgtaa aacacatcaa aggtttgtca gatgacgaga
    36241 tcatggacgt ggaaatccct aacttcccac cattggtatt cgaattcgac gaaaaattga
    36301 acgtcgtttc tgaatactac cttggaaaat aaaaaattgt aagtctagaa ttgatttcta
    36361 ggctttttat gttagtatgg aagtatgata aggaataaaa aacaagatta tgtactggcc
    36421 tacaagcaac cagcttcaac cacttacatg ggttgggagg aagaagcttt accgataggc
    36481 aatggttctt taggagcaaa agtatttggc cttatagggg ctgaacggat tcaatttaat
    36541 gaaaaaagtc tctggtctgg aggtccactt cctgatagtt cagattatca gggtggaaat
    36601 cttcaggatc agtatgtttt tttagctgag attcggcagg ctttggagaa gagagattac
    36661 aatctggcta aggaactggc tgagcagcac ctaattgggc caaaaacgag tcaatatggg
    36721 acctatctgt cttttgggga tattcacatt gagttcagcc agcaaggtac gactttgtct
    36781 caggtgacgg actatcagag acagttgaat attagtaagg cacttgcgac gacttcttat
    36841 gtctataagg gaacgcgatt tgaacgtgaa gcttttgcga gttttccaga tgatctcttg
    36901 gttcaatgtt ttactaagga agggttggaa actctagatt ttactataga actatccttg
    36961 acctgtgatt tggcttctaa tggaaagtat gagcaggaaa aatctgatta caaggagtgt
    37021 aagttggata ttactgattc tcatatcttg atgaagggaa gagttaagga taatgatctg
    37081 cggtttgcta gttatctagc ttgggaaacg gatggagata ttagagtttg gtcagatagg
    37141 gttcagatat caggagccag ttatgccaat ctcttcttgg ccgctaagac ggattttgcc
    37201 caaaatcctg ctagcaatta tcgcaagaaa ctagatttag agcaacaggt gatagacttg
    37261 gtggacacag ctaaagaaaa gggctatacc caattgaaat caaggcatat cgaggactac
    37321 caagccttat tccagcgtgt tcaattggat ttggaagctg atgttgacgc atccactaca
    37381 gatgatttgt taaaaaatta taagccacaa gaagggcagg ctttggagga gctgttcttc
    37441 cagtatggac ggtatttatt gattagttcg tccagatact gcccagatgc tctaccagct
    37501 aacctacagg gagtctggaa tgcggtcgac aatcctcctt ggaattcgga ctatcactta
    37561 aatgtcaatc tgcagatgaa ttattggcca gcctatgtta ccaatcttct agagacggtc
    37621 tttccagtca tcaattatgt agatgatttg cgtgtctatg gtcgtctagc ggctgtaaag
    37681 tatgcaggaa tcgtctctca gaaaggtgag gagaatggtt ggttggttca tactcaagcg
    37741 actccctttg gttggacggc acctggttgg gattactatt ggggttggtc accagctgcc
    37801 aacgcgtgga tgatgcaaac cgtttatgaa gcctattcat tttataggga ccaagactat
    37861 ctcagggaga aaatttatcc catgttgagg gaaacggttc gtttttggaa tgccttttta
    37921 cataaggatc agcaggcgca gcgttgggtg tcttctccgt cttattcccc agaacatggg
    37981 ccgatttcga ttggcaatac ctatgaccaa tctctgattt ggcagttatt tcatgatttt
    38041 attcaggctg ctcaggaatt gggactggat gaggacttgt tgactgaggt taaggagaag
    38101 tctgatttac taaatccttt gcaaatcact caatctggtc gaatcaggga gtggtatgag
    38161 gaggaagagc agtattttca aaatgagaaa gtggaggccc agcatcggca cgcttcccat
    38221 ctagtgggac tctatcctgg caatctcttt agctacaagg gacaagagta tattgaagcg
    38281 gcgcgtgcta gcctcaatga tcgtggagat ggcggcacag gctggtccaa ggctaataag
    38341 atcaatctct gggcgcgttt gggagatggt aatcgagccc ataaattatt ggcagagcag
    38401 ttaaagacat ccaccttgcc aaatctttgg tgtagccatc ctccttttca gatagatggt
    38461 aattttggtg ctactagtgg catggcagaa atgttactcc agtctcatgc agcttatctg
    38521 gtacctctag ctgccttacc tgatgcttgg tcaacaggtt ctgtttcagg cttaatggca
    38581 cgtggacatt ttgaagtgag catgagctgg gaagataaaa aactcttaca gttgaccatt
    38641 ttatcaagga gtggaggaga tttgcgagtt tcttatccag atattgagaa gagtgtgatt
    38701 aaaatgaatc aagaaaaaat aaaagcgaaa tgcatgggga aagattgtat ttcggtggca
    38761 acagcagaag gtgatcttgt tcaattttat ttttaagaag atgttataag gcagtaattt
    38821 gaaactgcct tttaataagg atttaagaat gtaagcagtt tccaactagt tgaaaaagcg
    38881 ttataatgat aataggaagt aatactcaat gaaaatcaaa gagcacaaac taggaagcta
    38941 gccgcaggtt gctcaaaaca gtgttttgag gttgcagatg gaagctgacg tggtttgaag
    39001 agagattttc gaggagtata atttgtttga tagagggtgg gtctgatggc ttatattgag
    39061 atgaaacact gttacaagcg ttatcaggtt ggggacacgg agattgtggc caatcgtgat
    39121 gtgaattttg agattgaaaa gggggagctg gttattatcc ttggtgcttc aggtgcaggt
    39181 aagtcaacag ttcttaacct tcttggggga atggatacca atgatgaagg ggaaatctgg
    39241 attgatggtg ttaatattgc ggattatagt tcccaccagc gcaccaatta ccgtagaaat
    39301 gatgtggggt ttgtttttca gttttataat ctagtttcta atctgacagc taaggaaaat
    39361 gtggaattgg cttctgaaat tgtgacagat gccttgaatc ctgatcaggt cttgacagat
    39421 gtaggtctgg ctcatcgtct caataacttt ccagcccagc tttctggagg ggagcaacag
    39481 cgagtctcca ttgcacgcgc ggtagccaaa aatcctaaaa ttcttctttg tgatgaacct
    39541 actggagcct tggattatca gacgggcaag caggttttga aaattctcca agatatgtct
    39601 cgtcaaaagg gagcgacggt gatcatcgtg actcataatg gagctttggc gcccattgct
    39661 gatcgcgtga ttcatatgca cgatgccagt gtcaaggatg tggtgctcaa ccagcatcct
    39721 caggatattg acagtttgga gtactagtat gatcaagcga aaaacttatt ggaaggactt
    39781 aattcagtcc ttcacaggct ccaaggggcg ttttttatcc atcttgatcc tgatgatgtt
    39841 gggatctcta gccttagtag gcctcaaagt aaccagtccc aacatggagg cgacagctaa
    39901 tgcttattta acaactgctc aaaccttgga tttggcagtc atgtctaact atggcttgga
    39961 tcaagcagac caagaagaac taaaacagac ggagggcgca gaggtcgagt ttggctattt
    40021 gacagatgtg actatggata atgggcagga tgccattcgg ctgtactcca aaccagagcg
    40081 aatttcaacc tttcagctaa gaaagggacg acttcctcag tcagacaagg aaatcgcttt
    40141 ggccactcat ttgcaaggcc aatacagcgt gggacaggag attagtttta aagaaaaaga
    40201 agagggtcat tcctctttaa aagaccatac ttataccatt actggttttg tggattcggc
    40261 tgaaatcctc tcccagcgag atatgggcta cgcaggaagt ggaagtggga ctctgacagc
    40321 ctatggggtg attttaccta gtcagtttga tcagaaagtc tacaatatag ctcgtttgaa
    40381 atatcaagat ttagcaggtt taaatgcctt ttcatcagct tatgaagaaa aatccaagca
    40441 acatcaggaa gagcttgaac aaattttatc agataatggc aaggtacgtc tgcaactttt
    40501 gaaaaaagaa ggacaagagt ctctagacaa ggggcaagag acccttgaca aggctcagac
    40561 taatttgcag gaaggcaagc gtcgtttagc agctgctcaa gctcgtatac aggctcaaga
    40621 aagtcaacta gccttgtttc ctcaagttca gagagagcag gctagtgctc aacttaccca
    40681 agccaagcag gaattgggca aggaagaggc caaactaaag caagctgaac aaaatctagc
    40741 ccaagaaaat gaaaaattag aaaaacatca gcaagtcttg gacgatttgg cggagccaag
    40801 gtatcaggtc tataatcgtc agaccatgcc aggtggtcag ggctatctta tgtatagcaa
    40861 tgcttcatcc agtattcgag cagtgggcaa tatctttcct gtggtacttt atgccgtagc
    40921 agccatggtg acctttacga ccatgactcg ctttgtagac gaagagcgaa ctcatgcagg
    40981 gatttttaag gccttgggtt atcgtagtaa ggatattatc gccaagtttc tcctttatgg
    41041 actagtagct gggactgtcg gaacggctct aggtagtata cttggtcatt atttgctagc
    41101 cagtgtaatt tcaagtgtca ttacaaaagg catggtggtg ggagaaactc agattcagtt
    41161 ctattggacc tatagcttac tagctcttgt cttgagcttg ttggcgagtg tgttaccagc
    41221 ctatctggtg gcttggaggg aacttcatga cgaagcagcc cagcttctac ttcctaaacc
    41281 tcctgtcaaa ggagctaaaa tcttattgga gcgtatcggt tttatctggc gtcgtctcag
    41341 ttttactcat aaggtaacag cccgcaacat ctttcgttat aagcagagaa tgttgatgac
    41401 aatctttggt gtggcaggtt ctgtagctct gctctttgca ggtttgggaa tccaatcttc
    41461 tgtagcagga gttccgtcta aacagtttca acaaatccaa cagtatcaga tgcttgtctc
    41521 tgaaaatcct agtgcgacca atcaggacaa ggtagagcta gcagaagtgt tgaaagggca
    41581 ggagatacta gcctaccaga aaatctattc taaaacgcta gacaaggatt tcaaaggcaa
    41641 ggctggtctt caaaacatta ctcttatgat gatagagaag gaagatttga ctccctttat
    41701 ccatcttcaa catcatcagc aggagctgac attaaaagat ggcatcgtta ttacagctaa
    41761 actcgcccag ctggcaggtg tcaaggttgg gcagacttta gaaattgaag gtaaggaact
    41821 aaaggtcgct gctattactg agaactacgt tggtcacttt atttatatga gtcaggctag
    41881 ctatgagcaa ctttacggac agctacccca agccaacact tatctggtct cattaaggga
    41941 taccagtgca actagtatcg aaagtcaggc gggcttgctt atgaatcaat ctgcggtgtc
    42001 cagcgttgtc caaaatgctt cagccattcg actcttcgac tctatcgcta gctcacttaa
    42061 tcagaccatg accatcttgg tcatcgtatc tgttctatta gctattgtca tcctttacaa
    42121 tctgaccaat atcaacgtag ctgagagaat ccgtgaactc tccactatca aggttctcgg
    42181 ttttcataat aatgaagtca cactctacat ttaccgtgag acgattgtgc tgtcccttgt
    42241 gggaatcgta cttggtctga tagctggttt ctatttacac caatttttga ttcaaatgat
    42301 ttcgcctgcg actattctct tttatccgca ggtaggctgg gaagtctatg taatcccagt
    42361 ggcagcagta agcatcattt tgaccttgct tggtttcttc gtcaattatt atctgagaaa
    42421 ggttgatatg ttagaagctc tgaaatctgt agagtaaggt agttattttt agctgattga
    42481 acttctattt actaatattc aaaaatcctc cgtttcaaag agcagggaac tctttgtgac
    42541 agaggatttt ttctataggg ctttagcagc tgcaattgcg gcttcgaagt ttggctcaga
    42601 attgatatta tccacgtatt caacgtagcg aatcgtattg tcagtatcga ggacaaagac
    42661 tgcgcgtgct aataggtgcc attcgttgat taagagggca taatcgtgcc cgaaagaatg
    42721 gtcaaagtag tctgaaagca taatggcatt gtcaaggcct tcagcaccgc accaacgttt
    42781 ttgagcaaaa ggtaggtcca ttgaaacagt caatacgacc gtgttgtcca gtccagccaa
    42841 ttcttcatta aaacgacgtg tttgagttga gcagatgcct gtatcgatag aaggaacgac
    42901 actcaagact tttttcttgc catcaaaatc agccagagat tttttagaaa gatctgttgt
    42961 agtaagagaa aaatcaagcg ccttgtcgcc aacttgtagt tgtttacctg taaagctcac
    43021 aggatttccg agaaaagtta ccataggata ctccaatctt ttttcttcca ttgtatctga
    43081 aacagtcaga attttccaat gatttgaccg gaaatgtggg catagaaaaa acgccagctc
    43141 acatgagaat gacgtttttc agaggcttat tttgccaatc cttcagcaat cttgtcaagg
    43201 ttgtatttca tcatgctgta gtagctgtcg ccttctttac cttgttctgc gatagagtca
    43261 gtaaagattt gtgcgtagat tgggatgttt gtgtcttgag aaacagtttt cattggacgg
    43321 tcatccacac ttgattctac aaagagtgat ggaacttttg tttggcgaag tttttcaacc
    43381 aaggtcttga tttgttcagg agttccttct tcttcagtat tgatttccca gatgtaggca
    43441 cttgggacac cataggcttt agagaagtat ttgaatgctc cttcgctggt tacaatgagt
    43501 ttcttttcag cagggatctt attaaattta tccttacttt ctttatcaag tttgtctaac
    43561 ttatcagtat attctttgag atttttttca tagaattctt tattgttagg gtctttggcg
    43621 ctcaattgtt tggcgatatt tttagcaaaa ataataccgt tttcaaggtt aagccaagcg
    43681 tgtgggtctt cttttccttt ttcattttga ccttcaaggt agataacatc aacgccgtcg
    43741 ctgactgcga agtagtcttt gttttcagtt ttcttggcat tttctaccaa ttttgtaaac
    43801 caagcattgc cacctgtttc aaggttgata ccgttataga aaatcaaatc agcctcagaa
    43861 gttttcttaa cgtcttcagg aagtggttcg tattcgtgtg ggtcttgccc aatcggaacg
    43921 atactatgaa ggtcaatttt gtcaccagca atatttttag taatatcagc gatgattgag
    43981 tttgtagcaa caacttttag tttttgacca gaagttgtat ctttttttcc gctagcacat
    44041 gctacaagaa tgattgcaga aagaaagaga acgagtaatg tacctaattt tttcattaga
    44101 tcctccaatt tattagggct ttgcccctta ttttaacaaa tgtttatttt tcagtttcaa
    44161 atatcgttgt ttgggagcga taaagaagct aatgagaaag aaactagcag ctgtaagcac
    44221 gatactagaa cctgccgcaa cattaaaact atagccaata aagagtccca aaactgaagc
    44281 agtagctccg aaggttgagg aaaggaaaat catacttttc agactattag catacagata
    44341 agcagttgca gctggggtaa tcagcatggc tacaatcagg atagttccga cactttgcat
    44401 ggctgtcaca gacacgagag tcaggagtac catgagaagg tagtgataga aattgacagg
    44461 cattcccatg gctttagcca agagttcatc aaaggaagtt atcaagagtt gcttgaagaa
    44521 aatccaaatt aacaagagga tggctgcccc cacacccata gtaataaaca tatccgtatc
    44581 ttggacggcc aggatattac caaaaaggat atggaaaagg tcagttgaac ttttagcgac
    44641 accaatcaag atgataccga gggctaagaa agaagaaaag gtaatgccga tggcggtatc
    44701 gcttttgata atcgagtttc ctttgatgta ggtaatgatg atggcagcta gcaatccaaa
    44761 gacaatggct ccgataaaga agtcaaggcc caagatgaag gagagggcta cacctggtaa
    44821 gacagcatgt gaaatggcat ctcccatgag tgacatcccg cgtagaataa tgaaacatcc
    44881 cacggctcca gctacaatcc cgacgacaat agctgttatc aaggcatttt gtaggaaatg
    44941 gaatttttgc aatccatcga taaattctgc aatcataggt cacctccatt gaaaaagagt
    45001 tgattaccgt aagcttcttt tagattggtt tcggtaaaag tttcttttgt tggaccaaag
    45061 gcaatcactt ctcgattgac aagtaagact tgatcgaagt agtggggaat cttgctgagg
    45121 tcgtggtgaa cgatgagaac cgtcttccca gcttttttca aatctctcag cgtattcatg
    45181 atgatttcct cactgacaga gtcaatccca gcaaagggtt catccaagag gatatagtcg
    45241 gcttcctgca ccaaacatct ggcaatcaag acccgctgga attgacctcc agacagttga
    45301 ctaatttgac gttcagcgta gtcagctagg ccgacgattt caagggcctc ttgcactttc
    45361 ttccaatgtt tagcctttaa acttcgaaag agaggaatag agggaaatag tcctaacgag
    45421 acgcattcct tgaccttgat gggaaagttg tagtcgatat tgattttttg ttcgacatag
    45481 gcaattcggt gtaaggattt tttaacttcc ttgtcatcga gaaatgcctg accttgatgt
    45541 gggataattc ccaacatacc ttttaatagt gttgatttcc cagcgccgtt tggaccaatg
    45601 atgccggtaa ttgttggtcc atggagcact agtgaaatat ccttaagtgc caacgtttct
    45661 ttgtaggaga cactgaggtt ttcgatacgt atcataaact tgtattcctc ctgtctctta
    45721 atatacatta aaaaaaaaat taagtcaagt taatttttga aaaaattaaa ataataactg
    45781 aaaaatagat tctaaaaata actttcagga taaatttcta aattataaaa cgcatagtat
    45841 caagtgtaaa aaacttggaa ttatgcgttt tatcatggaa aggtttttta taatagctaa
    45901 aaaataatag ttaaatggtt attaattgca ttccctagtg atttttgtta agataaaggc
    45961 aaatacaaat gaaagcgaga acaagatgac acgttatcaa gatgattttt atgatgctat
    46021 caatggagaa tggcaacaga cagctgaaat cccagcagat aagtctcaaa caggaggttt
    46081 tgttgattta gaccaggaaa ttgaagacct gatgctggcg acaacagaca agtggttagc
    46141 aggtgaagaa gtgcctgagg atgctatctt ggaaaacttt gtcaaatacc accgcctagt
    46201 tcgtgatttt gacaagagag aagctgacgg tatcacacct gtcttaccac tccttaaaga
    46261 attccaagaa ttggaaactt ttgcggattt tacagctaaa ctagcagagt ttgagcttgc
    46321 aggaaaacca aacttccttc cttttggtgt atcgccagac tttatggatg ctagaatcaa
    46381 tgttctatgg gctagcgctc caagcacaat cttgccagat acgacctact atgcagaaga
    46441 acatcctcag cgcgaagagc tcttgactct ttggaaagaa agtagcgcaa atctcctcaa
    46501 ggcttatgat ttctctgatg aagaaattga agacttgcta gaaaaaagac ttgaattgga
    46561 ccgccgagtt gcggcagtgg tgctctctaa tgaagaacgt tcagaatatg ctaaactcta
    46621 tcatccatat tcttacgaag atttcaagaa attcgcgcct gccctacctt tggatgactt
    46681 cttcaaagca gttattgggc aattaccaga caaggttatt gtagacgagg aacgtttctg
    46741 gcaagcagca gagcaatttt acagtgagga agcctggtct ctccttaaag caaccttgat
    46801 tttgagtgtt gtcaatcttt caaccagcta tttaacagag gatatccgtg ttttgtctgg
    46861 tgcctacagc cgtgcccttt ctggagttcc agaggcaaaa gataaggtca aagcagctta
    46921 tcatctagca caggaacctt tcaagcaagc cctggggctt tggtacgccc gtgagaagtt
    46981 ctctccagaa gccaaggcgg atgtggagaa aaaagtggca accatgattg atgtctataa
    47041 ggagcgtctg cttaagaatg actggctcac tccagaaacc tgtaaacagg ctatcgtgaa
    47101 gctcaatgtg atcaaacctt atattggcta tccagaagaa ttgcctgcac gttacaagga
    47161 taaggtagtg aatgaaactg ccagtctttt tgagaatgct ctagcctttg cgcgtgtgga
    47221 aatcaagcac agttggagta agtggaacca gcctgtagac tataaggaat ggggcatgcc
    47281 tgctcatatg gtcaatgcct actacaatcc tcagaagaac ctgattgtct ttccagcggc
    47341 cattttacag gcgcctttct atgacttgca tcagtcatct tctgctaact acggtggtat
    47401 tggggcagtg attgcccatg aaatttccca cgcctttgat actaacgggg cttcctttga
    47461 cgaaaatggt agcctcaagg attggtggac agagagcgac tatgctgcct tcaaggagaa
    47521 aacacaaaaa gtcattgacc aatttgatgg acaggattct tatggagcaa ccattaacgg
    47581 taaattgact gtatcagaaa acgtggctga cttgggagga atcgcagcag ctcttgaagc
    47641 agctaagaga gaagcagact tctcagcaga agagttcttc tacaacttcg gtcgcatctg
    47701 gcgcatgaaa ggtcgaccag aatttatgaa acttttggct agcgtcgatg tgcacgcacc
    47761 agccaaactc cgtgtcaatg tgcaagtacc aaacttcgac gatttcttta caacctatga
    47821 tgtcaaagaa ggagacggaa tgtggcgttc accagaggag cgcgtgatta tttggtaatg
    47881 caaaaaatct agtcatcaga aaaaaggcat ccaatcacgt gtgaaaacct tgataggatg
    47941 ccttttataa tagaaagatt tgctggatag tttacttaaa tttcgattgt catcatcttg
    48001 ctaaaaaaag ggattgaagg ctttttcgtg agcgatagta gtagctggac catgtcctgg
    48061 atagacatcg tagtttggta gggtgaagag ttgggtctgg atactatgaa ggagttgctc
    48121 catgctacca gtcggaaggt cggtccgtcc gatagtttcg cggaatagag catctcccgt
    48181 caagactagg taagcatcag gaaagactag ggaaacacca ccgatagagt gccctggggt
    48241 cggtagaacc ttaaaacgaa attcctctag ttggtattct tcgtgaaaga caaaggtgtg
    48301 ttctgcaggt tttgtgacca catctgccat atcatcgtgg cgagggagac cggagagatt
    48361 atcgacagga gtgtagagcc agctggcttc gctctctgcg atatagacag gaggattgcc
    48421 aaacgtctcg cgaaccaagt ccagactcat gatatggtca taatgggcgt gggtcaagag
    48481 aatagcacag atcggtttgt tgatcttctc gattgtctga cgaatggctt cccaatgact
    48541 accaggatcg acgacgatga ggtgcttttc gccttctaga taataggtat tttcataggc
    48601 aacaggattc acggttttat ggattttcat actagctcca atctcaaaga atgtactaat
    48661 actcttcgaa aatctcttca aaccgcgtca actttatctg caacctcaaa gcagtgcttt
    48721 gaacagcctg cggctagctt tctagtttgc tctttgattt tcattgagta taaattaagt
    48781 atagcacagt tagggagaat aggtaaggat ttaaaatgaa cttaaaaatc agcatgacta
    48841 gatgaagaca ggctggttat caatttatca atattggaaa ggtgtcaatt gcccttcata
    48901 ggttgcataa actccgtcat tagcttgttt gatagacgtg atgtttagag tgctaccgta
    48961 gttggcttct catttgtcgc tatattcact ataagttatc ctactgggca ggttctggtc
    49021 gttagcatag tattgaacga gttttttata gctttgctgg ctgaatagga acaggcagca
    49081 aactcctaac attaggagag caaagataga aatagcagtt tttttcatag gtattctcct
    49141 tataaactaa aggatagaag aaagtatctt gaggattttt atactcttca aaaatctctt
    49201 caaaccgcgt caactttatc tgcaacttca aagcggtgct ttgagtaacc tgcggctagt
    49261 ttcctagttt gctctttgat tttcatttag tattaattcc aaaatccagt caaggtacca
    49321 gtgtatgtgc tacgagttcc accgtttatc tttcgtattt tcaatggaat tggggaagtc
    49381 agaaagtctt tgggttacaa tatggtttcc gttcttctat ctctccttct atcacctaag
    49441 tttaaaatct aacttcgtag tatattatgg gctaataatt cagaattatc aagcaatttt
    49501 gaattatttt tttcttattc tgttccaatc tagttttcat attcttaaaa aagtgataaa
    49561 atggtaggaa gaattggaga gtagagatgc cgaaagaagt gaatttaaca ggcgaagaag
    49621 ttgtcgcttt aaccaaagaa tatttaacgg aagaggatgt tcattttgtc cataaggcct
    49681 tggtctatgc tgttgaatgc cacagtggtc aatatcgcaa atcaggcgag ccttatatca
    49741 ttcaccctat ccaagtggca ggtattttag ctaagctaaa gctggatgct gtaacagtag
    49801 cttgtggatt cttgcatgat gtggtggaag atacagatgc gactttggac gatttggaaa
    49861 gagagtttgg tcctgatgtg cggatgattg ttgacggagt taccaagctt ggcaaggtcg
    49921 agtacaaatc gatcgaggag caattagcgg aaaatcatcg caagatgctc atggccatgt
    49981 ctgaggacat ccgcgttatt ttggtcaaac tgtctgaccg cttgcacaat atgcggaccc
    50041 tgaaacatct tcgaaaagac aagcaggagc gtatttccaa agaaaccatg gaaatctatg
    50101 ccccacttgc ccatcgtttg gggatttcca gtgtcaaatg ggaattagaa gacttgtctt
    50161 tccgttatct caatccaacg gagttttaca agattaccca tatgatgaag gaaaagcgca
    50221 gggagcgtga ggccttggtg gatgaggtag tcacaaaatt agaggagtat acgacagaac
    50281 gtcacttgaa agggaagatt tatggtcgtc ccaagcatat ttactcaatt ttccgcaaaa
    50341 tgcaggacaa gagaaaacgg tttgaggaaa tctatgatct gattgctatt cgttgtattt
    50401 tagataccca aagtgatgtt tatgccatgc ttggttacgt gcatgaattt tggaaaccga
    50461 tgccaggtcg cttcaaagac tatattgcca accgcaaggc caatggttat cagtctatcc
    50521 atacgactgt ttatggacca aaagggccga ttgaattcca gattcgaacc aaggaaatgc
    50581 acgaggtggc tgagtacggg gttgcggctc actgggctta taagaaaggt ataaaggggc
    50641 aagttaacag caaggaatca gctattggaa tgaactggat caaggagatg atggagctcc
    50701 aagaccaggc tgatgatgct aaggaatttg tggactctgt taaggaaaac tatttggctg
    50761 aggagattta cgtttttacc ccagatggag ctgtccgttc ccttcccaaa gattcaggac
    50821 cgattgattt tgcctacgaa atccatacca aggtcggtga aaaagcaact ggtgccaagg
    50881 tcaatggccg catggttcca ctgacaacca agttaaagac aggggatcag gttgaaatta
    50941 tcgccaaccc gaactccttt ggacctagcc gtgactggct caatatggtc aagactagca
    51001 aggcgcgcaa taagattcgc cagttcttta aaaaccaaga taaggaattg tctgtcaaca
    51061 agggtcgtga gatgctgatg gctcagttcc aagaaaatgg ctatgtggca aataaattta
    51121 tggacaagcg ccacatggat caagttctgc aaaagaccag ttacaagaca gaagactccc
    51181 tctttgcggc cattggtttt ggggaaatcg gtgcgattac cgtctttaac cgtctgactg
    51241 aaaaggaacg ccgtgaggaa gagcgtgcca aggccaaggc tgaggcagag gagcttgtca
    51301 aaggtggcga ggtcaaggtt gaaaataaag aaactctcaa ggtcaagcat gaggggggag
    51361 tggttattga aggtgcttct ggtctcctag tgcggattgc taagtgttgt aaccccgtgc
    51421 ctggtgacga tattgttggc tacattacca agggtcgtgg tgtggctatt caccgtgtgg
    51481 actgtatgaa cctgcgtgcc caagaaaact acgagcaacg tctccttgat gtggaatggg
    51541 aagaccagta ctctagctca aataaggagt atatggccca tatcgatatc tacggtctca
    51601 accgtacagg actgttgaac gatgtactgc aagttctttc aaatacaacc aagaatattt
    51661 caacggtcaa tgcccaacca accaaggata tgaagtttgc taatatccat gtgtccttcg
    51721 gtattgccaa cctctctaca ctgaccacgg ttgtcgataa aattaagagt gtgccagaag
    51781 tttactctgt caaacggacc aacggctagt tgtcctagct cttactagaa aggctattat
    51841 gaaaatcatt atccaacggg ttaaaaaagc ccaagtgagt atagaaggcc agattcaggg
    51901 aaaaatcaat cagggacttt tattgctggt tggtgttgga ccagaggacc aagaggaaga
    51961 tttggactat gctgtgagaa aactggtcaa tatgcggatt ttttcagacg cagaaggcaa
    52021 gatgaacctg tctgtcaaag atattgaagg agaaatcctc tctatttctc agtttaccct
    52081 ctttgcggat actaagaaag gcaatcgtcc agcctttaca ggggcagcta aacctgatat
    52141 ggcatcagac ttctatgatg ctttcaatca aaaattagcg caagaagtgc ccgttcagac
    52201 aggtatcttt ggagcagata tgcaggttga gctggttaat aacggacctg ttaccattat
    52261 cctagatact aaaaagagat aagaaagacc aagcccagtc ggcttggtct ttctcatcga
    52321 tcataaaata ctccaaaaag aaatcggttc ttgatatgct tgggggaagt ctcttttcag
    52381 gctttggcag atgcgatagg aagggatgag atgtcctagg atgaggagag ttccctgaag
    52441 gaaaagtgga atatcactta aagacaccaa ggagagaatc atcaggctat cccataggag
    52501 aatttgtaaa gcaaaacgtc agaatctcgg tctaatctgg gaatagagtt gactaaagtg
    52561 gtcacaaagt tggtttgaaa gataggtcaa tagcacagga tgaaagaaaa tgagccaacc
    52621 actataaatc aaaatccagt cccaagtaag cccccctttt aaatgtcaaa aatgccagca
    52681 tgattccatc aatgacaagg agttgaatgg ttttgatgaa gatgattttt ttcatgctaa
    52741 aacctccttt tttcatctgt tagtgacgta atgctttata taattattat acagtattca
    52801 gtcgggatat gaaaacgttt actatcgttt ttgatataaa aaatagataa gaaaaccctg
    52861 aaaataaaac tttcagggtt ggtaggggac ttgagaaatt agttgatttt ctcgacatag
    52921 agttgttttg caatgtccat attcacttgt aaatcctcgt cgttactgag gatagtgaag
    52981 gtattgctga agccatcaaa ctgcttgact tggagctggt caccgatgtg aagtgagtgc
    53041 ttgtccagat aatggagaat gtcaaaacta tcgtgcaccc gagtcaggcg gtaggcgcca
    53101 gcttccttga tatcagctag tgggaggtta ttgatttcaa cgaggagttc tcccttggca
    53161 ggaatagttc ctccgtgggg gcaggtttta gggaaaccta gcagtttatc tagtctttcc
    53221 acgaacaggt cagagacagt gtgttccaag acctcagctt cctcgtgaat ctggtcactt
    53281 gtatagtcta aatgatgaac tagaaaaact tcaatcaagc ggtgcttacg atagagctca
    53341 gagaccagtt tgagaccgag gtcagtcagt agatagccac attccttgtc ctttaggatg
    53401 agattttcac ttttcattcg tttgatcatt tcagttacgg cagggggaga gacttgcatg
    53461 cgagccgcaa tttccttgtt ggtaatctta tgcaggtcta tgccaatttc ataaatacat
    53521 tttagatagt cttctttgtt tggggtcatt cgtttcctct tgtctaatat ctttatctag
    53581 tatatcaaaa aaagctagat ttctctagct gtgacttttt atgttatact tattgcaaga
    53641 gaaaaaacga ggagatggat atgacgaaaa tagctcttct ttcagatatt catggaaata
    53701 ccaccgcctt ggaggctgtt ttggcagatg ctcagcagct aggagtggat gaatactggc
    53761 ttttgggaga tattctcatg ccagggacag gacgtagaag gattttggac ttgttggatc
    53821 aactaccgat tacggctaga gttttgggaa actgggaaga cagtctttgg catggtgtcc
    53881 gtaaggaatt ggacagtact cgccccagtc aacgctatct cttgcgccag tgccagtatg
    53941 ttttagagga aatttcccta gaagaaattg aagtgctcca caatcaacct cttcaaattc
    54001 atcgtcagtt tggggatttg acggtgggaa ttagccatca tctgcctgat aagaactggg
    54061 ggcgagagtt gattcatact ggcaaacaag aggagtttga ccgcttggtg actcatcctc
    54121 cctgtgatat tgctgtttat ggacatattc accagcagtt gcttcgttac gggactggtg
    54181 ggcaattgat tgtcaatccg ggttcgattg gccaaccttt ctttttggac gctcagctac
    54241 ggaaggactt gcgggcccag tatatgattt tggagtttga tgacaagggt ctggtagata
    54301 tggacttccg acgggtagac tacgatgtgg cagcggaatt gcagctggct aaagacctga
    54361 gacttcccta ttttgaggtt tactatgaaa gtctggtcaa tgggatccac catactcatc
    54421 atcaggaatt tttgagagaa ttggctcaga aagagggctg cgatcgggag ttagacgact
    54481 ggttgaaaag tggtaacgat tgacattaca actaaaaagg gtataataat agaaaaagaa
    54541 gagtagaggc agggtagcct cgtaaaaagg agaagaggat gcaaattcca agtagattta
    54601 ccattgcgac tcatatgctg ataatcattg ccctcgaggg gaaggaaagc aaggtgacca
    54661 gtgattttct ggctgctagt gtcggggtca atcctgtcat tatcagaaat atcttgtccc
    54721 agttgaagaa ggcagagctg atttcagtag cgcgtggaac gggcggaaca gagattgtca
    54781 aggaccttaa ggatattagt cttttagatg tttatcaggc ggtcgaatgt cttggtaaga
    54841 caggtcaact cttcagtttc catgacaatc cgaatccaaa ttgccctgta ggagctcata
    54901 ttcatgatgt tttggatcaa aaattggaga gaattcagtt gactatggag gcagaacttg
    54961 gtcaaaccag tctagaaaaa gtcgtggccg atgcagagag tcagatgaag gattaagagg
    55021 gataaatgcc ctcttttttc tttggataat tatgataaaa tgtagctatt aaaggagtaa
    55081 gaaatgtatc tcgttatagg aattgtatta gcctttatag tgtcattttg gaaggataat
    55141 agaagtttgt ggaatccagt attattttta ttatccctca tttcaagtta tttttatcta
    55201 tcctaccttt tttataaaaa tggatacgag aatgttcagt tagcttttta tgtatttacc
    55261 tttgtcttac ttcctttttt gctttttcta agtggcatct ttttaatcta taatggggtc
    55321 attttgttga agtgagaggg acgttccaag ccccattatc tctcaatgct ctttgatttt
    55381 tattgactat tagattctat ttaaaaagac ttagaatgca catgaaagta tgtattctag
    55441 gtcttttgtt ttgtatacat ttaattttca agagtattat ctatttttcc atgcctggta
    55501 acgagtatca atcaataact gggtaaggcg acccaaggtt tctctttctt ctggagtgag
    55561 ggattgagct tggacaaaga gatgatgaat gtattcaagc gcctttaagg aagccaagtc
    55621 gttgatagag ctaataccag catcaagaat agtgccaaag aagaggtcga agtagagctg
    55681 aagttcttcg tcagggactg tggccaccca ttctttaaag gttgtgtcta cttgctggct
    55741 atcactgttg gtcttatcca gttggacgaa gtgcttgtcc tcaatctgcc aactaaaggt
    55801 atcgtgctgg gcgatgccac ccagggcagt actctgaacg atgatttggt gagcaggaat
    55861 ttccagcatc ataccgataa tggaaccttg tggaatgaat atcttgcttc tatccattat
    55921 cctttgataa cccgcagtct gtgtcaattc ttgatggaga ccaggtgcat caaatgtata
    55981 aactgctgtg atctgatttt gcaaactttg ctcaatttgg ctagcagcat agatagcgag
    56041 atttcctccc ttggaatgcc cagccagaat aaccttttgc ttaggatgat gggcaaaaaa
    56101 gttctttaaa tagcgaaggg cgtgcttttg agcaggaatt tccttcatat aggtcaggtg
    56161 gaaatcttcc ttccagccaa tgatactgtc atctgtccca cgaaagacaa tcagataggt
    56221 atcgaggctg acacgataag tcatagccgc aaattgcttt tgcagttcag ggtcgatgtc
    56281 gttgataaaa tgggagagtt tgcaattttt gaagcgcttg tgttgagcca attcatctaa
    56341 taattgaagg cgatttttgc tagtaagcat ggtgggctct cttggaacct gaggtgctag
    56401 ctctaaaaga cgttgaggaa gtgtggagac cagattatca aaggagaggt aggtgatttc
    56461 tattaaggtt agaatgtcta actcattcaa gggaaggtcg taataagaat catatgcgac
    56521 atctttcaga tagtcaaaaa tattggccat aagagctcct ttctttttat ttatcttagc
    56581 aaatttccct caaattagct agtagcattg cctgtttgta ctggctaaaa acaggctatt
    56641 tcaaattcag tttcagacca tctggcatgg aaaaatctgt tataataatg gaaaaggaga
    56701 agcgcatgca caagatttta ttagtggaag atgatcaggt cattcgtcaa caggttggga
    56761 aaatgctctc tgaatgggga tttgaagtgg tcctggtaga agactttatg gaagttttga
    56821 gtctatttgt tcagtcggaa cctcatctgg tcctcatgga tattggtttg cccttgttta
    56881 atggttatca ctggtgtcag gaaatccgca agatttccaa ggtacctatc atgtttcttt
    56941 cttcgagaga ccaggctatg gatattgtca tggcaatcaa tatgggggcg gatgactttg
    57001 tgaccaagcc ttttgaccag caggttcttt tagctaaggt tcagggcttg ttgcgtcgtt
    57061 cctatgagtt tgggcgtgat gagagtttgc tggaatatgc tggtgttatc ctcaatacca
    57121 aatccatgga tttacattat caagggcaag tcttgaattt gaccaagaac gaattccaga
    57181 ttttacgcgt gttatttgag catgcaggca acatcgtagc acgtgacgac ctgatgcgag
    57241 aactttggaa cagtgacttt ttcattgatg ataataccct ctctgtcaat gtggctcgtt
    57301 tgcgtaaaaa gttggaggag cagggattgg taggatttat cgagaccaag aaaggaatag
    57361 ggtacggatt gaagcatgct tgattggaaa caattttttc tagcctatct gcgctcccgt
    57421 agtcgtcttt ttgtctatct gctttctttg gcatttctgg tcttactctt tcagttttta
    57481 tttgccagtc taggaattta cttcctctac tttttcctct tgtgttgctt tgtaaccatc
    57541 ttatttttca cttgggacat attggtggaa atgcaggttt atcgccagga acttctctat
    57601 ggagagaggg aagccaagtc tcctttggaa atagctttag cagaaaaatt agaagcgcgt
    57661 gagatggaac tctatcagca gaggtcaaaa gcagaaagaa aactgacgga tttgctggat
    57721 tactatacct tgtgggtcca tcagataaag acccccattg cagccagtca actcttagtt
    57781 gcagaagtgg tcgaccgcca actgaagcag cagctagaac aggaaatttt caaaatcgac
    57841 tcctatacca acctagtttt acagtacctg cgtttagaaa gtttccatga tgatttgctc
    57901 ttaaagcagg ttcaaattga ggacttggtc aaggaaataa ttcgtaaata tgctcttttc
    57961 tttattcaaa aaggcttaaa tgtcaatcta catgaccttg ataaagaaat cgtgacggat
    58021 aaaaagtggc tgctagtggt tattgagcaa atcatctcaa acagtctcaa gtacaccaag
    58081 gaaggtggtc tggagattta tatggatgac caagagcttt gtatcaaaga tacgggaatc
    58141 gggataaaaa acagtgatgt cctccgagta tttgaacgtg gcttttcagg atacaatggc
    58201 cgtttgaccc agcagtcctc tggacttggc ctttatctat ctaagaaaat ttctgaagaa
    58261 ctggggcacc agattcgtat cgagtctgag gtcggaaaag gaacgacagt gcggattcag
    58321 tttgctcaag tgaacttagt ccttgagtaa aaatagatga gaaaaagcct ccagattgga
    58381 ggcttttatg ctagactctt atttctcaac gcgtgatttg ttggcgatat cagctaggat
    58441 agcttgaaca aaattatcaa ctgagacagt ttgtgtttct ttttggccgt agcgacgaac
    58501 gttgactgtt tcgtcttcca tttctttgtc tccaacaatt aattggtaag gaatcttgct
    58561 ggtttgtgaa gcacggatct tgaactgcat tttttcattg cgctcatcta catctgcacg
    58621 gacaccgcgg tcacggagtt tcttggccac ttcccaagcg tagtccacgt gtttttcgtt
    58681 agatactggg atgagggtta cttggtgtgg tgccagccat gttgggaagg cccccttgta
    58741 gttctcaatc aagatagctg tgaagcgttc catagttgag ataactccac ggtggatcat
    58801 gactggacgg tgatcttcgc catcagctcc gatgtatttg aggtcgaagc gttctggcaa
    58861 caagaaatca agttggatag tagaaagggt ttcttctttt ccaagggcag ttttaatctg
    58921 gatatccaat tttggtccgt agaaggctgc ttcaccttcg gcttcaaagt agtccacgcc
    58981 catttcatca agagctgcac gaagcatggt ttgggcattt tcccacatct catcgttatc
    59041 aaagtactta tgagtatctt gagggtcacg aagagagagg cggaagcggt agtcagtcaa
    59101 gttgaagtct tcataaacat cgataatcaa ctgaagggca cgttggaatt cttcttggat
    59161 ttgttctgga gtaacgaata ggtgaccgtc gttgagtgac atttcacgta cacgttgaag
    59221 gccagtgagg gcaccagatt tttcgtaacg gtgcatcata ccgatttcag cgatacggat
    59281 tggcaattca cggtaagagt gaacatggtg tttaaaaact tggatgtggt gcggacagtt
    59341 cattggacga aggacaaatt cttccccgtc acccatgtcc atggttggga acatgtcttc
    59401 ttggtaatga tcccagtgac cagaagtctt gtaaagctca acagaagcaa gtggtggagt
    59461 gtagacgtgt tggtagccag aagccaactc tttgtttacg atgtagcgtt ccaattcacg
    59521 acggatagtc gcaccatttg gcaaccagaa tggcaaacct tgtcccactt cttgtgaaat
    59581 cataaagagg tcaagctctt taccaagttt acggtggtca cgttccttag cttcttcacg
    59641 catttgaagg tagtttttca agtctttctt gtcaaaccaa gctgtaccgt agatacgttg
    59701 catcatagcg ttgtcgctgt ttccacgcca gtacgcacca gctacatgga gaaggtggaa
    59761 gatttggata cgacctgttg atggaacgtg aggtccacgg cagaggtcta catattcacc
    59821 ctgacgatag atagtcaaac cgccttcgtc ttctgagtgt tcttcaatca attccaactt
    59881 gtaagggtca tttttgaaga tttcacgtgc ctcgtcttta gtcacttctt cacgaataga
    59941 tgggaagttt tctttgacga ttttttgcat ttcttcttcg atacgaggaa ggtcttcgtt
    60001 agagatttga ccagctgtgt tgtcagtatc gtagtagaaa ccatcttcga tggctggacc
    60061 aactcccaag tgaatgtctg ggaaaagacg acgagctgct tgggcgaaca agtgagctgc
    60121 tgagtgacgc aagattggaa gggcatcttc gtgatcaggt gtcacaattt cgatgcttcc
    60181 atcttcagtg atagcgcgag tagtgtcgat gagtttgccg ttgaatttac cagccaaggc
    60241 ttttttagct agggaattgc tgatagattg ggcaatttca aaagttgtta cgccagattc
    60301 gaattcacga acagcgccat ctgggaaagt aatgttaatc atgatgttct ccttttttat
    60361 tatttcatag tttctaagca ttgatttttt atcagcagac tacttccgag tcctaacgtt
    60421 caattgcttt tctttatcag agaggaagtt tttcatttcc gtcagaatgt ctatatccaa
    60481 acgttgagaa agttctaata gccaccagat attttctgaa agtttttgtt ccagtgtgta
    60541 gggtgtttca tcatagtagc gtccttgctt tgtcatcacc agtcattgga aatttccaat
    60601 atcattagat aaagccaaga ggtcttcttc taccgtccac ttgaaatcat gatgcttaac
    60661 ttccagttcg tgataagctt gtcggattgc ccaactgcgc tccactaatt cttgcaactc
    60721 cataattagt atcctaataa aaaaatgcga catcctcgaa aggacgtcgc aacgtggttc
    60781 caccttcatt tatgtacctc aaaagagagg gacacctctg atcggctcta acgtggccac
    60841 cgttttatgt tttcataaaa aactcaagag tagtatcagt ttagacttcc tatgcgtttc
    60901 cagcaaccac gcactctcta gtagaaaggg actaagtgac ttgtctctat ggattattat
    60961 agcatgattg tgagattttt caaggatttt gtgaaagttt tttgtttttc attttttgta
    61021 gaatatgctt gatgctaata ccagaatcca tgccgaccag agcaactgtt tcatagatta
    61081 gcaagtaaat tagtataaac tcaccaaagg ttatgatagt aaagtaaaag agcagtccgt
    61141 taccaactaa ccatcaaagt gagaaacgga aacggtagct atagagtaac gatataatca
    61201 agattaccag aggtgtaaag aagagaagat tgtttatata gagttccttg agtgtaaggg
    61261 ggtcagaaac aactagtaag aacaactcat aaagtggcca ataaaagaat aatatgacca
    61321 gatagtaggg gagatgggaa tagaatctac gcatgataat cacctcgttt taaaaaaaca
    61381 gacaacttag atctcttgct atgattatta tagcatgttt aaaacaagaa agtaaatttg
    61441 ttgacaaaga agatttttct tttcactaga atcatagtta gttttcttgg tagaaatatt
    61501 ttgataataa ttaagcaaga ctattgacat taattattaa gagtgtcaca attgttgtaa
    61561 ctaaaataat tacaacaaga gaggtataaa aaatgaaact agcagttatt gcagcaaatg
    61621 gacaagcggg gaaagcaatt gttgaagaag cagtcaaacg aggacatgaa gtaacggcta
    61681 ttgttcgttc tgaaaacaaa agtcaagctg agtctattat taaaaaagat ttatttgagt
    61741 tgacaaaaga tgatcttact ggatttgatg ctgttatttc agcgtttggt gcttatacac
    61801 cagatacgct accgctccac agcaaatcaa tcgaactatt taatcagctt cttgctggaa
    61861 cacagacacg ttttctagtt gtagggggag caggaagtct ttatatagat gaaacaaaaa
    61921 cgacacgact tttagatacg tctgattttc ctgaagaatt taagtcacta gcaaaagctc
    61981 aagcggatga attggatctt cttcgtacaa aaaataatct caactggact tttgtcagtc
    62041 ctgctgtaga ttttattccc gatggtgaaa aaacaggaaa ctacattcta gcaggagaaa
    62101 tttttactac taatggaaaa ggaataagcc agattagtta tgcagattat gctattggat
    62161 tggtagatga acttgaaaaa ggtcatcata taaaagaacg tatctcttta cttgaaaaat
    62221 aataactatg agagtattaa tgtttgacag aaaatttaaa tcttggtaaa ataaggattg
    62281 tcgtaaagac aaataacttc ttcttggtta caggcatgcc aacctgtcac tcggatgaag
    62341 ccaaataaaa aggagaaaca tcatggcaat ctcaaaagag aaaaaaaatg aaatcattgc
    62401 acaatatgca cgtcacgaag gtgatacagg ttcagtagag gttcaagttg ctgtccttac
    62461 ttgggaaatc aatcacctta acgaacacat caaacaacat aaaaaagacc acgctactta
    62521 ccgtggattg atgaaaaaaa tcggtcgccg tcgtaacttg cttgcatact tgcgtaaaaa
    62581 cgacgttaac cgttaccgtg agttgatcaa ctctctagga cttcgtcgct aattcaagat
    62641 acaaatgctg tcaaaagcac aaagcaaaaa taggaaaatt gacgaagaaa cttcagtttc
    62701 taggagattt tatctttttt gccaagtgct taggccgtgt tcaattgagc atatcttgat
    62761 aataaagcta ctctaaattg ggtagctttt ttgcgttgtc accttacctc gatatactca
    62821 agtataatct ttggttacgg ttcctagcac tgtaaggtaa aataaaccag cttttctccc
    62881 ttcggggaga tttttttgtt tcactgaaag tttcgtacga tcttcgcctc gtcgcctagt
    62941 ctataaacga tttatctagc aagaatcatg atgctaaggg cgtcaaaaat ccgtatgaaa
    63001 atagggaaat gacacagtgt tcgatgagca caaggagttt catctttttc actaggattt
    63061 tagcccgggc tcaaatcagc tctctgactt cagagggctt ttttattgag gttttatcgg
    63121 taacaatttt ggagactaca aaaatctcaa acggtatcag ctaattacac cataaaggtg
    63181 cgcagttact cggcttttca agccgagtag ctgtatgcaa gccccctaga agagcccact
    63241 ctttacaaag taaagtatag tatgttatac tttacatgga agtagtcact gaattccagt
    63301 tagaaattac tttgtaacta cgttttgagg aggagtaaaa tgctttccta cgttcgacat
    63361 tacccactag cgatagctaa attaatgtgt ctgtgctctc ctaaaatctg ctgatttatt
    63421 actgactaat acaggagttt tttttatggg acagacaatc atatctgcta ttggtgttta
    63481 tatttccacc agtatcgatt atttaattat tttaattatt ttatttgcac agctatcaca
    63541 gaataaacag aaatggcata tttatgcggg gcaatatcta ggcacaggct tacttgtagg
    63601 ggcgagttta gttgctgctt atgtcgttaa tttcgtgcct gaagaatgga tggttggatt
    63661 gcttggttta atccctatct atttagggat tcgctttgca attgttggag aagatgcgga
    63721 agaagaagag gaagaaatta ttgaaagatt agaacaaagc aaggcaaatc aactgttttg
    63781 gacagttaca ttgctgacaa ttgcgtctgg cggagataat ttaggtatct atatacctta
    63841 ttttgcttcg ttagattggt cacagaccct cgtggccttg cttgtgtttg taatcggcat
    63901 aattatcttt tgcgagatta gtcgggtgtt atcctctatt ccgttaatat tcgagacaat
    63961 tgaaaaatac gagcgaatca ttgtgccctt agtattcatt ctacttggac tatacatcat
    64021 gtatgaaaat ggcacgatag agacttttct gatcgtgtag atttttttgt ttcactagga
    64081 ttttagtccg agctcaaatc agctctctga ttttcagagg gctttttcat tgagtataaa
    64141 tggacaatct taaagttact ctattgtaga gtggcttttg atttgattag gagattctta
    64201 taaatatagt aaaatgaaat aagaatagga caaatcgatc aggacagtca aatcgatttc
    64261 taaaaatgtt ttagaagtag aggtgtacta ttctagttcc aatctactat ataaatgaaa
    64321 accttagctg tttcgccaag gttcatttct tctatgcaag ttcttctctt ttcttatctg
    64381 ggttccagat gaagcttgcg ataaagctaa agatgatggt taggatagcg aggataatag
    64441 caaggatgag ggcagggatg cggataaacc accagagtgg atttggtttt ttatcattgt
    64501 gccattgttt cgtttcttcg tccagacgtt ggaattctgt ttgaatacga ttggcgactg
    64561 tttcaatgcc ttcatcattc attttcttga tatctgagat atcgattgga tttccaaagt
    64621 tcatatcgac acgttcacgg ctaatcaagc ccttcaaagt catgggaccg gtgtaggtaa
    64681 ccggcatgat acggaccttg gccattttgg caatcagtgc tgcgcccccc ttgacatcgt
    64741 ttgagtggcg gctaccactt ggaaacatga tgagagagcg gtcacttttt ttgagaacgt
    64801 tgataggata tttgatggct gaggcgctgg gattttcacg gtcgatggga aaggcgccac
    64861 acatacgaat ccaccaacca aagatacggt tggtaaagag ttcttttttt gccataaaga
    64921 tgaactgttt tggcttggtc gcaaaggcca tataaacagg atcccaccag gtacggtgag
    64981 gcgcaactaa aatataattt tcatcttgat taggaatttt attagtatta tgatagtgag
    65041 cattgccatt gatggaccat aagagcaata caactaatcc acgcaaataa gtataaaaca
    65101 tgcgatctcc ttcgattgtt ttcttgttat tattatacct tatcaaagga gggctggcaa
    65161 acttttccct tgactagatg catatttggg atgaaattag aattctgtta aaaaaaatga
    65221 tataatagaa tttatggata aaaataagat tatgggatta acccaaagag aagtcaagga
    65281 aagacaggct gagggtttgg tcaatgactt taccgcatca gccagtacca gcacttggca
    65341 aatcgttaaa cgaaatgtct ttaccctttt taacgctttg aactttgcca ttgctttggc
    65401 tcttgccttt gtgcaggctt ggagcaatct ggtcttcttt gctgttatct gctttaacgc
    65461 tttttctggg attgtgaccg agctacgagc caaacacatg gtggacaagc tcaatctcat
    65521 gaccaaggaa aaggtcaaaa ccatccgtga tggtcaggaa gttgctctta atcctgaaga
    65581 attagtgcta ggagatgtca ttcgtttgtc tgcaggagag cagattccta gtgatgcctt
    65641 ggttttggaa ggctttgcgg aagtcaatga agccatgtta acgggagaaa gtgatttggt
    65701 gcaaaaggaa gttgacggct tacttttgtc aggaagtttc ctagccagtg ggtcagtttt
    65761 atctcaagtt caccatgtcg gtgcagacaa ctatgctgcc aaactcatgc ttgaggctaa
    65821 gaccgttaaa cccatcaact cccgtatcat gaaatcgctg gacaagttgg ctggttttac
    65881 tgggaagatt atcattccct ttggtctggc tctcttgctg gaagccttgc ttttaaaagg
    65941 cctgcctctc aagtcatccg ttgtaaactc gtcgacagct cttttgggaa tgttgcctaa
    66001 gggaattgcc cttttgacca ttacttcgct cttgactgca gtgattaagt tgggcttgaa
    66061 aaaggtcttg gtgcaggaga tgtactctgt tgagaccttg gcgcgcgtgg atatgctctg
    66121 tctggacaag acgggcacca tcacccaagg aaagatgcag gtggaggctg ttcttccact
    66181 gacggaaact tacggtgaag aggctattgc cagcatcttg actagctaca tggcccatag
    66241 tgaggataag aatccaactg cccaagccat tcgccagcgt tttgtgggag atgttgctta
    66301 tcctatgatt tccaatcttc ccttctcgag cgaccgcaag tggggggcta tggagttaga
    66361 aggcttgggg acagttttct taggggcacc tgagatgttg ctggattctg aagtcccaga
    66421 agccagggag gccttggaga gaggatcacg tgtcttggtc ttagctctca gtcaggagaa
    66481 attagaccat cacaaaccac agaaaccatc tgatattcag gctctagcct tgctggaaat
    66541 cttggacccc attcgagagg gagcagcaga gacgctggac tatctccgtt ctcaggaggt
    66601 gggactcaag attatctctg gtgacaatcc agttacggtg tccagcattg cccagaaggc
    66661 tggttttgcg gactatcaca gctatgtaga ttgctcaaaa atcaccgatg aggaattgat
    66721 ggccatggcg gaggagacag ctattttcgg acgtgtttcc cctcatcaaa agaaactcat
    66781 catccaaacg ttgaaaaaag cgggtcatac aacggctatg acaggggacg gggttaatga
    66841 tatcttggcc cttcgtgagg cggattgttc tatcgtgatg gcggaggggg atccagcaac
    66901 ccgtcagatt gccaatctgg ttctcttgaa ctcagacttt aatgatgttc ctgagattct
    66961 cttcgagggt cgtcgcgtgg tcaataacat tgcccacatc gccccgattt tcttgataaa
    67021 gaccatctat tccttcctgt tagcagtcat ctgtattgcc agtgctttac taggtcggtc
    67081 agagtggatt ttgattttcc ccttcattcc gatccagatt accatgattg accagtttgt
    67141 ggaaggtttc ccaccattcg ttctgacttt tgagcgaaat atcaaacctg tcgagccaaa
    67201 cttcctcaga agatccatgc ttcgtgccct accaagtgcc ctcatggtcg tgtttggcgt
    67261 tctttttgtg aaaatctttg gaacgagtca aggttggtct gagttagaaa tctcaactct
    67321 cctctattat ctcttggggt caattggttt cttatccgta tttagagcct gcatgccatt
    67381 cactctatgg cgagtcctat tgattgtctg gtcagtagga ggcttcctag ccacagctct
    67441 cttctcaaga attcaaaaac tgcttgaaat tgcaacctta acaggacaaa cattacctgt
    67501 ttatggtgtc atgatgttgg tctttaccgt gattttcatc ctgaccagtc gttaccaagc
    67561 gaaaaaataa agaaagactg caatctgtgg attgcagttt ttttaggtac aagattgcca
    67621 gccgaaatgt ggtataataa gaggtaatag agttttggaa agtgagagaa gatgatttca
    67681 aagagattag aattggtagc ttcctttgtg tcacaggggg ctattttact agatgtggga
    67741 agtgaccatg cttatctgcc tatcgagttg gttgagagag gccaaatcaa aagcgctatt
    67801 gcaggtgagg tggtggaagg tccctatcag tctgcggtta aaaatgttga ggctcacggc
    67861 ctaaaggaga aaatccaagt ccgtttagcc aatggcttgg cagcttttga agagactgac
    67921 caagtgtctg tcattaccat tgctggcatg ggtggttgtt tgattgctag gattttagaa
    67981 gaaggtttgg ggaagttagc taatgtagag cgtttgatcc tccagcccaa taatcgtgaa
    68041 gacgacttgc gtatctggct acagaatcat ggattccaga ttgtagcaga aagcatctta
    68101 gaagaagctg gcaagtttta cgagattttg gtggtggaag caggacaaat gaagctatca
    68161 gccagtgatg ttcgctttgg tcccttcttg tccaaagaag tcagtccagt atttgtccaa
    68221 aaatggcaaa aagaagctga gaagctagag tttgccctcg gacaaatccc agaaaaaaat
    68281 ctggaggaac gtcaagttct agtagataag attcaagcta tcaaggaggt gctccatgtt
    68341 agcaagtgaa gtgatccaag cttatgaagc cttttgccct caggaatttt ctatggaggg
    68401 agacagccgt ggtctgcaaa ttggcacttt agacaagggt atccaaaggg tcatggttgc
    68461 cctcgatatt cgtgaagaga cggtggcaga ggccattgaa aagggtgtgg acttgattat
    68521 cgtcaagcac gcgcctatct ttcgtcctat caaggacttg cttgccagcc gtccgcaaaa
    68581 tcagatttac atcgacctga ttaagcatga catcgcagtt tatgtcagcc ataccaatat
    68641 tgatatcgtt gaaaatgggc tcaatgactg gttttgtcag atgctaggaa tcgaggagac
    68701 gacttatctg caggaaacag gtccagaacg tggaattgga cgtattggga atattcagcc
    68761 tcagacattt tgggaattgg cccaacaggt caagcaagtc tttgacctag atagccttcg
    68821 aatggtgcat tatcaagaga atgatttgca gaagcctatt tcaagagtag caatttgtgg
    68881 tggaagcggg cagtctttct ataaggatgc tttggcaaag ggggcagatg tctatatcac
    68941 tggtgatatc tactaccata ctgctcagga tatgctgtct gatggcttgt tagcattgga
    69001 tccaggtcac tatatcgaag tgctttttgt ggaaaaaatc gcagcactcc ttactcaatg
    69061 gaaagagaaa aaaggctggg aactagagat tttacctagt caagcatcga ccaatccttt
    69121 ccgtcatatc tagttagaaa gtgaagacaa tgaaaaaagt tgccattatt ggagcaggaa
    69181 ttgtgggagc aacagctgcc tactacctct cgagagaaag tgacctagag gtgaccgttt
    69241 ttgaccatga gcaaggtcaa gccaccaagg ccgcagcagg aattatcagt ccttggtttt
    69301 ccaaacgccg taataaagcc tggtacaaga tggcgcgctt gggggctgac ttttatgtgg
    69361 atttattagc tgatttagag aaatcaggac aaaaaatcga cttttaccag cgttcgggag
    69421 tctttctctt gaaaaaggat gaatccaagt tggaagaact ttatcaactg gccctccagc
    69481 gcagagaaga atctcccttg atagggcaat tagccattct ggaccaagct tcagctaatg
    69541 aattattccc tggtttgcag ggatttgacc gcctgctcta tgcttctggt ggagcgagag
    69601 tagatggcca actcttggta actcgtctgc tagaagccag tcatgtcaag ctggtcaaag
    69661 aaaaagtgac tctgacactc ttatcatcag gctaccagat tggcgaagag gtgtttgatc
    69721 aggttatttt ggcaacggga gcttggttgg gggacatgtt agagccttta ggttatgaag
    69781 tggatgtccg tcctcaaaaa ggacaactac gagattatca gcttgcccaa gacatggaag
    69841 attaccctgt tgtcatgcca gaaggggagt gggatttgat tccctttgca ggtgggaaat
    69901 tatccttggg cgctacccat gaaaatgaca tgggatttga tttgacggta gatgaaccct
    69961 tgctccaaca aatggaggag gcggccttgc ctcattaccc agttttagct agagcgactt
    70021 caagggctga gcgtgtggga atccgtgcct ataccagtga tttctctccc ttctttgggc
    70081 aggtgccaga attggcaggt gtctatgtag ccagtggact aggttcatca ggcctcacaa
    70141 ctggtcctat cattggttac catctagccc aactgatcca agacaaggag ttgaccttgg
    70201 accctctaaa ttatccaatt gaaaactatg tcaaacgagt aaaaagcgaa taatactcaa
    70261 tgaaaatcaa agaacaaact aggaaactag ccgcgggtta ctcaaagcac tgctttgagg
    70321 ttgtgtatag aactgacgaa gtcagtaacc atacccatgg caaggcgacg ttgacgtggt
    70381 ttgaattgga ttttcgaaga gtataagcat tttactgaaa ttttatcagg tagtttagga
    70441 tggcaaatga cattccctgt caaaaatggt aaaataagaa aaaataatct gagaatcgag
    70501 gaaaaaagat gcaagaaaag attttggtaa ctggtggtgc cggttttatt ggaacccaca
    70561 ctgttattga attgatccaa gcaggccatc aagttgttgt ggtggataat cttgtcaata
    70621 gcaatcgtaa gagtttagaa gttgttgaaa gaatcacagg agttgagatt cctttctatg
    70681 aggcagatat tcgtgacaca gttaccctca gagatatttt caagcaggaa gaaccgactg
    70741 gtgtcattca ctttgctggt ttgaaggctg ttggcgaatc aacacgtatc cctcttgcct
    70801 actatgacaa caatatcgct ggaactgtca gccttttgaa agccatggaa gaaaacaact
    70861 gtaaaaacat catcttcagt tcttctgcga cagtttacgg ggatccgcac acagtgccca
    70921 tcttggaaga tttcccactt tcagtgacca acccatacgg tcgtaccaag ctcatgctag
    70981 aggaaatttt gaccgatatt tacaaagcag actcagaatg gaatgttgtc ttgcttcgtt
    71041 actttaaccc aatcggagcc catgagagtg gtgatttggg agaaaatcca aacggtattc
    71101 caaacaatct cttgccatat gtgactcaag tagccgttgg aaaattagag caagtgcaag
    71161 tgtttggaga cgattacgat acggaagatg gaacaggtgt tcgtgactat atccacgttg
    71221 tcgatttggc taagggtcac gttgcagctt tgaaaaaaat ccaaaaaggt tcaggactaa
    71281 acgtttataa ccttggaact ggtaaaggtt actcagttct tgaaattatc caaaacatgg
    71341 aaaaagcggt gggacgtcct attccttacc gcatcgtaga acgtcgccca ggtgatatcg
    71401 ctgcctgcta ctcagaccca gcaaaagcta aagcagaact cggttgggaa gcagaactcg
    71461 acatcaccca aatgtgtgaa gacgcatggc gttggcaaag caagcatcca aatggatttg
    71521 aagactaaga tgatgatttc aatcatcgtc ccttgtttaa acgaagagga agtacttcct
    71581 cttttttatc aggctctgga agctttactt ccagatttgg aaacagaaat cgagtatgtc
    71641 tttgtcgatg atggatcaag tgatgggacc ttggaactct taaaggccta tcgggagcaa
    71701 aatccggcag tccattatat ttctttctct cgaaattttg gcaaagaagc agccctttat
    71761 gcaggcttgc aatatgcgac aggagatttg gtggtggtga tggatgcaga cctccaagat
    71821 cctcctagta tgttgtttga gatgaaaaat gtactagaca aaaatgtaga cttggactgc
    71881 gttgggacac ggagaactag tcgggaggga gaacccttct ttcgcagttt ctgtgctgtt
    71941 ctcttttatc gcctcatgca aaaaatcagc ccagtagctc tgccgtcggg tgtccgtgac
    72001 tttcgtatga tgagaaggtc tgtggtcgat gccattttaa gcttgactga gtccaatcgt
    72061 ttttctaagg gactctttgc ctgggtcggc tttaaaaccc actatctgga ctatccaaat
    72121 gtcgaaaggc aggctggcaa gaccagttgg agttttaggc aactcttttt ttactccatt
    72181 gaagggattg ttaatttttc agatttccct ttgactatag cctttgtagc tggtctccta
    72241 tcttgttttc tttctctgct gatgaccttt tttgttgtgg ttcggaccct cattttgggc
    72301 aatccgacat ctggttggac ctctctgatg gctgttattc tctttcttgg aggcattcaa
    72361 ctcttgacca ttgggattct cggtaagtat atcagtaaga tttatttaga gactaaaaaa
    72421 agaccacttt atcttatcaa agaaaaaagt gaccttcctg attttacaga aaaaaataaa
    72481 gtgaaaagac tataatttta catggaaatg tgctaaacta gagggagtgg attatcgcca
    72541 ttgatttagg cttcctggtt tccaatcagg gcgcgagttg ggcaattttt aacagcctct
    72601 aagacatcct gactaggaga aatttctttt tccagttggt cagggtcatc gtaaaaacgc
    72661 acgattccat tatcgtggta atcaaataaa tcagaataag tttggcaaag tccacaggcg
    72721 atacatcgtt caggtataag tgtgattttc atgttcatat ttatattgta ataagaaaag
    72781 taaaaaaaaa caaggagtaa ggtatggcaa aagaaccgtg gcaagaagat atttatgatc
    72841 aagaagaatc aagagcaggg cgtcggcatc gaaaccacgg aggggctgat aggatggcta
    72901 atcgtatttt gacgatccta gctagtattt tctttgtaat tgtggtggtg atggtcatcg
    72961 ttctcatcta tctatcatcg ggggggagta atcgcacagc agccttaaaa gactttcatg
    73021 attctgatgc aagtgtagta caaatctcat cttcaagcag ttctcagcct gagcagagtt
    73081 cagagccaga atctacttct agtagttcag aagaagctgc taatcctgaa ggaacgatta
    73141 aagttctcgc aggagaaggg gaagcagcta ttgccgctcg tgcaggaatc tccattgctc
    73201 agttagaggc cttgaatcct gggcacatgg ctacaggatc ttggtttgct aatccaggtg
    73261 atgttataaa aataaaatag gagccgatat gaaaacaatt caaattgcta ttgatggtcc
    73321 tgcttccagc ggtaagagta cggtcgcaaa gattattgct aaggattttg gattcaccta
    73381 ccttgataca ggagctatgt atcgtgcagc gacctatatg gctcttaaga accaattagg
    73441 agttgaagaa gtcgaagccc ttctagcctt gttggaccag catccaatca gctttggacg
    73501 ttcagaaact ggagaccagc ttgtttttgt aggagatgtg gatattaccc atcctatccg
    73561 tgaaaatgaa gtgaccaatc atgtttctgc tattgcagca attcctgaag tgcgtgagaa
    73621 actggtttct ctccaacaag aaattgccca gcaaggcggg attgtcatgg atggtcgcga
    73681 tattggaact gttgtattgc cacaagcaga attgaaaatt ttcctagtag cttctgttga
    73741 tgagagagca gagcgtcgtt acaaggaaaa tattgccaag ggaattgaaa cagaccttga
    73801 aaccctaaaa aaggaaattg ctgcgcgtga ctacaaggat agtcatcgtg agacttctcc
    73861 tctcaaacaa gcagaggatg ctgtctacct tgatacaact ggtttgaaca ttcaagaagt
    73921 agttgaaaaa atcaaagcag aagctgaaaa aagaatgtaa atgtagaaaa gccgatagat
    73981 tgatatcggt ttttttctaa atttgtcaaa atgctgattt tagggtataa tagttgctgt
    74041 tcgagaaatt ttgattttca aagaatagtg aatgatgata aggagaaacc atgaacaatt
    74101 taccaaattg tccaaaatgt aactcagagt atgtctacga agacggtgcc ctactggttt
    74161 gcccagagtg tgctcatgag tggaatcctg ctgaagttgc agaagtagaa gaaggtcttg
    74221 tcgctatcga tgccaacgga aataaattgg ctgatggtga tatagtaact ctcatcaagg
    74281 acttgaaagt aaaaggtgcg ccaaaagatt tgaaacaagg gacgcgcgtg aaaaatatcc
    74341 gcatcgtaga aggcgaccac aatatcgact gtaagattga tggttttggt gccatgaaac
    74401 ttaaatcaga gtttgtgagg aagatttaag catgtcaaaa cactataaac ttgtatttta
    74461 tagccgtatc ttcttgtttc tagcggcttt tacgggagtt tatcttgaaa ttactaggca
    74521 tggtggtttt gggatgcttc tctattacac ggttctgtcc aacctcttgg taactatttt
    74581 taccctttat cttctaaagg ttatgagccg tgtaggtgaa aattggcaaa ggccaagtct
    74641 cttgcgctta aaaggtgggg tcaccatgag tatcatgatt acctgtgtga tttaccattt
    74701 cctcttggcg cccattgcga ctaatttcta taccctagaa aatttccttt gccactatat
    74761 cgttcccatc tggtttttag cggataccct cttttttgac aaacagggtc aatacaagat
    74821 ttgggatcca gcagtgtgga ccattttacc ctttctgtat atgatgtttg ctctttttaa
    74881 tggcttggtt ctaaaactca atattccaaa tgccaaggat aatcctttcc cttacttctt
    74941 tttgaatgtg aacaagggtt ggaatgttgt gtttaagtgg tgtctgatta tctttgttgc
    75001 ctatatggta gcaggattta ttttctactt tatcaagcaa atcaagagaa agtcatccta
    75061 atactcttcg aaaatctctt caaaccacgt cagtttccat ctgcaacctc aaaacactgt
    75121 tttgaacaac ctgcggctag cttcctagtt tgctctttga ttttcattga gtataagata
    75181 caagggattc attttcgaat ttaagaagga gtctacaaac caatgaggct tcttcttttc
    75241 ttgcttattt gataactatc aaaaaagtag aagattaaga gaaatataga aagagatttc
    75301 atgaaacaat ttttagaacg ggccagcatt ttggctctct ccctcgtttt gattacctcc
    75361 ttttccattt cgagtgccct accagccatg tttgactatt atcagggtta ttctaaggaa
    75421 caaattgagc tcttggtgag cttgccttcc tttggaatca tgatgatgtt actgctaaat
    75481 ggtttcttag aaaaaatatt tcctgagcgc ttacagatta gtttgggctt gctgatttta
    75541 tcattgagcg gtacagctcc cttctggtac caagcctatc cctttgtctt tggaacacgg
    75601 cttctctttg gtttgggtct tgggatgatc aatgccaagg ccatttctat tatcagtgaa
    75661 cgctatcaag gaaaaaggcg aattcagatg ttagggctac gcgcttctgc agaggtcgtt
    75721 ggagcttctc tcattacctt ggccgtcggt cagttgttgg cctttggttg gacagctatc
    75781 tttctagcct atagtgctgg atttttggtg ctgccccttt atctgctctt tgtcccttat
    75841 ggaaaatcaa agaaagaagt caagaaaaga gcgaagaaag caagtcgttt aactcgagaa
    75901 atgaaaggct tgatttttac cttagctatc gaagcggcag ttgtagtttg taccaataca
    75961 gctattacca tccgtattcc aagtttgatg gtggaaagag gattggggga tgcccagtta
    76021 tctagttttg ttcttagtat catgcagttg atcgggattg tggctggggt gagtttttct
    76081 ttcttgattt ctatctttaa agagaaactg ctcctctggt ctggtattac ctttggcttg
    76141 gggcaaatcg tgattgcctt gtcttcatcc ttgtgggtgg tagtagcagg aagtgttctg
    76201 gctggatttg cctatagtgt agtcttgacg acggtctttc aacttgtctc tgaacgaatt
    76261 ccagctaaac tcctcaatca agcaacttca tttgctgtat taggctgtag tttcggagcc
    76321 tttacgaccc catttgttct aggtgcaatt ggcttactaa ctcacaatgg gatgttggtc
    76381 tttagtatct taggaggttg gttgattgta atctctatct ttgtcatgta cctacttcag
    76441 aagagagctt aggattgatt cctaagtttt tcttttgata ctcaatgaaa atcaaagagc
    76501 gtagttgatt gagtttggaa tagtacgctg tagctgctaa aatatttcta gaaattaatt
    76561 tgactttcct aatagagttg ttcatatctt atttcaatct actatagaaa gctagccgca
    76621 ggttgctcaa aatagtgttt tgaggttgca gatggaaact gacgtagttt gaagagattt
    76681 tcgaagagta tgagattttt atacccatac aatatttgtt ttaggtcctt gtttacagac
    76741 taatttcttg ataaaataga ggttatgacg agatataaag caactatttc ctatgatggt
    76801 tatgcctttg ctggctttca gcgccagcct catgcgcgta gcgttcagga agaaattgaa
    76861 aaaaccttga ccagattaaa taaagggcaa accattactg ttcacggtgc tggtaggaca
    76921 gatagtgggg ttcatgccct gggacaggtc attcattttg acctgcctta tcagatggat
    76981 gaggagaaac tccgttttgc cttggatacc cagtctcctg aagatattga tgtgatttcg
    77041 attgagcttg tggcagatga ttttcattgc cgttatgcca aacatagcaa gacctatgag
    77101 tttattgtgg atagaggacg tcccaaaaat cctatgcgcc gtcactatgc cacccacttt
    77161 ccctacccac tcgatgtgga acgaatgcag attgcaatca aaaagctaga gggaacccat
    77221 gattttaccg gttttacagc ctctgggact agtgtagagg ataaggttcg caccatcaca
    77281 gaagctagtt taatagtcga tgagacagga caatttttga cctttacctt ttcaggaaat
    77341 ggtttcttgt ataaacagat tcgcaatatg gtggggactc ttctcaaaat cggtaacaac
    77401 cgcatgccag tagagcagat tgctctcatc ttggagaaga aggacaggca acttgcaggt
    77461 cccactgcag caccaaatgg tttgtattta aaggagattc gttatgaaga ataatcgtat
    77521 tttagcactt tctggaaatg atatttttag tggtggtgga ctgtcagctg atttggctac
    77581 ctataccttg aacggcttgc atgggtttgt agcagtgact tgtttgacag ccttgacaga
    77641 aaaaggattt gaagtctttc caactgatga taccattttt caacatgaat tagatagctt
    77701 gcgtgatgtg gaatttgggg gaattaagat tggtcttctc cctactgtca gtgtggctga
    77761 gaaggccttg gactttatca aacaacgccc aggagtacct gtggtgttgg atcctgtctt
    77821 ggtctgcaag gaaacgcatg atgtagctgt cagtgagctc tgccaagagt tgattcgctt
    77881 cttcccttat gtcagtgtga ttacgcctaa tctcccagaa gcagaattat tatccggtca
    77941 ggaaattaaa accttggaag acatgaaaac tgcagcgcag aaattgcatg atttaggagc
    78001 gccagcagtc attatcaagg gaggcaatcg tcttagtcag gacaaggctg tggatgtctt
    78061 ttatgatgga cagaccttta ctatcctaga aaatccagtt atccaaggcc aaaatgctgg
    78121 tgcaggttgt acctttgcct ctagcattgc cagtcacctg gttaaaggtg ataaactttt
    78181 gccagcagta gaaagctcta aggctttcgt ttatcgtgct attgcacaag cagatcagta
    78241 tggagtaaga caatatgaag caaacaaaaa caactaaaat cgcccttgta tccctattaa
    78301 ccgccctttc tgtggttcta ggttatttct taaaattccc aacacctaca ggaattctaa
    78361 ctcttttaga tgctggtgtc ttctttgcgg ccttttactt tggtagtcgt gaaggagcgg
    78421 tagtcggagg actagcaagt ttcttgattg acctcttatc aggctaccct cagtggatgt
    78481 tctttagctt ggtcaaccat ggcttgcagg gatttttcgc aggatttaaa ggaaaaagtc
    78541 agtggttagg ccttatttta gcaactattg ccatggtagg aggctacgcc ttgggttcta
    78601 ctttgatgaa tggctgggca gcagccctcc cagaaattct accgaatttt atgcaaaata
    78661 tggtagggat gattgtagga tttattctta gtcaaagtat caagaagatt aagtaatact
    78721 cttcgaaaat ctcttcaaac cacgtcagct tccatctgca acctcaaaac actgttttga
    78781 gcagcctgag gctagctttc tagtttgcac tttgattttc attgagtata aagaggttga
    78841 gtaaaaagtc tttcaaataa gaaaaacgca ttatatcaag tcttgactga actgcacccc
    78901 aaaagttaga cagaaaaaat ctaacttttg gggtgttttt attatgaaa
//
LOCUS       DAVLYV010000002        66550 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLYV010000002 DAVLYV010000000
VERSION     DAVLYV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 66550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.002"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..1452)
                     /gene="pfbA"
                     /locus_tag="VYO70_000077"
     CDS             complement(<1..1452)
                     /gene="pfbA"
                     /locus_tag="VYO70_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1712385.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLG"
     gene            complement(1765..1995)
                     /locus_tag="VYO70_000078"
     CDS             complement(1765..1995)
                     /locus_tag="VYO70_000078"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712386.1"
                     /translation="MSDIQVNIPGECLYDKVFVLSFIIYNISTNLNIVNGIFYIQAKK
                     DSITFEWKAKEQTRKLAIDSSKPCFEVVDIVK"
     gene            complement(2615..3841)
                     /locus_tag="VYO70_000079"
     CDS             complement(2615..3841)
                     /locus_tag="VYO70_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1712387.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3884..4576)
                     /locus_tag="VYO70_000080"
     CDS             complement(3884..4576)
                     /locus_tag="VYO70_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1712388.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNCRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4749..6500)
                     /locus_tag="VYO70_000081"
     CDS             complement(4749..6500)
                     /locus_tag="VYO70_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712389.1"
                     /translation="MQNKQEQWAVLKRLMSYLKSYGLLIFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGFSIG
                     ITVGTMYAFIQYINRLFDPLIEVTQNFSTLQTAMVSAGRVFALIDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKVLILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(6490..8235)
                     /locus_tag="VYO70_000082"
     CDS             complement(6490..8235)
                     /locus_tag="VYO70_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1712390.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALILVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8441..8818)
                     /locus_tag="VYO70_000083"
     CDS             complement(8441..8818)
                     /locus_tag="VYO70_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1712391.1"
                     /translation="MKRLIDGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(8890..9346)
                     /locus_tag="VYO70_000084"
                     /pseudo
     CDS             complement(8890..9346)
                     /locus_tag="VYO70_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            9480..10307
                     /gene="xth"
                     /locus_tag="VYO70_000085"
     CDS             9480..10307
                     /gene="xth"
                     /locus_tag="VYO70_000085"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1712392.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            10319..10417
                     /locus_tag="VYO70_000086"
     CDS             10319..10417
                     /locus_tag="VYO70_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1712393.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEY"
     gene            10462..10701
                     /locus_tag="VYO70_000087"
     CDS             10462..10701
                     /locus_tag="VYO70_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1712394.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRLLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      11084..11179
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11579..12160
                     /locus_tag="VYO70_000088"
     CDS             11579..12160
                     /locus_tag="VYO70_000088"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1712395.1"
                     /translation="MKLLEERILKDGHILDDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVLFKEADL"
     gene            12160..13422
                     /locus_tag="VYO70_000089"
     CDS             12160..13422
                     /locus_tag="VYO70_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1712396.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIFVASLLAIVLNAVLNHKKK"
     gene            complement(13473..13934)
                     /locus_tag="VYO70_000090"
     CDS             complement(13473..13934)
                     /locus_tag="VYO70_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1712397.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(13931..14695)
                     /locus_tag="VYO70_000091"
     CDS             complement(13931..14695)
                     /locus_tag="VYO70_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HEV1712398.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(14876..15280)
                     /locus_tag="VYO70_000092"
     CDS             complement(14876..15280)
                     /locus_tag="VYO70_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1712399.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(15350..16831)
                     /locus_tag="VYO70_000093"
     CDS             complement(15350..16831)
                     /locus_tag="VYO70_000093"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1712400.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKAQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGISLD"
     gene            complement(16850..18028)
                     /locus_tag="VYO70_000094"
     CDS             complement(16850..18028)
                     /locus_tag="VYO70_000094"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1712401.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            18192..19199
                     /gene="galR"
                     /locus_tag="VYO70_000095"
     CDS             18192..19199
                     /gene="galR"
                     /locus_tag="VYO70_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1712402.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(19245..20282)
                     /locus_tag="VYO70_000096"
     CDS             complement(19245..20282)
                     /locus_tag="VYO70_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1712403.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(20283..20636)
                     /locus_tag="VYO70_000097"
     CDS             complement(20283..20636)
                     /locus_tag="VYO70_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1712404.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(20880..21770)
                     /locus_tag="VYO70_000098"
     CDS             complement(20880..21770)
                     /locus_tag="VYO70_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1712405.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            21903..22439
                     /locus_tag="VYO70_000099"
     CDS             21903..22439
                     /locus_tag="VYO70_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1712406.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            complement(22605..23411)
                     /locus_tag="VYO70_000100"
     CDS             complement(22605..23411)
                     /locus_tag="VYO70_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1712407.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(23756..25276)
                     /locus_tag="VYO70_000101"
     CDS             complement(23756..25276)
                     /locus_tag="VYO70_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1712408.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(25269..25997)
                     /locus_tag="VYO70_000102"
     CDS             complement(25269..25997)
                     /locus_tag="VYO70_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712409.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKVPATDFVADLFGGSVHD"
     gene            complement(26012..26776)
                     /locus_tag="VYO70_000103"
     CDS             complement(26012..26776)
                     /locus_tag="VYO70_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1712410.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTETFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(26780..27223)
                     /locus_tag="VYO70_000104"
     CDS             complement(26780..27223)
                     /locus_tag="VYO70_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1712411.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKENEV"
     gene            complement(27905..28201)
                     /locus_tag="VYO70_000105"
     CDS             complement(27905..28201)
                     /locus_tag="VYO70_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1712412.1"
                     /translation="MVEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSSLVNRELLLNPKHPAPELLNLARFVKREEAKYRGTATSALMYEELFKML"
     gene            complement(28223..29287)
                     /gene="pepA"
                     /locus_tag="VYO70_000106"
     CDS             complement(28223..29287)
                     /gene="pepA"
                     /locus_tag="VYO70_000106"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1712413.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            29688..30389
                     /gene="galE"
                     /locus_tag="VYO70_000107"
     CDS             29688..30389
                     /gene="galE"
                     /locus_tag="VYO70_000107"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1712414.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            30812..31771
                     /locus_tag="VYO70_000108"
     CDS             30812..31771
                     /locus_tag="VYO70_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712415.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSILFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            31761..32717
                     /locus_tag="VYO70_000109"
     CDS             31761..32717
                     /locus_tag="VYO70_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1712416.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            32714..33466
                     /locus_tag="VYO70_000110"
     CDS             32714..33466
                     /locus_tag="VYO70_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1712417.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            33562..34527
                     /locus_tag="VYO70_000111"
     CDS             33562..34527
                     /locus_tag="VYO70_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1712418.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQKVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTTFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34752..34994)
                     /gene="yidD"
                     /locus_tag="VYO70_000112"
     CDS             complement(34752..34994)
                     /gene="yidD"
                     /locus_tag="VYO70_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1712419.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(34994..35716)
                     /locus_tag="VYO70_000113"
     CDS             complement(34994..35716)
                     /locus_tag="VYO70_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1712420.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(35703..36272)
                     /locus_tag="VYO70_000114"
     CDS             complement(35703..36272)
                     /locus_tag="VYO70_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1712421.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(36291..37019)
                     /locus_tag="VYO70_000115"
     CDS             complement(36291..37019)
                     /locus_tag="VYO70_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1712422.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(37019..37759)
                     /gene="xerD"
                     /locus_tag="VYO70_000116"
     CDS             complement(37019..37759)
                     /gene="xerD"
                     /locus_tag="VYO70_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1712423.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(37750..38211)
                     /locus_tag="VYO70_000117"
     CDS             complement(37750..38211)
                     /locus_tag="VYO70_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1712424.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(38208..38729)
                     /locus_tag="VYO70_000118"
     CDS             complement(38208..38729)
                     /locus_tag="VYO70_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1712425.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(38705..39676)
                     /locus_tag="VYO70_000119"
     CDS             complement(38705..39676)
                     /locus_tag="VYO70_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1712426.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(39673..40467)
                     /gene="racE"
                     /locus_tag="VYO70_000120"
     CDS             complement(39673..40467)
                     /gene="racE"
                     /locus_tag="VYO70_000120"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1712427.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            complement(40674..40922)
                     /locus_tag="VYO70_000121"
     CDS             complement(40674..40922)
                     /locus_tag="VYO70_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1712428.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(41064..42689)
                     /gene="treC"
                     /locus_tag="VYO70_000122"
     CDS             complement(41064..42689)
                     /gene="treC"
                     /locus_tag="VYO70_000122"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1712429.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(42867..44834)
                     /gene="treP"
                     /locus_tag="VYO70_000123"
     CDS             complement(42867..44834)
                     /gene="treP"
                     /locus_tag="VYO70_000123"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1712430.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QCVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            45019..45729
                     /gene="treR"
                     /locus_tag="VYO70_000124"
     CDS             45019..45729
                     /gene="treR"
                     /locus_tag="VYO70_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1712431.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(45762..46565)
                     /locus_tag="VYO70_000125"
     CDS             complement(45762..46565)
                     /locus_tag="VYO70_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1712432.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGRN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(46578..47455)
                     /locus_tag="VYO70_000126"
                     /pseudo
     CDS             complement(46578..47455)
                     /locus_tag="VYO70_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47467..48327)
                     /locus_tag="VYO70_000127"
                     /pseudo
     CDS             complement(47467..48327)
                     /locus_tag="VYO70_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(48895..49821)
                     /locus_tag="VYO70_000128"
     CDS             complement(48895..49821)
                     /locus_tag="VYO70_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712433.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQTELARYQKGLN"
     gene            complement(49832..50899)
                     /locus_tag="VYO70_000129"
     CDS             complement(49832..50899)
                     /locus_tag="VYO70_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712434.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            complement(50908..51834)
                     /locus_tag="VYO70_000130"
     CDS             complement(50908..51834)
                     /locus_tag="VYO70_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712435.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            complement(51834..53330)
                     /locus_tag="VYO70_000131"
     CDS             complement(51834..53330)
                     /locus_tag="VYO70_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712436.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGTLTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPVTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            complement(53397..55376)
                     /locus_tag="VYO70_000132"
     CDS             complement(53397..55376)
                     /locus_tag="VYO70_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1712437.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNVDKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAARETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            complement(56062..57255)
                     /locus_tag="VYO70_000133"
     CDS             complement(56062..57255)
                     /locus_tag="VYO70_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1712438.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            complement(57393..58835)
                     /gene="gtfA"
                     /locus_tag="VYO70_000134"
     CDS             complement(57393..58835)
                     /gene="gtfA"
                     /locus_tag="VYO70_000134"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1712439.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFRDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVVEIDLQNQTYRVIENGVEVSF"
     gene            complement(59112..59192)
                     /locus_tag="VYO70_000135"
                     /pseudo
     CDS             complement(59112..59192)
                     /locus_tag="VYO70_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(59193..60026)
                     /locus_tag="VYO70_000136"
     CDS             complement(59193..60026)
                     /locus_tag="VYO70_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1712440.1"
                     /translation="MKQDERKALIGKYILLILGTVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            complement(60040..60906)
                     /locus_tag="VYO70_000137"
     CDS             complement(60040..60906)
                     /locus_tag="VYO70_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1712441.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            complement(60920..62179)
                     /locus_tag="VYO70_000138"
     CDS             complement(60920..62179)
                     /locus_tag="VYO70_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1712442.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            complement(62271..64433)
                     /locus_tag="VYO70_000139"
     CDS             complement(62271..64433)
                     /locus_tag="VYO70_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1712443.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENQNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHTQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRRHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMMRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGLVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            64541..65401
                     /locus_tag="VYO70_000140"
     CDS             64541..65401
                     /locus_tag="VYO70_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1712444.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            65398..66333
                     /locus_tag="VYO70_000141"
     CDS             65398..66333
                     /locus_tag="VYO70_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1712445.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(66459..66546)
                     /locus_tag="VYO70_000142"
     tRNA            complement(66459..66546)
                     /locus_tag="VYO70_000142"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(66510..66512),aa:Ser,seq:gct)
BASE COUNT        19903 a        14934 c        12316 g        19397 t
ORIGIN      
        1 tcctaaatat tctgcactat cttttgcaac tcgtatcgct tttgttttag gatcggcaat
       61 attaaatata ttttcggcga taaccacctg tttatttaaa tctaataggt ctttagtgtt
      121 tttatagcta taagcattta ctaaagctgc tccgctttct cgataatgta cactctctcc
      181 tttaactttc ttatcaaagc gatttccttt gattaataca tcagtgaatc ctgtaaaacg
      241 tacacctgca tacatcatgt tatcaaaatg attatttaaa attttaatat tagaggggtt
      301 ctgtgtcgac aatgtttgat agtgtgtgcc aattgctgtt actaattccc cagatttatc
      361 acttttgcca aaataggaat tttgaatagt cacattttca gattttttcc catcatcatt
      421 caaggcataa ggaaaacctt ttctagttaa tggttcaatc tgaatgctct ccttacttat
      481 gatttgccca tccttcatcg ttttgggtaa ggcttgccca agaaaacgag aattatcaac
      541 taatacattt ttcgaacctg caatttgaat agcatgccct tgataactat ccttgaatgt
      601 tacatttttt atagttacgt tatttgaatt cccaatagca aatgcacctg aagaatttat
      661 aagtggtaga ttttttgctt tagttccttc ttcattcaaa gcaccgttca tatcaatcgt
      721 accaccagaa taactaatat cttctgttgg gccccattct acttgcgcac catcatccgt
      781 aaataaacct gtcataaaaa caatggaagg gtgattctta atatttatac catttagaat
      841 tgtagctttc tcattcaatt ctaagtgtgt atgacttttt aaaaaaacaa tttcttttac
      901 taaataagtt ccttcaggaa aatatacatt tcctccacct agcccttgag ctgcagcatc
      961 tattgcatct tgaattgctt gtcgatcatc atgaatccca tcacctaccg caccataatc
     1021 ttttacactt aaagcagtat cttcaaattc ttttttgata attctgtcac ttataacatc
     1081 ttccttataa ctcttatttt cattagtaga tgttactgta gattcaactg ttctgttgtc
     1141 taattgtgaa ttggaacgaa tagtaccatt agagctgata ctattaacac ttaacgaatt
     1201 atctaaacca ttagtggttg aattagaggc aatcatttca gcagaacgat tctgattaac
     1261 agttggtgaa ttatctaaat cattagtaat tgcattagaa gactcttttg tagccatcgg
     1321 tgaagaacta gtaacaactt catccgataa aataccttgg cttcccaaaa ttgaaattgc
     1381 caatagtacc gagcaagtcc ccacctttaa tttacgaata ctgaatacct cattaggaac
     1441 aaaatatttc atcagaacct ccaatttttt tactattttt aacaattgat tattactttt
     1501 cttaaaatag atagaatgac accgctgtca ttatctttat catatatttt ccaaattgaa
     1561 ttgtcaagga ataacacaag aaaatattaa aatttatact cttcggaaat ctcttcaaac
     1621 taggtcagtg ccgccttgcc ctaagtctag gttactgact tggtcagttc tatagtagac
     1681 tgaaactaaa atagtacacc tctacttcta aaacattgtt agaaatcgat ttgactgtcc
     1741 cgatcaattt gtcctattct tatttcattt tactatatct acaacctcaa agcagggttt
     1801 tgagctatct atggccaact tcctagtttg ctctttagcc ttccattcaa aagtgatgct
     1861 atctttctta gcttgaatat aaaatatccc atttactata tttaaatttg ttgatatgtt
     1921 gtaaattata aatgaaagaa caaatacctt gtcatacaag cattcgccag ggatatttac
     1981 ctgaatatcg ctcatatggc ggattgaaac taaaacacta caatatattt cttaaataga
     2041 ccttctataa aagctagatt taacctcgat aaaaatagat gcagtgcttc taaaatactg
     2101 gaatagggca tataggtaat cgtagacaag tgttttatag attgtctcgc aggagatcta
     2161 atgatgtata gcaagcatca ctaaacatat agaatatttg tattattata atatcaacat
     2221 gctataaatt ctatcatgca aaagaatgac tagaacatga aagacaggca caaaattttg
     2281 aacaatattc tgaaaacaaa aaaagacgcg agtttccaca atctcactca tggaaactca
     2341 cgtctttcaa aaaatagggt gaattgaaaa ctagaacaag acactagatt ttctaaatga
     2401 tgtctagtaa ggattctaaa tgctacacta aattgctcac atcttatttc aaaccacggg
     2461 actcccacaa gaaaggaaaa tcctataaaa attctctctc tgaggagaaa gaggcctcaa
     2521 ctacaggtgt aaactattat caattgaaaa caacagtttt tacactcaat tagacttttt
     2581 agggacagga tcactttcgc ttgactgtag tttgtcattt ttttgataaa gttagcactt
     2641 tttcagaaac tgttttgaaa gtctcaatga tatagtctac ttcttcatcg cttaatttag
     2701 tatgaagagg gagggtaatt tcattctcaa agaaggcata ggccttagga tagttcgtca
     2761 tatcaaatcc aagattctta taggctgtca agagaggaag cggtttgtag tgaacattac
     2821 ttgcaattcc tgctttagcc aattcttgga tgatgaggtt gcgttcttct aggcttgctc
     2881 cttctacacg ggtgatgtag aggtggcgtg aagattcgac agtttcagtc ttgtgtgcca
     2941 aaggatggat gcgagaacct gcaaaaccac tatcatagcg gtccacaatg tccttacggc
     3001 gttgcaacaa acttggatag cggtccaatt gtaccaaacc aagtgaagcc atgatatcgg
     3061 tcatgttgca cttataggct ggtgtaacga tatcgtattc ccatgacccc aattgcatct
     3121 tggcaagagc atccttagtt tgcccgtgaa gggaaaggat ttggaattcc ttgtacatct
     3181 cttcgtcatc aatcactgga ttggctttcc aagtcgcact tccaccttct gccgttgtaa
     3241 agttcttaac ggcatggaat gagaaggaag taaagtcagc gatagaacca gaaggttgtc
     3301 ctttataagt agatcccaaa gcgtgggcac tatcagagac aatgacaata cggttaaagg
     3361 ccttttgcca cttgcttgaa gcggtaaaga agtcacgttt tttctccacg acttggaaca
     3421 aacggtcata atcgcaaaca atccctgcga gctctactgg gataatcacc ttagttttct
     3481 cagtgatagc ttgctcaagc aggtcatagt ccatctcaaa cgtatctgct tggatatcca
     3541 ccatgacagg ggttgctccc acgtgcgtaa tgacactaca tgaagccgta taggtcatgg
     3601 ctggaacgat gacttcatca ccaggtccca cttccaaaac gcgtaaaatc aactccagag
     3661 cggctgtcgc agagttgaga caaacagtct taggtgtctg tgtgtaaaga gacaagcggc
     3721 gctccagttc ttttgtttta ggacctgttg tgatccaacc agaacgcagg gtatccgcta
     3781 cttcagcaat ttctgcttct gtgatatcag gcggtgaaaa tggaatattg taatttggca
     3841 ttgatttgct ccttttatct gtactcattt tcttatgact actttatttt agtacctcaa
     3901 acacggtttg aaacatgatt ttgatgtccc caaagaaact aaactctcgg agataggcga
     3961 ggttatagcg catcttttca ggaagaacat gctccacata ggcctgatca actgacagac
     4021 ctttctccgt catttgactg atgatggtgt cctcatcctt gtagttgatg ctggctggag
     4081 aggtaattcc tgcttgcaag agcaaggttg ccatcatttc agggctatac tgctctgtat
     4141 aacgtggcac ttcaggtcgt gtaccgacaa aggacatctc acctttaagg acattgacca
     4201 actgaggcag ttcgtccaaa cggacacgtc ggatgaaatt tccaaccttg gtaatacggc
     4261 tatcgttagc agaagtcacc agacttcctt ttttatccgc atccgtcacc atggtacgga
     4321 acttccaaat cttgaaccga cagttgtact gggtcacacg ctcttgcttg taaatcactg
     4381 gccccttgct atccaacttg atccaaatgc tcaagatgag aaagatggga gaagttagaa
     4441 ccagtaagac caaggccaaa acccagtcca agcaacgctt gaaaatcagc gaacccttcc
     4501 ttttagagac aagctggtag taagactcaa cctcgcttga tttcatttcc acaggcaagt
     4561 cttcccattt cagcatgctg ttctcctttg tttttattat actatttgtc aacatttttc
     4621 attataccac aaaatggaaa aggcggtgaa agaaatctgt aatttgaagg atttccttct
     4681 agtactcaat gaaaatcaaa gtacagcttt gagattgcag ataaagctga catggtttaa
     4741 agagattttc aaagagtatc ggccatggcc cctgcctgca aactatacat cttgtgataa
     4801 gttcctccca gagccaagag ttcctcatgg gttccactct cgataatgcg tcccttatcc
     4861 aagacataga tgcagttggc gtcttgaata gtagaaaggc ggtgagcgat agcaatagtt
     4921 gttcggccct gtctcatctt cgccagagaa gcttgaacca agctttctgt ttcagagtca
     4981 atattggctg tcgcttcatc caaaatcagg actttaggct ggctggcgac tgttctagca
     5041 aaggcaagaa gctggcgctg cccagtagag aagctcgaac cacgctcgga aacaggggag
     5101 tcgtacccct gaggaagttc ttgaataaag gaatctgcat ccacaaaggc tgccgcagcc
     5161 tgaacctgct catcactgat ttcttggtac atggcaatat tggacttaat agttccatga
     5221 tagaggaagg gttcctgcaa gaccaaaccg atgttttttc tcagctcttc ttgactgaaa
     5281 tccctgatat ccacatcatc caagagaact ctccctgact ggaattcata aaagcgcatg
     5341 aggacattga taatagacga tttccctgaa cctgtatgac ctacaaaggc aatggtttca
     5401 cccttattaa cagagaaaga aatgtcatcc agaatcggat gtttaccgtc atatgagaaa
     5461 cacacatgtt caaaacggat attgccttct tggactttgg cttgcccatt ttcttgaaga
     5521 ggttcatagg tcctctcgtc tatcagggca aagacacgac ctgcagaaac catagccgtt
     5581 tgcagagttg aaaagttttg cgtcacctca atcaaggggt caaaaaggcg gttgatgtac
     5641 tggataaagg catacatggt cccgaccgtt atcccgatag aaaaaccacg gtagccaaag
     5701 taggccatca agactgcata gcctagaagt ttcagcaaac tcatggcagg tctcaaaaag
     5761 agggcatcca aggctacaga acggttggcg tagaccaagt gttcttggtt gatttcatca
     5821 aattctgcct gcaggcgctt ctcttgatta aaagcctgaa taatcctgat tccctcgata
     5881 ttctctgcca gcttactatt gatatctgac aagagacttc tggttttctc gatgattttc
     5941 actgactttt ttcgatagag attgaccaaa aggaaaatca aaggaagaaa gagcaagact
     6001 aaagccgtca aacgaaaatc cagcaccaac atggtataaa gggttgtcag aaagataaaa
     6061 actgctgaga taaagctgga taaaatccca gaaaacatat cactaatcgt ctcggtatcg
     6121 ttggtcaaac gagaaacgat agaacctgct ggcgtcttgt caaagtaaga catgcccagt
     6181 ttctccatat tggcaaaggc atcccgacga atatccctaa caatactgta agacacgcgc
     6241 gcaaagagaa gattgcctac atactgaact acagtttgta ggatgtagag accatagtag
     6301 accagcaaaa cggtaacggc tagttggtta agattgctga gatactggtc gataaagtgg
     6361 gaagccacga ggggtatgac acttttaatg accgtcgtcg ctaggagaaa actgagtgcc
     6421 aaaaagatca ggagtccata agacttgaga taagacatca agcgcttcaa tacagcccat
     6481 tgttcttgtt tattctgcat cttcttctcc tttcatttct aactgctgag actggtaggt
     6541 ttgggcatac cagccatcca tcgctagcaa gtcttcgtgc ctgcctcgtt cgataatttg
     6601 accattttgt agaactaaaa taaaatctgc atggacaaca gcactgaggc gatgggcagt
     6661 gataatggtt gtcttgtcct ttcgcgtctc cttgaggttg tcgataatcg catactctgt
     6721 cttggcatct acggcggata aggaatcatc caaaatcaag atatcagggt ttaaaatcat
     6781 agcccgactc atagccaacc gttgcttttg accaccagaa agactgactc ctttttcacc
     6841 aatcagcgta tcaaatcctt gaggcatgtc tacaatatct tggtaaactc gggctagctt
     6901 agtagcttcc tcgaccgctg aaaggggcaa gttaggattg ccaaagcgga tattgtctag
     6961 gattgaagtt gcaaagagaa actggtcctg aggaacatag cccatgagac tgcgaaggtc
     7021 tgtcagacga tagtcccgaa tatcgtgacc gtttagataa atggcgccct tatccacatc
     7081 gtattcacgc aagagaagct tgattaagga cgttttccca gagcctgtct gcccaaccaa
     7141 gcccagtgtt tgcccttttg ccaaactaaa gtgaatatcc gtcagtgttt cctcattttc
     7201 aaaagcaaag ctgtcaatgg catactccaa acgcccattt tcaataccat ccagaggaaa
     7261 ctcagggtct tgtacaggag attcctgaga caaaagattt tcaatccgct ggtaagaaac
     7321 cttccctcgc tgagtagtat taaagaggaa gccgatggcc ataagaggcc agaccagcat
     7381 atccaaatag ctgataaagg tgaccagatt cccaactgta atctgccctt cctgaaccat
     7441 caaggagcca accaaaagcg ttaaaacata ggacgaacca acaaacaaga gaaccatagg
     7501 gtcaaagaga ctatcatatt tcatggtttg caggttcttt tggaaggtta attcattgac
     7561 tgcctgaaaa gacttcaact cgtctgcctg ataaccgaaa gacttggtca ctttgatacc
     7621 tgatacggac tcctgtacct tgttattgag ttcagaaaaa gcagcttggg attcgccaaa
     7681 ggccttatga gtctttctcc ctaggcgact agtcgcatag gccatgaaag gtagggggag
     7741 aatggcaaca agagtcatct gccatgagat gctaaagagc atggtcaaca aagtcaccag
     7801 agccgtgata gaggcatcca ccgcagacat gacaccgcca cctgctaaac gagtcaaggc
     7861 attgatatca ttggttgcgt gtgccatcag atcacccgtc cgataggttt gataaaaggc
     7921 tgacgacatt tttgtgaaat gcttaaacaa gcgagaccgc atgatctgtc ccaagcaata
     7981 agaggtccca aggatataca tacgccacac atagcgcaaa tagtacatac caaaggctgc
     8041 aagtagcaag taaaataggc taagaaggag gtcctgctgg gttaattgcc ccgatgtgat
     8101 ggcatcaatg acccgcccca taaccatagg aggaatgaga ttgaggacgg aaaccaagat
     8161 cagggccaca attccgacta gataacggcg tttttctaac ttgaaaaacc accaaagttt
     8221 ttgaataatg gacataaaat ccctttctga ttgcaaatgg aaacctgagg ccaatactca
     8281 atgaaaatca aagagcaaac taggaaacta gccgcaggct gctcaaagca ctgctttgag
     8341 gttgtagata gaactgacga agtcagtaac ctacatacgg caaggcgacg ttgacgcggt
     8401 ttgaagagat tttcgaagag tacaagaccc caggtttttc ttattcataa gttacgactg
     8461 taacagcacc cttgtcatat tcagcaataa agatattggc tacattgtca tgcccttgtt
     8521 tactgaggtt atcaagcaac cactcttcgc tacgaccaat cgattccaag acatctactt
     8581 gaatcacacc gtcagtcaca actggatact taggattttc atctcccatt tgcacaacga
     8641 tgagttgccc attttgctct tgcacagctc gtttgacttg cttcatctgg aaaatccctt
     8701 ggctacgaag tttgagggat acatccgatg cagacaaacc aactgaacga caggcttctg
     8761 ggtcaatatt cccatttttg ataaggagag ttggcttacc atcaatcaaa cgtttcacaa
     8821 agtgaacatt gttgttgagc tatttgagag tcaagatcag tttgattaaa atttcgatat
     8881 aattaagtgt catttgttca catccaccag ttcaattgcg tctgtcttat acaattcgac
     8941 tttctctaac aggtacttgt ctggctcact tccgtttagg gcacgataat agcgatctcc
     9001 caccttgata agtgcgccat ctgtgtttgt ggaagtatta acatagactt ctgacttgtc
     9061 tactcccaaa tctttggaaa caacctcgat gaaatgaagt gaagtttgaa atttattgtt
     9121 agaaacttga ttgttttgat aagtcgtgat gctgaccaaa agcatagcta aataaggcca
     9181 gagctgaaat tatgaccaac tcacgaaatt tggttccttt tttatcatgg tatgccttga
     9241 aagcaaaaaa aactgtcaca gctaataaaa caatcccaaa gccaaccatg ataccatttt
     9301 gctgaccgat ttggctgagt acatagtcat aagagtagaa tttcatgtat tttcactccc
     9361 tttcataaaa tcattcttaa ttataaacga aagagagtaa tttttcaaac catacgttgg
     9421 ggaaatagat ctttttttgg tataataaaa tctataatct gaatgaaaaa ggtaacttta
     9481 tgaaacttat ctcatggaat attgattccc taaacgctgc cctaactagt gactcagctc
     9541 gtgccaaatt gtcccaagaa gtcctacaaa ccttggtcgc tgaaaatgct gatatcattg
     9601 ctatccaaga aaccaagctt tctgccaaag gacctacaaa gaaacacgtg gaaattttag
     9661 aagaactctt cccaggctac gaaaacacat ggcgctcttc ccaagagcct gcccgtaaag
     9721 gctatgctgg aaccatgttc ctttataaga aagaacttac gcctacgatc agttttccag
     9781 aaatcggtgc cccttctacc atggacttgg aaggccgtat catcactcta gaatttgatg
     9841 catttttcgt aacccaagtt tacactccaa acgctggtga cggtctcaaa cgcttggaag
     9901 aacgccaagt ctgggatgtc aaatatgcgg agtatttggc tgaactagac aaagaaaaac
     9961 cagtccttgc gaccggtgac tacaacgtag cccacaatga aatcgacctt gcaaatcctg
    10021 ctagcaaccg ccgttcacct ggatttactg acgaggaacg tgctggattt accaaccttt
    10081 tggcaactgg atttacagac accttccgcc atgttcatgg cgatgttcct gaacgctaca
    10141 cttggtgggc acaacgcagc aaaacttcta aaatcaacaa tacaggctgg agaatcgact
    10201 actggctcac aagtaaccgt atcgctgaca aggtcactaa atctgatatg attgactcag
    10261 gtgctcgcca agaccataca ccgattgtct tggaaattga cttgtaagga ttttctatat
    10321 ggactacaat gcggtcattc ccgagtttct tgtatcgaat atcgaacagt cccggtcctt
    10381 ctactgtggt ttactgggtt ttaggattga atactaacgt ccagaagaaa actttctctt
    10441 tctcttgaag agtgtcaact aatgttagag gagggaacaa aggatcaact agctgaactg
    10501 acctacccct ttggtcgtgg agtcaatctc tcctttggca taaaggatgt tccaaaactc
    10561 tatcaaaaag tgatggaggc aaactatcct atttatcgtc tcttgaccaa aagaaagttt
    10621 cgtgtcagtg atccctatat ctatcctcat aaatttgcag ttttggatcc agatggctat
    10681 tttttaagat ttagcgagta gaataataaa aattgggaaa agtctttaaa gttagaaaaa
    10741 ggcatattgt caggtgtcta aaaacttgat agtatgcctt ttatgatgga aaaatcttct
    10801 aatactcttc gaaaatctct tcaagccacg tcaacgtcgc cttaccgtag gtatatgata
    10861 ctgactctgt caattctatc cacaacctca aaacagtgtt ttgaggttgt ggatagcttc
    10921 ctagtttgct ctttgatttt catttagtat aaatgttagc ttacaatgga gtttttgttc
    10981 accctctctt ctttttgttc gctttttaaa ccgaaacact aacaaaatag ttgatttata
    11041 aagttattta gtgtaaaata aaaacattgg ctcaagccta tttaaaattg aatatcgttt
    11101 tacttgttta tgtcgtgaat tggcacgacg tttctacaag gtgccggaac acctaacaat
    11161 aagtaagtca gcagtgagat atggccgttt ttgccatgtc tatttagggg acttacttag
    11221 agattaagta ggtctttttt gaatattttt aatatttccc ttaattagtg cggacgggag
    11281 gtgaaattat cctgtgaata atttcaggaa tccagggaat ttcatcccta atttttgagc
    11341 ccctagctct ttcatttcta ggcttaggct aaaatagttc cccgaactat tttactctca
    11401 aaaatcccgt catagacaat aacaagatta ttgtctatga taccactaca acgggaaata
    11461 tttgataaga ctcgcttcgc tcgtaatagt tttatcaaat atatagtatt agcaaaaggg
    11521 atcactatag aaatatttaa acaacaagta aattaacttt tagactttag gaggtcttat
    11581 gaaattatta gaagagcgca tcctcaagga tgggcatatc ttggatgata acatcctcaa
    11641 ggtagattcc tttttaaccc accaagttga ctttagcttg atgcgagaga ttggtaaggt
    11701 ttttgcggaa aaatttgctg ctgccggcat taccaaggtc gtaaccattg aagcgtcggg
    11761 tattgcccca gccgttttta cagctgaagc cttaaacgtt cccatgattt tcgccaaaaa
    11821 agctaagaac atcaccatga acgaaggcat cttaactgct caagtctact cctttaccaa
    11881 gcaggtgacc agcactgttt ctatcgctgg aaaattcctc tcaccagagg acaaggtttt
    11941 gattatcgac gatttccttg ctaatggcca agctgctaaa ggcttgattc aaatcatcga
    12001 acaggccggt gccacagtcc aagctatcgg tatcgtgatt gagaaatcct tccaagatgg
    12061 tcgtgatttg cttgaaaaag caggctaccc tgtcctatca cttgctcgct tggatcgttt
    12121 tgaaaatggt caggtcttat ttaaggaggc agatctctaa tgcaaactca agaaaaacac
    12181 tcgcaagcag ccgttctggg cttgcagcac ttactagcca tgtactcagg atctatcctg
    12241 gttcccatca tgattgcgac agcccttggc tattcagctg agcagttgac ctacctgatt
    12301 tctacagata tcttcatgtg tggggtggca accttcctcc aactccaact caacaaatac
    12361 tttgggattg gactcccagt cgttcttgga gttgcattcc agtcggtcgc tcccttgatt
    12421 atgattgggc aaagccatgg tagtggcgct atgtttggtg cccttatcgc atctgggatt
    12481 tacgtggttc ttgtttcagg catcttctca aaagtagcca atcttttccc atctatcgta
    12541 acaggatctg ttattaccac gattggttta accttgatcc ctgtcgctat tggaaatatg
    12601 ggaaataacg ttccagagcc aactggtcaa agtctcttgc tttcagctat tactgttctg
    12661 attatcctct tgatcaacat ctttaccaaa ggatttatca agtctatctc tattttgatt
    12721 ggtctggttg ttggaactgc cattgctgct actatgggct tggtggactt ctctcctgtt
    12781 gcggtagctc cacttgtcca tgtcccaact ccactctact ttgggatgcc aacctttgaa
    12841 atctcatcta ttgtcatgat gtgtatcatc gcaacggtgt ctatggttga gtcaactggt
    12901 gtttatctag ccttgtctga tatcacaaaa gatccaatcg acagcacgcg ccttcgcaac
    12961 ggttaccgcg cagaaggttt ggccgtactt ctcggaggaa tctttaacac cttcccttac
    13021 accggatttt cacaaaacgt tggtttggtt aaattgtcag gcatcaaaaa acgcctgcca
    13081 atctactacg cagctggttt cctggttctc cttggactgc ttcctaagtt tggtgccctt
    13141 gcccaaatca ttccaagctc cgtcctcggc ggtgccatgc tggtgatgtt tggttttgta
    13201 tctattcaag ggatgcaaat cctagcccga gttgactttg ctaacaatga acacaacttc
    13261 cttatcgcag ctgtttcaat cgctgcaggt gtcggtctca acaacagtaa tctctttgtc
    13321 agcatgccga cagccttcca aatgttcttc tcaaacggaa tcttcgtagc cagcctactc
    13381 gctattgtcc tcaatgccgt attaaatcat aaaaagaaat aagaaaaaga ggtgcgagcc
    13441 tcttttttgt gtgacaaaat aaaaacatct acctattcat atatgctagt gtcatagtcc
    13501 tgaaaatcat ctgcatcaag aacaatttca caactcttat acttttctct tacaagtcgt
    13561 tcggcttcat ctggattttc agcctctata cttactaaac gtgataaagt ttctgtaatt
    13621 tctactgtat actttttcag ttttagattg ctttccaata attcacctat taacaataat
    13681 ctatgatagg agctatgtag ccactctggc aatttatctc ctacttccca cccatatttt
    13741 tccttcatca aagctagttg tttttcgtca ccaaatttca tattttcaga taaccatctc
    13801 acacaatcgg gatctgataa ggtacagacg attaaactta ggttatcgta acggctttca
    13861 gtttcataat caggccagat gacttttaag aaatcataca cctcttctga tgcaacaagt
    13921 tgtttcgttt tcataatttc cgatactttc ctctaccttt aaattctatt atttctttgt
    13981 ctcttaatat ttgaagctgt tgcctaatct tttctttgat atgattgttc ttaacaaaga
    14041 tattttttag gtcactttca aaacgataca tatcttcaag ggtaaattct gaaccctcta
    14101 tcttatctat acaatttaga atttctattg tccaacctct tgatgacaga gagctcttcc
    14161 ttaaaaataa accttgctta aattcttttg taactttttc tggatctcta acttgtccat
    14221 cttgcacaag aaatatcctt cctttagaag gtacttgtga taaatcaatg ttacaaccaa
    14281 tccaacctgc tcgtctagca gttggtgcaa gtggttttct ttgaataatt gatttcggtg
    14341 taacaaattg cttcggaagg acaagaaagt tatttacctc aaaatttttt gtgtaagtta
    14401 aaaaaaagaa attaggatta ttatctgcct gcacacgctt catcatcgtt gcataagcac
    14461 catcattgat tgttgatgaa aaatttcctt ttttgctctt tagttcaaac tcctcactac
    14521 aatgattaca gtaaaaatct gctacaggcc gattattttc aaaatgattt aagggattgt
    14581 tcccacaatt agggcaataa gactgacgat aaacccaatc ctccgttaag atacgtgcct
    14641 tttggctatt acttttatag gtttctacta attctaaatt aaagtgtaat tccatttaaa
    14701 aattcctcac taatatgaga actcactcga tataagcaat taaaatctac tataactagt
    14761 ataccacata tatgtaccta ttacatttgt atttcaccac tctcttttat atttccttct
    14821 aacatatctc ataaattaaa aatgagtgag ataaatttat cccactcacc tttctttata
    14881 tccttacccg tctttcctca gacctttggc ctgtgacaaa catggtaaag gttgctttac
    14941 agacatttct gccctcttga ttggtgatat ccacatccat cacacaggtt gtacgacctt
    15001 gatggacaca ttctccttta atggtcaata catcgtcgag ttttcctgcc ttaaggtagt
    15061 tgatagagga ttggagtgtc accccatcaa gtcccagcga gataaccacc aaaccactga
    15121 tttggtcaca tagcgtgaat agatagccac catgggcatt gccatagtag ttgagcgacg
    15181 agttcactac tttggtcgtc accacaacgt gaccatctct cattttttca atttcgtaat
    15241 tttcaaaggc agatatagcg tcaaaatgaa agtctttcat cgtttcctcc tgatatttca
    15301 aacgacacta tgatactact ttttatagga ctgtgcaagg agaaagctcc tagtctagtg
    15361 aaattccgac ctgttccaca aagcgcataa aggctgtctg cccttgttct gtctgcttgt
    15421 agactcctgc atcctcaagt acacgcacaa agatagcacc cacagagtcc ttgacgattg
    15481 caagggcttt ttctttatcc gttaggtctg gatgttggga tttgagttgg tctgcccact
    15541 cctgatgata atcggcaact gtaacagctt ctcctacaag atagctagcg acttgctcca
    15601 cttcttcttt cagacgtggt ggcaagattg ccaagcccat gacctcaatc aagccgatat
    15661 tttccttctt gatatgttgg acatccttgt ggggatgata gatgccatca ggatgctctg
    15721 ctgaagtctg attgtctcgc aagaccaagt ccaactcaaa ctgtccatca cgcttacgcg
    15781 ctattggtgt gatggtgtga tgcggcgtcc cgtctgtctc tgctaaaatc tgcacactag
    15841 aatctgaata ctgacgccat tcctggaaaa tcttatcagc taagttaatc aaatcctctt
    15901 tggaatccga agtcaagcgt aggacagaca ttggccactt gataattcca gccttgacct
    15961 gctcaaaacc agcaaatcgg aaggtctttt gcaagggagc caattccata ggaaatacgt
    16021 gacggcctcc ctgataatga tcgtgagtta gaatagagcc ccccacaatc ggcaggtcgg
    16081 cattagatcc agcaaaatat cctggaaact gctctacgat agccaacaga cgttcaaaac
    16141 tctgacgact aatggccatg ggacgatgct ggccatctaa aaagatacaa tgctcattaa
    16201 agtaagcata gggcgaatac tggaaccccc attcctgacc aaccatttca aaacggataa
    16261 tccggtggtt gctgcgagct gggtggttga cccgaccatg gtaaccctca ttctctaaac
    16321 aaagttgaca ctgaggataa ttactagctt gcaccaactt ggctaccgca atctctttcg
    16381 gatccttttc aggcttagag agattgatgg taatttcaag ttctccataa tcagatggaa
    16441 cacgataagc gatattttta gcaatggcct tgagtttgat gtagtcattt ttctgactga
    16501 gttgataaaa atcctctatc gcttgctcag gagagtaggc ataggcttcc cagaagtcac
    16561 gattgacctg actcggacaa ggagtcacca agtccatcag ttcagtacca aggatttcac
    16621 gcgcagtctg actatcctca atcgtctcta atcgaacggc ttcctcaact agctgggcct
    16681 tgaggtcaat caatttatcc agattggtct caacttccaa aacaccttct cccactcgtg
    16741 ccaaaacacg attggtcagg tagattcgat ccatttcctc aaatgaactt tcagaaatga
    16801 catgtgttac aaatttatct actaaggtca ctatagagcc tccttttgac tagtcaagga
    16861 cgcgagtgcc acctgcaact tcagcgatat agaagcttgg agcgtatcca actacttcct
    16921 cgtagtgttt gcctacagct tccttaaagg cctcaacagt atctttttga accaaggcaa
    16981 tggcacagcc accaaaacca gcccctgtca tacgagcacc gagaactcct tcttgtgccc
    17041 aagctgtgtg aacaagggta tctaattcca aaccagttac ttcataatca tgctccagag
    17101 aaacgtgtga cgcattcatc aaacgaccaa atgtttccaa atcacctgct tgaagggctg
    17161 cttgagcttt aagggtacgt tggttttcaa gcacagcatg gcgagcacgt ttcaaacgat
    17221 tttcatcttt aattagatag ctatattggt caacggccca ctcgtccaat tcacccaggg
    17281 tctgaatatc caaggcaact tgcaattctt ccactgcttt ttcacactca gcacgacgtt
    17341 cattgtattt agagtccgcc aattcacggc gtttgttggt gttcatgata acaacgacat
    17401 tgtccttcaa atcaagtggc accaaatcat actctaaggt gttggtatca aggtaaatag
    17461 cacgttgatc agcccccata ccaatagcaa actggtccat gataccagag ttgactccga
    17521 taaagttgtt ttctgtttgt ttgccgattt taaccaaatc gagacgctct aattttaaat
    17581 caaagagatg ctctgccacg actcctgtca agagctccaa ggatgctgaa gaagacaagc
    17641 cagcaccatt tgggatattc ccataaacat aaaaatcaaa acctttgtca atcacgtgcc
    17701 cagcttcttg caagaaatga agaacccctt ttggataatt ggtccagttg tgctcttttt
    17761 caaacttgag gtcagcgaga ggcacttcga taatgccctt gtcctcaaag ttagctgagt
    17821 agaaacgcaa gacttggtcg tcacgcttac gagctgcacc gtaagttccc aaggaaatag
    17881 cagcaggaaa aacgtgccca ccgttgtagt ctgtgtgttc accaatcaaa ttaatacgac
    17941 ctggtgaaaa gaaggtttgg tctgcttctt gaccaaaaac agcaagaaag tctttgcgaa
    18001 ggacttcagt agtaagatgt tgtgccatat gaattctcct ttgactgtcc gttaagccat
    18061 cttaacgtgt aatcgttttc ttctattgta tccaaggaat ttcccaaggt caatcctttt
    18121 tactaaaatt ttagtaaaac atcagtaaaa agaagaaaaa tatgatatac taacctaatc
    18181 aaggaggatt tatggctacc ttaaaagaca ttgcacagct agcctctgtc tctatcgcga
    18241 ccgtatcccg tgtcctcaac cgcgaccaga gcctatctgt tacagaagaa accagacacc
    18301 gtattttaac cgttgctgaa gagctgggct acaccaagca cctcaagaca ggcgagtccc
    18361 ataaacccaa gcaaaagatt gccattattc aatgggtcag cgaacaaggg gagctagacg
    18421 acctctacta ctaccagatt cgcctaggaa tagaaaaaag agcccaagag ttggactacg
    18481 atatcttgcg ctattttaat gaccatcctt ttaccctaag cgaggaagtg attgggattc
    18541 tctgcatcgg aaaatttagc cgagctcaga tttctgcctt tgaagaatac caaaagcctc
    18601 ttgtatttct agacagcgat acactttccc tgggacatac ctgtattatc acggattttt
    18661 acactgctat gaaacaggtt gtcgattatt tcctcagtca agggatggac cgcatcggga
    18721 ttctaacagg gcttgaggaa acaacagacc aagaagaaat cattcaggac aagcgtctag
    18781 aaaacttcaa aaactacagt caagcgaggg gaatctatca tgatgaactg gtctttcaag
    18841 gaagatttac tgcccagtct ggctatgact taatgaagga ggccattcag agcttgggag
    18901 accaacttcc gccagcattt ttcgcagcca gcgatagttt agctatcggt gccctccgtg
    18961 ccctccaaga agctggaatc agcctgccag atcgcgtcag cctcatttca ttcaacgaca
    19021 ccagtctgac caaacaggtc tatcctcccc tctctagtat tacagtttat actgaagaaa
    19081 tggggcgagc aggtatggat attcttaaca aggaagtcct ccacggtcgc aaaattccta
    19141 gcctgaccat gctgggaacc agactgacat taagagaaag taccctaaat caagaatagg
    19201 ataacataaa aaacgaatag agttctaaaa ctcctattcg ttttttattc gattacaatc
    19261 atagacttaa ttgtcttacg ttcatccata tctttatagg cttggtcgat atcttccagt
    19321 ttataacttg aagtaaagac gcgacctgga ttgatatcac catcaaggac ggcttttagt
    19381 aaaaattgct tatcgtatgt tgtagcagaa gctgccccac ctgctacaga gatattttgc
    19441 ataaatgtcg aaccaagagc acgattatta tagtgtggga ctcctacaaa gcccatacgc
    19501 cctccattat gaagaacacc tagcgcctgt tctatagcag cctccgtacc aacacattca
    19561 agtgctgcgt ctgctcctcc gccgaggatt tcacgcacct tggtaattcc ttcttgacca
    19621 cgttctgcaa caacagctgt cgcacctgac tccatagcca tcttttgacg gtcttcatga
    19681 cggctcataa ggataatttg tgatgctcca cgcatcttag ccgcgatgac agcacattga
    19741 ccaacagccc catcaccgat aacaacaacc ttgtcccctt tttgaacatt tgcaacacgc
    19801 gctgcatgat agcctgtcgg catgacatct gcaagagtca aaagggactt gagcatccct
    19861 tctgtataat cagaaggttg accagggatt ttaaccagcg cccagtttgc atagtggaag
    19921 cgaatatatt ctgcctgaaa atcacccccc aaattattac caatatgatt gtcgcaagaa
    19981 ccgtcaaatc cagcaagaca ggcatcacac tcaccacatc catgtgtaaa agggacaatc
    20041 acaaaatcac ctggtttcac cgtcgtaatg gcttccccag cttcttcaac aatcccaatc
    20101 gcttcgtgtc cactattttt atgtccagct ttcgtttctg gattacggta cctccataaa
    20161 tctgaaccac aaacgcacgc acgaaccaca cgaataatca catcatccgc ttctattatt
    20221 tgcggacgtt caatgctagc aagtccaacc tgacctgcct ttgtatatac tgctgatttc
    20281 atttaaaatt ttccttcctt ataaagttta attttgagat ttaaacgatt taaagctgtc
    20341 tgtagctgag acaagcgctc ctctaatttt tgcttttcct cttctaaaat accaagcctc
    20401 tcctctctcg tttcatctcc cttttggtag agcgacatat agtcaactaa actatctaca
    20461 gagacacccg ccgaacgaaa acacttaata aattccagcg cttcgatatc ctgatcttga
    20521 aaatcacgaa tcccagtagc agtacgagta atcggtggca caagaccaac ccgttcataa
    20581 taccgaatcg tatccgctga aattcccaac aaatcactgg cagatttaat attcacacgc
    20641 taacctcctc tcatttcatt cactgaattc acgctattct aactcactta tcttattata
    20701 taactttgag ttgactccaa gtcaagcatt ttgtatctaa tcgaaaaatt tttatcatta
    20761 acaatcatat atctggagtt tatacaatgc tcttctctta ccaatttttg aaatatttaa
    20821 ataataaaga aattactcta ttccaaataa gatagtaccc aagtattttt cacttttttc
    20881 taatgttgat gctcataact ccgttccaag tcacacacct ttcgcttatg ggtttggtga
    20941 gtttctaggt cagcatcaat ttcaatggta atattttgaa aaccacaatc ttttaggaaa
    21001 attcgaatag actctttaca agtttccata tgttccattt cttttagaca aacatgtaca
    21061 atggcatttt tttccaaagc atccatagtc cagagattaa gctgattaag gctggccaca
    21121 ttgtccaatc gctccaggcc actctttact tgcttgatat caagaccttc tggcacagca
    21181 tccaagaaaa tcttgagtgt agaccaaaaa cgtggaaggg cttttgaaag aataaagaaa
    21241 gaaatgacaa gggacaaaag aggatctagg atataccagt ccgtaaatcg aagaacaatc
    21301 gccatcagga taacagctac ccaccctagc gtatcttcca gaaaatgcaa actcagaata
    21361 gactcattct ttgtctttcc cttaccaacc accagactcg ctaacagatt gatagtaatc
    21421 gcaataattc ctaaccagag aatcccctca tcattgactg gttgcggatg caaaatcttc
    21481 gtgacatttt ccaaaatgac tagaacagag cccgttacga gaatcacagc tgttaccaag
    21541 gctcctagca ggctaaaccg cttatagccc aaggtgtact gattgtcttc ttcacgattg
    21601 gagattgttt ctagaaaagc tgatattcca attgcaatcg catctcccaa gtcatgcaca
    21661 gagtcagcaa gaacagcgct agaaccaaat actccacctg caataaactc aacaatggca
    21721 taagtcaaat ttaagaaaaa agccacccaa acagcatatt ttgccttcat atttctcatt
    21781 cctttgttat aatagattta tgaacacctt gttcattatc attatccact aaaactcaaa
    21841 gaaaggatag aagcaaaacg tcagctttat tcagttctga acaatttgcc ttaagtgtcc
    21901 atatgactaa cattgaccgc cgtatcagca aaaccaaaaa agccatctat caagctttta
    21961 tacaactttt gaatgctaag ggctacgagg ccactactgt tcaggatatc attgatctcg
    22021 cagatgtggg aagatccacc ttttactgtc actatgaaag taaggagctt cttctggacc
    22081 agctctgccg ctacctcttc catcatcttt ttgaaagaga gcaagccatt tcaaccgagg
    22141 actacctcgc ccacctcttt ctccattttc ataaaaacca agaccatatc accagtctgc
    22201 tattttccaa aaatgactac ttcctccgtc aactccataa agagctagaa caccatgtct
    22261 attccgtgct agctgataat ttaaaagaag cccaccctaa tatgcctact tcttacctcc
    22321 aacacttggt cgtgtccaac tttatcgaga cattgacctg gtggctcaaa aaaggtcaag
    22381 atttcacagg ccaggaagtt gtccaatttt atctagacct tctcattcct aaaaattgaa
    22441 tatagagtaa agctcagttg tcttatttct aggttactga gttttttatc ttttcaacaa
    22501 caaaagagga cccgccgatc ctctttttca tactataaat ccttgattat caactatatc
    22561 tgttttaatc gaaatctcaa aacagcactt tcaaacatct tttcctagtt aagtaaatca
    22621 gtattctgct tagctgcctt gctccattga taccaaccaa ctagactgtt aatgagataa
    22681 attagatatt tcccttgaat ttgcaggctt tctccccacc agagatagat tgaaaaaaca
    22741 ttggtagccg cccagaatat ccactgttca cggtaaacag ctgtcatgag gatttgccct
    22801 accccattgg ttgcatctgt gattgaatca cgatagggac gattggcacc aatagactga
    22861 taaatgaagc caaaggccaa ccaccaaagc acactaatgg aaagatactt tgtccagccc
    22921 ttgccgtcca gtttacgcgc gacaaactcc tgcttttcct tcttaaactg tgcctgataa
    22981 atccaaacta gaagtccaat tggctgcatg actgtgaagt aaagtgtcgt cagcacctca
    23041 ccataaaagc ctttctgtag ggccaaaata aggtaaataa cagagttaat caagccaaaa
    23101 agataattac ttgctcgacc ttccgataca aagattacac agataatccc tgtcaagcta
    23161 caaatcatcc caatccagtc aacaatacga tgttcgtaaa ccaactccag ccagagagga
    23221 aaacttccta aaaccagcaa ataaatccac tgggcaaaac tacgatgggc aaagaggtca
    23281 tcccagatag ccttcatagt tcctgaaaat cctaaatcag ccatagccgc aaccatacga
    23341 cggtaaccac ctgacatttc acctagggtt gttttgatat tttcaatttt cttttgcaaa
    23401 taagtatgca tcatttctcc ttttgttttt aaagagccgt gtctggatag actttcggac
    23461 gcaacgctct attagataat gaactgccta tacacaagat ttctaacctt agtcgacatg
    23521 agctaaaacc tcttatttgt taagtagttc acaaaatatt atacacctat tttatgaata
    23581 gtcaactgtc tttacagtaa aattttagaa aatcatgaaa attttctctt tctttccatt
    23641 ttaagtgaca ttcagtcatt ctcacatcaa aaaagcccag acgaaattgt ctgagcattc
    23701 ttttatctag tcgtttaagg aagttgagtt cagtatgttt aaagtctctg tcccatcatt
    23761 tcttcaacaa accttgttct tggagaaact ccttggctac ttgctttgct gacttgcctt
    23821 caacaccgac ttggtagttg agctggctca tctggctttc tgtaatctta ccagccaatg
    23881 tattaagaac tctttccaac tctgggtgtt tcttgagaag agcttctttc atgagtggag
    23941 ccccttgata aggtgggaag agttgcttgt catcttccaa gacctgtaaa tcataacgct
    24001 ccaattccgc atcagtcgaa taggcatccg tgatttgaat atcccctgac tgaattgcct
    24061 gataacgaag agctggctgc atagtcgcca cattgagatt gagaccatac attgattgca
    24121 agcccttatt tccatcttca cggtcgttaa actcaagcgt aaagcctgcc ttcagctgcc
    24181 cttccacttt tttcaaatcc gaaatggtct tcaagccata ttcttgagca atctttttcg
    24241 gaacagctac agcataggta ttttgataag acataggttt gagataggct agatgatcct
    24301 gcttggcaat gccatcacgc gccacttgat aaacctgctc tggctcatga ctcaccttgg
    24361 gtgatggttg aagcaaactt tcagtcaccg taccggtaaa ttcaggataa atgtcaatat
    24421 cacctttttt cagagcttca taaaggaaac ttgtcgtccc aaaattcggt ttaacagtcg
    24481 cagtcatgct ggtattttct tcaatcagca acttatacat attagccaaa atttctggtt
    24541 ctgggcctat tttcccagca ataatcaagt tttccttctc tttttgagcc aaaagagctg
    24601 gactataaga cagacccagt aataaagcca ccaaggcaaa accagagaaa atcgtccgca
    24661 atttcgcttt ttccattact tttagtagga agttaaaggc aatagctagc actgcagaag
    24721 aaagggcccc aatcaaaatc aaactggcat tattacggtc aattcccaac aggataaagg
    24781 aacccagtcc ccctgcccca atcaaggtcg ccaaggtagc cgtaccgata atcaagaccg
    24841 ctgccgtccg aattccagac atgataacag gcatggcaag tggaatttca aacttcttga
    24901 gacgctccca tctggtcatc ccaaaggcaa tcccagcctc ttgaagactc ggatcaattc
    24961 ccttcagccc agtaatagta ttttgtaaaa tcggaaaaat cgcataaatc actagagctg
    25021 tcaaagccgg caaggtccca attcccatca aagggataaa gagccccaac aaggccagag
    25081 acgggatggt ctggaaaata cctgcaatct gcaagaccca gtcggccagc ttctcatgat
    25141 agcgaagaaa aacagccaag ggaatcgcaa gcaaaatagc tagtaacaag gtcaaaagtg
    25201 acaactgcaa atgttgagat agagctgtca accaatcact aaaacgatcc tgaaaagttg
    25261 caattaaatt agtcatgaac actacctcca aacaagtctg ctacaaagtc tgttgcaggc
    25321 acttttaaaa ttgtctcggg attcgctacc tggcgaattt ctccatcctg caagacagca
    25381 atacggtccg ccaacttcaa ggcttcatcc gtatcatggg ttacaaaaat cgttgtcatc
    25441 ccaaactctt tatgcaattc ttttgtcaga acctgcaact gttttctcga aatagcatcc
    25501 aaggccgaaa agggttcatc catgaggaaa atcttgggct gaccaatcat agctcggaca
    25561 ataccgaccc gttgctgttc tccaccagat aattcactag gtaagcgatg cccatactcg
    25621 gctactggta aaccaacctt agccaaaagc tcttctgttt tcttcgtaat ttcttccttg
    25681 ctccacccct tcatttcagg aatgagagca atattttccg caactgttag atttggaaaa
    25741 agagcaatag cctgtaaaac ataaccagta gaaagacgaa gttcacgctc atcatagtct
    25801 ttgatgcgct tcccatccat ataaatattt ccatcagttg gttccaaaag acggttaatc
    25861 atcttgagca tggtcgtctt acctgaccca gaaggcccta ctaaaaccat aaattcccca
    25921 tcctcaatct gtaagttgac atctctcaag acatcctttt ctgtgtagcg cagtgctaca
    25981 tttttgtatt caatcattct ttgtcctcaa tttaaaactt ccctcgatta gtcaagtctt
    26041 ctaccttagg catgacttcc ttattatccc aatgctccac aattttcccg ttctctaaac
    26101 ggaagatatc gtactgggca taagcaacgc catcaatctg agtctgacca tagctaacca
    26161 catagtttcc ttgtcctaag agttggaaaa caaagtcaaa agtgacacta tattcagcca
    26221 catagttttt ataagcagca cttccttgtc caatctcatg attatgctga atcaaatcgt
    26281 ctgccacata atcactccac tgctctagct ccccattttg gaaagtttct gtcaagaaac
    26341 ggcgaaccag ctttttattt tctgctttct tgtccaaatc cttgatttca aaatctccaa
    26401 aaatttgatc tagttggtca ttttcaggtg ttcgatagta gtcaatgaca tcccaatgct
    26461 caacaatacg accattctca tccgcacgga aagtatccgt cgtcacccat tgagcttctc
    26521 caccattcag atattgatga acatgaacaa agaccagatt gccatcctca atggtgcgga
    26581 caatcttaat ctgacgctct ggatgacgct caaagaaatc tgcaaagaag gctgcaaatc
    26641 cttctttccc gtcaggaaca cctgtcgaat gttggatata ggtatcccct acagactggg
    26701 cttgagcctc agcaactcgt ccgtcttgaa tggcatggat gtataggttg tgagcatttt
    26761 tcacttgttg tgacatattc taaacctcat tttccttctc tttcagattt gccaaaattc
    26821 tttcttgaaa accttcaaat tggtgaattt cttcctctga aaatcctttg taaaagatag
    26881 tatccaattt ctgactgaca cgatgcccca cttctttctg gaacttgcct aactccgtta
    26941 aaactaaata cttcttacgc ttgtcttttc cacacggact aacaattaca agcttttgtt
    27001 cctctagctt ttttatcata gtcgtcagcg tattattcgc aagcccagtc gcaagcgcga
    27061 tatctgtcgc agttgcgcag ccagtttcac tattccataa aaccgctaaa atcttgccct
    27121 gttcacccct ataaagagcc tcaggatctt gactcagtaa cttttgaaaa atccgcccat
    27181 tcaacaaacg aatatgatgg gctagcaaat gactatcttt cataacacct ccaatttatt
    27241 tcgatatcga aatgaataaa ataattgtaa cactcatcgt tctaactgtc aactatttcg
    27301 atttggaaat aatttttgat aattattcac accaccatac tccggctcaa ctaactttta
    27361 acgagagttt ctaaattcct tcgtcctcca gtctacaaaa gccttccatc cgtactatcc
    27421 tattttttat gaggggacac atttttccta tcagatcatt tattttaaag atagaagtaa
    27481 atcataattg cttccatctg ttcttttata gtatattgaa gttggactag ggcactgtat
    27541 cttctaaaac attgatagaa agcgatttga atttcccaat caatttgttc gtatttatag
    27601 cattttgaaa ctagaatagg acgccatgac tgctaaaaga tttctataaa ttcatttgat
    27661 ttccctaatc aatttgttca tatcttattt cattccgcta taatttcacc ttaccctatc
    27721 tttttcgtag cacccttcaa acagcctatc ccctaccgtt tgatgattcc tcacttcact
    27781 ccacttccat tacagaagtt tcttcactac tatggtctcg gctgacttct catgattcct
    27841 tgttactact atttgaacgc tcacgagata ggtcttataa aaaatgcttt gatccacaat
    27901 ggaatcaaag cattttaaaa agttcctcat acataagcgc agaagtcgca gttcctctgt
    27961 acttggcttc ttctcttttg acaaagcgag ccaagttgag caactcaggt gctggatgtt
    28021 tgggatttag gagcaattca cgattgacca ggcttgagag acgaactgcc tgcaattgct
    28081 catttgtagt aggcagtttt ttagtagtct ctaggagagc agcaactaaa tcttcactca
    28141 aatcatgtcg agcatgattg taaagatctt ttataaggct ttctaggttt ggttctacca
    28201 tccctaccac ctcccttatg gtttaataat gtttaatcaa atcaaccgtt gaacgatcca
    28261 atttcttcac caaggcttgt aagaaagctt gcgcttctag gaagtcatcc attgcataga
    28321 gggtttggtg agaatggata taacgagcgc agacaccgat agttgttgat gggacaccac
    28381 catttttcag atgagctgca cctgcatctg ttccgccttt accacagtag tattggtact
    28441 tgataccagc ttcttcagcc gttgtcaaaa ggaaatcctt catccctggg agaagcaagt
    28501 gacctggatc atagaaacga atcaaggttc catctccaat cttgccttga ccaccgtaga
    28561 catcacctgc tggtgagcaa tcaactgcga ggaagacttc tgggtcaaac ttggttgtag
    28621 aggtatgagc gccacgcaga ccaacttctt cttggacgtt agaacccaga tagagttcat
    28681 tgccgagttt ttgacctgat aaagcttcag ctagctcgct taccatgagg acaccgtagc
    28741 ggttatccca agcttttgag atgatatttt tttcattggc tgtcaaaatt gcagaactat
    28801 ctggtacgat ggtatcacca ggacggatgc caaaactttc tgcctcagcc ttgtccgcaa
    28861 aaccaccatc aaaaacgata tcggcaatgg ctggcatggt tggtcccccc tttccacgag
    28921 tcaaatgcgg aggaacagaa cctgaaatca caggaatttc atgaccatca cgagtcaaga
    28981 gtttgaaacg ttggctgcta accaccatgg ggttccagcc accgatttct acgacacgga
    29041 aggtaccatc tggcttgatt tcgctgacca taaaaccaac ttcgtccata tgagaagcga
    29101 ccaagacgcg cggtgcatcc acagcttctg aatgtttgat accaaaaata ccacccaagc
    29161 catctgtcac cacttcatcc acatgcggtg tcaacttttc acgaagataa gcacggacag
    29221 gcgcttcatg acctgagact gcagcaagtt ctgttacttc tttaattttt gaaaataatg
    29281 ttgtcatttc agttccttct ttctttcatc cattttacca ctttttatag gagaaggata
    29341 gtgggaaggt ggatttctaa gttagtatct tagtcctgct ctatcttaga aaaggatagt
    29401 attctcttgc atgtagtgca aaatctagta aacattccaa aattaactcg aatatttatt
    29461 tccaaacaaa aaaacaatac accatcaaag ttgtttggat ttttcatgaa atttacagaa
    29521 aatagttgac ttccctttct tctttcttta aatatatagt tggttgagtt tggaatagta
    29581 cgctgtagct gctgaaacat ttctagaaat taatttgact ttcctaatag agttgttcat
    29641 atcttatttc aatttactat agtacaaaac tagaaaagga aaaaatcatg accaaaacaa
    29701 ttcttgttac aggtgggact agctacattg gctcccatac cgttaaagct cttttaaatg
    29761 ctggctataa ggtgcatgtc ctcgataatc tctccacagg aaatcgagcg gctgtggata
    29821 gtcgtgctag ctttaaagaa ctggatgttt atgatgctag cgccttaaag gcttacttag
    29881 aagaaaatca gattgatgct gtcctccatt gtgcaggtga aattgttgtg agcgaaagta
    29941 ttgaaaatcc aagtaaatac ttcactgcca acgttgctgg tatgaaccaa gttctcaaag
    30001 tcttatctga agttggcatt caaaaaatca tgttctcttc gactgcttct ctctatggaa
    30061 ataactgtat tgacaagccg gtgactgaag ataccctgct cgaccctgtc aacccttatg
    30121 cagagacaaa actgatgggc gaacgaatga tttactggat ggccaatcgc tacgactgga
    30181 aatatgttat tttccgttac tttaatgttg ctggggctga aatggatgct tcaaacggtc
    30241 tgcgtgtgaa aaatccaact cacatcattc caaatatcaa caagaccgca ttgggacaaa
    30301 atgatagctt aaaaatattt ggagatgact acgatacacg tgacggttca tgtattcgag
    30361 attacattta tgtcttggac cttgcatagg ctcatgttaa aggaatgaac tacctatttc
    30421 aagaagacag ttcttctcaa atctttaact taggaactaa aaaaggctat actatcaaag
    30481 aaatctttaa aactgctgaa gaattactaa attaaaaaat cccacacgag attgttgctc
    30541 gacgtgctag tgacccagcc agtgtcctag cagatgcttc aaaagctaaa caatatcttg
    30601 attggacggc tagctactcc ctcgaagata ttattttatc agattaaggt aaaaacattt
    30661 tagaatagga taacaaagga gaaagtcttg ttgctctctc cttgttgtat ttattaaatt
    30721 gtcagaaatt ttattgacag attagaaaaa taatgttaca tttatatccg caggtatctt
    30781 tcgataccaa atctacatga aaggacgggg tatgaaactt tctcattatt taattggctt
    30841 acttctactc ctagtctttc tctctattag cattgggacc agtgattttt catggggaaa
    30901 gctatttgat ttcgaccagc agacctggct cctctttcaa gagtcccgtc tcccaagaac
    30961 tatcagtatt ctcctgactg cctctagtat gagtatggca ggccttctca tgcagactat
    31021 tacccaaaat cagtttgctg caccgagtac agttggaacg actgaagccg ccaaactggg
    31081 aatggtgctg agcctttttg tctttccatc ggctagtctg acccaaaaga tgctcttcgc
    31141 ttttgtttca tccatcctat tcaccctctt cttcctagcc tttatgacca tttttactgt
    31201 aaaggaaagg tggatgttgc ctctgattgg gatcatctat agcgggatta tcggctcagt
    31261 cacagaagtt atcgcctatc gtttcaatct ggttcagagt atgactgcct ggacccaggg
    31321 ctccttctcc atgattcaga cccatcagta tgagtggctc ttcttaggcc tcatcatcct
    31381 gataaccgtt tggaaattat cccaaacctt caccatcatg aatctaggga aagaaaccag
    31441 cgagagtttg gggatttcct actccctact tgaaaaactg gccctctttc tggtggcgct
    31501 aacgacaagc gtcaccatga ttaccgtggg tggcttacca tttctcggag ttatcgttcc
    31561 caatcttgtt cgcaagcgct atggagataa tctaagtcaa accaaactca tggtcgcact
    31621 ggttggtgcc aatctagttc tggcttgcga tatcctatcc cgagttctga ttaggcccta
    31681 tgagttgtct gtcagtctct tgctaggaat catcggtagt ctcgtcttta tcctacttct
    31741 ctggagaggg ggacgaaaag atgcagacta aaagcaaaca taccaagctc ttctggattc
    31801 tcattattct tgccatcgga gcttgtcttc tctacttttg gcccatcact cacttgtcag
    31861 cctttgcttg gaagttgcgt tcccaaaaga tcatcgttta tctcttggta gccatcgcga
    31921 ctgggatttc gaccattagt tttcaaaccc tgacggaaaa tcgcttcctg acgcctagta
    31981 ttttgggaat tgaatccttc tacgtcctac tacaaaccct actactggtt tttgaaagca
    32041 agtttcttca acttggcaaa tcccctatct tagaattcct agtcttactt cttgtccagt
    32101 ccctcttctt tctcgcctta caaggttact tgaagacact gatgaagcaa gacctggtct
    32161 tcatcctgct gatctgtcta gcgctcagaa gtctctttcg aaatatcagc accttccttc
    32221 aagtcctaat ggatccaaac gaatacgata aactgcaaaa tagtcttttt gcctcctttc
    32281 aacatcttaa cacttccatc ctagccatcg gttctctgat catcctcgct ttgacaatct
    32341 ttttctttcg aaaagcagtc gttctagatg tcttgcacct gcaaagagaa acggctcaga
    32401 tattgggact cgatgttgaa aaagaacaga aagagctcct ctggggaatc gtgcttttga
    32461 cctcaacggc cactgccttg gtaggaccta tggccttctt cggctttatg ctggccaacc
    32521 tcacctacct gattgtcaaa gactatcagc acaagttact ctttatagtg gccattctgg
    32581 ttggatttat tagcttaacc ttggggcaag ccttgattga acgagtcttt gcactggaaa
    32641 ttcgtatcag tatgatcatt gagagtgtgg gtggcttctt attctttatc ttactatata
    32701 ggagggctcg tcagtgaaac tggaaaacat tgacaaatcc attcaaaaac aggatatttt
    32761 gcaaggcatt tcgcttgaag tcagtcctca aaaactgaca gcctttattg gtccaaatgg
    32821 tgctggaaaa tcgactctcc tctccatcat gagcagacta accaagaaag atcagggagt
    32881 tctcagtatc aaaggacgtg aaatcgagag ctggaattcg caagaactgg cccaagaact
    32941 aaccatccta aaacagaaaa tcaattacca agccaaattg actgttgaag aactggtcag
    33001 ttttggacgt tttccctaca gccgaggtcg acttagatca gaagactggg aaaaaatccg
    33061 agaaactctg aactatttgg aactgaccaa cttaaaagac cgctacatca atagcctgtc
    33121 aggggggcaa ctccagcgcg tctttatcgc tatggtactg gcccaggata cggactttat
    33181 cttgctggac gaaccactca acaatctcga tatcaagcaa agcgtcagca tgatgcagat
    33241 tcttcgacga ctggtggagg aactcggcaa gaccattatc atcgtcctcc acgatatcaa
    33301 catggccagt cagtatgcag atgaaattgt cgccttcaag gacggccagg tctttagcaa
    33361 gggaagaacc gatcaaatca tgcaggctga cctgctcagt caactttatg agattcccat
    33421 cacgctagct gatatcaatg acaaaaagat ctgtatctat agctagtaac ataaaagctc
    33481 aagttagaga accttcagtc tcttagtcaa taagattaag agactcccta aatcgttatc
    33541 acattttaaa aaggagaaat tatgaaaaca tcccttaaac tttatttcac tgccctagtg
    33601 gccagcttct tgctcctact tggtgcatgt agtacaaact caagcactag tcagacagag
    33661 accagtagct ctgctccaac agaggtaacc attaaaagtt cactggacga ggtcaaactt
    33721 tccaaagttc ctgaaaagat tgtgaccttt gacctcggcg ctgcggatac tattcgcgct
    33781 ttaggatttg aaaaaaatat cgtcggaatg cctacaaaaa ctgttccgac ttatctaaaa
    33841 gacctagtgg gaactgtcaa aaatgttggt tctatgaaag aacctgattt agaagctatc
    33901 gccgcccttg agcctgattt gattatcgct tcaccacgta cacaaaaatt cgtagacaaa
    33961 ttcaaagaaa tcgccccaac cgttctcttc caagcaagca aggacgacta ctggacttct
    34021 accaaggcta atatcgaatc cttagcaagt gccttcggcg aaactggtac acagaaagcc
    34081 aaggaagaat tggccaagct agacaagagc atccaaaaag tcgctactaa aaatgaaagc
    34141 tctgacaaaa aggcccttgc gatcctcctt aatgaaggaa aaatggcagc ctttggtgcc
    34201 aaatctcgtt tctctttctt gtaccaaacc ttgaaattca aaccaactga tacaacattt
    34261 gaagactcac gccacggaca agaagtcagc tttgaaagtg tcaaagaaat caaccctgac
    34321 atcctctttg tcatcaaccg tacccttgcc atcggtgggg acaactctag caacgacggt
    34381 gtcctagaaa atgcccttat cgctgaaaca cctgctgcta aaaatggtaa gattatccaa
    34441 ctaacaccag acctctggta tctaagcgga ggcggacttg aatcaacaaa actcatgatt
    34501 gaagacatac aaaaagcttt gaaataagcc acttcaaact caatttaata ctcaatgaaa
    34561 atcaaagagc aaactaggaa actagccgca ggttgctcaa aacactgttt tgaggttgca
    34621 gatgaaagct gacgcggttt gaagggattt tcgaagagta taaaattttg acattctaaa
    34681 acgcatccta tcaagttcac tcaaacctga taggatgcgt ttttatcatt ttgaaatcta
    34741 tttaccccac ctcattcccc ttcttgattt cgtttaaggg aaaagtggtc tggaacgggg
    34801 tccttacctg ttttcgacca gggatgacaa cgtaaaatcc gagccaagcc catcaatacc
    34861 cccttaaaac catgtttttc aatagcctga atcatgtagt tggaacaagt cagctcaaag
    34921 cgacaagagg gtggaaagac tggtgagata aaacgttggt aaaagcgcac aggcgctatt
    34981 aaaattcgtt tcattatttc ttagttacag ccatggtgtg tagttggctg atttcttttt
    35041 tattaagacg acgggattct cctggacgga gtcctgtcaa gtctaggtgt ccgaaacgag
    35101 tccgagacaa cttatctact tggagaccaa cagcttcaaa catcttttta acctgatggt
    35161 tacgcccttc atggatggtc aactgcacca cagagcgatt tttgactggg tccactttga
    35221 gaatttcata aacagctggc ttggttttct taccatcaat ctcaagacca cgggtcaagg
    35281 ggcggagatt gtccttattg gccacacctt taacacgcgc gacataaacc ttgtcaatct
    35341 cattacgagg gtgaatcatc tcgtctgtaa agtccccatc attggtcaaa atcaagacac
    35401 ctgatgtatc ccagtccaaa cgacccacag ggtaaatacg ttctttgaca ttgggcaaga
    35461 ggtcgacaac cgtcttgcga cccttatcat ctgtcacact ggaaatcaca ccgcgtggtt
    35521 tgttaagcag atagtagacc ttttcttcgt tgtagatagg ttgaccttca acttcgacct
    35581 tgtcgcctga cttgatagtg gttgctagtt cacgcaccac ttggccgtta accgtcacca
    35641 agccttgctt aatcagctct tctgcttttc tcctactggc cacacctgcg tgggcaatat
    35701 acttattgat tctcatcttc ttctatcctt tcaccaaata attggctttc ttgggcttga
    35761 atctcaagct catcaatcac tggtaattct tctaaatggt ttatccccat gtaatctagg
    35821 aaataatccg tagtcacata gaggttgggg cgccccaata cttccttttt cccgtcttcc
    35881 tttatcaggt caaaagcctg caactttgcc aaggctccac tcgagttaac cccacggatg
    35941 gcatcaattt ctatccgcgt aatcggctgt ttgtaggcaa taatggacaa ggtctcaagg
    36001 gcagcccgag acaagctctg gttgataggc gccttagagt attccttcaa aatctctgca
    36061 aattgaggct tggtcaccaa tctataagca ccacttgtct caatcaaagc caaactggaa
    36121 tctgggtcct tttcatactt ctgggctaat tttcctaaac tttgctggat gcctgtcggt
    36181 ggcagagaga ggagttcagc taactggcgg acccgaatcc catcttcacc cgctacaaac
    36241 aagagcgctt ctatttttgc taaagtactc atctttcctc tctatcaagt ctagctttga
    36301 ggcacttgac tttcttcctt cttttccatg agatagatat ctccaaaact ctcctcttgc
    36361 acgaggatca gctcctgggt tttgattaac tctagggttg ccaaaaagag ggtgatgacc
    36421 tcttggacat tctgggcttc cttgaacaaa tcctgcaagc gcaattgatc tcgtccaatc
    36481 aaggactctt tcacgataat catcatgtcc tcaatcttat actcatcccg caagatcgtc
    36541 gtatgatttt gtgcaaactc ctcttttttc ttggctagga tatttgaaaa agccaaaaag
    36601 aggtcaatgg tcgtcttgtc atgcacaagc tccgcatctt cgtaaatcaa ctctgtcggc
    36661 gctttggaat aatactgggc ccgttcttgg tgcttggctt ccaagtgctc acccaagagc
    36721 ttgaacttgc gatattcttc gatttgagag aggaggtcct gctccaggtc atcccccaag
    36781 tctgtcactt ctgctacctt cggaaggagt ttacgactct taatcagcat gagctgacta
    36841 gccatgacca tgtactcacc cgtcacttcc agacgcatgg cctgcagggt tgagacatag
    36901 gctagatact gttcgatgac ttccgtaatg ggcacatcgt agatatccat ctggtactta
    36961 gaaaccagat gcaagagcaa gtccaggggt ccttcaaaat cttttaattt aatatccatt
    37021 atctatattt ttctaaggtc aggactgttt ttaatcctaa tttttttgca atttcgtaca
    37081 aatcgacctt gttttctatt tgtcttagaa taaactgttc acgtaagact tgagctgata
    37141 aggatggaaa acctttctcc ttgacaaaag attctaactg acgaaaggcc cactgacgag
    37201 aatagggttt ctctcctctt tcaaaaagat aggtctgccc catcaagggt tccaattctg
    37261 aaagcaaggc cgtgggaata gtgacaatcc tctgttggga agccttgctg attcgcaaca
    37321 cctgaaaatc cagattgatg tccgcaacct tgatggctaa aatctcactg ggcaaaagcc
    37381 ccatttctag gattaagagc gctagcaagc ggccctctgg atggtcgctt tcctgccaaa
    37441 aagagtctag gtatagaatc tctggctttt ccgtcttctt ttcagcttgt ttggctaatt
    37501 ccaagcggta aaagctgtcc acctctcctt tttgatagag aaagtataga aattggttac
    37561 aggccgaaat ctttcgcttc tgggcgctga tttttagatt ggctagctgg gcttggtaaa
    37621 tcttgagact ggtctcagaa atccgctcac ctaccatgtc taaaaattgc tccaaatcat
    37681 acttatagga ctgcttggaa ttgacagaca agccctgctt ttcctctaaa aaggctgaaa
    37741 tcctgtctct cattggcatc gaatctcata ttccttacta aagtcatgca agagggcatt
    37801 aacggccttg aggatggact tgcgcgtaat aatcccttgg aaaataccct ctgcatccac
    37861 aaccggtaag aaggactcat ctactagctt gtgcaagacc tccgtaatgg tgaaatcagg
    37921 cgaaacaacc gctacgtccg tttttgtcat atgaacgata tccgtatccg ccatgatttc
    37981 ttggctcaag tcatgctcca tctgataagc cataatatct ctgagtccaa tcgtcccaac
    38041 aaactgtttt tcatctgtca caacgggaac acgggtatag gtcatctgac tgagcaagag
    38101 ggtcgcatga tccgcattgt gggtatcaat caacacagct agatttttag caggggtcaa
    38161 aaaagtttcc tcctgcccca acaagaaagt ctcaaactcc ttggcaatca tcggctaaac
    38221 tccttggaca aacctggata cacctcgtga tctcgtgtca aaaagtccac tttgaagtaa
    38281 ctatcatcaa tctccacacg agcatagaga cattctctga tggtacctcg tggttgactg
    38341 atagaacctg gatttagaaa gaggatcttg ccttccaacc aagcacttgg cacatgcaag
    38401 tgaccataga ggcagatagc ggcctcttcc tcctgagccc agtagtccaa cttttgaaag
    38461 ttgaaattga tgtcaaacaa gtgaccatga gtttggataa tcttggtcga accaagctca
    38521 gtcaccagac gttctgggta gccggcgtag aagtccatgt tccctttaac aacgcggatg
    38581 ccctcccaaa gtggagaatc cggacgtagt tcagaatcgc cgttatgaaa aacggcatcg
    38641 actttgccca catagcgatc acggacttct tccacaatca agctatcgcc atgggaatcg
    38701 ctcattacaa tgatggtttg ctttgccatg atggaaatac ctccaaaagt ttcttaacgg
    38761 ctaaggcacg gtgagattga ctattttttt cttccagggt taattcagct gatgactcac
    38821 ctgtctctcc tacaaggaag aggggatcat agccaaagcc attttcaccc ttaggttcaa
    38881 agttaatata acctgaccag tctgcttcaa caactaaact ttccttattt gggctggcta
    38941 cgactagggt tgtgtggaac tgagccgagc ggtccttgag ttcaaagacc atggccaatt
    39001 cgtgcaagag tttggcatta ttttcacggt cagttgctcc cacacctgcg aaacgagctg
    39061 accagacgcc tggtaagcca ccaaggacat cgactttgag accagaatca tctgccaaaa
    39121 ccatcttgcc cgttaattga gaaatggttt ctgccttaag gcgggcattt tcttcaaagg
    39181 tcatacctgt ttctgctact tcaggcaggt cagggtagtc attaagattt tccacatcgt
    39241 agcctaactt atcaaagata gctcggaatt ccttggtttt accttcgtta cgagtcgcaa
    39301 tcaacaaggt ttctctgacc ttgcttgtct caaagaaatc atgaacattg accccttctt
    39361 taggcaattc tacatacaag agttgcccat tttcaaccaa gagcagggcc ccatgacgtt
    39421 gaataacttc caagtttcgt cccatttctt gattaagcat gtctaccaca aaggagaaca
    39481 aacggtaggt agaaccatag cgtaaaacag taactgaaag cgggaaacca tactcttcat
    39541 ctccaaatat tttggagaat tcaaacagag gaaaatctgt cttatagtcg ctcaaactgt
    39601 taacgccacc aaaaatacta taagacccaa cataccagtc ctggtcatcc ttatattcat
    39661 aaattttatt tgtcataatt ctacatgctc cacatgaatc tctttttcca gccattcttc
    39721 accaatttgt gcaaaacttt ggctactggc tgttgtgtaa aaacggtgat ggagtggtcc
    39781 agcatcgcga ccacgattga tttcaaaata attgagtaag actgagatat cccgtacgca
    39841 ctctgcccca ctatcgatga gctgaacctt tggccccatc acattttgga taatagggcg
    39901 aaggagtgga taatgagtac agcccaaaat caggctatcc acctttccaa ccaagggacg
    39961 cagggtttca tagaccacct tcttggtaac actggttgac agggcacctg actcaaccaa
    40021 gggagcaaac ttgggacagg ccaagctctc cacctgtaag tcgggatcca gatcatggat
    40081 tttctgacgg tatatgtctg attgtaccgt catgggcgtt ccaatcactc cgattttccc
    40141 accttgactg gacttgatgg ctgccgaagc tcctggcaaa attacaccca agacaggaat
    40201 atctagttga gccttgattt cttcccagac gaccgcagtc gcagtgttac aagcaatgac
    40261 aatcattttg acatccttgg tcaagagaaa gttgaccagc tgccaagtat attcacgaat
    40321 ttgctcagca ggacgggggc catagggcgc ccgcgccgaa tctccaatat agacgatttc
    40381 ttcatgggga agctggcgca tgagctcgcg cacaacggtc aagcccccga cacccgaatc
    40441 caaaaaacca attggtcgat tatccataca gtcttctttc tagtcttttt tctgattgat
    40501 cgttcattat gattccttgt actgaacgaa ctgaccgaca gcttgataga atgtcaacta
    40561 cctctctctt aatcataatc aaatatcaat agaatgcaag gaaaaagtct catccctaaa
    40621 ccataaccaa catagtgaga agtctggaac tttcatccca gacttttcct tatttatttt
    40681 ttctttttat tgttagcaag ggctgccttt tgttggcgga tgatttggtg gtaaacttgt
    40741 tgtaccttag cttcgcttgg tttttgacca tttgcactca aaagagtacg aactgcttca
    40801 gcattcaaac gtgggttgtc agcaaattct ttttcgattt gcttacgaac caagtacatt
    40861 cctccaagag ctcctcctag aaaagctagc acaatcaata caattgctaa aagtaaatcc
    40921 atcatttttc tcctgtttct ttttgaatcc ctttcattat accacaaact agccaccctg
    40981 accagtttgt tctacacttt caaggatgga atagacagca aaaagaaccc tgcttttctg
    41041 aaaaagaggt ctccttgcta agtttaattt acataaatag ccagtgtttg atagggtttt
    41101 agtagaattt tctcagacac ttctgcatct tcatagtttg aaatcaaaat ctgtccattt
    41161 tggtagactg ctggcaagtc gatttccact tctttagcat aaaagttatt gagcactagt
    41221 aacttttgat cctcaaactg gcgttcaaaa gcgtagactt gtttgctatc ttcaaaggct
    41281 ggtttgtaac ttccttctga aatgattggc atttccttac gcagtcgaat caagtcttga
    41341 tagaaggtaa aaatcggacc ctggatttca ttttctacat tgatgtcttt ataggattta
    41401 ccagctttca accaaggagt gcctgttgaa aatcctgcat tttccgaagc atcccactgc
    41461 atgggaatgc gtgaattatc acgcgactta gcttgaataa tctggaaggc ttcttgctga
    41521 ctctttcctt cttctaggag catctgatag gcattaagcg attcgacatc cacgtaatca
    41581 gccatagaat catagtctgg gtcaatcatc ccgatttcct cacccatgta gatataaggt
    41641 gtcccacgtg acaggtgaat gctggctgct agcatagtgg ctccttcctt gcggaagttt
    41701 tgaatatcga caaaacggtt caaggcacgt ggttgatcgt gattgttcca aaagagggca
    41761 ctccaaccgt ctttatcact catttcctta ccccaactat ggtaaagact cttcaactct
    41821 tcaaaatcaa agggagccaa ggtccacttt tgtccatcct tatagtccac cttgaggtga
    41881 tgaaaattaa aggtcatgga taattcctga cgatcaggcg acgaatagag gacacagttt
    41941 tccatggtgg tagaagacat ttccccaact gtcataaagc tatcgtcaga tccaaaagtg
    42001 gcttggttca tcatacgcaa atagttatga acgatgggtt tgtctgtata agctggcttc
    42061 ccttcatttt caggacagtc cactgaaacc tcgtccttac cgatcaaatt gatcacatca
    42121 aatcgaaaac ctttgacacc cttgtcgcgc cagaaattaa caaccttgaa aagctcctta
    42181 cggacattgg aattgcgcca gttaaggtca gcctgggtct catcaaatag gtgaagatag
    42241 tatttcccag tatccccgaa aggcgcccat gcagaaccac caaacttaga ctgccaatct
    42301 gttggttggt cttggatgaa gaaaaagtct tgataatact tatcaccagc tagggctttc
    42361 tgaaaccatt catgctctgt cgaacaatga ttaagtacca tgtccagcat aaagtcaatc
    42421 ttgtgctctt taccgacaca caccatttcc tcaaaatcag ccatatcacc aaaaagagga
    42481 tccactgcca tataatctga aatatcgtaa ccattatccc gttgagggct tggatagaat
    42541 ggattgagcc agaccatatc cacacctaat ttggctaaat agggaatttt ttcgataatc
    42601 ccacggaaat ccccaatacc gttttcagtg gtgtctttgt aagattttgg atagatttga
    42661 tagactactt ttcctttatc aagtgtcatc tgtttctcct tttctgataa aagggaggaa
    42721 gcagtcttcc gtccctattt gtgctatttc aattatactc aatgaaaatc aaagaacaaa
    42781 ctaggaagct agccacaggt tgctcaaaac actattttga ggttgcagat agagctgacg
    42841 tggtttgaag agattttcga agagtattag attcgtgtag cgaccatgag agatgctcca
    42901 gcttggatcg ttgtcggata agttccggga atagtcgctg tataagcatc ttggttggtg
    42961 atgataacag gagtttctgt caccagacct gcagccttaa tgacatccat atcaaaacga
    43021 atcagttgct gaccaactgt aacgtgatct ccttggacta caagactttc aaaacctttg
    43081 ccatcaagac ctactgtatc cataccgatg tggatgagca attcaactcc ctcgtcagag
    43141 acaatgccga tggcatgctt ggtagggaaa agaaccgtca ctgtcccatt aactggagag
    43201 gtcaactcac cttggcttgg ttcaatgacc agaccttgcc ccatgacacc tgatgcaaaa
    43261 ataggatccg tcgcttgact caattctttc acttggccag ttagtgggct gataatttct
    43321 accgaagtaa gttctactgg ttcatggttc acaaattctg cttcttcttg agcaacgaat
    43381 tctgcctgca agttcgtatc gccctctgtt tttgtaaaga gaccagcctt gcggaagaag
    43441 aaagtcaaga gcattggaac aacaatcgca actagcatag ttcctgcaaa tggcagcatg
    43501 tattgaggtt gaatagagag aatacctggc aaaccaccga taccaataga agccgcagtt
    43561 acattaaaag taacggataa catgcctgca agggctgaac cagtcatccc agcaacaaat
    43621 ggataaatat attttacgtt aaccccaaaa agagctggtt ctgtaacacc gagataggct
    43681 gaaatggttg caggaagtga aacctgagcc tcacgctcat catggcgatg catgaaataa
    43741 taggcaaaca cggctgagcc ttgagcaata ttagaaagag caatcattgg ccatagggca
    43801 gtgccaccag catccgcaat caattgtgta tcaatggcat tggtcatatg gtgcagacct
    43861 gtgatgacaa atggagcgta gagggcacca aaaattgcac cgaagagcca tttaactgga
    43921 ccagttaaac ctgccaagac aactgatgaa agtccttgtc caattgtcca accgattggt
    43981 cccaaaacag tatgagccaa aatcaaggct ggaatcaatg acaagaaagg tacaaaaatc
    44041 atagaaatga cttctgggat atgcttgcgc cagaagattt caagataaga cagactcaaa
    44101 cctgcaagca aggctgggat aacttgggct tggtaaccga tacgattaac agtaaaatag
    44161 ccaaaattcc aaacccagtt tgccgcgata tcagctgctg gcgttgaagc aaccgcatag
    44221 gcattgagca actgaggcga taccaaacag attccgagaa caattcccaa aatttggctg
    44281 gttcccatct tacgagaaac agaccaagta atccctactg gtaagaactg gaagatagct
    44341 tcaccaggca accaaaggaa gtgattaacc cctgcccaaa actgagagga ttctgtgatg
    44401 gtcttgccat ccaacatcga ccaatggaca ccttccaaga cattacggaa accgaggatc
    44461 aatcctccga ctatcaaggc tggaataatc ggagtaaaaa tctccgccag agtggtcata
    44521 acacattgga ccacgttttg attactctta gctgcagact tggctgcttc tttggaaaca
    44581 ccctcaatac ctgaaacggc tgtaaaatca ttataaaaga tgggcacgtc atttccaatg
    44641 attacctgaa attgacctgc atttgtaaaa gttcctttaa cagctggaat tgactcgata
    44701 gctttaacat tagccttctt atcatctcct aaaacaaagc gcatccgtgt cgcacagtga
    44761 gttacggcag tcacattttc tttgcctcca attgcctgaa gcagatcttt ggcttcttgt
    44821 tcaaattttc ccatttgatt ctccttatac ttgtcaaagc aagtttgatt tgataagtat
    44881 attgtaaacg ataccaaact tgtctgcaac ttgtttggta taaaaagggt gtttttttgt
    44941 aaattttcta gcacttgtat ggtaaaatag atacaggtgt tcattaaact agactgaaaa
    45001 cctatttaag caggcaaaat gaagaaatac caacaattat ttaagcaaat ccaagaaacc
    45061 attcaaaacg agacttacgc tgtcggagat ttccttccta gcgagcacga ccttatggag
    45121 caatatcaag tgagtcgtga taccgtccga aaggccctgt ctctcctcca agaggaagga
    45181 ttgatcaaaa agataagagg gcaaggttct caagtcgtca aagaagaaac cgtcaatttc
    45241 cctgtatcca acctaaccag ctaccaagaa ctagttaaag aacttggact gcgctctaaa
    45301 accaacgtgg tcagtctgga caagattatt attgataaaa aatcctcact gataaccggt
    45361 ttcccagagt ttcggatggt ttggaaggtg gtccgccagc gtgtggtgga tgatctggta
    45421 tccgttctgg atacagacta tctggatatg gaactcatcc caaatctcac tcgccaaatt
    45481 gctgagcagt ctatctattc ttatatagaa aatggcctca aactccttat tgattatgct
    45541 cagaaggaaa tcaccattga ccactcaagc gaccgagaca agattctcat ggacattggc
    45601 aaagaccctt atgtcgtttc gattaaatca aaagtctatc tccaagacgg gcgccaattt
    45661 cagtttaccg aaagtcgcca taagttagaa aaatttagat ttgtagattt tgcaaaacgc
    45721 aagaaataaa agactgagac accagatctc agcctttttc tttatcccaa aactaattca
    45781 tcactgacca gactttggcc actatttttc tggaagagat gcataaggtc ttcaactgtc
    45841 agatgctttt tctcttctcc cttgacatcc accactatct tgccttgata gagcataatg
    45901 agacgattgc cgtactcaat cgcatgattc atatcatggg taatcatcaa agtcgtccac
    45961 tgatggtact ccacaatctt ttgcgtcaag tccatcacca tttgactggt tttcgggtca
    46021 agtgccgcag tatgttcatc caaaagcaaa agtttgagtt tcaccagagc tgccatgact
    46081 agggtcaaag cctgtctttg tcctcctgag agatattgag tatctacttt taagcggttt
    46141 tcaagaccaa tattcaactc cttcaaggcc tcttggaact ggattctatc cttctccttc
    46201 acgccccaac caagccctct cttttgccca cgtctcaagg caatagccat attctcctca
    46261 atcgtcaaac gagaagctgt tcccatctta ggatcttgaa aaacacgggc aatatccttg
    46321 gctctctttc tgacactcag atgtttaatg gatttccctg ccagtagaag gtccccttcg
    46381 tccaccgata gattaccagc caagatattc atcaaagtgg attttcctgc tccatttcta
    46441 ccaatcacag agataaaatc tccctcttca acctctaaat ctaatccttt gaggacatgg
    46501 ttctcattga ccgtccctgc ttcaaatgtc ttgtgaatat tttcaagtgt taacaaactt
    46561 gccataactt tctcctccta ttcatttctc aatttcagac cacgaatctt taatctcttt
    46621 tgcaattctg gtgcaaataa aaccaaagcg agcaagattg cattaaagag acgtaccaag
    46681 ttttgatcta gatttggaat ttcatagata tttaaaataa tcaaacggta aacaatagca
    46741 ccgattccga tggataataa gtggcctcca atggtcaagt cgtgtatcaa gacttccgag
    46801 ctcaaaccaa caacaatggt tcctgtccca gaagtcacat ctgaaaatcc atcattttgg
    46861 gcaaacaagg aaccacatag ggcaatcaaa ccgtttgaaa tcatgtaacc aacaatcttc
    46921 atggtgttta cattgactcc attggcctca ctcatcggga tattgtcccc agtcgaacgc
    46981 aagaccaaac caatctctgt tttcatcaaa agggtcaaga ccaaacaagt aagagacaga
    47041 ctaaactaag tgagaaaaca gctccttcat ttgtcagacc caagctcgcc aactgtttaa
    47101 agacggttgc agaatctccc aaggaaagat tgggtacact gcccataatt ttaatattga
    47161 ttgaatagag ccctgtcaag gtcacaatcc ctgtcaagag agctgggatt ttcatattgg
    47221 tgtggagcat tcctgataca agacctgcta ccatacctgc caccaaagca agtaaagtcg
    47281 caagccaagg atttgcccct gcctgtatct gtgatacaac gactgctgcc cccagaggaa
    47341 aggctccctc agcagtcata tccgcaatat tcaaaatacg gaaagtcaag tagaccccaa
    47401 ttgccataat cgaccacaac aaaccttctg ataaactaga taacacaaaa ttcatcttaa
    47461 tcctccttat tccttgcctt ctaacttact aatatcaatt cccaatgcat ctgccatttc
    47521 ttgattggta tgcaattcca gtttttcagg caactcaact gctatatttt ctggtttctc
    47581 accttttaaa acacgaatca gcatgcgagc tgtctgccgt cccaattctt cataattggt
    47641 tccgtagtta tacaagccac caacagcaat catttctgtt gaaccaccga atactggaac
    47701 cttgtgttta atagaaacct gcttaactgt ttccatggtt gacgaaatga tattatcagt
    47761 tgggacaaaa accatatcca cctctgccat caaactctct gctgccgcct tgacgttatt
    47821 actgtccaag attgtttttt caacaacggt aaagcctttt tctttcagaa gtcgcttagc
    47881 ttcatccttt taaacaaccg aattgggctc gctctgagta tagaggattc caatcgtttt
    47941 agcctttggc aacactttct ttatcaaatt gatttgggtt gaaatggcat ctgatgactg
    48001 atcgcttgtc ccagttacat tgcccccagg atgttctctt gactcaacca acttggcact
    48061 gactgggtct gttacagctg aaaagataac tggtgtcgtt tgcgtcgtat tggccaagct
    48121 ctgagcagaa ggccttgcga tggctagaac gacatcgctg gattctgcta gttgctggga
    48181 aatcgttttt agatttcttt gttctccctg tgcattttgc aaatcaatct caatattttc
    48241 cccctcaaca tagccttgtt tggccagctc atccacaaat ccttctctag tagcgtctaa
    48301 ggactggtgg gtaataaact gcgaaatacc gatacgaaag acatcttttt cttatctgcc
    48361 ttcaactgat gcaaactgat cagagaagtc aagaggagcc ccactaccaa gagggggcta
    48421 gtgattttcg aacaactttc ataatgaaac tccttctcag aaaagataaa acaaaaaacc
    48481 gcctagatac tatctaaacg gatacttgaa ataacctaac aagttacaac ttagctagtc
    48541 catttagata ttcatctata tggaccacaa tcaaaaatct tagccacata gatacaaaca
    48601 aattgcttga actgtttgca tccatggtga catgtctaca aacactatct aaagtagcta
    48661 aagtaaaagt tttgattcat agttacggct tgtctcttat tcatttcttt ttctgctttc
    48721 ttttttgatg cttcctatcc taccaccttt ttcatcacct gtcaataata tttcagaatt
    48781 ttttaaaatt tttcgaaaat tctttcagga agctttcaaa gtttttcaaa tcagtccaac
    48841 aaaaaaagtt ttataattcc cataaaaatt gacatggaaa ttataaaacc attactagtt
    48901 tagtcctttt tgataacgtg ccaattcggt ttggttcgcc caaacatagt gacctggacg
    48961 gatttctacc atagacggct tatcagtctc atagtcgtgt tgacttgggt cgtaaacctt
    49021 caagaccttc ttacgttcca agattggatc tgggattggt accgctgaaa gcaaggcttg
    49081 agtatatggg tgaattggat tgttaaacaa ttcttctgtt tctgcaacct ctacaataac
    49141 acccttgtaa ataactgcga tacgatctga aataaagcga acaaccgaca agtcatgggc
    49201 gatgaagaga taggtcaggc cgagctcttt ttggaatttt ttgagcaagt tcaagacttg
    49261 ggcacgtaca gaaacgtcca aggctgaaat tggctcatct gcaataacaa agtctggttg
    49321 catgaccaag gcacgggcaa taccgatacg ttgacgttga ccgcctgaga attcatgagg
    49381 gtaacgagtc aagtgctcag caagaagacc tacttcacgg ataatatttt gaactttctc
    49441 tttacgttct tcttcatcct taaataaacg gtgattgtaa agaccttcag aaataatata
    49501 atcaacagtc gcacgttcat tcaaacttgc ggcagggtct tggaaaatca tctggattcg
    49561 acgaatcaat tccgcagctt gttcacgcga tttcttacca ttaatctttt gaccatcaaa
    49621 aatgatatct ccattacttg tatcatttag accgatgata gcacgaccaa tagttgtttt
    49681 cccactaccg gactcaccta caagcgagaa agtttctccc ttgttgataa agaagttagc
    49741 atttttaacc gcgacaaact tcttacttcc ttcaccgaag gaaatttcta aatctttgat
    49801 ttctactaat ttttcagaca tttccttcct cctagtcagc cagatgggca aatcccattt
    49861 tttcacggat cttatcatgg agatttgcaa tcacagctgg tttttctact tttggagcat
    49921 cctcatgaag aagccaagtt ttagcccaat gtgtctctga tactgagaat tgaggagctt
    49981 tttgttcgaa gtcaatctgc attgcgtagt cagaacgcaa ggcaaaagca tcccctttca
    50041 ggtcagtata aagtgacgga ggtgttcctg ggattgagta aagatcccct ttatcatcag
    50101 caagctgagg caagctagac aagagactcc atgtatatgg atggcgaggg tcatagaaga
    50161 cttcctcaac cgttccatac tcaacgattt ctcctgcata cataaccgct accttatccg
    50221 caatacttgc caccacacca aggtcgtggg taataaagat tgttgtgaaa tgatactcgt
    50281 tttgtaaaga ttttagcaaa tcaataatct gagcttgaat agttacatcc aaggcagttg
    50341 ttggctcatc acagatcaag acatcaggtc ggcaggcaag ggcaatcgca ataacgatac
    50401 gttgacgcat tcctccagaa tattggaatg ggtattcatt aaaacgtcta tctgcgtctg
    50461 gaatgccaac cttattcatg tagtcaatgg ccaattcttt cgcttcttta gctgtttttc
    50521 cttggtgttt tacaataact tctgtaatct gactaccaat tgttttaatg gggtccaaac
    50581 tagtcattgg gtcctggaag atagtcgcaa tcttagcacc acgaatttgt tcccaatcct
    50641 tgtgagaaga taaagctgtc aagtcctgac cacggtagtc aatactacct tgggcaatac
    50701 gaccattttc ttcgagcata cctgtgaagg tctttgtcaa aacagattta cctgatcctg
    50761 actcacctac caaggctaat acttctcctt caactagttc aagggaaacg ccgcgaatgg
    50821 ctgtcaatac tttgtcacga acgtcaaatt ccacgacaat atcgcgagca gtcaaaatta
    50881 catttttttc ttttgtcatt tctactccta tctatgtgta cgtggatcac tagcatccgc
    50941 taagttttga ccaactacga aaagggacaa ggataccaag acaagggttg tcaatggaat
    51001 ccagaacaag taagcattgg ttgttacgtt ttgtgaataa tccgaaatca aacgacccaa
    51061 acttggcact gtaatcggta atccaagacc gaagaaagac aagaaggctt cgtatgagat
    51121 aaagcttgga agcatttgag tcatggttgt cacaataaca gataccaatt gaggcatgat
    51181 atttttggca acaatcttca aggttggtgt tcccaaagta cgtgacgcca agttgtattc
    51241 caagtcacga tagcgcaaga tttgcacacg gatcatgaag gcaataccaa tccatgttgt
    51301 tacgctcatg gcaaaaatca gattccagaa tccagctccg attgagtaag tcaagacaat
    51361 aacaatcaaa agaggtggga tgtttgagat gacgttgtaa acttccatca tgacacggtc
    51421 aactgatttt gaaatacccc aaataccacc gacaaaaaca ccgataacca agttaatcac
    51481 tgtcgcaatc acagaaatga ggatggagtt acgagctccg aaccagacac cgtcaaagag
    51541 cgatttaccg ttactgtcag taccgaacca atgctccgca tttggcttga tataacgaac
    51601 actaaagtcg tttaccttgc tgacatcatt gaaatcaaac ttagaaaaca ttgggtagat
    51661 gaaacttatc aaaatgatgg ctaccaagat tcccaacatg actacagttg attttttctt
    51721 cataaattgt ttaaacactg atttccagta agaatatgct ggcgcatcaa tagtttcaga
    51781 ggcaaaatcg tcacgtttta caaactgaaa tttttcttta tcgattgtag acattatttg
    51841 cctcctttct cagtcaattt aatacgtggg tcaataatag tcatccaaat atctcccaaa
    51901 agacgtgaga agatagaaat acatgtaaag atgaagacaa gaccaacgac catagagtta
    51961 ttagatgctt ttacagagtc aatcaacatt ttacccatac ctgggaaggc gaagactgtt
    52021 tcagtaaggg ttgcaccacc gataacccca ataatggcac caggaattcc tgaaaccagc
    52081 ggaaccatgg catttttaaa gatgtgtttg tttgaaattt ctttttcaga caaacctttt
    52141 gcacgagcga aacgaacaaa gtcttgagat tgcaagtcaa tcatgtaacg acgaatccaa
    52201 atggctgtaa caggagcacc caacaaacca aggatgactg ctggtaaaac gtaggaacgc
    52261 caatctccag ctcccaagat agggaatgaa tctggaaggg caatagatga tccaatcaat
    52321 cgaacgatgt aaaccaaggc aatcgttgga agagcaagca agaaggtcaa agtccctgtt
    52381 gagaggctat caatccaagt gttcttgaaa cgagccatgg ctgaaccaag tggcacggca
    52441 agagcatagg caagaaccaa accaatcaaa ccagtaatag cagagctgac aatcatagat
    52501 ggatattggt aattactttc agtcgctgta taaggatcat ctttcccata gctagctact
    52561 tcacgagagt cagcctgact aggtgacttg taggttcttg agtaaatatt tacagaagac
    52621 gttttcttac ctgttgggaa ctgaacttgg gcagttttgg tttgtccttg accttgagta
    52681 ataacctgaa gaactggtgt attagcatag gttgggtaag agtcacctaa attcaagttc
    52741 acaaagtttt gatgaacaaa tgggaactga ctgttaaagt acaagagata tttatgttta
    52801 gttcctgaac cgaccaatga ccatccgata gctggatcat tttcaaaacg aaggtagcgt
    52861 ttcaagtctg gattttcagg gtcttggatt ttatttgtat ggtcaatgtc aatcaagtta
    52921 gcatagaagt gaaaaacacg ttcaaaaatt ggaatttcac gagtagcata gaattgacca
    52981 ctttcagtaa attctcccaa agtccaacca tgacctaatt gattgatgta cttttcataa
    53041 atagctttat tggtcgcatt tgcttctact gttacagaag aatccatgct acttgccttt
    53101 tcttgcaact ctttagtatc gtaatactca atgtagccca tacgctcaaa cacagtattt
    53161 tcatagttat cacgtttatc agccgttgtc gcaattttat tatagttagg atcctgcttg
    53221 aaaatcaatt ttcgaggaac caaggtatag ataatcgtgt aggtcaaagt cgttactaag
    53281 aaaatcgaaa ccaatgaccg caaaacacgc ataaaaatat attttttcat attatttcct
    53341 ttaaaaatcc caaaagaacc ttctcctcat ggagagaaag ttctattaga aattatttac
    53401 ttcacatgac ttgccaattc tttttgagct ttctcatttg attcagcttt ttctttcaac
    53461 catttttcac gagctttttc atactcttcc ttagtcacca ctttatcttg taatttcaaa
    53521 tatttgaagt aaacatctga ccccttagag cctgtttgcg cagaagctcc agtaaatgga
    53581 acaattcgtg aaagcactgg tgctgcacca gaagaagcca tagcaggaat aaagagtgaa
    53641 ctatctgtca accatgcttg agccgctgca tatttttcat aacggacatt caagtcgctt
    53701 gtctctctgg cagcttcatc aactaattta tcgtattctt tcaaaccaac ttgaactact
    53761 gaagggctat ttggattatc aaatcctaaa tatgtttttg tagtttcact gctagttgtt
    53821 tttaaaatat ccaggtaagt agatgggtct tgatagtctg gaccccatga aactcctcct
    53881 gatacatccc aatcctcaga tgaagcattg gcagcatagt aagtaatatt aaggaattca
    53941 tcacttgtca tttgttgaat atcaacaacg acattttcaa caccaagaac tgtttctaca
    54001 gattgtttaa aggactgaat acgagatatg tagttttttg atgcttggtc tactggaacg
    54061 tccagatgaa taggaaactg aacgccgtct gcttctaaag ctttcttagc tttcgcaaac
    54121 tctgccttgg ccttgtcaac attgaataaa ccatcctgcc catcagctaa attcacacct
    54181 ttccactcat caccataagc aggaagttga gcagcgacta aatcaccaaa ggtcttctca
    54241 ccagctgaaa caaagtctgg ttttacaaat aaattacgaa ctgctaaagc tgctccatct
    54301 ttaccattga tttgagctga gtaagctgag cgatcaagag caaaattcaa ggcttgacgg
    54361 aaatctttgt taagcaatgc cttcttagta gctactttct ctgaatctgt agttttagaa
    54421 gtatagttgt aactttggcg atcaatattc acacccagac cagcaatccc agagcctgat
    54481 tgtgtgtaat agatattgtc cttgtattct tctgcaacct tagaatagtt ggagctggta
    54541 gggtaaagac gggcataact ataagctcca ctagtgaagt tacgctctag cgactcctga
    54601 tctgatccat catagtaagc tagattgata gtatctaggt ggacattttc tttatcccaa
    54661 tattgctcat tttttacaaa ctctacagaa gattttgcag tcaacccttt caacaagaat
    54721 ggaccattat aaagcaagga tgtcggatct gttggtttag caaaatcgct tccttttgat
    54781 gtttcgaatt cttcattcag aggccagaaa atagaatagg tcaacttaga gttccagaac
    54841 ggttcaggct ggttcaaagt gtattgtaac gtataatcat caaccgcctt gacaccaact
    54901 gttgaaaaat ctgttgaagt tcctgataga taatctgcca agcctttaac cgaattttca
    54961 gctaaataca tagcttctga ttttttatct gctgcgtgtt ttaaaccgtt cacgaaatct
    55021 ttagccgtca cctctgcata ttcttctcca tcagaggtaa accatttaac ccctttacga
    55081 atcttataag tgtaggtcaa accatcctta gagacttccc aatcctctgc aactgcagga
    55141 gcaagattac cgtaattatc gttagtgaat aaaccatcaa tcccatttga agtcactact
    55201 gttgtactat ttttacttga aatcaggtag tccaaggttt ctgggtctgc tgtataaaca
    55261 tagccataag ctttaggggc tgatgaatca gatgattttg aagaactgca tgctgcaagt
    55321 acacctgctg ctaataaaac aagacctgct gtagcaaata cacgattttt tttcattttc
    55381 tactcctctg tttatgtgaa ttatagattg acaaccatta tatcacatta tccattaaaa
    55441 atcaaacaaa ttttcagaat atttaggctt gttggcacaa atttttcatt ttttttgaat
    55501 atatgattca aattgtcgtt cgaagtgtca aagactacag tgaaaatagg aaatttgacg
    55561 cagaaacttt ggagtttagg aagacataca gtaaaatgaa atacggacgg aacaatgtga
    55621 ttttggaatt caaattaaat tataacaata ttgtagaagt atcattctag tattcaagat
    55681 tcagtttact atgtcttttc acaccaacct tattccgaat tcaattactt ttgtgattta
    55741 catatataga ttaagactat cttttatact ttaaaatttc tcgctacctt atccactata
    55801 tgctcctcgc tatcacgttt ctattcatag cctacgattt cactattgct ttctctgaca
    55861 attcttattt cctgcgtcag acttaaaacg atctatcccc agaccatttt aatccgctac
    55921 ctcacgatag tcaggcttgg ggagcgctat tgtattcacc ggtagtggat ccctacagag
    55981 gacttacacc tcagatgcac gacatgccca tcgtataaaa aaatctccta cccaaggtag
    56041 aagatttcaa acttataaaa cttaatccgt catgtccgat accaacattc gatgctccaa
    56101 tggaatactg cacataacta gcaagaaaat aaagcctgac tgaatccaga agagagccaa
    56161 gtcaaaaatt ccgtgcacag caaccactgt aaggaaagat agataaaggc cgataatcgg
    56221 acgtttcccc gactcctgac tcatatccat catcaagcga acaggagcaa cagaagacaa
    56281 aactaataaa atggtaccca caattccgta actcagaatc gtatcaatat aaagactgtg
    56341 ggcatgttca tgataaggag catgtatccg aggataagag tgcatatagg tcaatggccc
    56401 ttcaccccaa aaaggatttt gcttaaacaa ggccatccca gcatcccaga tagaaatgcg
    56461 ttcttccata gaagagtcta aagtacccat tcgaactccc aaatcactag aaaagaggaa
    56521 actcaaacca atcgcgaaga ccccaatact aagccaaaag gccttccagt ttttaatagt
    56581 cgtaaagaga tagataattg ctccagcgat aatagcagga aaggcagttc gattttgagt
    56641 aaagttcaaa ccaaagagat taacaaagcc tgcaatcaca cagaatactt tcaaccaatt
    56701 caacttggtc gttgtaaaca gatagaaagc aatcataata cagaaacaac aaataattcc
    56761 ataataatta ggattaaaga aggtcacttc tgcccggttc tgatgccaca cctgcatatt
    56821 gggtgaaaga aaagcatagt taaatttctt cacaatttgg aaatgttcta aactggcaaa
    56881 agcagctgac aagacactac caaacaagac gaactgcaaa atcaatcgaa agaatttatg
    56941 ggataaaatc gactgatagt gcaaaaagaa aatagtaaat agaaacattc ctactgaagc
    57001 cacaagaccc atccaatttt gtgcaagaat ggatataaca gtactatagc taagaaaaag
    57061 aagcagcatc ggatgctccc ccattttctg aagaatactt ttcatgtctc ctgtaaaaat
    57121 caaactgata atatataaac agagtacaac tacaaaaaga taaaagggta aaaagatact
    57181 taggataatt cccaataaaa tcagctcttt actagacaac cccttcagct tttcaataaa
    57241 gcctattgat ttcaaaatga atcctttctc tccaaatcag ctgattcaga taatagtaag
    57301 ctatcctata ttgtaccact tttttagcaa tttgaaaaca aaggaaacgt tttccaaaat
    57361 aaaaacccta ttttatccac catatcaagg cttcaaaatg atacttcaac tccattctca
    57421 attacccgat aagtctgatt ttgcaaatca atttctacta ctgctgttac ggacttatct
    57481 ttattttgac gtttgattac aatgctgtga gctgttggtg tctctatctc agtagtccct
    57541 tctagatcaa aggcttctga acggttacgg aaagaaaata gattgagaag ggccttcaca
    57601 acaggtcgtt gcacttcttt tgctatttcc tcgttgctat agtaatgacg attaatattt
    57661 cgaccttctt tagtttcttc taataatttc aagtcattct tgcctgctaa tagacccaca
    57721 tagtaaacct gaggaatacc tggggcaaaa gcttgaatta gacgagcgag aaaatacttg
    57781 acatcatcat ctccaagcgc tgaatagtag gttgaattga tttggtagat atctaagttg
    57841 ttatactcgg cactagagta cttacgtttg acattggctc caaccttata gagttcattt
    57901 gaagcatagt caatctcctc atcggtcagg atatccttga catctactac tccaatccca
    57961 tcatgggtat ctagcgtcgt aaattgcttc atcgggctca tctttaacca cttagccaaa
    58021 cgctctgttc tggaactgta aagagtataa agtgtcacca ttggaagagc aaaatcataa
    58081 acatagtaat catggtctgc tattttaaac tgaatcgaat agtgttcatg aatctcaggt
    58141 aaaagctctg tcccatactc agcagcgata tctcgaactt tgtccaataa atcccaaata
    58201 tctggttcca caaagaaatc attagtatcc aatttcttca ctgcataagc aaaggcatct
    58261 agacgaatca aatcacaccc attacttgcc aagtgctgaa tggtcttacg gataaattcc
    58321 atagttactt ctttggtcac atcaagatca atctgctctt caccaaaggt attccacaaa
    58381 tgttccactg aaccatcttc aaacacaatc tcttgctttg gtgcacgatc cttacgcttg
    58441 taaattaaat ctacatcaga ctgtgtcgga cggttttctg gccaaaactt atcccagttt
    58501 aaaaagagag ctttaaattc actggcttca tgtttttctt gatagtcctt ataatacttg
    58561 gattgacgag aaatatgatt aatcataaaa tcaaacataa gataatatct ctcacctaaa
    58621 cgcttaacat cctcccaatc accaaaagct gagtccactt cgtcgtagtc aactggcgca
    58681 aatccacgat cacctgttga tgggaaaaat ggtaaaaggt gaactcctcc aatagcatct
    58741 cgaaaatgct cttccaaatt atcatataag tctttaagat tatttccaag gctatcagaa
    58801 taggtaatca acatggtttt attttgaatt ggcatcatta ctctcctttt tctaattgaa
    58861 gccaagtctc atatgatctg gcttcataaa taaaattcat tttaaatctc tatttatcat
    58921 caaactcgta ctaatataga ctgtgataaa caaagtacta ctttcttgtt ttctacatag
    58981 aattatcaac aagctgaact cttcctctgt gtcaaagact atagattcca tgagctcttc
    59041 ttatactatt cgaaaatctc ttcaaaccac gtcagcgttg ccttaccgta ggtatggtta
    59101 ctgacttcgt cagtttcatc cacaacctca aaacagtgtt ttgagcaacc tgcggctagc
    59161 ttcctagttt gctctttgat tttcattgag tattacttca ctgccccgtt gctcattcct
    59221 gaaatgatat ggcgttggaa gaagagatag acaatggtga tactgataat gccgaccacg
    59281 taagaggcaa agcttggtcc gtagtcgttg aaatattggc ctgcgtagtt gtattggaac
    59341 aaaggcagag tccacatttt ggaatcccgg ttcaagacaa ggagtggcaa catgaagtca
    59401 ttccagaacc aaagggcatt gatgatcatg gttgtcgcat gcatcggttt catcattggg
    59461 aagatgatgc ggaaataggt tgtaaattga ttagccccat cgatctctgc tgcttcatcc
    59521 agactttctg gaatcgagat tttgatatag ccaacataga gaaagagggt ctgtggaatc
    59581 gcataggtca agtagagcaa gatcaaacca aaggtattag ccaaaccgag tttactcatc
    59641 ataaccgtaa tcggaatcat gatgacttgg aaaggtacga agattccgag gattaagagg
    59701 gtatacatga tggtaaaggc ttttctttta ctcatattgc gagcgatgga gtaggctgcc
    59761 atagggataa agatcattac tgcaagtaaa gacaagacag tgatgacgac agagttccaa
    59821 taatagcctc caatcccatc agctaagaga cggctaaagt tgtcccatgt gaagttggtt
    59881 ggaaaaccaa agaaattatc tacaatatcc ttagtgggtt tgaaggaact aaagagggta
    59941 gcaaggagcg gcactaaaat cagaaccgtt cctagaatca atagaatgta tttgccaatc
    60001 agggcttttc tttcatcttg tttcatcatg cttctcctct taaatttcaa atttcttaga
    60061 tactctcaat tggatgatcg aaatcactac aattaagaag aacaagatta cggcaatggc
    60121 attggcataa ccgaattggt tgtttttaaa ggcatagtta taaaccaaga gcccaagtga
    60181 ggttgtggca ttgtttggac caccaccggt catggcaaag acttggtcaa aggcagtcag
    60241 cccacctttt agggctagga taaagactat agagacactt ggtagcaagt aaggcaattc
    60301 aatgttccag aaaacttgct tgctagtcgc accatcaatc cttgctgcct ctgtaatctc
    60361 agttggaata gattgcaaac cagctaggaa gatgatgatg ggcatagcca ccccttgcca
    60421 aagaaggaca aagacagccg caaagattgc tccccactta gtccctaaaa gactggtttg
    60481 gagaaattca atatgaaggg cattcccaat cgctggaaga ccgtagttga agacttgctt
    60541 gaagatcaaa gccactgtca aaccagataa aacagctggg aagaagaacc aagcacggaa
    60601 gaaggtttgg cctttgattt tagaattcaa gacacgcgca atgaagatcc cgagtgcaat
    60661 ctcaccaacc accatggcaa tcgtaatgat tgcggtaaag ccaatcgcat tcatgaattt
    60721 tggatccatg aagaggagct taaagttgtt taagccgaca aatttgtagt tataagtcaa
    60781 tcctgtccag ttggtaaaac tgtaaaaggc tccttgaaac atcggcacat agaagaaaat
    60841 tgcttgtaac aagaggggga tgaccacaaa agcccatgcc caatattttt gtaatacttt
    60901 tttcatagtc tctctactcc taatccacat ccgctttcat cgggttaaag aaggcattca
    60961 aatcattgac catgccttgt ttatcaccgg tcaagacata gttcatggtc aaggtatgga
    61021 agtctgcttc actggtccag tattgttgca accagaccaa gtgacgatcc gtaaaggcat
    61081 attcggtcat accagcaagc ggtgaatctt ctcctgcttg tttgacccct tcgatcgctg
    61141 ttggagatcc gtccacatcg tagtattttt gcatgacttc tggacgggtc atatattcca
    61201 caaaggcatt ggcttctttt ggatgtttgg tggtggctga gatagaccat gccaagtctc
    61261 ccgcaccaac ggttaagctt tgtccttttt cttttcctgg aatcatgaag gtcccaatct
    61321 taaagttcgg tttttgttca ttaatcgctg tgatcgccca agacccattt ggtgtcatga
    61381 ggacatcccc acgtgcgaag gctccgataa catcggtata gccagcacct tcccagttct
    61441 tttgcttaga gccattgatg cgaaggatgt ccatgacctt gatgtcatct ttcataatcg
    61501 gatccgacaa tttaatggca tttggttgag aataacgaag gtattgattt gcttcttttc
    61561 ctccacctgt tgctgtcgca aaggctaatt gattgtaacc attgagtgtc caagcatctg
    61621 cacctgcaat tccaaatggt gtttgtcctt tagcaacgat atctttgact aactgttcaa
    61681 attcatccca ggtttcagga actttcaagc ccagttcttc gaatttatct ttgttgtagt
    61741 aaattccata agcattagct gtaaaaggaa cgttgtaaac tttttcgttt acagcatatt
    61801 tttcagcgta gccatttttc acgcgtttca ggtagtcttt gttactcaaa tcttcaaaga
    61861 cacctgcttt tgcccattct tgcagttcga tggactgtgg gtaaatattg accacatcag
    61921 gcacatctcc tgcgagaacg cgtgtcttca atacttcacc agcatttggt acattgacga
    61981 ctttgacctt tatcttaggg ttttccttct caaaatcacg agtgatttct tccaaggttt
    62041 tggtcatttc ttttttctgg ttgaaatact cgatggtcac tgtgtcatcc gcagatttac
    62101 catagttgga gcaagcaccg agcccaaaca aagctaaacc tgtagttgca agaagtccga
    62161 tttttttata ccattccatt agaaagcctc ctttataaat ttatacaccc ttatcaaact
    62221 gcacctcaaa agttagatag aataaatcta acttttgagg tcagtacata tcatagtttt
    62281 ctaaaaatat actgtctact caaaaaatct ccttgggata agataacagt taagcccgca
    62341 tacattagtt ctgcacctga gtaaacttcg ccattttcct gtaatttata tagtccctct
    62401 tcatccaaat cttttaattt taaagttgtt tccatggtct ctacaacaga taaaacgcga
    62461 acgtaggtta caatcgtttg atttccgtaa ttaaattgta cagctgcttc attggatgca
    62521 gtatcaggat taattagtct atactgctgt cctaactgaa ctactggtcg taattcttta
    62581 tacaagttca cctgattagc aatcgtagct ttctcttcat ctgataaatt tgtcaaatca
    62641 agttcatagc ccaaatttcc catcattgct acaagaccac gtgtttctaa tggtgtcatt
    62701 cgtcccatct gatgatttgg tactgctgac acatgagccc ccatagaaat ggttggatag
    62761 agataggatg aaccgtattg aattggtaaa cgtgcaatgg catcagtatt atcactagcc
    62821 cagacttgtg ggaaatagcg catcatacca agatcatttc gtccaccacc accagagcag
    62881 gactcaaaga gaatatggct gtgcttctct gtcagataag aaacgagttc gtaaagcccc
    62941 agcatgtact gatgagattg catctgtgtc tctagataag ttaatccatt ccctagctta
    63001 gtgatattgc ggttcatatc ccatttaatg taatcaatat catgataaga taggagttga
    63061 tctaagacac ttttcaagta ttctactacc tgaggattgg caagattaag tactaattga
    63121 ttccgagaat aagtatgctc atagtcagga acctgaatag cccagtcagg atgtcgacga
    63181 tacaaatcac tatctacaga aatcatttcg ggttctaacc aaagtccaaa ctgcaaacct
    63241 ctttcatgga tagctgaaat cagactttct agacttccac ccagtttttc ctcattaaca
    63301 acccaatcac ctaaagcacg attatcatca aaacgattgc caaaccaacc atcatctaat
    63361 acaaaaagtt caatgccaac tttcttagct tcatctgcta actctaacag tttttctctc
    63421 tgaaagtcaa agtaagtagc ttcccagtta ttgattagaa ttggacgttc ttttttagaa
    63481 aattcactta gcataatgtg cttcagtaca aaattctgac tttcatgact aataccagtt
    63541 aatccctgat ctgaatgagt cactaaagct accggtgttt caaagtattc ctcaggggct
    63601 aacttccaag aaaagttttc tggattaatg ccaatagcca cccgaacttc attcaattga
    63661 tttttttgaa caaaagcttc aaagttgcca ctatacatta gttgaatagc aaacacattc
    63721 ccagcatcct ctgtgactcc ttgttcgcat agtagaagag ctggtgtttg agtatgacca
    63781 gaagcacctc ggtttgagct aatcgaaaag attccttgtt ctacctgttg acgtctaaca
    63841 gtcttttcac gagcataagc accctgcaga gttactattt cgtaatctgc agctggaaaa
    63901 tcagccataa aagaaaaatc tttatggatg acaacttcct gattactatt attatctaat
    63961 ttactgtagc tagcaatagt cgcatcatta ttaaaagtag tataatacaa agtcagacta
    64021 agttgagcct tagaatcttc taacattaag acaagagtct ctgtatcgtc catgctatgt
    64081 ggagaaggta agccctgtgg accattctga ccttttaaaa tctttgcttc tacaaatcga
    64141 aagtctgtta cttcagttac actatgctga acctgtatgg ttggtttcct aaaatctcct
    64201 aagccatgtt gtccaaaaat ctgtcgctga gtatctaaac taaaggttcg attagtagcc
    64261 gttggatttc ctgaaaaggc atggtctcgt tcataaacac tattggaacc tttatagttc
    64321 ttaatagtct ttcctaaatg tttcaaaagt aagtagccat tttgattttc aataatcaaa
    64381 cttagatttt tactctcaac ataaaataga ttattctcta tcctaactcc catttacttc
    64441 acctcatcac tttattgatt atattttatc acctgaaatc gctttccaaa atagaaaaat
    64501 gtctcaagaa tatggtaaaa tgttaggtgg gaggtagcac atgttagttt tttcagaata
    64561 ccagactgga acaatcgacc ttgccctaag cttttatgga tatgaggaat gcacacctaa
    64621 ttactctttt ggtccagcca ttcgtgatac atacgttcta cattacatta ctaaaggaca
    64681 aggaaaattt tattacaagg gtaaaattgt tgatttaaaa gaaggagatt tctttctatt
    64741 aaaaccagag gaactaacct tttatcaagc agatagtaaa gaaccttggg cctactactg
    64801 gttaggaatc actggaggga aatcccctga ttattttgct ctttctcaaa tttctgatca
    64861 atcctatctc atccaatctg aaacttgtca tacccagact actgcaaaac tcatctcaga
    64921 cattgtccgc ttcgctcaga ttacaaaatc aagtgaatta gctcaactcc atatcatggg
    64981 acaacttcat gaactgatgt ttcatctggg aactattgct cccaatcaga aaaaaaagaa
    65041 tatttcatca acccaccaac tctatcttga atgcaaacga ttaattgata gccactatcc
    65101 tcaatcactt acaattcaag atttagcaaa agaactatcc gttcacagaa gctacttatc
    65161 aagcgtattc aaagaattta ataccttatc acccaaagaa tacctactct acgttcgaat
    65221 gcaccgagct agacaacttc tcgaaaatac ccaagagtcc atcaaggtaa ttgcatactc
    65281 ggtaggtttt tcagatccac tccatttttc gaaagcttat aaacaatact ttaatcagac
    65341 tccaagtcat acaagaaaag aatactctca ataccaacta gtaagaaagg caacattatg
    65401 aaatcctacc aagctgtcta ccaaatccta tctaaagaaa ccgactatat cagcggagaa
    65461 aaaatcgcag aaaaactatc cctaagccga acagcaattt ggaaagccat caagcgacta
    65521 gaacaagaag gcattgaaat tgatagtatc aaaaatagag gatataaact gatgaatggt
    65581 gaccttattc ttccagagat tctagaagaa aatcttccaa ttaaagtcag ctttaaaccc
    65641 gaaacaaaat caacacaact agatgcaaaa gaagcaattg atttaggcca tgaagcaaat
    65701 accctctatc tagcttccta tcaaacagca ggccgaggcc gttttcaacg ttccttctac
    65761 tcaccacaag gtggtattta tatgacactc catcttaaac caaatctccc ctatgacaaa
    65821 ttaccatcct acacactact tgtagctgga gctgtctaca aagccattaa gaacctaact
    65881 ttaatagatg tcgacataaa atgggtcaat gatatctatc taaacaatca taaaattgga
    65941 ggaatcctta ctgaagcaat gacctctgta gaaactggct tagtcacaga tatcattatt
    66001 ggagtaggta tcaatttcac tattaaagac ttccctcagg aattaaaaga aaaagctgcc
    66061 agcttattta aagctacagc tcctataaca aggaatgaat tgatcataga aatctggcgt
    66121 gctttcttcg aaacaccagc agaagagcta ttatacctat acaaaaaaca gtcattcatt
    66181 ctaggaaaag aagtcacttt cacactagag caaaaagact acaagggact tgctaaagac
    66241 atctcagaaa atggaaaact tttagttcaa tgtgataacg gaaaagaaat ctggctaaat
    66301 agtggcgaaa tttctctcaa tagttggaag taaaataaca caattataat ataaacgata
    66361 taaaaataac ttcagattag taattcaatt aagttttacg gatctgaagt tttattggct
    66421 ctaaaaataa aaaagagagt tacaaactct cattaaaacg gagaataagg gattcgaacc
    66481 cttgcgccag ttacccgacc taacgattta gcaaaccgtc ctcttcagcc tcttgagtaa
    66541 ttctccaatt
//
LOCUS       DAVLYV010000003        59365 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLYV010000003 DAVLYV010000000
VERSION     DAVLYV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 59365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.003"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(238..318)
                     /locus_tag="VYO70_000143"
                     /pseudo
     CDS             complement(238..318)
                     /locus_tag="VYO70_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(376..717)
                     /locus_tag="VYO70_000144"
     CDS             complement(376..717)
                     /locus_tag="VYO70_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1712446.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            841..1692
                     /gene="rgg"
                     /locus_tag="VYO70_000145"
     CDS             841..1692
                     /gene="rgg"
                     /locus_tag="VYO70_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1712447.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSMDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            1885..3132
                     /locus_tag="VYO70_000146"
     CDS             1885..3132
                     /locus_tag="VYO70_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1712448.1"
                     /translation="MKLLFRNPAYRILILSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFVGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNAYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            3210..4499
                     /locus_tag="VYO70_000147"
     CDS             3210..4499
                     /locus_tag="VYO70_000147"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1712449.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFATKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            4490..4570
                     /locus_tag="VYO70_000148"
                     /pseudo
     CDS             4490..4570
                     /locus_tag="VYO70_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4840..5154
                     /locus_tag="VYO70_000149"
     CDS             4840..5154
                     /locus_tag="VYO70_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712450.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            5164..5370
                     /locus_tag="VYO70_000150"
     CDS             5164..5370
                     /locus_tag="VYO70_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1712451.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            5370..5567
                     /locus_tag="VYO70_000151"
     CDS             5370..5567
                     /locus_tag="VYO70_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712452.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            5570..6304
                     /locus_tag="VYO70_000152"
     CDS             5570..6304
                     /locus_tag="VYO70_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1712453.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NIIGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            6316..6957
                     /locus_tag="VYO70_000153"
     CDS             6316..6957
                     /locus_tag="VYO70_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1712454.1"
                     /translation="MKLLKNLGWILLALLSFLFIYGFIQGLATASLALGASPYAVTLL
                     YVALAGVYVYGIYKWYQKAPVHIEKSGFNRFIWLPVLVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            6975..7205
                     /locus_tag="VYO70_000154"
     CDS             6975..7205
                     /locus_tag="VYO70_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712455.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            7241..7321
                     /locus_tag="VYO70_000155"
                     /pseudo
     CDS             7241..7321
                     /locus_tag="VYO70_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            7557..9320
                     /gene="aspS"
                     /locus_tag="VYO70_000156"
     CDS             7557..9320
                     /gene="aspS"
                     /locus_tag="VYO70_000156"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1712456.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITESLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVRGVDFKVFSEAPAVKAIVVTGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALVLEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            9298..10239
                     /locus_tag="VYO70_000157"
     CDS             9298..10239
                     /locus_tag="VYO70_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1712457.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLTFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(10449..10529)
                     /locus_tag="VYO70_000158"
                     /pseudo
     CDS             complement(10449..10529)
                     /locus_tag="VYO70_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(10665..11651)
                     /locus_tag="VYO70_000159"
     CDS             complement(10665..11651)
                     /locus_tag="VYO70_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1712458.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(11661..12461)
                     /locus_tag="VYO70_000160"
     CDS             complement(11661..12461)
                     /locus_tag="VYO70_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1712459.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(12722..13564)
                     /locus_tag="VYO70_000161"
     CDS             complement(12722..13564)
                     /locus_tag="VYO70_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1712460.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(13566..14858)
                     /locus_tag="VYO70_000162"
     CDS             complement(13566..14858)
                     /locus_tag="VYO70_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1712461.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(14969..16240)
                     /locus_tag="VYO70_000163"
     CDS             complement(14969..16240)
                     /locus_tag="VYO70_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1712462.1"
                     /translation="MSSKFMKSAAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            16790..18307
                     /gene="malQ"
                     /locus_tag="VYO70_000164"
     CDS             16790..18307
                     /gene="malQ"
                     /locus_tag="VYO70_000164"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1712463.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            18333..20591
                     /gene="glgP"
                     /locus_tag="VYO70_000165"
     CDS             18333..20591
                     /gene="glgP"
                     /locus_tag="VYO70_000165"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1712464.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(21636..22208)
                     /locus_tag="VYO70_000166"
     CDS             complement(21636..22208)
                     /locus_tag="VYO70_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712465.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAMNLLSPGTYLAQLWDKNDAIPNNRRINF"
     gene            complement(23097..23945)
                     /locus_tag="VYO70_000167"
     CDS             complement(23097..23945)
                     /locus_tag="VYO70_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1712466.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNHHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAS
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            24097..24375
                     /locus_tag="VYO70_000168"
     CDS             24097..24375
                     /locus_tag="VYO70_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712467.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            24377..26440
                     /locus_tag="VYO70_000169"
     CDS             24377..26440
                     /locus_tag="VYO70_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1712468.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(26518..27774)
                     /locus_tag="VYO70_000170"
     CDS             complement(26518..27774)
                     /locus_tag="VYO70_000170"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1712469.1"
                     /translation="MHIFDELKDRGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADTVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            27916..30378
                     /locus_tag="VYO70_000171"
     CDS             27916..30378
                     /locus_tag="VYO70_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1712470.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSRARR"
     gene            30443..31354
                     /locus_tag="VYO70_000172"
     CDS             30443..31354
                     /locus_tag="VYO70_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1712471.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            31584..32282
                     /gene="dapD"
                     /locus_tag="VYO70_000173"
     CDS             31584..32282
                     /gene="dapD"
                     /locus_tag="VYO70_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1712472.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            32350..33480
                     /locus_tag="VYO70_000174"
     CDS             32350..33480
                     /locus_tag="VYO70_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1712473.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            33492..34031
                     /locus_tag="VYO70_000175"
     CDS             33492..34031
                     /locus_tag="VYO70_000175"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1712474.1"
                     /translation="MKSELRKQVLQGMKAISQEQKQFIDQTLTERLLHHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDLQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            34015..34692
                     /locus_tag="VYO70_000176"
     CDS             34015..34692
                     /locus_tag="VYO70_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1712475.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(34759..35658)
                     /gene="galU"
                     /locus_tag="VYO70_000177"
     CDS             complement(34759..35658)
                     /gene="galU"
                     /locus_tag="VYO70_000177"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1712476.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKTVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFTGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(35680..36696)
                     /locus_tag="VYO70_000178"
     CDS             complement(35680..36696)
                     /locus_tag="VYO70_000178"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1712477.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKNVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            36908..37283
                     /locus_tag="VYO70_000179"
                     /pseudo
     CDS             36908..37283
                     /locus_tag="VYO70_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(37463..37888)
                     /locus_tag="VYO70_000180"
     CDS             complement(37463..37888)
                     /locus_tag="VYO70_000180"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1712478.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(37956..38606)
                     /gene="phoU"
                     /locus_tag="VYO70_000181"
     CDS             complement(37956..38606)
                     /gene="phoU"
                     /locus_tag="VYO70_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1712479.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKTLLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLSDLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(38621..39373)
                     /gene="pstB"
                     /locus_tag="VYO70_000182"
     CDS             complement(38621..39373)
                     /gene="pstB"
                     /locus_tag="VYO70_000182"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1712480.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWDEVKDNLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(39375..40190)
                     /gene="pstA"
                     /locus_tag="VYO70_000183"
     CDS             complement(39375..40190)
                     /gene="pstA"
                     /locus_tag="VYO70_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1712481.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(40183..41046)
                     /gene="pstC"
                     /locus_tag="VYO70_000184"
     CDS             complement(40183..41046)
                     /gene="pstC"
                     /locus_tag="VYO70_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1712482.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(41164..42039)
                     /locus_tag="VYO70_000185"
     CDS             complement(41164..42039)
                     /locus_tag="VYO70_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1712483.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTTKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(42206..43537)
                     /locus_tag="VYO70_000186"
     CDS             complement(42206..43537)
                     /locus_tag="VYO70_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1712484.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(43538..44245)
                     /locus_tag="VYO70_000187"
     CDS             complement(43538..44245)
                     /locus_tag="VYO70_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1712485.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(44329..44631)
                     /locus_tag="VYO70_000188"
     CDS             complement(44329..44631)
                     /locus_tag="VYO70_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1712486.1"
                     /translation="MTADEIWYFHAESPLTVHMITADGHYEAVTLGLDISKGQQLHYC
                     VPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTRY"
     gene            complement(44618..44839)
                     /locus_tag="VYO70_000189"
     CDS             complement(44618..44839)
                     /locus_tag="VYO70_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1712487.1"
                     /translation="MNKSYFNPLAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINL
                     AGKGRALYTSIYFLLRRNESFTFSPIDSR"
     gene            44801..45358
                     /locus_tag="VYO70_000190"
                     /pseudo
     CDS             44801..45358
                     /locus_tag="VYO70_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(45673..47364)
                     /locus_tag="VYO70_000191"
     CDS             complement(45673..47364)
                     /locus_tag="VYO70_000191"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1712488.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            47500..47946
                     /gene="argR"
                     /locus_tag="VYO70_000192"
     CDS             47500..47946
                     /gene="argR"
                     /locus_tag="VYO70_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1712489.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            47997..50531
                     /gene="mutS"
                     /locus_tag="VYO70_000193"
     CDS             47997..50531
                     /gene="mutS"
                     /locus_tag="VYO70_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1712490.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMENPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            50678..52372
                     /gene="patA"
                     /locus_tag="VYO70_000194"
     CDS             50678..52372
                     /gene="patA"
                     /locus_tag="VYO70_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1712491.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(52702..52965)
                     /locus_tag="VYO70_000195"
                     /pseudo
     CDS             complement(52702..52965)
                     /locus_tag="VYO70_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            53163..54929
                     /gene="patB"
                     /locus_tag="VYO70_000196"
     CDS             53163..54929
                     /gene="patB"
                     /locus_tag="VYO70_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1712492.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            55068..55757
                     /locus_tag="VYO70_000197"
     CDS             55068..55757
                     /locus_tag="VYO70_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1712493.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            55761..55940
                     /locus_tag="VYO70_000198"
     CDS             55761..55940
                     /locus_tag="VYO70_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712494.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            55927..57276
                     /locus_tag="VYO70_000199"
     CDS             55927..57276
                     /locus_tag="VYO70_000199"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1712495.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            57400..58860
                     /locus_tag="VYO70_000200"
     CDS             57400..58860
                     /locus_tag="VYO70_000200"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1712496.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESYENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVAIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENILKEISK"
     gene            58874..59308
                     /locus_tag="VYO70_000201"
     CDS             58874..59308
                     /locus_tag="VYO70_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1712497.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYVNHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVSELIPYWWGEERASKFNER"
BASE COUNT        17357 a        11275 c        12873 g        17860 t
ORIGIN      
        1 gctgacttcg tcagttctat ctacaacctc aaaacagtgt tttgggctga cttcgtcagt
       61 tctatccata acttcaaaac agtgttttga gctgacttcg tcagttctat ctacaacctc
      121 aaaacagtgt tttgggctga cttcgtcagt tctatccaca acctcaaaac agtgttttgg
      181 gctgacttcg tcagttctat ccataacctc aaaacagtgt tttgagctga cttcgtcagt
      241 tctatccata acttcaaaac agtgttttga gcaacctgcg gctagttttc tagtttgccc
      301 tttgattttc attgagtatc aaaaagcaag ccattttcgg cttgcttttt tcatctttaa
      361 aagtgattgg cctgcttaac gaggtggtag tccaagggca atacggccat agcgactgat
      421 tcgtgtgatt ttccaagcag gcgaccaggt aacttcaacc ttgatatctt tgataccctc
      481 gatttgtttc agacctgcca caatttcaat aggcaggctt tcggcgcaat cacaggcagt
      541 atcggtgaag gtcatgacaa tcttgcagag ccccgtttca tccagattga tttcataaat
      601 cagacctaga ttgtaaacat ccaattccac atctgtatca aaaactttct ctagtttttc
      661 gataatttgg tcttgcaagg ccaaagcacg gtcattgatt ttgatatcgt ctctcatagc
      721 agtccctcca cgtgataaat atcctctatt ttctcataat tctgacaatt tggcaagttt
      781 ttgatgaatt ttctgaaaaa tatgataaaa tgaagaaaat acggaatcaa ggggaagtct
      841 atggagcaga ttggaaaagt ctttagacaa ttacgagagt caagaaatat ctcactgaga
      901 caagcaaccg gaggacaatt ttcgccgtct atgttgtccc gttttgaaac aggtcagagt
      961 gagctttcgg tggaaaagtt tctatttgct ctggaaaata tatctgccag tgtggaggag
     1021 atcctctttc tggcgagagg tttccagtat gatacagatt ctgagttgag aaaagaaatt
     1081 acagatgtct tggaaccaaa gaatatagca cctcttgagg acttatatcg cagggagtat
     1141 caaaagcatg cccattctca caacaaacag aaacatattc taaatgccat tattatcaag
     1201 tcttatatga agagcatgga tgaaagggta gagttaacag cagaggaagg aaaagtcctt
     1261 catgattatt tgttttctac cgagatctgg ggaatctatg aactcaattt attttcagtc
     1321 agttctccat ttttatctgt ttctcttttt actagatatg tacgagaaat ggtacgtaaa
     1381 tctgattttc taatggaaat gtctggcaat cgaaaccttt ttcatactat actattgaat
     1441 ggctttttag ctagtattga gtgtgaagaa tttaccaatg cctattattt taaacgtgtt
     1501 atcgaagagc atttctacaa ggaaaatgag acctatttcc gaattgttta tctgtgggca
     1561 gaaggtctcc ttgatagcaa gcaaggtaga gtcaaggaag gccagaaaaa gatggaggat
     1621 gctgtccgta tttttgagat gcttggctgt aataaatctg ccgaatacta tagaaatacg
     1681 actgaatgtt gaatatctgc aaaccaagaa aaaatttgaa gcgatagaac tattgccctc
     1741 tctcaggtca ttacaaaagt tctatcgttt ctttttgctc attttatttg ttgcatatat
     1801 tcaagagttc tccctctcaa atttctaagt gctatactat agtcatactt catatagagg
     1861 ttcgaacaag aagggagatt atctatgaaa ctattgttta gaaatccagc ttatagaatt
     1921 ttgattttat cacgcttctt caatgctttt ggtgcttcga ttttcaacct ggtctttatc
     1981 gtctatgcat cgaccttgtc acaagcatct tttgctgttg ctatggcgaa tattgtcatg
     2041 attcttccga ccctctttac agtttttgta gggattcggg cagattacac gagggacaag
     2101 gtcaaatgga tggtctatag cggtttgttt caggcggttc tattttttct agcagcccta
     2161 gttgttcagc aagctagtct ctttgccttt tctagcctgt gtttgatcaa tgtcattagt
     2221 gatatcatca gtgattttgc aggtggcctg cgtatgcctc ttattaagga aaaagtagct
     2281 gaagatgatt tgatggaggc ttattctttt tcacagttca tcacctatat ttcagctatt
     2341 ggtggtcaag ctttcggagt ctggctctta gctctatcgg tcaacaattt ttctctcgtt
     2401 gcgggaatca atgcctgctt tttcctagta tcagccacta tcctcttttt aggaaaaagc
     2461 aaattgagcc tgtcaatgtc atctgctgat ggtgaaaact taaaaaatga gaagctttct
     2521 atcaaagacc agttcctaac aatttaccga aatttacgtc tcgtttttct taaaagtgga
     2581 cagaaaaact ttggttttat gctctttgct gtcttgctta ttaattcctt gggtggcgct
     2641 ttgggtggaa tttataatat cttctttttg agccattctc tcttgaattt ttcttataca
     2701 gaggcactat ttatcaatca attctgtgtt ttagtagcaa tcatcatcag tagccttacg
     2761 ggcaatgctt attttgggaa gcagtccttg cctagattga tgatgtgggc gaccgtagga
     2821 cttagtctag ttggtttggc taatttattc aatcaagtcg tgcttggttt gctatttctc
     2881 ttctttactc tgtatgtgtc tggtaaagtt caaccaaaga ttagtgccat gctcatgaaa
     2941 aatctagctc cagaggttct agctcgtact agtaattttt taggtctatt gtttacctta
     3001 tccatacctg tgggaacagc ttgtttttca ctagtagccg tatggaacat gcagttgact
     3061 tggatgctat ttgttggtct ttccttgcta gctatttttt taacaattct taatctcaaa
     3121 aatgatatct aaatcctaat ttttttctta ctgttttaat ccctctattt atggtaaaat
     3181 aagactatta agtttaaaga ggattcccta tgaaattaca aaaaccaaaa ggaacgcagg
     3241 atattttacc tgctgagtct gctaagtggc agtacgttga gggctttgcc cgtgagattt
     3301 tcaagcgcta taactatgca gaagtgcgca cgcctatttt tgagcattac gaggttatca
     3361 gccgctctgt cggagataca acggatatcg taaccaagga aatgtacgat ttttatgaca
     3421 agggtgaccg tcatattacc ctccgtccag aaggaactgc gcccgttgtc cgttcctatg
     3481 tggaaaataa actcttcgcc ccagaagtgc aaaagccaag caagttctac tatatgggac
     3541 ctatgttccg ttatgagcgt ccacaggcag ggcgcttgcg ccaattccac cagattggtg
     3601 ttgagtgttt tggctctagc aatccagcta ccgatgtgga aacaatcgtt atggcagccc
     3661 attttttgaa ggaaatcggt attcaaggtg tcaaattgca cctcaacact cttggaaatc
     3721 ctgagagccg tgcagcttac cgccaagcct tgattgacta tttgacaccg ctcaaggaga
     3781 ccttgtctaa ggatagccaa cgtcgcttgg aggaaaatcc tcttcgtgtc ttggactcta
     3841 aggaaaaaga agacaaggtg gcagtagaga atgcgccgtc tatcttggac tttcttgatg
     3901 aagaaagcca agctcatttt gatgctgtgc gtcagatgtt ggaaaatctt ggagtagatt
     3961 acatcatcga taccaatatg gtgcgtggtc tggactacta caaccacacc attttcgagt
     4021 ttatcacaga gattgagggc aatgacttga cagtctgtgc gggtggtcgc tacgatggtt
     4081 tggttgctta ctttggaggc cctgaaactg ctggatttgg ttttgggctt ggtgtagagc
     4141 gtctgcttct catccttgaa aaacaaggcg tggccctccc tatcgaaaat gccttagatg
     4201 tctatatcgc agtcttgggt gatggagcaa atgtcaaagc cctagaacta gtccaagccc
     4261 ttcgccaaca aggtttcaaa gcagagcgtg attacctcaa ccgtaagctc aaagctcagt
     4321 tcaagtcagc cgatgtcttt gcgactaaga ccctcatcac cctgggagag agcgaagtcg
     4381 aaagcggaca agtgacggtc aagaacaacc aaacccgaga agaagtgcaa gtgtcacttg
     4441 agacaatcag ccaaaacttt tcagaaatct ttgaaaaact aggattttat actcaataaa
     4501 aatcaaagag caaactagga agctagccgc agattgctca aaacactgtt ttgaagttat
     4561 ggatagaact gacgaagtca gctcaaaaca tggttttgag gttgtagatg aaactgacga
     4621 agtcagtaac catacctacg gcaaggtgaa gctgacgtgg tttgaagaga ttttagaaga
     4681 gtattaatga tagataaagt ggtcaggaac ttactcctga cctttttctt ttgcaaaatg
     4741 acaaaaatca taaacttttt gacaactata attgtcaaaa agaaaaagat atgttacaat
     4801 gaattcaagt taagaaatag gaagcagaaa ggagttataa tgtgggtact aggatttata
     4861 ctatttatga ttttctttta ttctaataat tctaaaaaaa tcaagaaact agagaataaa
     4921 atcaaaaaac ttgagcgaaa agagaaagga aacgcagaaa tgtcgagatt attacaagaa
     4981 atgattggaa aggaaccaat tataacggga gtgtatattg ggccagataa ctgggaagtt
     5041 gtggatgttg atgaggaatg ggtcaaacta cgtagtgttg ataatacggg aaaagaaaaa
     5101 ttcaagttgc aacgtattga agatatccaa accgttgaat ttgacggaga gtaggtgtgt
     5161 aacgtgcaac taaaaaatcg tctaaaagag cttcgagctc gcgatggtct caatcaaacc
     5221 gacctagcca aactggcagg ggtttccaga cagaccatta gcctactaga acgggatgag
     5281 tacaccccat ccattatcat tgccctgaaa atatctcaaa ttttcaacga aacagtcgaa
     5341 tcggtatttc gtttggagga ggacgagtga tgaacaagta taaagtgatt tattatgtag
     5401 tggtcatagc tttattagtc agcgtatttc tactgattgg gatggaccta agctggttta
     5461 atccctatca aagcgaccaa tttgtttggg tctactttgc tctcatccca gtaactgaat
     5521 ggattgaaaa gaaatccaaa aatctagcaa gtgaaaaagg agaatgaata tgaaaaagaa
     5581 acgtagattg ttatttttga tgtctattgt cttggggggg ttcttgggca tgtttgtagg
     5641 gatgtttaag gcacgtgtcg aatcccacga aattatttta gatgtaaaag ccttgatgcc
     5701 atggatatca gctatttgtt tactgatagg tttcattagt atgtttttga ctttcaattt
     5761 cttaaagaaa agcagaaaat ttcattcctt gtatcaagag gaaatggatg acgatctgaa
     5821 tgaaacctat tatgtgcaaa tgtatcggaa tcttgagttt ggaaccattg cttttaatat
     5881 tataggtgta gcgattccat tggctatttt tatttcatta agtgaggtga ttatattgca
     5941 tacaaaccct caaacatttt tcctttcttt cttactcttt gtggtattct tagtcgctca
     6001 aaaatctctt tttaaaacca ttgcgattgt tcgtcagttt gatttggaat ttttcgctac
     6061 accaaaggat gtcttgaact atataaattc ttatgatgaa ggggagcgtc aggctaattt
     6121 ggaacagagt tttcgaattt tattccaatt acaccaatat gtcttaccag ccttatatat
     6181 ttttcttatt atcatttctt tcttgacagg agagattcag ttactagctt tcttgcttgt
     6241 aggagccatc catgtttata tcaatgtgat gcagttacct atggtaaaac gttatttcaa
     6301 ataaaggagt tatctatgaa attacttaaa aaccttggct ggattcttct agcccttcta
     6361 tcctttttat ttatctatgg ctttattcag gggctcgcga ctgcgtcgct tgctttaggc
     6421 gcttcaccct atgctgttac tctactctat gtggccttgg ctggagtata tgtgtacggc
     6481 atttacaaat ggtatcagaa ggctcctgtt catattgaga agagcggctt taatcgattt
     6541 atttggcttc ctgtcttggt ttggttctta tctctggtcg ttcaattctt cttgccagat
     6601 gatccttcag taaatcagca aatagcgaca gacttgacct tgtctcaacc acttttctca
     6661 ttctttgctg tggttatttt tgctcctttg acggaagaga ttgtttttag agggatgtta
     6721 gcacgctatc tctttcctaa gcaggacaat agtaaacgaa ccctgatttt tcttctggta
     6781 tctagtcttc tatttgcctt gattcatttt ccaggtgatg tgcaacaatt ttttgtctat
     6841 tttagccttg gttttagttt gggtttggct tacgttagca gaaaaggtct ggtctacagt
     6901 atttctcttc acgctttgaa taatttagtc ggctttttga tgattctcat gctataatag
     6961 agtcaggagg tcacatgaaa cgagtaattt tattagcagt gattcaggca gtcgttctat
     7021 ttttcatcat tggagcgcta gcttatgcct tcaaaggcga tttcttctat aactatctag
     7081 cagttgtctt tgctcctatt gcaggtgtac tgcgttttgg gacggcttac ataacggaaa
     7141 ttgtcttgcc tcgaaaggca gccgaaatcg ctgaaaagcg taaagcaggc aaaaattcaa
     7201 aataaaagag tccggtgaag ttcatcggat ttttgtatta tactcaatga aaatcaaaga
     7261 gcaaactagg aagctagccg caggttgctc aaaacactgt tttgaggttg tggatagaac
     7321 tgacgaagtc agctcaaaac atggttttga ggttgtagat gaaactgacg aagtcagtaa
     7381 ccatacctac ggtaaggcga cgctgacgtg gcttgaagag attttcgaag agtattagcg
     7441 ctaattttta gggctttcac ctgactttta aagactggca aacttttctg atgattttca
     7501 tgaagagcct gcgcttttat ggtaaaatag taacagaata aaagaggaga gaaataatga
     7561 aacgtagtat gtatgctggt cgtgttcgtg aggaacacat cggacaagaa ataaccttga
     7621 aaggatgggt tggccgtcgt cgtgaccttg gtggtttgat ctttatcgat cttcgtgacc
     7681 gtgaaggaat catgcagttg gttatcaacc ctgaaaaagt atctgcagag gttatggcaa
     7741 cagctgaaag ccttcgtagc gaatttgtta ttgaggtgac tggtcaggtc gctgcgcgtg
     7801 agcaagccaa tgataagttg ccaactggtg cggttgagtt aaacgtgaca gctcttattg
     7861 tgcttaacac agctaagaca acaccatttg agattaagga tggcattgag gcaaatgacg
     7921 atacacgttt gcgttaccgt taccttgacc ttcgtcgtcc agaaatgttg gaaaatctta
     7981 aacttcgtgc caaggtgacc cactctatcc gcaactactt ggatgagttg gagtttatcg
     8041 acgtggagac accattcctt tctaagtcaa cgcctgaagg ggcgcgtgat tatttagtgc
     8101 cgtctcgtgt taataagggg catttttacg ctcttcctca aagtccacaa atcacgaaac
     8161 agctcttgat gaatgctggt tttgaccgtt actaccaaat cgttaaatgt ttccgtgacg
     8221 aggacttgcg tggagaccgc cagcctgaat ttactcaggt cgacttggaa acgtccttcc
     8281 ttactgagca agaaatccaa gatattacag aaagcttgat cgcgcgcgtg atgaaggaaa
     8341 caaaaggcat cgaagtaacg ctaccattcc ctcgtatgaa atacgatgat gctatggctc
     8401 tttacggttc tgacaagcca gatactcgtt ttgacatgtt gcttcaggac ttgacagaag
     8461 tggtccgagg tgtagacttt aaagtctttt cagaagcacc tgctgtaaaa gcgattgtgg
     8521 tcacaggagc tgcggacaac tattcacgta aagacatcga caagatgacg gaagtagcca
     8581 aacagtatgg tgccaaaggt cttgcttggg tcaaggtggt tgatggagaa ttaaacggac
     8641 cagttgccaa gttcttgact ggtatccaag aagaattgac aacagcgctt gttcttgaag
     8701 ataaggactt ggttctcttt gtggcggata cgcttgaagt ggctaatgca acactgggtg
     8761 cccttcgtgg acgtattgct aaagagcttg gcttgattga taatgataag ttcaacttcc
     8821 tttgggtggt tgactggccg atgtttgaat ggtctgaaga agaaggccgc tacatgagcg
     8881 cccaccatcc tttcacactt ccacaggaag agactgctca tgaattagaa ggtgatttgg
     8941 ctaaggttcg tgccattgct tacgatatcg tcttgaacgg ttatgagctt ggtggtggta
     9001 gccttcgtat caaccaaaaa gaccttcaag aacgcatgtt caaggctctt ggtttctcaa
     9061 ctgaagaagc caatgaccag tttggtttcc ttcttgaagc catggactat ggtttcccac
     9121 cacacggtgg tttggctatc gggcttgacc gttttgtcat gttgcttgct ggagaagaaa
     9181 atatccgtga agtcattgcc tttcctaaga acaacaaggc aactgaccca atgacacaag
     9241 ctccatcaac agtcgctctc aaacaactag aggaactcag cttacaagta gaagaagatg
     9301 aaacaaacaa aacgaattaa gcggtggcgc tattatctgc gccgctttgc ttatcagata
     9361 aaaattttac gtgtcttaca aagtatctct cgagaaaagt atgatgagaa gatttcggcc
     9421 tctctggtct atggtttttt atcagcagta gcagttaatt tctttttcca accagggcat
     9481 gtgtattcga gtggtgcaac aggtctggca cagattatct ctgccttgag taatcactgg
     9541 tttggttttc atattccgat ttcgctaacc ttctacgcca ttaacttccc tttgatggtc
     9601 ttagcttggt atcagattgg ccataagttc accgtcttta cctttatcac ggtatctatg
     9661 agttccttct ttatccagtt tgtccctgtg gcaaccttga cggaggatcc cattatcaat
     9721 gccctttttg ggggtgttgt tatgggtttg gggattggtt ttgctcttcg aaacaatatc
     9781 tccagtggtg ggacggatat cgtcagcctg actattcgca agaaaacggg taagaatgtt
     9841 ggtagtattt ctttcttggt aaatggaact atcatgctga tagcaggttt gacctttggt
     9901 tggaaatacg ctctttattc tatgattacc atctttgtct ctagccgtgt gacagacgca
     9961 gtctttacta agcaaaagcg tatgcaggcc atgattgtga caaatcatcc agagaaggta
    10021 attgaaaaaa tccataaaaa attgcaccgc ggagcaacca tgatccatga tgcagaagga
    10081 acctataatc acgagagaaa ggcagtttta atcactgtca ttacacgtgc agagtttaat
    10141 gaatttaaac agattatgac acaagtggat ccaagctcct ttgtctctgt ctcggaaaat
    10201 gttcatattc taggaagatt tgttgagata gataattagt gattaatact cttcgaaaat
    10261 ctcttcaaac cacgtcagcg tcgccttacc gtatgtatgt tactgacttc gtcagttcta
    10321 tctgcaacct caaaaccatg ttttgagctg acttcgtcag tttcatctac aacctcaaaa
    10381 ccatgttttg agctgactcc gtcagtttca tctacaacct caaaaccatg ttttgagctg
    10441 acttcgtcag ttctatccac aacctcaaaa cagtgttttg agcaacctgt ggctagcttc
    10501 ctagtttgca ctttgatttt cattgagtat aaaaagatct tgggtaacaa ctcaatttca
    10561 gtagaaaagg aagtaaatct tcccacaata aaacgcatag tatcaagttt tttcaatacc
    10621 tgatagtatg cgtttttgct tttaacgact ttttgcccag tctcttattt ttcgataatt
    10681 ttgtgggcga tcaattgacg gtaacaaatt tgtttattgt ttttattatc attaataatc
    10741 tggagaatag tttcaaggga aacacgacca agctctaaac tattgatatc gacataggct
    10801 gccaagttga gcttgggatt aaccgagtca aagctgagaa cagggacatc cagctggtgt
    10861 ttggcaatat agttacaaac accttcagct aggaggctat cggttgtgat gatagcatca
    10921 atttgtggat cgtgcttgaa taatcgcttg ctaaatttat agcccttttc ttccagaaac
    10981 tcgtcggcaa agtagatgcg attgttgtca gtggtaagtt tgtaatgttt aagcgcctgt
    11041 tcatagcctg ttaaacggtc tttggtcacg aagagctttt tacttcctcc gataaaggca
    11101 atgcgtttgc agcctttttt gatgaaatat tcagtcgcat caaaaccagc ttgaacattg
    11161 tcgttgtcga caagtgggat gaaaggagat agagatttac ctaagataag gaaggggaac
    11221 tgttcttctg cgacgagttt tacgagaggg tcttcttctt gggcatagag aaagattagc
    11281 ccatctacac gcttgccgta gaccatttgt gaaatagcgt tgagacgctc cttctcatct
    11341 ttccctgttg ctatctgaat ggcatagtgg ttttcagatg cgacttgaga gatgccacgt
    11401 agaaccgatg gaaagaaagg attctggtag aaggcgtctg agtcatcagg aagaactaat
    11461 ccgataacct gagtatagct gcttaccaag ctacgagcgt tgaggtttgg gtggtagttg
    11521 agttccttca tagctttgcg aacgcgtttt tttgtttcgt cgctaatggt tgatttattt
    11581 tgaataacac gggttacggt tgaaggcgaa acaccagcag ccttggccac gtctttaatc
    11641 gtaacgggca taaaaatctc ctatttatgg taatttggat cacggaaatg tgttttataa
    11701 aagatagtga ccagatacag aacaaaaaga atattttgta cagtaatcag ggttgtcata
    11761 ttttggccaa ataatcccaa aataagtgta atcagagtcg gcaatcctaa acagttcaag
    11821 ataaagtgat agcactcttt aaaggttcta aatgaaaaga ggcgtgattt cttagtgata
    11881 taaaggagaa gactagctcc tagagagacg ataaagaaat tcaaaccaaa gaggaagctc
    11941 gcaccgagaa ctaggaagag gctgatatag acacgatttt gttggtacca gtctttagaa
    12001 attgcttggg tcaagctgtc tttgcttttg aaactctcag tctgaatggc tcggtaagag
    12061 atgcgggtca gttccttgct ttccttgctg atgactagct catttgtatc gaaatgcagt
    12121 tgcaagtcct taggtaattc cttgatttga cttggaccaa tcacaacaga aggggcttga
    12181 ctagctgttc cagtataagt taatgtgcca tcgacaattg tagcatgttc agagagatcc
    12241 tggacaacct catttgtcag aggttcatag acattatcga taaaagtttc tagcggatag
    12301 gtctcctggg agctgttttg gatagcaatg ggtaccatag acaagctgat aaggaagata
    12361 ctggtaaaga gaagttgaaa ccagttgagc tcgaaacgtt tggacagggg cttacgaaac
    12421 ccccaaatac ttgaaaaata ggaaaatgga tatggaagca taggtatctt tctaaaaggt
    12481 gttttctata tgagtattat tatatagaga aatgcgcttt atttcaagtc tagggggtaa
    12541 attgcttgct acaaggatat tatttacagt gacaatcgct aatagtcaat gaaaatcaaa
    12601 gtgcaaacca ggaagctagc tgcaggttgc tcaaagcaca actttgaggt tggagataaa
    12661 gctgacgtgg cttgaagaga ttttcgaaga gtataaaatg aaaaagggtg gcggggataa
    12721 attatccctt gtcgccacca cttgtaagtc ctgaaacaaa gttcttttgt aggaagaaga
    12781 agagaataca gattggaagg gcgatgagga tagcacctgc tgagaagtag gcaatcttca
    12841 agtttttcgc attgttaacg aaggtttgga gacctacggc aacagtaaag tattctttct
    12901 cacgaagcaa gaaactagag aggatgtagt ccccgaaagg tcccatgaag gcccagagag
    12961 cttgtacggc aaccattggg cgaacaagtg gtagaacaat ttgccagaag cggcggaagt
    13021 gtcctgcacc gtctagtttt gcagattcgt ctaaagacat tggcactgta tcgaagtagc
    13081 ctttcatgag ccaagcattc atcgggatac caccaccaac gtagaggaag atgaggaacc
    13141 agttgtggtt aagggcgttc aacataagcg ccataacgaa gaaggctgtc aaagcggcca
    13201 tagttggcac catttggatg atcaagaaga agaccaaact ttgtttacga gccaagaagt
    13261 tgtaacggct gtaagcataa ccagcaagta cgatgatact tgtttgaaca gccatggtaa
    13321 ttaaggcgat aatcaaagtg ttgaggtacc aagtaccgta caaggtttca gtgaagaggc
    13381 ctttaaagtt atcaaaattg aggtcgatat tagtatctag tttaaaggct gagacgttac
    13441 ctgctttaaa ggctgacatg atggtaatca acagtggata gataattaca attgatagac
    13501 caatcaggta aaggtaagta aggctttgag tcagtctacg tttgagttta attgagttat
    13561 tcatcttaga cgtcctccat atcaaatgcg tgtagtttct tgaatgcgat catagagatt
    13621 gagatgacaa tgatagagat aatcaaggta acagctgccg ccattgagta ttgaggagat
    13681 gtacctgttg tcaaacggta gatccatgag atcaagatat cggttgaacc agctccacct
    13741 ccgacactac caggtcctcc accattgaag aggtacatga tagagaagtt gttaaagttg
    13801 aaggtgtatt ggctaatcaa agtaggtgcc gcaacagcca aaatcattgg gaaagtgatg
    13861 ttgcggaatt tttgccaagc gttggcaccg tcaatataag ctgcttcgta aaggtcgtta
    13921 ggaatagatt gcaagatacc caaagtcaaa acgtagatgt atgggaatcc gagccaacct
    13981 tgcatcataa tcaaggcaat cttagtccaa gttgggtctg ttttccaagg aataagagct
    14041 ccatcaagga aaggaaggaa tttagccaag attggcaata cttgagtgtt gatagcaccg
    14101 acactatcgt taaacatgtt tgagaatgtc aagatagtga tgaaggctgg gacagcccaa
    14161 ggaagaagga aaataacacc aaagatacgt tttcctttga taaatggttg gttggcaatg
    14221 atagctgtga agataccaat tacgatttgt aaagtagaag ctgccaaagc ccaaatgata
    14281 gtccaagaaa gaacagaacc aaaggcagaa cggaaggtac tcaagctcca aatgtttgta
    14341 aagttggtca aaccaaccca gtccaacaac ttgtttggtg gcaagtgttg gaagtcgtag
    14401 ttggtaaagg cgatcatcaa ggttacgata actgggaaga taatcgcgaa ggtcatggca
    14461 acataagatg gaatgatcaa gaggtaaggg aagccatttt cataaatccc tttgatcatg
    14521 tctttgagtg tgcgtggaac tggaattcca ttgttaatgc gttttgcaat cgtatgtgca
    14581 tctttgatat ttgagaaata aaagagtaca taaacgatta caaagattag atggaaggca
    14641 ccacgaatca gcataaagag ggaattatca cgacctggtt tgtcaccaag agtgatgagg
    14701 ttgctcaatt caggggttgc aagtgctagg aagtaaagga caaatacgat ggttacacca
    14761 aggaagataa aacctttggc tttttgttta ttgtaaatct gtcctaaccc aggaatgata
    14821 gacagcaggg ctgctttact aggttgttgc ttttccatat taactccttt cataggatac
    14881 tactttttta taagaaagct aaattatttg tgtctctatt gataaattca aagggggatt
    14941 tgatttccac cccccttgaa caaatttttt attcaccaaa tttttgtttg attgtttctt
    15001 tgatcaatgt tacagcatcg ttagcagctg ttttagcatc tttttgacca cttacagcat
    15061 caaagagcat atttttcgct ggatcccaaa ctgcagacat ttgagagatg tttggcagtg
    15121 gttgagtgtt cttgaactgt ttgataacag ctgttgtcaa ctcatcgttt ttaccttcag
    15181 cgtatgaacg agcctcagta ttagctggga tttcgttagt cttatcatat aatacttttt
    15241 gttgttcagt tgcaacaagg aagtctacaa atttttgaga agcttcaagg ttcttaacgg
    15301 cttgaggaat gacccaagct ttaccaccac cgaatgcagc atattctttt ccatttggaa
    15361 gagttgggat agttgcaact ccgtagttta ctttagcatc tttaaaggct tgagctttcc
    15421 aaggtccgtc gatgatagca gctgttttac cttcttggaa ttgagtttgg attaagtttc
    15481 cagcaccttc tgtatcttgc atacctttag gccatttttc gtaccaagat ttagcgtagt
    15541 tgatacctac gatagaaccg tcgtttgcaa gaccgatgtc tttagcgtct ttaccgtttt
    15601 ggccaaagac gtaagcaccg ttaccggcaa gaagtccata tgtatagtag aagtttgtcc
    15661 agtcagctag gaaggcagta gttttaccat cttcaccagc gaatgcgtat ttgctatctt
    15721 tagcaaggtt ttccaagtca gcaaatgttt ttggagcatc tttcaccaag tctttgttgt
    15781 agtacataac aagtgactcg ataacggcag gagcaccgta aactttacca ttagcagctg
    15841 ttacaagaga tttagttgtg tcgtctgttt tagcaccatc gctcaatttc acttctgaaa
    15901 gttgtccgtc agaaccaagg ctacctacac ggtcgtatgg agccatcata acatcaggga
    15961 cattaccaga ttggttgtca agagaaagtt tatcaagacc tcctagagca tcaccagttt
    16021 taagagtgac ttttactcca gcttcttttt cataagcttt agcaacctct tcaatatagc
    16081 tcttatatcc ctcgtctaca tatacagtga gttctttgac ttcagatgaa ccagaatcag
    16141 caggcttatc agcagttttg cttccgcaag ctaccaaaag caagctagca agtgtaacag
    16201 ttccaagcac cgcagcgctc ttcataaatt tagatgacat agtgtattcc tcccaaagaa
    16261 tagcaagttt tattgataag gaaacgcaaa cgttttcctt tatgagctta gtatagcaca
    16321 aatagaaaac ggttgcaagt attttttgta aaaattttaa aaaattttta agcttgattt
    16381 gatagcataa ttttgcattt taatagaaga aaagtatgaa atagataaga atacagtatt
    16441 tttaataaaa atatatggaa tatgtctaaa gagggaaacc aggaggggct acctccctgg
    16501 ttctaatccc tatctatttt ctatggacgt ttgtgctttg ttacatataa attcaagctc
    16561 cctaaggagg ttttgtactt tgtagactgt ctcgtgagag tcaaacctta ggattagata
    16621 gttttaagtt atctttgcta aaatgagtta gtctccttaa aaataatcaa aaaagttttt
    16681 taaaaacgct tgcaattatg cttgaaaagg agtatactta taagtaacgc aaacgtttgc
    16741 gtctgcaaaa tacgcaacgt tccattattt taacacacga ggtgctatta tgaaaaaacg
    16801 tcaaagtggt gtgttgatgc acatctcttc tcttccagga gcttacggaa tcggatcatt
    16861 tggtcaaagt gcttacgact tcgttgattt cttggtccgt acaaaacaac gttactggca
    16921 aatccttcca ttaggagcaa ctagttacgg ggattctcct taccaatctt tctcagcctt
    16981 cgcaggaaac actcatttta tcgatttaga tatcttggtg gagcaaggtt tgttggaagc
    17041 aagtgacctt gaaggagttg actttggtag cgatgcgtct gaagttgact atgctaaaat
    17101 ctactatgca cgtcgtcctc ttttagaaaa agcggtgaaa cgtttctttg aagtcggaga
    17161 tgttaaagat tttgagaaat ttgctcaaga caaccaatca tggcttgagc tctttgctga
    17221 gtatatggct atcaaagagt attttgacaa tcttgcttgg actgaatggc cagatgcaga
    17281 tgctcgtgct cgtaaagctt cagcacttga aagctatcgt gagcaattgg cagacaagtt
    17341 ggtttaccac cgtgtgactc aatacttctt cttccaacaa tggttgaaat tgaaagctta
    17401 cgctaacgac aaccacatcg aaatcgttgg ggacatgcca atctacgtag cggaagattc
    17461 aagtgatatg tgggcaaatc cacatctctt caaaacagat gtcaatggta aggctacttg
    17521 tatcgcagga tgcccaccag atgagttttc tgtaactggt cagctttggg gtaacccaat
    17581 ctatgactgg gaagcaatgg acaaagacgg ctacaaatgg tggattgaac gcttgcgtga
    17641 aagcttcaaa atctacgata tcgttcgtat cgaccacttc cgtggcttcg aatcttactg
    17701 ggaaatccct gctggttccg atacagcagc acctggtgag tgggtgaaag gtccaggcta
    17761 caagcttttt gcagccgtta aggaagaact tggtgagcta aacatcatcg cagaagacct
    17821 tggcttcatg acagatgaag tgatcgaatt gcgtgaacgt actggcttcc caggaatgaa
    17881 gattcttcaa tttgccttca acccagaaga cgaaagcatt gatagcccac acttggcacc
    17941 tgctaactca gttatgtaca caggaacaca cgataacaat acggttcttg gttggtaccg
    18001 taacgagatt gatgatgcga ctcgtgagta catggctcgt tacacgaacc gtaaagaata
    18061 cgaaacagtg gtacacgcta tgcttcgtac agtattttca tcagttagct ttatggcaat
    18121 tgcaactatg caagatttac tagaattgga tgaggcagct cgtatgaact tcccatctac
    18181 ccttggtgga aactggtctt ggcgtatgac tgaagatcaa ttgacaccag ctgtcgagga
    18241 aggtttgctt gacttgacaa caatttatcg ccgaattaat gaaaatttgg tagatttaaa
    18301 gaaataagac aataatcagg agacaactaa acatgttatc actacaagaa tttgtacaaa
    18361 atcgttacaa taaaaccatt gcagaatgta gcaatgaaga gctttacctt gctcttctta
    18421 actacagcaa gcttgcaagc agccaaaaac cagtcaacac tggtaagaaa aaagtttact
    18481 acatctcagc tgagttcttg attggtaaac tcttgtcaaa caacttgatt aaccttggtc
    18541 tttacgacga tgttaaaaaa gaacttgcag ctgcaggtaa agacttgatc gaagttgaag
    18601 aagttgaatt ggaaccatct cttggtaatg gtggtttggg acgtttggct gcctgcttta
    18661 tcgactcaat tgctactctt ggtttgaatg gtgacggtgt tggtcttaac taccactttg
    18721 gtcttttcca acaagttctt aaaaacaacc aacaagaaac aattccaaat gcatggttga
    18781 cagagcaaaa ctggttggtt cgctcaagcc gtagctacca agtaccattt gcagacttta
    18841 ctttgacatc aactctttac gatattgatg ttactggtta tgaaacagcg actaaaaacc
    18901 gcttgcgttt gtttgacttg gattcagttg attcttctat tattaaagat ggtatcaact
    18961 ttgacaagac agatatcgct cgcaacttaa ctctcttcct ttacccagat gatagtgacc
    19021 gtcaaggtga attgctccgt atcttccaac aatacttcat ggtttcaaac ggtgcgcaat
    19081 tgatcatcga cgaagcaatc gaaaaaggaa gcaacttgca tgaccttgct gactacgcag
    19141 ttgtccaaat caacgatact cacccatcaa tggtgattcc tgaattgatt cgtcttttga
    19201 ctgcacgtgg tatcgagctt gacgaagcaa tctcaattgt tcgtagcatg actgcctaca
    19261 ctaaccacac aatccttgct gaagcgcttg aaaaatggcc tcttgaattc ttgcaagaag
    19321 tggttcctca cttggtacca atcatcgaag aattggaccg tcgtgtgaag gcagagtaca
    19381 aagatccagc tgttcaaatc atcgatgaga gcggacgtgt tcacatggct cacatggata
    19441 tccactacgg atacagtgtt aacggggttg cagcacttca tactgaaatc ttgaaaaatt
    19501 ctgagttgaa agccttctac gacctttacc cagaaaagtt caacaacaaa acaaacggta
    19561 tcactttccg tcgttggctt atgcatgcta acccaagatt gtctcactac ttggatgaga
    19621 ttcttggaga tggttggcac catgaagcag atgagcttga aaaacttttg tcttatgaag
    19681 acaaagcagc tgtcaaagaa aaattggaaa gcatcaaggc tcacaacaaa cgtaaattgg
    19741 ctcgtcactt gaaagaacac caaggtgtgg aaatcaatcc aaattctatc tttgatatcc
    19801 aaatcaaacg tcttcacgag tacaaacgcc aacaaatgaa cgctttgtac gtaatccaca
    19861 aataccttga catcaaagct ggtaacatcc ctgctcgtcc aatcacaatc ttctttggtg
    19921 gtaaagcagc tccagcctac acaatcgctc aagacattat ccatttaatc ctttgcatgt
    19981 cagaagttat tgctaacgat ccagcagtag ctccacactt gcaagtagtt atggttgaaa
    20041 actacaacgt tactgcagca agtttcctta tcccagcatg tgatatctca gaacaaatct
    20101 cacttgcttc taaagaagct tcaggtactg gtaacatgaa attcatgttg aacggagctt
    20161 tgactcttgg tactatggac ggagctaacg tggaaatcgc tgagttggtt ggtgacgaaa
    20221 acatctacat ctttggtgaa gattcagaaa ctgttatcga cctttacgca aaagcagcat
    20281 ataaatcaag cgaattctac gctcgtgaag ctatcaaacc attggttgac ttcattgtta
    20341 gcgatgcagt tcttgcagct ggaaacaaag agcgcttgga acgtctttac aatgaattga
    20401 tcaacaaaga ctggttcatg actcttcttg acttggaaga ctacatcaaa gtcaaagagc
    20461 aaatgcttgc tgactacgaa gaccgtgacg catggttgga taaagtcatc gttaacattt
    20521 ctaaagcagg attcttctca tctgaccgta caatcgctca gtataacgaa gacatctggc
    20581 acttgaacta atactcttcg aaaatctctt caaaccacgt cagctttatc tgcaacctca
    20641 aagcggtgct ttgagcaacc tgcggctagc ttcctagttt gctctttgat tttcattgag
    20701 tataagatac aaatttatac taatacattt tgtaaaaaag cgagtttcga ttgaaattcg
    20761 cttttttaaa tgctgtggat ttgggtcaat cttgtctaaa aatagggaaa tcctagatac
    20821 agtgaaggct ttaaatgctg gtttttactg tctttagcct tatatttttt cgtagttggt
    20881 tacctcatat ctcttgtatt cgcttacata aagtattata atataattgt aggaaagaag
    20941 gtgtttttat gatatacaca cttaaattgg tgttgtttat tacctttctt gtaataagct
    21001 tgttacctga taagattttt ggaaaaaata aaaaaatttg gaaaatagtt tttgcaatat
    21061 tgacggcagt ggcagcattg tcatttatgt actaagttat tttaagaatg tagggaaata
    21121 aaccctacat tctttttagt tttttctgtt ttctaaattc tatttatcca agcgcttcaa
    21181 catttcttgc ttcttcgctt caagttctgc acgcttttct tcgatttcgg catgtttttt
    21241 ctcgagttca gaacaacttg catcattgct aaattctttt cgccatcagg agatagggtg
    21301 agtcgacatg tctattactc acccaaagca gtcctacaaa gcaggaattt tctgttactt
    21361 ttttggaaat agtaacgttt atacagcttt gacacttcgt atcaaagcgc caaacacact
    21421 ccgagggatt tacagaaagc agaaaaggaa tgatctggta taagatcatt ccttttctct
    21481 ctttttcttt aagtaattat atacaatgta cgacgaagtc gtcattgcaa tgctgatcca
    21541 ccacctaaag ggaactttaa acaagattga taagataaag aatataaaca acgaaaatac
    21601 gttataccca attaatttta ttgtatatct catgattaaa agttaatcct tctgttgtta
    21661 ggaatggcat catttttatc ccataattgt gctaaataag tccccggtga taataaattc
    21721 atagcgaatt ctaaagcaac atcatttaca aaccaactac ctagatatct agaaattgct
    21781 gaacgaatag cactttttgc tgcatgtttt ccttttactt taattagatt tgcaaggcct
    21841 gcagtagttc ctcctaatgc taaagctatt gcagtatcta atagagcacc catttgatta
    21901 actgtaatac cttgccaagc tgctctaaat ggagagtatg taggtgggat tgtataatcg
    21961 ccttgtaatt gtcggttaat tacttctttg atccattgtt gtgagacgtc tggatgaaaa
    22021 gattggattt cgtttgcaag tgtattgatt tgttcttctg ttagagaagt gacaggttga
    22081 agttccatat ttgtttcaat ttgtgatact tgttcagaag cgtatacagc tgaaacactt
    22141 ggaatcgctg atacaattaa cacaattgac gtcaaaaaaa ccgaaataaa tttcattaat
    22201 ttgttcatga acttttctcc tttttatttg tatctgctta cattttatca tatactgtta
    22261 ttatagtcaa aaaaatatgc tattatgtta aaaaaatatt tttcaaaata taaatggacg
    22321 gatttatttt ggattttatt tgttattttg acctgcctct atattggtaa ccatgatttg
    22381 tttactctca atcatcaaga attctctttt cgtggtagcg tttggggtct ggtactggcc
    22441 ttatatcact tactattcat tgataagttt gttatatcga atcgaaaata aagattagag
    22501 ctatgcttga ctggctactt ttaggattga ttttggagga agattttgtc tctattattt
    22561 attattttaa atttatttat tttgtataag atctattctt tgaggcggga aaaatcgaaa
    22621 tacttgattt atacggccta tatcatattt ggggtaaatg tagtatatgg tattcaatgg
    22681 ttattaaaag aactgatttc aactatttcc ccttaatgtg tatggaaact ataacaaaac
    22741 gcataagatt aaggtttgtg tacctgttct tatacgtttt tgtcatattg aagattttcg
    22801 gtaagttttc tacttttgcg aagtctagtt tatactcaat gaaaatcaaa gagtaaacta
    22861 gccgtaggtt gctcaaagta cagctttgag gttgcagata aaactgacga agtcagctca
    22921 aaacatggtt ttgaggttgt agatgaaact gacgaagtca gctcaaaaca tggttttgag
    22981 gttgtagatg aaactgacga agtcagctca aaacatggtt ttgaggttgt agatgaaact
    23041 gacgaagtca gtaaccatac gtacggcaag gcgacgttga cgtggtttga agagtattag
    23101 aatgctttcc caaccaaaat ctctgcttcg atggtaatct ctgtcagttg gctccagtca
    23161 atcttgctct ggtcaacgtg aaagaggaga ggagtcatac tgagtagggc ttggagttgt
    23221 tctgctgtga tagtcttagt cagagaggca gtttgactag ataggatggt aaagtgttcc
    23281 tggaaatgct ccttgatatc ttggttagaa tactccttgt ttgtcagctg gtcctgtact
    23341 ctttgacgga tttctttgag gtgattttca gttgggataa cctttatcaa gataccgtct
    23401 ttggataaaa cgcgacgaaa ttctccatag ttggcaggtg agaagatatc aagcaggata
    23461 tccatgctag cgtcttttat aggaaggcgt gccaagtcgc caacgaacca attgactgcc
    23521 cagttgggtt cacttttagc agcgatttgg actgaatctt tggagatgtc aaaggcatag
    23581 aaagtttttt cagagtgact ttcttgtagt ttgcgagaat agaatccttc accacaaccg
    23641 atatccaaaa ttgttgtggt agtttttgag cttgcaagca agtcagatac agcatctaag
    23701 atagcttggt aaaagccggc ttctaggatt tgttgacggt tttgaaaatt ttccttgtca
    23761 tagttagcag attgcttgat ttgaggtgct agattgacat agccaaattt cgccaagtca
    23821 aaagaatgat ggttgcagca cttgaaatta gtctctaaca gagtcagatt ttcttgacag
    23881 ataggacagg caaaggcagt cgcagaagca aaacgctgaa gtttgggctt gagatttgta
    23941 ttcatagttt aagtgtatca aaagaggcaa atgaaagcaa ctttaggaat aagtagatgg
    24001 gagattcagt agaaataaaa ctaattctcc aatttataga atgaaattgc ttttatatct
    24061 aaattgctat ataataatga taaggaggaa ataagtatgt taaaagaagt attaaccgtt
    24121 gcaaaagttg cgaaaaaatc ttcattattt ttgggtggtg tcgcatttgg tacccttggt
    24181 ttgaaaatct tagcaagtaa ggaagctaaa aaaggttatt ctaaagcttt ggctaaggct
    24241 tacaagttga aagacgagct agatgcatct gtttctgtcg tgaagcaaca tggagacgat
    24301 gtcttgcaag atgccaaata tttgtacgag caagagaaaa aagaagagca attagatagc
    24361 cttataggtg aataatatgt cttttaaagt gctacataga ggataccaac atatccgact
    24421 atcatcttct ttttcactta ccttggatat tcaagactat cttcgttcct tggcgagaga
    24481 tgaaaagggg attgagtcta tccagtttta catggatcaa cagcacttta ctctacgcat
    24541 aaaagaaggc ttttctgtat tagataatgc agaagccttt ttaaaaagaa ttgataaagg
    24601 gaaagtttct gagttgatga ctcttcccat tcgtagagaa gagagtgctt attctattgt
    24661 ttcaggtgca gcggttaagc gtgtactttt tcgtagtttt gtgccgtatc ctattcgcta
    24721 tatatggact tgttatcagg ctttgggtta tattagagaa gcctatcaaa cactagcgcg
    24781 taaggaacta acgatggaag tcttggactg ttcggctatt ttattgtctt tgtttatgaa
    24841 ccaatccaag acagctagca atatcatgtt tatgcttgat ttggggaatc atttagatca
    24901 gtggtccttg aaaaaaactg caacagattt agaacagagt cttcttgcaa aagagagcga
    24961 tgtattccta gtacagggcg atacggttgt tagtatcaag agttccgatg ttcaaatagg
    25021 agatgtcttg atcttatctc aaggaaatga aattctgttt gatggacaag tagtttcagg
    25081 tttaggtatg gtcaacgaaa gttccttgac aggagagagt tttccagttg aaaaaagaga
    25141 gtctgatttg gtttgtgcaa atacagtatt agaaactgga gagttacgca ttcgtgtaac
    25201 agataatcag atgaacagcc gtattttaca gctgattgag ttgatgaaga aatctgaaga
    25261 aaacaagaaa acgaaacaac gctatttcat caagatggcg gataaagtcg tcaaatataa
    25321 tttcttgggg gctgggctga cttacctatt gacaggttct ttttctaagg ctatttcttt
    25381 cctattggtc gatttctcct gcgctttgaa aatctctact cctgtagctt atttgacagt
    25441 tatcaaggaa gggttgaacc gtgaaatggt gattaaggat ggagatgttc tggagaaata
    25501 tctggaagtt gatactttct tgtttgataa gacaggaaca atcacaacta gttatcctat
    25561 agttgaaaag gtgttacctt ttggagacta tagtgaggaa gatattctca gaatcagtgc
    25621 ctgtcttgag gaacacattt atcatcctat tgctaatgcc atcgtcaagc aagctgagat
    25681 agagggaatt gaacatgagg aaatgcatgg gaaactccaa tatatcgcaa gcaaggggat
    25741 caaatctcat atagatggcc aaccagttct tattgggaat tatgtcttga tgcaggatga
    25801 gcagattcat atcagttcag aacaaaatgc tttaattgaa gagtacaaga gtcactacaa
    25861 tctcttattc ttggcttatc aaaatgaatt gattggaatg ttctgcattc atactccttt
    25921 gagaaaagaa gcaaaaacag ccttggataa acttaaggca caggggaaaa aattgattct
    25981 ggcaacagga gacaccttga ttaggacaga ggaattagtc aaagatttgc cctttgatca
    26041 ggtctataca gacttgaaac ctgatgggaa atttgagtta gtagagaaac tgcagaaagc
    26101 aggtcacact attttgatgg ttggagatgg cttgaatgac tcagcggctt taactctatc
    26161 agatatcggt gtggtgatga atgagagtgc agatatttct aagcagatga gtgatatctt
    26221 attgttagat aatcgtttgg atttcttcca agagttggat tcgttatcat catctttgca
    26281 aacactcatc aagaaaaata ttcaggatac cgttgtcgta aatagtagtt tgattggctt
    26341 tggcttattt aattggttca gtccttcaaa tctctctatc ttacataatc taacaacctt
    26401 gcgcattgtc ctgcgtagcc tgtctattaa aaatagatag atgagagtta ttaacagcaa
    26461 aaaggagtta cctttctcga ggttaactcc tttgtttata gatagatgtt gaatagttta
    26521 gtaagtcaat acaaagtatt ttttcttccc acgacggata acagtcagtt cattttctaa
    26581 cttatcagcg tcactcaaga catagtcaag gtcttggatg cggtcgccgt ttacgtagat
    26641 ggctccgttt tggacgtctt cacgggcttg gcgttttgag ttaactatac cagatgagac
    26701 gagcagttcc acgatattgt tgttttcgtc tgcctgtact tggtagtttg gcacaccacg
    26761 aagtccttgt ttgagctctt tgacagaaag gtttttgatg tttcctgcaa agagttgctc
    26821 agtgatgtta agtgcttctt tgtaggcttc ttctccgtga acaagtgtaa caacttcacg
    26881 agccaagact ttttgagcca agcgttcgtg tggcgctgct tcaaattgtt tacgaatatc
    26941 ttcaatctca tcaagtgaca agaaagtaaa gattttcaag aagcgaacag tgtcagcgtc
    27001 catcacgttc atccagaatt ggtacatttc gtatggagaa gtcttttcgg gattaagcca
    27061 gacggcattt ccttctgatt taccaaattt cttaccagtt gcatctgtga ttagtggaac
    27121 agtgataacg tgaccagtct tgtccgcctt acgacgaagc aattcggtac cagctgtcat
    27181 atttccccac tggtcagaac caccgatttg aagagtgaca ttatggtctt ggttaaggac
    27241 gaagaagtcg tacccttgca tgatttggta agcgaactca gtgtaagaaa ttcctgtttc
    27301 gatccgtttt ttaacagatt ccttactcat catgtagttg accgtgaagt attttccaat
    27361 atcacggagg aagtcaatga agctgatgct gccaaaccag tcgtagttgt tgaccatgac
    27421 agccttgttt tcgccatttt caaagtcaag aaaacgagaa agttgtcctt ggatagactt
    27481 gacccagcca tctactgtgt cttttgtttg gagactacgt tcagcatctt tgaaggacgg
    27541 atctccgatg agacctgtag caccgccaac gagcgcataa ggtttgtgac ctgctagttg
    27601 caagcgacga cttgtcaaga ttgcgacaag gtggcctagg tgaaggctgt cagcagttgg
    27661 atcgtagcca gtataataag aaacttgacc ttcttctagg gctttacgca aagcttcttc
    27721 atcagtcgtt tgaaatatca aaccacgatc ttttagctca tcaaaaatgt gcatgtgtct
    27781 tttctccttt ttaaaattat tgtttctacc tattgtatca caaacttggg caatagccta
    27841 gcagaatagg gaagaaagtg gctattttat tgaaaatttc ccttttgtgg tataatagat
    27901 agagtgagga aatccatgca aaatcaatta aatgaattaa aacgaaaaat gctggaattt
    27961 ttccagcaaa aacaaaaaaa taaaaaatca gctagacctg gcaagaaagg ttcaagtacc
    28021 aaaaaatcta aaacattaga taagtcagcc attttcccag ctattttact gagtataaaa
    28081 gccttattta acttactctt tgtactcggt tttctaggag gaatgttggg agctgggatt
    28141 gctttgggat acggagtggc cttatttgac aaggttcggg tgcctcagac agaagaattg
    28201 gtgaatcagg tcaaggacat ctcttctatt tcagagatta cctattcgga cgggacggtg
    28261 attgcttcca tagagagtga tttgttgcgc acttctatct catctgagca aatttcggaa
    28321 aatctgaaga aggctatcat tgcgacagaa gatgaacact ttaaagaaca taagggtgta
    28381 gtacccaagg cggtgattcg tgcgaccttg gggaaatttg taggtttggg ttcctctagt
    28441 gggggttcaa ccttgaccca gcaactaatt aaacagcagg tggttgggga tgcgccgacc
    28501 ttggctcgta aggcggcaga gattgtggat gctcttgcct tggaacgcgc catgaataaa
    28561 gatgagattt taacgaccta tctcaatgtg gctccctttg gccgaaataa taagggacag
    28621 aatattgcag gggctcggca agcagctgag ggaattttcg gtgtagatgc cagtcagttg
    28681 actgttcctc aagcagcatt tttagcagga cttccacaga gtcccattac ttactctcct
    28741 tatgaaaata ctggggagtt gaagagtgat gaagacctag aaattggctt aagacgggct
    28801 aaggcagttc tttacagtat gtatcgtaca ggtgcattaa gcaaagacga gtattctcag
    28861 tacaaggatt atgaccttaa acaggacttt ttaccatcgg gcacggttac aggaatttca
    28921 cgagactatt tatactttac aactttggca gaagctcaag aacgtatgta tgactatcta
    28981 gctcagagag acaatgtctc cgctaaggag ttgaaaaatg aggcaactca gaagttttat
    29041 cgagatttgg cagccaagga aattgaaaat ggtggttata agattactac taccatagat
    29101 cagaaaattc attctgccat gcaaagtgcg gttgctgatt atggctatct tttagacgat
    29161 ggaacaggtc gtgtagaagt agggaatgtc ttgatggaca accaaacagg tgctattcta
    29221 ggctttgtag gtggtcgtaa ttatcaagaa aatcaaaata atcatgcctt tgataccaaa
    29281 cgttcgccag cttctactac caagcccttg ctggcctacg gtattgctat tgaccagggc
    29341 ttgatgggaa gtgaaacgat tctatctaac tatccaacaa actttgctaa tggcaatccg
    29401 attatgtatg ctaatagcaa gggaacagga atgatgacct tgggagaagc tctgaactat
    29461 tcatggaata tccctgctta ctggacctat cgtatgctcc gtgaaaaggg tgttgatgtc
    29521 aagggttata tggaaaagat gggttacgag attcctgagt acggtattga gagcttgcca
    29581 atgggtggtg gtattgaagt cacagttgcc cagcatacca atggctatca gaccttagct
    29641 aataatggag tttatcatca gaagcatgtg atttcaaaga ttgaagcagc agatggtaga
    29701 gtggtgtatg agtatcagga taaaccggtt caagtctatt caaaagctac tgcgacgatt
    29761 atgcagggat tgctacgaga agttctatcc tctcgtgtga caacaacctt caagtctaac
    29821 ctgacttctt taaatcctac tctggctaat gcagattgga ttgggaagac tggtacaacc
    29881 aaccaagacg aaaatatgtg gctcatgctt tcgacaccta gattaaccct aggtggctgg
    29941 attgggcatg atgataatca ttcattgtca cgtagagcag gttattctaa taactctaat
    30001 tacatggctc atctagtaaa tgcgattcag caagcttccc caagcatttg ggggaacgag
    30061 cgctttgctt tagatcctag tgtagtgaaa tcggaagtct tgaaatcaac aggtcaaaaa
    30121 ccagggaagg tttctgttga aggaaaagaa gtagaggtca caggttcgac tgttaccagc
    30181 tattgggcta ataagtcagg agcgccagcg acaagttatc gctttgctat tggtggaagt
    30241 gatgcggatt atcagaatgc ttggtctagt attgtgggga gtctaccaac tccatccagc
    30301 tccagcagtt caagtagtag ttctagcgat agcagtaact caagtactac acgaccttct
    30361 tcaagggcga gacgataatt ctctaaatga agtggccaat caatggattg ccactttttt
    30421 cctttcgtgt tacaataagg gtatgaatca gtatcagaaa aagattgtta acggaaaaat
    30481 ttattcgctc ctatccggct taatatgggg aatctgtgga attttaggag agtacttctt
    30541 tactcattat caggtgtctt cgggctggat tacctctatg cgtttgacac tggcagggag
    30601 tcttgtactc atttggtctg caatacaatt aaaatcgcaa gtgctagata tttggcgaga
    30661 caagaaaaat tacctgccct ttttagccta tgctattttg gggatttttt cagttcagta
    30721 ttttttctat ctctgtgtag aatactcaaa tgctacgaca gcaactattt tacagtttat
    30781 tagccctgtc tttatcctct tttataatcg cttggtttat caaaaacgag cgtcaaaaag
    30841 cgctgttttc tatgttttgg ttgccatgct gggtgtttgc ttgatggcga caaagggaga
    30901 tctctctcag ttatccatga cgccgctagc tcttataaca ggtttgctga gtgccatggg
    30961 tgttatgttt aatgttatct tgccccaacc ttttgctaag cgttatggtt ttgttcctac
    31021 ggttgggtgg gggatgattt tggcaggttt gtttagcaat gtcctctcgc cggtttatca
    31081 gctttccttt actcttgata tttggagtat cttgatttgc ctcattatcg ctttctttgg
    31141 aacggctttt gcttttttca tttccatgaa ggctgtgtcc ttggtttctc ctttggtggt
    31201 tgccgttatc agtgccagtg aacctctctc ttctgctctc ttgagtgttt tgttcttagg
    31261 attagtagtg gattggtccc tccttctagc tatagccttg attattttac ccatgatatt
    31321 tttgtctata gaagaagcga aagaaagtag ataaaaagtc ttttcttaat tctaaaagtg
    31381 tgctatacta gaatagtcaa taaaacacgg ggagatagtt gtgaagagtg tttttaggtt
    31441 gtatcggtag gggcttgctt ttaccgacag cacgttttat ctcacatccc taaaaaatca
    31501 tacctaaaaa cagacaaaaa ggtttcaaat ttgaggcctt ttttggtaga ataggtatca
    31561 ttataacgaa ccaggaggca cctatgactg ctacaaaaat gaacgctcaa gaaattatcc
    31621 aatttatcgc caatgctgaa aagaaaacca gtgtcaaagt aacctttgag gggcaactcg
    31681 caactgctgt gcctagctct gttgtcaaac taggaaatgt cctattcgga gactggaagg
    31741 acgtggctcc gcttcttgaa ggtttggtag aaaatcaaga ttatgttgtc gagcaagatg
    31801 ctcgtaattc tgcagttcct ttgctagata agcgtgctat caacgctcgt atcgagccag
    31861 gtgctattat ccgtgaccag gtggaaattg gtgacaatgc tgttatcatg atgggagctg
    31921 ttatcaatat cggtgctgaa atcggtgctg gaaccatgat tgacatgggt gccatccttg
    31981 gtggccgtgc tatcgttgga aaaaatagcc acgttggtgc aggtgcagtt ttggcaggtg
    32041 tgattgagcc agctagtgct gaaccagtcc gtgtcggaga caatgttctt atcggtgcta
    32101 atgcagtggt tatcgaagga gtccaaatcg gtagtggttc agttgtcgct gcaggagcta
    32161 tcgttaccca agatgtccca gaaaacgtgg tagtagcagg tgttccagct cgtatcatca
    32221 aagaaattga tgcccaaacc caacaaaaaa cagcgctaga ggatgcgctt cgtaccttgt
    32281 aattgtaaaa gtaaaaaaga ggcggaaccc tttttccagc ctctttctgc tatatcggag
    32341 gacagataga tgttagattt gattcagact agacgagctt tacaccagat tccagagatt
    32401 ggcttggagg agttcaagac tcaggcttat ttgctggatg tgattgagaa attgactacg
    32461 ggcaaggatt ttgttcaaat tcgtacttgg cggacaggga ttttggtcta cctgcaggga
    32521 agtcagccgg agcgaaccat tggttggcgg acagatattg atggcctgcc tatcgtcgaa
    32581 caaacaggtc taccttttgc ttctcaacac caaggtcgca tgcatgcctg tggccatgat
    32641 tttcatatga ccattgcctt aggctgtctt gagcgcgccc ttgaggagca acccaagaat
    32701 aatctgctct ttctatttca gcctgctgaa gaaaatgaag ctggtggtat gctcatgtat
    32761 gaggatggtg cttttggaga ttggttgcca aaccaattct atggtctcca tgttcgtcca
    32821 gatttgaaag tcggccagat tgcgactaat actcatacac tctttgcagg aacttgcgag
    32881 gtgaagatcc gtttcaaagg caaaggtggc cacgcagctt ttcctcatga agccaatgac
    32941 gccttggtgg cggctagtta ctttgtgacc caagtgcagt cagttgttag ccgcaatgtc
    33001 aacccaatcg agggagcagt ggtgaccttc ggcgtttttc aagctggaac caccaacaat
    33061 gtcatcacag acacagcttt tttacatgga actattcgtg ccttgacaca ggacatgagt
    33121 ctcttggtgc aaaagagggt aaaaacagtt gcagaagggg ttgcagctgc ctttgatatg
    33181 gaagtcgaag tggaactcaa acaaggtgga tacctacctg ttgagaacaa tccagccttg
    33241 gcgcgtgaac tgatggactt ctttgacgaa aaagacggaa tcgagttgat tgatatcgaa
    33301 cctgctatga caggcgagga ctttggttat ctcctttcaa aggttgatgg cgtgatgttt
    33361 tggctaggta tcgatagtcc ctacgctctt catcaccctc aaatgagtcc taaggaagaa
    33421 gtcttagcta ttggggtggc tgcggtgtct agtttcctga aaaagaaggc agcagaatag
    33481 agggcttgtc tatgaaatcg gaattacgca agcaagtctt gcaaggaatg aaggctatat
    33541 ctcaagaaca aaaacagttt atagatcaaa ctttaaccga gcggctttta caccatccct
    33601 tctatcaaga agccaaggtc atcgcaacct acctctcttt ttctcatgag tttcaaacgc
    33661 gggaactgat tgagcaggcg ctgaaggacg gcaagaaggt tttaataccc aaaacttatc
    33721 ccaaggggcg catggacttt gtagtctatg atctgcagca gttggtaaaa actgcctttg
    33781 ccttactgga gccacaggga gatttggaag tggtggatgt atctcagatt gatttgattc
    33841 atgttcctgg cctggctttt acgacggatg gatatcggat tggatatggt ggaggttatt
    33901 atgaccgcta tctggaacat ttttctggtc atactttgag tacggttcat ccttgtcaaa
    33961 ttcaggactt tatacctgaa aaccatgata ttcctgttca ggaggtatta attgatgaag
    34021 gaaatctttg atagacgtta ccctgtgacg agtttcttcc tcttagtgac ggccttggta
    34081 tttttactaa tgttggtcac tgcaggcggg aactttgaca gggcagatac attatttcga
    34141 tttggagcca tgtatgggcc agctattcgc ctctttcccg agcaggtttg gcgtctcttg
    34201 tctgccattt ttgttcatat tgggtgggaa catttcattg ttaatatgct ttcactttat
    34261 tatcttggaa ggcaggtaga ggagattttc ggttctaagc agtttttctt tctctatctt
    34321 ttatcaggaa tgatgggcaa tctctttgtt tttgtattta gtcctaaatc cttagcagca
    34381 ggcgcctcta cctctcttta tgggctattt gccgcgatta ttgttcttcg ctatgcaact
    34441 cgcaatcctt atattcaaca gctagggcaa tcctatctga cactttttgt ggttaacatt
    34501 attggaagtg ttctgattcc aggaatcagc ctagcaggcc atatcggtgg tgcagttggt
    34561 ggcgcatttc tagcagttat ctttccagtt agaggagaaa aacggatgta taataccagc
    34621 cagagattag gagcggtagt cttgttcgta ggactcgcca ttttgctttt ctacaaggga
    34681 atgggaatgt gacgaagttg aaagaaaatt tgtgtaatga aaaaagataa ggcatttttt
    34741 gaaccttatc tttttatttt attccttctc agtcaattct tttccaagtt ggatgaggta
    34801 attcttcaaa tcatctttga cttgtggatg tttgagggcg tagtcaatag atgttttcat
    34861 aaagccaaac ttatccccaa cgtcgtaacg agctcctgta aattcacggg caaaaacacg
    34921 ctgtgtttta ttaagagtat cgattgcatc agtaagttgg atttcatttc cagcaccagg
    34981 agcttgcttt tcgagaatct caaaaatttc aggcgtgagg aggtagcgtc cgataatagc
    35041 aaggtcgcta ggagcgtcct ctggagctgg tttttcaaca aaggtttcaa cactgtaaag
    35101 accatctttt ccttcgcctt gcggagcaat aaccccgtaa gcagatactt cgtcatgagg
    35161 gactggcatg acagcgatag tagacgcgtg ggtacgctcg tagtcatcca tgagttgttt
    35221 ggtaagtgga acagtctttt cgtctgtgat atccatcaag tcatcaccca gcataacaac
    35281 aaaaggttca tttccaacga aagccttggc ttgcaaaaca gcatcgccga gaccacgtgg
    35341 atgcgtttgg cggataaaat gcaggcgcat gccagttgtt tcatcaacta gcttcagaag
    35401 atctgttttc cctttttctt tgagattata ttccaattca aagtttgaat caaagtggtc
    35461 ctcaatagaa cgttttgatt taccagtgac aactagaata tcttcaatac ctgatttgag
    35521 agcttcttcc acgataaact ggatagttgg tttgtctacg attggcaaca tttctttggc
    35581 aagggccttg gttgctggta aaaatcgagt tcctagtcca gcagcaggga tgactgcctt
    35641 tctaactttt gatgtcataa aaatcctttc tatagagggt taagaccact cattttctgc
    35701 tttaaattca ttgttcatga tgtcataaat ggcatctttg atattggttc cgtgataaat
    35761 aacttggtaa atagcctgtg taatgggcat atagactcca agttcttggg ctagttcata
    35821 ggctgctcga gtcgttgaaa ttccttcgat taccatgccc atattagctt ctatatcagc
    35881 tagggattct cctcgtccga gagcatctcc agctctccag ttacgagagt ggatggaagt
    35941 tcccgttacg atcaaatctc ccacaccaga taagccgcta taggtcaatg gactggcccc
    36001 gagtgctacc cctaggcggg tgatttctgc taaacctcga gcgatgatgg ctgccttagc
    36061 attatcacca aatccaagac catgtaaagc tccagcaccg acagcaataa tatttttaag
    36121 agcaccagca gtttcaaccc cgataacatc cgtattggta taaagtcgga agtagtgatt
    36181 actaaatagc tcctgaacgt attgagctgt ttgtaaatct ttagaagcag cagttattaa
    36241 agttaggtca cgcacaatgg tctcttctgc atgactaggc cctgaaacaa cgacgatatc
    36301 actacggaga tgttcaggaa tttcttcttc aagaatggtt gataatcgtt tatggctatc
    36361 aggttctaat ccctttgatg cgtgcatgat gataacctta tggtccaagg tttgtgcaac
    36421 ttgctgggca acaagtcgtg tcacttttgt tgggacaaca aacaaaatcg catccacatt
    36481 tttcaatgtt tctgctaagt cggtgtaggc aatgatattt tcgtctagaa cgacatcttt
    36541 aaagtagtgc ttattagtat ggtgtgtatt aatttcattg atttgctcgg gaagatttcc
    36601 ccaaatacgt acctcgtgtc cattgtcatt taagacttgt gaaagggcgg ttccccaaga
    36661 accaggcccc aagacggcga cggtttgttt ttccatgatt tcctccttga tagatttctt
    36721 tctcttattc tatcatattc taggggattt tgcacttgca ttactggggt atggtggaaa
    36781 aatacacaaa gaccgagatg aagggataaa ctcttagtct ttagttaaag tgagaagaaa
    36841 tctgatagat agtttgattc agtatatgct tataatagag gtaaacaact caggaggttc
    36901 ttgtaggttg cgagagtttg gcgaaaaaat taaaagatta cgtttggcta aaaaaatcag
    36961 tcgttcagaa ttttgtggtg atgagtctga attaagtatc cgtcaattaa ttagaattga
    37021 aaatggagaa tccagaccaa tactaacaaa gttaaaatat attgctgaac gtttggaggt
    37081 tgaagattac aagttgatgc caagttatat agagttggat aaggaatacc tagaattgaa
    37141 gtatttcttg atgaggactc ctacatacga agatgaaact atcgcccaaa agaaagagag
    37201 tgtttttgct aagatttttg aagagtatta tgataggcta cctgaggaag aaagatttat
    37261 catcccaaat tattcatatc tagcactagc gaactacaca gttcaaaaat taccagaaaa
    37321 gctagttgaa atactgtcct tctggtgatt ttgcttaatt tctgatgtta gagacataat
    37381 tgaggagatt tgtcttgatt ttttcctaaa aaagggcaga ctcctccctt ggtttgatgc
    37441 tcgattttta aaataaggtg acttattctg ttaggcatgt tttcccctgc cctctagctc
    37501 tatatggaac agggagtatc agatttatct ggcagattgt atcaaataag gcttgaaact
    37561 gttttgaata ggcgtataga cttgagaatt tgagaatatg tcgtctagca gtagagacat
    37621 attttccggc aatatgaagg aagagacgtc gaaaacgctt gatagtcaag gcctttactt
    37681 catcaccagc tagctgcttt aaaaagaggt agagattata agccagacag cccatcatca
    37741 tacgtacttc attcttaatc atggtcgaac tatctgtctt atccccaaag aatcctgctt
    37801 tcctttcttt gataaagttt tcagcattcc ctcgttcacg atagaattga aacagctcct
    37861 cactatctac actgcttaaa ttggtcaaat gaactttttt acaaccataa aaaagagcac
    37921 ttaatcgtac tcttctcttt taaggattag ttgaattaat tcaaatccac tagttctcct
    37981 gtttctaggt agactaggcg ttcacaaatg ttagcaatgt aatcagcgaa gcgttccaga
    38041 tgccctatga tataaaggta ttgtgttcca ttgggaattg aggtttcttg gtctttcata
    38101 agtccaatga tttcctttga taaggcataa taatattggt caatctgttc atctttttga
    38161 gcaatactaa tagcttttga ggcttggtgc aaaggaaagg caaccaataa atcggatagc
    38221 atgctgaggg ataatttacc catttggtgt aactgttctt cgtcaggggc tagttgattt
    38281 tcttttagtt gcaaaacagc tttagcaatg cctgccatat ggtctcccat acgttcaagg
    38341 tctgaacaag aagacatgat gctaatcaca aatcgaaggt cagacacttg tggctgctgc
    38401 aaggccaaca aacgggcaca ggtcaattcg atagcgcttt gaccttggtt gatagcatga
    38461 tccttattga taattagctc tgccatctcc ttgtctttgg aggctaaggc cagtaaggtt
    38521 tttgaagctg tttcaaggac aagttgccct agtcctaaaa aggattgttc taattcatgc
    38581 aattctaagt caaattgatt tctcataggt ttttccttcc ttatccgaac cgtcctgaaa
    38641 tatagtcttc tgtgcgctga tcttttggat tggtaaacac gtcaacggtg tctccaaatt
    38701 cgcaaatttc tcctgttaag aaaaaagcag ttttatctga aatacgtgaa gcttgttgca
    38761 tgttatgggt aacaatgata atcgtataat ccttttttag ttgctgaatg aggtcttcaa
    38821 tttttaaagt ggagataggg tctaaggctg aagtcggctc atccattaac agaacatcag
    38881 gttctactgc taaagctcgc gcaatgcaaa ggcgttgctg ctgaccgcct gataaggaca
    38941 tggcactctt tttaagatta tctttgactt cgtcccaaat ggctgcccct tttaaagatt
    39001 tttccactaa ggcatctaat tgttttttgt ctcgaatacc atgtgtcctt gggccataag
    39061 ccacgttatc atagatagac atggcaaagg gattaggctg ttgaaaaacc atccctacac
    39121 gcttgcgtag ctgattaagg ttgaatttgc tactataaat atcttgctca tctaagagga
    39181 cttggccttc aatatggcaa gaaggaacca aatcgttcat ccgattaagg gtttttagaa
    39241 aagttgattt accacaacca gatgggccta tcaaggcagt aatctgtctt tctggtaatt
    39301 gaatcgaaat attttttaag gcttgaaaat ccccgtaaaa taagtctagg tgtctgactg
    39361 aaaatgttcc catactagga agctcctttc acaagttttc gagataataa gcttgataga
    39421 gtatttatca ttaaaacagt aataatcaaa atcacaccgg tagcataggc ttcattgaca
    39481 tgtagcccct cacttgacag catatacata tgcagggcta gagaacgacc tgaagacatg
    39541 agactacttg gcgtattggt agaggtacct aatgtataca tgagggcagc tgtctcacca
    39601 acgatacggc caatagctag tatcactcca gctaaaatac ctggcatggc aactggtaga
    39661 acaattctaa aaacagtccg taacttacct gccccaagtc cataacttgc ttgacgcatg
    39721 ctatcactaa cagataaaag ggcttcttct gttgagcgaa taatgactgg caacaccatg
    39781 atgactgagg ttaagattcc tgataacaga gagtattgaa aacctaagaa gactacaaag
    39841 aagagcatgc caaacagacc aaaaacaatg gaaggaatcc cagacaaggt atctgaggcc
    39901 aatcgcataa ttttaacaca aagggaatct ttttttgtat attccacaag ataaaaacca
    39961 gcaaaaatcc ctatgggcaa ggctaaaaga agagcaccaa aaaccagaat aacggtggaa
    40021 ataatcgctg gcataaggga aatgttctca gaagtataag tccaagaaaa gagggataga
    40081 cttagatgag gtaagccttt gatgaggata aaaccaatga ttaaaaagag agagccaaag
    40141 gttaaagctg aaaaacaata aacgagaagt tttagcaggt atttactcat aagatgattt
    40201 tcctttcaag taggcaaagt aggcattaat caagagaata aggaaaaaga gaactgctga
    40261 ggttgcaata agggcttccc tatgctgacc tgatgcgtaa gccatttcca gaacaatatt
    40321 ggttgttaag gttctggttc ctgaaaagag tccacttgga ataatcggct ggttgcctgc
    40381 caccaaaata actgccatgg tttcacctac tgcgcgaccg attcctaaaa taactgctga
    40441 taaaatacca gatctcgcag ctggcaagat gacactaaaa atactccgtt catgactagc
    40501 tcctagagcc aagctaccag aataatacgt tttgggaact gttcggatag cagattctga
    40561 caaactgata atggttggca aaatcattat tcctaatagt aacgaagcgg ttaggacact
    40621 catgccattt cctaaaaagg ttctaatcca aggcaccaat aattgtaggc cgaaaaaacc
    40681 ataaacaata gatggaatgg ccgccatcaa gttgatagct gattttaaga agccatagac
    40741 gggctttgga caataataaa ccataaacac cgatgtcaag atgcctgttg gcaccccaat
    40801 cacaatcgct cctaaggtaa ttaataagga accaacgatc attggtaaaa taccatagct
    40861 tgccggaatg ttcgttggcg accaatcact gcctaataaa aaacgggcaa agccgtagtt
    40921 agccatgaaa ggtaagccat tactaaaaat aaagaaacag attagcaaaa tagctacaac
    40981 agctactgtt gcactcatga aaaaaattgc cctaaaaact gcttctttga aggcttgttt
    41041 tatcacatct tgtcctttct agtgaagaaa gtaagggaga tacgacacct ccctacttgc
    41101 cttctttatc ttattgtacg atgaaacgtc tgcatctctt tagagattta tggagcaaac
    41161 attttattta atcttgtccc aggtggttaa tttgccacta aaaacgtctg caagttcagc
    41221 catactgact tggcttgcct tattgtcatt attgaccaca acagcaatac cgtctaaagc
    41281 aatagcatca tgggtgagac tcttaccttc ttcaggagtt aattccctag aaaccatacc
    41341 aatatcagcg gttttctcct taacagcggt aatacctgct gaagatccat tagaggtaat
    41401 atcaatcgta acttctggat tttctttttt ataagcttct gctaattttt ccattaaaga
    41461 agatactgaa gtggaaccta caacagacaa cttgcctgat aagtgttggc ttgtatattc
    41521 tgtggtttcg gttttagctt caataaattt attatctgtg accacttgtt gaccttgttt
    41581 ggagtggata aagctgataa aatcttgacc tagcttggaa agattagaag accaaacaat
    41641 gttgaaggga cgttgaagag ggtattcacc atctaaaact gtgtctcgac tagccttgac
    41701 accatcaatc tctaaagcct tgacagattt cgttaaagat cccaaggaga tgtagccgat
    41761 agcattagca ttcccttgaa ctgctgagag aacaccttct gtactatttt gaatcacagc
    41821 tgttttggta gtgttgtcaa tttttttatc accgtctttt ttgagaatcc ctgtgatttc
    41881 tgtgaaggca ccccgtgttc cagagccatt ttctcgtgaa atcacctcaa tcgttcctga
    41941 agctgactgt ttggaagcag ctgactgatt gccacaggca acaagcccaa atcctgataa
    42001 gccaatggct gcaagagtaa gcattttttt gaatttcata ataatcacct ttatctctat
    42061 gtatttttct tgtgtaggct tactacattt atagtctaac aagtctttgt aaaggtttat
    42121 ccctgattca tgtaaagatt gtgtaaagaa tcaaaaaaag ccacttttga aaaatggctg
    42181 ctcctaaaaa tagctttaaa aattattagt cctgtgcgaa agattggtta ggaagaaaaa
    42241 tcgtgaagca actgcctctg ccaagctgac tcgtcaccgt gacttggcca cctaataatt
    42301 gactgagttc tttgacaatg gcaaggccaa gaccagtgcc accagtttgt ctgcttcgac
    42361 ctttattaac tcggtaaaaa cgttcaaaaa tacgatcctg ctctaattga ctaataccaa
    42421 tccctgtatc tgatacagaa atcttaatgc cttcgttcac cttttgggtc ttgacctcaa
    42481 tttttccccc ttgttcagtg taacggatgg cattggataa aagattgagt aagatttggg
    42541 aaagtaattg actatctgat acgagggtga catcatctgg cacctgcacc tttagctgta
    42601 aatccttctt cttgagctga ggttgcaagc tttgagtcaa atcctgtaca aattctgcca
    42661 aagaaagggt cgtccattgt ataggcattt gttgagcctt agataaggta agaagatgct
    42721 caacaatatg ctcaagacgc aaactttctt tgtaaataat gtctagaaag tcatccttga
    42781 gcgcttcttc ttcagctgac atccccttaa tggtttcagc aaagccctta atcgaagtaa
    42841 ctggtgtcct caattcatgg gaggcatttg agacaaaggc taaatttaac ttttcataag
    42901 ttctaatcgt tgttaaatca tatagcaaga cgagcacagc ttccacagat tgggtggggc
    42961 taaaaacggg aactgctgtc acttctaaaa tcaagtcacc ctcatgaaac ccacttactt
    43021 cttgttttaa ccttgttttt tgatcaaagg cttggtgaac taaattccga atatccatcc
    43081 gtttgaggtc atcaagtgaa cttatgtcgc cgtccacatc gggaaaataa tgaggcagag
    43141 agcgactgga taataacatc tgaccttgag cggaaactaa aaacgtcccc atggttaggt
    43201 gcgacagaag aacctccatt gtttcggcta gatccttgta ttgctgatcc tgttgggaga
    43261 ctttggtttt taggccagac acatactgag ccaaagactt taagtcttct tgcccttttt
    43321 ctaaaaagta ttcactactg gtcaagagag gttggtgcaa ggtctcaaaa gcaacttccc
    43381 atttccaaag gcaaaagagc cagtagccac ctagtcccaa agaaagggct agaagaaaga
    43441 gaccgatgcc tttactgatc caagttaatg ccatccctgc aatcagaatg aggctaacac
    43501 ttagattgac tagccaaaat tgaaggtagc gtttcatcta taactccttg aacttataac
    43561 catacccccg aatggttcga ataaattgag gggctttagg attgtcttca attttttccc
    43621 ttaacttacc aatatgaacg tccaccaaac gtgtttcctg cccaaagtca tacccccaga
    43681 tacgttccaa aagacgctct ctagtcagtg tcatgttggg atgtttcata agatagagca
    43741 agagttcaaa ttcttttggg gtcaaactca gtaacttatt cgccttgtag acttcatgac
    43801 gctcagggta tactttcaag gtcccaaata gccaagaatc gtcagcgata ttatctgaat
    43861 catctccttc ttgttctcct ttagttcgcc tgaggacagc cttgacacgc gccagcaatt
    43921 ctctagggct aaaaggcttg gtcaggtagt catcagcccc taattccaag gccaaaacct
    43981 tatcaaattc atcacttttc gcagaaacca tcataattgg agttttgacg cctttggctc
    44041 tcagccgctt acaaacttcc atgccatcta attgtggtaa catgatatca agcaagataa
    44101 aatcaaaggg ttctgtttct gccaaagcta aggccttccg tccatttgtt accaattgag
    44161 tagaaaagcc ttccttactt aaatggtagt caagcaattt cagaatgtgt tcttcatcat
    44221 ccactaataa gacttgtttt gtcatctatt atctcctatt ggtaacatta taacacaatt
    44281 atcagaaatc ctaacattgc taaatcagat taaatttgct tatcaagact agtatctggt
    44341 caaacgctca atcatctcct tgtgctctgg ataggtcgcc agtagatcta ccctttcaaa
    44401 taattcaaaa tcctcaaatt caaaaccagg agcaacaaga caagaaacca gagcatcatc
    44461 cttatcaact gttgatcccc aaatagtgcc cttaggaaca cagtagtgaa gttgttgccc
    44521 tttggatatg tccaggccta aagtgactgc ttcgtagtga ccatctgctg taatcatgtg
    44581 aacagtaagt ggggattctg catgaaaata ccagatttca tctgctgtca atcggtgaaa
    44641 atgtgaagga ttcgtttctt cttaataaga aataaatact ggtataaagc gcccttccct
    44701 taccagcaag gtttatagtg tctgaagctt tttttgtttg tctaaaatag ccaccttcaa
    44761 catggggagc taactctaga gttcttatca agtcttcttt atccgtcgga gccaatgggt
    44821 tgaagtaact cttgttcaaa gtggtgttac gatttcaaga actcctctca gttctgagga
    44881 cacggtaatg attgatgcga cggaagtaca aatcaatcgc cctaaaagaa gaattagcga
    44941 atgattctgg taaaaaaaat gccacgctat gaaggctcaa gcgattgtca caagtcaagg
    45001 gagaattgtt tctttggata tcgctgtgaa ctattgtcat gatatgaagt tgttcaaaat
    45061 gagtcgcaga aatatcggac aagctggtaa aatcttggct gacagtggtt atcaagggct
    45121 catgaagata tatcctcaag cacaaactcc acgtaaatcc agcaaactca agccactaac
    45181 agttgaagat aaagcctata accatgcgct atccaaggag agaagcaagg ttgagaacat
    45241 ctttgccaaa gtaaaaacgt ttaaaatgtt ttcaacaacc tatcgaaatc atcgtaaacg
    45301 cttcggatta cgaatgaatt tgattgctgg tattatcaat catgaactag gattctagtt
    45361 ttgcaggaag tctattattt ggttaggtga attagtgaag tgtttaggca agtgtctctg
    45421 gttacgacgt catggcctct aaatcgatta tatttagggg tcatgactag tgaagcagtt
    45481 agctagttcg catataagcg gctagcgtct aacaattagg aactttagtt ccaataactt
    45541 taagattacg acgttttagg acataaatcg atcatattta tgtcctaaaa ctagtgaagc
    45601 gcctagccaa agtccgaata ggatttggcg ttagttactt agattgcttt gcaatcaagt
    45661 aactttggcg atttacatct tctctggcgc ttctactcca agcaagcgaa gggcttcttt
    45721 gagaacgact gcggttgcgt agctgagggc tagacggctg tcgcgttctg ggctttcatc
    45781 caagatacgt gtatgtgcat agtatttgtt aaaggattga gccaggctaa ttgcaaattt
    45841 agcaatgata gaaggttcaa agttatctgc cgcacggttg ataatacgtg ggaagtcttg
    45901 aatgagttta atgatttccc agctttcagt atcattcaag ctatagttgc cagctgtttc
    45961 tggtttgaaa tcggctttgc gtaagataga ttggatacga gcgtaggcat attgaacgta
    46021 aggtccagtt tcaccctcga aggataccat agcttctagg tcgaagtcgt atccatttgt
    46081 acggtcggtt ttgaggtcat agaatttaat ggctccaacc ccaacagcat gtgctacttg
    46141 gtctttgttt tctagttcag gatttttagc ctcgatttgg accttggcac ggctaacagc
    46201 ctctgcaaca gtaggctcta gcaagatgac attcccttta cgagtagaga gtttcttccc
    46261 ttcttttgta accaaaccaa aaggaacgtg agtaatgtcg tcactccagt cgtagcccat
    46321 ctcttgcaag acagctttga gctgtttaaa gtgggcagat tgttcttgac caacgacata
    46381 gatagattta gcaaattggt attcgttttt acggtaaagg gctgcagcca agtcacgtgt
    46441 gatatagaga gttgcaccat cagatttctt gatgagggct ggatgttcaa ttccgtattt
    46501 ctcaagattg acaacttggg caccttctga ttcaagaagt agtccttttt cagaaagaat
    46561 gtctacaact gcatccatct tatcattgta gaaggcttct ccgttatagc tgtcaaattc
    46621 aaccttcaat tcattgtaaa ggcggttaaa ttccactaaa ctttcatcgc ggaaccattg
    46681 ccaaagagcg agagcttcct catctccatt ttcaagttta cggaaccatt cgcgcgcttc
    46741 ttcatccaag ctagggtcat tttcagcttc agcgttgatg cggacataga gtttaaggag
    46801 ttcatcgatt ggatgagctt ttacagcttc ttcgtcgccc cattttttgt aggcaacaat
    46861 caacatccca aattgtttac cccagtctcc caaatggttg accttgaccg tttgataacc
    46921 gattttttgg aaaatatgtg acaagctatc tccgataaca gttgaacgca ggtggccaat
    46981 agaaaatggt ttagcgatat tcggactaga catgtcgata acaacatttt cttgtttgcc
    47041 aatattttgg tcagcatagt gttctttttc agtggtaaca gcttgcaata cttgagcaga
    47101 aatggcagat ttatcaagga aaaagttaac gtaaggtcct gttgcgacaa ctttttcaaa
    47161 ggcttggctg ttcatttttt cagccagttc agccgcaatc atttgtggtg ctttacgttc
    47221 gacttttgca agagaaaaag cagggaaagc aatgtctccc atttctgagt ttttaggggt
    47281 ttccagtaac tttaaaatag cctcttggtc caggctatca atgatgctag ataattcgct
    47341 agcaatcaat tcttttgtat tcattaagag ctcctttttg gacttttcta ctattttatc
    47401 acaattttaa agaaagaaga aaaaattttt gaaatctcct gtttttttgg tataatatgg
    47461 ttataaatat agttataaat atgcacgcaa gaggatttta tgagaaaaag agatcgtcat
    47521 cagttaataa aaaaaatgat tactgaggag aaattaagta cacaaaaaga aattcaagat
    47581 cggttggagg cgcacaatgt ttgtgtgacg cagacaacct tgtctcgtga tttgcgcgaa
    47641 atcggcttga ccaaggtcaa gaaaaatgat atggtgtatt atgtactagt aaatgagaca
    47701 gaaaagattg atttggtgga atttttgtct catcatttag aaggtgttgc aagagcagag
    47761 tttaccttgg tgcttcatac caaattggga gaagcctctg ttttggcaaa tattgtagat
    47821 gtaaacaagg atgaatggat tttaggaaca gttgctggtg ccaatacctt attggttatt
    47881 tgtcgagatc agcacgttgc caaactcatg gaagatcgtt tgctagattt gatgaaagat
    47941 aagtaaggtc ttgggagttg ctctcaagac ttatttttga aaaggagaga cagaaaatgg
    48001 cgatagaaaa gctatcaccc ggcatgcaac agtatgtgga tattaaaaag caatatccag
    48061 atgctttttt gctctttcgg atgggtgatt tttatgaatt attttatgag gatgcggtca
    48121 atgctgcgca gattctggaa atttccttaa cgagtcgcaa caagaatgcc gacaatccga
    48181 tccctatggc gggtgttccc tatcattctg cccaacagta tatcgatgtc ttgattgagc
    48241 agggttataa ggtggctatc gcagagcaga tggaaaatcc taaacaagca gttggggttg
    48301 ttaaacgaga ggttgttcag gtcattacgc cagggacagt ggtcgatagc agtaagccgg
    48361 acagtcagaa taattttttg gttgccatag accgcgaagg caatcaattt ggcctagctt
    48421 atatggattt ggtgacgggt gacttttatg tgacaggtct tttggatttc acgctggttt
    48481 gtggggaaat ccgtaacctc aaggctcgag aagtggtgtt gggttatgac ttgtctgagg
    48541 aagaagaaca aatcctcagc cgccagatga atctggtact ctcttatgaa aaagaaagct
    48601 ttgaagacct tcatttattg gatttgcgat tggcaacggt ggagcaaacg gcatctagta
    48661 agctgctcca gtatgttcat cggactcaga tgagggaatt gaaccacctc aaacctgtta
    48721 tccgatacga aattaaggat ttcttgcaga tggattatgc gaccaaggct agtctggatt
    48781 tggttgagaa tgctcgctca ggtaagaaac aaggcagtct tttctggctt ttggatgaaa
    48841 ccaaaacggc tatggggatg cgtctcttgc gttcttggat tcatcgcccc ttgattgata
    48901 aggaacgaat cgtccaacgt caagaagtag tgcaggtctt tctcgaccat ttctttgagc
    48961 gtagtgactt gacagacagt ctcaagggtg tttatgacat tgagcgcttg gctagtcgtg
    49021 tttcttttgg caaaaccaat ccaaaggatc tcttgcagtt ggcgactacc ttgtctagtg
    49081 tgccacggat tcgtgcgatt ttagaaggga tggagcaacc tactctagcc tatctcatcg
    49141 cacaactgga tgcaatccct gagttggaga gtttgattag cgcagcgatt gctcctgaag
    49201 ctcctcatgt gattacagat gggggaatta tccggactgg atttgatgag actttagaca
    49261 agtatcgttg cgttctcaga gaagggacta gctggattgc tgagattgag gctaaggagc
    49321 gagaaaactc tggtatcagc acgctcaaga ttgactacaa taaaaaggat ggctactatt
    49381 ttcatgtgac caattcgcaa ctaggaaatg tgccagccca ctttttccgc aaggcgacgc
    49441 tgaaaaactc agaacgcttt ggaaccgaag aattagcccg tatcgaggga gatatgcttg
    49501 aggcgcgtga gaagtcagcc aacctcgaat acgaaatatt tatgcgcatt cgtgaagagg
    49561 tcggcaagta catccagcgt ttacaagctc tagcccaagg aattgcgacg gttgatgtct
    49621 tacagagtct ggcggttgtg gctgaaaccc agcatttgat tcgacctgag tttggtgacg
    49681 attcacaaat tgatatccgg aaagggcgcc atgctgtcgt tgaaaaggtt atgggggctc
    49741 agacctatat tccaaatacg attcagatgg cagaagatac cagtattcaa ctgattacag
    49801 ggccaaacat gagtgggaag tctacctata tgcgtcagtt agccatgacg gcggttatgg
    49861 cccagctggg ttcctatgtt cctgctgaaa gcgcccattt accgattttt gatgcgattt
    49921 ttacccgtat cggagcagca gatgacttgg tttcgggtca gtcgaccttt atggtggaga
    49981 tgatggaggc caataatgcc atttcgcatg cgaccaagaa ctctctcatt ctctttgatg
    50041 aattgggacg tggaactgca acttatgacg ggatggctct tgctcagtcc atcatcgaat
    50101 atatccatga gcacatcgga gctaagaccc tctttgcgac ccactaccat gagttgacta
    50161 gtctggagtc tagtttacaa cacttggtca atgtccacgt ggcaactctg gagcaggatg
    50221 ggcaggttac cttccttcac aagattgaac cgggaccagc tgataaatcc tacggtatcc
    50281 atgttgccaa gattgctggc ttgccagcag accttttagc aagggcggat aagattttga
    50341 ctcagctaga gaatcaagga acagagagtc ctcctcccat gagacaaact agtgctgtca
    50401 ctgaacagat ttcactcttt gatagggcag aagagcatcc tatcctagca gaattagcta
    50461 aactggatgt gtataatatg acacctatgc aggttatgaa tgtcttagta gagttaaaac
    50521 agaaactata aaaccaagac tcactagtta atctagctgt atcaaggaga cttctttgac
    50581 aattctccac ttttttgcta gaataacatc acacaaacag aatgaaaagg agctgacgca
    50641 ttgtcgctcc cttttgtcta ttttttaagg agaaagtatg ctgattcaga aaataaaaac
    50701 ctacaagtgg caggccctgg cttcgctcct gatgacaggc ttgatggttg ctagttcact
    50761 tctgcaaccg cgttatctgc aggaagtctt aggcgccctc cttactggga aatatgaagc
    50821 tatttatagt atcggggctt ggttgattgg tgtggccgta gtcggtctag ttgctggtgg
    50881 actcaatgtt gtcctcgcag cctatattgc ccaaggagtt tcatccgacc ttcgggagga
    50941 tgccttccgt aaaattcaaa ccttttctta tgctgatatt gaacaattta atgcgggaaa
    51001 tctagtcgtt cgaatgacaa atgatatcaa ccagattcag aacgttgtca tgatgacctt
    51061 ccaaattctt ttcagacttc ccctcttgtt catcggttcg tttatcctag cggttcaaac
    51121 cttaccttct ctgtggtggg tgattgttct catggtagtc ttgatttttg gtttgactgc
    51181 tgtcatgatg ggaatgatgg ggcctcgttt tgccaagttt caaacccttc ttgagcgcat
    51241 caatgccatt gccaaggaaa atttacgtgg cgttcgtgtg gtcaagtcct ttgtccaaga
    51301 aaaagagcaa tttgctaagt ttacagaggt ctcagacgag cttcttggtc aaaaccttta
    51361 cattggttat gccttttcag tagtggaacc ctttatgatg ttggttggtt acggggcggt
    51421 cttcctctct atttggctgg tcgcgggaat ggttcagtcg gatccgtctg ttgttggttc
    51481 catcgcttct tttgttaatt acctaagcca gattatcttt accattgtta tggttggatt
    51541 tttgggaaat tctgtcagcc gtgccatgat ttccatgcgt cgtattcgag aaattcttga
    51601 cgcagagcca gctatgacct tcaaggatat cccagatgaa gagttggttg gaagccttag
    51661 ctttgaaaat gtgaccttta cctatccaat ggacaaggaa ccgatgctga aagatgtgag
    51721 ctttactatt gaacctggtc aaatggttgg tgtagttgga gcgactggtg caggaaagtc
    51781 aaccttggct caattgattc cacgtctctt tgatccacag gaaggggcta tcaaaatcgg
    51841 aggtaaggat attcgagaag tgagtgaagg gaccctgcgt aaaacagttt ccatcgttct
    51901 ccaacgtgcc attcttttta gtggaacgat tgcagataac ttgagacagg ggaagggaaa
    51961 tgctactcta tttgaaatgg agcgcgcagc caatattgcc caggctagtg aattcattca
    52021 tcgtatggag aaaacctttg aaagtccagt tgaagaacgg ggaaccaatt tctctggtgg
    52081 acaaaaacaa aggatgtcga ttgcgcgtgg gattgtcagc aatccacgta ttctgatttt
    52141 tgatgattcg acctcagcct tggatgccaa atcagagcgc ttggtgcaag aagctttgaa
    52201 taaggacttg aaggggacga caactattat tattgctcaa aaaattagct cggttgtcca
    52261 tgcagacaag atcttggttc taaatcaagg acgattgatt ggtcaaggca cgcatgcaga
    52321 cttggttgcc aacaatgccg tttaccgtga aatctatgaa acacagaaat gaaagacaaa
    52381 ctataagaaa agtcaatagt tttatctaaa ctatttctta tttcaatttg atgatttggc
    52441 gatgatttta gagcacggca aaaagccctt gaaaaagtcc attttttcaa aggtaatcct
    52501 gtgttaattt cagaaattac atcacttttt gttcgtcaaa tggcagctct tttttaggat
    52561 ataaaacagg gttcggataa gtttttttgc aaggtggatg atggctacat tgtaatgttt
    52621 tccttgttct aacttagtct taagataggt cttaaaagca ggtgaaaagc gagggcatgc
    52681 tttggcagct tgtatgagta cctaccgcag atgaggggaa ccccgtttga ccattcttcc
    52741 agctaaatca atctgacctg actgataaat agaagaatcc agtccagcga aagcttgtaa
    52801 ttgagcagga ttatcaaagg catgaatatt tcgaatctcg gctaaaatga ccgcccctaa
    52861 acgattctca atcccagtaa ccgtcgtgat gaccgagttg aactcagcca tcaagtcatt
    52921 gacacatgtt tccgccttgt caatgagcct cttgtaatgt ttgatgtttt cattacacga
    52981 gataaaacgt ctatgcgtta tcaaactcat taccaattaa aacaaaaagc tgtggttaga
    53041 tcctttcgga aattgtcaag cgattggagg aaatgaacta atccacagcg gcttattcca
    53101 agtataccac ttgggctttg gcagtagcta actgcgctaa atataatata aggaggagta
    53161 aaatgaagac agttcaattt ttttggcatt attttaaggt ctacaagttc tcatttgtag
    53221 ttgtcatcct gatgattgtt ctggcgactt ttgcccaagc cctctttcca gtcttttctg
    53281 gacaagcggt gacgcagcta gccaatttag ttcaagctta tcaaaatggc aatccagaac
    53341 ttgtatggca aagcctatca ggaatcatgg tcaatcttgg cctgctggtt ttggttctat
    53401 ttatctctag tgtaatatac atgtgtctca tgacgcgcgt gattgcagaa tcgaccaacg
    53461 agatgcgcaa aggcctcttt ggtaagcttg ctcagttgac ggtttctttc tttgaccgtc
    53521 gacaagatgg cgatatcctg tctcatttta ccagtgattt ggataatatc ctccaagcct
    53581 ttaacgaaag cttgattcag gtcatgagca atattgtttt atacattggt ctgattcttg
    53641 tcatgttttc gagaaatgtg acgctggctc tcatcaccat tgccagcacc ccattggctt
    53701 tccttatgct gattttcatc gtgaaaatgg cacgcaaata caccaacctc cagcagaaag
    53761 aggtagggaa gctcaacgcc tatatggatg agagcatctc aggccaaaaa gccgtgattg
    53821 tgcaaggaat tcaagaggat atgatggcag gatttcttga acaaaatgag cgcgtgcgca
    53881 aggcaacctt taaaggaaga atgttctcag gaattctttt ccctgtcatg aatgggatga
    53941 gcctgattaa tacagccatc gtcatctttg ctggttcggc tgtacttttg aatgataagt
    54001 ctattgaaac aagtacagcc ctaggtttga ttgttatgtt tgcacaattt tcacagcagt
    54061 actaccagcc tattatccaa gttgcagcga gttggggaag ccttcagttg gcctttactg
    54121 gagctgaacg aattcaggaa atgtttgatg cagaggagga aatccgacct gaaaaggctc
    54181 caaccttcac taagttgcaa gaaagtgttg aaatcagtca tatcgatttt tcatacttgc
    54241 ctgataaacc tattttgaaa gatgtcagca tttctgcccc taaaggccag atgacagcag
    54301 ttgttgggcc gacaggttca ggaaaaacga ctattatgaa cctcatcaat cgcttttatg
    54361 atgttgatgc tggtggtatt tattttgatg gtaaagacat tcgtggctat gacttagata
    54421 gtcttagaag caaggtggga attgtattgc aagattcggt cttgtttagc ggaacgatta
    54481 gagacaatat ccgatttggt gtgccagatg ctagtcagga aatggttgag gtagcagcaa
    54541 aagcaaccca cattcacgac tatatcgaaa gtttgcctga taagtacgat actcttattg
    54601 atgatgacca gagcatcttt tcaacagggc agaagcaatt gatttcaatc gctcgaaccc
    54661 tgatgacaga tccagaagtt ctcattctcg atgaagcaac ttcaaacgta gatacggtga
    54721 cagaaagcaa gattcagcat gccatggagg tggttgtagc aggtagaact agtttcgtca
    54781 ttgcccaccg cttgaaaacc attctcaatg cagatcagat tattgtcctt aaagatggag
    54841 aagtcattga acgtggtaac caccatgaac ttttgaagct aggtggcttt tattcagaac
    54901 tctatcacaa tcaatttgtt ttcgaataag aaagaagttg tcctatgtgg gcagcttttt
    54961 cttgtccata aaaaatgttt atcacagcct taaaaaaaac atattagacg aaagtcattt
    55021 tgagtgatat gataggacta tcgttagcat tcgaaaggag aggcatcatg gctagaacgg
    55081 ttgtaggagt tgctgcaaat ctatgtcccg tagacgcaga aggcaaaatc attcattcat
    55141 ctgtatcttg tagattcgca gagatcattc gtcaagtcgg tggtctccct ttagtcattc
    55201 ctgttggtga tgagtcagtt gtacgtgatt atgtggaaat gattgacaaa ctcattttga
    55261 caggaggcca aaatgttcat cctcagtttt atggagagaa aaagaccgtc gaaagcgatg
    55321 attacaatct ggtccgtgac gaatttgaat tggcactctt gaaggaagcg cttcgtcaga
    55381 ataaaccaat tatggcaatc tgtcgcggtg tccaacttgt caatgttgcc tttggtggaa
    55441 ccctcaatca agaaatcgaa ggtcactggc aaggactacc tttcgggaca tctcactcta
    55501 ttgagacagt agaaggaagc gtggtggcta agctatttgg gaaagaaagt caggtcaact
    55561 cagtccatcg tcaaagcatt aaagatttgg cacctaattt ccgtgtaact gctattgatt
    55621 caagagacca aactattgaa gcgattgagt ctatcgacga gcaccgcatt atcggtttac
    55681 agtggcatcc agagtttctg gttaatgaag aagatggcaa tttagaatta tttgagtatt
    55741 tattgaatga actgtaacga ttggaacatc tggtcgttct ttcttttatt ttttcaaaat
    55801 ttttagaatg tggcatttta cgcaaacgtt tgaattctga taaaaattgg agaaattcga
    55861 caaaaaaact tgaaaaaaac gaaggtaagc gttatgatag aaaagaagaa atattggagg
    55921 aagaacatgt cacatattaa atttgattat tcaaaagttt tagacaaatt tgttgcacca
    55981 catgaagtgg aatacatgca atcacaagta acagcagcag atgaattgat ccgtaaagga
    56041 actggtgctg gtagcgactt cttgggatgg ttggaccttc ctgaaaaata cgaccgcgaa
    56101 gaatttgacc gcatcttgaa agctgctgag caaatcaagt cagacagcga tgttttggtt
    56161 gtgatcggta ttggtggatc ttaccttggt gcaaaagcag caatcgactt cttgaatcac
    56221 cattttgcta acttgcaaac aaaagaagaa cgcaaagctc cacaaatcct ttacgcagga
    56281 aactcaatct catctactta ccttgctgac ttggtagagt atgttgcaga caaggacttc
    56341 tcagtaaacg taatttctaa atcaggtaca acaactgaac cagcgattgc tttccgtgtc
    56401 tttaaagaac tcttggttaa gaaatacggt caagaagaag ctaacaaacg tatctatgca
    56461 acaactgacc gccaaaaggg tgctgttaag gttgaagcag acgctaacgg ttggggaaca
    56521 tttgttgttc cagatgatat cggcggacgc ttctcagtat tgacagccgt tggtttgctt
    56581 ccaatcgcag catcaggagc tgacatcaaa gctcttatgg aaggtgcgaa tgcagctcgc
    56641 aaagactaca cttcagacaa aatctctgaa aacgaagctt accaatacgc agccgttcgt
    56701 aacatccttt atcgtaaagg ctatgcaact gagatcttgg taaactatga gccatcactt
    56761 caatacttct cagaatggtg gaaacaattg gctggtgaat cagaaggaaa agaccaaaaa
    56821 ggtatctacc caacttcagc caacttctca actgacttgc actcacttgg tcaatttatc
    56881 caagaaggaa ctcgtatcat gtttgaaaca gttgtccgtg ttgacaaacc tcgtaaaaac
    56941 gtgcttattc ctactttgga agaagacctt gacggacttg gttaccttca aggaaaagac
    57001 gttgactttg taaacaaaaa agcaactgac ggtgttcttc ttgcccacac agatggtgat
    57061 gtaccaaaca tgtatgtgac tcttccagag caagacgctt tcactcttgg ttacactatc
    57121 tacttcttcg aattggcaat tgccctttca ggttacttga atgctatcaa cccatttgac
    57181 caaccaggtg ttgaagctta taaacgtaac atgtttgccc ttcttggaaa accaggattt
    57241 gaagaattga gcaaagaact taacgcacgt ctataataga agaaaagagt ggtttgccca
    57301 ctctttttac tctctttatc catagaaatt ggactcagcc aagacttgtg atataatata
    57361 gaaagcaaaa aggcagacgc ctagataata ggagaaacta tgtcaaaaga tatccgcgta
    57421 cgttacgcac caagtccaac aggactacta cacatcggaa atgctcgtac agcattgttt
    57481 aattacttgt atgcgcgcca tcatggtgga acatttctca tccgtatcga agatactgac
    57541 cgtaaacgcc atgtcgagga tggtgaacgt tcacaacttg aaaaccttcg ctggttaggc
    57601 atggattggg atgaaagtcc agaatcatat gagaattatc gccagtctga gcgtttagac
    57661 ttgtatcaaa aatacattga ccaactatta gctgaaggaa aagcctataa atcttacgtt
    57721 acagaagaag agttggcagc tgaacgcgaa cgccaagaag tagctggcga aacaccacgc
    57781 tacatcaatg aataccttgg tatgagtgaa gaagaaaaag cagcttacat cgcagaacgt
    57841 gaagcagcag ggatcatccc aactgttcgt ttggctgtca atgagtcagg tatctacaag
    57901 tggcatgata tggtcaaagg cgatatcgaa tttgaaggtg gcaatatcgg tggtgactgg
    57961 attatccaaa agaaagacgg ttacccaact tacaactttg ccgttgccat cgatgaccat
    58021 gatatgcaaa tctcccatgt tatccgtgga gacgaccata ttgctaacac accaaaacag
    58081 ctcatggttt atgaagcact tggttgggaa gctccagagt ttggtcacat gaccttgatt
    58141 atcaactctg agactggtaa gaagttatct aaacgtgata ctaatacact tcaatttatc
    58201 gaagattacc gaaagaaagg ttaccttcca gaagcagtct ttaattttat tgctcttctt
    58261 ggttggaacc caggtggtga agatgagatt ttctctcgtg aagaactcat taaacttttt
    58321 gatgaaaacc gcctcagcaa gtcaccagca gcctttgatc agaaaaaatt agactggatg
    58381 agcaatgatt atatcaagaa tgcagacctt gaaaccatct ttgaaatggc aaaaccattc
    58441 ttagaggaag caggccgttt gactgacaag gctgaaaaat tagttgagct ctataaacca
    58501 caaatgaaat cagtagatga gattatccca ttgacagatc ttttcttctc agatttccca
    58561 gaattgacag aagcagagcg cgaagtcatg acgggtgaaa cagttccaac agttcttgaa
    58621 gcattcaaag caaaacttga agcgatgaca gatgatgaat ttgtgacaga aaatatcttc
    58681 ccacaaatta aagcagtcca aaaagaaaca ggtattaaag ggaaaaatct tttcatgcct
    58741 attcgtatcg cagtttcagg cgaaatgcat gggccagaat taccagatac aattttcttg
    58801 cttggacgtg aaaaatcaat tcagcatatc gaaaacattc taaaagaaat ctctaaataa
    58861 agaggataca ataatggaca tctgggaaaa gatgtacgaa gaagcacaga aactatacaa
    58921 tccacatgaa gtatcagact ttgtttatgt caatcatgtt gtagctgcag tagaagcagg
    58981 agatggacaa atatttacag gattctgtat ggagggaacc tgtggtgttt tccatctctg
    59041 cgcagaacgg gcggccctct tcaatatgta ccaattttca ggacaaacta aggttaagaa
    59101 agtattagcc tttcgagata aaccacctta tggtggaagt tcagccatgc cttgcggtgc
    59161 ttgtagagaa ttccttttag agcttaatgc tgaaaataaa gatgcagaat tcatgatgga
    59221 ctataatata agaaaaacag ttaaagtctc agaactaatc ccttattggt ggggagaaga
    59281 acgtgcttct aagtttaatg agcgatagaa gagtcagtat aattctggct ctttttacta
    59341 taaattgaaa aaataaaaaa aatta
//
LOCUS       DAVLYV010000004        58888 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLYV010000004 DAVLYV010000000
VERSION     DAVLYV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58888
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.004"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            284..940
                     /locus_tag="VYO70_000202"
     CDS             284..940
                     /locus_tag="VYO70_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1712498.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            930..1253
                     /locus_tag="VYO70_000203"
     CDS             930..1253
                     /locus_tag="VYO70_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEV1712499.1"
                     /translation="MVSKYLLLAVIFSGLVTWIPRMILFILVKHKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1357..2187
                     /locus_tag="VYO70_000204"
     CDS             1357..2187
                     /locus_tag="VYO70_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1712500.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2341..3195
                     /locus_tag="VYO70_000205"
     CDS             2341..3195
                     /locus_tag="VYO70_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1712501.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3295..4668
                     /locus_tag="VYO70_000206"
     CDS             3295..4668
                     /locus_tag="VYO70_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1712502.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4661..5722
                     /locus_tag="VYO70_000207"
     CDS             4661..5722
                     /locus_tag="VYO70_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1712503.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5724..6416
                     /locus_tag="VYO70_000208"
     CDS             5724..6416
                     /locus_tag="VYO70_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712504.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6446..7015)
                     /locus_tag="VYO70_000209"
     CDS             complement(6446..7015)
                     /locus_tag="VYO70_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1712505.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7150..7764
                     /locus_tag="VYO70_000210"
     CDS             7150..7764
                     /locus_tag="VYO70_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1712506.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLVGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKILLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7766..9412
                     /locus_tag="VYO70_000211"
     CDS             7766..9412
                     /locus_tag="VYO70_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1712507.1"
                     /translation="MKHFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9424..10710
                     /locus_tag="VYO70_000212"
     CDS             9424..10710
                     /locus_tag="VYO70_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1712508.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10762..11295
                     /locus_tag="VYO70_000213"
     CDS             10762..11295
                     /locus_tag="VYO70_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712509.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELA"
     gene            11373..11843
                     /gene="nrdI"
                     /locus_tag="VYO70_000214"
     CDS             11373..11843
                     /gene="nrdI"
                     /locus_tag="VYO70_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1712510.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12136..13398)
                     /locus_tag="VYO70_000215"
     CDS             complement(12136..13398)
                     /locus_tag="VYO70_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1712511.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDAVFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13708..14166)
                     /locus_tag="VYO70_000216"
     CDS             complement(13708..14166)
                     /locus_tag="VYO70_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1712512.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(14163..14603)
                     /locus_tag="VYO70_000217"
     CDS             complement(14163..14603)
                     /locus_tag="VYO70_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1712513.1"
                     /translation="MKVELQISETYEEEKLIVQAPQETEKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14959..16908
                     /gene="mutL"
                     /locus_tag="VYO70_000218"
     CDS             14959..16908
                     /gene="mutL"
                     /locus_tag="VYO70_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1712514.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(17233..17700)
                     /locus_tag="VYO70_000219"
     CDS             complement(17233..17700)
                     /locus_tag="VYO70_000219"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1712515.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17701..18906)
                     /locus_tag="VYO70_000220"
     CDS             complement(17701..18906)
                     /locus_tag="VYO70_000220"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1712516.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERSGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18926..19561)
                     /gene="ribE"
                     /locus_tag="VYO70_000221"
     CDS             complement(18926..19561)
                     /gene="ribE"
                     /locus_tag="VYO70_000221"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1712517.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19546..20646)
                     /gene="ribD"
                     /locus_tag="VYO70_000222"
     CDS             complement(19546..20646)
                     /gene="ribD"
                     /locus_tag="VYO70_000222"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1712518.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVQQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20780..20894)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            21051..21644
                     /gene="ruvA"
                     /locus_tag="VYO70_000223"
     CDS             21051..21644
                     /gene="ruvA"
                     /locus_tag="VYO70_000223"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1712519.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21654..22217
                     /locus_tag="VYO70_000224"
     CDS             21654..22217
                     /locus_tag="VYO70_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1712520.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQVLFELLCMETYQA
                     GLSWETVLNKRQAFREVFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKLFATRANAQ
                     VFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            22214..22891
                     /locus_tag="VYO70_000225"
     CDS             22214..22891
                     /locus_tag="VYO70_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1712521.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(23046..24077)
                     /locus_tag="VYO70_000226"
     CDS             complement(23046..24077)
                     /locus_tag="VYO70_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1712522.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCPKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(24332..25015)
                     /locus_tag="VYO70_000227"
     CDS             complement(24332..25015)
                     /locus_tag="VYO70_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1712523.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(25027..25971)
                     /locus_tag="VYO70_000228"
     CDS             complement(25027..25971)
                     /locus_tag="VYO70_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1712524.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            26103..28934
                     /gene="uvrA"
                     /locus_tag="VYO70_000229"
     CDS             26103..28934
                     /gene="uvrA"
                     /locus_tag="VYO70_000229"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1712525.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKADVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            28927..29988
                     /locus_tag="VYO70_000230"
     CDS             28927..29988
                     /locus_tag="VYO70_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1712526.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASSLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            30120..30518
                     /gene="spx"
                     /locus_tag="VYO70_000231"
     CDS             30120..30518
                     /gene="spx"
                     /locus_tag="VYO70_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1712527.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            30584..31153
                     /locus_tag="VYO70_000232"
     CDS             30584..31153
                     /locus_tag="VYO70_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1712528.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGTATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKIYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            31240..31506
                     /locus_tag="VYO70_000233"
     CDS             31240..31506
                     /locus_tag="VYO70_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1712529.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            31510..31929
                     /gene="ruvX"
                     /locus_tag="VYO70_000234"
     CDS             31510..31929
                     /gene="ruvX"
                     /locus_tag="VYO70_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1712530.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            31945..32250
                     /locus_tag="VYO70_000235"
     CDS             31945..32250
                     /locus_tag="VYO70_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1712531.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            32505..33755
                     /locus_tag="VYO70_000236"
     CDS             32505..33755
                     /locus_tag="VYO70_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1712532.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            33752..34297
                     /locus_tag="VYO70_000237"
     CDS             33752..34297
                     /locus_tag="VYO70_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1712533.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            34443..35975
                     /gene="cls"
                     /locus_tag="VYO70_000238"
     CDS             34443..35975
                     /gene="cls"
                     /locus_tag="VYO70_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1712534.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            36233..37771
                     /locus_tag="VYO70_000239"
     CDS             36233..37771
                     /locus_tag="VYO70_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1712535.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            37891..40098
                     /gene="nrdD"
                     /locus_tag="VYO70_000240"
     CDS             37891..40098
                     /gene="nrdD"
                     /locus_tag="VYO70_000240"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1712536.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            40110..40250
                     /locus_tag="VYO70_000241"
     CDS             40110..40250
                     /locus_tag="VYO70_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712537.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKYSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            40294..40800
                     /locus_tag="VYO70_000242"
     CDS             40294..40800
                     /locus_tag="VYO70_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1712538.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            40793..41383
                     /gene="nrdG"
                     /locus_tag="VYO70_000243"
     CDS             40793..41383
                     /gene="nrdG"
                     /locus_tag="VYO70_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1712539.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELSDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            41380..41997
                     /locus_tag="VYO70_000244"
     CDS             41380..41997
                     /locus_tag="VYO70_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1712540.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTLAQELSQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAYELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRFARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            42252..42560
                     /gene="rpsJ"
                     /locus_tag="VYO70_000245"
     CDS             42252..42560
                     /gene="rpsJ"
                     /locus_tag="VYO70_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1712541.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            42776..43402
                     /gene="rplC"
                     /locus_tag="VYO70_000246"
     CDS             42776..43402
                     /gene="rplC"
                     /locus_tag="VYO70_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1712542.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            43427..44050
                     /gene="rplD"
                     /locus_tag="VYO70_000247"
     CDS             43427..44050
                     /gene="rplD"
                     /locus_tag="VYO70_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1712543.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            44050..44346
                     /locus_tag="VYO70_000248"
     CDS             44050..44346
                     /locus_tag="VYO70_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1712544.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            44364..45197
                     /gene="rplB"
                     /locus_tag="VYO70_000249"
     CDS             44364..45197
                     /gene="rplB"
                     /locus_tag="VYO70_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1712545.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            45301..45582
                     /gene="rpsS"
                     /locus_tag="VYO70_000250"
     CDS             45301..45582
                     /gene="rpsS"
                     /locus_tag="VYO70_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1712546.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            45594..45938
                     /gene="rplV"
                     /locus_tag="VYO70_000251"
     CDS             45594..45938
                     /gene="rplV"
                     /locus_tag="VYO70_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1712547.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            45951..46604
                     /gene="rpsC"
                     /locus_tag="VYO70_000252"
     CDS             45951..46604
                     /gene="rpsC"
                     /locus_tag="VYO70_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1712548.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            46608..47021
                     /gene="rplP"
                     /locus_tag="VYO70_000253"
     CDS             46608..47021
                     /gene="rplP"
                     /locus_tag="VYO70_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1712549.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            47031..47237
                     /locus_tag="VYO70_000254"
     CDS             47031..47237
                     /locus_tag="VYO70_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1712550.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            47262..47522
                     /gene="rpsQ"
                     /locus_tag="VYO70_000255"
     CDS             47262..47522
                     /gene="rpsQ"
                     /locus_tag="VYO70_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1712551.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            47548..47916
                     /gene="rplN"
                     /locus_tag="VYO70_000256"
     CDS             47548..47916
                     /gene="rplN"
                     /locus_tag="VYO70_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1712552.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            47994..48299
                     /gene="rplX"
                     /locus_tag="VYO70_000257"
     CDS             47994..48299
                     /gene="rplX"
                     /locus_tag="VYO70_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1712553.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            48323..48865
                     /gene="rplE"
                     /locus_tag="VYO70_000258"
     CDS             48323..48865
                     /gene="rplE"
                     /locus_tag="VYO70_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1712554.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            48883..49152
                     /gene="rpsN"
                     /locus_tag="VYO70_000259"
     CDS             48883..49152
                     /gene="rpsN"
                     /locus_tag="VYO70_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1712555.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            49437..49835
                     /gene="rpsH"
                     /locus_tag="VYO70_000260"
     CDS             49437..49835
                     /gene="rpsH"
                     /locus_tag="VYO70_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1712556.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGSNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            50027..50563
                     /gene="rplF"
                     /locus_tag="VYO70_000261"
     CDS             50027..50563
                     /gene="rplF"
                     /locus_tag="VYO70_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1712557.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            50647..51003
                     /gene="rplR"
                     /locus_tag="VYO70_000262"
     CDS             50647..51003
                     /gene="rplR"
                     /locus_tag="VYO70_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1712558.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            51021..51515
                     /gene="rpsE"
                     /locus_tag="VYO70_000263"
     CDS             51021..51515
                     /gene="rpsE"
                     /locus_tag="VYO70_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1712559.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            51487..51711
                     /gene="rpmD"
                     /locus_tag="VYO70_000264"
     CDS             51487..51711
                     /gene="rpmD"
                     /locus_tag="VYO70_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1712560.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            51856..52296
                     /gene="rplO"
                     /locus_tag="VYO70_000265"
     CDS             51856..52296
                     /gene="rplO"
                     /locus_tag="VYO70_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1712561.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            52309..53619
                     /gene="secY"
                     /locus_tag="VYO70_000266"
     CDS             52309..53619
                     /gene="secY"
                     /locus_tag="VYO70_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1712562.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            53770..54408
                     /locus_tag="VYO70_000267"
     CDS             53770..54408
                     /locus_tag="VYO70_000267"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1712563.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            54525..54743
                     /gene="infA"
                     /locus_tag="VYO70_000268"
     CDS             54525..54743
                     /gene="infA"
                     /locus_tag="VYO70_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1712564.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            54768..54884
                     /gene="rpmJ"
                     /locus_tag="VYO70_000269"
     CDS             54768..54884
                     /gene="rpmJ"
                     /locus_tag="VYO70_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1712565.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            54902..55267
                     /gene="rpsM"
                     /locus_tag="VYO70_000270"
     CDS             54902..55267
                     /gene="rpsM"
                     /locus_tag="VYO70_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1712566.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            55285..55668
                     /gene="rpsK"
                     /locus_tag="VYO70_000271"
     CDS             55285..55668
                     /gene="rpsK"
                     /locus_tag="VYO70_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1712567.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            55711..56646
                     /locus_tag="VYO70_000272"
     CDS             55711..56646
                     /locus_tag="VYO70_000272"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1712568.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            56658..57044
                     /gene="rplQ"
                     /locus_tag="VYO70_000273"
     CDS             56658..57044
                     /gene="rplQ"
                     /locus_tag="VYO70_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1712569.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            57309..57575
                     /locus_tag="VYO70_000274"
     CDS             57309..57575
                     /locus_tag="VYO70_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1712570.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            57585..>58888
                     /locus_tag="VYO70_000275"
     CDS             57585..>58888
                     /locus_tag="VYO70_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1712571.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPNINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGG"
BASE COUNT        17566 a        11167 c        13091 g        17064 t
ORIGIN      
        1 tttgttctca tcttatttca atctgctata taatagtcca tgctgtggct ggattctttt
       61 cagcctactt atactcttcg aaactctctt caaaccacgt cagcttcacc ttgccgtata
      121 tgtgttactg acttcgtcag ttctatccac aacctcaaaa cggtgttttg agcaacctgc
      181 ggctagtttc ctagtttgct ctttgatttt cattgagtat tattaaaaca atatgaggag
      241 aaagatgaaa gaaaaaggat tttaggaggg ggcgcaggca gccatgccaa cggctcttgg
      301 ctatgtcagt atcggcctgg cctgtgggac tatcggtgct ccctatgtga cacctgttga
      361 gatgggcttg atgagtctct ttgtttatgc tgggagtgcc cagtttgcca tgttggcact
      421 gattgtggtt caagctcctg tggcagctat tgctatgacg gtttttctaa tcaacttgcg
      481 tctctttttg ttgagtttac acgcatcgac ttatttccgt cataccagtc tttggtacaa
      541 tatcggtatg tctagtatct tgacagatga gacctatggc gttttgatgg gtgaattggc
      601 ccatacagac aaggtaaatc ctatgtggat gcacgggaac aatctcaaca gctatgtggc
      661 ttggtttgtg gggacagtag tcggaacggc tctgggtggc ctgctaccaa atccagaaat
      721 ctttggcttg gattttgccc tggttgggat gtttattggt atttttgctt cgcaatttca
      781 gattatgcaa agacggattc ctgtccgcaa tctgctcatt atcctagcag ttgttgcggt
      841 gtccttcttt ttgctcttga cagtggtgtc tcagtcacta gctgttctgt ttgcgacgct
      901 acttggttgt agcatggggg tggttttaga tggtcagtaa gtatctttta ttagcagtta
      961 ttttctctgg tcttgtgacc tggattcccc gtatgattct cttcatcttg gtcaagcata
     1021 agggcctgcc tgcgattgtt gagcgttttt tgaagttttt gcctgtttcc attatctttg
     1081 ccttgattct ttcaagcgta gtgacaggta aggttgggag ccttcctcaa attaaatggc
     1141 tagacttctt agccgtcttt ccaacagctt gggtagcctt tcgctaccgc aatctagtcg
     1201 gaacagttct ctttggagtg gtcttgattg ccatcttgcg tttggtcttt taaattaccc
     1261 ataaaaaaaa cttatcacag agatagatat catataatgg cgtaaatgct ctttttctgt
     1321 taagattata aggtattcta ttttggagga aatgacatga aaaaaatcgt taaatactca
     1381 tctcttgcag cccttgctct tgttgctgca ggtgtgcttg cggcttgctc aggtggtgct
     1441 aagaaagaag gacaagcagc tagcaagaaa gaaatcatcg ttgcaaccaa tggatcacca
     1501 aggccattta tctatgaaga aaatggcgaa ttgactggtt acgagattga agtcgttcgc
     1561 gctatcttta aagattctga caaatatgat gtcaagtttg aaaagacaga atggtcaggt
     1621 gtctttgctg gtcttgacgc tgaccgttac aatatggctg tcaacaatct tagctacact
     1681 aaagaacgtg cggagaaata cctctatgcc gcaccaattg cccaaaatcc taatgtcctt
     1741 gtcgtgaaga aagatgactc tagtatcaag tctctcgatg atatcggtgg aaaatcgacg
     1801 gaagtcgttc aagccactac atcagctaag cagttagaag catacaacgc tgaacacacg
     1861 gacaacccaa ctatccttaa ctatactaag gcagatttcc aacaaatcat ggtacgtttg
     1921 agcgatggac aatttgacta taagattttt gataaaatcg gtgttgaaac agtgatcaag
     1981 aaccaaggtt tggacaactt gaaagttatc gaacttccaa gcgaccaaca accgtacgtt
     2041 tacccacttc ttgctcaggg tcaagatgag ttgaaatcgt ttgtagacaa acgcatcaaa
     2101 gaactttata aagatggaac tcttgaaaaa ttgtctaaac aattcttcgg agacacttat
     2161 ctaccggcag aagctgatat taaataattt cttgaaatca tccattctga gtaagatggg
     2221 tggtttctca gtttttaggg atatagtttt aaactatata tctgactatc taataacaat
     2281 ttgtgaaatc aaacaaattg tgagatacta gtacggtatt atttttaagg agaaagaatc
     2341 atgaaaatca aaaaatggct tggtctagca gcccttgcta cagtcgcagg tttggctctt
     2401 gcagcttgcg gaaactcaga aaagaaagca gacaatgcaa caactatcaa aatcgcaact
     2461 gttaaccgta gcggttctga agaaaaacgt tgggacaaaa tccaagaatt ggttaaaaaa
     2521 gacggaatta ccttggaatt tacagagttc acagactact cacaaccaaa caaagcaact
     2581 gctgatggcg aagtagattt gaacgctttc caacactata acttcttgaa caactggaac
     2641 aaagaaaacg gaaaagacct tgtagcgatt gcagatactt acatctctcc aatccgcctt
     2701 tactcaggtt tgaatggaag tgccaacaag tacactaaag tagaagacat cccagcaaac
     2761 ggagaaatcg ctgtaccgaa tgacgctaca aacgaaagcc gtgcgcttta cttgcttcaa
     2821 tcagctggct tgattaaatt ggatgtttct ggaactgctc ttgcaacagt tgccaacatc
     2881 aaagaaaatc caaagaactt gaaaatcact gaattggacg ctagccaaac agctcgttca
     2941 ttgtcatcag ttgacgctgc cgttgtaaac aataccttcg ttacagaagc aaaattggac
     3001 tacaagaaag cacttttcaa agaacaagct gatgaaaact caaaacaatg gtacaacatc
     3061 attgttgcga aaaaagattg ggaaacatca cctaaggctg atgctatcaa gaaagtaatc
     3121 gcagcttacc acacagatga cgtgaaaaaa gttatcgaag aatcatcaga cggtttggat
     3181 caaccagttt ggtaataaga aacagggagg tgggagagag aattccacct cttgcttttg
     3241 tatagagcac ggagtgtaaa gaagactatt cgttcataga aaggtagaga gaatatggtt
     3301 tttcctagcg aacaagaaca gattgaaaaa tttgaaaagg atcatgtagc ccagcattat
     3361 tttgaggttt tgcgtacctt gatttctaag aaatcagtct ttgcccagca ggttggactc
     3421 aaggaagtcg caaattatct gggtgagatt ttcaagcgtg ttggagctga agtggagatt
     3481 gatgagagct atacagcgcc ctttgtcatg gcacatttca agagttcgcg tccagatgcc
     3541 aagaccttga ttttctataa ccactatgac actgtgccag cggatgggga tcaggtctgg
     3601 acagaggatc cttttacgct ttcggtccgc aatggcttca tgtatgggcg tggagttgat
     3661 gacgacaagg gtcatatcac agctcgcttg agtgctttga gaaaatatat gcagcaccat
     3721 gatgatttac ctgtcaatat cagctttatc atggagggag cggaggaatc ggcttcaaca
     3781 gacctagata agtatttgga aaagcatgca gacaaactcc gtggggcgga tttgttggtc
     3841 tgggaacaag ggaccaaaaa tgccttggaa cagctggaaa tttctggtgg caataagggg
     3901 attgtgacct ttgatgccaa ggtaaaaagc gctgatgtgg atatccactc gagttatggt
     3961 ggtgttgtgg aatcagctcc ttggtatctc ctccaagcct tacagtctct tcgtgctgcg
     4021 gatggccgta tcttggttga aggcttgtac gaagaagtac aagagcccaa tgaacgagaa
     4081 atggccttgc tagaaactta tggtcaacga aacccagagg aagttagtcg gatttatgga
     4141 ttggagttgc ctctcttaca ggaggagcgg atggcctttc taaaacgttt ctttttcgag
     4201 ccagcgctta atatcgaagg aatccagtct ggttatcaag gtcagggtgt gaagactatt
     4261 ttacctgcag aagccagtgc caagctagag gttcgtctgg ttccgggcct agaaccgcat
     4321 gatgttctgg aaaaaattcg gaaacagcta gacaaaaatg gctttgataa ggtagaatta
     4381 tactatacct tgggagagat gagctatcga agcgatatga gcgcaccagc cattctcaat
     4441 gtgatcgagt tggccaagaa attctatcca cagggcgttt cagtcttgcc gacgacagcg
     4501 gggacaggac ctatgcatac ggtctttgat gccctagagg taccaatggt tgcattcggt
     4561 ctaggaaatg ccaatagccg agaccacggt ggagatgaaa atgtgcgaat cgctgattat
     4621 tacacccata tcgaattagt agaggagctg attagaagct atgagtagag atattatcaa
     4681 gttagatcag atcgatgtga cttttcacca aaagaagaga accatcacag cggttaagga
     4741 tgtgaccatt cacatccaag aaggggatat ctacggaatc gttggatatt ctggagcagg
     4801 gaaatcaacc cttgtacggg tgattaatct cttgcaaaaa ccatctgcag ggaaaattac
     4861 cattgacgac gatgtgattt ttgatggtaa ggtcaccttg acggcagagc agttgcgtcg
     4921 taaacgtcaa gatatcggga tgattttcca gcattttaac ctgatgagtc aaaagacagc
     4981 agaggagaat gtagcctttg cccttaaaca ctctggactc agcaaggaag aaaagaaggc
     5041 taaagtagct aagttgttgg acttggttgg tttggcagat cgtgctgaaa actacccttc
     5101 acaattatct ggagggcaaa aacagcgtgt ggcaattgcg cgtgccttgg ccaatgatcc
     5161 aaaaatcttg atttcagacg agtcaacttc tgcccttgac cctaagacaa ccaagcagat
     5221 tttggccttg ttgcaagatt tgaaccaaaa attaggcttg actgttgtct tgattacgca
     5281 tgaaatgcag attgtcaaag acattgccaa ccgtgttgca gttatgcagg atgggcattt
     5341 gattgaagag ggtagtgtgc ttgaaatctt ctcaaaccct aaacaacctt tgactcaaga
     5401 ctttatctca acagctacag gtattgacga agccatggtc aaaatcgaga agcaagaaat
     5461 cgtggaacac ttgtctgaaa acagtctctt ggtgcaactc aagtacgctg gagcttcaac
     5521 agacgagcca cttttgaatg aattgtacaa gcattaccaa gtaatggcta atattctcta
     5581 tgggaatatc gaaattctcg atggtactcc tgttggagaa ttggtggtgg ttttgtcagg
     5641 tgaaaaagca gcgttggcag gtgcccaaga agccattcgt caagcaggtg tacaactaaa
     5701 agtattgaag ggagtacagt aagatggaat cattgattca aacctattta ccaaatgtct
     5761 ataaaatggg ttgggctggt caggcaggct ggggaacggc tatctactta actctttata
     5821 tgacagttct ttccttcatt atcggaggct tcttggggct agtggcaggt ctctttctcg
     5881 tcttgacagc gccaggtggt gtcttggaga ataaagtcgt attctggatt ttagacaaaa
     5941 ttacctcaat ttttcgtgcg gttcccttta tcatcctctt ggcaatcttg tcaccacttt
     6001 ctcacttgat tgttaaaaca agtatcgggc caaatgcagc ccttgtccca ctttcttttg
     6061 cagtctttgc cttctttgcc cgtcaggtgc aggttgtctt ggctgaattg gatggcggtg
     6121 tcattgaggc ggctcaagcg agcggagcga ctttctggga catcgtgggt gtttacctat
     6181 cagaaggtct tccagatttg atccgtgtga cgactgtgac cttgatttcc cttgttgggg
     6241 aaacagctat ggccggtgcg gttggagctg gtggtatcgg taacgtagcc atcgcttatg
     6301 gatttaaccg ctacaatcac gatgtgacca tcttggcaac catcattatc attttgatta
     6361 tctttgcaat ccaattcttg ggagatttct tgactaagaa attgagccat aaataaaaaa
     6421 gagccgcgtg gctcttttta actgatcaga ttttctgtgc aaatttttta ctcaaggctt
     6481 gtccaatcaa ggcacccact agggctccga tgacaatact tgcgataaat agaaggacag
     6541 ttccagggtt tggagcgacc atgatgcggt cgatatattc ttgggatttt cctcttgcca
     6601 gaagagtagc catataggct ttgggcgcaa tccacataag caagattggt cctgttgtac
     6661 taaaggcgaa aataatgaaa gaaaggaagt tctttgtttt gtccttgtat tttcctaaat
     6721 gagctacttc atctgctagg aggccacaga taattccagg aaggaaggaa ccagcaccgt
     6781 gtttagttcc caagaaaaag agggcaatga caaggccgat agtggtaatg gctccaaagc
     6841 gcggaacttt tgcgactagg atcatataga cgctaccgcc gacaagggca gtaaaggcag
     6901 gcgcataaaa catgtttcca gtttggtcaa agagattgcc caaaaggaca ccaatcccca
     6961 tgcagaggaa gtaaagaact gcaaaaagca gtgtagttaa gatagatttc ttcatgaatt
     7021 gtctcctgat aattttttca caattctcat tgtaccacgg tttggtggga ttgtaaatgg
     7081 atactagaag tttttgaaaa cgcttgaaat atgatataat ggtttcatag ttatttttag
     7141 gggaatatca tgggtagatt tttagacttt gtctttaatc gtttcttttt agggatgatt
     7201 gcgacagcct tcttttggct attaacttta gtaggaggga ttatccttgg tctagcgcca
     7261 gctagtgcca ccttgatgag cttatatgca gaacatggtt atagctttcg ggaatacagt
     7321 ttgaaggagg cttggtctct ttacaagcaa aattttgtct caagcaacct gattttctat
     7381 agctttttag gtgtgggtct agttttgacc tatggtttgt atctcttggt gcaattgcct
     7441 catcagacca ttgttcattt gattgcgacc cttttgaatg tcctagtagt tgccctgatc
     7501 tttttggctt atacagtatc tttaaaatta caagtttatt ttgccttgtc ctatcgaaat
     7561 agtctcaaat tatccttgat tggcatcttt atgagtctag cagctgtggc caagattctc
     7621 cttgggactg tgctacttgt agcaattggt tactatatgc ctgccctgct attttttgta
     7681 ggaattggga tgtggcattt ctttatcagt gatatgttgg aacctgtcta tgaaatcatc
     7741 catgaaaaat tggcgacaaa atagaatgaa gcacttttgg ctacatacgc ttctaagaac
     7801 ctatagttca gtgatgatca ttatcattgc gagttttgca atcttactct cttacgctga
     7861 ctgggattca cgtgaaaagg aagcccagag agtagcccag cgtgtaactg ctagaacagt
     7921 gagtgaaatt gaatattacc atagagagtc aacccagata gctcaggctt tagttgaaaa
     7981 ccaagctcgt attgagggaa tctataaata ctttagcctt agcatgccag actattttta
     8041 ctggcaatta gagcggaaag cttcgcctta tatatcagtc tctctgtatg aaaatgttga
     8101 tgacctctat gttcgaaatg attttgtaac tggggtggcc attgcttttc aagattacaa
     8161 ggaagtctat gtttctacta aagacaaacg tagtggagaa aaaatcaggg ctgaggattt
     8221 caaaccagca ggaaatagtt ttgccattcc ggtgtcagat ccagtgtcag atcaagattt
     8281 aggagtgatt tacatctcct tggatcctgc tgttttatac catgccattg ataatactag
     8341 aggtcatact ccgatggcag taacagtgac ctcacctttt gatacggaga tttttcatat
     8401 tggtgagaca gttgataggg agagtgaaaa ttggctagtt ggcttaactt ctcatggata
     8461 tcaggttcag gtggcagttc ctaaaaactt tgttttacaa ggaacagtga ctagctctgc
     8521 tttgattgtg ggtttgagcc ttctctttat tgtcattctt tatctgactt tgaggcagac
     8581 ttttgctaat taccaaaagc aggtagtgga tttagtagaa tccattcaag tcattgctca
     8641 aggcgaagag gggcgtcgga ttgacatttc cgagaaagat caggaattac tcctaatcgc
     8701 ggagacgacc aatgatatgt tggatcgatt ggaaaagaat atccatgata tttaccagtt
     8761 agagcttagt caaaaagatg ccaatatgcg agccttgcag gcgcaaatca atcctcattt
     8821 tatgtataat acgctggagt tcttgcgcat gtatgcagtt atgcagagtc aagatgagtt
     8881 ggcagatatc atttatgaat tcagtagtct cttgcgtaac aatatttccg acgaaagaga
     8941 gaccctcctc aaacaggaat tagaattttg ccgtaaatac agctatctct gcatggttcg
     9001 ctatcccaag tccattgcct atggtttcaa gatagatcca gagttagaga atatgaagat
     9061 tcccaagttt accttgcaac cgctggtaga aaactatttc gcgcatggtg ttgaccacag
     9121 gcggacagat aatgtgatta gcatcaaggc tcttaaacag gatggttttg tggaaatttt
     9181 ggtggtcgat aatggtagag gaatgtcggc tgaaaagttg gcaaatatcc gagaaaaatt
     9241 aagtcagaga tattttgaac accaagccag ctacagtgat caaaggcagt ctatcgggat
     9301 tgtcaatgta cacgagcgtt ttgtgctcta ttttggagac cgctatgcca ttactataga
     9361 gtctgcagag caagccggtg ttcagtatcg tattacaatt caagatgagt agaaagggag
     9421 aaaatgtata aagtattatt agtagatgat gagtacatgg tgacagaagg tctgaagcgt
     9481 ttgattccct ttgataagtg ggatatggag gttgtcgcaa cagccagtca tgccgatgaa
     9541 gctctagaat atgttcagga aaatcctgtc gatgtcatca tttccgatgt caatatgcca
     9601 gacaaaacag ggcttgatat gattcgggag atgaaagaga tcttaccaga tgctgcctat
     9661 atcctgctct caggttatca ggagtttgat tatgtaaaaa gagcaatgaa ccttagtgtg
     9721 gtggactatt tggtcaagcc tgttgataag gtagagctgg gaaatctgct ggagaagatt
     9781 gcaggtcagc tcggcgagag agggaagaaa agtcagactc ttagtcaaga attagacgag
     9841 gctggatttg ttagttattt aggggataag gagaattggt ggataggtct atccaaggaa
     9901 aaacaaggtt ccttcaccat tccctactat gtcttgggcc aagactggca gattttcatt
     9961 tctggccaac ctctagatgg tttagtcgtt acaccttttg aagctcctta tcaagaacac
    10021 tttgaacgct ggaagctgaa tgctgagaaa accctctttt acggttctgt aaatttgcag
    10081 cagtctgaga gtctctttgc ctattacgaa ccgatttata gggttatcat tcagggaaat
    10141 ctcaatcaaa tcgtagaaga gttaaatctc ttggagaagg tagttcttga aaatacgccg
    10201 cgtgttccga ttactaaaca gctttttatt cagtttgtca tggatgtttt ccatttattt
    10261 gaacatctca aagctgatga cctgacagac atcgtcaaaa ccattcatgc tattcaatcc
    10321 ttcgatgaat tggtttctta tatcaaggaa actctgatca gctttttcgg tcaataccgt
    10381 atgaatgaaa atgtggtcag tgtgctggaa gtcattggtc gtgattacca aaaagagctt
    10441 tccctcaagg atatcagtaa ggctctcttt atcaatcctg tctatctagg gcagttgatt
    10501 aagcgtgaaa ccgattcgac ctttgcagag ttactaaaca aacaacgtat taaggctgcc
    10561 caacaacttt tgctttcaac tagtgacagc atcgaagata tttgttatgc tgttggttac
    10621 agtaaccttg gatatttcta taaagttttc cgaaaattgt gcggaaaatc gccaaaagcc
    10681 taccgaaaac aggtagaaac tatactataa aatttgtatt cctttacaaa aggtgctata
    10741 atattatcag gaggttctat catgaaaaag aaaccaattt atctatgggt cttgctaatc
    10801 ttgtctgccc ttatttcagt tccgtctctg tttggaattg tgagtccctt gcctagtaag
    10861 gaagcccttc gtgctgctca gaaacaagtt gcaggggtca atgctcagca attagaagac
    10921 cagcttaatt atacctatag agtagcagaa gcatctcatt ctatttttaa tgttgctttg
    10981 attgtgctat ctactatttt agtggtcgta gcgattgtct tccttgttcg taaaaatttg
    11041 caatatgcaa actatactta cgttggctat gttttgttag ctattattgg ttcgatttat
    11101 ggctatgttg gtttacaaga cgctgtgcag ttggttcaag atgagagcat gcgtttgaca
    11161 gtgagtattg gttcaaaagc tgttagcatt ttctatatcg tcatcaatgt tctcttccta
    11221 gcccttgtct tctataagat gtggcgacaa cagaaagcct tggcagaaga agaggaaaca
    11281 gaagagcttg cctaagtatt gacaaaaaag aactatcaag atacaatctt ggtagttgtt
    11341 ttttgattat agataaaatc atggaggtac atatgaagac aatttcttta gtttatatta
    11401 gtttgagtgg caacactgaa agttttgtga cgcgcttgaa agactatctc ttgtcccagt
    11461 acaaagggat tgaggttcaa aagattcata tcaaggattt agtcaaggaa ggcaaaaatt
    11521 tctatgaaat ggaccatccc tatgtcgctt ttttgccgac ctatctcgaa ggtgggaatg
    11581 gcgtggataa cggagatgtt gagattttga cgacaccggt gggagatttt attgcctatg
    11641 gtaacaatgc tagtaagtgt tttggtgtgg ttgggtcagg aaatcgtaac tttaataacc
    11701 aatactgcct aacagccaag caatacagtc aacgctttgg ctttcctgta ttggctgact
    11761 ttgaaatgcg aggcatgctg gaagatatca aacatgtcgc agctattatc gcagatttgt
    11821 atgagttgga aaaggagaat taatactcaa tgaaaatcaa agagcaaact aggaagctag
    11881 tcgcaggttg ctcaaagcac agctttgagg ttgcagatag agctgacgtg gtttgaagag
    11941 attctcgaat agtataaagt tcttataaga ggtggttgaa gttgttcaac tgttttttga
    12001 gtataaacag tctttaagaa cgtaaaaaag cataagttca ggtcttcaaa accttgttct
    12061 tatgcttttt atcatagaaa tatttgctga attttctctt gaaatgaaac cttatcttca
    12121 tctcgcgctt gtcccttacc ctgcttttcc gattgcgagt gcgtagataa agaagatggc
    12181 gaagccaaag aggaaaatca atcccgcaat ggcaaggtgt ttgagccaag aaggaagatt
    12241 tttgttttca cgcgttacca aggcgtaatt caaaagagca aagaaaggtg ttgtcaggaa
    12301 agagccaatc atggcaaagc ggagcatggt tgaaacctga ccagcgaaga acttgataat
    12361 gacgataccg atgatagcag tgatggtcat ccagatgttc aaagatttac gattgtcctc
    12421 tttttgactg attagcagtc ggagagattc ctgattaacg cgagaatagc catcgataac
    12481 agttataact gttccaaaga tacagaggaa ggcaataaag gtaatcaagt aacgggacca
    12541 ttcgccaaga acagaggcat acatgcccac gaattgagag atgtatttgg ctgaagcagc
    12601 ttcaaccgcc tgccctgtag gatactgaat cagtgctccc agtgccacaa agaagacggc
    12661 taggatagct gttccaatat aaccagtgtt aaagtcaaac acagcgtctt ctgtgttaaa
    12721 gttgacggtc tttctctttt cagctgacca aagtgaattg atggctgaaa tttcaatagg
    12781 agccggcatc catcctagga gggagacgat gaagggcaga gctgccattt gccaaggtgt
    12841 cttctcgaca aaatcagaac tgtattctgg atgcttgacc gccgcaatga taactgcaag
    12901 aacagtcgca atggttaagg cagacataat ccatttgacc atgccgtcta aaagtttgta
    12961 gcctccaaag agtagcatag cccaaatgat tgcaacgaga atgagggacc actgagtaat
    13021 gctaagtcca atcattggga aggcactggc gatgatagct gagcacagaa tggcaacacc
    13081 agccgtgttg accatagccg aaaagacatt gaggataaag aaaatccaga gatagagttt
    13141 tcctttttcg gcataacctt caaccaaagt ctttccagta tcagctgtgt attcagcacc
    13201 aaaacggaaa aatggatatt taaagacatt ggctaagatg accaagagaa gtagagacca
    13261 accgtaagaa ccgccagctt gagttgagga tacaatgtgg gaacctccaa cagcggcagt
    13321 tgccattagg attccaggtc ccattgcttt tagcttgctt gcccaggttg attgatttaa
    13381 ggaaatagct tgcgacatga taaatactcc tttttgaatt ttcagaataa tctgtatatg
    13441 taatctatat tctacaaaaa attttggaaa aatgcaagag tattttgaaa aaagatagaa
    13501 aatttacaaa gaaaatctga aataactgtt atgatatagc agattgaaac tagaatagta
    13561 cacttttgct tctaaaacat tgttagaaat cgatttgact gtcctgattg atttaccctg
    13621 ttcttatttc atttcactat agtaaggtgc agcaggataa agcaaaaccg agaagtttct
    13681 ttctcggttt tgcttattat gaggttccta ttttctatgc tccaagagtt gattgatgtg
    13741 atctactttt ttagcctctc ttttatagag aatagcccag atgatgagat agacaatcgc
    13801 aaactcgata atgagctgga ggtagaaaat ccagtggaag gggaaccaac cagcaagagt
    13861 tgctagtggg acaaagccag ccagcataag gaagaaatga gtcagagtgg cacgaagcat
    13921 gctccagtca cggctgaata agcggttgcc aaagttgaag agcataccga tggctgccca
    13981 gataagtgtg cagtagagca agaccagggc accgtgaacc tgatgctgat ccatcacttg
    14041 gccgatgaga gagtagggat ttagtggtgc gtaggtattt ggtgcataaa tgagtgaaaa
    14101 gaggatggag aggatgaggc cgataagaac accggcagct gcatcgtgaa atacttgttt
    14161 tttcatagtt ctaatttctc cttgatggtt tttagataac ggcgtgaaga gtaggtgaag
    14221 ctttcgtttt tcaagaaaat ttctaccaga ccgtttggcg tgagcttgag gtgagagatg
    14281 gaatcgatat tgatgatttc tgattgggaa atttggataa aattggttgg caagagttta
    14341 agaacctgat agagtcgcaa atcaatgctg taggtctgac tcgcggtttc tgctagaacc
    14401 ttccgattct cgatatagaa gcgctgaatc ttgccaatct caactagata gacctgatca
    14461 tcgatttttc ctttgatttt ttctctttgg tccagatttt ctgcgaactc gatgactttc
    14521 tggacttttt cggtttcttg aggtgcttgg acaatcagct tttcctcctc gtaagtctca
    14581 ctaatctgta gttctacttt cataaatttc tctccttgtt tttcatacaa aattaatctc
    14641 acttcttgta cacctttatt aatactcttc aaaaatctct tcaaaccacg tcagcgtcgt
    14701 cttgccgtag gatagttgtt gactttgtca gttttatcta caacctcaaa acagtgtttt
    14761 gagctgtctg cgtctagctt tctagtttgc tctttgattt ttattgagta taccactatt
    14821 ttactccctc tggcaaggga ctttgtctat gtggagggat tgggctccta tgtggtggag
    14881 tttttctgtt ctttctgaaa tatggtataa tagcactaat taatttctag gaaaatagat
    14941 acagaaaggg gctgaaagat gtctcatatt attgaattgc cagagatgct ggcaaaccaa
    15001 atcgcagctg gggaggtcat tgaacgtcct gccagtgtgg ttaaagagtt ggtagaaaat
    15061 gccattgacg cgggctctag tcagattatc attgagattg aggaagctgg tctcaagaag
    15121 gttcaaatca cggataacgg tcatggaatt gcccacgatg aggtggagtt ggccctgcgt
    15181 cgccatgcga ccagtaagat aaaaaatcaa gcagatctct ttcggattcg gacgcttggt
    15241 tttcgtggtg aagccttgcc ttctattgcg tctgttagtg tcttgactct gttaacggcg
    15301 gtggatggtg ctagtcatgg aaccaagtta gtcgcgcgtg ggggtgaagt tgaggaagtc
    15361 atcccagcga ctagtcctgt gggaaccaag gtttgtgtgg aggatctctt tttcaacacg
    15421 cctgcccgtc tcaagtatat gaagagccag caagcggagt tgtctcatat cattgatatt
    15481 gtcaaccgtc tgggcttggc ccatcctgag atttctttta gcttgattag tgatggcaag
    15541 gaaatgacgc ggacagcagg gactggtcaa ttgcgccaag caatcgcagg gatttacggt
    15601 ttggtcagtg ccaagaagat gattgaaatt gagaactctg acctagattt cgaaatttca
    15661 ggttttgtgt ctttacctga gttgactcgg gctaaccgca attatatcag cctcttcatc
    15721 aatggccgtt atatcaagaa cttcctgctc aatcgtgcta ttttggatgg tttcggaagc
    15781 aagcttatgg ttggacgttt tccactggct gtcattcaca tccatatcga cccttatcta
    15841 gcggatgtca atgtgcatcc aactaagcaa gaggtgcgga tttccaagga aaaagaactg
    15901 atgaccctgg tttcagaagc tattgcaaat agtctcaagg aacaaacctt gattccagat
    15961 gccttggaaa atcttgccaa atcgaccgtg cgcaatcgtg agaaggtgga gcaaactatt
    16021 ctcccactca aagaaaatac gctctactat gagaaaactg agccgtcaag acctagtcaa
    16081 actgaagtag ctgattatca ggtagaattg actgatgaag ggcaggattt gaccctgttt
    16141 gccaaggaaa ccttggaccg attgaccaag ccagcaaaac tgcattttgc agagagaaag
    16201 cctgctaact acgaccagct agaccatcca gagttagatc ttgctagcat cgataaggct
    16261 tatgacaaac tggagcgaga agaagcatcc agcttcccag agttggagtt tttcggacaa
    16321 atgcacggga cttatctctt tgcccaaggg cgagatggac tttacatcat agatcagcac
    16381 gctgctcagg aacgggtcaa gtacgaggag taccgtgaaa gcattggcaa tgttgaccag
    16441 agccagcagc aactcctagt gccctatatc tttgaatttc ctgcggatga tgccctgcgt
    16501 ctcaaggaaa gaatgcctct cttagaggaa gtgggcgtct ttctagcaga gtacggagaa
    16561 aatcaattta ttctacgtga acatcctatt tggatggcag aagaagagat tgaatcaggc
    16621 atctatgaga tgtgcgacat gctccttttg accaaggaag tttctatcaa gaaataccga
    16681 gcagagctgg ctatcatgat gtcttgcaag cgatctatca aggccaatca tcgtattgat
    16741 gatcattcag ctagacaact cctctatcag ctttctcaat gtgacaatcc ctataactgt
    16801 cctcacggac gtcctgtttt ggtgcatttt accaagtcgg atatggaaaa gatgttccga
    16861 cgtattcagg aaaatcacac cagtctccgt gagttgggga aatattaaaa gtataaaaaa
    16921 gtctgggaaa aattttcaaa atcagaaaaa cgcataaaat caggtgttca aaaaccttga
    16981 ttttatgcgt tttatcatag aaatatttac ttcatgtttt cctaattctt ttcgaaactc
    17041 tttttaaacc acgtcagttt tatcagtaat ctcaaaacag tgttttgagc taattttgcc
    17101 agttttgtct gtaacatcga agttgtgttt gaccactctg cgactggttt cctagtttgc
    17161 tctatgattt tcacagagta ttaaattgcg attttgccaa gtttctttat tcgtctaaaa
    17221 gtagagtctg ttctatgcgt ctaatgtacg aattaggttg accatttcaa tagctccttg
    17281 tgcacactca gaacccttat ttcctgcttt agtgccagct cgttctatgg cttgttcaat
    17341 tgtatctgtc gttagcacac caaacataac aggaatttcg ctatttaaac tgatttgggc
    17401 gattccctta gatacctcgc tacatacata atcataatga cttgtattcc ctctaatgac
    17461 agctcccaag cagataattg catcatattt tttacttttt gccatttttg atgcaatcag
    17521 tggtatttca aaagctcctg gaacccaggc tacctctata tctttctcgt ttacattctc
    17581 tcttttgaga ttatctagtg ctccagataa taattttgaa gttataaatt cattaaatct
    17641 cgctacaaca atacctattt taatattgtt tgctactaaa ttaccttcat aagtgttcat
    17701 ttatttttcc tccatattta aaatgtgacc cattcgattt ttctttgttt ctaaataaaa
    17761 actatcgtaa ggattggctt ctatttcgat tgatattcta ctggaaatgg taattccata
    17821 tttttctaac tgttcaacct tgtcaggatt atttgtcagt aaatgaagtg actgaagtcc
    17881 cagatcttta agcatttgtg ctccaatatg atattctctt aaatcacctt caaagcctaa
    17941 tgcaagattg gcatcaagcg tatccatgcc ttgatcttgt aaatgatagg cttttaattt
    18001 attgataagt ccaattcctc gtccctcctg tcgcaagtaa agtaagacac ccgaaccatt
    18061 ctcaacaatc attttcatag ctttatcgaa ttgctgtcca caatcgcaac gtaaggagcc
    18121 taaaacatcc cctgttaaac attcggagtg gacccgacat aatacattgg cttcatcctc
    18181 tatgtttccc ataataagag caagatgatg ttccccattt agtttatcta tatagctaat
    18241 tgctttgaaa ttaccgtatc tagtaggcat attgacagtt gaaactcgtt ctaccagctg
    18301 atcatatact tttctatatt cttgtaattc tttgatggta attagtggaa tgttgtgttt
    18361 cttcgagaac tgaattaaat catctgttct catcattttg ccatcatgat tcattatttc
    18421 acaacatagg ccacactctt ttagtccagc taattttaat aaatcaacag ttgcttctgt
    18481 gtgtccactt ctttctagaa caccaccttt ttttgcaatt aaaggaaaca tgtgtcctgg
    18541 cctgcgaaaa tcagagggtg ttatatcttc agctacacac atacgtgcgg tcagtcctct
    18601 ttcctcggca gaaatacctg tggtcgtttc tttataatca attgaaactg taaaagcagt
    18661 cttatgatta tctgtattgt tttcaaccat aggtgaaagc attaattgat tagctaaact
    18721 ttcgctcata ggcatacaaa ttaatccttt ggcataagta gccataaaat taacattttc
    18781 tgttgtagct gcttgtgcag aacaaattaa gtctccttca ttttctctat ccttgtcgtc
    18841 tataacaaga acaagtcgtc ccttctgcaa tgcttctaat gcttcttgta tttttcgata
    18901 ttccattgac tgattatcct ttctgctaaa atccattttg atataatagt tccttagata
    18961 tttctgattt tggagagtta tccatcagtt tttgcacata tttacctaag atatcatttt
    19021 caagatttac tgtactcccg acttgtttac tcttaagaat ggtttgttcc aaggtatgag
    19081 ggataacaga tactgaaaag tttactttgg agactttagc gacagtcaga ctaatgccgt
    19141 caattgtaat agatcctttt tcaactatta aatctaaaat ttctttttgt gtgttgattt
    19201 gataccatac agcattatca tcttttttta ttgacgagat ttttcctgta ccatcaatgt
    19261 gtcctgtaac gacgtgaccc ccaagtcgac cgttgacaga taaggctctt tctagattca
    19321 cctcacttcc atgttttaat agagtaagag ctgttcgact ccatgtttca ttcattacat
    19381 caactgtaaa ggattgatga ttgaaatgag taactgtaag acagatacca tttactgcta
    19441 tactatcgcc taaatggata tccgttaata tttttgaggc tttaattgat agtttacaat
    19501 tacgagagtc tttctgtatt ctttcaactt ttccgatttc ttcaattatt cctgtgaaca
    19561 tggataaatc acttcacttt ctatgagata gtcatttcct atttgagaaa atgcataagg
    19621 tttcaatcta atagcgtcat ttggcaaaga aataccttca cctccgacag gaaacttggc
    19681 actacctcca aaaatttttg gtgcaatata tattttcagc tcatcaacaa tttgttgttc
    19741 caaagcactc caattcatta gactgccccc ttctagaact aggctatcaa tctgcatgtt
    19801 tcctagatgt tgcattaaac tcgataagtc tatatgattg ccttttttct ttatggaaag
    19861 tatttcacag ccatgatttt gatatagctt cattttattt ttgtcttcag aggaagtggc
    19921 aatgtaagtt ttaatatcat ttgctgtttt tacgatttta gaggtaagag gagttcgtaa
    19981 atgtgtatcg catatgatac ggataggatt tttcccttcc tccaatctac atgtcagcaa
    20041 aggatcgtct tgaataacag tattgactcc caccataatt gcactaacat ggtgtcgtaa
    20101 ctgctgcaca tgctttcttg cttcttcttc agtaatccat ttggattgat ttgttttagt
    20161 ggctattttt ccatccattg acattgcata tttcataaaa acatagggta catgctgggt
    20221 aatatacttt ctaaaacttt ttattaagtt aagacactca ttttctaaaa ttccaacagt
    20281 aacttgaaga ttattttcct caagtatctt tactcctttt ccagatacaa taggattaca
    20341 gtctaggctt ccaatgacta ctcttgtaat accactatcg attatagcat ctatacaggg
    20401 aggtgttttc ccgaagtgac aacagggttc aagtgttaca taaagcgtcg ctccgacagg
    20461 ggattctcta cagtttttaa gagcatttct ctcagcatgt gggccaccaa aaaactcatg
    20521 ataaccttgt ccgataatgt gattatcttt tacaataact gcgccgacca taggattggg
    20581 attgacgtaa ccagcccctt tttgtgccag ttttattgct aatttcatat attttgaatc
    20641 gctcatctca ctacctccaa aaaaatatgc cttgaatagg ggactactca aggcatacaa
    20701 aagaaaactt atgcgattaa caaaaatgct ctgaaatgac aagtaatcat ttcagagcac
    20761 gcaaaaagca caaatatact tttatcttct ttcatccaga ctatactgtc ggctttggaa
    20821 tttcaccaaa tcatgccttt cggctcgtgg gctataccac cggtagggaa ttgcaccctg
    20881 ccctgaagat agttattcaa ttacagatga ttatagtact taattttgaa tatgtcaaca
    20941 gataaatacc gattgttttt gatatactgt atttgtgata atcgattctc gctcctcgga
    21001 taaagaaaat atgatatact agataaacga aataagagag aaggaatact atgtacgcat
    21061 atttaaaagg aatcattacc aaaattactg ccaaatacat tgttcttgaa accaatggta
    21121 ttggttatat cctgcatgtg gccaatcctt atgcctattc aggtcaggtt aatcaggagg
    21181 ctcagattta tgtgcatcag gttgtgcgtg aggacgccca tttgctttat ggatttcgct
    21241 cagaggatga gaaaaagctc tttcttagtc tgatttcggt ctctgggatt ggtcctgtat
    21301 cagctcttgc tattatcgct gctgatgaca atgctggctt ggttcaagcc attgaaacca
    21361 agaacatcac ctacttgacc aagttcccta aaattggcaa gaaaacagcc cagcagatgg
    21421 tgctggactt ggaaggcaag gtagtagttg caggagatga ccttcctgcc aaggtcgcag
    21481 tgcaagcaag tgctgaaaac caagaattgg aagaagctat ggaagccatg ttggctctgg
    21541 gctacaaggc aacagagctc aagaaaatca agaaattctt tgaaggaacg acagatacag
    21601 ctgagaacta tatcaagtcg gcccttaaaa tgttggtcaa ataggagcag agaatgacaa
    21661 aacgttgttc gtgggtcaag atgaccaacc cgctctacat cgcctatcat gatgaggagt
    21721 ggggccagcc cctccatgat gaccaagtat tgtttgagtt gttgtgtatg gaaacctatc
    21781 aggcaggcct gtcttgggaa acggtactca acaaacgcca agctttccga gaagtctttc
    21841 atagctatca aattcactca gtcgcagaga tgactgacac tgaattggaa gccatgctgg
    21901 agaatccagc tatcattcga aatagagcca agctttttgc tacacgcgct aacgcccaag
    21961 tctttctaca gttacaggca gagtacggct cttttgatgc ctatctttgg tcttttgttg
    22021 aggggaaaac tgtcgttaac gatgttcctg attatcgcca agcgccagct aaaacaccct
    22081 tatctgagaa attagccaaa gatctcaaaa aacgaggctt caagttcaca ggcccagtcg
    22141 ccgtattgtc ttttctacag gctgcagggc tagttgatga ccacgagaat gattgtgagt
    22201 ggaaaggtct taaatgatgt ctaacaaaaa taagggaatt ctgatttttg cgattctcta
    22261 tacagtcctc tttgtgtttg atggcgttaa attgctggct tctttaatgc catctgtcat
    22321 tgcaaattat cttgtttatg tagttttagc tttatatggc tccttcttgt tcaaggatag
    22381 attgatccaa caatggaagg agattagaaa gactaaaaga aaattcttct ttggcgtctt
    22441 aacaggatgg ctctttctca ttctgatgac tgttgtcttt gaatttgtat cagagatgtt
    22501 gaagcagttt gtgagactag atggacaagg tctaaatcag tctaatattc aaagtacctt
    22561 tcaagaacaa ccactactga tagctgtttt tgcttgtgtc attggacctc tggtagaaga
    22621 attatttttc cgtcaggtct tattgcatta cttgcaggaa cggttgccag gtttactaag
    22681 cattattctg gtaggacttg tttttgcttt gactcatatg cacagtttgg ctctatcaga
    22741 gtggattggt gcagttggtt acttaggtgg aggccttgcc ttttctatta tttatgtgaa
    22801 agaaaaagag aatatctact atcccctact tgttcacatg ttaagcaaca gcctctcctt
    22861 aatcatttta gctatcagta tagtaaaatg aaataagaac aggacaaatc aatcaggaca
    22921 gtcaaatcga tttctaacaa tgttttagaa gtagaggtgt actattctag tttcaataca
    22981 ctgtaacatg tgatgaaaat gccagtaatg ataccgagaa aaaagctgag aaacttttcc
    23041 cagctttatt tgttatagtc aaagcgaatg acttgttcct gtgcatctac atgagcatgg
    23101 accccaaagg gtacaattgc tcttggagtt gcgtggccga cattcagatt atagacaatc
    23161 gggatattgc tgtcaatgat atctaatagt gcctctttat agtcgtcatg gaaagtttca
    23221 tccataggtt ttccgaccaa gagtccattg atgaccgcga atatgccagt gtcctttaaa
    23281 gttagcaaca tctttttgaa gtcttctggc ttaggctttt cttcgcttgt ttcgagcaag
    23341 aggattttcc cttcccagtc tgacaagtca gggaaaagtt tgtattttgg gcagagttcc
    23401 gtgctatctg cgtatcgaga gttgtcaaag atatcgtaga gggattcgag gcaaccaccg
    23461 aggattttcc cctcgaactg agcacttcct tgcaacaagt caaaacctgt atttgtatga
    23521 ctgacacgag gtgttcccag ggccgtggga ctaaaatcag ttcgttcctc ataccaaacg
    23581 tcactagggc ggatttctga aattcttccc gtctcaatca attctttaaa gtagtgaagg
    23641 ctataggcta gcatttcttt gtctaattca caaatgtctg ctaaaaagga ttgaccataa
    23701 aaagtcttga ttcctaattt atgcaacatg aggtggttca tggttgtatc cgagaagcca
    23761 agaaaaattt tttgcttgat aaccttttgg agttggtcat tttcaaaaag ataaggtagc
    23821 aagcgatagg tatcgtctcc accgatggca cataggatca tgtcgatgct atcatcagaa
    23881 aaggcatgaa tcaaatcctc tgcacgagct tcaggatggt ccttgataaa gtctaatcct
    23941 tttaacgaat ggggcaaaaa gatgggattg agtcccagat ccttgagacg ttggataccc
    24001 aagtccactt cgtgtttgac aaaatcctct ccgataatgc cactagataa actaacaata
    24061 ccaatagtag aaaccatatc tcatcctcct agaaatagat tgagcctatt ttatcacaaa
    24121 atataagaga aagctatgtg ggatttcaga agaatacttt taatactctt cgaaaatctc
    24181 ttcaaaccac gtcagtttta tctgcaacct caaagctgtg ctttgagcaa cctgcagcta
    24241 gcttcctagt ttgctctttg attttcattg aatataactc aaaaaagtaa gtgaaaaagc
    24301 aaccgcacct gcagttgctt ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac
    24361 ttgcactacg gatattcaag cgtttcttga gatagaagcg aagggctagg agggctgctc
    24421 caataatagc tagtggcaat ggatccagta ctgggttaag gctagctggt aggaagcttg
    24481 ttgcaaagaa gacaagcaac caaaggaaca tagaagctag gataactagt acagatttcc
    24541 agaaaggtgg acgttgactg cgatccatat ctggtccata gtattggtaa acaaagtagt
    24601 acatcaagta gaaggcaaat ccaccaacca gtccaactaa tagaagagta atcaatccat
    24661 agccgaaagc ttgatctgcc gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc
    24721 tagtgatgaa aagagctgag tccataatca tcagttttgg atcatcattt tcttttggat
    24781 gctctttttc gtactgctct ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt
    24841 atagggaacg ggcagttgta cctttagatt gctcctcaag gatttgggga atgacttcct
    24901 caaaaatagt ctggatttca gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa
    24961 tgtggacaaa ctcttggttt ttcttagtta atttttgtag atcaatttga gacataggaa
    25021 ctcctcttag aaccattttt tatggatgag atagagagtg agcgagacac tcatagcaaa
    25081 ggcgataaag acgattaacc agaaggcatt tggctctccg tttaggggga tttcattatc
    25141 cttaaagttc atcccgtagg cagaaaagac catggttggg atggacatga cgatggtcac
    25201 aagggccaag gttttcatga tgttgttctg gttgttagaa atgatagagg caaaggtctc
    25261 tgtcatagaa tgcaagacgt ttccataaat atctgccatc tcgatggcct gttgggtttc
    25321 aatcagggtg tcttcaagca ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga
    25381 actggtcaat ttcttaatca cgcgctcatt tgttttgagg gaggccttga aatagacgat
    25441 agttttttcc aattccatga gctcaatcaa ttcttcatta cgagttgatt gatgcagttg
    25501 actttcgatt tgttcactct tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc
    25561 attgcgataa agaatttgaa agataaaacg tgaacgcatg aaggtataga aattacgcaa
    25621 tcgacggttg ataaagacat caaggacagg tagtggttcc aaacacgtag tgataatggt
    25681 ttcctcagtg atgataatac caagcgggat ggttacgtag taggtgcggt tatttctttc
    25741 ctccgtgacc ggcacgtcta cgataatcag ggtatactcg tcttcaatgg taatacgaga
    25801 catttcttcc gcatcgagcg gtgctcgaag gtcggcaata tcaatatcga aggtgttggc
    25861 gatttcgagt gattcatttt gagtcggatt gacgagattg atccaagtac ccggttcaag
    25921 cgtatcgatc tctttaaatt cagttgttgt agagagaaaa acttgtttca tatcccttat
    25981 cctttctcat ttttcagatt ttttcacact gtactattat actacaaaat cggccttttg
    26041 ggtaatagaa tctcactttt tttggtataa tggtaagcaa taatggacta gaaagaacaa
    26101 agatgcaaga taaaattgtc attcatgggg cgcgtgccca taatttaaaa aatattgatg
    26161 tggagattcc gcgagacaag ctggttgtcg tgacaggttt gtcaggttca gggaaatcca
    26221 gtctggcctt tgataccctc tatgcggagg gacaacgtcg ctatgtagag agtttgtcag
    26281 cctatgctcg tcaattctta ggaaatatgg agaaggcgga tgtggatgcc attgatggtc
    26341 tcagtccagc tatttctatc gaccagaaaa cgactagcaa aaaccctcgc tcgacggtgg
    26401 gaacaacgac tgaaatcaat gactatctgc gtctcctcta cgcacgtgtg gggacgcctt
    26461 actgtatcaa cggacatgga gctatcaatg cttcttctgt ggagcaaatc gttgataagg
    26521 ttttggagtt acctgaacgc cagcgcttgc agatcttggc ccctgtcatc cgcaagaaaa
    26581 aaggccaaca taagagtgtt atcgagaagg ttcagaaaga cgggtatgtt cgtgtccgtg
    26641 tggatgggga agtctatgat gtgaccgaag tgccagagtt gtctaagagc aagcaacaca
    26701 atatcgatgt cgtggttgac cgtattgtta tcaaggaggg cattcgtagt cgtctctttg
    26761 attccattga ggctgccctt cgtattgcag aaggttatgt cattatcgat accatggacg
    26821 actctgagtt gttgttctct gagcattatg cctgtccagt ttgtggtttt actgtcccag
    26881 agttagagcc tcgtctcttc tctttcaatg ctccttttgg ctcttgtagt gagtgtgacg
    26941 gcttgggcat caagctggag gtggatactg atttggtagt gccagatgcc agcaaaacct
    27001 tacgtgaggg agcgttagca ccttggaatc ctatctcatc caactactat ccaaacatgt
    27061 tagagcaggc catgaaagtc tttggagtgg ctatggataa gccttttgag gacctgtcag
    27121 aagaagataa gaacttgatt ctctatgggt cagatggtaa ggaattccat ttccactatg
    27181 agaatgaatt tggtggcgtg cgcgatatcg acattccgtt tgagggagtt atcaataata
    27241 tcaaacgtcg ttaccatgaa accaatagcg attacactcg cactcagatg cgtctctaca
    27301 tgaatgagct gacctgcgga acctgtcaag gctatcgtct caatgaccag gccttgtctg
    27361 tccgtgtggg cggccagcaa gggccacata ttggagaaat ctcagacctg tctatcgctg
    27421 accacttgga cttggtgagc cagttaactt tgtctgaaaa tgaagccatc attgctcgtc
    27481 ccattctcaa ggaaatcaag gatcgtttga ccttccttaa taatgtgggt cttaactatc
    27541 taaccctgtc acgttcagca ggaacccttt caggtgggga aagtcagcgc attcgtttgg
    27601 caacccagat tggttccaac ctatcaggcg tcctctatat cctagacgag ccgtcaatcg
    27661 gtcttcacca gagagacaat gaccgcctga ttgccagtct gaaaaagatg cgtgacttgg
    27721 gcaatactct tatcgtggtg gaacacgacg aagataccat gcgtgaagct gattatctga
    27781 ttgacgttgg tcctggtgcc ggtgtttttg gtggtgagat tgttgcagca ggtacaccta
    27841 agcaagtggc tcgcaacagc aagtctatca caggtcagta cttgtcaggc aaacgtgtca
    27901 tcccagtacc agaagagcgc cgtgtcggaa atggtcgttt tattgaagtg ataggagcgc
    27961 gtgagaacaa cttgcaaaat gtcacggctc gctttccact aggaaaattt atcgcagtga
    28021 caggtgtgtc gggttcaggg aaatcgactc taatcaacag cattcttaaa aaagccattg
    28081 cccagaagct caaccgtaat tcagacaaac ctggtaagtt taagactatt acagggattg
    28141 agcatgtcga ccgtttgatt gatattgacc agagccctat cggacggacg ccgaggtcta
    28201 acccagctac ctatacagga gtttttgacg atatacgtga cctttttgct cagacaaatg
    28261 aagccaagat tcgaggctac aagaaggggc gcttcagttt caacgtcaag ggaggtcgct
    28321 gtgaagcttg ctcaggtgac gggattatca agattgagat gcacttcttg ccagatgttt
    28381 atgtggcttg tgaagtttgc cacggaaccc gctataacag tgaaacccta gaagttcact
    28441 acaaggaaaa gaatatttcg caggtcttgg atatgacggt caacgatgcg gtagaatttt
    28501 tccaacacat tccgaaaatt caacgcaaac tccagaccat caaggatgta gggctaggct
    28561 atgtgacctt gggacagcca gctaccaccc tttctggggg agaagcccaa cgtatgaagt
    28621 tagctagcga actccacaaa cgctcgacag gaaaatcttt ctacattctg gatgagccga
    28681 cgacagggct tcatacagag gacattgctc gcctgctcaa ggttttagct cgctttgtag
    28741 acgatggcaa tacagtcctc gtcatcgagc acaatctgga tgttatcaag acggcagacc
    28801 atatcattga cttgggacct gagggcggtg tcggtggtgg aaccatcatc gtaacaggaa
    28861 ctccagaaga agtagctgcc aatgaagcca gctatacagg acactatttg aaaggaaagt
    28921 tacatcatga ataaacgcgt acaagcattt ctagctaaaa tgcaagaaaa agaactagat
    28981 ggtatcatca tcaacaatct taaaaacgtc tattatttga ctggtttttg gggctcaaac
    29041 ggaacagtct ttatcagccg tgaccgtcag gtcttagtga cagactctcg ctatatcatc
    29101 gcagctaagc aagaaaccag tggttttgag attgtggctg atcgtgatga attggctgtc
    29161 attgcaggaa ttgttaagga catgggcttg actcgtatcg gttttgaaga tgagatttca
    29221 gtatcttatt accaccgtat gcaggcagct tttgcaggtt tggacttgct tccacaaact
    29281 caatttgtgg aaggtcttcg tatgattaag gatgaggcag agattgcagc gattcgcaag
    29341 gcttgttcta tctcagacca agctttccgc gatgcgcttg actttatcaa accaggaaaa
    29401 actgaaattg aaattgccaa cttccttgat ttccgcatgc gtgagttggg agcatctagc
    29461 ttatcttttg atacgatcct agctagcggt atcaattctt ctaaacccca tgcccatcca
    29521 atgcacaaac cagtggagtt gggagaagcc attaccatgg acttcggctg tctctatgac
    29581 cactatgtca gtgatatgac ccggactatc tatctagggc atgttagcga tgagcaggca
    29641 gagatttaca atacggttct aaaagctaac caagccttga ttgaccaagc taaggcaggc
    29701 ttaggtttcc gtgactttga caaaatccct cgtgatatta tcattgaggc aggttatggc
    29761 gactacttta ctcacggcat tggccacggt attggtctgg atatccatga ggaaccctac
    29821 tttagccaga cttctacaga aactattaag acaggtatgg ccttgaccga tgaaccaggt
    29881 atctatatcg aaggcaaata tggcgttcgt atcgaggatg atatcctgat tacagagaca
    29941 ggttgtgaat tattgaccct agctccaaaa gagttgatag tcatttagtt gtttgtcaag
    30001 aaaaaagtac gaaaatgacg aaaaaagtca aaaaaaatta aaaataggtc gcaagtcgga
    30061 tgttttttat ggtataatag actaaactga tagtaacatg tagcgaaagg ggtaggtaca
    30121 tgattaaaat ttatacagtc tcaagttgta ctagctgtaa aaaagcaaaa acctggctca
    30181 atgcccacca gttaagttat aaagaacaaa accttggtaa agaaggaatt acgagagaag
    30241 aattactgga tattctaacc aaaacagata acggaatagc cagcatcgtt tcgtctaaaa
    30301 atcgctatgc caaagccctt ggagtggata ttgaagattt gagtgtcaat gaagttctca
    30361 atctgattat ggaaacaccg agaattttaa agagcccaat ccttgtagat gaaaaacgcc
    30421 tgcaagttgg ctacaaggaa gacgatattc gtgccttcct accacgctct gtccgtaatg
    30481 tagaaaatgc agaagcacgt ttacgtgcag ctctataaac atcaaggctg ggagcacttc
    30541 ccagtcttat tctattttaa tttcaaaaag aaagaagaga gaaatgaaaa aaatagttct
    30601 tgttagtcta gctttccttt ttgtcctggt tggttgcgga cagaaaaaag aaactggaac
    30661 agctacaaaa acagaaaagg atacgcttca gtcggcattg ccagttattg aaaatgccga
    30721 gaagaataca gttgtaacca agactttggt cttgcccaag tcagatgatg gtagccagca
    30781 aactcaaacc attacataca aggacaagac ttttttgagc ttaacgattc aacaaaaacg
    30841 tccagtttct gatgagttga agatttatat cgaccaacat ggagtggaag aaactcaaaa
    30901 agctcttctt gaagcggagg agaaggataa gtctatcatt gaagctcgta aattggcagg
    30961 tttcaaactt gaaacaaaac tattgagcgc aacggaactt caaacaacga ctagttttga
    31021 ttttcaagtt ctggatgtca agaaggcttc ccagttggaa catctgaaga atattggttt
    31081 ggaaaatctt ttgaaaaatg aaccaagcaa atatatttca gatagattgg caaatggcgc
    31141 gacagaacaa tagaaaatcc aaataaatag actgcctgaa ttgtagaagg taaggaatta
    31201 tgctataatg gtactattct aaggaaagag ggtgttagaa tgggatttac tgaagaaaca
    31261 gtacgtttta aattggacga ttccaataaa aaagaaatta gcgaaacttt gacagatgtt
    31321 tatgcttcgt tgaacgataa gggttacaac ccaattaacc aaatcgtagg ttacgtattg
    31381 agtggagacc ctgcctacgt tcctcgttat aataatgcac gaaatcaaat ccgtaagtat
    31441 gagcgtgatg aaatcgttga ggaattggtt cgctactacc taaaaggaca aggagtcgat
    31501 ctataaccta tgagaattat gggattggac gtcggttcaa aaacggtagg ggtggcgatt
    31561 agcgatccgc ttggttttac agctcaaggg cttgaaatca tccagataaa tgaagaacaa
    31621 ggccaatttg gttttgaccg cgttaaggaa ttggttgata cttacaaggt ggaacgattt
    31681 gtagtgggct tgcctaaaaa catgaacaat acaagtggac cgcgcgtaga agctagtcaa
    31741 gcctacggag caaagctaga agagtttttt ggtttaccag tagactatca ggatgaacgc
    31801 ttgacaacag tggctgctga gcgcatgttg attgaacaag cagatatcag tcgcaataag
    31861 cgcaagaaag tcattgataa gttagcagct cagctgattt tacaaaatta tttagataga
    31921 aaattttaat ataaaggaga ggctatgtca cacgatcata accacgacca cgaagaacgt
    31981 gaactaatca cactagtaga tgagcaagga aatgaaacct tgtttgaaat ccttttgaca
    32041 attgatggaa aagaagaatt tggtaaaaac tatgttcttc tagtaccagt taacgcagaa
    32101 gaagacgaag acggacaagt tgaaatccaa gcttactcat tcattgaaaa tgaagatgga
    32161 acagaaggcg aattgcaacc aatcccagaa gactcagaag acgaatggaa catgattgaa
    32221 gaagtcttca acagctttat ggaggagtaa aaacgtccgg gggacgtttt tagccctgcg
    32281 ctagaaatta gaaacgcagg tagtctggga gactgtatca gcccaactcc agaaatgggg
    32341 aagagttgga acgtccagtg aacattttta ccccatattc acattcatag tagctagtta
    32401 ttagctaacc aggtaagagg tcgggacgaa aatcctggcc ttgtttttat tagtaaccat
    32461 actttaatgc gataattgat tggacttttg tcaaggagat gtgtatgttt gaagtagaag
    32521 aatggctcca tagtcggatt ggtttgaatt ttcgatcagg tttgggccga atgcagcaag
    32581 cggtggattt gttaggaaat cctgagcagt cttaccctat tatccacgta acagggacta
    32641 atgggaaagg atctaccatt gcttttatga gggaattatt tatggggcat ggcaaaaaag
    32701 ttgcgacctt tacctcccct catatcgtct ctatcaatga ccgaatctgc attaatgggc
    32761 aacctatagc agacgcagac tttatccgtt tgactgatca ggtcaaggag atggagaaaa
    32821 cgcttctgca aactcctgcc cagttgtcct tttttgaatt gctgacctta gttgcttttc
    32881 tttattttag ggagcaagag gtggatttgg ttttattaga agtgggaatt ggtggcttac
    32941 ttgacacgac caatgtggta actggagagc ttgctgtcat cacctccatt gggcttgacc
    33001 atcaagaaac cttgggtgat agtctagaag caattgcaga gcagaaagct ggtattttca
    33061 aggctggtaa gaaggcagtg attgcgaaat tgcctccaga agctaggctt gtctgtcaga
    33121 aaaaagccga atctttagct gttaaccttt atcaggcagg tcaagatttt ttaatgctga
    33181 atggtgattt ttcaagctct ttactaaatc tttcacagct gaacataggc ttagaaggag
    33241 tctatcagca ggagaatgca gccttggcgt tgcaaacttt tcttcttttt atgggagaag
    33301 gaaaggaagc tgttgatgaa caggctgtaa gaaaggcctt ggaacagacc cattgggctg
    33361 gtcgcttgga gcgtattcgc ccacagattt acttggatgg tgctcataac ctccctgcct
    33421 tgactcgctt ggttgagttt atcaaagaaa aagagcagga aggctatcga cctcaaatcc
    33481 tttttggagc cttgaaacgg aaggattatc aagggatgtt aggttacctg actgaaaaat
    33541 tgcctcaggt ggaactcaag gtgaccggct ttgactatca gggggctttg gacgaaaggg
    33601 atgtgacagg ttacgatata gtttcttctt accgagaatt tatcagcgat tttgaagaaa
    33661 gagcagatgc tcaggatttg ctgttcgtta cagggtctct ctattttatc tcagaagtac
    33721 ggggctacct gctggaccgt gagcagataa attgaccccc ttgtttgaac ttgttatact
    33781 aggggaattg ctggttagag aaaatttctc taaattggta gcagaaagga aattcatcat
    33841 gaaattaaaa agattcacac tttctcttgc ttctctagca agttttagtc tcttagtagc
    33901 ttgttcacaa agagctcaac aggttcaaca gcctgttgct cagcagcagg tccaacagcc
    33961 tgctcaacag aataccaata ctgcaaatgc aggaggtaac caaaatcaag cggctccagt
    34021 acaaaaccaa cctgttgctc aaccgaccga tattgatggg acttatactg gtcaggatga
    34081 cggagaccgt atcactttag tggtaactgg aacgactggt acatggactg agctcgaatc
    34141 tgacggggat cagaaagtca aacaggttac atttgattca gcaaatcaac gcatgattat
    34201 tggcgatgat gtcaaaattt acactgtaaa cggtaatcaa atcgtcgtag atgatatgga
    34261 tagagaccca tcggaccaaa tcgttttaac taaataagac tatgcaagta gaaagggctg
    34321 gatttttcag cctttttact tttacctaga aaataatcat aaatacttgc cttctaccga
    34381 atacctgcgt tatactagta tcaattactt gtttttagca ttcaaactat caaggagggg
    34441 atatgaaata taggaaattt caattattga tgtccaagta tggctttagt ctttcgatta
    34501 tgctacttga actttgtctt gtttttggtc tctttcttta tttaggacgc atggctccca
    34561 ttttatggat tactgtcctc attctactga gtatcatcac aatcatttcg atagtcaacc
    34621 gtaatacgac tcctgagaat aaggtaacct ggttgttagt agcctttgtg ccagtatttg
    34681 gtcccttgct ctatctgatg tttggtgaaa ggcgattgtc caaaaaagaa atcaaacaac
    34741 tgaagaagct aggctctatg catttccaag aagcaaatag ccagctacta aaagagaaat
    34801 taaaagaaag tgacaaggca gcttatggag tcatcaagtc cttattgagt atggatacca
    34861 atgctgacat ctatgatcaa actgcctcta cattttttcc taacggagaa gctatgtgga
    34921 aaaagatggt agaagatctt aaaaaggctg agaaatttat tttcttggaa tattacatta
    34981 tagaagaagg tttgatgtgg aatcgcatac tagatatact agagcaaaag gtagctcagg
    35041 gtgtagaggt taagatgctc tatgatgata tcggctgtat ggctacttta acaggagatt
    35101 atgcacatcg acttcgtcag ctgggcatcg aggcccataa attcaataaa gttattcctc
    35161 gtttgacagt ggcttataat aacagagatc atagaaaaat attgattgtt gatggtcaga
    35221 tagcctatac tggtggggtc aatctggcag atgagtacat taaccacgtc gagagatttg
    35281 gttattggaa ggatagtgga attcgcttag acggactagc agtaaaagct ctgacacgct
    35341 tatttttgac cacttggtac attaatcgag gagaaattag tgattttgat caatatcatt
    35401 tagaaaatca ttctatcccg agtgacggtt taaccattcc atacggaagt ggacccaagc
    35461 caatttttcg agcgcaggta gggaaaaaag tttatcagag tttaatcaat caagcaacag
    35521 aatcggtcta tattacgaca ccttatttga ttatagatta tgatttaaca gagacaatca
    35581 aaaatgcagc tatgagaggg gtcgatgttc gaattatcac cccttacata ccagataaga
    35641 agttcattca gttagtcacg agaggagctt atcccgacct tctttctgct ggtgttcgga
    35701 tttatgagta tagtccaggt tttattcata gtaagcagat gttggtagac gaagattttg
    35761 cggtggtggg gacaatcaat ctcgactacc ggagcttggt acaccattat gaaaatgcag
    35821 tcttactcta taaaactcct tctataaggg aaatcgcccg agattttcga aatatatttg
    35881 cagattctca ggaagtctat cctcattcta tcaaaacgag ctggtatcaa aagcttgtaa
    35941 aagaaatcgc ccagctattc gcccctatct tataagaaat ctagaactta ggatacaaac
    36001 cagacctatt tttcttgtct tttacgaata gaaagaaaga ggaggggaat tcatgctaac
    36061 taaggaagaa gtgaatgata tgattgagtt caaacggaca catttacacc gctttcggga
    36121 aattgaaacc tttgtgaaac tatgcaaaaa atcactcaaa cagccatcgc aagctgacaa
    36181 tcctaggact ttttgatata ctaagacaga taaattgaac aggagaaacg atatgagtgt
    36241 gtatggtaga gtagaagaag ttcataagga gaatcgtgaa cctctagaat accaaatcga
    36301 acaagaatcg catcatcgtg aatcaagtcg tcttcctttg gtgaaaattt tactatggag
    36361 tacgcttgta acggggataa ctctaggagt accgctatta ctcgacttaa tgagtgcaca
    36421 agaagtgcag gatttttatg caggttgggc gcttcatcag acagggaaga tttacagcga
    36481 ctattatgga agtcaaggtt tgctttatta tttgctgact tacgtgagtc agggcggatt
    36541 tttctttgcc atctttgagt ggttagcctt ggtagcagga ggatttttcc tttttagatc
    36601 agcggacacc ttgacagagc aaggagacca agctggacat ctggtgacta ttttttacat
    36661 gctagttaca ggtcttgctt ttggtggagg ctatgcgact cttttagcgc ttcctttctt
    36721 attcgcagcc tttagtttag ttgcggctta cctaagcaat ccaagccatg ataagggatt
    36781 tgtacggatt gggctagctt tggcaggcgg atttttcttt gctcccttat catcgctcct
    36841 gtttattgct gtagtgagtt taggcttgtt ggtctttaac cttgggcata gacgttttgt
    36901 gcatgggttt tatcagtttc ttgcagtggc tttaggtttt tcacttgtct tttatccaac
    36961 tgcctactat agtgctgcaa caggaagttt tggggatgcg attagtggta ttcgttatcc
    37021 tattgacagt attcgctttg attttacttc taaaatttta gagaatatgt ttttttatgg
    37081 cttgttgtcc cttggtttgg gatttgtggt ttgtatcttt ttaggtctct ttcaatccaa
    37141 accctttaaa ctatacgtca tttcggttcc tgcaagtctt gtggtaattt taggtttgat
    37201 tttacttttc ttttcacaag agcctctgca cgcttcttat ttgatggtcg tcttccctgt
    37261 tttcctactt ttattggtaa ccaatattaa gagtcaacag agggggcgta gtgctagaag
    37321 aagccgaaga gaaacgccag ttagcctatg gagtcgtttc ttcaaaggaa atctatatct
    37381 gctagttttt gggtttgtct atcttttgtc tgttcctttt ttgatgaagt ttgtccttta
    37441 tccagtacct tatcaagaac gtaatcgtct tgctgatttg gtaaaagagg agacaaatac
    37501 ggaagatgct atctatgcat gggatgatac tgcgactctt tatcgtaaga gtgagcgctt
    37561 gtcgccatcg gcgattttgt ccccgttgca ctatacagca actgaagaaa atcgtaataa
    37621 gttacttaat gacttgaaag aaaaacaacc taaggtgatt gtggtaaatg acaaggtggt
    37681 agtctggtct gaagtggaaa cactcttaaa agaaaattac caacaagtaa agactgatta
    37741 ctcagagttt aaagtctata aaattaaata accaaatcaa tatcttgtgt atttttaaaa
    37801 attttaggat ttttaacaca agatattgat ttttcttttt agagtggtat aatacttttt
    37861 agaaagaaca ttttagaaaa gagcatgcat atgattgcac tagaagaaaa aattacaatt
    37921 ttgccaactc tcttcgtcga gaaacgagat gggagacgtg ttgtatttga tgtggacaag
    37981 attgacaagg ctctccacaa ggcggctgac aaggttatgg atgtgacacc cttggttgaa
    38041 aaacgcctca atgatctgac tgagcgaatt attacagaaa ttcatagtcg ctttccacag
    38101 ggaattaaga tttacgaaat tcaaaatatc gtagaacatg aactccttga agccaaagaa
    38161 tatgcgctgg ctgaggagta tattacttat cggacacaga gggattttga gcgctcaaaa
    38221 gcgacggata tcaactttag tattcataaa cttctcaaca aagaccagac agttgtcaat
    38281 gaaaatgcta ataaagacag tgatgtcttt aacactcagc gtgatttgac agcagggatt
    38341 gttgggaaat caatcggact gcaaatgctt cctaagcacg tagccaatgc ccaccaaaag
    38401 ggggatatcc actatcacga tttggactac agtccctata cccctatgac caactgctgt
    38461 ttgattgatt ttaagggtat gttggaaaat ggttttaaga ttggaaatgc agaggtagag
    38521 agtcccaagt ctatccagac tgcgacagca cagatttctc aaatcattgc caacgttgct
    38581 tctagccagt acggtggctg ttcagctgac cgtatcgatg aaattttggc gccttatgca
    38641 gagaagaact atcaaaaaca tctcaaagat gcagaagagt gggtattgcc tgaaaaacgg
    38701 gaagattacg cttggaagaa aacgcaaaag gacatctacg atgccatgca atctcttgag
    38761 tatgaaatca atactctctt cacttcaaat ggacaaacac cttttacttc gttaggtttt
    38821 ggtctgggaa ccagtcgttt tgaacgagaa attcaaaaag ctattttaaa cattcgcatc
    38881 aagggtcttg gttcagaaca ccgtacggct atctttccta aacttatttt cactctgaaa
    38941 agaggcctta atttagagga aggaactccc aactacgaca tcaagcagtt ggctcttgag
    39001 tgtgcaacca aacggatgta tccagatgtt ttatcttacg ataagattgt tgacttgaca
    39061 ggatccttca aggtgcctat gggttgccgt tctttcctcc aaggatggaa ggatgaaaat
    39121 ggtgtagaag tcaattcagg tcgcatgaat ctgggtgttg tgacggttaa tctgcctcgt
    39181 attgctcttg agtctgaagg tgatatgaat aagttctggg aaatcttcaa cgagcgaatg
    39241 aatatcgcag aagatgctct tgtttaccgt gtcgaacgca ctaaagaggc gacaccagcg
    39301 aatgctccta ttctttatca gtacggtgct tttggccatc gtctaggtaa agaagaaagt
    39361 gttgaccagc tctttaagaa tcgtcgtgcg accgtttcgc tgggctatat cggcttgtat
    39421 gaagtagcga cagttttctt tggtaacagc tgggaaagta atccagatgc taaggaattc
    39481 acgctagaca tcattcacga tatgaaacgc cgtgtagaag agtggtcaga ccaatatggc
    39541 taccatttct ctatctactc aacaccatcc gaaagtctga cagaccgttt ctgccgacta
    39601 gatatagaca agtttggctc tattcctgat atcacagaca aggaatacta caccaactct
    39661 ttccactacg atgttcgtaa aaatccaaca ccgtttgaaa aattggactt tgagaaagtc
    39721 tatccagaag caggtgcgtc aggtggtttc atccattatt gtgagtatcc agtccttcag
    39781 caaaatccaa aggccttgga agctgtctgg gattatgctt atgaccgtgt aggctatcta
    39841 ggcaccaata ctccgattga ccgttgctac aagtgtgact ttgaagggga ttttgaacca
    39901 actgagagag ggtttgcttg tccaaactgt ggcaatagcg atcctaaaac agtagacgtt
    39961 gttaaacgga cttgtggcta tcttggcaat cctcaagcga gaccgatggt taacgggcgt
    40021 cacaaggaaa tcgctgcgcg tgtcaaacat atgaatggtt caacgattaa aatagctggg
    40081 catcaagtaa caaattagaa agaaatgaaa tgggaaaata tcaactagac gataaggggc
    40141 gcgcacaagt gacccgttat cacgagaaat actctaaagg tggagctggt aagaaagaac
    40201 gcttgcttag cttcagagaa caatttttaa acaagaacaa gaaaaaataa aagtgagagc
    40261 cagctctcgc ttttctcata gtgggaggta gggatggaat tacgcagacc aagattagcg
    40321 gataagaaag ctgttttaga tatgatgaca gagtttgaaa agagccaatc agcccatgat
    40381 ggaggatttt gggatacaga gaactttgtg tatgaagagt ggttggaaag caatcaggaa
    40441 caggaaatgg ggattaatct gcctgaagga tgggttcctg caattcagtt agtggctttt
    40501 tctgagaaag gtcaagcagt tggatttctt aatctccggt tgcgcctcag taactttcta
    40561 ctagaagaag gtgggcacat tggctactcc atccgtccat ctgaaagagg caagggttat
    40621 gcaaaagaga ctctccgtca gggcttgcaa gttgctaagg aaaagaacat caagaaagct
    40681 ctggtgacct gtagcgtgaa taatcctgct agcagagcag tcattctagc aaatggtgga
    40741 atatttgagg atgctcgcaa tggagtcgag cgttattgga tagaggtagc gaatgaataa
    40801 tccaaaacca caagaatgga aaagcgagga acttagtcaa ggtcgtatca ttgactacaa
    40861 ggcctttaac tttgttgatg gcgaaggcgt gcgtaactct ctctatgtag caggctgtat
    40921 gtttcactgc gagggatgtt ataatgttgc gacttggtct tttaatgctg gcattcccta
    40981 tacagcagaa ttagaagagc agatcatggc agatcttgct cagccctatg ttcaaggttt
    41041 gactttgttg ggaggggagc cttttctcaa tactggcatt ctcttgccac ttgttaagcg
    41101 gattcggaaa gaattgtcag acaaggacat ctggtcctgg accggctaca cttgggaaga
    41161 aatgatgttg gaaactccag ataaactgga actcttgtca ctgattgata ttcttgtcga
    41221 tggaagatat gatcgaacta agagaaatct tatgctccag tttcgaggtt cgtctaacca
    41281 acgaattatc gatgtgcaaa aatcgctcaa aagtgggcaa gtagtgattt gggacaagct
    41341 caatgacgga aaagaaagct atgaacaggt gaagagagaa tgaagaaaaa agacttagta
    41401 gaccaactag tctcagagat cgagacgggg aaagtcagga cactgggaat atacggtcat
    41461 ggagcttcag gtaaatcaac ccttgcacag gaattgtccc aagctttaga ttctactaca
    41521 gtaaatttgc tagaaacaga tccctatatc acctccggac gctatttggt agtacccaag
    41581 gacgcgccga atcaaaaggt gacagccagt ctgccagtgg cgtatgaact ggagagtttg
    41641 cagagagata tccttgcctt gcaggcgggt atggatgtct tgacaattga agaaccttgg
    41701 aaggctagtg aagtcttgtc tggagccaaa ccaattttga ttgtcgaagg gatgtctgtt
    41761 ggttttctac ccaaggaact ctttgaaaaa accatctgtt tctacacgga tgaggagacc
    41821 gaattaaagc gacgctttgc tagagatacg actgtgagaa atcgcgatgc atcctttata
    41881 ttggctagcc atcagatgag acgggagcag tatctgcgat actataaaga aactgagtct
    41941 aaggcggata tcttagtgga ccaatcagaa gataaatttg atgtcaagag gacttaactt
    42001 atgtatagaa gaaaatccct aattttagaa agaaaaagta ggaaaacagt ttcatcccaa
    42061 aaaaacgaaa aaacctaaca aatcccttgc aatcgcaggg gatttgtgtt attctattat
    42121 ggtgctgtaa attacagcct tagttttgat gcaagaggtt gcgacacgct cggttgcatt
    42181 gccacgcaac accgcgtcgg ttttcttgtg gagctagcct attatcttaa atagacgaaa
    42241 aggagaaaaa gatggcaaac aaaaaaatcc gtatccgttt gaaagcttac gaacaccgta
    42301 cgcttgacac agcggctgca aaaatcgtag aatcagctac tcgtacaggt gcacaagttg
    42361 cgggtccaat cccacttcca actgaacgta gcctctacac aatcattcgt gcgactcaca
    42421 aatacaaaga ctctcgcgaa caatttgaaa tgcgtacaca caaacgtttg atcgatatcg
    42481 ttaacccaac tcaaaaaaca gttgatgcct tgatgaaatt ggatcttcca agtggtgtaa
    42541 acgtagaaat caaactttaa tctaaaatat aaaagagcag aggctggtgt ttcaatctaa
    42601 ttgaacacgg gctaaactcg gtgtgaaaaa gataaacttc ctagtgtctg ctagacactg
    42661 cgtcagtttc ctattttcac gttgagtttg acgccctttg tatcttagac ttgagcataa
    42721 aaaacgctcg ttaaaaactt tttgaataaa aatatagaaa aggaactatt ttctcatgac
    42781 aaaaggaatc ttagggaaaa aagtgggaat gactcaaatc ttcactgaag ctggcgaatt
    42841 gatccctgta acagttattg aagcaactcc aaacgttgtt cttcaagtta aaactgttga
    42901 aacagacgga tacaacgcta tccaagttgg tttcgatgac aaacgcgaag tattgagcaa
    42961 caaacctgct aaaggacatg tagcgaaagc taacacggct cctaagcgct tcattcgtga
    43021 attcaaaaac gttgaaggct tggaagttgg tgctgaaatt acagttgaaa cattcgcagc
    43081 tggagacgtt gttgacgtaa ctggtacttc taaaggtaaa ggtttccaag gtgttatcaa
    43141 acgccacgga caatcacgtg gaccaatggc tcacggttct cgttaccacc gtcgtccagg
    43201 ttctatggga cctgttgcac ctaaccgcgt attcaaaggt aaaaaccttg caggacgtat
    43261 gggtggtgac cgcgtaacaa ttcaaaatct tgaagttgta caagttgttc cagaaaagaa
    43321 cgttatcctt atcaaaggta acgtaccagg tgctaagaaa tctcttatca ctatcaaatc
    43381 agcagttaaa gctggtaaat aataaagaaa ggggaaatca gtcacaatgg caaacgtaac
    43441 attatttgac caaactggta aagaagctgg ccaagttgtt cttagcgatg cagtatttgg
    43501 tatcgaacca aatgaatcag ttgtgtttga tgtaatcatc agccaacgcg caagccttcg
    43561 tcaaggaaca cacgctgtta aaaaccgctc tgcagtatca ggtggtggac gcaaaccatg
    43621 gcgtcaaaaa ggaactggac gtgctcgtca aggttctatc cgctcaccac aatggcgtgg
    43681 tggtggtgtt gtcttcggac caactccacg ttcatacggc tacaaacttc cacaaaaagt
    43741 tcgtcgccta gctcttaaat cagtttactc tgaaaaagtt gctgaaaaca aattcgtagc
    43801 tgtagacgct ctttcattta cagctccaaa aactgctgaa tttgcaaaag ttcttgcagc
    43861 attgagcatc gattctaaag ttcttgttat ccttgaagaa ggaaatgaat tcgcagctct
    43921 ttcagctcgt aaccttccaa acgtgaaagt tgcaactgct acaactgcaa gtgttcttga
    43981 catcgcaaat agcgacaaac ttcttgtcac acaagcagct atctctaaaa tcgaggaggt
    44041 tcttgcataa tgaatttgta tgatgttatc aaaaaacctg tcatcactga aagctcaatg
    44101 gctcaacttg aagcaggaaa atatgtattt gaagttgaca ctcgtgcaca caaacttttg
    44161 atcaagcaag ctgttgaagc tgctttcgaa ggtgttaaag ttgccaatgt taacacaatc
    44221 aacgtaaaac caaaagctaa acgtgttgga cgttacactg gttttactaa caaaactaaa
    44281 aaagctatca tcacacttac agctgattct aaagcaatcg agttgtttgc tgctgaagct
    44341 gaataatcta aggaggaaat atcgtgggaa ttcgtgttta taaaccaaca acaaacggtc
    44401 gccgtaatat gacttctttg gatttcgctg aaatcacaac aagcactcct gaaaaatcat
    44461 tgcttgttgc attgaagagc aaggctggtc gtaacaacaa cggtcgtatc acagttcgtc
    44521 accaaggtgg tggacacaaa cgtttctacc gtttggttga cttcaaacgt aataaagaca
    44581 acgttgaagc agttgttaaa acaatcgagt acgatccaaa ccgttctgca aacatcgctc
    44641 ttgtacacta cactgacggt gtgaaagcat acatcatcgc tccaaaaggt cttgaagtag
    44701 gtcaacgtat cgtttcaggt ccagaagcag atatcaaagt cggaaacgct cttccacttg
    44761 ctaacatccc agttggtact ttgattcaca acatcgagtt gaaaccaggt cgtggtggtg
    44821 aattggtacg tgctgctggt gcatctgctc aagtattggg ttctgaaggt aaatacgttc
    44881 ttgttcgtct tcaatcaggt gaagttcgta tgattcttgg aacttgccgt gctacagttg
    44941 gtgttgtcgg aaacgaacaa catggacttg taaaccttgg taaagcagga cgtagccgtt
    45001 ggaaaggtat ccgcccaaca gttcgtggtt ctgtaatgaa ccctaacgat cacccacacg
    45061 gtggtggtga aggtaaagca ccagttggtc gtaaagcacc atctactcca tggggcaaac
    45121 ctgctcttgg tcttaaaact cgtaacaaga aagcgaaatc tgacaaactt atcgttcgtc
    45181 gtcgcaacga gaaataatat taaactagtc gcttaagcaa ctagtaaatc cgccagctcg
    45241 gtagcgctcc ataggagtgc aagccgctgt ggtacaacat ttaaaggaga aaatataaaa
    45301 atgggacgca gtcttaaaaa aggacctttc gtcgatgagc atttgatgaa aaaagttgaa
    45361 gctcaagcta acgacgaaaa gaaaaaagtt attaaaactt ggtcacgtcg ttcaacgatc
    45421 ttcccaagtt tcattggtta cactattgca gtttatgacg gacgtaaaca cgtacctgtt
    45481 tacatccaag aagacatggt aggccacaaa cttggtgaat ttgcaccaac tcgtacttac
    45541 aaaggtcacg ctgcagacga caagaaaaca cgtagaaaat aaggagaaca taaatggcag
    45601 aaattacttc agctaaagca atggctcgta cagtacgtgt ttcacctcgt aaatcacgtc
    45661 ttgttcttga taacatccgt ggtaaaagcg tagccgatgc aatcgcaatc ttgacattca
    45721 ctccaaacaa agctgctgaa atcatcttga aagttttgaa ctcagctgta gctaacgctg
    45781 aaaacaactt tggtttggat aaagctaact tggtagtatc tgaagcattc gcaaacgaag
    45841 gaccaactat gaaacgtttc cgtccacgtg cgaaaggttc agcttcacca atcaacaaac
    45901 gtacagctca catcactgta gctgttgcag aaaaataagg aggtaaaatc gtgggtcaaa
    45961 aagtacatcc aattggtatg cgtgtcggca tcatccgtga ttgggatgcc aaatggtatg
    46021 ctgaaaaaga atacgcggat taccttcatg aagatcttgc aatccgtaaa ttcgttcaaa
    46081 aagaacttgc tgacgcagca gtttcaacta ttgaaatcga acgcgcagta aacaaagtta
    46141 acgtttcact tcacactgct aaaccaggta tggttatcgg taaaggtggt gctaacgttg
    46201 atgcactccg tgcaaaactt aacaaattga ctggaaaaca agtacacatc aacatcatcg
    46261 aaatcaaaca acctgatttg gatgctcacc ttgtaggtga aggaattgct cgtcaattgg
    46321 agcaacgtgt tgctttccgt cgtgcacaaa aacaagcaat ccaacgtgca atgcgtgctg
    46381 gagctaaagg aatcaaaact caagtatcag gtcgtttgaa cggtgcagat atcgcccgtg
    46441 ctgaaggata ctctgaagga actgttccgc ttcacacact tcgtgcagat atcgattacg
    46501 cttgggaaga agcagatact acatacggta aacttggtgt taaagtatgg atctaccgtg
    46561 gtgaagttct tccagctcgt aaaaacacta aaggaggtaa ataaccaatg ttagtaccta
    46621 aacgtgttaa acaccgtcgt gaattccgtg gaaaaatgcg cggtgaagca aaaggtggaa
    46681 aagaagtagc attcggtgaa tacggtcttc aagctacaac tagccactgg atcactaacc
    46741 gccaaatcga agctgctcgt atcgccatga ctcgttacat gaaacgtggt ggtaaagttt
    46801 ggattaaaat cttcccacac aaatcataca ctgctaaagc tatcggtgtg cgtatgggat
    46861 ctggtaaagg ggcacctgaa ggttgggtag caccagttaa acgtggtaaa gtgatgttcg
    46921 aaatcgctgg tgtatctgaa gagattgcac gtgaagcgct tcgacttgct agccacaaat
    46981 tgccagttaa atgtaaattc gtaaaacgtg aagcagaata aggagaaggc atgaaactta
    47041 atgaagtaaa agaatttgtt aaagaacttc gtggtctttc tcaagaagaa ctcgcgaagc
    47101 gcgaaaacga attgaaaaaa gaattgtttg aacttcgttt ccaagctgct actggtcaat
    47161 tggaacaaac agctcgcttg aaagaagtta aaaaacaaat cgctcgtatc aaaacagttc
    47221 aatctgaagc gaaataatag actagggaag gagaaatttc aatggaacgc aataatcgta
    47281 aagttcttgt tggacgtgtt gtatctgaca aaatggacaa gacaatcaca gttgtagttg
    47341 aaacaaaacg taaccaccca gtctatggta aacgtattaa ctactctaaa aaatacaaag
    47401 ctcatgatga aaacaatgtt gccaaagaag gtgatatcgt acgtatcatg gaaactcgcc
    47461 cgctttcagc tacaaaacgt ttccgtcttg tagaagttgt tgaagaagcg gtcatcatct
    47521 aatcaaacct gaaaggagaa aactgaaatg attcaaacag aaactcgttt gaaagtcgca
    47581 gacaacagcg gtgctcgcga aatcttgact atcaaagttc ttggtggttc aggacgtaaa
    47641 tttgcaaaca tcggtgatgt tatcgtggca tctgtaaaac aagctactcc tggtggtgcg
    47701 gttaaaaaag gtgacgttgt taaagcagtt atcgttcgta ctaaatcagg tgctcgtcgt
    47761 gctgatggtt catacatcaa atttgacgaa aacgcagcag ttatcatccg tgaagacaaa
    47821 actcctcgcg gaacacgtat ctttggccca gttgcacgtg aattgcgtga aggtggtttc
    47881 atgaagatcg tgtcacttgc tccagaagta ctttaatttt taggaacaaa ctagtcccct
    47941 agcttcaagc tagggtgccc ttatgggcgt aagaaaaatc aaggagaaac ctaatgtttg
    48001 taaaaaaagg cgacaaagtt cgcgtaatcg ctggtaaaga taagggaaca gaagctgttg
    48061 tccttactgc ccttccaaaa gtaaacaaag ttatcgttga aggtgttaac attgttaaga
    48121 aacaccaacg tccaactaac gagcttcctc aaggtggtat catcgagaaa gaagcagcta
    48181 tccacgtatc aaatgttcaa gttttggaca aaaatggtgt agctggtcgt gttggttaca
    48241 aatttgtaga cggtaaaaaa gttcgctaca acaaaaaatc aggcgaagtg cttgattaat
    48301 cacgaaggaa aggagaagta taatggcaaa tcgtttaaaa gaaaaatatc ttaatgaagt
    48361 agttcctgct ttgacagaac aattcaacta ctcatcagtg atggctgtgc ctaaagtaga
    48421 taagattgtt ttgaacatgg gtgttggtga agctgtatca aacgctaaaa gccttgaaaa
    48481 agctgctgaa gaattggcac ttatctcagg tcaaaaacca cttatcacta aagctaaaaa
    48541 atcaatcgcc ggcttccgtc ttcgtgaagg tgttgcgatc ggtgcaaaag ttacccttcg
    48601 tggtgaacgt atgtacgaat tcttggataa attggtatca gtttcacttc cacgtgtacg
    48661 tgacttccac ggtgtcccaa caaaatcatt tgatggacgc gggaactaca cacttggtgt
    48721 gaaagaacaa ttaatcttcc cagaaatcaa cttcgatgac gttgacaaaa ctcgtggtct
    48781 tgacatcgtt atcgtaacaa ctgctaacac tgacgaagag tcacgtgcat tgcttacagg
    48841 ccttggaatg ccttttgcaa aataatatag gaggtaaatc taatggctaa aaaatcaatg
    48901 gtagctagag aggctaaacg ccaaaaaatt gttgaccgtt atgctgaaaa acgtgctgca
    48961 ttaaaggcgg caggggacta cgaaggttta tctaaattac ctcgcaacgc ctcaccgact
    49021 cgtttacata atcgttgtag agttacgggg cgcccacatt cagtttaccg caaatttggt
    49081 ctgagtcgta tcgcttttcg cgaacttgcg cataaaggtc aaattcctgg tgtaacaaaa
    49141 gcatcttggt aatttaagat atcaagggtg tcaaaactcc aagtaaaaat aggaaacttg
    49201 acgaagaaac taaagtttct aggaaagttt atctttttca cacagagttt agcccgggtt
    49261 caattgggct tgccaatttg aacccgagct acagctttgg caaaaaagac caatttgctt
    49321 tggagcattg cttctgcatt aaattgtcta tttttgctcg tgctgttacg ctctttgtat
    49381 catgtattaa ctagcaagtg caacttgcaa actactagta agaggagaaa aacaaaatgg
    49441 ttatgactga cccaatcgca gacttcctaa ctcgtattcg taatgctaac caagctaaac
    49501 acgaagtact tgaagtacct gcatcaaaca tcaaaaaagg gattgctgaa atccttaaac
    49561 gcgaaggttt tgtaaaaaac gttgaaatca ttgaagatga caaacaaggc gtcatccgtg
    49621 tatttcttaa atacggatca aatggtgaga aagttatcac taacttgaaa cgtgtttcta
    49681 aaccaggact tcgtgtctac aaaaaacgtg aagaccttcc aaaagttctt aacggacttg
    49741 gaattgccat cctttcaact tctgaaggtt tgcttactga taaagaagca cgccaaaaga
    49801 atgttggtgg tgaggttatc gcttacgttt ggtaaaatca agatacaaag ctcgtaaaga
    49861 acaaagcaaa attaggaagt tggagaagtt tgtttacaaa caagccaact tatctatttt
    49921 gcacagttct tagagcgtgt tcagttcagc tcttgaacta aataagtatc tgaaccccgt
    49981 gaaaactggc cgttctggcc tgacaattta acaggagaaa ataaacatgt cacgtattgg
    50041 taataaagtt atcgtgttgc ctgctggtgt tgaactcgct aacaatgaca acgttgtaac
    50101 tgtaaaagga cctaaaggag aacttactcg tgagttctca aaagatattg aaatccgtgt
    50161 ggaaggtact gaaataactc ttcaccgtcc aaacgattca aaagaaatga aaactatcca
    50221 cggaactact cgtgcccttt tgaacaacat ggttgttggt gtatcagaag gattcaagaa
    50281 agaacttgaa atgcgtgggg ttggttaccg tgcacagctt caaggatcta aacttgtttt
    50341 ggctgttggt aaatctcatc cagacgaagt tgaagctcca gaaggaatta cttttgaact
    50401 tccaaaccca acaacaatcg ttgttagcgg aatttcaaaa gaagtagttg gtcaaacagc
    50461 tgcttacgta cgtagccttc gttcaccaga accatataaa ggtaaaggta tccgttacgt
    50521 tggtgaattc gttcgccgta aagaaggtaa aacaggtaaa taatgttgag tggttgatca
    50581 tcaaccacca acctattttc caactttgtg catagcacac gatttaaaac taaagaggtg
    50641 aaaactgtga tttcaaaacc agataaaaac aaactccgcc aaaaacgcca ccgtcgcgtt
    50701 cgcggaaaac tctctggaac tgctgatcgc ccacgtttga acgtattccg ttctaataca
    50761 ggcatctacg ctcaagtgat tgatgacgta gcgggtgtaa cgctcgcaag tgcttcaact
    50821 cttgataaag aagtttcaaa aggaactaaa actgaacaag ccgttgctgt cggtaaactc
    50881 gttgcagaac gtgcaaacgc taaaggtatt tcagaagtgg tgttcgaccg cggtggatat
    50941 ctatatcacg gacgtgtgaa agctttggct gatgcagctc gtgaaaacgg attgaaattc
    51001 taataggagg acactagaaa atggcattta aagacaatgc agttgaatta gaagaacgcg
    51061 tagttgctgt caaccgtgtt acaaaagttg ttaaaggtgg acgtcgtctt cgtttcgcag
    51121 ctcttgttgt tgttggtgac cacaatggtc gcgtaggatt tggtactggt aaagctcaag
    51181 aagttccaga agcaatccgt aaagcagtag atgatgctaa gaaaaacttg atcgaagttc
    51241 ctatggttgg aacaacaatc ccacacgaag ttctttcaga attcggtgga gctaaagtat
    51301 tgttgaaacc tgctgtagaa ggttctggag ttgccgctgg tggtgcagtt cgtgccgttg
    51361 tggaattggc aggtgtggca gatattacat ctaaatcact tggttctaac actccaatca
    51421 acattgttcg tgcaactgtt gaaggtttga aacaattgaa acgcgctgaa gaaattgctg
    51481 cccttcgtgg tatttcagtt tctgatttgg cataagaaag gggataaaat ggctcaaatt
    51541 aaaattactt tgactaagtc tccaatcgga cgcattccat cacaacgtaa aactgttgta
    51601 gcacttggac ttggcaaatt gaacagctct gttattaaag aagataacgc tgctatccgt
    51661 ggtatgatta cagcagtatc tcacttagta acagttgaag aagtaaacta atgaagtttt
    51721 aggggatgtg cactgtacca tcccctaaaa ctagatatag tcatctatga tgacatcgta
    51781 taggcgagtt gatgggggag acaacctttt ctcccttatc ggcgctagca ttttacaaaa
    51841 gaggagaaaa taaaaatgaa acttcatgaa ttgaaacctg cagaaggttc tcgtaaagta
    51901 cgtaaccgcg ttggtcgtgg tacttcatca ggtaacggta aaacatctgg tcgtggtcaa
    51961 aaaggtcaaa aagctcgtag cggtggcgga gttcgccttg gttttgaagg tggacaaact
    52021 ccattgttcc gtcgtcttcc aaaacgtgga ttcactaaca tcaacgctaa agaatacgca
    52081 attgtgaacc ttgaccaatt gaacgtcttt gaagatggtg ctgaagtaac tccagttgtt
    52141 cttatcgaag caggaattgt taaagctgaa aagtcaggta ttaaaattct tggtaacggt
    52201 gagttgacta agaaattgac tgtgaaagca gctaaattct ctaaatcagc tgaagaagct
    52261 atcactgcta aaggtggttc agtagaagtc atctaagaga ggtgacctat gttttttaaa
    52321 ttattaagag aagctcttaa agtcaagcag gttcgatcaa aaattttatt tacaattttt
    52381 atcgttttgg tctttcgtat cggaactagc attacagttc ctggtgtgaa tgccaatagc
    52441 ttgaatgctt taagtggatt atccttctta aacatgttga gcttggtgtc ggggaatgcc
    52501 ctaaaaaact tttcgatttt tgccctagga gttagtccct atatcaccgc ttctattgtt
    52561 gtccaactct tgcaaatgga tattttaccc aagtttgtag agtggggtaa acaaggggaa
    52621 gtaggtcgaa gaaaattgaa tcaagctact cgttatattg ctctagttct cgcttttgtg
    52681 caatctatcg ggattacagc tggttttaat accttggctg gagctcaatt gattaaaact
    52741 gctttaactc cacaagtttt tctgacgatt ggtatcatct taacagctgg tagtatgatt
    52801 gtcacttggt tgggtgagca aattacagat aagggatacg gaaacggtgt ttccatgatt
    52861 atctttgccg ggattgtttc ctcaattcca gagatgattc agggcatcta tgtggactac
    52921 tttgtgaacg tcccaagtag ccgtatcact tcatctatca ttttcgtaat cattttgatt
    52981 attactgtat tgttgattat ttactttaca acttatgttc aacaagcaga atacaaaatt
    53041 ccaatccaat atactaaggt tgcacaaggt gctccatcta gctcttacct tccgttaaag
    53101 gtaaaccctg ctggagttat ccctgttatc tttgccagtt cgattactgc agcgcctgcg
    53161 gctattcttc agtttttgag tgccacaggt catgattggg cttgggtaag ggtagcacaa
    53221 gagatgttgg caactacttc tccaactggt attgccatgt atgctttgtt gattattctc
    53281 tttacattct tctatacgtt tgtacagatt aatcctgaaa aagcagcaga gagcctacaa
    53341 aagagtggtg cctatatcca tggagttcgt cctggtaaag gtacagaaga atatatgtct
    53401 aaacttcttc gtcgtcttgc aactgttggt tccctcttcc ttggtgtgat ttccatttta
    53461 ccgattgcag ctaaagatgt atttggtctt tctgatgttg ttgcctttgg tggaacaagt
    53521 ctcttgatca ttatctctac aggtatcgaa ggaatcaagc aattggaagg ttacctattg
    53581 aaacgtaagt atgttggttt catggacaga acagaataaa agtatttact gaatcagtaa
    53641 atactgaggg agtggaggtt taaactctga catttgtaag agttggatct cccctcttct
    53701 attttgtttt taaatagggg tgaaaagact ttttgcttct atttaaaaat aaaataagga
    53761 gatcaaatca tgaatctttt gattatgggc ttacctggtg caggtaaggg aactcaagca
    53821 gcaaaaatcg tagaacaatt ccatgttgca catatctcaa caggtgatat gttccgcgct
    53881 gcaatggcaa atcaaactga aatgggtgtt cttgctaagt catatattga caagggtgaa
    53941 ttggttcctg acgaagttac aaatggaatc gtaaaagaac gcctttcaca agatgatatt
    54001 aaagaaacag gattcttatt ggatggttac ccacgtacaa ttgaacaagc tcatgccttg
    54061 gacaaaacat tggctgaact tggcattgaa ctagaaggtg ttatcaatat tgaagtgaac
    54121 cctgacagcc tcttggaacg tttgagtggc cgtatcatcc accgcgtaac tggagaaact
    54181 ttccacaagg tctttaaccc accagttgac tataaagaag aagattacta ccaacgtgaa
    54241 gatgataagc ctgagacagt aaaacgtcgt ttggatgtta atattgctca aggagaacca
    54301 atcattgctc actaccgtgc caaaggtttg gttcatgaca tcgaaggtaa tcaagatatc
    54361 aatgatgtct tctcagatat tgaaaaagta ttgacaaatt tgaaataaag cgtttttcac
    54421 acttgcaaaa atccgctaca aatgttatac tgagatagtc tgacttataa ttgttgtctc
    54481 tgtgtctaga ggcatcgaat cgaaatttat ggaggtgctt ttgcgtggca aaagacgatg
    54541 tgattgaagt tgaaggcaaa gtagttgata caatgccgaa tgcaatgttt acggttgaac
    54601 ttgaaaatgg acatcagatt ttagcaacag tttctggtaa aattcgtaaa aactatattc
    54661 gtattttagc gggagatcgt gttactgtcg aaatgagtcc atatgacttg acacgtggac
    54721 gtatcactta ccgctttaaa taatcgaaaa acttggaggg ataagaaatg aaagtaagac
    54781 catcggtcaa accaatttgc gaatactgta aagttattcg tcgtaatggt cgtgttatgg
    54841 taatttgccc agcaaatcca aaacacaaac aacgtcaagg ataagataga aaggagaaaa
    54901 catggctcgt attgctggag ttgatattcc aaatgacaaa cgcgtagtaa tctcattgac
    54961 ttatgtttat ggtatcggac ttgcaacatc taagaaaatt ttggctgctg ctggaatctc
    55021 agaagatgtt cgtgtacgtg atcttacatc agatcaagaa gatgctatcc gtcgtgaagt
    55081 ggatgcaatc aaagttgaag gtgaccttcg tcgtgaagta aacttgaaca tcaaacgttt
    55141 gatggaaatc ggttcatacc gtggtatccg tcaccgtcgt ggacttcctg tccgtggaca
    55201 aaacactaaa aacaacgctc gcactcgtaa aggtaaagct gttgcgattg ctggtaagaa
    55261 aaaataatat aggaggtaaa agtcttggct aaaccaacac gtaaacgtcg tgtgaaaaag
    55321 aatatcgaat ctggtattgc tcatattcac gctacattta ataacactat tgttatgatt
    55381 actgatgtgc atggtaatgc aattgcttgg tcatcagctg gtgctcttgg tttcaaaggt
    55441 tctcgtaaat ctacaccatt cgctgctcaa atggcttctg aagctgctgc taaatctgca
    55501 caagaacacg gtcttaaatc agttgaagtt actgtaaaag gtccaggttc tggtcgtgag
    55561 tcagctattc gtgcgcttgc tgccgctggt cttgaagtaa cagcaattcg tgatgtgact
    55621 ccagtgccac acaatggtgc tcgtcctcca aaacgtcgcc gtgtataatc atcgcattac
    55681 actgcttttc gtttaagagg gagtaactaa atgatcgagt ttgaaaaacc aaatataaca
    55741 aaaattgatg aaaataaaga ttatggcaag tttgtaatcg aaccacttga acgtggctac
    55801 ggtacaactc ttggtaactc tcttcgtcgt gtacttctag cttctctacc aggagcagct
    55861 gtgacatcta tcaacattga tggtgtgtta catgagtttg acacagttcc aggtgttcgt
    55921 gaagacgtga tgcaaatcat tctgaacatt aaaggaattg cagtgaaatc gtacgttgaa
    55981 gacgaaaaaa tcatcgaact ggatgttgaa ggtcctgctg aagtaacagc tggtgacatt
    56041 ttgacagata gcgatattga aattgtaaat ccagatcatt atctctttac aatcggtgaa
    56101 ggttcttctc taaaagcgac tatgactgtt aacagtggtc gtggatatgt acctgctgat
    56161 gaaaataaaa aggataatgc accagttgga acacttgctg tagattctat ttatacacca
    56221 gttacaaaag tcaactatca agtggaacct gctcgtgtag gtagcaatga tggtttcgac
    56281 aaattaaccc ttgaaatctt gacaaatgga acaattattc cagaagatgc tttagggctt
    56341 tcagcacgta ttttgacaga acatcttgat ttgtttacaa atcttactga gattgctaag
    56401 tcaactgaag tgatgaaaga agctgatact gaatctgacg accgtatttt agatcgtacg
    56461 attgaggaac tggacttgtc tgtgcgttca tacaactgtt taaaacgtgc cggtatcaat
    56521 actgtgcatg atttgacaga aaaatctgaa gcagagatga tgaaagtacg aaatcttgga
    56581 cgcaagagtt tggaagaagt gaaactcaaa ctcattgatt tgggtcttgg attaaaagat
    56641 aaataaagga ggaatacatg gcttaccgta aactaggacg cactagctca caacgtaaag
    56701 caatgcttcg cgatttgaca actgaccttt tgatcaacga atcaatcgtg acaactgaag
    56761 ctcgtgctaa agaaatccgt aaaactgttg aaaaaatgat tactctaggt aaacgtggtg
    56821 atttgcatgc acgtcgtcaa gcagctgctt tcgtacgtaa tgaaatcgca tctgaaaact
    56881 atgatgaagc aactgataag tacacttcta ctacagcact tcaaaaattg ttctcagaaa
    56941 tcgcacctcg ttatgctgaa cgtaacggtg gatacactcg tatccttaaa actgaaccac
    57001 gtcgtggtga tgcagcgcca atggcgatca tcgaattagt ataaaatcat caattttgtt
    57061 gagtgttatg atgatggagt cttgtgctct tagtctagct ctggtctacc gctaggattt
    57121 cggtcctagc gggaacactc atcataagtt gggatagtag acgcttgttt acgaaattgt
    57181 ttttttctta agaacaactt cgtaagcagg cgtttttgag tattttcgtt agaattatgc
    57241 tatactattt gaaaagaatc ctgtttaatg ttaaggtttc ttattttaag aagaattgga
    57301 gtttacttat gaaagcgatt ataactgttg ttggtaaaga taaatctgga attgttgcag
    57361 gtgtttctgg taaaattgca gaattaggat tgaatattga cgatatctct caaactgtat
    57421 tggatgaata ttttacgatg atggctgttg tatctagtga tgaaaagcaa gattttacct
    57481 atcttcgtaa tgaatttgaa gcttttgggc aaactttgaa tgtaaaaatc aatattcaga
    57541 gtgcagcgat tttcgaagct atgtataata tctaggaggt catcatggat attagacaag
    57601 ttactgaaac catcgccatg attgaggagc aaaacttcga tattagaacc attaccatgg
    57661 ggatttctct tttggactgt atcgatccaa atatcaatcg tgctgcggag aaaatctatc
    57721 aaaaaattac gacaaaggcg gctaatttag tagctgttgg tgatgaaatt gcggctgagt
    57781 tgggaattcc tatcgttaat aagcgtgtat cggtgacacc tatttctctg attggggcag
    57841 cgacagatgc gacggactac gtggttctgg caaaagcgct tgataaggct gcgaaagaga
    57901 ttggtgtgga ctttattggt ggtttttctg ccttagtaca aaaaggttat caaaagggag
    57961 atgagattct catcaattcc attcctcgcg ctttggctga gacggataag gtctgctcgt
    58021 cagtcaatat cggctcaacc aagtctggta ttaatatgac ggctgtggca gatatgggac
    58081 gaattatcaa ggaaacagca aatctttcag atatgggagt ggccaagttg gttgtattcg
    58141 ctaatgctgt tgaggacaat ccatttatgg cgggtgcctt tcatggtgtt ggggaagcag
    58201 atgttatcat caatgtcgga gtttctggtc ctggtgttgt gaaacgtgct ttggaaaaag
    58261 ttcgtggaca gagctttgat gtagtagccg aaacagttaa gaaaactgcc tttaaaatca
    58321 ctcgtatcgg tcaattggtt ggtcaaatgg ccagtgagag actgggtgtg gagtttggta
    58381 ttgtggactt gagtttggca ccaacccctg cggttggaga ctctgtggca cgtgtccttg
    58441 aggaaatggg gctagaaaca gttggcacgc atggaacgac ggctgccttg gccctcttga
    58501 acgaccaagt taaaaagggt ggagtgatgg cctgcaacca agtcggtggt ttatctggtg
    58561 cctttatccc tgtttctgag gatgaaggaa tgattgctgc agtgcaaaat ggctctctta
    58621 atttagaaaa actagaagct atgacggcta tctgttctgt tggattggat atgattgcca
    58681 tcccagaaga tacgcctgct gaaaccattg cggctatgat tgcggatgaa gcagcaatcg
    58741 gtgttatcaa catgaaaaca acagctgttc gtatcattcc caaaggaaga gaaggcgata
    58801 tgattgagtt tggtggtcta ttaggaactg cacccgttat gaaggttaat ggggcttcat
    58861 ctgtcgactt catctctcgc ggtggaca
//
LOCUS       DAVLYV010000005        58249 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLYV010000005 DAVLYV010000000
VERSION     DAVLYV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.005"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            434..1909
                     /locus_tag="VYO70_000276"
     CDS             434..1909
                     /locus_tag="VYO70_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1712572.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1875..2464)
                     /locus_tag="VYO70_000277"
                     /pseudo
     CDS             complement(1875..2464)
                     /locus_tag="VYO70_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2845..3705
                     /gene="speE"
                     /locus_tag="VYO70_000278"
     CDS             2845..3705
                     /gene="speE"
                     /locus_tag="VYO70_000278"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1712573.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            3702..4961
                     /locus_tag="VYO70_000279"
     CDS             3702..4961
                     /locus_tag="VYO70_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1712574.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVKKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4961..6088
                     /gene="nspC"
                     /locus_tag="VYO70_000280"
     CDS             4961..6088
                     /gene="nspC"
                     /locus_tag="VYO70_000280"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1712575.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKEVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6085..7170
                     /gene="aguA"
                     /locus_tag="VYO70_000281"
     CDS             6085..7170
                     /gene="aguA"
                     /locus_tag="VYO70_000281"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1712576.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7180..8055
                     /gene="aguB"
                     /locus_tag="VYO70_000282"
     CDS             7180..8055
                     /gene="aguB"
                     /locus_tag="VYO70_000282"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1712577.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8236..9045)
                     /locus_tag="VYO70_000283"
     CDS             complement(8236..9045)
                     /locus_tag="VYO70_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1712578.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLELDLQNEYIIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            9317..9538
                     /locus_tag="VYO70_000284"
     CDS             9317..9538
                     /locus_tag="VYO70_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1712579.1"
                     /translation="MILMTKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLL
                     VHGFCPRGTDDLGYIGGGNHLCKGSAARF"
     gene            9592..10263
                     /locus_tag="VYO70_000285"
     CDS             9592..10263
                     /locus_tag="VYO70_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1712580.1"
                     /translation="MKKRAIQILLVLSLIFYKSTWFWRLFNYLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISPPS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVIMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(10315..10512)
                     /locus_tag="VYO70_000286"
                     /pseudo
     CDS             complement(10315..10512)
                     /locus_tag="VYO70_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            10505..11413
                     /locus_tag="VYO70_000287"
     CDS             10505..11413
                     /locus_tag="VYO70_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1712581.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLREGHPLAQKDELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11410..11871
                     /locus_tag="VYO70_000288"
     CDS             11410..11871
                     /locus_tag="VYO70_000288"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1712582.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            11861..12748
                     /locus_tag="VYO70_000289"
     CDS             11861..12748
                     /locus_tag="VYO70_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1712583.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLALAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            12751..14634
                     /gene="cbpE"
                     /locus_tag="VYO70_000290"
                     /gene_synonym="pce"
     CDS             12751..14634
                     /gene="cbpE"
                     /locus_tag="VYO70_000290"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1712584.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWRQGINTTYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRQDGLVNISTSYKPIPS
                     FQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNNRWFY
                     LTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            14714..15844
                     /locus_tag="VYO70_000291"
     CDS             14714..15844
                     /locus_tag="VYO70_000291"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1712585.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            15854..17116
                     /locus_tag="VYO70_000292"
     CDS             15854..17116
                     /locus_tag="VYO70_000292"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1712586.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            17120..17911
                     /gene="proC"
                     /locus_tag="VYO70_000293"
     CDS             17120..17911
                     /gene="proC"
                     /locus_tag="VYO70_000293"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1712587.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMDAVHQAYKRTQEL"
     gene            17992..18630
                     /locus_tag="VYO70_000294"
     CDS             17992..18630
                     /locus_tag="VYO70_000294"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1712588.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLEKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            18627..19517
                     /locus_tag="VYO70_000295"
     CDS             18627..19517
                     /locus_tag="VYO70_000295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1712589.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLTKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            19557..19874
                     /gene="yabA"
                     /locus_tag="VYO70_000296"
     CDS             19557..19874
                     /gene="yabA"
                     /locus_tag="VYO70_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1712590.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            19877..20746
                     /gene="rsmI"
                     /locus_tag="VYO70_000297"
     CDS             19877..20746
                     /gene="rsmI"
                     /locus_tag="VYO70_000297"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1712591.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFDS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASKGVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            20973..21491
                     /locus_tag="VYO70_000298"
     CDS             20973..21491
                     /locus_tag="VYO70_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1712592.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            21600..22247
                     /locus_tag="VYO70_000299"
     CDS             21600..22247
                     /locus_tag="VYO70_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1712593.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(22236..23040)
                     /locus_tag="VYO70_000300"
     CDS             complement(join(22236..22670,22672..23040))
                     /locus_tag="VYO70_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1712594.1"
                     /translation="MNDEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLHYVREYRTYEEIAADFGIHESNLLRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATKVKINRPKKELANDSGKKKCHAMKSQAIVTSQGRIVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLIAEDKAYNHA
                     LSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            23050..23290
                     /locus_tag="VYO70_000301"
                     /pseudo
     CDS             23050..23290
                     /locus_tag="VYO70_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
     gene            23535..24869
                     /gene="trmFO"
                     /locus_tag="VYO70_000302"
     CDS             23535..24869
                     /gene="trmFO"
                     /locus_tag="VYO70_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1712595.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGAGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            24953..25690
                     /locus_tag="VYO70_000303"
     CDS             24953..25690
                     /locus_tag="VYO70_000303"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1712596.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQPGNIKRVVFGENIGTTVSNNIEEKE"
     gene            25699..26256
                     /gene="frr"
                     /locus_tag="VYO70_000304"
     CDS             25699..26256
                     /gene="frr"
                     /locus_tag="VYO70_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1712597.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKREKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            26316..27170
                     /gene="cvfB"
                     /locus_tag="VYO70_000305"
     CDS             26316..27170
                     /gene="cvfB"
                     /locus_tag="VYO70_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1712598.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            27179..27394
                     /locus_tag="VYO70_000306"
     CDS             27179..27394
                     /locus_tag="VYO70_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1712599.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDAIWQEYLEH"
     gene            27480..28448
                     /locus_tag="VYO70_000307"
     CDS             27480..28448
                     /locus_tag="VYO70_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1712600.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            complement(28426..28605)
                     /locus_tag="VYO70_000308"
     CDS             complement(28426..28605)
                     /locus_tag="VYO70_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712601.1"
                     /translation="MTQEDALIVISHIKVLSIVPNRCLRPLDKTFSLYNWIFLSQKYI
                     LLQANFLKISRVQLQ"
     gene            28641..29141
                     /locus_tag="VYO70_000309"
     CDS             28641..29141
                     /locus_tag="VYO70_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1712602.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            29144..29404
                     /locus_tag="VYO70_000310"
                     /pseudo
     CDS             29144..29404
                     /locus_tag="VYO70_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(29771..30097)
                     /locus_tag="VYO70_000311"
     CDS             complement(29771..30097)
                     /locus_tag="VYO70_000311"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712603.1"
                     /translation="MVDIAVDQGGLIETADRVTTHDEPVYEKHGVLHYAVANIPGAVA
                     RTSTIALTNVTLPYIEALAGKGFAQAISEDEGLRQGVTTYQGYLTSLPVAQGLNRDYT
                     DINDLV"
     gene            complement(30043..30369)
                     /locus_tag="VYO70_000312"
     CDS             complement(30043..30369)
                     /locus_tag="VYO70_000312"
                     /inference="COORDINATES: protein motif:HMM:NF013430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1712604.1"
                     /translation="MIGDGVVGTHAARIALGLGAQVTILDISAKRLSVLEEVFGSQIQ
                     TLMSNSFNIEASVRDADVVIGAILIPGAKAPELVTDEMVKQMRPGSVSLTLLLTKVAL
                     SKQLTV"
     gene            complement(30456..30881)
                     /locus_tag="VYO70_000313"
     CDS             complement(30456..30881)
                     /locus_tag="VYO70_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEV1712605.1"
                     /translation="MLIGIPKEIKNNENRVALTPAGVHSLVSRGHRVLIETNAGLGSG
                     FTDADYQKQGAEIVATAGEAWAAELVVKVKEPLNSEYGYLRDDLLLFTYLHMAAAPEL
                     ADAMLAAKTTGIAYETVRDNQGQLPLLVPMSEVAGRMAV"
     gene            31058..31627
                     /locus_tag="VYO70_000314"
     CDS             31058..31627
                     /locus_tag="VYO70_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1712606.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKDNPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            31695..32345
                     /locus_tag="VYO70_000315"
     CDS             31695..32345
                     /locus_tag="VYO70_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEV1712607.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEEEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGGKTIEKLKDYVTVD"
     gene            32329..34569
                     /locus_tag="VYO70_000316"
     CDS             32329..34569
                     /locus_tag="VYO70_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1712608.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GYLNTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLIGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     LEVTKAFHVGEILVSKGSLKQKQFVVELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            34748..34936
                     /locus_tag="VYO70_000317"
     CDS             34748..34936
                     /locus_tag="VYO70_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712609.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHGHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            34969..35556
                     /locus_tag="VYO70_000318"
     CDS             34969..35556
                     /locus_tag="VYO70_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712610.1"
                     /translation="MLQLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            35560..36744
                     /locus_tag="VYO70_000319"
     CDS             35560..36744
                     /locus_tag="VYO70_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712611.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVNLNWSMGMVLLPCLIIFLLVGILFIESWGSSRKKEVFN
                     RS"
     misc_feature    36905..37036
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            37052..37582
                     /gene="infC"
                     /locus_tag="VYO70_000320"
     CDS             37052..37582
                     /gene="infC"
                     /locus_tag="VYO70_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1712612.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            37615..37815
                     /gene="rpmI"
                     /locus_tag="VYO70_000321"
     CDS             37615..37815
                     /gene="rpmI"
                     /locus_tag="VYO70_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1712613.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            37867..38226
                     /gene="rplT"
                     /locus_tag="VYO70_000322"
     CDS             37867..38226
                     /gene="rplT"
                     /locus_tag="VYO70_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1712614.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            38284..38664
                     /locus_tag="VYO70_000323"
     CDS             38284..38664
                     /locus_tag="VYO70_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1712615.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            38914..39714
                     /locus_tag="VYO70_000324"
     CDS             38914..39714
                     /locus_tag="VYO70_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1712616.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            39725..40663
                     /locus_tag="VYO70_000325"
     CDS             39725..40663
                     /locus_tag="VYO70_000325"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1712617.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            40705..42738
                     /locus_tag="VYO70_000326"
     CDS             40705..42738
                     /locus_tag="VYO70_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEV1712618.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKMAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNKNAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(42799..44454)
                     /gene="pavA"
                     /locus_tag="VYO70_000327"
     CDS             complement(42799..44454)
                     /gene="pavA"
                     /locus_tag="VYO70_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1712619.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTESLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            44559..45056
                     /gene="ybeY"
                     /locus_tag="VYO70_000328"
     CDS             44559..45056
                     /gene="ybeY"
                     /locus_tag="VYO70_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1712620.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            45037..45432
                     /locus_tag="VYO70_000329"
     CDS             45037..45432
                     /locus_tag="VYO70_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1712621.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENMVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAVLTGALIFLPRIWDLLF"
     gene            45449..46348
                     /gene="era"
                     /locus_tag="VYO70_000330"
     CDS             45449..46348
                     /gene="era"
                     /locus_tag="VYO70_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1712622.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEKEY"
     gene            46397..47221
                     /gene="mutM"
                     /locus_tag="VYO70_000331"
     CDS             46397..47221
                     /gene="mutM"
                     /locus_tag="VYO70_000331"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1712623.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            47221..47826
                     /locus_tag="VYO70_000332"
     CDS             47221..47826
                     /locus_tag="VYO70_000332"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1712624.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFEQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            47813..49012
                     /locus_tag="VYO70_000333"
     CDS             47813..49012
                     /locus_tag="VYO70_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEV1712625.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            49012..49161
                     /gene="rpmG"
                     /locus_tag="VYO70_000334"
     CDS             49012..49161
                     /gene="rpmG"
                     /locus_tag="VYO70_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1712626.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            49201..49434
                     /gene="secG"
                     /locus_tag="VYO70_000335"
     CDS             49201..49434
                     /gene="secG"
                     /locus_tag="VYO70_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1712627.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            49536..51890
                     /gene="rnr"
                     /locus_tag="VYO70_000336"
     CDS             49536..51890
                     /gene="rnr"
                     /locus_tag="VYO70_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1712628.1"
                     /translation="MKDRIKEYLQDKGKMTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVLSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGCSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKSFYKEVAKKGAKHGKGRGKGRRTK"
     gene            51853..52320
                     /gene="smpB"
                     /locus_tag="VYO70_000337"
     CDS             51853..52320
                     /gene="smpB"
                     /locus_tag="VYO70_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1712629.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            52336..53196
                     /gene="tehB"
                     /locus_tag="VYO70_000338"
     CDS             52336..53196
                     /gene="tehB"
                     /locus_tag="VYO70_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEV1712630.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            53269..54222
                     /gene="coiA"
                     /locus_tag="VYO70_000339"
     CDS             53269..54222
                     /gene="coiA"
                     /locus_tag="VYO70_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1712631.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            54241..56043
                     /gene="pepF"
                     /locus_tag="VYO70_000340"
     CDS             54241..56043
                     /gene="pepF"
                     /locus_tag="VYO70_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1712632.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENRGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTIFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNSEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            56045..56758
                     /locus_tag="VYO70_000341"
     CDS             56045..56758
                     /locus_tag="VYO70_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1712633.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFIMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            56825..57766
                     /gene="prsA"
                     /locus_tag="VYO70_000342"
     CDS             56825..57766
                     /gene="prsA"
                     /locus_tag="VYO70_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1712634.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(57885..58226)
                     /locus_tag="VYO70_000343"
                     /pseudo
     CDS             complement(57885..58226)
                     /locus_tag="VYO70_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        17544 a        10675 c        13188 g        16842 t
ORIGIN      
        1 aaaatacttt acaagttctt aaaaacatga tatagtaata aagcttagaa aatgagatga
       61 tgttttctag caaatataaa cccgagtaaa aaatgcctac ggacaggcag ggttgaatgc
      121 cgaagcgtgg ttgaaaagcc acattattga tagggttaaa agcctacttt tataagttga
      181 tgttaggaca cttgtcctaa ttcataaatt tttagtgtgg tgaaagcaca cgtcatcttg
      241 tgaaacgatc aataaagtac gtaatatttg ctactagaga gttaggaaac atcgggaaca
      301 gacatactca acagaaacca aaataaacac gtcagaagat tgcagagcag gtgaaaacct
      361 gctctttttt catgagtcaa cctttagttc cttagttttc ataaggtcct aaaaatattg
      421 aaaggagtat gttttgaaag agttagatca aaaccaagcc ccaatttatg aggccttggt
      481 gaagttacgc aagaaaagga ttgttccctt tgatgttcca ggtcacaagc gtggacgggg
      541 aaatccagaa cttgtcgaac tcttaggaga aaaatgtgta ggcattgatg tcaattcgat
      601 gaaacccttg gataatttag gccatcctat ttcgattatt cgtgatgcag aggagctggc
      661 tgcagatgct tttggagcta gccatgcctt tctaatgatt ggtggaacaa cttcatcggt
      721 gcagactatg attctggcaa cctgcaaggc aggagataag attattctgc cacgaaatgt
      781 ccataaatct gctatcaatg cgttggttct atgtggtgct attcccatct atatcgagat
      841 gagtgtagat cctaagattg gtatcgcttt aggtcttgaa aatgaccgag tagcacaggc
      901 cataaaggac catccagatg ctaaggctat cctaatcaac aatcctactt actacggcat
      961 ctgttcagac ctaaaggggt tgacagaaat ggctcatgaa gctggcatga tggttttagt
     1021 agatgaagcc cacggagcgc atttgcattt cactgataaa cttccaattt ctgctatgga
     1081 tgcaggggct gatatggcag cagtttccat gcataagtct ggtgggagtt tgacccaaag
     1141 ctccatttta cttatcgggg agcagatgaa ttctgaatac gttcgtcaga taattaacct
     1201 gacccagtct acatctgcct cttacttgtt gatggctagt ttggatattt cacgtcgcaa
     1261 cttggccctt cgtggtaaag agtcgtttga gaaagtcatt gagctatctg agtatgcccg
     1321 ccgtgaaatc aatgctatcg gtggctacta tgcctactca aaagagttaa tagacggtgt
     1381 ttcggtttgc gattttgacg taactaagtt gtcagtttac actcagggta ttggcttaac
     1441 aggtatcgag gtttatgacc tcttgcgaga cgaatacgac attcagatcg agtttggtga
     1501 tatcggcaat atcttggcct atatttccat cggcgaccgc atccaagaca tcgagcgctt
     1561 ggttggtgct ctggctgata ttaagagact ctattcaaga gatggaaaag atttgatagc
     1621 aggagaatat attcagcccg agttagtgct gtctccgcaa gaagccttct attcagaaag
     1681 aaaaagttta actttggatg attctgttgg acaggtctgt ggagaatttg ttatgtgtta
     1741 ccctccaggt attcctatct tggctcctgg tgaacgcatt acacgagaaa ttgtcgacta
     1801 tatccaattc gccaaggaac gtggttgctc cctccaaggg acggaagatc cagaggtcaa
     1861 tcatatcaac gtcattaaga gaaagacaaa ctataagaaa agtcaatagt tttatctaaa
     1921 ctatttctta tttcaatttg atgatttggc gatgatttta gagcacggca aaaagccctt
     1981 gaattagaag cggtcaatcg cttaatttct atcagcttat caaatcctgc ctcaatcctt
     2041 ttctgaggat tagggtagcg tgtcaagagt tggtaggtat attctgaatg ctttccaacg
     2101 attttatcca actcaggaaa gatgatatca agacaacgag tgtattgtac tttccaatca
     2161 gactgttttt cttgagacga tgaatatgtc tagccagtat ttttaggtct acttgccgat
     2221 tatcgtgttg aaattgttca cgattggggt cagaaagaag tttaagagcg atgccatgag
     2281 cgtctttctt atccgtttta gtcttgcgaa gtgataatga tttggcaaat tccttgatga
     2341 gcaaaggatt gtaggtgtaa actttatatc cttgttcatg caggaagttc agtagattaa
     2401 aggcataatg tccagtatct tcaagagcga tgagacagtc ttggttgagc tgtcgaagag
     2461 acagatctaa gagttcaaaa ccagctttat tatttgaaaa agtgagtggt ttaagaacag
     2521 ttttcctgaa acattcaagg ctgtaacatc gtgtttattt ttagcgacat caatgcccac
     2581 ataaagcatg ggagtacctc cagatatagt atttcaagtc tacttagtta tctacgaact
     2641 ttttgccttg ttaccttaga cgagataaaa cgtctatgcg ttatcaaact cattaccaat
     2701 tgaaacaaaa agctgtggtt agaccctttc ggaaatcgtc aagcgattgg aggaaatgaa
     2761 ctaatccaca gtggcttatt ccaagtatac cacttgggct ttggcagtag ctaactgcgc
     2821 taaatataat ataaggagaa atagatggat ttatggtttt ctgaagttca tactccagat
     2881 gtcaaattgt ctctgagaac agccaagcaa ctttacgctg gaaaaagtga atggcaggat
     2941 atcgaagtct tggatacgcc agcttttggg aaaatactga ttttaaatgg ccatgtcttg
     3001 ttctcagatg cggatgattt cgtctacaat gaaatgaccg ttcacgttcc catggctgtc
     3061 cacccaaatc caaagaaagt attggttatt gggggtggtg acggcggtgt tgcccaagta
     3121 ttaacactct atcctgaact ggaacaaatc gatatcgtgg aaccggatga gatgttggtc
     3181 gaggtctgtc gtgagtattt cccagacttt gctgcagggc tagatgatcc tcgtgttacc
     3241 atttactacc aaaatgggct acgctttttg cgaaactgcg aagatgatta cgatattatc
     3301 atcaacgatg cgacagatcc atttggccat acggaaggac tctttaccaa ggaattctac
     3361 ggcaatagtt atcgagctct gaaggaagac ggcatcatga tttaccagca tgggagtccc
     3421 ttctttgacg aggatgagtc ggcctgccga agcatgcacc gcaaggtcaa tcaagccttt
     3481 ccaatcagtc gggtttatca ggcccatatt ccaactagcc cagctggcta ttggttgttt
     3541 ggatttgcat cgaaaaaata ccaccctgtc aaagattttg acaaggaagg ttggaaaaaa
     3601 cgccagcttt tcacagaata ctacactgca aacttacacg tgggagcctt tatgttgccc
     3661 aagtatgttg aggacatttt agaagaagag gaaggaaaaa aatgagtcgt ttactagtta
     3721 ttggttgtgg gggcgttgcc caagttgcta tttcaaaaat ttgtcaagat agcgaaacat
     3781 ttacagagat tatgattgct agccgtacca agtcaaaatg cgatgacttg aaagcgaagc
     3841 tagaaggcaa aacaagtact aaaattgaaa ctgcagcact tgatgctgac aaggtagaag
     3901 aggtcattac cttgattgaa agctacaaac cagaagctgt tttgaatgta gctctgcctt
     3961 atcaagattt aaccattatg gatgcttgtt tggcaacagg tgttcactat atcgatacag
     4021 ccaactacga agcagaagac acagaagacc ctgagtggcg tgctatctac gaaaaacgtt
     4081 gtaaggaact tggttttaca gcctactttg actactcatg gcagtgggct tatcaagaga
     4141 aattcaaaga agcaggcttg actgctcttc ttggttctgg ttttgaccca ggtgtaacta
     4201 gtgtcttttc agcttatgcc ctcaaacact attttgatga aatccattat atcgacattt
     4261 tagactgtaa tggcggtgac cacggttatc catttgcaac caactttaat ccagaaatta
     4321 atctccgtga ggtttctgcg ccaggttctt actgggaaga tgggaaatgg gtcgaagtcg
     4381 aagctatgtc tatcaagcgt gagtatgatt tccctcaagt tggacaaaaa gatatgtatc
     4441 tccttcacca tgaagaaatc gaatcattgg ccaagaacat tccaggtgtc aaacgcattc
     4501 gtttctttat gacttttggt caatcttact tgacgcacat gaaatgtctt gaaaatgttg
     4561 gactccttcg tacggatacc attaacttta acggccaaga aattgttcca attcaatttt
     4621 tgaaagcctt gcttccagat cctgctagcc ttggcccacg tactgtaggt aaaaccaata
     4681 ttggatgtat ctttacaggt gtcaaagacg gtgtcaaaaa gactatctat atctacaatg
     4741 tctgcgacca tcaagagtgc tatgcagagg ttggttcgca agctatttct tatacgacag
     4801 gagttccagc catgattggg acaaaattag tcatgaacgg aacttggaaa caagctggag
     4861 tgtataacct tgaggagtta gatccagatc cattcatgga agctttgaat gagtatggtt
     4921 tgccatgggt tgtggttgaa aatccacaaa tggtggacta atgaagttag aacaagtacc
     4981 aacaccagcc tatgttattg acttggccaa gttagaagct aattgccgca ttctacaata
     5041 tgtacaagaa gaggccggtt gcaaggtctt gcttgcccag aaggcatatt ccctctacaa
     5101 aacttatccc ttgattagcc agtatctatc aggtacgaca gctagtggac tctatgaggc
     5161 caaattggca agggaagaat ttcctggtga agtccatgtt tttgcaccag ctttcaagga
     5221 tgaggacatg gaggaattgc ttgggataac agaccatatc gtctttaact cagagagaca
     5281 gttgcgtaag catggttcgc gttgtcgaga ggctggtgtc agtgttggtt tgcgcctcaa
     5341 ccctcagtgt tcaactcaag gagatcacgc gctctatgac ccttgtgcac caggttctcg
     5401 ctttggagtt actatagaca agattccgag tgatttgcta gatttggttg acggacttca
     5461 ttttcatacc ctttgcgagc agggagcaga tgatttacaa acaactttga aagaagtaga
     5521 agaacagttt ggtccctact tacatgaggt aaaatggctc aatatgggtg gtggtcatca
     5581 tattacaaga gaaggttacg atgtggattt gctgatttca gaaatcaagc gtatccgaaa
     5641 aacttacaat cttgaaatct atatcgagcc tggtgaagcc attgcgctta atgcgggtta
     5701 tttagcaact gaggtattag atattgtaga aaacggtatg gaaatcttgg ttttagacgc
     5761 ctctgcgacc tgccatatgc ctgatgtact tgagatgccc tatcgtccac ctttgagaaa
     5821 tggctttgag gcacaggaaa aagcccatac ctacagactt tcttctaata cctgtctgac
     5881 gggcgatgtg attggtgatt atagttttga aaatccagtc caaatcggag acagacttta
     5941 ttttcaagac atggccattt attcttttgt caaaaataat acctttaatg gtattggatt
     6001 gccaagtctc tatctcatgg acgaacaggg agactgtagc ttactcaaag cttttggcta
     6061 tcaagacttt aaagggagat tatcatgatg gacagtccaa aaaaattagg ctatcacatg
     6121 ccagcagagt acgaacccca tcatggtacc ctcatgatat ggccgactcg accaggatca
     6181 tggccttttc aaggaaaggc tgctaaaaga gcatttactc agatcatcga gaccatagca
     6241 gaaggggaaa gagtctatct tttggtggag caggcctatc tatctgaagc ccaatcctat
     6301 cttggagaca aggttgttta tttagacatt cccaccaatg atgcctgggc gcgtgatact
     6361 ggcccaacca ttctcgtcaa tgataaaggt aagaaattag ccgtggattg ggccttcaat
     6421 gcttggggag gcacctatga tggtctttat caagattatg aagaggatga ccaagtagcc
     6481 agtcgttttg ctgaggcctt ggaaaggcct gtctatgatg ctaaaccttt tgtactggaa
     6541 ggaggcgcaa tccatagcga tggtcaagga actattctcg taactgaaag ttgcttgctt
     6601 agtcctggtc gcaatcctaa cctgactaaa gaggagattg aaaacacatt attagaaagt
     6661 cttggtgctg aaaaagttat ttggcttcct tatggtattt atcaggatga aaccaatgaa
     6721 cacgtcgata atgttgctgc ctttgttggt cctgctgagc ttgttttggc ttggacagat
     6781 gacgaaaatg atccccagta tgccatgtca aaagcagatc tcgaactctt agaacaggaa
     6841 acagatgcaa aaggttgtca cttcaccatt cataaattgc ctatccctgc agttcgacaa
     6901 gttgtgacag aagaagattt gccaggctac atctatgaag aaggagaaga aaagcgatac
     6961 gcaggtgaac gactagcagc ttcctacgta aacttttata tcgccaacaa ggctgtcttg
     7021 gttccacagt ttgaggatgt aaacgaccaa gtggccttag atatcctcag caagtgtttc
     7081 ccagaccgta aagttgtcgg aataccagcc agagatattc tcttaggtgg tggcaatatc
     7141 cactgtatca cccaacaaat tccagaatag gagaaaaaga tgagaaatgt aagagttgca
     7201 accattcaga tgcaatgcgc taaggatgtg gcaacaaata tccaaaccgc agagcgttta
     7261 gtacgtcagg ctgctgagca aggagcccaa attattctct tgcccgagtt gtttgaacat
     7321 ccctatttct gtcaggaacg tcagtatgac tactaccagt atgcccaatc tgtagcggaa
     7381 aatactgcca ttcagcattt taaggtgatt gctaaggaac tacaagttgt tttaccaatc
     7441 agtttctatg aaaaagatgg taatgtcttg tataactcta ttgccgtcat tgatgcagat
     7501 ggggaagtgc tgggcgttta tcgaaagacc catataccag atgaccatta ttatcaagaa
     7561 aaattctatt tcacgcctgg taacactggt ttcaaggtct ggaatactcg ctatgctaag
     7621 attggtatcg gtatctgttg ggatcaatgg ttccctgaaa cagcgcgctg tcttgcattg
     7681 aatggtgctg aattgctctt ttatcctaca gctatcggtt cagagccaat tttggataca
     7741 gatagttgtg gtcactggca acgtactatg caagggcacg cagcagcgaa tattgttcca
     7801 gtcatcgcag ccaatcgtta tggtttagag gaggttactc ctagtgagga aaatggcgga
     7861 cagagttcca gtcttgactt ctacggttcc tcctttatga cggatgaaac aggagctatt
     7921 ctagaacgag ctgaaagaca agaagaagct gttctgttag ctacttatga cttagacaag
     7981 ggagcaagtg aacgcctaaa ctggggcttg tttcgagata gaagaccaga aatgtataga
     8041 caaattacag attagtgtgg gagaaatgag agattcattc tgctagacta acttcttatt
     8101 agcaactata agatactatg gcatctagta aatcgatttt tatgattcgc tattcttgtc
     8161 tattgattag tccgtatttt aaaatattag caaaaaagca aatagcagta acttctgtct
     8221 atttgctttt cttttttata gaatatattt ctcaatagca cgcgcaacgc cgtcttcttc
     8281 gttgcttgat gtaacggcat ccgcaagaga tttgacataa tcgctggcat ttcccattgc
     8341 aatcccaagc cctgcaaact ggagcatttc gatatcgtta ttagcatcgc ccatggccat
     8401 aatctctgag gaatcaatct tcaaaatctc agctagtcgt gaaagagcag tagcctttgt
     8461 cgttccaagc ggcattgctt cataaatgac aggctgcgaa cgaactccac tgaatcgttg
     8521 gcaaagctct tcagcaaaac gctgctcaaa atcgtctgtt tgttcttttg ttcctaaaaa
     8581 cataccttgg aacatccgga actttccact agtcgcttct tcaagagaaa tttcagtcag
     8641 gtctgaaaat actagtttag catcattttc aataacttga ttgggtttgc caccgagaac
     8701 aaaataatgt gactcgtcaa aaagtgtcaa ctgaacatca ctcttttcag caaggtcata
     8761 gaggtattcg atgtcagctg gactcagttc ttgccagtca accaagcccc agtcgctagt
     8821 ctggtgagtt gaacaaccgt tgttaacaat aatatattcg ttctggaggt caagctccag
     8881 ttttttgtag taggggagga caccgaaaag ggggcgaccc gtacagagaa ccagtttgac
     8941 acctttttca atggctttgt gaatagcagt aatgtgagct tgtgggattt ccttggcttc
     9001 attgaggagg gtgccgtcca tatccaaggc tagtagttta atcataggtc ttccttcttt
     9061 atcttttgct attattatag catattttgg agaagaaatt gatagaaagc ttgagactaa
     9121 ttgattttat agtttaagat gttttgatga caattcatga tttgaagagg atatttcgca
     9181 aagatatgct atactatgtt tgtcaatgtt gcaactagac aaattaaaaa accaacttaa
     9241 tataatagtt tttttgtaag taggtatgag tagcagatta ctcaactaat ctgaagaata
     9301 atggaggaaa tatatcatga ttttaatgac aaaaaatata aatctaacaa atgaagaatt
     9361 agagctgata caaggtggag cagatccata tggtaaaaat cctaatggta ggtacgattg
     9421 ggaaatagaa ccagtattaa ctctgctggt tcatggattt tgtcccagag gaaccgatga
     9481 tttaggatat attggaggag gtaatcatct ttgcaaagga agtgctgcga gattttaagt
     9541 aaaatttatt aggaatatga agaaacaagg ggagaaaaca gaggatttaa tatgaaaaaa
     9601 cgagctattc aaattttact agtattgtcc ttaatttttt acaaatcaac ttggttttgg
     9661 aggcttttca attatctcgc aaagccctat ctaccagcaa gtcgtgaatt ttttcagatt
     9721 cttcttttga tggagagtgg agttcttttc ttagcggtca tctatctact ggtttttgca
     9781 ggaaagaaaa tttttcattt caagtggcag ctgaggtact tcatctacct tttactgggc
     9841 tacatcattt catatatgtc tgacttcctc ttttcgtatt tcatatcccc gccttcaaat
     9901 caaatttctt tgaatgaaac gatagaaatg atgggacgac aggagttccc ttatttcttg
     9961 ctcatcgttt gcttcatcgc ccctattgct gaggaattga tttatcgcgg tgtgattatg
    10021 acaacctttt tcaaaaactc gccttggtac ggagatgttt tgctttctgc tattattttc
    10081 ggttatattc atatcaattt tgctttaact cctcttgctt ttttcattta tgctagtgga
    10141 ggtcttattt tagctctatt gtatcgcatg actaaaaatc tctactatcc aatactagtt
    10201 catattctca ttaatatcac tgccttctgg gatgtgtggt tgctcctatt ttcaggaagt
    10261 tagcttacta aaataatgtc ggaactttcc ggcattttct tttttcacaa atagtcaacg
    10321 tttttctttt cgatattgta gtggtgtgta gccagttatt tttttgaatt gattttgaaa
    10381 ataaggttgg cttgagaaag gcagatagtg aagatagtta agaagaatag gatgttcttt
    10441 tttccttttt ggaaaacttc taaaatatgg tataatgaaa agataaagaa gttaggggta
    10501 gaagatgaac attcaacaat tacgctatgt tgtggctatt gccaatagtg gtacttttcg
    10561 tgaagctgct gaaaagatgt atgttagtca gccgagtctg tctatttctg ttcgggattt
    10621 ggaaaaagag ttgggtttta agattttccg tcggaccagc tcagggactt tcttgacccg
    10681 tcgtgggatg gaattttatg aaaaagcgca agaattggtt aaaggatttg atatttttca
    10741 aaatcagtat gccaatcctg aagaagaaaa agatgaattt tccgttgcta gccagcacta
    10801 tgacttctta ccaccaacta ttacggcctt ttcagagcgc tatcctgact ataagaactt
    10861 ccgtattttt gaatcaacta ctgttcaaat attagatgaa gtggcgcaag ggcatagtga
    10921 gattgggatt atctacctca acaatcaaaa taaaaagggg attatgcaac gggttgaaaa
    10981 attaggtctg gaggtcatcg aattgattcc tttccatacc catatttatc tccgtgaggg
    11041 tcatccttta gcccagaaag atgaattagt catggaggat ttagcggatt taccaacggt
    11101 tcgtttcact caagagaaag acgagtacct ttattattca gagaactttg tcgataccag
    11161 cgctagctca cagatgttta atgtgacaga ccgtgccacc ttgaatggta ttttggagcg
    11221 gacggacgcc tatgcgacag gttctggatt tttagatagt gacagtgtta atggcattac
    11281 agttattcgt ctcaaggata acctagataa ccgcatggtc tatgttaaac gtgaagaagt
    11341 ggagcttagt caagctggga ctctcttcgt agaagtcatg caagaatatt ttgatcaaaa
    11401 gaggaaatca tgaaaaaaag agcaatagtg gcagtcattg tactgctttt aattgggctg
    11461 gatcagttgg tcaaatccta tatcgtccag cagattccac tgggtgaagt gcgctcctgg
    11521 atccccaatt tcgttagctt gacctacctg caaaatcgag gtgcagcctt ttctatctta
    11581 caagatcagc agttgttatt cgctgtcatt actctggttg tcgtgatagg tgccatttgg
    11641 tatttacata aacacatgga ggactcattc tggatggtct tgggtttgac tctaataatc
    11701 gcgggtggtc ttggaaactt tattgacagg gtcagtcagg gctttgttgt ggatatgttc
    11761 caccttgact ttatcaactt tgcaattttc aatgtggcag atagctatct gacggttgga
    11821 gtgattattt tattgattgc aatgctaaaa gaggaaataa atggaaatta aaattgaaac
    11881 tggtggtctg cgtttggata aggctttgtc agatttgtca gaattatcac gtagtctcgc
    11941 gaatgaacaa attaaatcag gccaggtctt ggtcaatggt caagtcaaga aagctaaata
    12001 cacagtccaa gagggtgatg tcgtcactta ccatgtgcca gaaccagagg tattagagta
    12061 tgtggctgag gatcttccgc tagaaatagt ctaccaagat gaggatgtgg ctgtcgttaa
    12121 caaacctcag ggaatggttg tgcacccgag tgctggtcat accagtggaa ccctagtaaa
    12181 tgccctcatg tatcatatta aggacttgtc gggtatcaat ggggttctgc gtccagggat
    12241 tgttcaccgt attgataagg atacgtcagg tcttctcatg attgctaaaa acgatgatgc
    12301 gcatctagca cttgcccaag aactcaagga taaaaagtct ctccgcaaat attgggcgat
    12361 tgttcatgga aatctgccta atgatcgtgg tgtaattgaa gcgccgattg gccggagtga
    12421 aaaagaccgt aagaaacagg ctgtaactgc taaagggaag cctgcagtga cgcgttttca
    12481 cgtcttggaa cgctttggcg attatagctt agtagagttg caactggaga cagggcgtac
    12541 tcatcaaatc cgtgtccaca tggcttatat cggccatcca gtcgctggtg atgaggtcta
    12601 tggtcctcgc aagactttga aaggacatgg acaatttctt catgccaaga ctttaggttt
    12661 tactcatccg agaacaggta agaccttgga atttaaagca gatatcccag agatttttaa
    12721 ggaaaccttg gagagattga gaaagtaaga atgaaaaaga aattaactag tttagcactt
    12781 gtaggcgctt ttttaggttt gtcatggtat gggaatgttc aggctcaaga aagttcagga
    12841 aataaaatcc actttatcaa tgttcaagaa ggtggcagtg atgcgattat tcttgaaagc
    12901 aatggacatt ttgccatggt ggatacagga gaagattatg atttcccaga tggaagtgat
    12961 tctcgctatc catggagaca gggtattaat accacttata agcatgttct gacagaccgt
    13021 gtctttcgtc gtttgaagga attgggtgtc caaaaacttg attttatttt ggtgacccat
    13081 acccacagtg atcatattgg aaatgttgat gaattactgt ctacctatcc agttgaccga
    13141 gtctatctta agaaatatag tgatagtcgt attactaatt ctgaacgtct atgggataat
    13201 ctgtatggct atgataaggt tttacagact gctgcagaaa aaggtgtttc agttattcaa
    13261 aatatcacac aaggggatgc tcattttcag tttggggaca tggatattca gctctataat
    13321 tatgaaaatg aaactgattc atcgggtgaa ttaaagaaaa tttgggatga caattccaat
    13381 tccttgatta gcgtggtgaa agtcaatggc aagaaaattt accttggggg cgatttagat
    13441 aatgttcatg gagcagaaga caagtatggt cctctcattg gaaaagttga tttgatgaag
    13501 tttaatcatc accatgatac caacaaatca aataccaagg atttcattaa aaatttgagt
    13561 ccgagtttga ttgttcaaac ttcggatagt ctaccttgga aaaatggtgt tgatagtgag
    13621 tatgttaatt ggctcaaaga acgaggaatt gagagaatca acgcagccag caaagactat
    13681 gatgcaacag tttttgatat tcgacaagac ggtttagtaa acatttcaac ttcctacaag
    13741 ccgattccaa gttttcaagc tggttggcat aagagttcat atggaaactg gtggtatcaa
    13801 gcgcctgatt ctacaggaga atatgctgtt ggttggaatg aaattaaggg tgaatggtat
    13861 tactttaacc aaacgggtat cttattacag aatcaatgga aaaaatggaa caatcgttgg
    13921 ttctatttga cagactctgg tgttgctgct aaaaattgga agaaaatcgc tggaatctgg
    13981 tattatttta acaaagaaaa ccagatggaa attggttgga ttcaagataa agagcagtgg
    14041 tattatttgg atgttgatgg ctctatgaag acaggttggc ttcaatataa ggggcaatgg
    14101 tattactttg ctccatcagg ggaaatgaaa acgggctggg taaaagataa agaagcctgg
    14161 tactatatgg attctactgg tatcatgaag acaggtgaga tagaagttgc tggacatcat
    14221 tactatctag aagaatcagg agctatgaag caaggctggc ttaaaaaggc aaatgattgg
    14281 tatttctaca agacagatgg ttcacgagct ataggttgga ttaaagacaa ggataaatgg
    14341 tacttcttga aagaaaatgg tcaattactt gtgaatggta agacaccaga aggctatact
    14401 gtggattcaa gtggtgcctg gttagtggat gttccgatcg agaaatctgc tacaattaaa
    14461 actacaagtc attcagaaat aaaagaatcc aaagaagtag tgaaaaagga tcttgaaaat
    14521 aaagaaacga gtcaacatga aagtgttaca aattcttcaa ctagtcaaga tttgacatcc
    14581 tcaacttcac aaagctctga aacgagtgta aacaaatcgg aatcagaaca gtagtagaaa
    14641 agaaggtttt agggccttct ttttcctatc aactcttttc tatttcctgt tattcatgtt
    14701 ataatggata aatatgaata atcggagtga gactatgaaa tacaaacgga ttgtctttaa
    14761 ggtgggtact tcttctctga caaatgagga tggaagttta tcacgtagta aggtaaagga
    14821 tattacccag cagttggcta tgctgcacga ggctggtcat gagttgattt tggtgtcttc
    14881 aggtgccatt gcggctggtt ttggagcctt aggatttaaa aagcgtccga ctaaaattgc
    14941 tgataaacag gcttcagcag cggtagggca ggggcttttg ttggaagaat atacaaccaa
    15001 tcttctcttg cgtcaaatcg tttctgcaca aatcttgctg acccaagatg actttgtgga
    15061 taagcgtcgt tataaaaatg cccatcaggc tttgtcggtt ttgctcaacc gtggggcaat
    15121 tcctatcatc aatgagaatg atagtgtcgt tattgatgag ctcaaggttg gggacaatga
    15181 cactctaagt gctcaagtag cggcgatggt ccaagcagac cttttagttt tcttgacaga
    15241 tgtggacggt ctctatactg gaaatcctaa ttcagatcca agagccaaac gcttggagag
    15301 aatcgagacc atcaatcgtg agattattga tatggctggt ggagctggtt cgtcaaacgg
    15361 aactgggggt atgttaacca aaatcaaggc tgcaactatc gcgacggaat caggagttcc
    15421 tgtttatatc tgctcatcct tgaaatcaga ttccatgatt gaggcggcag aggagaccga
    15481 ggatggttct tactttgttg ctcaagagaa ggggcttcgt acccagaaac aatggcttgc
    15541 cttctatgct cagagtcaag gttctatttg ggttgataaa ggggctgcgg aagctctctc
    15601 tcaacatgga aagagtcttc tcttatctgg tatcgttgaa gcagaaggag tcttttctta
    15661 cggtgatatc gtgacagtat ttgataagga aagtggaaaa tcacttggaa aaggacgcgt
    15721 gcaatttgga gcatctgctt tggaggatat gttgcgttct caaaaagcca agggtgtctt
    15781 gatttaccgt gacgactgga tttccattac tcctgaaatc caactacttt ttacagaatt
    15841 ttagaggtaa actatggtga gtagacaaga acaatttgaa caggtacagg ctgttaaaaa
    15901 atcgattaac acagctagtg aagaagtgaa aaaccaagcc ttgctagcca tggctgatca
    15961 cttagtggct gctactgagg aaattttagc ggctaatgcc ctcgatatgg cagcggctaa
    16021 ggggaaaatc tcagatgtga tgttggatcg tctttatttg gatgcagatc gtatagaagc
    16081 gatggcaaga ggaattcgtg aagtggttgc cttaccagat ccaatcggtg aagttttaga
    16141 aacaagtcag cttgaaaatg gtttggttat cacaaaaaaa cgtgtagcta tgggtgtcat
    16201 cggtattatc tatgaaagcc gtccaaatgt gacgtctgat gcggctgctt tgactcttaa
    16261 gagtggaaat gcggttgttc ttcgtagtgg taaggatgcc tatcaaacaa cccatgccat
    16321 tgtcacagcc ttgaagaagg gcttggagac gactactatt catccaaatg tgattcaact
    16381 ggtggaggat actagccgtg aaagtagtta tgctatgatg aaggccaagg gctatctaga
    16441 ccttctcatt cctcgtggag gagctggctt gatcaatgca gtggttgaga atgcgattgt
    16501 acctgttatc gagacaggga ctgggattgt ccatgtctat gtggataagg atgcagacga
    16561 agacaaggcg ctgtctatca tcaacaatgc taaaaccagt cgtccttctg tttgtaatgc
    16621 catggaggtt ctgctggttc atgaaaacaa ggcagcaagc atccttcctc gcttggatca
    16681 aatgctggtt gcagagcgta aggaagctgg actggaacca attcaattcc gcttggatag
    16741 caaagcaagc cagtttgttt caggtcaagc agctgagacc caagactttg acaccgagtt
    16801 tttagactat gtccttgctg ttaaggttgt gagcagttta gaagaagcgg ttgcgcacat
    16861 tgaatcccac agcacccatc attcggatgc tattgtgacg gaaaatgctg aagctgcagc
    16921 atactttaca gatcaagtgg actctgcagc ggtgtatgtt aatgcctcaa ctcgtttcac
    16981 agatggagga caatttggtc ttggttgtga aatggggatt tctactcaga aattgcacgc
    17041 gcgtggtccc atgggcttga aggaattgac tagctacaag tatgtggtca ctggtgatgg
    17101 acagataagg gagtaaggga tgaagattgg atttatcggt ttggggaata tgggtgctag
    17161 tttagccaag tctgtcttgc aggctagacc gtcagatgag attctccttg ccaatcgtag
    17221 tcaagtcaag gtggatgctt tcatcgctaa cttcggtggt cagacttcta acaatgaaga
    17281 aatattcgaa gaagcagatg tgatttttct aggtgttaag cctgctcagt tttctgaact
    17341 gctttctcaa taccagacca tccttgaaaa aagagaaagt cttcttttga tttcgatggc
    17401 agctggattg accttagaaa aactagcaag tcttatccca agccaacacc gaattattcg
    17461 tatgatgcct aatacccctg cttctatcgg gcaaggagtg attagttatg ccttgtctcc
    17521 taattgcagg gctgaggaca gtgagctctt ttgtcagctt ttagccaagg ctggtctctt
    17581 ggttgaatta ggagaaagct taatcaatgc agcgacaggt cttgcaggtt gtggaccagc
    17641 ctttgtctat ctctttattg aggccttggc agatgcaggt gttcagacgg gattaccacg
    17701 agaaacagcc ttgaaaatgg cagcccaaac tgtggtagga gctgggcaat tggtcctaga
    17761 aagccagcaa catcctggag tattgaaaga ccaagtctgt agcccaggcg gttcgactat
    17821 cgctggtgta gcaagcctag aagcgcatgc tttccgagga acagtcatgg atgcagttca
    17881 tcaagcctac aaacgaacac aagaactatg aaatgagaag acacaaaaag attgtcacaa
    17941 acccctattt ttttgataga atagaagtag taaaaaagaa atgagttaga catgtcaaaa
    18001 ggatttttag tctctcttga gggaccagag ggagcaggca agaccagtgt tttagaggct
    18061 ctgctaccaa ttttagagga aaaaggagta aaggtgctga cgacccgtga acctggcgga
    18121 gtcttgattg gggagaagat tcgggaagtg attttggatc caagtcatac tcagatggat
    18181 gctaaaacag agctacttct ctatattgcc agtcgcagac agcatttggt ggaaaaagtt
    18241 cttccagccc ttgaagctgg caagttggtc atcatggatc gttttatcga tagttctgtt
    18301 gcctatcagg gatttggtcg tggcttagat attgaagcca ttgactggct caatcagttt
    18361 gcgacagatg gcctcaaacc cgatttgaca ctctattttg acatcgaggt ggaagaaggg
    18421 ctggctcgta ttgctgctaa tagtgaccgc gaggttaatc gtttggattt ggaagggttg
    18481 gacttgcata aaaaagttcg tcaaggctac ctttctctcc tggaaaaaga aggaaatcgc
    18541 attgtcaaga ttgatgctag tctccctttg gagcaagttg tggaaactac caaggctgtc
    18601 ttgtttgacg gaatgggctt ggccaaatga aacaagatca actaaaggct tggcaaccag
    18661 ctcagtttga ccgttttgtc cgtatcttag aacaagacca gctcaatcac gcctatctct
    18721 tttcaggttt ctttggaagc ttggaaatgg cgcaattttt agctaagagc ctcttttgta
    18781 cggataaagt tggcgtctta ccatgtgaga aatgccgaag ttgcaagctg attgaacagg
    18841 aagagtttcc agatgtcacc ttgattaagc cagtcaatca ggtcatcaag acagaacgca
    18901 ttcgggaatt ggtgggacag ttttctcaag cagggattga aagccagcaa caggtcttta
    18961 tcatcgagca agcggataaa atgcatccca acgcagccaa ttctctgctc aaggtcatcg
    19021 aagaacccca gagtgaagtt tatattttct tcttgactag cgatgaggaa aagatgttac
    19081 cgacaatccg aagtcggact cagatcttcc actttaaaaa gcaagaagaa aaactcatct
    19141 tactcttaga acaaatggga cttgttaaga aaaaagcgac tcttttaact aagtttagtc
    19201 aatcgcgagc tgaagcagaa aagttggcta atcaggcaag tttttggacc ttggtcgatg
    19261 aaagtgaacg cctgctgact tggttagtag ctaagaaaaa agaaagttat ctacaggttg
    19321 ccaaattagc caacttggca gatgataagg aaaaacagga tcaggtttta cggattcttg
    19381 aagttctctg tgggcaggac ctcttgcagg taagagtaag agtgattcta caagatttac
    19441 tagaagctag aaaaatgtgg caagctaatg tcagctttca aaatgccatg gaatatctgg
    19501 tcttgaaaga aatataaact caaaaatgaa tgataaagaa aggaaagggc tgttttatgg
    19561 acaaaaaaga attatttgac gcgctggatg atttttccca acaattattg gtaaccttag
    19621 ccgatgtgga agccatcaag aaaaatctca agagcttggt agaagaaaat acagctcttc
    19681 gcttggaaaa tagtaagttg cgagaacgct tgggtgaggt ggaagcagac gctcccgtca
    19741 aggccaagca tgttcgcgaa agtgtccgtc gtatttaccg tgatggattt cacgtatgta
    19801 atgattttta tggacaacgt cgagagcagg acgaagaatg tatgttctgt gatgagttgt
    19861 tatacaggga gtaggcatgc aaattcaaaa aagttttaag gggcagtctc cctatggcaa
    19921 gctgtatcta gtggcaacgc cgattggcaa tctagatgat atgacttttc gtgctatcca
    19981 gaccttgaaa gaagtagatt ggattgctgc tgaggatacg cgcaatacag ggcttttgct
    20041 caagcatttt gacatttcca ccaagcagat cagttttcat gagcacaatg ccaaggaaaa
    20101 aattcctgat ttgattggtt tcttgaaagc agggcaaagt attgctcagg tctctgatgc
    20161 cggtttgcct agcatttcag accctggtca tgatttagtt aaggcagcta ttgaggaaga
    20221 aattgcagtt gttacagttc caggtgcctc tgcaggaatt tctgccttga ttgccagtgg
    20281 tttagcgccg cagccacata tcttttacgg ctttttaccg agaaaatcag gccagcagaa
    20341 gcaatttttt gactcgaaaa aagattatcc tgaaacacag attttttatg aatcacctca
    20401 tcgtgtagca gacacgttgg aaaatatgtt agaagtctac ggtgaccgct ccgttgtctt
    20461 ggtcagggaa ttgaccaaaa tctatgaaga ataccaaaga ggtacaattt ctgaattgct
    20521 ggaaagcatc tctgaaacgt ctctcaaggg tgaatgtctt ctgattgttg aaggtgccag
    20581 caaaggtgtg gaagaaaaag acgaggaaga cttgttctta gaaatccaag ctcgtatcca
    20641 gcaaggtatg aagaaaaatc aagctattaa ggaagtagct aagatttacc agtggaataa
    20701 gagtcaactc tacgctgcct accacgactg ggaagaaaaa caataaaggg agacaggatg
    20761 taataattct gtctgtttct gtttaactta attagtgatg ataatataaa gatgtatcac
    20821 ttggtataga agctttggta ttaagttttt tattaagccc atacggaata ccgatggttg
    20881 gagcagcagt tatagcgttc ttagaaggta taaatagaaa aataaggtca ttttaaatca
    20941 aaggattgat aaatcagaaa gaaggtgatt ttttgcgaac atacgaaaat aaagaagaac
    21001 taaaagctga gatagagaaa acatttgaga aatatatttt agaatttgat aatattccag
    21061 aaaatttaaa agataagaga gctgatgaag ttgacagaac tccagcagaa aaccttgctt
    21121 atcaggttgg ttggaccaac ttggttctta aatgggaaga agatgaaaga aaggggcttc
    21181 aagtaaaaac accatcggat aaatttaaat ggaatcaact tggtgagtta tatcagtggt
    21241 tcacagatac ctacgctcat ttatctctgc aagagttgaa agcaaaatta aatgaaaata
    21301 ttaattctat ctctgcaatg attgattcgt tgagtgagga agaattattt gaaccgcata
    21361 tgagaaagtg ggctgatgaa gcgactaaaa cagcgacttg ggaagtgtat aagtttattc
    21421 atgtaaatac ggttgcacct tttggaactt tcagaactaa aatcagaaaa tggaagaaga
    21481 tagtattata aattatattt ttaactttaa aaaatttcat aaaaatggtt accaaaggcg
    21541 atagaagaaa aactatcgtc tttttctttg caaattttta agaagggagg tgatcttgca
    21601 tggactttga atatttttat aacagagaag cggaaagatt taacttctta aaagtaccgg
    21661 agatattagt tgatagagaa gaatttcggg gcttatcagc agaagcaatt atcctttatt
    21721 ccatacttct taaacagaca ggaatgtcat ttaagaataa ctggatagac aaggaaggca
    21781 gagtatttat ctattttact gtcgaagaaa ttatgaaaag aagaaatatc tcaaagccaa
    21841 ctgccataaa aacattagat gagcttgatg taaaaaaagg aataggactg atcgaaagag
    21901 taaggcttgg acttggtaag ccgaatatca tttatgttaa agactttatg agtatatttc
    21961 aggtaaaaga aaatgactta cagaagtcaa aaaacttaac ttcagaagta aaagatttta
    22021 acctcagaag taaagaaaat gaacttcaag aggttaagaa ccttgactct aactatatag
    22081 agaataataa gagtaagtat agtaagagag aatatagttt tggtgaaaac ggacttggaa
    22141 catttcaaaa tgtgttttta gctgctgaag atatatcgga tttacaaatc ataatgaact
    22201 cacagcttga gaattacatt agacttcctg caaaactaga atcctagttc atgattgata
    22261 ataccagcac tcaaattcat tcgtaatccg aagcgtttac gatgatttcg ataggttgtt
    22321 gaaaacattt taaacgtttt tactttggca aagatgttct caaccttgct tctctcctta
    22381 gatagcgcat ggttataggc tttatcttca gctattagcg gcttgagttt gctggattta
    22441 cgtggagttt gtgcttgagg atatatcttc atgagccctt gataaccact gtcagccaag
    22501 attttaccag cttgtccgat atttctgcga ctcattttga acaacttcat atcatgacaa
    22561 tagttcacag tgatatccaa agaaacaatt ctcccttgac ttgtgacaat cgcctgagac
    22621 ttcatagcgt ggcatttctt tttaccagaa tcattcgcta attctttttt tagggcgatt
    22681 gatttttact ttcgtcgcat caatcattac cgtgtcctca gaactgagag gagttcttga
    22741 aatcgtaaaa ccactttgaa caagagttac ttcaacccat tggctccgac ggagtaagtt
    22801 gctttcgtgg ataccaaaat cagccgcaat ttcttcataa gttcgatatt ctcgcacata
    22861 gtgaagagtg gccataagaa ggtcttctag gcttaattta ggttttcgtc caccttttgc
    22921 gtgtttaagt tgataagctg tttttaatac agctaacatc tcttcaaaag tggtacgctg
    22981 aacaccaaca agacgcttaa atcgtgcatc agttagttgt ttacttgctt catcattcat
    23041 agaactacta taccatattt tgtttcgcag gaagtctatt ggaaagtaag aaatattgaa
    23101 gctgaggcta ttagaagaaa ttgtgagcgt ggtgctattt tttcaggtaa aaataaaata
    23161 tcacgaagat tcacagttta aaggagatca ctatgttgaa tgttatgctg ttttagataa
    23221 tacggttata gcaagagata gaataacagt ccctatcgat ccgttatgtg gaaaagattt
    23281 tatagagtag catataattg attcttaact ggaatactca ctatctcttt acatcaagaa
    23341 aatgactaaa cagggaagtt tgccttcttc cctttttttg ttatactagt agaagaaaaa
    23401 atagaaagat ttgtgggagt gaaaaagtcc tgtggacttt ttcagcctga gccaagaaac
    23461 tcgaaagctc gtaagtctga ttggcttttt caatgtgaat cttaacttca cactcccaaa
    23521 gaggtattag tgtcgtgtct caatcttata tcaatgttat cggtgctggt ttggcaggtt
    23581 ctgaagcagc ttaccaaatc gcagagcgtg gtattccagt taaactatat gaaatgcgtg
    23641 gtgtcaagtc tacaccccag cataaaacag acaattttgc tgagttggtt tgttccaatt
    23701 ctttgcgtgg ggatgctttg acaaatgcag ttggtcttct caaggaagaa atgcgtcgct
    23761 tgggttctgt tatcttggaa tctgctgagg ctacacgtgt tcctgcaggt ggtgcccttg
    23821 cagtggaccg tgatggtttc tctcaaatgg tgaccgaaaa agttgccaac caccccttga
    23881 ttgaagtggt tcgtgatgaa attacagaat tgccgacaga tgttattacg gttatcgcta
    23941 ctggtccttt gacaagtgat gccttagctg aaaagattca tgctcttaat gacggtgctg
    24001 gtttttattt ctacgatgcg gcagcgccta ttatcgatgt caacactatc gatatgagca
    24061 aggtctacct caaatcacgt tatgataagg gagaagcggc ctacctcaat gcccctatga
    24121 ccaagcaaga atttatggat ttccatgaag ctttggtcaa tgcagaagaa gcaccgctta
    24181 attcttttga aaaagaaaag tactttgaag gatgtatgcc tatcgaagtc atggccaaac
    24241 gtggcattaa aactatgctt tatggcccta tgaagccagt cggtcttgag tacccagacg
    24301 actatacagg acctcgtgat ggagaattta aaacacctta tgcggttgtg caacttcgtc
    24361 aggataatgc agctggtagc ctctacaata ttgttggttt ccagacccac ctcaaatggg
    24421 gagaacaaaa gcgtgtcttc caaatgattc cgggtcttga aaatgcggag tttgtccgtt
    24481 atggtgtgat gcatcgcaat tcttacatgg attcaccaaa tcttcttgag cagacttacc
    24541 gttctaagaa acaaccaaat ctcttctttg ctggtcagat gacgggggtg gaaggctatg
    24601 ttgagtcggc ggcttcgggc ttagttgcgg gaattaacgc agctcgtctc ttcaaggaag
    24661 aaagcgaggt tattttcccc gagacgacag cgattggaag cttagctcat tacattaccc
    24721 atgccgacag caaacatttc caaccaatga atgtcaattt tgggatcatc aaggagttgg
    24781 aaggcgagcg tatccgtgat aagaaggctc gttatgaaaa aattgcagag cgtgcccttg
    24841 ccgacttaga ggaatttttg actgtctaat ttttttgaaa gaattgctca tgatactata
    24901 aaaatcttag aaattgtgat aaaataggta ggatgaaaga aggagagtga aaatggcgaa
    24961 tcccaagtat aaacgtattt taatcaagtt atcaggtgaa gcccttgccg gtgaacgtgg
    25021 cgtagggatt gatatccaaa cagttcaaac aatcgcaaaa gagattcaag aagttcatag
    25081 cttaggtatc gaaattgccc ttgttattgg tggaggaaat ctctggcgtg gagaacctgc
    25141 agcagaagca ggtatggacc gtgttcaggc agattacact ggaatgcttg ggactgttat
    25201 gaatgctctt gtgatggcag attcattgca acaagttggg gttgatacgc gtgtacaaac
    25261 agctattgcc atgcaacaag tggcagagcc ttatgtccgt ggacgtgccc ttcgtcacct
    25321 tgaaaaaggc cgtatcgtta tctttggtgc tggaattggt tcaccatact tctcgacaga
    25381 tacaacagcg gctcttcgcg cagctgaaat cgaagcagat gccatcctca tggctaaaaa
    25441 tggcgtcgat ggtgtgtaca atgccgatcc taagaaggac aagacagccg ttaagtttga
    25501 agaattgacc caccgtgatg ttatcaacaa aggtcttcgt atcatggact caacagcctc
    25561 aaccctctca atggacaacg acattgactt ggttgtcttc aacatgaacc aaccaggaaa
    25621 catcaaacgt gtcgtatttg gtgaaaatat cggaacaaca gtttcaaata atatcgaaga
    25681 aaaggaataa gaaagaatat ggctaacgca attattgaaa aagctaaaga gagaatgacc
    25741 cagtctcacc aatcacttgc tcgtgaattt ggtggtatcc gtgctggtcg tgccaatgca
    25801 agcttgcttg accgtgtaca tgtagaatac tatggagtcg aaactcctct taaccaaatc
    25861 gcttcaatta cgattccaga agcgcgtgtt ttgttggtaa caccatttga caagtcttca
    25921 ttgaaagaca tcgaacgtgc cttgaacgct tctgatcttg gtatcacacc ggctaatgac
    25981 ggttctgtga ttcgcttggt tatcccagct cttacagaag aaactcgtcg tgaccttgct
    26041 aaagaagtga agaaggtcgg cgaaaatgct aaagtggctg tccgcaatat ccgtcgcgat
    26101 gctatggacg aagctaagaa acgagaaaaa gcaaaagaaa tcactgaaga cgaattgaag
    26161 actcttgaaa aagacattca aaaagtaaca gacgatgctg ttaaacacat cgacgacatg
    26221 actgctaaca aagagaaaga acttttggaa gtctaaaaat aaacagaaaa actcagttgg
    26281 cattgctggc tgagttttat tcgaaagaag gaaatatgaa tacaaatctt gcaagtttta
    26341 tcgttggact gatcatcgat gaaaacgacc gtttttactt tgtgcaaaag gatggtcaaa
    26401 cctatgctct tgctaaggaa gaaggccaac atacagtagg ggatacggtc aaaggttttg
    26461 catacacgga tatgaagcaa aaactccgcc tgacaacctt agaagtgact gccactcagg
    26521 accaatttgg ttggggacgt gtcacagagg ttcgtaagga cttgggtgtc tttgtggata
    26581 caggccttcc tgacaaggaa atcgttgtgt cactcgatat tctccctgag ctcaaggaac
    26641 tctggcctaa gaagggcgac caactctaca tccgtcttga agtggataag aaagaccgta
    26701 tctggggcct cttggcttat caagaagact tccaacgtct tgctcgtcct gcctacaaca
    26761 acatgcagaa ccaaaactgg ccagccattg tttaccgtct caagctgtca ggaacttttg
    26821 tttacctacc agaaaataat atgcttggtt ttattcatcc tagcgagcgt tacgcagagc
    26881 cacgtttggg gcaagtatta gatgcgcgcg ttattggttt ccgtgaagtg gaccgcactc
    26941 tgaacctctc cctcaaacca cgctcctttg aaatgttgga aaacgatgct cagatgattt
    27001 tgacttattt ggaaagtaat ggcggtttca tgaccttaaa tgataagtca tctccagacg
    27061 acatcaaggc aacctttggc atttctaaag gtcagttcaa gaaagcttta ggtggtctta
    27121 tgaaggctgg taaaatcaag caggaccagt ttgggacaga gttgatttag ggaggcttat
    27181 gagaaaatca ttttacactt ggctcatgac cgagcgcaat cctaaaagta acagtcccaa
    27241 agcaattttg gcagacctcg cttttgaaga ggcagctttt ccaaaacaca cagatgattt
    27301 tgatgaggtc agtcgctttt tggaggagca tgccagtttc tcttttaacc taggagactt
    27361 tgacgctatc tggcaagaat acttagaaca ctagcattta ttcactaggt ttgggctagt
    27421 aatttctcca tccctctgct ataataaaaa gaaataaaag gattagagag gttctttatt
    27481 tgaaggaaca ttcaatagac attcaactga gtcatccaga tgacctgttt catctttttg
    27541 gttccaatga acgccatctt cgtttgatgg aagaagagct tgatgttgtg attcatgctc
    27601 gtacggagat tgtccaggtt ttgggagaag agtctgcctg tgaggaagcc cgtcaagtta
    27661 ttcaggcttt gatggtcttg gtaaatcgtg ggatgaccgt tggtacgcca gatgtggtga
    27721 ctgcaattag catggtcaaa aacgatgaaa ttgataagtt tgtcgccctt tacgaagaag
    27781 aaattatcaa ggataatact gggaaaccga tccgcgttaa aacgttaggt caaaaacttt
    27841 atgtggacag tgtcaaacag catgatgtga cctttggaat tgggcctgca gggacgggga
    27901 agacctttct tgcagtgacc ttggcagtga ctgcccttaa acgtgggcaa gtcaagcgga
    27961 ttatcttgac acgtccagcg gtggaagcag gagagagtct aggttttctt ccgggtgatc
    28021 ttaaggagaa ggtagatcct taccttagac ctgtttatga tgccttgtat cagattctgg
    28081 ggaaagacca aacgactcgt ctcatggagc gtgaaattat cgaaattgcg ccccttgcct
    28141 atatgcgtgg ccggaccttg gatgatgcct ttgtcattct cgatgaggcg caaaacacga
    28201 ccatcatgca gatgaagatg ttcttgacgc gtttaggctt tcattctaag atgattgtca
    28261 atggagatat cagtcagatt gacctgccac gtaatgtcaa gtccggtttg attgatgctc
    28321 aagagaaact caagaacatc catcagattg actttgttca tttttcagcc aaggatgtgg
    28381 ttcgccatcc agttgtcgct cagattatcc gagcctatga atattctact gaagttgcac
    28441 acgactgatt ttgaggaagt tcgcctgcaa aagaatatac ttttgagaga ggaaaatcca
    28501 gttgtatagg ctaaaggttt tatccaaagg tctgagacaa cgattaggca cgatggaaag
    28561 aacttttatg tggctgatga cgatcagtgc atcttcctgt gtcataatca cagggcacaa
    28621 gaaagtagga atttgaaaag atgattgacc aactatctaa gtattacagt tgtaggatac
    28681 taactgaaaa ggatattcca agtattttat ctttatatga aagtaatcct ctgtattttc
    28741 agcattgtcc accagagcca aattttgcaa ctgtaaaaga ggacatgctt tgtctacctg
    28801 aaggtaaagc taaggctgat aagttttttg ttggattttg gaatggatct gaccttgtgg
    28861 ctgttatgga ttttgtctat gcatatcctg atgaggagac tgtttttatt ggtttgttta
    28921 tggttgatca agcctatcag agaaaaggga ttggtagtca tattgtgaca gaagcactag
    28981 cttattttgc taagaacttt cgaaaggcac gtttggctta tgttaaggga aatccgcaat
    29041 ctcagcattt ttgggaaaag cagggcttta aatcaattgg atgcgaggtt aagcaagaac
    29101 tctatacggt tgttatcgct gaacagagcc tagaagatta gaaatggcat caagtaagaa
    29161 ctatttggaa tttgttttgg aacaattatc aggattagat gatgtgactt accgttccat
    29221 gatgggggag tatattcttt acttccgcgg caagattatt ggcggcattt atgacgatcg
    29281 ctttttagtt aaacccgtgc aagcagtctt agataagatt gaccaatctt cttttgagtt
    29341 tccatacaaa ggtgccaaag aaatgatttg agtggaagaa cttgataata agatgtttct
    29401 ataagacctg attttagcta tgtataacca actgccaacg cccaaaccta aaaagaaaaa
    29461 gcaagggtga acgaagtaaa aaagaagtct gctaaggccc tgtctttgca cgggtaaaat
    29521 tttatatata aaaagaagct gggactaaag agctcagctt cctttggttt atataattgt
    29581 cattacaaga cgaagtggtt gggcgaaact ctgttgactt tattcaattt agagtttctt
    29641 atgcacaatt gagtctggaa cgaaagtctc cagttgcaaa gtatacagta caataaacca
    29701 acgatgtaat agctgatgac acaaagcaca gtgggtagga cttgcgaagt cacccttttc
    29761 ttttcaaaat ttatactaaa tcattgatat cagtgtagtc acgattaagt ccttgagcaa
    29821 ctggtaggct agtcaagtaa ccttgataag tggtcacacc ttgacgcaag ccttcatctt
    29881 cagagattgc ttgtgcgaat cctttgccag ccaaagcttc gatataagga agagtgacat
    29941 tggttagggc gatggttgaa gtgcgggcaa ccgcaccagg gatattggca acggcatagt
    30001 ggagaacacc gtgtttttca tagacgggtt catcgtgcgt tgtcacacgg tcagctgttt
    30061 cgataaggcc accttggtca acagcaatgt caacgataca gagcctggac gcatttgttt
    30121 gaccatctca tctgtcacca attccggtgc ttttgcacca gggatgagaa tggctccaat
    30181 caccacatca gcatctctca cacttgcttc aatgttgaat gaattagaca taagagtttg
    30241 aatttgactt ccaaagactt cttctagaac tgagagacgc ttggcactaa tatctaaaat
    30301 agtcacttga gcaccaagac caagggcgat gcgggcagca tgtgtaccga cgacaccatc
    30361 accgatgata gttacttttc ttttggaaca cctggtacac caccaggtag aacaccagag
    30421 ccaccagctt gcttagtaag gaagtgagct ccgatttaaa cagccatacg acctgcaacc
    30481 tcactcatag gaacgaggag cggtagttgt ccttgattgt cacgaacagt ttcataggca
    30541 attcctgttg tttttgctgc taacatagca tctgctaatt ctggagcagc ggccatgtgc
    30601 aagtaggtga agagaagaag atcgtcgcgc aagtaaccgt attcagaatt taaaggttct
    30661 tttactttca caaccaactc tgctgcccaa gcttcaccag cagtagcgac aatctcagct
    30721 ccttgctttt gatagtcagc atcagtaaag ccagaaccga gaccagcatt tgtttcgata
    30781 aggacacgat gaccacgact aactaagcta tgaacacctg caggtgtgag ggcgacacgg
    30841 ttttcgttat ttttaatttc ttttgggatt ccgattaaca ttgagataac ctacctttca
    30901 attgacggtc ttgttttggt tgtcacattc cagttcataa atcaaaaatg tgacggtttc
    30961 attgtatatg aaaccgcttc aaaaatcaag aaaaacttgt catccaaatt tttttatgct
    31021 agactagtga aaatcaagct ctaatggagg gaaaagtatg gaatcaattt ttgtaaaact
    31081 tgcccagtat ccgtctatag aaacggagcg tttattgctt agacctgtaa ctttggatga
    31141 tgcggaagca atgtttgact atgcctcgga caagggtaat acacgttaca cttttccaac
    31201 caatcaaagt ttggaagaaa ccaagaataa cattgctcag ttctacttgg ctaatccctt
    31261 gggacgttgg gggattgaat taaaagataa cggcaagttt atcggaacta ttgacttaca
    31321 caagattgat cctgttctta agaaggcagc tattggctac attatcaata aaaagtattg
    31381 gaatcaagga ttaacgacag aagccaatcg tgctgtgatt gagctagctt ttgagaagat
    31441 agggatgaat aagttgactg cccttcacga taaggacaat cctgcgtcag gaaaggtcat
    31501 ggagaaatca ggcatgcgtt tttcccatgc agaaccatat gcttgtatgg accagcatga
    31561 aaaaggccga atcgtgacaa gagttcatta tgtcttgacc aaggaagact attttgcaaa
    31621 taaataagca gttgaaaaga aatttttcga ctgttttttc ttcctcttac gaataatcta
    31681 agagaggaga aaatatggaa gcaattatcg agaaaatcaa agagtataaa atcatcgtca
    31741 tctgtactgg tctgggcttg cttgtaggag gatttttcct gctaaaacca gctccacaaa
    31801 cacctgtcaa agagacgaat ttgcaggctg aagtcgcagc tgtttccaag gatttggtat
    31861 ccgaagagga agtgaacaag gaagaaaagg aagaacccct tgaacaagat ctaatcacag
    31921 tagatgtcaa aggtgctgtc aaatcgccag ggatttatga cttgcctgta ggtagtcgag
    31981 tcaatgatgc tgttcagaag gctggtggct tgacagagca agcagacagc aagtcgctca
    32041 atctagctca gaaagttagt gatgaggctc tggtttacgt tcctactaag ggagaagaag
    32101 cagttagcca acagactggt ttggggacag cttcttcaat aagcaaggaa aagaaggtca
    32161 atctcaacaa ggccagtctg gaagaactca agcaggtcaa gggactggga ggaaaacgag
    32221 ctcaggacat tatcgaccat cgtgaggcaa atggcaagtt caagtcagta gacgagctca
    32281 agaaggtctc tggcattggt ggcaaaacaa tagaaaagct taaagactat gttacagtgg
    32341 attaagaatt tctctattcc cctaatttac ctgagttttc tattactttg gctttattac
    32401 gctattttct cagcatctta tcttgctttg ttgggctttg tttttctgct agtctgtctc
    32461 tttatccaat ttccgtggaa atctgctggt aaagttctaa taatttgcgg aatctttgga
    32521 ttttggtttg tttttcaaaa ttggcaacag agtcaagcga gtcaaaatct ggcggattct
    32581 gttgaaaggg tacggatttt acctgatact attaaggtta atggtgatag tctatccttt
    32641 cgtggcaagt ctaacggtcg tgctttccaa gtctattata aactccagtc cgaggaggag
    32701 aaagaagcct ttcaagcttt aaccgacctg catgagatag gactagaagg gaagctttcg
    32761 gagccagaag ggcagagaaa ttttggtggc tttaattacc aagcctatct gaagactcag
    32821 ggaatttacc agactctcaa tatcaaaaaa atccagtcac ttcaaaagat tggcagttgg
    32881 gatataggag aaaacttgtc cagtttacgt cgaaaggctg tggtttggat taagacgcac
    32941 tttccagacc ctatgcgcaa ttacatgaca ggactcttgc tgggatatct gaacaccgac
    33001 tttgaggaga tgaatgagct ttattccagt ctaggaatta tccacctctt tgccctatct
    33061 ggcatgcagg taggtttttt catgaatgga tttaagaaac ttctcttgcg attgggcttg
    33121 acccaagaaa agttgaaatg gctgacttat cccttttccc ttatctatgc gggactaatt
    33181 ggattttcag catcggttat tcgcagtctc ttgcaaaagc tactggctca acatggggtt
    33241 aagggcttgg ataattttgc cttgacggtg cttgtcctct ttattgtcat gccaaacttt
    33301 ttcttgacag caggaggagt cttgtcctgc gcttatgctt ttatcctgac catgaccagc
    33361 aaagaagggg aggggctcaa ggctgttgct agtgaaagtc tagtcatctc cttgggcata
    33421 ttgcccattc tatccttcta ttttgcggaa tttcaacctt ggtctatcct tttgaccttt
    33481 gtcttttcct ttctatttga cttgaccttc ttaccgctct tgtctatttt atttgtcctt
    33541 tcctttctct atccagtcat tcagctgaac tttatctttg aatggttaga gggcattatt
    33601 cgcttggtct cgcaggtggc aaggagaccg cttgtctttg gtcaacccaa cgcatggctt
    33661 ttaatcttat tgttaatttc cttggctttg gtctatgatt tgaggaaaaa cattaaagga
    33721 ttaacagtat tgagtttatt gattacaggt ctctttttcc ttaccaagta tccactggaa
    33781 aatgaaatca ccatgctgga tgtggggcaa ggagaaagta ttttcctacg ggatgtaact
    33841 gggaaaacca ttctcataga tgtaggtggt aaggcagaat cttataagaa aatcgaaaaa
    33901 tggcaagaaa agatgacgac cagcaatgca cagagaagct tgatacccta tcttaaaagt
    33961 cgaggagtag ctaagattga ccagctgatt ttgacaaata cggacaagga acatgtcgga
    34021 gatttgttag aggtgaccaa ggctttccat gtaggggaga ttttagtatc aaaaggcagt
    34081 ttgaagcaga agcaatttgt ggtagaacta caggcgactc aaacaaaggt gcgtagtatg
    34141 acagtagggg agaacttgcc catttttgga agtcagttag aagttctatc cccaaggaaa
    34201 atgggagatg gaggacacga tgatacccta gttctgtatg ggaaattctt ggataagcaa
    34261 tttctcttca cgggaaattt ggaggagaaa ggagagaagg acttgctgaa gcactatcca
    34321 gacttgaaag taaatgtttt gaaagctagc caacatggca ataaaaaatc atcaagtcca
    34381 gcctttctag aaaaactcaa accagagctt actcttatct cagttggaaa gagcaatcga
    34441 atgaaactcc cccatcagga aacattgaca cgactggaag gtatcaatag caaagtttac
    34501 cgaactgacc agcaaggagc tatacgcttt aaggggttgg atagttggaa aatcgaaagt
    34561 gttcgatagg aaagataaat gttgtagatt agtgaaataa actaaaaatt tgttgcataa
    34621 taatgataaa aatggtataa tgaaaacgta ttcaatattg aggatataaa atcattaaaa
    34681 attagcaaaa atcgtttaat tagttagttt ataatctatt ggtcttcttc agtccagtgt
    34741 atctgctgtg acagtcacta aaagttacaa gtatgattgg aatacggttt gggaatatag
    34801 taccaactat cacggccatc agtatgcttg gattccgtca tggtctcgtt atgacagcta
    34861 ttctgagtat aaagttggcg gaggctggaa ctacggtcgt tatgaggtca taaactatta
    34921 cagcggaggc tattaattct taaagagtga gaaaaaggag ggctagatat gttgcagctt
    34981 actcatgtga ccttaaaaac gcgacaagtc atcttgcaag atgcggattt tacctttaaa
    35041 aagggtagga tttatggcct tcttgctatc aatggctcgg gaaagacgac actattccga
    35101 gctatgagca agttgcttcc ccttagtagt ggacacatcg cagttcctcc ttctttgttt
    35161 tattatgaga gcgttgaatg gctggatgga aacttaagtg ggatggacta ccttcgtctc
    35221 ataaaaaaca tctggaagtc agacctaaac ttgagagatg aaatcgccta ctgggaaatg
    35281 gctgactata tcagtcttcc catccgcaag tattccttag gcatgaagca acgcttggtg
    35341 attgctatgt attttctcag tcaggccaaa tgctggctca tggatgagat tacaaatggc
    35401 ttagacgagt attatcgaca gaagtttttt gataggctag cacaaatcga tagacaagaa
    35461 cagctggttc ttttaagttc ccactataag gaagagttgg ttgatatctg cgatagagta
    35521 gtaaccattc atcaggggca gatagaagag gtttagttta tgaaagatgt tagtctattt
    35581 ttattgaaaa aagttttcaa aagtcgttta aactggatta tcttactttt atttgcatct
    35641 gtactcggtg ttacctttta tttaaatagt cagactgcaa actcacacag cttggagagc
    35701 aggttggaaa gtcgcattgc agccaacgag agggctatca atgaaaatga agagaaactc
    35761 tcccaaatgt ctgataccag ctcggaggaa taccagtttg ctaaaaataa tttagacgtg
    35821 caaaaaaatc ttttgacgcg aaagacagaa attctaactt tactaaaaga agggcgctgg
    35881 aaagaagcct actatctgca gtggcaagat gaagagaaga attatgaatt tgtatcaaat
    35941 gacccgactg ctagctctgg cttaaaaatg ggggttgacc gcgaacggaa gatttaccaa
    36001 gccctgtatc ccttgaacat aaaagcacat actttggagt ttccgaccca cgggattgat
    36061 cagattgtct ggattttaga ggttatcatc ccaagcttgt ttgtggttgc gattattttt
    36121 atgctaacac aactatttgc agaaagatat caaaatcatc tggacacagc tcacttatat
    36181 cctgtttcaa aagtgacatt tgcaatatcc tctcttggag ttggagtggg atatgtaact
    36241 gtgctgttta tcggaatctg tggcttttct tttctagtgg gaagtctgat aagtggtttt
    36301 ggacagttag attatcccta cccaatttat agcttagtga atcaagaagt aactattggg
    36361 aaaatacaag atgtattatt tcctggcttg ctcttagctt tcttagcctt tatcgtcatt
    36421 gtggaagttg tgtacttgat tgcttacttt ttcaagcaaa aaatgcctgt cctctttctt
    36481 tcactcattg ggattgttgg cttattgttt ggcatccaaa ccattcagcc tcttcaaagg
    36541 attgcacatc tgattccctt tacttacttg cgttcagtgg agattttatc tggaagatta
    36601 cctaagcaaa ttgataatgt caatctaaat tggagcatgg gaatggtctt acttccttgc
    36661 ctgattatct ttttgctagt ggggattcta tttattgaaa gctggggaag ttcacgtaaa
    36721 aaggaagttt ttaataggtc ttagcattct tataaggaag ttaaggaaaa actaacatag
    36781 agagggaatc aacttgattc tctctttttg attcgaaaac caaaccaaaa tacaaacaca
    36841 aacttttcaa aaaaataact ttttatcttg acaagagcta gaaaacttgg tatcatttaa
    36901 aagttgagaa aagcagaagt gagagcttct cgccttgtga cattaagttg cctggcccta
    36961 cggatgaaaa gtttcgaaga aacgctatca taacgtgcgg gcttgtatat ttacgagtcc
    37021 gctattgttt ttctctaata aaacaaaaga ggtgaaaacc atagcaaagc aagacttatt
    37081 catcaatgat gagattcgtg tacgtgaagt tcgcttgatt ggtcttgagg gagaacagct
    37141 aggcatcaag ccactcagtg aagcgcaagc tttggctgat aacgctaatg ttgacctagt
    37201 attgattcaa ccccaagcca aaccgcctgt tgcaaaaatt atggactacg gtaagttcaa
    37261 atttgagtac cagaagaagc aaaaagaaca acgtaaaaaa caaagcgttg ttactgtgaa
    37321 agaagttcgt ctaagtccaa caattgacaa gggtgacttt gatacaaaac ttcgtaatgc
    37381 acgtaagttc cttgaaaaag gaaataaagt taaggtatct attcgcttta agggtcgtat
    37441 gattacccat aaagagattg gtgcaaaagt tttagccgag tttgctgaag caactcaaga
    37501 tattgccatc atcgaacaac gtgctaaaat ggatggacgt caaatgttca tgcaattggc
    37561 gccagcgact gacaaaaaat aattgtcaga aagttaaata aaggagaaaa aatcatgcca
    37621 aaacaaaaaa cacaccgcgc atcagctaaa cgtttcaaac gtacaggttc tggtggactt
    37681 aaacgtttcc gtgcttacac ttctcaccgt ttccacggaa aaactaagaa acaacgtcgt
    37741 catcttcgta aagcatctat ggtgcattca ggagattaca aacgtatcaa agcaatgctt
    37801 actcgcttga aataatagct tgactgtaag taaacaattc taggaaatat ttggaggaaa
    37861 taaaacatgg cacgtgttaa aggtggcgtt gtatcacgca aacgtcgtaa acgtattctt
    37921 aaattagcaa aaggttacta tggagctaaa cacatcttgt tccgtactgc aaaagaacaa
    37981 gtaatgaact cttactacta tgcataccgt gaccgtcgtc agaaaaaacg tgacttccgc
    38041 aaattgtgga ttactcgtat caatgcggca gctcgtatga acggactttc atactcacaa
    38101 ttgatgcatg gtttgaaatt ggctgagatc gaagttaacc gtaaaatgct tgctgacttg
    38161 gctgttaacg atgcagtagc tttcacagct cttgcagatg cagctaaagc aaaacttggt
    38221 aaataacaac tgatgagact ggaacaggat tgtccagtct ttttaaaaat aaaggagaaa
    38281 gatatggctt caaaaatgct acacacttgc ttacgagtag aaaatcttga aaaatcaatt
    38341 gcattttatc aagatgcttt tggttttaaa gaattgcgtc gcagagattt tccagaccat
    38401 gccttcacga ttgtctatct aggtcttgag ggtgatgatt atgagttgga gttgacttat
    38461 aactacgatc acggtccgta tgtggttggt gatgggtttg cccatatcgc cctcagtaca
    38521 cctgatcttg aggcacttca tcaagaacac agtgcaaaag gctatgaagt gactgagcca
    38581 aatggtctac caggaactac accaaactat tactttgtta aagaccctga tggatataag
    38641 gtcgaagtca ttcgtgaaaa ataatttcgt gcaagttaag tagaatattc gttttctact
    38701 tgactttttt tagctgaaag agtatactaa taaaaattca acctttaagg ggagtccaga
    38761 gagactcaca aggtgtcaga taaaagaata gtgcaatttt ctagaggaga ccttttgagt
    38821 gtgctctctt gtgttgtacg attttaactg aggccttgca ctagcaaggt cttttcttta
    38881 tctggtcccc ttaaaattta aggaggaaaa gttatgaatc tcacatgtaa gaagcgtttg
    38941 ggtgtcattc ggttggaaac catgaaggtg gttgcacaag aggaaatcgc gccagcaatc
    39001 tttgaattag tcctagaagg agaaatggtt gaagccatgc gagcaggcca atttcttcat
    39061 ctgcgtgtac cggacgatgc ccatctctta cgtcgtccta tttcaatttc gtctattgac
    39121 aaggcaaaca agcagtgtca cctcatttat cggattgagg gagctgggac tgcaattttt
    39181 tcaaccttaa gtcagggaga cactcttgat gtgatggggc ctcagggaaa tggttttgac
    39241 ttgtctgacc ttgatgagca gaatcaggtt ctccttgttg gtggtgggat tggtgttcca
    39301 cccttgcttg aggtggccaa ggaattgcat gaacgtggag tgaaagtagt gacagtcctc
    39361 ggttttgcta ataaggatgc tgttattttg aaaacggaat tggctcagta tggtcaggtc
    39421 tttgtaacga cagatgatgg ttcttatggc atcaagggaa atgtttccgt tgttatcaat
    39481 gatttagata gtcagtttga tgctgtttac tcgtgtgggg ctccaggaat gatgaagtat
    39541 atcaatcaaa cctttgatga tcacccaaga gcctatttat ctctggaatc tcgtatggct
    39601 tgtgggatgg gagcttgcta tgcctgtgtt ctaaaagtac cagaaagcga gacggtcagc
    39661 caacgcgtct gtgaagatgg tcctgttttc cgcacaggaa cagttgtatt ataaggagaa
    39721 aattatgact acaaatcgat tacaagtgtc tctacctggt ttggatttga aaaatccgat
    39781 tattccagca tcaggctgtt ttggctttgg acaagagtat gccaagtact atgatttaga
    39841 ccttttaggt tctattatga tcaaggcgac aacccttgaa ccacgttttg ggaatccaac
    39901 tccaagagtg gcagagacgc ctgctggtat gctcaatgca attggcttgc aaaatcctgg
    39961 tttagaggtt gttttggctg aaaagctacc ttggctggaa agagaatatc caaatcttcc
    40021 tattattgcc aatgtagctg gtttttcaaa acaagagtat gcagctgttt ctcatgggat
    40081 ttccaaggca actaatgtaa aagctatcga gctcaatatt tcttgtccca atgttgacca
    40141 ctgtaatcat ggacttttga ttggtcaaga tccagatttg gcttatgatg tggtgaaagc
    40201 agctgtggaa gcctcagaag tgccagttta tgtcaaatta accccgagtg tgaccgatat
    40261 cgttactgtc gcaaaagctg cagaagatgc gggagcaagt ggcttgacca tgatcaatac
    40321 tctggttgga atgcgctttg acctcaaaac cagaaaacca atcttggcca atggaacagg
    40381 tggaatgtca ggtccagcag tctttccagt agccctcaaa ctcatccgcc aagtagccca
    40441 aacaacagac ctgcctatca ttggaatggg gggagtggat tcggctgaag ctgccctaga
    40501 aatgtatctg gctggggcat ctgctatcgg agttggaaca gctaacttta ccaatcctta
    40561 tgcctgtcct gacatcatcg aaaatttacc aaaagtcatg gataaatacg gtattagcag
    40621 tctggaagaa ctccgtcagg aagtaaaaga gtctctgagg taaattgcaa tcaatctgtt
    40681 cttgattttt tattagtttg taatatgaat ttaggagaat tttggtacaa taaaataaat
    40741 aagaacagag gaagaaggtt aatgaagaaa gtaagattta tttttttagc tctgctattt
    40801 ttcttagcta gtccagaggg tgcaatggct agtgatggta cttggcaagg aaaacagtat
    40861 ctgaaagaag atggcagtca agcagcgaat gagtgggttt ttgatactca ttatcaatct
    40921 tggttctata taaaagcaga tgctaactat gctgaaaatg aatggctaaa gcaaggtgac
    40981 gactattttt acctcaaatc tggtggctat atggccaaat cagaatgggt agaagacaag
    41041 ggagcctttt attatcttga ccaagatgga aagatgaaac gaaatgcttg ggtaggaact
    41101 tcctatgttg gtgcaacagg tgccaaagta atagaagact gggtctatga ttctcaatac
    41161 gatgcttggt tttatatcaa agcagatgga cagcacgcag agaaagaatg gctccaaatt
    41221 aaagggaagg actattattt caaatccggt ggttatctac tgacaagtca gtggattaat
    41281 caagcttatg tgaatgctag tggtgccaaa gtacagcaag gttggctttt tgacaaacaa
    41341 taccaatctt ggttttacat aaaagaaaat ggaaactatg ctgataaaga atggattttc
    41401 gagaatggtc actattatta tctaaaatcc ggtggctaca tggcagccaa tgaatggatt
    41461 tgggataagg aatcttggtt ttacctcaag tttgatggca aaatggctga aaaagaatgg
    41521 gtctacgatt ctcatagtca agcctggtac tacttcaaat ccggtggtta catgacagcc
    41581 aatgaatgga tttgggataa ggaatcttgg ttttatctca aatctgatgg gaaaatagct
    41641 gaaaaagaat gggtctacga ttctcatagt caagcttggt actacttcaa atccggtggt
    41701 tacatgacag ccaatgaatg gatttgggat aaggaatctt ggttttacct caagtctgat
    41761 ggcaaaatgg ctgaaaaaga atgggtctac gattctcata gtcaagcctg gtactacttc
    41821 aaatctggtg gctacatggc gaaaaatgag acagtagatg gttatcagct tggaagcgat
    41881 ggtaaatggc ttggaggaaa agctacaaat aaaaatgctg cttactatca agtagtgcct
    41941 gtcacagcca atgtttatga ttcagatggt gaaaagcttt cctatatatc gcaaggtagt
    42001 gtcgtatggc tagataagga tagaaaaagt gatgacaagc gcttggctat tactatttct
    42061 ggtttgtcag gctatatgaa aacagaagat ttacaagcgc tagatgctag taaggacttt
    42121 atcccttatt atgagagtga tggccaccgt ttttatcact atgtggctca gaatgctagt
    42181 atcccagtag cttctcatct ttctgatatg gaagtaggca agaaatatta ttcggcagat
    42241 ggcctgcatt ttgatggttt taagcttgag aatcccttcc ttttcaaaga tttaacagag
    42301 gctacaaact acagtgctga agaattggat aaggtattta gtttgctaaa cattaataat
    42361 agccttttgg agaacaaggg cgctactttt aaggaagccg aagaacatta tcatatcaat
    42421 gccctttatc tccttgccca tagtgcccta gaaagtaact ggggaagaag taaaattgcc
    42481 aaagataaga ataatttctt tggcattaca gcctatgata cgacccctta cctttctgcc
    42541 aagacatttg atgatgtgga taagggaatt ttaggtgcaa ccaagtggat taaggaaaat
    42601 tatatcgata ggggaagaac tttccttgga aacaaggctt ctggtatgaa tgtggaatat
    42661 gcttcagacc cttattgggg cgaaaaaatt gctagtgtga tgatgaaaat caatgagaag
    42721 ctaggtggca aagattagta ctataagtga atatgatttg agtgaatagt aagttaaaaa
    42781 tcctgatttc aagtaaaatc aggatttttt catggatgca atttttttgg agtctggtgt
    42841 gacgcggagg gtcttttgtc ctgtgtaagt gacaaagccg ggttttccac cagttggttt
    42901 attgagtttt ttgacttcaa tcatatctac ctgcaccaga ttcgacaggc gcccttgaga
    42961 gaagtaggca gctaactctg ctgcgtctgt cttgactgca tcagatgggt caagatttcc
    43021 tgagatgaca acatggcttc caggaatgtc cttagcatgg aaccaaagtt cctccttgcg
    43081 ggccatttta aaggtcaatt cctcattttg aagattgttt cgtccgacat agatgatggt
    43141 tttgccatcg cttgctagat attgttctag ttttttgcgt ttctggattt tctcccgttg
    43201 tcttctgcgg ataaaacctg tttgaatcaa ttcttcacgg atttcagcga tttcttccag
    43261 tccagcttgg ttgaggacgg tttctacact ttccagatag agaatagtgg ctttggtttc
    43321 ttcaatcaaa tcagtcaagt atttgacagc ttctttgagt ttctgatacc gtttaaaata
    43381 gcgttgggca ttctggttgg gagtcagagc cttatcaagc gcaatcatga taggttggtt
    43441 ggtatagtag ttgtctagga taacctggtc ttggtcgtta ggcacttggt gtaggaaggt
    43501 tgtcagcaat tctccttttt gacgaaattc ttcagcgttg tctgtcgcca gtaactcttt
    43561 ttcctgtttt ttgagtttgt gtcggttttt ctgaagttca ttttcaacac gacgaatcag
    43621 ttcactggcc tgctgtttga cgcggtcgcg ctcagcctta tccttatagt aggtgtccaa
    43681 caaatcagaa agatttgcaa aaggctctcc cacctgattt gcaaaaggaa ctggactgaa
    43741 ggaagtctca gtcaagcatg gcttggtttc ttgattgaaa aaatttcgga aagcggaaag
    43801 tttttcacta accagtatcc tttccaattc atttgcagta tcgcgtccca gaccttgaaa
    43861 gaggctttga agattttttg ctgttagttc ttgggtttgc aggatttcaa agagcttttc
    43921 atctttgata gtaaaaggat tgagagattc tgtacttggc ggagcgatat aggtcgatcc
    43981 tggaagtaag gtgcggtagc tattttgtga aaaaccgacg tgtttgataa cttcgaggat
    44041 tttatgactg cttttatcga ccagtagaat attactgtgt ttccccataa tttcaataat
    44101 caaggtagcc tggatatggt ctccaatctc gtttttattg gaaactgtaa tttccacaat
    44161 acggtcattt tccacttgct caatcgactc aatcagggcc ccctgcaaat actttctcaa
    44221 aaccatgata aaggtagaag gttgagctgg attttcaaaa gtcgtttggg tcagctgaat
    44281 gcgtccaaaa actggatggg cagaaaggag caggcgatgg ctttggcgat tgctgcggat
    44341 ttgcaagacc aactcttgtt caaaaggctg attgattttc tggatgcgac cattcactaa
    44401 ttcgcttcgc aattcctcaa ctatgtggtg taaaaaaaat ccatcaaatg acatcgttct
    44461 ctccttgtga ttgtattcca tagtattata tcaaaaaggt agaataaaat catagaaatg
    44521 tggtataata aagccaagta aagagaaacg agaagcacat gtatattgaa atggtagatg
    44581 aaactggtca agtttcaaaa gaaatgttgc aacaaaccca agaaattttg gaatttgcag
    44641 cccaaaaatt aggaaaagaa gacaaggaga tggcagtcac ttttgtgacc aatgagcgta
    44701 gtcatgaact taatctggag taccgtgaca ccgaccgtcc gacagatgtc atcagccttg
    44761 agtataaacc agaattggaa attgcctttg acgaagagga tttgcttgaa aatccagaat
    44821 tggcagagat gatgtctgag tttgatgcct atattgggga attgttcatc tctatcgata
    44881 aggctcatga gcaggccgaa gaatatggtc acagctttga gcgtgagatg ggcttcttgg
    44941 cagtacacgg ctttttacat attaacggct atgatcacta cactccggaa gaagaagcgg
    45001 agatgttcgg tttacaagaa gaaattttga cagcctatgg actcacaaga caataaacga
    45061 aaatggaaaa atcgtgacct gatatccagt ttagaatttg ccatcacagg gatttttact
    45121 gctatcaagg aagaacgaaa tatgcgaaaa catgcagtga cggctctagt ggtcatcctt
    45181 gcaggttttg tttttcaggt gtcacgaatc gaatggctct ttctcctatt gagcattttc
    45241 ttggtagtag cctttgagat tatcaactct gctattgaaa atatggtgga tttggccagt
    45301 cactatcact tttccatgct ggctaaaaat gccaaggata tggcggctgg cgcggtatta
    45361 gtggtttctc ttttcgcagt cttaacaggt gcattgattt ttctcccacg aatctgggat
    45421 ttattatttt aaacagtaag aggaaattat gacttttaaa tcaggctttg tagccatttt
    45481 aggacgtccc aatgttggga agtcaacctt tttaaatcac gttatggggc aaaagattgc
    45541 catcatgagt gacaaggcgc agacaacgcg caataaaatc atgggaattt acacgactga
    45601 taaggagcaa attgtcttta tcgacacacc agggattcac aaacctaaaa cagctctcgg
    45661 agatttcatg gttgagtctg cctacagtac ccttcgcgaa gtggacaccg ttcttttcat
    45721 ggtgcctgct gatgaagcgc gtggtaaggg ggacgatatg attatcgagc gtctcaaggc
    45781 tgccaaggtt cctgtgattt tggtggtgaa taaaatcgat aaggtccatc cagaccagct
    45841 cttgtctcag attgatgact tccgtaatca aatggacttt aaggaaattg ttccaatatc
    45901 agcccttcag ggaaataacg tgtctcgtct agtggatatt ttgagtgaaa atctggatga
    45961 aggtttccaa tatttcccgt ctgatcaaat cacagaccat ccagaacgtt tcttagtttc
    46021 agaaatggtt cgcgagaaag tcttgcacct aactcgtgaa gagattccgc attctgtagc
    46081 agtagttgtt gactctatga aacgagacga agagacagac aaggttcaca tccgtgcaac
    46141 catcatggtc gagcgcgata gccaaaaagg gattatcatc ggtaaaggtg gcgctatgct
    46201 taagaaaatc ggtagcatgg cccgtcgtga tatcgaactc atgctaggag acaaggtctt
    46261 cctagaaacc tgggtcaagg tcaagaaaaa ctggcgcgat aaaaagctag atttggctga
    46321 ctttggctat aatgaaaaag aatactaagt agaggtgggc tcatgcctgc ttcttgtttt
    46381 tacagaagga ggacttatgc ctgaattacc tgaggttgaa accgtttgtc gtggcttaga
    46441 aaaattgatt ataggaaaga agatttcgag tatagaaatt cgctacccca agatgattaa
    46501 gacggatttg gaagagtttc aaagggaatt gcctagtcag attatcgagt caatgggacg
    46561 tcgtggaaaa tatttgcttt tttatctgac agacaaggtc ttgatttccc atttgcggat
    46621 ggagggcaag tatttttact atccagacca aggacctgaa cgcaagcatg cccatgtttt
    46681 ctttcatttt gaagatggtg gcacgcttgt ttatgaggat gttcgcaagt ttggaaccat
    46741 ggaactcttg gtgcctgacc ttttagacgc ctactttatt tctaaaaaat taggtcctga
    46801 accaagcgaa caagactttg atttacaggt ctttcaatct gcccttgcca agtccaaaaa
    46861 gcctatcaaa tcccatctcc tagaccagac cttggtagct ggacttggca atatttatgt
    46921 ggatgaggtt ctctggcgag ctcaggttca tccagctaga tcttcccaga ctttgacagc
    46981 agaagaagcg acagctattc atgaccaaac cattgctgtt ttgggccagg ctgttgaaaa
    47041 aggaggttcc accattcgga cttataccaa tgcctttggg gaagatggaa gcatgcagga
    47101 ctttcatcag gtctatgata agactggtca agagtgtgta cgctgtggta ccatcattga
    47161 gaaaattcaa ctaggcggac gaggcaccca cttttgtcca aactgtcaaa ggagggactg
    47221 atgagaaaaa tcatcggaat cactggggga attgcctctg gtaagtcaac tgtgacaaat
    47281 tttctaagac agcaaggctt tcaagtagtg gatgccgacg cagtcgtcca ccaactacag
    47341 aaacctggtg gtcgtctgtt tgaggctcta gtacagcact ttgagcaaga aatcattctt
    47401 gaaaacggag aactcaatcg ccctctccta gctagtctca tcttttcaaa tcctgaagag
    47461 caaaaatggt cgaagcaaat tcaaggggag attatccgtg aggaactggc tactttgaga
    47521 gaacagttgg ctcagacaga agagattttc ttcatggata ttcccctact ttttgagcag
    47581 gactacagcg attggtttgc tgagacttgg ttggtctatg tggaccgaga tgcccaagta
    47641 gaacgcttaa tgaaaaggga ccagttgtcc aaagatgaag ctgagtctcg tctggcagcc
    47701 cagtggcctt tagaaaaaaa gaaagatttg gccagccagg ttcttgataa taatggcaat
    47761 cagaaccagc ttcttaatca agtgcatatc cttcttgagg gaggtaggca agatgacaga
    47821 gattaactgg aaggataatc tgcgcattgc ctggtttggt aattttctga caggagccag
    47881 tatttctttg gttgtacctt ttatgcccat cttcgtggaa aatctaggtg tagggagtga
    47941 gcaagtcgct ttttatgcag gcttagcaat ttctgtctct gctatttccg cggcgctctt
    48001 ttctcctatt tggggtattc ttgctgacaa atacggccga aaacccatga tgattcgggc
    48061 aggtcttgct atgactatca ctatgggagg cttggccttt gtcccaaata tctattggtt
    48121 aatctttctt cgtttactaa acggtgtatt tgcaggtttt gttcctaatg caacggcact
    48181 gatagccagt caggttccaa aggagaaatc aggctctgcc ttaggtactt tgtctacagg
    48241 cgtagttgca ggtactctaa ctggcccctt tattggtggc tttatcgcag aattatttgg
    48301 cattcgtaca gttttcttac tggttggtag ttttctattt ttagctgcta ttttgactat
    48361 ttgctttatc aaggaagatt ttcaaccagt agccaaggaa aaggctattc caacaaagga
    48421 attatttacc tcggttaaat atccctatct tttgctcaat ctctttttaa ccagttttgt
    48481 catccaattt tcagcccaat cgatcggccc tattttggct ctttatgtac gcgacttagg
    48541 gcagacagag aatcttcttt ttgtctctgg tttgattgtg tccagtatgg gcttttccag
    48601 catgatgagt gcaggagtca tgggcaagct aggtgacaag gtgggcaatc atcgtctctt
    48661 ggttgtcgcc cagttttatt cagtcatcat ctatctcctc tgtgccaatg cctctagccc
    48721 ccttcaacta ggactctatc gtttcctctt tggcttggga accggtgcct tgattcccgg
    48781 ggttaatgcc ctactcagca aaatgactcc caaagccggc atttcgaggg tctttgcctt
    48841 caatcaggta ttcttttatc tgggaggtgt tgttggtccc atggcaggtt ctgcagtagc
    48901 aggtcaattt ggctaccatg ctgtctttta tgcgacaagc ctttgtgttg cctttagttg
    48961 tctctttaac ctgattcaat ttcgaacatt attaaaagta aaggaaatct agtgcgagta
    49021 aaaattaatc tcaaatgctc ctcttgtgac agtatcaatt acctaaccag taaaaattca
    49081 aaaacccatc cagacaagat tgaggtttta aagtattgtc ccaaggaaag aaaagtaacc
    49141 ctacatcttg aatctaagta gattttatgg taaaataata gggattttaa ggagtttgat
    49201 atgtataacc tattattaac cattttatta gtattatctg ttgtgattgt gattgcaatt
    49261 ttcatgcaac caaccaaaaa ccaatccagc aatgtatttg atgccagttc aggtgatttg
    49321 tttgaacgca gtaaagctcg cggttttgaa gctgtaatgc agcgtttgac agggatttta
    49381 gtctttttct ggctagccat tgccttagca ttgacggtat tatcaagtag ataagaaaat
    49441 aatgggcagg actaggtctt tgcctctttt tatttttaaa ggatgtttga gaaggtttta
    49501 cagtaaaaga aaattaaaaa atctagaaag aaaatatgaa agatagaata aaagaatatt
    49561 tacaagacaa gggaaagatg actgttaatg atttggctca ggctttggga aaagacagtt
    49621 ccaaggattt tcgtgagttg attaaaacct tgtccttaat ggaaagaaag caccaaattc
    49681 gttttgaaga agatggtagt ctgacattag aaattaagaa aaaacatgag attaccctca
    49741 aggggatttt tcatgcccat aaaaatggct ttggctttgt tagtctggaa ggcgaggagg
    49801 acgacctttt tgtagggaaa aatgatgtca actatgctat tgatggtgat accgtcgagg
    49861 tagtgattaa gaaagtcgct gaccgcaata agggaacagc agcagaagcc aaaattattg
    49921 atatcctaga acacagtttg acaacagttg tcgggcaaat cgttctggat caggaaaagc
    49981 ctaagtatgc tggctatatt cgttcaaaaa atcagaaaat cagtcaaccg atttatgtta
    50041 agaaaccagc cctaaagtta gaaggaacag aagttctcaa ggtctttatc gataaatacc
    50101 caagcaagaa acatgatttc tttgtcgcga gtgttctcga tgtagtggga cactcaacgg
    50161 atgtcggaat tgatgttctt gaggtcttgg aatcaatgga cattgtatcc gagtttccag
    50221 aagctgttgt taaggaagca gaaagtgtgc ctgatgctcc gtctcaaaag gatatggaag
    50281 gtcgtctgga tctaagagat gaaattacct ttaccattga cggtgcggat gccaaggact
    50341 tggacgatgc agtgcatatc aaggctctga aaaatggcaa tctggagctt ggggttcaca
    50401 tcgcagatgt ttcttattat gtgaccgagg ggtctgccct tgacaaggaa gcccttaacc
    50461 gtgcgacttc tgtttacgtg acagaccgag tggtgccaat gcttccagaa cgactatcaa
    50521 atggcatctg ctctctcaat ccccaagttg accgcctgac ccagtctgct attatggaga
    50581 ttgataaaca tggtcgtgtg gtcaactata ccattacaca aacagttatc aagaccagtt
    50641 ttcgtatgac ctatagcgat gtcaatgata tcctagctgg cgatgaagaa aagagaaaag
    50701 aatatcataa aattgtatta agtatcgaac tcatggccaa gcttcatgaa actttagaaa
    50761 acatgcgtgt gaaacgtgga gctctcaatt ttgataccaa tgaagcgaag attttagtgg
    50821 ataaacaagg taagcctgtt gatatcgttc ttcggcagcg tggtattgcc gagcggatga
    50881 ttgagtcttt tatgttgatg gctaatgaaa cagttgccga acatttcagc aagttggatt
    50941 tgccttttat ctatcgaatt cacgaggagc ctaaggctga aaaggttcag aagtttattg
    51001 attatgcttc gagttttggc ttgcgcattt atggaactgc cagtgagatt agtcaggagg
    51061 cacttcaaga catcatgcgt gctgttgagg gagaacctta tgcagatgta ttgtccatga
    51121 tgcttcttcg ctctatgcag caggctcgtt attcggagca caatcacggc cactatggac
    51181 tagctgctga ctattatact cactttacca gtccaattcg tcgttatcca gaccttcttg
    51241 ttcaccgtat gattcgagat tacggctgtt ctaaggaaat agcagagcat tttgaacaag
    51301 tgattccaga gattgcgacc cagtcttcca accgtgaacg tcgtgccata gaagctgagc
    51361 gtgaagtcga agccatgaaa aaggctgagt atatggaaga atacgtgggt gaagagtatg
    51421 atgcagttgt atcaagtatt gtcaaattcg gtctctttgt cgaattgcca aacacagttg
    51481 aaggcttgat tcacatcact aatctacctg aattttatca tttcaatgag cgtgatttga
    51541 ctcttcgtgg agaaaaatca ggtatcactt tccgagtggg tcagcagatc cgtatccgtg
    51601 ttgaaagagc ggataaaatg actggagaga ttgatttttc attcgtacct agtgagtttg
    51661 atgtgattga aaaaggcttg aaacagtcta gtcgtagtgg cagagggcgt ggttcaaatc
    51721 gtcgttcgga taagaaggaa gacaagagaa aatcaggacg ctcaaatgat aagcgtaagc
    51781 attcacaaaa agacaagaag aaaaaaggaa agaaatcttt ttacaaggaa gtagctaaga
    51841 aaggagccaa gcatggcaaa gggcgaggga aaggtcgtcg cacaaaataa aaaggcacgc
    51901 cacgactata caatcgtaga tacgctagag gcagggatgg tcctgactgg aactgaaatc
    51961 aagagtgtac gagctgctcg aattaatctc aaggatggct ttgctcaagt gaaaaatgga
    52021 gaagtttggc tgagcaatgt tcatatcgcg ccttacgaag agggcaatat ctggaaccag
    52081 gaaccagaac gtcgtcgtaa actcctgctc cataaaaagc aaattcaaaa attggaacaa
    52141 gagaccaaag ggacaggaat gaccttagtt ccccttaagg tctatataaa agatggctac
    52201 gctaagcttc ttttaggact tgccaaaggg aagcatgact atgacaaacg ggagtctatc
    52261 aaacgtcgtg agcaaaatcg agatatcgcg cgtgtgatga aagctgttaa tcagcgataa
    52321 aaagaggaat tgaaaatgga aaaattagtt gcctataaac gcatgccttt gtggaataaa
    52381 caaacaatgc ctgaagctgt tcagcaaaag cacaatacaa aagttgggac ttgggggaaa
    52441 attactgtct tgaagggagc tctcaagttt attgaattga cagaagaagg ggaagttcta
    52501 gctgaacacc tctttgaagc aggggcagac aatccaatgg cccaacctca agcctggcac
    52561 cgagtggaag ctgccacaga tgatgtggaa tggtacttgg aattttattg taaacctgag
    52621 gattattttg ctaaaaaata caataccaat cctgttcatt cagaggtcct agaggccatg
    52681 cagacagtga aacaagggaa agctttggat ttgggttgtg gtcaggggcg taattctctt
    52741 tttctagccc agcaagattt tgatgtgacg gctgtagatc aaaatggact agctcttgaa
    52801 atcttgcaaa gcattgtgga gcaggaagat ttggacatgc ctgttggcct ttacgatatc
    52861 aattcagcta gcattgaaca agaatatgat tttatcgttt caacagttgt tctcatgttt
    52921 ctacaagcgg accgcattcc agctattatt caaaatatgc aggagaaaac cagtgttggc
    52981 ggttacaacc ttatcgtttg tgccatggac acggaggatt atccttgctc ggttaacttc
    53041 ccattcacct ttaaagaagg agaactggca gactattaca aggattggga attggttaag
    53101 tacaatgaaa atccaggcca tttgcaccgt cgcgatgaga atggcaatcg tattcaacta
    53161 cgctttgcga ccttactagc taagaaaatc aagtaaacac acatgaagat taggaatttt
    53221 cctgatcttt tttctttttt acgaatgata tagaaaagga gggaattcat gtttgttgcg
    53281 agagatgcta ggggagaatt ggtaaatgtg ttagaggata aacttgagaa gcaagcatac
    53341 acctgcccag cttgtggagg ccagctccat ttgcgtcaag gaccaagtgt acggacgcat
    53401 tttgcccata aatccttaaa agactgtgat tttttctttg aaaatgaaag tccagaacac
    53461 ctggccaata aggaatccct ctatcactgg ttgaaaaaag agacaaaggt tcaattagag
    53521 tacccgcttt cagaacttaa acagattgcg gatgtatttg taaatggcaa tctagctcta
    53581 gaagttcagt gtagtccctt gcctcagaaa gtccttaaag agcgaagtga gggctatcgt
    53641 agtcagggtt accaagtact gtggttgctg ggtcaaaaac tgtggctcaa ggagcgtttg
    53701 actcgtctac agcaaggttt tctttatttc agtcaaaaca tgggctttta tgtttgggaa
    53761 ttagacaagg aaaaacaagt tttaagactc aaatacctga tttaccagga tctccgcggt
    53821 aaactccatt atcaaatcaa ggaattttcc tatggtcaag gtagtttatt ggaaatattg
    53881 cgtcttccct ataagagaca aaaaatatct cattttacag tttctgagga caaggacatc
    53941 tgtcgctata tccggcaaca actttattat caaaatctct tttggatgaa agaacaagca
    54001 gaagcctatc aaaagggaga aaatatcctg acttatggac tgaaagaatg gtatccacaa
    54061 attcgaccaa tagtgggcaa atttttccag attgaacaag acttgactag ctattatcag
    54121 cacttttata cctattacca aaaaaatcct caaaatgatt ggcaaaagct ttatccacca
    54181 gccttttatc agcaatattt cttgaaaaat atggtagaat agaaaggatg gaggaatcta
    54241 atggtattac aaagaaatga aataaatgaa aaagatacat gggatctatc aacgatctac
    54301 ccaactgacc aggcttggga agaagcctta aaagatttaa cagaacaatt ggagacagta
    54361 gcccagtatg aaggccatct cttggatagt gcggataacc tactagaaat cactgaattt
    54421 tctcttgaaa tggaacgcca gatagagaag ctttacgttt atgctcatat gaagaatgac
    54481 caggatacac gtgaagctaa gtatcaagag tactatgcca aggccatgac actctacagc
    54541 cagttagacc aagccttttc attctatgag cctgaattta tggagattag cgaaaagcag
    54601 tatgctgact ttttagaagc tcaaccaaag ctgcaggttt atcaacacta ttttgacaag
    54661 ctcttgcaag gcaaggatca cgttctttca caacgtgaag aagaattatt ggctggagct
    54721 ggagaaatct ttggttcagc aagtgaaacc ttcgctatct tggacaatgc ggatattgtg
    54781 ttcccttatg tcctagacga tgatggtaaa gaagttcagc tatctcatgg gacttacaca
    54841 cgtttgatgg agtctaaaaa acgtgaggtt cgccgtggtg cctatcaagc tctttatgcg
    54901 acttacgaac aattccaaca cacctatgcc aaaaccttgc aaaccaatgt taaggtgcaa
    54961 aattaccgtg ctaaagttcg taactacaag agtgctcgtc atgcagccct cgcagcgaat
    55021 tttgttccag aaagtgttta tgacaatttg gtagcagcag ttcgcaagca tttgccactc
    55081 ttacatcgct atcttgagct tcgttcaaaa atcttgggga tttcagatct caagatgtac
    55141 gatgtctaca caccgctttc atctgttgaa tacagtttta cctaccaaga agccttgaaa
    55201 aaagcagaag atgctttggc agtcttgggt gaggattact tgagccgtgt taaacgtgcc
    55261 ttcagcgagc gttggattga tgtttacgaa aatcgaggca agcgttcagg tgcctactct
    55321 ggtggttctt acgataccaa tgcctttatg cttctcaact ggcaggacaa tctggacaat
    55381 ctctttactc ttgttcatga aacaggtcac agtatgcatt caagctatac tcgtgaaact
    55441 cagccttatg tttacggaga ttactctatc tttttggctg agattgcctc aactaccaat
    55501 gaaaatatct tgacggagaa attattggaa gaagtggaag acgacgcaac acgctttgct
    55561 attctcaata acttcctaga tggtttccgt ggaacaattt tccgccaaac tcaatttgct
    55621 gagtttgaac acgccattca ccaagcagat caaaatgggg aggtcttgac aagcgatttc
    55681 ctaaataaac tctacgcaga cttgaaccaa gagtattatg gtttgagtaa ggaagacaat
    55741 tctgaaatcc aatacgagtg ggctcgcatt ccacacttct actataacta ctatgtatat
    55801 caatattcaa ctggctttgc ggccgcctca gccttggctg aaaaaattgt ccatggtagt
    55861 caagaagacc gtgaccgcta tatcgactac ctcaaggcag gtaagtcgga ctatccactt
    55921 aatgtcatga gaaaagctgg tgttgatatg gagaaggaag actacctcaa cgatgccttt
    55981 gcagtctttg aacgccgttt aaatgagttt gaagcccttg ttgaaaaatt aggattggca
    56041 taaaatggtt gaatcgtata gtaagaatgc taaccataac atgcgtcgtc ctgtcgtcaa
    56101 agaagaaatt gtagacttga tgcgtcagcg tcaaaagcag gtcacaggtt tcttgaaaga
    56161 attggaagac tttgcccgca aggaaaatat tcctattatt ccccatgaaa cggttgctta
    56221 tttccgtttt attatggaaa ccatgcagcc taaaaatatt ctggaaattg ggacggctat
    56281 cggtttttca gctctcttga tggctgaaca tgcgccaaat gctaagatta caactattga
    56341 tcgtaatcca gaaatgattg gttttgccaa ggaaaatttt gcccagtttg acagtcgcaa
    56401 gcaaatcact ctcctagagg gagatgcggt ggatgtctta tctacactga cagagtctta
    56461 tgatttcgta tttatggatt ctgccaagtc taaatacatc gtctttctgc cagaaatcct
    56521 caaacatttg gaagttggtg gtgtggttgt cttggatgat atttttcaag gtggtgatgt
    56581 tgccaaggat attatggaag tccgtcgtgg tcagcgaacc atttatcgag gccttcaaaa
    56641 attatttgat gcaaccttag acaatccaga actcaccgca acattagtgc ctttaggaga
    56701 tggtattctc atgcttcgta aaaatgtagc agatgttcaa ctgtctgaaa gcgaatgatt
    56761 ttcagaaaaa tttaagaaaa aatagtaaaa tagatagagt aacacttatc tcaaaggagt
    56821 agacatgaag aaaaaattat tggcaggtgc catcacacta ttatcagtag caactttagc
    56881 agcttgttcg aaagggtcag aaggagcaga ccttatcagc atgaaagggg atgtcatcac
    56941 agaacatcaa ttttatgagc aagtgaaaaa caacccttca gcccaacaag tcttgttaaa
    57001 tatgaccatc caaaaagttt ttgaaaaaca atatggctca gagcttgatg ataaagaggt
    57061 tgatgatact attgccgaag aaaaaaaaca atatggcgaa aactaccaac gtgtcttgtc
    57121 acaagcaggt atgactcttg aaacacgtaa agctcaaatt cgtacaagta aattagttga
    57181 gttggcagtt aagaaggtag cagaagctga attgacagat gaagcctata agaaagcctt
    57241 tgatgagtac actccagatg taacggctca aatcatccgt cttaataatg aagataaggc
    57301 caaagaagtt ctcgaaaaag ccaaggcaga aggtgctgat tttgctcaat tagccaaaga
    57361 taattcaact gatgaaaaaa caaaagaaaa tggtggagaa attacctttg attctgcttc
    57421 aacagaagta cctgagcaag tcaaaaaagc cgctttcgct ttagatgtgg atggtgtttc
    57481 tgatgtgatt acagcaactg gcacacaagc ctacagtagc caatattaca ttgtaaaact
    57541 cactaagaaa acagaaaaat catctaatat tgatgactac aaagaaaaat taaaaactgt
    57601 tatcttgact caaaaacaaa atgattcaac atttgttcaa agcattatcg gaaaagaatt
    57661 gcaagcagcc aatatcaagg ttaaggacca agccttccaa aatatcttta cccaatatat
    57721 cggtggtgga gattcaagct caagcagtag tacatcaaac gaatagtcca aatcaatgag
    57781 tcagggaaaa aactcgactt caggaaaaaa tgaagcaaac attcccacaa taaaacgcat
    57841 agtacaaggt ttgtactgcc ccccaaaaaa gttagacaat taatttatcc gaaggattta
    57901 gttctgtatt gcacagagct aagtcctttt agttttatct taattctctt attgttgtaa
    57961 taatcaatat agtctataat ggcttgttcc aattgattaa gtgatttaaa tgtttgctca
    58021 tagccataaa acatttcgga ttttaaaatg ccaaagaaag attccatcct accgttgtct
    58081 tggctgtttc ctttgcgtga catagatgct tgaattccct tactctctag gaagcgatga
    58141 taagaatcgt gttggtattg ccagccttgg tcactatgga gaatcgtatt ctcgtagtgc
    58201 ttctctttga atgcctgttc caacattaac gatcaatcaa tttaatcat
//
LOCUS       DAVLYV010000006        48254 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLYV010000006 DAVLYV010000000
VERSION     DAVLYV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.006"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(306..1868)
                     /gene="guaA"
                     /locus_tag="VYO70_000344"
     CDS             complement(306..1868)
                     /gene="guaA"
                     /locus_tag="VYO70_000344"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1712635.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            2010..2708
                     /locus_tag="VYO70_000345"
     CDS             2010..2708
                     /locus_tag="VYO70_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1712636.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQISYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(2751..3647)
                     /locus_tag="VYO70_000346"
     CDS             complement(2751..3647)
                     /locus_tag="VYO70_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1712637.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(3656..4591)
                     /locus_tag="VYO70_000347"
     CDS             complement(3656..4591)
                     /locus_tag="VYO70_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1712638.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(4588..5313)
                     /locus_tag="VYO70_000348"
     CDS             complement(4588..5313)
                     /locus_tag="VYO70_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1712639.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(5397..6032)
                     /locus_tag="VYO70_000349"
     CDS             complement(5397..6032)
                     /locus_tag="VYO70_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1712640.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEREEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(6034..6861)
                     /locus_tag="VYO70_000350"
     CDS             complement(6034..6861)
                     /locus_tag="VYO70_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1712641.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALTHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(6883..7809)
                     /locus_tag="VYO70_000351"
                     /pseudo
     CDS             complement(6883..7809)
                     /locus_tag="VYO70_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809127.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
     gene            8460..9071
                     /locus_tag="VYO70_000352"
     CDS             8460..9071
                     /locus_tag="VYO70_000352"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1712642.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            9116..9844
                     /gene="rlmB"
                     /locus_tag="VYO70_000353"
     CDS             9116..9844
                     /gene="rlmB"
                     /locus_tag="VYO70_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1712643.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(9883..10794)
                     /gene="trxB"
                     /locus_tag="VYO70_000354"
     CDS             complement(9883..10794)
                     /gene="trxB"
                     /locus_tag="VYO70_000354"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1712644.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLIVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(10860..11084)
                     /locus_tag="VYO70_000355"
     CDS             complement(10860..11084)
                     /locus_tag="VYO70_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1712645.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(11162..11905)
                     /locus_tag="VYO70_000356"
     CDS             complement(11162..11905)
                     /locus_tag="VYO70_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1712646.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(11905..12705)
                     /locus_tag="VYO70_000357"
     CDS             complement(11905..12705)
                     /locus_tag="VYO70_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1712647.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(12863..13219)
                     /locus_tag="VYO70_000358"
     CDS             complement(12863..13219)
                     /locus_tag="VYO70_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1712648.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(13232..13744)
                     /locus_tag="VYO70_000359"
     CDS             complement(13232..13744)
                     /locus_tag="VYO70_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1712649.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(13744..14238)
                     /locus_tag="VYO70_000360"
     CDS             complement(13744..14238)
                     /locus_tag="VYO70_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1712650.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            14373..14819
                     /locus_tag="VYO70_000361"
     CDS             14373..14819
                     /locus_tag="VYO70_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1712651.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            14816..15463
                     /locus_tag="VYO70_000362"
     CDS             14816..15463
                     /locus_tag="VYO70_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1712652.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLVIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(15484..16065)
                     /gene="pdxT"
                     /locus_tag="VYO70_000363"
     CDS             complement(15484..16065)
                     /gene="pdxT"
                     /locus_tag="VYO70_000363"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1712653.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(16066..16941)
                     /gene="pdxS"
                     /locus_tag="VYO70_000364"
     CDS             complement(16066..16941)
                     /gene="pdxS"
                     /locus_tag="VYO70_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1712654.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(17093..18472)
                     /locus_tag="VYO70_000365"
     CDS             complement(17093..18472)
                     /locus_tag="VYO70_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1712655.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            18766..19689
                     /locus_tag="VYO70_000366"
     CDS             18766..19689
                     /locus_tag="VYO70_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1712656.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            19749..20354
                     /locus_tag="VYO70_000367"
     CDS             19749..20354
                     /locus_tag="VYO70_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1712657.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            20372..21616
                     /locus_tag="VYO70_000368"
     CDS             20372..21616
                     /locus_tag="VYO70_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1712658.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(21712..21969)
                     /locus_tag="VYO70_000369"
     CDS             complement(21712..21969)
                     /locus_tag="VYO70_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1712659.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(22011..24047)
                     /locus_tag="VYO70_000370"
     CDS             complement(22011..24047)
                     /locus_tag="VYO70_000370"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1712660.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQSVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(24259..25176)
                     /gene="glyQ"
                     /locus_tag="VYO70_000371"
     CDS             complement(24259..25176)
                     /gene="glyQ"
                     /locus_tag="VYO70_000371"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1712661.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(25544..26305)
                     /locus_tag="VYO70_000372"
     CDS             complement(25544..26305)
                     /locus_tag="VYO70_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1712662.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            complement(26578..27420)
                     /locus_tag="VYO70_000373"
     CDS             complement(26578..27420)
                     /locus_tag="VYO70_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1712663.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(27534..28925)
                     /locus_tag="VYO70_000374"
     CDS             complement(27534..28925)
                     /locus_tag="VYO70_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1712664.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKVEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDVSKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(29160..29537)
                     /locus_tag="VYO70_000375"
     CDS             complement(29160..29537)
                     /locus_tag="VYO70_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1712665.1"
                     /translation="MLCQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGTQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIAPV
                     YAIAIVDSNYFSDDLASHSFIVK"
     gene            29788..30765
                     /locus_tag="VYO70_000376"
     CDS             29788..30765
                     /locus_tag="VYO70_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1712666.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFADHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            30762..31844
                     /locus_tag="VYO70_000377"
     CDS             30762..31844
                     /locus_tag="VYO70_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1712667.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(31908..32943)
                     /locus_tag="VYO70_000378"
                     /pseudo
     CDS             complement(31908..32943)
                     /locus_tag="VYO70_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(33327..34523)
                     /gene="tuf"
                     /locus_tag="VYO70_000379"
     CDS             complement(33327..34523)
                     /gene="tuf"
                     /locus_tag="VYO70_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1712668.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(35445..36314)
                     /locus_tag="VYO70_000380"
     CDS             complement(35445..36314)
                     /locus_tag="VYO70_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1712669.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(36537..37058)
                     /locus_tag="VYO70_000381"
     CDS             complement(36537..37058)
                     /locus_tag="VYO70_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1712670.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKKDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            complement(37030..38121)
                     /locus_tag="VYO70_000382"
     CDS             complement(37030..38121)
                     /locus_tag="VYO70_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1712671.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSKGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKSGSQNSNLY"
     gene            complement(38232..38486)
                     /locus_tag="VYO70_000383"
     CDS             complement(38232..38486)
                     /locus_tag="VYO70_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712672.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(38844..39377)
                     /locus_tag="VYO70_000384"
     CDS             complement(38844..39377)
                     /locus_tag="VYO70_000384"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1712673.1"
                     /translation="MTSFKEQEPEKVAEFLDILDNLKDLPIVYIDETGIDRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(39364..39579)
                     /locus_tag="VYO70_000385"
     CDS             complement(39364..39579)
                     /locus_tag="VYO70_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1712674.1"
                     /translation="MGKERCEQEHIERKKRVVKNRKIPLEELKTFVEAHPDAFLREIA
                     AHFDCAVPSVWAALKQIKVTLKKDDEL"
     gene            complement(39546..39689)
                     /locus_tag="VYO70_000386"
     CDS             complement(39546..39689)
                     /locus_tag="VYO70_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tnpB"
                     /protein_id="HEV1712675.1"
                     /translation="MAYSTDFKQRALDYIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KNT"
     gene            39923..41641
                     /locus_tag="VYO70_000387"
     CDS             39923..41641
                     /locus_tag="VYO70_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1712676.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKITNIEAE
                     INAFVK"
     gene            41752..42099
                     /locus_tag="VYO70_000388"
     CDS             41752..42099
                     /locus_tag="VYO70_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1712677.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(42377..43213)
                     /locus_tag="VYO70_000389"
     CDS             complement(42377..43213)
                     /locus_tag="VYO70_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1712678.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTNLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(43226..43855)
                     /locus_tag="VYO70_000390"
     CDS             complement(43226..43855)
                     /locus_tag="VYO70_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1712679.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(43865..44506)
                     /locus_tag="VYO70_000391"
     CDS             complement(43865..44506)
                     /locus_tag="VYO70_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1712680.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(44652..45881)
                     /locus_tag="VYO70_000392"
     CDS             complement(44652..45881)
                     /locus_tag="VYO70_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1712681.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELANLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLAAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(45871..47037)
                     /locus_tag="VYO70_000393"
     CDS             complement(45871..47037)
                     /locus_tag="VYO70_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1712682.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            47181..48194
                     /locus_tag="VYO70_000394"
     CDS             47181..48194
                     /locus_tag="VYO70_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1712683.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
BASE COUNT        14322 a        10647 c         8687 g        14598 t
ORIGIN      
        1 attaaaatat cctccaatag ttttacttta ctaatagtat acaggaggaa ctccatgaat
       61 gatagatacc ttgttatgac gcgcttacta tattagaaag ttggctctct aattaaatta
      121 attctcatta tatatatcaa aagaccgcag tttgaaaact acggtctaat tttttgtcca
      181 aatgtaaatc acttggaact attattcaat ttattctttt tagtaaatgt tcccgtttta
      241 atcccagtta cagtttttaa tcctctgtaa ccgttgattt tacaaagctt ttaatttttt
      301 tgcgattact cccactcaac tgttgcaggt ggtttacttg taatatcgta gacgatacgg
      361 ttaacatgat ccacttcatt tacgatacgc actgagattt tttgaagtac ttcccatgga
      421 attttggcaa aatcagcagt cataccatcg atagaagtga tagcacggat tgcaatcgtg
      481 tagtcatacg tacgaccgtc acccataaca ccgactgaac gaacgcctgt gttaacagtg
      541 aagtattgcc aaatatcgcg gtcaagtcca gctttagcga tttcttcacg aagaatagcg
      601 tctgattcac gaacggtttc aagtttctct tcagtgattt cacccatgac acggatagca
      661 agtcctggtc ctgggaatgg ttggcgccat acgatatggt ctggcatacc aagctctgta
      721 ccaagagcac gaacttcatc cttgtaaaga gtattgagtg gttcaatcaa ttcaaactgc
      781 atatcttctg gaagaccacc cacgttgtgg tgtgacttga tagtttgagc tgtatccgta
      841 ccagactcga taacatctgt atataaagta ccttgagcaa ggaatttcac atctttgagc
      901 ttgcttgctt cgtcatcgaa tacatagaca aactcgttac caatgatttt acgtttttgt
      961 tcaggatcag aaacgccagc aagtttgtca aggaaacgtt tagcagcgtc tgctttgacg
     1021 atattcaaac caaacttacc accgagcatg tccataactt gatcagcttc gcctttacga
     1081 agaagaccgt ggtctacgaa gatacagatc aattgatcgc caatcgcttt ttggagaaga
     1141 accccaacga cagatgagtc aacaccacct gatagaccaa gaaggacacg tttatcaccg
     1201 acggtttcac gaattttttt gatctgcatg tcaatgaaat tatccattga ccagtcacct
     1261 ttagccttac aaatgttaag ggcaaagtta cgaaggatat catttccgta tacagaatga
     1321 cgaacttctg ggtggaattg gataccgtaa atgtgtttat ctgggttttc gatggctgcg
     1381 tatgggcagt cagctgatgt acctgtacga acaaagtcag caggaatctc agtaaccgca
     1441 tcaccatggc tcatcaaaac agtctgttca tcaggtgttg attcaaaaag cgctgatggt
     1501 gtgtgagtta gggttgattg accgtattca cgatttccag catcacctgc aggaacaact
     1561 tttcctccaa gtttatgggt caataactgc ataccataac agattcccaa aattggaatt
     1621 ccgagttcga agatttctgg gtcaatatca aatgaaccat cttcatatac agaatttgga
     1681 ccacctgata gaataattcc tacaggattg acttcacgaa cttcagcagc tgaaatttta
     1741 tggcttttta gttctgaaaa aacaccaatc tcacggatac ggcgtgaaat cagctggttg
     1801 tactggctac catagtccaa tacgatgatt ttttctacat cttgcaaatc agttgaaatg
     1861 ttgctcatct ttttcctttc tcaaaagaaa tatcttttct caatattcta tcacaaataa
     1921 gggcgaatta ccaactaaac aaggcgaagt ttgacttttt ctagtttatt ggggtacaat
     1981 agagaaaaga tagaaatgaa gtgaaaaata tgctacctgc ttatatgaaa atccatgatc
     2041 agataaaaaa ggatattgac gagcaccgtt gggctattgg tgagaggctt cccagtgaaa
     2101 gagatttagc tgagcagttt gcggtcagtc gcatgaccct ccgccaagcc gtatctctat
     2161 tagtcgaaga aggcgtctta gagcgccgtg taggaagcgg cacctttgtt tccagtactc
     2221 gagtacaaga aaagatgcga gggacaacca gttttactga aattgtcaaa tcccaaggta
     2281 aagttccctc tagccagctc atttcctaca aaaaaaccat tcccaatgag caagaagttg
     2341 ccaagctagg aatttttcca acggacaata ttatccgaat ggagcgggtc cgctatgccg
     2401 accaagttcc cctagtttat gaagttgctt ctattcctga aaaattcatt aaggacttta
     2461 aaaaagaaga aatcaccagt catttcttcc aaaccttgca aaaacatggc tatcgtatcg
     2521 gtaaatccca acagaccatc tatgctcgcc ttgctaaaga aaagattgcc cactatttgg
     2581 aagttgaaaa aggacatgct attcttggat tgacccagat ttcctaccta gaagatggta
     2641 ctgcttttga atacgtaaaa agtcaatatg taggcgaacg ctttgaattt tatcttgaaa
     2701 ataattagat taccacgcaa aaactctctg tcacggacaa agagtctttt ttatttttct
     2761 aagaataaat ctttcaaaga aatcaaagtt acagcaaaca atatcattgc cataccaaga
     2821 aaatcaatgg gataaaattg ttcattcatt attaagaagg caaagaaaat cgccgatatt
     2881 ggctcaattg aagccaacaa gcttgacttg accggtccta tcagactggc tcctttaagg
     2941 aaagctgtat aggcaaagac agtcccgata aggataatgc ctgcaaacgc aaggagaaaa
     3001 tcaagactag tcgggatatc ggcctgtaga acccctgtaa aaggaagggc gaccaaacct
     3061 gctatgacca ttcccacacc aatgaccaag ctgctccccc actttttaat caaggctatg
     3121 ggtaaaatga tatacagagc ataagtcaag gcagaaaaga gaccccagaa cagaccagca
     3181 ggtgtcatgg ataactggtc caactgccca tgtgttgcga tcaggaaggt tcctccgatg
     3241 gcgaatatga tggaaactat ctctcccagt gtcggtgcca ccctatcctt gatacagcta
     3301 taaattaaaa ttccgacagg acaaacatac tgaagcaccg tcgctgttcc cgcattggtc
     3361 tcctgaatag cagacagata ggcgaattgg ttgagaaaaa gaccaatcag agcaaaaata
     3421 agaagagaca gcaaactctt tctatccttt aaaaagacca gtattttatc ctttgcagta
     3481 gcataagcca agagcatgag aattccacca gcgattaaaa gacgcaagtt agtcaagacc
     3541 agagccgaaa ttccgtgtgc cattaggtat tggccactcg ttcctgacaa cccccaagca
     3601 ataccagcca caactgttag taaagtccct tttaaactat ttgacatgtc tctccttact
     3661 cttctttgcg aatcaagttc atcacttgat tgcggtctaa tatccattga gctctctcgt
     3721 ccttttcata ggtacgattc gataggaaaa tagtggcttc ttgcttctga cgattccaca
     3781 tgataaaggt acctgtatag cccgtatggt ctagccaatc tccttccaaa ttccatgcta
     3841 aagaacgttc cttgtcatcc aaaggagaaa aattttgatt taagtctctt gcaaaatcat
     3901 ctgctaaata gtgttctaaa aagatttgta aatcctttat agtcgaaaat aaaccagcac
     3961 tcccagcatg tctacccagg agacgagcct tgggatcatg cactatgcct gcctctacac
     4021 ctctaactgt tggaacagca agctcaactg gcccaaactt ggtttccgtc attccccaag
     4081 gtttccagac ttgatccttt aaaatcacat ccaaatcttg attaaaaatt ctttccaaaa
     4141 taaagcccaa cagcaaaaaa tggacatccg aataaagaaa ggctggctga cttcgtctgt
     4201 tgagatgaaa catcgcttcc tttaattcag gggctgttaa aagatcacga ttaggaataa
     4261 aaggatcaag gtctgttgca tgagtcaaga gctggcgaat agtgatgtct ggataatcac
     4321 tctcaggtaa aaaatctatt accagtctat caatatctaa ttgacctatt tcccacaaga
     4381 aggtacaaac tgtgccaacc ccaacaacct tgctgacact agctaggtca taaactagtc
     4441 ctgcctcagt ctgcaagcca tgctctgggt cactctggcc taaatagaac tctgtccatt
     4501 gattgtcctt aaaatacgca aaagaggctc cgggataaat ccctgcctcg atttgttctt
     4561 ctattttttt aataatcttg gtccacttca tacttcttca aaccacaatt caacattatt
     4621 tctatcttta ccaaggaaga atttttcaga cttaggaata aaatattcag tagactcaaa
     4681 cttctggcga agacttgcta tgtctaattc attgaccaag aacttgagca tagataagtc
     4741 ccaggtaacc gtattgtcca cagtcaaatc ctgcccctga gctggaataa aatcaaggga
     4801 tgccccaatt tcagatgatt ccaagaaact ttcgatatca gttgggagat gtaattccat
     4861 agaaatctca aatttactta aagaaattga tgccaaatct gtttgaaatt caggcttttc
     4921 tcctacttct actagacttg ctatgtcatc ttccgcatga atcaaaatca aatcatcttc
     4981 tggtgagaaa atttcaaaag cgtagccatt ttgaccttta tataatcgat gaatcgaatc
     5041 tgttttagat aagattcctt caatttctaa gggattttcc accttgacaa tcaatctagc
     5101 tagttttttt cttccctcta ccttacgagt acgcatactg ggagcttctt ctaaaaccag
     5161 cttttcaaga cccgtttggt cacctagtga cagaaaggcc gattcttcta acaaggcctt
     5221 cattccaagg gtttcaatat aaaatgtttc atttaatttt ctattattag cttttaaagt
     5281 aggaataatc cgtacaatct gatttacatt cataaattcc tccgtcactt tttattttaa
     5341 aggattttag aattttttac aagatattat gctcaaattt aacattattt agctatttag
     5401 taaagatagt ctttcaagga ttttgacaaa gcctgataac cagcaatact gaggtgcagt
     5461 ccatcagttg tatattcttt tttgagttgg cctgcttggt ctgtcaaaca atcaaatact
     5521 ggcacaaatt ccacctgcat ataggcagat gcaagctctt gataggcttg attccagttc
     5581 tgaatttttt cattcgagcg gatatagact gcctgctggt actcctctct ctcattgaca
     5641 ggcaaaatgg aaagcaattt aatctctgtc aatggataat cgcgagcaac ggattgaatg
     5701 atagcttcga gattattgag agcctcattc acaggaacat cctttccgat atcatttgtc
     5761 ccaatcagaa gaaaaatttt atctactgct ccaccatata gatgagcatc aaggttctct
     5821 agtaacagtc ctgtctgata gccacgaatt cctcgattga caatcgtctt tgaagtccca
     5881 aatagctcct gtagaggata atactcgaca atggaatccc caataaaaag aatgtttggt
     5941 tctacaacag aaatgtgatt taggtgacga tacttagttt gaattttttc ttgttccttt
     6001 aggagccaat tttctaataa ctgtactgcc acttcattct cctttttcta accagttttc
     6061 caagacttga taaactcctc cttggctgtt ggctggtgct agagcagtcg ccacagcttt
     6121 ggctttctca tcagcatttt ccatcgcata ggcaattcca gccatttcaa gcatttcaac
     6181 atcattttca ctatcaccaa aagccatgat ttcttgggat ttcaagtccc agcgcttgag
     6241 taattcctcc aagccccatg ctttatgaat cccagcttgg aggatatcaa tgcaaccata
     6301 gccactggat acagctcgga cacggccatc aaagagagca ttgatttctt ccaaaaccga
     6361 actcaaacgt tcctcaccaa caaccatgct catcttgagc acaccaccaa agaggtcaga
     6421 tgttaattca tccacaaatt gcatccgttg gtagaatttt tcaatcattt ctggagtcat
     6481 aaaactttca agatctgtaa aaatcgtacc ttccttgaca aaatcaccct tcatccccgt
     6541 tacaacaaac tggtcctgac acgctcgacc cttgaaatga gtcaaagcct tgttgacaat
     6601 ggcgtcatcc catgtctgag cctgaatcaa ttcattgttt tcaaaaatac gagcgccatt
     6661 agcaacaacc agaaccactc gatccaccaa gggactcagt agttgtctca tgcggtgaat
     6721 ttcattgccc gtcgcaatga caaaacgaat gcccctttga tccaactgat ctaaaatctt
     6781 ttccaatcgt gggagatcaa gctgacctct agcatccagc aaggtcccat ccatatctgt
     6841 cgcaattacc ttaatcatca tttactttcc tctggattca agctaatacc acttcaatcc
     6901 cacatgttca ttcatttctt tataggctgt atcaattcgt tccttagtcg cttcatctaa
     6961 ctggtcacga tgactgccat cctctataaa atcttccaaa atataagttt ggtaatgata
     7021 caagggatat ccttctggtg aaaaggttcc ctttggtgtt cgattgaccc aagtaggatg
     7081 agctttagct gttccgatag ttgtttttcc atccttcttc ttgatggtga catccatgag
     7141 aacaccacgt tcagtccact tagcattctc ttcatctccc atagattcaa ttcgttgatt
     7201 ggaaatgaag ttccccattt aatagataat gagtttctta tctccatctt tttcaaccgt
     7261 ttcagatggt tcaacaacgt gaggatgccc tccaaagata atatccgctc ccaaatcgat
     7321 catcttgtga taaagagctt tttgttcttc agttggttcc aatcgatact caacacccat
     7381 ctgaagcatg ataatggtga tatctgcttc cttctctgcc cgttcaattt caacctttat
     7441 cttatcttcg tttaaatatg aaagataacg attatagtct tcctgagaaa tatactgctc
     7501 aattccattg aaaccatagg aataggctaa caatgcaacc ttgataccat tcacttcctt
     7561 aatgaccagc ggagcctgat cacgtggttc gtgcgtataa actccgattg gagtgattcc
     7621 agctttctca ataatatcgg ccgttgaaat aactccctca atttgcgaat ccaaaatatg
     7681 attatgagct aaatccagca catgataacc tgcctcctta atagcatcca taacttcaac
     7741 aggagcatta aagagaagat aacccgctaa ataatgatcc ttattaatgg ttccttcaaa
     7801 atcaccaata gctaaatctg ctgcttagtc cccttgcttg agccaaggag tcacatactc
     7861 aaaattttca tgaaagtcat aggtaccgtc ttctttttta gctgaaaaga aaagtccatc
     7921 gtggtagagc aaatccccgt gggccataat tctggcagtt ttttcctctt cctactcctg
     7981 agacttttgc ttagtcccct cttgagaaat agtatctcgt ttttgactag tcaagggatt
     8041 ccttggaagc tttcaaacaa caagaccaag cccattgata aaccaactgc tagcaagagt
     8101 atcgccacaa atcccttatt gctccacttg cgataactcc taaaaagttt accaagccct
     8161 tcataaaacg aaaagctaaa ccaccctgat ttcgattttg tcttctttgt atcttcgttc
     8221 tccctacttt cttatgcaag ccttttcttt ttattatatc acagataagt atttctttca
     8281 caattgaatt gaacttccca tctattttct ataaatccta aatgccataa tgctttcaat
     8341 tcttgtcatt ttgtgatatc atgtagagga aatgaactaa tccacagtgg cttattccaa
     8401 gtataccact tgggctttgg cagtagctaa ctgcactaaa cataatataa ggagagaaaa
     8461 tgtctgcaat agaacgtatt acaaaagctg ctcacttaat tgatatgaac gatattatcc
     8521 gtgaagggaa tcctactcta cgcacggttg ctgaggaagt cactttcccc ctatctgacc
     8581 aggaaatcat cctaggcgaa aagatgatgc aattccttaa acattcccaa gatcctgtca
     8641 tggctgaaaa aatgggactc cgcggtggtg ttggactggc tgctccccag ttagatatct
     8701 caaaacgcat tatcgctgtt ttggtaccta atattgttga agaaggcgaa actccacagg
     8761 aagcctacga tttggaagcc attatgtaca atccaaaaat cgtctctcac tctgttcaag
     8821 atgctgctct tggcgaagga gaaggttgcc tgtctgttga ccgtaacgtg cctggctatg
     8881 ttgttcgcca tgcccgcgtt actgttgact actttgacaa agatggagaa aaacaccgta
     8941 tcaaactcaa aggctacaac tccattgttg ttcagcatga aattgaccac attaacggta
     9001 tcatgtttta cgatcgcatc aatgaaaaag acccatttgc agttaaagat ggtttactga
     9061 ttcttgaata aagaaaatcc cgttgcaaga cggggttttg tgttataata gaggcatgaa
     9121 aacaaatgat attgtctatg gtgtccacgc cgttaccgaa gctctccttg caaatacagg
     9181 aaacaaactc tacctccaag aagatctccg aggtaagaat gttgagaaag tcaaggaact
     9241 agctacagaa aagaaggtgt ccatttcttg gacatcaaaa aaatctctct ctgagatgac
     9301 tgaaggtgct gttcatcaag gttttgttct acgagtgtct gaatttgcct atagcgagct
     9361 agattacatc cttgcaaaaa cacgccaaga agaaaatcca cttctattga ttctagatgg
     9421 tctaaccgat ccccataatc tgggttctat cttgcgaaca gccgatgcga ccaatgtttc
     9481 aggtgtcatc attcccaagc accgtgctgt cggagtaact cctgtcgttg ccaaaacagc
     9541 cacaggtgct attgaacacg ttccaattgc ccgagtgaca aacctcagtc aaaccttaga
     9601 taaactcaag gatgaaggct tctggacctt tggaacggat atgaacggta ctccttgcca
     9661 caagtggaat acaaaaggga aaatcgccct catcattgga aatgaaggaa aaggtatctc
     9721 tagcaacatc aaaaaacaag tcgatgagat gattaccatt ccgatgaatg gacatgttca
     9781 aagccttaat gccagtgttg ctgcggccat tctcatgtac gaagttttcc gaaatagact
     9841 ataaaaaagt ttccagtcat ctgattggaa acttttttat gattaactat gttctgtgat
     9901 aaacttgtag gcttcttgac cagcgatagc tccatctcca actgctgttg ttacttggcg
     9961 aaggtctttc aagcgaacat ctccaactgc aaagataccg tcgactgcag tcttcatgtg
    10021 gctatctgtc acaatccagc ctgcctgatc ttggatattc aattctttaa caaaatcgct
    10081 aagagggtcc aaaccaacat agataaagac accaccgaag gcttgttctg tcacttgacc
    10141 tgttttcaca ttttcaaata cgactgattc tactcggttt tcacccttga tttcccttac
    10201 tacagaatcc cagataaagc tgattttttc attcgcaaag gcgcgatctt gtaaaacctt
    10261 ttgggcacga agttggtcac gacggtgaac aatggtaaca gtcttagcaa aacgagtcaa
    10321 gaagagggct tcttcaacag ctgaatctcc accaccaact atcaataaat cttggtcacg
    10381 gaagaaagca ccatcacaca cagcacagta agaaacacca cgactgttca gttcttcttc
    10441 tccaggcact cccaaaggac ggtgtttaga accagttgct acgataactg tacgtgtttc
    10501 atatgtttgg tcatcagtca tcactttctt aaaatcacca tggtcttcga cattttcaac
    10561 ataaccataa atgtgctcaa caccaagatt ttcaagtggt tcaaacatct tttcagccaa
    10621 ttcaggtcca ctaatattag cgtatcctgg gtaattttcg atatcagatg tattattcat
    10681 ctgaccacct ggcagaccac cttcaatcaa agctactttt agattgcttc gagcagcata
    10741 caaggccgca gtcatccctg caggtccagc accgataata atagtatcgt acatatagat
    10801 tccttctttc ttgttgtaac tatctttatt ctaactcttt cttgtcaatc acgcaaggat
    10861 tatgccttga aaacaagaat taaactcata accgcaaagg ataatactgg gacaaccaag
    10921 actccaatca taatcatatc atagagatgg gcttggcgag ccttggctgt cttatcaact
    10981 cccgacatgg ctctcagacc aatccaaatc cctaaaaaaa tcagaacaag gatggtggtc
    11041 aatatcaaac tctcgaaata taaagaaaat agttgcagta gcatgatttc tctcatttct
    11101 atctttttta aagagtaaac tcagctagtc caactaactg agttttcctt tatctattat
    11161 atcaaatata agtccgtttg taactagcga agaattcttt tgtccgctct tctttagggg
    11221 tgtggataat ctcatccgga gttccagact cgatgatttt ccccttatct aagaagagaa
    11281 tcttatccgc aacttgggct acaaaggaca tgtcatgact gaccaaaatc atggtctgac
    11341 ctgacttagc agcatctgca atagactttt ctacttcacc gaccaattct gggtcaaggg
    11401 ctgaagttgg ttcgtctaag agcaaaacat ctggtttcat agcaagcgca cgcgctaggg
    11461 caacccgttg cttctgtcca cctgataaat ggcgaggata atggttttca cggtccgaaa
    11521 gcccaacctt agccaactct tccttggcaa tcttagtcgc ttcttggtca gataatttct
    11581 tgacaacaac caagccttct ttcacattat caagtgctgt tcggcgttca aacaaattaa
    11641 actgttggaa aaccatagac aacttacggc gtagggcaag gatttcttct tgagtgattt
    11701 tagaaaaatc aactgaaaaa ccatcaatct gaatagagcc actgtcaggt gtttcaagat
    11761 aattgagact gcgaagaaag gttgattttc cagctcctga agaaccaatc aaggctacaa
    11821 cttccccttt ttgaatatcc aagtccagat gatccaagac agtctgtcct gaaaaggatt
    11881 tgcttaaatt cgaaatctta atcattaacg aaggtctcct ttcacatctg tttgcactgt
    11941 atcaggtgca gaaatagcca tttttctctc gatgaaacga ccgaggcttt caattccgat
    12001 attgactacc caataaacaa gggcaacgga gatgaagcgt tcaaaatagc gataatcagc
    12061 tccacctaga atctgagctt gggcaaagac ttccacaaca cccgcactaa aagctagaga
    12121 tgttcccttg gtcaaaccga tgagggaatt aatcaaggtt ggagtagcta ccaccgctgc
    12181 attaggaata atcactcgtc gataaacttg cgctcgggtc atacccagac tgcgtgccgc
    12241 ctcaatctca ccaggattaa ctgagagaat ggctgcacga atggtttcac tagcataagc
    12301 tgcctcatta aaggcaaaag cgacaatcgc aaaagctgca gctggaatcg cattgatatt
    12361 gagaccagtt ccccattgct gattgagggc tttcaaagcc aaagggattc cgtagtaggt
    12421 caacatgagt tgcaccaaaa tcggtgtccc ttttaagaaa ctaacaaaga aggcctgcaa
    12481 gggatataaa atcttgacac gattgatctt cacaatggca aaaagaagcg ccaaaaccaa
    12541 gccaaaaagg gcaccaccaa ttgtcaacat aattgttgtt ggaagttgtt ggacaattct
    12601 agggattcca tcaaagaccg aacgtaggct aaacagcttg ccatccggaa tcaattgcat
    12661 caagttttgg taccaatctg atgctaaaat cgttgtaaca ttcataaaaa catcctcttc
    12721 tgataatgat tcattctacc atgcttctgc cgtcaatgaa attatttgct acggtcagat
    12781 acagactttg tctacctact agtttatcat actttgaaac agggaagtta tatattttat
    12841 ctatcaaaga gatagataaa aactatttca gtcccagttc ttcataagat tttcgataac
    12901 cgatttgctt aacagttcca ttttctacta aaatgggccg ttttaacaac ataccgtcac
    12961 ttgctagcaa ctcagccgct tcttggtttg acaaacttcc taccttatct tttagcccta
    13021 attcacggta tttgatacca ctggtgttga aaaattgctt caattcaaat cctgaggttt
    13081 ctagccaatt caaaatgact tcttggctag gtgtttcttc cacgatatgg acggctttat
    13141 agtccacacc taattgattt aattcttgtt ttgctttttt acaagttgaa catttggggt
    13201 attcgataaa ttctaacatg tgcttctcct tctatttgtt tatatcttta aaatctacgc
    13261 cttcatgctc caagagccaa gctttctttt ccactcctgc agcatagcct gtcagacgct
    13321 tgcctgctcc caacacacga tgacaaggta ctaggataga ccaaggattg cgtcccactg
    13381 ctccaccaat tgcttgagca gaagccactt gcaggtcttg agcaatttgt ccataggtca
    13441 ctgtctgacc ataaggaatg ccctgtaaat agtcccaaac tctcttttca aaattcgttc
    13501 cgattggcgc caagagcaag ttggataaat cctgaggctt gcctttaaag taatcatcta
    13561 agcaagcaat aactgggtct aaaataggat gactaacaac ttcttctatc gtttcatctc
    13621 ctagtcccct ctcaaaatgc ttctgctcct gaacccaaat tccatacaaa taatggtcat
    13681 cagctacaag tgatagaata ccaattggtg aggagtagag tatttttaca tacttctttt
    13741 gcattatttc tttaattttt gataggccaa gcgttcgcca tctactggca ccttaccaac
    13801 ttgtttaaaa ccaagttttt caaaaatatg ttgcataacc ttgttttcag catgcgtatc
    13861 tgagcgaaaa tcaagataat caaaaccttc aatcaagccc tctaagaagg tttgagcaac
    13921 tccttttccc tgcacatctg ctgccacagc aatacggtga aagactagat actctgactc
    13981 tccagcttgc cagtttccct cataaatagc ttcataggcc tcctctggac tcttggtcac
    14041 agcagcatag gctagcagtt ctccctcttc caaggctacg taggcttgcc ctgagatgat
    14101 atcatcgata ataacatctg catttggata gccattttgc cactggtcac taccagcatc
    14161 tgctaaacat tttttggcat cctccatcac ctgcatgatg gcatctactt catttggaaa
    14221 agctaaacga atttccatct tttttcctca tttctgacta gtttctccca tcatagcata
    14281 atttaagtat ttctacaagc agttaaaact tgactttgtg tttttgttat tttataatct
    14341 agttatcaaa actatataaa aaggagttga aaatgaaaac taccttttcc tacccaaaat
    14401 gggcagaaat tccaaacatt gacctctatc tggaccaggt tttgctctat gtcaatcagg
    14461 tctgcgcccc tatctctcct aataaagaca agggcctaac agcatctatg gtcaacaatt
    14521 atgtgaaaaa tggttacctg acaaagcctg acaaaaaaaa ataccaacgc caacagattg
    14581 cccgtttgat tgctatcaca accctcaagt ctgtattttc tattcaagaa atagcccaga
    14641 cacttaacac tctacaaact caagcaagtt cagaccaact ctacgatgct tttgtggact
    14701 atatgaacca aggaattgac ccagctaacc ctattatcca aaccagctgc caaaccgtta
    14761 aactctatca tcaaactcta gacttaatcg atcatactca agaggaggta atccaatgaa
    14821 cactagtctt aaactcagca aacaactcag ttttggagag gagattgcta atagcgtgac
    14881 ccatgctgtg ggtgcagtca tcatgcttat cttgctgcct atttcatcca tctatagtta
    14941 tgaagcacac ggatttttat cctctatcgg cgtttccatt ttcgtcatca gtctctttct
    15001 catgttccta tcatccacca tttatcactc tatggcctat ggttcgaccc acaaatatgt
    15061 tttgcgaatc attgaccatt ctatgattta cgttgccatt gccggctcat acacgcccgt
    15121 tgtcttgacc ttgatgaata actggtttgg ctatctgatt attgtcatcc aatggggaac
    15181 gaccatcttt ggtattctct ataaaatctt tgctaaaaag gtcaatgaga aatttagcct
    15241 tgctctttac ctgattatgg gctggttggt tctggttatc attcctgcca ttatcagtca
    15301 aacgacaccc gttttctgga gtctcatggt aactggcgga ctctgttata cagttggagc
    15361 tggattttat gccaagaaaa aaccttattt ccacatgatt tggcatctct ttatcctagc
    15421 tgcgtccgca cttcaataca tcgctattgt ttattacatg taaaaaagtt gagaaattca
    15481 atctcaactt ttttctttac acatattgat aaagtactgg tgcaagcgca catcatcagt
    15541 caattctgga tgaaaagaac ttaccaacat atttttttct tgggctgcaa caatttgatt
    15601 gttcactgtt gctaaaattt ctacaccctc accaacacta ctgataatcg gaccacggat
    15661 aaaggtcatt ggaatcttgc caactccctt acattctgct tccgtgtaga aacttcctaa
    15721 ttggcgccca taagcattac gctcgaccac catatccata gttcctagat gactctcttt
    15781 ctgagaagtg atttccttag ccagcaaaat taagcccgca caggtcccaa acactggtaa
    15841 gccagataga atggcttctc ggatgggaag tagcatgttc tggtcacgta agagcttgcc
    15901 catggttgta gactcaccac caggcaaaat caaacccgac aagtcactct gatcttgctg
    15961 aaaatcatct agatttctga gttctacact ctcgacacct aattgatcta gcacttttgc
    16021 atgttctgca aaggcccctt gcaaggccaa tattccgatt ttcatctatt ttcctcgttc
    16081 agccatgaga atttggattt cattttcatt aataccaacc atggcttctc ctaaatcttc
    16141 agagatttga gctaggattt gaggattacg gaagttagtc acagccttaa caatggcact
    16201 cgctcgttta acaggatctc ctgacttgaa aatacctgaa ccgacaaaga ccccctctgc
    16261 ccctaattgc atcattaacg cagcatctgc tggcgttgca acacctccag cagcgaaatt
    16321 tacaactggc aattttccat gttcatgaac atattggacc aattctacag ggacttgcaa
    16381 atccttggca gcaacataaa gctcgtcctc acgtaagttt tgaatgcggc gaatttcctg
    16441 attcatcata cgcatatgac gaacagcttg gacgatatcc cctgtccctg gttctccttt
    16501 ggtacgaatc atggaagcac cttcagcgat acgacgcaag gcttcaccca aatccttagc
    16561 accacagaca aaaggaactt ggaattcttt cttgtccaca tggaaacggt catcagctgg
    16621 agatagaact tcactctcgt cgatataatc aatttcaata gcctctaaaa tctgagcttc
    16681 aacaaaatgc ccgattctga ccttagccat tactggaata ctaaccgctt cttggatttc
    16741 cttaatcatc tttgggtcgc tcatgcggga aactcctcca gctgcacgaa tatcagccgg
    16801 aattcgttcc aaggccatca cagctgccgc accagcagct tctgcgatac gagcctgttc
    16861 aggattctgc acatccataa taacaccacc cttgagcatc tgtgccaagt ttttatttag
    16921 ttcataacga ttttcagtca tttgttttat cctcatcatt tgtattggta gctacctttt
    16981 cattttaagt cagaacagaa tgaagcacaa gataaaaaaa ctataattgt atggggacag
    17041 attggctaaa aattatctga ccttaactta aggccagata gctcattcat ttctattttt
    17101 cagccgtaag ggcagccatt gtgatgtagt tgtatggttt gttgaagtgt ggcaagaaga
    17161 agaggtctgt caatgccaat ttatcaattg tcacatgctc ttggatagca agtgagaaca
    17221 tgtggattcc catgctaatt gcaatatcat gtgaaaccat ttgggcacca agaatttcac
    17281 ggctatcttt gtcaaagaca atcttaattg ctacttcatg gttgtcatgt ttcatgaatt
    17341 ctggtttttg aagatcgtta aagcctgttt cagttgcgtt gtaaccagca gctttcgctt
    17401 tttcaagagt caaaccagtt gaaaccatgt gaagaccgta gattgagata ccatttgaac
    17461 cttgaacacc gattccttcc aattcatgtc cacaagcatt gtaggcacca acgataccag
    17521 tgcgaacagc atttgaagca agagcgatat agcttgtatc tttacgagca ttgtcataaa
    17581 cagtcgcaca gtcaccaaca gcgtaaacgc ctgggataga tgtttcttgt ttcttgtcta
    17641 caaggaaggc accgttgcgg aagagttcga tcttaccatc tgcaagggct gtgtttggac
    17701 ggaaaccaac tgcaaggata accatatcca cgtcaaagct ttctttgtca gtaatcaagc
    17761 gttcaacttt accgtcacct tcgattgctt taacagtttg acctagagcc aagcggatgt
    17821 tgtgatcttc caagttcttc gccatcattt gtgtgaagtc tttgtcatag taaccgttca
    17881 agacagtatc aacgatatca acaaggacaa cttcttttcc aagacgttca aaggcttcag
    17941 caagttcaac accgatgtaa ccaccaccaa caacggcgat acggtcgagg tgttggctct
    18001 tgtcagaaag tttattgata acttcttcag cattttggta caatttcacg aattgtacgt
    18061 tttcaagagt tgctttaaat tcgcggtttc ctttaacaat ttcaacacct tcgattggtg
    18121 gcaagattgg tgtagaacct gtagcgaaaa tcaatttttc gtatgattct ttgtgctctt
    18181 ttccttcaac ttccgctgta actactttgt tatcatagtc gattgaaaga acaggtgagt
    18241 tcatgtaaac tttagcacct ttagcttcca atttttcttt atcagaatag aacaagcctt
    18301 cagcaccgtc aatttgttca ccaatccaaa gagccattcc acatcctagg aaagagatgt
    18361 tagagttttg gtcaaataca acaatttcgt tctcatttcc aaaattatcc aacatggtat
    18421 tgatacatgc tgtaccagcg tggttagcac cgactacaac gattttactc ataaaaattt
    18481 cctgccttca ttctaaattt accttactag tataccattt accgttagcg tttacaaggg
    18541 gggtactatt atttcagcat tttttatcaa aaataattcc ccatttatcc tgttttcaag
    18601 cctgatgaat tttatgagca tatttcttga ctttttccaa attttgtttg tgaaaattct
    18661 aactttatgg tattataata acatgaaaat gaaatgtaag ggcttcaatt tagatgatta
    18721 tttgatatta caacttattt tctataagag aaaggagttg gtagcttgac tcttcgttca
    18781 cattctgaac ggctaatggg gactactatc actatttcat tagtggatga gcaagccgat
    18841 atctttctcc aaaaatcctt tgacttgctc aaagaacttg aataccgctt caacgccaat
    18901 agtcaagaat ctgagttgat ggaaatcaat tatcaagcag gaatagcccc cgtcacggtt
    18961 catccagacc tgtttgagct gatttcactt gggttagagc atagcctagc gctctctagc
    19021 cacctcaata tcagcattgg tcccttaatt caaacctggc gtatcggttt ttcagatgcc
    19081 aaggtcgcgc agcctcaaga aattgaatcg gtgctgcctc taatcaatcc tcatggtatc
    19141 gagttagatt cttctacttc tactgtgttt ttaaaacaga aaggaatgaa gatcgatcta
    19201 ggttgtttag ccaaaggata cagtgcggat aaggttgccc aatttcttag gaaagagggg
    19261 gtgacttctg ccttgatcaa tctgggaggg aatatcctga ccattggaaa aaatcaggca
    19321 agaggggata acccatggca aatcgggatt caagacccag ccaatcctag gggaaatcat
    19381 ttaatgacca tccctgttgt caataaatct gtcgtgactt caggcattta tgaacgtcac
    19441 ctgaccgtcg atggacaaga ttaccatcac atttttgaca gtcaaacagg atatcctgtt
    19501 gaaacggaac tagcgagtct aacaatcatc tctgataaat cagtcgatgg cgaaatctgg
    19561 acgactcgac tctttggaga aagacctgct tctatcctct ggcaagtcga aagtttggag
    19621 ggcatcgaag tcatcctcat cgataaagaa ggccacctaa gctgttcttc aggaattcct
    19681 actctataga gacaactgtt tcaatggaat tttaaaattg tattatgtaa aaagagaaag
    19741 gaaatctcat gttaaaactt attgctattg ttggaacaaa ttcaaaacgt tctacaaacc
    19801 gtcaattgct tcaatacatg caaaaacact ttgctgacaa agctgaaatt gaacttgttg
    19861 aaatcaagac cattcctgtc ttcaacaaac cagctgacaa gcaagtacct gctgaaatat
    19921 tggaaattgc tgctaaaatc gaagaggcag atggcgttat tatcggtact cctgagtatg
    19981 atcactctat tccagctgtt ttgatgagcg ctcttgcttg gttgtcttat ggtatttacc
    20041 cacttttgaa caaaccaatc atgattacag gtgcttctta cggtacgctt ggttcatctc
    20101 gtgcccaatt gcaacttcgt caaatcttga atgctcctga aatcaaggca aatgttcttc
    20161 cagatgaatt cttgctctca cactctcttc aagcatttaa cccaagtggc gacttggttg
    20221 accttgatgt tatcaagaaa ttggatgcca tctttgatga cttccgtatc ttcgtgaaga
    20281 ttactgagaa attgcgcaat gcgcaagaat tgcttcgcaa agatgctgaa gactttgact
    20341 gggaaaattt gtaagatagg agaccgaaag aatgaaattt gttggacttg ttggatcaaa
    20401 ctacgatcaa tcatataacc gcaaactctt ggaatttatc cgtcgcaatt tcaaattcaa
    20461 atttgaatta gaagtacttg aaatcgacga agttccaatg tttaaccaag atgaaaaatg
    20521 ggacgaaagc ttccaattgc gtttcttgta taacaagatt acacgtgctg atggtgtcat
    20581 tatcgccact cctgagcaca accacactat ctcagcttca ctcaaatctg tacttgaatg
    20641 gctttcatac gaagttcatc catttgaaaa caagcctgtt atgattgtgg gtgcatcata
    20701 ctatgaccaa ggaacatcac gtgctcaagt tcaccttcgt aaaatccttg atgctccagg
    20761 tgttaatgcc tacacgcttc caggtaacga attccttctt ggtaaagcta aggaagcttt
    20821 tgataacaac ggaaatatca ccaacgaagg cactgttaaa ttccttgaaa cttgcttaga
    20881 taactttgtt aaatacgtag gagtcgtttc gaaattgaaa aaaccaaaac caatcgaatc
    20941 agaagacttg gattgtggaa aaccaatcgc tacaaccatc acagaagttg accctgacga
    21001 tccagaatgg gtagaaaaag ttgcagcaat cactggtgct gtttctggtg atacctatgt
    21061 caaattggac cacggtatcc taacagttaa ccaaattgat atgttcttga aagctatgcc
    21121 atttgaattg acatacgctg acgacaacaa ccaattcctc tactacaaca acgctcacca
    21181 agatccagac actatgttcg ctaaacgtgt accacctcaa tcaggtagcc gtatgtcgac
    21241 tgttcatggt tctcttccac cagcacgtat gaagaacgta gagtgggtta tcggaacact
    21301 tcgcaacgga aaccaagaat acgtccgtac gatcgttcca ggttctcctg caggtgttat
    21361 caacactcac aactaccaag caatgtacta tcctgatgga tcatacgctg gtatcaatga
    21421 aatcgtcttt aacttccaac catggcttga ctggtaccta aaagagactg gtcaacgttt
    21481 ggtgggtggt agcggaccat ttgctcctgc tgccggaggt catggagacg ccgatgctac
    21541 ttctggcgct tctgattcag gggatgctgg aggccacggt ggtgacgcag acgctacagc
    21601 cggtgcaagt tactaatgat agtttttagg aaccattatg gttccttttt tagcgaaaaa
    21661 agactagcaa acacaaactg ctagtctctt tgttaaaaga aagagtatat attatgaatt
    21721 tgggtcatca agactacggc catgtaatcc tttttcacgt tgtacttggc gtagtttttc
    21781 tggtgtaaca tcgtttcctt cttcgtccac aattttgatt ccttcaatgt ggtgacgaac
    21841 agcgcggcga taaccttcga tgtactcctc acgtagtttg gcttgttcca ctttttcttc
    21901 tggtgttaag ccttctgttt ttttcttttt agcaagctca ttgatacgag caattttttt
    21961 cggatccatt tcttctcctt tgtaataaga taaagtccgt ttatcaaatt ttatttagtg
    22021 ttaatttggt taaaacaagc aaacttagct gctttcttgg ttagttgtga caagattgcc
    22081 aaacgatttt gacggacagc ctgatcttca gccattacca tagtattttc aaagaaagca
    22141 tcaatgactg ggctaagcgc aaagagttgt ttcaattgct gacttgcagg tcctgataaa
    22201 acgagtgttt ctactgcttc tgccaaagct ttttcttggt cattctcaaa tagttctgaa
    22261 tcaactgtag caaccccttc tgccttctca gccaggttaa aggcacgaga aagtgattca
    22321 acagatggtt taaaatcttc ttccttgctt acttctacga gagcacttgc tgcttccaac
    22381 atatctgcca caacaaagtt tgaacctgca agaactgctt ccttgatatc ttttggagta
    22441 gagcccatca tcttatcaac acgagccttg ataaagtcca taacctctgc tttattttca
    22501 taagtcaaac tgtcaaattt caatgcataa aggctatcaa tcagctcatc catagcaatg
    22561 tgccaaccaa aggcatccaa gatacgaacc acaccttgag ttgcacgacg aagggcataa
    22621 gggtcattag aacctgatgg aatcaatcct actgagaaga aactcaaaat cgtatccaat
    22681 ttgtctgcaa tggctagaac tgcgccgacc ttgctctctg gaagttctcc ttcagctgat
    22741 gtaggcatgt agtgttcacg aatagcagct gccaccgctg gagtttcacc agcaagaagg
    22801 gtgtattttt cacccataat tccttggagt tcgtcaaatt caccaaccat acctgtcaac
    22861 aagtcaaact tgtaaatggc tgctgcacga gcaaggtcaa ctgtttcatc cactgacaaa
    22921 ccagcttttt ctgccaaaag tacagtgatt tgacccgtac gaatcatgtg ttcacgaagg
    22981 gaaccaatct tctcatggaa ggtgacattg tttaattttt caacaagatc tgaaatcacc
    23041 aatttttggt cttcacgcca gaagaattct ccgtcttcca agcgggctac caagactttt
    23101 tcatttcctt tgatgacatt tttcaaacgc tctgcgtttc cgttacgaac agaaatgaag
    23161 tttggcaaga gttttccatc ttgatcacga acaacaaagt aacgctggtg ttccttcata
    23221 gaagtcacca agacttcttc tggaacttca aggtatttag catcaaaact tcccatgaag
    23281 gcagttgggt attcaaccaa attcaagact tcattcagca aatccgcatc aatttcgata
    23341 cgtacaccat gttttgcctc aatttccttg atttggtcaa caatcatttg ctcacgttca
    23401 catggatctg cgattacaaa ctgcttacga aggtcttctt catagctcaa tgctgactga
    23461 attttggttt ctttccccaa gaaacgatgg ccacggctca cacgacttcc cttgatatca
    23521 aggaaatcca agtcaaactc ttgctcatcc aagagaacag ttaaagtgtg gacaggacga
    23581 atgtattcaa agctatttcc agcccagtgc atgctgacag ggaaagtcaa tgacttcaag
    23641 acatctacaa caccaggaac aatggcttca actgattggc caacttcttc cttagtgaca
    23701 tagacatatt cttcaccctt aatttcacgg aattcgatat cttcaacagt caaacctttc
    23761 ccacggacaa atccttgagc tgctttggtg aagtttccat cactatccaa ggcaattttc
    23821 tttgctggtc ccttgaaatc ttctgtcaaa tcagactgtt tgtctgataa accagtcaca
    23881 cgaacagcca aacgacgtgg tgttgagaag gtttgaatgg cttcaaaaga caggcggttt
    23941 tccttgagga aggctgccat tttttcgcct agttgttttt cacttggtgt gacaacatag
    24001 gctggtaatt cttcaagacc gagttctact aataagtttt ttgtcatgtt ttttctccga
    24061 aaagtatctt tttattttcc agtttgcctt atgtgattgg cacgcaagca accaacttcg
    24121 ttgtttcatt gctaaaattg gagccaaaag tctccaattt tgcctagtat ccttagtttc
    24181 actaaggata ccttcatcac tgcggcaact ggctcagggc tcactctgtt cgcccatttt
    24241 cgtaatttgt cactctcttt attctgcgtc ttctgctagg agtttagctc ttgtttcttc
    24301 atccaaaagt gggtagccta ggcgtttgcg ttctgcgaca aaggttttgg ctacgacacg
    24361 ggccaagtta cggatacgag cgatatagcc tgcacgctct gttacagata cggcaccacg
    24421 cgcgtcaagc agattaaagg tatgtgaaca tttgagaaca tagtcatagg cagggtgtac
    24481 caagccttct tctaatgcac gaccagcttc tttttcaaac ttatcaaagt tttcaagcaa
    24541 catttcttgg tccgaaattt caaatgaata ttttgagtgc tcatactcag gctggataaa
    24601 gatttctccg tattttacac catcagccca ctcgatatca tagacagagt ctacttcttg
    24661 aatgtaagaa gccaagcgct ccaaaccata ggtaacttcc gcagtcacag ggccagttgc
    24721 caatccaccg acttgttgga aataagtgaa ctgagtgatt tccattccgt caagccaaac
    24781 ttcccaacca agaccagctg aaccagttga tgggttttcc cagttgtcct caacaaaacg
    24841 aatatcgtgc tccaaaggat tgattcccaa tttttccaaa gactcaaggt aaagttcttg
    24901 gatatttgat ggagaaggct tcatgaccac ctggaattgg tggtgttggt agagacggtt
    24961 agggttttcc ccataacgac cgtcagcagg acgacgtgat ggctctacat aagctgcatt
    25021 ccatggctca ggtccgatag cacgaagaaa agtgtaagga ctcattgttc ccgcaccttt
    25081 ttcattatca taagcctgca taagcataca accttggtca ttccaaaatt gttgcaaagt
    25141 aaggatgatt tcctggaaag tcaatttctt agacattgtt tactcctttt tatataaatt
    25201 acttgcgaca tgaggctgcc tcgtcgatca tacgaggcaa gacgagttag tactgcgtct
    25261 agctcaggca ctctagtgct gagcgtgggg cagtccgact gtaaggaggt ttctgccact
    25321 gatgcagtga aatgttaatt tctcagacat tgaatactcc ttaataaaaa ataatttctt
    25381 gcgacacgag tctgcctcgt cgattatacg aggcaagacg agttagtact gcgtctagct
    25441 cagcacccta gtgctgagcg tggagcagtc cgactgtaag gaggtttctg ccactgacgc
    25501 agtgaaatgt caatttctta gacattgttt actcctttag tttttatatt tctttttcaa
    25561 ttaatttctg ctgaacaaac caatctaaca agggaaaagt tttttctgta tttgcccata
    25621 aatgataaga atctaagacc gcttgtacac tacctaaatt ttctaccaac ttatctactg
    25681 tcaaaaagct gcgccagtat tcataaaaaa tacttgcata gttcttatca taagtcgaag
    25741 aaccaaaatc attcaatgaa tgccaaccat acttcttctg aaaaagttct acgagagatt
    25801 gattacaaat tttttccgct tgaaattcct cttctgtcaa gaaatacttg cgactaatat
    25861 attcaaccat cccctcttcg aaccagatat aagagtcata accatcaaaa tcatctaaaa
    25921 aatgctccga ccaatgagct aactcatgtc ctacaatctg taagaggaaa ttttcagata
    25981 aagaatgata gtgactttct attgcttgag tttgctgaga acactcgtag tccatcaatt
    26041 gatacaaata caactctttc caaacagcta aatcaggcgc cataaccatt cgtctattat
    26101 ttgtataggc tggaacagcg ctttccctaa taatctgtgt agcagcatta aaatctgacc
    26161 aaataacggc ttgcggtaaa tcacagactg caagttcatt ctttaaaaaa gattgataat
    26221 ctttcaactt ttcagtattt cttactacaa aatcacgaaa tgtagctagt tgactatcgt
    26281 cttttacaag ataaaggttg tccacggctc tcctttcttt cacacaatag actgaataag
    26341 caaaagaacc gcgtccgaca atcctaggtt gatttaacct aggggcgtca gaaacgcggt
    26401 tccaccctaa tttattactt cattgttttt gaaaatacta cagaaagcgc cagctcatta
    26461 ttttgcccta cttggctccc actatcccaa gctcgcttga agaacgccat aagactttct
    26521 ttcctgctga ctattatatc aaaaattaga ataatgtaca attcttttta tgattttcta
    26581 gaaatccaca tcatcaactc gtggagcacc cgattgaaca gcaatcgttt ttaaggtttc
    26641 tctctcctca tgactcagtt caattccaaa gcaatcaaga ttagcttgaa tacgagaggt
    26701 tgtgacagat tttggaagtg gtaaaaatcc ttctgccaag ctccaggcca aggctatctg
    26761 agcaatcgat tttccgtgat ttgctgcgat ttcttgcact tgcttgctat caaatagttc
    26821 tccctgacca aaaggccccc aagcttccaa taaaattccc ttttcacgac agtaagctac
    26881 gacttgatct tgatacacac ctggcgccaa gcgaacttga ttgaccgcag gaacgatagt
    26941 tgcagtttca agcaaggcat ccaaatgatg gggaagaaaa ttgctaacgc cgatagcacg
    27001 gattttccct tcttgataga ggtcttccat cgctctccaa acttccgcat tgcgagtttt
    27061 ccatgcgtca ttttctctga gcggttttgg gttcggccaa tgaatcaaat acaaatccaa
    27121 ataatccaag cccagttttt ctatagattt ttccaaagct tgacgagttt gctcataggt
    27181 ttgctgacta ttccaaagct tggtagttac gaacatttct tcacgtggaa ctccgctatc
    27241 tttgattgct tgaccaacac tttcttcatt ctgataaatc gccgccgtat caatatgacg
    27301 ataaccagcc ttcaaggctt ctaacactgc acgataggct tcttctccat ccttagcctt
    27361 aaaagttcca aatcccaata ctggaatttc tactccatta tttaattgat aggtattcat
    27421 atgcttctcc ttctatttta ttcatcaatt atagcataaa acatttttta agtagtacta
    27481 tcttctgtaa aaaataagtc tgtcaaatat ctaacagacc tattttttct tgcttattca
    27541 tcacgactat agtacagctc atgagctttt aggcgagtat tgagcatctc tggtatggtc
    27601 acaaaggtat aaccttgatt tttcaaatac tcaatgaccc ttggcaaggc attgactgtc
    27661 ggactgtgaa tatcatgcat caaaacgata gagccgttag ctacttgatg ctgaatttct
    27721 gtcaaaatag atgcttcatt tttactcttc cagtccagac tatccacatc ccacatgata
    27781 aagctcaaat ccaagctatt gcgaatatca tctgtaatag caccataagg tggacgcatg
    27841 agtttagaac tagaacccag cactttagtt agcacatcct cagtatcagt aatctgcttt
    27901 ttagcttcat caagagagag ttgcgagaga atcggatggc tccaactatg gtttccaaca
    27961 acatgacctt cagattttat cctcttcacc aagtcctcat tcccagaaac atttttccca
    28021 agcacaaaga aagtcgcttt aatatcatat ttagctaggg tctctaatac ctgcggggtc
    28081 gttgctggat ttggaccatc atcaaaggtt agagcgacaa ctttttgata tttcttatca
    28141 aagtaagatt ggtacaaggc cgcatctttt tcgagtaagt acgaagattg gataacatca
    28201 aagaaagcag atactggcaa ggctatctct tctaaatttt caaccacagg acttggataa
    28261 aggataatct gactatcctt gtaatcaaaa ttccatgcag acaagtcttg gtcagagaag
    28321 ttttttacaa tctgctcact ctggtcttgc tctatttttt tatcctctat gaaggaggtc
    28381 aactctttta tcagctgttc cttagcctta ctaacatctg aaaacagttg gtcaagtgta
    28441 aaaggttgcc catcttctgt taagtgaacc tttcctagac tggtcttttc agtctcttca
    28501 accttaaaag caactaaatc atagatttgt ttggtcacat tacgattaac gactcccttt
    28561 aaacctgact cttcttgctc tgtatagtag aaaacaagat tgtccttact ttctagcttg
    28621 tccttaacat cttgatttat caactcccta acagaggaaa tcactttctc cccttggaga
    28681 ggataatagg caatcacttc ggcttgcccc tgacgaaaat gctccttctg atttccctca
    28741 ctcaattgat catctttctc ttttttgagc gattcaatct tttgctcaaa acttttttgc
    28801 tggtagatct taaaagcaat aaatccgcct aataggcaaa tagaaattgc taaaatacca
    28861 atcaaagcca ataatacatg tctcgttttc ccgtgtctgc cacgtcctag tctactttta
    28921 ttcatattta tatcataaca aatttgagca ataatttcaa tgatacaatt tggcaatcca
    28981 caattcctct agcaagccaa agcatcttac atcaactcca acatccgtat ctgtagagat
    29041 tatgtagttt tctatctatg gaatttctag tagattgaag ctagaatagt agacctatac
    29101 ttctaaaaca ttgttagaaa tcgatttgat tgtcctgatc gatttgtcct gttcttattt
    29161 cattttacta taaaactatg agaagctagg tcatctgaga agtaattact atcgacaatt
    29221 gcgatagcgt atactggtgc aatttgtttg tagctctggt gggtatcacc ttcacgttgg
    29281 cgaatttttt ctaggttttg attaacctga ctgcacagat aagcccatag gcgattgatg
    29341 aaaaaattct gatgatgaac ctgaatttcg atgataactt gggttccatt atctaactcc
    29401 gccaagacat ctatactagt atagaaatcc tgcgctgagt agggcatgga aggcaagacg
    29461 tgaatgttac ttccctccaa aatcgtcaca ttttttgctg gcaagtccag catatcgcgg
    29521 ataaattgac aaagcattag ttgggctgat acccggatgt ctgagaatcc tccttttcct
    29581 cctttttctc cttttcctcc ttctattata gcacattttt aaatctaggt ttgctagatt
    29641 ctatgcttct atctatttat tcggaaaaga aagcttttct tcagaagaaa gctaatagct
    29701 tatttgaagt tttctcctct ctgttggata gtcccgtcta tctatgttat aatgaaagaa
    29761 ggatttaaaa tcggaaaagg agtatttatg ctaaaattag gtgtcatcgg aactggcgct
    29821 atcagccatc atttcataga agcagcccat accagtggag aataccaact ggtcgcaatc
    29881 tattctagaa aactagaaac tgcagcaacc tttgcttctc gctatcagaa tatccaacta
    29941 ttcgatcaat tagaagtctt cttcaaatct tcttttgatt tagtctatat cgccagtcca
    30001 aactccttac attttgctca ggcaaaagct gccttgtctg ctggtaaaca tgttattctt
    30061 gaaaagccag ctgtcactca accacaagaa tggtttgatt tgattcaaac agctgaaaaa
    30121 aataactgtt ttatctttga agcagctcgt aattaccacg agaaagcctt tactactatc
    30181 aaaaactttt tagcagataa gcaagtgtta ggagcagatt tcaactatgc caaatactct
    30241 tccaagatgc ctgacttgtt gactggacaa acaccaaatg tcttttcaga ccgttttgct
    30301 ggtggagccc tcatggattt ggggatttat cccctctatg ctgctgttcg tctttttgaa
    30361 aaagctaatg acgctactta ccatgctcaa cagcttgaca atagcattga cctaaatgga
    30421 gatggtatcc tcttctaccc agactatcaa gttcacatca aggctggaaa aaacatcact
    30481 tccaatcttc cttgcgagat ttatacaaca gatggaacct tgactctcaa cacgattgag
    30541 catattcgct cagctatttt tgccgaccac caaggaaatc aagtccagct tcctatccaa
    30601 caggctcctc atacgatgac tgaggaagtc gctgcatttg cacacatgat ccagcaacca
    30661 gatctgaatc tctaccaaac ttggctgtat gatgcaggct ctgttcatga gctactatat
    30721 accatgcgcc agactgctgg cattagattt gaggcagaaa aatgaaaacc aaactaccga
    30781 ctgaatggca agaactgagt gaccaactcg gtttccaaga attcaccccc attcaaactc
    30841 aactatttga gctccttctt gctggagaaa acctcctagg agtgagccaa accggaactg
    30901 gtaagaccct agcgtatctc ctaccaagtc ttctcagact acaaaagaaa aaagcccaac
    30961 aactcttgat tctagcacca aacacggaac ttgctggaca gatttttgat gtatgtaaaa
    31021 cgtgggcaga agctatcgga ttgactgctc aactcttcct ttcaggttca agtcagaaac
    31081 gacaaattga acgtcttaaa aaggggccag aaattctgat tggaactcct ggccgtatct
    31141 ttgaactcat taaacttaaa aaaatcaaga tgatgaatgt ggaaaccatc atcctggatg
    31201 aatttgacca attactagat gattctcaga ttcactttgt agagaaaatc actcactacg
    31261 cacctcgtga ccaccaactg gtctacatga gtgccacgac caagtttgac caagaaaaga
    31321 ttgtgccgaa tacacgcact attgatctct ctgatcaaaa attggacaat atccagcatt
    31381 tctacatgca ggtagaccaa cgtcatcgag tggatatgct acgaaaactg gctcatgtag
    31441 aggatttccg tggtctagtc ttcttcaata gcctgtcaga ccttgggaat gccgaggaaa
    31501 aactacagta tcgtgatata ttggctgttt ccctcgctag tgatgttaat gttaaattta
    31561 gaaaaatcat cttagaaaag tttaaagata accaactaac cctgcttctt gcaactgacc
    31621 ttttggctcg tgggattgat atcgatagcc tagaatgtgt cgtaaacttt gatattccta
    31681 gagatagcga aacctacaca caccgtgctg gccgtactgg tcgtatgggc aaggaaggtt
    31741 atgttatcac tctcgtcact catccagaag aaatcaaaaa acttaaaaag tttgcaagta
    31801 tccgagaaat tgtcctaaaa aaccaagaac tctatatcaa ataaagcccc attgctctat
    31861 acacatccag tgtatggagc ttttttacaa gcaattcaat tttatactca tctgttttca
    31921 aaaagcattc tagtccatct ccgattaacg atgggcttta tcacctcctt ctccagtcct
    31981 tgtatgatat cttgaagttg attcatgaca tcttccaaag tttgaaaggc tttattctta
    32041 aatccacgtt tacgaatctc tttcaacact tgttcaatgg ggttcatctc tggtgtgtat
    32101 ggaggaataa aggtaaaacc aatattagtc ggaatcttta aggtacttga tttatgccat
    32161 atagcattgt ccataacgag taaaaggata agcttgtgaa agctcttcta aaaaggcgtt
    32221 catccactca gtattacatc taccagctat taagaaaaat gattcgcctg tatgggcatc
    32281 aacagctcca taacaatagc gaaattctcg tatatagtga ctatggacat gtggacctac
    32341 tcctattgga gaccaacaag atcccagttt actgattcta ccgaaaccag cctcatcttg
    32401 gtacatcaag cgaaccttac gaaaacgtct actatattta aatcgctttc ttgccttctt
    32461 gaattgagat tttattttta gacgcgacaa tggtttgagc gtctgttttc ctaggatgtt
    32521 ctggacgtgg cataatattt cgccagccat ggcgcttcaa cagttgatag aaggcatcac
    32581 gtgtgtagga acgacctaac tcctttttat aagcctgaaa taaggcatca attgtaacaa
    32641 attctcctgc ctctgtagcc ttcaaatgac gggcaagaaa ggctttctct tcctcaactg
    32701 tcatatatgc atggttacga ccaccacgtg tttcttgaag gagagagtcg agtccgaact
    32761 cctcatattt tttacatttt gctaaatcgt tgtttgatta cagtctaaaa gctctataat
    32821 ctctttataa gatttgccca tcagacgaaa tagaacgatt tgaaggcgtt tatgatattt
    32881 agctgtacga gagtctttta aaagtgtttt gatggtttgg atttcttctt tagttgattt
    32941 catattacta ttgtataatg ctttttgatt tgagtctagt attatactct tcgaaaatct
    33001 cttcaaaccg cgtcagcttc gccttgtcgt atagatgtta ctgacttcgt cagttctatc
    33061 tacaacctaa aaacagtgtt ttgagcagtc tgcagctaac tttctataac aaaatgaaat
    33121 aagaacaaga caaatcaatc aagacaatca aatcgatttc taacaatgtt ttagaagtag
    33181 atgtgtacta ttctagtttc aatatattat agtttgctct ttgattttca ttgagtatta
    33241 gttcaatatc taaaaaaaga accttgccaa gcaagattct tttagtgtct gacttaaata
    33301 attgttcttc tgggaactaa atcgaattaa gcttcgattt ctgtaaccat acctgaacca
    33361 acagtacgtc caccctcacg gatagagaat gtagtacctt gttctacggc gattgggtga
    33421 atcaactcaa cgtcgattgt cacgttatca ccaggcatta ccatttcagt acctgctgga
    33481 agttcgattg aacctgtaac gtcagtagta cggaagtaga attgtggacg gtagttgttg
    33541 aagaatggag tgtgacgtcc accttcttct ttagtaagga tgtagacttc acctttgaat
    33601 ttagtgtgtg ggttgattga acctggttta gcgataactt gtccacgttc gatttcatca
    33661 cgttgaacac cacgaagaag gacacctacg ttatctccag caagaccttc gtcaagttgt
    33721 ttacggaaca tttcaacacc agtaacaact gctttttgag tttcttcttt gataccaacg
    33781 atttcgattt cgtcgttgac tttaacgata ccacggtcga tacgtcctga agcaactgta
    33841 ccacgtccag tgattgagaa tacgtcctcg actggaagaa gcaatggttt gtcagtgtca
    33901 cgttctggtt ctggaatata ctcatcaact gtgttcatca attccataac gatgtcttcg
    33961 tatttagagt caccttcaag agctttaagt gctgaacctt ggataactgg aagatcgtca
    34021 cctgggaagt cgtattctga caataggtca cggatttcca tttcaaccaa ttcaagcaat
    34081 tcttcgtcgt caaccaagtc aactttgttc atgaagacga taaggtgttt aacaccaacc
    34141 tgacgtgaaa gaaggatgtg ctcacgagtt tgtggcattg gtccgtcagt tgaagctact
    34201 acaaggatag ctccgtccat ttgagcagca ccagtgatca tgtttttaac gtagtccgcg
    34261 tgtcctggag cgtcgatgtg agcgtagtga cgtttttcag tttcgtactc aacgtgcgca
    34321 gtgttgatag tgataccgcg ttcgcgttct tctggagcag catcgataga cgcatagtct
    34381 ttaggttggt taactgatga aggcaagcga cgtgccaaaa cagttgtgat agctgcagtt
    34441 agggtagttt taccgtggtc aacgtgtccg atagtaccaa tgttaacgtg tggtttacta
    34501 cgatcgtatt tttcttttgc catttgagta aaagcctcca ataaaatata ttttatagat
    34561 agacagtagg caatacagtc taactttcct tactatttta tcaaatttca gctaaattgc
    34621 aagtgtttta caacgttttt gtgaattatt cttggtaaaa atttaactaa ctaatagaaa
    34681 gtactaaata gacttatcct tcaaggagtt ttcagcatta tccatccact cacttgctga
    34741 aaatgtaaac agtctctggt ttatatagta aaatgaaaca agaatagggc aaatcgatca
    34801 ggacagtcaa atcgatttct aacaatgttt tagaagtaga tgtgtactat tctagtttca
    34861 atctactata caacctcaaa actgtgtttt gaacaacctg cggctagctt cttagtttgc
    34921 cctttgattt tcattgagta taaaattgca gataaaacga taacaatcat aggactcagc
    34981 agagtcaatg taggtcgaag aatagccccc ctaatcgctt attttatagc aaaatgaaat
    35041 agaaactgaa cattttgttg aggaaggtaa atcaattcct cacaatgttt gagaaagaag
    35101 ggaggactat tccaacttca acccactata aagcaaactt acaagatagg gaacccatct
    35161 ccgataatat tccgataata tatagtagaa tgaaaactgc acaatttgat tggagaattc
    35221 aaaccaattt ctcacaatgt tttagaaaca agggtctatt atttcaactg caacctacta
    35281 tagcccattg aagctggaat agcccactaa gacttctaaa ccactgctgg agaggagttt
    35341 gtttagagtt tctttcattt ccctaaaaaa tcaaaaagag agaaccagtc tgatcctctc
    35401 ttacttatag atggtgctca ctcaaatgtt aaaggagcta tttcttagag ataaaggaat
    35461 ttgaatacag ctactgccgc aattgctgct gcgataggtg ctactactgg tacccaagaa
    35521 taccaccatt ttgaatcgcc tttatgctca ccaagaactg attttggaag gaaagcatga
    35581 aggagacgtg gtcccaagtc acgggctggg ttcaaggcag gtcctgtagg tcctccaagt
    35641 gatgttacca aagccataac gaggaatcca agtgccaagt gagccacaga aagtcctgaa
    35701 gcagtgtgtg gagccacctg tgctttaata gccaagtcag aaaaatcaac tgtttgtcct
    35761 gcttctattg ccttttgttt catgaattga agcacttcag caccaaagaa gtttttagtc
    35821 aaaccaagag ctgcaaagaa caaaacaaat gaaccaacaa actcattaat caaaccattg
    35881 acagttgctg catatcgact ttcttttgta ccatggtcaa tacttgaaat agttgagaaa
    35941 gttcccaaga tgttatttgg tttttcagtt ttcaagtaga atggacggta tgttgccaca
    36001 actaaggctt ggccaaagat agcccccaag acttgcgcga taatgtaagg taccacttgt
    36061 gcccaaggga aaagaccgct aactgcaagc cctagagtga aagcagggtt gatgtgattc
    36121 ccagatacgt taccaaacat caaggctggg atcataaccc ccataccata accaacagcg
    36181 atgacgatcc agccactttg gtgacctttc gtacctttaa gttcaacgtt ggcaactgca
    36241 ccattcccaa gaatgatcaa aatggcagtt cccaaaaatt cagtggcata cttcagtgcc
    36301 catgtgaaat ccatttgcta gattctcctt aaaatttttt agatattcct tattttatca
    36361 atttttaccc tatttagcaa ctgatttctg gaaatatttt taaaaaaata ggattcatag
    36421 tttcccagtt caacgtaatt gtaagcgttt ttattcttca cgaaagacct cattctcaaa
    36481 accaggaaaa agcccacatc tgtgatcttt tccttgttcc tagattctaa aatctattat
    36541 ttttcatctt cttttttctt gaagaatcct aatcctagac cagctaacaa ggtcatgata
    36601 ccagcactag ccaaggtagc attagcttct gtacctgtgt ttggcaattc ctctctctta
    36661 cctgtctcat aagtcggaac ttctgggtct ggattcactg gagtttcagt ttttggagta
    36721 cctggttctg gagttggttt atctggtgtt gataaacggt cataccttac cgttatttct
    36781 ttatcactag agtctgacgt aacttcttgt gattcaactg ttggaatatc tggatctttg
    36841 tacttgtcaa tcttaccaga tataacctcg tcccagtttc ctgttgtcca ttcaccgtag
    36901 gttacaactc ccgtgacctt gttctcagtt tttgtacggc ttaaggttac aggttgaaca
    36961 acatcttttt ttacattttg gttcgtaact ttatcaacgt aatgaatgat acgcgttata
    37021 gtcttcgtct cagtagaggt tgctgttttg ggaaccactt ttcctcaaca ttctcacggt
    37081 agtaatagtc aactgttgca ccgtcttctg gtacgcattt gcaggagttg ctaccaaggt
    37141 gtatgttgtt ttccttgtga taactcggtc ttctttgtcc tcagttgttg ttttcccttc
    37201 aatagttttt gattctgtgg tatactcaga acctatcgct aaatcagctt ttataacaga
    37261 ctctgccaac ttctcttggc taccttcttt atagtaattg gctgttactg tagcagtggt
    37321 tggcgcttcg ctttctctat aaactaaggt cactgttcta cctttgctta caatattccc
    37381 agttaaactt gcagaatttg tatctacttc tttaaaagta taatattttc cgtcagtagt
    37441 agtcatgcta ctgagttttt tatctgtgac attatagctg gtaccaatca gttgtttttt
    37501 attggtaatg taggttccgt cactttcttt ttctccaatt ccagtatcat ttttcaatga
    37561 tagcaaacgc ccttgttcat caacatagcg aactttcaca ttttctgaga ttagttctgc
    37621 caattctgag gtttttttct ttttcttgat ttcttcggtt attttccctt tctcttcttc
    37681 gggaatattt agttttggaa tgattttttc aacaacggtt cgtgatggtt ccacagtatc
    37741 ttggatgact gaaaagtcag ctagaattgg gagattatac tgaacacggt gactttgagt
    37801 gtttactcct actctttcat tattctctga aaatacttgt acggtataag aaacaacatc
    37861 ttttcctaat agaacatccc cagtagagaa atagccgcct tttcctagtt tgctatctcc
    37921 agagcccact tctttcctaa tcttatcaga tagtttttta ccagtcagta cattcgttcg
    37981 cacaatccct ttgtctaccc ctacaaagtg ggagaatttt ttgaactctt cagaaccaga
    38041 tctagcccaa ccattattaa gggcatttgc ttttgtattt gtattctctc tcaaaggttt
    38101 ggcgattaga attatattca tcggcgctta gagttgctgc tatatctgac tcttgaatac
    38161 caacttcctt actaccattt ctagcggcag tatatgtgta tttatctgtt tctacttcta
    38221 gctgattatc cttaacatga taggtaattt tctcctggta taagagtctt cagtagttta
    38281 tatgctctgt catcatcttt agctttagaa gcagctgagt ttagccaagt tggggtatta
    38341 ggagtatctt tatcagcctg tttcaagaca ttattcaagt catttgctag cctattggtt
    38401 atcctcactg tgaaagtttt cttccagtct gaaccacttt tattagactt agatatgctc
    38461 atgaaatcct ggtcaacatt caacacaata tcgccttgtt ttttagcatc ttccgttgct
    38521 tctgcttcaa attgatcatg gagtttttgt tgctctggtg tatagttttt ttatttacac
    38581 gatttaatgt actatttttt attttagtat taagaactcc attaatacga tttttaaatt
    38641 ctgcttcaaa aaatccctta atttgttggt gcgcaagttc aacattcaaa cctttcttat
    38701 acaagtcttc attctgatac aagtcttcat tctgaacgat ttcggctaca cggtgaatgg
    38761 tttgattgta taccgtcttc tcttaaccag cagtatcctt agttttaatg actactcgat
    38821 aggttccttt agaaccgtta tagtcatcta gttttaaaaa agcattccaa acattcaaaa
    38881 atagttggaa cttcccttaa taaatccgtc actttctttt tcaagatagc ccaagcttgc
    38941 tcaataggat tcaaatccgg tgaataaggt ggtagaggta agaaaaagtg tttatcttgg
    39001 atgcagagtt catccaaaat gttcttggga tgaaaactag cattgcccat gacaataaca
    39061 tgaggtgtct tcaaagcagg taggagttgc gttttgaacc actccacaaa gaaatcgctt
    39121 gtcatgcttt tcttgtaaat catgggagct ataaactctc cgtctacttg tcctgcaaca
    39181 attgaagttc gctcaaaacg acgtccgcta atcttttcat agactttctc ccctctagga
    39241 gcccctgcat aaggacgata gaggtagcgg tcgattcctg tttcgtcaat atatacgatt
    39301 ggtaaatctt ttaggttatc caaaatatca agaaactcag cgactttctc tggctcttgt
    39361 tccttaaagc tcgtcatctt tttttaaagt gaccttaatc tgctttaaag ctgcccatac
    39421 tgaaggaaca gcacaatcaa aatgtgccgc aatttcccgt aaaaaagcat ctggatgagc
    39481 ctctacaaag gttttcaatt cctctaaagg aatctttcgg tttttgacga ctcgcttttt
    39541 cctctctatg tgttcttgtt cacatctttc ttttcccacg tgaagagagt tctgacgcca
    39601 acaccaaaaa acttggctgc ctcgacatgg ctgtgcccct ctttgatgta atctaatgct
    39661 cgctgtttaa aatcagtact atatgccata gctttattat aatatgcttg tttaaaacta
    39721 agcaactata tcaatgtttt gttatgagaa gacttacttt ttttactgaa attgaatttt
    39781 tcctagcctc aacatcatat aaccccaaaa ttcaatctga cctggtttga agagatgttc
    39841 gaagcagata aggttttact aaaactaaaa aaaatgatat aatcaaactg attaaatgtt
    39901 ttaaaaataa aggagaattt gaatgtctta ccaagaaaat taccagaaat gggttgattt
    39961 tgtggagctt cctgactacc ttcgtcaaga tttggaaaat atggacgaaa aaactaagga
    40021 agatgccttc tatacaaatc ttgaatttgg tactgcaggt atgcgtggct tggttggtgc
    40081 tggtacaaac cgtatcaaca tctacgttgt tcgccaagct actgaaggat tggctcgttt
    40141 gattgagtca aaaggtggaa acgagaaaga acgcggtgta gcaattgcct acgatagccg
    40201 tcacttctca cctgagtttg cctttgaatc tgcggcagtt cttgctaaac acggcatcaa
    40261 atcttacgta tttgaaagcc ttcgtccgac tccagaacta tcatttgcag ttcgtcatct
    40321 caactgtttc gcaggtatca tggtcacagc cagccacaac cctgctccat ttaacggtta
    40381 caaggtttac ggtgaagacg gtggacaaat gcctccacac gatgcggacg ctttgactac
    40441 ttatatccgt gcaatcgaaa acccatttgc agttgaagtt gctgatgtgg aaactgaaaa
    40501 agcttctggc ttgattgaag ttatcggcga agctgttgac atagaatacc ttaaagaggt
    40561 taaggacata aacatcaacc cagccttgat tgaagaattt ggtaaagaca tgaagattgt
    40621 ctacacacca cttcatggta ctggtgaaat gttggctcgt cgtgctcttg cccaagcagg
    40681 atttgactct gttcaagttg ttgaagcgca agcaactgct gacccagact tctcaactgt
    40741 aacatctcca aacccagaaa gccaagcagc ctttgccctt gctgaagaac ttggtcgtca
    40801 agttggtgca gatgttcttg tcgcaactga cccagacgct gaccgtgttg gtgttgaagt
    40861 tcttcaaaaa gatggtagct acctcaacct ttcaggtaac caaatcggtg ctatcatggc
    40921 taaatacatc ttggaagctc acaaaaacgc tggaactctt cctgaaaatg ccgctctctg
    40981 caaatctatc gtttcaactg acttggtaac gaagattgct gaaagctacg gcgcaactat
    41041 gttcaacgtc ttgacaggtt tcaaatttat cgctgaaaaa atccaagaat tcgaagaaaa
    41101 acacaaccac acttacatga tgggatttga agaaagcttc ggttacttga ttaaaccatt
    41161 tgtacgtgat aaagatgcca tccaagccgt tcttgtcgtt gctgaacttg ctgcctacta
    41221 ccgttctcgt ggtttgacac ttgctgacgg tatcgaagaa atctataaag aatatggcta
    41281 ctacgcagaa aaaacaatct ctgttactct ttcaggtgtc gatggtgctg aacaaatcaa
    41341 agcgattatg gctaaattcc gcaacaatgc tccaacagaa tggaacgcaa cagctatcac
    41401 tgtcgtagaa gacttcaagg cacaaactgc tactgttgct gacggtactg ttacaaactt
    41461 gacaactcct ccaagtgatg tgttgaaata cacacttgct gacggttcat ggattgccgt
    41521 tcgcccttca ggtacagaac caaaaatcaa gttctacatt gcagttgtag gggaaaccaa
    41581 cgaagaatca caagctaaga ttactaacat cgaagcagaa atcaatgcat ttgtaaaata
    41641 aagacctata aaagatgaga ccaaccaacc tcatcttttt tcgtatcaaa tctaagcaat
    41701 tttagaaact ttatggcata ctagacttat caatgaaagc gaggtaatct catggaacaa
    41761 tttttagata acatcaaaga ccttgaagtc actacagttg tgcgtgcgca agaagctctt
    41821 gataaaaaag aaactgcaac cttctttatc ggtcgcaaaa cttgccctta ctgccgtaaa
    41881 tttgcaggta cattgtcagg tgtcgtagct gaaaccaaag ctcacattta cttcatcaat
    41941 agtgaagaac caagccaact caatgatttg caagcattcc gctcacgcta tggaatccca
    42001 actgtaccag gttttgttca tattacagat ggacaaatca acgtccgttg cgactcttca
    42061 atgtcagcac aagaaatcaa agatttcgca ggattgtaaa aaatcagcta acaagtctta
    42121 aaatcacaaa aacgcataat gtaaggtgca aaaaccttga tattatgcgt tttattgttc
    42181 ctaaaacagt tacatcagca tttcattaca gaacatttta attttggaag tcaaatcaat
    42241 ttttaacaat gttttagaaa tcacagtggg ctattccata ttcaattatc cagattcaat
    42301 cgctattatc gttttcctag attacaaaaa aactacgttt tgctaatcaa aacgcagttt
    42361 cagccactat tttatcttac tgtccttctt ttacttcttt ggttgctaca tcttctccaa
    42421 accatttttg gctgatttct tggaacttgc cgtccttgta aagactagaa aaagcttcat
    42481 ttatcttctt aaccaagttt gtatcttcct tacgggctcc aaccgcaaaa gcttctgttt
    42541 ctagtccaac tgtaaagaca ttataatcgt ttaaaacacc ttctgcttct aaataatagt
    42601 ttgcatagac acggtcaatc aatagaccat caattcgatc gtttttcaaa tcaatcaagg
    42661 cttcattaaa ggtttggtat tgattcgctt ccttattagc gacaatattc ttcaaaattt
    42721 ctggatttgc ttcaaagtcc gcataaccag atgaaccagc ttgagctcct aatgtctttc
    42781 cagtcatatc ctttgcagtc gtgataccag atgatttctt cgtaaccaat acctgctcat
    42841 tcttcatata tgagttactg aaagccacct tttcacggcg ttcgtctgta gcggaatagc
    42901 cattccaaat cagatcaatc gttccttttg tcaattcagc ttctttcaaa tcccaatcaa
    42961 tcggttgcca atttaccgtg attccgtatt tttcaaaaac agctgtagct aaatcaatat
    43021 caaatcctgc ataagaacca tctttctgag caaatcccat tggaacaaaa gtactatcaa
    43081 atccaatagt aatagacttg ttagactggt actttgacca attatctcca ctagtttcgc
    43141 tagaattttt cccacaagct actaagaaca aagcagtcat cagactgact aatacaagca
    43201 tccatttttt catccatttt tcctcctatt taggttctac tttcaataat acatctgcga
    43261 tattttcagc aaactgcaaa tcatgggtaa ccacaatctg ggtcatccca agttccctat
    43321 tttgcaagat tagcttctcc acttccaaac gtaattctgg atccagggca gaagttggtt
    43381 catcgtagcc aatgatttct gggtcaatca tcatagcacg cgccaaagcc acccgctgct
    43441 tttgcccacc agatagtgag aaaggatagg cctctgcgtg tcctcctagt cctaactgtt
    43501 ccaagagtcc actcgccttc ttctcagcct cttcctgctt cattcccatg gtcttcacag
    43561 gcgataaagt caaattttcc agaactgata aatgagggaa cagttgaaaa tcttggaata
    43621 caaatcccag tagattgcgc ttctgcaatt catccagctc taaaggttgt ccattataaa
    43681 agatttgccc tgaatcaatg gtttcaagac ctgcaagcat acgtaaaaga gttgtcttac
    43741 ctccaccaga aggtccaacg atagccagga tttgcttttc aggaatactt agactgaaat
    43801 tagacaggat ttgtttgtct ccaaagactt tattgatatt tcgtaattct aacatggcag
    43861 cctcctatct ataataacta tacttcttct caactttttt ggaaataatt gtcacaatcc
    43921 caatcaaaat caaataaatg gctcctgcca agaacatagg aactagacta gcatcgcggt
    43981 tagcagctgt tcgactagcc aagataaggt ctgaaattcc gagagcatag accaaagaag
    44041 tatccttgac caaactcata acttcattaa agacactagg aagaacgatc ttggtcactt
    44101 ggggcaagat aatatagcgc actctgtcaa aagggctaaa cttcaagacc ttggcggcct
    44161 catactgtcc tcttggaata gtgtcaattc ccccacggaa aatttctgca aagtaagctg
    44221 catagttgag aacaaaggca ataatagctg caggaaggcg gtctaaacga atcccaatac
    44281 ttgggagcac ataatagata aaaatcagtt gcaagagtaa gggggttcct cgcataaccc
    44341 agatgtaaat gttaatcaga taatggaggg gcttccaatg gacttgcaag gcaaaggcaa
    44401 tcaaaacgcc caagggaatc gaaaatagca agaccagtgc aaagacctgt acagtcgtgc
    44461 tcgcaccatt cagtaaactc ggtaatatct caaataaata agacatactg cacctcctaa
    44521 aagtaattgt tcctattata gcatgaataa acaagattac caagtatttt tgtaaaaaga
    44581 ttctaccata tacaaataaa aaccgtagct atcaaaagag ataaactaca gttctatatg
    44641 tttaaacatt cttacaatct ctcaaacagt tcttgcattt gcacatcatc tggaaccagt
    44701 tttaagtaag tgtgagcatg gacttttgct tcttcaaaat gtcccaattc acgcaagaga
    44761 tagatatagt gttccagaaa ttctggattg tccttcaaat ctcctgtcaa ctcttgataa
    44821 tgctcataag cagtatccaa atcgtccatt tcttgataag aacgagcaat catccacttg
    44881 gtcaaaagat tttctggctc ctcactctgc aagtctagaa tatcctcata acgctcctgc
    44941 tccagataaa tagtggctaa acgaagcaag atttcttctg tatcctcagc gtcttctttt
    45001 gcagcaagaa gataattttc tgcaccacta gcatcatgca attcataaga aaattgtgaa
    45061 gcagctagca agaggcgagt ttcaaaggga tttttctcta atccttgctt agcgatacgc
    45121 agggcttctt gaacttgatg ttccttatgt aaagcctgac tgtacccata ctcatagcct
    45181 tcaaagtcag gagaaatggt atcaagctgc ttaaagtaga gggtggcttt ttgatattct
    45241 tcttgatcaa aataaagatt tgccaactca aaagctgtta agtcatcgta ttctaactcc
    45301 agggcttttt ctaaaaactc agtagccgtt tcaaatttcc ctaactgagc ataggcaaag
    45361 ccaattcgtt gataggtgga aatgcccgtt tgctcataaa tcgagcgatt atctaactgg
    45421 gcataggctt gaatagccgc ttggtaattt tccaactcac tatccaactc tgccaaaccc
    45481 aatatcaaga gagaatcctc tgagtaggtc aaggcctcca ataatttctc acgtgccaca
    45541 tctgtcaaac cttccagctg gtaaaggtct gccttcagag ccaaagacga gacataccag
    45601 tcactgtcag cttggatttc ctcaagatag gtaaaggctt cttctatttg accatcctcg
    45661 ctagcaattg cagctagatt aagatgaacc tctggaaaat cctctacaat tttcaggtaa
    45721 atttccttgg cctgaggata gaaaccaatc ccttcaagat aagttgccaa ctcatacaga
    45781 agatcacttg aatcattttc taaagctttg gcgaaataat gctcagcctt agttaaatct
    45841 tgctcctcca aagcctgtaa catctgttga ctattgttca ctcttgactt cctcaccctc
    45901 taattcatat agaccactga ctaccttata ccattcgaaa atggctgaaa tgacaacctt
    45961 agcagaggca taaaccggaa taccaagtaa aactccccag ataccaaaca tagatcctga
    46021 agttaacaaa acaaagagaa cattaatagg atggatgttt aattgacttc ccaaaatcaa
    46081 tggagagaca aaacggcctt caatagtttg ttctacgata aagacaatca ctactttcaa
    46141 aagcatgact ggaccagcaa tcaaacccaa tactagagca ggaagcatgg ctagaaagct
    46201 accaagataa gggaccagat ttaaaatacc agcagtaacc cccagcgtaa ccgcatagcg
    46261 tagaccaata atcttgaaga agatgataaa cattactgct acaataatag ccactgtcac
    46321 ttgccctcga acatagttgg acaactgttg attcacatct gatagaactt gtccaacagg
    46381 ttccttcaat tttcttggaa tgaattgggt caaatagtta cgcaagcctt tcccatcacg
    46441 caagagataa aagagcatga aaggaacgat aatcaaggca acaatcactt gagaagctcc
    46501 gctaataaag gcactcaccc agttgactgc ctgagatgaa accttacttg ccaaaactgt
    46561 agcctggcta gaaaaattgg tcaaaacttg ctctaattga ggtctgaaat catctggcag
    46621 gtgctgggct accaatccat taacaatcct atctatatct tctaagtaaa caggaacgtt
    46681 tcttgcaaag gtcaaaacct gacgttgcag atttggaatg gcgactgcca agccccaaat
    46741 gataaagaga gcgatgataa caaagacaat agtgatagct ataacacgat taaccttatg
    46801 cttctccatc caatcaacaa taggattcaa caaataatat aacaaaccag acaaaatgac
    46861 tggcaacatc acaactgcca aaaagtctaa aacaggtgaa aatagaaaac taatcttact
    46921 taaaataaaa agattcagtc ccaataataa ggttactaaa aataccgtaa ttgccttgtt
    46981 atctaaaaac cacttgaaaa accaagatag gctaaaatgt ttctcttttt gctccataaa
    47041 tacttccttt ctattgttct ctcattatac catttttgaa acaaaataac tcttttttaa
    47101 agtgcttaca tctaattgga tatctttaat ttacttgttt ttgtgatata atagtcttat
    47161 ctttacacag aggtatagta atgaaagaaa ctgtttattt tggaacttat acacgtcgta
    47221 cttctcaagg gatttacaag gcagactttg atacagaaac tggtcagctt tcaaatctag
    47281 aactttttgc agctgagccg agtccaacct accttgcctt tgaccagcac caacatttat
    47341 acactgttgg tagccaagac gataaggggg gaattgcagc ctatcaaact gacgggactg
    47401 tgttaaatca tgttgttgaa gaaggagctc cccactgtta tgttgctgtc gatgaaaagc
    47461 gtgatttggt ttacgcagcc aactatcaca agggacaagt ccttgtttat aaacgccagg
    47521 aagatggtag tcttctactt agtgatatgg atcaacacag tggccaaggt ccacatgaaa
    47581 atcaagcttc cccccatgtt cactatacag atttaacacc tgaccactat ctagtgacct
    47641 gcgacttggg aactgaccaa gtcatcacct atgacctcga tcaagaagga aaattatcta
    47701 agctctatac ctatcacagc aagccaggag caggctcacg ccatatcatt ttccataacc
    47761 actataaaat cgcttatctc atttgtgaac tcaatagtac tatcgaagtt ttaatctacg
    47821 atggcgttgg cgaatttgaa cgtatgcagg ttatttcaac tttaccagaa gcttacgaag
    47881 gctttaatgg aaccgctgct attcatctct ctaaagacgg taaatacctc tacgcttcta
    47941 accgtggcca tgattctatc gcagtatata ccatccttgc ggacggtagc ttagagttat
    48001 tagaaatcgt tccaacgcat ggtcagactc cacgtgattt tgatttgaca cccgaccaaa
    48061 aatttctcat tgttgtccat caagactctg acaatgcaac tgtctttaaa cgtaattgtg
    48121 acaatggtcg tctagcagaa ctctccaacg acttccatgt tcccgaagca gtctgcatcc
    48181 gttttgctcc ttaaaacacc ataaaaaatg aacttggtat tttggctcta taatatttgt
    48241 agtgggtaaa tcca
//
LOCUS       DAVLYV010000007        45880 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLYV010000007 DAVLYV010000000
VERSION     DAVLYV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45880
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.007"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(98..2113)
                     /gene="recG"
                     /locus_tag="VYO70_000395"
     CDS             complement(98..2113)
                     /gene="recG"
                     /locus_tag="VYO70_000395"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1712684.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLQTIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKSIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFACKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(2132..3235)
                     /locus_tag="VYO70_000396"
     CDS             complement(2132..3235)
                     /locus_tag="VYO70_000396"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1712685.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(3225..3593)
                     /locus_tag="VYO70_000397"
     CDS             complement(3225..3593)
                     /locus_tag="VYO70_000397"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1712686.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(3632..4663)
                     /locus_tag="VYO70_000398"
     CDS             complement(3632..4663)
                     /locus_tag="VYO70_000398"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1712687.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            complement(4665..5696)
                     /locus_tag="VYO70_000399"
     CDS             complement(4665..5696)
                     /locus_tag="VYO70_000399"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1712688.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(5777..8290)
                     /gene="secA"
                     /locus_tag="VYO70_000400"
     CDS             complement(5777..8290)
                     /gene="secA"
                     /locus_tag="VYO70_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1712689.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDIITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(8436..8723)
                     /locus_tag="VYO70_000401"
     CDS             complement(8436..8723)
                     /locus_tag="VYO70_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712690.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(8751..9383)
                     /locus_tag="VYO70_000402"
     CDS             complement(8751..9383)
                     /locus_tag="VYO70_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1712691.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKFDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            complement(9387..10028)
                     /locus_tag="VYO70_000403"
     CDS             complement(9387..10028)
                     /locus_tag="VYO70_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712692.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMILYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKV"
     gene            complement(10021..10812)
                     /locus_tag="VYO70_000404"
     CDS             complement(10021..10812)
                     /locus_tag="VYO70_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712693.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIVTYFFGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLEKYEDNV"
     gene            complement(10997..11332)
                     /locus_tag="VYO70_000405"
     CDS             complement(10997..11332)
                     /locus_tag="VYO70_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712694.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYIPLY
                     ALFSLSWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            complement(11472..11807)
                     /locus_tag="VYO70_000406"
     CDS             complement(11472..11807)
                     /locus_tag="VYO70_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712695.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWIYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            complement(11898..13208)
                     /gene="der"
                     /locus_tag="VYO70_000407"
     CDS             complement(11898..13208)
                     /gene="der"
                     /locus_tag="VYO70_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1712696.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPGMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(13222..13935)
                     /locus_tag="VYO70_000408"
     CDS             complement(13222..13935)
                     /locus_tag="VYO70_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1712697.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLKQKKLL"
     gene            complement(13932..14828)
                     /gene="dnaI"
                     /locus_tag="VYO70_000409"
     CDS             complement(13932..14828)
                     /gene="dnaI"
                     /locus_tag="VYO70_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1712698.1"
                     /translation="MESVGDVLKRQPSRFHYQDLVQKIMKDPDVAAFVQQESLNQDEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVMNHGYADVSYEETPELIAAEKEAA
                     IKKRLNLINFPSSLKNVSFLDVYRDDVQRLTVLKRMIEFVNDYPNNLKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAIGDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKGKNGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(14829..15998)
                     /locus_tag="VYO70_000410"
     CDS             complement(14829..15998)
                     /locus_tag="VYO70_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1712699.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPEYKNTSSAEELEEMERQTLELLAKLDNGGD"
     gene            complement(15999..16472)
                     /gene="nrdR"
                     /locus_tag="VYO70_000411"
     CDS             complement(15999..16472)
                     /gene="nrdR"
                     /locus_tag="VYO70_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1712700.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            16612..16977
                     /locus_tag="VYO70_000412"
     CDS             16612..16977
                     /locus_tag="VYO70_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1712701.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            16982..17677
                     /locus_tag="VYO70_000413"
     CDS             16982..17677
                     /locus_tag="VYO70_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712702.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            17694..18461
                     /locus_tag="VYO70_000414"
     CDS             17694..18461
                     /locus_tag="VYO70_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712703.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(18814..20013)
                     /locus_tag="VYO70_000415"
     CDS             complement(18814..20013)
                     /locus_tag="VYO70_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712704.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(20010..20903)
                     /locus_tag="VYO70_000416"
     CDS             complement(20010..20903)
                     /locus_tag="VYO70_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712705.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            21019..21099
                     /locus_tag="VYO70_000417"
                     /pseudo
     CDS             21019..21099
                     /locus_tag="VYO70_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            21584..21797
                     /locus_tag="VYO70_000418"
                     /pseudo
     CDS             21584..21797
                     /locus_tag="VYO70_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            22212..22829
                     /locus_tag="VYO70_000419"
     CDS             22212..22829
                     /locus_tag="VYO70_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1712706.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(22872..23759)
                     /locus_tag="VYO70_000420"
     CDS             complement(22872..23759)
                     /locus_tag="VYO70_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1712707.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(23908..25791)
                     /locus_tag="VYO70_000421"
     CDS             complement(23908..25791)
                     /locus_tag="VYO70_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1712708.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            26168..27622
                     /locus_tag="VYO70_000422"
     CDS             26168..27622
                     /locus_tag="VYO70_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1712709.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFRETGVKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFQGQYYAIVGGQDLEKKGFVRLYKAVNNDYTNWQAVGNLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSHAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            27603..28568
                     /locus_tag="VYO70_000423"
     CDS             27603..28568
                     /locus_tag="VYO70_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1712710.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(28603..29877)
                     /locus_tag="VYO70_000424"
     CDS             complement(28603..29877)
                     /locus_tag="VYO70_000424"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1712711.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMAILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VVLSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(29877..31049)
                     /locus_tag="VYO70_000425"
     CDS             complement(29877..31049)
                     /locus_tag="VYO70_000425"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1712712.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(31180..31428)
                     /locus_tag="VYO70_000426"
     CDS             complement(31180..31428)
                     /locus_tag="VYO70_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712713.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(31496..32100)
                     /locus_tag="VYO70_000427"
                     /pseudo
     CDS             complement(31496..32100)
                     /locus_tag="VYO70_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(32162..32611)
                     /locus_tag="VYO70_000428"
     CDS             complement(32162..32611)
                     /locus_tag="VYO70_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1712714.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYNYIKTHRVS"
     gene            complement(32613..33371)
                     /locus_tag="VYO70_000429"
     CDS             complement(32613..33371)
                     /locus_tag="VYO70_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1712715.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(33486..35465)
                     /gene="pknB"
                     /locus_tag="VYO70_000430"
     CDS             complement(33486..35465)
                     /gene="pknB"
                     /locus_tag="VYO70_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1712716.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIILTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(35462..36202)
                     /locus_tag="VYO70_000431"
     CDS             complement(35462..36202)
                     /locus_tag="VYO70_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1712717.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            complement(36217..37530)
                     /gene="rsmB"
                     /locus_tag="VYO70_000432"
     CDS             complement(36217..37530)
                     /gene="rsmB"
                     /locus_tag="VYO70_000432"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1712718.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFANGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(37523..38458)
                     /locus_tag="VYO70_000433"
     CDS             complement(37523..38458)
                     /locus_tag="VYO70_000433"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1712719.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(38471..40867)
                     /locus_tag="VYO70_000434"
     CDS             complement(38471..40867)
                     /locus_tag="VYO70_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1712720.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QESNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKVYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(40933..41247)
                     /locus_tag="VYO70_000435"
     CDS             complement(40933..41247)
                     /locus_tag="VYO70_000435"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1712721.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(41272..41898)
                     /gene="gmk"
                     /locus_tag="VYO70_000436"
     CDS             complement(41272..41898)
                     /gene="gmk"
                     /locus_tag="VYO70_000436"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1712722.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(42028..43641)
                     /locus_tag="VYO70_000437"
     CDS             complement(42028..43641)
                     /locus_tag="VYO70_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1712723.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSHEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(43832..44086)
                     /locus_tag="VYO70_000438"
     CDS             complement(43832..44086)
                     /locus_tag="VYO70_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV1712724.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(44090..44344)
                     /locus_tag="VYO70_000439"
     CDS             complement(44090..44344)
                     /locus_tag="VYO70_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1712725.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(44582..45679)
                     /locus_tag="VYO70_000440"
     CDS             complement(44582..45679)
                     /locus_tag="VYO70_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1712726.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILARLGIDSLAFGTEEVLD
                     YQKIADLYTEKGAEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVEAVATKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETS"
BASE COUNT        13052 a        10458 c         8797 g        13573 t
ORIGIN      
        1 catccacaac ctcaaaacag tgttttgagc aacctgcggt tagcttccta gtttgctctt
       61 tgattttcat tgagtataag ttttccttag agaaagctta atccagatgt tctttctttt
      121 ccagatgaag ggcaatcatg cgccactctg gatcttcttg ccaagcttct atagaactaa
      181 tgtagctagc aacctttctt gcttcttcta aaatcggaaa atcttcgata atatcagcca
      241 cttggaactc tggaagtcct gactgtctgg ttccaaaaat ctcaccagaa ccacgcattt
      301 tcaaatcttc ctccgcaagg acaaatccat tggtcgtttc tgtcatgatg cgcatgcggt
      361 ctttcccaga atccgtcttg ggattggcaa cgagaacagc gtaggactgt ttgtcccccc
      421 gaccgacacg acctctaagc tggtgaagtt gactgagacc gaagcgatcg gcatccatga
      481 taatcatgac agtcgcattg ggaacgttga ccccaacctc aataaccgtc gtcgaaacca
      541 gaatatccgt ctttctctcc ttgaaatcct gcatgatctg gtctttttcg tcactcttca
      601 tcctaccatg tagaagagcc acctctgcct tgcatgcaaa atgagtcgtc aactcctctg
      661 ataaggcaat ggcatttttc aaatctagag cttctgattc ttcaatcaaa ggagagatga
      721 cataggcttg ggaacctttt tgaatttccc cctctaacca agtcaagacc tgaggtagtt
      781 gctcatgttt gatccagcgc gtcacaatag acttccgacc tgctggcatc tggtctataa
      841 tggaaacatc catatctcca aaggctgtga tggcaagcgt ccgtggaatg ggagtcgccg
      901 tcatcatgag gacatctgga ttgtcacctt tttcccgtaa aatacgcctt tgccctacac
      961 caaaacggtg ctgctcatcg ataataatca aaccaagacg agcatactcc accccatctt
     1021 gtatcagagc gtgagttcct ataatcaaat cagcctcacc cttggcaatg gtctgcaaga
     1081 cttctctctt ttctgcagct ttcaaggaac ctgtcaagag agccaatttc aaattgggaa
     1141 aaaggttctg taaactctca aagtgttgct ctgcgaggat ttctgttggt accattaggg
     1201 cagcctgata acctgctgtc actgccgcaa acatggccaa gccagcgact accgtttttc
     1261 cactccccac atccccttgt aggagacgat tcatgtggtg gtcggacttc atatcagtta
     1321 aaatttcctg caaactcttt tcctgagctt gggtcagggc aaaaggaaga cttactttaa
     1381 ctgctgtcac tttttcctga gaccaattca gaaccagacc acttccctga actctatttt
     1441 cagacttgag cgtctgcaac tgcatttgga aataaaagag ttcctcaaac ttgatacggc
     1501 gaagagcctg cttgtattct gccaaatcct ttggaaaatg catagctcgg actgcctgac
     1561 aacgggacat gagtttgtat ttgtctagta aagactgggg cagattttct tctatcaaga
     1621 ggtccagtcc ctgatcaaaa gccgtcttga tgaccttaac cagactggcc tgactgattc
     1681 cctgagccag acgatagaca ggctggaggt catcttctac ctgagccaga accttcatcc
     1741 cagtcagact agccttagcg cggtcccatt ttccaaagac agcaagggtt gctcccaact
     1801 ctattttatc agccagatag ggctggttaa agaaattcac cgcaaaaacg acctctccct
     1861 gcttgagact aaaacgcagg cgattgcgct tgaaaccata atactggaca ctagcaggag
     1921 tcactacctg accagaaaga actgccttct caccgtcttc cagctccagc acctgcttgg
     1981 ttttgaagtc ttcataacgg aaaggaaagt agagcaagag atcttgcaag ttttcaattc
     2041 ctagtttggc gtatttttct gctgactttg gtcccacacc aggcaagaca tgcaagggtt
     2101 gatgtagatt catgctccac tcctttcttt tctaataata ttctctcgga atacggtcgc
     2161 tgaggaggca aaccacctca tagttaatgg ttacgcggta ggtcgctacc tgagttgcag
     2221 tgatttcctt atccccattg gagccaatca aggttacctt ggttccgagt gggtaaggct
     2281 taggcaatcg aatagtgatt tggtccatcg aaaccctgcc gacaattggg caagcttggc
     2341 catctaccaa gacagagaaa ttttgcatgt ctcttgtcca tccatctgca tacccgattg
     2401 gcacggtcgc gatgacttgc tcgctatccg cttgataagt tgctccatag cccatgcaag
     2461 ctccagctgg aactgtcttg acatgaacca gagcagactc caaggtcaag gccggtatca
     2521 aatcataagg caaatccaag accgctccac ttggattgag gccatacatg gcatctccca
     2581 tacgaaccgc attgaaaata gtctctacat gccaaagagt cgttgcagaa ttgctagcat
     2641 gaaccagctc tggaacttcc ttcatactag ctaaaatagt tttaaaccgt tctaactggg
     2701 cattaaaata gtcatctgat tcctcatcag cagtagcaaa gtgggtaaag attccttcaa
     2761 cacaaacacc gtgttgttgg agcaaatctt gagcctgctc aacctcactg gcctctctaa
     2821 aaccaatccg tcccatccct gaatcaatct tgaggtggac tgtcaatcca gttaggtcca
     2881 cttccttatc taagagtgct tgaatccact ccagtccagc cactgtcaag gtgaagtcat
     2941 attctttagc tagagcaaca gcttcgattt cagaaactcc taaaatgagg attggcttgc
     3001 tgagtccagc ttgtctgagt tcaatggctt catcgatatt ggaaacgcaa aagccatcaa
     3061 catcatcttg aattgccttg gcaacggcaa cagctccatg accataagca ttggccttaa
     3121 ccacagccaa cttgagcgtt ccttgaggga tatgagcccc catttgctga atattttgtc
     3181 gaatagctcc cagatgaatc agagccttgg ttggtctatg tggactagct ttcatgattt
     3241 tcctccaaaa tgacactggc tgtcacaaac tgatcggtgt ggctgataga cagccaaatc
     3301 tttcctgaaa atggtgcctg actaaaataa ggcgccccac gttcattgtt caagacttcc
     3361 aaatcctgaa aaccgagctt gctaatgccc gttcccatag ccttggaaaa ggcctcctta
     3421 gccgaccagc gaccagctaa atattctatt tgcctgcgtc ctttgagact ggtgaagcgc
     3481 tccatttcct gagcggtcag tacacgctta gcaaatcctt catgtcgtgt aactgcgctt
     3541 tctatcgaag ccaattcttc gatgtcaatt ccgtgtccaa ctatcattct catcaaaagg
     3601 agttgagatt ccccaactcc atccttttca cttattttgt caaggtaaca taaatctctt
     3661 ctaccaaggc ctctgtattt tcccaaccta ggcaagggtc agtaatagag caaccaaaga
     3721 tctctggttg gttttgacga ccatctgcta ggtaagattc aatcataaat cctcgaaccg
     3781 tctttttaat tttctcattc caatcacgat tctgcaaggt ctggcgaaca attcgaatct
     3841 gctccatata ttgcttgcct gagttatcat ggttggtgtc aatgaggata aaaggatttt
     3901 caagtcccat ggtttcatag cgttcaatgg cttggagtag gttttcatag tagtaattcg
     3961 gcatataatt gccatactcg ttgactgctc cacggaggat aacatgggcc aaaggattac
     4021 ctgatgtctc aacttcctgc ccatgataaa ggaaggtttg cttgttttga gcagcataga
     4081 tggcgttaaa cataacaccc aaatttcctg aggttggatt tttcatccct actggtgcat
     4141 caatcccaga agccacaaag cggtgctctt ggtcttccac agaacgagct ccaacggcat
     4201 ggtagctgac caagtcatcc accaagatca gatttgacgg ataaagcatc tcatctgccg
     4261 ttgtcaaacc agtctctgta atcacgcggt agtgcaactg gcgcacagcc tgcaagccat
     4321 taatcaggct tggagcctta gaagtatctg gctggtgaac taatccttta tagccgtctc
     4381 cattggtacg aggcttagca gtatacacgc gcatgaccat gaaaatctta tccgctacct
     4441 tcttttgcaa ggcggataaa cggcgagcat attccaagac cgcctcttca ttatcagaag
     4501 agcaaggacc aatcaccaag agaatacggt catcttcccc tgaaataata tctgccaatt
     4561 ccctgtcacg gctttccttg agtctcaagg cttccgcaga caattgggtt tcagccttga
     4621 tgacttccat atcgatttct tgaccttttt caataaatgc catcttattc tcctaacgtt
     4681 ttgtaaattt ctctgacaag agcttctgtg ttatcccaac ccaggcaagg gtctgtgata
     4741 gacttaccaa atacttctgg ctcattttgt cgaccatctt ccagataaga ctcaatcata
     4801 aaaccacgaa cgaactgctt aattttttca ttccaagcac ggttaatcaa ggtctggcgg
     4861 acaattcgga tctgttcaat atactgctta ccagaattgt catgattggt atcaatgatg
     4921 ataaaaggat tttccaagcc cattttctca tactgggcaa tggtatcaat taaattgtca
     4981 taatagtagt tgggaatatt ttttccatac tcattaagag caccacgaag aatagcgtgt
     5041 gaaagcgggt tcccagttgt ttctacttct tttcctaaga aaaggaaact ttgtttgttt
     5101 tgagcagcat aaatcccatt aaacatgaca ttgagatttc cagaggttgg atttttaaaa
     5161 ccagtagaaa atcctgcccc acttgccaca aagcggtgtt gctggtcttc aactgaacgg
     5221 gcaccaactg ccatgtaaga aatcaaatca tctacaagcg gaaggttttc aggataaagc
     5281 atttcatcag cagttgtcat ccctgtttct gtgatgacac gatagtgaag atggcgaacg
     5341 gctttgattc cattgataag actaggcgct tctgtcgcgt taggctggtg aatcaagccc
     5401 ttatagccat ctccgttggt acggggtttg gcagtataaa cacgcataac cataaagata
     5461 cgatctgcca cttcttcttg taggactgcc aaacgcttag cgtattcaag gacagcttct
     5521 tcgttgtcag atgagcatgg cccgattacc aagagaattc gctggtcttc tccacgtata
     5581 atggcttcta gctcttgatc tcgctgtgat ttcctctcca aagcctgacc ttctaatttt
     5641 gacaaggcac gaacttcttc tatatttatt ttagagcttt ttgctgtaaa taccataact
     5701 catctcctta taaagcaaac ggatacccag taaaaattta cttttcacaa gatatccgcc
     5761 tctaaactat ctcattttat tgtcttttac cgtgacagtt tttaaatttc ttaccagaac
     5821 cacatgggca aagttcattg cgtccaatct ggctcaaatc caaatcttct ggcatacttg
     5881 cttggtgagc agcgatattg cgagtcgctg ttgtactgat atgacgttct gcctgtggtc
     5941 tttcttgttc atgaatttgt gctttcatca tcaagcgtgt cacatcaaac tcaatcgaac
     6001 caatcatatc attaaacata cggaaacctt ctgcctgata ctcaacaaca gggttgttct
     6061 gagcatagcc acgaagtcca accgcgttac gcaattgatc aagggcatcg atatgatctg
     6121 tccacttgtt atccaccact cgtagaatca aaactttttg gaattcttta actgcttctt
     6181 catcgcgtag ttttgaaacc tgactatcgt aaaccttcaa ggcacgttgg aaaagctctt
     6241 ccttgatggc cttatcagac aagcctgaca agtcttccat cgtaatagaa tcttcaggaa
     6301 gcaagttgta cttagcaaag ttcaaaattg cctctagttt ttcatcttgt ttggcacgcg
     6361 catgaccatc aacgacacgt tcaatcgtgc gcttaatcat agactgaatt tcaggtgcca
     6421 agtcacgatc tgcagtgatg atatcgtaac gttgagcata gataatctca cgttgttcac
     6481 gcatgacatc atcgtattga aggacttgtt tacgggtatc gtagttattt ccttcgacac
     6541 gtttctgagc tgcttcaacc tgacgcgtca acatgcgaga ctcaatcgcc tcttcagaca
     6601 tgttcaagcg ttcaaagatt cccttcaagc gttcagaacc aaaacgtttc atcaaatcat
     6661 cttcaagaga taggtagaat tgtgactcac ctggatctcc ttgacgacct gaacgtccac
     6721 gaagctggtt atcgatacga cgactttcat gacgttctgt accaataaca caaagtcctc
     6781 caagttcacg aacaccttca ccaagcttga tgtcggtacc acgacccgcc atgttggttg
     6841 cgatggtaac ggcaccacgt tgaccagcat tcatgatgat ttgggcttct ctatagtggt
     6901 ttttggcatt caagacttcg tgaggaacac cagctgcgac caatttctta gaaatgtagt
     6961 cactagtttc aaccgctact gtaccaacca agacaggttg acccttttgg taacgagcct
     7021 taacgtcttc gacaaccgct ttaaacttag attcgatact tgcataaaga aggtctgagt
     7081 ggtcaatacg ttgaacagga cggtttgttg ggattggaat aacacgaatg ttgtagattt
     7141 cacgaaattc ttcttcctca gtcttacctg tacccgtcat accagacaat tttttgtaca
     7201 tacggaaaag gttttggtac gtgattgagg cagatgtctt ggtttcatcc tggattggca
     7261 caccttcttt ggcttcaata gcttggtgca atccatcaga ataacgacga ccttccatgg
     7321 tacgacctgt aaattggtcg acaatcaaga tttcttgctc ttcgctcacc acatagtcaa
     7381 tatcgagaag catgatgtag ttggcacgaa gggcgttatc gataaagtgg gtcaaagcca
     7441 cgttttcgat gtcatagagg ttttcaagtt tgaagtagct ttcagccctg tcaatccctg
     7501 aatcagacaa accaatagtc ttagactgca catcgatgat gtagtcatct ttgttcaaag
     7561 attttacata gtggtctgcc atgtgataca actgactggt ttcaaccgca ttggcacctg
     7621 atacaatcaa aggtgtacga gcctcgtcaa tcaagataga gtcaacctca tcgaccaagg
     7681 catagttaag cggacgttgt accatgtttt cagcgcgaac gaccatgttg tcacgaaggt
     7741 agtcaaatcc gatttctgag ttagttgagt aagtaatatc acactcatag gcttcttttt
     7801 tctccattgg agatttggta gccaagttaa tccctactga caaaccaagc caagagtaca
     7861 attcacccat ctcagtcgcg tcacgttctg acaggtattc attaaccgta actacgtgaa
     7921 cccctttacc tgaaagggca ttgaggtata ccggcatggt cgcagtcaag gttttccctt
     7981 cccctgtacg catctctggc acgtcaccat ggtgaagaac aatccccccc atgacctgaa
     8041 ccttataagg gaagagacct aggacacgtt tggcaccttc acggacaacc gcaaatgctt
     8101 cgtaaagcaa tgaatccagt gattctccat tttgataacg ttccttaaat tcaactgttt
     8161 ttgcttttag ttggtcatca gtcaaagcag ccatttggtc ttcgtatttg aaaaccttgt
     8221 cagccatctt ttccagacga cggatttctc ctttatcatt ttcgataatt gtttttaaaa
     8281 tattagccat gtttttcctt actttaaatt ccgaatattt tagaatgttc ctttaatttt
     8341 agcacaatta ctgattattt tcaaggaaga atccccattt tgaaaaggaa aaagaggtta
     8401 gttttcaagc taacctctcc gacttttatg actgtttaat tgccaaaaat tggcaaaatg
     8461 gcaaatgtga taaataagtt aatccaaaga gaaagacagc aaatcagtcc aaaaacaaag
     8521 agactgactt tcttggacag caaggccatc aaaatggaca gaattccaaa aactagcaag
     8581 actttctgta tgatgaagat gtcaatcctt acccccagaa tcgctctatc ccaagggata
     8641 aagaagaaag acagccagat cagcagaact aaaccaatat aaaccttgga atagcgtgta
     8701 ataaatgatt ttagatgtgc cataagccat ctcctaggat tgtatatcct ttacatttgt
     8761 atatgtccat tttcgacaac taatacatct gtacaaaggt ctgaaatatc ttccgatatg
     8821 tgcgacgtca ggataaccag accattttct tttttcaggt aagataaaat gacctgtttg
     8881 gttaacctca cactcttctc atccaaagca ttcataggtt catcgagaaa gagtatagaa
     8941 ggagaggaaa taaaggcttg aatcaaggcc attttttgct ttgttcccaa ggataaatga
     9001 cggtattcaa cgtcttcaaa ttcctgtaaa tcatagtatt gaatccaata ggcaattctt
     9061 ttttccgtaa ctttagatga aaaatacctt aacagttcca aattttctct cagggataaa
     9121 tgagataaaa actcgacttt ttcaattaaa attcctgcat cctgaatata atgatttttt
     9181 atcccgtaaa ctttcccgtc ttgaagaact tttcctttgt caaacttaat ataaccagca
     9241 agtatcttta aaagaaccgt cttgccagat ccattatcac ctttaagtcc ataaattttt
     9301 cctgattgaa acaccaaatt caactgattt aaaataggtc ttttcccata aattttctgt
     9361 ccatttataa tttcaagtct catcgtttat actttcttaa atttctcttc caagcgacta
     9421 acaatcaaca taactaataa gcttaagagt agtaaataca ctgttacaat atgatccatc
     9481 atccattctt gtcccacaaa tgctaaaaaa tagagaaaaa aggaaaaacc agcgcccgca
     9541 ctaatcagtg ctatcagata agacagtagt aagtacaaaa tcattaactg agcaagtaaa
     9601 aagaaaagtg cgactgtttt gaaatgaatt cctagcggaa gtaaaccaag aaaccagaca
     9661 aacaaatctt gagtaaaaaa tttagaaatg acaaccaatc gtttccttgt ataatcgaaa
     9721 aaggagcttg attgatagcg aatcaagtga tggtaatggt ctgccaaatc actttcaagt
     9781 aaaatgacag agagccatcc taccatgaaa agcgaaaggt ttaacaagag tttggggatt
     9841 tctaaattcg cttcagaata gtcattatag gaaaaaattg aaatcaattc tctcttgaaa
     9901 aagataacag catcctgctt ttgaaccgct gttgacaata gatggtttat caaaatcatc
     9961 atcagaacta tacgaagtag taaaacaatc tttctcttta gccaaatatt gttaaaattc
    10021 ttaaacatta tcttcatatt tttcaagcac catataaggg acaacataaa ggatataagg
    10081 agtaaagagt ttcatcaaac ttacatcccc atagctagct tgcatcaaac tcgtcatagg
    10141 aaccatatag taaggcaagg cactatagag cagcatagaa ccaagccaaa gaaacatcaa
    10201 ataggtgatt gccgaacgag tcacattccc cacataatca actatttgta aaaaatagat
    10261 tgcattgatg aaaataccga ttagtaggac acaagtaaag aacaaatagc tttttatctc
    10321 tccatctagg agtctttgta aaccacttcc atcagaaaat agagaattcc atccaagagg
    10381 agaaaaagtc ccaaagaaat aggttacaat tgcaattatc agcacagtca ctaaatatat
    10441 caaacaaggg atacttgcaa cttgcaaagt caactttctt tttaaccctt gataactcac
    10501 ttctcttcca atactcaatc gtaaaacttt atttttcagc tcaatatatt gaaaacctag
    10561 caaaacaatg atgattggaa taataaatga ttgataaaaa cctagattga actggagaat
    10621 acgaaaatct aatgctgatt ttaataagtc atttttaatt gcttgtccat cgaatttagc
    10681 tagatcgggc gcatgaatat caaaaaagaa ataagtcatc aaaacatcac gataaaccaa
    10741 agcaagaaaa agcaaagtaa gcaagacata aacaaattta tgccttgcca aagttaagag
    10801 aggaatcttc ataagccacc tcctacaaat agaaagtaat ataatactca atgaaaatca
    10861 aagagcaaac taggaaacta gccgcaagtt gctcaaagca ctgctttgag gttgtagata
    10921 agactgacga agtcagttac atatataatc caaggcgacg ttgacgtggt ttgaagagat
    10981 tttcgaagag tataaatcaa tgccttccgc ctttagactt ccctagttcc cgtctcaagt
    11041 caagcatttt tttgaaacag atatagtcta accagtcact agcaaaagcg ataagtagta
    11101 ataatagcca aataccccat aacttgatat ctgtcaaatt tctcaagacg atattgaaaa
    11161 acagaactga aaaaactgtc caagaaaggc taaaaagagc atagaggggg atgtaaatcc
    11221 agtaaaaata gtaaaaaatt gggaaaaatt tactttctct attatccttg tcaatccagc
    11281 tgaaaaagta aaaacagggt aataagatta tcattttaaa caagtgttcc atcacaagcc
    11341 tcctttttta aattgttttc tttagtttcg ttctattaaa tgttattacc aatataaacc
    11401 ttggaatagc gtgtaataaa tgattttaga ggtaccataa gccacctcct acaaatagaa
    11461 accgatataa atcaatgcct tccaccctta gacttcccta gttcctgtct caagcgaaac
    11521 atttctttga aacaggaata agttaaccaa ttcataccaa tagctagcag aataaaaaga
    11581 aaccaaatgc cccataactt gatatctgtc acatttctca agacggtatt gaaaaacaga
    11641 actgaaacaa ctgtccaagc aaggctaaaa agagaataga aggggatgta aatccagtaa
    11701 aaataataaa aaattggaaa aaacttacta tttctgttgg ccttttcaat ccagttatca
    11761 aaataaaagt acggtgctaa aagtaagaat ttaaacaaat gttccatcac cgacatcccc
    11821 ctccttcttt tgatagcgtt ttctattatt ttattatatc aaaagaatct ggaaatgtca
    11881 ttccagattc tactttttta tttacgcttt cttgcgatga gatgaatcgg tgttccctca
    11941 aagacaaagg ccttgcggat ttgattttcc aagaaacgta ggtaagaaaa gtgcatgagt
    12001 tcttcttcgt tgacaaagat aacaaaggtt ggtggtttgg ttgccacttg ggtcgcatag
    12061 aaaatcttga gacgttttcc tttgtctgtc ggtgttgggt tgatggcaat ggcatccatg
    12121 atgacatcgt tcaagacagc tgatggaata cgtgtatttt gactttcgct gatttgctta
    12181 atcatcccag gaagtttgtg gagacgttgc ttggttaaag ctgatacaaa gataatcggt
    12241 gcgtaaggca ggtattggaa ctgctcacgg atatcttctt cccagttttt catagtgtgg
    12301 ttatcttttt caagcgtatc ccacttgttg accacgataa tcatcccttt accagcttca
    12361 tgagcaaatc cagcgatacg cttgtcgtat tcacgaatcc cctcttccgc attgatgacc
    12421 attaagacca catctgaacg gtcaatagca cgcatggcac gcatgacaga gtacttctca
    12481 gtattttcat aaaccttacc agacttacgc ataccagccg tatcaatcat ggtaaactct
    12541 tgaccatctg tatctgtaaa gtgggtatca atggcatcac gagttgttcc agcaacagga
    12601 ctggcaatga cacggtcttc tcccaagata gcattaatca agcttgattt tccaacgtta
    12661 ggacgaccaa tcaagctaaa cttaatgaca tctggatttt cttcttcata ttcatttgga
    12721 aggttttcca caatcgcatc tagtacatcc cctgtaccga ttccatggac agatgagata
    12781 ggcagtggtt cacccaaacc gagagcatag aaatcataga tatcatttct catctcaggg
    12841 ttgtccacct tgttgactgc gaggataact ggtttgtggg tcttataaag cttacgagct
    12901 acgtattcgt ctgcatcagt aattccttcc ttaccagaca cgacaaaaac gataacatct
    12961 gcttcttcca tggcaatttc tgcctggtgc ttgatttgtt ccatgaaagg agcatcgaca
    13021 tcatcaattc ctcctgtatc aatcatacta aaagagcgat tgagccactc acccgttgca
    13081 tagatacggt cacgtgtcac tccttcgaca tcttctacaa tggagattcg ctcaccagcg
    13141 atccgattaa atagggttga tttcccaaca ttgggacgtc ctacaatggc aatagttggt
    13201 agggccataa tttctcactt tctacaataa cttcttctgt ttaagatttt ttctagttga
    13261 gcttggttct gcttggccaa actgttctgc tagacgctga ctccagcttg tggtcgcacg
    13321 cgctccagca tagtcagcct gaacacggtc ataagcttgg attgcctcag ttgactgttc
    13381 ttggtattct tcctcaaaga caacattctc tagtggcagt ctcggtttca tatcatgatg
    13441 ttgatttggc acacccagtg ccatcccaaa gacagaatag gtgtagtcag gtaggttaaa
    13501 gagctctgcc acttcttcag acttgtatcg aaccaaaccg ataatcacac caccatagcc
    13561 caagctttca gctgccaaca aggcgttttg tccagcaaga gctgcatcga ccgaactaat
    13621 caagagacct tccacacctt ggggttggaa ggtgtcggta tgaagtcggg ctcccttttc
    13681 tgctcggttc aaatctccga caaagagaag gaaaacagca gactggcgaa tggcttcttg
    13741 aggtaccaat tcatacaagg catctttctt ctcttgactt cgtaccacaa tcacagagta
    13801 ggattggaaa ttcttccaag atgatgccat ctgggctgct gtcaaaatct catttaagtc
    13861 tacttgggga atttcttgct ctttaaacct gcgcactgaa gtatgagcct tcatcaattt
    13921 aatggtttct gtcatcgacg gtttactcct tctaaacgag tctcctcagc caaataacgg
    13981 atgcgttcca tgacacgtct ggcttcccag gtttcgtcat ttccattttt ccctttagcg
    14041 aaatgcttct ccaaatcttc aaagttgaag ttagaggtga aaaaggtcgg taaattttcc
    14101 tgcatccgat attggagaat gacctgcagg atttcatcac gcacccaagc ggtggattgc
    14161 tcggcaccaa tatcatctaa aatcaggact tcagaaagct taatctcatc cactaaggtc
    14221 ttaacattgc catcaccgat agcatttttg acatcaatga caaagctagg atagtggagg
    14281 agagtggatg atacaccacg tttttctgat aaatcatgag ctagggcagc caccatgaaa
    14341 cttttaccca caccaaagtc tccatataag taaagacctt tcaaattatt ggggtaatcg
    14401 ttaacaaatt ctatcattct ttttagaaca gttaagcgct gaacatcatc acgataaaca
    14461 tctaaaaaac tgacattttt caggctagat ggaaaattaa ttaagttgag acgtttcttg
    14521 atagccgctt ctttttcagc tgcgattagt tcaggagttt cttcataaga aacatccgca
    14581 tagccatggt tcatgactaa tataggcttg tagcctttgg caatataatc tgtatctcct
    14641 cggaggaatt tatctctctc agtgatgtat tggttaaact tggaaatact acggttcaac
    14701 tcgtcctgat tgagggattc ttgctggaca aaggccgcaa catcaggatc cttcatgatt
    14761 ttctgaacta aatcttgata gtgaaaacga cttggttgac gtttgagtac atctccgaca
    14821 ctttccatct aatcacctcc gttatctaat tttgctaata attctagggt ttggcgttcc
    14881 atctcttcta attcctcagc ggatgacgta tttttatatt caggattact ccatttagga
    14941 atattggttt ttgctggggc agtctgattc tgtttttgtg tttttgtttt ctgccctcgc
    15001 tcacggatgc gcaagactgc cttttctgcc gaatgaatct tttgataggc atagtcattg
    15061 gctaccttca tggcatattt ctcattgata tttgccgaat cgaccttatt aaaggtcaat
    15121 aaaagaatga tattgatgac ttcgtccagc aagcccaagc cagccatctg ttgcaagagt
    15181 tctctttctg tttgggtaat ggttcccttg cgtgtttgct tgatttctgc caagaactgc
    15241 agggcagttt tacttttggc ttctttgata atggttgttt ccttaagact aaagtcagag
    15301 gaaactggtt tttgagcaat tttttcacgc atgcgtttgg ttgaaataac ctgagaaaca
    15361 gctgttgact tggccaattg ataggtttca aaccaagtcc atttcttctc ctcggcaata
    15421 gcaaagaggt ttaagacatc ggactgctca tccgcaaaac gaagtccatc tcgagccatc
    15481 agctggcgaa aatgttccaa gtcaaaatca ttggccactt tcttcttgag accaaggtct
    15541 tcttgactgc ctagttctgc caattctgga aagacttgat tgagtgagac aggtatttct
    15601 tcaccatcag cactttcaac tttcaaatcc tccacagcag agtcgccaat ctttttctct
    15661 aagagtctgc gataaacagg atgctccaag aagtcttgac tagatagagg agcatggagg
    15721 gctagctgat aaacatcccc cttttgatag agggtcaaga gattaaaagc agataagatt
    15781 ttcaatgatt ttatcagtct atccatccca aagttgagat gattgaggat acttgaaaaa
    15841 agatattcct ttctaccatt atcccaaaaa ctgattgtat aaagataaag gctcagtgcc
    15901 tcctgaccga taatcgggag gtagcactgt accagagatg aggtatcttg cgacacccga
    15961 ttattcttta gataagaaaa acggtcaatt ggcttcattt atctttcctt tttcttttta
    16021 gaggactggg tgatttgttg gagcaagctc tctaactcac tgacatcctt aaaactacga
    16081 tagacactag caaaacgtac ataggtaatc tcgtccaatt cagccaactc ctccatgaca
    16141 agtgaaccaa tgtcctcact ttgaatttca ttttcatttc gaccacggag tttctgttcg
    16201 atacgattga ctaccatgtt gatttcatca cttgacacag gacgtttctg ggctgagcgg
    16261 ataatcccat taaagatttt atctctggag aattgttccc gtgtgccatc ttttttaaca
    16321 accactaagg ttctttcttc tactcgttcg taggttgtaa aacggtgttg gcactcgtcg
    16381 cactctcgtc ttctacgaat ggtgttccct tcttctgctt ggcgactatc gataacactt
    16441 gacttggtag ccccacattt tggacaacgc atcctttccc tccttatcgt tttcttttca
    16501 ttataccatt ttttaagcga ttcccaaaac aattcttctt tttgcttgac aagttttttg
    16561 ttttgttgta ttatttaatt aagacaaaag gataagagaa aggagactaa gatgtcctgg
    16621 acatttgaca acaaaaaacc catctattta cagattatgg agaaaatcaa gcttcagatt
    16681 gtttcccata cactggaacc caatcaacaa cttccaaccg tgagggagct agctagcgag
    16741 gctggtgtca atcccaatac catccaaaga gccttatcag accttgaacg agaaggattt
    16801 gtctacagca agcgaacaac tggacgattt gtgactaagg ataaggagct aatcgctcag
    16861 tcacgcaaac aattatcaga agaagaattg gaacacttcg tttcctccat gacccatttt
    16921 ggctatgaaa aagaagaact accaggcgta gtcagtgatt atattaaagg agtttaagcc
    16981 tatgtcatta ctagcatttg aaaatgtatc caaatcatat ggagcaacac cagcccttga
    17041 aaatgtttct cttgacattc cagctggaaa aattgtcggc cttcttgggc caaacggctc
    17101 aggaaaaaca accctgatta aactaattaa tggcctctta caaccagatc aaggacgtgt
    17161 cctcatcaac gacatggacc caagcccagc aaccaaggcc gttgtagctt atttgcctga
    17221 tacgacctat ctcaatgagc aaatgaaggt caaagaagcc ctaacctact tcaagacctt
    17281 ctataaagat ttcaatcttg aacgcgctca tcatctactt gcagacctgg gcattgatga
    17341 aaatagtcgt ctcaagaaac tatcaaaagg aaacaaagaa aaggttcaac tgattttggt
    17401 tatgagccgt gatgctcgtc tctatgtttt ggatgaaccc attggtgggg tggatccagc
    17461 agcccgtgct tatatcctca ataccattat caacaactac tcaccaactt ctaccgtttt
    17521 gatttctacc cacttgattt ctgatatcga gccaatcttg gatgaaattg tcttcctaaa
    17581 agacggaaaa gtcgtccgtc aaggaaatgt agatgatatt cgctacgagt caggtgaatc
    17641 cattgaccaa ctcttccgtc aggaatttaa ggcctaagca aaggagatta tttatgtttt
    17701 ggaatttagt tcactacgaa tttaaaaatg ttaacaagtg gtatttagcc ctctacgcag
    17761 ccgtgctagt cctttctgcc ctcatcggaa tacagacaca aggctttaaa aatctacctt
    17821 accaagaaag tcaggctact atgctacttt ttctagctac agtctttggt ggcttgatgc
    17881 ttacacttgc gatttcaacc attttcttga ttattaaacg cttcaaaggt agtgtctacg
    17941 accgacaagg ctatctgact ttgaccttgc cagtttctga acaccatatc atcacagcca
    18001 aactaatcgg tgcctttatc tggtcattga ttagcaccgc tgtattggct ctaagtgctg
    18061 ttattattct ggctttaaca gctccagaat ggattcctct ttcttatgtg attacatttg
    18121 tagaaacaca tctccctcag atctttctta caggtatatc cttcctacta aataccattt
    18181 caggaatcct ctgcatctac ctggctattt ccattggaca gcttttcaat gaataccgta
    18241 cagcactcgc tgttgcagcc tacattggta tccaaatcgt cattggattt attgaacttt
    18301 tcttcaatct tagttccaat ttctatgtca attcactggt aggactcaat gaccatttct
    18361 atatgggagc aggtatagcc attgttaaag aactcatatt catagctatc ttttatctcg
    18421 gaacctacta catcttgaga aataaggtta atttgcttta aataattttt acctagatat
    18481 gtaacatact catagaacaa aagagaccag gcaaaaagtc tttaaaatta gaaaacgcat
    18541 agtatcaagt gttaaaaatc cttgatatta tgcgtttttt gtgagctgac ttatttcctt
    18601 tcgctatatc gcaaaatgaa ataagaacgg aacgatggga ttttggaatt caaatcaatt
    18661 tataagaatg ttttagaagt aatattatcc tattccagat tcagttcact atacaattga
    18721 gttttcaagc aacctgttta cataatgtgt acataattag gttcgtgatt ccaccctttt
    18781 cacctttaaa aacctcgctt tcgcaaggct cttctattta taagataagg cacgtttaaa
    18841 ggttttccaa atccctaaat catccgtttg aagaacgaga ctagcataca tgcgtccgat
    18901 aaatcctgtt gctaccaccg caaaaatcac tgtaatagca agtgaaatcc atgcttccgc
    18961 tccctccgca tagccattaa tcgttcgaaa cggcataaag aaggtcgaaa taaagggaat
    19021 ataagaacca atcttcaaga ggagattgtc accagctgca cctagagctg tcactccaaa
    19081 aaaaccaccc ataatcaaaa tcatcaaagg cgacaaggct ttccctgagt cctcaggacg
    19141 agaaaccata gatcctagga aggctgccaa gactacgtac atgaaaagac tgatcaaaat
    19201 aaagagcagg gtattcagtg agatagcatc tcccaagtga tccaaaatac cagactgagc
    19261 caagaatggc aaatctttaa agagcaaaac ggcagccaga ccacctacaa catagatccc
    19321 aatatgcgtt aaaatcacta gaaacagagc catcatccgc gcatagaaat agtgacttgc
    19381 ccttatgcta gaaaagacga cttccataat tttggtgcct ttttcactgg caacttcctg
    19441 agctgttaca cccgcatagg taatcagaat catataaaga aagaatccta aggcacctgc
    19501 tgcaattgtt tgaataaact ttttattttc cttggcttca tcaatctttt ctgtgaattg
    19561 aattgtctgc gctaagcgtt tttcctgctc ttgagacaag gaagcagttg aacgattaag
    19621 ctgattttgc agttcattga gtgtacctgt aacctcaaat ttaattccat tttcaagcga
    19681 tgtttcgcca tgataaactg cctttagaac actatcttct tgatcaatgg tcaaataacc
    19741 ttttaatttt tcttctttaa ttgcttcttt ggcacttgct tcatctttat agtcgaagtt
    19801 aacaccattt acattcttca gtccttctgc tacagatggc actgttgtca ctactgccac
    19861 tttattattt ttagccatag aagaaccttg gagatgccca attcctacag agattcctaa
    19921 aaagaggaac ggcgaaatca ccataaagaa gaaactccat gactcgacat gtcgaagata
    19981 ggtttccttg attacaaccc acatatttct catacttcca ctcctgattc tagtttaaag
    20041 atttcatcga tagttggcgc ttgttggtca aatgttgcga tatattgacc ttgagtcaag
    20101 attgggaaga gttcccttcc agcgctctca tcctccaaaa tcaatttcca actgccttgt
    20161 ttggtcaagc tcacctgttt gacatgagga agattttcca attcttcctt gcttcgttca
    20221 cttgaaacaa agagacgcgt tttcccgtat tgattgcgga catcctgaac tggtccgtgc
    20281 aagaccacac ggccatctcg gatcatcaga atatcgtcac aaagttcctc aacattggtc
    20341 atgacatggt cagaaaagat aatggttgct ccgcgctctt tttcctgaaa aatgacttgt
    20401 ttgagcaatt ctgtattaac tgggtccaat ccactaaaag gctcatccaa gataatcagg
    20461 tctggttcat gaatcagagt aataatgagc tgaatcttct gctgatttcc ttttgacaga
    20521 ctcttgattt tatctgtcag ctttcctttc acttccaacc tcttcatcca ttgagggagt
    20581 ttttctttga cttctttggc atccatgcct tttagagtcg ccaagtagcg aacttgttca
    20641 agaactgtca atttaggcat gagactgcgt tcttcaggca gataaccaat ccgagcatag
    20701 gtctcctgac gaatatcctg accatccaga ccgatttctc cctgatattc taggaatttc
    20761 aaaatactat ggaaaatcgt tgtttttcca gcaccatttt ttccgactag tcccaaaata
    20821 cgacctggtc gcgcttgaaa gtcaatacca aacaaaactt gcttggagcc aaaacttttc
    20881 tctagacttc ttacttctag catctttcac ctccgaaatt tcttgcactc attatactcc
    20941 tttttgatag cctttacaat gtttttgtcc atttttagaa gactattgct gtgtaaaata
    21001 tggcctggag cacttttata ctcaatgaaa agcaaagagc aaactaggaa gctagccgta
    21061 ggctgctcaa agtacagctt tgaggttgca gataaaactg acgaagtcag ctcaaaacac
    21121 tgttttgagg ttgtggatag aactgacgaa gtcagtaact atatctacgg caaggcgaag
    21181 ctgacgtggt ttgaagagat tttcgaagag tattagtgat aaatccatta tacagtagca
    21241 aacttaattt ataccttccg ctcctcaact gtctattttt aatcctgaat tgttatttga
    21301 gtaactcctt tttcctcata aagttttctt cctctaaaac ttctggaaaa aagcgaatag
    21361 tttcagacaa catttttata agaaacaagt tcatctgtca tttcaagaag gagtaatcct
    21421 ttatctacta atggacggaa cggaattcaa ccgcttgtcc gatatgtttt ctaaggatta
    21481 tatagcaaaa tgaaataaaa acaggacaaa tagatcggga cagtcaaatc gatttctaac
    21541 aatgttttag aagtagatgc gtactattct agtttcaatc tattatatct attatgcaca
    21601 ccactatagg atctaataaa aatcacaaca agttcattca tagatggtta cctcagggaa
    21661 ctaagaaaac gactacaaag gaagtcgcat tcatcgaaaa gtggattaac aactatccta
    21721 aaaaatgctt gaactacaag tcccccagag aagacttctg gatgactaac ttgaacttga
    21781 aatttagcaa taattaattc accatctaac tatatttagt aattatttca gaactgatta
    21841 atattaaaat taactaacaa ttcaaaggat tcatactagc cataaattac gtccatcaga
    21901 gagagactct tactactttt agattttagt ctttctagct tcagaataca tctaaacttt
    21961 agggaaaatg actattcgaa agcgcgaatg cctcaaaatt atctcagata agctattcga
    22021 aacttagaat gcttttaaat tcatggaatt gcgattattc gaaacctaga atgcatataa
    22081 cctttagttg acagacctat tctaagtctc gaagggctat ttactttcta ttccttatca
    22141 aaaaagactc attccccctt tctcctccaa aatatggtat agtagaaata tactatctat
    22201 gaggagttta catgtcacag gataaacaaa tgaaagctgt ttctcccctt ctgcagcgag
    22261 ttatcaatat ctcatcgatt gtcggtgggg ttgggagttt gattttctgt atttgggctt
    22321 atcaggctgg gattttacaa tccaaggaaa ccctctctgc ctttatccag caggcaggca
    22381 tctggggtcc acctctcttt atctttttac agattttaca gactgtcgtc cctatcattc
    22441 caggggcctt gacctcggtg gctggggtct ttatctacgg gcacatcatc gggactatct
    22501 acaactatat cggcatcgtg attggctgtg ccattatctt ttatctagtg cgcctatacg
    22561 gagctgcctt tgtccagtct gtcgtcagca agcgcaccta cgacaagtac atcggctggc
    22621 tagataaggg caatcgtttt gaccgcttct ttatttttat gatgatttgg cccattagcc
    22681 cagctgactt tctctgtatg ctggctgccc tgaccaagat gagtttcaag cgctacatga
    22741 ccatcatcat tctgaccaaa ccctttaccc tcgtggttta tacctacggt ttgacctata
    22801 ttattgactt tttctggcaa atgctttgac acgtaaaaaa tccgtttggt ttcccaagtg
    22861 gattttgtgc tttattttga aacttctttt gcaagaacaa agttcccaag tgtggcagaa
    22921 ccatttcctg cgactgctgg cgtcacgata tagtcacgca catctggtac tggtaggtaa
    22981 ccattaagaa gagatgtaaa tttctcacgg acacggtcca gcatatgttg ttgagccatg
    23041 acccctccac caaagacaat cacgtctggg cggaaagtca ctgtcgcatt aaccgcagct
    23101 tgagcgatat agtaggcttg aacatcccaa acagggttgt tgagttcaat agtttcccca
    23161 cgtacacctg tacgagcttc caaacttgga ccagctgcat aaccttctag acatccctta
    23221 tggaaaggac aaacaccctt aaactctttt tcaatatcca ttgggtgtct agcaacataa
    23281 taatgaccca tttcagggtg acccacacca ccgataaact caccacgttg gatgacgcct
    23341 gcaccgatac ctgtaccgat tgtgtagtaa accaagtttt cgatacgacc accagcattg
    23401 ttacgggcaa ccatttcacc gtaagcagag ctgtttacgt ctgttgtgaa gtacattggc
    23461 acgtttaggg cgcgacgaag ggcaccaagc aagtctacat ttgcccagtt tggttttgga
    23521 gtcgtcgtga taaagccata agtttttgag tttttgtcaa tatcaatcgg cccaaatgaa
    23581 ccaactgcaa gaccagcaag gttatcgaat tttgagaaga actcaatggt tttatcgatt
    23641 gtttcgattg gagttgttgt tggaaattgt gttttttcta caacgttaaa gttttcatca
    23701 ccgacagcac agacaaactt tgtaccgccc gcttccaagc ttccatataa ttttgtcatg
    23761 ataaacctct tgtttttatt ttctttatta tagcatactt cgaaagtcta aatgtctcta
    23821 ttttttagat tttcctctgt aaatcttact atctaataaa aacgaacaaa catgtcattt
    23881 gttcgttttc acattagaga ggattgatta gattttcact tcgatcacag catccccctt
    23941 cgaaactgaa cctgttgcga ctggagctac tgaagcgtag tcagctgtat ttgtaacgat
    24001 aaccattgtt gtatcatcaa gtccagctgc agcgattttg tttgagtcaa atgttccaag
    24061 aacatcgcca gctttcacct tattaccttg agcaactttt gcttcaaaac cgtcaccgtt
    24121 catagataca gtgtcaatac caacgtgaat caaaacttca gcaccatttc ttgttttcaa
    24181 accaaaagcg tgccctgttg gaaaggcaat tgaaacttca gcatcagcta gtgcatagac
    24241 cacgccttga cttggtttca cagcgatacc ttgtcccata gctccacttg agaagactgg
    24301 gtcattgaca tcagcaagag cgacaacatc accgacgata ggagttacaa gtgtttcatt
    24361 ttgaagagct gctggcgcaa cttcttcttt ttcttcagcc acttcagctt gttttgcagc
    24421 tgcagttgcg tctacttcat cttcgtaacc aaacatgtaa gtaagagcaa aaccaagggc
    24481 aaatgataca gctaccataa gaaggtattg tggaagttgt ccgttaccaa cataaagcat
    24541 tgtaccaggg atgatggtga taccattacc agtaccagca agtccaagga tagaagccaa
    24601 tccaccaccg attgcaccag caatcaatga aaggaagaat ggtttacgga agcgcaagtt
    24661 caccccgaag atagcaggct ctgtaatacc taggaaggca gaaagagcag ccgggaaagc
    24721 aagtgttttc agttttggat tttttgtttt aacaccaacc gcaacagtag cagcaccttg
    24781 agctgtcata gcagctgtga tgatagcgtt gaatgggtta gcatggtcag cagcaagtaa
    24841 ttgcacttca agcaagttga agatgtggtg cacacctgac acgacgatca attggtgaac
    24901 cccaccaatc aagaaaccac caagaccaaa tggcatgcta agaatcgctt ttgtagcaat
    24961 aaggatgtag ttttcaacaa cgtggaaaac tggtccaata acaaagagtc caaggataga
    25021 catgaccaaa agtgtcacga atggtgttac caagaggtca atgacatctg gaacaacctt
    25081 gcggacagct ttttcaaatt tagctccgac aaccccgatg atgaaggctg gaagaacgga
    25141 accttgcaaa ccaacaacag ggatgaaacc aaagaagttc atcgctgtta cttcaccacc
    25201 ttgagcaact gcccaagcgt ttggaagtga gccagagaca agcatcatac caagaacgat
    25261 accaacggca ggatttccac caaatacacg gaaggttgac cacacaacca aacctggcaa
    25321 gatgatgaag gctgtatctg tcaagatttg tgtgtaagtt gcaaagtcac ctggaagtgg
    25381 catttcaaga gcgttgaaaa gaccacgcac acccatgaag agacctgtcg ctacgataac
    25441 tgggatgatt ggaacgaaaa catcaccgaa agtacggata gcacgttgga accagttccc
    25501 ttgtttagca acttctgctt tcatgtcatc cttagatgat gttggtaagc caagtacaac
    25561 aacttcatcg tacattttgt taactgtacc tgtaccaaag ataatttggt attgccctga
    25621 gttaaagaaa gcaccttgaa ctttttccaa gttctcaatc acttctttat tgattttctc
    25681 ttcatctttg accatgacac gtagacgagt cgcacagtgg gcaacactat tgacattttc
    25741 acgtccgccc aaggcatcga tgactttttt tgcaatttcc tgattgttca tttgcaaaaa
    25801 tctccttata taacattttg ttcttgtttg aaagcgattt tattcgccct tacgactatt
    25861 attttatcat gttttaaaaa tatgtcaagc gttttgcaga aaaaatattc tatccactta
    25921 gtgggcaagt tttagattga ttgtttttga ctgtttcatc aggaattcct tgaaaaataa
    25981 gtttgaaacc ttggttaaaa agtattttat ttcattatct ttcacatttt cgtaaaaatt
    26041 tgattgattt cctgtattta tttcatgata atcgtttgac agttttttct attttttaat
    26101 ataaaaggct tgcatttttt atcgaaaaca gttactatta aaagtgataa gatttttgga
    26161 ggaatgaatg gaatggacaa ctgagcgtcg ttacagactt tatcaagatt ggacgcaaga
    26221 agaaattcaa catataaagg aaaatatggc acaatctcca tggcatactc attaccatgt
    26281 tgagccaaaa acaggacttc tcaacgaccc aaatggcttt tcttactttg atggcaagtg
    26341 gatcctcttt taccagaatt ttccttttgg tgcagcccac ggtttaaaat cttgggcaca
    26401 gctagaaagt gatgatttgg ttcactttag agaaactgga gtcaaagttt tgccagatac
    26461 tccattagat agtcacggtg cctactctgg ttctgccatg caatttggcg ataacttatt
    26521 cctattttat acaggaaatg ttcgcgatga aaactggatc cgtcacccat accaaatcgg
    26581 tgctttgatg gacaaggagg gtaagattac aaagattgac aagatcttga ttgaccagcc
    26641 agcagactct actgaccact tccgcgatcc acaaattttt aactttcagg gtcaatatta
    26701 tgccattgtc ggcggacaag acttggagaa aaaaggtttc gttcgtctct acaaggctgt
    26761 caataacgac tacacaaact ggcaagcagt tggcaacctt gactttgcta acgaccgtac
    26821 tgcctacatg atggaatgtc ctaatctggt ctttgtagag gaacagcctg tccttctcta
    26881 ctgtccacaa ggattggata agaaagttct agactacgat aatatctttc caaatatgta
    26941 taagattggg gcttcctttg accctaaaaa tgccaaaatg gtagatgtgt ctcaacttca
    27001 aaacatggat tacggtttcg aagcctatgc aactcaagcc ttcaacgctc ctgatgggcg
    27061 tgctctagca gttagctggc ttggtttgcc agatgtttct tacccatctg accgttttga
    27121 ccaccaagga accttctctt tggtcaagga actcactatc aaagacgaca agctctacca
    27181 gtatccagtc gctgctatta aggaccttcg tgcttctgaa gaagccttct caaaccgttc
    27241 ccaaaccaag aacacttacg aacttgaact caacttggaa gccaatagcc agagcgagat
    27301 tgtcttactt gctgataaag aaggcaaggg actttcaatc aactttgacc ttgtaaacgg
    27361 tcaagtgaca gtggatcgta gccatgctgg agaacagtat gcccaagaat ttgggacaac
    27421 tcgttcttgc cctatcgaga atcaggctac tactgctaca atcttcatcg ataactctgt
    27481 ctttgaaatt ttcatcaata aaggagaaaa agtattttct ggtcgtgtct tcccacatgc
    27541 ggaccaaaat ggtatcctga tcaaatctgg aaacccaact ggaacttact atgaattaga
    27601 ttatggtcgc aaaactaact gatgtcgcca aacttgcagg cgtcagtcct actaccgttt
    27661 ctcgggttat caataaaaaa ggatatctat ctgagaaaac catccaaaaa gtcaatgaag
    27721 ccatgagaga attaggctat aaacccaaca acctggctcg tagtctgcaa ggaaaatcag
    27781 ctaagttaat cggcttgatt ttccccaata tttccaatgt tttctatgca gaattgattg
    27841 ataaattgga acaccaactc ttcaaaaatg gttacaagac catcatctgc aacagtgaac
    27901 atgattctga gaaggaacgt gaatacatcg aaatgttgga agccaatcag gtggacggca
    27961 tcatttctgg tagtcacaac ctaggaatcg aagactacaa tcgtgtgaca gcgccgatta
    28021 tttcctttga ccgaaaccta tcgccagaca tccctgtcgt ctcctctgac aactatgctg
    28081 gtggggttct tgctgcccaa accttggtca agacaggtgc ccagtctatc atcatgatta
    28141 cagggaatga caattctaat tcgccaaccg gactgcgcca cgctggtttt gcatccgtac
    28201 tcccaaaagc tcctattatc aatgtttcca gtgacttttc tcccgtcaga aaagaaatgg
    28261 aaatcaagaa tatcttgacc cgggaaaaac cagatgccat ttttgcttcg gatgatttga
    28321 cagctattct ggtcattaaa atcgctcaag aattgggcat ttctgtccca aaagagctca
    28381 aggtcatcgg ctatgatggg acctacttta tcgaaaatta ctaccctcaa ttggctacta
    28441 tcaagcaacc tttggaagag attgcttgtc tcactattga tcttctcttg caaaagattg
    28501 aaggcaagga agtcgccaca actggttact tcttaccagt tactctatta ccaggaaaaa
    28561 gtatttaaac acaagaaaac tcagaccgat tcgtctgagt ttttatgatc ttaaattttc
    28621 gagatagcgc tgggctgtct ctaggttaaa ggttttatct gcgataagct gctcgactag
    28681 gggagcaact tcagattcac tagccccagc taggagagct agggatttgg cctgtagttt
    28741 catgtggcct tgctggatgc ccgtacttac caaggctttg agggctgcaa aattttgagc
    28801 aagaccgatg gacacgataa tctgggctaa ttctctggca gaaggatttc ctagtagatc
    28861 atgactgaga actacacgtg ggttgaggcc gatagagcca cccttagtcg ctacaggcat
    28921 gggcagggtc atctcaccga ccaattcttc tctttcaagg tccagcgtcc agcaactaag
    28981 accttgataa cgtccatctc gactggcaaa ggcatgggcc ccagcttcga tggcacgcca
    29041 gtcattacca gtggcaatca aaatcgcatc aataccatta aaaattcctt tattatgagt
    29101 agcagctcgg taaggatcag cctgcgcaaa ctgactagcc aacgcaattt tctccgcaat
    29161 ctctcgtcct tgatcctttt ggcggctcaa gtagcgaaag gcgatgcgac agcttgcagt
    29221 caccagagaa tcggtcgcgt agttggacag gattgccatg agactctgtc cctgactgag
    29281 ttcttctaag actggtttca aggcttccag catggtgttg agcatattgg cacccatggc
    29341 ttcctgggta tcgacatgaa tataaacaac gagaaagtct ggttcacctt ttatctgctc
    29401 gacatgcaga tcacgcgccc cacctccacg tttaacgata gaaggatagg cttgattggc
    29461 aagctccaag agctccgctt tcttgctggc aatcttctct tgcgctagtt taggattagc
    29521 aacttgataa agggctacct gcccaatcat ctgtcgctga tggacttgtg cagtaaaacc
    29581 acctgcacgc ttgatgattt tgctggcata gctggccgcc gcaaccacag agggttcttc
    29641 tgtcacatag ggaacggtgt attcctgacc gttgacaagt acctccggaa ccagtgaata
    29701 aggcagagaa aaagttccca ctacattctc actcagctgg tctgccacag tcacgctcat
    29761 ctgttcatcc ttctccagac tagcttgtct ctcaggacta aggagcgcct gagcttttaa
    29821 cagctcgagg cgctcttggt atgatttttt agaaaatcca ttccaactta tcttcattat
    29881 ttttcaacct tgctataacg gcgttggtgg tcgagaattt caaccaaggc aaaatcttga
    29941 ttttcatagc cagcaaactg ggcagagttt gtttcatcca agtttacttc ctcaaaaaag
    30001 accttttcat agtctgcaac ggatagggca gttcgttggt tgagcttgtt caagcggtct
    30061 ttatccaaat aagcttcata tccttcaacc aattcaccac tgaagaactc agccacagct
    30121 ccacttccgt aactataaag ggcgatttta tccccagctt tcaagctatc tgtattttcc
    30181 aagagagaca aaagtccaag gaaaagtgaa cctgtgtaga tattccccac cttttgactg
    30241 tagagaatag actggtcaaa atgcttttgt aagaggtctt ttttctcttg aggcaggctc
    30301 ttatccatga tttttttcaa gccttttagc gctaatttag gataaggcaa gtggaaacaa
    30361 acagccgcaa aatcatccaa agtaagctgg tagcgttttt gatattcaag ccaagtcgtt
    30421 ttcaaactat ccaagtattg ttgggtagaa tagacaccat ttacataagg agttgtcgag
    30481 taatttggtc gccagaaatc catgatgtca cgggtctgag ctacattgtc attattaaag
    30541 gccatcatgc gtggattttg tgtaatcaac atagctacac ttccagcacc ttgagttggt
    30601 tctcctggag tttcaatacc gtatttggca atatcactgg caatgaccaa gaccttggac
    30661 tccggagaat tttccacatg caatttggca taatggaggg cagcagtcgc tccgtagcag
    30721 gcttctttaa tctcgaaact acgagcaaag ggctggatgc ccagcaagcc atgcacaaag
    30781 acggccgcag ccttactctg gtcaattcct gactcggtcg ccacaatgac catgtcaact
    30841 tcttgtcttt cttgctcagt taaaatagag tcactagcac tggccgccaa ggtcacgata
    30901 tcctcagtta ggggcgcaat actcaattcc ttgagtaaga gtcctttact taatttttca
    30961 gggtcaattc ccctcgcttc tgctaagtct tgtaatttca agacatattg actggtcgca
    31021 aaaccaatct tatcaatacc gattgtcata tttacctctg ttttatcatt catgtaaaaa
    31081 atcgttctat actattttat cacaaatggc agtaaaagag agaaaaaaga cttgattcac
    31141 caaatcaagc ctcttattgg tcatcatttt aaagaatgat tagttgctag agagttcacc
    31201 gatataagta gctttataag ctccattcac agttatcagc tcctggagga tcaaatttcc
    31261 tgagtaagtc cttcccatct catctacaaa tttttgataa aactgactgg tcggaatttc
    31321 tctgacatcc ttatcaaatg tcttatcaag tgttttacta accttctcag caatcaattg
    31381 atgctcttgc catccacttt gaaactctga gcccgaacta gaaaccatga ctgggataaa
    31441 caacaaggtc agtagactta cagacaataa ggaaaatagt agacttcctg caaaactaga
    31501 atcctagttc atgattgata ataacagcaa tcaaattcat tcgtaatccg aagcgtttac
    31561 gatgatttcg ataggttgtt gaaaacattt taaacgtttt tactttggca aagatgttct
    31621 caaccttgct tctctcctta gatagcgcat ggttataagc tttatcttca gctattaacg
    31681 gcttgaggtt gctggattta cgtggagttt gtgcttgagg atatatcttc atgggccctt
    31741 gataaccact gtcagccaag attttaccag cttgtccgat atttctgcga ctcattttga
    31801 acaacttcat atcatggcaa tagaaacaat tctcccttga cttgtgacaa tcgcttgagc
    31861 cttcatagcg tggcattttt tttaccagaa tcattcgcta attctttttt tagggcgatt
    31921 gatttgtact tccgtcgcat caatcattac cgtgtcctca gaactgagag gagttcttga
    31981 aatcgtaaca ccactttgaa caagagttac ttcaacccat tggctccgac ggattaagtt
    32041 gctttcgtgg ataccaaaat cagccgcaat ttcttcataa gtgcggtatt ctcgcacata
    32101 tagaaagcgt tgttaattta tttatctcat ttttcagaaa attcttttat ttctgtaaag
    32161 tctacgatac tcgatgtgtc tttatataat tatacagtct gagaatcact gttccgctag
    32221 ccattccaat agagattacc agagccaaca tgacaaccaa ggtcgcactt gccagtgctt
    32281 tattatagtc ccctgtcaca aaaaaggcag ttgttcggta ggagagataa cctggaacca
    32341 gcggtgccaa aatggccaag ataaagacca cagcaggtgt cttataaaga atacttaaaa
    32401 tctggctgac acaagaacca ataatggctg caatgaaggt agctacaatg acattggtcg
    32461 gttccttgag caagagatag attagccaga cagtcatgcc caaaatccct ccaggtaaga
    32521 gcatagaccg ttgcacattg agtacgatta aaaaagtgat aatggcaaga aaacttgcta
    32581 ctgcttgtaa taaaaaggtt gttagtgtca tattagttca tcaataccaa ggcgacagaa
    32641 gttcctgccc ctaaagcgag ggtaatgagc agggattcaa acatcttact cataccagag
    32701 tttatgtggt tggtcataat atcacggacc gcattggtca aggcaatacc tggtacaaac
    32761 ggcatgaccg caccagctat aatcaaatct gccgttgaag gaaaacctgt gtagcgagcc
    32821 caaaactggg caattatccc aaagacaaag gctccagcaa aggctgtcac aaagggaatt
    32881 cggataaatt tttccacata gagggaaaag gcaaaaccaa ataaggtcgc cactcctgcc
    32941 ccaagtgcgt cgtagatatt tccgctaaac ataactgaaa agaaaggagc actaaaggtc
    33001 gcagccagag ttacctgcaa cttagtatag ggaaggggtt gagcttgcaa ggccgtcaat
    33061 tgcttaaagg ctgtttctaa gtcaatctgc cccccaacta gctgacgaga aatctggttc
    33121 acatcgcaga ctttttcgat gttataagaa gaggaggtca cgcgcttcat gcgcgaaata
    33181 ttggtatttt caatagagaa aaagatagcg gcaggcatgg caaggacatt gcaatccaca
    33241 atcccctgcg aatgcgcgat tcgaatcatg gtatcttcta cacgatggat ttctgagcca
    33301 cttttaagga gaatagtccc cgctagcata atcacatcaa tgacggcatt taattctttt
    33361 gattcttcca tgctttcctc cttttatcaa ctccctctat tctatcacaa atctggactc
    33421 aaaaaaaatc tttgccatga aatcatgaca aagattgatt actcattttg attatccatc
    33481 tacttttaag gagtagctga agttgtttta ggtttgtaga ttgaaatctt gactctagtc
    33541 ttattgaggt ctaccttttc acctgctcta ggactttgtt caacaaccat gccttctgca
    33601 ctacctgcag gcgctgtcgt cacttctaca acttctatat tagcttcctt aatcccaaca
    33661 atttgaatca aattgttctt agtaaactcc aagctggaac caatgtaact cggcatggca
    33721 acacttgtaa cttttttagc tactgtcaag acaatttgag taggtttact cacatcataa
    33781 gtcgttccgg cacctggact ttgtttcata atcgttcctg gttcgctttc gctggactct
    33841 tcttcctcta tcttaatcaa attctcagga accttcttct gcttgagttc tgagattact
    33901 tctgtagagt tccgtccaat atagttccct aattgaatcg tcgtagcttt tttagctact
    33961 gtcaaaataa tttgagttgc cttgctcaag tcataggtcg taccttctgg tagactttgc
    34021 ttcaggaccg ttccagcctc actctcattc gactcttctt cctcaatttt aatcaaatta
    34081 tctggaactt ttttctcttt taattccgcg ataacatcag aagatttccg gccgacataa
    34141 ttactaattt ggaaggattg tttgcctgat gagacaacca gattgatttt cgttccttct
    34201 tttcgaccag ttccagcgcc aggatctgta cggataatcc gcccttcttc caccttttca
    34261 ctagcctctg tcttctcctc accaatctca aaattggctt ttttgagcgt tgccttggcc
    34321 tctgcaactg tctgacctgc cacatctgga atggcaatgg ttgcaggagt tctggatagt
    34381 atccaaataa gagaagctgc caccaataca aggctggcca acaaaatcag gtaacgcatc
    34441 ttaaatctat gttttttcgg tgcttgtggt tggtaagttt cctctgtcac agcctggctt
    34501 gggtttttga ttgatttgtg ttctgtctgc gcttgaacct taggaataga tgtcaaggta
    34561 ctctgagaaa ccttcggcaa ggtcttggta tctgccttgc tcgtttcatc aaagattaac
    34621 ttactttcat ttctacgatt gtaggacaag ctactagaca agtccacata catctctgaa
    34681 accgagcggt agcgattggt caacttttta gcagttgcct tgataataac attttctaaa
    34741 gcctgaggta cagatggatt ttctgcaata acggacggca ggggtttctg gaaatgctgg
    34801 agggcaatgg tcaccgcgct atccccgtca taagggatat ggcctgtcaa catctcatag
    34861 aaaataatcc ccatggcata gatatcactc tgcacagtcg ccttagaacc acgcgcctgc
    34921 tctggtgaca agtaatgaac tgagcccaac atcgagttag tctgggtcag acttgtctct
    34981 gcaaaggcta cagcaatccc aaagtctgtg accttggcag tcccatctgg tgtcaaaagg
    35041 atattttgag gtttcaagtc cctgtgaaca attcctcgag tatgggccaa gcgcatagcc
    35101 aagagaattt gtcccatgat acggactgct tcttcattag aaagaggata atgttccttg
    35161 atatagcgtt tgaggtccag tccagccaca tactccatag ctaggtactg ttgaccgtct
    35221 tcctcgccaa tatctgttat ccgaacgata tgaggatggt ctagatctgc catagctctc
    35281 gcttcacgct gaaaacgagc tacagctatc gggtccgtct ggtagttggt cctcagaacc
    35341 ttcactgcca cttcttcccc atctaagatt aagtctttgg ctaggtagac atccgccatg
    35401 cctcctcgac caatctgttt gacaatccga tagcgtccgg caaaaatctt gccgatttgg
    35461 atcattctgc atcctcctcg ttcatagaaa caagggcaac cgtaatgttg tctaaacctc
    35521 ctgcattgtt agcaaaacga acaagtgtct ccgttttatc tgctaaagga atatcactgg
    35581 ttacaatatc acgaatctca ctgcctgaaa tcatgttggt caagccgtca ctattgagca
    35641 agagatagtc acctgactca aggataactg tcccaaaatc aggctgaatt tcatcttttt
    35701 gcccaataga ctgggtgata atattttttt gcggatgagc ttctgcctct tctggtgtca
    35761 attgaccagc cttgagcaat tcattaacca aggaatgatc gctcgtcaac tgatggtatt
    35821 cttctccacg aatcaagccg atacgcgaat caccaatatg agcatagata gcctgattat
    35881 caataatagc aaggacttcc aaagtagttc ccatgcctct gtaagcttca tcctgaccaa
    35941 gctggtgaat cttttgattt tcaatttcta ggtaatgggc gaaccattca cgcacttcat
    36001 tgactgtatc gatctgagta tcaacccaag ctacacccag gtctgtgact gccatttcac
    36061 tagcgatatt ccctgcgcga tgacctccca tcccatcagc taaaataatc atggtacgtc
    36121 cagctctatt gacatagtgg ttgacatagt cttggttatt tgttcgtttc tgaccaacat
    36181 ctgttaataa tgaaatttcc atgtgtcagt tccttcctaa tccgatatct tgcgaaattg
    36241 actgatgaag aatccatcac ttccatacaa ttcaggtgta atgaggatac agccgtcttt
    36301 catgatatcc ttacattcat gttctagttt tacctgctcg aactcgggat gactctctaa
    36361 aaaggcctta acgacttgaa aattctcctc tgagacgata gtgcaggtgc tataagttat
    36421 tataccacct ttgcctagta tttgacaaac actacctaat atttctaact gaatttcctg
    36481 caaggacgcg aaatctgccg tttctttatt gtatttgata tctggttttc ggcgcaaaag
    36541 accgattcct gaacaaggag catccaccaa aatcttatca aaggaatcct ggtcaaaaaa
    36601 ctcatgcacc tttctggcat ccaatttttg agtttgaacc cgatcttcaa ctcccagacg
    36661 ttgggcattt tcttgaatta aatccaactt atggtcgtac aagtccagag cagtaacctg
    36721 acctgtcgta agataagagg ctatatgggc tgttttccca cctggagccg cacaggcatc
    36781 aagcactcgc tcatcacctt gtaaatcaag cgtcggagca accagctgac tggactcatc
    36841 ttggatggta atggctccat tcgcaaacaa attatgccct gcaaaatgcc cctgctcctt
    36901 aaccagacca gtggttgcta aaagggaatt attcgcctcc aacaaggctt ggatttcctc
    36961 ttttcgactt aggtctgtta cacgaatact ggctttgttt cgcactaaca ggctttcaaa
    37021 gatggctttt gctctctcct ctccgtattc ttccttgagt ttggcaacta gccaaactgg
    37081 gagagaatag gcaatggagt cacgcttgtt ttttcgcttg atgctagcaa tatctggcca
    37141 gccttcacgc aagatacggc gaaggacagc gttgaccaat ttttcactgc cttttttacg
    37201 gagtttggcc aattccactg cttcattgac cacagcatga tctggaatct tgtccaaata
    37261 gcggagttgg taggcactca tgagaagaag gacatagagc cagctgtcta actggtctct
    37321 gtcttcgata aagtgggata ggtaccattc cagagtcagt ttacgggcta ccgttccata
    37381 gactagctcg gtcactaagc ccttgtctgc tgcagagagc tgactcccct tgaggtgttt
    37441 atttaaggca atatttgaat atgcttgatt cacaaaaaca tcctctagca ctgctagagc
    37501 taaacttcta gccgtttcta ctttagtcac caaatcgttc tcctacagtc aatgtacgtc
    37561 caactccgtt gaggaaggaa gcaatgtcca tcttaggctt accagctggc tgcacttgtt
    37621 tgagggatag agccccttca gccgttgcga caatcaattc tttcttgcag atagagagaa
    37681 tctcacctgg atttccctga ccttctactg gtagggcttc ataaatctta aagcggtcgc
    37741 ccttaaggaa agtatgggca acaggccagg ggttcattcc acgaatttgg ttaaagagtt
    37801 gacgattggt tttgttccag tccagttttt cttcctctgg ctttatattt ggagagaagg
    37861 taacctgact cgtatcctgc ggttcaggtt tgatatcacc agcaatatag gcaggcagag
    37921 tgtccaaaag caaatcacga ccaactagcg ccaatttttc aaacaaggtg ccaacattgt
    37981 cctcatctgt gatcggaatg ctgcgacgag aaatcatatc tcctgcatcc atttccttaa
    38041 ccatttccat gatggtcaca ccagcttcct catccccttg aatcaaggca taatggatag
    38101 gcgcaccacc acggtgtcta ggaaggaggg aggcatgaac gttgacagca aagtccatgc
    38161 tatcaaggag tttgcttggg agaaactgcc caaaagcagc agtcacaatt ccatctgctc
    38221 ctagcttcat aagttcttcc atctctggac ttccagataa tttttcaggt tggtagatag
    38281 atagtcctgc ttccttggca gcctgcttga ctggggtttc ttggataact tttttacgac
    38341 caacagcacg gtctggctgg gtcacaacgg ctagaatttc gtaacggtca tctgtcaaaa
    38401 gtccttttaa gactgttgct gaaaagtcgg gggtccccat aaagattagt tttgtcatat
    38461 cttctccttc ttataaaaat tgctgcggct catggtcaat gctgagacgg agctcactat
    38521 tttcccgttc ttgagtcaag gctaaaacct ggttgagggt cggccccagc tcatcttcta
    38581 aacggtattt aattaaaatc tggtaatgat agaggttgtg ggtacgggca atcggttttg
    38641 gcgttggccc cagaatggga ctggtctctg acaagcctga ccgcaaaatg ttcatgactt
    38701 cataggcacg tttgaaaacc tcttcttctt tcttgtgaga aagggtaata ccaatcgtga
    38761 aatagtaagg tggatagccg agttgtcgtc tgattcccat ttcataggca taaaagcctt
    38821 cgtaatcttg atccttggca aatcgaatag catagtgctg cggattgtag gactgtatca
    38881 agacttgacc tgccttttca gcccgacctg ctcgacctgc cacctgagtc aagagctgga
    38941 aggttctctc agaagaacgg aaatcaggca gattcaaggc cgtatccgca tttagaactc
    39001 cgactagggt aacattggga aaatccaaac cctttgcaat catctgagta ccaagtaaaa
    39061 tatccgcttc ccctcgccca aactggtcaa gcaaggcttg gtgactgcct ttctttcgag
    39121 tcgtatccac atccatcctc agaatgcgag cttggggaaa gagttctgct agctcatcat
    39181 aaaccttctg agttcccgtc ccatagtaac gaatactgtg gctcttacag ttaggacaga
    39241 cctgaggaat atccttcgag aaaccacaat aatggcagtt catagtcttg gtatccatat
    39301 gcaaggtcag agaaatatcg cagttgggac aagtatccac cgtcccacac tcccgacaca
    39361 tgacaaagct agaataacca cggcgattga gcatgagaac cacctgctct tttttaacca
    39421 gacggtcttg gatagcctct agcaaaggag gcgtaaagtt tgacgtctca ttttgtccga
    39481 tatagtctcg aaagtcaatc acttgaacct cagggattgt agccaaagga ttggcacgtt
    39541 gggttagacg taagtgttga tagacgcctt tgccagcacg cgcccggctc tctaagctcg
    39601 gcgttgcaga tccaagtacc agagttgctt gattatactg agcccgtaaa atagctacct
    39661 ctctggcatg gtaacgggga ttgctttcct gcttataagc cgcttcatgc tcttcatcaa
    39721 taatcatgac acccagattt ttcagaggag caaagatagc agatctggca ccaacaacaa
    39781 cttgggcatc gccacgctcc accttgcgcc attcatcata cttttcacca ttggataatc
    39841 ctgagtgaag aatggctacc ttgtccccaa agcgtgctat aaaacgctcg gtcatttgag
    39901 gagtcaagga aatctcaggt accagcaaaa tagctgtctt gcccttatcc agggcacctt
    39961 ggataatctg caagtaaacc tcggtcttcc cacttcctgt aatcccttga agtagaaagg
    40021 gaggttgaga actgccaata gaactcacaa ccgcatcacg cgcctgtctt tgttctggat
    40081 ttaactccaa aggtctactt gcttcaattc cttcaaaata agcagccgag cgttgaactt
    40141 ccttttggac tatggtaaca gcaccttgat ccacaaagaa gttgacttgc tctcgcgagt
    40201 aggactccaa caagctagcc aaggaagcgc tctctggatg agacagcaga taatctctca
    40261 gttccaactt tttcttggca cgtgtagaaa tctcaacacc ttctaattga gcatggtcaa
    40321 cctcatacca agactgggtc ttgaccttct tttgatcgac tgcctgatat tccagaccaa
    40381 gcaggccttt tctagtcaaa cgcatcattt cagcttgctt acctaaatcc agagaagaaa
    40441 aggctagcga atcttctgaa ccaaacaggc gtactcgttc ttcctgactc aagccttcca
    40501 gaggatagag aatcttgtca tagctagaat tcagaaatcc cggaagcatg gccttgagga
    40561 tagagatttt gtaggagaag acagatttgc gtaactcctc agccagccag agttgttctg
    40621 gcgtgagaac aggagaaaaa tccagcacct ctgcaatatc ttttaaatct tgctccatct
    40681 cttctccatc tgattgggac tccaaaccaa gaacaatccc ttgaatcagg cgattaccct
    40741 taccaaaagg cacatgaacc cgcatcccaa cttccagcat tccctcaaat tcctccggaa
    40801 tcctgtaact atagggctgg tccgtctgca tcaagggcac atctacgata atcttagcta
    40861 gggccatctt ctcacctcct ccttgtcagt acattcttgc aatagaaaaa ataagattga
    40921 gtccccccaa ccttaaattt tttcaccatc ttctttttct ttagcaattt gctctttgat
    40981 tttcttttct tcttcttctt tgcggcgttt ttcttcttcg atacggcgac gcactgcttc
    41041 acgttttcct tctggatctg ggtgaattgt aacgtttcct gattcgattt cttctaaagc
    41101 gcgaagagtt gatttttcag acttgaaacc ttgagttgct ggggcacctg cttccaattc
    41161 gtgggcacgt tttgcttcca agattacgag tgaatatttt gaaggaacct tgtcgagcaa
    41221 ggtatcaata gagggtttta acatcatttg cttgtaccta ttttctaaat tttatcgggt
    41281 agttggagat tttggtaaca tctcctgata gtgaccaatg acacgatcca cacagaagtg
    41341 ttctgcttca atcacacatt tgacacgttc agcagctagg ggcacctgat cgttgacaat
    41401 cgcataatca tactcacgca tgagggcaat ttcttccttg gccttttcga ttcgttgggc
    41461 aatcacttct gcactatctg ttccacgacc taccaagcga tcttgcaatt catccaaatc
    41521 tggtggtgtc aggaagataa agacagcatc tggaaccttt ttcttgacct gaagagcacc
    41581 ctgaacttca atttcaagga aaacatcgat tcccttgtcc aaggtttcat tgacataggt
    41641 cagaggagtt ccatagtagt tgccgacata ttctgcgtat tccaacatct gtccttgacg
    41701 aatcagctct tcaaattctt cacgagtacg gaagaaatag tcaacaccgt ccacttctcc
    41761 aggacgttgt gcgcgtgtcg tcatcgatac agagtattga aattggtttt cagaactctc
    41821 aaaaatctct cttctaaccg ttccttttcc aacccctgaa ggaccagaaa aaacgattag
    41881 taagcctcgg tctgccattg tgtctccttt tagtcaatct gtgaaataac atttctctag
    41941 aataatggca aaaagccaga ttatccttta cagtctttct atctagtgta acaaaaaagc
    42001 agtaattttt caactgctct ttcttattta tttagcataa tctactgcac gaagctcgcg
    42061 aatcacggtt accttgatat ttcctggata atcgagattg ttttcaattt tcttacgaac
    42121 tttgtgagcc aagattgtga ctttgtcgtc cttgattttt cctggattga ccatgatacg
    42181 aatttcacgt cctgcttgaa gggcaaagct agtttgcact ccttcaaagc cgttagcaat
    42241 ttcttccaaa tcatggagac gcttgatgta gctttcaaga gactcactac gagcacctgg
    42301 acgggctgcg ctcaaggcat ctgctgcagc gacgataact gctatcacgc tctcagcttc
    42361 aacatctccg tggtgactag caatcgtatt caccacaact gggggttcct tgtacttacg
    42421 ggccaattcc ataccgattt caacgtggct accttcaacc tcatggtcaa tggctttccc
    42481 gatatcgtga aggaatccag cacgacgggc aagagccgca ttttcaccaa gttcgctcgc
    42541 catgatacca gccaacttag caacctcaat cgaatggcgc aaaacatttt gtccatatga
    42601 agtacggaac tgcaaacgtc ccataatctt catcaagtct ggatgaaggt ttggcgcacc
    42661 aatttcatag gcagcagcct caccgtattc acgaatctta ttgtcaatct cttgacggtt
    42721 tttctcaacc aactcttcga tacgagctgg atgtatacga ccatctttga gcaacatttc
    42781 catagtcata cgggcaatct cacgacgaat cggatcaaat cctgacaagg tcaccacttc
    42841 tggtgtatcg tcgataatca catcgacccc tgtcaaactt tcaaaggtac gaatgttacg
    42901 accttcacga ccaataatgc gtcccttcat agtatcgtct ggcagatgaa ctgttgagtt
    42961 tgttgactcc gctacatatt caccagcgat acgttgcata gcttgaacca agatgtcctt
    43021 ggccattttg tcagaacgtt ccttgacctc ttgctcagct tcgcgaatgc gactggcaat
    43081 ctccctggtc aagttttcct ctgtctgagc caagataata tctcgtgctt ctgcctgaga
    43141 cagagcacca atacgctcta gttctgcttc tttttgtctt tcgacttcct ctaattgctc
    43201 ttcacgcgca tcaaggtttt tcgctctatc agaaatactt tgttcttttt gttcaagtgt
    43261 ttgttcttta ctcgtcaaat tgtcgtcctt acggtcaagg ctagtagctc tctctgtcaa
    43321 acgactttcg atttgtttga gttcttgacg ttctgattta aattcagcgt ccacttcttc
    43381 acggtatttt ctggcttctt ctttggcctc caatagtgct tcttttttaa gagacttgct
    43441 ttcacgtttg gcttcattaa caagtaaatc cgcttcacgc tcagcttgtc cacgtaaatt
    43501 agttgcttct tgttcagcat ttaaaagcat caactctgca gcttcatgag atgatttcat
    43561 cttagctgag atgctgacat atccaatgac taaaccaatg atgacggcaa aaacagcaat
    43621 cgcaagcgac atgatctcca tgtttttacc tcattttatt gttattccga atgacataca
    43681 ttcttttaca ttctaccata aaaaagtgat tttcacaaac ctaaaataga atatgttttg
    43741 gggaatttgg aacacatttg ccaaaataaa cttgtttttt agaaatagta gtttagtaga
    43801 gacttgagaa aaagcctacc tttcaataga cttagtaatg atctttaaag gacaagaaag
    43861 ccacgctatc tccatccatc atataaatca agcgattttc tgcatcaata cgccgtgacc
    43921 aggctccttg gtaatcatat ttgagtggct ctggttttcc tattcctgta aatggatcgc
    43981 gttgaatatc cttgattaat ttattgattc tttttagtgt ttttttatct tggttttgcc
    44041 agtagcaata atctgcccac gcatcttctg taaacttgag tagcattcat cactcctcaa
    44101 tcacatggac ctgagtgctt ccagcacgga cttgagccat tcctcgcaaa accttgtctg
    44161 ataactcctt attttgggcg attctcaggg tttcctgaat actgtcccat tcgctctttg
    44221 aaagaactac aatgtcctca tctggatttt tattgaccac cgtcaaaggc tcaaattcat
    44281 catttacctt cttcatgtag tcctttaaat gatttcggaa tgttgagtaa aggactgctt
    44341 ccataaccat acctcgtttt agctcttttc cactattata cacgaaaaga aagaaattgt
    44401 caggaacttg tacaagattt tcttttctat ctatttatac tcaatgaaaa tcaaagagca
    44461 aactaggaaa ctagccgcag gctgtacttg agtacggcaa ggcgacgttg acgcgatttg
    44521 aatttgattt tcgaagagta ttattcgtaa aaaatctcaa aaagcctacc tttcggtaga
    44581 cttagcttgt ttctattcta atcggcactc ttccaaaatt ttgctctgct atacttggct
    44641 ttcctagttg gtaaatctgg tctgcttttt gggtcatagc atcccaaggt tctttgccaa
    44701 ttcggctgac tagattgacc tgtcctttca gagacttgag atgttgcctg cctttttcag
    44761 taaatccaag gacatgaatg gcttctggca agacattttc tctagcctgc accaaaatat
    44821 aggtcaatag gcgtctgaca cgcgccttgg tgtaacgttt ggtcgcaacc gcctcgacca
    44881 actcttctac agactgggct gttttaatag cttctttaat gcgcacagcc atttcttgat
    44941 tgacctgata gatggtggtt aggtcgggat ttgacaagat ttgatagcgg agcaagggaa
    45001 aatagtcttc ccagatcacc ttactggcct gctcaaatag ggcaacagaa ggcataaagc
    45061 gttctaagaa atcttggtcc ttctgatgtt gacggagggc tgtcgccgag gcaaagtcca
    45121 catctttatt cacagaatgg taacctgccc cctgacgctg aatcggatgc agcttgatat
    45181 ttcgtcctgc aactgccttg gcataggcca gagcaagaac atgattgggg gtattacctg
    45241 aaaaatcaag accagcaaat tccttccaca tagcttgggt tttctgggga taggaaaggg
    45301 aatcaggcag attttccaca aatttctcca tctcagcacc tttctctgtg tataagtcag
    45361 cgattttttg ataatctaga acttcttctg tcccaaaagc gaggctatct atgcccaacc
    45421 gagctaagat atccacggcc ccttgaccga aaaaatctgc cgcctgaacg ctgactaaaa
    45481 agggcaattc cactaccaga tccgctccat tttccagcgc catctgggct cgtgtccact
    45541 tatccacgat agcaggttct ccacgctgca tgaaattccc tgacatagcc acgattttca
    45601 gcccctccgc ttgatccagc aggtatttgt gtccattatg aaaaggattg aactccgcga
    45661 taatacccgt gatggtcata gtaatctcct acttctttag tatcaaattg agtcaattat
    45721 attctgtagg ctccgttttt gagtagttgt ttcctcaatg aatttaattg accattcttt
    45781 taagtaattg tgtctactaa aataccagcc atttatccac ttatttatta aagttttttc
    45841 atcatcgttc aacatgttat aatattttct ttcatttatc
//
LOCUS       DAVLYV010000008        45537 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLYV010000008 DAVLYV010000000
VERSION     DAVLYV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45537
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.008"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(89..2086)
                     /gene="metG"
                     /locus_tag="VYO70_000441"
     CDS             complement(89..2086)
                     /gene="metG"
                     /locus_tag="VYO70_000441"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1712727.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQVYVDRMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEVSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(2210..3469)
                     /locus_tag="VYO70_000442"
     CDS             complement(2210..3469)
                     /locus_tag="VYO70_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712728.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(3471..4172)
                     /locus_tag="VYO70_000443"
     CDS             complement(3471..4172)
                     /locus_tag="VYO70_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712729.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(4156..5355)
                     /locus_tag="VYO70_000444"
     CDS             complement(4156..5355)
                     /locus_tag="VYO70_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1712730.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            5641..6987
                     /gene="gor"
                     /locus_tag="VYO70_000445"
     CDS             5641..6987
                     /gene="gor"
                     /locus_tag="VYO70_000445"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1712731.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGVKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQVAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFTSMYSACTCNRQETRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            7184..7720
                     /locus_tag="VYO70_000446"
     CDS             7184..7720
                     /locus_tag="VYO70_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1712732.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            7775..7855
                     /locus_tag="VYO70_000447"
                     /pseudo
     CDS             7775..7855
                     /locus_tag="VYO70_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(7924..8139)
                     /locus_tag="VYO70_000448"
     CDS             complement(7924..8139)
                     /locus_tag="VYO70_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1712733.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            8237..8557
                     /locus_tag="VYO70_000449"
     CDS             8237..8557
                     /locus_tag="VYO70_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712734.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(8587..8925)
                     /locus_tag="VYO70_000450"
     CDS             complement(8587..8925)
                     /locus_tag="VYO70_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712735.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(8937..9656)
                     /gene="trmD"
                     /locus_tag="VYO70_000451"
     CDS             complement(8937..9656)
                     /gene="trmD"
                     /locus_tag="VYO70_000451"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1712736.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRVQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(9646..10164)
                     /gene="rimM"
                     /locus_tag="VYO70_000452"
     CDS             complement(9646..10164)
                     /gene="rimM"
                     /locus_tag="VYO70_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1712737.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(10269..10554)
                     /locus_tag="VYO70_000453"
                     /pseudo
     CDS             complement(10269..10554)
                     /locus_tag="VYO70_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(10555..10983)
                     /locus_tag="VYO70_000454"
     CDS             complement(10555..10983)
                     /locus_tag="VYO70_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1712738.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKKLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEE"
     gene            complement(11560..11799)
                     /locus_tag="VYO70_000455"
     CDS             complement(11560..11799)
                     /locus_tag="VYO70_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1712739.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(11819..12091)
                     /gene="rpsP"
                     /locus_tag="VYO70_000456"
     CDS             complement(11819..12091)
                     /gene="rpsP"
                     /locus_tag="VYO70_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1712740.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            12270..12470
                     /locus_tag="VYO70_000457"
     CDS             12270..12470
                     /locus_tag="VYO70_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712741.1"
                     /translation="MNTILEKFYKEHQVKPFISPERDLDTWLLSPKPVPKRNMDLLAD
                     DSLAGDIILLWRIQFGTFTTET"
     gene            12949..13161
                     /locus_tag="VYO70_000458"
     CDS             12949..13161
                     /locus_tag="VYO70_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712742.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            13244..14047
                     /locus_tag="VYO70_000459"
     CDS             13244..14047
                     /locus_tag="VYO70_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1712743.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITINSIEVVKDYDFKS"
     gene            complement(14104..15645)
                     /locus_tag="VYO70_000460"
     CDS             complement(14104..15645)
                     /locus_tag="VYO70_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1712744.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(15647..16129)
                     /locus_tag="VYO70_000461"
     CDS             complement(15647..16129)
                     /locus_tag="VYO70_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1712745.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(16129..17214)
                     /gene="rlmN"
                     /locus_tag="VYO70_000462"
     CDS             complement(16129..17214)
                     /gene="rlmN"
                     /locus_tag="VYO70_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1712746.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDSVEQALE
                     LTELLKNIKKLSYANLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(17235..17765)
                     /locus_tag="VYO70_000463"
     CDS             complement(17235..17765)
                     /locus_tag="VYO70_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1712747.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(17922..18527)
                     /locus_tag="VYO70_000464"
     CDS             complement(17922..18527)
                     /locus_tag="VYO70_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1712748.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(18700..19737)
                     /locus_tag="VYO70_000465"
     CDS             complement(18700..19737)
                     /locus_tag="VYO70_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1712749.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(19771..20706)
                     /locus_tag="VYO70_000466"
     CDS             complement(19771..20706)
                     /locus_tag="VYO70_000466"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1712750.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            complement(21259..22449)
                     /locus_tag="VYO70_000467"
     CDS             complement(21259..22449)
                     /locus_tag="VYO70_000467"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1712751.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            complement(22664..24007)
                     /locus_tag="VYO70_000468"
     CDS             complement(22664..24007)
                     /locus_tag="VYO70_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1712752.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYWLTQLMQPYLAMIFVNTKTRADELHSYLTSQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            complement(24147..24962)
                     /locus_tag="VYO70_000469"
     CDS             complement(24147..24962)
                     /locus_tag="VYO70_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1712753.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            complement(25014..27194)
                     /locus_tag="VYO70_000470"
     CDS             complement(25014..27194)
                     /locus_tag="VYO70_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1712754.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            complement(27480..28406)
                     /locus_tag="VYO70_000471"
     CDS             complement(27480..28406)
                     /locus_tag="VYO70_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712755.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            complement(28399..29091)
                     /gene="ftsE"
                     /locus_tag="VYO70_000472"
     CDS             complement(28399..29091)
                     /gene="ftsE"
                     /locus_tag="VYO70_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1712756.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            complement(29109..30204)
                     /gene="prfB"
                     /locus_tag="VYO70_000473"
     CDS             complement(join(29109..30131,30133..30204))
                     /gene="prfB"
                     /locus_tag="VYO70_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1712757.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKMSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSYEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            complement(30312..30968)
                     /locus_tag="VYO70_000474"
     CDS             complement(30312..30968)
                     /locus_tag="VYO70_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1712758.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIVRTSAKVL
                     "
     gene            complement(31382..32092)
                     /locus_tag="VYO70_000475"
     CDS             complement(31382..32092)
                     /locus_tag="VYO70_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712759.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGS"
     gene            complement(32092..32856)
                     /locus_tag="VYO70_000476"
     CDS             complement(32092..32856)
                     /locus_tag="VYO70_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712760.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            complement(32856..33812)
                     /locus_tag="VYO70_000477"
     CDS             complement(32856..33812)
                     /locus_tag="VYO70_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1712761.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            complement(33816..34685)
                     /locus_tag="VYO70_000478"
     CDS             complement(33816..34685)
                     /locus_tag="VYO70_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1712762.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            complement(34953..36113)
                     /locus_tag="VYO70_000479"
     CDS             complement(34953..36113)
                     /locus_tag="VYO70_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1712763.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(36214..36462)
                     /locus_tag="VYO70_000480"
     CDS             complement(36214..36462)
                     /locus_tag="VYO70_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1712764.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(36541..37131)
                     /gene="clpP"
                     /locus_tag="VYO70_000481"
     CDS             complement(36541..37131)
                     /gene="clpP"
                     /locus_tag="VYO70_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1712765.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(37305..37934)
                     /gene="upp"
                     /locus_tag="VYO70_000482"
     CDS             complement(37305..37934)
                     /gene="upp"
                     /locus_tag="VYO70_000482"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1712766.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(38023..38490)
                     /locus_tag="VYO70_000483"
     CDS             complement(38023..38490)
                     /locus_tag="VYO70_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1712767.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(38509..39066)
                     /locus_tag="VYO70_000484"
     CDS             complement(38509..39066)
                     /locus_tag="VYO70_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1712768.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            39191..40036
                     /locus_tag="VYO70_000485"
     CDS             39191..40036
                     /locus_tag="VYO70_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1712769.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            40095..41270
                     /locus_tag="VYO70_000486"
     CDS             40095..41270
                     /locus_tag="VYO70_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1712770.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            41290..41745
                     /locus_tag="VYO70_000487"
     CDS             41290..41745
                     /locus_tag="VYO70_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1712771.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWPGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(41765..42514)
                     /locus_tag="VYO70_000488"
     CDS             complement(41765..42514)
                     /locus_tag="VYO70_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1712772.1"
                     /translation="MYHIKEAAQLSGISVKTLHHYDKIGLLVPLKSENGYRTYSQEDL
                     ERLQVILYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLDTLISTLQKT
                     IQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKGHEDEATAAF
                     NQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIGKGYVYNPEF
                     KENIDKFGSGTAQYTSDAIAAYVQTNAEKIG"
     gene            42742..44077
                     /locus_tag="VYO70_000489"
                     /pseudo
     CDS             42742..44077
                     /locus_tag="VYO70_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(44117..45061)
                     /gene="manA"
                     /locus_tag="VYO70_000490"
     CDS             complement(44117..45061)
                     /gene="manA"
                     /locus_tag="VYO70_000490"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1712773.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(45182..45478)
                     /locus_tag="VYO70_000491"
     CDS             complement(45182..45478)
                     /locus_tag="VYO70_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1712774.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYKDVIDSNGVSIE"
BASE COUNT        13250 a         9925 c         8419 g        13943 t
ORIGIN      
        1 acctgcggct agcttcctag tttgctcttt gattttcatt gagtataaat aaaaaaacag
       61 aactagcctg aactagtcct gtctactttt acccaatcac acttccattt ggtacagctg
      121 gatcaactgt gagaagggtt aatttgccat catgttcagc tgagagaatc ataccctggc
      181 tgacatattt tttcatcatt ttacgtggtt tgaggttagc aacgatttga actttcttgc
      241 cgaccaattc ttgttcattt ggatagtatt ttgcaattcc tgaaagaatc tgacgatctt
      301 ctccatcacc agcatccaag cggaattgaa gcaacttatc tgaaacttct actttagaca
      361 cttctttgac ttctgcgaca cggatttcaa ccttgtcaaa gtcttcaaac ttgatttcat
      421 ccttgtttag tttgagctca acttcgtccg gattccattc tttttcgact gctggtttat
      481 tgccttccat ttgttccttg atataggcga tttcttcttc catatttaga cgtggaaaga
      541 taggtgttcc tttggcaact acagtcacat ctgctgggaa gtcagccaaa ctcaagtttt
      601 caagactaga aacttcttcc aaaccaagtt gagtcaaaac tgcacgacta gtttccatca
      661 taaatggttc aatcaagtga gcaactacac gaatgctggc tgccaagtgg ctcatgacac
      721 ttgccaattg gtcacgaaga gcttcatcct tggccaagac ccatggtgcg gtctcatcga
      781 tgtatttatt ggtacgagag atcagagtcc agactgcttc aagcgcacgt ggatagtcaa
      841 ctgcttccat gtgtgtatgg aagtctgcga ttgattgttc tgcaacctca gcaagaacat
      901 gatcaaattc agtcacacct tctacatagg cagggatttg tccatcaaag tacttattaa
      961 tcatggaaac cgtacggtta aggaggttcc caaggtcatt agccaattca tagttgatac
     1021 ggccgacata gtcttcagga gtaaaggttc cgtctgaacc aactggaagg ttacgcatga
     1081 ggtagtaacg aagtggatct agtccataac gctctaccaa catttcaggg taaacgacat
     1141 tcccttttga cttagacatt tttccgtctt tcatgacaaa ccaaccatgg gcaatcaaac
     1201 gatcaggtaa tttaacatcc aacatcataa gaaggattgg ccagtagata gagtggaagc
     1261 gaaggatatc ttttcctacc atatggaaga ctgttccatt ccagaacttg tcaaagttac
     1321 catgttcgtc ttgagcgtag ccaagagctg tcgcatagtt aagaagggca tcaatccaaa
     1381 cgtagacaac gtgttttgga tttgatggga caggcactcc ccatgtaaag gttgtacgag
     1441 ataccgccaa atcttccaag cctggctcga tgaagttgcg tagcatttca ttaaggcgac
     1501 catctggcgt gataaattca ggatgagctt tgaaaaattc gaccaaacgg tcttggtatt
     1561 tgctaaggcg aaggaagtat gattcttcag aaacccattc aacctcatga cctgatggag
     1621 caataccacc agtcacattt ccagcttcat cacggaaaac ttctgccagc tggctttctg
     1681 taaagaattc ttcgtctgat actgaatacc aaccagagta ttcacccaag tagatatcat
     1741 cttgagcaag taagcgttca aagacctgtg cgacaacttt ttcatggtag tcatcagttg
     1801 tacggataaa tttatcgtat gagatatcta gtaattgcca gagttcttta actccaaccg
     1861 ccattctatc aacatagact tgaggtgtaa taccagcttc ttccgctttc tgctggattt
     1921 tctgaccatg ttcatcaaga cctgtcagat aaaatacatc gtagcccatc aggcgtttgt
     1981 aacgtgctag gacatcacat gcgatagttg tgtaggcaga accgatatga agtttcccag
     2041 atggatagta aatcggcgtt gtaatataaa aattcttttc agacataatt tttcctttcc
     2101 aggcaaatga aacctgtttt tctaacactt cattatatca catttttaat gaatttcgat
     2161 agggaaatcc atacaaaaac aagatagacg agtgtccatc ttgttgatct cattcataac
     2221 gaagggcttc aattggatca agtttcgatg ccttgttggc tggcaagact ccaaaaatca
     2281 taccaacact agccgaaact gcaagactaa atagggcaac tgggattgat actccaactt
     2341 ctataccttc tattaaacct tgcagtaaca aacctgctaa ggcagttaaa ccacttgcaa
     2401 ttgtcaagcc aattaagcca cctaacaagg tcaaaatcat ggattcaatc aaaaactgaa
     2461 ttaaaatatt ggcacgtgtt gcacccaaag ccttacgaag accaatctca cgagtgcgct
     2521 ctgtcaccga aaccagcatg atgttcatga caccagttcc tccaacaaag agagaaatcc
     2581 ctgcgatgga actaataatc gtcgtcataa aactaaacga ttgttgaatt tctgcaaata
     2641 caacggactc atctgccacc tggtattctc cctgttgtaa gcctgcaagc tctgtcattt
     2701 ttcgtgccag ttctggaccc agagttgggg ttaaactggt atcattcact cgaaagacaa
     2761 tactagctat ttcatctata ttaaaattcg cagcaaggga gatattggta gtaataggca
     2821 agccaccaaa cccatatatt tttgaccttt tagcctccgg actagtataa accccaatga
     2881 cccggtaact aaatccattg acttctacaa ccttgttaat agcctcttga ggagattcaa
     2941 ataaactaat ggacaattcc tcatctagca aaataacact tgcaaactct ttgaaatctt
     3001 gctctctcag actacgacct gcaataattt cattcttaac agcatccatg taagttctgt
     3061 ttccacctgt caaattagca ttctcaacct ttttatcttg ataggtcaag atggcattcg
     3121 ttgaattggt tacatagtaa ctatccactc ccttcagttt agctgcctct tgaacccagg
     3181 attcttgcgg ttttggcggt tcaacaggaa cttcctcttc ctttccagaa accgtaaaag
     3241 ctgattgttt ctgagtaaaa gacccatctt tacttttttt aggagagaaa aagacgctaa
     3301 tatttttctg agatttagtc atatctttat tgacttgacg agatagggaa tcacccaaag
     3361 ccataatcac aacaactgat gaaacaccga taataatccc aatcatagta agcaaagaac
     3421 gcatcttgtg agccatgata gatgaaaagg caaatttcag attctgcatc ttagttttcc
     3481 tcctttccta actgagcact gtcagacgaa atgaccccat cccgaatgac aatctgacgt
     3541 ttggcatagg cagcaatctc aggctcatgc gttaccatga taatggtttt tccttcttta
     3601 ttcaaatcaa ccaataattg cataatttgg ttacctgttt tggtatccaa ggctcctgtc
     3661 ggttcatccg ctaggataat agaaggattg tttaccaagg cacgcgcaat ggctacacgt
     3721 tgcttttgac caccagataa ttctgaaggt aaatggtgac tacgttctgt caattcaacc
     3781 ttgtctaaat attcctcagc caacttgcga cgttttgaag acgaaactcc tgcgtaaatc
     3841 aagggcaatt ctacattttg cagagcattg agcttcgata gaagaaagaa ctgctgaaag
     3901 acaaaaccga tttgttggtt acggacctta gccagttgtt tttcaccaag cccagccact
     3961 tcttgacctt caagataata ttctccactg gttggtgtat ccaacatgcc aatcgtattc
     4021 atcagagtgg acttaccaga cccagatggt cccatgatgg ctacaaattc accctcattc
     4081 acttctagat tgatattttt gagaacctgc agttcttggt caccattacg gtagcttctg
     4141 aagatatttt ttagactaat tagttgcttc atcagccttc acctcttttc cttcttccaa
     4201 ggaagatgtt ggattactga tgaccttagc accatttgtc aaaccagaag tgatttcttg
     4261 attttctgcg tcagcatttc ccaatgaaac ctcaactttt ttagcctttt gttgttcatc
     4321 cacaatccag acataatttt tactatcatc cattactaga ctgctaacag gaacaagaat
     4381 agccttagtt ttgcttttaa cctcaatgtt gacagaaaaa ccttgtttca aatcaccaac
     4441 ctcgcctgtc acatcaatag tataagggta tttagaacct gtattattcc cggctgctgg
     4501 actagctgct tcaccattgt ttttaggata gtcagaaata tagcttaatt tcccagtcca
     4561 ttttttatca ggatacactt tagaagtaaa gcttacttct tgacctacag aaaggttggc
     4621 tagattgtac tcagacaatt ctcccttgac ttgtaaattt tcattgctga caatatgaac
     4681 cataacttga ctcgcccctg ttggagattt agaaacattg ctattgactt cgaccacagt
     4741 tccctctagg gtactgagaa cagttgttgc atccaattga ctttgagcct tgcttaattg
     4801 cgctgcagca tctgcacgcg catcacgggc atcacccaat tgagcatcaa tagaagcaac
     4861 agaatttcca gccactggag ttgggctttg caccgttgca tcttctcctc ctactggcgc
     4921 tggtaactgt ggagcctgag ctgaagcggc ttcatttcgt gcttgattga gttcattgat
     4981 atgacgatct gccttagcta ctgctcgact agctgaatca taggccgcct gcgcttctga
     5041 actactgtac ttgactaaag cctgcccttc gctgacctta tcgcccacag aaacaaggat
     5101 ttcatctaaa tcacccttac tagcatcaaa ataaacatat tgttcatttt ttgctgttac
     5161 tgtccctgac aataaaacag aggatgccac gcttccttcc ttggcaacaa caagatgagt
     5221 aggctcatct tttacagcag tctgagaagg ttgtctaaag agtaaaatcc ccccggcacc
     5281 caatacaact acactcgcag caccgattgc tgcatacagt tgccactttt tagctttacc
     5341 attctttttc ttcataatga aactcctttt cttttttaca atactttgct attataccaa
     5401 atttccctcc agcaaacaat gcagttcaga tggaaataat ggcagttcag gattaaacaa
     5461 tcgttcggaa ttttgctttt ctattgtact agatatataa tacactattt tgacttattt
     5521 ggcaagcttt tttgcaattt tttgtgcgta gcagattttt gcaatcaaat caaaaaaaga
     5581 ataaaatata actatcaaaa aagacgctct actatttaaa agattacaaa ggagttttca
     5641 atgagagaat atgatatcat tgctatcggt ggaggtagcg gaggaatcgc taccatgaac
     5701 cgtgctggtg aacatggagt caaagcagcc gttattgagg aaaagaaatt aggtggaacc
     5761 tgtgtcaacg tcggttgtgt tcctaaaaaa atcatgtggt acggggcgca agtcgctgag
     5821 actttccatc aatttggaga agactacggc tttaagacta ctgatcttaa ctttgacttt
     5881 gcaaccctac gtcgcaatcg tgaagcctac attgatcgcg ctcgttcttc ttatgatggt
     5941 agttttaaac gcaacggtgt agacttgatt gaaggtcatg ctgaatttgt agattctcat
     6001 actgtaagcg taaatggtga actgattcgt gctaaacata tcgtgattgc tacaggtgcc
     6061 catccaagta ttcctaatat tcctggtgct gagctaggtg gctcttctga tgatgtattt
     6121 gcctgggaag aacttccaga gtcagttgcc attctaggcg ctggttatat cgccgttgaa
     6181 ttagctggcg tactccacac ttttggtgtc aagacagatc tctttgttcg ccgcgatcgt
     6241 cctttacgtg gttttgattc ctacatcgtt gaaggtttgg tcaaggaaat ggaaagaaca
     6301 aacttaccac ttcacactca caaagtccct gtcaagttag aaaaaactac tgacggcatt
     6361 accattcatt tcgaagatgg tactagtcac acagctagcc aagttatctg ggctacaggt
     6421 cgccgtccaa acgttaaggg cttgcaactt gaaaaagctg gagtgactct gaacgaacgt
     6481 ggctttatac aagtggatga ataccaaaat accgtcgttg agggaatcta tgctctaggt
     6541 gatgtaacag gcgagaaaga actgactcca gttgcaatca aggctgggcg taccctatca
     6601 gaacgtctct ttaacggaaa aacaactgca aaaatggact actcaactat tccaactgtt
     6661 gtcttttcac accctgctat cggaactgtt ggtttgacag aagaacaagc tattaaagaa
     6721 tacggtcaag accaaatcaa ggtttacaaa tcaagcttta cttctatgta ctctgcttgc
     6781 acttgcaacc gtcaagaaac tcgtttcaaa ttgatcacag ctggttcaga agaaaaagtt
     6841 gtcggacttc acggaattgg ctacggcgtt gatgaaatga ttcaaggatt tgctgttgct
     6901 atcaaaatgg gagcaaccaa ggctgacttt gacgcaactg tagcgattca cccaacttca
     6961 tctgaagaat ttgtaaccat gcgttaatcg aaacaaaaga agctgatata aatatgtctg
     7021 cttctttttt aatgattttc gaatcaacaa aaaaattatc caacttgact agtcacgaaa
     7081 atcggctgtt acagtttttg ttaccttgtt tttaaaaaat ggttgacatc gactcctata
     7141 tgagtataat agttaatata cattagaaaa gaggttaact attttgaaaa aagctcacgt
     7201 ttatgctatc cctgctattg gggctgctct cattgctgtt ttggcacaaa tcagtcttcc
     7261 aattggacct gttcccttca ctctgcaaaa ctttgcaatc ggcttgattg ctactgtctt
     7321 tagaccgaga gaggctgtac tttctgctgg actctatctt cttctaggtg ctatcggtct
     7381 tcctgtcttt gcaggaggtg gagctggttt tcaggcttta gttggcccta ctgcaggcta
     7441 tctttggttt tatctcgttt actctggact tacttcctct ctaaccaaca gcaagagtgg
     7501 tgttgttaag atttttcttg caaacctctt gggtgatgcc cttgtctttg tcggcgggat
     7561 tctcagcttg catttcctag ctggaatggc atttgaaaaa gctcttgttg tgggggttct
     7621 tccctttatc attccagacc ttggcaaact tctggctatt agttttatta gccgtcccct
     7681 acttcaacgc cttaaaaatc aggcttactt tactaactaa aaaaggatac cgagttatca
     7741 tgactcaata tccttttttt attttgaaaa cttatactca atgaaaatca aagagcaaac
     7801 taggaagcta gccgcaggct gctcaaaaca ctgttttgag gttgtggatg aaactgacga
     7861 agtcagtaac catacatacg gcaaggcaaa gctgatgcgg tttgaagaga ttttcgaaga
     7921 gtattagttg cctttaaagg catccaccat cgtttgaaat tcttcatttg agagagtaat
     7981 ccctttgccc attttagtat ggtctggact ccaagcacga atatcaaact ttgcaggggc
     8041 accattaaag ctcacacggt taatttcctt ggtccaacct ttttcgtttt cagaaagagt
     8101 caacaagtgc tcttcgattt caaatgtaaa ttctgccatt ttcttctcct tttttagttt
     8161 cattagttta ttcgtaaaat cttgtagatt ttaggaaaat tttatataat attgatataa
     8221 aagaagggag gccaatatga gacataaatt ccagcaagtt ctaaataaaa tacatgattt
     8281 tttaaatgga tatgaccaac ctgaccagac tgaaaccaac tcccttacag ccactattga
     8341 agaggctatc cagaaacaaa ccgctgttca ccttatctta tctgagacaa gctttacagg
     8401 tgacatcatc aaatatgatc agcaaggcca gcaaattatc gtgaaaaatt tttccaaaaa
     8461 tgtgagccgg attatccgta taagcgatat tcaacgcctg cgatttgtcc cctcaactgt
     8521 ccaaacagcc caaaaaaata gatttaagaa agagtgagat gtagttgctt catcccactc
     8581 tttttcttag cgaatttgtt caaaatgtaa atgaactgcg atatgatctc cataaccact
     8641 tctttccaag tcacgttgta aacgatagga aatatagtgt tctgcaatgg taatgtaacc
     8701 tgcgcccaat aaacgatgtt caaccataga ttgaatcata ctgatagtcg cacgttccac
     8761 cttggcttct tgtaaatcca aaactacctt cttagtgact tgagcaagat tttgacgcaa
     8821 atcatctgtc aaaacataaa cagtttgggc tgccttcaag atggcttggt aaatcttatc
     8881 tggattaaat tcagcaattt cgccattacg tttgattact tgcataggtt tctcctttat
     8941 tctttgtttt ctttgatttc tgccagcatt ttttcttctt ctactgtcag ttgataatgt
     9001 tcaagtaaat ctggtctgcg ctcgtaggtt ttctttaaac tctcgtacaa tcgccactga
     9061 cgaatctttt catggtggcc actcatcaat acatctggca cgaccatgcc tcgataatca
     9121 tagggacgtg tgtactgagg atattctaaa agacctgaag aaaaactatc atcttggtgg
     9181 ctagactcct tgccaatcac ttctggaatc aggcgaactg tagcatcaat catggtcata
     9241 gctgccaatt ctccaccagt gaggacatag tcgcctaggg aaatctcatc tgttaccaag
     9301 gtcttaatgc gctcatcata accctcatag tgcccacaga taaagattag ctcttcctct
     9361 tgagccaaat cttcagcata agcctgatca aactgctttc cagcaggatc gaggagaata
     9421 acgcgcggat ttttcttttc aatagcatca aaggaatcga aaataggttg tactctgagc
     9481 aacatgccct gaccgcctcc gtagggctca tcatctacat gacgggcctt ttcagcattt
     9541 tctcgaaaat tatgatactg gatatccaag agcccttttt ctcgagcctt tccaacgatt
     9601 gagtgctcca gtggagaaaa catctctgga aagagggtta aaatatcaat cttcatcgtc
     9661 taacccttct aagatttcca catcgacccg tttatttgga atatcaacat tgagaaccac
     9721 tggtgggata taaggtaaaa gcaaatcacg tttgcctttt cgtttgacca cccagacatc
     9781 attagcacct ggttgcagga tttccttgat ggttccaacc aagctatcac cctcatagac
     9841 ttccaaaccg ataatctcgt gatagtaaaa ttcaccatcg tctaggtcat tcaaatcttc
     9901 ctcagcgacc ttgagactgt atcccttgta cttttcgata gtattgatat ggtacatatc
     9961 tttgaattta ataatgtcaa agttcttctg tttacggtgg ctagcgatgg tcactgtttg
    10021 gacaaactga tctttttcat caaacaaagc cagctcagct ccttttttaa accgttcttc
    10081 tgcaaaatcc gtcacagaca agactcgcat ctcaccctgt aatccctgcg tattaacgat
    10141 tttcccaaca ttaaagtagt tcatcttgtc tcctgtaatc tccttttttc catcttattc
    10201 tgacaattct cgaataatag ccgcaatttt ttccgattct gaccattgta aataatggtg
    10261 attccttcct aaaatgagtt tagtattgga agtccaatat tctgattctc tgtactcttt
    10321 ttctctataa gtctgacaaa aaataaatac agggacatga gattctatcg acaaattctc
    10381 aaaatcttcc tcagtaatct ctccagatat ctgaaattct ggatcttgat tttccaactc
    10441 taagcctttt tcttgcatta attcccagat ttttttattc gtttcaggac taaatgttgc
    10501 ttgagttaag ttcttaaaat aaagttcagg accacactcg tcaatcagcc tcatctactc
    10561 ttccatttct ggataaggat tttctgaaaa atcagcaaac atgacttttt tagttgtcgg
    10621 ttcaattgct actaaagtct gacgcttaat tggtttctcg agtaatttgc aagctaaaat
    10681 tccactccaa ctatgtgcac aaagtatata ttcagaaatt cctaattctt caagtacttc
    10741 ataaaccgca tctgcaagat tatctagttt ttttccagct tggtcatgaa tcggactcct
    10801 acctgtgttc ggaaaatcaa ttgtcaaata accaattgta ggaggaggtt tttcaagtat
    10861 aagtgaaaaa ttttcataac ttggtagcaa acctgctccg tttagacaaa ctagcacttt
    10921 cttttgcttt tgataagtaa cagagaggct accaatttct gtagatactt caaacctctt
    10981 cataaagaaa tccactgatt ctatataatg aattattaaa aatccttatc ctttatttta
    11041 tcacgttcca aggattttct caagttggag gaaggggaca acatctctac tttcccttca
    11101 agaatccttc caaattatgt ttatgttggt aattaacggc tgcggttttg tctttctcaa
    11161 agacagtctt ggtaaggtca atatgattaa tagctacgat tgcgacggtg tagtaaatga
    11221 tatcagccag ttctctggca agttcctcgt tcgaatccta tcccttcttt tcgaccagag
    11281 cgcctattca aaacctcggc tacttctccg acttcctcca ctaacttcat aaagagacct
    11341 tcatcagtcc gagactgctg ttaatgttcg attaagtagt cttggaattg cctaaacgtt
    11401 caatctttta tagtatattg aaactagaat agtacacctt tacttctaaa acattgttag
    11461 aaatcgattt gactgtcctg atcgatttgt cctgttcttg tttcatttta ctatatcttc
    11521 tattccacac aaaaaagcga gacatccgtc ccgcccttct tatttttcgt caataacgat
    11581 tcttactttt ttgtattcag ttgggacaga gtagacaatc gttcttatcg cagaaatagt
    11641 gcgaccctta cgaccgatta cacgacccac atcgctttga tcaagattca aatgatattc
    11701 caaaaattct ggtgtatcct caatcttgat agttaaggca tctggttgtg aaattaaggg
    11761 tttcacaatc gcaataatga gattttcaat cgtatccatc tgtcaaccta ctttaaactt
    11821 attttgaaaa tttagaatcg tggaattttt tcaatacgcc ttcttttgaa aggatgttac
    11881 gtactgtgtc tgaaggttga gctccattag ccaaccatgc aagaacgcgg tcttctttca
    11941 aagttacttg gttttcagca acaagtgggt tgtaagttcc aactgtttcg atgaaacgtc
    12001 cgtcacgtgg tgaacgtgaa tctgctacgt tgatacggta gaaaggtttt ttcttagaac
    12061 ccatacgagt caaacggatt ttaactgcca tttttaaagt ctcatttctt taatttttta
    12121 tttcggtgaa atagctgagc tatttagcac atgttctatt atagcagatt tctggcaggt
    12181 gtcaagaaaa aaacttgaca gactataaat ttcttaaagt atttagaaat ttgttagaat
    12241 aggaaataaa tgtttgaaag ctgctatccg tgaatactat tttagaaaaa ttttataaag
    12301 aacaccaagt caaacccttc atatcgcctg aacgagattt ggatacttgg ctcctaagtc
    12361 ccaagcccgt tcccaagcga aatatggact tattagcaga tgattcacta gcaggagata
    12421 tcattttact atggagaatc cagtttggta cttttaccac tgaaacatga ttttaaaaac
    12481 tcccattcga atatgattca aaattcgctt agaatcaatg ttttcccact ttttcaaaaa
    12541 ataaattttc atctaattta aataactttc aattaagtgt gaattttgct taaaatcatt
    12601 aaaaaacacc ccatggtgtg aaccatgaag tgttgcaaat gctgtgttgt agctagatct
    12661 taacgtttag agaactggct agctttacga gctttcttaa gatctggttt ccaaaatact
    12721 ttgagtacac ttatggtatt gatgccccta aacatctaaa aactctggta gaaaaagggt
    12781 atgctcttgt cgaaacttct tttgactcac ttgaccatct aaatactacc ctgaaaaaga
    12841 atatcctgaa gaagaaggga gttactggac tttctaaaat gaaatcttgc gacctggatc
    12901 aagcccttca tgagcatttt tcagaagaag aattagctgg tcactttcat gtccttctat
    12961 ggactttttt acaatggcat tgctatcaca cccaatacct atctaagcgc ctggttcgta
    13021 aactttattg cagctcttcc tctaaatttc ctaattgttg aaccaattgc ccgttttata
    13081 ctaagttctt ttcagaaacc atttactggg gaagaagttg aagattttca agatgatgac
    13141 gaaatcccaa ctattatcta atccagttct gtaaactact aatatttgaa atccacttcc
    13201 ttttagggta caatggtata aatgaatttt tgagaggatc agaatgaaaa aactagcaac
    13261 ccttctttta ctgtctactg tagccctagc tgggtgtagc agcgtccaac gcagtctgcg
    13321 tggtgatgat tatgttgatt ccagtcttgc tgctgaagaa agttccaaag tagctgccca
    13381 atctgccaag gagttaaacg atgctttaac aaacgaaaac gccaatttcc cacaactatc
    13441 taaggaagtt gctgaagatg aagccgaagt gattctccac acaagccaag gtgatattcg
    13501 cattaaactc ttccctaaac tcgctcctct agcggttgaa aatttcctca ctcacgccaa
    13561 agaaggctac tataacggta ttaccttcca ccgtgtcatc gatggcttta tggtccaaac
    13621 tggagatcca aaaggggacg gtacaggtgg tcagtccatc tggcatgaca aggataagac
    13681 taaagacaaa ggaactggtt tcaagaacga gattactcct tatttgtata acatccgtgg
    13741 tgctcttgct atggctaata ctggtcaacc aaacaccaat ggcagccagt tcttcatcaa
    13801 ccaaaactct acagatacct cttctaaact ccctacaagc aagtatccac agaaaattat
    13861 cgaagcctac aaagaaggtg gaaaccctag tctagatggc aaacacccag tctttggtca
    13921 agtgattgac ggtatggatg ttgtggataa gattgctaaa gccgaaaaag atgaaaaaga
    13981 caagccaact actgctatca caatcaacag catcgaagtg gtgaaagact acgattttaa
    14041 atcttaaaaa ccaaaaaaaa tacagtatcc acattcggta ctgtatttct tttactctca
    14101 ttcttaagtt aaattattaa aatcccatat ttggtctatc caaccttcat aaaagtctgg
    14161 ctcatggcag accataagga tagatcccct gtattctttg agagcgcgtt tgagctcatc
    14221 ctttgcatcc acatccaaat ggttggtcgg ctcgtccagc actaaaacgt tgttttcacg
    14281 attcatcaag agacagaaac gaaccttggc ttgctctccc cctgataata cttgaatctg
    14341 gctttcaata tgtttggttg tcaaaccaca acgggcaagg gctgcacgga cttctgcttg
    14401 attaagggca ggaaaggcat tccagacagc ttcaagagga gtttggcgat taccaccttc
    14461 tacttcctgc tcaaaataac caagttctaa ataatctcca cgctccactt ccccagcgat
    14521 tggcgagata atgcccaaga gactcttcaa gagagttgtt tttccaatac cattagcacc
    14581 aataatcgca accttttgat tgcgttcgaa ggtaagattt aaaggcttag taagaggacg
    14641 gtcgtaacca atttgcaagt tcttggcttg gaagataaag cgccctggtg tacgagctgg
    14701 tttgaaatca aaagatggtt ttggtttctc actttggagt tcgataatat ccatcttatc
    14761 caatttcttt tgacgagaca tagccatatt acgagttgca acacgggctt tattacgagc
    14821 cacaaagtcc ttgaggtctg caatctcttt ctgctggcgt tcgtaggctg cctctagctg
    14881 agatttcttc atagcataaa cttcttggaa ctggtagtag tcaccagagt aacgcgtcag
    14941 ctgttgattt tccacatgat agacaatatt gataacgtca ttgaggaatg gaatatcgtg
    15001 cgaaatgaga acaaaggcat tctcatagtt ttggagatag cgcttgagcc aatcaatatg
    15061 ctcagcatcc aagtagttgg tcggctcgtc caacagcaaa atatcaggct tttcaaggag
    15121 aagttttgcc aaaagcacct tggttctttg cccacctgac aaagaagtta catccgtatc
    15181 catgccaaag tccataacac caagagcacg cgctacttcg tcaatcttag catccaaggt
    15241 atagaaatca cgactctcca gacggtcttg aagttctcct acttcttcca tgagagcatc
    15301 aacatccgcg ccgtcttcag ccattttcat atagaggtca ttgatacgag cttcagcttt
    15361 gaaaagctca tcaaaagccg tacggagaac atcacgcacc gactgtcttt cagcaaggac
    15421 agagtgctga tccaagtaac cagccgtcac atatttggac cactcaacct ttccttcatc
    15481 tggcagcatt ttaccagtca cgatactcat aaaggttgat tttccttcac cattggcacc
    15541 gaccaggccg atatgttctc ccttgaggag acggaaggac acatcttcaa aaattgcacg
    15601 gtcaccaaaa ccgtgactca gatttttaac ttctaaaata ctcattttaa ttccttatct
    15661 tgtttttatg taatcgttta taaaggagcc aagccagata gccacccaaa gtgttggtcc
    15721 acaaatcatc aatctcaaag acacgattga aatcaaagaa aaagtccaag attaattgcg
    15781 tacactcgat tccaagactc acaagaaaac taaaaagaag gacctttttt gttttccgca
    15841 aatttggaaa tagataaagg agttggaaaa tcagaggaaa aaacaagaag acattgagga
    15901 tattttgtaa aaaaatccaa cataattgtc caatgtcact cacttcgccc agtttccaga
    15961 gagaattgaa aggagtcaaa agaaaaacca ggcgtccaag atgctgaata tctggagttc
    16021 ccactcccac ggtagattgt tcttgaggag taaagcaaaa acagacaatg caaatgctat
    16081 agaaaatgac tccccagacc aaaatatgat tataagtctt cttcatcatt aaggatttac
    16141 cgctgcgact gccttctggc ggtcacgttt cattgtgtta gagcgcaatt gtccacaagc
    16201 tgcgtcaata tctgtaccat gctcttgacg aaccacacag ttgacccctt ttttcttaag
    16261 cgtatcatag aaagccaaca cgcgctcttt gggactacgg ctatattggt catgctcact
    16321 aactgggtta taaggaatca agtttgcata agacaatttc ttgatgttct tgagcaattc
    16381 agtcaattcc aaggcttgtt ctacactgtc gttgacttca ttaagcatga tatattcaaa
    16441 ggttacacga cggtttgttg tctcaatgta gtattcaata gcagcaaaga gtttttcaat
    16501 cggaaaggca cggttaatct tcatgatact tgaacgaagt tcattgttgg gtgcgtgaag
    16561 agacacggca agattgacct gaaccccttc atcagcaaag tcacgaattt tatgagccaa
    16621 acctgaggtt gaaaccgtga tgtgacgagc accgatagcc attcctttat catcattgat
    16681 agtacgaaag aaattcaaga cattgttgta attatcaaag ggctcaccga ttcccatgac
    16741 aacgatatgg ctgacgcgtt catcctgacc acgctcatca aagtatttct gaaccagcat
    16801 gatttgcgct acgatttcac cgttattgag gtcacgttgc ttcttaatca aaccagaggc
    16861 acagaaggta caaccgatat tacagccgac ctgagtggtc acacagacag ataaaccata
    16921 gtgttgacgc atgagtacag tctcaattaa cataccgtcg ggcaattcaa agagatattt
    16981 gactgtacca tcagcagact cttgcacaat accttgtttc aagggattga ccacaaactg
    17041 gtcattgagc ttagcaatca aatccttgga aaggttggtc atttcttcaa atgactgcac
    17101 acgtttacgg tagagccatt cccagatttg atctgcacgg aatttctttt ctccctgctc
    17161 caatacccat tcctgcatgg tttgatgtac caaactatga attgagggtt tcatttcttc
    17221 tccttattct ctactcactt ctgacgaatg acaaaatgac gttgtccctt gtcctctttc
    17281 tgagaatgct ggtctttctg acgacgtcta ttcttcttat ctgcattcga ctttcgttta
    17341 gtttgagtcg gtttctttcc ttttctagaa ggtgtttctt cttccgtctt acgcattttc
    17401 ttgtcaaatg atgctcgctt aggggcttca ttttctaaga caaaataggc acaaccataa
    17461 ctacaatact ctaaaaggta gtcttgtaaa cgactgattt tttcaagttt ttcttctgtt
    17521 cggtcatcct tgtaaaaacc tcgtaggcga agctgttcgt tgctccagtc ccccacgata
    17581 taatcaaact tggttaatac ttctgaaaaa cgctgattaa aagtcgtcac atcaaaggca
    17641 tccttgatat tttcaaccaa ggaaaaagct atcccttccg tttcgacctt gtccccgtgt
    17701 aaatggaact ccggaccagg aaacttgtta tagttgtata attcaggtgc aatttctttt
    17761 cgcatagata tcctttttcc acgattactt aatactttat tctaccataa tttctagcag
    17821 ttagcacgtt tctcataaaa atgaaaaaag tctgacgatt ttgtcagacc agaatcttat
    17881 aacctaaaaa agagaagaac aattcttccc tccaactatc attatttagc agctgcgtac
    17941 aattcatcta ctttattcca gttgattact gaaaagaaag ctttgatata gtcaggacgc
    18001 acgttgcggt atttcacgta gtaagcatgt tcccaaacgt ccaagcccaa gattggtttt
    18061 ttaccttctg agattggtgt gtcttggttt gctgttgaag tcacttcaag tttcccttct
    18121 ttgttgacaa ccaaccatgc ccaacctgaa ccaaaacgag ttgttgctgc tgcagtgaag
    18181 gctgcttgga attcttcaaa tgaaccaaat gttgcatcga ttgctgctgc cagttctgct
    18241 gaaggagctg ttttctcggg agtcatcaat tcccagaaaa gagcgtggtt caagtgtccg
    18301 ccaccattgt tgataagtgc ttgacggata tcagctggga tagattctac atcagcaagc
    18361 aaggcttcaa ggtcttcacc gatttcaggg tgtttttcta aagctgcatt ggcattgttg
    18421 acataagttt gatggtgttt gtcatggtgc aaatgcattg tttccgcatc gatgtatggt
    18481 tccaaagcgt cgtatgcata tggaagttct ggtaagataa tagccatctg taatacctct
    18541 ttttctttct atatgaaaat gataacgcaa acattcactt ttttcaagtt ttttgcttat
    18601 agataaggaa atcactgaca tactttctaa taaaataagc aaatcagcaa gaaacccacg
    18661 acaagataat cctgacgcgg gttgtagttt caacaaatgt caattgacct gactagcaat
    18721 ctgtaagagt gccttttcaa aaaggaaacc tttttcataa agacctgtct taatctgata
    18781 gtctgtctca atcaaatagg aaatagcttg cttcagaaag ctcaaagaaa gtcctctcga
    18841 atctcttaat gcaaacttga tttgataagg atttgggtta cgtcccagat aactacctaa
    18901 actacttgca atctgcgatt ctgtttggcc agactccgcc aaaatcttca cctgagtaaa
    18961 agtccgaaat tgtcccagca tgactgcaat cagtttgatt tcatcttccc cttgcaaggt
    19021 caagtctctc accaaatcgc gcgcctgatc catcttttta gtcagaataa actgagttaa
    19081 atcaaaaata ttgtcctgca aggtcttggg aattgcgtta acaatatcct cttcctcgat
    19141 aacagaatcc gccttatagg actgtaaaaa gagaagattt ttctggattt cgctaaattg
    19201 aaaccccgac ttgatgagga gattttcaaa agaatgattg gtaaactgca gaccttgttt
    19261 ctgactccac ttttggaaat actggcgcaa ttcttgttct tttacttcta ctgcatcgaa
    19321 gaccttggca tcacgcttaa gtaatttgac taaccgtctt ttgctatcca gctttccttc
    19381 tgcaaagatt atcaacttag ttgttggaga aggattgtca aggtattcct caaatgactt
    19441 aagctcatca tctgtcaaaa agcgtttctt agcagtcgtg atatccataa aataatctaa
    19501 tatcacgatt ttctcatccg caaagaaagg aaggctgacc aactccagtt ccacatcctt
    19561 gtaaactact tctttcatat caaagtaggc aaagttgagg tcagcagaat catacccaat
    19621 ctgtttcaac atttgactct tcatcacttc aaactgaccc tgatctgtcc ctgtaaatag
    19681 gctcaggctc ggtaaatttg ataaagtcaa cttctgactt tcttcaatgg ctagcatcga
    19741 ctctcctttc ttcagatttt tcgatttaat ttagtcaata tagcgcaatt tcccacggaa
    19801 atcttctaag ctctcgtagc ctttttccac catgattgct ttcagttcat tggtaatgcg
    19861 gtcaaaagca ctgacgcctt ctttgtgaag ggtcgttccc acctgcacca tacttgctcc
    19921 acagaggatg tgttcaaagg catctcgacc agtcagaacg ccacctgttc cgataatttg
    19981 gatttgagga tttaaacgtt gataaaaggc gtgaacattg gctagagcag tcggtttgat
    20041 gtattctcca ccaattccac caaaaccatt cttaggccga ataacgacag attcgtcttc
    20101 tatatagagg ccgtttccga tagagttaac gcagttgaca aacttgagcg gatatttgtt
    20161 gaaaatagct gccgcttgat caaagtgaac aatatcaaaa taaggtggca atttaattcc
    20221 aagaggtttg gtgaagtaag caaacacttc tgccaaaatc cggtctgttg tctcaaaatc
    20281 ataggcaatc tgaggtttac ctggaacatt tggacaggaa agatttagct cagtcagacc
    20341 acgaaaatca ctctcttgga cttttttcaa aatagtatgg gtttcctctg gagacatgcc
    20401 gaccagagat aagaagaaag ttcggttcga ctctttttcc tgcaaatcta aaagataatc
    20461 caaataatag tctaagccat tatttggcaa gcccatagag ttgatggaac caagtggaac
    20521 atcttggtag cgtggctcag gattcccctg acggaagtcc aaggtcgctg tcttagtaac
    20581 aaaggttcct gccgctgagt ttttgacctc ttctaactcc tctatcgtca tacaagccac
    20641 acctgctgca ttcatcaagc aattgtcaaa ctcaaaacca gcaatttgtg ttttcgttga
    20701 taccatgatt ctaatcctcc agatttcttt tattgctaat attataccat aattttaatt
    20761 ctaggtttac tagattctgt ggttctatct atttattcgg aaaagaaagt ctagtacaga
    20821 agtcatgcta tggaaacagc tagtgtatta gaagtttgaa caaggcttgg gtaacagact
    20881 tttgaatgaa gtccaagcaa caaggagaaa tctcaaccat gagtccattc catgactctg
    20941 ttgctctaga aaaccttgca atagtcaagt gataacgaat aaaactttgg attgaaatat
    21001 ccccaagatt atctattacg aggagaaatc ttaaccttta tcatttttaa agaaatatag
    21061 taaaatgaaa caagaacagg agaaatcgat caggacagtc aaattgattg ctaacaatgt
    21121 tttagaagtt gaggtgtact attctagttt caatctacta tacatgtaaa tctctacttt
    21181 cgcaaaatgg attttcagaa cagtatccca gtatagttaa aaactataaa aaagagagga
    21241 cgttcccctc ttaaaatctt attttactgc ttctttcaaa gcatctacct tatccaaacg
    21301 ttcccatgga agatcaatat ctgtacgtcc catgtgaccg taagccgatg tttgacggta
    21361 aattggacgc ttgaggtcca gcatttggat aatccctgca gggcgaaggt caaagatttg
    21421 acgagccgct ttttcaagtt gactttcagc tactgttcct gtaccgaaag tatcgatacg
    21481 aacagaaaca ggttgcgcaa caccgatagc ataggccaac tgcacttctg ccttcttagc
    21541 aaggcctgct gcaacgatat tcttggcaat atagcgagcc gcataagagg ctgaacgatc
    21601 caccttagtc gcatctttac cagagaaggc accaccacca tgacgagagt agccaccata
    21661 agtatctaca ataatcttac gaccagtcaa acctgagtcc ccttgaggac caccgattac
    21721 aaaacgacct gtcggattga taaagaattt tgtcttatca tcaagataag aagatggaat
    21781 aacttctttg atgaccttgt caatcacatc ttgatggatt tgttcattag tggcctctgg
    21841 atcatgctga gtagaaataa cgactgtatc tacacgtacc ggacggtcat tttcatcgta
    21901 ctcaactgta acttgtgatt ttgcatctgg acggagatag ctaatttctc cagacttacg
    21961 aagttctgcc agacgacgaa ccaatttatg actgagtgca attggcaatg gcataagctc
    22021 ttctgtttca tctactgcaa atccaaacat gagcccttgg tcacctgctc caatcaagtc
    22081 cagtggatct tgatcagcat ttccacgaac ctccaaggct tcgttaacac cttgagcgat
    22141 gtcaggagat tgttccacca aagatgggtg tactcccacc gtctcagcag aaaatccata
    22201 ttctgtattg gtataaccaa tctctgcaat ggtatcacga accacacggt taatatccac
    22261 ataggcattt gtagaaattt caccaaaaac gtggacagaa ccagtatata cagctgtttc
    22321 agcagcaacg tgcgcctctg gatcctttgc taaaatagca tccaaaatcg catctgaaat
    22381 ttggtctgca atcttatccg gatgcccctc agatacagat tcagacgtga ataatttacg
    22441 ttctgacata aaaatgtccc cccttaaaaa atattgttat agagttacaa agtactgata
    22501 ggaaaactct tcgtaataaa tacctaccat cttatcggga ctatataact ttatcattat
    22561 accattttta atcgcaattt gcagtcttta atgtagtgtt tcggtttaga tagttcgttc
    22621 tcataaagca aaaagttgga gctcagtact ccaacaattt ctattaaaat gtttgacgtt
    22681 tagctttacg ctctgcacga ccgcgagcgc gattttcagc acgcttggtt ttacggcgct
    22741 tttcatcaac cgcccattga attttcttct tataacccgg tttgactttt ttctttttct
    22801 ttttaaccaa accaatcatt tcgatatcaa gtttatcttg ttttttctca cggttggcac
    22861 gacgatcacg gtcataggta tcttgaaatt ccccgtcttt gaccatctta ggactaaact
    22921 tgattcccaa tttctccaac tcacggatat ccgagtcatc acttggctga taaagggtaa
    22981 tagctgtacc tggtaggcca tttcgtccag tacgaccaac acgatgaaca aaaaaagata
    23041 agtcttgcgg aatggcatca ttgatgacat ggctgacacc ttcaatgtca atcccacgcg
    23101 ctgctaaatc tgttgcgaca atatactcaa aatccagatt ttgcacctga ttcatgattc
    23161 gcttgcgttc acgaggggca atatcgccat ggatttttgc aaccttcaaa ccttgagaag
    23221 tcagatatga atgcaattca tcagcacgcg ttttagtgtt aacaaaaatc attgccaaat
    23281 acggctgcat caactgagtc aaccagtaaa tttgagcatt cttatcatgt cccttggtcg
    23341 aaatcaacca attatcaatg gtgtcagaaa taaccgtttt agtcttaatt ttctccataa
    23401 caggatttga taagtatttt ttcaagaatg gttgcagttt ttgtgggata gtcgctgaga
    23461 agaccatgaa ttgcaagtct tttggaagac tgccagcaat cttatcaaca gtttccaaga
    23521 atcccatatc caaggtcata tctgcttcat caacaacaaa tgtcttggct ttatgaatag
    23581 ctaaatcacc agatttaacc aagtcgtaga tacggcctgg tgttccaata acaatatgag
    23641 gctgattgct tgccaatttc tcaatctggc gagccttatc cgtaccaccc acataattaa
    23701 ccacacgaac ttcgacatct gagtgagctg aaatctgacg cgctacttgg taaatttgag
    23761 tagccaactc acgactcggt gcagtaatca ctgcttgtac actatcgcta gcttcatcta
    23821 attgctggaa aatcggtaac aagaaagtat gagtcttacc tgaacctgtt tttgattctc
    23881 ctactaggtc acgacctgcc aaaacaatag gaatcaactt gtcttgcacc tctgttggag
    23941 ttgtaaattt taactccttc aaggcttctc taatatagtt tttaaattga aatttcgtaa
    24001 atgacataac atcctcgatt ctatctatct tatcaattat accatatttt attccattac
    24061 agtagtctca cttatttagg ctatttccag tagcttctct agtaagaaaa ggctggaatt
    24121 ttatagttcc aacctctttt cagttattat ttccagttta atatagcatt caagccatag
    24181 tgatcactca cttgtggact cttgttacca tcaaatacga catgtaaatt ttccaccgct
    24241 aactctttgg tagtaaagac ataatcgatt cgaaggggtt cagtgttccc tttccagcca
    24301 tcaatttcag gcggaacagt atagctacca cttttctctt gagcaacttc aaatgcgtct
    24361 tgtaagccta atggactagc taaaatagct tggtaacctt cctgacctgc tgggttgtta
    24421 aaatctccag ctagtaaaag tggcttgttc aattttttca agacagcctc aaatcgtgcc
    24481 cattcttctt ggaaaccttt atcccaccaa gagagatgaa cactggcaac tgctagctcc
    24541 ttgccatcga ctacagtttc agctagggca acacggcgag tatgatagtc tgttggatca
    24601 tccacatctg aaaccaaaat ttctctggct tcaataggtg ttttagacaa gatagccaca
    24661 ccttcgtggt agcggtcata gccgatatgg ttataagccc aggtccagta gtaatttttc
    24721 ccttgctcta acaacttttc aaccaagagt ctaacataat ggtcttggtg aataggctca
    24781 gctgctggca aagcttgata aaggtcatta acctccacct ctgacgaggt catctcctga
    24841 ttgatttctt ggaaacaaat caaatcatag tccttttcaa gaatatcttc aagcaaaatc
    24901 tggaattttt cctctgcttc tttctccatc caactgtgag tattgagtgt taaaaatttc
    24961 attcttttct cccaaaataa gaggttggaa tacagcttcc aacctcaagt atattacaat
    25021 tctactttag caactgctgt tttagctgca agagaacctg ttttttctaa tttaactgat
    25081 ttaattgcat caccatttgt gaagacaact actgttgaag tttcacgtcc tgctgcacgg
    25141 atagcatcca agtcagctgt gacaaggaga tctcctgctg caactttttg tccttcagca
    25201 acatgaactg taaatggttt accttcaaga cttactgtgt ccaaaccaat gtgaaccaat
    25261 acttcaagac ctgcttccgt cacaatacca aaagcatgtt ttgttgggaa gatgcttgac
    25321 acagtacctg aaactggaga tacaatgttt ccatttgcag gttctactgc aaatccatca
    25381 cccatcattt tttgagcaaa tactggatcc tttacttgtt ccaaaccaac aacttgaccg
    25441 tctgctactg agtaaacttc ctcagtaaga tctttgaagt gaacagtgtt ttgttgtgct
    25501 tcagtcattt ggcttggaag agtttcagga atgatttcac ctgaatcaag gatatcttgg
    25561 atatcagatt tcaaaatgtc agcttttgga ccgtagatag cttgaacccc ttgtcctttc
    25621 atcacaagac ccatagctcc ttctgctttc cattgctctg catttcctac tttatctgca
    25681 tctttaacag ttacacgaag acgagtcata catgcatcaa catcaacgat gtttacacgt
    25741 ccaccaagaa ggttgataat gtttacagct tgagagcctg ctgcaacttt cacttcgctg
    25801 ctggtttctt ctgaaccttc agcagtttcg tagtttccgt tgcgccctgg agttgcgtag
    25861 ttgaattttt gaatcatgaa gtttgcgata aagtacatga ttacagcaaa gagaacagtt
    25921 acccaaacga agttaacgat atccatacca ataccagcac tgattgcaat aggtgtacga
    25981 gtcaagaact cgattgaacc gaatgagtgc atacgtaggt ttacgacgtc agccatagcg
    26041 aaggcagcac cttgaacaag tgagtaaaca agatacatag gtgttgcgat gaacatgaac
    26101 atgtattcga ttggttcagt aacccctgtc aagaatgttg caagagctgt tgcaatcatc
    26161 atacctttgt atttatgttt cttgtcagca tcaacattac ggtagatagc aacaatcaca
    26221 cccatcaaga taccgaatga accgatcatt tgtccaactt tgaaacgagc tggatgtact
    26281 gtatctaaca agtgttgata ttgactagca tcagtacctt taaggtttac aaggtctgtt
    26341 acccatgcaa gccatagtgg gtcttgaccg aatacttgag tacctttagc tgcaccagtt
    26401 aaaatgtcat aagtaccacc aagagctgtg tagttcattg ggatagtcaa catgtggtga
    26461 agaccaaatg gcaagagcaa acgttccaaa gtaccataca agaatggtgc aagaattgga
    26521 gcagtttctt gtgagttggc aatccagata ccgaagttat tgatacctgt ttgaactact
    26581 ggccagaaag cagcaagtag aattgcagcg attgctgaac gaagaataac tacaaatggt
    26641 acgaaacgtt tcccgttgaa gaatgaaagt gcatcaggaa gtttacggaa gttgtagtat
    26701 ttgttgtaag cagttgcccc tacaaaacct gagataatcc ctacgaatac ccccatgttc
    26761 aaggctggag cttcaagaac actgataaag taatcagcaa ctttgattga accaccaaag
    26821 aaagtagtta ccatagcatc tggatttttc aacatatcgc ctgatacacc aaagattgta
    26881 ccagtgatac ggttaatcaa gatgaaggca agaccagcgg cgaaagcacc accagcacgt
    26941 tctttagccc agcttcctcc aatggctagg gcaaacaaaa tgtgaaggtt accgataacc
    27001 ccccaaccga tttgctcaag aattccacct gtgatgacaa gtggtgtaaa ggttgggtta
    27061 atcatcacga tagacttacc gattgaaatc atcaaaccag cagccggcat aaccgcgata
    27121 actaccatca aagccttacc gaatttttgc caaaattcga aagacaagac atttttgaat
    27181 gtatctttca tcattcaaat ctcctttatt tttatttagg caaacgtttt acgaaaacgt
    27241 ttgcaccttc tataatttaa ttataccact ttaatttttt ttgtaaaggc ttttataaaa
    27301 aaattacact tttttctaaa aaatttctag tgagtcaaat ttcaagttca aattagccat
    27361 caagaagtct tctctagagg acttctttgg aagctctttt cttctacgca tctcttttca
    27421 aaaactcttg tagcaaaaaa ggagatttta caatctcctc ataaaagcag ctattttacc
    27481 taaatcttca agaatcggcg catggatatt cctgatccca atgaaccaat gaaaacccca
    27541 atcacaaata gtagggcaat catcaacgga ctaaataaat ctggactaat catggataga
    27601 ttttgcccta ccaacgattt gttgacagat tggtaaacaa tttgataaac aataaagacc
    27661 aaaacagatg gtgcgatagc tcccaataaa ccgataaagg ctccttctaa caagaacggt
    27721 ccacggatat aactgttttt agctccgacc aagcgcatga tttgaatttc gcgactgcgg
    27781 gaaataatgg taatacgaat ggtatttgaa atcaagaaaa ctgcgataaa aattaacaaa
    27841 gcagcaatcc ctagtcccca aacacggata aatgaagcta acttgaagag tctttctgta
    27901 ttggcaccgc catcttgaac ctcagagaca ccttcaattt ttttagcatc ttcggctata
    27961 gtttttacat catttggagt gtttgcctct acaatatagg catcatagag aggattggca
    28021 tctccttcaa agattttcca gttatctccc attatctcgg ttaatttttc atattgttct
    28081 tctttacttg aaaaggtaac acttttaacc gtagacatgt tcttcaaaga atcatatacc
    28141 ttgtggtagt cattatttgt aacagtttga ccttcttttt caattgtctg actattatct
    28201 tccacatcct ttcggatata aactactaca cggacattat tttcaatatc tgtagctagt
    28261 ttcgctgtat tgaaaataac agatgcaaat attgccacca aggtcaaagt aatcatgact
    28321 gaactgacag cagctactgt catccaacca tttcgtttca aactttttaa ggcttcaaat
    28381 aaatggcgaa aaaatctact aatcatcgta tccatactct ccttttgatt cgtcacgaac
    28441 gacacggcca ttttcaatgg caatgacacg gtggcgcaag gtatttacaa tctggctatt
    28501 atgagtcgcc atcaaaatag ttgttccttg taggttaatc cgttccaaga gattcataat
    28561 ttcccatgaa ttatccggat ccagatttcc tgttggctca tcagctatca atactttggg
    28621 attatttaca attgcacgcg caatcgcaat ccgctgttgc tccccacctg agagttcatt
    28681 tgggaaagaa cgaaccttat gcttcaatcc aaccaagtcc aaaacttcca tcactcgtct
    28741 tttgatatta cggcgatttt ccccgattac ttccatagcg taagcaatat tttcatagac
    28801 agttttcttt ggtaacaatt tataatcttg gaagacaacc ccaacactac gacgtagaag
    28861 cgggacatct ttctttttga tcttaaccag attaaaacca gcaactgata gacttccttt
    28921 atcgattttt acttcacgat acagagaacg aataaaagtt gacttccctg ctcctgaagg
    28981 tcctacgatg taagcaaatt cccccggttg aacgctaacc gaaacaccgc gtagagcagt
    29041 cgttccgttg tcgtattttt taacgacatc tctcatttca ataattgaca tgtgagttcc
    29101 tttctatctt agctaattcg ccacttgaga taagcatcga taaaaccatc taggtctcca
    29161 tccataacct tatctacctg agcaacctca tagctagttc ggtgatcttt taccatagta
    29221 taaggcgtga agacataaga acggatttga cttccccaag tgatctcctt tttctcacct
    29281 ttgagagaat ctacctccgc agccttcttc tcttgctcca tttgatagag cttagcctgc
    29341 aacatcttca tggcgcgatc tctatttcca tactgggtac gatccactgt tgattggaca
    29401 acaattccag ttggaatgta ggttaaacgt acacctgttg aaaccttatt gacgttttgt
    29461 ccaccggcac cacctgaacg gaaggtatcc atcttgatat catcttcacg gatttccact
    29521 tcaatagtat catccaattc tggcatcact tctacagatg tgaaagaggt atggcgacgt
    29581 ttggcagagt caaatggtga gattcgcact aagcggtgaa cacccatttc tgacttgagg
    29641 agaccatagg cattaggccc ttcaaatgat aaagttaccg acttaatacc agcctcatca
    29701 cctgcttggt aatccaacac ttccacttta aagcctttag cattaccata acgagtatac
    29761 atacgaagca acatatcacc ccagtcctgc gcctcagtac caccagaacc tggatggatt
    29821 tccaagatgg cattgttgtg gtcataaggt tctgacaaga gtagagtcat ctcgtagctg
    29881 gtcattatct tatcaagttc ggctaactgc gctaccagtt catcatgcac tgactcgtct
    29941 tcagccaaaa aatccaataa aatttcgact tcatcctgca actcttccat cttatggaag
    30001 gtattgtaag tgttttttaa ttcatttaat tcttgcgaca ttttttgggc cgcaatatta
    30061 tcgttccaaa aatcaggttc tgtcatcttg ttttccaaga tggcaatctc ttcctctagc
    30121 ccttcgaggt caaagagacc ccctgaaaga agctaatttt tcacgatttg cgtcaatttt
    30181 ctgacgaatt actgaaatgt ccataaatac tcctttttta cattatttca ttatagcata
    30241 ttttatcatt tctttccatc ttttgctcta atcccctttt catgaaaaaa gaagcctttg
    30301 ggcttccctt cttacaagac ttttgctgaa gtgcgaacga tttcttccac ttgaatacca
    30361 tttgcttcaa atttttcttt caaggctggt aaatcaattg atccatcgat ttgcacttcg
    30421 ataatcacct taccatcctt acgcggaata ttgactgtat gggagatatt caaattttct
    30481 tctacaatca aagaaacaat ttttccaaga acaccaactt catcttctgt aacaaagcgt
    30541 acacgaatcc cttcttcgcc ataacctgca atttcaagaa aggcttggaa aacgtcacgg
    30601 tcagtaataa ctccgtatac ttgatggtta tctacgacag ggagaatacc aatcttattt
    30661 ttcaacatca gataagttgc atcttctaga ctagcatagc ctgagacagt gacaacatcg
    30721 cgaatcatga catcttttac ttttgtctta ttcagaagat aattcatctc atagatagaa
    30781 agacttgttg ctttagatgg acttgcttgt gcaatggttc cctcagtcac caaaccaacc
    30841 aattgatcat tttcgataac aggcaagcga tgcaattctt gctctctcat caaatctgct
    30901 gcatgagata ctgttgtatc tggactaata taaactacct tgcgggtcat aaaatcttta
    30961 actgccatga gacttctcct tttctattct tatccccatt atacaatctt tttgtaaatc
    31021 tatcaaacgc ttacatttct ttttcgaaat tcaaaaaagt atgaataagc tagcaaaaag
    31081 agctctgaaa tagagctctg tgaatgaagg taagaaattt ttcattcgat tttttcaatt
    31141 attagaaatt aagctttcaa tgtagatgca tatttgtttg caatctctat ccaccgacta
    31201 aaaaggtccc caggactata ttgatgatta cttcgtaatc tccatctgcc ggctaaaaca
    31261 atcctctgga tttgatgatt gcttcgcaat ctttatctgc aacctaaact agtctcccag
    31321 actataatga tgattacttc gtaatcttca tctgccgact aaaacaatcc actggattgt
    31381 tttagctacc tagatatgct tttctgactt cttctgatga ggcgagttct tttcctgttc
    31441 ctgataggac gattttccct gtttccagta catatcctcg gtcagagatt gcaagtgctt
    31501 tattggcatt ttgttcaatc aagaggacgg ttgttccttg cttctgaata tcttgaatga
    31561 tatcaaaaat ctcttggatg aagattgggg caagtcccat tgatggttca tctaaaagaa
    31621 gaagttttgg tgttgacata agagcgcgtc ccatggcaag catttgttgt tcccctcctg
    31681 aaagagtagc tgcatcttgg ttcttacgtt cttcaagacg aggaaagcgt gagaaaacct
    31741 tcttcaagtt agcttgattt tcttcacgat ttttctttaa gaaagctccc atttcaagat
    31801 tttccataac agtcaagcca ggaaagacgt ggcgtccttc tggaacttgt gaaagaccac
    31861 ctgccacaat tttctgagct ggcatttttt ggatttcttg acctaaaaat tcaatctttc
    31921 ctgaacttgg tcgaaccaaa cctgacaagg tgcgaagaat agttgtctta cctgcaccgt
    31981 tggcaccgat aagggaaaca acttctcctt cattaacttc aaagcttaca tcacggactg
    32041 cttggatcat accgtaatgc acagaaagat tttcaacttt taacatagac attaggcttc
    32101 acctcctaga taagcttcga taacgcgttt attggtctta atttcgtctg gagttccttg
    32161 agcaattaaa cggccatatt caagtacgta gatacgttct gttacttcca tgaccagatt
    32221 catatcgtgt tcaatcaaca tgattgtaat cttaaattca tctttgatac gacgaattaa
    32281 ctcagtcaat tcggctgttt cctgtgggtt catacctgct gctggttcat ctaagaagag
    32341 aattttaggt tccgtagcaa gggcacgaac aatttccaaa cgacgttgtt gtccgtagga
    32401 aagattttta gcaagagtct ctgcatcacc atctaaatca aagattttca acaattccaa
    32461 agctttagcc tttaattctt tttcactctt gtaaaaagct ggtaagcgta agaaactagt
    32521 aaaaacatgt tgtttgtgat ggtttccaaa agcaatcaaa acattgtcta aaactgttaa
    32581 atctttaaag agacggatat tttggaaagt acgtccaagt cccaaagagg caatcttata
    32641 aggtgatttc ccattcaaaa ggtgaccatc tagggttact gttccctcgc ttggttcata
    32701 aacaccggtc aaaaggttga aaagggtggt tttcccagct ccgtttggac cgattaatcc
    32761 aaccagttcc ccttcgttca attcaagagt cacatctcca acagctgtta gaccaccaaa
    32821 atgtttggtt aactgtttta cttcaagtaa tgccattagt tttgttcctc cttcttagat
    32881 tttttaaaga aacgtgatag gctcagttcc cacgttccaa ggagtccacc tggtctgaaa
    32941 atcattacca ataccaaggc caaagcgtaa ataatcatac gcacactagc aacatcttgg
    33001 agaagcatat tcaaaattcc cagaacaata gccgaaacaa tcgcacctgt aatggaaccg
    33061 agtccaccaa atacaacaat aatcaaaacg ttgattgagt tgatgaaggt gtaatctttc
    33121 ggtacaacag acccgataaa tcctgcctga agtgacccag caatacttgc agtaatggca
    33181 ccaaagacaa aagcgatgat tttaatttta gtcgtattaa ccccaactga ctcagcagcg
    33241 atttcatctt cacgaacaga gagggttgaa cggccaattg ggctacgcaa gaagttcaag
    33301 gttgcaatgg ttgtaatcac gacaaagaag taaaccattt gccaagttgt aaagttagga
    33361 atccctaaga tacctgccgc accatttgta agacttccac cattgatgat aaagatacgg
    33421 ataatttcag aaacacccag agttgctacc gcaagatagt cccccttcaa gcgcaaggtt
    33481 ggaatgccga caagtaaggc aactgctcct gaaagcaaag cccctacaag catagctcca
    33541 aagaaggcac cgtaggttgg tgatttagaa ccaataatag ctgctgcata ggcaccaatc
    33601 gccatgaaac cagcatgacc aagtgaaaat tgtcctgaaa aaccaacgat taagttgaga
    33661 ccaacagcca aaataatatt aattccaatt tgttgtaaaa tctgtacata gaatagatta
    33721 agtactccga ctgaaaccag tacactaatc aagctatagc cagctaacaa aaggagtaac
    33781 catagaatat taacttttaa attttccttc atcgtttaca ccttctcttt cacattctta
    33841 ccaaggatac cagctgggcg gacaatcaag atcaacaaca agattccata aacaatggca
    33901 tcacggaaat ctgacatccc aaaggctgtc gcaaaggttt ccaatagacc aatcacaaag
    33961 ccaccaagag cagcaccagg aataattccg ataccaccaa gtactgcggc aacgaaagat
    34021 ttaagacctg gagtaacccc catcaaaggc tcaagagagt tataataaag agcaatcaga
    34081 acaccagccg cacccgcaag agcagaaccc aaagcgaagg taaagctaat cgtacggttt
    34141 acattgatcc ccatcaattg cgccgcgtcg ctatctactg atactgcacg catggctttc
    34201 cccatcttag tcttttggac aatgacttgt aacaaaatca tcaaaatcaa ggaaatggcc
    34261 aaaatcatta actgcacatt tgttaagcta attggtccca aatcatatcg aactgtttga
    34321 atcgcttgag ggaaggcacg ggtattggca ccaaccagat agaccattcc atactccaat
    34381 aggaaagaaa ccccaatagc cgtaatcaaa acagcaatac gagtagagtg gcgcaaaggt
    34441 cggtaagcaa gaaactcaat cacgacacca agaatagctg tcgctagcat agctacaata
    34501 agcgctacaa agaaattcat ttggaaagaa ttgatcaaga aataaccgat aaaggctccc
    34561 atcatataaa tatcaccatg ggcgaagttg atgagcttga taattccgta aaccatggta
    34621 tatcctaggg ctaacagcgc gtaaacacta cctagaatca aaccatttac gagttgttgg
    34681 agcataagat tcactctttc tatttataat tccgaggggt ttccctcact ttttgatagg
    34741 ttcttatact caatgaaaat caaagagcaa actaggaaac tagccgcagg ttgctcaaag
    34801 cactgctttg aggttgtaga taagactgac gaagtcagtc acatatataa tccaaggcga
    34861 cgttgacgca gtttgaagag attttcgaag agtattaaat atcgaaacag ggagtgagtc
    34921 aaaggctcat tccctatttc aacatttttc tattatggtt ttacaacttc tgctgcttca
    34981 actttaccat tgttcatggt catcatgtaa gcagttttga ctgtgttgtg gtctgcatcg
    35041 aagcttgttt gaccagttac accttcaaaa tcttttgttt tagcaaggtt atccttgatt
    35101 tcacctgaat ttttagcacc ttttgctgcg tttgctacaa ggtgaactga atcataagcc
    35161 aaggctgcaa atgttgaagg ctcttcattg tacttagcac ggtaagcgtc aaggaaggct
    35221 ttagctttag ctgaaacttc tacagtagtt gagaagcctg agataaagta gatgtttgat
    35281 gctttttcag cagttgcttg ttgtacaaac tcctcaccgt tgaatccatc accaccaacg
    35341 attggtttgt caattcccat gccacgcgct tggtttacaa tcttaccagc ctcattatag
    35401 taaccaggaa caacgatagc atcaaagtct ttccctttca tttttgtaag ggctgcttga
    35461 aagtctgtgt cacctgctac gaaagtttca tctgcaacga tttcaccctt gtatgactcg
    35521 cggaaagatt tggcaatccc tttagcatag tcactggcat tgtcagtgta aagaacaact
    35581 ttcttagcat ttaatttttc agaaacatag tttgagataa tttttccttg gaagctatct
    35641 tggaaagttc caataaagag gtaatcttga cctttagtca atccatcttg agtcgcactt
    35701 ggtgagatca atggaacacc tgcttttgta gcgttcgcta ccgcagctgc agtcgcacca
    35761 gatgtcgcag gtcctacgac tgctgatact ttagattggg ttacaaggtt agttgtaact
    35821 gaagcagcct cagctgtttc agacttatta tctttatcga ctacttcgat ttgttttcca
    35881 tcgataccac ctgcggcatt gatttcatca acagccaatt gggcaccttt ttgttcagct
    35941 gttccgtatg cagctaaaga acctgattct tcaaagttaa acccgatttt aattgtcttt
    36001 tcctctactg agttaccagc agtgttgact gctccagact tcacttctcc acaggctgca
    36061 agaagtgcta cacttgcaag cgccacaaac gatagggcaa attttttctt catctttttg
    36121 tctccttatt tcttaaacag tctgttaaaa atataccgaa ttatcagaaa attgtcaatg
    36181 ttttccttga actttttcga tgataacgtt ttcctatcga taaagattgc ccacaaaggg
    36241 tgtttccaac tcttggatat gacaaaccct aactttttta ataaattttt ccttgctcaa
    36301 gcgtccgacc aattgctcca cttcttgagt tggaacatag agttgtaagt aacgatgttt
    36361 cttggaatga taggtaatat ctccataatc ctgcagtttt ttggcatcac gattatagta
    36421 aagatagata atcaagccag agcgattgac tttttcaaac atgataaatc ctctttctaa
    36481 gaaatctctc cctattatac caaaaaaagc aaacccagtc gagtttgcct tctatcatca
    36541 ttagttcaat gaattgttgg ccataatttc atcaataaag ccatattcaa gtgtttcctg
    36601 ggcgctcatc cagttatcac gttctgcatc tgcatggact ttttccattg actgacctga
    36661 attttcagcc aagatttttt ccaaggtatt acgagttttg agcaagtgtt ctgcagcgat
    36721 agccatatca gtttgttggg taccaccacc tgtaccgccc attggttggt gaatcatgta
    36781 ttcagcattt ggaagcatga aacgtttgcc ttttgctcca cttgatgcga tgacagtccc
    36841 catagatgca gccattccca taacaatggt ttggacatct gccttgataa agttcatggt
    36901 atctacgatt gccaaaccag ctgaaacaga accacctggt gtattgacat aaaggtaaat
    36961 atctttcgta ctatcttggg catccaagaa aagcaattgg gcaataacag agttagccat
    37021 attgtcttca accggacctg tcagcataat gatgcggtct ttgagaagac gtgagtaaat
    37081 atcgtaagaa cgttctccac ggcttgtttg ttcaataact acaggaatca ttcatttctc
    37141 cttttgagtt ttaattttgt tggtcaaatg actgaagata agactattat aatatcttgg
    37201 tcaaaaaagg tcaaattttt gctctgcttt cattagacag aaacaaaaac ccaacctcct
    37261 ttcgtgacta gaaatacttt tccaagtcat tcttcttttc gatcttattt tgtaccgaac
    37321 aagcggtctc cagcatctcc aagacctgga acgatataac cgtgttcatt caaacgttca
    37381 tccaaggctg ctgtaaagat ttctacatct ggatgagctt cttgaagggc ttttacaccc
    37441 tctggagcag atacaaggca gacaaatttg atatttgatg cgccacgttt tttaagagaa
    37501 tcaacagcca agattgctga gccacctgtt gccaacattg ggtctactac aaaaatttga
    37561 cgttggtcaa tgtcctcagg caatttcacc aagtattcaa ctggttgaag tgtttcttca
    37621 tcacggtaca taccgatgtg gccaacttta gcagctggaa ccaagctcaa gagaccatca
    37681 accatcccga tacctgcacg caagattggg acgatggcca atttcttacc tgccaattgt
    37741 ttttgaactg tttttgtaat tggtgtttcg atttccacat cttctagtgg aagatcacga
    37801 agtacttcat accccatcaa cattgcaatc tcatctacta gctcacgaaa agcttttgta
    37861 gaagtatctg tacgacgcaa gattgacaat ttgtgttgaa tcagtgggtg attaataact
    37921 tcaatttttc ccatttttgg aattccttct ttcaatttat tcttcttatt ataccaaaaa
    37981 acggtttaaa aatctttcta aaccatttat ttttgataat ttttacatta gatcagcctc
    38041 tttaagagct gtctgtactg tctcaagtgg taaatgggtc aattctgtcc ctttttcttg
    38101 ataaaggtat tgggcgtagt cgtccattcg gtactggttg atataaacca cacgcttgca
    38161 gccgacctga agcaattgtt ttgtacagtt gagacaagga aaatgggtta cataggctgt
    38221 aaagcctttg ggaacaccac gctcagcacc ttgaaggata gcattgacct cagcgtgaag
    38281 ggtgcgaaca cagtggcctt caatgaccaa acattcgtga tcaatacaat gctcagtccc
    38341 tgacaccgaa ccattgtaac cagtggaaat aaccttatta tcttttacca gaatcgcgcc
    38401 cactttagca cgtttacaag tggaacgatt cgcaattagt agggcttggg ctgcaaaata
    38461 ctcatcccag gccagtcttt tttcagtcat ctcttttctc ctttttctct attttttaaa
    38521 aaatggtaaa cctaaatctg caatcttttc agctggtacc ttcatgccat ccttgatcca
    38581 ttttagaagg acagagacaa tggctgagct ccagaaggaa tgaagataag agctgacacc
    38641 ttttgatttc ccatggtatt tttctagaaa ttcctgcatg gcttggacaa agattttttc
    38701 cagatggtaa tccaaggcca attgaatcac tctagcttcc tttctggcct cccggaaaag
    38761 gtgaacccaa accaaataaa ggtctgtctt taaatcgtaa tgatgcagct gttccataat
    38821 attgtggaca gttcgtttaa agacgctctc taaaatttcc tctttggagt cataattgcg
    38881 ataaaaggcc gcacgcgaaa cacctgcacg tttgaccaat tcagaaatac taatcttggt
    38941 cagctccttt ttttccaaga gttgcaagag ggctgtttca atggcttctc tggttaataa
    39001 attggattct tggtttgatt ttctgagatt ttcaagagac ttttcagaga ttctacgttc
    39061 agacataaca ttttctttct acttgtcaca acagacggat gatgcttttg tttcaagagt
    39121 tttcatgata taattgtaaa aaaagacaga acctttgtca atgtataact gacaaagatg
    39181 gagaatttct atgacttgga aaattattgc tgactctggt tgtgattatc gtcaactgcc
    39241 aacaccagct attaacacaa cctttgtaag tgtcccctta accattcaag tagctgatca
    39301 ggtctttgtt gatgacgcca gtctcgatat tgaccaaatg atggaaacca tgtatgcaac
    39361 tgcagaagct tcaaaatcgg cttgtccaag cccagatgac tatttgcgag catttgaagg
    39421 tgccaaaaac attttcctag taaccatcac aggtaccctt tctggcagtc acaatagtgc
    39481 tcaactagca aagaatattt atctggaaga ccatcctgac actaagattc atgtgattga
    39541 tagtttatct gctggtggtg aagttgacct actcgtagaa aaattgaatg acttgattga
    39601 ccagggcttg tcttttgaag aagtggttga agctatcacc gcctatcaag aaaaaactaa
    39661 gttgctcttt gtcctagcca aagtcgataa cttggtgaag aacggccgtt tgagcaagct
    39721 tatcggtacg gtcgttggcc ttctcaacat tcgtatggtc ggaaaagcta gtgaaactgg
    39781 aactctcgaa ttgctacaaa aagcaagggg atcaaagaaa tcagttcaag ctgcctatga
    39841 tgagttagta aaagctggat atgctggtgg ccgtattgtc atggcccaac gcaataacga
    39901 aaaatgttgt caacagctct cagagcgaat ccgtgaaacc ttcccacaag cggatattaa
    39961 aattctacca acctctggtc tctgcagttt ctatgcagaa gagggcggtt tgctgatggg
    40021 ctatgaaatt gattaattgc attcttgaca agaggtgacc atcatctctt gtttttttgt
    40081 ggtacaatag agctatgaaa cattttgata ctattgtcat cggtggggga cctgctggta
    40141 tgatggctac gatttccagt agcttttatg gacagaaaac cctcctcatc gaaaaaaatc
    40201 ggaaacttgg aaaaaaatta gctgggactg gtgggggacg ttgcaatgtg accaacaatg
    40261 gtagcttaga caacctgcta gctggaattc ctggaaacgg acgctttctt tacagtgttt
    40321 tctcccagtt cgataatcat gacatcatca acttttttac agaaaatggt gttaaactta
    40381 aggtcgaaga ccacggacgc gtctttccag ccagtgacaa gtctcggact attatcgaag
    40441 ctttggaaaa gaaaatcact gaactaggtg gtcaagttgc tactcaaata gaaatcgttt
    40501 ctgttaaaaa agtagatgac cagtttgtcc ttaagtcagc ggatcaaacc ttcacttgtg
    40561 agaaactcat tgtcacaaca ggtggtaagt cttatccttc gactggttcg actggttttg
    40621 gtcacgagat tgctcgccat tttaagcata ccatcaccga tcttgaggct gctgaaagtc
    40681 ctttattaac agattttcca cataaagcct tacaagggat ttctctggac gatgtgaccc
    40741 taagttatgg taagcatgtc atcactcatg atttactctt tacccacttt ggtttgtcag
    40801 gtcctgctgc cctacgcatg tctagctttg tcaaaggtgg ggaggttctc tcactcgatg
    40861 ttttgcctca actttctgag aaggacttgg ttacatttct agaagaaaat cgggaaaaat
    40921 ccttgaaaaa cgctttaaaa accttgttac cagaacgctt ggccgaattt tttgtacaag
    40981 gatatcctga aaaagtcaaa cagctgactg aaaaggaacg agaacagctt gtccagtcca
    41041 ttaaagaact taaaattcct gtaactggaa aaatgtccct tgcaaagtcc tttgttacca
    41101 agggtggagt cagtctcaag gaaatcaatc ctaaaaccct tgaaagtaag ctggtacctg
    41161 gcctccactt tgcaggcgaa gttatggata tcaatgccca cacgggtggc tttaacatca
    41221 cttctgccct ctgtaccggc tgggtggcgg gaagtctgca ttatgattaa agattaaagg
    41281 ttgtgaagaa tgaacagaag agaggcagtc gaatttgtta acatgtgtat gattaaaaac
    41341 ggagataagg tcctggtcca agaccgagtt agtcccgact ggcctggcat tacttttcct
    41401 ggtggtcatg ttgaacgtgg cgaatccttt gtcgatgctg tcattcgtga agtgaaagaa
    41461 gaaactggtc tgatcatttc caaaccccaa ctctgtggta tcaaaaactg gtatgacgac
    41521 aaggattatc gttatgtcgt ccttttttac aagacagaac actttactgg tgaactccag
    41581 tcttcagacg aagggaaagt ttggtgggag gactttgaaa atctttctca tctaaaactt
    41641 gcaaccgatg atatgtctga tatgcttcgt gtgtttctag aagaagatct cagtgaattc
    41701 ttttactaca aaaacggtga cgactggctt tatgatttga agtaaaaaat aggctaggaa
    41761 attcctagcc tattttttct gcatttgtct gaacgtaagc tgcaatggca tctgacgtgt
    41821 actgggctgt tccagaacca aacttgtcaa tgttttcctt aaactctggg ttatagacgt
    41881 aacctttacc gatatgaccg aatacctcaa tagagcagtc aaatccataa gtgcgaatgg
    41941 cttgcaagag cttggctgct tgctcttggt tttcggttgc tgttgcaggt aaaccaactt
    42001 gaagattttg tgccaaagtt tgaaagactt ggttaaaggc ggccgtagcc tcgtcttcgt
    42061 gacctttttg gcgttcgagc gcttgtccca tgacttcttg accatatttc tctaccgctt
    42121 cttggtggta tttttgattg tcttgatagc taaatcccgt gaatttttcc tcaatggtca
    42181 tttttctttc tcctttttgt tcttgaatag ttttttgcaa ggtggaaatc aaggtatcca
    42241 gatgttgcct ttcgcgagtt agatagtcca actgcctagt caaatggggc aataaatctg
    42301 tcctttcttc ctttaacagc tctgctattt tctctaaaga aaagcctaga tatttgtagt
    42361 aaagaatgac ctgaaggcgt tccaaatcct cttgactgta ggttcgatag ccgttttccg
    42421 actttaaggg gaccaagagt cctatcttgt catagtgatg cagggtcttg acagagatac
    42481 ctgaaagctg cgcagcttct tttatatggt acatgatttc ctccttacaa tgatagtata
    42541 acccctcccc ttaggtcaga gtcaagcgtt tttgagaaaa ttttttaact tttttgagaa
    42601 aggtgtgaaa acttgaaaat gattttcttg acagacctta gaaaacatga taggatagtc
    42661 aagtctatca atcaaacaga aggctatttg acaactcaag tagctttttc ttattttgaa
    42721 aaaggagatc agagtttaac tatgtcagaa aaatcacaat gggggtcgaa acttggtttt
    42781 attctagcat ctgctggctg gccatcgggc ttggtgccgt ttggaagttt ccctacatga
    42841 ctgctgctaa tggcggtgga ggctttttac taatctttct catttccact attttaatcg
    42901 atttccctct cctgctggct gagtttgccc ttggccgtag tgctggcgtt tccgctatca
    42961 aaacctttgg aaaactgggc aagaataaca agtacaactt tatcggttgg attggcgcct
    43021 ttgccctctt tatcctctta tctttttaca gtgttatcgg aggatggatt ctagtctatc
    43081 taggtattga gtttgggaaa ttgttccaac ttggtggaac gggtgattat gctcagttat
    43141 ttacttcaat catttcaaat ccagccattg ccctaggagc tcaagcggcc tttatcctat
    43201 tgaatatctt cattgtatca cgtggggttc aaaaagggat tgaaagagct tcgaaagtca
    43261 tgatgcccct gctctttatc atctttgttg ttatcatcgg tcactctctc agcttgtcaa
    43321 atgccatgga aggggttctt tacttcctca aaccagactt ttcaaaactg actagcgctg
    43381 atctcctcta tgctctggga caatctttct ttgccctctc actaggggtt acagtcatgt
    43441 tgacctatgc ttcttacttg gacaagaaaa ccaatctagt ccagtcagga atctccatcg
    43501 tagccatgaa tatctcgata tccatcatgg caggtctagc cattttccca gccatgtcag
    43561 ccttcaatat ccagtctgaa gggggaccga gcttgctctt tatcgtcttg cctcaacttt
    43621 ttgacaagat gccttttgga accattttct acgtcctttt cctctttgcg actgtcactt
    43681 cttctgtcgt gatgctggag atcaatgtgg gcaatatcac caaccaggat aacagcaaac
    43741 gtgccaaatg gagtgttatt ttaggaattt tgacctttgt ctttggcatt ccttcagccc
    43801 tatcttacgg tgtcatggcg gatgttcaca tttttggtaa gatcttcttt gacgctatgg
    43861 acttcttggc ttccaatctc ctcatgccat ttggagctct ctacctttca ctttttacag
    43921 gctatatctt taaaaaggct cttgcaatgg aggaactcca tctcgatgaa agagcatgga
    43981 aacaaggact gttccaagtc tggctcttcc ttcttcgttt cttcgtttcg tcattccaat
    44041 catcatcatt gtggtcttca ttgcccaatt tatgtaatca aaaaggactt gagtagtgaa
    44101 ctcaggccct ttctttttat ggatggctaa caatcaattc caaatcttgc ccttctagag
    44161 tccaagattc aacatcactt ggtaggataa agtgtctccc tttttggatt ggatagtttt
    44221 tcccgtcaac agttagttga ccttgaccag ccaagacact caacaagctg tagtcagctg
    44281 tcttttcaaa gttaactttt ccagtaattt cccatttgta aactgcgaag aaatcattgg
    44341 atacaaggag agtggaacgt agatcatctg ctttgattgt cactggacgg ctatttgcgg
    44401 gttcaccaat gttcaagaca tcgatagatt tttcaaggtg aagttcacgt aaattgccct
    44461 tgtcatcctt acggtcaaag tcatagacac ggtaagtggt atcgctagac tgctgggttt
    44521 caagaatcaa gatacccgcc ccgatagcgt gcatggtacc gcttggtaca tagaagaaat
    44581 ctcctgcttt aactggcact tttgtcaaca gcgcatccca gttcttatcc tcgatttgct
    44641 ggcggagttc ttcttttgac ttggcattgt gaccgtagat aatctctgaa ccttcatctg
    44701 ctgcaatgat gtaccaacat tctgtttttc caagttcgcc ttcatgctcg agtccataag
    44761 catcgtctgg gtgaacttgg acactgagcc agtcgttggc atcgaggatc ttggtcaaaa
    44821 gtggaaatac aggctcagga cgattgccaa ataattcacg gtgttccgca tacaaagtag
    44881 caagatctgt tccctcgtaa cgaccattgg caactttaga aacaccattt agatgagctg
    44941 agatagccca gtactctccg actttttcac ttgggatgtc gtagccaaac tcatcacgta
    45001 gcttggtacc accccagatt ttttcttgca taactgattg taaaaataat ggttctgaca
    45061 tcttaatctc ctgtctgatt tttctcccct cattatagca aaaaaagagt tccatttgaa
    45121 ctctttttta catcttataa agcagggaga agattttata aaaatagtaa acaaatgtgc
    45181 tctactcgat gcttacacca ttgctatcaa tgacatcctt gtaccaatag aaggacttct
    45241 tcttgctacg tttgagagtc ccatttccta cattatctcg atctacatag ataaagccat
    45301 agcgcttatt catttcccct gtgccagctg aaaccggatc gatacagctc caagtcgtat
    45361 aaccaagcaa gtcaaccccg tcttggtaaa tggcatctcg catggccttg atgtgggcct
    45421 ctaagtaagt aatccgatag tcatctgcta cataaccatt ctcatccggt gtatccatag
    45481 caccgagtcc attttctacg ataatactaa actaaaatca aaaagcatta tacaata
//
LOCUS       DAVLYV010000009        41693 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLYV010000009 DAVLYV010000000
VERSION     DAVLYV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.009"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            69..558
                     /locus_tag="VYO70_000492"
                     /pseudo
     CDS             69..558
                     /locus_tag="VYO70_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536889.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            574..1053
                     /locus_tag="VYO70_000493"
     CDS             574..1053
                     /locus_tag="VYO70_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712775.1"
                     /translation="MKLNKQKNRMIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIP
                     GDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMM
                     MNTSNDLFIKAIELLPFYSLPVFVASNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
     gene            1439..1648
                     /locus_tag="VYO70_000494"
     CDS             1439..1648
                     /locus_tag="VYO70_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1712776.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            1638..2126
                     /locus_tag="VYO70_000495"
     CDS             1638..2126
                     /locus_tag="VYO70_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712777.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYIIYLPWIFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            2242..3702
                     /locus_tag="VYO70_000496"
     CDS             2242..3702
                     /locus_tag="VYO70_000496"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1712778.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            3699..4523
                     /gene="nadE"
                     /locus_tag="VYO70_000497"
     CDS             3699..4523
                     /gene="nadE"
                     /locus_tag="VYO70_000497"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1712779.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            4873..5424
                     /locus_tag="VYO70_000498"
     CDS             4873..5424
                     /locus_tag="VYO70_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1712780.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            6155..6847
                     /locus_tag="VYO70_000499"
     CDS             6155..6847
                     /locus_tag="VYO70_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1712781.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGYVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYNV
                     DGKSGQVTESTLSQKKDSQTRVVKKRTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
     gene            6901..7290
                     /locus_tag="VYO70_000500"
     CDS             6901..7290
                     /locus_tag="VYO70_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1712782.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNSSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(7951..8979)
                     /gene="queA"
                     /locus_tag="VYO70_000501"
     CDS             complement(7951..8979)
                     /gene="queA"
                     /locus_tag="VYO70_000501"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1712783.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKKNGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            9130..9837
                     /locus_tag="VYO70_000502"
     CDS             9130..9837
                     /locus_tag="VYO70_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1712784.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            9977..10153
                     /locus_tag="VYO70_000503"
     CDS             9977..10153
                     /locus_tag="VYO70_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1712785.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            10283..11218
                     /locus_tag="VYO70_000504"
     CDS             10283..11218
                     /locus_tag="VYO70_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1712786.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPKLLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLFGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            11211..11999
                     /locus_tag="VYO70_000505"
     CDS             11211..11999
                     /locus_tag="VYO70_000505"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1712787.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            12000..12383
                     /locus_tag="VYO70_000506"
     CDS             12000..12383
                     /locus_tag="VYO70_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1712788.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            12399..12788
                     /locus_tag="VYO70_000507"
     CDS             12399..12788
                     /locus_tag="VYO70_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1712789.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYALQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            12872..14002
                     /locus_tag="VYO70_000508"
     CDS             12872..14002
                     /locus_tag="VYO70_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1712790.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            14007..14744
                     /locus_tag="VYO70_000509"
     CDS             14007..14744
                     /locus_tag="VYO70_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1712791.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIAEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWKEIKKA"
     gene            14755..15528
                     /locus_tag="VYO70_000510"
     CDS             14755..15528
                     /locus_tag="VYO70_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1712792.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKENPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            15518..16135
                     /locus_tag="VYO70_000511"
     CDS             15518..16135
                     /locus_tag="VYO70_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1712793.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLTLVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            16345..16746
                     /locus_tag="VYO70_000512"
     CDS             16345..16746
                     /locus_tag="VYO70_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1712794.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            16746..17024
                     /locus_tag="VYO70_000513"
     CDS             16746..17024
                     /locus_tag="VYO70_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1712795.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            17014..17787
                     /locus_tag="VYO70_000514"
     CDS             17014..17787
                     /locus_tag="VYO70_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1712796.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            17788..19092
                     /locus_tag="VYO70_000515"
     CDS             17788..19092
                     /locus_tag="VYO70_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1712797.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            19666..20544
                     /gene="pstS"
                     /locus_tag="VYO70_000516"
     CDS             19666..20544
                     /gene="pstS"
                     /locus_tag="VYO70_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1712798.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            20544..21461
                     /gene="pstC"
                     /locus_tag="VYO70_000517"
     CDS             20544..21461
                     /gene="pstC"
                     /locus_tag="VYO70_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1712799.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            21451..22335
                     /gene="pstA"
                     /locus_tag="VYO70_000518"
     CDS             21451..22335
                     /gene="pstA"
                     /locus_tag="VYO70_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1712800.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            22346..23149
                     /gene="pstB"
                     /locus_tag="VYO70_000519"
     CDS             22346..23149
                     /gene="pstB"
                     /locus_tag="VYO70_000519"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1712801.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            23162..23920
                     /gene="pstB"
                     /locus_tag="VYO70_000520"
     CDS             23162..23920
                     /gene="pstB"
                     /locus_tag="VYO70_000520"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1712802.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            23932..24585
                     /gene="phoU"
                     /locus_tag="VYO70_000521"
     CDS             23932..24585
                     /gene="phoU"
                     /locus_tag="VYO70_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1712803.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            24742..25557
                     /locus_tag="VYO70_000522"
     CDS             24742..25557
                     /locus_tag="VYO70_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1712804.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(25577..26848)
                     /locus_tag="VYO70_000523"
     CDS             complement(25577..26848)
                     /locus_tag="VYO70_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1712805.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFDQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRAILDLAAKYSVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNTIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            26990..27721
                     /gene="budA"
                     /locus_tag="VYO70_000524"
     CDS             26990..27721
                     /gene="budA"
                     /locus_tag="VYO70_000524"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1712806.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKESGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFSHMHVRMIPKSTPDTKFADIATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            27731..28483
                     /locus_tag="VYO70_000525"
     CDS             27731..28483
                     /locus_tag="VYO70_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1712807.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            28595..29500
                     /gene="murB"
                     /locus_tag="VYO70_000526"
     CDS             28595..29500
                     /gene="murB"
                     /locus_tag="VYO70_000526"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1712808.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARVVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            29660..30817
                     /locus_tag="VYO70_000527"
     CDS             29660..30817
                     /locus_tag="VYO70_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712809.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            30798..31604
                     /locus_tag="VYO70_000528"
     CDS             30798..31604
                     /locus_tag="VYO70_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712810.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WLNLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            31601..32374
                     /locus_tag="VYO70_000529"
     CDS             31601..32374
                     /locus_tag="VYO70_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1712811.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            32371..33441
                     /locus_tag="VYO70_000530"
     CDS             32371..33441
                     /locus_tag="VYO70_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1712812.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKLEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDPQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENAFQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            33686..33925
                     /locus_tag="VYO70_000531"
     CDS             33686..33925
                     /locus_tag="VYO70_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712813.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTVILSHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            34200..34685
                     /locus_tag="VYO70_000532"
     CDS             34200..34685
                     /locus_tag="VYO70_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1712814.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSEISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            34707..37325
                     /gene="alaS"
                     /locus_tag="VYO70_000533"
     CDS             34707..37325
                     /gene="alaS"
                     /locus_tag="VYO70_000533"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1712815.1"
                     /translation="MKQLSSAQVRQMWLDFWATKAHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDSSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(37415..38869)
                     /locus_tag="VYO70_000534"
     CDS             complement(37415..38869)
                     /locus_tag="VYO70_000534"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1712816.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPIADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            39048..39797
                     /locus_tag="VYO70_000535"
     CDS             39048..39797
                     /locus_tag="VYO70_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712817.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQDLSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSSSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDLDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGKVED
                     LRRDNPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            39778..41412
                     /locus_tag="VYO70_000536"
     CDS             39778..41412
                     /locus_tag="VYO70_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712818.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLIFFSTIAIHFPFEEMPVYFSFVVAILLVIAFSTSLTAFYNVFYESK
                     DLASYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLALYISLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTTAFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFLPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPILTLLGLSLYLGLPIISMIYLIMAWILASVIL
                     SCHHYFKDVKNLSINWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
BASE COUNT        12012 a         7770 c         9408 g        12503 t
ORIGIN      
        1 gatttagaag tttgatccaa gagttagatt tgactcagtc tattaataaa aaagtaaaga
       61 cctattcttt gggtacaaaa caaaaattag ctttgcttct aactctcgtt acggaacctg
      121 atatattgat tttagatgaa ccgactaatg gtttagatat tgaatcatca caaatagttt
      181 tagcggttct aaaaaaaatt agctttaaat gaaaacgtgg gaattttaat atcgagtcat
      241 aaattagaag acattgaaga aatttgtgag agggttcttt tcttggagag cgggcttttg
      301 acatttcaaa aagtaggaaa agatagtcat aatttcttgt ttgagatagc tttttcatca
      361 gctacagata gagacatttt cattaccaaa caagaatttg gggatattgt tcaggaagag
      421 ggattgagaa ttactatgtc tgggaatatt caaaatagtg agctttttaa attttttaac
      481 gaaaactcta ttaaagtagt tgattttgaa actaaaaaag agacgcttaa agatatttac
      541 ctaaatcgtt caaaataaag gaaggttata atcatgaaat taaataaaca gaagaatcgg
      601 atgatttacg tcttgtctaa ttttctatat gccatctcag tttccattat ttatgctttg
      661 aatggcattg tgttactagt catagtaagt aaattgggta ttccaggtga tttaggatta
      721 gattttatag tagctattgt agtcaataca attttgttag tcctgtttta ttttctatta
      781 tcttacattt tttatttata caaattgaaa agtggcttgg tatttggtat tttagtagct
      841 ttactactct ttatccctaa tatattaaat acgatgatga tgaatactag taatgatttg
      901 tttatcaaag caattgaact tcttcctttt tattctttac ctgtatttgt ggcttcaaat
      961 acgatgtcta ttagtcagta tcttgtggta atcactacaa tcattttatt gtactttttc
     1021 actctcaaga aaagcaagaa gtattcattt tagtttcatt tagtattatt ttgcatacct
     1081 aaaatacaat aaaaaaatca gtcatcttgg tatgctcctg ctttcactat tcaacacgtt
     1141 tttgacttat actaggctca tttccaaaag cattatataa tagtgatatg aaaccaacta
     1201 aactaaacaa gaaatataag caataaaaac tcgtttaaaa gatcctacta aagctaatac
     1261 taaataaaaa taaaagagta aactaggaag tttatttcaa acaacctaaa atactgattt
     1321 tcggctgaag ataatactgg agtgcaaatt aatggggtta taataaatag ctgatagctt
     1381 gtgttggttt tggatttttt aagagtagat gagtattaaa actataagga ggacgaaggt
     1441 ggctaaaaat ttaaaattaa aattagctcg ggtagagcgt gatttaacac aaggtcaact
     1501 ggcagaggct gtcggggtga cgcgccagac tattggtttg atagaagcgg ggaaatacaa
     1561 tcccagtctc tcgctctgcc agtccatttg cagatgttta gggaaaaccc tagaccaact
     1621 attttgggag gaagaagatg aaaaatagat tttattatta tcaattacta gacgaaagag
     1681 aagaacaact gttcaataaa gcgggctctg aaagtttcta tatctgcatc gctttgtcgc
     1741 tcctatctta tatcatttca gtattagcac caagcctttt taattctaat atgctgctaa
     1801 tcgttatcat catagggaca ttttactttt tcaatcgtgc ccgttatctg ggagtgacct
     1861 actatagtcg ttttcatttt acgattttgg gttgtttttt cctaaccttg gctattacgg
     1921 ctcttttgat gttgcagaat tatcaattca acatagaaat ttatcagcac aatcctttga
     1981 attttaaata cctgtctgct tgggtcatta cttatatcat ttaccttccg tggatcttta
     2041 ttggcaatct tggtcttaag agctatggcg aatgggctca gaaaaaattt gaacaagata
     2101 tggatgaatt ggagagtgga gaatagcttg ttactctttt ctcaatccag ctaaaatgtg
     2161 atataatagt actaatttat tggaatacat gaaagttctt gaaaattttc atgggtttct
     2221 agctaaggaa gtaggaaaag tatgtatcca gatgatagtt tgacattgca cacggacttg
     2281 taccagatca acatgatgca ggtttacttt gaccaaggga ttcacaataa gaaggcggtc
     2341 tttgaggtgt atttccgcca acagcctttt aagaacggct atgcggtttt tgcaggtttg
     2401 gaaagaattg tgaactatct tgaagacttg cgtttttcag atagtgatat agcctatttg
     2461 gagtcgcttg gttatcatgg ggcgttcttg gattaccttc gcaatttcaa gttggagttg
     2521 accgttcgtt ctgcccaaga aggggatttg gtttttgcta atgaaccgat tgtgcaggtg
     2581 gaaggacctc tagcccaatg tcagttggtc gaaacggctc ttttgaacat cgtcaactac
     2641 cagaccttgg tggcgacgaa ggcagctcgt attcgttcgg ttatcgaaga tgaacccttg
     2701 atggagtttg ggacacgtcg ggctcaagaa atggatgcgg ccatctgggg aacacgcgca
     2761 gctgtgattg gtggcgccaa tggaactagc aacgtgcgtg cgggtaagct ctttgacatt
     2821 cctgttttgg gaacccatgc ccatgccttg gtacaggttt atggcaatga ctatgaagct
     2881 ttcaaggctt acgctgcgac ccacaaaaat tgtgtctttc ttgtggatac ctatgacacc
     2941 cttcgcatcg gtgtaccagc tgccattcag gtggcgcgtg agctgggtga tcagattaac
     3001 tttatgggtg tgcggattga ctctggggat attgcctaca tttctaagaa agtccgtcag
     3061 caactggacg aggctggatt tacagaggct aagatttatg cttctaatga tttggacgaa
     3121 aatactatcc tcaatctcaa gatgcaaaag gccaagattg atgtctgggg cgtgggtacc
     3181 aagctgatta cagcctatga ccagccagct cttggtgcag tttacaagat tgttgcaatc
     3241 gaagatgaaa ctggtcagat gcgcaatacg attaagctgt ctaataatgc tgaaaaagtt
     3301 tctacgccag gtaagaagca ggtgtggcgc attaccagtc gtgaaaaagg caagtcagaa
     3361 ggcgactata tcacttatga tggtgtggat attagcgaca tgacagaaat caagatgttc
     3421 catccgacct atacatacat caagaagacg gttcgtaatt ttgatgccgt tcctctcttg
     3481 gtggatatct tcaaagaagg aatattagtt tacaacttgc ctagtttgac tgacattcag
     3541 gattatgccc gtaaagaatt tgacaagttg tgggatgagt ataagcgtgt gctcaatccg
     3601 cagcactatc cagtggattt ggcgcgtgat gtatggcaag ataagatgga cttgattgat
     3661 aagatgcgca aggaagccct tggtgaagga gaagaagaat gagtttgcaa gaaacgatta
     3721 tccaagagct gggtgtcaaa ccagtgattg atgcccagga agaaatccgt cgttctattg
     3781 atttcttaaa aagatatctg aaaaaacatc ccttcctaaa aacctttgta ctagggattt
     3841 ctgggggaca agactcaacc ttggcagggc gtttggcgca attagctatg gaagaactgc
     3901 gagctgaaac gggagacgat agctacaaat ttatcgctgt ccgcctgcca tacggagtgc
     3961 aagctgatga agcagatgct caaaaagctc tagccttcat ccagccagat gtcagcttgg
     4021 ttgtgaatat caaggaatca gctgatgcca tgacagctgc agttgaagcg acaggtagtc
     4081 ctgtttcaga cttcaacaag gggaatatca aggcacgttg ccgtatgatt gctcagtatg
     4141 cccttgctgg ttcccatagc ggagcggtca ttggaacaga ccacgccgcg gaaaatatca
     4201 caggtttctt taccaagttt ggtgacggcg gtgcggatat tctccctctt taccgcctca
     4261 ataaacgcca aggaaaacag ctcttgcaga aacttggcgc agagccagcc ctttatgaaa
     4321 aaatcccaac ggcagaccta gaagaagata aaccaggcct agctgacgaa gtcgcacttg
     4381 gagtcaccta cgcagagatt gacgactacc tagaaggcaa aacaatcagc ccagaagctc
     4441 aagcgaccat tgaaaactgg tggcacaaag gccaacacaa acgccactta cccatcactg
     4501 tatttgatga cttttgggag taaaaaggtc cgggggacct ttttagcttc ttgccctgaa
     4561 attaaaaagc aagaaaaacc tccactggag gttttcagcc tctcatctta aaataagaaa
     4621 gtgagagaag gtctggggga tcttgaaccc cgagtttaga aataagaaaa tgaggcagat
     4681 tcggtaactc gaagagttcg atttcatcgt cttacccctg caacgatgac taggtttgaa
     4741 aaagcttgct agatcgcatt tcaaaccagg cagcgattgc gtcaagaaat tagaagacaa
     4801 actcgttttc tagctgttac tgagttgagc ctttttacta cgagcataga aataaggaag
     4861 tgaggtagca tcatgaaatc tattggtacg caaatattac agacagaacg tttgatttta
     4921 agaagatttg tggagagtga tgcagaagct atgtttcaaa attgggcttc atccgctgag
     4981 aatctgacct atgttacctg ggatccccat cctgatatcg aaatcactcg aaactcgatt
     5041 cgcaattggg ttgcttccta tactaatctc aactattata aatgggccat ttgtctaaaa
     5101 gaaaacccag agcaagtgat aggagatatc agcattgtta agatagacga ggctgattta
     5161 agctgtgaaa tcggctatgt gctaggcaag gcttactggg gacatggtat gatgacagag
     5221 gccttgaaag ctatcttgga cttttgtttt actcaagcag gttttcaaaa ggtcagagca
     5281 cgttatgcca gtctcaaccc agcttcaggt cgtgtcatgg aaaaggctgg aatgtcctat
     5341 ctacaaacca ttgttaatgg tgtagagaga aaaggctatc ttgcggatct tatttattat
     5401 ggtataagta gggaagaatg ttgaattcta ttttctgttt ctatcgaagt caactattta
     5461 ttgtaaatat aataattagc attccaagtt tatttgaaac tttaaaatag catattgatt
     5521 agtacaagac agatgttcta gttccttctt taatctggtt tagtattagt taaaaaatcg
     5581 ctttaagctt gtaactaaga gggacctaat cgactagatt ctccagccga acaggtggta
     5641 atgtactttt tatagtgtaa tcctagctgt tgttaaattt aaaatagaat cctctatcga
     5701 gttagggaat taaattcaac caattttatt catgtttttt ctatcaaatt atctaatatt
     5761 aaaatagtct cattctgatg agaaaactat tctatttata ctgcatagtt gtataaggaa
     5821 tgatgaaaga tgagtgaaag ttatcaagtt ggaatgtttg tatctaaata tattagcatg
     5881 tatttagata agatgtctgc aatcctttat atatgagatt gattacgata tagttaaagc
     5941 gttagttcaa taatcaatat atcattctaa gtatatttga tatataaagt atactgttta
     6001 acgtttgata gaaaagatgc tatactagaa gtagtaactt ttagaagtag gatgtaatgg
     6061 caaaatctaa taccaataag cgctatgcat tacgtaaact cggaaagatt tttggttctg
     6121 cggtaatcgg gctaacttta tttttaggag gaatctgaat acaagttttg ttcatgctgc
     6181 tgatgggatt caatatgtca gagatgatac tagagataaa gaagagggaa tagagtatga
     6241 tgacgctgac aatggggata ttattgtaaa agtagcgact aaacctaagg tagtaaccaa
     6301 gaaaatttca agtacgcgaa ttcgttatga aaaagatgaa acaaaagacc gtagtgaaaa
     6361 tcctgttaca attgatggag aggatggcta tgtaactacg acaaggacct acgatgttaa
     6421 tccagagact ggttatgtta ccgaacaggt tactgttgat agaaaagaag ccacggatac
     6481 agttatcaaa gttccagcta aaagcaaggt tgaagaagtt cttgttccat ttgctactaa
     6541 atatgaagca gacaatgacc tttctgcagg acaggagcaa gagattactc taggaaagaa
     6601 tgggaaaaca gttacaacga taacttataa tgtagatgga aagagtggac aagtaactga
     6661 gagtacttta agtcaaaaaa aagactctca aacaagagtt gttaaaaaaa gaaccaagcc
     6721 ccaagttctt gtccaagaaa ttccaatcga aacagaatat ctcgatggcc caactcttga
     6781 taaaagtcaa gaagtagaag aagtaggaga aattggtaaa ttactcttac tacaatctat
     6841 actgtagatg aacgtgatgg aacaattgaa gaaactactt ctcgtcaaat tactaaagag
     6901 atggtaaaaa gacgtataag gagagggacg agagaacctg aaaaagttgt tgttcctgag
     6961 caatcatcta ttccttcgta tcctgtatct gttacatcta accaaggaac agatgtagca
     7021 gtagaaccag ctaaagcagt tgctccaaca acagactgga aacaagaaaa tggtatgtgg
     7081 tatttttata atactgatgg ttccatggca acaggttggg tacaagttaa tagttcatgg
     7141 tactacctca acagcaacgg ttctatgaaa gtcaatcaat ggttccaagt tggtggtaaa
     7201 tggtattatg taaatgcatc gggtgagtta gcggtcaata caagtataga tggctataga
     7261 gtcaatgata atggtgaatg ggtgcgttaa cagattcctc cctgcgacaa aattgataat
     7321 agtatgtctg aatatataat tatatataaa aagccataga taaggtcatc ttatagcgac
     7381 tataaaaaag tgaacaagcc tagtcttctg tacattagaa ttcagatttg ttcacttttt
     7441 gtttaatact caatgaaaat caaagagcaa actagaaaac tagtcgcagg ttgctcaaag
     7501 tactgctttg aggttgtaga tagaactgac gaagtcagtc acatatataa tccaaggtga
     7561 cgttgacgcg gtttgaagag atttttgaag agtataagtc tttgattttg agactaagga
     7621 gagtcagggt caaagtagtg aatgattctg aaataactca taatagtctc ttgagaggac
     7681 agataagagt aacaaatatg gtgctactca tgtgtcttgc attttgattt acaaacagcg
     7741 aagaaaatat aggaagctga aataaaatat agatgaatca atttggagag tcaacgcaat
     7801 ctatggcaat attttcgaag ctagggggaa gcattttaat ttcaatgtac taggcgtctt
     7861 ctacagctag ctagagtttc aattgtagat gttctttgaa ataatatctt agcgatttat
     7921 tggtattaga agaattagag aaattctttc tcaataaata aacatggcgt caccaaaact
     7981 gaagaagcgg tagtgttctt ggatggcatg gtggtaggca tctaagacta attcacggcc
     8041 tgcaaaggca gaaaccaaca tgaccagagt tgattttggc agatggaagt tggttgagaa
     8101 ggcatccacg accttccact catacccagg tttgataaag atattggtcc aaccagaatc
     8161 tgcttggatt tgcccatcaa acttggaacc aatagtttcc aaggtgcgga tagaagtggt
     8221 tccgacagcg atgacacgac caccattttt tttgacagag cgaagggtgg cagcagcttc
     8281 ctcagaaagt tgatagaact ctgagtgcat ttcgtgttcg tccagattat ccacagaaac
     8341 aggtctaaag gttccgagtc cgacatggag agtcagatag actagatgaa cacccttagc
     8401 ttggatttct gccagcagtt ctttggtgaa gtgaagacca gcagtcggtg ctgcagcaga
     8461 gccgctttcc ttggcgtaga cggtttgata acgttcacgg tcatctaatt tttcgtggat
     8521 ataaggtggc agaggcattt ctcccagact ttccaagact tctaggaaaa ttccttggta
     8581 ttcaaagcgg acaatgcgtc ccccgtgggt caattcttct gtaacgacag cgctgaggcg
     8641 gccatcacca aagctgatac gagtaccgac cttgaggcgt ttggcaggtt tagccagaac
     8701 ttcccactcg tctccactag tgttcttaag gaggagaagt tccacatgac ctcctgtctc
     8761 cactttttga ccatagaggc gggcagggag aactcgggtg tcgttcatga caagggcatc
     8821 accaggttcc agcatattaa taatagagtg gaagtgttta tcttgcattt ctcctgtctc
     8881 acggttgacg atgaggagtt tggaggcatc acgtttttca aggggcgttt gggcaatcaa
     8941 ttcctctggc aagtggaaat caaaatcagc tgtattcata tatctgttca ttctttctaa
     9001 gttctaatcc tatccattat aacacgtttc aagtttggac gctctttttt tagaaattaa
     9061 atcgttttca cttgacaaaa attggtctat accatataat aaatatagac agaaatggag
     9121 gatgaaaaga tgaaagttat taaagttgaa aaccaagttc aaggtggaaa agttgctttt
     9181 gagattttga aggaaaaatt ggctaatgga gctcaaactc taggacttgc gacaggaagt
     9241 agcccacttg aattttacaa ggaaattgtt gagagtgacc ttgatttttc aaatctaacc
     9301 agtgtcaacc ttgatgagta tgtgggcctt gatggggaca atccacagtc ttatcgttac
     9361 ttcatgcaag aaaacttgtt caaccaaaaa ccatttaaag aaagtttctt gcctcgtggt
     9421 gttaaggaca atgctgaagc tgaagttgaa cgctacaacc aaattttggc tgaccatcca
     9481 gttgacctcc aaatcttggg aatcggtcgc aatgggcata tcggctttaa tgagcctggt
     9541 actccatttg acagtcaaac gcatctagta gaacttgacc agtctactat cgaagccaat
     9601 gcacgcttct ttgccaagat tgaagacgtc ccaacccaag ctatttcaat ggggattaaa
     9661 aacattttgg atgccaagtc tattattctt tttgcttacg gtgagtcgaa agcagaagcc
     9721 attgccggaa cagtatctgg cccagtgact gagaacctac ctgcaagtag cctccaaaat
     9781 caccctgatg taaccatcat cgcagatgct gaagcgctta gcttactcga aaaataaaaa
     9841 agcaatgttc tattgaacgc tttcaactct tgtataaatg gaagaaaaaa tcaaaattaa
     9901 accgcatttt tgcttgacaa ttattccttt tacgtgtaga atgaaataga tattgaactt
     9961 gaagggagtg aaaaaaatgt ctaaaacagt agtacgtaag aatgaatctc ttgacgacgc
    10021 acttcgccgt ttcaaacgtg cggttactaa agctggtact cttcaagaaa cacgcaaacg
    10081 tgaattctat gaaaaacctt ctgtaaaacg taaacgtaaa tcagaagtag ctcgtaaacg
    10141 taaaaaattc taattggaat gaaaggctag agaaatctag tcctttttct tttaaataaa
    10201 tactccaaag cctgcaaaaa tctgaaactt cctcctacaa tttgatataa tagagagaag
    10261 aactcatttg aaggaggaaa tgatgtcggt tttagtaaaa gaagtgattg aaaagcttag
    10321 actagacatt gtctatggtg aaccaaaatt gcttgaaaag gaaatcaata cagcggatat
    10381 tacgcgacct ggtcttgaaa tgacaggcta ttttgactac tatacaccag agcggattca
    10441 acttttgggg atgaaggagt ggtcttatct gatcagcatg ccttccaaca gccgttatga
    10501 agttttgaaa aaaatgtttc tacctgagac accagcagtc attgttgccc gtggtttggt
    10561 ggttccagag gagatgttaa aggctgctag agaatgtaag attgctattt taaccagccg
    10621 tgcagctacc agtcgtttat ttggagagtt atctagctat ctggattctc gtttggcaga
    10681 acgtaccagt gtgcacggtg tcttgatgga tatttatggg atgggcgtct tgattcaggg
    10741 agatagtgga attggtaaga gcgagacagg tcttgagctt gtcaaacgtg gtcaccgttt
    10801 ggtagccgat gaccgtgtcg atatctttgc caaggatgag attactctct ggggtgaacc
    10861 agctgaaatt ttgaaacact tgattgaaat tcgtggggtt ggtattatcg atgttatgag
    10921 tctctacggt gcgagtgctg tcaaggattc ttcacaggtt cagcttgctg tctatttgga
    10981 aaattacgat acgcataaga cctttgatcg tcttggaaac aatgcagagg aacttgaagt
    11041 ttctggcgta gccattcctc gtattcgtat tccagttaaa acaggtcgta atatctctgt
    11101 tgtgattgag gcagctgcca tgaattatcg tgccaaggaa atgggctttg atgcgacccg
    11161 tttgttcgac gaacgactga caagtctcat cgctcgaaat gaggtgcaaa atgcttgatc
    11221 caattgctat tcaactagga cccctagcca ttcgttggta tgccttatgt attgtgacag
    11281 gcttgattct tgcggtttat ttgaccatga aagaagcacc tagaaagaag atcataccag
    11341 acgatatttt agattttatc ttagtagctt ttcccttggc tattttagga gctcgtctct
    11401 actatgttat tttccgattt gattactata gtcagaattt aggagagatt tttgccattt
    11461 ggaatggtgg tttggccatt tacggtggtt tgataactgg ggctcttgtg ctctatatct
    11521 ttgctgaccg taaactcatc aatacttggg attttctaga tattgcggcg cctagcgtta
    11581 tgattgctca aagtttgggg cgttggggta atttctttaa ccaagaagct tatggtgcaa
    11641 cagtggataa tctggattat ctacctggct ttatccgtga ccagatgtat attgagggga
    11701 gctaccgtca accgactttc ctttatgagt ctctatggaa tctgcttggc tttgccttga
    11761 ttctgatttt tagacggaaa tggaagagtc tcagacgagg tcatatcacg gccttttact
    11821 tgatttggta tggtttcggt cgtatggtca tcgaaggtat gcgaacagat agtctcatgt
    11881 tcttcggcct tcgagtgtcc caatggctgt cagttgtcct tatcggtctc ggtataatga
    11941 tcgttattta tcaaaatcga aagaaggccc cttactatat tacagaggag gaaaactaaa
    12001 tgttagaagt tgcatatatt cttgttgccc tagctttgat tgtctttttg gtctatctga
    12061 tcattactgt acaaaagctt ggtcgtgtca tcgatgaaac agaaaagacg attaaaacct
    12121 tgacttcaga tgtggatgtg accttgcatc acaccaatga gttgttggct aaggtcaatg
    12181 tcttggcaga tgatatcaat gtcaaggtgg ctacgattga tccactcttc agtgctgttg
    12241 cagatttatc tctatctgtt tcagacctca atgaccatgc gcgtgtcttg agcaagaaag
    12301 cttcatcagc tggttcaaaa acactcaaga ctggtgcaag tctgtcagct cttcgtcttg
    12361 caagtaaatt tttcaaaaaa taaaaaagga gaatccttat gggtaaatta tcctcaatcc
    12421 ttttaggaac cgtttcaggt gcagctcttg ccttgttttt aacaagtgat aagggcaaac
    12481 aagtttgcag tcaggctcaa gattttctag atgatttgag agaagatccg gagtatgcca
    12541 aggagcaagt ctgtgaaaaa ctgacagaag ttaaggagca ggctacagat tttgttctga
    12601 aaacaaaaga acaggttgag tcaggtgaaa tcactgtgga cagtatactt gctcaagcta
    12661 aatcctatgc tcttcaagcg acagaagcat caaaaaatca attaaataat ctcaaggagc
    12721 aatggcaaga aaaagccgaa gctcttgatg actcagaaga gattgtgatt gatataacag
    12781 aagaataaac catcaccatc tccggacgga ctatgtatct ggggatggtg atttttatct
    12841 ggaatctagt ctttgtggta taataattac tatgcagaaa aaaccaacgt cagcctatgt
    12901 gcacatccca ttttgtaccc agatttgtta ttattgtgat ttttcaaagg tcttcatcaa
    12961 aaatcagcca gtcgacagct atttagagca tctgctggaa gagtttcgtt cttatgatat
    13021 tgaaaagttg tcaacccttt atatcggtgg tggaacaccg acagccctgt cggctccgca
    13081 actggaggtt ttactgaatg gcttgactaa aaacttggac ttgtctgtct tggaagagtt
    13141 gaccattgaa gccaatccag gcgatttgga tgcggataag atagctgttt tgaaaaattc
    13201 ggctgtcaat cgtgtttcgc taggtgtcca gacctttgat gataagatgt tgaaaaagat
    13261 tgggcgcagt catttggaga aggatattta tgaaaatatc gatcgcctga aactggctgg
    13321 ttttgacaac atctccattg atttgattta tgctctgcct ggtcagacca tggagcaagt
    13381 aaaggaaaat gtggctaaag ccattggact ggatattccc cacatgagtt tgtatagttt
    13441 gattttagaa aaccatacgg tctttatgaa ccggatgcga cgtgggaaat tgcctctgcc
    13501 taaggaggaa ctagaagcgg agatgtttga gtacatcatt gcagagctgg agcgagcggg
    13561 ttttgagcat tatgagattt ctaatttctc caaacccggc tttgaaagtc gtcataatct
    13621 catgtactgg gacaatgctg aatactatgg tattggtgca ggggcatctg gttatgtcaa
    13681 tggagtacgt tataaaaatc atggtcccat tcgtcattat ctcagtgcgg ttgaggaagg
    13741 caatgcttgt attacagaag atcacctgag tcaaaaggag caaatggaag aagaaatgtt
    13801 cttgggactc cgcaagaaat ccggggtttc catggcgcga tttgaggaaa aatttggaca
    13861 gtcttttgct ggactttatg gagaaattgt cagagatttg gttcaacaag gcctcatgca
    13921 gattgagggt gaccacgttc gcatgacaaa gagaggtctc tttttgggag atactgtagc
    13981 agaacgattt attttggagt aggatcatgg gcttaactta tcaaatgaaa atgaaaattc
    14041 cttttgatat ggctgatatg aacggtcata tcaaacttcc agatgtgatt ttgctgtcac
    14101 tgcaagtttc agggatgcag tcgattgaac tgggagttag tgataaggcc attttggaag
    14161 actataatct ggtctggatt atcgcagaat atgatattga ggtggttcgt ttgcctcgtt
    14221 ttgcggaaga aattaccatc gaaacggaag ccttgagcta caatcgactt ttttgctacc
    14281 gtcgctttac tatttataat gaagcggggc aggagctcat ccatatgatg gcgacctttg
    14341 ttctcatgga ccgcgacagt cgaaaagtcc atgctgttga acctgagatt gtggctcctt
    14401 accagtctga ttttgataaa aaacttatcc gtggaccgaa gtatgagtcc ttggaagagc
    14461 cgatcagcaa ggattaccat gttcgttttt acgacttgga tatgaatggt catgtcaata
    14521 acagtaaata cttggactgg atttttgagg tcatgggagc ggattttttg acccactata
    14581 ttcccaagaa aatcaacctc aagtatgtca aggaagttcg accaggtggg gtgattacat
    14641 cggctgttga acggactgga ctggaaagca agcatgaaat tacaagtgat ggggttatca
    14701 atgcccaagc aatcattact tggaaagaaa taaaaaaggc ttagggagaa atagatgaaa
    14761 tataaaggct atttaattga tttagacgga accatttata aggggaaaga ccgaatccct
    14821 gctggagaga cttttgttca tgaattgcaa aagcgggaca ttccctatct ctttgtaacc
    14881 aacaatacaa ctcgcactcc agagagtgtt aaggagatgt tggctcagaa ttttaatatc
    14941 gatacgcccc tatcgactgt ctacacagcg actttggcaa ccatcgacta tatgaatgat
    15001 ttggggcttg aaaagaccgt ctatgtcgtc ggagaagcag gactcaagga agccatcaag
    15061 gcggctggtt atgtggaaga caaggaaaac cctgcctacg tggtagttgg tctggattgg
    15121 caagtcgact atgaaaaatt tgccacagca actcttgcta ttcaaaaggg tgcccatttt
    15181 atcggaacca atcctgacct caacatcccg acggaacgtg gtcttttgcc aggtgctggc
    15241 tccctgatta cgctgcttga agtagcgaca cgagtgaagc cggtttatat cggaaaaccc
    15301 aatgccatca ttatggacaa ggcggttgag cacttaggtt tggaacgtga agagttaatt
    15361 atggttgggg acaattattt gactgatatc cgggctggga ttgacaatgg cattccaacg
    15421 ctcttggtga cgacaggttt tactaaggca gaagaagtag ctggcctgcc aatcgcaccg
    15481 acacatgtgg tttctagcct tgcggagtgg gattttgatg aaaactaaat tgatcttttg
    15541 gggctctatg ctctttctcc tctccctctc aatccttctg accatttatc tggcttggat
    15601 tttctatcct atggagattc agtggctaaa cttaacgaat cgagtctatc taaaaccaga
    15661 aaccattcaa tacaattttc atatcttgat gaattatctg accaatcctt ttagtcaggt
    15721 cttacagatg cctgattttc gttcgtcagc agctggtctg caccatttcg cagtggtcaa
    15781 gaatctcttt catttggttc agctagtagc tctagtgaca ctgccaagtt tctatgtctt
    15841 tgtcaataga attgtgaaaa aggacttttt gtctctttat cgaaaaagtc tcctgactct
    15901 agtagtctta cctgtgatga ttggacttgg gggagttttg attggttttg accaattctt
    15961 tactcttttc catcaaattc tctttgtggg agatgatacc tggctttttg atccagccaa
    16021 ggatcctgtt attatgattt tgccagagac cttctttctt catgccttcc tcctcttttt
    16081 tgccctctat gaaagcttct ttggctatct gtgtctgaaa agtcgtagga aatgaaatga
    16141 aagatgtttt taatttgcat atataaaaat atatatcgtt taaaaccatc gttaagagtg
    16201 aattaaccaa atccaattgt gtcaatcgtt cgcaaaaagc ctatttttct tgaaaaatag
    16261 gctttttttt ctaaaaatcc ctagtcaagc gctttatttt tttgtataat agaagtagca
    16321 aagtatagat aagaagagaa aagcatgatt acactatttc tatcaccgag ctgtacatca
    16381 tgtcgtaagg ctaaggcctg gttagaaaaa cataaggttc cctttgtgga acacaatatt
    16441 atgaccagtc ctttaacaag aaaagaattg caacacatcc tttccttgac cgaaaatggt
    16501 actgatgaca tcatttcaac tcgttcaaaa atttttcaaa aattgaatat tgatgtagag
    16561 agtatttcgg tatcggaatt gcttcatttg attgagcagt atcctagtct tttgcgtcgt
    16621 ccaattatta ttgatgccaa gcgtatgcaa atcggtttta atgaagatga gattcgtgct
    16681 tttctccctc gtagttatcg taagcaagaa ctaaaagaag caagaatgag agctggtatt
    16741 agttaatgaa caaacagtat agttacccac tagatttgtc gtggagcact gaagaacttg
    16801 cttcagtgct ttcttttttt aatgatgttg aagctgccta tgaaggcaaa gtagaggcta
    16861 aaaagttact ggactcctat aagggattca aagcggtcgt gccaagcaag agcgaagaga
    16921 aacgtttggg acgagaattt gaaacggtta gtggctattc actttaccga gcagttcagg
    16981 ctgccaaaga aaaaggggaa ggaaagattt ctcttggaaa gtaaatttga atttgctaaa
    17041 gagcttgtta agaaagcagg tcagtacatt cttgaccata tgcaggaaga cttgcgtgtt
    17101 gaaaccaagt cctctccaac agatttggtg accagactgg acaaagaagt tcaggaactc
    17161 ttggttggtg agattttgtc ccgttatcct gaggataaga tttgtgctga agaaggttgt
    17221 ctgcgagcct cggttcaaga gggcaaggtt tgggtcattg atcccattga tggtaccaat
    17281 aattttgtag cccagcagga agattttgct gttatgatgg cttattttga aaatggtcag
    17341 ggacagtttg gtctgattta tgatgtggtc aaaggggatt gttaccacgg tggtggggaa
    17401 tttccagttt gtctaaatga taggccccta gcttctttta aaactaaacc acttggagat
    17461 tttctcattg cagggaatag tggtatgctg gaaaccaatg aatgggggct ggctgatttg
    17521 agccgagcgg ttcttggtgt tcgtgtctat ggaagtgcgg ccattagttt tgccaagatt
    17581 ttgtcagggc gtttgttgac ctatctcacc tatctgcaac catgggatta tgccgcagct
    17641 agtattttag gggaaagtct gggctatcgg gttgtgaccc tttttggtga agctcctgat
    17701 tttcaaacca gacagccggt catgatggtg cctcttgaga tgcaggagga aattcagtcc
    17761 tatatttacg aaaggaaaag aacttaaatg aaatttccag aaggatttgt tgaaaaatat
    17821 aaagaaatac taggagatga ggcaagagat tttcttgcct cttttgagga ggaagcggtt
    17881 tcggcctttc gggtcaatcc cttaaaagaa gagcaacttt ccttttctga tgccattact
    17941 caaacccctt ggggccacta tgggaaggtt tcagggaaat cgcctgagca tgctacaggt
    18001 ttagtttatt cgcaagaacc tgctgctcaa atggtggctc aagtagccca acccagtcct
    18061 ggtatgaagg tcttggactt ggctgctgca ccggggggca aatcaactca actggcagcc
    18121 tatctagcag gggaggggct acttgtttcc aatgaaattt caagcaaacg ggctaagatt
    18181 ttggttgaaa acatggagcg ttttggagcg acaaatgtcg tggtgaccaa tgaatctgcc
    18241 gaccgcttgg tcaaggtctt taaaggctat tttgacttaa tcgttcttga tgccccttgc
    18301 tctggtgaag gaatgtttcg taagcaacct gatgctatgg actattggag cttagattat
    18361 ccgagtcaat gtgctagctt gcaaagagaa attctggagg atgcagtgac catgttagct
    18421 gaaggtggtc gtctggttta ttcgacctgt acctgggcac ccgaggaaaa cgaagagatt
    18481 gtcaattggt tgctggaaga gtatgatttt gacttgttgc cagtagagca tatcaatgga
    18541 atggtagctg gtattgacct gccagaaacg gctcggatgt accctcatca gtttaaggga
    18601 gagggtcagt ttgttgccca tctacagttt aagggtaaca atccagctcc taaatttaag
    18661 gcaagtaaga gtaacctcag ccgtgaacaa gttgccttgt ggcaggaatt tgcccaaaac
    18721 catttgaagg tcaatctacc gggtatcttg cagatttttg gagaccagct ttatctcttg
    18781 ccagaacttt tgccagattt agggaagctc aagattgctc gcaatggact gcatctgggt
    18841 acttttaaga agaaacgctt tgagcccagt ttcgctcttg gtttagcctt gaaaccgagt
    18901 caggtcgaac agtcggttga aattggccaa gaagagtttg tcaagtatgc ggctggagaa
    18961 accgttcagc tggcagaaag tctgccaaat ggttggtacc aagttttggt taaaggcaat
    19021 ggcttgggct ttgcaaaggt tactggaaat gttttgaaaa attattttcc aaaaggcctc
    19081 agattcaaat gaaaatgcta gtcaaatatc ttattctatg ttatagtgaa agtatggatt
    19141 tttttagtag gatgattctt gatatcttga ttgccatcac atgtagtttt actactcgat
    19201 gaaaatcaaa gagcaaacta ggaagctagc catagatagc tcaaaacact gctttgaaga
    19261 gattttcaaa gagtacaaga agtttagtta ttagcgttct taccgcttgt aaactagatt
    19321 tctcataaaa tagaatcttt tccttttagt tgtaaactag tctgggagag tagagagatt
    19381 tgagatacct ttctagcttt tagattatca tctaaggaga gtaatttccc ttgcattaaa
    19441 aaggggaaaa agagacacga aatgactata atgggtgaca atgggggaag ggatagacaa
    19501 gagattttat ccacatatga aaaaaggagg ttaggaaaga gtaatatagc ctatattata
    19561 taaataatca attgcgcaga aatttggtaa gaattcatgc gtcaactcat aaagaactac
    19621 ttaaaaaatt cacagtattc ataattattt tcgaggagaa aaacagtgaa aaaaagaaaa
    19681 aagcttgctc tgtctcttat cgctttttgg ctgacggctt gtttagtagg ctgtgctagc
    19741 tggattgatc gtggagaatc cataacggct gttggctcaa ctgccttgca acccttggtt
    19801 gaagtagcgg cagatgaatt tggcacgatc catgttggaa aaacggtcaa tgtccaaggg
    19861 ggaggttctg gtacaggctt gtcccaggtt cagtctgggg cagttgatat aggaaactca
    19921 gatgtatttg ctgaggaaaa agacggaatt gatgcttctg ctcttgttga ccacaaggtc
    19981 gcggtagctg gcttggctct gattgtcaat aaggaggttg atgttgataa cctaacgaca
    20041 gagcaacttc gtcaaatctt cataggtgag gtaaccaatt ggaaagaggt tggtggtaag
    20101 gacttaccca tctctgttat caatcgggca gccggctctg gttctcgtgc tacctttgat
    20161 actgtcatta tggaaggtca gtctgccatg caaagtcagg agcaggattc aaatggagcg
    20221 gtaaaatcaa tcgtatcaaa aagtccagga gctatctctt atttatctct tacctatata
    20281 gatgattcgg tcaaaagcat gaagttgaat ggctatgact taagtccaga aaatataagt
    20341 agcaataatt ggcccttgtg gtcttatgag catatgtata cattggggca gcccaatgag
    20401 ttggctgcag aatttctcaa ttttgttctc tcggatgaga cccaagaagg gattgtcaaa
    20461 ggattgaagt atattccgat taaggaaatg aaggttgaaa aagatgctgc cggaactgtg
    20521 acagtgttgg aagggagaca ataatgaatc aagaagaatt agctaagaaa atgttgcttc
    20581 catcaaagaa ttctcgtctg gagaaattag gaaaaggttt gacctttgcc tgtctttctt
    20641 tgatagtcat ccttgtggcc atgattttgg ttttcgtagc gcaaaaaggc ttgtcgacct
    20701 tctttgtcaa tggtgtgaat atctttgact ttcttttggg aggaacttgg aatccttcta
    20761 gtaaagaatt tggtgccctt cctatgattt tgggttcctt tatcgttacc attctctcag
    20821 cccttatcgc aacacccttt gctattggtg cagcagtttt tatgaccgaa gtatcaccaa
    20881 aaggggcgaa gattttgcaa ccagctattg aactcctggt tgggattcct tcagtagtgt
    20941 acggatttat tggcttgcaa gtcgtcgttc cctttgttcg cagtgtcttt ggtgggactg
    21001 gttttgggat tttgtcaggg atttccgtcc tctttgtcat gattttgccg accgtaacct
    21061 ttatgacaac ggatagcttg cgtgcggttc ctcgttatta tcgtgaggcc agtttcgcta
    21121 tgggagccac tcgctggcag actatctggc gtgtgatctt gaaggcggcc cgttctggta
    21181 ttttcactgc agtggtcttt gggatggcgc gtgcctttgg tgaggcttta gctatccaga
    21241 tggttgtcgg aaactcagct gttatcccaa cttccttgac cacaccagct gcaactttaa
    21301 cttctatatt aactatggga attgggaaca ctgtcatggg aactgtaaat aataatgttc
    21361 tctggtcact ggccttggta ctgctcttga tgagtttagt ttttaacagt gtgattaaat
    21421 tgattacgaa agaaagagga aagaaaaatt atgcgcgcta agaaattaga taaacttgca
    21481 acagctgtcc tctatacgat tgctagcatc attgtgacaa tcttggcttc cttaattctc
    21541 tatatcttgg ttcggggctt gccccatatc tcttggtctt tcttgactgg aaggtcttct
    21601 gcttttcaag caggtggtgg gattggcatt cagctttaca attccttttt cctattggtc
    21661 attaccttga ttatttctgt acctctttct atgggagctg ggatttactt ggctgaatat
    21721 gctaaaaaag gtcctgttac caactttgtg cggacttgta ttgaaatctt gtcctcttta
    21781 ccatcagtgg tggtgggtct ctttggttac ttgatctttg tagtccagtt tgagtatggg
    21841 ttttcaatca tttcaggtgc cttggccttg acagtcttta acttgcctca gatgacgcgt
    21901 aatgtagagg atagtttgaa acacgttcac catacccaac gtgaggctgg tctggctctt
    21961 gggatttctc gctgggagac agtggttcat gttgttattc cagaagcgct tccaggtatt
    22021 gtaacgggtg tcgtcttggc atctggtcgt atctttggcg aagctgcagc tctgatctat
    22081 acagcagggc aatcggcgcc agctcttgac tggtctaact ggaatatcct cagtgtgact
    22141 agccccatct ctatcttccg tcaagcagaa accttggctg tccatatctg gaaagtcaat
    22201 agtgaaggca ctattccaga tggaaccatt gtatcagcag gttctgccgc tgtgctcctg
    22261 atctttatcc tgatttttaa ctttggagct cgtaagttcg gaagctatct acacaagaaa
    22321 ttaaccgctg cctaaaggag aagccatgtc aacatataac tgggatgaga agcatatcct
    22381 tacctttcct gaagaaaaag tagccctttc tactaaggat gtccatgttt actatggtaa
    22441 aaatgaatcc attaagggga ttgatatgca atttgaaaga aataaaatta cagctttgat
    22501 tggtccgtcg ggatcgggga aatctaccta cttacgcagt ctcaatcgca tgaatgatac
    22561 cattgatatt gctaaagtaa ctgggcagat tctctatcgt ggaattgatg tcaaccgtcc
    22621 agaaatcaac gtttatgaaa tgcgtaaaca cattggaatg gtttttcaac gccccaatcc
    22681 atttgctaag tcaatttacc gtaatattac ctttgcgcat gaacgtgctg gagttaagga
    22741 taagcaagtc ctagatgaaa tcgtagaaac ctcccttcgt caggctgccc tctgggatca
    22801 ggttaaagac gatctccaca agtcagcctt gaccttatca ggtggtcagc aacaacgtct
    22861 ctgtatcgct cgtgccatct ctgttaagcc agatatcctc ttaatggatg agccagcctc
    22921 agccttggat ccgattgcga ccatgcaact agaagagacc atgtttgagc tcaagaaaaa
    22981 ctttaccatc atcattgtaa cgcataatat gcagcaggct gctcgtgcaa gtgactatac
    23041 aggcttcttt tacttgggtg atttgattga gtatgacaag actgcaacta ttttccaaaa
    23101 tgccaagcta cagtccacca atgactatgt atctggtcac tttggttaga aaggaaaccg
    23161 tatgacagat gcgattttac aggtatcaga cctgtccgtt tattataata aaaagaaggc
    23221 tttgaatagt gtttccctat ctttccaacc taaggaaatt acagccttga ttggtccatc
    23281 tggatcaggg aagtcaaccc tcctcaagtc tctcaaccgc atgggagatc tcaatccaga
    23341 ggtgaccaca actggatccg tggtgtacaa tggtcacaac atctacagtc cgcgtacaga
    23401 tacggttgaa ttacgtaagg aaatcggaat ggttttccaa caacctaatc ctttccctat
    23461 gactatctat gagaatgttg tctacgggct tcgtatcaat ggaattaagg ataagcaggt
    23521 tctggatgaa gccgtagaaa aagccttgca aggtgcctct atctgggatg aggtcaagga
    23581 tcgtctatat gattcagcta ttggattgtc aggtggtcaa cagcagcgtg tctgcgtggc
    23641 ccgtgtcttg gcaactagtc ctaaaatcat cctcttggat gagccaactt cggctttgga
    23701 tccgatttca gctggtaaaa ttgaggaaac cttgtatggt ctaaaagaca agtacaccat
    23761 gcttctggta acccgttcca tgcagcaagc ttcacgtatc tctgataaga caggattttt
    23821 cctagatgga gatttgattg aatttaatga taccaagcag atgttccttg atccccaaca
    23881 caaggaaacg gaagactata ttacaggaaa atttggataa ggagatgaaa gatgttacga
    23941 tctcaatttg aagaagattt agagaaatta cataaccagt tctacgctat gggacaagaa
    24001 gtgctctcac aaatcaatcg tacggtacgt gcttttgtca cgcatgaccg tgacctggca
    24061 aaagaggtca tcgaagatga tgcagaagta aatgaatacg aagtgaaact ggaaaagaaa
    24121 tcatttgaaa tgatcgcact ccaacaacca gtctctcaag atttgcgtac agtcttgact
    24181 gtccttaagg ctgtatcaga tgtggagcgt atgggggatc acgctgtagc cattgcccag
    24241 gcaaccatcc gtatgaaggg ggaagaacgt attccagctg tagaggaaga aattaaaaaa
    24301 atgggacgtg aagttaaaag cgttgttgaa gcagcacttg atctttatct taatggttct
    24361 gttgacgacg cataccgggt ggcctccatg gatgagcaaa ttaaccacta ttttgaaact
    24421 atccgtgacc ttgcgactga agagattaag aaaaatccag aagccattgt tacgggtcgt
    24481 gattatttcc aagttatttc ctacttggag cgtatcggtg actatgctaa aaatatctgt
    24541 gaatgggttg tctactttga aacaggtaag attgtcgaac tataaaatag tttaattctg
    24601 tataaaaagg agcttgaaat caactgatgg ctcctttttt tgaacgaaaa aatatttttg
    24661 agataaaaat ttaaaaaaca cttgacaagt aacttggata tcgttataat tatacaaaat
    24721 taacagagag gttgtttatt tatgaaatca aaaaaatgga tatttgtttt atgtaatttt
    24781 cttgcaagtt tcttcttagt agcttgccag tcaggttcta atggttctca gtctgctgtg
    24841 gatgctatca aacaaaaagg gaaattagtt gtggcaacca gtcctgacta tgcacccttt
    24901 gaatttcaat cattggttga tggaaagaac caggtagtcg gtgcagacat cgacatggct
    24961 caggctatcg ctgatgaact tggggttaag ttggaaatct taagcatgag ttttgacaat
    25021 gttttgacca gtcttcaaac tggtaaggct gacctagcag ttgcaggaat tagtgctact
    25081 gacgagagaa aagaagtctt tgatttttca atcccatact atgaaaacaa gattagtttc
    25141 ttggttcgta aggctgatgt ggaaaaatac aaggatttaa ctagcctaga aagtgctaat
    25201 attgcagccc aaaaagggac tgttccagaa tcaatggtca aggaacaatt gccaaaagct
    25261 caattaactt ccctaactaa tatgggtgaa gcagtcaatg aattgcaggc tggaaaaata
    25321 gatgctgttc atatggatga gcctgttgca cttagttatg ctgctaaaaa cgctggctta
    25381 gctgtcgcaa ctgtcagctt gaagatgaag gacggcgacg ccaatgccgt tgctcttaga
    25441 aaaaatagtg atgatttgaa agaagtggta gacaaggtca tccaaaaact taaggatgaa
    25501 ggaacttacc aaagctatct tgaaaaagca gcaagtttaa ctgaagttga agaataagaa
    25561 aaagcagagt tggatgtcat tccaattctg cttttaaata gtttaaaaca ttttccagat
    25621 tgtctaggga caccttggca aattgataag gacaggtgct taaataggcc tcttcaaaga
    25681 agtccaagcc cagttgactg tgtttgagac tggcgatttt aggatactgt ctcaggtcta
    25741 gcaataaacc atcgtgtagg ggataatgag gaaggggaca aggtgtttta gttatccttt
    25801 cctcgatttg tttttggtaa gattcatgat tggtcaagcg agccaaacga tttttttcaa
    25861 acaattgact gtcaatatag agtgacaggg ccttttgcat gatgaggttg ctgtcgtagt
    25921 ctaggatatt tttgtaggcc acgaaggctt ctttgatagt atttggaaga atgagtgctg
    25981 ttatccgcaa ggctggaaag aggctggttg agaaggattt gatataaatg acgcgttcct
    26041 ctgtatctag atagtggaag gtctggccct ttttagagtc caaatccccc agatagtcgt
    26101 cttcgacgat ataaacacta tatttggctg ctaagtcaag aatagctcgt ttgtcctgct
    26161 cagaatagga atggcctagg ggatagtgaa atcgaggaat ggtgtagaaa aacttaattt
    26221 ttcctgtttt gaagtggcct tccagctcct ccaagtcaat cccatcaatg cctcgttcaa
    26281 tcgtttgata gtccagcccc tgtgcaatca agaggcgatt catccgatgg taggttggct
    26341 gttccaccaa gatttccttg gcttggctag gaaaggatat ttgagagagg ataaacaagg
    26401 cttgttgggt tccagaagtc agtactagtt ggttagcctt gcagtagaga gcttggtcaa
    26461 agaggagttt gtgaatggat tgtcttaggt cttctaatcc ttcttgattg tcatagtagt
    26521 tgaagaggta gttttctcgg ccaatcaagg tttcattgac acagagtcgg aaatcgtcat
    26581 aggcactggc atgttcgtcg gtaacctcga tttctaggtc ttgatgttgc ccttgttcta
    26641 atacatagta gccactctga ggcttggcat agaggtattg ttcgtgccgt aattccagca
    26701 gggctcgttg aatggtgtcc ttgctgcagt gaaagtcaag gctcagttga cggatagaag
    26761 gcaggcgact acccgtcgga aatcgtccag actcgatacc atttttcaga taggaaacga
    26821 cctctttgta cttgctttgt ttcttcattc cagacctccc ttgattttgt tacattgtac
    26881 cctttttttt ccttcttggc aatgtctaga gcgtttctct cgttcacatc taggggcaaa
    26941 tgtggtatac ttaggagtaa gatttataga ataacagaca agaaagtgaa tggataagaa
    27001 cgtgcaggaa ccagtgaaat tatttcaata caatactctt ggagccttga tggctggcct
    27061 ttatggtggt actatgacag taggagaatt gctagaacat ggtgaccttg gtttgggaac
    27121 cttggattcc attgatgggg aattgattgt cttggatggc aaggcttatc aggccaaaga
    27181 gtcaggagac cagccagaaa ttgtggaagt gtcaccagat gcccttattc cttacgctgc
    27241 agtggtaccg catcaggcag aggtcatttt ccgccagcgc tttgagatga cagacaagga
    27301 attgaaagaa cgaattgagt cttattatga tggggaaaat cttttccgct ctatcaagat
    27361 tcggggggaa ttttcacata tgcatgtgcg tatgattcct aagtcaacac ccgataccaa
    27421 gtttgctgat atcgcaaccc atcaaccgga atatagtcgt gacaatgttg cggggactat
    27481 tgttggtttc tggacgcctg agattttcca tggggtcagt gtggcaggct accatctgca
    27541 cttcatatca gatgatttga ccttcggtgg acatgtcatg gattttgtca tcaaggaagg
    27601 cattatcgag gtgggagcag ttgaccagtt ggaccaacgt ttcccagtcc aagaccgtca
    27661 atacttgttt gctaagttca atgttgacga gatgaaaaaa gatattgaaa aggcagaata
    27721 ggagaagaaa atgaccattc atatcattat taccatgttg ctgttgctag cttttctgat
    27781 aggaagcatt tggtttgcca aaaagaaata ccagattaat ctagctgtct tgggcttggg
    27841 tgcagtcgct ttctttgtct cttcgcagat tttggaaaaa ctggtgcata tcttgatttt
    27901 gcatcctcaa aaagacggta gtattgccct cttgcaagat catccgctta tctatattat
    27961 ctatggccta gccatggcag cattttttga ggaaacggct cgtcttgttt tcttcaaatg
    28021 gttggagaaa aagagaagtt tggaaaaggc agatgccttg gcctatggtc tagggcatgg
    28081 tggcttggag ttgatttttc taggccttac tagtttactc aatctttaca tcgttctttc
    28141 ggcagttcaa actcagaatc cacaggttat gcaattactg tctgaaaata tgttaaaaac
    28201 cattcagtca ctttcggtct ggcagattta tttgcttggt tttgagcgaa tcttggcgct
    28261 tggtttccaa ttacttttga cagtttgggt ttaccaagct gttcgccaga agaaatggat
    28321 ttatctccta gcagcctatg gcttgcatgc cttctttgat ctggcaccat ctcttttcca
    28381 agtaggctgg ttgacaaatc cagtcttggt tgaagtgatt ctagcactgg agctcgttct
    28441 ggttgcctat ggaaccaagg aaatcttttg taaaaaatca taaaaagggg ggaacctctt
    28501 tttcttatgc aaaatccaaa caaggtattt ttatggtcgt caaatgtctc taaaaatggt
    28561 ataatggaat gaattttgta aaaggaagag tgacatgtct gtaagagaaa aaatgcttga
    28621 aatcttagaa ggaattgata tccgttttaa ggaacccttg catagctata gttatacaaa
    28681 agtaggtgga gaggctgatt atttggtctt tccacgaaat cgttttgagt tggctcgcgt
    28741 tgtgaaattt gccaaccaag aaaatatccc ttggatggtt cttggcaatg caagcaatat
    28801 catcgttcgt gatggtggga ttcgtggatt tgtcatcttg tgtgacaagc tcaataacgt
    28861 ttctgttgat ggctatacca ttgaagcaga agctggggct aacttgattg aaacaactcg
    28921 cattgccctc cgtcatagtt tgactggctt tgagtttgct tgtggtattc caggaagcgt
    28981 tggcggtgct gtctttatga atgcgggtgc ctatggtggc gagattgctc acatattgca
    29041 gtcttgtaag gtcttgacca aggatggaga aatcgaaacc ctgtctgcta aagacttggc
    29101 ttttggttac cgccattcag ctattcagga gtctggtgca gttgtcttgt cagttaaatt
    29161 tgccctagcc ccaggaaccc atcaggttat caagcaggaa atggaccgct tgacgcacct
    29221 acgtgaactc aagcaacctt tggaataccc atcttgtggc tcggtcttta agcgtccagt
    29281 cgggcatttt gcaggtcagt taatttcaga agctggcttg aaaggctatc gtatcggtgg
    29341 cgtagaagtg tcagaaaagc atgcaggatt tatgatcaat gtcgcagatg gaacggccaa
    29401 agactacgag gacttgatcc aatcggttat cgaaaaagtc aaggaacact caggtattac
    29461 gcttgaaaga gaagtccgga tcttgggtga aagcaagtag gaagctgtat ttgaaaagct
    29521 gctgctatgt catggaaagg gggtgaaagc ctatcggtag cgaagatgta tgcaggtggt
    29581 tttactccct gcaagaggta gtggggacct gacagagccc cgatcggtta atctatgaaa
    29641 aagaaggaat ttatgccaat tgaaaaaacc aattatcgaa ttcaaaaacg tctctaaagt
    29701 ttttgaagac agcaacacca aggttctcaa agacatcaac tttgagttgg aagaagggaa
    29761 attctacacc cttctaggtg catctggttc ggggaaatca actatcctaa acattattgc
    29821 aggtttactg gatgcgacga caggagatat catgctagac ggtgttcgta tcaatgatat
    29881 tccaaccaac aagcgcgacg tacataccgt cttccaatcc tatgccttgt tcccacatat
    29941 gaatgtgttt gaaaatgttg cctttccact tcgcttgcgt aaaattgata agaaagaaat
    30001 cgagcagcgt gtagcggaag ttctcaagat ggttcagttg gaaggttatg aaaaacgttc
    30061 catccgcaaa ctttctggag gacaacgtca gcgtgtggcc atcgcccgtg ctatcatcaa
    30121 ccaaccccgt gtggtcttgt tggacgagcc tttatcagcg ctggacttga aattgagaac
    30181 agacatgcag tacgaattgc gtgaattaca acaacgattg ggcattacct ttgtctttgt
    30241 cactcacgat caggaagaag ctcttgccat gagtgactgg attttcgtta tgaatgatgg
    30301 cgagattgtc cagtctggaa cccctgtgga catctacgat gagccaatca accactttgt
    30361 tgccaccttt atcggggagt caaacatctt gccaggtacc atgattgagg actacttggt
    30421 cgaatttaac ggcaaacgct ttgaagcggt tgatggtggg atgaagccaa atgaacctgt
    30481 tgaggtcgtt attcgtccag aggacttgcg cattaccctt cctgaagaag gcaaactcca
    30541 agttaaggtc gacacccagc tcttccgtgg agttcattat gaaattatcg cctatgacga
    30601 acttggaaat gaatggatga tccactcaac ccgtaaggcc atcgtgggtg aggaaatcgg
    30661 tctggacttt gaatcagaag acatccacat catgcgtctc aatgaaaccg aagaagagtt
    30721 cgatgctcgt atcgaagaat acgtggaaat cgaagagcaa gaagcaggtc tgatcaatgc
    30781 gatcgaggag gaaagagatg aagaaaacaa gctctaaact ctttgtagtg ccctacatgc
    30841 tttggattgc cctctttgta ttggcaccct tggtcttgat tttcggacaa tcctttttca
    30901 acattgaagg ccagtttagt ttagaaaatt ataaatctta ctttgcgtca caaaacttga
    30961 cctacctcaa aatgagtttc aactcagtgc tttatgcagg gattgtgacc tttgtgacct
    31021 tgcttatcag ctatccaaca gccctctttt tgacccgtct caagcaccgt caactctggc
    31081 tcatgctgat tatccttcct acctggctca atttgctcct taaagcctat gcttttatcg
    31141 ggatttttgg tcaaaatggc tctattaacc aattcttgga atttatcgga attggttcac
    31201 aacagttgct ttttaccgat ttttccttta tctttgtcgc aagctacatc gagctcccct
    31261 ttatgatttt gccgattttc aatgtcttag acgatatgga taataatctc atcaatgcta
    31321 gttatgacct tggtgccact aagtgggaga ccttccgtca tgtcatcttc cctctatcta
    31381 tgaacggtgt gcgaagtggg gttcagtcgg tctttatccc aagtttgagt ctcttcatgc
    31441 tgacccgttt gattggtggg aaccgcgtta tcaccttggg gacggctatt gagcagaatt
    31501 ttctaaccaa tgacaactat ggtatgggtt caaccatcgg tgtgattctc atcctgacca
    31561 tgttcatcac catgtgggtg actaaggaaa ggagagaacg atgaaaaaat ttgccaacct
    31621 ttatctggga ctggtctttc tggtcctcta cctgcctatc ttttacttga ttggctatgc
    31681 ctttaatgct ggtgatgata tgaatagctt tacaggtttt agctggactc actttgaaac
    31741 catgtttgga gatgggagac tcatgctgat tttggctcag acatttttct tggccttcct
    31801 atcagccttg atagcgacca ttatcgggac ttttggtgcc atttacatct accagtctcg
    31861 taagaaatac caagaagcct ttctatcact caataatatc ctcatggttg cgcctgacgt
    31921 tatgattggt gctagcttct tgattctctt tacccaactc aagttttcac ttggcttttt
    31981 gaccgttcta tctagtcacg tggccttctc cattcctatc gtggtcttga tggtcttgcc
    32041 tcgactcaag gaaatgaatg gcgacatgat tcatgcggcc tatgacttgg gagctagtca
    32101 atttcagatg ttcaaggaaa tcatgcttcc ttacctgact ccgtctatca ttactggtta
    32161 tttcatggcc ttcacctatt cgttagatga ctttgccgtg accttctttg taacaggaaa
    32221 tggcttttca accctatcag tcgagattta ctctcgtgct cgcaagggga tttccttaga
    32281 aatcaatgcc ctgtctgctc tagtctttct ctttagtatt atcctagttg taggttatta
    32341 ctttatctct cgtgagaagg aggagcaagc atgaaaaaaa tctattcatt tttagcagga
    32401 attgcagcga ttatccttgt cttgtgggga attgcgactc atttagatag taaaatcaat
    32461 agtcgagata gtcaaaaatt ggttatctat aactggggag actatatcga tcctgaactc
    32521 ttgactcagt ttacagaaga aacaggaatt caagttcagt acgagacttt tgactccaac
    32581 gaagccatgt acactaagat aaagcagggt ggaacgacct acgatattgc cattccaagt
    32641 gaatacatga ttaacaagat gaaggacgaa gacctcttgg ttccgcttga ttattcaaaa
    32701 attgaaggaa tcgaaaatat cggaccagag tttctcaacc agtcctttga cccaggtaat
    32761 aaattctcca tcccttactt ctggggaacc ttaggaattg tctacaacga aaccatggta
    32821 gatgaagcgc ctgagcattg ggatgacctt tggaagctgg agtataagaa ttctatcatg
    32881 ctttttgatg gggcgcgtga ggtgctggga ctaggactca attccctcgg ctacagcctc
    32941 aactccaagg atccgcagca gttggaagag acagtggata agctctacaa actgactcca
    33001 aatatcaagg ctatcgttgc ggacgagatg aagggctaca tgattcagaa taatgttgca
    33061 atcggcgtga ccttctctgg tgaagccagc caaatgttag aaaaaaatga aaatctacgt
    33121 tatgtggtac cgacagaggc cagcaatctt tggtttgaca atatggtcat tcccaaaaca
    33181 gttaaaaacc aagactcagc ctatgccttt atcaacttta tgttgaaacc tgaaaacgct
    33241 ttccaaaatg cggagtatgt cggctattca acaccaaacc taccagcgaa ggaattgctc
    33301 ccagaggaaa caaaggaaga taaggccttc tatccagatg ttgaaaccat gaaacaccta
    33361 gaagtttatg agaaatttga ccataaatgg acagggaaat atagcgacct cttcctacag
    33421 tttaaaatgt atcggaagta ggagataaac gtaagaagac gaatcagtca agctggttcg
    33481 tttttaagga atagaagtca gtttttagaa ccctatcttt ttctattcta gttgcttatt
    33541 tgcttgccta gtagtatact aagcctatat tcttcaaacc acgtcagcgt tgccttgccg
    33601 tactcaagta cagcttgagg ctagcttcct agtttgcttt ttgattttta ttgagtatta
    33661 accttacaga aagcggcaac aaataatgaa aatgtctact tttttcaaaa aaagtttctg
    33721 gccaaccttt atgattgtta accaaactgt tatactatcc catttaaagg atggcttgga
    33781 tcgacaatac ctaacaactg agtccatata ttgggtgata ggcaccttta tatttggaaa
    33841 tattctagta gctgtgttta gtaatatgaa aatttgggat aaaaagaaaa acggcagtaa
    33901 gaaaaaatat atcctgaaaa agtaatcaga gatgtaggga attggtctaa ttcaacggag
    33961 atcatttcca gttgacgcaa gcttaaaaaa acgttataat aagaaagttg agaattgatt
    34021 ttcacttgtc ttagtccaga gaaatggcgg tgctgcgagc catctaagct aggaagtcat
    34081 gctactcaat catacaattg cataagaaca agagaatgac aagttcattg aatgaaggtg
    34141 gtaccgcggt ttttcgccct tcgtgatatg agcttgtctt ttgatttttg gaggtgttga
    34201 tgaaaacatt tcttgtcaaa caaaagtttc gtcttggagg cgaacgcttc gctatcaagg
    34261 atgacagggg agaaattgcc tatcaggtgg agggatcatt ttttaagatt cctaaaacct
    34321 ttaccatcta tgatgcggct ggtgaacaag tcagtgagat tagtaaagaa atcttgacct
    34381 tgcttcctcg ttttgagatt cagcttcggg atggctcgag ttttgtcatt cgtaagaagt
    34441 tgaccttctg gcgagataag tatgagtttg ataatctagg tcttcgtatc gagggcaata
    34501 tctgggattt gaatttcaaa ttgctggatg atcgcgatca gctgattgcg gaaattaaga
    34561 aggaactctt ccatctgacc tctacctata ccgtaacggt tcttgaggac gcttatgcag
    34621 acctagtcat ttccctctgt gtcgcgattg actatgtgga aatgctggaa agccaatcac
    34681 attaaacaag taaataagga gacaatatga aacaactatc tagtgcacaa gtacgccaaa
    34741 tgtggcttga tttctgggcg accaaagctc actcagtaga accatcagta agtttggttc
    34801 ctgtaaatga cccaactctt ttgtggatca actctggggt agcaaccctt aagaaatact
    34861 ttgacgggac cattatccct gaaaatccac gtattaccaa tgcccaaaag gctatccgta
    34921 ccaacgacat cgaaaacgta gggaagactg cgcgtcacca taccatgttt gaaatgctgg
    34981 ggaacttctc tatcggggat tacttccgtg acgaagctat cacttgggct tatgagcttt
    35041 tgacaagccc tgaatggttt gatttccctg ctgaaaaact ttacatgacc tactatccag
    35101 acgataaaga ttcttacaac cgctggattg aagtgggagt ggattcaagt cacttgattc
    35161 caattgagga caacttctgg gaaatcggtg cgggaccttc tggaccagat acagaaatct
    35221 tctttgaccg tggggaagcc tttgacccag aaaatatcgg tcttcgcctg cttgcagaag
    35281 atattgaaaa cgaccgttat attgaaatct ggaacatcgt tttgtcacaa tttaacgcag
    35341 accctgctgt tcctcgtagc gaatacaagg aattgccaca taagaacatt gatacgggcg
    35401 ctggtttgga gcgtttggtg gctgttatcc aaggggctaa gaccaacttt gaaacggacc
    35461 tcttcatgcc gattatccgt gaagtcgaga aattgtctgg taaggtttat gaccaagatg
    35521 gcgacaacat gagcttcaag gttatcgcag accacattcg ttctctttca tttgctatcg
    35581 gtgatggtgc ccttccagga aatgaaggtc gtggttatgt tcttcgtcgt cttctccgtc
    35641 gtgcttctat gcatggtcaa aaattgggta tcaacgagcc tttcctttac aaacttgttc
    35701 caactgttgg aaaaatcatg gaaagctact acccagaagt gcttgagaaa cgtgacttta
    35761 ttgagaaaat cgttaagagc gaagaagaat catttgcccg tacccttcac tcaggtcaac
    35821 actttgccca aggcattgtg gcagacttga aagaaaaagg tcaatctgtc attgctggtc
    35881 aagatgtctt taaactctat gatacatacg gattcccagt tgaattgact gaagaaattg
    35941 ctgaagaagc tggtatgact gtagaccgtg aaggttttga agcagccatg aaagaacagc
    36001 aagaacgcgc gcgtgcgtca gctgtcaagg gtggctcaat gggtatgcaa aatgaaactc
    36061 ttcaaaacat cactgtagaa agtgtcttca actacaatac tagccaattg tcttctaaat
    36121 tggtagctat cgttgctgac aatgcagaag taggagctgt ttcagaagga actgcctctc
    36181 ttatctttgc ggaaacgtca ttttatgctg aaatgggtgg acaggtagct gactacggac
    36241 aaatcttgga tgagtcaggt aagattgtgg ctactgtgac caatgttcag aaagccccaa
    36301 atggtcaagc ccttcataca gttgaagtcc ttgcaccgct tgccttgaac caagaatata
    36361 ccttggcaat tgatagcaat cgccgtcacc gtgtcatgaa aaaccacact gcgactcatt
    36421 tgcttcacgc tgcccttcac aatatccttg gaaaccacgc aacacaggca ggatctctta
    36481 acgaagttga attccttcgc tttgacttta cccacttcca agcagtaact gctgaggaac
    36541 ttcgtgcgat tgaacagcaa gttaacgaga aaatttggga agcacttgaa gtgaagacag
    36601 ttgaaacaga tattgacact gctaaagaaa tgggagctat ggccctcttt ggtgagaaat
    36661 acggcaagga agttcgtgtc gtgactatcg gtgactactc tattgaactt tgtggtggta
    36721 ctcatgttgg caacacttct gagattggtc tcttcaaaat tgtcaaagaa gaaggaatcg
    36781 gttcaggaac tcgccgtatc ttggcagtga ctggtaagga agcctttgaa gcctatcgtg
    36841 aacaagagga tgctcttaaa gctgtcgcag caaccttgaa agcacctcaa gtcaaggaag
    36901 tacctcacaa ggtagaagga cttcaagaac aacttcgtca acttcaaaaa gaaaatgctg
    36961 agttgaaaga aaaagccgca gctgcagccg caggcgatat cttcaaagat gctaaggaag
    37021 tcaacggtca tcgttacatt gctagtcaag tgtctgtatc cgatgccggt gcccttcgta
    37081 cttttgcaga taactggaaa caaaaagact actctgatct tcttgtccta gttgccgcta
    37141 tcggtgacaa agtcaatgtc cttgtagcaa gcaagacaaa agaccttcat gcaggaaacc
    37201 ttgtcaaaga attagcacca atcatcgatg gacgtggtgg tggtaaacca gacatggcca
    37261 tggcaggagg aagcaaccaa gctaagatcc aagaattgtt ggatgcagta gcaggtaaat
    37321 tgtaatcaag taaagatcta tccatttggg taggtctttt tgtgaataca aaaaagccaa
    37381 atccgcttgg acctagcttg gttattatga gctattagat ggtattgact gcccagacac
    37441 ttacggatct agctgagaca gggaattgtc cataaccttc ctcatcaatt gtaacttgac
    37501 cttggtggtt accaagtaaa tctacaaagg tttgattagt ccattcttga ccgacaaaca
    37561 ttgacttgct gttttcttgg tcatttgaga taaggactgc gattggggat tgattttcag
    37621 cacctgaacg tacccaaccg atacagttag catggtcaaa gtagtcattt tgttctccat
    37681 aggccaagtc ttttcggatg gctaggaggc ggtcaaggat ttctttgaaa tcttcttgag
    37741 catactggcc tgaaatccca tagtagtctc cgtaaaagac acatggaagg ccgtcttggc
    37801 gtaacaaaat gagggcatag gctgctggct tgaaccattc ttcaacggta gactcaaggg
    37861 cttgtcctcg ttgggtatcg tggttgtcga caaaagtcac agccttgtca ggcttgagtt
    37921 caaccaggct atctgtaaaa atgccacgaa ggtcatagtt tgcgccagct tgactggctt
    37981 caaagagatt ctggtggaga cgaacatcga caaggtcaaa gtgttcttcc gttttttcga
    38041 gatagtccag attggcttcc ttgtctgggt tccaaaattc accaaaaaca tagaaatcgt
    38101 caccgtattt ttccttcata tcgcggatga agttgcgcat aaagaaagag tcaatatgct
    38161 taacggcatc caaacggaaa ccagctacac cagtcgtttc catgaaccaa tcagcccagt
    38221 catagatgtt ttggatgact tcaggatgtt taaagtctag gtcggcatac atgaggtagt
    38281 cgtagtttcc gttttcgtta tcgaccaatt cctcgttagc ccagcccttg ttgtcccctt
    38341 ggatcagata aatcccagat ttactgcgtt tggcatcgta gtctgtaccg gtgaagtggt
    38401 accaatgcca gtggaagcca ttataggtat cttggcgacc atcgaaggta aaactagtcc
    38461 agccattgat ggtgaagggt tctccaagtt caactgtacg gtctacagga tccacttcaa
    38521 taacctgaaa ggcttccctg tgatcggcag cagccttgtg gttgagaact acatcggcta
    38581 ttggttgaat tccctgcgct ttaagggctt gaatggcttg aagatagtct tctttgaaac
    38641 catacttggt gcggacagtc cctttttggt tgaactctcc taggtcaaat aagtcataga
    38701 ccccataacc tacatctttt tcattggttg ccttaaaagc tggtggcatc cagacgtgac
    38761 tgatccccag atgagctagg tgtggagcat tttcagccag acgcgtccag tgttgaccgt
    38821 cgtggggcag ataccattca aagtattgca tgagtgtttg attttgcatg atgtttcctc
    38881 ttacttgtca atcttgttct ttattctatc ataaagtatc tattagcgca aacgtttgcg
    38941 taagatagaa ggaaactatt ttaactactt aaaataaagt attgattttt ttgtatcaaa
    39001 gtgctagtat agcgatgtat aatacagata aagataggag ttgtcccatg attgaatttc
    39061 aaaatgttag taagttgtat ggtgataaag aggccttgag taatctcaat ttgcagattg
    39121 aaaatggaga gattatgggc ttgattggtc ataatggggc tggaaaatcg accaccataa
    39181 aatccctagt cagtatcatt tcacccagca gtggtcgtat tttggtagat ggtcaggatt
    39241 tatcggaaaa tcgcttggcg atcaaacgaa agattggcta cgtagcagac tcgcctgact
    39301 tatttttacg tttaacggcc aatgaatttt gggaattgat tgcttcatcc tatgatttga
    39361 gcagctctga cttggaagct agtttagcta ggttattgaa cgtttttgat tttgctgaaa
    39421 atcgttatca ggttattgaa accctttctc atggaatgcg tcagaaagtt tttgtcatcg
    39481 gggcactctt gtctgatctt gatatttggg tcttggacga acccttgact ggtttggatc
    39541 cccaggctgc ctttgatttg aagcaaatga tgaaggagca tgcgcaaaaa ggtaagacag
    39601 ttttgttttc aactcatgtc ctagaggtag ctgagcaagt ctgtgatcgg attgctattt
    39661 tgaaaaaggg gcatttgatt tattgtggta aggtagagga cttgagaaga gacaacccag
    39721 accagtcttt ggaaagtatc taccttagcc ttgctggtag aaaagaggag gtttcagatg
    39781 cgtctcaagg tcattaaaaa attagttgat atcaatatcc tctattcatc tcaagaagct
    39841 aatctggcta acctacgaaa gaagcaagct aaaaatactg gaaaaaaagt aaatgtttcc
    39901 gctagagtcc taagttctta cattttttcc agtctcttga tgctcatctt ttttagtact
    39961 atagccattc attttccttt tgaggaaatg cctgtttatt ttagttttgt ggttgctatt
    40021 ttactagtga ttgccttttc aacttcttta actgcatttt acaatgtctt ttatgagagt
    40081 aaggacctgg cctcctatag gccctatgcc tttaaagaat cagagattat aattgctaaa
    40141 ggactgtctg tccttttgcc agctctaact ggaattgtac caatcctagc ttattttcta
    40201 gccctctata ttagtctagc tccttctctt tggctgggtt tgcctttgat gctactgtcc
    40261 ttgaccttat tatttgtctc tgtggctcta gtgatggtag tggcagtgca tttcttggct
    40321 caaactacgg ccttcagaaa gtatcagtct attttttcga atgtgatgat tgggatagga
    40381 gttctcatac ctttaatatt tgtcttcttt ctacagtcga cttttggaag tattgttgac
    40441 aaagttagag acattccatt tctcctttat cctcttcata tcttttacaa aatagcagtg
    40501 gagccttttt cgacagaagc cttagtgggt ctgctcgctt ggataggact aactctcttc
    40561 ctgctttatc tgaccaaaaa gaaggtcctt cctcgttttt atgacgtgat cctgcttaat
    40621 agtgaggaga aggtcaaaaa agaacgtcgc agcaaggaga gaatttcaac cactaaaaag
    40681 ggatttttcc gcatggtttt acgctaccac ctcaccctct tgggacaggg tactggcgtg
    40741 gttacagtgc tttttacaag tgctttcctt ccttatctca tgatgatcgg tctgatttcc
    40801 aaaatccgag attctcagat agttccagac attcatcctc catactggtt acccttgttt
    40861 tttatagctc tctttatagc agttgtcaat aacaatatca ccagcctgca ttcaattgcc
    40921 ttgtccttgg agagggaaaa tgttgatttt ctgaagagtt taccctttga ctttgctcgt
    40981 tatgtgaaag tgaaattttg gattatatat gccgtccagt cctttttacc aattctgact
    41041 ttacttggtc tttctctata tctaggcttg cccatcattt cgatgattta ccttattatg
    41101 gcatggatcc ttgccagtgt catcctttct tgccaccatt actttaagga cgttaaaaat
    41161 ctgtcaatca attggagtag cattacggac ctggtgaatc gttcaaatgg catagtcgcc
    41221 atagttttat tgtttattta tagtgcaatc ctgatggccc ttgtaattgg gagcatattc
    41281 ttggttcagt ctctctccac tatccttgcc atcagcttgg gagtaggagc tcttatcctc
    41341 ctgcttgctc ttgctatttt tggctatcat tattacctgt cacgcatatt ggcagaaata
    41401 gaaaaaagat gagctggttg gtcgcttgct tgataaattc aattcgacaa acgcttttat
    41461 agtttgttag aatgtagttg taaatgatta agattggtaa tcaaaaatat agggaggaat
    41521 gttttgaata agaaatataa catagttcta tttttgttgt ttatagttta tttattggga
    41581 tatttttcaa tttcaaaaac gttaattcct ataatgtgtg tgatccaagt atttttgata
    41641 gaacatatat ttagagttcg aaatagaatg atgcaaatag gtgaaattat aat
//
LOCUS       DAVLYV010000010        41448 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLYV010000010 DAVLYV010000000
VERSION     DAVLYV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 41448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.010"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..154
                     /locus_tag="VYO70_000537"
                     /pseudo
     CDS             1..154
                     /locus_tag="VYO70_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(279..482)
                     /locus_tag="VYO70_000538"
     CDS             complement(279..482)
                     /locus_tag="VYO70_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1712819.1"
                     /translation="MIKPYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(475..1557)
                     /locus_tag="VYO70_000539"
     CDS             complement(475..1557)
                     /locus_tag="VYO70_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1712820.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1683..2465)
                     /locus_tag="VYO70_000540"
     CDS             complement(1683..2465)
                     /locus_tag="VYO70_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1712821.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2465..3808)
                     /locus_tag="VYO70_000541"
     CDS             complement(2465..3808)
                     /locus_tag="VYO70_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1712822.1"
                     /translation="MNLKTTLGLLAGRSSHFILSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3907..5223)
                     /locus_tag="VYO70_000542"
     CDS             complement(3907..5223)
                     /locus_tag="VYO70_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1712823.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVTAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5416..6642
                     /locus_tag="VYO70_000543"
     CDS             5416..6642
                     /locus_tag="VYO70_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1712824.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKALT"
     gene            6841..8415
                     /locus_tag="VYO70_000544"
     CDS             6841..8415
                     /locus_tag="VYO70_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1712825.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            8679..9467
                     /gene="codY"
                     /locus_tag="VYO70_000545"
     CDS             8679..9467
                     /gene="codY"
                     /locus_tag="VYO70_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1712826.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            9467..10042
                     /locus_tag="VYO70_000546"
     CDS             9467..10042
                     /locus_tag="VYO70_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1712827.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            10124..10209
                     /locus_tag="VYO70_000547"
     tRNA            10124..10209
                     /locus_tag="VYO70_000547"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10158..10160,aa:Leu,seq:aag)
     gene            10492..11622
                     /gene="ugpC"
                     /locus_tag="VYO70_000548"
     CDS             10492..11622
                     /gene="ugpC"
                     /locus_tag="VYO70_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1712828.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFITVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(11769..11915)
                     /locus_tag="VYO70_000549"
     CDS             complement(11769..11915)
                     /locus_tag="VYO70_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712829.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            12123..12881
                     /locus_tag="VYO70_000550"
     CDS             12123..12881
                     /locus_tag="VYO70_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1712830.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            12968..13480
                     /locus_tag="VYO70_000551"
     CDS             12968..13480
                     /locus_tag="VYO70_000551"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1712831.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            13662..14606
                     /locus_tag="VYO70_000552"
     CDS             13662..14606
                     /locus_tag="VYO70_000552"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1712832.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            14615..15292
                     /locus_tag="VYO70_000553"
     CDS             14615..15292
                     /locus_tag="VYO70_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1712833.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            15390..16148
                     /locus_tag="VYO70_000554"
     CDS             15390..16148
                     /locus_tag="VYO70_000554"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1712834.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            16186..17691
                     /gene="lytC"
                     /locus_tag="VYO70_000555"
     CDS             16186..17691
                     /gene="lytC"
                     /locus_tag="VYO70_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HEV1712835.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELKRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            18189..18707
                     /locus_tag="VYO70_000556"
     CDS             18189..18707
                     /locus_tag="VYO70_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1712836.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGHLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            18837..19343
                     /locus_tag="VYO70_000557"
     CDS             18837..19343
                     /locus_tag="VYO70_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1712837.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNLEEKIDGVATFHDVQSVLDWYQAQEKNLYILGG
                     KQIFQAFESYLDEVIVTHIHARVEGDTYFPEEFDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            19343..19513
                     /locus_tag="VYO70_000558"
     CDS             19343..19513
                     /locus_tag="VYO70_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712838.1"
                     /translation="MERSLFGLFTAFLCFICLLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            19545..20777
                     /gene="clpX"
                     /locus_tag="VYO70_000559"
     CDS             19545..20777
                     /gene="clpX"
                     /locus_tag="VYO70_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1712839.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRMGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            20786..21373
                     /locus_tag="VYO70_000560"
     CDS             20786..21373
                     /locus_tag="VYO70_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1712840.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            21384..21764
                     /locus_tag="VYO70_000561"
     CDS             21384..21764
                     /locus_tag="VYO70_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1712841.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            21816..22706
                     /gene="rapZ"
                     /locus_tag="VYO70_000562"
     CDS             21816..22706
                     /gene="rapZ"
                     /locus_tag="VYO70_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1712842.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            22703..23680
                     /locus_tag="VYO70_000563"
     CDS             22703..23680
                     /locus_tag="VYO70_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1712843.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            23677..24588
                     /gene="whiA"
                     /locus_tag="VYO70_000564"
     CDS             23677..24588
                     /gene="whiA"
                     /locus_tag="VYO70_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1712844.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(24622..25590)
                     /locus_tag="VYO70_000565"
     CDS             complement(24622..25590)
                     /locus_tag="VYO70_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1712845.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            25726..26583
                     /locus_tag="VYO70_000566"
     CDS             25726..26583
                     /locus_tag="VYO70_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1712846.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            26570..27349
                     /locus_tag="VYO70_000567"
     CDS             26570..27349
                     /locus_tag="VYO70_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1712847.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            27373..28725
                     /locus_tag="VYO70_000568"
     CDS             27373..28725
                     /locus_tag="VYO70_000568"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1712848.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSVIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            28799..29173
                     /locus_tag="VYO70_000569"
     CDS             28799..29173
                     /locus_tag="VYO70_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1712849.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            29175..30023
                     /locus_tag="VYO70_000570"
     CDS             29175..30023
                     /locus_tag="VYO70_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1712850.1"
                     /translation="MKLAVFTDSSAYLSAEILQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            30289..31056
                     /locus_tag="VYO70_000571"
     CDS             30289..31056
                     /locus_tag="VYO70_000571"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1712851.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWIDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            31068..32252
                     /locus_tag="VYO70_000572"
     CDS             31068..32252
                     /locus_tag="VYO70_000572"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1712852.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNLQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            32249..34120
                     /locus_tag="VYO70_000573"
     CDS             32249..34120
                     /locus_tag="VYO70_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1712853.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELERAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQDVSFAYENKPILQD
                     FNLLVQVKDRIGIVGDNGVGKSTLLNLIAGSLESTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(34263..35447)
                     /gene="mntE"
                     /locus_tag="VYO70_000574"
     CDS             complement(34263..35447)
                     /gene="mntE"
                     /locus_tag="VYO70_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1712854.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            35751..38447
                     /locus_tag="VYO70_000575"
     CDS             35751..38447
                     /locus_tag="VYO70_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1712855.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LLEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEDHVLTGAELNELSDEDFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            38460..39251
                     /gene="yghU"
                     /locus_tag="VYO70_000576"
     CDS             38460..39251
                     /gene="yghU"
                     /locus_tag="VYO70_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1712856.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTVGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTEDVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            39367..39777
                     /locus_tag="VYO70_000577"
     CDS             39367..39777
                     /locus_tag="VYO70_000577"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1712857.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANHDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            39789..41384
                     /locus_tag="VYO70_000578"
     CDS             39789..41384
                     /locus_tag="VYO70_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712858.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
BASE COUNT        12139 a         7766 c         9482 g        12061 t
ORIGIN      
        1 attctgaaat cggaaatgtt ttaagtcgtt tcagtcgctt aagcaattgg aacaagctat
       61 tgtagactat attgattact acaacaacaa acgaattaag gtaaaactaa aaggacttag
      121 tcctgtgcag tacagaacta aatccttcgg ataaattaat tgtctaactg tttggggtca
      181 gtacacaata cctgatagta tgcgttttat tgtgggaaga ttttcctcat tttctagtga
      241 aattgaactt tttctaacat cttttgatag atgacttctt aatcaattat catttccatt
      301 gttgtttctt ggggtttcat cccttgtctt tgataaaagc ggagagcacc ctcgttatca
      361 ttccagacac ttagggttag attattgcag ccaatttctt tagcataata gcagaaaaat
      421 gacaataaaa tagcccctag atattttgaa aggaaagcat aggggctata tggtttaatc
      481 aaaataaagc aaatctttgc tggtgcttgt aaagaggtcg aagccatcct tggtaacaac
      541 accgcagtct tcaatacgaa caccgacttt accagggata tagatacctg gttcaacaga
      601 gaagcacatg ccttcttcga tgaccatgtc gtttccttcc atgatagatg ggaattcatg
      661 gacatccata ccgataccat gcccgagacg gtggttgaag tactcaccat aaccagcttt
      721 ttcgatgacc tcacgggcag cgcggtccac ttcatgagca gtcacacctg gcttgataaa
      781 gtcaagagca gcttgttggg cttcaagagt caagttgtaa atatctttct tgaattggtc
      841 tggtttgccg acagcgactg tacgagtcat atctgacgca tagccattga ccagaacacc
      901 caggtcaaag aggagaagag catcattttc aaccttatta gctgctggaa tgccgtgtgg
      961 attcgcagca ttatcaccag tcaagaccat ggtatcaaag ctcatttcgt aaccttcacg
     1021 tttcatggca aagtcaattt gtgcgatgat atctgtctca gtcttatcaa gagaaatatt
     1081 atcaaaacca acatgaacag ccttgtcagc ataaagacct gcaaccatca ttttttgcac
     1141 ttcatcagct gatttgatga ggcgcatgcg ttggatacga ggagtgaggt tgtcaaactc
     1201 agcagtctca aaaactgttt tcaaaccatg gtatttggtc aagatgagat tgtcaaactc
     1261 aacagcgaca cgtttgaagt caagttgtgg aagagcatgt ttgatttttt gccatggatt
     1321 ttcagaatcg acatagccca ctactgggaa ggaaacggtg ctacttgcac gttctacttc
     1381 aagagctggg acaaagagga gaggttcctg atccgctagg acaaagagga acatttggcg
     1441 ttcatgggga tcactgtaaa aaccagtgag gtaattgatt gtgacggggt cagatacgac
     1501 agcgacgtct agtttttctg attcaagata tgttaggatt tgttgtaatt tagacatatg
     1561 ctaccttctt tctacccctc tattttggca aaaaatgaga gaaaatgcaa gggagaaggg
     1621 taaaagaaaa gacaaaaaac tccgacaaga taacggagtt ctttgatatc attttccttt
     1681 gtttaagaaa agtcagcctt gcttttgacg tcactgtact cttcagcgat ttcttggctg
     1741 aggatatcct cataggcagg gagttggata tcctgaccat atttcttctt gagggcagta
     1801 gtaactaggc gataagccag attggcatta cgagagagga taggtccgtg gaagtaggaa
     1861 ccaaagacat tcttataatg aaccccttca ccgacctttt cttcgttgtt tccatttcca
     1921 tagacaacct gtcccagcgg tttttggtca tcagagagga aggtacggcc ttggtgattt
     1981 tcaaatccat agtaggtttc atcgaaatct tcattgtgaa tcttgatgtc accgataaaa
     2041 cggttattgg tctggttgag cgtgtagtgt cccatgaccc ctagcccttc gatacgtttt
     2101 cctgaagctt caacataata ttgacccaat agttggaaac caccgcagat agccagaact
     2161 acaccgtcgt tttggatgta gttgtcaatg ctctcttttt tagcaggtag gtcgtctgca
     2221 atgatacttt gttcaaagtc ttgaccacca ccgaaaaagg cgatgtcgta gtgattttca
     2281 tcaaagtcat catggagaga aacgatgtca acggtcacat gggctcccag tttttcagcc
     2341 acatacttga gcatgaggat gtttccattg tccccgtagg tattcatgag atttccgtag
     2401 aggtgggcaa tgttgagctg ataggggtaa ttgccatctt ttgaggaaag tgaagtataa
     2461 accattagtt catctccttt ctaacaatct gacgactagc cagcagttca cgaaattcca
     2521 gcatggcagt ataagttgcc agaatatagg catgcttgca gtcttgattc tcaatggtct
     2581 tgagaacttg ctccagatta ctcgtttcag tgattttctc agctggatag ccagtcactc
     2641 ggaggcgacg agcgatttca gaatgacgaa caccgccagc gttgatttca ggaatgtcca
     2701 tgtcagtgat ttgttcaaag tctgcatccc agatccagct agtgtcaatt ccatctgcat
     2761 agttggcatt aaggaggaca gataggctaa atggataagg tgctagtttg atcatttcga
     2821 tagcttgggt tgcaccgact ggatttttaa tcaagacaag ggtacattcc ttgtcaccga
     2881 tatgaaaggt ttcttggcgt ccaaagacag cacggctctt gtcaaatccc tgtttgatga
     2941 gttgcgaatc ggcacctagg aaacgggcga tggccacagc agctagggcg ttatagatat
     3001 tatagagccc gccgatttgg ataccgtatt cttggccgtc tatgacaaag cgagagcgat
     3061 tgttggtcaa ctcaaccagt tttgtcaaac gatagtcgag atcaggacgt ttgcatccac
     3121 aaccttcaca gatataggca cccaagtttg cataggtatt atgctcatat ttgaggatgc
     3181 cttggcagtc aggacagaga atcccttcgg tattgtagtg agccagttgg gctggtcctt
     3241 tttccaagtc aaaaccaaaa tactctatag ggtttggaat agttggcttg tagaaaagtg
     3301 gactgtctcc gttaaggaga acagtagcag ttggaacttt ccgaatggca tccaatatca
     3361 tgttataggt agtatagatt tcaccgaaac ggtccatctg gtcacggaag atattagtaa
     3421 tgacaaaaag actaggctgg atatagtcac agatacgaga tagactggct tcgtcaattt
     3481 cgaggacggc aatatttttc ccagtttttg aagatttggc tgttaggaag gttgttgcaa
     3541 tccctgtaat catgttggca ccgcttgggt tggttagaac ttgaccataa acctctttta
     3601 aaatgccgac agtgagggca gttgtcaggg tttttccatt tgttccagtg acaacgacaa
     3661 tctcgtagtt cttagctagg ttttgtaaaa tatctttatc aaattgaagg gcgactttac
     3721 ctgggagcgt acttccacgg ccaagacggc ttaaaatgaa gtgggaagaa cgcccagcaa
     3781 gaaggcccaa agtagttttt aagttcatgt ttctattata tcataaaaga ggagaaagac
     3841 agatttgaga ttccgcagaa gtaaaaacag cccaaagagg gctgttcgat acgattaaat
     3901 ctcaacttaa atcgcaaaca agtcattcaa gttctcagcc aaggttgggt gagtgaagat
     3961 ttgttttgtg aagtaagtgt aaggaatctt gttgtccata gcaacagtga tgatgttgat
     4021 gatttcttga gaaccttctg agaagatgct tgctccaaga atttcttttg tttcagtatt
     4081 gacaacagct ttgaaggcac cgcgaaggtc tccatttacg tgaccacgag gcattgctgt
     4141 aacggggatt tccttaacag cgtatggaag tttcaaatca gctgcttggc tttcagtcaa
     4201 accaacttgt gaaagtgcag gtgtgatgaa catagtattt ggcacattga gacggtcttc
     4261 aagtgtgtag ctgccatctc cagcaaggta gctgtaaaca acacggaagt catcaagtga
     4321 aatgtaagta aattgaaggc caccgttgac atctccaact gcaaagacac caggaacgtt
     4381 tgtttgacag tgtttgtcta ctttaatagc accacgttca gttagttcaa tatctgtatt
     4441 ttcaagttga agtggttcta catttggttt gcgtccagtt gcgtagagaa gggcgtcgaa
     4501 acggtaagtt tcgtcttcag ttacgacaag cacttggtca ccatcgtttt tgatttcagt
     4561 agtatggata ttttgaagca attcaatgcc atcttcttcc atgtattgtt tagcaagagc
     4621 tgcgatggaa ggttctgcac gaggtaggaa tgtatccaag gcatctagga ctgtgacctt
     4681 gcttccaagt ttgttgtaaa ggccggcaaa ttcaagaccg atatttccgc caccaaggat
     4741 tccaagtttt tcaggtaatt tgtccaagct ttggatacct gttgagtcaa agatgttttt
     4801 gcttgtagca agtccaggga ttggcaagac gtttgaaaca gcaccagtgt tgatgacgat
     4861 tgtttcagca gtcagttctt tcttttcatc accagcttgg atttcgatga ctttatttga
     4921 aaggaagtga gcttccgcat caaagatatc tacgcctgta ccagcaacag tcgcatagtt
     4981 tttaccgttg aggcgaccag tgatcgtgtt tttagtagca atgacttctt caaaagacaa
     5041 gtccttttca gcagcaacta gcaaggtttt agttgggata caaccaatgt tgatacaagt
     5101 tccaccgtac atagccttgc tgcgttcaac gagggcaact tttttgccag ctgaagccaa
     5161 tttacctgct agtgttttac cagctttacc aaatccaata acaattaaat cgtatgttaa
     5221 catttatctt cctcctattc gaatttcatt ctagcacaag aaaaaaactt ttgcacaatt
     5281 ctgctacgga aaagacagaa aaagataaaa acgctttcca gacaaaaaac tagtaatttt
     5341 ttgacaagct tgataaacta aaatcctatc tatttttata taagatagaa taaatgttcg
     5401 aaaataggag gttttatgaa atcgaatcgt tatattattg cctttgctgg ggtcatttta
     5461 cacttaatgc tgggttcgac ttatgcttgg agtgtttatc gtaaccctat tattgaaaaa
     5521 acgggatggg atcaggcttc tgttgccttc gcctttagtc tagcaatctt ttgtttgggc
     5581 ttatcggctg catttatggg gcgtttggta gaaaaatttg gtccgaaagt catgggaagt
     5641 ctatctgctt ttctatacgc aggtggaaat atcttaacag gatttgcaat agaccgtcag
     5701 gagctgtggt tgttgtatct cgcttatggc attttaggtg ggcttggttt gggagcaggc
     5761 tatattaccc ctgtgtcgac gattataaaa tggtttcctg ataaacgtgg tctcgcaaca
     5821 ggtttagcga ttatggggtt tggttttgct tctttattga ctagtcccat agcgcaacac
     5881 ctcatcgcag gggtagggct tgtagaaact ttttatattt taggagcaag ttactttatt
     5941 atcatgctcc tagcttcaca attcattaag cgtccaaatg agcaagagct tgcaatttta
     6001 tcttcttcag ggaaagaaaa aacagcctct ttgacgcaag gaatggctgc aaatcaggct
     6061 ctaaaaagca atcggtttta tatgctttgg attattttct ttatcaacat agcttgtggt
     6121 ttaggcttaa tttcagcggc atcgccaatg gcacaggaga tggctggctt gtctacaagt
     6181 catgcagcag taatggtggg tgttttgggg attttcaatg gatttggtcg cttgctctgg
     6241 gcgagtttgt ctgactatat cggtcgccct ctaaccttta gtatattact gcttgtcaat
     6301 cttttctttt ctctctcact ttggctcttt acagattccg ttttatttgt agttgctatg
     6361 tctattttga tgacttgcta tggagctggt ttttctttga ttccagctta tctcagtgat
     6421 atttttggaa ccaaggaatt ggccgctctg catggttata ttttaacagc ttgggcaatg
     6481 gctggtttag cgggacctat tttattagca gagacttata aaatggctca ttcgtacaca
     6541 caaaccttgt tcgtttttct cattttatac agtatcgcct tggctttgtc ttattatcta
     6601 ggtcgttcaa tcaaaaaaga aagtcaaaaa gcgcttacat gattttctgt agaaatgggg
     6661 ctatcttttg ctctggtctt gtgttatact agataggttg caaagaaaac agtacttttc
     6721 ttttgtggaa aagaagcaac acgattttat atctagttat atgaaaatac gtcataaaaa
     6781 gaaaagtata aactaagccc tatagggtgg cttcgcacca cctttagaaa gaagaataac
     6841 gtgaaattta atgaattaaa cttgtctgct gatttgctag cagaaattga aaaagctggt
     6901 tttgtagaag ctagtcctat ccaagaacaa actattcctt tggcccttga aggcaaggat
     6961 gttatcggtc aagctcagac tggtacagga aaaactgcag cctttggctt gcctaccctt
     7021 gaaaaaatcc gtacagaaga agcgactatc caagccttgg tcatcgctcc aactcgtgaa
     7081 ctagctgtcc aaagtcaaga agaactcttc cgctttggtc gtagtaaggg agtcaaagtc
     7141 cgttcagtat atggcggatc aagcattgaa aaacaaatta aggctcttaa atctggtgcc
     7201 catattgtgg tgggaactcc aggtcgcctc ttggacttga ttaaacgcaa ggccttgaaa
     7261 ttacaagaca ttgaaaccct tatccttgac gaagcggatg aaatgcttaa catgggcttc
     7321 cttgaagaca tcgaagccat tatttcccgt gtacctgaga accgtcaaac tttgcttttc
     7381 tcagcaacta tgccagatgc catcaaacgt atcggtgttc agtttatgaa agcccctgaa
     7441 catgtcaaga ttgcggctaa ggaattgaca acagaattgg ttgaccagta ctatatccgt
     7501 gttaaggaac aagaaaaatt tgacaccatg actcgtctca tggatgtggc acaaccagaa
     7561 ctcgctattg tatttggtcg taccaaacgc cgtgtggatg aattgactcg tggtttgaaa
     7621 attcgtggct tccgtgcaga aggaattcat ggcgacctag accaaaacaa acgtcttcgt
     7681 gtccttcgtg actttaaaaa tggcaatctt gatgttttgg ttgcgacaga cgttgcagcg
     7741 cgtggtttgg atatttcagg tgtgacccat gtctacaact acgatattcc acaagatcct
     7801 gagagttatg ttcaccgtat cggtcgtaca ggtcgtgctg gtaagtcagg tcaatctatt
     7861 acttttgttg ctccaaacga aatgggttac cttcaaatca ttgaaaactt gactaagaaa
     7921 cgcatgaaag gtctcaaacc tgcaagtgta gaagaatcct tccaatcaaa aaaacaggta
     7981 gctctcaaga aaatcgaacg tgattttgca gatgaaacca ttcgtgccaa ctttgagaaa
     8041 tttggtaagg atgctcgcaa attggctgct gagtttactc cagaagaatt ggcaatgtat
     8101 atcttgagtc tgacagtcca agacccagat agccttccag aagtggagat tgcacgtgaa
     8161 aaaccactac cgtttaaacc atcaggtaat ggtttcggtg gtaaagctaa gggaggtcgt
     8221 ggaggccgtc gtggggacga ccgtcgagag cgtgatcgcc gtggcaatgg tcgccgtgat
     8281 gagttcaaaa aaggaagtcg tggcaacgat cgttttgata aggaaaaacg ttaccgtaag
     8341 gataataaaa aaccacgcaa tactttaagc gaaaagcaaa caggctttgt tattcgtaac
     8401 aaaggcgata aataagaaaa agcggttcaa aatgaatcgc ttttttatat aaggagaccg
     8461 aatgtgaaaa gacaaataaa attattatat tatcttttat aatgttataa tacacaaaaa
     8521 taggcaactt gtcaatagaa aaggaacaat ttaattaaaa atattctggt ttttaattaa
     8581 attgtaagtc aatgcaagtc aataaggaat tttcagataa ttattgaaaa agagacgttt
     8641 ttatgttata atgatagcga ttaaattcga ggtgaaaaat ggcacattta ttagaaaaaa
     8701 ctagaaaaat tacatcaatt ttgaaacgct cagaggagca gttgcaggat gagcttcctt
     8761 acaacgctat tacgcgtcag ttagcggata ttattcattg caatgcctgc attatcaata
     8821 gtaagggacg tctgcttggc tattttatgc gttataaaac aaatacagat cgcgtagagc
     8881 aattcttcca aactaagatt ttcccagatg actacgttca aggggctaat atgatttacg
     8941 aaacagaagc aaacttacct gttgagcatg atatgagtat tttccctgtt gagagtagag
     9001 atgattttcc agatggcttg acgactattg caccgattca tgtatcgggg attcgccttg
     9061 gttctttgat tatttggcgt aatgataaaa aattcgaaga tgaggacttg gttcttgttg
     9121 agattgccag taccgttgtt gggattcagc ttcttaactt ccaacgtgaa gaagatgaga
     9181 aaaatattcg tcgtcgtact gctgtcacca tggcggtcaa taccctttct tactccgaac
     9241 tccgtgctgt ttcagcaatt ttaggggaat taaatggaaa tgaagggaag ttgactgcgt
     9301 cagtgattgc agatcgtatc ggaatcactc gctctgtgat tgtcaatgct cttcgtaaac
     9361 ttgagtctgc ggggattatt gaaagtcgct cacttggaat gaagggaacc tatcttaagg
     9421 tcttgatttc agatattttt gaagaagtga agaaaagaga ttactaatga caaaggcttt
     9481 aatttcgatt gattatacag aagattttgt tgctgatagt gggaaactaa cagcaggtgc
     9541 tccagctcag gcgatttcgg atgccatcag caaggtaact cgattagctt ttgaacgagg
     9601 agattatatc ttctttacta ttgatgctca tgaagaaaac gattgtttcc atccggaaag
     9661 taagttattt cctcctcata atctgattgg gacgagtgga cggaatttat atggagattt
     9721 ggggatcttt tatcaagagc atggttcaga cagtcgtgtc ttttggatgg ataaacgcca
     9781 ttactcagct ttttcaggga ctgacctaga tattcgtttg agagagcgta gagtgtctac
     9841 cgttatctta acaggtgtct tgacggatat ctgtgtccta catacagcta tagatgccta
     9901 taatctagga tatgacatcg agattgttaa accagctgtt gcttccatct ggcctgaaaa
     9961 tcatcaattt gccctaggtc atttcaaaaa tacactcgga gctaagttag tagatgaaaa
    10021 tctaaatgaa ctttctgagt aacctggttg gtgaaaatga aaaaatttga aaaaaagtgt
    10081 tgacaagcat cccaaaagtt gatatactag taaagtaatc gacgcgggga tggcggaatt
    10141 ggcagacgcg caggactaag gatcctgtga ccgctttagg tcgtgagggt tcaagtccct
    10201 ctctccgcat aggaaaagag ctagctaaag ctagctcttt tgtatttctc taaaaaatta
    10261 gaatccagtc aaatgtagtg gagaatcagc gaaaatatgg gaaaactgat ccttgtttct
    10321 gcttttgtca tagaagaata ataaagatta tcttcttcaa attctccgat attctctaaa
    10381 gttttgtgca agttgcacag aacttgttta tttttttggt catcttgcca tagaaatata
    10441 aagcgttttc atatataata taattatcaa aagacaaaag gagttcacct catggtagaa
    10501 ttgaatctta aaaatattta caaaaaatat ccaaacagcg aacactattc agttgaagat
    10561 ttcaacttga acatcaaaga taaagaattt atcgttttcg taggaccttc aggatgtggt
    10621 aaatcaacta cactccgtat gattgctggt cttgaagaca ttacagaagg tactgcatct
    10681 atcgatggcg tagttgtcaa cgacgtagct ccaaaagacc gtgatatcgc catggtattc
    10741 caaaactacg ctctttaccc acacatgact gtttatgaca acatggcttt cggtttgaaa
    10801 ttgcgtaaat acagcaaaga agacattaac aaacgtgttc aagaagcagc tgaaatactt
    10861 ggattgaaag aattcttgga acgtaaacca gctgaccttt caggtggtca acgtcaacgt
    10921 gttgccatgg ggcgtgcgat tgtccgtgat gcgaaagtat tcttgatgga cgaacctttg
    10981 tcaaacttgg atgccaaact tcgtgtatca atgcgtgctg aaatcgctaa aattcaccgt
    11041 cgtatcggag ctacaactat ctatgtaact cacgaccaaa cagaagcgat gacacttgca
    11101 gaccgtatcg ttattatgtc agctactaag aaccctgctg gtacaggtac tatcggacgt
    11161 gtagaacaaa tcggtactcc tcaagaagtt tacaaaaatc cagttaacaa attcgttgca
    11221 ggattcatcg gaagcccagc tatgaacttc atcaccgtga aattggttgg tagcgaaatt
    11281 gtttctgacg gtttccgttt gaaagtgcca gaaggagcat tgaaagttct tcgtgaaaaa
    11341 ggctacgaag gaaaagaatt gatctttggt atccgtccag aagacgtgaa tgcagaacct
    11401 gctttccttg aaacattccc agactgtgtt gtaaaagcga ctatctctgt atcagaactg
    11461 cttggttcag aatctcacct ttactgccaa gttggtaaag acgagtttgt tgcaaaagtt
    11521 gatgctcgtg actacttgca aacaggtgca acagttgagc ttggatttga cttgaacaaa
    11581 gcacacttct tcgatgtaga aactgaaaaa acaatctact aaaataaata aaattcaaag
    11641 cactacaaga aaagatatct ctttatcaat tgtagtggag agatatcagt taatctaggg
    11701 agagaaacaa aatgcttctc tcctttttgc tagagaagtc atattatgca tctatattgt
    11761 gatgctcttt aatactcttc gaaaatctct tcaaaccacg tcaacgtcgc cttgccgtac
    11821 gtatgattac tgatttcgtc agttttatct gcaacctcaa agatgtactt tgagcagctt
    11881 acggctagtt tcctagtttg ctctttgatt tccattgagt attatttgtg ggtaccatct
    11941 acaagtgaag ctatatgcgt aaactacgtg agcaattgaa ttcgaactag agaggtaata
    12001 ataaatttat gctatagtta tggtgacttg tatgcttttg attctagttt atcaaataat
    12061 agattagaat tgtcagataa tatcattttg tgttataatg aagaaaaaac agaggtgttc
    12121 aaatgtcaga agcaggtcat aagtttttag caaaattggg gaaaaaacgc ttacgtccag
    12181 gtggaaagcg tgccacagat tggttaattg cagaaggagg attttcaaaa gaaaagagaa
    12241 tactagaggt tgcgtgtaat aggggaacta cagcaattga gttggcacag cgttttggtt
    12301 gcaagataac tgctgttgat atggatgctc aagctttaga agtggctaaa aaatctgctg
    12361 gaacggcagg tgttgctcat ttaatcagtt ttgaaagagc aaatgcaatg aaacttcctt
    12421 atcaagatgc tagttttgat attgttataa atgaagctat gctgactatg caagccgatc
    12481 aagctaagaa aaaatgtgta atggaatatc taagggtatt aaaacctgga ggtcttctct
    12541 tgacacatga tgtgcttctt aaggaagcta aagagtctat cagacaggaa ttatcacaag
    12601 caattcatgt aaatgtaggt cctttaactc aagatggttg ggaacaggtg atgatagaat
    12661 caggttattg tgatgtgaaa gcattgactg gtgaaatgac attaatgaaa ttatcgggta
    12721 tgatttatga cgaaggtttg ctaggaactt tgaaaatttg tgtaaatgct tgtaaaaagg
    12781 agaatagaaa gcagttttta actatgtata aaatgtttgc taagaataaa cagaaattgg
    12841 gctttattgc gatggctagt tataaatcgt caaaacgtta gataattatt gaagttaact
    12901 tttccttttt tctttcttaa aaaatatgct ataatagaga gtaaaaaact ttgaaagaaa
    12961 gaaaaagatg aatttaaaag attacattgc aacaattgaa aattatccaa aggaaggcat
    13021 taccttccgt gatattagtc ctttgatggc tgatggaaat gcttatagct acgctgttcg
    13081 tgaaatcgtt cagtatgcta ctgacaagaa agtcgacatg atcgtgggac ctgaagctcg
    13141 tggatttatc gtgggttgtc cagttgcctt tgagttggga attggttttg cgcctgttcg
    13201 taagccaggt aaattgccac gcgaagttat ttctgctgac tatgaaaaag agtacggtgt
    13261 cgataccttg actatgcacg cggatgccat taagccaggt caacgtgttc ttattgtaga
    13321 tgaccttttg gcgacaggtg gaactgttaa ggcaactatc gagatgattg aaaaacttgg
    13381 tggtgttatg gcaggttgtg ccttccttgt tgaattggat gaattgaacg gccgtgaaaa
    13441 aattggtgac tacgactaca aagttcttat gcattattaa tgaaaacagt ccctagggct
    13501 gttttctcta cactaggata taaaaataga ctataactag ttagagaaaa actataattg
    13561 aaaactatat cttcttacag tataataaaa ggactaagtg tttgagattt gtcttcaaac
    13621 atatgcaatt attcctgaaa gagtacagtt aggagagggt tatgccgatt cgaattgata
    13681 aaaaattgcc agctgttgag attttacgga cagagaatat ctttgtcatg gatgatcaac
    13741 gtgctgccca ccaagatatc cgtcctttga agattttaat tttaaatctc atgccacaga
    13801 aaatggtcac agagacccag ttgttgcgcc acttggctaa tacaccccta caactggata
    13861 ttgattttct ctatatggag agccaccgtt ctaaaacaac tcgttcagag cacatggaga
    13921 ccttctataa aacttttcct gaagtcaagg atgagtattt tgatgggatg atcatcacgg
    13981 gtgctccagt tgagcattta ccatttgagg aagtggacta ttgggaggaa tttagacaga
    14041 tgcttgagtg gtctaagact catgtctatt cgacccttca tatctgttgg ggggctcagg
    14101 ctgggcttta tctgcgctat ggtgtagaaa aataccagat ggacagtaag ctatcaggta
    14161 tttatcctca ggacacccta aaagagggtc accttctatt tagaggcttt gatgatagct
    14221 atgtatcccc tcattcacgg cacacggaga tttctaagga agaggtctta aacaagacca
    14281 atctcgagat tttatcagaa ggacctcagg ttggggtttc tattttggcc agtcgtgatt
    14341 tacgagaaat ttatagtttt ggtcatttgg agtatgaccg tgataccttg gcaaaagagt
    14401 attttcgaga tcgtgatgca ggtttcgacc cacatattcc agaaaattac tttaaagatg
    14461 atgatgttaa tcaggtacct tgtctttgtt ggtcttcatc tgcagccctc tttttcagta
    14521 attgggtaaa ccatgcggtc tatcaggaga cgccttttga ttggagaaag atagaagatg
    14581 atgcatctgc atatgggtat ttataagagg aattatgaca tatttagacg cttttaaatc
    14641 aggtaccttg gttttaccga gtgccctgct cttgcatttt aaggaactct ttccttctag
    14701 cgacgatttt ctggtttggc aatttttcta tttgcaaaat acgacaggct tagaagaaat
    14761 gtcgccaagc cagattgctg aaaggattgg caaggaaatt tcggatgtca accagtccat
    14821 ttctaatctg acggaaaggg gactgctcca gtatcgtact atcgaattaa atggcgaaat
    14881 tgaattgctc tttgatgcta gtttggcctt ggaacgtttg gatgacctgt ttggagcagt
    14941 tcattcaagt tcagaccagc taacacctca aaaccagctc aaggatttgg tggaaacctt
    15001 ccagcaggag ttgggacgat tgttgacgcc ttttgagatt gaggatttga ccaagacact
    15061 aaaggaagat ggaaccagtg ctgacttgat taaggaggct cttcgtgaag ctgttttgaa
    15121 tggaaaacca aactggaagt acattcaggc gattttgaga aactggcgcc atgaaggaat
    15181 caagagtgtg gctcaaattg aggccaagag agcagaaaga gaagcaagca atcctcagtt
    15241 gacacaggta tctgcagatt tcagaaatgc catggatctc tggaaggatt aatccatgca
    15301 agtaggcttg aaatccgagt aagatttgca agctgtgtat aattgtgata gaataaatag
    15361 aaaataaatt gaaaaaagag gtatgtgaaa tgtcacgtaa accatttatc gctggtaact
    15421 ggaaaatgaa caaaaatcca gaagaagcta aagcattcgt tgaagcagtt gcatcaaaac
    15481 ttccttcatc agatcttgtt gaagcaggta tcgctgctcc agctcttgat ttgacaactg
    15541 ttcttgctgt tgcaaaaggc tcaaacctta aagttgctgc tcaaaactgc tactttgaaa
    15601 atgcaggtgc tttcactggt gaaactagcc cacaagtttt gaaagaaatc ggtactgact
    15661 acgttgttat cggtcactca gaacgccgtg actacttcca tgaaactgac gaagatatca
    15721 acaaaaaagc aaaagcaatc tttgcgaacg gtatgcttcc aatcatctgt tgtggtgaat
    15781 cacttgaaac ttacgaagct ggtaaagctg ctgaattcgt aggtgctcaa gtatctgctg
    15841 cattggctgg attgactgct gaacaagttg ctgcctcagt tatcgcttat gagccaatct
    15901 gggctatcgg tactggtaaa tcagcttcac aagacgatgc acaaaaaatg tgtaaagttg
    15961 ttcgtgacgt tgtagctgct gactttggtc aagaagtcgc agacaaagtt cgtgttcaat
    16021 acggtggttc tgttaaacct gaaaatgttg cttcatacat ggcttgccca gacgttgacg
    16081 gtgcccttgt aggtggtgcg tcacttgaag ctgaaagctt cttggctttg cttgactttg
    16141 taaaataatc agtaagtagc aaaagctagg tggaacagca ttcagatgtc tgttacattt
    16201 tttataggag agcaaagatt gaaaacaaaa attggattag caagtatctg tttactaggc
    16261 ttggcaacta gtcatgtcgc tgcaaatgaa actgaagtag caaaaacttc gcaggataca
    16321 acgacagctt caagtagttc agagcaaaat cagtcttcta ataaaacgca aacgagcgca
    16381 gaagtacaga ctaatgctgc tgcccactgg gatggggatt attatgtaaa ggatgatggt
    16441 tctaaagctc aaagtgaatg gatttttgac aactactata aggcttggtt ttatattaat
    16501 tcagatggtc gttactcgca gaatgaatgg catggaaatt actacctgaa atcaggtgga
    16561 tatatggccc aaaacgagtg gatctatgac agtaattaca agagttggtt ttatctcaag
    16621 tcagatgggg cttatgctca tcaagaatgg caattgattg gaaataagtg gtactacttc
    16681 aagaagtggg gttacatggc taaaagccaa tggcaaggaa gttatttctt gaatggtcaa
    16741 ggagctatga tgcaaaatga atggctctat gatccagcct attctgctta tttttatcta
    16801 aaatccgatg gaacttatgc taaccaagag tggcaaaaag tgggcggcaa atggtactat
    16861 ttcaagaagt ggggctatat ggctcggaat gagtggcaag gcaactacta tttgactgga
    16921 agtggtgcta tggcgactga cgaagtgatt atggatggtg ctcgctatat ctttgcggcc
    16981 tctggtgagc tcaaagaaaa gaaagatttg aatgtcggct gggttcacag agatggtaaa
    17041 cgctatttct ttaataatag agaagaacaa gtgggaaccg aacatgctaa gaaaatcatt
    17101 gatattagtg agcacaatgg tcgtatcaat gattggaaaa aggttattga tgagaacgag
    17161 gtggatggtg tcattgttcg tctaggttat agcggtaaag aagacaagga attggcgcat
    17221 aacattaagg agttaaaacg tctgggaatt ccttatggtg tctatctcta tacctatgct
    17281 gaaaatgaga ccgatgctga gaatgacgct aaacagacca ttgaacttat aaagaaatac
    17341 aatatgaacc tgtcttaccc tatctattat gatgttgaga actgggaata tgtaaataag
    17401 agcaagagag ctccaagtga tacagacact tgggttaaaa tcatcaacaa gtacatggac
    17461 acgatgaagc aggcgggtta tcaaaatgtg tatgtctata gctatcgtag tttattacag
    17521 acgcgtttaa aacacccaga tattttaaaa catgtaaact gggtagcggc ctatacgaat
    17581 gctttagaat gggaaaaccc ttattattca ggagaaaaag gttggcaata tacctcttct
    17641 gaatacatga aaggaatcca agggcgcgta gatgtcagcg tttggtatta agcgatgatt
    17701 tgaaagaggg atgtgatagt agcaccctct ttttctttgt tttatgatag ttcatcctcg
    17761 agtaaattca agatcttgct cggaaatgaa gcttatatag tagattgaat atagacaaat
    17821 accttgtgat tggtaaaaca ttttagaaat tcatttacct ttcctaatcg acttggtttc
    17881 atcttatttc aatctattat agtattgggg aatttcttca aaccacatca gcttggtcag
    17941 ttctacctgc gacctcaaaa cttgtgcttt ggtcaagctg ggtttagttt cctagtttgc
    18001 tgatggattt ccattgacta taagcatccg accctctttt tgtcttctaa agaattctta
    18061 aattatcagt ctattgcaac ttttctcatg tgggtcattt ggcttgcaat tgtctttcct
    18121 tagtagtata ctgaggtagt aatcattaag aagtggttac ataaaaataa tgaatgaggt
    18181 aaaataaaat ggtagaattg aaaaaagaag cagcaaaaga tgtcgcaaca ttaacaaaag
    18241 cagcgccagt agcattggca aaaacaaagg aagttttgaa tcaagcagtt gctgatttgt
    18301 atgtagctca cgttgctttg caccaagtgc actggtatat gcatggtcgt ggtttccttg
    18361 tatggcatcc aaaaatggat gagtacatgg aagctcttga tggtcattta gatgaaatca
    18421 gcgaacgctt gatcacactt ggtggaagcc cattctctac tttgacagag ttccttcaaa
    18481 acagtgaaat cgaagaagaa gctggtgaat accgtaatgt tgaagaaagc ttggaacgtg
    18541 ttctagctat ttatcgctac ctcatcactc ttttccaaaa agcgttggat gtgactgacg
    18601 aagaaggcga tgatgttaca aacgatattt ttgttggtgc taaggctgaa cttgagaaaa
    18661 cagtttggat gcttgccgca gaacttggac aagcacctgg tttgtaagaa acaagacaac
    18721 atagataaat ctgtaggaaa tttcttacag attttttcta ttttgtaagc tattccaaac
    18781 cagtcttttt ttgagttttt tgataaaata gtactatcag tgaaaaggat ggaagcatga
    18841 ctaagaaaat cgtagctatt tgggcccagg atgaagaggg tgtgattggt aaggacaatc
    18901 gtctgccttg gcatttgcca gcagaattgc agcactttaa agaaacaact ctgaatcatg
    18961 ctatcttgat ggggcgggta acctttgatg gaatggggcg tcgcctgctt ccaaaacggg
    19021 agaccttgat tttgacacgt aacctagaag aaaagataga tggggttgct acttttcacg
    19081 atgtccagtc tgtcctggat tggtatcagg ctcaagaaaa gaatctctac attctcggtg
    19141 gcaagcagat ttttcaggct tttgagtcct atttggatga agtgattgtc actcacattc
    19201 atgctcgggt ggagggagat acctattttc ctgaggagtt tgacttatct ctttttgaga
    19261 cagtttcaag caaattttac gccaaagatg agaagaatcc ttatgatttt accatccaat
    19321 atcgcaagag aaaggaagtc taatggaacg gagtttattt ggtttattca cggcttttct
    19381 gtgtttcatc tgccttctgg caggagcaca ggcttttcgt aaaaagcgtt acgggctttc
    19441 tatcctcctt tggttaaatg cctttaccaa tctggtcaat agtattcatg ctttttacat
    19501 gaccttattt tagatagaat gacagtatag aatggaagga aatcatgtct acaaatagaa
    19561 aaaatgatat gatggtttat tgctcatttt gtggcaaaaa ccaagaagaa gtacaaaaaa
    19621 taatcgctgg caacaatgct tttatttgta atgaatgcgt ggagttggct caggaaatca
    19681 ttcgagaaga attggttgag gaagtcttgg cagacttgtc tgaggtgcca aaaccaattg
    19741 aactcctcca tatcttgaac cactatgtta tcggtcaaga tcgtgccaag cgtgccttgg
    19801 cagtggcggt ttataaccac tacaaacgta tcaatttcca cgatacgcgt gaagagtcag
    19861 aagatgtgga tttgcagaaa tcaaacattt tgatgattgg cccaactggt tcagggaaaa
    19921 ctttccttgc ccagaccttg gctaagagct tgaacgtgcc ttttgcgatt gcggatgcga
    19981 cagctcttac tgaggctggt tatgtaggtg aggacgtgga aaatatcctc cttaaactct
    20041 tgcaggttgc tgactttaac atcgaacgtg cagagcgtgg cattatctat gtggatgaaa
    20101 ttgacaagat tgccaagaag agtgagaatg tgtctatcac acgtgatgtt tctggtgaag
    20161 gggtgcaaca agcccttctc aagattatcg agggaactgt tgctagcgtg ccgcctcaag
    20221 gtggacgcaa acatccacaa caagagatga ttcaagtgga tacaaaaaat atcctcttca
    20281 tcgtgggtgg tgcttttgat ggtattgaag aaattgtcaa acaacgtatg ggtgaaaaag
    20341 tcatcggatt tggtcaaaac aataaggcga ttgacgaaaa cagctcatac atgcaagaaa
    20401 tcatcgctga agacattcaa aaatttggta ttatccctga gttgattgga cgcttgcctg
    20461 tttttgcggc tcttgagcaa ttgaccgttg atgacttggt tcgcatcttg aaagagccaa
    20521 gaaatgcctt ggtgaaacaa taccaaacct tgctttctta tgatgatgtt gagttggaat
    20581 ttgacgacga agcccttcaa gagattgcta ataaagcaat cgaacggaag acaggggcgc
    20641 gtggacttcg ctccatcatc gaagaaacca tgctagatgt catgtttgag gtgccgagtc
    20701 aggaaaatgt gaaattggtt cgcatcacta aagaaactgt cgatggaacg gataaaccga
    20761 ttctagaaac agcctagagg tgactatgga acttaataca cacaatgctg aaatcttgct
    20821 cagtgcagct aataagtccc actatccgca ggatgaactg ccagagattg ccctagcagg
    20881 gcgttcaaat gttggtaaat ccagctttat caacactatg ttgaaccgta agaatctcgc
    20941 ccgtacatca ggaaaacctg gtaaaaccca gctcctgaac ttttttaaca ttgatgacaa
    21001 gatgcgcttt gtggatgtgc ctggttatgg ctatgctcgt gtttctaaaa aggaacgtga
    21061 aaagtggggg tgcatgattg aggagtactt aacgactcgg gaaaatctcc gtgcggttgt
    21121 cagtctagtt gaccttcgtc atgacccgtc agcagatgat gtgcagatgt acgaatttct
    21181 caagtattat gagattccag tcatcattgt ggcgaccaag gcggacaaga ttcctcgtgg
    21241 taaatggaac aagcatgaat cagcaatcaa aaagaaatta aactttgacc caagtgacga
    21301 tttcatcctc ttttcatctg tcagcaaggc agggatggat gaggcttggg atgcaatctt
    21361 agaaaaattg tgaggaaaag aaaatggcaa aaacaattca tacagataag gccccaaagg
    21421 ctatcggacc ctatgttcaa ggaaaaatcg ttggcaacct tttgtttgct agcggtcaag
    21481 ttcccctatc ccctgaaact ggggaaattg taggagagaa tatccaagaa cagacagagc
    21541 aagtcttgaa aaacatcggt gctattttgg cagaagcagg aacagacttt gaccatgttg
    21601 tcaaaacaac ttgcttcttg agcgatatga acgactttgt tccttttaat gaggtttacc
    21661 aaacggcctt caaagaggaa ttcccagctc gttcagctgt ggaggtagct cgtcttcctc
    21721 gtgatgtaaa agtcgaaatt gaagtcatcg cagagattgg ataagctagt tgaagtttgg
    21781 tgttgccaaa cttcttttga tataaggaga aaaagatgac aaagaaacaa cttcacttgg
    21841 tgattgtgac agggatgagt ggcgcaggga aaactgtagc cattcagtcc ttcgaggatc
    21901 taggttattt caccattgat aatatgccgc cagctctctt gcctaagttt ttgcagctgg
    21961 ttgaaattaa ggaagacaat cctaagttgg ccttggtagt ggatatgcgt agccgttctt
    22021 tcttttcaga gattcaagct gttttggatg agttggaaaa tcaagatggt ttggatttca
    22081 aaatcctctt tttggatgcg gctgataagg aattggtcgc tcgttacaag gaaaccagac
    22141 ggagtcaccc actagcagca gacggtcgta ttttagatgg aatcaagttg gaacgtgaac
    22201 tcttggcacc tttgaaaaat atgagccaaa atgtggtgga tacgactgaa ctcactccac
    22261 gtgagctgcg caaaaccctt gcagagcagt tttcagacca agaacaagcc cagtctttcc
    22321 gtatcgaagt catgtctttc ggatttaagt atggaatccc gattgatgcg gacttggtct
    22381 ttgatgtccg tttcttgcca aatccctatt atttaccaga actgagaaac caaacgggtg
    22441 tggatgaacc tgtttatgat tatgtcatga accatcctga gtcagaagac ttttatcaac
    22501 atttattggc cttgattgag ccgattctgc caagttacca aaaggaaggt aagtccgttt
    22561 tgaccattgc catgggatgt acgggtggac aacaccgtag tgtggcattt gctaaacgct
    22621 tggtgcagga cttatccaag aattggtctg ttaatgaagg gcatcgcgac aaagaccgca
    22681 gaaaggaaac ggtaaaccgt tcatgagaaa accaaagata acggtgattg gtggagggac
    22741 tggaattccc gtcattctaa aaagtctgcg ggaaaaagat gtggaaatcg cagctatcgt
    22801 gacggtggca gatgatggtg gttcttcagg tgaactccga aaaaatatgc aacagttgac
    22861 accgccaggt gatcttcgta atgtccttgt ggccatgtcg gatatgccta agttttatga
    22921 gaaggtcttt cagtatcggt tctctgagga tgccggagcc tttgctggcc atccattggg
    22981 aaatctcatc attgctggct tgtcagaaat gcagggttca acctataatg ccatgcagtt
    23041 attgagcaaa tttttccata caacagggaa aatttatcct tccagtgacc atcctttgac
    23101 ccttcatgca gtctttcagg atgggacaga agtggctgga gagagtcata ttgtagacca
    23161 tcgaggcata attgacaatg tctatgtgac caatgcccta aacgatgata cgcctctggc
    23221 cagccgtcga gtagtgcaga ccatccttga aagtgacatg attgtcctag ggccaggttc
    23281 tctctttacc tctattttgc ccaatatcgt gattaaggaa attgggcggg ctcttttgga
    23341 aaccaaggca gaaattgcct atgtctgcaa tatcatgacc caacgtgggg agacggaaca
    23401 ctttacagat agcgaccacg tggaagtctt gcatcgtcac cttggtcgcc cttttatcga
    23461 cactgtcttg gtgaatattg aaaaagtgcc tcaggaatac atgaattcca accgttttga
    23521 tgaatacttg gtgcaagtgg aacacgattt tgtaggtctt tgtaagcaag tttcgcgcgt
    23581 gatttcatct aacttccttc gtctggaaaa tggcggtgcc ttccacgatg gagatttgat
    23641 tgtggacgag ttgatgcgca ttatacaggt gaaaaaatga gtttcacagt agcagtaaaa
    23701 gaagaaatcc tgggccaaca ccatctgagc tggcatgaat tatctgccat tatcaaaatg
    23761 tctggtagca tcggtctctc gacttcgggc ttgactttgt cagttgtgac agaaaatgcc
    23821 aaactggccc gtcacctcta tgagtccttt ctccatttct atgaaatcaa atctgaaatt
    23881 cgtcaccacc aacggagcaa tcttcgcaag aatcgcgtct ataccgtttt tacagatgaa
    23941 aaggtgcagg atttgttaag tgatttgcac ttggcagact ctttctttgg cctggaaaca
    24001 ggtattgatg aggcgatttt atcggatgag gaagcaggtc gtgcctatct ctgtggcgct
    24061 ttcttggcaa atgggagcat tcgtgaccct gagtcaggca agtaccagtt ggaaatcagt
    24121 tctgtttatc tggaccacgc gcaaggaatt gcctcccttc tccaacagtt tttactggat
    24181 gccaaggtgc ttgagcgcaa gaagggggct gtgacctatc tccagcgagc agaagacatt
    24241 atggacttct tgatagtaat cggagccatg caggcacgtg atgattttga gcgggttaag
    24301 attttgcgag aaacccgtaa cgacctcaat cgggctaata atgccgagac agctaatatc
    24361 gctcggacag tttctgccag catgaagacc attaacaata tcagtaaaat aaaagatatc
    24421 atgggcttag aaaatttgcc agtagatttg caggaagtgg cgcaactgag gattcagcac
    24481 ccagactact ctatccaaca gttggcagat agcctcagca cccctctgac caaaagtggt
    24541 gtcaatcaca gactcagaaa aatcaataag atagcggatg aattataaaa ccacgaattc
    24601 tatgtgactc gtggttcttt tttataaact agtagagtgt tttggttgta ctttttgttc
    24661 agggtcaatg tagttgatag cgttgttgac agcagttgga gcttctccga ggcctgtcgc
    24721 aatcagatca atttttccgt catagtagca gcagtcaccg atagcataga tacctgcttg
    24781 gctggattcc tgtttgctgt tgacgataat cttgtgacgg ttgaggtcga gcccccagtt
    24841 tttaaggtta ccgacagaag atttgaaacc atagttgaca aagaggtggt ctaggtcaat
    24901 ggtttcagtt tcatcagatt tgacttttgt gatttcaagt ttatcaagtg tttttccatt
    24961 tccaaggagt tggctagggg cgaatggtgt cttgatggtt acagatgatt cttgcaaggc
    25021 ttgaacactg tgttccaagg cacggaaatt atctctgcgg tgaacaaggg tagttggtgc
    25081 gattttttca aaagccaaag cccaatccac agccgagtct cccccaccaa gaatcgtcac
    25141 tttcttacca gcgtattgct gaatgttaga aacgtggtag tggatatttt catagccctc
    25201 aaccccttca agttccagcg gacgtggttt gaaggcaccg ccacccatag cgatgataac
    25261 tgttttagtc aggtgacttc ctttagaagt tgtgatggca aattcttctt cttgtttgtc
    25321 aatctcaaga accgtttcat tgagatgaat aggggtatca aatccattta gctgttcaat
    25381 caagcggtta gtcaactctt ctccagtcag gtttgggaag cctggtacgt ctaggatttc
    25441 cttttcaggg tagagaatag caggttgtcc acctagctgg ggaagagagt cgatgatttg
    25501 aaccttggct tggcgtaggt gggcataaaa ggctgcaaaa agcccgacag gaccaccacc
    25561 cacaatggta atatcataga gttgagacat agtttctcct ttgttttttc tagtcagttt
    25621 attttatcat atttttgttc aatgtaaaat ggactaggat agggaaaaaa atgccagaaa
    25681 atggtataat aggacgaaag tgttttgata gggaggatat cagggatgaa ttttcaacaa
    25741 ttatccaatc tgcaatattg gaccagtttg tttgcaagtc catggacgat agctatcaat
    25801 ctgattgata ttttgattgt tgcttatatt ttataccatt ttacaaaagc tattgcagga
    25861 accaagatta tgattttggt acgtggagtt ttggtgttta ttttagctca aatccttgca
    25921 aatatgattg gtttgactac gatttcttgg ttaatcaatc aaattattac ttatggggtt
    25981 attgcggcgg ttgttatctt ctctccagag attcggactg gtttggaacg tttgggaaga
    26041 gcgacagatt ttttttccaa tgcccctatt agtgctgagg aacagatgat tcgtgccttt
    26101 gttaagtctg ttgaatacat gagtcctcgt aaaatcgggg ccttggttgc tattcagcgt
    26161 gtacgtacct tgcaggagta tatttcgaca ggaattccct tggatgctaa gatttctgca
    26221 gaacttctca ttaacatttt tattcccaac actcccctac atgatggtgc ggtgattatc
    26281 aaagaagaac gtatcgctgt gacgtctgcc tatctgccct tgacaaaaaa cacagggatt
    26341 tccaaggaat ttgggaccag acaccgggcg gctatcggtt tatcagaagt ctcagatgcc
    26401 ttgacttttg tcgtatcaga ggaaacggga ggaatttcga taacctataa tggaaggttt
    26461 aagcacaacc taacacttga tgaatttgaa acagaattac gtgaaatctt acttccaaaa
    26521 gaggaagtgg gtcttagttt taaagaacga ttgctaggag gatggaaaca tgaaaaaaaa
    26581 tagtttatat atcatatcct cactcttttt tgcttgtgtc ttatttgtct atgctacggc
    26641 gacgaatttt caaaacagta ccagtgctag gcaggtaaaa acggaaacct atactaatac
    26701 agtaacaaat gtccctattg acatacgcta taatagtgat aagtatttta ttagcggttt
    26761 tgcttcagaa gtatcagtgg tcttgactgg ggcaaatcgc ctatcgctag ctagtgaaat
    26821 gcaagaaagt acacgtaaat tcaaggttac tgctgaccta acagatgccg gtgttggaac
    26881 gattgaagtt cctttgagca ttgaagattt acccaatggg ctgaccgctg tggcgactcc
    26941 gcaaaaaatt acagtcaaga ttggtaagaa ggctcagaag gataaggtaa agattgtacc
    27001 agagattgac cctagtcaaa ttgatagtcg ggtacaaatt gaaaatgtca tggtgtcaga
    27061 taaagaagtg tctattacga gtgaccaaga gacattggat agaattgata agattatcgc
    27121 tgtcttgcca actagcgaac gcataacagg taattacagt ggttcagtac ctttgcaggc
    27181 aatcgaccgc aatggtgttg tcttaccggc agttatcact ccgtttgata caataatgaa
    27241 ggtgactaca aaaccagtag caccaagttc aagcacatca aattcaagta caagcagttc
    27301 atcggagaca tcttcgtcaa cgaaagcaac tagttcaaaa acgaattaaa aatagaaaag
    27361 gattttataa caatgggtaa atattttggg actgatggag tccgtggaga agctaaccta
    27421 gaactaacac cagaattagc ctttaaacta ggacgttttg gaggctatgt tcttagtcaa
    27481 catgaaacgg aagcgccgaa agtctttgta ggacgtgaca cacgtatttc aggggaaatg
    27541 ttggaatcgg ccttggtggc aggtctcctt tcagtaggga ttcacgtata caaacttggt
    27601 gtccttgcaa caccagcagt agcttacttg gttgaaactg aaggagcaag tgccggtgtc
    27661 atgatttctg ctagccacaa cccagccctt gataacggaa tcaagttctt tggcggtgat
    27721 ggcttcaaac tagatgatga aaaagaagca gaaattgaag ccttgctaga tgctgaggaa
    27781 gacactcttc ctcgtccaag tgcagaaggc ttaggaattt tggtagatta tccagaaggc
    27841 ttgcgtaagt atgaaggata ccttgtttca actggaactc ctcttgatgg aatgaaggtt
    27901 gccttggata cagctaatgg agcagcttct accagtgccc gtcaaatctt tgcagacctt
    27961 ggtgcccaat tgacggttat cggggaaaca ccagacggtc ttaacatcaa ccttaatgtt
    28021 ggttcaacac atccagaagc ccttcaagaa gtggtcaaag aaagtgggtc agttattggt
    28081 ttggcctttg atggagacag tgaccgcttg attgctgttg atgagaatgg tgacatcgtc
    28141 gatggtgaca agattatgta catcatcgga aaataccttt ctgaaaaagg acaattggct
    28201 caaaatacaa ttgtgacaac tgttatgtct aaccttggtt tccacaaggc cttgaatcgc
    28261 gaaggtatta acaaggcagt tactgcagtt ggtgaccgct acgttgttga agaaatgaga
    28321 aaatcaggtt acaaccttgg tggtgaacag tctggtcacg ttatcttgat ggattacaat
    28381 accacaggtg atggtcaatt atcagcagtt caattgacta aaatcatgaa ggaaactggt
    28441 aagagcttat cagagttggc ggcagaagta acgatttatc cacaaaaatt agttaatatc
    28501 cgagtggaaa acgtcatgaa ggaaaaggcc atggaagtgc cagctatcaa ggccatcatc
    28561 gagaagatgg aagaagaaat ggcggggaac ggccgtatcc ttgttcgtcc aagtggaaca
    28621 gagcccctct tgcgtgttat ggcagaagcg cctacaacag aagaagtgga ctactatgtt
    28681 gatactatca cagatgtagt tcgtgctgaa attgggattg actaaatcct agaaaactag
    28741 atgaaaataa aaaattatgg tacaatagct tataatattg tagccgatgg agaaagacat
    28801 gaatcttaaa cgtgaacaag aatttgttag tcagtatcat tttgatgcac gtaattttga
    28861 atgggaaaat gaaaatggag ctcctgaaac caaggtggat gtgaatttcc aattgctcca
    28921 acatgaccaa gaaaatcaag tgacttcctt gattgtcatc ttgagcttta tgattgtatt
    28981 tgataagttt gtcattagtg gaacaatctc acaagtaaac catatcgatg gccgtattgt
    29041 caatgaacca agtgaattga accaagaaga ggtggaaacc ttggctcgtc catgtttgaa
    29101 catgctcaat cgtttgactt acgaagtgac tgaaattgca ttagacttac caggaattaa
    29161 tttggagttc taatatgaaa ttagctgttt tcacagattc ctctgcttat ctcagtgcag
    29221 agatcttgca aagagaagac ttgtttgtct tggatattcc tgtcaatatt gatggtgagg
    29281 aatatgtcga aggcatcaat ctgtctgctg aggaatttta ccaaaaaatg gctcaggctt
    29341 ctgaattgcc taagaccagt caaccaagta ttgccaagtt agatgaaatc ttaacttcgc
    29401 tcaaagaaca aggttataca catgccttgg ggcttttcct atcttctgga atttcaggtt
    29461 tttaccaaaa tatccagtat atggtcgatg actatgaggg cttaaccatt gctttcccag
    29521 acactttgat tacaagtgct cccctaggca tcatggttga aagcgtcttt aattggcgtg
    29581 atcagggcga tgattttgcc agcattcagg ataagctagc cattcaaatc agccgtacgt
    29641 cagcctttat catggtagat gacttggatc atttggtaaa aggtggacgc ctttcaaatg
    29701 gggctgccat tttgggcaat ttgcttagca ttaagccaat cctttatttt aacgatcaag
    29761 gtgtgattga agtttacgaa aaagttcgta ctgaaaagaa ggccaccaaa cgcttaattg
    29821 aaattatcaa ggaaacaacg gcttcaggtc aataccgggt cattgtcatt catgggaatg
    29881 ctcctgaaaa ggctgaagaa ttgcgtcagc atttgcttga ttttggcttg ggttcggatg
    29941 tttcacttgc tacatttggt agtgtcattg gaacgcacct aggagcagga agtattgctc
    30001 taggttatat tccagtgatt tagttggcac ttctaggcta gttaatctta gaaattgaac
    30061 acggactacc tgcctcttga aaaaagatgc ctgtcttgcc tttcgtggaa agtcagtgcc
    30121 attccctatt tttcaggggc agctaaggtc ctttgtatct tgataattga acacgcctgg
    30181 aaccctgtgt gaaaaagata gttcttccaa gaagtagaca cttcttgatc agaattccta
    30241 ttttcacttt gtgttcttac aggctttgta tcttggacag gagtagagat gagtattcga
    30301 gtaattattg ccggttttaa gggaaagatg ggccaggctg cttgtcagat ggtattgact
    30361 gatccagact tggacttggt agcagttttg gatccttttg agtctgagtc agaatggcag
    30421 ggtattcctg ttttcaagga taaggctgat ttagctggtt ttgaagcgga tgtctggata
    30481 gattttacta ctccagctgt tgcctacgaa aatacacgtt ttgctcttga aaatggcttt
    30541 gctccagtag ttggaacgac tggtttcacg agtgaagaaa ttgcagagct aaaagaattt
    30601 tctcgtgccc aagacttggg tggcctgatt gcccctaact ttgccttggg tgctgtctta
    30661 ctcatgcaat ttgcgacgca ggctgccaaa tatttcccaa atgtggagat tattgagctc
    30721 catcatgaca agaaaaagga tgctccgagt ggaacagcca ttaaaacagc tgagttgatg
    30781 gcagaggttc gagagtcaat tcagcaaggt gcagcagatg aggaagagct gattgctggt
    30841 gctcgtggtg ctgactttga tggtatgcgc atccactcag ttcgtttgcc aggcttggta
    30901 gctcatcagg aagtcatctt tggcaatcag ggagaagggt tgaccctccg tcatgactcc
    30961 tatgatcgca tctccttcat gacaggagtc aatttgggaa ttaaagaagt tgtcaagcgt
    31021 catgagcttg tctatggatt agaacactta ttatgagatt aacgcaaatg ccttctgaat
    31081 ttcagaaggc tttaccagta ttagaaaaaa ttaaagaagc aggctttgag gcttattttg
    31141 ttgggggctc tgttcgagat gcccttctcc atagccctat ccacgatgtg gatattgcga
    31201 cgtcttctta tccagaagag accaagcaga tttttccgcg aacagccgat atcggaatcg
    31261 agcatggaac cgtcttggtc ttagatgggg atgaggagta tgaggtaaca acctttcgga
    31321 cagaggatgt ctatgtggac tatcgcagac ccagtgcggt ttcctttgtg cgctcgctag
    31381 aagaagacct caaacgccgt gatttcacag tcaacgcctt tgccttggat gagacaggag
    31441 aaatcgttga cttgttccat ggtttagaag atttggaaaa gcaagtcttg cgagcagttg
    31501 gagtggctag tgagcgtttc aacgaagatg ctttacggat tatgcgtggt ttccgttttc
    31561 aggctagtct tggttttgca cttgagccag aaacatttaa agctatgaag accttgacgc
    31621 cgcttttgga gaaaatttct gtagagcgta ccttcgtcga gtttgataaa ctcttgctgg
    31681 ctccattttg gagaaggggc ttggcttcca tgattgagag tcaagcttat gactatctcc
    31741 ctgatatggc atctagccag gacaagctca acagactgtt tgatttggag actgatttta
    31801 cttttgaatc ctctgaacaa gcctgggcgg ctttactatg ggctttggag attgaaaatg
    31861 cgcagtcatt tttgaaatct tggaagacct cacgccagtt tgccaagcaa gttcaggatt
    31921 tgctgattat tttggctctg cgtgaaaatg gagaattgag caagcgagat tgttatcgct
    31981 ttgatataga tttgctttta caggctgaaa atcttcgtca agctcaagga aaagaagtca
    32041 acctacaagc catcacagaa aaataccaaa gcttgactat ccatgacaag aaagagattc
    32101 agattaatgg cgatattttg atcaaggaat atggctatca gccaggccca gacttgggag
    32161 agattttaac agagattgag tttgccattg tcgatggaga attggagaat aatcgtgaag
    32221 ccatccatgc ttacctgagg gagaaaaaat gagtgatttt atcgttgaaa aactaagtaa
    32281 atccgttggt gacaagaccg tttttaggga tatttccttt attatccatg acttagacag
    32341 aattggttta atcggtgtca atgggactgg caagaccacc cttttggacg tcctttctgg
    32401 tgtttctgga tttgatgggg atgtcagtcc tttttcagct aaaaatgatt accagattgg
    32461 ttacttgact caggatcctg attttgatga tagaaagaca gttttggata cggttctatc
    32521 tagtgaactc aaggaaatcc agctcattcg tgagtatgaa ttgattatgc tcgactatag
    32581 tgaggacaag caggcgcgtt tggaacgtgt catggcagag atggactctc tccaagcttg
    32641 ggaaatcgag agtcaggtca agaccgttct tagcaaattg ggcattcaag acttatctac
    32701 tcctgttggg gaattgtcag gtggtctgag aagacgggta cagttggcac aagtcttact
    32761 tggcaaccac gaccttttgc ttttggatga gccgaccaac catctggata ttgcgattat
    32821 tgagtggctg accctctttt tgaaaaattc taagaagacc gtccttttta tcactcacga
    32881 tcgttatttc ttagacgctt tgtcaacacg gattttcgag ttggagcgag caggcttgac
    32941 agaatatcag ggcaattacc aggactatgt tcgccttaag gcagaacagg acgagcgcga
    33001 tgcagctcta cttcataaaa aagaacaact ctacaaacaa gaactggcct ggatgcgcag
    33061 acaaccgcag gcacgtgcga ccaagcaaca agctcgtatc aatcgtttcc atgatctgaa
    33121 aaaggaagtt tcaggcagta gtgctgagac agacttgact atgaactttg aaaccagtcg
    33181 gattgggaag aaagtcatcg agtttcagga tgtttccttt gcctatgaaa acaagcccat
    33241 tttgcaagat tttaatctct tggtacaggt caaagatcgt atcggaatcg ttggggacaa
    33301 cggtgttgga aaatcaacct tactcaactt aattgcagga agtcttgagt caactaaagg
    33361 acaagttgtg attggggaaa ctgttcgcat cgcctatttc tctcagcaaa ttgagggctt
    33421 ggatgaaagc aagagagtta tcaattacct gcaggaagtg gcagaggagg tcaagaccag
    33481 tggtggttct acgacttcga ttgcagagtt gctggagcag ttccttttcc cacgttcgac
    33541 gcatggaacc ttgattgaga aattgtcagg gggtgagaaa aaacgtcttt atctcctcaa
    33601 actgcttttg gaaaaaccaa atgttcttct tttagacgag ccaaccaatg acctagatat
    33661 tgcaactttg acagtcttgg agaatttctt gcaaggtttt gcaggtcccg ttttaacagt
    33721 cagtcacgac cgctatttct tggataaggt agcgaccaag attctcgctt ttgaggatgg
    33781 caagattcgt cctttctttg gtcattacac cgactatctt gatgaaaaag cttttgaaac
    33841 agatatggcc aatcaagtgc aaaaggccga aaaggaaaaa gtggtcaagg ttcgagaaga
    33901 caagaaacgc atgacctacc aagaaaagca ggagtgggca agtattgaag gtgatattga
    33961 aaccttggaa aaacgtatcg ctgctattga agaggaaatg caggctaacg gctctgactt
    34021 tggtaagctg gctactctcc aaaaagaatt ggatgagaaa aatgaagcac tccttgaaaa
    34081 atacgaacgc tatgagtatc tcagtgaatt tgatagttaa gactattcga aaatcaaagt
    34141 gcaaactatg tcggcttcac attgccgact caagtacagc ctgtgaccgg cttcctaatt
    34201 tgcactttga tttttattga gtataaaaga atagaaaaat cccgtctcat gaaaggattt
    34261 ctctattctt ttttggtttc ttgatgaaag atattttgcc aggtttcgtg gcgacgccag
    34321 atactggtat ggatgatacc atctaactca tagcagatga gtttggtttt ttgactgatg
    34381 gaagtaattt gaatgtcctt gatagcagaa tttaactctt ttttggtctt ataagcctct
    34441 ttatccatct gctctccatc ttggcgaata tagacaaaat catcagtcaa gagttcttct
    34501 agttggtttc cttggtcaat caattgttca cgcataagca attttttata gacattgtct
    34561 aaaatttcat cctcagggat aggtgctggt tcgatatgga catcggtatc aaagacgcca
    34621 aaacgctcct ccagcataga ctcgacctga tccgcgattt catggctttc aaaaacagac
    34681 aagtcaggat tcatctctag tgtaatatcc aggtagatgt tgctaccgta ggtgcgacct
    34741 ctttgcgatt tgaccttgct gattttggga atttccatga tagccttttg gtagtcctcg
    34801 agcaggcggt cgtcaaagcc atctgaaaga ctaaaggaag actcgatgaa gatatcatag
    34861 gcagtcttca agataaagaa agtgatgatg atagcaacca gtttatccac aatcggataa
    34921 ttgaaactac tagctaggat ggcaatggcg gttccaagtg aggtaacagc gtcagaaaga
    34981 ttgtccttag cagctgcctt cagcgccttg gagttggatt tcttactgag gcgagtattg
    35041 tagagataga ccacaaacat aatcgctgca gaaatgattc ctagagttgc accaagagga
    35101 tcaatgaccg tttcttcccg actgagaatc ttttgaatgg tatctcttag aacatcgaaa
    35161 ccgacataga acatgatgat agaagtgatc aagcttgcca aatcttcaat cttccaatga
    35221 ccaaaacggt ggtcacggtc tgcaggctgg cgcgccatcc gaatcccgat taagagggcc
    35281 acatttccaa tgatgtccga tacgttatta aaaccatcgg ccaccaaact ggatgaatga
    35341 aggagatgac cagctgctaa tttggctgca gacaagatca aataggtcga aatactgata
    35401 atggctccac gctcagctaa cttgagattt gagatagatt gcttcattca acttcctttc
    35461 tagtcacata gcattctacc attatactgg ttttcaagga aaaattcaaa taaggcagtt
    35521 tttactcttt gaaaactccc caaaatttgg tataatggta ctactcaagg gagtagctgg
    35581 cagaaacctg tgatagtgtc gtcattccga attttatgct gaaaagtatg ctttccggcc
    35641 ctatcttaaa cagcgagact tgttatgatt aacaggtctc tttttgtttg tcgtgaaaag
    35701 atatgacaga aagagagtct gttttgcaca caatgtcaag gaggagacac atgtcaaaag
    35761 aacaaaaacg ccaagcgttt tatactcaga gccctgaaga ggtcttgcag gctgtggatg
    35821 cgaccgagca aggtttgtca tcaagtgagg cggaaaagcg ccttgccgaa tttgggcaca
    35881 atgaactcga agaaggcgag aaacgatcaa tcttggtcaa attcatcgag caatttaagg
    35941 atttgatgat tatcatccta gttgcggcag caatcttgtc agtcgtgact tctggtgggg
    36001 aagatatcgc agatgccatt atcatcctag ctgtggttat catcaacgct gcctttggtg
    36061 tttaccaaga aggaaaagct gaagaagcta ttgaagccct caaatccatg tctagtccag
    36121 ttgcccgcgt tcttcgtgat ggacacatgg cagagattga ctctaaagaa ttggtaccag
    36181 gcgatatcgt tgcccttgaa gcaggtgacg tggtaccagc ggacctacgt ttgctagaag
    36241 ccaactctct taaaattgaa gaagcagcct tgacgggtga atctgtacca gtcgaaaaag
    36301 acttgtcagt cgagcttgcg acagatgctg gtattggtga ccgtgtcaac atggccttcc
    36361 aaaactcaaa cgtaacctat ggtcgtggga tgggtgttgt tgtcaataca ggtatgtaca
    36421 ctgaagttgg tcatattgct ggtatgcttc aagatgcgga tgagactgat acaccactca
    36481 aacaaaactt gaacaacctt tctaaggtct tgacctatgc tatcttggtc attgcccttg
    36541 ttacttttgt agtgggtgtc ttcattcaag ggaaaaatcc acttggtgag ttgttgactt
    36601 ctgttgccct tgctgttgca gccattccag aaggacttcc tgctatcgtt accatcgttc
    36661 tttctcttgg tactcaagtt ttggccaaac gacattccat cgttcgtaag ttgccagcag
    36721 ttgaaacact tggttcaact gaaatcatcg cttctgataa gactggtacg ctgactatga
    36781 acaagatgac agtcgaaaaa gtcttctacg atgcggttct acatgactca gctgatgata
    36841 ttgaactagg tcttgaaatg ccactacttc gttcagttgt cttggccaat gatacgaaaa
    36901 tcgatgtgga aggtaacttg attggtgacc caaccgaaac agcctttatc caatatgcct
    36961 tggacaaggg ctatgatgtc aaaggtttct tagagaaata tcctcgtgta gctgaattgc
    37021 catttgactc tgaccgtaag ctcatgtcaa cagttcaccc attgccagat ggtcgtttcc
    37081 ttgtagcagt caagggtgcg ccagaccaac tcttaaaacg ttgtcttctt cgtgataagg
    37141 ctggggatat tgctccgatt gatgagaagg ttacaaatct cattcataca aacaattctg
    37201 aaatggctca tcaagccttg cgtgtccttg caggtgctta taagattatc gatagtattc
    37261 cagaaaatct cacttctgaa gagcttgaaa atgatttaat ttttactggt ttgattggga
    37321 tgattgatcc tgaacgtcct gaagctgctg aggctgttcg tgtggctaag gaagctggaa
    37381 ttcgtccaat tatgattaca ggtgaccacc aagacaccgc agaagctatt gccaaacgtt
    37441 tgggaatcat tgacgcaaac gatacagaag accacgtttt aactggtgct gagctcaacg
    37501 aattatctga tgaagacttt gaaaaagttg ttggtcaata ctctgtttat gcccgtgtgt
    37561 ctccagagca caaggttcgt atcgtcaagg cttggcaaaa acaaggtaag gtcgttgcca
    37621 tgacaggtga cggtgtcaat gatgccccag ctctgaaaac agcagacatc ggtatcggta
    37681 tgggaatcac tggtacagag gtttctaagg gggcttctga tatgattctt gcagatgata
    37741 actttgcgac tattatcgtc gcagtggaag aaggacgtaa ggtcttctca aatattcaaa
    37801 agactattca gtacctactt tctgccaata ctgctgaagt attaaccatc ttcctatcaa
    37861 ccttgtttgg ttgggacgtc ttgcagccag ttcatctttt gtggattaac ttggtaacgg
    37921 ataccttccc agcaattgca ctaggtgttg aacctgcgga acctggtgtc atgaatcata
    37981 aaccacgtgg acgcaaggca agcttcttct caggtggtgt tttgagttct atcatttatc
    38041 aaggtgtact ccaagcagct cttgttatga gtgtttatgg ccttgcgatt gcttacccag
    38101 ttcatgtggg tgacaatcat gctattcatg cagatgccct aacgatggcc tttgcaaccc
    38161 ttggtttgat tcagctcttc catgcctaca atgtcaagtc tgtctaccaa tccatcttga
    38221 cagttggccc attcaagtct aagaccttta actggtccat cttggtatcc tttatccttc
    38281 tcatggcaac aatcgttgta gaaccgcttg aaggaatctt ccacgtaacc aaactagact
    38341 tgtcacaatg gggaattgtt atggctggaa gcttctcaat gattatcatc gtcgaaatcg
    38401 ttaagtttat tcaacgcaaa ctcggttttg acaagaatgc gatttaaggg aggtttcgga
    38461 tgtcagcata tcaattaccg accgtatggc aggatgaagc tagtaatcaa ggagctttta
    38521 cggggctaaa cagaccaaca gtaggtgccc gtttcgaaca aaacttgcca aaaggagaac
    38581 aagcttttca gctttattca ctgggaacac caaatggtgt gaaggttact atcttattgg
    38641 aagaattact agaagctggt tttaaggaag cggcttacga cttgtataag attgctatca
    38701 tggatgggga tcaattcgga tcagactttg tgaagctcaa tccaaattcc aagattccag
    38761 ccttattgga ccagtcaggt actgaagatg taagagtctt tgagtctgct catattcttc
    38821 tttaccttgc tgagaaattt ggagcctttt taccaagtaa tcctgtggaa aaggtagaag
    38881 ttttgaattg gctattctgg caagcaggtg cagcaccttt tctaggtggg ggatttggac
    38941 atttcttcaa ttatgctcct gaaaaattgg aatatcctat taaccgtttt acaatggaag
    39001 tgaaacgcca gttggattta ttggataagg aattggctca gaaaccttat attgcaggca
    39061 atgactatac gattgcagat attgctatct ggtcttggta tggacagtta gttcaaggaa
    39121 atctttacca aggttctgca aaattcttgg atgcctcaag ttatcaaaat ctagtaaaat
    39181 gggcagaaaa aattgccaat cgtccagctg ttaagcgtgg cttggaagta acttatacag
    39241 aaattaaata gcaaaaaagt catcttcgga tggctttttg ctacagttga gggaaagggt
    39301 ttgcagttat cggcttttgt gctataattg ctataatata gtaaaaagca agaggagtgt
    39361 gaggaagtgg aaaagaaaat tgtgaaggat atcttatttt tatctcaagt gtctcagccg
    39421 gcaagtcagg aggaccttta tcttgccaga gatttgcagg atacactctt agcaaatcat
    39481 gatacctgtg ttggtctagc tgccaatatg attggggtgc agaagcgcgt gattatcttt
    39541 aatcttggct tggttcccgt ggtcatgttt aacccagtgc ttctgtcctt tgaaggatcc
    39601 tatgaggcag aagaaggctg tttgtccttg gtaggcgtga gatcaactaa gcgttatgaa
    39661 accataaggg ttgcctatcg tgacagcaag tggcaggaac agaccattac cttgacaggc
    39721 ttcccagctc agatttgcca gcatgagctg gatcacttgg aaggacgaat catttaggag
    39781 gaaagcaaat gaaacgaata gtctttgaac ttatttttat cgcaacgacc tggtatatct
    39841 ttttaccgcc ccttaacctg accagctggg aatttctctt cttcctctgt gggcatttgt
    39901 tagttgtggc aatcttattt ggctttggca aggggataaa ccttgtcaaa acggttcatg
    39961 tgcgccacgg taaggcggaa gctgccttaa atcttgaggg tttcaaaatc aatcggttag
    40021 ggaaaattct gttagcttcg attggaggaa ttcttctctt ggcagctttg gtttccttgg
    40081 taacttccag catgtttcag gctaaaaatt atgccaatgt agtcacggtt acggaaaaag
    40141 actttactga atttcctagg agtgacacca gtaaggttcc tatcctagat agaagtactg
    40201 ctgaaaaaat tggagaccgc tacttgggtt ccctaaccga taaggtgtcg caatacgtag
    40261 cggcagatac ctatacccaa ttgacaattg atgggaaacc ttatcgggtc acaccactag
    40321 aatatgcaga ccctatcaaa tggtttaaca atcaagccaa gggaatcggt gagtatatca
    40381 aggtggacat ggtaactgga aatgcggatt tggtggactt gaagacacca atcaagtatt
    40441 cagactcgga gtattttaac cgtgatgtca aacgtcacct gcgcttgaag tacccgacca
    40501 aaatctttaa aactccatct tttgaggtgg acgatgaggg caatcctttc tatgtagcaa
    40561 cggtttacca aaagcaattt ggacttgctg ttcctcgtcc tgcttcagtc attatcttgg
    40621 atgctacaaa tggagaaacc aaggaataca gcttatcaga tgttccagaa tgggtggaca
    40681 ggatctatcc agcagaggaa accattgagc aaatcaacta caacggcaag tacaaggacg
    40741 gtttcttgaa tgccatgatt tccaagaaaa acgtgaccca gactaccaag ggctataatt
    40801 acttgtctat cggtaatgac atctatctct acacaggtgt gacgtcggct aatgcggatg
    40861 agagtaatct tggtttcatc cttgaaaata tgcgaacagg agaaatcact aagtatagct
    40921 tggcttctgc gacagaagaa tcagcccgtg aatcagcaga aggtgctgtt caggagaaat
    40981 cctacaaagc aaccttccca atcctcatca acctcaatga caagcctctc tacatcatgg
    41041 gcttgaagga caatgctggc ttggtcaaag agtacgccct ggtagacgca gtcgagtacc
    41101 aaaatgttat cgttgctact acagtggaag agatgctcag caagtatgcc aataaaaacg
    41161 accttgaaat tgacaatgca acgacagaaa gcatcaatgg agtagtagca gacctcaaat
    41221 cagctgttat caagggagac actgtctact tctttaaagt tgatggcaag atatacaagg
    41281 tcaaggcttc agtatccgat gaccttcctt accttgaaaa tggtaaaacc ttcgaaggtc
    41341 aagtaggaaa agacaattat ctcaagacct ttaaagtaca ataatactct tcgaaaatct
    41401 ctagctctat ctgcaacctc aaaacagtgt tttgagcagc ctgcggct
//
LOCUS       DAVLYV010000011        40026 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLYV010000011 DAVLYV010000000
VERSION     DAVLYV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 40026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.011"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            9..89
                     /locus_tag="VYO70_000579"
     tRNA            9..89
                     /locus_tag="VYO70_000579"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="VYO70_000580"
     tRNA            94..164
                     /locus_tag="VYO70_000580"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="VYO70_000581"
     tRNA            179..251
                     /locus_tag="VYO70_000581"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            262..333
                     /locus_tag="VYO70_000582"
     tRNA            262..333
                     /locus_tag="VYO70_000582"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:294..296,aa:Gln,seq:ttg)
     gene            343..426
                     /locus_tag="VYO70_000583"
     tRNA            343..426
                     /locus_tag="VYO70_000583"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Leu,seq:caa)
     gene            533..1810
                     /locus_tag="VYO70_000584"
     CDS             533..1810
                     /locus_tag="VYO70_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1712859.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VEERFSIYMEAIQKKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIISPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1886..3370
                     /locus_tag="VYO70_000585"
     CDS             1886..3370
                     /locus_tag="VYO70_000585"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1712860.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTTELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVCELDS
                     YIEDPHTAVASAVYKKYQSATGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            3431..4711
                     /locus_tag="VYO70_000586"
     CDS             3431..4711
                     /locus_tag="VYO70_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1712861.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTIVSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4715..5335
                     /locus_tag="VYO70_000587"
     CDS             4715..5335
                     /locus_tag="VYO70_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1712862.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5501..6643
                     /locus_tag="VYO70_000588"
     CDS             5501..6643
                     /locus_tag="VYO70_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1712863.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDVQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAKKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKVISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVAERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7052..7390
                     /locus_tag="VYO70_000589"
     CDS             7052..7390
                     /locus_tag="VYO70_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1712864.1"
                     /translation="MTRYQILLFLLEHSPCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVETAKYAKEQLVVNPPLQHIKVKEEMESILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            7401..7769
                     /locus_tag="VYO70_000590"
     CDS             7401..7769
                     /locus_tag="VYO70_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1712865.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLLYVIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            7876..8494
                     /locus_tag="VYO70_000591"
                     /pseudo
     CDS             7876..8494
                     /locus_tag="VYO70_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(8521..9378)
                     /locus_tag="VYO70_000592"
     CDS             complement(8521..9378)
                     /locus_tag="VYO70_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1712866.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9508..10650
                     /gene="tgt"
                     /locus_tag="VYO70_000593"
     CDS             9508..10650
                     /gene="tgt"
                     /locus_tag="VYO70_000593"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1712867.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLSSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            10751..12568
                     /locus_tag="VYO70_000594"
     CDS             10751..12568
                     /locus_tag="VYO70_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1712868.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPIVQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12721..13872
                     /gene="nagA"
                     /locus_tag="VYO70_000595"
     CDS             12721..13872
                     /gene="nagA"
                     /locus_tag="VYO70_000595"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1712869.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14035..15093
                     /locus_tag="VYO70_000596"
     CDS             14035..15093
                     /locus_tag="VYO70_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1712870.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15244..15609
                     /locus_tag="VYO70_000597"
     CDS             15244..15609
                     /locus_tag="VYO70_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1712871.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15685..16626
                     /locus_tag="VYO70_000598"
     CDS             15685..16626
                     /locus_tag="VYO70_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1712872.1"
                     /translation="MVQEIAQEIIRSAREKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYGNLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16574..17590
                     /locus_tag="VYO70_000599"
     CDS             16574..17590
                     /locus_tag="VYO70_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1712873.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMKSLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17592..17915
                     /gene="comGC"
                     /locus_tag="VYO70_000600"
     CDS             17592..17915
                     /gene="comGC"
                     /locus_tag="VYO70_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HEV1712874.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQDDGRITEEQAKAYKEYHDKQNTS
                     QIVKD"
     gene            17833..18312
                     /locus_tag="VYO70_000601"
     CDS             17833..18312
                     /locus_tag="VYO70_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1712875.1"
                     /translation="MDASRKNRLKPIKNTMINKILVKSLKIKAFTMLESLLVLGLVSI
                     LALGLSGSVQSTFAAVEEQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQK
                     LPVPKGIQAPSGQSITFDRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18317..18577
                     /locus_tag="VYO70_000602"
     CDS             18317..18577
                     /locus_tag="VYO70_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712876.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            18540..19001
                     /locus_tag="VYO70_000603"
     CDS             18540..19001
                     /locus_tag="VYO70_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1712877.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            18979..19425
                     /locus_tag="VYO70_000604"
     CDS             18979..19425
                     /locus_tag="VYO70_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1712878.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKVEQESGEQVFNLGQVSYQNKKTGLVTRVRTDKSQYEFLFSSVK
                     IKEEKTDKKEEVVTDSSEKAEKKNQKRSLKRKRIHSQFNYNALNPE"
     gene            19528..20120
                     /locus_tag="VYO70_000605"
                     /pseudo
     CDS             19528..20120
                     /locus_tag="VYO70_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            20181..21134
                     /locus_tag="VYO70_000606"
     CDS             20181..21134
                     /locus_tag="VYO70_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1712879.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKNEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21185..22375
                     /locus_tag="VYO70_000607"
     CDS             21185..22375
                     /locus_tag="VYO70_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1712880.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22524..22895
                     /gene="rnpA"
                     /locus_tag="VYO70_000608"
     CDS             22524..22895
                     /gene="rnpA"
                     /locus_tag="VYO70_000608"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1712881.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22870..23694
                     /locus_tag="VYO70_000609"
     CDS             22870..23694
                     /locus_tag="VYO70_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1712882.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23713..24699
                     /locus_tag="VYO70_000610"
     CDS             23713..24699
                     /locus_tag="VYO70_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1712883.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24750..25373
                     /locus_tag="VYO70_000611"
     CDS             24750..25373
                     /locus_tag="VYO70_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1712884.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKILDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25504..26961
                     /locus_tag="VYO70_000612"
     CDS             25504..26961
                     /locus_tag="VYO70_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1712885.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            26984..27262
                     /locus_tag="VYO70_000613"
     CDS             26984..27262
                     /locus_tag="VYO70_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1712886.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQAL"
     gene            27342..27827
                     /locus_tag="VYO70_000614"
     CDS             27342..27827
                     /locus_tag="VYO70_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1712887.1"
                     /translation="MNLKQALIDNDSIRLGLEANDWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27844..28509
                     /locus_tag="VYO70_000615"
     CDS             27844..28509
                     /locus_tag="VYO70_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1712888.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRRITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28513..29376
                     /locus_tag="VYO70_000616"
     CDS             28513..29376
                     /locus_tag="VYO70_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1712889.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAVQEAQAF
                     LYPLIKKAGLM"
     gene            29378..30082
                     /locus_tag="VYO70_000617"
     CDS             29378..30082
                     /locus_tag="VYO70_000617"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1712890.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30261..31871
                     /locus_tag="VYO70_000618"
     CDS             30261..31871
                     /locus_tag="VYO70_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1712891.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKSLLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKF"
     gene            32046..33137
                     /gene="ulaG"
                     /locus_tag="VYO70_000619"
     CDS             32046..33137
                     /gene="ulaG"
                     /locus_tag="VYO70_000619"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1712892.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33251..35227
                     /gene="tkt"
                     /locus_tag="VYO70_000620"
     CDS             33251..35227
                     /gene="tkt"
                     /locus_tag="VYO70_000620"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1712893.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35344..35643
                     /gene="yajC"
                     /locus_tag="VYO70_000621"
     CDS             35344..35643
                     /gene="yajC"
                     /locus_tag="VYO70_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1712894.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35690..36118
                     /locus_tag="VYO70_000622"
     CDS             35690..36118
                     /locus_tag="VYO70_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1712895.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36120..36530
                     /locus_tag="VYO70_000623"
     CDS             36120..36530
                     /locus_tag="VYO70_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712896.1"
                     /translation="MENLTNFYEKYRVYLTRPRLEILAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36830..39481
                     /gene="adhE"
                     /locus_tag="VYO70_000624"
                     /gene_synonym="adhC"
     CDS             36830..39481
                     /gene="adhE"
                     /locus_tag="VYO70_000624"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1712897.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12194 a         7144 c         8975 g        11713 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttggggtata gccaagcggt aaggcaaggg actttgactc
      301 cctcatgcgt tggttcgaat ccagctaccc cagttactat ttgccggcgt ggcggaattg
      361 gcagacgcgc tggactcaaa atccagtgtc cgcaaggacg tgccggttcg accccggccg
      421 ccggtatagt atagtgttag gaacgttgtt attcttcgtt ccttttttat attatttttg
      481 gtataattat agttattcaa attttattta gattaagaaa gtgtagggta gtatgtcttg
      541 ttctatcgat ttattaaaac atcggtattt gaaaaatatt aaagaaaatc ctgaattgtt
      601 tgtcggaatt gagttggagt atcctgttgc aagtttagaa ggggatgcta cagatgttga
      661 agttataaag gatctatttc attatttagt ttctactttg gatctcaccg tagcaaaggt
      721 agatgatttt ggcaatttga tccagttagt agatccgata agtcaggatg ctattttatt
      781 tgaagtttcc tatacaacga ttgagtttgc atttggtaag gctgaaacga tacaagaagt
      841 tgaagaacgt tttagtattt atatggaagc aatccagaag aagttagctg aatcaaatca
      901 tgctattgtt ggctgtggta tccatcccaa ctgggataaa aatgagaatt gtccagtggc
      961 ttatccacgc tatcagatgt tgatggatta tttgaatttg agtagaaata ttattaaatc
     1021 agatttacat catttccctg aatatggtac ttttatctgt gggagccagg ttcagctgga
     1081 tatttcaaaa accaactact tacgggtgat taatgctttt actcaaattg aagcggctaa
     1141 ggcttattta tttgcaaact ctgagttttc gggtgcggat tgggatacga aaatttcaag
     1201 ggatattttc tgggaagaat ctatgcatgg tatctatcca gagaatgttg gggtcaatgc
     1261 tagactcctt aatgatgaag ctgatttttt tgactatcta aatcattctg cgatttttac
     1321 tgcggaacgt gatgggcaga cctattattt ttatcctatt caggctgggg actatttggc
     1381 tacgcctgaa atccaagcat ttgctctgaa tggggatgag gttattattt ccccccaaga
     1441 gaaggatttt gaaactcatc gtagttacca gtaccaagac ttaacgactc gaggaacagt
     1501 tgagtttcgt agtgtgtgta cacagccact tgataggact tttgcttctg cagcttttca
     1561 cttgggatta ttggttaatt tagacaagtt agaagcttac ttagaaacag cacctttctt
     1621 taaagtattt ggttatgatt acaagtcttt aaggagacaa ttttctaaga aaaatcttac
     1681 agatgaggaa gaaactacga ttattgaatt ttccaaagac ttactcctac tagctgagga
     1741 gggactagtg gtgagaaata aggaagaaat gacctattta cagcctttga gagaagaatt
     1801 gagcctataa tttctcttat aaagggagaa ttttctgaaa aatcatgata taatggacga
     1861 gactatagat aaaggataga gagtaatgac attagtttat caatcaacgc gtgatgccaa
     1921 caatacagta actgccagcc aagcaatttt gcaaggtttg gcgacggacg gcggtttgtt
     1981 tacaccggtt acttatccaa aggtagattt gaactttgac aaattgaaag atgcttctta
     2041 ccaggaagtt gctaagctag ttttgtcagc atttttagat gactttacag ctgaggagtt
     2101 ggactactgt atcaacaatg cctacgatag caaatttgat actccagcta ttgcaccatt
     2161 agtgaaatta gatgggcaat acaatttgga acttttccat ggttcaacga ttgcctttaa
     2221 ggatatggcc ttgtctattt tgccatactt tatgacgact gctgctaaga aacatggttt
     2281 ggagaacaag attgttatct tgacagcgac atctggtgac acggggaaag ctgctatggc
     2341 ggggtttgcg gatgtgcctg gtactgagat tatcgtcttt tatccaaagg gtggtgtcag
     2401 caaggtacaa gagttgcaaa tgaccactca gaatggtgac aatactcatg ttatcgctat
     2461 agatggtaac tttgacgatg cacaaaccaa cgtgaagcac atgtttaacg acgtggctct
     2521 tcgtgaaaga ttagcggcca acaagttgca attttcatct gctaactcta tgaatatcgg
     2581 tcgtttggtg ccacagattg tttattatgt ttatgcctat gctcagttgg tcaagactgg
     2641 tgagatcgtg gctggtgata agattaactt cacagtacca acaggaaact ttggaaatat
     2701 cttggctgcc ttttatgcca aacaaatcgg tctcccagtt ggtaaattaa tctgtgcttc
     2761 aaatgacaac aatgttttga cagacttctt taaaacacgt gtctatgaca aaaaacgtga
     2821 gtttaaggta acaaccagcc catctatgga tatcttggta tcttcaaact tggagcgctt
     2881 gattttccat cttttgggaa ataatgctga aaagacaact gaacttatga atgccttgaa
     2941 cacgcaagga caatataagt tgacagactt tgatgcagag attttggacc tctttgcagc
     3001 tgaatatgcg actgaggaag aaacggcagc agagatcaag cgtgtttgtg agttagattc
     3061 ttatatcgag gaccctcata cagctgttgc ttcagcagtt tataaaaaat accaatcggc
     3121 cactggagat gtaactaaga cagtgattgc ttcaacagct agtccataca agttcccagt
     3181 agttgcagta gaagctgtaa ctggaaaagc aggtttaaca gactttgaag ccttggctca
     3241 attacatgaa atctcaggcg ttgcagtgcc accagcagtt gatgggcttg aaatagctcc
     3301 aattcgtcac aagacaacag tggcagctgc tgacatgcaa gcagcggttg aggcttattt
     3361 aggactttaa gacagaggga gcaaactcgg ttgggaaacc aactgagttt cttttcatca
     3421 ggaggagaga ttgtttaaga aaaataaaga cattcttaat attgcattgc cagctatggg
     3481 tgaaaacttt ttgcagatgc taatgggaat ggtggacagt tatttggttg ctcatttagg
     3541 attgatagct atttcagggg tttcagtagc tggtaatatt atcaccattt atcaggcgat
     3601 tttcatcgct ctgggagctg ctatttccag tgttatttca aaaagcatag ggcagaaaga
     3661 ccagtcgaag ttggcctatc atgtgactga ggcgttgaag attaccttac tattaagttt
     3721 ccttttagga tttttgtcca tcttcgctgg gaaagagatg ataggacttt tggggacgga
     3781 gagggatgta gctgagagtg gtggactgta tctatctttg gtaggcggat cgattgttct
     3841 cttaggttta atgactagtc taggagcctt gattcgtgca acgcataatc cacgtctgcc
     3901 tctctatgtt agttttttat ccaatgcctt gaatattctt ttttcaagtc tagctatttt
     3961 tgttctggat atggggatag ctggtgttgc ttgggggaca attgtgtctc gtttggttgg
     4021 tcttgtgatt ttgtggtcac aattaaaact gccttatggg aagccaactt ttggtttaga
     4081 taaggaactg ttgaccttgg ctttaccagc agctggagag cgacttatga tgagggctgg
     4141 agatgtagtg atcattgcct tggtcgtttc ttttgggacg gaggcagttg ctgggaatgc
     4201 aatcggagaa gtcttgaccc agtttaacta tatgcctgcc tttggcgtcg ctacggcaac
     4261 ggtcatgctg ttggcccgag cagttggaga ggatgattgg aaaagagttg ctagtttgag
     4321 taaacaaacc ttttggcttt ctctgttcct catgttgccc ctgtccttta gtatatatgt
     4381 cttgggtgta ccattaactc atctctatac gactgattct ctagcggtgg aggctagtgt
     4441 tctagtgaca ctgttttcac tacttgggac ccctatgacg acaggaacag tcatctatac
     4501 ggcagtctgg cagggattag gaaatgcacg cctccctttt tatgcgacaa gtataggaat
     4561 gtggtgtatc cgcattggga caggatatct gatggggatt gtgcttggtt ggggcttgcc
     4621 tggtatttgg gcagggtctc tcttggataa tggttttcgc tggttatttc tacgctatcg
     4681 ttaccagcgc tatatgagct tgaaaggata ggaaatgcaa aaaacagctt ttatttggga
     4741 tttagacggg actttattgg actcttacga agcgatttta tcagggattg aggagacttt
     4801 tgctcagttt tctattcctt atgataagga gaaggtgaga gagtttatct tcaagtattc
     4861 ggtgcaagat ttgcttgtgc gggtggcaga agatagaaat ctggatgttg aggtgctaaa
     4921 tcaggtgcgt gcccagagtc tggctgagaa gaatgctcag gtagttttga tgccaggtgc
     4981 gcgtgaggtg ctagcttggg cagacgaatc aggaattcag cagtttatat atactcataa
     5041 ggggaacaac gcttttacca ttctcaagga cttgggggtg gaatcctatt ttacagagat
     5101 tttaaccagt cagagtggct ttgtgcggaa gccaagtcca gaagcggcta cctatctgct
     5161 agataagtat cagttgaatt ctgataatac ttattatata ggggatcgga ctctggatgt
     5221 ggaatttgcc cagaatagtg ggattcaaag tatcaacttt ttagagtcta cttatgaagg
     5281 gaatcacagg attcaagcgt tagcagatat ttcccgtatt tttgagacta agtgataaaa
     5341 agattgtgtc agttttgtga cagagaccta acaaactatt tcaagtaacc tagtttgtta
     5401 caaggaatag acagttctgt taaataggcc cgagagggct ttttttctac attttttgtg
     5461 ttatgataga caggtactca tttgaaagga atttgaaaga atgaagaaaa gaatgttatt
     5521 agcgtcaaca gtagccttgt catttgcccc agtattggca actcaagcag aagaagttct
     5581 ttggactgca cgtagtgttg agcaaatcca aaacgatttg actaaaacgg acaacaaaac
     5641 aagttatacc gtacagtatg gtgatacttt gagcaccatt gcagaagcct tgggtgtaga
     5701 tgtcacagtg cttgcgaatc tgaacaaaat cactaatatg gacttgattt tcccagaaac
     5761 tgttttgaca acgactgtca atgaagcaga agaagtaaca gaagttgaaa tccaaacacc
     5821 tcaagcagac tctagtgaag aagtgacaac tgcgacagca gatttgacca ctaatcaagt
     5881 gaccgttgat gttcaaactg ttcaggttgc agacctttct caaccgattg cagaagctcc
     5941 aaaagaagta gcatcaagtt cagaagttac aaagacagtg attgcttctg aagaagtggc
     6001 accatctacg ggcacttctg tcccagagga gcaaacggcc gaaacaactc gcccagttga
     6061 agaagcaact cctcaggaaa cgactccagc taagaagcag gaaacacaag caagccctca
     6121 agctgcatca gcagtggaag caactacaac aagttcagaa gcaaaagaag tagcatcatc
     6181 aaatggagct acagcagcag tttctactta tcaaccagaa gaaacgaaag taatttcaac
     6241 aacttacgag gcaccagctg cgcccgatta tgctggactt gcagtagcaa aatctgaaaa
     6301 tgcaggtctt caaccacaaa cagctgcctt taaagaagaa attgctaact tgtttggcat
     6361 tacatccttt agtggttatc gtccaggaga cagtggagat cacggaaaag gtttggctat
     6421 cgactttatg gtagcagaac gttcagaatt aggggataag attgcggaat atgctattca
     6481 aaacatggcc agccgtggca ttagttacat catctggaaa caacgtttct atgctccatt
     6541 cgatagcaaa tatgggccag ctaacacttg gaacccaatg ccagaccgtg gtagtgtgac
     6601 agaaaatcac tatgatcacg ttcacgtttc aatgaatgga taaacccgac ttgataacat
     6661 cattttgacg aatgagatct agctttcgtg atggaaagcg attctcgttc gttttttctt
     6721 tgtcatactc ttcgaaaatc tcttcaaacc acgtcagttt tatatgaaac ctcaaagctg
     6781 tgctttgagc aacctgcgac tagcttccta gtttgctctt tgattttcat tgagtatcaa
     6841 tttgaatgga aaatgggaag ttaccatatt gtaatgagtt aagtaacatt cttgcaatct
     6901 attttacttt gcattatacg tgattagtca aatattgata aatcaataaa aagagagggg
     6961 aagaaatgct agagattcaa gatttactgt atcaactccg cttgtctgag caagcgagta
     7021 cgcaattgtt tgaaaaaagg cttggattag tttgacacgg tatcagattt tactgttttt
     7081 gctggagcat tctccttgta accaaatggc ggttcaggag cgtttgaaaa ttgatcaggc
     7141 tgctttgaca cggcatttca agattttgga aacggaaggt ttggtggagc gtcatcgtaa
     7201 tcctgaaaat cagcgggaag tgttggtaga gactgcgaag tatgccaagg agcagttagt
     7261 ggtgaatccc cctctgcaac atatcaaggt taaggaagag atggaaagta tcttaacaga
     7321 gtttgagaga acagaactca gccgtttatt aaataaattg gttttgggta ttgaaaatat
     7381 agaaatttaa ggagaaatag atgtcaatta ttacaattat tttaacaacg atcgttgctt
     7441 tggagcattt ttacattttt tatttggaaa gtattgccac gcaatcagat gcgactagtc
     7501 gtgtatttaa tatggaaaag gaagaattgg ctcatccgtc agtaagttca ttgttcaaaa
     7561 atcaagggat ttataaggct ctgctaggag tctttctctt gtatgtcatt tatttctcac
     7621 agaatttaga aattgtgact atttttgtct tatttgtgat tggtgctgcg acttacggct
     7681 ctttaacagc ggataaaaaa attattttga aacaaggtgg atcagctatt ttggccttga
     7741 ttagtatttt actctttaaa tacacttgaa ggtcgattct aatctcgcta atccttttta
     7801 atccagaata agggaaatat gttatacttg tttttaagaa aaaagtttca ttgaattggt
     7861 tttgaggagt tagaaatgaa agtattagtg acaggttttg agcccttttg aggccattaa
     7921 aggtttacca gctgaaatcc atggtgctga ggtccgttgg ctagaggtgc cgacagtttt
     7981 tcacaaatct gctcaagtat tggaagaaga gatgaatcgt tatcaacctg actttgtcct
     8041 ttgtattggg caagctggtg gaagaactag tttgacacct gaacgagtgg ccattaatca
     8101 agacgatgca cgcatttctg ataacgaaga taatcaaccg attgaccgtc ccattcgccc
     8161 agatggtgct tcggcctact ttagtagttt gccgattaaa gcgatggttc aagctataaa
     8221 aaataaggat taccggcctc tgtttccaat acggcaggga cttttgtctg cagccatttg
     8281 atgtatcagg ctctctattt ggtagaaaag aaatttccat atgttaaggc aggttttatg
     8341 catattcctt atatgatgga acaggtggtg aacagaccga ctactccaac tatgagttta
     8401 gtggatattc ggcgagggat agaagcagca atcggcgcta tgaatagaac atggagatca
     8461 ggaactcaag ttggtaggcg gagaaattca ttgatagaaa aaagcttgag gggaaaacct
     8521 tcaagctttt ggacgttttc gagccaatac tgctcggtaa aacataattt tagtgcattg
     8581 gatataaggt aggagtgaaa aactagcaat gccaaaggta atccaattga ggaagtacca
     8641 aggaagaagc tgtaaatcta ggacaaagtg ctggaacttg tagcccttca taaaggaacg
     8701 gctagttttt aggattcgtc ttggtgggac ctgtcctagg tctaaactat aacagagaag
     8761 aaattccacc tgtgaatagg cataatactg tggaatatag aggatatttc ctacaatgat
     8821 caagatgaga cttgcaagaa agtagagtcc aaagaccatg aggaaacgct cggtttcaac
     8881 tgatgagaga tctagatttg gaaactcagg atgtagggtg acgaattttt tggctaaaaa
     8941 gctactataa aagaggaggt aaatcccaag taaattaggg atactccata aaaagagata
     9001 gaaacgtttg agaagtaggg tcaaaaaggt ttgagaaaag cgctcctcat caaagagagc
     9061 taggctgttt tttacagatg gctccgtttt agaatctttc atgagtgtca gtgttgcata
     9121 gacggaactg gtcaaaagaa tagtcccgat aaaggagact agtagaggaa agaggtaggt
     9181 ttgaagtatt tggccaagta tgctgaaaaa tggctgttct aaaacagtcc cgtggatccg
     9241 agataaggga ttaagaaaac cagataagat gaccagcata ctgggaagga tatagaggag
     9301 aaagagacgg ggggtgtcag cctgaaaatg ttttgactcc tgacgaattg tttttaaatc
     9361 aatttttgga tagttcattc tcttattata ccatagttct tatacatagt tcgtgacagt
     9421 tcctactttt tttgataaaa tcatacagtg tgtccttggg cacactgtat gaactgggac
     9481 tgtctttccc agcttcggag gtaaaaaatg tcagattcac caatcaaata tcgtttgatt
     9541 aagaaagaaa aacacacagg agctcgtcta ggagaaatca tcactcccca cggtaccttt
     9601 ccgacaccta tgtttatgcc agttgggaca caagccactg tcaaaactca gtcacctgaa
     9661 gaattgaagg agatgggttc gggaattatc ctatcaaaca cctatcatct ctggcttcgc
     9721 cctggagatg aactcattgc acgcgctggt ggtctccaca agttcatgaa ttgggaccag
     9781 cctatcttga cagatagtgg tggttttcag gtttattctt tagcagatag ccgtaatatc
     9841 acagaagaag gagtaacctt taaaaatcat ctaaatggtt ctaagatgct cctatcccca
     9901 gaaaaagcca tctctattca gaataatctg agttcagaca tcatgatgtc ctttgatgaa
     9961 tgtcctcagt tttatcaacc ttatgactac gttaagaaat cgatcgagcg taccagccgt
    10021 tgggctgagc gtggtttgaa ggctcaccgt cgtccacatg accaaggttt gtttggaatt
    10081 gtgcaaggtg caggatttga agaccttcgc cgccaatcag ctcacgatct tgtcagcatg
    10141 gatttctcag gctactctat cggtggtttg gcagtgggag aaacccatga agagatgaat
    10201 gcggtcttgg actttacaac tcaactgctg cctgaaaata aacctcgtta tctgatgggt
    10261 gtgggagcgc cagatagctt gatcgatggg gtcattcgtg gggtggatat gtttgactgt
    10321 gtcttaccga ctcgaattgc tcgtaacggg acttgtatga ccagtcaagg acgtttggtt
    10381 gtgaaaaatg cccagtttgc tgaggacttt acgccactgg atcctgagtg tgattgctac
    10441 acatgtaata actatacacg cgcttacctt cgtcacctgc tcaaggctga tgaaaccttt
    10501 ggtatccgct tgactagcta ccacaatctt tacttcttgc ttaacctgat gaagcaagtg
    10561 cgacaagcca tcatggatga caatctcttg gaattccgta agtattttgt ggaaaaatat
    10621 ggctataata agtcaggacg taatttctaa aatggaattg atataagcta aaaatcctaa
    10681 gttttctctt aggatttttc ttcttttttt gatagaataa agtgtacaat gaaaggaaga
    10741 ataaactcgt atgcgcatta aatggttttc cttgattagg attataggtt tacttttggt
    10801 actcttgtat cacttctttc agacgatctt tcctggagga tttttcgggg tagatgtctt
    10861 tttcacattt tcaggtttcc tgattacagc tctactcatt gaagaatttt ctaagaacaa
    10921 tgagattgat ttgataggat tttttagaag acgcttttat cggattgtgc cacctgtggt
    10981 tttgatggtc ttggtgacca tgccttttac tttcttggtt cgccaagact atgttgctgg
    11041 aattggtggc cagattgcgg gcgtcttagg ctttatgacc aacttctatg aactcttaac
    11101 aggtgggagt tatgaatctc agttcattcc tcatttgttt gttcataatt ggagtttggc
    11161 agttgaggtt cactactata ttctttgggg attggcagtt tggttcttat ccaaacaagc
    11221 taaatcaaat ggtcagttga aggggatgat ctttctctta tctgctactg ccttcttgat
    11281 cagtttcttc tccatgttta ttggtagttt tctagtgacc tcttattcct ctgtttattt
    11341 ctccagttta actcatgtct atccattctt tttgggaagt atgttagcaa ctattgtagg
    11401 cgttcgtcag acgacttccc tcgtcaagca gttggataaa atctgggatt tacgaaagac
    11461 tttggtagtt tttggaggag gctttggttt cctagttctt ttgactttct ttgtcaaatt
    11521 cacttatctt tttgcctatc ttatcggctt cttgcttgcc agtcttgcag ctcttgccat
    11581 gattctggcg gcgcgtgtct tacatgaaaa gacacatcat atacaggagc cgaagattat
    11641 cagcttttta gcggatacta gctatgcggt ttatcttttc cattggcctt tctatatcat
    11701 tttctcacag ttgacatcaa atcttcttgc tgtattactg actctgattt gttcttatgg
    11761 ctttgccagt ctgtcatttt atgtattgga accttggatt gcaggcaaga acacacctat
    11821 tgtccaaacc cttcgtcccc tgccttatat tcacgcaatt cttgcagcag gtacaggaat
    11881 cttgaccttc attgtctgca tggtgacctt gttggcacca caagtgggag cgtttgagac
    11941 agacttgact gtcaatggct tgaagcaagc tgcaacaaat attggccaga ccaaggtgat
    12001 ggcagaacgg gcagatgcaa acagtttggg aattgctgat ggcactatgt taattggtga
    12061 ctcagtggct ttaagggcaa atacagcact acagacagct cttcctggag cacagattaa
    12121 cgcgcaggtc agcgtaacaa ccaagaccgc aaatgaaatc atgctaaata atagccagaa
    12181 taaattttta cctaagacgg tggtcattgc gactggggta aataatcctg agaactacaa
    12241 ggatgactgg gacagtatcg tgaaaaatct tcctaaggga caccatatga ttttggtgac
    12301 tccttatgag ggagataaga caaaagagac ctatgccatc gttgagaagg ctgctgccta
    12361 tatgagagaa ttggcagaga agacacctta cattacgata gcagattgga atcaagttgc
    12421 gaaagagcat ccagaaattt gggctggaac agatcaggtt catttcggga gtgagagtag
    12481 cactatcgaa gcaggagcaa aattgtatgc agatacgatt gccacagctt tgcagacagc
    12541 tcaagacaag ccggttaaat caaaataact tgtattaaaa aagagaagag ttggagaaat
    12601 ccagctcttt taaaatatct ccaaaaaata ggtatatacc atttacaaat aaaaaagaaa
    12661 ggtttataat gtaattgata taacaaattg tagaatcaat cttttaagga ggttaacatt
    12721 atgcctaact atattaaagc ggatcagttt ttctacccac acggagttcg tcgaggtggt
    12781 tacttggaac ttgtggacgg caagtttgga aaacatgtag agcagattcc tgaaggggct
    12841 gaggtgattg actatacagg ttatagcatt gccccaggtc ttgtggatac tcatattcat
    12901 ggatatgcag gtgtagatgt gatggacaac aacattgaag gtacattgca tactatgagt
    12961 gaaggacttc ttagtaccgg tgttaccagt ttcttaccca caactttaac agccacttat
    13021 gagcaattgc ttgcagtcac tgaaaatctt ggaaaccatt ataaagaagc aacaggtgct
    13081 aagattcgtg ggatttatta tgaaggtcca tatttcacag aaacttttaa gggggcacaa
    13141 aatccaactt atatgagaga cccgggtgtt gaggagtttc attcttggca aaaagcggca
    13201 aatggcttgc ttaataaaat tgcccttgca ccagaacgtg atggggtgga agactttgtt
    13261 cgtacagtta cgggcgaagg tgtgacggtt gctcttggac attcaaacgc gacttttgat
    13321 gaagccaaaa aagcagtcga tgctggagcg agtgtttggg tgcatgccta caatggaatg
    13381 cgtgggttga ctcaccgtga attgggtatg gttggagcca tgtaccaatt gccacatacc
    13441 tatgcagagt tgatctgtga tggtcaccac gtagatccaa aggcttgcga aattcttatc
    13501 aaacaaaaag gaacagaaaa catcgctctt atcacagact gtatgacagc tgggggattg
    13561 gaagacggag attatatgtt gggagaattc ccagttgtcg ttgcaaatgg aactgcacgc
    13621 ctcaaatcga caggtaactt ggcaggttct atcctcaaac tcaaagatgg tttgaagaat
    13681 gtggtcgaat ggggaattgc gaatccgcat gaagcagtca tgatggccag cttcaaccca
    13741 gctaaatccg ttcacatcga tgacgtctgt ggccaaatcc gtgaaggcta cgacgctgac
    13801 ttcatcgtat tagataaaga tttggaattg gtagcaacct acctagatgg cgtgaaacgt
    13861 tatcaagcat aagagagaaa gcagaagtta gagactaact tctgcttttt tatgcagggt
    13921 actttactgg taaataaaaa gttaaaaaaa tcacaaaaaa agcttgaaga aacaaagaaa
    13981 aaggcttaca attgaactat aaatagtaca aataaaaaaa cggaggagtg ctttatgaaa
    14041 gcttatactt atgttaaacc aggacttgct tcttttgttg atgtagacaa accagttatt
    14101 cgcaagccaa cagacgctat tgtgcgtatt gtaaaaacca ctatttgtgg aacagacctc
    14161 catattatca aaggggatgt tcctacttgc caaagtggta ccattcttgg ccacgaaggg
    14221 attgggattg ttgaagaagt tggggaagga gtttccaact tcaaaaaagg tgacaaggtc
    14281 ttgatttctt gcgtctgtgc ctgtggtaaa tgctactact gtaaaaaagg aatttatgcc
    14341 cactgtgaag acgaaggggg ctggattttc ggtcacttga ttgatggtat gcaggctgaa
    14401 tatctacgtg tccctcatgc agataatact ctttaccata ctccagaaga cttgtcagat
    14461 gaagctttgg ttatgctgtc agacattctg cctactggat atgaaattgg tgtcttaaaa
    14521 gggaaagtag aacctggttg cagcgtagcc attattggtt caggtccagt tggattggct
    14581 gctcttttaa cagcccaatt ctattcacca gctaaattga ttatggtaga cctagacgat
    14641 aaccgcttgg aaactgccct atcattcggt gcgactcata aggttaattc ttcagaccct
    14701 gaaaaagcca ttaaagaaat ttatgatttg acagatggtc gtggtgtgga tgtcgctatc
    14761 gaagctgttg gtattcctgc aacatttgat ttctgtcaaa agattatcgg tgtagacgga
    14821 acggttgcca actgtggtgt gcatggtaaa ccagttgaat tcgatttaga taaactttgg
    14881 attcgcaaca tcaatgtaac aactggtttg gtatctacaa atacgactcc acaattgttg
    14941 aaagcacttg aaagtcataa gattgaacca gaaaaattgg taactcacta tttcaaactc
    15001 agtgaaattg aaaaagccta cgaagtcttc agtaaggcag cagaccacca tgccattaag
    15061 gtcattatcg aaaacgatat ctcagaagcc taagtagtaa aaatattttt gtacataagt
    15121 aaatagaaat tcagtcatcc atcagatggc tggatttttt atcaaaaaat tcagaaatga
    15181 gcatatttct ttccttgtct ggcggaattg gttataatat acggtacaaa ggaatgaatg
    15241 aatatgtatc gtgttataga aatgtacgga gattttgaac cgtggtggtt cttagaaggt
    15301 tgggaagaag atattgtagc aagtagaaaa tttgatcagt attatgatgc tctcaaatac
    15361 tacaaaactt gctggtttag attggaacaa gaatcccctc tttataaaag tagaagcgat
    15421 ttgatgacca ttttttggga cccggaagac caacgctggt gtgatgaatg tgatgagtat
    15481 ttacaacaat accattcttt ggctcttttg caggatgagc aggttatccc agacgaaaaa
    15541 ctacgctcag gctatgaaaa acaaaccagt caggaaagga atcgttcttg ccgtatgaaa
    15601 ttaaaataga gaaaagtaac ttttttggag ttgctttttt tatttttcta actctttgcg
    15661 aatagtatag gtgaggaggt aagtatggtt caagaaattg cacaagaaat cattcgttca
    15721 gctcgggaaa aagggacgca ggatatttat tttgtcccta agttagacgc ctatgagctt
    15781 catatgaggg taggagacga gcgctgtaaa attggtagct atgattttga aaagtttgca
    15841 gccgttatca gtcactttaa gtttgtggcg ggtatgaatg tgggagaaaa aagacgtagt
    15901 caactgggtt cctgtgatta tgcctatgac cagaagatgg cgtctctacg tttatctact
    15961 gtaggcgatt atcgggggca tgagagtttg gttatccgtt tgttgcacga tgaggaacag
    16021 gacttgcatt tttggtttca ggatattgaa gaattaggca agcagtacag gcaacgggga
    16081 ctctatcttt ttgctggtcc ggttgggagt ggtaagacga ccttgatgca tgaattgtcc
    16141 aagtcactct ttaaaggaca gcaagttatg tccatcgaag atcctgtcga aatcaagcag
    16201 gacgacatgc ttcagttgca gttgaacgaa gcaatcggcc taacctatgg aaatctaatc
    16261 aaactttcct tgcgtcatcg accagatctc ttgattatcg gagaaattcg tgacagcgag
    16321 acggcgcgtg cagtggtcag agctagtttg acaggtgcga cagtcttttc aaccattcac
    16381 gccaagagta tccgaggtgt ttatgagcgt ctgctggagt tgggtgtgag tgaagaagaa
    16441 ttggcagttg ttctgcaagg agtctgctac cagagattaa tcgggggagg aggaatcgtt
    16501 gactttgcaa gcagagatta tcaagaacac caagcagcca agtggaatga gcaaattgac
    16561 cagcttctta aagatggaca tatcacaagt cttcaggctg agacggaaaa aattagctac
    16621 agctaagcaa aaaaatatca tcaccctatt taacaatctc ttttctagcg gttttcatct
    16681 ggtggagact atctcctttt tagataggag tgctttgttg gacaagcagt gtgtgaccca
    16741 gatgcgtgtg ggcttgtctc aggggaaatc attctcagaa atgatgaaaa gtttgggatg
    16801 ttcaagtgct attgtcactc agttatccct agctgaagtc catggtaatc tccacctgag
    16861 tttgggaaag atagaagagt atctagacaa tctggccaag gtcaagaaaa aattaattga
    16921 agtagcgacc tatcctttga ttttgctgtt ttttcttctc ttaattatgc tggggctacg
    16981 gaattacctg ctcccacaac tggatagtag caatattgcc acccgaatta tcggtaatct
    17041 gccacaaatt tttctaggca tggtagggct tgtttccgtg cttgcccttt tagcactaac
    17101 tttttataaa agaagttcta agatgagtgt cttttctatc ttagcacgcc ttccctttat
    17161 tggaatcttt gtgcagacct acttgacagc ctattatgca cgtgaatggg ggaatatgat
    17221 ttcacaggga atggagctga cgcagatttt tcaaatgatg caggaacaag gttcccagct
    17281 ctttaaagaa atcggtcaag atctggctca aaccctgaaa aatggccgtg aattttctca
    17341 gacgatagga acctatcctt tctttaggaa ggaattgagt ctcatcatag agtatgggga
    17401 agttaagtcc aagctgggta gtgagttgga aatctatgct gaaaaaactt gggaagcctt
    17461 ttttacccga gtcaaccgca ccatgaattt ggtgcagcca ctggttttta tttttgtggc
    17521 actgattatc gttttacttt atgcggcaat gctcatgccc atgtatcaaa atatggaggt
    17581 aaatttttaa aatgaaaaaa atgatgacat tcttgaaaaa agctaaggtt aaagctttta
    17641 cattggtgga aatgttggtg gtcttgctga ttatcagcgt gcttttcttg ctctttgtac
    17701 ctaatctgac caagcaaaaa gaagcagtca atgacaaagg aaaagcagct gttgttaagg
    17761 tggtggaaag ccaggcagaa ctttatagct tggaaaagaa tgaagatgct agcctaagaa
    17821 agttacaaga cgatggacgc atcacggaag aacaggctaa agcctataaa gaataccatg
    17881 ataaacaaaa tactagtcaa atcgttaaag attaaggcct ttaccatgct ggaaagtctc
    17941 ttggttttgg gacttgtgag tatccttgcc ttgggcttgt ccggctctgt ccagtccact
    18001 tttgcagcgg tagaggaaca gattttcttt atggagtttg aagaactcta tcgggaaacc
    18061 caaaaacgca gtgtagctag tcaacaaaag actagtctga acttggatgg gcagatgatt
    18121 agcaatggca gtcaaaagtt gccagtccct aaaggaattc aggccccatc aggccaaagt
    18181 attacatttg accgtgctgg gggcaattcg tccctggcta aggttgaatt tcagaccagt
    18241 aaaggagcga ttcgctatca attatatcta ggaaatggaa aaattaaacg cattaaggaa
    18301 acaaaaaatt agggcagtga ttttactgga agcagtagtc gctctagcta tctttgccag
    18361 cattgcgacc ctccttttgg gacaaattca aaaaaatagg caagaggaag caaaaatctt
    18421 gcaaaaggaa gaagtcttga gggtagctaa gatggccttg cagacggggc aaaatcaggt
    18481 aagcatcaac ggagttgaga ttcaggtatt ttctagtgaa aaaggattgg aggtctacca
    18541 tggttcagaa cagttgttgg caatcaaaga gccataaggt caaggctttt accttgttag
    18601 aatccctgct tgccctcatt gtcatcagtg ggggattact cctttttcaa gctatgagtc
    18661 agctcctcat ttcagaagtt cgctaccagc aacaaagcga gcaaaaggag tggctcttgt
    18721 ttgtggacca acttgaggta gaattagacc gttcgcagtt cgaaaaagta gaaggcaatc
    18781 gcctatacat gaagcaagat ggcaaggaca tcgccatcgg taagtcaaag tcagacgatt
    18841 ttcgtaaaac cgatgctagt ggacgggggt atcagcctat ggtttatggc ctcaaatcag
    18901 ctcagattac agaggacaat caactggttc attttcgttt ccagtttcaa aaaggcttag
    18961 aaagggagtt catctatcgt gtggaaaaag aagaaagtta aggcaggtgt tctcctctat
    19021 gcagtcacca tggcagctat ctttagtctt ttgttgcaat tttacttgaa ccgacaagtc
    19081 gcccactatc aagactatgc tttgaataaa gaaaaattgg ttgcttttgc tatggctaaa
    19141 cgaaccaaag ataaggttga gcaagaaagt ggggaacagg tttttaatct aggtcaggta
    19201 agctatcaaa acaagaaaac tggcttagtg acgagggttc gtacggataa gagtcaatat
    19261 gagtttctgt tttcttcagt caaaatcaaa gaagagaaaa cggataaaaa ggaagaggta
    19321 gtgactgatt caagcgaaaa agcggagaaa aaaaatcaga agagaagcct gaaaagaaag
    19381 agaattcata gtcaattcaa ctataatgcg ttgaatccag aatagtccac tgtagtttct
    19441 agaaaattgc tggaaatgga tgttaatctc caattcattt gtttatatct tatttcagtt
    19501 tactatactt tgtgctaaac taaagatatg aaacatgatt ttaaccacaa agcagaaact
    19561 ttcgattccc ctaaaaatat cttcctcgca aacttggtat gtcaagcagc cgagaaacag
    19621 attgatcttc tatcagacaa agaaatttta gatttcggtg gtggcgggtc tattagcctt
    19681 gcccctaacc cctagccaag caggctaagt cagtcactct tgtagacatt tctgagaaaa
    19741 tgttggagca agctcgtttg aaagtggagc agcaagcaat caagaatatc cagtttttgg
    19801 agcaagattt accgaaaaat cccttggaga aagagtttga ttgccttgct gttagtcggg
    19861 ttcttcatca tatgcctgat ttggatgcgg ctctctcact gtttcatcaa catttgaagg
    19921 aagatgggaa actcatcatt gctgatttta ccaagacaga agctaatcat catggatttg
    19981 atttagctga actggaaaac aagctaattg agcatggttt ttcatctgtg catagtcaga
    20041 ttctctatag cgctgaagac ctgtttcaag gaaatcactc agaattcttt ttaatagtag
    20101 cccaaaaatc actcgcctag tcagggagtg atttttctat aaggatggaa aaaagaaggg
    20161 aaatttgata agataggaat atggattttg aaaaaattga acaagcttat acctatttac
    20221 tagagaatgt ccaagtcatc caaagtgatt tggcgaccaa cttttatgac gccttggtgg
    20281 agcaaaacag catctatctg gatggtgaaa ctgagctaaa ccaggtcaaa gacaacaatc
    20341 aggcccttaa gcgtttagca ctacgcaaag aagaatggct caagacctac cagtttctct
    20401 tgatgaaggc tgggcaaaca gaacccttgc aggccaatca ccagtttaca ccggatgcta
    20461 ttgctttgct tttggtgttt attgtggaag agttgtttaa agaggaggaa attactatcc
    20521 tcgaaatggg ttctgggatg ggaattctag gcgctacttt cttgacctcg cttgataaaa
    20581 aggtggatta cttgggaatg gaagtggatg atttactgat tgatctggca gctagcatgg
    20641 cagatgtaat tggtttgcag gctggctttg tccaaggaga tgccgttcgt ccacaaatgc
    20701 tcaaagaaag cgatgtggtc atcagtgact tgcctgttgg ctattatcct gatgatgccg
    20761 ttgcgtcgcg ccatcaagtt gcttctagcc aagaacatac ttacgcccat cacttgctca
    20821 tggaacaagg gcttaagtac ctcaagtcag acggatacgc tatttttcta gctccgagtg
    20881 atttgttgac cagtcctcaa agtgatttgt taaaagaatg gctgaaagaa gaggcgagtc
    20941 tggttgctat gattagtctg cctgaaaatc tctttgctaa tgctaaacaa tctaagacta
    21001 tttttatcct acagaagaaa aatgaaatag cagtagagcc ttttgtttat ccacttgcta
    21061 gcttgcaaga tgcaagtgtt ttaatgaaat ttaaagaaaa ttttcaaaaa tggactcaag
    21121 gtactgaaat ataaaataga ttttgttata atagttgaaa acgcttaaaa aggggtatca
    21181 tgttatgaca aaaacaattg caatcaatgc aggaagttca agtttgaaat ggcaattata
    21241 cttaatgcca gaagaaaaag tattggcgaa aggtttgatt gaacgtatcg gtttgaaaga
    21301 ttcaatttca actgtaaaat ttgacggccg ttctgaacaa caaattttgg atattgaaaa
    21361 tcatacacaa gccgttaaaa ttttattgga tgacttgatt cgtttcgata ttatcaaggc
    21421 ttatgacgag attacaggtg ttggacatcg tgttgttgct ggtggagaat atttcaaaga
    21481 atcaacagtt gttgagggag atgttttaga aaaagttgaa gagttgagtt tgttggctcc
    21541 tctacacaac ccggccaatg cagcaggtgt tcgtgccttc aaggaattgt tgccagacat
    21601 taccagtgta gttgtttttg atacttcctt ccacacaagt atgccagaga aagcttatcg
    21661 ctaccctcta ccaacaaaat attacacaga aaacaaggtt cgtaaatacg gtgctcatgg
    21721 tacaagtcac cagtttgtag caggagaagc tgcaaaactc ttgggacgtc cattagaaga
    21781 cttgaagtta attacctgtc atattggtaa cggaggctca attacagctg tgaaagccgg
    21841 caaatctgta gacacttcta tggggttcac tcctcttggt ggtattatga tgggaacgcg
    21901 tacaggggat attgatccag ctatcattcc ttatttaatg caatatacag aggattttaa
    21961 cacaccagaa gatatcagtc gtgttcttaa ccgtgaatca ggtcttttgg gagtttctgc
    22021 taattctagc gatatgcgcg atatagaagc agctgtagca gaagggaatc acgaggctag
    22081 cttggcttat gaaatgtatg ttgaccgtat ccaaaaacat atcggtcagt accttgcagt
    22141 gctaaatgga gcagatgcca ttgttttcac agcaggtgtc ggtgaaaatg cagagaattt
    22201 ccgtcgtgat gtaatctcag ggatttcgtg gtttggttgt gatgttgatg atgaaaagaa
    22261 tgtctttggc gttacaggag acatctcaac agaggcagct aaaatccgtg tcttggttat
    22321 tccaacagat gaagaattag tcattgcccg tgacgttgaa cgcttgaaaa aataagtgaa
    22381 actaaaaaaa tattcaatac aaggagttgg gaaagttatt tttccagctt ctttttctga
    22441 tgaaattgtc caaaaccttg ctatgattgg cttttttgaa aaatatggta taatagtagt
    22501 aatttaatag atggagttga gttttgaaga aaaactttcg tgtaaaaaga gagaaagatt
    22561 ttaaggcgat tttcaaggag gggacaagtt ttgctaatcg caaatttgtg gtctaccaat
    22621 tagaaaacca gaaaaaccat tttcgagtag gtctatcagt tagcaaaaaa ctggggaatg
    22681 ccgtcactag aaatcaaatt aagcgacgga ttcggcatat tatccagaat gcaaaaggga
    22741 gtctggtaga agatgtcgac tttgttgtca ttgctcgaaa aggagtcgaa accttgggat
    22801 acgcagagat ggagaaaaat ctactccatg tattaaaatt atcaaagatt taccgggaag
    22861 gaaatgggag tgaaaaagaa actaaagttg actagtttgc taggactgtc tctgttaatc
    22921 atgacagcct gtgcgactaa tggggtaact agcgatatta cagccgaatc ggctgatttt
    22981 tggagtaaat tggtttactt ctttgcggaa atcattcgct ttttatcgtt tgatattagt
    23041 atcggagtgg ggattattct ctttacggtc ttgattcgta cagtcctctt gccagtcttt
    23101 caggtgcaaa tggtggcttc taggaaaatg caggaagctc agccacgcat taaggcgctt
    23161 cgagaacaat atccaggtcg agatatggaa agcagaacca aactagagca ggaaatgcgt
    23221 aaagtattta aagaaatggg tgtcagacag tcagactctc tttggccgat tttgattcag
    23281 atgccggtta ttttggccct gttccaagcc ctatcaagag ttgacttttt aaagacaggt
    23341 catttcttat ggattaacct tggtagtgtg gatacaaccc ttgttcttcc gattttagca
    23401 gcagtattca cctttttaag tacttggttg tccaacaaag ctttgtctga gcgaaatggc
    23461 gctacgactg cgatgatgta tgggattcca gtcttgattt ttatctttgc agtttatgcg
    23521 ccaggtggag tcgccctata ctggacagtg tctaatgctt atcaagtctt gcaaacctat
    23581 ttcttgaata atccattcaa gattatcgca gagcgcgagg ccgtagtaca ggcacaaaaa
    23641 gatttggaaa atagaaaaag aaaagccaag aaaaaggctc agaaaacgaa ataaataagg
    23701 aggaatctgg taatggtagt atttacaggt tcaactgttg aagaagcaat ccagaaagga
    23761 ttgaaagaat tagatattcc aagaatgaag gctcatatca aagtcatttc tagggagaaa
    23821 aaaggctttc ttggtctatt tggtaaaaaa ccagcccaag tggatattga agcgattagt
    23881 gaaacgactg ttgtcaaagc aaatcaacag gtagtaaaag gcgttccgaa aaaaatcaat
    23941 gatttgaacg agcctgtgaa gactgttagt gaagaaaccg ttgaccttgg tcatgtggtt
    24001 gatgctatta aaaaaataga ggaagaaggt caaggtattt ctgatgaagt caaggctgaa
    24061 atcttaaaac atgaaagaca tgccagcact atcttagaag aaactggtca cattgagatt
    24121 ttaaatgaac ttcaaatcga ggaagcgatg agggaagaag caggcgctga tgaccttgaa
    24181 actgagcaag atcaaactga aaatcaagac ttgaaagaga tgggcttgaa ggtcgagcaa
    24241 agttatgata ttgcccaggt ggctacggat gtgactgctt atgttcaaac gattattgat
    24301 gacatggatg ttgaggctac actttcaaat gattataacc gtcgtagcat caatttgcaa
    24361 attgatacta acgaaccagg tcgtattatc ggctaccatg gtaaagtctt gaaggccttg
    24421 caactgttgg ctcaaaatta tctttacaac tgctattcaa gaaccttcta tatcactatt
    24481 aatgtcaatg attatgttga acaccgtgca gaagtcttgc agacctatgc gcaaaaattg
    24541 gcgattcgtg ttttagaaga aggacgtagt catcaaacag atccaatgtc gaatagtgaa
    24601 cgcaagatta tccaccgtat tatttcacgt atggatggcg tgaccagtta ctctgaaggt
    24661 gatgagccaa atcgctatgt cgttgtagat acagaataag taaaatcagg gttgacctga
    24721 ttttttgcta gctagaggag gttaaactga tgttgaataa gataagagac tatctagact
    24781 tttctggttt gcagtaccgt agtcctgata aagcgggagc agagcgagag aagatgctgg
    24841 cgttccgcca caaaggacaa gaggcccgaa aggcttttac agaactggcc aaagcctttc
    24901 aagcaagcca tccagaatgg caactccaac agactagtca gtggatgaat caggcccagc
    24961 gtttgagacc acatttttgg gtttatctac agagagacgg acaagtgaca gaacctatga
    25021 tggccttacg tttgtatggg acatctactg actttggaat ttctttggaa gtcagtttca
    25081 tcgaacgtaa gaaggatgag caaacactgg gcaagcaggc caagatttta gacattccaa
    25141 ccgttaaagg gatttattat ctaacctact ctaatggtca aagtcaacgg tgggaggcga
    25201 atgaagaaaa gcgtcgtact ttacgcgaga aggtgagaag tcaagaagtt cgaaaagttt
    25261 tagtgaaggt agatgttcct atgacagaaa attcgtctga agaagaaatc gtagaaggct
    25321 tattgaagtc ttattctaaa attcttccct attatctagc tacgagaaaa taagataatt
    25381 tgtaaaacat cataaatcat acagtccaag agtgaacagt ccgctgtgta attcttggtc
    25441 tttttgtttg cgctttcgca ttatataata aacttacaaa aacaattcaa aaggagaaca
    25501 attatggaag tcgtttcaag tgttctaaat tggttttcta gcaatatttt gcagaatccc
    25561 gcatttttcg taggtttatt ggtgttgata ggatatgcac ttttgaaaaa acctgcccat
    25621 gacgtttttt cagggtttgt taaagcaaca gtagggtata tgttgcttaa cgtgggtgct
    25681 ggtggtttgg ttacaacctt tcgtccaatc ttagcagctc ttaactacaa attccaaatt
    25741 ggtgcagcgg ttatcgaccc ttactttgga cttgctgcag caaacaacaa aattgcagca
    25801 gagtttccag attttgttgg aactgcaact acagctctat tgattggttt cggaataaat
    25861 atcttgctcg tagctcttcg aaagattacg aaggtaagaa ccctctttat tactggtcac
    25921 atcatggtac aacaagctgc aacagtatct cttatggttc tattcttagt accacaattg
    25981 cgcaatgctt acggtacagc agcgattggt atcatctgtg gactttactg ggcagttagt
    26041 tcaaatatga ctgttgaggc aactcaacgc ttgactggtg gtggcggatt tgcgattggt
    26101 caccaacagc aatttgcaat ctggtttgta gataaagtag caggacgctt tggtaagaaa
    26161 gaagaaagtt tagacaatct taaattacct aagttcctct caatcttcca cgatacagtt
    26221 gttgcatctg ctaccttgat gctcgtattc ttcggggcca ttcttttaat cttgggtcca
    26281 gacattatgt ctaataaaga agtcatcact tcaggaactc tattcaatcc tgctaaacaa
    26341 gatttcttta tgtacattat ccaaacagcc tttaccttct cagtttactt gttcgttttg
    26401 atgcaaggtg tccgaatgtt cgtatctgag ttaacaaacg ctttccaagg tatttcaaac
    26461 aaattgttgc caggttcatt cccagcggtt gacgttgcag cttcttatgg atttggttct
    26521 ccaaatgctg tcttgtcagg atttaccttt ggtttgattg gtcaattgat tacaattgtc
    26581 ttgctcatcg tctttaaaaa tccgattctt attattacag gatttgtacc agtgttcttt
    26641 gacaatgcag ccattgcggt ctacgctgat aaacgcggcg gatggaaagc ggctgttatc
    26701 ctttccttta tatcaggtgt ccttcaagtt gctctaggag ctctttgtgt ggcccttctc
    26761 gatttggcat cttatggtgg ctaccatgga aatatcgact ttgaattccc atggcttgga
    26821 tttggatata tcttcaaata ccttggtatt gttggttatg tacttgtgtg tctcttcttg
    26881 cttgttattc ctcaacttca atttgccaaa gcaaaagata aagagaaata ttacaacggt
    26941 gaagttcaag aagaagctta gtatctagaa aaggagaaat aaaatggtta aagtattagc
    27001 agcgtgcgga aatggaatgg gttcatcaat ggttatcaag atgaaggttg aaaatgctct
    27061 ccgtaagctt aatcaaacag attttacagt caattcatgc agtgtcggtg aagctaaagg
    27121 tttagcagta ggatatgaca tcgtaatcgc ttctcttcat ttgattcaag aattggaagg
    27181 gcgaactaat gggaagttaa ttggtcttga taacttgatg gatgataaag aaatcaccga
    27241 aaaactcagt caagcactat agtaaaaggt tggaggggac tggacagaaa ctgagagtta
    27301 tcgtttctgt ccttctccct ctttaaataa aggaggcaga tatgaattta aaacaagctt
    27361 taattgacaa cgactcgatc cgactaggtt tagaggctaa cgattggaaa gaagcagtca
    27421 aggtagcagt agatcccttg attgaaagtg gggcaatttt gccagagtat tacgatgcta
    27481 tcattgaatc gactgaagag tatgggcctt actatatctt gatgccaggt atggctatgc
    27541 cccacgctag acctgaagct ggtgtgcaaa gtgatgcctt ttcattgatt accttacaaa
    27601 atcctgttgt attttcagat gggaaagagg tatctgtttt gttggcacta gcagcaacaa
    27661 gttcaaaaat tcacacaagt gtggccattc cacaaattat tgccctgttt gaattagaag
    27721 attctattgc acgtttacag gcttgccaga ctaaagaaga tgtcttggct atgattgaag
    27781 aatctaagga tagcccttat ctcgaaggat tggatttgga aagttagaaa gaggaataaa
    27841 gaaatgacaa aaagaatacc taatttacaa gttgcattag accattcaga cttgcaagga
    27901 gcgattaaag cagctgtttc tgttggtcag gaagtagata ttatcgaagc tggaactgtt
    27961 tgcttgcttc aagttggaag tgaactggct gaagtcttgc gtagcctttt cccagataag
    28021 attattgtgg cagacacaaa atgtgctgat gctggtggaa cagttgctaa aaataatgcg
    28081 gttcgtggag cagactggat gacttgtatc tgttgtgcaa ccatccctac tatggaagca
    28141 gctctaaagg ctatcaagac tgaacgagga gaacgaggcg aaatccagat cgagctttat
    28201 ggcgattgga cttttgaaca agctcagctt tggctagatg caggtatctc acaagctatt
    28261 tatcaccaat ctcgtgatgc tcttcttgct ggtgaaactt ggggtgaaaa agaccttaat
    28321 aaggttaaaa aactcattga catgggcttc cgtgtatctg taacaggtgg tctagatgta
    28381 gatactctca aactctttga aggtgttgat gtctttacct ttatcgcagg tcgtagaatt
    28441 acagaggctg cggatccagc aggagcagcg cgtgccttca aggatgaaat caaacgaatt
    28501 tgggggtaaa tcatggtacg tccaattgga atttatgaaa aggcaacccc aacacacttt
    28561 acttggctag aacgtttaaa ttttgccaag gagttaggct ttgattttgt cgagatgtct
    28621 attgacgaac gtgacgagcg tttagcaaga cttgactgga gtaaggaaga acgcttggaa
    28681 gttgtcaaag caatctatga aactggtgtt cgtattcctt ctatctgttt ttcaggccat
    28741 cgtcgctacc cattgggttc aaaagatcca gttctagagg aaaaatctct agaactcatg
    28801 aaaaaatgta tcgaattagc tcaagacttg ggagttcgta cgattcaatt agctggttac
    28861 gatgtttact atgaggaaaa gtcaccccag acacgccaac gttttatcaa aaatttgaga
    28921 aaagcctgtg actgggctga agaagctcag gtggtacttg ctattgaaat tatggatgat
    28981 cctttcatca atagcatcga aaaatatttg gctatagaaa aagagattga ctctcccttc
    29041 ctctttgtat atccagatat tggtaatgtg tctgcatggc ataatgatat ctatagtgag
    29101 ttttatcttg gtcatcatgc catcgcagct ctccatctca aggatactta tgcagtgaca
    29161 gaaagttcaa agggccagtt ccgagatgta cctttcgggc aaggttgtgt caaatgggaa
    29221 gaagctttcg atattttaaa ggaaaccaat tataatggac ctttcctaat cgaaatgtgg
    29281 tctgaaaatt gtgaaacagt agaagaaaca cgcgcagccg ttcaagaggc gcaagctttt
    29341 ctctatccac tcattaagaa agcaggtttg atgtaagatg aatcaagtaa tcaatgctat
    29401 gcgtaaacga gtctgtgatg ccaatcaatc attgccaaaa catggacttg tcaaatttac
    29461 ctgggggaat gtatctgaag ttaatcgcga actcggtgtc attgttatca aaccatcagg
    29521 cgtggattat gacgaattga cacctgaaaa catggtagtg actgatctag atggtaagat
    29581 cctagaaggg gatttaagac catcttccga cctcccaact catgtgcaat tatataaggc
    29641 ttggtcagaa attggtagtg tggttcacac ccattcgaca gaagctgttg cttgggctca
    29701 ggcaggtcgt gatattcctt tctacggaac aacccatgca gattatttct acggttcaat
    29761 cccttgcgcc cgtagtttga ccaaggacga agtagaagtg gcctatgaaa aagatactgg
    29821 cctggttatc gtagaagagt ttgaacatcg cggacttaac ccggttgaag taccaggaat
    29881 tgttgtacgc aatcacggtc cattcacctg gggcaaaaat ccagagaatg ctgtttatca
    29941 ctctgtcgta ctagaggaag tatcaaagat gaatcgcttt acagaacaaa tcaatccaag
    30001 agttggacct gctccccagt acatactaga aaaacactac caacgtaaac atggaccaaa
    30061 tgcttattat ggtcaaaagt aagaacgatg aaggaggaga aaaagataaa tttagctcct
    30121 ctttttacat ttgattttta ttgagagtaa agttggaatt gaagtaattt taaaagattt
    30181 tttagaaata gcgcttgata tatatatggt aaaataaaaa gaattgctgt gatatcaata
    30241 gatttggggg attttttaat atggtactgg ataaggcaag ttgtgatttg cttcaatatt
    30301 tgatggatca agaaacgtcc aaaacgatta tggcgatttc gaaagatttg aaagagtcaa
    30361 gaaggaaaat ttattatcac attgacaaaa tcaatgctgc tctgggtgac gaggcgcttc
    30421 acatcattag tattccacga attggtattc acttaacgga agagcagaga gatgcttgtt
    30481 gtaaactatt atcggaagta gattcgtacg attatatcat gagtgcgcat gaacgtatga
    30541 tgataatgtt actatggata ggtatttcta aagaacgtat tacgattgaa aaattgatag
    30601 agttaacaga ggtatctagg aatactgttc tcaatgattt gaatagtatt cgttatcaac
    30661 taactttgga acaatatcag gtgatcttgc aagtgagcaa gtcacaggga taccaccttc
    30721 atgcccaccc tcttaataaa attcagtatc ttcaatcgct tctatatcat atttttatgg
    30781 aagaaaatgc cacttttgta tctattttag aagataagat gaaagagagg ttagatgatg
    30841 agtgtttgct ttctgttgaa atgaaccaat tttttaagga acaggttcct ttagttgaac
    30901 aagatttagg gaagaaaata aaccatcatg aaataacttt tatgttgcag gttctacctt
    30961 atttgctgtt aagctgtcat aatgttgaac agtatcaaga aagacatcag gatatagaga
    31021 aagaattttc tttgataaga aaaagaatag agtatcaggt gtctaagaaa ttaggagaac
    31081 ggttgtttca aaagtttgaa atttctttgt caggacttga agtttctctt gtagctgttc
    31141 tcctcctctc ctatcgtaaa gatttggata ttcatgcaga aagtgatgat tttcggcaat
    31201 taaaacttgc tttagaagaa tttatctggt attttgaatc acaaatccga atggagattg
    31261 agaacaagga tgatttgtta cgaaatttga tgatccactg taaatccttg ttatttagaa
    31321 agacttacgg tattttttct aaaaatcctc taacaaaaca aattcgatcc aagtatggag
    31381 aattattttt agtcactaga aaatctgcgg aaattttaga aggagcatgg tttattcggc
    31441 taacagacga tgatattgcc tatttgacga ttcatattgg aggattttta aagtatacac
    31501 catcgtctca aaaaaatatg aaaaaagttt atctcgtttg tgatgaaggt gttgcggttt
    31561 cgagactttt gctgaaacaa tgcaaacttt attttccaaa tgagcaaatt gacactgtat
    31621 ttacaacaga acaatttaag agtgtggaag atattgcaca agttgatgta gtgattacta
    31681 ctaatgatga tttggatagc agatttccga ttttaagggt taatcctatc cttgaagcag
    31741 aagatatttt gaaaatgcta gactatctta aacacaatat atttcgtaat aagagcaaaa
    31801 gtttcagtga aaatctttct agtcttattt cgtcttatat tgtagacagc aagttggcta
    31861 gtaagttcta agaagaggtt caaacactta taaatcaaga aatagtagtt caagcttttt
    31921 tggaagatat ttgaaggaca gtccaatgat gaacacaaac ctgtgttttt cttggtcttt
    31981 tttagtgttt tgaagggtgg tatactaatc tcaaagataa caattatatc caaaggaggc
    32041 aacatatgcc aaacgtcaaa gaaattacaa gagagtcatg gattttagcc actttcccag
    32101 agtggggaac atggttgaac gaagaaatcg aagaagaagt cgtacctgaa ggcaactttg
    32161 ccatgtggtg gctaggcaac tgtggtactt ggattaagac accagctggt gctaacgttg
    32221 tcatggacct ttggtcaaac cgtggaaaat caaccaaaaa agtgaaagat atggttcgtg
    32281 ggcaccaaat ggcaaatatg gcaggtgttc gtaaattgca accaaacttg cgtgttcagc
    32341 caatggttat cgatccattt gctatcaacg aactagacta ttacttagtt tcacacttcc
    32401 acagtgatca tatcgaccca tacacagctg cagcaattct caacaatcct aagttagagc
    32461 atgttaagtt tatcggtcct taccactgtg gacgaatctg ggaaggatgg ggggttccaa
    32521 aagaacgtat catcgttgtt aaaccaggtg acactatcga attaaaagat atgaagattc
    32581 atgcagtaga atcatttgac cgtacttgct tggtaactct cccagtgaac ggtgctgatg
    32641 agacaggcgg tgaacttgct ggcttggctg ttacagatga agaaatggct caaaaggctg
    32701 ttaactatat ctttgaaaca ccaggtggaa ccatctatca tggtgcagat tctcacttct
    32761 caaactattt tgcaaaacat ggtaaagact ttaaaattga tgttgctttg aataactatg
    32821 gtgaaaatcc ggtaggtatc caagacaaaa tgacatctat cgaccttctt cgtatggcag
    32881 aaaatctgcg taccaaagtc attatcccag ttcactatga tatctggtct aacttcatgg
    32941 cttctactaa tgagattcta gaactttgga aaatgcgaaa agatcgcttg caatacgatt
    33001 tccatccatt tatctgggaa gttggcggta agtacactta tcctcaagat caacacttag
    33061 tagaatacca tcatccacgt ggttttgatg attgttttga acaagactct aacattcaat
    33121 ttaaagcttt gctataatat aaaaatattc tttggaggtt atccgtatgt tgcatgatac
    33181 ggataatttt catataatag taaaatagaa tgtgtgattc aataatcacc tcaaatagaa
    33241 aggaaattct atgtcaaatc tatctgttaa tgcaattcgt tttctaggta ttgacgccat
    33301 taataaagcc aactcaggtc atccaggtgt ggttatggga gcggctccga tggcttacag
    33361 cctctttaca aaacaacttc atatcaatcc agctcaacca aactggatta accgcgaccg
    33421 ctttattctt tcagcaggtc atggttcaat gctcctttat gctcttcttc acctttctgg
    33481 ttttgaagat gtcagcatgg atgagattaa gagtttccgt caatggggtt caaaaacacc
    33541 aggtcaccca gaatttggtc atacggcagg gattgatgct acgacaggtc ctctagggca
    33601 agggatttca actgctactg gttttgccca agcagaacgt ttcttggcag ccaaatataa
    33661 ccgtgaaggc tacaatatct ttgaccacta tacttacgtt atctgtggag acggagactt
    33721 gatggaaggt gtctcaagcg aggcagcttc atacgcaggt ttgcaaaaac ttgataagtt
    33781 ggttgttctt tatgattcaa atgatatcaa cttggatggt gagacaaagg attcctttac
    33841 agaaagtgtt cgtgaccgtt acaatgccta cggttggcat actgccttgg ttgaaaatgg
    33901 aacagacttg gaagccatcc atgctgctat cgaaacagca aaagcttcag gcaagccatc
    33961 tttgattgaa gtgaagacgg ttattggata cggttctcca aacaaacaag gaactaatgc
    34021 tgtacacggc gcccctcttg gagcagatga aactgcatca actcgtcaag ccctcggttg
    34081 ggactacgaa ccatttgaaa ttccagaaca agtatatgct gatttcaaag aacatgttgc
    34141 agaccgtggc gcatcagctt atcaagcttg gactaaatta gttgcagatt ataaagaagc
    34201 tcatccagaa ctggctgcag aagtagaagc catcatcgac ggacgtgatc cagtcgaagt
    34261 gactccagca gacttcccag ctttagaaaa tggtttttct caagcaactc gtaattcaag
    34321 tcaagatgcc ttgaatgttg tggcagctaa attaccaacc ttcctaggtg gatcagctga
    34381 ccttgctcac tcaaacatga cttatatcaa gacggatgga cttcaagacg ataccaatcg
    34441 cttgaatcgt aacattcagt ttggtgttcg tgaatttgca atgggaacaa tcttgaacgg
    34501 gatggccctt catggtggac ttcgtgtata cggtggaact ttcttcgtct tctctgacta
    34561 tgtgaaggca gctgtccgct tgtcagcctt acaaggactt cctgtgactt atgtctttac
    34621 ccatgattca atcgcagttg gggaagatgg tccgactcac gaaccggttg agcatttagc
    34681 aggtcttcgt gctatgccaa atctaaatgt tttccgtcca gcagatgcgc gtgaaacgca
    34741 agcagcttgg taccttgcag tgacaagtga gaaaacacca actgcccttg tcttgacacg
    34801 tcaaaacttg actgttgaag atggaacaga cttcgacaag gttgctaaag gtgcttatgt
    34861 tgtatatgaa aatgcagccg actttgatac catcttgatt gcgacaggtt cagaggttaa
    34921 tcttgctgtc tcagctgcta aagaattggc tagtcaaggc gaaaaaatcc gcgtagtcag
    34981 catgccatct acagatgtct ttgataaaca agatgcagct tacaaggaag agattcttcc
    35041 aaatgcagtc cgccgtcgtg ttgcagtcga aatgggtgca agtcaaaact ggtacaaata
    35101 tgttggtctc gatggtgccg ttctaggtat tgataccttc ggagcctctg ccccagcacc
    35161 aaaagtattg gcagaatatg gctttactgt agaaaatctt gtaaaaattg ttcgaaactt
    35221 gaaataatcc taaaaatcag ggcgtaagct ctggtttttc ttaccagaaa agtaaggtac
    35281 aatcttgtaa aagtagctaa aatttgatat agtagtccta tgtaaaagac aaaggagaaa
    35341 acaatgaatc caaatattac ttttttaatc atgcttgtag gtatgatggc cttgatgttc
    35401 tttatgcaac gttctcaaaa gaaacaagct caaaagcgta tggaaagctt aaataaacta
    35461 caaaaaggct atgaagtgat tacaatcggt ggactttatg gaacagtcga tgaagtagat
    35521 acggagaagg gaacaatagt tcttgacgta gatggagttt acttaacttt tgaactagct
    35581 gctatcaaga cagtattacc gctgaaagaa acagcttcac tagaaggcgc aattgaaaaa
    35641 taagacggga tcacgaactc ccgtttttct ataaaagaaa ggaaatggga tgaaaaaatt
    35701 agtctttgtc tgtctgggaa atatttgccg tagccctatg gccgagtttg ttatgaaatc
    35761 aatgacagat aactacgaaa tccaaagtcg agcaacttcc tcttgggaac atggcaatcc
    35821 gattcataag gggactcagg gaatttttca acagtatgag attccttatg acaagaacaa
    35881 gacatcgctt cagattagta aggaagattt tgaagccttt gattatatta tcggaatgga
    35941 cgcttcaaat atttctgact tacgtcagat gtgtccagta gactgtcaag ataagattta
    36001 ctcattttca tctgaaagtg ttccagaccc ttggtataca ggagattttg aagaaaccta
    36061 tcgacgtgtt caagagggct gtcaagcttg gttagaacgt ttagaaaagg agagttaagg
    36121 tggaaaatct tacgaatttt tacgaaaagt atcgtgttta tctgactcgt ccacgtttag
    36181 agattttggc agtagttacc attgttttct gtgctgtact cgtctttttt ctaaatattc
    36241 caggaaaagg tgtcttaaaa ctcgataatg gaacgattgt ttatgatggc agtcttgtcc
    36301 gcggtaaaat gaatggccaa ggtaccatta ccttccaaaa tggagaccaa tatacaggtg
    36361 gcttcaacaa tggagccttc aacggaaaag gtacctttca atctaaagaa ggctggacct
    36421 acgaaggtga ttttgtaaat ggtcaggctg aaggaaaagg gaaactaaca acagaacaag
    36481 aagtcgttta tgaaggaact tttaaacaag gcgtttttca acaaaaataa agcctcctta
    36541 tcaaaggagg tattattaga attacaaggt aagcgcttac ctgtaaatcc ctttctttcc
    36601 aaatccctct tccaagcaag tttgtgaaat aaaaaatatt tgaaataaat ttcacaaact
    36661 tcaaagataa aacctgataa gaaaagaaaa tgagaaaagt ttcacaagag tttaaaaatt
    36721 ctttgtgaaa tgtttatgaa actgtttaca aagttgaaat aatgcgttat aataaacttg
    36781 tgaaaaaatt aacaaaggat atcgatcctt gaaagctatg gaggaaaata tggctgataa
    36841 aaaaactgtg acaccagagg aaaagaaact cgttgctgaa aaacacgtag atgagttggt
    36901 tcaaaaagct ctagttgccc ttgaagaaat gcgtaaattg gatcaagagc aagttgacta
    36961 catcgttgcc aaagcatcag tagcagcttt ggatgcccac ggagaattgg ctttacatgc
    37021 ctttgaagaa acaggacgtg gtgtatttga agataaagca actaagaact tgtttgcctg
    37081 tgaacacgta gtaaacaaca tgcgtcacac taagacagtt ggcgttatcg aagaagacga
    37141 tgtaacagga ttgactctta ttgctgaacc agttggtgtt gtttgtggta ttactccaac
    37201 aacaaaccca acatcaacag caatcttcaa atcattgatt tcattgaaga cacgtaaccc
    37261 aatcgtcttt gccttccatc catcagcaca agaatcatct gctcatgcag ctcgtatcgt
    37321 ccgcgatgca gctatcgcag ctggtgctcc tgaaaactgt gtgcaatgga ttactcaacc
    37381 atctatggaa gcaacaagtg cccttatgaa ccacgaaggt gttgcgacaa tccttgcaac
    37441 aggtggtaat gccatggtta aggcggctta ttcatgtggt aaaccagctc ttggggtagg
    37501 tgccggaaac gttccagctt atgttgaaaa atcagcaaac attcgtcaag cagcacacga
    37561 tatcgtcatg tctaaatcat ttgataacgg tatggtctgt gcatctgaac aagcagttat
    37621 cattgataaa gaaatttacg atgaatttgt agcagagttc aaatcttacc acacttactt
    37681 tgtaaacaaa aaagaaaaag ctcttcttga agagttctgc ttcggcgtca aagcaaacag
    37741 caaaaactgt gctggtgcaa aattgaacgc tgacatcgtt ggtaaaccag caacttggat
    37801 tgcagaacaa gcaggattta cagttccaga aggaacaaac attcttgctg cagaatgtaa
    37861 agaagttggc gaaaatgagc cattgactcg tgaaaaattg tcaccagtta ttgcagtttt
    37921 gaaatctgaa agccgtgaag atggtattac taaggctcgt caaatggttg aatttaacgg
    37981 tcttggacac tcagcagcta tccacacagc tgacgaagaa ttgactaaag aatttggtaa
    38041 agctgttaaa gctattcgtg ttatctgtaa ctcaccttct acttttggtg gtatcgggga
    38101 cgtttacaat gccttcttgc catcattgac acttggatgt ggttcttacg gacgcaactc
    38161 agttggggat aacgttagtg ccattaacct cttgaatatc aaaaaagtcg gaagacggag
    38221 aaataacatg caatggatga aacttccttc aaaaacatac tttgaacgtg attcaattca
    38281 ataccttcaa aaatgtcgtg acgttgaacg tgtcatgatc gttactgacc atgccatggt
    38341 agagcttggt ttccttgatc gtatcatcga acaactggac cttcgtcgca ataaggttgt
    38401 ttaccaaatc tttgcggatg tagaaccgga tccagatatc acaactgtaa accgtggtac
    38461 tgagattatg cgtgccttca aaccagatac catcatcgca ctcggtggtg ggtctccaat
    38521 ggatgctgcc aaagtaatgt ggctcttcta cgagcaacca gaagtggact tccgtgacct
    38581 tgtccaaaaa ttcatggata tccgtaaacg tgccttcaag ttcccattgc ttggtaagaa
    38641 gactaaattc atcgcgattc caactacatc tggtacagga tctgaagtaa caccatttgc
    38701 cgttatctct gataaagcaa acaaccgtaa atacccaatc gctgactact cattgacacc
    38761 aactgtggca atcgtagatc ctgctttggt attgacagtt ccaggatttg ttgctgctga
    38821 tactggtatg gacgtattga ctcacgcaac agaagcatat gtatcacaaa tggctagtga
    38881 ctacactgat ggtttagcac ttcaagccat taaattggtc tttgaaaatc tcgaaagctc
    38941 agttaagaat gcagacttcc actcacgtga gaaaatgcat aacgcttcaa caatcgctgg
    39001 tatggccttt gccaatgcct tcttaggtat ttctcactca atggcccata agattggtgc
    39061 gcaattccac acaatccacg gtcgtacaaa tgctatcttg cttccgtacg ttatccgtta
    39121 caacggtaca cgtccagcta agacagcaac atggcctaag tacaactact accgtgcaga
    39181 tgaaaaatac caagatatcg cacgcatgct tggacttcca gcttctactc cagaagaagg
    39241 ggttgaatct tacgcaaaag ctgtctacga actcggtgaa cgtattggga tccaaatgaa
    39301 ttttagagac caaggaattg acgaaaaaga atggaaagaa cattctcgtg aattagcctt
    39361 cctggcttat gaagaccaat gttcaccagc taacccacgt cttccaatgg tagaccatat
    39421 gcaagaaatc atcgaagatg catactatgg ctacaaagaa agaccaggac gccgtaaata
    39481 attgtttatc agtctagaag caagacaaaa actcaatttg agggaaagat ccagtaattt
    39541 ttctatgata aaaggcatcc tatcaaggtt tttgaatacc tgataggatg cctttttatg
    39601 atgttgaggc ctttttgccc tttttgaaaa actagaatag aaacaaaata tatagcagat
    39661 tgaaactaga atagtacaca tctgcttcta aaacatttat agaaattaat tttactttcc
    39721 tgatagagtt gtttacatct tatttcaatt cactatagtt taatttaaga gtagtattta
    39781 ctaaggccca attaaaatca aagagcaaac tagaaaacga gtgccattca gctcaaaaca
    39841 ctgatttgag attgcagata agactagccc cctcattaac agatttacga taaaacgatg
    39901 acaaggtgtg ttgctttttg atttctaaag agtataatga tagatctcta taaaataagt
    39961 gcgaaggaaa tgagctttta tagtcctttc gttttaaaat actatctcag atattcttat
    40021 atcgac
//
LOCUS       DAVLYV010000012        34951 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLYV010000012 DAVLYV010000000
VERSION     DAVLYV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34951
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.012"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            640..2655
                     /locus_tag="VYO70_000625"
     CDS             640..2655
                     /locus_tag="VYO70_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1712898.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDY
                     SPNGNVLIVTPHYLERQNIPVDTTIKQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            2657..3289
                     /locus_tag="VYO70_000626"
     CDS             2657..3289
                     /locus_tag="VYO70_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712899.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            complement(4217..4630)
                     /gene="ndk"
                     /locus_tag="VYO70_000627"
     CDS             complement(4217..4630)
                     /gene="ndk"
                     /locus_tag="VYO70_000627"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1712900.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEESLPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(4741..8418)
                     /gene="rpoC"
                     /locus_tag="VYO70_000628"
     CDS             complement(4741..8418)
                     /gene="rpoC"
                     /locus_tag="VYO70_000628"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1712901.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYIHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLLYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKTEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(8451..12062)
                     /gene="rpoB"
                     /locus_tag="VYO70_000629"
     CDS             complement(8451..12062)
                     /gene="rpoB"
                     /locus_tag="VYO70_000629"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1712902.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            12478..12548
                     /locus_tag="VYO70_000630"
     tRNA            12478..12548
                     /locus_tag="VYO70_000630"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12510..12512,aa:Cys,seq:gca)
     gene            complement(12746..14077)
                     /locus_tag="VYO70_000631"
     CDS             complement(12746..14077)
                     /locus_tag="VYO70_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1712903.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(14368..15192)
                     /gene="endA"
                     /locus_tag="VYO70_000632"
     CDS             complement(14368..15192)
                     /gene="endA"
                     /locus_tag="VYO70_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1712904.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(15231..15419)
                     /locus_tag="VYO70_000633"
     CDS             complement(15231..15419)
                     /locus_tag="VYO70_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1712905.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(15412..16695)
                     /gene="murA"
                     /locus_tag="VYO70_000634"
     CDS             complement(15412..16695)
                     /gene="murA"
                     /locus_tag="VYO70_000634"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1712906.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKVSQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(16719..16949)
                     /locus_tag="VYO70_000635"
     CDS             complement(16719..16949)
                     /locus_tag="VYO70_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1712907.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(17007..18044)
                     /locus_tag="VYO70_000636"
     CDS             complement(17007..18044)
                     /locus_tag="VYO70_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1712908.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKEIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(18028..18516)
                     /gene="coaD"
                     /locus_tag="VYO70_000637"
     CDS             complement(18028..18516)
                     /gene="coaD"
                     /locus_tag="VYO70_000637"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1712909.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            complement(18506..19045)
                     /gene="rsmD"
                     /locus_tag="VYO70_000638"
     CDS             complement(18506..19045)
                     /gene="rsmD"
                     /locus_tag="VYO70_000638"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1712910.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(19110..20102)
                     /locus_tag="VYO70_000639"
     CDS             complement(19110..20102)
                     /locus_tag="VYO70_000639"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1712911.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(20214..20897)
                     /locus_tag="VYO70_000640"
     CDS             complement(20214..20897)
                     /locus_tag="VYO70_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1712912.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(20953..21693)
                     /locus_tag="VYO70_000641"
     CDS             complement(20953..21693)
                     /locus_tag="VYO70_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1712913.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFYLS"
     gene            21727..22005
                     /locus_tag="VYO70_000642"
     CDS             21727..22005
                     /locus_tag="VYO70_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1712914.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYYDFKIAN"
     gene            22083..23009
                     /gene="yidC"
                     /locus_tag="VYO70_000643"
     CDS             22083..23009
                     /gene="yidC"
                     /locus_tag="VYO70_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1712915.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(23191..23985)
                     /gene="pflA"
                     /locus_tag="VYO70_000644"
     CDS             complement(23191..23985)
                     /gene="pflA"
                     /locus_tag="VYO70_000644"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1712916.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(24111..25361)
                     /locus_tag="VYO70_000645"
     CDS             complement(24111..25361)
                     /locus_tag="VYO70_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1712917.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTPAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(25428..26255)
                     /gene="purR"
                     /locus_tag="VYO70_000646"
     CDS             complement(25428..26255)
                     /gene="purR"
                     /locus_tag="VYO70_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1712918.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(26522..27463)
                     /locus_tag="VYO70_000647"
     CDS             complement(26522..27463)
                     /locus_tag="VYO70_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1712919.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(27453..28709)
                     /locus_tag="VYO70_000648"
     CDS             complement(27453..28709)
                     /locus_tag="VYO70_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1712920.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(28711..29373)
                     /locus_tag="VYO70_000649"
     CDS             complement(28711..29373)
                     /locus_tag="VYO70_000649"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1712921.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFEQNPQAEV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMRQIEIEDGQNLIAYCSEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(29336..29992)
                     /locus_tag="VYO70_000650"
     CDS             complement(29336..29992)
                     /locus_tag="VYO70_000650"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1712922.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(30003..30881)
                     /gene="rsgA"
                     /locus_tag="VYO70_000651"
     CDS             complement(30003..30881)
                     /gene="rsgA"
                     /locus_tag="VYO70_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1712923.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(30883..31755)
                     /gene="rsmA"
                     /locus_tag="VYO70_000652"
     CDS             complement(30883..31755)
                     /gene="rsmA"
                     /locus_tag="VYO70_000652"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1712924.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(31886..32194)
                     /locus_tag="VYO70_000653"
     CDS             complement(31886..32194)
                     /locus_tag="VYO70_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712925.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQTVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(32187..32828)
                     /locus_tag="VYO70_000654"
     CDS             complement(32187..32828)
                     /locus_tag="VYO70_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1712926.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(32830..34860)
                     /locus_tag="VYO70_000655"
     CDS             complement(32830..34860)
                     /locus_tag="VYO70_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1712927.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFIDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
BASE COUNT         9954 a         7950 c         6879 g        10168 t
ORIGIN      
        1 aaagaaaagg gtttcacgaa tgaagaaaaa tcgtggaatt caaaaattag ctatattagt
       61 attactaggt gtttttatgt ttagtaacac aattccttac caacagttta ttcagaagaa
      121 tagacaattg gagattcgag tgcaatcaca aaagaagtcc aatggtcttg atgttgtgaa
      181 ggctgattga tttcgtatta ctttaaaatt caactattta gagaaaggta ttctgatgag
      241 agcttgtaac aataccagat agatataaga atggagatgt ttgataaaat gctttcatag
      301 ctaaccaact gttgaaaatt atgagttaca aaatctaaag tatttaagat tggtttataa
      361 aattaggtct ctgcctgtag aaaggaacgt gcagttagaa aggaggaaaa aataggatac
      421 tataattcag actgagattt ctaattaaaa tatgtagttg catttcgctc attttcataa
      481 tatttcattc agaggagtaa taatatgtta aaatttagat ttgatgcggg atggtaaaga
      541 ataattgaag aagattcttt tatcaatagt ttagtaagct tgttagttta tagagggcag
      601 gggtatttct gccctctgtt ttagaataag gaatgcacta tgaaaaaatt atttatactc
      661 atatccaacc tgcttgctag tttgttcttt gtttgggtat ttaccatttg gactgataca
      721 tatgtctcct attattaccc caacgttgtt gtacatgatt cttcccctga aacaactttt
      781 cagcatgttg ctacacgctt ggaaaaacta gcagaagaaa cagatagctt tattgcgatt
      841 caacaccaag atcctaactc agaaggtact acagtttttt cttatacgac ctttggggac
      901 ggaaagttgc ctgatgggct tcaggaaaaa aatctagaag atgctcaaag tagtagtgtt
      961 gaaacaaact attttgtatt cgatggacac ctagatattc acttgctaag ggaagagcta
     1021 agtcaacttg gcttgactaa catgcacctg acaattccat ctaaactatc tacgttaatg
     1081 gctatcttta gtaacggatt tcagttaatc agtctattga ttttcatctt aacctttgta
     1141 gcactgactt taattagtca aattagccaa ttacggtcat cgggcattcg cctcatttca
     1201 ggagagaaac ggtggtccat tttccttaga ccagttggtg aggatttgaa agggatagct
     1261 gttggtttca gtttagctgg cgtgctcgcc attcttatgc aaaaaattct gtcgcttcca
     1321 acgcaatccc taatgaccat aggagcaggc ttgctcagtt ataatctcat cttgttgtcg
     1381 atctccctgt ttttcgctca actcttcgca gttgggataa aaaagataca cctgatgcag
     1441 attataaaag ggcaagtgcc tgtcagagga atcattagtt tgattttaat cggtcaactg
     1501 ttagcgatta ttattgtaac gctgggaata gggagtagct taaagtattc ccaagcctgg
     1561 caacagcatc ggattggaca agaggtttgg agtcaggaaa gacaactgat taccctatca
     1621 atcagccgtg aaggaacgag tcctggtttt gatgaacaag ctcaaaggaa actcagaact
     1681 tggtatcaat tgatggatct ggctgtttca gaacaaaagg ctttcttatc tagacaccag
     1741 ttaattgacc gtactttgca aaatggcatg gcttcctcca aaaacttgac aacctctaca
     1801 gaatggcacg attacagccc gaatggcaat gtccttatcg tcacaccgca ttacttggag
     1861 cgtcaaaaca ttcctgtaga tacaactatt aagcaaaaga tgaatcacct taatgtaggg
     1921 gagtttgtct tattgctgcc tgaacacctc cgttcagagg aagaacatta taaatctgtt
     1981 tttgaagacg acttaaccag tcgcatgtct agtcaagatg aacgacagca aatgactgct
     2041 acggtaggtt atttagaatc aggtcaggat cgttttgtgt ataatacgac ccctatttct
     2101 taccagcagt ttttgaaaga tccaatcatc attgttataa caccccaatc aactggtcca
     2161 cagtccattt tgttttggat agacgcagta cagaactacg ttctctttaa tcaattgtct
     2221 gatgcccagg agcttatcca gagacaaggc attgaaaatt gggtctcaga aatgcaaaca
     2281 ggttaccaca actacatcac attattggat aatatccaga gggaacgttg ggtaatgcta
     2341 gcaggagctg tgcttgggat tgcaacttca atcttgttgt ttaacactat gaataggctc
     2401 tactttgaag aatttagacg tgccattttt atcaaacgca ttgcaggtct caggttctta
     2461 gaaatccatc gcacttatct ctttgctcaa ctgggtgtgt ttttactggg atttgttgcg
     2521 agtgtatttc ttcaggtaga gataggagtt gctttcttag tcttgttact ctttactggt
     2581 ctatctcttt tacagttaca tgtccaaatg cagaaagaaa acaagatgtc cattcttgtt
     2641 ttgaagggag gttaatatga ttgaacttaa acaggtgagt aaatcttttg gagaacgaga
     2701 gttattttcg aatctttcaa tgacatttga ggctggaaaa gtctatgcct taattggttc
     2761 aagtggtagc ggaaaaacaa ccttgatgaa catgattggg aaattagaac cttatgatgg
     2821 gacgattttt taccgaggta aagacttggc caattataaa tcgagtgatt ttttccgtca
     2881 cgaattgggc tacctcttcc agaactttgg cttaattgaa aaccaaagta ttgaagaaaa
     2941 ccttaagcta ggtctcattg gtcaaaagtt gagtcggtcg gaacagcggt tgaggcagaa
     3001 gcaggcttta gaacaggtcg gcctggctta tcttgaccta gataagcgca tctttgagtt
     3061 atcgggcgga gaatcgcaac gggttgcctt ggcaaaaatt atcttaaaga atccaccctt
     3121 tattctggca gatgagccaa cagcttcaat agacccagca acctctcagt tgattatgga
     3181 gattttgcta tctcttcgag atgataatag gctaatcatt atcgcaacac ataatccggc
     3241 aatttgggag atggctgatg aagtgttcac gatggatcat ctgaaataaa aatccttgtt
     3301 tttaattgca cgatgagtta ctgaaatatt atcatgaatc aataattgga gttaatttag
     3361 aattgtactt tattaatatt gaggtaactt tttcttgata aaggaagaaa taatggagag
     3421 gaagttagaa tgaaaaaatt cgacaattat attattgaga agccttgcga ttctaattca
     3481 gataaactgc aaaaaatctt aataattgaa agtttggtag atgatatttt gcaattttct
     3541 ctcagaatca ataatagtgt aggagagatt ttcctcctac aaccgtttta aaagaaaact
     3601 atctttattc catgttattt tgaggaagat attgtgaaag tcaaagatga tgataaagtt
     3661 gagtggaatt tgttagaatt tcaaaaattt agagcatttt tggcttagta atctgtgttg
     3721 aaggctcaaa acctatggta aaaaagtcgc tttgaaaacg tattgcctcc aaagatttag
     3781 ttaaataatg atttaacaca aaaagaaatt attgaagttc tggaaagatg ttgtttcagt
     3841 attgagaaaa ggtgggaaaa acttgcgatt ttcacagaga aaggaagaaa aagtatagaa
     3901 atatagtcaa ttgaaacaag aacaggacaa aagaaccttt tgtgccatat ttttctcctt
     3961 tcgctttaca attggcttga acacctttat tgtatcgcgt ttggagtttt tttggtataa
     4021 ccttcgacgc gcacccgcat agcgggtgtt tttttgtctc gcacctaacg gagcgagacg
     4081 aactaatagt cacttaatca aaaaacgcac catatcaaaa actaaaaagt ttgatatcat
     4141 gcgtcatgtc ttaaactaat tgactatact ttctattcaa atgagctttt aaccaattga
     4201 ttgagccaat ccactcttaa aaccaaagag caatttctcg cttagctgac tcttctgaat
     4261 ctgaaccatg tacaacattt tggataacct cattttctcc agcagctttt gcaaagtcac
     4321 ctcgaatagt gcctggtaaa gattcttctg gacgagttgc acccatcatg gtccgccaag
     4381 tttcgattac tttgggacca gaaatgacac ccacaagaac tggacctgaa gtcatgaatt
     4441 cacgaatcgg taggtaaaaa ctctgaccaa ccaagtcctg atagtgctgg tcaatcaact
     4501 cttctgaaac ctgtgaacga aactccaatt tttcgattgt aaatccacgt tgttcgatgc
     4561 gctttaacac ttcacccact agccctcttt ttacaccatc tggtttgatg ataaagaatg
     4621 tttgttccat acccgtctcc tttgtcagct tctttctttt attttaccac atttcgtgga
     4681 aaaatggaga aagttttcag aagagagaat gagagaaccc gagggttctc tcattctctc
     4741 ttattctact gtttcttcca cagtttcaac ggcagtatcc acaactactt ctgttgtttc
     4801 ttcatttcct tcttcctcta ctggaggatt aaggtattct tcttcgttga cagcatgtgg
     4861 ttcaaggtta cggtaacggg ccataccagt accagctggg atgatcttac cgatgataac
     4921 attttcttta agtccaagga gatggtcttt cttaccacgg atagctgcgt cagtaaggac
     4981 acgagttgtt tcttggaagg aagctgctga caagaaactg tttgtttcaa gtgaggcttt
     5041 ggtaattccc ataaggactg ggcgacctgt cgctggaact ccacctgcga taaggacatc
     5101 tttgttggca tctgtaaagt cattgatatc catgagggta cccatgagaa gatctgtatc
     5161 acctggatcc atgacacgga ctttacggat catttgacga accattacct cgatgtgttt
     5221 gtcaccgatt tctacccctt ggctacggta aactttttgt acttcaccga gaaggtacgt
     5281 ttcaactgac aagacatcac gaactgcaag gagacgtttt ggttggatag aaccttctgt
     5341 cagagcagca ccacgcgcta cttggccccc aacttcgaca cgcatacgag ctgtaaatgg
     5401 aacgacatat tcaccttcgc cagtttcacc cttaacaaag actttcttgg tacgagttga
     5461 tgcatcttct tcgatagcag taacttgtcc tttaacctct gtgataaccg cttccccttt
     5521 aggattacgg gcttcaaaga tttcttggac acgaggaaga ccctgagtga tatcggtatt
     5581 tgaggcaacc ccacctgtgt ggaaggtacg cattgtaagc tgtgtaccag gttccccgat
     5641 agattgggca gcaattgtac caactgcttc accaacttca accgcatcac cagtcgccaa
     5701 gttgataccg taacagtgac ggcagacacc gtgacgagtg ttacatgtaa atacagaacg
     5761 gatagtcact tcttccacac cagcattgac aatttcacgc gccttgtctt ctgtaatcaa
     5821 ttcatttgga ccaataatca ctgcaccagt ttctggatgt ttaacagttt tcttagtgta
     5881 acgaccgttg agacgctctt cgagagactc gatcatctct tttccttctg cgatagaacg
     5941 gatcaagaga ccacggtcag ttccacagtc gtcctcacgg atgataacgt cttgggcaac
     6001 gtcgaccaaa cgacgagtca agtaacctga gtcggctgtc ttaagggccg tatcggtcat
     6061 acctttacga gcaccgtgag ttgagaagaa catttccaat accgacaaac cttcgcggaa
     6121 gtttgaaagg attggcaatt ccatgatacg tccattcgga gcagccatca gaccacgcat
     6181 accggcaagc tgtgagaagt ttgagatgtt accacgggct ccagagtcca tcatcataac
     6241 gattgggttc ttaggatctt ggttagcaat caagcgtttc tcaagttttt cacgggcagc
     6301 acgccattca gctgtaacag cattgtaacg ctcgtcgtct gtgatcatac cacgacggaa
     6361 ttgtttggtg atttgttcga cacgtttgtg tgattcttca atgatttcag tcttgtcatc
     6421 aacgactggg atatcggcaa tacccactgt caatcctgca agagttgagt ggtggtaacc
     6481 gaggttcttc atgcggtcaa gtagggcaga agtttctgtc gtacggaaac gtttgaagat
     6541 ttcagcgatg atatttccaa ggtttttctt cttgaatgga gggttgagct caagattgct
     6601 gatagcttcc ttgatatctc caccaagtgg caagaagtat ttagctggaa caccttctgt
     6661 caagttggca ttgtttggtt cttgcaagta tagtagcccc tctggcatga tatcgttgaa
     6721 gagaatttta ccaactgttg taagcaagac cttatgtctt tgctcttctg tccaaggctt
     6781 gttgaggctg tctgttgcga taccaacacg tgagtggagg tgaatataac cattgcggta
     6841 agccataacc gcttcgtcac ggtctttgaa gaccattcct tcaccttcgc gaccagcttc
     6901 ttccatggtc aagtagtagt tacccaaaac catgtcctga gatggagtaa ctaccggttt
     6961 cccatctttc gggttcaaga tgtgctcagc agctagcatg aggatacgag cttctgcttg
     7021 tgcttcttct gaaagtggta cgtggatggc catttggtcc ccgtcaaagt cagcattgta
     7081 ggcttcacag acaagtgggt gcaagcgaag agccttacca tcaatcaaga ctggctcgaa
     7141 ggcttggata cccaaacggt gaagggtcgg tgcgcggttc aaaagcactg ggtgttcttt
     7201 aatcacttct tcaaggatat cccagatacg ctcatctccg cgttccacca agcgtttagc
     7261 tgctttgacg ttttgcacga tatcacgggc aacgatttca cgcatgacaa atggtttaaa
     7321 gagttcaatc gccatttcac gcggcacacc acattggtac atcttaagag ttggaccaac
     7381 ggcgataacg gaacgtcctg agaagtcaac acgtttaccg agcaagtttt gacggaagcg
     7441 tccctgttta cctttaagca tgtggctcaa tgatttcaat ggacggctac ctggtcctgt
     7501 gattggacga ccacgacgac cattgtcaat caaagcgtca actgcttctt gaagcatacg
     7561 cttctcattt tgaacgatga tacctggggc atttaactca agcaaacgag ccaaacggtt
     7621 gttacggttg ataacacggc ggtaaaggtc attcaagtca gatgaggcaa aacggccacc
     7681 atccaactgc aacattggac gaagatctgg tgggataacc ggaaggatgt taagaatcat
     7741 ccattcaggt ttgtttccag acttgtaaaa ggcatccaaa acatccaaac gacggatggc
     7801 tttgacacgt ttttgtccag tagctgtttt caattcttct ttgagttcag caatttcttt
     7861 ttcaagatct acttgcttca aaaggtcttg gatggcttcc gcacccatct tggcaacaaa
     7921 tgaaccataa ccatattcac gcaagcgctc tcggtattcg cgctctgtca tgatagactt
     7981 gtgctcaagt ggtgtatcct taggatcaat caccacataa gccgcaaagt agataacttc
     8041 ctcgagggca cgagggctca tatcaagggt caagcccata cggcttggaa tccccttgaa
     8101 gtaccagatg tgagatacag gagctttcaa ctcgatatgt cccatacgct cacgacgaac
     8161 tttcgtacgc gttacttcaa ccccacagcg gtcacaaaca attcctctgt aacgaatgcg
     8221 tttgtactta ccacaagcac attcccagtc ttttgtagga ccaaagatca cttcatcaaa
     8281 gagtccttca cgttctggtt tcaaggtacg gtaattgatt gtttcaggtt ttttgacttc
     8341 tccataagac catgaacgga ctttacttgg agaagctagg gtgatttgca tacttttaaa
     8401 acgatttaca tcaaccacta tttcttccct ttctattcta agtgaactgc ttattcctgt
     8461 tcagcagctt cttctgttgc ttccgctttt gttgctttct cagcttcttc agcttcaaag
     8521 gctgctttag cctcttgggc tgctttttcg cgggcttttt caaggtcatc tacgtggatg
     8581 acatcttcgt ccattccttc atccaagtcg cgaagttcca cttcttggtc atcttcgtct
     8641 agaacacgca tgtcaagacc aagagattgc aattctttga caagaactcg gaaggattct
     8701 ggaacacctg gttttggaat tggtttgcct tttgtaatag cttcataggc tttcaaacgt
     8761 ccgttgatat cgtccgactt gtaagtcaag atttcttgaa ggacatttga cgcaccgtag
     8821 gcttcaagag cccaaacctc catctcaccg aaacgttgtc caccaaactg agctttacct
     8881 ccgagtggtt gttgggtaac agttgagtaa ggtccgactg aacgcgcgtg caatttatca
     8941 tcaaccatgt ggtggagttt gatcatgtac atgactccaa cagaaacacg gttatcaaat
     9001 ggttcacctg tacgtccatc gtaaaggatt gtcttggcat cgctatccat acctgcttct
     9061 ttaacagttg accaaagatc ttcagaactt gctccatcaa agactggtgt cgcaatgtga
     9121 ataccaagag tacgagctgc cataccaagg tgaagctcca taacctgacc gatattcata
     9181 cgtgatggca ccccaagtgg gttcaacatg atgtcgactg gagttccgtc tggaaggtaa
     9241 ggcatgtctt ctacaggaac gatacgagag acaacccctt tgtttccgtg acgtccggcc
     9301 attttatctc cgaccttaat cttacgtttt tgagcgatgt aaacacgaac caacatgtta
     9361 acacctgatt gcaactcatc tccatttaca cgtgtaaaga tcttaacatc acgaacgaca
     9421 ccatcggcac cgtgtggtac acgaagagaa gtatcacgca cttcacgaga cttgtctcca
     9481 aagatagcgt gcaagagacg ttcttcagct gaaagatctt tctcaccctt aggtgttact
     9541 ttacctacaa gaatatcacc ttctttaacc tcagcaccaa tacggataat ccccatttcg
     9601 tcaaggtctt tgagggcatc ttcaccaacg tttggaattt cgcgagtgat ttcttcaggc
     9661 ccaagctttg tatcgcgcgt ttctgattcg tattcttcaa ggtgaacaga tgtgtagaca
     9721 tcgtccttca ccaagcgttc gctcatgata acggcatcct cgaagttgta accttcccaa
     9781 gtcatgtagg caacgattgg gttttgtcca agcgccattt ctccattttc catagaaggt
     9841 ccgtcagcga tgaaatcgcc tttttcaacg acatcaccaa cttttacgag agtgcgttgg
     9901 ttgtaagcag tacctgagtt tgaacgacgg aatttttgga tgtggtaaac atccaatgaa
     9961 ccatcttcac gacgaacttc taccttgtca gcatctgcgt aagtaacttt accatcatac
    10021 tgagcaatca cagccgcacc agaatcgtgg gctgcttggt attccatacc agtaccaacg
    10081 taaggtgcct gaggattaat caatggcaca gcctgacgtt gcatattggc tcccatgagg
    10141 gcacggttgg agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactacc
    10201 tgttttggtg aaacgtccat gtagtcaaca atattagctg gatactcttg gttgacccct
    10261 tggtgacgtc ccatgacaat cttctcagca aaggttccat cttcattcag acgagagtta
    10321 gcctgagcta cagtatattc atcttcttca tcagctgtca accaaacaat ttcgttcgtg
    10381 acaacacctg tttcacggtc aaccttacgg tatggtgttt gaacaaaacc atatttgttc
    10441 aagtgtccgt aagatgacaa gttattgatc aaaccgatgt taggtccttc aggtgtctcg
    10501 attggacaca tacgaccata gtgagtgtag tgcacgtcac gcacttcata tccggcacgg
    10561 tcacgagtca aaccaccagg tcctaaggct gacaaacggc gtttgtgaga caactcagaa
    10621 agcgggttgt gttggtccat gaactgtgac aactgtgatg aaccaaagaa ttctttaact
    10681 gcagctgtta caggacgaat attgataatt tgttgtggtg tcaagacttc attgtcctga
    10741 acagacatac gttcacggac attacgttcc atacgagaaa gtcccaaacg tacttggttg
    10801 gcaagcaatt caccaaccgc acggatacga cgatttccaa ggtggtcgat atcatctaca
    10861 cggccaagtc cttcagccaa gttgaggaag tagctcatct cagcaaggat atctgcagga
    10921 gtcaccgtac gaaccttgtc atctgggtta gcattaccaa tgatcgttac gacgcgatct
    10981 ggatcagttg gagcaataac cttgaatttt tgaagaacaa caggctcagt cacaacggct
    11041 gcatcgtttg ggatgtagac aatcttgttc aagtcgccat ccaaatggct ttcaatgctt
    11101 tcaatcacgc tacgagtcat aatcgtacca gcttctacca agatttctcc agtttcaggg
    11161 tctaccaatg gctctgcaat ggtttggttg agcaaacgtg ttttaacatt gagtttttta
    11221 ttgattttgt aacgaccaac tgctgccaag tcataacgac gtgggtcaaa gaagcgagct
    11281 acaagcaagc tacgtgagct ttcagccgtc ttaggctcac ctggacgaag gcgttcgtaa
    11341 atttctttca aggcttcgtc tgtacgagag tccattggat tcttgtggat atctttttca
    11401 acagtgttgc gaaccaattc gctgtctcca aagatatcaa agatttcatc atcacctgag
    11461 aaaccaagag cacgaaccaa ggttgtaaat ggaatcttac gagtacggtc gatacgagtg
    11521 taggtgatat cttttgagtc gctttcaagt tccaaccaag ctccacggtt agggataaca
    11581 gttgaaccat agcccacctt accatttttg tctactttgt cgttaaagta aacacctggt
    11641 gagcggacca actgagaaac gataatacgt tcaccaccat tgatgatgaa agtacccatt
    11701 tctgtcatga ttgggaaatc accaaagaaa acttcttggg tcttgatttc gcctgtttct
    11761 ttattgatca aacggaaggt tacaaaaatt ggtgctgagt agctagcatc gtggatacga
    11821 gcttcttcta gcgtgtattt tggttccttg atttcatatc caacaaattc caactccatt
    11881 gtgtctgtga agtttgaaat tggcaataca tcttcaaaca cttccttaag accgtggtct
    11941 aggaaagctt tgaatgagtc agtttgaatt tcaatcaaat ttggtaagtc aagaacttct
    12001 ttgattcttg aaaaactacg acgggtacga tgtttcccgt attgaacgtc atgtcctgcc
    12061 aagatgattc tcctttgtaa ataagttcca agccttgtca atcaggcttt tctaatcgtc
    12121 atatggttgt aaacccctta tcaccgtgtc ctcttgacga attttcagaa tctttaagcc
    12181 tctgttacaa atgctcataa tcttgaaaaa aagcacaaaa agagcagcta aatctgactt
    12241 tttcagaaga tttaactgct gtgagccttg tctggacaat atttcagaca aaacctacga
    12301 caaatgatta cccatattat accctattta gctagatttt tcaaggggtt tcagtaggtt
    12361 tttggtaaat tttttcccat agaaaacttg gcatcacatt cgaatcacgc tatggtacaa
    12421 aaaactgaaa aaactattga ctgaaaatca ttttcaaggt ataataataa acgttaaggc
    12481 ggtatagcca agtggtaagg cacggctctg caaaagcttg atcgtcggtt caaatccgtc
    12541 taccgccttc tataacttga tttatcaggt ttcaaatgaa cagaaagccc aatttgaagg
    12601 gcttttttta ttttccctcg aataaatacg tataacttta aaaacttttg gagcgagttt
    12661 gtggcagagt tctttccatg gcataattcc cttttgaaat caattgaaaa aagcttcgtc
    12721 acgcctggtg acaaaagcct ctagtttatt ctgtttcttc ttctatttcg acttctgtga
    12781 tttggacttt taccttggta acacgtccat tcttcacttt atcattggtt aagataaact
    12841 gtttgttttg gctgaccaat tcatagctga gtttctcagt cgttggaatg gttcccactc
    12901 ctgtcaaata ataaccagcg atggtatcaa cgtcatcact ttccagctca acatcaaagt
    12961 aattattgaa atcattgagg gtcatagttc cttgaacaat gtaggtatcc tcgccaatct
    13021 gatgaacatc tattgctgct ttgtccgttt catcatcaat ttcaccaaca atttcctcta
    13081 agaggtcttc caaagtcact aaaccagcca taccaccgta ttcatccagc aagattgcca
    13141 tttgtctttg ggtatttcgc agttctttta gcaagtcatc cacaaaaata gtttcaggta
    13201 caaaaagtgg atcttgtaaa attctcttcc aaacaatatt gtcaaaaccg tccacaaagc
    13261 ctgccttaag gagactcttg gtgtgaatga ttccaattac attgtcctta tccccatcat
    13321 aaaccgggat acgagaataa ttttgtttta aaatactttg gataatggct tgactatcat
    13381 cctgaatatc caccataaag gcatctgttc gaggaaccat aacctctcgt gccatcagtt
    13441 catcgagcga aaagacacct tgtagcatct caatctcatc agcatccaat gtttcttcac
    13501 tatttgtcag catataggca atttcatcac gggtcatttt ttcatccgca tcatcgaatg
    13561 acataggagt caaatggctc aagaaattgg tcgaagcagc taaaagccaa acaaaaggac
    13621 tgactagttt tccgatccca atgataaccg gcgctgtacg aattgccaag gcatccttta
    13681 gattaagagc gattctctta ggatataatt ccccaaaaac gatggaaata taggtcaaaa
    13741 atgccaagga tagaaaagtt gccacggctt gtgctgtttc gccattccca agccaagagg
    13801 caatcacacg tcctagagta tcagttaaac tcgcccctga taagattgta atcagggtga
    13861 ttcctacctg aatggttgat aaaaagtggt taggattttc tagtaccttc agcaggcgga
    13921 tgtagcgtct gtctccttct tccgcctttt gttcaactcg ggcacgatta agagaaacca
    13981 tggccatttc tgtggctgag aaaaaggcat ttaataccgt caagataaac aataaaacaa
    14041 attgtagcaa caaattctga ctacttgggt cttccatagt tcttctccta aaatagtatc
    14101 ttatccctta ttatatcaca aaatttaatc caatacatat tatttttttc ttagcgtaac
    14161 ttccatctct ctcccaaaac tggaagttag gctaagacgg atttccactg atttcacgcg
    14221 tttttctcat actaaaagct aggttgtggg aaactggaag ttagttttag aagttactat
    14281 tttttcttag tacctgttct aaccctgctt aaactagtaa aaagaactcc tacatccata
    14341 agtgacatag gagtttaggc ttatctttta ctgagttaca gttacttctc cagttcggta
    14401 atccagttga agtccctttt gaacattggg aactagaaca ttgaattcca attctccatc
    14461 cgaagacttg gcttcaatct gtgaagctga gggaactaaa tcctcgtttg aagcgtagta
    14521 aagggttaca cggtaacgga cacgcttgtt ttggtccaag gctttacgca ccttgctttc
    14581 atagtagttt tgaccagtcg aatactcggc ttgtgcctga tttgcccagg ctgtctgaac
    14641 agcaatgttt ttaggattgc ttgttgaggc atcaaaacca tccaaaccac cgattaaggc
    14701 atagcctaac aaatgacctc tatcgactgc atgggtataa gagcccttta gattcttgac
    14761 ctgatgccaa cctggaggag tccaagaagt tgaaccattc ccagtttctt tacgattctt
    14821 gtactgacga gtggccttag acaagagggc attagctacg gttggaacag tttccttgcc
    14881 cactgtcttt gttttattgt cagcgtaggg cttacttgaa accttggcat ctagatttgt
    14941 tttattacca ttgacgataa aagcacctga gccattccac tccagactcc cctttatttg
    15001 actcttgact gcgtctgtta agacactctc tgccaatgct tgactaggag cttcagacgc
    15061 ttgttttttc tgactaagct tggttttagg actattagct gtcgactgca tctgcttgat
    15121 ataatagctc cctgtagaca aaagcaataa cactagcagt ccgattagtg tctgtcttgt
    15181 ttttttgttc atatttctcc ttatctttag aaaaaggctg gttctccagc ctaatttcct
    15241 gtaaatttat gaatcaattc ctgccatttt gctggagaca ggatagccca tggatcttga
    15301 cccttgccca agattccata acctaccatt aaaccgattc ctagagccag agtacctaaa
    15361 atcagtacta tgatgactaa aagtaaacgc ttgactacgt agcttgattt cttattcatc
    15421 ttcatcactt gcctcaatcc gctgaatctt agcacctagc tgcgccaact tctcatggaa
    15481 accgtagtaa cctctatcca agtgaaccaa tttaccgacc acagtttctc cctgtgctac
    15541 caaacctgtc aaaatcaagg ccgcactggc acgaaggtca gttgaaagaa cttctgctcc
    15601 ctgcaaaggc tgtccaccaa caatacgagc tgtatcacgg ataatctcag aatgcaagcc
    15661 catgcggcgc atctcttcta ggtgttggaa acgattttca aaaactgtct ccaccatggt
    15721 tgattcgcct tttgcaactg tcatcaaggc tgtaaattga gcctgcatat ctgttggaaa
    15781 tcctgggtgg ggcaaggttt tcacatgaac agcttttaga ttttccagtt gagaacgaac
    15841 acgaattcct tcgtcttctt caattacttc aacacccatt tcaagtaact tggcaatcaa
    15901 gggacggttg tgctcccaga cagcgtctcg aatcaagaca tcaccaccag tcatggcagc
    15961 agctaccata aaggttcctg cttcgatacg gtcctggact acattgtgag tcgtaccatg
    16021 aagtttctca acaccagtaa tggttatagt ctctgtacca gcacctttga ccttggctcc
    16081 catttcatta aggagaatgg ctaagtcaac aatctcaggc tcacgcgcag cattctcaat
    16141 cactgtcacc ccatcagcca gagtcgctgc catcatcaag ttctgcgttg caccaacact
    16201 tggaaagtcc atatagatat gagcgccatg caagcgttct gccttggctt cgatgtaacc
    16261 agctgtctga ctaaccttaa cccccatagc ttccagacct ttcaaatgaa gatcaatagg
    16321 acggctacca atcgtacaac cacctggcat ggataccttg gcatgaccca cacgggcaag
    16381 gattggccct aatacaacga tggaggcgcg catcttgctg acatacttgt aaggggcttc
    16441 ctcagtgatg tcgccagtag catccacctt gacaagatga gcttcctcat caaagtcaac
    16501 cttggcattc aaaccaccaa ctacctgatt cataataaag acatccgaca aaatcggaac
    16561 attctgcaag acggtctttc cttcacttgc tagaatagtc gctgccaaca agggtaagac
    16621 tgcatttttt gctccctcga tcgtcacgct tcctaccaga cgattatcgc caccttgaac
    16681 cacaatttta tccatactcg tttcctttac tcttgatttt ataataatcc tctaagcgct
    16741 tcttgccaca aatgatacag gctaataaag aaagaactca ggatgtagcc tatgacaatg
    16801 ctaaaaaagg ctactaataa acgaactttt cttgtattct cagctgtcac ttttaaaacc
    16861 ttttcccatc taacaagatc ctttaaaagg taaaaactca cataaataaa gagcatatga
    16921 ctacttagag tgaataataa ttgaaccatt tgactattat accatcttct ctgtttgtgc
    16981 gctagtttgg aaacttcact caaaaattag ggattgtttt tcaagtaatc aatcgcatct
    17041 tgtagggttt taacgggcac gattttcata tccgtcttga ttgttttagc cgcttctagg
    17101 gctgtttggt agttgttttt cgcgtccgga tgcgcctttt gttcttcttc gctaacaggg
    17161 ttatcaggag caaagaaaat agcagcacct gccctagccg aagctacaac cttcttatca
    17221 atacctccaa tgtctcccac attaccatcg cggtcaatgg tacctgtacc ggcaacaata
    17281 cgaccattac gaagatctgg gtgagctatt tgagtataga tagctagact aaacatgaga
    17341 ccagcacttg gaccgccaat accagctgtt gaaaagctaa ttgggacatt gctgattacc
    17401 tctgtacggt caatcaagcc gattccaatt ccatttttgc cattttccaa ggtgataatc
    17461 tttccttctg cagacttggt ttgcccatcc tcttcatagg tgaccttgac ggagtcccct
    17521 aatttttgag aactgacgta atcaatcaag tctttggaac tatcaaaggt ctgatcattg
    17581 actgctgtga ctgtatcaga gatattgaga atccctttaa aggttgaatt atccgtcaca
    17641 ttcaaaacat aaactccaaa gtacttgagt tcgatttcct taccagctgt ttttagtcct
    17701 tgatacttgg ccatattttg cgatgtttgc atgtagaatt gattgattcg cataaattca
    17761 acatcggaag aaccacctgt agtctcctga gcactacgaa tatctgtaaa aggtgtcaac
    17821 caagcataaa tcatatgagc taaagtagca tgttgaacac caaccgtaac gaattgatag
    17881 gcaccagctt ccttatcttc tgtgtcattt actttaagga cttggcgaat atcttccgaa
    17941 ccacctggaa cctctatata gtagggcaaa ggcaccacaa atgccaagaa ggtcacaatc
    18001 aaggccgcaa tgacatacaa aggccatcta atcttttttt tcatttctta tttcctccaa
    18061 aatactctcg ggaacatggc aggcaatatc ctgaccaaac ttcaaaagct ctcgaacacc
    18121 tgatgaactg atatagagat gttcaggccg gctatgtaaa taaatagtct ctatatcaga
    18181 agacagctga tgattgtagt aatcaaaact agcttcatat tgcaaatccg ccgcattcct
    18241 caacccccgt actagaaaag tagcccccag tctttttgca acatcgacca ccaatttatc
    18301 atgagaagcc acgacttcaa cattttccaa atgtcccaaa gccttttcta gcccccgttt
    18361 acgattttca agagggagaa atccttgttt gtggggatta aaaaaaatac ccacataaag
    18421 cttatcaaaa agtctgctcg cccgttcaat gatatccaga tgcccatttg tcatcggatc
    18481 aaatgagcct gtgaataagc caatcttatc tgacatagac tgtcacctta ctaattccat
    18541 aaatcttttc cttccagata cccagacagg caatttcttc tggaagttca acggctttat
    18601 ccgtctcgca cacaaccata acatcttcag aaaaaagctc tctctcagcc attttttcaa
    18661 tatctgctac gatttgttcc ttggcatagg gagggtctaa gaaaacgagg tcaaattccc
    18721 cagatacctg ttccaatgcc ctttctgcat ccatctttag gagttgaaat tttccaactt
    18781 ccttggtcat ctggatattt tcagccacga tggtctgagc cttacggtct cgctccacca
    18841 aaacagcact ggacatgcca cgcgatactg cttcgataga taaaccacca ctacctgcat
    18901 aaaggtccaa gactcgtccc acttcaaagt agggaccaat catgttaaaa atggctcccc
    18961 taaccttatc cgaagtaggt cttgtcgtct tgccttctag tgtcttgagg ggacgtcccc
    19021 catagattcc tgatacgatt ttcatactgt ttattatacc aaattataga caaaaagaga
    19081 aagaaaaccg aaccttgcgg ttcgattctc tacaaaatat tttcgtaagt atcgcggact
    19141 tcttgaggcc aaacacttgt ttgcacttct ccgatgtgtc tcttgcgaag taggaacatg
    19201 gccatacgag attgtccaat tcctccaccg attgtcaatg ggaatagacc attcaacaaa
    19261 gacttgtgcc attccaattc caagcggtct tcatcacctg taatttccac ctgacgtcta
    19321 agagtttctt catctacacg aattcccata gaagacaact caaaggctcc acctaaagac
    19381 tcattccaga caagaatatc accatttaga cccttgtagc cattctcaga ctcgcttgtc
    19441 cagtcatcat agtctggtgc acgtccatcg tgcggtttac catctggcaa ctcgccaccg
    19501 ataccaatca aaaagacggc tccaaattct ttacaaatcg cattttcacg ttctttcggt
    19561 gtcaagtctg ggtagcgttc taccaattct tctgtatgga taaaggtgat ttgttttggc
    19621 aagatagact cgatgtcata gcgggcttca acagctagct cagtcaggcg aatagcctta
    19681 taaatcttct caactgtttc ttttagataa acgatgttac gcttaccatt tgggataacc
    19741 ttctcccagt cccactggtc aacataaaca gagtgggttg catccaagga atcctcatct
    19801 ggacgaaggg ctttcatgtg gacaaagaga ccctctcctt caccaaagcc aaaacgagcc
    19861 aaggtgtggc gtttccattt agcaagtgag tgcaccactt cataagtagc atcagggatt
    19921 tggagaacct tgaccgatac tggattttcc acaccagaca ggttgtcctg cattccgtca
    19981 ccgaccttac tcaagatagg accttgaact tcgacaactt ctagcttatc tttcaaatac
    20041 tgggtaaaag tgtttttgac aaaggaaatt tcttcttgtt gatggataaa acttttcttc
    20101 atagacaact cctcaaaaat ttaataaacg tattatacac ctatttccaa gaaaagaaaa
    20161 gagaaaattt aaaaaaaatc agtgtcactc aaaacactga tttcatagac cttttagtca
    20221 ttgcgaccaa agatacgtag aatacttagg aaaagattga taaaatcaag atagatgctg
    20281 agagccattg acaccaccca gcctgtcgct acacgacctt gtgattgttc ataggcgagg
    20341 cgaatctttt ggttatccca agcaatcaac cctgaaaaaa ccaaaaccat ggctacgcta
    20401 atcatataat caaagaagcc actagccaag aaaatattga ctaccatagc aatcagtaaa
    20461 ccaatgagag ctgccatcat tgctcgacca atcccactca agtctttctt ggtaaagata
    20521 ccaactgccg ccatgacaaa gaagagaagg gcgctcgata caaaggcaga taaaactgta
    20581 cccggagtat agaaagccac cacaaaacta agggtaaagc cgtttaaaac ggagtaaagt
    20641 aaaaatactg gaagagcagc tggactattt cttgaagcca tgctactggc aacgaaaacc
    20701 aaagccaact ccgcaaaagt agcaatggtc aaccagagac gcccctgcat caaaaagtaa
    20761 accaactgag actgaaaaac cgtcaacata aggcctgata ccaaagcaga tagtccgatt
    20821 cccagaccaa caaaggcata aaccttagcg taaaattgat tgagacctgc acggtcatgt
    20881 ataatagtgt gattcatcca tttctccttt tctgtgaaca tctttccttg attataacaa
    20941 aaaaagttaa aattagctta aatagaagat taaaataccg gctgcaaccg caacattcaa
    21001 actctcggcc tgccccttca tgctaatatg gaccaactgg tctgcacttt cagccataag
    21061 gggactaatt ccttgaccct catttcccat aactagtaca aaattttcta tagaaggcag
    21121 ttctctgtaa tcaacagaat ctttagatag ggttgttgct agcactggga tagctacctt
    21181 tttagtttcg tcaagaagcg cttgactagt catccggtaa atgggcagat ggaaatgact
    21241 accttgcatg gaacgtaggg ttttgagact gtagatgtct gccgacttat ctgaaacaat
    21301 cactcctgta aaacctgctg catccgcagt tcgaatgata gttcctacat taccaggatc
    21361 ttgcacatct tccaaaaaca agaacttgcc ctgactcaaa tcagcttgtc ctacttcttc
    21421 tttttgaacc acggcaacaa ttccctgtgg agtctgagaa tccgccaaat ctagcaaaat
    21481 atcctctgaa atccagacag tttgcggaaa agcagctaac tgatttcgat aattttctag
    21541 ggcaaagatt ttctcaatcg tcactccagc ttggacagct tcttcaaaca agtgccagcc
    21601 ttcaatcaaa taggcagact tgcggtattt tttttggtgt aatttcttgg cattttttac
    21661 cacagaattg gcttttgagg ttataatagt catagaaata ttataacaca atcaaagggg
    21721 tttggtatgc aaaaggttag aatgattgcc caaggtaggg tacagggagt cggctttcgt
    21781 tggggtgttt acagcttggc acttgaaatt ggtggcatca caggtcgagt atggaataac
    21841 gacgatggca cagtggaaat cttagcccaa gcagactcat ctgctatcat ggcaaaattt
    21901 atccaagaaa tccgaaaagg accgacacct ttttcaaaag taagctactt agatgtcaaa
    21961 ctaagcaact ttcctcccta ctatgacttt aaaatcgcaa attaggtctc cagaactatt
    22021 gtatattttg taaaaaaaca gtagaataga aaggtataat ttttaaagaa ggaataaaaa
    22081 cagtgaaatc tattaaacgt tttgcactct cggctatggg agtggctatg ttgcttgtct
    22141 tgactggctg tgtcaatgtc gataaaacca caggtcagcc aacaggattt atttggaata
    22201 cgatcggagc gcctatggct gaagctatca agtacttcgc tactgataaa ggtctaggct
    22261 ttggtgtcgc tatcatcatc gtaaccatta tcgtgcgctt gattatcttg ccacttggta
    22321 tctaccaatc atggaaggca acgcttcact ctgaaaagat gaacgctctc aagcatgtcc
    22381 ttgagccaca ccaaacacgt ctcaaagaag caactactca agaagaaaaa ctcgaagccc
    22441 aacaagccct ctttgccgct caaaaagagc acggcatcag catgtttggc ggtgtaggat
    22501 gtttccctat cctccttcaa atgcctttct tctctgctat ctactttgct gcccaacata
    22561 ctgaaggggt tgctcaagca agctacctag gcattcctct aggttctcca agtatgattt
    22621 tggttgcctg tgctggtgtc ctttactatc ttcaatcgct cctttcactt cacggagtag
    22681 aagatgaaat gcaaagagaa caaatcaaga aaatgattta catgagccca ctcatgatcg
    22741 tcgtcttctc cctcttctca ccagctagtg tcacacttta ctgggttgtc ggtggtttca
    22801 tgatgattct ccaacagttt atcgtcaact atatcgttcg tccaaaactt cgcaaaaaag
    22861 tccgtgaaga actagccaag aacccaccaa aagcaagtgc tttctctaaa ccaagtggac
    22921 gaaaagacgt tacccctgaa caaccaactg ctatcacaag caagaaaaaa cacaaaaatc
    22981 gcaacgctgg aaaacaacgt tcgagataag aagaaaaagg cttagctagt ttgctaagtc
    23041 ttttttgcaa gtcatactct tcgaaaatca aattcaaacc acgtcagctt cgccttgccg
    23101 tactcaagta cagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatca
    23161 aaagcccgat gtttccatca ggcttctttt ctatccatgt acacgtttca tataatcttg
    23221 ataactttcg gtatccatga gttttttagc gttcttgacg cgatctgctg ttggtggttt
    23281 gactccttcg agggaatatg gaattccaag ttcacgccac ttgaactcac ccatggtgtg
    23341 ataaggtaga atttcaaact tatcaacatt tttgagggtc ttgacgaact taccaagttc
    23401 aatcaagtca tcatctctgt ctgtcaatcc tggaactagc acgtggcgaa tccagacagg
    23461 ttttccaata tctgatagat actgggcaca agccaagata tttttattag tttggctagt
    23521 gacaatcttg tgctgttctt cgttgatttc cttgatatcc aaaagaacca agtcagtgac
    23581 agccatgagt ttgtcaaact tctcaaggta acgtggttta ttacggaaag gaagagcaca
    23641 ggtatccaag gtacagtgga ttccttgttc cttagccttg gtgaagagag caatcaggaa
    23701 atcaatctgc aagagagctt ctcctccact gactgtaatc ccacccttat ttccccagaa
    23761 accacggtag cgcaaggcct ctgtcaagac atcatctacc gtccgttcac gtgacttatt
    23821 ggactccata gcccaagtgt ctgggttgtg gcaatactgg caacgcatgt gacagccctg
    23881 caaaaagaca ataaagcgaa taccaggccc atctactgac ccaaagcttt ctgtcgaatg
    23941 caccattcct gtcacttgtc cataatcaat tgtttcttca gacatatcgt taccttcctt
    24001 gaaaacgttt tatagtttta ttatatcacg acttgaagga aaaacaagat ttttgcctat
    24061 tttttagaaa gcaatctgtt tttcaatttc attttcaatc tattatcaga ttattcttca
    24121 aaatcgaagc catataaggt tgcaaaatag tcctcagggc gctctgcacg gcggatttga
    24181 cgggctttac cttcttcggt atagaggatt tccgcagaac gtaatttggc attatactgg
    24241 tagcccattg aaaatccgtg ggcacctgta tcatgaatta ccagcaaatc accgatttct
    24301 gtatgaggca gttcgcgatt gactgcaaat ttatcattgt tttcacagag tgaaccaacc
    24361 acatctaccg cttcagctgg tccatctgga tgggtcacgt tgctaatatg atggtaagct
    24421 ccgtacatag ctggacgcat gaggttgact gctgaggcat ccacacctag ataggtacgg
    24481 taggtttcct tcttatgagt cactcttgtg actagagcac cgtgaggtgc cagcataaaa
    24541 cgacccaatt cggtgaaaat cttgacctga ccaagacctg ctggcgtaag gacctcttca
    24601 tacaccttac gaactccctc accaatcaag gcgatatcgt tcggctcctg gtctggacga
    24661 taattaacac caataccgcc agaaagattg ataaagtcta gccaaatgcc caacttttcc
    24721 ttgatttcaa cagccagttc aaagagctga cgagccaact ctggataata gagatgggtc
    24781 acggtattgg acgctaggaa ggagtgaagc ccaaaagtct tggctccttt ttccttcaag
    24841 atagcaaagg cttcaaagag ctggtccttg gtcatgccaa acttagcctc cccaggatta
    24901 tccataatgt ctgtccccag ttcaaaaacg cctccaggat tataacgaca agagatgatt
    24961 tctggaatgc ctgctactct ctccagatgt tcaatatctt caaaggcatc caagttaatg
    25021 gtcgcaccca attcacgtgc ataggcgtat tccttgtctg gcgtgttgtt ggaagagaac
    25081 ataatctcag aacccagaaa gtccagttta tggctcatca aaagctctac ataactagag
    25141 cagtccacac cacaaccttc ttcttggaga attttcaaaa tagctggagt tggagtagcc
    25201 ttaactgcaa aatattcctt aaagcccttg ttccacgaaa aagcttggtt gacggctctt
    25261 gccttctcac gaatcccctt ctcatcatac aagtgaaagg gagtcgggaa ctcggcaaca
    25321 atcgcttcta actcttctct attgataaat ggtgttttca taagcttcct tctattctgt
    25381 ttgcaaagaa aggctgggac aatcgttcca acctttagtt ctatctctta tttatcttcg
    25441 tcaaagatat tgccaacctc aacatcgatg gcttggttct tgacatcaat attggttacc
    25501 ttcaagagtg acttgtagtc aaactgcttt tcacgttctt cttgggcatt gtccgcaaag
    25561 accgctacac ctgccagttc tgagtcgaac tcgcgcaaga gactaatcat accattgacc
    25621 gttccgccac ctttcaagaa gtcatccaca atcaagacac ggctgcctgc cttaagacta
    25681 cgttttgaaa ggaacatttt ctcgatacgg tcaccacttg aacctgaaac atagttgacg
    25741 ctaacagttg aaccttcggt aattttcagg tcacggcgca caatgacaaa agagacattg
    25801 aggacattgg caactgcatt tgcaagtggc acacccttag ttgctacggt cataaccgcg
    25861 tcaatttttt ggtccataaa gcttttggca ataatacgac caatattttt caagatggct
    25921 ggtgtgctaa gcaaatcaga cagatagata taaccacctg gcaagatacg gtcactttct
    25981 gacaacttgg tacgcaagtc ttcaaccatt tccttagcat cctgactcga aatagacggt
    26041 gtgaagatga cacctccgcc agccccagtc actgtctgga tatgaccgat ttcaatttcc
    26101 tcaaaggcgc gtttgataat gacgatatct tctgagatgg atgatttagc agactcatac
    26161 ttttcagcaa aagtattgag actagttagt ttataaggat tattaatcaa atagttggaa
    26221 atgacaacca tccgatcact tcttcttaat ttcatttcta tttcctattc catttttatt
    26281 caaaaaatca aaaagcaaac tagaaagctg gtcgctggtg gttcaaaaca ctgttttgag
    26341 attgtcaata gaactgacaa accctgtaat atacctgcat atatacatac gacaaggcga
    26401 tactacccta gtttgaagag attttcgaag agtattcatt tttgtctttt acttattata
    26461 ccatattcac ataaaaaaac gaacattctt atcctaaaaa atgctcattt ttcttaaatt
    26521 attaatctaa atctggttta tagaaggaac gattatccat agcgaagatt ttattggtca
    26581 tctctccttt atccaccaaa gcaagagctg ttgacatcat catcatgctt gcatccagat
    26641 tgtcaatcat atggataatc tctgcttcca taatgcgtgg acggactggg cttccatact
    26701 caagcaagcc gtggtgactg aggatgacat gacgaagcaa aacgacttct tccttggtat
    26761 catcgatgcc gagttccata actgtcttgg taatttcgct atcaatgaga gcgatatgtc
    26821 caagaagatt acctcgcact gtgtactctg tctggtctgg ccccgtcaac tcgataacct
    26881 tagctaagtc atgcagcata atccccgcat agagtaagct cttattgagc tgaggataaa
    26941 cttcgctaat agcgtctgcc aaacgtacca tggtcgccgt atgataagcc aagcccgttt
    27001 caaaggcatg gtggttggtc ttggcagctg gataggagta gaattcctta tcatacttgg
    27061 tgtagagatt tcggacaatc cgttgccaga caggattttc aattttgaaa atcatttgcg
    27121 acatgtagtc acgaatttcc ttgacatcaa ctggtgactt gaccttgaaa tcggctgggt
    27181 cattgggttc accagcttga ggcaggcgga gagtaatttg attgacttga ggggtattgt
    27241 tataaacttc tcggcgtcct ttcatgtgga caaccttacc tgcggtaaag gcctcaatgt
    27301 tatgaggttg ggcatcccag agcttcccat caatctcgcc actatcatct tggaaggtaa
    27361 aggctaggta gtttttccca gctcgagttt gcctcaggtc agctgatttg attaggtaaa
    27421 agccttcaaa tagctcatct tttttcatgt gactaatctt catattcttc ctcattttct
    27481 tgaaaatgga gtagatcaag cgcaggctcg ccttctgaca actcaatgtg acggagcgtc
    27541 cgctcgatag ctatggtacg acggtttaat aattcatcaa tattgccaga ggcatgttgg
    27601 agatgttttt gtgccttgac cagaatacca ccaaacttgc caaactcggt cttgacactg
    27661 gcaagagtct tgctgatatg gtcggcactc ttttggatat taagggtctt gaaaccaact
    27721 gatagggagt taagaagggc tgatagggta cttggtcctg caacaataat ctgttcttcc
    27781 cgtctcaaat catcaaagaa gaccggattg cgcacaatct ctgaataaag accttctgtc
    27841 ggaacaaaca agattccaaa gttggttgtc cgaggcggtg ctatgtactt gctcttgata
    27901 tccttggcaa agcgcttgac gcttgctagg agtgacttac gacagcgttc aatctcatcc
    27961 ttgtcacctg tctcataggc ttcttccaag cggtaataat ctgccagtgg aaacttagag
    28021 tcaattggca gatagacgta ttcttggtcg ccttgtccgg gtaacttgat ggcatactcc
    28081 actcgttcac tagagttttc aaccgttgcg tattctcgtt cgtactgggc aggtgtcatg
    28141 atgtcttcaa taatttgccc cagttgcaat tctcccagaa tccctcgcgt cttggttcca
    28201 gagagaacct tgttaagagc tccgacatca cgggcaactg tctgcatttc tccaaggcca
    28261 cgattgacag actccagttg tttagaaact gtctcaaagg aagcctgtaa gcgtgtctgc
    28321 aaggtctttt ctagtttttc ctcgaccgtc tggcgcattt gttccaaacg ttgctcattt
    28381 gattcctgca aggcttggag acgttggtct gtcttgtctc tagtttggag gagattatct
    28441 gtcatttctt gacggacttg ggtcagacct tggtgcaatt caatccgcac ttcttgcaaa
    28501 cggtcgctga caaccacttc caaatctttt tggtctaact ggctggcttg tctggcttgg
    28561 tcaaagcggt aatccaactg gtctgacaaa tgatctgcct gatcctccaa gctcttactt
    28621 aagtgtttct cctgcctatc ctgcctttgc caaatcagaa aaagaccagc taggttggca
    28681 attaataata gaataagtaa actttccatc ctacctcctg tccttgctat gcagtacgac
    28741 cacataacca tctgggcaag ttaccgacac ttccctatct atatattcgt tagaagcgta
    28801 cactttttta aagaaaaaat tctcctctgt caactcatac ttggctccaa gaatagtcag
    28861 ctggctatcc cgaactggca taaaggctag atagtcgtag tctgaacgag gttctagctg
    28921 actggtccct tctgaacaat aggcaatcaa attttgccca tcctcaattt ctatttgacg
    28981 catatagggt gccaacttag gattgctagg tagaaagaca ttggccaaca tatggtcaat
    29041 acggccaccc aaggcaccga aaatagtgac ctcagcctga ggattttgtt caaagatggt
    29101 taagagagcc aattccagat ctgtatcatc cttttctggt cgtgcttgga caaaatactg
    29161 ggcacgtttt tgaatcacct gtcgctcttc ttccgtcaca gaatcaaaat ctccgactgc
    29221 tagagcaaga ggtaagtctt cttccaagac ccagagcgag cctcgatcca ccccaacaaa
    29281 agcatcaaaa tctgtccgat aatgaccgcg gtttccgcct gcaaaaactg caaccctagt
    29341 ccagttgttt tctgagagtt tgtactcgct cattgacttc tcccttaaag acataggaac
    29401 ctgctacaaa aacagtcgca ccagcttctt tggcttgggc aatagtttgg tcatcaatcc
    29461 caccatccac ttcgatttca aagttcaaac ctttttcctc acgaagagca accaactcac
    29521 gaaccttatc catggtttct ggcagaaagg cttgcccacc aaaacctgga ttaaccgtca
    29581 tgactaaaac ttggtcaact agatgaagga cgtgcttgat ggcttcaact ggtgtgccag
    29641 gattgataac gactgaaggc ttaactccga gtgaacgaat tttttggagg gcgccatgaa
    29701 tatgaggtgt tgcttctaca tggatactga tgatgtctgc acctgcacgc gcaaaatctt
    29761 ccagatgatg ctcagggttt gacaccatca agtggcaatc gaaaaccatc ttactatgag
    29821 gacgaaggct ctcgaccaca cctgcaccaa aactgatttg cggtacaaaa tgactgtcca
    29881 tgatatcgat atgggcatat tctgccccag ttgcttctag acgtttgatt tcacgttcaa
    29941 agttggcata atctgctgcc agaattgacg gagcaatctt gtattgagac ataggtttct
    30001 ccttattttg gaattttttt gctgactttt ttataggttt ctctacgatt ttcaatttcg
    30061 ctgaggaatt gcaggtagtt gtcaaaacgg aaggtggcaa tggtcccctc ttctacagct
    30121 ggcttgacgg cacaagacgg ctcatgggta tgggtacaag tacgaaactt acaatctcgg
    30181 ctgacagtag caatctctgg gaaagcctga ttgaggtctt cagcccttga tacttcatag
    30241 tccagagatg aaaagcctgg tgtgtctgcg attttacccc cgttgagatt gtaaaaacta
    30301 acagctcgag tggtatggcg accgcgacct agactgtctg aaatttctcc cgtttcaaga
    30361 ttgaggtctg gtacgagttt attgagaaga gttgacttcc caacacctgt ctgccccata
    30421 aagaccgtaa ccttgcctgt taacaaagac aggagttcct ctttactggt cacaaagtca
    30481 tagccgatgt caccataggt ctgccggtaa aaatccagtt ctcccctatc ttccaacaaa
    30541 tccattttgg aaatatagac aatgggatgg atgcccttgt gctccaaaag aaccaagaaa
    30601 cgatccagca aattgctgtt aaaatcaggt tccttgacgg acatgattac tacagcttga
    30661 tcgatattga caataggcgg acgaaccaga ctgtttttcc gttcgtgaat tttgaggata
    30721 tagccttctg aattttcctc ggcagagaaa tctacccagt ccccaacata aggggtatgg
    30781 ccttttttac ggaaattccc acgcgcgcgt gtttgataaa cctggccatc actctccaca
    30841 tagtagaaac ctgccaaggc tttaatgatt tgtccctgca tcttagagtc cttgcccttt
    30901 aagtgcgtct gctagaccgg caaattctgc caagctgaga gcttccccac gcacacttgg
    30961 tgacaagcct gcctggtcca aagccttggt cagcttgtcc ttgacctctt cagtcttacc
    31021 aaagtaacct gtcaagttat tccacaaggt cttgcggcga tgggtaaaac tagccttgga
    31081 aaccttaaag aaaaagttct catcttctac tgctacggct ggctctggac gacgcaccat
    31141 tttcaagatg gctgaatcca catttggcgc tggcacaaag accgtacgag gcacgataaa
    31201 ggcaaccttg gctgtcatgt aatactgcac ggcgatagac aagctaccgt aagccttggt
    31261 gttaggctgg gctgaaatgc ggtccgctac ttctttctgc atcatgacca caaactcaca
    31321 aaaaggaatg ccactctcaa tcaagtgcat gagaataggc gtcgtgatgt agtaaggcaa
    31381 attagccact accttgattg gcaggtcagg atttttaaaa ttctggatat gttgcgccaa
    31441 atcaaccttg agaatatctt cgttaactac ggtcacatta tcaaaatcac gcagggtatc
    31501 tgccaaaatt ggcaccaaac ggtggtcaat ctcaaaagcc atgacttggg ctgcacgctc
    31561 agccaaaaat tctgtcaagg caccaatacc tggcccgatt tcgatgacat tgacctgatc
    31621 atcaatttca gccgtatcca caattttttg aaggatattg gtatccgtca aaaaattttg
    31681 cccaaaggac tttttaaagg taaaaccgtg acgctccagc actgccttgg tcacgctata
    31741 atctgcaatt ctcatctatt ctcttttcta ttatctgtta ctactattgt aacacattcc
    31801 ccttgtattt tgggaatgaa aatcatacca aaaaactatc tttcctcatg aagaaagata
    31861 gtcttggaaa tctttaaaag tggccttagc agttctctcg aaacatgcgt tcaaaaaaat
    31921 cagcttcttg ataacttcga ttttcaggta gggtcaaatc cacatcctcc tccacatcat
    31981 ccactgtcag ttcaaccgtc tgaggtaaat ttttcaaacg ataatgcttt tgcctttgcc
    32041 cttcaatact agcaaaatca agcaaatagc catgcactcg gatttgaacc aaaaggagat
    32101 gattggtcat attttccatg atgaggatat cttctaaacc acctctggca ctaggataat
    32161 cagataccct gcgaatacgg attttttcac acatgagcaa gtttcctcat atcaatgatt
    32221 tcatcagcct tattccagac taggggatta tgggtcgcaa tgatgataat tcgattttca
    32281 tctttcaaat ccaccaagag attcataacc tcctctgaat tttcaggatc aagagctgct
    32341 gttggttcat ctgccaaaat caagggtgga tttttcaaaa tagtcttagc aagggcaact
    32401 cgttgggcct ctcccccaga taaagtatag attttttgtt ctaaatccaa ataccctaga
    32461 ttaacttttt ctaaagcccc cacttgcctt tccaaacgtt ctacttttga gattttctga
    32521 ccaacaaaac ccaaatccaa attttctttg attgattggt tttctaagag gccaaaattt
    32581 tgaaagagat agcccatctg atctcgaaaa tactcacgag tgggaatggt ttttaaatct
    32641 ttcccctgat agagaaccct tcctccatct atcttttcta gctttcccaa gatattcagc
    32701 agcgtcgtct ttccgcttcc actctttccg attaaggcat aaaccttgcc cttctccagc
    32761 ttgagattca aaccagagaa aatcgcgcgg tcattaaatt tcttttccaa tccttgaata
    32821 tcaatcatct tattttcctt tcaatactgc catgctacca gcttcttctt ttttgtcctg
    32881 ccttactaaa atcaagaggg cgttaagcgt aaacaaagct acaactagag cgctaatcaa
    32941 accatctctt gtcaaaatac tagataggac taggccaagc aagagaactc ctccttgcgc
    33001 cagtaaatac ttgccatgaa gctcatagat tgtcatacca gcaagacgtt taatcataag
    33061 ctcccgtctg aactgctcaa aatagagaag attcatggaa tcaaataaca agatagccgt
    33121 agacagggtc aaaatcgtcc caataaggag agagtaaaac tccacccgtt tgtcatttag
    33181 taccttggca aaaaatagct ggctttttac caagtaagaa accttgtgat acagaccttg
    33241 ctcttttaga gctgtgatgc tgtctccata tcgatctagt ttcaaggcac tattagccgt
    33301 agttccccac aacatccctg caacaggtct tgttccagtc gcttgcggcg ttagaactac
    33361 aataatcgga tcttttaggt actggcgtgt tgtcttgtac ccatcattat agaggaaacg
    33421 ttcctgtcct gtttctgtaa aagctagcct tacctgtggg aagtagtgtt tctgactcgt
    33481 cacttctaca ctttcagatg aaaagttttg caggtaatct gtaaacaatc cttggtagta
    33541 ggcagactgc tcccgcaagc tctcaggcaa gatcagccca aactctccct cagacaagtt
    33601 ttgcatcttg tcaataaact ctgaagaaac ggtaatcttt tcttctatca gatagcgcgg
    33661 cgagacatag ataacattcc ctgacggtgt gtagtcactg agacgattgc catctaggtc
    33721 cacttctgct ccatctgaga aattgtcaac attgctcata atataaaaag agtctgtatt
    33781 ggctaaccgt tcttcagtaa atgtctgcca ctcacgatta tccttacgcg ttccttcttc
    33841 atcggcaaat gcactagacc aaccaaagga tagacggtaa cggtctgagg actggctcca
    33901 tttattgcta gctctctcca tttcctgcat ttcacggtag tggggtagga gagctgtcgc
    33961 actcgatccg accaccaata cagctaagag ttgccccacc atcatcaatg tcatcatccg
    34021 tttgagaggg agtttccctt tcaatagatc caccagacta ttttcctgta aaccaagtag
    34081 atagacgaca cttagtaagg tagaaatccc tgccagggtc aatccataaa gtagaagagc
    34141 aatgatgacc agttgcaccg ttgccataaa caaggcacct tgataccaga gaatccccaa
    34201 tcccaaaaga ctggatacca gcactgagca gataagctgt ctcacatctt ctaacactgg
    34261 tctgagagca actccaaaca agctctcacc agctattaag cgaatccctg cctgacgaag
    34321 ggatttgatc cgataaatca gggtcagact cataaaggtc agcagaaaaa tagctaaact
    34381 cagtagcacc ataggggtgg ccgtcaatag taagactatc gaaaatggat cttctccatt
    34441 cgaaacaaag ccttgataac caagctcttt caaggtggtc tgtaagctca ctccatccaa
    34501 acttcctgac acaatcaagt aagaccctaa taaatcactc gtttctgcac tctccttgga
    34561 ggaaatggtc agaccttctg gaagctttcc ctcaccataa gtggcatagg taaagcgtgt
    34621 ttctccagct tcatttggct caacaatccg acgggcgatg agactattat gctctaccgc
    34681 caagtgactc aaagcctctg tcacctcttc gcggctagct actggttgat ttatcatcat
    34741 tcctgtcaca tcaacagaag gatagttacc aaataaaatc tcttctcgat ggtcaacgga
    34801 agttatcacc atataaagcg ataccaatac cattgaaatc aaaataaata aacgtttcat
    34861 catttcctcc aaaaagttac ttggtcactt agtgagaagg tgaccaagta aagctaaaca
    34921 atagggctat accattctaa acattatagt a
//
LOCUS       DAVLYV010000013        32100 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLYV010000013 DAVLYV010000000
VERSION     DAVLYV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.013"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..6394
                     /locus_tag="VYO70_000656"
     CDS             <1..6394
                     /locus_tag="VYO70_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1712928.1"
                     /translation="VILAAYAPNEVVLADTSSSEDALNISDKEKVVLDKETENKEKHE
                     DIHNAIETSKDTEEKKTTVIEEKEVVSKNPVIDTKTSNEEARIKEDSNQSQGDNAHSS
                     ANKGTENPKKEDRLVYIAEFKDKESGEKAIKGLSNLKNTKVLYTYDRIFNGSAIETTP
                     DNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGKNFDGRGMV
                     ISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAFNYYNGGKIT
                     VEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYKMYSDAGSG
                     FAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGIPMVVATGN
                     YATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDKVNIGGESF
                     KYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIAVMDRIYTK
                     DLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVFSISGDDGV
                     KLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEIDFKFAPDT
                     DKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTYGYMSGTSM
                     ATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQNTARPMMDATSWK
                     EKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKEIKGDKKYF
                     TIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPDGKQIVPEIHPEKVKG
                     ANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEALNSNGKKI
                     NFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGKPKIPGTLNKGIGG
                     EHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNEGINAPSSSGSKIANIYPLDSNG
                     NPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVISREHFIRGILNSKS
                     NDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQFEPIAEGQY
                     FYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYHKVKDQYKN
                     ETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQGRNRKLDKD
                     GNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANQADEKGMISYYLVDPDQDS
                     SKYQKLGEIAESKFKNLGNGKEGSLKKDTTGVEHHHQENEESIKEKSSFTIDRNISTI
                     RDFENKDLKKLIKKKYKEEDDFVTGGKRTVELDYKYDDKGNITAYEDESALEYETEKF
                     DEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKFIEIKATKYDSHSKTMT
                     FDLYANINDIVDGLAFAGDMRFFVKDDDRIKAETKIRMPEKNKETKAEYPYVSSYGNV
                     IELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVTTYNPGKTD
                     MLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLMSALDGFNII
                     RYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAIKEDGSMLF
                     IDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNESVVDNYLI
                     YGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQTGYSDLNA
                     KAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDGEIQEQHIY
                     VNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSELKPHRVTVT
                     IQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVINLSKDTFI
                     KPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHSQKSDSTKD
                     VTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGLKKKNQD"
     gene            7021..7497
                     /locus_tag="VYO70_000657"
     CDS             7021..7497
                     /locus_tag="VYO70_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1712929.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEKLYAYIAEATA"
     gene            7535..7840
                     /locus_tag="VYO70_000658"
     CDS             7535..7840
                     /locus_tag="VYO70_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1712930.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            7888..9363
                     /locus_tag="VYO70_000659"
     CDS             7888..9363
                     /locus_tag="VYO70_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1712931.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLLIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            9386..9463
                     /locus_tag="VYO70_000660"
                     /pseudo
     CDS             9386..9463
                     /locus_tag="VYO70_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            9713..16414
                     /locus_tag="VYO70_000661"
     CDS             9713..16414
                     /locus_tag="VYO70_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1712932.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDTKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKTLRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGQTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVTAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKNLTVEWEEVPAEN
                     LIKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(16662..17518)
                     /locus_tag="VYO70_000662"
                     /pseudo
     CDS             complement(16662..17518)
                     /locus_tag="VYO70_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(17729..18088)
                     /locus_tag="VYO70_000663"
     CDS             complement(17729..18088)
                     /locus_tag="VYO70_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1712933.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRTGFL
                     LTLIMYIEMIVELFLMK"
     gene            18180..19136
                     /locus_tag="VYO70_000664"
                     /pseudo
     CDS             18180..19136
                     /locus_tag="VYO70_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            19120..19677
                     /locus_tag="VYO70_000665"
     CDS             19120..19677
                     /locus_tag="VYO70_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1712934.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALKKTQERLHQAGLTNAQLILQGHETLDQFVTKAKAGIFNLGYLPSADKSI
                     ITRPQTTIEALGKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            19670..19924
                     /locus_tag="VYO70_000666"
     CDS             19670..19924
                     /locus_tag="VYO70_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712935.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKDQKEIFESCCKKD"
     gene            19936..21990
                     /locus_tag="VYO70_000667"
     CDS             19936..21990
                     /locus_tag="VYO70_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1712936.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLSLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            22169..23029
                     /locus_tag="VYO70_000668"
     CDS             22169..23029
                     /locus_tag="VYO70_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1712937.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSINFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQFLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            23240..23950
                     /gene="ccdA2"
                     /locus_tag="VYO70_000669"
     CDS             23240..23950
                     /gene="ccdA2"
                     /locus_tag="VYO70_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1712938.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            23970..24536
                     /locus_tag="VYO70_000670"
     CDS             23970..24536
                     /locus_tag="VYO70_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1712939.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            24547..25659
                     /gene="msrB"
                     /locus_tag="VYO70_000671"
     CDS             24547..25659
                     /gene="msrB"
                     /locus_tag="VYO70_000671"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1712940.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            25882..26619
                     /locus_tag="VYO70_000672"
     CDS             25882..26619
                     /locus_tag="VYO70_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1712941.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            26616..28307
                     /locus_tag="VYO70_000673"
     CDS             26616..28307
                     /locus_tag="VYO70_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1712942.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDARGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VTFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYHMKIDSRPPKGTKVEIYINRIETS"
     gene            28423..28986
                     /locus_tag="VYO70_000674"
     CDS             28423..28986
                     /locus_tag="VYO70_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1712943.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            29008..>32100
                     /locus_tag="VYO70_000675"
     CDS             29008..>32100
                     /locus_tag="VYO70_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1712944.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPKTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQLSDKSAEES
                     KVEQVGEPVEPSEDEQAPTAPVEPEKQPEAPEEEKAVEETPKPEDKIKGIGTKEPVDK
                     SELNNQIDKASSVSPTDYSTASYNDLGPVLETAKGVYASEPVKQPEVNSETNKLKTAI
                     DALNVDKSELQEQLRVAEQKQQADYSAKTWREFKIAELQAKEINNPTTPLPKQSEIDA
                     ATKALQDALQALAVDKTVLQNAINTANSKREEEYTAQTWKALKDALTAVNPVNEDETA
                     TQSKVDEATRNLEEAINNLVLLTEKPVLTFIETDKKALEREVVAKYSLENQNKTKIKS
                     ITATLKKGETVVSTVELIGDDVTNETITSAFKNLEYYKEYTLSTTMVYDRGDGDVTEI
                     LDNQPIQLDLKKVELKNIKRTDLIKYENGKETNESLITTVPDDKRNYYLKITSKNQKT
                     TLLAVKNIEETTVNGTPVYKVTAIADNLVSRTADNKFEEEYVHYIEKPKVHEDNVYYN
                     FKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYITKEFTGKLLSSEGKQFAITELEH
                     PLFNVITNATINNVNFENVEIERSGQDNIASLANTMKGSSVITNVKITGTLSGRNNVA
                     GFVNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAGTNYRGIVRKAYVDATITGNKTR
                     ASLLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVSNPVEVGAIASKTWPVGTVSNSV
                     SYAKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGYSSGNRSFRKSKTFTKLTKEQAD
                     A"
BASE COUNT        10542 a         5631 c         6849 g         9078 t
ORIGIN      
        1 agttatttta gcagcctatg cccctaatga ggtagtctta gcagacacat ctagctctga
       61 agatgcttta aacatttctg ataaagaaaa agtagtatta gataaggaaa cagaaaataa
      121 agagaaacat gaagatattc ataatgctat agaaacttca aaggatactg aagaaaagaa
      181 aacaacagtt attgaggaaa aagaagttgt tagtaaaaat cctgtgatag acactaaaac
      241 tagcaatgaa gaagcaagaa tcaaagaaga ttccaatcaa tcccaaggag ataatgcaca
      301 ctcgtctgca aataaaggca cagaaaatcc gaaaaaagaa gatagacttg tctatattgc
      361 tgaatttaaa gataaagaat ctggagaaaa agcaatcaag ggactatcaa atcttaagaa
      421 tacaaaagtt ttatatactt atgatagaat ttttaacggt agtgccatag aaacaactcc
      481 agataacttg gacaaaatta aacaaataga aggtatttca tcggttgaaa gggcacaaaa
      541 agtccaaccc atgatgaatc atgccagaaa ggaaattgga gttgaggaag ctattgatta
      601 cctaaagtct atcaatgctc cgtttgggaa aaattttgat ggtagaggta tggtcatttc
      661 aaatatcgat actggaacag attatagaca taaggctatg agaatcgatg atgatgccaa
      721 agcctcaatg agatttaaaa aagaagactt aaaaggtact gataaaaatt attggttgag
      781 tgataaaatc cctcatgcgt tcaattatta taatggtggc aaaatcactg tagaaaaata
      841 tgatgatgga agggattatt ttgacccaca tgggatgcat attgcaggga ttcttgctgg
      901 aaatgatact gaacaagata tcaaaaactt taacggcata gatggaattg cacctaatgc
      961 acaaattttc tcttacaaaa tgtattctga cgcaggatct gggtttgcgg gtgatgaaac
     1021 aatgtttcat gctattgaag attctatcaa acacaacgtt gatgttgttt cggtatcatc
     1081 tggttttaca ggaacaggtc ttgtaggtga gaaatattgg caagctattc gggcattaag
     1141 aaaagcaggc attccaatgg ttgtcgctac gggtaactat gcgacttctg cttcaagttc
     1201 ttcatgggat ttagtagcaa ataatcatct gaaaatgacc gacactggaa atgtaacacg
     1261 aactgcagca catgaagatg cgatagcggt cgcttctgct aaaaatcaaa cagttgagtt
     1321 tgataaagtt aacataggtg gagaaagttt taaatacaga aatatagggg cctttttcga
     1381 taagaataaa atcacaacaa atgaagatgg aacaaaagct cctagtaaat taaaatttgt
     1441 atatataggc aaggggcaag accaagattt gataggtttg gatcttaggg gcaaaattgc
     1501 agtaatggat agaatttata caaaggattt aaaaaatgct tttaaaaaag ctatggataa
     1561 gggtgcacgc gccattatgg ttgtaaatac tgtaaattac tacaatagag ataattggac
     1621 agagcttcca gctatgggat atgaagcgga tgaaggtact aaaagtcaag tgttttcaat
     1681 ttcaggagat gatggtgtaa agctatggaa catgattaat cctaataaaa aaactgaagt
     1741 caaaagaaat aataaagaag attttaaaga taaattggag caatactatc caattgatat
     1801 ggaaagtttt aattccaaca aaccgaatgt aggtgacgaa aaagagattg actttaagtt
     1861 tgcacctgac acagacaaag aactctataa agaagatatc atcgttccag caggatctac
     1921 atcttggggg ccaagaatag atttactttt aaaacccgat gtttcagcac ctggtaaaaa
     1981 tattaaatcc acgcttaatg ttattaatgg caaatcaact tatggctata tgtcaggaac
     2041 tagtatggcg actccaatcg tggcagcttc tactgttttg attagaccga aattaaagga
     2101 aatgcttgaa agacctgtat tgaaaaatct taagggagat gacaaaatag atcttacaag
     2161 tcttacaaaa attgccctac aaaatactgc gcgacctatg atggatgcaa cttcttggaa
     2221 agaaaaaagt caatactttg catcacctag acaacaggga gcaggcctaa ttaatgtggc
     2281 caatgctttg agaaatgaag ttgtagcaac tttcaaaaac actgattcta aaggtttggt
     2341 aaactcatat ggttccattt ctcttaaaga aataaaaggt gataaaaaat actttacaat
     2401 caagcttcac aatacatcaa acagaccttt gacttttaaa gtttcagcat cagcgataac
     2461 tacagattct ttaactgaca gattaaaact tgatgaaaca tataaagatg aaaaatctcc
     2521 agatggtaag caaattgttc cagaaattca cccagaaaaa gtcaaaggag caaatatcac
     2581 atttgagcat gatactttca ctataggcgc aaattctagc tttgatttga atgcggttat
     2641 aaatgttgga gaggccaaaa acaaaaataa atttgtagaa tcatttattc attttgagtc
     2701 agtggaagaa atggaagctc taaactccaa cgggaagaaa ataaacttcc aaccttcttt
     2761 gtcgatgcct ctaatgggat ttgctgggaa ttggaaccac gaaccaatcc ttgataaatg
     2821 ggcttgggaa gaagggtcaa gatcaaaaac actgggaggt tatgatgatg atggtaaacc
     2881 gaaaattcca ggaaccttaa ataagggaat tggtggagaa catggtatag ataaatttaa
     2941 tccagcagga gttatacaaa atagaaaaga taaaaataca acatccctgg atcaaaatcc
     3001 agaattattt gctttcaata acgaagggat caacgctcca tcatcaagtg gttctaagat
     3061 tgctaacatt tatcctttag attcaaatgg aaatcctcaa gatgctcaac ttgaaagagg
     3121 attaacacct tctccacttg tattaagaag tgcagaagaa ggattgattt caatagtaaa
     3181 tacaaataaa gagggagaaa atcaaagaga cttaaaagtc atttcgagag aacactttat
     3241 tagaggaatt ttaaattcta aaagcaatga tgcaaaggga atcaaatcat ctaaactaaa
     3301 agtttggggt gacttgaagt gggatggact catctataat cctagaggta gagaagaaaa
     3361 tgcaccagaa agtaaggata atcaagatcc tgctactaag ataagaggtc aatttgaacc
     3421 gattgcagaa ggtcaatatt tctataaatt taaatataga ttaactaaag attacccatg
     3481 gcaggtttcc tatattcctg taaaaattga taacaccgcc cctaagattg tttcggttga
     3541 tttttcaaat cctgaaaaaa ttaagttgat tacaaaggat acttatcata aggtaaaaga
     3601 tcagtataag aatgaaacgc tatttgcgag agatcaaaaa gaacatcctg aaaaatttga
     3661 cgagattgcg aacgaagttt ggtatgctgg cgccgctctt gttaatgaag atggagaggt
     3721 tgaaaaaaat cttgaagtaa cttacgcagg tgagggtcaa ggaagaaata gaaaacttga
     3781 caaagacgga aataccattt atgaaattaa aggtgcggga gatttaaggg gaaaaatcat
     3841 tgaagtcatt gcattagatg gttctagcaa tttcacaaag attcatagaa ttaaatttgc
     3901 taatcaggct gatgaaaagg ggatgatttc ctattatcta gtagatcctg atcaagattc
     3961 atctaaatat caaaagcttg gcgagattgc agaatctaaa tttaaaaatt taggaaatgg
     4021 aaaagagggt agtctaaaaa aagatacaac tggggtagaa catcatcatc aagaaaatga
     4081 agagtctatt aaagaaaaat ctagttttac tattgataga aatatttcaa caattagaga
     4141 ctttgaaaat aaagacctaa agaaactcat taaaaagaaa tataaggaag aagacgactt
     4201 tgtaactggt ggaaaaagaa cagtagaact tgattataaa tatgacgata aaggaaatat
     4261 aactgcctat gaagatgaaa gtgctctaga atatgaaact gaaaaatttg acgaaataaa
     4321 atcaaaaatt tatggtgttc taagcccatc taaagatgga cactttgaaa ttcttggaaa
     4381 gataagcaat gtttctaaaa atgccaaggt atattatgga aataactata aatttataga
     4441 aatcaaagcg actaagtatg actcacattc aaaaacgatg acatttgatt tatacgctaa
     4501 tattaatgat attgtggatg gattagcttt tgctggagat atgcggtttt ttgttaaaga
     4561 tgatgatcga ataaaagctg aaactaaaat tagaatgcct gaaaaaaata aggaaactaa
     4621 agcagaatat ccctatgtat caagttatgg gaatgtaatc gaattaggag aaggagatct
     4681 ttcaaagaac aaaccagaca atttaactaa aatggaatct ggtaaaatct attctgattc
     4741 agaaaaacaa caatatctgt taaaggataa catcattcta agaaaaggct atgcactaaa
     4801 agtgactacc tataatcctg gaaaaacgga tatgttagaa gggaatggag tctatagcaa
     4861 ggaagatata gcaaaaatac aaaaggccaa tcctaatcta agagtccttt cagaaacaac
     4921 aatttatgct gatagtagaa atgttgaaga tggaagaagt acccaagctg tattaatgtc
     4981 ggctttggac ggctttaaca ttataaggta tcaagtgttt acatttaaaa tgaacgataa
     5041 aggggaagct atcgataaag acggaaatct tgtgacagat tcttctaaac ttgtattatt
     5101 tggtaaggat gataaagaat acactggaga ggataagtcc aatgtagaag ctataaaaga
     5161 agatggctcc atgttattta ttgataccaa accagtaaac ctttcaatgg ataagaacta
     5221 ctttaatcca tctaaatcta ataaaattta tgtacgaaat ccagaatttt atttaagagg
     5281 taagatttct gataagggtg gttttaactg ggaattgaga gttaatgaat cggttgtaga
     5341 taattattta atctacggag atttacacat tgataacact agagatttta atattaagtt
     5401 gaatgttaaa gacggtgaca tcatggactg gggaatgaaa gactataaag caaacggatt
     5461 tccagataag gtaacagata tggatggaaa tgtttatctt caaactggct atagcgattt
     5521 gaatgctaaa gcagttggag tccactatca gtttttatat gataatgtta aacccgaagt
     5581 aaacattgat cctaagggaa atactagtat cgaatatgct gatggaaaat ctgtagtctt
     5641 taacatcaat gataaaagaa ataatggatt cgatggtgag attcaagaac aacatattta
     5701 tgtaaatgga aaagaatata catcatttga tgatattaaa caaataacag acaagacact
     5761 aaacattaag attgttgtaa aagattttgc aagaaataca accgtaaaag aattcatttt
     5821 aaataaagat acgggagagg taagtgaatt aaaacctcat agggtaactg tgaccattca
     5881 aaatggaaaa gaaatgagtt caacgatagt gtcggaagaa gattttattt tacctgttta
     5941 taagggtgaa ttagaaaaag gataccaatt tgatggttgg gaaatttctg gtttcgaagg
     6001 taaaaaagac gctggctatg ttattaatct atcaaaagat acctttataa aacctgtatt
     6061 caagaaaata gaggagaaaa aggaggaaga aaataaacct acttttgatg tatcgaaaaa
     6121 gaaagataac ccacaagtaa accatagtca attaaatgaa agtcacagaa aagaggattt
     6181 acaaagagaa gatcattcac aaaaatctga ttcaactaag gatgttacag ctacagttct
     6241 tgataaaaac aatatcagta gtaaatcaac tactaacaat cctaataagt tgccaaaaac
     6301 tggaacagca agcggagccc agacactatt agctgccgga ataatgttta tagtaggaat
     6361 ttttcttgga ttgaagaaaa aaaatcaaga ttaagataaa agctatagaa aaaaatggtt
     6421 tatgtactga gattagatag tgaggtgatg acatagtttt gtgaaaacag ccatttataa
     6481 ctcaattatt tagtttactt cactattgat actatttgga gttattaatg gacttagttt
     6541 agataactaa tgaattgatt gaaagggtta gtattgacaa tattggtcat attgattaga
     6601 aaatagagtc tatcaaaatt taaaggctaa tagaggtgat gagacaattt caaggaatta
     6661 agtagcgttt ggttgcttaa ttccttttta tttggaagga agtttttcgt aaccgcccaa
     6721 taacgaagtc tattgggcgg ttacgatatg atcactgact tcgtcagttc tatctacaac
     6781 ctaaaaacag tgttttgaac aacctgcggc tagcttccta gtttgctctt tgattttcat
     6841 tgaatattag aacagcaaaa atgcttggag tatttgtttg tgtgtttgtt tttatataac
     6901 aaactataaa caaaacaaaa atataaaaaa agagacaaaa aagaacaaaa agtaattgac
     6961 aacggtttca tatagtgtta tacttatggc ataaagttaa tggaaagaag ggaaaacctt
     7021 atggtattag attatttctt tgacaaaaac cttgtgtttt gcttagaagc ggataatcaa
     7081 gaacaactct ttgatcaggt tgcaacttta ttggaagaac gagaaattgt cactccaact
     7141 tatcgtgaag ccttgatcac gcgtgaaaag tcatttccaa ctggtttaga tatggaattt
     7201 ctaggaaagg acttgccaaa tgtagcgatt cctcatacag atattgttca taatctagct
     7261 gagaaagtgg tggttgttcg attagaaaaa ccagtaactt ttcacaatat gatagctcct
     7321 gataaggaag tagaagtatc cttgctcttc tttatcatca ataactcaag ttcaagtcaa
     7381 acaaatattc tggctcagtt gatggacttt ttcacaggaa atggacatct tgaagaccta
     7441 tcaaaaattt ccgaaccaga aaaactttat gcttacattg ctgaagcaac cgcttaatct
     7501 tgtctattaa aaaataaaat cggaggaaat ctaaatgatt aaaattcttg ctgcctgcgg
     7561 tgcaggtgtt aactcaagtc accaaattaa aagtgctcta gaagaagaac tttcaaaccg
     7621 tggttatgat gttcactgtg atgcagtcat ggtgaaagat gtaaacgaag accttatgaa
     7681 aggttacgat atctttacac caatcgctgc aacagacctt ggttttgaac caggtatccc
     7741 agttatcgaa gctggaccaa tcttattccg tatcccagca atgagcgctc cagtatttga
     7801 caatattgaa gcagctatta aagaacacgg attaagctaa ttctaatttg ttaacattca
     7861 tataacataa aaaagggagg aactgttatg gatgcaatct ttgacctaat cggaaaggtt
     7921 ttcaatccca tcttagaaat gggtggacct gtcatcatgt taatcatttt gacagtattg
     7981 gctttacttt ttggagtgaa attctccaaa gcgcttgaag gtggtatcaa acttgccatc
     8041 gctcttacag gtatcggtgc tatcatcggt atgctaaatg gtgctttctc agcatcactt
     8101 gcaaaattcg ttgaaaacac tggtatacaa ttgagtatta ccgacgttgg ttgggcacca
     8161 cttgcaacaa tcacttgggg ttctgcttgg acactttact tcttgctcat catgttgatt
     8221 gtcaacgtag tgatgcttgc tatgaagaaa accgatacac ttgatgtcga tatctttgat
     8281 atctggcact tgtctatcac aggtcttttg attaaatggt atgctgataa caatggtgtg
     8341 agtcaagggg tttcactctt gattgctaca gcagctatcg ttcttgtcgg tgtgttgaaa
     8401 attatcaact ctgacttgat gaaacctaca tttgatgacc ttcttaacgc cccaagttca
     8461 tcaccaatga catcaactca catgaactac atgatgaacc cagttatcat ggttttggat
     8521 aagatttttg aaaaattctt cccaggcctt gataaatatg actttgatgc tgctaaattg
     8581 aacaagaaaa tcggtttctg gggatctaaa ttcttcatcg gtttcatcct tggtatcgtt
     8641 atcggtatta tgggaactcc acatccaatt gcaggtgttg cagatgcaga taaatggcgt
     8701 cttgttatca aaggatggtt gtctcttggt ttgactgccg gtgtatcttt ggaactcttc
     8761 tcacttatcg gttcatggtt catcgcagcc gtagaaccac tatcacaagg tattacaaac
     8821 gttgctacta aacgtcttca aggacgtaaa ttcaatatcg gtcttgactg gccattcatc
     8881 gctggtcgtg ctgaaatctg ggcttgtgcc aacgtacttg caccaatcat gttgattgaa
     8941 gcagtgcttc tttcaaaagt tggaaatggt atcttgccac ttgcaggtat catcgctatg
     9001 ggtgttactc cagctctctt ggttgtaact cgtggtaaat tgctccgtat gattatcttc
     9061 ggaacactct tgttgccact cttccttctt tcaggtacac ttattgcacc atttgcaaca
     9121 gaacttgcta aaggtgtagg tgccttccca gaaggtgtga gccaaactca attgattact
     9181 cactctactc ttgaaggacc aatcgaaaaa cttcttggtt ggacaattgg taacactaca
     9241 actggtgata tcaaagcaat ccttggtgca gtagccttcc ttgtattcta tatcggtatc
     9301 tttgcttggt acagaaaaca aatgatcaaa cgtaacgaag agtacgcagc aaaagcaaaa
     9361 taatgcgctc ctataaaata taaactcaat gaaaatcaaa gagcaaacta ggaagctagc
     9421 cgcaggttgc tcaaaacaca gttttgaggt tgtagataag actgacgaag tcagctcaaa
     9481 acatggtttt gaggttgtag atgaaactga cgaagtcagc tcaaaacatg gttttgaggt
     9541 tgtagatgaa actgacgaag tcagtaatca tatctacggc aaggcgacac tgacgaagtt
     9601 tgaattggat tttcgcagag tgtaaattgt tgaaactagg ttgtcatacc atgtatacca
     9661 cttgggcttt ggtagtagct aactgcacta aatataatat aaggagggtt ttatggggaa
     9721 aggccattgg aatcggaaaa gagtttatag cattcgtaag tttgctgtgg gagcttgctc
     9781 agtaatgatt gggacttgtg cagttctatt aggaggaaat atagctggag aatctgtagt
     9841 ttatgcggat gaaacactta ttactcatac tgctgagaaa cctaaagagg aaaaaatgat
     9901 agtagaagaa aaggctgata aagctttgga aactaaaaat gtagttgaaa ggacagaaca
     9961 aagtgaacct agttcaactg aggctattgc atctgagaag aaagaagatg aagccgtaac
    10021 tccaaaagag gaaaaagtgt ctgctaaacc ggaagaaaaa gctccaagga tagaatcaca
    10081 agcttcaagt caagaaaaac cgctcaagga agatactaaa gctgtaacaa atgaagaagt
    10141 gaatcaaatg attgaagaca ggaaagtgga ttttaatcaa aattggtact ttaaactcaa
    10201 tgcaaattct aaggaagcca ttaaacctga tgcagacgta tctacgtgga aaaaattaga
    10261 tttaccgtat gactggagta tctttaacga tttcgatcat gaatctcctg cacaaaatga
    10321 aggtggacag ctcaacggtg gggaagcttg gtatcgcaag actttcaaac tagatgaaaa
    10381 agacctcaag aaaaatgttc gccttacttt tgatggcgtc tacatggatt ctcaagttta
    10441 tgtcaatggt cagttagtgg ggcattatcc aaatggttat aaccagttct catacgatat
    10501 caccaaatac cttcacaaag atggtcgtga gaatgtgatt gctgtccatg cagtcaacaa
    10561 acagccaagt agccgttggt attcaggaag tggtatctat cgtgatgtga ctttacaagt
    10621 gacagataag gtgcatgttg agaaaaatgg gacaactatt ttaacaccaa aacttgaaga
    10681 acaacaacat ggcaaggttg aaactcatgt gaccagcaaa atcgtcaata cggacgacaa
    10741 agaccatgaa cttgtagccg aatatcaaat cgttgaacga ggtggtcatg ctgtaacagg
    10801 cttagttcgt acagcgagtc gtaccttaaa agcacatgaa tcaacaagcc tagatgcgat
    10861 tttagaagtt gaaagaccaa aactctggac cgttttaaat gacaaacctg ccttgtacga
    10921 attgattacg cgtgtttacc gtgacggtca attggttgat gctaagaagg atttgtttgg
    10981 ttaccgttac tatcactgga ctccaaatga aggtttctct ttgaatggtg aacgtattaa
    11041 attccatgga gtatccttgc accacgacca tggggcgctt ggagcagaag aaaactataa
    11101 agcagaatat cgccgtctca aacaaatgaa ggagatggga gttaactcca tccgtacaac
    11161 ccacaaccct gctagtgagc aaaccttgca aatcgcagca gaactaggtt tactcgttca
    11221 ggaagaggct tttgatactt ggtatggtgg caagaaacct tatgactatg gacgtttttt
    11281 tgaaaaagat gccactcacc cagaagctcg aaaaggtgaa aaatggtctg attttgacct
    11341 acgtaccatg gtcgaaagag gcaaaaacaa ccctgctatc ttcatgtggt caattggtaa
    11401 tgaaataggt gaagctaatg gtgatgccca ctctttagca actgttaaac gtttggtcaa
    11461 ggttatcaag gatgttgata agactcgcta tgttaccatg ggagcagata aattccgttt
    11521 cggtaatggt agcggagggc atgagaaaat tgctgatgaa ctcgatgctg ttggatttaa
    11581 ctattctgaa gataattaca aaacccttag agctaagcat cccaaatggt tgatttatgg
    11641 atcagaaaca tcttcagcta cccgtacacg tggaagttac tatcgccctg aacgtgaatt
    11701 gaaacatagc aatggacctg agcgtaatta tgaacagtca gattatggaa atgatcgtgt
    11761 tggttggggg aaaacagcaa ccgcttcatg gacttttgac cgtgacaacg ctggctatgc
    11821 tggacagttt atctggacag gtacggacta tattggtgaa cctacaccat ggcacaacca
    11881 aaatcaaact cctgttaaga gctcttactt tggtatcgta gatacagccg gcattccaaa
    11941 acatgacttc tatctctacc aaagtcaatg ggtttctgtt aagaagaaac cgatggtaca
    12001 ccttcttcct cactggaact gggaaaacaa agaattagca tccaaagtag ctgactcaga
    12061 aggtaagatt ccagttcgtg cttattcgaa tgcttctagt gtagaattgt tcttgaatgg
    12121 aaaatctctt ggtcttaaga ctttcaataa aaaacaaacc agcgatgggc agacttacca
    12181 agaaggtgca aatgctaatg aactttatct tgaatggaaa gttgcctatc aaccgggtac
    12241 cttggaagca attgctcgtg atgaatctgg caaggaaatt gctcgagata agattacgac
    12301 tgctggtaag ccagcggcag ttcgtcttat taaggaagac catgcgattg cagcagatgg
    12361 aaaagacttg acttacatct actatgaaat tgttgacagc caggggaatg tggttccaac
    12421 tgctaataat ctggttcgct tccaattgca tggccaaggt caactggtcg gtgtagataa
    12481 cggagaacaa gccagccgtg aacgctataa ggcgcaagca gatggttctt ggattcgtaa
    12541 agcatttaat ggtaaaggtg ttgccattgt caaatcaact gaacaagcag ggaaattcac
    12601 cctgactgcc cactctgatc tcttgaaatc gaaccaagtc actgtcttta ctggtaaaaa
    12661 agaaggacaa gagaagactg ttttggggac agaagtagca agagttcgta cattaattgg
    12721 taaagaacca aaaatgccga aaacagtagg ttttgtatac agcgatggta gtcgtgaaaa
    12781 acttccagta acttggtctc aagtcgatgt tagtcaagca ggagttgtga ctgttaaagg
    12841 tacagcaaat ggacgtgagg tagaggctca tgtcgaggtt ctagcaattg cgaaagaatt
    12901 accaacagtt aaacgtattg ctccaaatac tgacttgaat tctgtagaca aatctgtttc
    12961 ttatgttttg actgatggaa gtgtacaaga gtacgaagta gatagctggg agattacgga
    13021 agtagataaa gccaaacttt cagtagctgg atcacgtatt caaatgactg gtcagttagc
    13081 tggagaaact attcatgcaa cccttgtggt agaagaagga aatgctgcag cacctgtagt
    13141 gccaactgtt actgttggag gtgaggcagt aacaggtctt actagtcgac aaccaatgca
    13201 atatcgtact ctatcttatg gtgcccaact gccagaagtc acagcaagtg ctgaaaatgc
    13261 tgatgtgaca gttcttcaag caagcgcagc aaacggcatg cgtgcgagca tctttattca
    13321 gcctaaagat ggtggtcctc ttcaaaccta tgcaattcaa ttccttgaag aagcgccaaa
    13381 aattgctcac ttgagtttgc aagtggaaaa agctgacagt ctcaaagaag accaaactgt
    13441 caaattgtcg gttcgagctc actatcaaga tggaacgcaa gctgtattac cagctgataa
    13501 agtaaccttc tctacaagtg gtgaagggga agtcgcaatt cgtaaaggaa tgcttgagtt
    13561 gcataagcca ggagcagtca ctctcaaagc ggaatatgag ggagctaaag gccaagttga
    13621 actcactatc caagccaata ctgagaagaa gattgcgcaa tctatccgtc ctgtaaatgt
    13681 agtcacagat ttgcatcaaa aacctactct tccaacaaca gtaacggttg agtatgacaa
    13741 aggtttccct aaagctcaca aagtcacttg gcaagctatt ccgaaagaaa aactagactc
    13801 ctatcaaaca tttgaagtac taggtaaagt tgaaggaatt gaccttgaag cgcgtgcaaa
    13861 agtctctgta gaaggtatcg tttcagttga agaagtcagt gtgacaactc caatcgcaga
    13921 agcaccacaa ttaccagaaa gtgttcggac atatgattca aatggtcacg tttcatcagc
    13981 taaggttgca tgggatgcga ttcgtccaga gcaatacgct aaggaaggtg tctttacagt
    14041 taatggtcgc ttagaaggta ctcaattaac aactaaactt catgttcgcg tatctgctca
    14101 aactgagcaa ggtgcaaaca tttctgacca atggaccggt tcagaattgc cacttgcctt
    14161 tgcttcagac tcaaatccaa gcgacccagt ttcaaatgtt aatgacaagc tcatttccta
    14221 caataaccaa ccagccaatc gttggacaaa ctggaatcgt actaatccag aagcttcagt
    14281 cggtgtccta ttcggagatt caggtatttt gagcaaacgt tcagttgata acttaagcgt
    14341 tggcttccat gaagaccatg gagttggtgc accgaagtct tatgtgattg agtattatgt
    14401 tggtaagact gtcccaacag ctcctaaaaa ccctagtttt gttggtaatg aggaccatgt
    14461 ctttaatgat tctgccaact ggaaaccagt tactaatcta aaagcccctg ctcaactcaa
    14521 ggctggagaa atgaaccact ttagctttga taaagttgaa acctatgctg ttcgtattcg
    14581 catggtgaga gcagacaaca aactaggaac gtctatcaca gaggtacaaa tctttgcgaa
    14641 acaagttgcg gcagccaagc aaggacaaac aagaatccaa gttgacggta aagacttagc
    14701 aaacttcaac cctgatttga cagactacta ccttgagtct gtagatggaa aagttacggc
    14761 agtcacagca agtgttagca acaatggcct cgctaccgtc gttccaagcg ttcgtgaagg
    14821 tgagccggtt cgtgtcatcg cgaaagctga aaatggcgac atcttaggag aataccgtct
    14881 gcacttcact aaggataaga acttactttc tcataaacca gttgctgcgg ttaaacaagc
    14941 tcgcttgcta caagtaggtc aagcacttga attgccgact aaggttccag tttacttcac
    15001 aggtaaagac ggctacgaaa caaaaaacct gacagttgaa tgggaagaag ttccagcgga
    15061 aaatctgata aaagcaggtc aatttaccgt tcgaggccat gtccttggta gtgatcttgt
    15121 tgctgaggtc actgtacgag tgacagacaa actaggcgaa gctctttcag ataaccctaa
    15181 ctatgatgaa aacagtaacc aggcctttgc ttcagcaacc aatgatattg acaaaaactc
    15241 tcatgaccgc gttgactatc tcaatgacgg agatcattca gaaaatcgtc gttggacaaa
    15301 ctggtctcca acaccatctt ctaatccaga agtatcagcg ggtgtgatct tccgtgaaaa
    15361 tggtaagatt gtagaacgga ctgttgctca agccaaactt cacttctttg cagatagtgg
    15421 tacggatgca ccaactaaac tcgttttaga acgctatgtc ggtccagagt ttgaagtgcc
    15481 aacctactat tcaaactacc aagcctacga cgcagaccat ccattcaaca atccagaaaa
    15541 ttgggaagct gtgccttatc gtgcggataa agacatcgca gctggtgatg aaatcaacgt
    15601 aacctttaaa gctgtcaaag ccaaagctat gagatggcgt atggagcgta aagcagataa
    15661 gagcggtgtt gcgatgattg agatgacctt cctggcaccg agtgaattgc ctcaagaaag
    15721 cacgcaatcg aagattcttg tagatggaaa agaacttgct gatttcgctg aaaatcgtca
    15781 agactatcaa attacctata aaggtcaacg gccaaaagtc tcagttgaag aaaacaatca
    15841 agtagcttca actgtggtag atagtggaga agatagcctt ccagtacttg ttcgcctcgt
    15901 ttcagaaagt ggaaaacaag tcaaggaata ccgtatccag ttgactaagg aaaaaccagt
    15961 ttctgagaag acagttgctg ctgtacaaga agatcttcca aaactcgaat ttgttgaaaa
    16021 agatttggcc tacaagacag ttgagaaaaa agattcaaca ctgtatctag gtgaaactcg
    16081 tgtagaacaa gaaggaaaaa ctggtaaaga acgtatcttt acagcgatta atcctgatgg
    16141 aagtaaggaa gaaaaactcc gtgaagtggt agaagctccg acagaccgca tcgtcttggt
    16201 tggaaccaaa ccagtagctc aagaagctaa aaaaccacaa gtgtcagaaa aagcagatac
    16261 aaaaccaatt gattcaagtg aagctagtca aactaataaa gcccagttac caaatacagg
    16321 tagtgcggca agccaagcag cagtagcagc aggtttagct cttctaggtt tgagtgcagg
    16381 attagtagtt actaaaggta aaaaagaaga ctagaaattt tgataccttc tttatcatta
    16441 aagaccaaag aaaaactctt gtcctaaata gaagataaag agtctggaac aaaatagttc
    16501 tcaaccacaa aaaagctaga tatttccaat tgcggaactc tagcttttta attttgagtc
    16561 gttctcttat tgtatagtag attgaaacta gagtagtaca cttctacttc taaaacattg
    16621 ttagaaatcg atttgactgt ccttatcgat ttgtcctgtt cttatttcat ttcactatgt
    16681 tttaggaggt tattggccat aagtaccaat cccatgtcaa ttctcacttg acgcttgcct
    16741 ctcagattac atctcttgta acccaaacaa gcctttatct gcccaaagac aggttctaca
    16801 tcaatcttgc gttgagcgaa aatctgtcta tcttggggag ataaaagcgc ctgacattct
    16861 ttagctttca agttttgata gcgttcgttc atatacaatc ccttttgagg ggctgattca
    16921 ggttcatcag cgtagtaaac cttgatttcc tgttgaaagt ctgtctgtgt tttctggtac
    16981 ttgatatggt gaaaacgata gcaccagcca tcaggatgta tgtagctatc ctccttgtca
    17041 ttatagtgcc aattcgctaa gtttttagct gactgtttat accctctctt ttgttcctta
    17101 tcaaacatgg catatttaat cagaagggga gtgatttctt cttgaaaata gacttgtatc
    17161 tgttcttgaa gtttggcgga gaatttctct gtcgttttct tccacacaaa actatacttg
    17221 ttggcattgg attcaatctt agttccgtca ataaacaaac aatctaaggt cactaactct
    17281 tccatttttc aatttttcga ccagagaaaa tcccttgtga ataggcaaat agaagagtag
    17341 atacaagcat tttagggtga taagacgggc gaccaaaggc atgataggag gtgtagaagt
    17401 gacgttcctc caaggtattc accacttttt caatagtaaa gacgagatgg tcttgtggta
    17461 agaaggaact gatttctagt ggtaaagttg tttgatttgt gttatagtga atatgcatgg
    17521 gatagccttt ctaaatggtt tattaagcaa ttctatttta ccaagactat cgtaaccatg
    17581 caaaaaagca acggcaaatc cgttgccttt tttggatata agagactatt gtcccagact
    17641 cttttctttg tataaaattg tatagtgtta ttgtctatac tattttgtag agaaagcaaa
    17701 aatccctaga aaatgcttag ggattcgact atttcattaa gaacaattca acaatcatct
    17761 ctatatacat gataagggtt aataaaaatc ctgtacgcca gaagaatttg atgaatttag
    17821 gatagtaaaa gctgcgtttt tttaaaagga agaaaaagac gagaataatg gctaggaacg
    17881 agagggctag ccccagtcta gggaggaaat tatgggtaaa ggttttagct gtaatcaagt
    17941 aatactcaaa taccaagact ggaaaagcca aatccgcaaa gtttcgtcct agttttttta
    18001 atctaaaaag tttggttagg ataatgcaga cgactaaggt cagtatcaat aataaaatag
    18061 atgctaattt cattaaaatc atacccatat tgtatcataa aaaaaggcta atggaaacga
    18121 gaaacagagg aatttatagg aaagtcaggc ttttgagatt gtgggtgatt tttgttataa
    18181 tgaaagttat aaaatcttat gataccttga atgattatta tcgaaaactc tttggagaaa
    18241 agacttttaa agtccctatt gatgcgggat ttgactgtcc caatcgtgat ggaactgtgg
    18301 ctcatggagg ctgtactttt tgtacggttt ccggttctgg agatgccatt gtagccccag
    18361 atccgcctat ccgtgagcaa ttttataagg aaattgactt tattcatcgt aagtggccag
    18421 atgttcagaa gtatctggtt tattttcaaa attttaccaa cacccatgaa aaggtggaag
    18481 ttattcgaga gcgttatgaa caggctatca atgagccagg tgtggtagga atcaatattg
    18541 gaacgcgccc agactgttta ccagacgaaa ccatcgaata tttggctgag ttatcggagt
    18601 gcatgcatgt gacggttgaa ttgggcttgc agactactta tgaagcaacc tctgacctga
    18661 ttaaccgtgc gcattcttat gaattgtagg tggaaacggt caagcgtttg agaaagtatc
    18721 caaaaattga gattgtttcc catttgatca atggtttgcc tggtgaaacc catgagatga
    18781 tggttgaaaa tgtccgccgt tgtgtcacgg ataacgatat tcaagggatt aaactgcatt
    18841 tgctccatct tatgacaaat actcgtatgc aacgagatta ccatgaggga cgtttacaat
    18901 tgatgagtca ggacgaatat gtcagggtca tctgtgacca actggaaatt attcccaaac
    18961 atatcgtcat ccatcgaatc acaggagatg cacctaggga tatgctgatt gggcttatgt
    19021 ggagcctcaa taaatgggaa gctctcaaca gcattgagat ggagatgcga cgtcgtggaa
    19081 gtgttcaagg atgcaaggct gtaaaacagg agtttgaaaa tgaaaagacc acttgagatg
    19141 gcacatgatt ttttggctga ggtcgtgaca aaagaggatg tcgtagtgga tgcgactatg
    19201 ggaaatggtc atgacacgct ttttttagcc aagctagcca agcaagtcta tgcctttgat
    19261 attcagaagc aagccttgaa aaagacccaa gagcgtttgc atcaggctgg cttgacaaat
    19321 gcccagttaa tcttgcaagg ccatgagaca ctggaccagt ttgtgacaaa agctaaggca
    19381 gggattttta atctgggcta tttgccgtca gctgataagt ctatcatcac ccgaccgcag
    19441 acaacgattg aggcattagg aaagctatgt ggcttacttg tcaaaggtgg acgaattgct
    19501 attatgattt actatggtca tgaaggaggc gacctcgaga gagatgctgt cttggatttt
    19561 gtaatccagt tgaaccaaca agagtacaca gctgccattt accgaacttt aaaccaagtc
    19621 aacaacccgc cgtttttagt gatgattgaa aaattagaga gatacagaca tggataaaca
    19681 atacctacac gaaaagctgg atgccatgcg ccagaatttt gttgaatcaa cccaccacga
    19741 acgatcggtg ggcgtgctag accaagcaca tatgagcaaa aaaatgctta aaatcaagaa
    19801 aaaattagtt gctcttgaaa tggaacggtg ccagagaaaa attgagcaca aagactgttc
    19861 caagattgac caaaaaatca aagatcagaa ggagatattt gaatcctgtt gtaaaaaaga
    19921 ttaaggagga cgtgcgtgga attactgatt tacctcatcc tatttttact ggttttgatt
    19981 gtctcaagta caaccaataa gctcctgccc tttttgcctc tccctttggt gcagattctt
    20041 ttgggaattg tgattggtct ctttttaccc aatactgact ttcaccttaa tacggagttg
    20101 tttttggcac tggttatcgg acccttgctt ttccgagagg ctgaagaagc agatgttacg
    20161 gctattttaa aacactggcg aatcattgtt tatctcatat ttccagtgat ttttatctcg
    20221 accctgagtt tgggtggctt ggcccatctt ctttggttca gccttccctt ggcagcttgc
    20281 ttggctgttg gggcagccct tggtcctacg gacttggtgg cctttgcctc tctttcggag
    20341 cgttttagct ttcctaagcg cgtgtccaat attcttaagg gcgaaggact cttgaatgat
    20401 gcttctggtt tggtggcttt tcaggtagct ttgacagctt ggacaactgg agctttttct
    20461 ctggggcaag ctagcagttc gctcatcttt tcaatcctag gcggtttttt aattggattt
    20521 ttaacagcca tgaccaaccg cttcctccat accttcttgc taagtgtgcg cgcaacggat
    20581 attgccagtg aacttttatt agaattgagt ttgcctctag tgaccttctt tctggcagaa
    20641 gaagttcatg tttcaggtat tattgccgtc gtagttgctg gaattttaaa ggcaagtcgc
    20701 ttcaagaaaa tcacgctcct cgaagcccaa gtggatacgg tgaccgagac ggtctggcat
    20761 acagtgacct ttatgctcaa cggttctgtc tttgtgattt tagggatgga gctggaaatg
    20821 atagcagaac ctatcttgac caatccaatc tataatcctc tacttttatt gctatctctt
    20881 atcgccctta cctttgtcct ctttgtcatt cgttttatta tgatctatgg ctattatgcc
    20941 tatagaaccc gacgcctcaa gaaaaagcta aataagtata tgaaggacat gtttctcttg
    21001 accttttcag gtgttaaggg aacggtgtcg attgctacga ttctcttgat accaagtaat
    21061 ctagaacagg agtatcctct cttgcttttc cttgttgcag gtgtgacgct tgttagcttt
    21121 ttaacaggtc tcttggtctt gcctcatctt tctgatgaag aggaagaaag caaggattat
    21181 ctcatgcata tcgccatttt gaatgaagta acgctagagt tggaaaaaga gttggaagac
    21241 accagaaata aactttccct ttatgcggct attgacaatt atcatggacg tattgaaaat
    21301 cttattttaa gccaagaaaa ccaggatgat caagaagact gggctgcttt gaaactcttg
    21361 attcttagta ttgaaagtga tggtttggaa caggcctatg aagaggggaa cattagcaat
    21421 cgtgcttacc gagtttacca acgttatctg aaaaatatag aacaaggaat caatcgtaaa
    21481 cttgcctcaa gactgaccta ttattttctt gtttccttga ggattttacg ttttctcctt
    21541 catgaagttt ttactctcgg aaagaccttc cgtagctgga agaacaagga gcaaagccgt
    21601 ctccgtgctc ttgattatga ccaaattgca gagctctatc ttgccaatac agagatgatt
    21661 attgaaagtt tggaaaacct gaagggagtc tacagacgct ctttgattag ttttatgcag
    21721 gagtctcgtc ttcgagaaac agctattatc agcagtggtg cctttgtcga acgggttatc
    21781 aatcgtgtca aacccaacaa tatcgatgaa atgctaagag gctattatct ggagcgcaag
    21841 ttgattttcg aatacgaaga aaaacgattg attacaacta agtatgccaa gaaattacga
    21901 caaaatgtaa ataacttaga gaactattcc ttgaaggaag ctgccaatac cctgccgtat
    21961 gatatggtgg aattggtaag aagaaattag ttaatactct tcgaaaatct cttcaaacca
    22021 cgtcagcgtc gccttaccgt atgtatgtga ctgacttcgt cagtcttatc tacaacctca
    22081 aaacagtgtt ttgagcagct tgcggctagc tttctagttt gctctttgat tttcattgag
    22141 tataagatta taaatgaagg agtatgacat gaaaaagtgg tggaaagagc tgatagacaa
    22201 gcctttatta aaagcttttt tgcattatta tcaagcatca gatagtgagt tgaccagtgt
    22261 cgcagtagcc tactattggt tgatttcgat tttccccctg cttttggtgg tggtcaatat
    22321 cctcccctat tttcagattc ctgtgggaga atttctgggc tttatgaaag acgtcctgcc
    22381 cccaagcctc tatgaaggtg tggaaaaaat agccagagaa gtcttgacgc aaccttcaac
    22441 aggtttattg agtttttctg ttttatcggc gttatggagt ttttcaaaat ctataaattt
    22501 cttgcaaaaa gcttttaaca aggcttatgg cgtcgaaaag agtcgtggac ttatttccca
    22561 tcagatgcta agtctcctag tcagttttgg cttgcagttc ctctttgcgt ttgccttgtt
    22621 tttgatctta tttggtcaga tgattttggc tttacttgct cattattgga caaaagatgg
    22681 cattatctat caggctttac aagggttggc aggtcctctg atttacgccc tcctctttgc
    22741 tatcttggtc atgctttatt attttcttcc caatctatct aacaggaaaa ttagctatac
    22801 cctaccaggc agtgcctttg ttctcttagt gattttaggt ttactaactc tgttctctag
    22861 ttatctcaat tactatgtcc atcatttggt ggatgtccga attttaggtt ccgttcttct
    22921 tgttgttatg atgttttggt ttatcttgat tgctaaaatt gttatcctag gcgcggttat
    22981 caatgccagt atgcagagtt tgaaagatcc ggtctttaaa aaagactaac tatttatcca
    23041 ttacaaaagc gcatagtatc aaaactttat agaaccttga tactatgcgc ttttgtgctt
    23101 tgacagtttt ttgtatagtt tttgtccaag atttctaaat catctgatta aattcctatt
    23161 ttcccctatc attgtccctg ttttttcgaa aatggaagga gtataataaa tctatcaata
    23221 aaatgatagg aggaacttta tgggtcatat tttctttttt ctaagtgtct ttttggcagg
    23281 gattctatcc ttcttttctc cttgtatctt acctttgtta ccggtctata caggagtgtt
    23341 actagatgat aaggatggtg ctcaggcttc tagcggcaaa ttttcaatct cagttactag
    23401 tttattacga actctggcct ttatagcagg aatttccttt atatttattt tgttgggcta
    23461 tggagctggt tttttaggcg atttgcttta tgcttcttgg ttccaatatc ttactggggc
    23521 aattattatc cttcttggtt tgcaccaaat ggagattcta cactttaagg ggctttataa
    23581 ggaaaagagg ctacaactgc aaggacaggg gcaaaatggt aagggctata gtcaggcatt
    23641 tttattgggc ttgaccttta gttttgcttg gacgccttgc gtggggccgg ttctggggtc
    23701 tgttttggcc ttggcggctt caggtggttc aggagcttgg cagggagctg gtctcatgtt
    23761 ggtgtatacg ctgggcttgg cgctaccatt cttgcttcta gctctgacct ctagttatgt
    23821 tttgaaacat ttccgaaaac ttcatcccta tctcggaatc ctcaaaaaag tgggtggttt
    23881 tctcattatt gtgatgggac tcttggttct gtttggaaat gcttcaattt taagtcaatt
    23941 atttgaataa aatggaaagg aatatcaata tgaaaaaatg gcaaacatgt gttcttggag
    24001 caggttcgct cctttgtttg acggcttgtt caggcaagtc cgtgactagt gaacaccaaa
    24061 cgaaagatga aatgaagacg gagcagacag ctagtaaaac aagcgcagct aaagggaaag
    24121 aggtggctga ttttgaattg atgggagtag atggcaagac ctaccgttta tctgattaca
    24181 agggcaagaa agtctatctc aaattctggg cttcttggtg ttccatctgt ctggctagtc
    24241 ttccagatac ggatgaaatt gctaaagaag ctggtgatga ctatgtggtc ttgacagtag
    24301 tgtcaccagg acataaggga gagcaatctg aagcggactt taagaattgg tataagggat
    24361 tggattataa aaatctccca gtcctagttg acccatcagg caaacttttg gaaacttatg
    24421 gtgtccgttc ttacccaacc caagccttta tagacaaaga aggcaagctg gtcaaaacac
    24481 atccaggatt catggaaaaa gatgcaattt tgcaaacttt gaaggaatta gcctaggagg
    24541 tgtcttatga atgataagtt aaaaatcttc ttgttgctag gagtattttt tctagccata
    24601 accggtttct atgttctatt gatacgaaat gcagggcaga cagatgcctc gcaaattgaa
    24661 aaggcggcag ttagccaagg aggaaaagca gtgaaaaaaa cagaaattag taaagacgca
    24721 gacttgcacg aaatttatct agctggaggt tgtttctggg gagtggagga atatttctca
    24781 cgcgttcccg gggtgacgga tgccgtttca ggctatgcaa atggtagagg agaaacaacc
    24841 aagtacgaat tgattaacca aacaggtcat gcagaaaccg tccatgtcac ctatgatgcc
    24901 aagcaaattt ctctcaagga aatcctgctt cactatttcc gcattatcaa tccaaccagc
    24961 aaaaataaac aaggaaatga tgtggggacc cagtaccgta ctggtgttta ttacacagat
    25021 gacaaggatt tggaagtgat taaccaagtc tttgatgagg tggctaagaa atacgatcaa
    25081 cctctagcag ttgaaaagga aaacttgaag aattttgtgg tggctgagga ttaccatcaa
    25141 gactatctca agaaaaatcc aaatggctac tgccatatca atgttaatca ggcggcctat
    25201 cctgtcattg atgccagcaa atatccaaaa ccaagtgatg aggaattgaa aaagaccctg
    25261 tcacctgagg agtatgcagt tacccaggaa aatcaaacag aacgagcttt ctcaaaccgt
    25321 tactgggata aatttgaatc cggtatctat gtggatatag caactgggga acctctcttt
    25381 tcatcaaaag acaaatttga gtctggttgt ggctggccta gttttaccca acccatcagt
    25441 ccagatgttg tcacctataa ggaagataag tcctacaata tgacgcgtat ggaagtgcgg
    25501 agccgagtag gagattctca ccttgggcat gtctttacgg atggtccaca ggacaagggc
    25561 ggcttacgtt actgtatcaa tagcctctct atccgcttta ttcccaaaga ccaaatggaa
    25621 gaaaaaggct acgcttattt actagattat gttgattaat actcttcgaa aatctcttca
    25681 aaccacgtca gcgtcgcctt accgtatgta gggttactga ctccgtcagt tttatctaca
    25741 acctcaaagc ggtactttga gaaacctgtg gctatcttcc tagtttactt tttgattttt
    25801 attgagtata agaggtcttt cctaagcagt tagaggagaa ttttgctata ctgatactag
    25861 taagtggtaa aggagcagat catgacctac acaatcttaa tcgtagaaga tgaatatctg
    25921 gtaagacaag gtttgactaa actggtcaat gtagcagcct acgatatgga aatcatcggt
    25981 caggctgaaa atggaaggca ggcttgggaa ttgatccaaa agcaggtgcc agatatcatt
    26041 ttaaccgata tcaacatgcc tcatctaaat ggcatccagt tggccagtct ggtacgagaa
    26101 acctatcctc aggttcattt ggtcttttta acaggttacg atgattttga ttatgccttg
    26161 tctgctgtca aactaggtgt ggacgactac ctgctcaaac ccttttctcg tcaggatatt
    26221 gaggaaatgt tggggaaaat caaacaaaaa ctagacaagg aagagaaaga agagcagtta
    26281 caagatttat tgactaatag gtttgaagga aacatggccc agaaaatcca gtctcatctg
    26341 gctgatagcc aatttagttt aaagtcttta gccagtgact taggttttag tccgacctat
    26401 ctgagttcct tgattaagaa agagttgggc ttgccttttc aggattatct ggtgagagaa
    26461 cgtgttaaac aagccaagct cttgctttta actacagatc tgaagattta tgagatcgca
    26521 gagaaggttg gttttgaaga tatgaactat tttacccaac gttttaagca gattgcaggt
    26581 gtgacacctc gtcagtttaa gaagggagaa gaccgatgaa gcgttcttct cttttagtta
    26641 gaatggttat ttccatcttt ctggtctttc tcattctcct agctctggtt ggaactttct
    26701 actatcaatc aagttcttca gccattgagg ccaccattga gggcaacagc caaacgacca
    26761 tcagccagac tagccacttt attcagtctt atatcaaaaa actagaaacc acctcgactg
    26821 gtttgaccca gcagacggat gttctggcct atgctgagaa tcccagtcaa gacaaggtcg
    26881 agggaatccg agatttgttt ttgaccatct tgaagtcaga taaggacttg aaaactgttg
    26941 tgctggtgac caaatctggt caggtcattt ctacagatga cagtgtgcag atgaaaactt
    27001 cctctgatat gatggctgag gattggtacc aaaaggctat tcatcaggga gctatgcctg
    27061 ttttgactcc agctcgtaaa tcagatagtc agtgggtcat ttctgtcact caagaacttg
    27121 ttgatgcaag gggagccaat cttggtgtgc ttcgtttgga tatttcttat gaaactctgg
    27181 aagcctatct caatcaactc cagttggggc agcagggctt tgccttcatt atcaatgaaa
    27241 accatgaatt tgtctaccat cctcaacaca cagtttatag ttcgtctagc aaaatggagg
    27301 ctatgaaacc ctacatcgag acaggtcagg gttatactcc tggtcacaaa tcctacgtca
    27361 gtcaagagaa gattgcagga actgattgga cggtacttgg cgtgtcatca ttggaaaagt
    27421 tagaccaggt tcggagtcag ctcttgtgga ccttgcttgg ggccagtgtc acatctcttc
    27481 ttgtctgtct ctgcttagtg tggttcagtc ttaaacgctg gattgctcct ttgaaggatt
    27541 tgagagaaac catgttggaa attgcttctg gtgctcaaaa tcttcgtgcc aaggaagttg
    27601 gtgcctatga actgagagaa gtaactcgcc aatttaatgc catgttggat cagattgatc
    27661 agttgatggt agctattcgt agccaggaag aaacgacccg tcagtaccaa cttcaagccc
    27721 tttcgagcca gattaatcca catttcctct ataacacttt ggacaccatc atctggatgg
    27781 ctgaatttca tgatagtcag cgagtggtgc aggtgaccaa gtccttggca acctatttcc
    27841 gcttggcgct caatcaaggc aaggacttga tttgtctctc tgacgaaatc aatcatgtcc
    27901 gccagtatct ctttatccag aaacaacgct atggagataa gctggaatac gaaattaatg
    27961 aaaatgttac ctttgataat ttagtcttac ccaagctggt cctacaaccc cttgtagaaa
    28021 atgctcttta ccatggcatt aaggaaaagg aaggtcaggg ccatattaaa ctttctgtcc
    28081 agaaacagga ttcgggattg gtcatccgta ttgaggatga tggcgttggc ttccaagatg
    28141 ctggtgatag tagtcaaagt caactcaaac gtgggggagt tggtcttcaa aatgtcgatc
    28201 aacggctcaa acttcatttt ggagccaatt accatatgaa gattgattct agacccccaa
    28261 aagggacgaa agttgaaata tatataaata gaatagaaac tagctaactc ccagtcaatt
    28321 ctgggagttt tatgcgtaat tgggcaagct ttctaggttg tctatcttgc taaaaacaag
    28381 aaaaaacggt atgatagaag agtaacaaca gaggtaacag agatgaacga gacacagatt
    28441 caaagagaaa cacgtcaggt agtggaagat gttttagaaa agaccaattt gaagcagggg
    28501 gccctttttg ttttaggtct atcttctagt gaggtgctag gtggtcagat tgggaaagaa
    28561 tccagtcaag agattggtga gctgatagtg gagactatct tgggtattct aggtagtaga
    28621 ggtatccatt tagcagttca aggttgtgag catgtcaatc gggctctcgt tgttgaacgt
    28681 caggtggcag agcagtttgg tctggaaatt gtcagtgtcc atcctactct tcatgcagga
    28741 ggttcggggc agttggcagc ctttaagttt atgcaggatc cagttgaggt tgaatttatc
    28801 aaggctcatg ctggattgga tatcggagac actgcaattg gcatgcatgt caagcatgtt
    28861 caggttccga ttcgccctat tttgagagag attggtcatg cccatgtaac ggcactggct
    28921 agtcgtccaa aattaatcgg aggtgcgcgt gcgcactatc cgcaagatgc tattaggaag
    28981 tcttgagaat gaataaagga gataaaagtg tttaaaaaag accgtttttc aattcgtaag
    29041 attaagggag ttgtaggctc tgtatttctt ggaagccttt tgatggctcc ttctgtagtg
    29101 gacgcagcca cctatcacta tgtaaataaa gagattattt cacaagaagc taaagattta
    29161 attcagacag gaaagcctga caggaatgaa gttgtatatg gtttggtgta tcaaaaagat
    29221 cagttgcctc aaacagggac agaagcatct gttttgacag cttttggttt gctgactgtt
    29281 gggagcttgc ttttaatcta caagagaaag aaaattgcta gcgtctttct agttggagct
    29341 atgggattgg tagttcttcc tagtgcaggg gctgtagacc cagttgcgac cctagcactg
    29401 gctagtcgag agggtgttgt tgaaatggag ggctatcgct atgttggtta tctatcaggt
    29461 gacatcctca aaacgcttgg cttggacact gttttagaag aaacctcagc aaaacctgga
    29521 gaggtgactg tagtcgaagt tgagactcct caatcaacaa caaatcagga gcaagctagg
    29581 acagaaaacc aagtagtaga gacagaggaa gctccaaaag aagaagcacc taaaacagaa
    29641 gaaagtccaa aggaagaacc aaaatcggag gtaaaaccta ctgacgacac ccttcctaaa
    29701 gtagaagagg ggaaagaaga ttcagcagaa ccagctccag ttgaagaagt aggtggagaa
    29761 gttgagtcaa aatcagagga aaaagtagca gttaagccag aaagtcaact atcagacaaa
    29821 tcagctgagg aatcaaaagt tgaacaagta ggtgaaccag tcgagccaag tgaagacgaa
    29881 caggcaccaa cggcaccagt tgagccagaa aagcaaccag aagctcctga agaagagaag
    29941 gctgtagagg aaacaccgaa accagaagat aaaataaagg gtattggtac taaagaacca
    30001 gttgataaaa gtgagttaaa taatcaaatt gataaagcta gttcagtttc tcctactgat
    30061 tattctacag caagttacaa tgatcttgga cctgttttag aaactgcaaa aggtgtctat
    30121 gcttcagagc ctgtaaaaca gcctgaggta aatagtgaga caaataaact taaaacggct
    30181 attgacgctc taaacgttga taaaagtgaa ttacaggaac aattaagagt tgctgaacaa
    30241 aaacaacaag ctgactatag cgctaaaact tggagggaat tcaaaattgc tgaacttcaa
    30301 gctaaagaaa tcaacaaccc aactacacca cttcctaaac aaagtgaaat agatgcagct
    30361 actaaggctc ttcaagacgc tcttcaagct cttgctgtag ataaaactgt attacaaaac
    30421 gctattaaca cagctaatag caaacgtgaa gaagagtata ctgctcagac atggaaagcg
    30481 ttaaaagatg ctcttacagc agtgaatcct gttaatgaag atgaaactgc aactcaaagc
    30541 aaagtagacg aagctactag aaacttagaa gaagctatca ataacctagt tctattaact
    30601 gaaaaaccgg tattaacatt tattgagact gataagaaag cattagaacg tgaagtagtt
    30661 gctaagtatt ctctagaaaa ccaaaataaa acaaaaatta aatcaatcac agctactctg
    30721 aaaaaaggag aaacagttgt tagcactgta gagcttatag gcgatgatgt aactaatgaa
    30781 actataacct ctgcatttaa gaatctagag tactacaaag aatacaccct atctacaact
    30841 atggtttacg atagaggtga cggtgatgta acagaaatct tagataatca accaatccaa
    30901 ctagatctta aaaaagttga gcttaaaaat attaaacgta cagatttaat caaatacgaa
    30961 aatggaaaag aaactaatga atcactaata acaactgttc ctgatgataa gcggaattat
    31021 tatttaaaaa taacttcaaa gaatcaaaaa actacattac tagctgttaa aaatatagaa
    31081 gaaactacgg ttaacggaac acctgtatat aaagttacag caatcgcaga caatttagtc
    31141 tctagaactg ctgataataa atttgaagaa gaatacgttc actatattga aaaacctaaa
    31201 gtccacgaag ataatgtata ttataacttc aaagaattag tggaggctat tcaaaacgat
    31261 ccttcaaaag aatatcgtct gggacaatca atgagcgcta gaaatgttgt tcctaatgga
    31321 aaatcatata tcactaaaga atttacagga aaacttttaa gttctgaagg aaaacaattt
    31381 gctatcactg agttggaaca tccattattt aatgtgataa caaacgcaac gataaataat
    31441 gtgaattttg aaaatgtaga gatagaacgt tctggtcaag ataatattgc atcattagcc
    31501 aatactatga aaggttcttc agttattaca aatgtcaaaa ttacaggcac actttcaggt
    31561 cgtaataatg ttgctggatt tgtaaataat atgaatgatg gaactcgtat tgaaaatgtt
    31621 gctttctttg gcaaactaca ctctacaagt ggaaatggct ctcatacagg gggaattgca
    31681 ggtacaaact atagaggaat tgttagaaaa gcatatgttg atgctactat tacaggaaac
    31741 aaaacacgcg ccagcttgtt agttcctaaa gtagattatg gattaactct agaccatctt
    31801 attggtacaa aagctctcct aactgagtcg gttgtaaaag gtaaaataga tgtttcaaat
    31861 ccagtagaag ttggagcaat agcaagtaag acttggcctg taggtacggt aagtaattct
    31921 gtcagctatg ctaagattat ccgtggagag gagttattcg gctctaacga cgttgatgat
    31981 tctgattatg ctagtgctca tataaaagat ttatatgcgg tagagggata ttcgtcaggt
    32041 aatagatcat ttaggaaatc taaaacattt actaaattaa ctaaagaaca agctgatgct
//
LOCUS       DAVLYV010000014        31858 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLYV010000014 DAVLYV010000000
VERSION     DAVLYV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31858
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.014"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(28..1455)
                     /locus_tag="VYO70_000676"
     CDS             complement(28..1455)
                     /locus_tag="VYO70_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1712945.1"
                     /translation="MKTTNKVAIYVRVSTTSQVEEGYSIDEQKDKLEAYCKIKDWKIY
                     DVYVDGGFSGANTQRPELERLISDVKRKKVDIVLVYKLDRLSRSQKDTLFLIEDVFAK
                     NDVAFISLQENFDTSTPFGKASIGMLSVFAQLEREQIKERMMLGKEGRAKNGKSMSWT
                     TIPFGYDYSKETGVLSVNPTQALIVNRIFTEYLNGKPVVKIIRDLNAEGHVGRKRPWG
                     ETITKYLLKNETYLGKVKYKDKVYEGQHEPIITQELFDLVQLEVERRQISAYEKYNNP
                     RPFRAKYMLSGLMKCGYCGASLGLRYTRKDKNGISHHKYQCRNRHSKDLEKRCESGWY
                     SKEELERGVIKELERIKFDPKYKNETLAKKEETIKVEEIKKQLERINNQVSKLTELYL
                     DEIITRKELDEKNDKIKTERQFLEEQLENQKSNVLSIRKRKLTRLLKDFDVEKLSYED
                     ASKIVKNIIKEIIVTKDGMSITLDF"
     gene            complement(1623..1823)
                     /locus_tag="VYO70_000677"
     CDS             complement(1623..1823)
                     /locus_tag="VYO70_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712946.1"
                     /translation="MSNESRPMEVIKHNLDCKCHRRREWIRVNDKWHAIEFSVDDPNE
                     PPMTEEEKANVALILQQHLSKE"
     gene            complement(1825..2616)
                     /locus_tag="VYO70_000678"
     CDS             complement(1825..2616)
                     /locus_tag="VYO70_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HEV1712947.1"
                     /translation="MARGRGKLTPQDKEDMKVFSANLNSILSDRNCKQAELSRATGIP
                     PSTLTGYVKGTSLPIPGNVQKIADFFGVPKSVLDPRFITNNFMVNDSSSNTPQIQTIY
                     DELEPPRQGKVLNYAKRQLKEQKNEEETKINEVSEAIRLYSYDYYDHPASAGTGQYLN
                     DVRVERIELPVDVDADFVIPIKGDSMEPDYHDGDLVFIQTSVDLNDGVIGVFNYNGDA
                     YIKQLVIDEDQAYLHSLNPEYKDMPITPDTDFRIIGEVVDLYREK"
     gene            2789..3064
                     /locus_tag="VYO70_000679"
     CDS             2789..3064
                     /locus_tag="VYO70_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712948.1"
                     /translation="MNTLNEKAINIFKAVAKETLIQGTYEENFLYSQLEAFCTNCRQF
                     AFGWTELAEEIERQERYLLDSGFTQEEIDDIRFDAAFAGMLDKLNVA"
     gene            3139..3306
                     /locus_tag="VYO70_000680"
     CDS             3139..3306
                     /locus_tag="VYO70_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712949.1"
                     /translation="MNESFLTILGISMIASFITNLIAYLAGKHHLKKKIKNHKMWFDS
                     EIERIKKKYHL"
     gene            complement(3297..3746)
                     /locus_tag="VYO70_000681"
     CDS             complement(3297..3746)
                     /locus_tag="VYO70_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712950.1"
                     /translation="MNNFSFIETLAIAAIPAFVSGMWSYIAAKGNSKHEIDKINIAHS
                     QELENVENQFKQDMEKMQKQHSQELYSLQQTHELRLLELEKVSQLDTQTDQGLKINDL
                     IFKAISGEISADVAIKNMSTLSHFANKQQPSDLQKQFVKKLSKKNHK"
     gene            3945..4115
                     /locus_tag="VYO70_000682"
     CDS             3945..4115
                     /locus_tag="VYO70_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712951.1"
                     /translation="MKEKLNEFLKFRSQFTKREWFEINQAVEACLNEKADQLKLDDSD
                     VEIISKRLGRSI"
     gene            complement(4340..4546)
                     /locus_tag="VYO70_000683"
     CDS             complement(4340..4546)
                     /locus_tag="VYO70_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712952.1"
                     /translation="MTDYQLEASLIVLGKEFDRTKKNGKESFSVHVSFFDGLDANQHL
                     QEFARQYPVKIDRSNSDQITFLIK"
     gene            4619..4846
                     /locus_tag="VYO70_000684"
     CDS             4619..4846
                     /locus_tag="VYO70_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1712953.1"
                     /translation="MPKMTLRAIRTNYNLSAKEVADKLNIHQQTLLKYEHDSSKIPMD
                     LLDKLARLYNVEKDFIFLGKKYELNHNLGEV"
     gene            4889..5251
                     /locus_tag="VYO70_000685"
     CDS             4889..5251
                     /locus_tag="VYO70_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1712954.1"
                     /translation="MQYIIRNTHGNYTSLNNAYAQDKRLKATTIGILTVILMNKSDWV
                     VYPDEIARRLGISRRTVDEHFKLLEKAGYLRVYRLGLGRGKGVTVYRFFSDMPISDDY
                     FEYLKTNLEKELSTDNGV"
     gene            complement(5646..5837)
                     /locus_tag="VYO70_000686"
     CDS             complement(5646..5837)
                     /locus_tag="VYO70_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000834563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712955.1"
                     /translation="MKSFKDFRESLTAEDMQAISAKANEATKQIDHTDGLQLGKVSGL
                     TSVITTIELLEKYHEWLHS"
     gene            5956..6099
                     /locus_tag="VYO70_000687"
     CDS             5956..6099
                     /locus_tag="VYO70_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712956.1"
                     /translation="MEDKIIELADYFISESTTYREAKIACEKLLKQVSHEIELRAMES
                     KTV"
     gene            6166..6963
                     /locus_tag="VYO70_000688"
     CDS             6166..6963
                     /locus_tag="VYO70_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEV1712957.1"
                     /translation="MNMNLNTNEILTTTEYDMFRKISNRKITENPKLEEELLSEGQRQ
                     PILVNEKMEVIDGQHRLYYLRKHRKPVRYIIDPTANFKTVISMNTSAVNWALQDYVYS
                     FALEGDPEFVKLAKFLDENELLSDKMVIVAGSGRRDGTAAHVVKKLKKGDYVFSNEKQ
                     LREFCKFYERVLNETKLPNKPFLQSVLWTLYTTSVFDENRMLTQLKKSDLTSEDIEGF
                     AKKKLLLTFLELYNGRWSDDHPSLIQYFINRKGSLTIPSLPKQDEDN"
     gene            6981..7118
                     /locus_tag="VYO70_000689"
     CDS             6981..7118
                     /locus_tag="VYO70_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000869366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712958.1"
                     /translation="MKVTVYVYGRKLEPDEPIIIPENHRFYDIWNGIVNEMLDNEEDV
                     A"
     gene            7118..7339
                     /locus_tag="VYO70_000690"
     CDS             7118..7339
                     /locus_tag="VYO70_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712959.1"
                     /translation="MKLLTKLKLRLEGVLKSVNLDWREVAVELMNDLFEERKRRFAFE
                     QENYDLKQELAAYKYKENFDIKARLQGEM"
     gene            7340..7594
                     /locus_tag="VYO70_000691"
     CDS             7340..7594
                     /locus_tag="VYO70_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712960.1"
                     /translation="MYIISIYVKNTETGNEDFSLIGRDFLPTGHQDYIARVFGTKEEA
                     IDYLKSISYIASGVHGNDWVYQNEKLPEIESRCRIWKVGE"
     gene            7607..7867
                     /locus_tag="VYO70_000692"
     CDS             7607..7867
                     /locus_tag="VYO70_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712961.1"
                     /translation="MFKALKTIKKIKQLQKEMHAFSLAFLALQDMGLMPETERSKAKA
                     QTMHDVSRVLKDVLDGKSVDEAIKRLNSEVKAEEVGQKDDQN"
     gene            8137..8955
                     /gene="bet"
                     /locus_tag="VYO70_000693"
     CDS             8137..8955
                     /gene="bet"
                     /locus_tag="VYO70_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HEV1712962.1"
                     /translation="MTNNQIVEAKGDFLTNPQLLNSGIIRKYLDPQGKASDEELAYFI
                     AQAKAQNLNPFTKEIYFIKYGTQPAQIVTAKSAFEKKADSHPQFDGKEAGVIYLMDGE
                     IKYSKGAFIPKGAEILGGWAKVYRKDRTYPTETEVSFEEYDNSKIRARVKELTQQGKD
                     VTYPVMNSYGKPIGENNWDTMPCVMIRKVALVSAYREAFPAELGASYEADEIQLDNTP
                     KDVTPQESREDVVARKMAQIDQFNKEQEANHADPEPAKTEEPIQGELLDGELEY"
     gene            8965..10029
                     /locus_tag="VYO70_000694"
     CDS             8965..10029
                     /locus_tag="VYO70_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1351 domain-containing protein"
                     /protein_id="HEV1712963.1"
                     /translation="MQELQVIDDKKINKIYEMITTDELTRESFEKDLIEATEKYKDYI
                     PTAGTLKDDKAKRAEFNKLIESKNRIRIDTKNLLSETANTWDSYAKSIIDPFATVVSE
                     FDKGIKEIEEHQRQLKIDTVKSYLANKSAEYMLDPRLFDEKALEYVKASDFMADGVTL
                     KKATMKSLDDMVTFEFQKQQEFEKAKSAISGLCAEYGMTDSPYIRQLKDLTLAEVFGQ
                     IKADYEFEKQKEELRQDLERAERSNQELLAAQQTKQQEQAPKSTETPNFDPETGKILD
                     GGQILQNEPNALRGAENDLKRYAQKMTLEVYFVDTAEKDRFKAGLSQLGFDFKKNYQV
                     RGYQRIDPLTQAELNEQCGW"
     gene            10197..10520
                     /locus_tag="VYO70_000695"
     CDS             10197..10520
                     /locus_tag="VYO70_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712964.1"
                     /translation="MDIRKISDSVAIYSDGKKLQVIHNLGDEFILDFKVGEDSVWNLN
                     GQVVEIIDMIEPVFKVFSFCSKSGEGMQRLKHAIVHFEKFEQYIRDNQEDLMIWWHNP
                     GGEYD"
     gene            10513..10848
                     /locus_tag="VYO70_000696"
     CDS             10513..10848
                     /locus_tag="VYO70_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein Cse60"
                     /protein_id="HEV1712965.1"
                     /translation="MIKTVFFSCDYPHHEVIDDQINSWLAENPGIKLIDIKFQSNVSA
                     VADSGVSAEYWHTSALIIYKVPSENNISSINSNGLGFIISCEKCGSLSIIKGKDVGQN
                     VCYECKGEK"
     gene            10943..11359
                     /gene="ssb"
                     /locus_tag="VYO70_000697"
     CDS             10943..11359
                     /gene="ssb"
                     /locus_tag="VYO70_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1712966.1"
                     /translation="MINNVTLVGRLVAPPDLRKTPNNVSSLQGTLAVNRNFKNENGER
                     EADFINFQAWRGTADIIAQYCSKGSLIGIIGRLQVRSYEKDGQRRYVTEVIAESVALL
                     ESRNSQHGQGNSFQNGNSSPFTDPNPFDLPNDGLPF"
     gene            11372..11770
                     /locus_tag="VYO70_000698"
     CDS             11372..11770
                     /locus_tag="VYO70_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712967.1"
                     /translation="MEIDKIIKKDVLEFMETISDNKIDASKINVTERIAIIIEKQKIE
                     VVTTLNYDMSISFDNKDTAPTLDDNGDLFEPVYKCKVKAIPKNDVFFTSLTRVKSNTK
                     TLQEVKKFFEFVNENRENLFEMAGFKGVLE"
     gene            11767..12198
                     /locus_tag="VYO70_000699"
     CDS             11767..12198
                     /locus_tag="VYO70_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HEV1712968.1"
                     /translation="MKLTLNIEPKPQSRPRFARRGSFTTTYEDKDMKTWRNHCQLLIA
                     NQYMGQPILEGALRTRLRFYIKPPQYISKVKKNQQALLDEIIPVGKKPDIDNYEKALY
                     DSMSGIVFQDDGQIALHDVGKFYSLNPRIEVEIEVMKSLSI"
     gene            12214..12396
                     /locus_tag="VYO70_000700"
     CDS             12214..12396
                     /locus_tag="VYO70_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712969.1"
                     /translation="MADYALYQGDVFVTLGTLAQISSETGITERMLKYYTYTSHQRRH
                     PNGRAVIKIEEEDNEN"
     gene            12421..12738
                     /locus_tag="VYO70_000701"
     CDS             12421..12738
                     /locus_tag="VYO70_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712970.1"
                     /translation="MMEELKQKVNEVYNWTVEDGKPQPPKQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPIDYDLGATKDWLPKSKEFDDWVGYSPSMAQVVIAVYLIY
                     RGN"
     gene            12740..13432
                     /locus_tag="VYO70_000702"
     CDS             12740..13432
                     /locus_tag="VYO70_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEV1712971.1"
                     /translation="MNKQELIKKLEERRTITGNFQGYVVWWKDVKEIFEQLDESEIGH
                     ADEAPRYVKNILARLRELPLHDREVWLKAIMSEFEQDFSRAKWREGYEQGKIEGMVER
                     EKVIVPQCVAEYIEFKKKNNFHVYGAMRVIEDHYDKKVPDWFYENNIEKFCLAWLDGY
                     EVEKEKRYFVKIKGNIKENMLVYGELLKRYFFTKSFSLDDVIYSHTRKELEDAKFGWV
                     FDCPGIEIEEVE"
     gene            13590..13850
                     /locus_tag="VYO70_000703"
     CDS             13590..13850
                     /locus_tag="VYO70_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1372 family protein"
                     /protein_id="HEV1712972.1"
                     /translation="MKRFIAIWILLSAGLNIWQMDRIRDLEEKKPMVIYKADNAGVEI
                     FGKVVEKGRHGKLYTLTIRDYGVFVVTKDVYDKVKVGDEVLL"
     gene            13847..14269
                     /locus_tag="VYO70_000704"
     CDS             13847..14269
                     /locus_tag="VYO70_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712973.1"
                     /translation="MTETIKLPDYYEPDWKNARYGSLEELKELLLFKRIVKWDKDFLL
                     LEDGTKVTIEMSESDCCASAGGEFQDVSLDAVITNIEIGEPEEIPDHWGTGYKNKVTI
                     FHNQNPVAIANCEAEHNGYYYSVCSLVIGDIHFPVVNA"
     gene            14281..14718
                     /locus_tag="VYO70_000705"
     CDS             14281..14718
                     /locus_tag="VYO70_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712974.1"
                     /translation="MTPRYRAWIKTEKRMVFSDDILAIDYENKEIVTQQVYFENGLPD
                     DRDIYCYDFDEIELMQSTGLKDKNGKEIFEGDIVRTTRFLGRADEIGGFYEYDKEFIG
                     IVKQLEGSWVIDTGSDAVCLWTEIEENEIIGNIYENKEFGGRK"
     gene            14715..15086
                     /locus_tag="VYO70_000706"
     CDS             14715..15086
                     /locus_tag="VYO70_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712975.1"
                     /translation="MRPCKYPYSGRRKKQETPSPIFSARPIFDEVPIVEEVKVEFGVE
                     ASMGRIYPETLIHLDISGYGNRVHSVHRFPGILLSVGESIQLKMLFYKRLRNFTTDRF
                     LTFRESDWKFFIRDLVNEFKH"
     gene            15159..15563
                     /locus_tag="VYO70_000707"
     CDS             15159..15563
                     /locus_tag="VYO70_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1712976.1"
                     /translation="MNKAKELLKELQDLDMDIQSRIDEINELEAGLLSSPKWSEVKVQ
                     GGQTRKVDDVYTQLVVMKEAIEQDTKEVINRKLELGRMINRLKNPKYRAVLRMTYINK
                     GTADSVCYDLNMSRTTYYRLKNEAVLALEEVI"
     gene            15644..15726
                     /locus_tag="VYO70_000708"
     tRNA            15644..15726
                     /locus_tag="VYO70_000708"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15675..15677,aa:Tyr,seq:gta)
     gene            15993..16472
                     /locus_tag="VYO70_000709"
     CDS             15993..16472
                     /locus_tag="VYO70_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEV1712977.1"
                     /translation="MAFFDYSKGGDGKLSGLRIKQKRFADEYIISGNATEAYKKAGYR
                     VSSDRVAGVEGHKLLKNPKIKSYIDERLKQLDSEKIADQQEVLGYLTSVMRGETQEQT
                     LISIGELGQTITDIDVGAKDRIKAAELLGKRHRLWTDKVEADVSGTVVFANESDISD"
     gene            16450..17748
                     /locus_tag="VYO70_000710"
     CDS             16450..17748
                     /locus_tag="VYO70_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEV1712978.1"
                     /translation="MSQTYQIKQNDIVVDLPKTVGAGYGQFWRSRSLYRVVKGSRGSK
                     KSKTTALNYVIRLLKYPWANLLVIRRYSNTNKQSTYTDFKWACNVLGVTHLFKFNESL
                     PEITIKATGQKILFRGLDDELKITSITVDIGSLCWAWFEEAYQIETEDKFSTVVESIR
                     GSLDVPDFFKQITVTFNPWNERHWLKRVFFDEETRRADTFATTTTYKCNEWLDEVDIK
                     RYEDLYHTNPRRARIVCDGEWGVAEGLIYENVTVKDFNKDELLQDSANKLCIGLDFGF
                     THDPTALCCSLINDTTKEIYVFDEAYKVGLITKEVAKMIKDKGYHRSQIIADSAESRL
                     IEELRSEHGISRIKESRKGKDSIMAGVSKLQGYAIYVHPDCKNIMDEFYSYCYQRDKE
                     GNWLNKPEDKNNHLMDALRYSLQCIEGGKATVRRRSDYGL"
     gene            17760..19229
                     /locus_tag="VYO70_000711"
     CDS             17760..19229
                     /locus_tag="VYO70_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1712979.1"
                     /translation="MYQYLTYPRDGYDEGSLKKDLIYKLITIHNTESSRLKNLKSYYM
                     GDHAILNHKRRNVNAPNYKTVANHAKDIADTATGYFMGNPIKYNNTADGDIDELLTAF
                     DGAEIDQVDAQNALNMAIYGRAYEYIYAKEGMTELDSTSIDPENTFMVYDDSIERKPL
                     FAVYYYEVKDDTKDTTKHQAEVFTENLHYHMVLRSTDSGTTQSEEATPHNLGQIPIIE
                     YRNNHFAIGDYEQQISLIDAYNSLMGNRVNDKEQAVESILVLYGTQLADTPEDAKVAM
                     KILSEEGLLELPGDSARAEFLKNTLDESATEILRTALKEDIYTFSHVPNLTDENFAGN
                     TSGVAMEFKLMGLEMITKTKEANYKRGLRQRIAIFAHYLGMKQIALESHSIVPQFSRG
                     LPKNLLEISQIVNNLEGKVTNRQLISLLPFVEDPDAELEALEEEKKKNMEDMPMFNQE
                     NTKPEDEVEDEESGVLGEEESQSDLPADGQGRKAGRPVR"
     gene            19138..20775
                     /locus_tag="VYO70_000712"
     CDS             19138..20775
                     /locus_tag="VYO70_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEV1712980.1"
                     /translation="MKNQEYWAKRKANLIYQQMDKAEKQADQFDKVYQEAKTYLDKEV
                     NKIFDKFQRDYGLSQVEARQVLKNMKDKKNLNELRKVLEARPNDPNIQRLLADLDSPA
                     YSFRMKRLERLSDDLDRMRESIYHSEKTGSDAFYSDLMKDSYYKATFDLQQQTGLAYG
                     FSGLPDSEIKHLQSFSWVGDGSTYSTNIWKNTGKLTSSIKDELLMSLMTGRDTRETAQ
                     AIAERFNVGQNDARRLVRTESAFFHNQMELLSYEEADIEKYIFVAVLDKRTSRICQEH
                     DNQVYDRDKAVPGVNCPPMHPWCRSTTVGYDEDADYSKLKRRARNPETGKVEYVPADM
                     TYKEWYSKYVAKDGEKVYNQDTREAKAKFYSEQLLSKISGVEPKITSDMQRIAGENKL
                     AGLEFRKKTVESLSRKIIADSLVENISLSKAVSKINDALRYTTIFDSDTFTEEYLKMK
                     QKLIAEGYKIVKVKNTWLVDGPYKGVNTVVEKDGINFEMQYHTQESFDLKNGSLHELY
                     EKYRDTNTSDLERMKLFKEMLDLSNGLEIPKNIERVK"
     gene            20777..20986
                     /locus_tag="VYO70_000713"
     CDS             20777..20986
                     /locus_tag="VYO70_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712981.1"
                     /translation="MKDIKYYRTTTNNAQVLRLIDGVMQVFDIEKKWVNSMDWFNKIF
                     FNDFTDFEEISENDAFTYIDRMVAA"
     gene            20983..21396
                     /locus_tag="VYO70_000714"
     CDS             20983..21396
                     /locus_tag="VYO70_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1712982.1"
                     /translation="MIDIALAIAKKAHAGQVDKAGVDYIQHPLYVASQVNTEQEKAVA
                     LLHDVIEDSDITAADLFASGLSNEVVTAVQILTKKKGQSYQEYLGKVKSNNLARVVKL
                     ADLKHNSDLSRLKSVTNTDYERVKKYKNAIYYLST"
     gene            21439..21726
                     /locus_tag="VYO70_000715"
     CDS             21439..21726
                     /locus_tag="VYO70_000715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712983.1"
                     /translation="MNKYKKLIELIENNGLEIQSKKCYDPQSAWHGEELWIVDKKKQN
                     KIFDLSGNGYCFHDAKVEEAIEEVEKYLLLKKMDTFDDFKKWVEKNAKPKK"
     gene            21867..22079
                     /locus_tag="VYO70_000716"
     CDS             21867..22079
                     /locus_tag="VYO70_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712984.1"
                     /translation="MEEWKERFKKEYYELKERFQKLDMMIGKYEKGQLEFESKCPIDL
                     LKGQRSTMWNYLRILEQRAKIEEIKL"
     gene            22249..22773
                     /locus_tag="VYO70_000717"
     CDS             22249..22773
                     /locus_tag="VYO70_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEV1712985.1"
                     /translation="MSEEINATVSTESTETVDTQENVDTVQEEKHERTFTRAEIGKML
                     SAERSKWEAEQEAKENEAKKLAKMNADEKQKYQLDQREQELADREKAIARKELTAEAK
                     AMLSERDLPVELVNVVDLTNAETVSESITSIQKAWEESVQKGVSERMKGSAPIKNAQT
                     VQQEVTEKWRKDFL"
     gene            22796..23821
                     /locus_tag="VYO70_000718"
     CDS             22796..23821
                     /locus_tag="VYO70_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEV1712986.1"
                     /translation="MAFEELNTAESRKKHLGIIEDVLAVNSYSTPLVTSSDAVTLQGR
                     SFTVATGNTTELKDYKRNKDNEFDHVEVEEKVYTLDEEKYWGRFVDQLDERDSNGQVN
                     INYVIARQAAEVVAPYLDELRFGAALGNVSDNVAMGKTAGANNAYNAVLDVSEKLDEL
                     GITKERLLFVTPSFYKAIKSEIVRLPHGDADKKVLGKGYVGELDDYTVYKVPSKFLKG
                     VNALATAPGVVTSPVQVDNTKYNDNIPGRFGELVEQLLYTGAFVLEHFKKYIITIADS
                     KPAAKKSVQGKVVNRAKAWKTGTTYKEGESVTHADKVYVAVKDISNSATAPDTDTTNW
                     KEKIGKK"
     gene            23831..24019
                     /locus_tag="VYO70_000719"
     CDS             23831..24019
                     /locus_tag="VYO70_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712987.1"
                     /translation="MKFKIKQDFYDWESNVKRLVGEELEITEERYAELADNFASNGVA
                     ISDVLEETLPEPEFLEED"
     gene            24021..24332
                     /locus_tag="VYO70_000720"
     CDS             24021..24332
                     /locus_tag="VYO70_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEV1712988.1"
                     /translation="MSIELLKKMTGEEDTQLLMLLQTRATNLILSETNRTSLTPALSL
                     LIPEVAIELHNRSGVEGEHSRTEGGIAVVYGENGLSTGLLQRIRMHRLARVAGHVFEA
                     E"
     gene            24313..24651
                     /locus_tag="VYO70_000721"
     CDS             24313..24651
                     /locus_tag="VYO70_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712989.1"
                     /translation="MFLKQSRLKPYPMRRFEKTVTEEGVAKEGYVKEAETVRLELWPA
                     SSKLQSELYGERVNDILNANANKSATIKVKDGVCIDSQTEVTHKVISKKVYTHHQVLE
                     LERVRATRGR"
     gene            24626..25141
                     /locus_tag="VYO70_000722"
     CDS             24626..25141
                     /locus_tag="VYO70_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1712990.1"
                     /translation="MSELLGADRLIAKCRRLASKKTGEDIVLRAVHNATIKVVQADAR
                     RLAPARDGELIISIKTRAKMDGDRAIGEVYTNLKYAPYVEFGTGPIGQANHSGISPEV
                     SVTYKSNPWYVHEDQINVGPYHFQKIGEFYKMYGQPAQPYLYPALRDNQERVSKNISN
                     YVRRKIREQIK"
     gene            25138..25506
                     /locus_tag="VYO70_000723"
     CDS             25138..25506
                     /locus_tag="VYO70_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712991.1"
                     /translation="MINIKPVIYKELQKVADNVTDTYPSDWETFPVVIFLEEQNKPGD
                     WFDDQEQKSSIRYKVDIFDDTSTSELAVKINKIFESLGLRRTDCQDVPDPSHLRHKVM
                     RFEGVVDLDSELVFQFRMEN"
     gene            25509..25922
                     /locus_tag="VYO70_000724"
     CDS             25509..25922
                     /locus_tag="VYO70_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000880678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1712992.1"
                     /translation="MLANGIKLAFSETKGNYQNLVGLKEVPEFGIEPEKVENTTLADT
                     VKKYEFGIGDAGELEYKFAYNNSSATAPYRVLRKAADGKKKLYFEQTYPDGTKVTFEG
                     QVSVKLGGGGVNAVIEFTLKIALQSNLTFTDGVGG"
     gene            25927..26307
                     /locus_tag="VYO70_000725"
     CDS             25927..26307
                     /locus_tag="VYO70_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712993.1"
                     /translation="MALKYTTWKVTDEKELKLRLTSHQAATVEEKIGMNLLKIFMPEA
                     GEEFTLPPLKVMLLLVHGALQQYEHGYSFEDVYDLYDEYVDNGGDQTTFMTEVLMPLF
                     EVSGFTPRGSKDKKTSKKKMTVVE"
     gene            26346..26696
                     /locus_tag="VYO70_000726"
     CDS             26346..26696
                     /locus_tag="VYO70_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000478007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712994.1"
                     /translation="MFLDIGGKPLDFWDLTVLEIREMIESYNRVKIQERKEKIIDSYR
                     LSQMISNHVSLLLSKDAKAFEFWEYAPELFVEEQQAVEQERQRQALLLHKERMRDFAE
                     RHNRKRKEEVNGNS"
     gene            26683..30186
                     /locus_tag="VYO70_000727"
     CDS             26683..30186
                     /locus_tag="VYO70_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712995.1"
                     /translation="MATLDELKVMIDAEIAPFRKKMKEVENQVKGTSDQVKNATAKVR
                     EQSNSIGSAFGKLAKFAGFAILGKKLLDVGMYSTQTALEVSASMNQIKRQMGESSQSF
                     LKWVNDNANAMNMGVGEATNYGAVYSNLFSGFIKDTNKLSAYTAKMLQTSAVVAEGSG
                     RTITDVMERIRSGLLGNTEAIEDLGINVNVAMIESTEAFKKFANGQSWQQLDYQTQQQ
                     IRLMAILEQATAKYGNTLSNSVNGRISLFKSLMKDAALNLGNSMLPIINAIMPVLNSF
                     AMVLKNVTAKLAEFIALMFNKKATVKDGVGGAVGDMGNAMKDAAGGAGDLADAVDDAG
                     DSAGGLADNLGDSAKNAKKAAKELLGLLGFDEINILQKPKDDDAGGSGGGGGPFKDIL
                     PEVELTDMDNKFKSIFDGLGDKLKGLFDLFKKGFDAAFRPEGIKRIKTALDQIAKTMG
                     EIATDPRVVNAFNRMAEKIAYALGQVTGSITTIGLGIGVFLAESIANGLGRQKERIIR
                     ALVALFDNVGNLSEAVGNIAQDFSSAFYDVITSTGAVRIGSAIVSTLLSLTSTIVEVG
                     SKLAGSLFKGFEKVVVTSAPKISSVFQSLLDTVAPVFENIERSVNKFGDGLSRVYDEH
                     VVPAINSIANAFNGLIDIIQILWENSWQPFAEFLSGVFGVSIEGISDLLGGGLLATLG
                     LLADAIKLVADGFTVFSDWCKENKEPIVALITTWQTINFLSWAEQAGGLAGAFSLLGS
                     KVSLIVGGIKNLGLAIKALTFDKLVSFGETIYLNTLYAKDFVVNSGKTIAQLGKTALE
                     LGKSALAWTAHAAKMGLATAAEFAHSVAAGVATAATWAFNAALAVLTSPITWIIAAIA
                     ALIAIGVLLYQNWDTVVEFAKTAWQGLCDFISGICQAIGEFFSGLWTKLQEIFEPIGQ
                     WFGEKFQQAWDAIVNIFSGIGEWFSGVFQGAWDAIVNIFTPIGSWFGQRWADVTNALS
                     SVSNWFGEMFTNAYNAVKDAFSSIGDFFKGVWDTVKSIFVNAGQMVGEAVGGAFKSAV
                     NAVLGTIENVVNGFIGMINGVLGVVRNLPGLGWVGSVSTVSLPRLARGGIVDSPTIAM
                     IGEAGKEAVVPLENTGFIQTLGRVVSSAVVNAMAGVSPQGGFSGDGDIVIQIAGHEFG
                     RVAIQEINKEHERAGQTLLKI"
     gene            30195..30545
                     /locus_tag="VYO70_000728"
     CDS             30195..30545
                     /locus_tag="VYO70_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1712996.1"
                     /translation="MAQLTINGVAVKPPKSFQVGIQDIDGETGRNANGDMVRDRITTK
                     RKLDCEWGMMTQGEISQLLHAVSSKFFEVSYPDPMDGQVTKTFYVGDRTAPSYTFTEK
                     FKPWSGAKFNLVER"
     gene            30550..>31858
                     /locus_tag="VYO70_000729"
     CDS             30550..>31858
                     /locus_tag="VYO70_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1712997.1"
                     /translation="MDALTRRQFDRAMFAKERTLAIRVGDYTSRDIKEASFEYGYIKG
                     DTYKPGGTCAGSGKITFTSIITTFNKLDTLHPEIGLLVGDTYQWVKMGEYFINDIEID
                     RNRNTTTLELMDGMFKLNREYVTDLHFPAEVREVIQEICLKTGIELANDYFGISAMRY
                     HIEQVPEGKKLSFRDMLSAMTQVIGMSCFFNREGKMEIRDLTESNITINADSYFLHGL
                     TKSEIEYQIAGITCKTDKKSLTVGMKTGRSLELDNVFMTQSALNDLYYKLKNLTYYPY
                     NLNYQGHLLLEVGQWVTIQTNKKETFKVPVLSQSFIFKGGLRGRISADSKAGNDTQYS
                     YEGTITKQIKQQDGFEAKIQAQIEAADKDFDQKVDKIKKDFNDQVELTKARAEEVKRE
                     LSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASTLLAQEAKRI"
BASE COUNT        10353 a         5501 c         7242 g         8762 t
ORIGIN      
        1 ctaaaataca gaaatacgct agacccttta aaaatctagc gttatcgaca tgccgtcttt
       61 tgtaacaata atttctttta tgatattttt gacaattttt gaagcatctt cgtagcttaa
      121 tttttcaaca tcgaagtcct ttagtagccg tgttagcttt ctctttcgga tgctcagaac
      181 attcgatttt tgattttcga gctgttcttc caggaattgc ctttctgttt tgattttatc
      241 atttttctcg tccaactctt tccgagtgat gatttcgtcc aagtacaatt ctgttaattt
      301 tgagacttga ttatttatcc gttcgagttg tttctttatc tcctcgacct tgatagtctc
      361 ttctttctta gcgagtgtct catttttata ttttggatca aatttaattc tttcaagttc
      421 cttgattaca cccctttcaa gttcctcttt agagtaccac cctgattcgc atcttttttc
      481 taaatctttg ctgtgtcggt tcctgcattg gtatttatga tgagagattc cattcttgtc
      541 ttttcttgta tatctcagac caagtgaagc accacaatat ccgcatttca ttaatccaga
      601 aagcatatac tttgccctga acggccttgg attgttatat ttttcatagg ctgaaatctg
      661 ccttctttcg acttcaagct ggactaagtc aaacaattct tgagtgataa ttggttcatg
      721 ctgcccctcg tataccttat ctttgtactt tacttttccc aaataggttt catttttcag
      781 caagtatttc gtgattgttt cgccccaagg tctttttctt ccaacgtgac cttcagcatt
      841 taagtctctg atgatcttga ctactggctt accgttcaag tattccgtga aaatacggtt
      901 gacaatgagc gcttgggtcg gattcactga taagacgcca gtttcttttg agtaatcata
      961 gccgaatggt attgtcgtcc atgacatgga ttttccattc tttgcccgtc cttcttttcc
     1021 taacatcata cgctctttaa tctgctcacg ctccagctga gcgaatactg atagcatacc
     1081 gattgaagcc ttgccgaaag gcgtagaagt gtcaaagttt tcttgcaagc tgataaatgc
     1141 cacatcgttt ttagcaaaca catcttcaat cagaaacaga gtatctttct ggctacgact
     1201 gaggcggtct agcttgtaga ctagcactat atcgactttc ttgcgcttca cgtctgagat
     1261 taaacgctcc aattcaggtc tttgagtgtt agcacctgag aaaccaccat caacgtagac
     1321 atcgtagatt ttccagtctt tgattttaca gtaggcttca agcttgtctt tctgctcatc
     1381 gatagagtaa ccctcttcca cctgcgaagt agtggacacc ctgacgtaga ttgcgacttt
     1441 attcgttgtt ttcattgaat ttgtacccct tttttgataa aataggtaca agaaaacgtc
     1501 tcaaaaggta atatcttttg aaaagttttt cttgccacta gcctcacgct cggagtcgcc
     1561 aaactttgag agcgtggggc tttttttgtt tgcaattatt tccattttgg aaacagttgg
     1621 ttttattctt tcgataagtg ttgttgaaga attaaggcca cgttggcttt ctcttcctct
     1681 gtcataggag gttcgtttgg atcgtctacc gaaaactcga tagcatgcca tttatcattt
     1741 actctaatcc actctcttcg tctgtggcat ttgcaatcta ggttgtgttt aatcacttcc
     1801 atcggtctac tttcgttact catgttattt ctccctatac aaatccacga cttcgccgat
     1861 aattcggaag tcggtgtctg gtgtaattgg catatcttta tattcagggt ttaggctatg
     1921 taggtaagcc tggtcttcat caataacaag ctgcttgata taagcatcgc cgttgtagtt
     1981 gaatactccg ataactccgt cattcaaatc tacacttgtc tgaatgaata ccaggtcacc
     2041 atcgtggtag tcaggctcca tggagtcacc tttgatgggg atgacaaagt cagcatcaac
     2101 atctactggt aactcaatcc gttccactcg tacatcattc aaatactggc ctgtacctgc
     2161 ggaagctggg tggtcgtagt agtcgtaact gtagagccga atagcttctg atacttcgtt
     2221 tatcttcgtt tcttcttcgt ttttttgctc tttcagttgc ctctttgcat aattcaggac
     2281 tttgccctgt ctaggtggtt ctagttcgtc gtagatggtt tgaatttggg gagtattaga
     2341 agaagagtca ttgaccatga aattattagt tataaatcta ggatctaata cagatttagg
     2401 aactccaaaa aaatctgcaa ttttttgaac attaccaggg attggcaaag aagttccttt
     2461 tacataccct gtcaatgtac taggcggtat tcctgttgct cgagacagct cagcttgttt
     2521 acaatttcta tcagataaaa ttgagttaag atttgcagaa aagactttca tatcctcttt
     2581 atcttgagga gttaatttcc ctcgtcctct tgccatattt ttacctccta tcttctttac
     2641 tatataatac cgtttatttt cgattttgta aataaaaaat tcgaaaaaat tacgaaaaaa
     2701 ttcgaaaaat cattgacata cgatttaaat cgtagtataa tataatcaag cttaaggaaa
     2761 taacaaaaac aaaccggagg gaaacaccat gaacacatta aacgagaaag caatcaacat
     2821 ctttaaagca gtagctaagg aaactttaat ccaaggcact tacgaggaaa acttccttta
     2881 cagccagctt gaagcattct gtactaactg ccgtcaattc gcttttggat ggacagagtt
     2941 agcagaggag attgaacgtc aagagcgtta ccttctcgat tctggtttca ctcaagagga
     3001 aatcgatgac attcgttttg atgcagcatt tgcaggaatg cttgataaat taaatgtagc
     3061 ctgattggta tcaccaaggt tcgaatcctt ggcaggttgt tgctcataga gcgaaaaaag
     3121 agagaaagga gtagaacgat gaatgaatct tttcttacga tactaggcat atcaatgatt
     3181 gctagtttta tcacgaattt aattgcttac ttagctggta agcatcatct gaaaaagaaa
     3241 attaaaaacc acaaaatgtg gtttgattct gagatagagc gtatcaagaa aaagtatcat
     3301 ttgtgatttt tcttggataa ttttttcaca aattgttttt gaagatcgga aggttgttgc
     3361 ttgttcgcga aatgactaag agtgctcata ttttttattg ctacatctgc agatatttct
     3421 cctgaaatag ctttaaaaat taggtcattt atctttaagc cttggtcggt ttgagtgtct
     3481 agttgagata ctttttcaag ttcaagtaat cttaattcgt gagtttgttg cagcgaataa
     3541 agttcttgtg agtgttgctt ttgcattttt tccatatctt gtttaaattg attttcgaca
     3601 ttttcaagct cttgtgagtg tgcaatgtta attttatcaa tttcatgctt gctgtttcct
     3661 ttagcagcga tgtaagacca catgccagaa acaaaggcag gtatggcagc gatcgcaagt
     3721 gtttctataa aactaaaatt attcataaga tttctccaat cattttattt tgattatacc
     3781 acatttgaaa ggtagttaga attggaagat aaaatcattg aacttgctga ttacttcatc
     3841 agcgaaaaca caacgtacag agaagctaaa atagcgtgtg agaagctatt tcttcaagtc
     3901 agtcatgaga tagaactcag ggcaatggaa agtaggacga gggtatgaaa gaaaaactaa
     3961 acgaatttct aaaattcaga agccaattta caaaacgaga atggtttgaa atcaaccaag
     4021 ctgtcgaagc ttgtttaaat gaaaaagccg accagttgaa actggacgac tcagatgtag
     4081 aaatcatttc taaaagacta ggacggtcta tctaaagaaa gggtcaaaca aatggcgtct
     4141 aacagaacta tatcagtaaa tacatcagag catgatgtat tgttgacggc aagaaaaaac
     4201 caccctgctg tattcgtcga tggaatgttt ctcgacggag ttgagcgagt ggaatttacc
     4261 aatcattatc tggagaagtg tgaagttgtt ttaacgttta acgatagagt tgaaaccaat
     4321 cccttccctc taaacgatat tactttatta gaaaagttat ttggtcagag ttcgaacggt
     4381 caatctttac gggatattgt cgtgcaaact cttgaagatg ctgattagca tctaaaccat
     4441 caaaaaaaga aacatgaaca ctaaaacttt cttttccgtt tttcttggtt ctatcaaatt
     4501 ctttgccaag gacaattaga gaagcttcta attgataatc agtcataaca tcacctcctt
     4561 tctagcttta ttatagcaga attgcgagga acaaatagaa aaataaggag gtaggaacgt
     4621 gccgaaaatg acattgagag caataagaac aaattataac ttatctgcaa aagaagttgc
     4681 cgataaactt aacattcatc aacaaacact gttgaagtat gagcatgata gttcaaaaat
     4741 tccaatggat cttttagata aacttgctcg actatacaat gttgaaaagg attttatttt
     4801 tttaggcaaa aaatacgaat taaatcataa tctaggagag gtgtgaatga acgagcgaga
     4861 gttatagaaa ggggattaaa tatgaggtat gcagtatata atcaggaata ctcatgggaa
     4921 ttacacatct ttgaataatg cttatgctca agacaaacgt ttaaaggcaa caacgatagg
     4981 tatccttaca gtaatcttga tgaataagtc tgattgggtt gtgtatcctg acgagattgc
     5041 acgacgtcta ggaataagca ggcgcaccgt agatgagcac tttaagcttt tagagaaagc
     5101 aggctatctc agagtatatc gcttagggtt aggcagaggt aaaggcgtaa cggtatatag
     5161 atttttttca gatatgccta tttcagatga ttactttgag tatctaaaaa ctaatcttga
     5221 gaaagagtta tccacagata acggagttta aaaatacagt tggaaaatat tgccatgtgt
     5281 aaaattgcca tgtgtaaaat tgccatgtgt aaaattgccc tctaataagt actaactata
     5341 caacaagtac taactataca ataatctaag cctaacggca ctaacttagt aataactact
     5401 aacttacaac aaactaatac ttctctaaaa aataaaagag agaaatttca attttaggac
     5461 tttgaaaaat aggaaaggag aaaaaatgag accaagacga tatccgtata gtgggaaaaa
     5521 agagtccacc tttgtaaagg ccgaccctga gttagttgaa aaacttttaa gaaacactag
     5581 ttatcttgag tgtttacaaa aaaagcctat caattttcag atagactcag aagaatttaa
     5641 gcgtcttagc tatgaagcca ttcatgatac ttctcaagta actcaatagt agttattaca
     5701 gaagtcaaac cactgacctt ccccagttgc aatccgtctg tatggtcaat ctgtttagta
     5761 gcttcattag ctttagcaga gatagcttgc atatcttcag ctgttaaaga ttctcgaaaa
     5821 tctttaaagg atttcataaa aactcctcct ttctattgga attttgacta aaacggtgag
     5881 aggtcctagt cgagattatt atagcaattt aggaggatat tacatcggtc ttgaggctga
     5941 tttttggagg caatattgga agataaaatc atagaacttg ctgactactt catcagcgaa
     6001 tctacaacgt acagagaagc taaaatagcg tgtgagaagc tattgaaaca agtcagccat
     6061 gagatagaac tcagggcaat ggaaagtaag acagtctaga agacaacaaa aagcacctga
     6121 cggcaatcag gcgcatacta aaaaacttta caagaggatt ataacatgaa tatgaactta
     6181 aatacaaatg aaattttaac aactactgaa tatgatatgt ttcgcaaaat tagtaataga
     6241 aaaataactg aaaatcctaa attggaagag gagctacttt ctgagggaca acgtcagccg
     6301 attttggtaa atgaaaaaat ggaagttatc gacgggcaac atcgtcttta ttacttgaga
     6361 aaacatagaa aaccagtgcg ctacataatt gatccgaccg ctaatttcaa gacagtaatt
     6421 tcgatgaata catcagctgt caactgggca ttacaagatt atgtgtattc gttcgcttta
     6481 gagggagatc ctgaatttgt taaattagct aaatttttag acgagaacga attgcttagt
     6541 gacaagatgg taatcgtagc tggttcagga agacgtgatg gtacggcagc acacgtagtc
     6601 aaaaaattaa aaaaaggcga ttatgtattt tcaaacgaaa aacagttaag agaattttgt
     6661 aagttctacg aacgcgtttt aaacgaaaca aaactgccta acaaaccatt tttacaatct
     6721 gttttatgga ctttgtatac aacatcagtt tttgatgaaa acagaatgtt gacacaattg
     6781 aagaaatcag atttgacatc agaagatatc gaaggatttg caaagaaaaa attgctatta
     6841 acttttttag agctatacaa cggaagatgg agtgacgatc atccttcttt aattcaatac
     6901 tttatcaaca gaaaagggtc gttaacaatt cctagtttgc ctaaacaaga tgaagataat
     6961 taaaaaagta ggaagataaa atgaaagtca cagtatatgt ttacggccga aaattagaac
     7021 cagatgaacc aattatcatc ccagaaaatc atcgttttta tgacatttgg aacggaattg
     7081 taaatgaaat gctcgacaac gaggaggatg tagcttaatg aaactactta ctaaattaaa
     7141 actcagactc gaaggagttc ttaaatcagt caaccttgac tggagagagg tagcagtcga
     7201 acttatgaac gacctatttg aggagcgcaa gcgtcgcttt gctttcgagc aagaaaacta
     7261 tgacttgaag caggagcttg ctgcctacaa gtacaaagaa aactttgata tcaaggctag
     7321 actgcaagga gaaatgtaga tgtacattat atcgatttat gtcaagaata ctgaaactgg
     7381 aaacgaggat ttcagtttga ttggacgtga tttcttaccg acggggcacc aagactatat
     7441 tgcaagagtt tttggaacaa aagaagaagc gattgattac ttaaaatcta tatcttacat
     7501 cgcatcaggt gttcatggta acgattgggt ttatcaaaat gaaaaactac cagaaattga
     7561 gtcacgttgc cgaatttgga aagtaggaga ataaaaggag aacaatatgt ttaaagcact
     7621 aaaaacaatc aaaaaaatca aacaacttca gaaagaaatg cacgctttca gccttgcgtt
     7681 tctagctcta caagatatgg gcttgatgcc agaaactgaa agaagtaagg caaaggctca
     7741 aacaatgcac gatgtaagcc gcgtactcaa ggacgtcttg gacggcaagt cggtagatga
     7801 agcgataaag cgtctaaata gcgaagtgaa agctgaagag gtggggcaga aagatgacca
     7861 gaattgaact tgaaaaccgt gtatggcttt tggctaatca tgaagaaaaa aacgaattgc
     7921 tggatcttgg gctaacgtca aagaccagat atgtgaagcg agttctggaa ctaggaaagg
     7981 tgtatgctca tgtttgatta tgacagagat ataatgcaac cgcctgaaga acgagaagaa
     8041 cgtgacccag ctgattggat tttcagcgct ggtcaatgga tctatgtagg agattgttag
     8101 cctatgaata gagaacacaa cgaaaggtag aaggaaatga cgaataatca aattgtagaa
     8161 gcaaaaggcg actttttaac caacccacag ctacttaata gcggtattat cagaaagtat
     8221 cttgacccac aaggaaaagc tagtgatgag gagcttgcct attttatagc tcaagccaaa
     8281 gcccaaaacc tcaatccatt tacaaaagaa atttatttta tcaagtatgg aactcagcca
     8341 gcccagatag tcactgccaa atcagctttt gaaaagaaag cagatagtca cccgcaattt
     8401 gatggcaaag aggcaggcgt aatctatctg atggatggtg aaattaaata ctcaaaagga
     8461 gcatttattc ctaaaggtgc tgaaattctt ggcggttggg ctaaggtgta ccgcaaagac
     8521 cgtacttacc caacggaaac agaagtatct tttgaggagt acgacaattc taaaatacgt
     8581 gcaagagtta aggaactgac acaacaaggt aaagatgtta cttatccagt gatgaactca
     8641 tacggtaagc caataggtga gaataattgg gatactatgc cttgtgtcat gatacgaaaa
     8701 gtagctctag tgtcagctta ccgtgaggcg tttcctgccg agcttggagc aagctatgag
     8761 gctgacgaaa ttcagctgga taacacacct aaagacgtca ctcctcaaga aagccgtgag
     8821 gatgtcgtag cacgcaagat ggctcagatt gatcaattca acaaagagca agaggcaaat
     8881 catgcagatc ctgaacctgc taaaactgag gagccaatcc agggcgagtt gctagacggt
     8941 gaacttgaat attaggagga caacatgcaa gaattacagg ttattgatga taagaaaatc
     9001 aataaaatct atgagatgat cacaacggat gaacttacta gagagtcttt tgaaaaagac
     9061 ctcatagagg ctactgagaa gtacaaggac tatattccta cagctggaac tctcaaagac
     9121 gacaaggcaa agcgggctga atttaacaag ttaattgagt ctaaaaatcg tatccgtatt
     9181 gacactaaaa acttactatc agagacagct aacacatggg atagttatgc taagtcaatt
     9241 attgacccat ttgcaaccgt agttagtgaa tttgacaaag gtatcaagga aatcgaagaa
     9301 catcaaagac aactaaaaat agatacggtt aagagttacc tagccaacaa atcagctgag
     9361 tacatgctgg atcctcgtct ctttgatgaa aaggcccttg agtatgtcaa agctagcgat
     9421 tttatggcag atggcgtgac gcttaaaaaa gccactatga agtcacttga tgacatggtc
     9481 acatttgaat ttcagaaaca acaagaattt gaaaaagcca agtcagctat ttcagggtta
     9541 tgtgctgagt acggcatgac tgactcacct tacattagac agctgaaaga cttgactctt
     9601 gctgaagtct ttggacaaat caaagctgat tatgagtttg aaaagcaaaa ggaagaactc
     9661 agacaggatc tagaacgagc agagcgatct aatcaggagc ttttagcagc ccaacaaacc
     9721 aaacagcaag aacaggctcc aaaatcaacg gaaaccccaa attttgaccc agaaacgggc
     9781 aaaatcttgg acggtggaca aatcctccaa aatgagccta acgctcttag aggggctgaa
     9841 aatgacctaa aacgatatgc ccaaaaaatg actttagagg tgtattttgt agacacagcc
     9901 gaaaaagacc gtttcaaggc tggtctaagt caactcggat ttgattttaa aaagaactat
     9961 caagtcaggg gttatcaacg tatcgatcca ttaactcagg ctgagctaaa tgaacaatgt
    10021 gggtggtaag tatgacagaa attgaaaaaa tttcagaaga attggctgaa tatggggtgc
    10081 ctgatgagtt aataggaaaa atagaggacc tattagcgac tctgtatggc gaaaaaagga
    10141 aattggagat agaaaaatct tgggatattt ctccagagtc aatggggagg gtaaccatgg
    10201 acatcagaaa aatatctgac agcgtagcca tctactcgga cggcaagaaa ttgcaggtta
    10261 tccacaacct aggggatgag tttatcctag attttaaggt gggagaggat agcgtctgga
    10321 acctcaatgg ccaagtcgta gaaattattg acatgattga gcctgtcttt aaagttttca
    10381 gcttttgctc aaaatctgga gagggtatgc aacgcttaaa acatgctatc gttcactttg
    10441 aaaaatttga gcagtacatc agagataatc aggaagacct gatgatctgg tggcacaatc
    10501 caggagggga atatgattaa aaccgtattt ttttcatgtg attatccaca tcatgaggtg
    10561 attgacgacc aaataaatag ttggcttgcc gaaaatccag gcattaagtt gattgacatc
    10621 aaatttcaat caaatgtgtc tgctgtcgct gacagtggag tcagtgctga atattggcat
    10681 acatccgcat tgattattta caaagttccc tcagagaaca atataagcag tattaattca
    10741 aatggtttag gtttcataat cagctgtgag aaatgtggta gcttatcaat aatcaaggga
    10801 aaagatgtag gtcaaaatgt atgttatgaa tgcaaaggag agaaataatg aatgatttta
    10861 tcaaagagat tgggatggct atcctatgga tgtttttagg gtatctcttg ggagagcgta
    10921 gcactagagg gggacaaatc agatgatcaa taacgtcaca ctggttggga ggcttgtagc
    10981 gcctcctgat ctacgaaaaa cgcctaataa tgtatctagt ttacagggca cgcttgcagt
    11041 caatcgcaat ttcaaaaacg aaaatggaga gcgtgaggct gattttatca atttccaagc
    11101 gtggagaggt acagctgaca tcattgctca gtattgcagc aagggctcac ttattgggat
    11161 cattgggcgc ttacaagtta ggtcttacga gaaagacggt cagcgtcgat atgtgactga
    11221 agtaatcgct gagagtgtag ctctgctaga gagtcgcaac agtcagcacg gacaaggcaa
    11281 cagtttccaa aatgggaata gctcaccttt taccgatcct aacccctttg acctcccaaa
    11341 tgacggtttg ccgttttagg agggacagtt agtggaaata gataagatta taaaaaaaga
    11401 tgtcttggaa tttatggaaa caatttctga taataaaatt gatgcaagta aaatcaatgt
    11461 gacagaacgt attgctatca tcattgagaa acaaaaaata gaggtcgtta cgactctaaa
    11521 ctatgatatg agcattagct ttgataacaa agacacggct cctacactag atgacaatgg
    11581 tgaccttttt gaaccggtct acaagtgcaa agttaaggca attcccaaaa atgatgtatt
    11641 tttcacctca ttaacacggg tcaagagcaa taccaagacg ttacaagagg ttaaaaaatt
    11701 ctttgagttc gtaaacgaaa acagagaaaa tctctttgag atggcaggat ttaagggggt
    11761 tcttgaatga aattgaccct gaacattgag cctaaacctc aatcacggcc aaggtttgca
    11821 agacgtggga gttttaccac aacttacgaa gacaaggata tgaaaacatg gcgtaatcat
    11881 tgccagctgc tcattgctaa tcagtacatg ggtcagccta tccttgaggg agctttgagg
    11941 acacggctta gattttatat caaacctcct cagtacattt ctaaagtcaa gaagaaccaa
    12001 caggccctcc tggatgaaat tataccagta ggcaaaaagc ctgacataga taactacgaa
    12061 aaagcgctat atgacagcat gtcagggatc gtcttccagg acgacggtca gatagcgcta
    12121 catgatgtag gcaagttcta cagtctaaat ccacggatag aggttgagat tgaggtcatg
    12181 aaatccctga gtatttgaag aaatgaggag cagatggctg actacgcatt atatcaaggt
    12241 gatgtgtttg ttacgcttgg aacattagcg cagatcagta gcgagacagg aattactgaa
    12301 aggatgttaa agtattacac ttacacatca caccaaagac gacacccaaa cggtagggcc
    12361 gttattaaaa ttgaggagga agataatgag aattaagacg gaaagcggag gagttggaag
    12421 atgatggaag agttaaagca aaaagttaat gaagtataca actggacggt agaagacggg
    12481 aagccgcaac ctcccaagca agatttacca caagcggtga aagaccgggc ggactatttt
    12541 tgggaaatgg cagaagatgg tatgacgttt atgggagcga tggaatgcat cttcgctgat
    12601 gaaaagccta tagactatga tttgggagct actaaggatt ggttgccaaa atctaaggag
    12661 tttgatgatt gggttggtta ttcgccaagc atggctcagg tagttattgc agtttatttg
    12721 atttatagag gaaactaaga tgaataagca ggaattgatt aaaaaattag aggaacgaag
    12781 aacaataact gggaattttc aaggttatgt agtttggtgg aaggatgtaa aagaaatctt
    12841 tgaacaacta gacgaatccg aaataggtca cgcagatgaa gctccacgct acgtaaagaa
    12901 catactagca cgattgcgag aattgccatt gcatgataga gaggtttggt taaaggctat
    12961 catgagcgaa tttgaacagg attttagccg tgcaaaatgg cgagagggct acgagcaagg
    13021 taaaattgag ggtatggttg aacgtgaaaa agtcatagtt ccgcagtgtg tggcggaata
    13081 tatagaattt aaaaagaaaa acaattttca tgtttacggt gcaatgagag taattgaaga
    13141 tcattatgat aagaaagttc ctgattggtt ttacgaaaat aacatcgaaa aattctgtct
    13201 tgcttggctt gacggctacg aggttgaaaa agagaagcgg tattttgtta agattaaagg
    13261 gaatattaaa gaaaatatgt tggtttatgg agaacttttg aaaaggtatt tctttacaaa
    13321 aagctttagt ttagacgatg ttatatattc ccacacccgt aaagaactag aagacgcaaa
    13381 gttcggctgg gtgtttgatt gtccagggat tgagattgag gaggtggagt gatgagcctt
    13441 acgctaaata gcacaattgg agacttagtt ttggcaatcg gagaaattat cgttggttct
    13501 gatggtaaaa ccactacagc gatactggag atacctgatc aaagctttta cttagagatt
    13561 gagcttaaat tgaaggagga ggtcataaat tgaaacgatt catagctatc tggattctgc
    13621 tatctgctgg attgaatatc tggcagatgg acaggattcg agatttggaa gagaagaagc
    13681 cgatggttat ctacaaggct gataacgcag gcgttgagat attcggtaaa gtagtcgaga
    13741 aaggacggca tggcaagcta tatacgctaa cgatacgtga ctatggtgtg ttcgtggtta
    13801 cgaaggacgt gtacgataaa gtgaaagttg gggatgaggt gttactatga cagaaactat
    13861 taaactacca gactattatg agcctgattg gaaaaatgca aggtacgggt cgttggaaga
    13921 gcttaaagaa ttgttgctct ttaagcgtat tgtgaaatgg gataaggact ttttgctgct
    13981 tgaagacggc acaaaggtca ctattgaaat gtctgaaagt gattgctgtg cctcagcagg
    14041 tggggagttc caagatgtat cacttgacgc tgtgattact aatattgaaa ttggagaacc
    14101 ggaagaaatc cccgaccatt ggggaactgg ttataaaaac aaagtaacta tcttccataa
    14161 tcagaaccct gtagctattg ccaattgtga agcagagcat aacggctatt attacagcgt
    14221 atgctcttta gtgattggtg atattcattt tccagttgtt aatgcttagg aggatttaac
    14281 atgacaccaa gatatagagc gtggataaaa acagaaaaac gtatggtttt ttcagatgac
    14341 attcttgcta ttgactacga aaacaaagaa atagtgacac aacaagttta ttttgagaat
    14401 ggtttaccag acgatagaga tatctattgt tatgattttg acgaaatcga actcatgcaa
    14461 tcaacaggac tcaaagacaa gaacggcaag gaaatctttg aaggcgatat tgtacgaact
    14521 actagatttt tgggtagagc tgacgaaatt ggcggtttct atgagtatga caaggaattt
    14581 atagggattg ttaagcagct tgagggttct tgggtaattg atacgggcag tgacgcagta
    14641 tgtttatgga ctgaaattga agaaaatgaa atcatcggca acatctatga aaataaggag
    14701 tttggaggac gcaaatgaga ccttgtaaat atccatattc aggaagaaga aaaaaacaag
    14761 aaacgccgtc gccaatattt tctgcacgac caatttttga cgaagttcca attgtagaag
    14821 aagttaaggt tgagttcgga gttgaagcta gtatggggcg catatatcca gaaacgttaa
    14881 tacatttaga tatttctgga tacggaaata gagtgcattc agtacatcgc ttccccggta
    14941 ttttactgag tgttggtgag tcaatccaac taaagatgct tttctataaa agacttagaa
    15001 attttactac agatcgtttc ttgacgttta gagaatctga ttggaagttc tttatccggg
    15061 acctggtcaa cgaatttaag cattaaaaaa agccaagaca ctctctgcct cagctaatag
    15121 ttctcgcaaa gactattata tcacaaagga gacagagagt gaacaaggct aaagagctct
    15181 tgaaagagtt gcaggatctg gacatggaca tccaaagccg tatagacgaa attaacgagc
    15241 ttgaggcagg tttgctctca agccctaaat ggtccgaggt taaagtccaa ggtggacaga
    15301 ctagaaaagt tgatgatgtc tatactcagt tggtagtgat gaaagaggct atagagcagg
    15361 atactaaaga ggtcattaac agaaaacttg aattaggtag aatgatcaat aggcttaaaa
    15421 atccaaagta cagggcagta ttaagaatga cttacatcaa caaaggcacc gctgatagcg
    15481 tttgttatga tttgaatatg agtcgtacaa cctactacag gttaaaaaat gaggcggtct
    15541 tagctttgga agaagttatc taacctcata gtgagcttat gggacttttt ggaacagcac
    15601 ggttctaaaa atctgttaga atggtagtat caagaattga aaagagaggt ctcagaattg
    15661 gtagatggtt acctgtaatg tcagggggct gtaatggcct tggaggttca agtcctcccc
    15721 tctcctttga gtgtttgtgt cccagaatga gttaaatctt ctgggtgggg attcacatat
    15781 cactcattaa ctcctatcac tcattaactt aaaaatggtt gcggaagcga ctggacctcg
    15841 catgattgcg tagctaatta tattccggat aagttataag ctagagggtt tgattccctc
    15901 agaggtttta aagactacaa aaaataaaaa agaagtcaaa atttaatacg cacgcaaggt
    15961 agtagtcgcc ttgcaagaag gtcgcacatc gtgtggcttt ttttgattat tcgaagggtg
    16021 gtgatggaaa attgagtgga ttgagaataa aacaaaagag atttgcagat gagtacatca
    16081 tctcaggtaa tgcgacggaa gcctataaga aagcaggtta tcgtgtttct agtgatagag
    16141 tggcaggcgt tgaaggacat aagttactaa agaatcctaa gattaaaagc tatatagatg
    16201 aacgactgaa acagcttgat tctgaaaaga ttgcggatca gcaagaggtc ttaggttatc
    16261 taacttcagt catgcgagga gagacgcaag aacagacctt gataagcatt ggagaactag
    16321 ggcagacgat tacggatata gatgtaggag ctaaagatag aatcaaggcg gctgaacttc
    16381 ttggtaaacg gcataggctt tggacggata aggtagaggc ggatgtttct ggaacggtgg
    16441 tgtttgcaaa tgagtcagac atatcagatt aaacaaaacg atattgtcgt tgacttacct
    16501 aagacagtag gagctggata cggacagttc tggcgctcaa gaagtcttta tcgtgtagtc
    16561 aaagggtccc gtggttcgaa gaagtccaag acaaccgctt tgaattatgt tatccgtctt
    16621 ttgaagtatc cctgggccaa cttgcttgtt attcgtagat actcgaatac caacaagcaa
    16681 tcaacttata cggattttaa atgggcgtgt aatgtgttgg gtgtgactca tttgtttaaa
    16741 tttaacgagt ctttgcctga aataaccata aaagcgactg gtcaaaaaat cctattccgt
    16801 ggtttggatg atgaactcaa aattacatct attacagtcg atatcggtag tctgtgttgg
    16861 gcatggttcg aggaagcata tcaaattgag actgaagaca agttcagcac ggttgttgag
    16921 tctatccgtg gtagcttaga tgtacctgat ttctttaaac aaatcacagt cacatttaac
    16981 ccgtggaatg agaggcattg gctcaagcgt gtattcttcg atgaagagac gagacgggct
    17041 gacacattcg ctactacaac cacttataaa tgcaatgagt ggcttgatga agtcgatatc
    17101 aaacgctatg aggatttgta tcatacgaac cccagacgtg ctagaatcgt ttgtgatggc
    17161 gaatggggag ttgctgaagg tttaatctac gaaaacgtga ctgtcaagga tttcaataaa
    17221 gatgaattac tacaagattc agctaataaa ttatgtatcg gtcttgactt tggttttact
    17281 catgatccaa ctgctttgtg ttgttcgttg ataaatgaca cgacgaaaga gatttatgtc
    17341 tttgatgagg cgtataaagt cggattgata accaaagaag ttgcgaagat gataaaagac
    17401 aaaggttatc atcgctcaca aatcattgct gatagcgcag agtcacggct gattgaagag
    17461 ctcaggtcag aacatggcat atctagaata aaagagagtc ggaaaggtaa ggatagtatt
    17521 atggcaggcg tatccaaatt gcaaggatac gctatttatg tgcatccaga ttgtaaaaac
    17581 atcatggatg aattttatag ttactgctac cagcgagata aagaaggcaa ctggttgaat
    17641 aaaccagagg ataaaaacaa ccacttgatg gacgctttgc gttacagcct tcaatgtatc
    17701 gaaggtggaa aagcaaccgt ccgcagacgt tctgattatg gtctatagag aggaaagaca
    17761 tgtaccaata tttaacctat ccacgggatg gatatgatga gggttctttg aagaaagacc
    17821 tgatttacaa attgataacg atacataaca ctgaaagctc acgcttgaag aatttaaaaa
    17881 gctactacat gggtgatcat gctattttaa atcacaagag acgcaacgtg aacgcaccca
    17941 attacaagac ggtagctaat catgccaagg atatcgcaga cacggctacg ggctatttta
    18001 tgggcaatcc tatcaagtat aacaatactg ctgacggtga tattgatgaa ctacttacag
    18061 cctttgatgg tgctgagatt gaccaagtgg atgcgcagaa cgcactaaat atggctatct
    18121 acggccgtgc ttacgagtac atctatgcta aagagggtat gactgagttg gattcaacta
    18181 gtattgatcc ggagaatact ttcatggtct acgatgatag tattgagcgg aagcctttgt
    18241 ttgcggtcta ttactatgaa gtaaaagacg atacgaaaga cactaccaag caccaggctg
    18301 aggtctttac cgaaaatctg cactatcaca tggtgctgag aagtacagat tcaggaacaa
    18361 ctcagagcga ggaggcaaca cctcacaacc ttggtcaaat cccaattatc gagtatcgca
    18421 ataatcactt tgcgattggc gactacgagc aacaaattag cttgatagac gcttataatt
    18481 ccttgatggg gaatcgtgtc aatgataagg aacaggctgt agagtctatc cttgtcttgt
    18541 atggcacgca gttagcagac actccagaag acgctaaggt agcaatgaag attctttctg
    18601 aagaaggtct tttggaattg ccgggcgata gtgcaagggc tgagttcttg aagaatacgc
    18661 tggacgaaag tgctactgaa atcttgcgta cagctcttaa agaggacatc tacacattta
    18721 gccatgtgcc taatttgact gatgagaatt tcgcagggaa tacatcaggc gtagccatgg
    18781 aatttaagct gatgggcctt gagatgatta ctaagaccaa ggaagcgaac tataagcgag
    18841 gattgcgtca gcgtattgcg atttttgctc attacttagg tatgaagcag attgctttag
    18901 agtctcattc aatcgttcca caattcagtc gtggtttgcc taagaacttg ttagaaatct
    18961 ctcagattgt gaacaacttg gaaggcaaag tgaccaatag acagcttatt tctctcttgc
    19021 cgtttgtgga agaccctgac gctgagctgg aagccttgga agaagaaaaa aagaagaaca
    19081 tggaagacat gccgatgttc aaccaagaaa acacgaaacc cgaagacgag gtagaggatg
    19141 aagaatcagg agtattgggc gaagaggaaa gccaatctga tttaccagca gatggacaag
    19201 gccgaaaagc aggcagacca gttcgataag gtctatcagg aagccaagac ttacttggat
    19261 aaggaagtca ataagatttt tgataagttc caacgtgatt atggtctaag tcaggtagaa
    19321 gctagacaag tcttgaagaa catgaaagac aagaaaaatc tgaatgaact tcgtaaagta
    19381 cttgaagcga gaccgaatga tccaaatatc caaagactac tggctgactt agatagtccg
    19441 gcttattctt tccgtatgaa gcgtctagaa cgtttgagtg atgatttaga ccgtatgcgt
    19501 gaatctatct atcattcaga aaagacaggc tcagacgcct tttatagcga cttgatgaag
    19561 gatagttact acaaggctac ctttgacctg caacagcaga caggactagc atatggcttt
    19621 tctgggcttc ctgatagcga gattaaacat ctacagtctt tcagttgggt aggagatgga
    19681 agtacgtact caacaaacat ctggaagaat acagggaagc ttacatcaag cataaaagat
    19741 gaactactca tgagtctcat gactggtcga gatacacgag aaactgcaca agcaattgct
    19801 gagcggttca atgtaggcca gaatgatgca aggcgtttgg ttcggacgga atcagccttt
    19861 tttcataacc agatggaact actcagctat gaagaagcag acatagaaaa gtatatcttt
    19921 gtggccgtct tagacaagcg tacatcacgg atttgtcagg agcatgacaa tcaggtctat
    19981 gatagggaca aggctgtccc tggcgtcaat tgtccaccta tgcatccgtg gtgcaggtct
    20041 actactgtcg gatacgatga ggacgcagac tacagcaagt tgaagcgcag agcaaggaat
    20101 ccagagacag gtaaagttga gtacgtgcct gccgatatga cttataaaga gtggtatagc
    20161 aagtatgttg cgaaagacgg ggaaaaggtg tataatcaag atacaagaga agccaaggcg
    20221 aaattttata gcgaacaact attgtccaaa atttcaggag ttgagccaaa aattacaagt
    20281 gatatgcaac gtatcgcagg agaaaacaaa ttggcaggtc ttgaatttag gaagaaaaca
    20341 gttgagtcat tatcacgtaa aattattgca gatagcctag ttgaaaatat aagtttgtca
    20401 aaagccgtga gtaagattaa tgacgcctta aggtacacaa ctattttcga ttccgatact
    20461 tttacagaag agtatttgaa gatgaaacag aagcttatcg cagaaggtta taaaattgta
    20521 aaagtaaaaa acacttggct agtagatgga ccatacaaag gtgtgaatac agtcgttgaa
    20581 aaagatggta tcaactttga aatgcagtat catactcagg aaagtttcga cttaaaaaat
    20641 ggttcattac atgaactcta tgagaagtat cgtgatacga atacatctga tctagaacgc
    20701 atgaaattat ttaaggaaat gcttgattta agcaatgggc ttgagattcc taaaaatata
    20761 gagagggtga agtgatatga aagatattaa atactaccgc acaacgacga acaatgctca
    20821 agtacttcgt ttgattgatg gtgtcatgca agtttttgac attgaaaaaa agtgggttaa
    20881 tagcatggat tggtttaata aaatcttttt taatgacttt acggattttg aagaaatttc
    20941 agaaaatgat gcatttactt atattgacag gatggtagcg gcatgattga tattgccttg
    21001 gctatcgcta aaaaagcaca tgcagggcag gtagataaag cgggtgttga ttacatacag
    21061 catcctctct atgtggccag tcaagtcaac actgaacaag aaaaagctgt cgctctttta
    21121 catgatgtga ttgaggatag cgatataact gctgccgatt tattcgcgtc tggcttgtca
    21181 aatgaagttg ttacagcggt acaaattttg acaaagaaaa aaggtcaaag ttatcaagaa
    21241 tatcttggga aagtaaaatc aaataattta gcaagagttg taaaacttgc agatttgaaa
    21301 cataactcag atttatcacg tttgaaatct gttaccaata cagactacga gcgtgttaaa
    21361 aaatataaaa atgcaattta ttacttaagc acctagagaa atctaagtgc ttttttcgtg
    21421 ctcagaaagg agaatctgat gaataagtac aaaaagttga tagaattgat tgaaaataac
    21481 ggtcttgaga tacaatctaa gaaatgttat gatccacaga gtgcttggca tggtgaggag
    21541 ttatggattg ttgataagaa aaaacaaaat aaaatttttg atttatcagg taacggttac
    21601 tgttttcatg acgctaaagt tgaggaagcc attgaagaag ttgagaagta tctattattg
    21661 aaaaagatgg atacgtttga tgatttcaaa aaatgggtgg aaaagaatgc taagcctaaa
    21721 aaatgatgct tagaaaggag taaagacatg tttatatggg attgggtatc aatcgccttt
    21781 gggtggttgg tatttttgtt gttaatattt attattatgg ccgtaatcag cggaataatt
    21841 aaaggtgtaa agaaaggaac agaaaaatgg aagaatggaa agaaagattt aaaaaagaat
    21901 actacgaatt gaaagaacga ttccagaagt tagatatgat gattgggaaa tacgaaaaag
    21961 ggcaactaga gtttgaatct aaatgtccga ttgatttgtt aaaaggtcag cgttcaacca
    22021 tgtggaatta tttaagaatt ctagaacaac gtgcaaaaat tgaagaaatt aaactataaa
    22081 aattaaccgc atcgaaatcg aggcggtttt cttatgctct aaccgtatgg aatcccgtac
    22141 ggtttttata ttgtccaaac tgtgccgatg acattaaaag ctgtactgtt ccgtcgccgg
    22201 acgtaaagcg agattatcga gtggcgacgt aatcgctgga ggacaattat gtcagaagaa
    22261 atcaatgcaa ctgtatctac tgaatcaact gagactgtcg acactcaaga aaatgttgat
    22321 acagtgcagg aagaaaagca cgaacgaact ttcactcgtg ctgaaatcgg taagatgcta
    22381 tctgccgagc gctctaaatg ggaagctgag caagaagcca aggaaaacga agctaagaaa
    22441 ctcgccaaga tgaacgctga tgagaaacag aaatatcagt tagatcagcg tgagcaagaa
    22501 ctagccgacc gtgaaaaggc cattgctcgt aaggaattga ccgcagaggc taaagcaatg
    22561 ctaagtgaac gtgacttacc tgttgagtta gtaaatgtag tcgatttgac aaacgcagag
    22621 acggtatctg aatctatcac ctctatccaa aaagcatggg aagagtcagt tcagaaggga
    22681 gtctctgaac gtatgaaagg tagtgcacct atcaaaaatg cacaaacagt ccagcaagaa
    22741 gtcacggaaa aatggcgtaa agacttcttg taataaaaga aaagaggaaa aataaatggc
    22801 atttgaagaa ttaaacacag cagaatcacg caagaaacat cttgggatta ttgaggatgt
    22861 acttgcagta aattcatatt caacaccact tgtgacatca agcgatgcag taaccttgca
    22921 aggtcgctct tttacagtag caactggtaa cacaacagag ttgaaagact acaaacgtaa
    22981 caaagacaac gaatttgatc acgttgaagt tgaagaaaag gtttataccc ttgatgaaga
    23041 aaaatactgg ggtcgtttcg tagatcaatt ggacgaacgt gactctaatg gtcaagtgaa
    23101 tatcaattat gttattgccc gtcaggcggc agaagtagta gctccgtatc ttgatgaact
    23161 acgttttggt gcagcacttg gaaacgtaag tgacaatgtt gccatgggta aaacagcagg
    23221 agcgaacaac gcttataatg cggttcttga tgtgtctgag aaacttgatg agcttggaat
    23281 tacaaaagaa cgcttgctct tcgtcactcc aagtttctac aaagcgatca agtctgaaat
    23341 cgttcgtcta ccacatggtg acgcagataa gaaagtcctt ggaaaaggat atgttggtga
    23401 attggatgat tacacagtct ataaggttcc ttctaaattc ctgaaaggtg ttaatgccct
    23461 tgctactgct ccaggtgttg ttacatctcc agtacaagta gataatacta agtacaacga
    23521 taacattcca gggcgatttg gcgaattggt agagcaattg ctttacactg gtgcatttgt
    23581 tcttgaacac ttcaagaaat acatcatcac aattgcagat tctaagcctg ctgctaaaaa
    23641 atcagtacaa ggtaaagttg taaaccgtgc taaagcgtgg aaaactggaa caacttacaa
    23701 agaaggcgaa tcagtaactc atgccgataa agtctatgta gcggttaagg atatttctaa
    23761 ttcagcaacc gcaccagaca cagatacaac taactggaaa gaaaaaattg gtaagaaata
    23821 ggtccgagtt atgaaattta aaatcaaaca agatttctat gattgggaat caaatgtgaa
    23881 acgactggta ggggaggagc ttgagattac tgaggaacga tatgccgagc tggctgacaa
    23941 ttttgccagt aatggtgtcg ctatctcaga cgttcttgag gaaaccctcc ctgaacctga
    24001 gtttttagaa gaggattgat atgtctatag agttgctgaa gaaaatgaca ggcgaagaag
    24061 atactcagct tctcatgttg ctccaaacga gggctacaaa tcttatcttg tcagagacta
    24121 atcgcacatc tttgacacct gctttaagtc tcttaatacc tgaggttgct atcgagctcc
    24181 acaaccgctc aggagtggaa ggagagcatt ctagaaccga aggtggtata gcagtagtct
    24241 acggagaaaa cggcctgtct acgggccttt tacaacgtat ccgcatgcac agactagcaa
    24301 gggtggcagg ccatgttttt gaagcagagt agactgaaac cttatccaat gcgacggttt
    24361 gaaaagactg tcacagagga aggtgtcgca aaagaaggat atgtcaagga agctgagaca
    24421 gtccgtcttg aattgtggcc agctagtagc aagttgcaat ctgaattgta tggtgagcgt
    24481 gtcaatgata ttttgaatgc caatgccaac aaatcagcta ctatcaaagt gaaagatggt
    24541 gtgtgtatcg atagccagac agaagtgact cataaggtca tttctaaaaa ggtctacaca
    24601 catcatcaag ttttggagtt agagcgtgtc agagctacta ggggcagata ggcttatagc
    24661 taaatgtaga cgattggcta gtaaaaaaac tggcgaggat atcgtcttac gtgcggtaca
    24721 caatgctact ataaaggttg tccaagcaga tgcaagaaga ctcgcaccag cgagagatgg
    24781 agagcttata attagtatca aaactagagc aaaaatggac ggagataggg ctataggtga
    24841 agtttacact aatctaaaat atgctcctta cgttgaattc ggaacaggac caataggaca
    24901 agctaaccat tcgggtatct ctccagaggt cagcgtgact tacaagtcta atccttggta
    24961 tgtgcatgaa gaccaaatca atgtaggacc gtaccacttt caaaagattg gggagttcta
    25021 caagatgtat ggtcaacctg ctcagcctta tctttatcca gctttgagag acaatcaaga
    25081 gcgtgtgtct aagaatattt cgaattatgt ccgtagaaag ataagagaac aaataaaatg
    25141 attaatatca agcctgttat ttataaagaa ttgcaaaagg ttgcagataa tgtgactgat
    25201 acttatccta gcgattggga gactttccca gtcgttattt ttttagaaga acaaaacaag
    25261 ccgggtgatt ggtttgatga ccaggaacaa aaatcctcta tccgctacaa ggtggatatc
    25321 tttgatgata ccagcactag tgagttagct gttaaaatca ataagatttt tgagtcttta
    25381 ggtttacgca gaaccgactg ccaagacgtg ccagacccgt ctcatttgag acataaggtc
    25441 atgcgctttg aaggtgtcgt tgatttagac tcagagcttg tttttcaatt tagaatggag
    25501 aattaaacat gttagcaaat ggaattaaat tggcctttag tgaaacgaaa ggcaattatc
    25561 agaatcttgt agggcttaag gaagtacctg aatttggtat cgagcccgaa aaagtagaaa
    25621 atactaccct tgcagataca gtgaagaagt acgagtttgg tatcggggac gcaggagaac
    25681 ttgagtacaa gttcgcttat aataattcaa gcgcaacagc tccttaccgt gtattacgta
    25741 aggcagcaga tggtaagaaa aaactttact ttgagcaaac atatccagat ggtactaagg
    25801 tcacttttga aggtcaagta tctgttaagc ttggcggtgg cggtgtcaat gccgttatcg
    25861 agttcacact taagattgcc ttgcagtcta atcttacatt tactgatggt gttggaggtt
    25921 aattaaatgg cgttaaaata cacaacttgg aaagttactg acgaaaaaga gttgaagcta
    25981 cgtttgacat ctcatcaagc tgcaactgtg gaagaaaaaa tcggcatgaa cttgttaaag
    26041 attttcatgc ctgaagctgg cgaagagttc actttaccgc ctttgaaagt tatgttgttg
    26101 ttagttcacg gcgccttgca gcagtacgaa catgggtatt cctttgaaga tgtctacgac
    26161 ctatacgatg agtacgtcga taacggtgga gaccaaacaa cattcatgac agaggtgttg
    26221 atgccgctat ttgaagtatc gggttttact ccacgaggaa gcaaggacaa gaaaacttcc
    26281 aagaagaaaa tgacagtagt cgagtaatct taacagtaac gcagattatt gagaggcttt
    26341 atcctatgtt tttggacatc gggggcaagc ctcttgattt ttgggatttg acggtgcttg
    26401 aaatcagaga aatgattgaa agctataacc gtgtcaaaat ccaagagcgt aaagaaaaga
    26461 ttattgactc atacagactt tcgcagatga tatccaacca cgtttcttta ttgttatcca
    26521 aagatgccaa ggcctttgag ttctgggaat atgcgcctga gttgtttgta gaagaacaac
    26581 aagcagtaga acaggaacga cagagacaag cgcttttgtt gcataaggaa cggatgcgtg
    26641 attttgcaga gagacataat cgaaaaagga aggaggaagt aaatggcaac tcttgatgaa
    26701 ttgaaggtca tgattgacgc tgagatagcg cctttcagga aaaagatgaa agaagtcgag
    26761 aatcaggtca aaggaacatc tgaccaagtg aaaaatgcca ctgccaaagt tcgtgaacag
    26821 tcgaactcaa tcggtagtgc gtttggtaag ctggctaagt tcgctggttt tgcaatcctt
    26881 ggtaagaaat tacttgatgt tgggatgtat tcaacgcaga cggctcttga agtatcagcg
    26941 tctatgaacc aaatcaagcg acagatgggc gagagttcgc aatctttctt aaaatgggtt
    27001 aacgataacg ccaacgctat gaatatgggg gtgggtgagg ctactaacta cggtgcggtc
    27061 tactcaaact tattttctgg gtttatcaaa gataccaaca agctaagcgc ctataccgct
    27121 aagatgttgc agacatcggc agtggttgct gaaggttcag ggcgcacgat tacagacgtt
    27181 atggagcgga ttcgctcagg tttgctaggg aacaccgaag caattgagga cctaggaatc
    27241 aacgtcaatg tggctatgat tgagtctact gaagccttta agaagttcgc aaacggacag
    27301 agctggcaac agttggatta ccaaacccag caacaaatcc gccttatggc tattctggaa
    27361 caggctacag ccaagtatgg gaataccttg tctaattctg taaatggtcg tatcagccta
    27421 tttaagtcgc tgatgaagga cgcagcattg aaccttggta actctatgtt accgattatc
    27481 aatgccatta tgcctgtctt gaactctttt gctatggttt tgaagaacgt gacggctaaa
    27541 ctcgctgagt ttatcgcttt gatgttcaac aagaaagcaa cagtgaaaga tggtgttggt
    27601 ggagcagttg gagacatggg taacgccatg aaagacgctg caggcggagc aggagacctt
    27661 gctgacgcag tagacgacgc tggagattca gcaggaggac ttgctgacaa tcttggagac
    27721 tccgccaaaa acgctaagaa ggccgctaaa gagttgctag gtcttttggg atttgatgag
    27781 attaacatct tgcaaaaacc aaaagatgac gatgcaggcg gttctggagg cggtggcgga
    27841 cctttcaaag acatcttgcc agaagtcgag ttgaccgaca tggacaacaa attcaagagc
    27901 atttttgatg gtcttggaga taagctcaaa gggttgtttg acctcttcaa gaaaggtttt
    27961 gatgcagcat ttagaccaga aggtataaaa cgcattaaga ctgccttaga ccaaatagct
    28021 aagacaatgg gagaaatcgc cactgaccca agggttgtga atgcctttaa ccgcatggct
    28081 gagaaaattg cttatgcttt agggcaagtg acaggctcaa taaccactat cgggctaggt
    28141 atcggtgttt tccttgccga aagtattgca aatggccttg gaaggcaaaa agaacgcatt
    28201 atcagggcgc tagtcgcttt gtttgataat gttggtaacc tttccgaggc agtaggaaac
    28261 atagctcagg acttttctag tgctttctac gacgtcatta cctcaactgg tgcggttcgt
    28321 atcggtagcg ctattgtgtc aactctgttg agtttgacat ctaccattgt tgaagttggt
    28381 agtaaattag caggaagttt gtttaaaggt tttgaaaaag tcgttgtgac aagcgctcct
    28441 aaaatttcat cagtcttcca aagtttatta gatactgttg cgcctgtatt tgagaacatt
    28501 gaaaggtctg ttaacaaatt tggcgatggc ttaagtcgtg tttatgatga acatgtagtc
    28561 cctgctatta actctattgc taatgctttt aatgggctaa ttgacattat tcagattctc
    28621 tgggagaatt cctggcaacc ttttgctgag tttttatcag gagtattcgg tgttagtatt
    28681 gaaggaattt cagatttatt aggaggtggc cttttagcca ctttgggact attggcggat
    28741 gctattaagt tagtggcaga tggtttcacc gttttttctg actggtgtaa agaaaacaaa
    28801 gaacctatcg tagctttgat aacaacttgg caaacgatta atttcttatc atgggcagaa
    28861 caagctggag gacttgcagg agcattcagc ttgttaggta gtaaggtctc tttgattgtt
    28921 ggagggatta agaatctagg tcttgctatt aaagcattga catttgataa gttggtcagt
    28981 tttggtgaaa caatctattt gaacacctta tatgcaaaag attttgtggt caattcaggt
    29041 aaaacaattg cacagctagg aaaaactgct ttagaacttg gtaaatcagc tctagcatgg
    29101 actgctcatg cagcgaaaat gggattagca accgcggcgg aatttgcaca ttctgttgca
    29161 gcaggagtcg ctacagctgc aacatgggct tttaatgcag cgttagcagt tttgacaagt
    29221 ccaataacat ggattattgc agcaatcgca gctttgattg ctattggtgt tttgctctat
    29281 caaaactggg acactgttgt tgagtttgct aaaactgcat ggcaaggact atgtgatttt
    29341 atcagtggta tttgtcaagc gattggcgaa tttttcagcg gtctatggac gaaactacaa
    29401 gaaatctttg agccgatagg tcaatggttt ggcgagaaat tccagcaagc atgggacgcc
    29461 attgtaaaca tattctctgg catcggagag tggttctctg gtgtattcca aggtgcatgg
    29521 gacgctatcg ttaatatctt cacaccaatc ggctcatggt tcggacaacg ttgggcagat
    29581 gtgactaatg cgttatccag tgtttcaaac tggtttggtg agatgttcac taatgcttac
    29641 aacgcagtaa aagatgcttt tagttctatt ggcgacttct ttaaaggcgt ttgggatact
    29701 gttaaaagta tcttcgtaaa tgctggtcag atggtcggag aggcagtagg tggagcgttt
    29761 aagagtgcgg tcaatgcggt tcttggaacg attgaaaatg tagtcaatgg cttcatcgga
    29821 atgattaatg gagttttagg cgttgtcaga aacttacctg gtctaggatg ggttggtagt
    29881 gtaagcacag ttagcctccc tcgtcttgcc cgtggtggta tcgtcgatag tccaacaatc
    29941 gccatgattg gtgaagctgg taaagaggcg gtcgtaccac ttgaaaatac aggatttatc
    30001 caaacacttg gacgagtagt cagcagtgcg gtagtaaatg ccatggctgg tgttagtcca
    30061 caaggtggat tctctggcga cggcgacatc gttatccaaa tcgcaggcca tgagttcgga
    30121 cgggtagcca tccaagaaat caacaaggaa catgaacgag caggtcaaac cttgctcaag
    30181 atttaggagg ttaaatggca caattgacaa tcaatggggt ggctgtgaag cctcccaaat
    30241 cttttcaagt cggtattcaa gatatcgatg gagagacagg gcgtaatgcc aatggcgaca
    30301 tggtgcgtga ccgtatcacg accaaacgca aactagactg tgaatggggt atgatgactc
    30361 agggagaaat aagtcagctt ttacatgctg tatcatctaa attttttgag gtatcttatc
    30421 cagaccccat ggatggccaa gtcacaaaga ctttctatgt cggtgatagg acagctccta
    30481 gctatacctt tactgagaag tttaaacctt ggtctggcgc taaatttaat ctggtagaga
    30541 ggtaagaaaa tggacgcttt aactagacga caatttgaca gagccatgtt tgccaaggaa
    30601 aggacgctgg ctattcgtgt tggtgattat acttcacggg atatcaaaga ggctagtttt
    30661 gagtatggct acatcaaggg cgatacttat aagcctggtg gaacctgcgc tggtagcggt
    30721 aaaattacct ttaccagtat cattaccacg ttcaataagc tggataccct gcaccctgag
    30781 attggtctac tggttgggga tacctaccag tgggtcaaga tgggggaata cttcatcaac
    30841 gatattgaga ttgaccgaaa ccgcaacaca accacacttg aacttatgga cggtatgttt
    30901 aagctcaatc gtgagtacgt gacggatttg catttcccag ctgaagtacg agaggttatt
    30961 caggaaatct gcctgaaaac aggcattgag ttagcgaatg actatttcgg aatcagcgcg
    31021 atgcgttatc atattgagca agttcctgag ggcaagaaac tttcctttag ggatatgctg
    31081 agcgctatga ctcaggtgat tgggatgtct tgtttcttca acagagaagg caagatggaa
    31141 atccgtgatt tgactgagtc caatatcacg atcaacgctg acagttattt cttgcatggc
    31201 ttgaccaaga gtgagattga gtatcagata gctggtatca cttgtaagac ggacaagaag
    31261 tctctgacgg tcggtatgaa gacaggccgg tctttggaac tggacaatgt cttcatgacc
    31321 cagagcgctt taaatgacct gtattacaaa ctgaaaaacc taacttacta tccgtataat
    31381 ctcaactacc aagggcattt actgcttgag gtcgggcagt gggtaaccat tcagaccaac
    31441 aagaaagaga cttttaaagt tcctgtctta agtcagagct ttatctttaa aggtggtctg
    31501 agaggtcgta tcagtgcaga tagtaaggct ggaaacgata cccagtattc ttacgagggt
    31561 acgattacca agcagattaa gcaacaagat ggctttgaag cgaaaatcca agcgcagatt
    31621 gaagcagcag ataaagattt tgaccaaaag gtcgacaaaa tcaaaaaaga ctttaacgat
    31681 caagtagaac tgaccaaagc cagagctgaa gaagtcaaga gagaactgtc tgacactatc
    31741 aatcagcgct ttaatagctt tgacaacggg ccattgaaag aagctaagcg caaggctgag
    31801 gaagctttgc gaaatgctgg cgcaagtacc ctgcttgcac aggaagctaa gcggattg
//
LOCUS       DAVLYV010000015        31569 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLYV010000015 DAVLYV010000000
VERSION     DAVLYV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.015"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..221
                     /locus_tag="VYO70_000730"
     CDS             <1..221
                     /locus_tag="VYO70_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1712998.1"
                     /translation="SQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTF
                     LSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(418..1338)
                     /gene="cysK"
                     /locus_tag="VYO70_000731"
     CDS             complement(418..1338)
                     /gene="cysK"
                     /locus_tag="VYO70_000731"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1712999.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1623..2258)
                     /locus_tag="VYO70_000732"
     CDS             complement(1623..2258)
                     /locus_tag="VYO70_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1713000.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQIDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            2314..3612
                     /locus_tag="VYO70_000733"
     CDS             2314..3612
                     /locus_tag="VYO70_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1713001.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     SIILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKATKEIQMMNKEAGL"
     gene            3609..4271
                     /locus_tag="VYO70_000734"
     CDS             3609..4271
                     /locus_tag="VYO70_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1713002.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4351..4899
                     /gene="raiA"
                     /locus_tag="VYO70_000735"
     CDS             4351..4899
                     /gene="raiA"
                     /locus_tag="VYO70_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1713003.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNVVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            5035..7008
                     /locus_tag="VYO70_000736"
     CDS             5035..7008
                     /locus_tag="VYO70_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1713004.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            7005..7457
                     /gene="rplI"
                     /locus_tag="VYO70_000737"
     CDS             7005..7457
                     /gene="rplI"
                     /locus_tag="VYO70_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1713005.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7501..8853
                     /gene="dnaB"
                     /locus_tag="VYO70_000738"
     CDS             7501..8853
                     /gene="dnaB"
                     /locus_tag="VYO70_000738"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1713006.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            8855..9121
                     /locus_tag="VYO70_000739"
     CDS             8855..9121
                     /locus_tag="VYO70_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713007.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9209..10555
                     /gene="cbpD"
                     /locus_tag="VYO70_000740"
     CDS             9209..10555
                     /gene="cbpD"
                     /locus_tag="VYO70_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1713008.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10638..10740
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10811..11101
                     /locus_tag="VYO70_000741"
     CDS             10811..11101
                     /locus_tag="VYO70_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1713009.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            11064..11822
                     /locus_tag="VYO70_000742"
     CDS             11064..11822
                     /locus_tag="VYO70_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713010.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            11860..12867
                     /locus_tag="VYO70_000743"
     CDS             11860..12867
                     /locus_tag="VYO70_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1713011.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            12867..13595
                     /locus_tag="VYO70_000744"
     CDS             12867..13595
                     /locus_tag="VYO70_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713012.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13714..14172
                     /locus_tag="VYO70_000745"
     CDS             13714..14172
                     /locus_tag="VYO70_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1713013.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            14174..16606
                     /locus_tag="VYO70_000746"
     CDS             14174..16606
                     /locus_tag="VYO70_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1713014.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLISKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLTE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17248..17901
                     /gene="rr06"
                     /locus_tag="VYO70_000747"
     CDS             17248..17901
                     /gene="rr06"
                     /locus_tag="VYO70_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1713015.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            17898..19238
                     /gene="hk06"
                     /locus_tag="VYO70_000748"
     CDS             17898..19238
                     /gene="hk06"
                     /locus_tag="VYO70_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1713016.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19321..19848
                     /locus_tag="VYO70_000749"
     CDS             19321..19848
                     /locus_tag="VYO70_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1713017.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            19927..22083
                     /gene="cbpA"
                     /locus_tag="VYO70_000750"
     CDS             19927..22083
                     /gene="cbpA"
                     /locus_tag="VYO70_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpA"
                     /protein_id="HEV1713018.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGST
                     QVATSSNMAKTEHRKAAEQFDEYINKMIQLDRRKDTQNRTLIIKLSAIKTEYLRELNV
                     LEYKSKAELPSKIKAKLDAAFDQFKKDTLPTEPGKKVAEAKKKVEEAEKKVAEAKKKA
                     KAQKEEDYRNYPTITYKTLELEIAEFDVKVKEAELELVKKEADESRNEGTINQAKAKV
                     ESEKAEATRLKKIKTDREKAEEEEAKRRADAKLKEAVEKNVATSEQDKPKGRRKRGVP
                     GEQATPDKKENDAKSSDSSVGEEALPSPSLKPEKKVAEAEKKVAEAEKKAKAQKEEDH
                     RNYPTNTYKTLELEIAESDVKVKEAELELVKEEAKESRNEEKVNQAKAKVESKKAEAT
                     RLEKIKTDRKKAEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAPKPE
                     NPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGM
                     WYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQYN
                     GSWYYLNANGSMATGWLQYNGSWYYLNANGDMATGWLQNNGSWYYLNANGDMATGWLQ
                     YNGSWYYLNANGSMVTGWLQYNGSWYYLNANGSMATDWVKDGDTWYYLEASGAMKASQ
                     WFKVSDKWYYVNGSGALAVNTTVDSYRVNANGEWVN"
     gene            complement(22313..23293)
                     /gene="dusB"
                     /locus_tag="VYO70_000751"
     CDS             complement(22313..23293)
                     /gene="dusB"
                     /locus_tag="VYO70_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1713019.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLID
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(23280..24152)
                     /gene="hslO"
                     /locus_tag="VYO70_000752"
     CDS             complement(23280..24152)
                     /gene="hslO"
                     /locus_tag="VYO70_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1713020.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKIAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGVEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            24317..25744
                     /locus_tag="VYO70_000753"
     CDS             24317..25744
                     /locus_tag="VYO70_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1713021.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            25903..27411
                     /gene="glpK"
                     /locus_tag="VYO70_000754"
     CDS             25903..27411
                     /gene="glpK"
                     /locus_tag="VYO70_000754"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1713022.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMIKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            27453..29279
                     /gene="glpO"
                     /locus_tag="VYO70_000755"
     CDS             27453..29279
                     /gene="glpO"
                     /locus_tag="VYO70_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1713023.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            29349..30053
                     /locus_tag="VYO70_000756"
     CDS             29349..30053
                     /locus_tag="VYO70_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1713024.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            30350..30553
                     /locus_tag="VYO70_000757"
     CDS             30350..30553
                     /locus_tag="VYO70_000757"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713025.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEGKKQELLNRLNK"
     gene            complement(30640..31227)
                     /locus_tag="VYO70_000758"
     CDS             complement(30640..31227)
                     /locus_tag="VYO70_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713026.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         9708 a         5560 c         7176 g         9125 t
ORIGIN      
        1 cgagtcaata ctatttggag cccctttata acctcttgcg agagagacta ttgactcagc
       61 ccttacttca tgcggatgaa acttcttata gggtgctaga gagtgatagt cagctgactt
      121 actattggac ttttttgtct gggaaagctg agaaacaagg gattacgctt taccatcatg
      181 tcctaatcga tttgttcata tcttatttca atccattata aatagtgaga aatatctatc
      241 ctatcttcta gaatgtcttc caaacgtgga aactctcgta aacaaagagg ttttagaggc
      301 ctatttacca tggattaaag ttgtacaaga aaagtgcaaa taagaaatct ccagattagg
      361 aactatccgt gagttctcta gtctggagat ttttcaatat acttcgttat tggacggtta
      421 caattcataa agtgctgtag agagataacg ttcaccgtta tctggtgcta gggcaaggac
      481 ttttttacct gtacctaatt ttttggcaac ctcgatggct ccgtagatag ctgcagctga
      541 ggaaatccct acaaggaagc cttcttttcc accaatttca cgtccgagtg caagagcgtc
      601 atctgatgtt acacgaacga taccatcata ggctttagta tcaagtgtat caggaataaa
      661 tccagctgag ataccttgaa ttttgtgagg accgggtttt tcaccagata gaatagcaga
      721 ttcatctgct tctactgcaa aaacttgaat gttagaattt tctgatttga gtgcatgaga
      781 aacaccagaa atcgttccac cggtacctac tccagcaaca aaggcatcta atccatcttt
      841 accgaaagca gctagtatct cagctcctgt tgttctttcg tgtacttctg gattagctgg
      901 attgtcaaat tgaagaggaa ggaaaccatc acgttcagca gcgatttctt gagccttagc
      961 aatagcacct ttcattccct cgctaccagg agttaggacg agttcagcac cataagcttg
     1021 gataatttta cgtcgttcta cactcatagt ttcaggcata acgatgacga ctttataccc
     1081 tttagcagca cctacccatg aaagtccaat accagtgttt ccacttgttg cttcaacaat
     1141 agtagaacca ggtttcagaa taccatcttg ttcagctttt tcaatcatgc taagggcaat
     1201 acggtctttt acagatgaac caggattaaa tgcttcaagc tttatataga cgtctgcagc
     1261 accttctggc acgatgttgt taagtttaac aattggtgtt tgaccgatta attcagtaat
     1321 gttgttataa atagacatat gaatacctct ttgtataaga tatctttagt ataacgctat
     1381 ctataccatt tgtaaaatat agaaatccta tcgaagcgat aggatttcta tatatcatag
     1441 atcaaagtca ttaattttca agtgattgcg ttcagctaat tctagtaaat aggtgtgaag
     1501 gaaaacgata tcaggtttcg tttgaattgc ttcattttct agtatatttt ggtacgcatc
     1561 tttttatttt gctcgctaaa gtagttgtga gaatgtcaac gaaacgttgt attattcatt
     1621 gtttacacta agtttacagg aacttcaacc tctcgtaaac cttggtcagt taaagtgact
     1681 tttccattaa aaaactccac aagtgcagct ttaatagttt ctttttcttc tttatcaaca
     1741 taaatcatcg tatcgatttg atctgtaaag tttgtatcca gctccatgag atcatgttct
     1801 ttaaggaagt tactgtactc ttggtactga gcataagaca tttgaatagc aatgccagcc
     1861 tgttctttta tttcaataat accaatttct ttgacagcta aggcgacact gccggcgtaa
     1921 gcacgaatta gtcctccagc gcctagttta ataccaccaa agtagcgtgt cacgaccaca
     1981 cagacattgg tgagattgtg attttctagt accccaagca tgggaacacc agcagtacca
     2041 ctaggttcac catcatcact tgtacgttta atttcactac gttctccaat aataaaggca
     2101 gagcagttat gtgtcgcttt gtagtgttct tttttgatgg cagtaatgaa gtcacgagcc
     2161 tcttcttcgc tataaacacg cttggcatgg cagataaagc gagatttttt gatttcttct
     2221 tggacttgac cgtcctcttt aattgttcta aattccatga tttaattgta ctaaaaaatc
     2281 atctaaagtg ctagttttta cgaataaaga agtatgaaag taaatttaga ttatctcggt
     2341 cgtttattta ctgagaatga attaacagaa gaagaacgtc agttggcgga gaaacttcca
     2401 gcaatgagaa aggagaaggg gaaacttttc tgtcaacgct gtaatagtat tattctagaa
     2461 gaatggtatt tgcccatcgg tgcttactat tgtcgagagt gcttgctgat gaagcgagtc
     2521 agaagtgatc aaactttata ctattttccg caggaggatt ttccgaagca agatgttctc
     2581 aaatggcgcg gccaattaac tccttttcaa gagaaggtgt cagagggact gcttcaagca
     2641 gtagacaagc aaaagccaac cttagttcat gcggtaacag gagctggaaa gacagaaatg
     2701 atttatcaag tagtggctaa agtgatcaat gcgggtggtg cagtgtgttt ggctagtcct
     2761 cgcatagatg tttgtttgga gctgtacaag cgcctgcaac aggatttttc ttgcggaata
     2821 gctttgctac atggagaatc ggaaccttat tttcgaacac cactagttgt tgcaacaacc
     2881 catcagttat tgaagtttta tcaagctttt gatttgctga tagtggatga agtagatgct
     2941 tttccttatg ttgataatcc catgctttac cacgctgtca agaatagtgt aaaggagaat
     3001 ggattgagaa tctttttaac agcgacttcg accaatgagt tagataaaaa ggtccgttta
     3061 ggagaactaa aaagactgaa tttaccgaga cggtttcatg gaaatccgtt gattattcca
     3121 aaaccaattt ggttatcgga ttttaatcgc tacttagaca agaatcgttt gtcaccaaag
     3181 ttaaagtcct atattgagaa gcagagaaag acagcttatc cgttactcat ttttgcttca
     3241 gaaattaaga aaggggagca gttagcagaa atcttacagg agcaatttcc aaatgagaaa
     3301 attggctttg tatcttctgt aacagaggat cgattagagc aagtacaagc ttttcgagat
     3361 ggagaactga caatacttat cagtacgaca atcttggagc gtggagttac cttcccttgt
     3421 gtggatgttt tcgtagtaga ggccaatcat cgtttgttta ccaagtctag tttgattcag
     3481 attggtggac gagttggacg aagcatggat agaccgacag gagatttgct tttcttccat
     3541 gatgggttaa atgcttcaat caagaaggcg actaaggaaa ttcagatgat gaataaggag
     3601 gctggtctat gaagtgcttg ttatgtgggc agactatgaa gactgtttta acttttagta
     3661 gtctcttact tctgaggaat gatgactctt gtctttgttc agactgtgat tctacttttg
     3721 aaagaattgg ggaagagaac tgtccaaatt gtatgaaaac agagttgtca acaaagtgtc
     3781 aagattgtca actttggtgt aaagagggag ttgaagtcag tcatagagcg atttttactt
     3841 acaatcaagc tatgaaggat tttttcagtc ggtataagtt tgatggagac ttcctgttaa
     3901 gaaaagtttt cgcttcattt ttaagtgagg agttgaaaaa gtacaaagag tatcaatttg
     3961 ttgtaattcc cctaagtcct gatagatatg ctaatagagg atttaatcag gttgagggct
     4021 tggtagaggc agcaggcttt gagtatctgg atttattaga gaaaagagaa gagagagcca
     4081 gttcttctaa aaatcgttca gagcgcttgg ggacagaact tcctttcttt attaaaagtg
     4141 gagtcactat tcctaaaaaa atcctactta tagatgatat ctatactaca ggagcaacta
     4201 taaatcgtgt taagaaactg ttggaagaag ctggtgctaa ggatgtaaaa acattttccc
     4261 ttgtaagatg aggaaaaaat ttgcaaaaat agaatgaaag cgttataata aaatcataaa
     4321 aacaaaaatg tttagaaaga aggtactcat atgattaaat atagtatccg tggtgaaaac
     4381 ctagaagtaa cagaagcaat tcgtgattat gtagtttcta aactcgaaaa gatcgaaaag
     4441 tacttccaac cagaacaaga gttggatgcc cgaattaact taaaagttta tcgtgaaaaa
     4501 acggctaaag tggaagtaac gattccgctt ggatctatta ctctccgcgc agaagatgta
     4561 tctcaagata tgtatggttc aattgacctt gtaactgata aaattgaacg tcagattcgt
     4621 aaaaataaaa caaaaatcga gcgtaaaaat aaaaataagg tagcaactgg tcaattattt
     4681 acagatgctt tggtggaaga ttcaaatgtt gtccagtcta aagttgttcg ttcaaaacaa
     4741 attgatttaa aaccaatgga tttggaagaa gcaattctac aaatggattt attggggcat
     4801 gatttcttta tctatgtgga tgttgaagat cagacaacca atgtgattta tcgtcgtgag
     4861 gatggcgaaa ttggtttgtt agaggttaaa gaatcttaat ttcaatatgt gtaaaggtag
     4921 gtttactgaa ttgtaaacta cctttttctt ataattgata gaattactga ttacactttt
     4981 aaaaagtcgc tttttgaggg gtattatggt ataatgggtg ccaagaggtt ttgaatgaaa
     5041 aaattttatg taagtccaat ttttcctatt ctagtaggat tgattgcgtt tggagtctta
     5101 tccactttca ttatttttgt taataataat ctgttgacgg ttttaatttt gtttcttttt
     5161 gtaggaggct atgttttttt atttaagaaa ctgagagtgc attatacaag gagtgatgta
     5221 gaacagatac agtatgtaaa ccaccaagcg gaagaaagtt tgacagctct attggaacag
     5281 atgcctgtag gtgttatgaa attgaattta tcttctggag aggttgagtg gtttaatccc
     5341 tatgctgaat tgattttgac caaggaagat ggtgattttg atttagaagc tgttcaaacg
     5401 attatcaagg cttcagtagg aaatccgtct acttatgcca agcttggtga gaagcgttat
     5461 gctgttcata tggatgcttc ttccggtgtt ttgtattttg tagatgtatc cagggaacaa
     5521 gccataacag atgaattggt aacaagtaga ccagtgattg ggattgtctc tgtggataat
     5581 tatgatgatt tggaggatga aacttctgag tcagatatta gtcaaatcaa tagttttgta
     5641 gctaatttta tatcagagtt ttcagaaaaa cacatgatgt tttctcgtcg ggtaagtatg
     5701 gatcgatttt atctatttac tgactacacg gtgcttgagg gcttgatgaa tgataaattt
     5761 tctgttattg atgctttcag agaagagtcg aaacagagac agttgccctt gaccttaagt
     5821 atgggatttt cttatggcga tggaaatcat gatgagatag ggaaagttgc tttgctcaat
     5881 ttgaacttgg ctgaagtacg tggtggcgac caggtggttg ttaaggaaaa cgacgaaacg
     5941 aaaaatccag tttattttgg tggtgggtct gctgcttcaa tcaagcgtac acggactcgt
     6001 acgcgcgcta tgatgacagc tatttcagat aagattcgga gtgtagatca ggtttttgta
     6061 gtcggtcaca aaaatttaga catggatgct ttgggctctg ctgtaggtat gcagttgttc
     6121 gccagcaatg tgattgaaaa tagctatgct ctttatgatg aagaacaaat gtctccagat
     6181 attgaacgag ctgtttcatt catagaaaaa gaaggagtta cgaagttgtt gtctgttaag
     6241 gatgcaatgg ggatggtgac caatcgttct ttgttgattc ttgtagacca ttcaaagaca
     6301 gccttaacat tatcaaaaga attttatgat ttatttaccc aaaccattgt tattgaccac
     6361 catagaaggg atcaggattt tccagataat gcggttatta cttatatcga aagtggtgca
     6421 agtagtgcca gtgagttggt aacggaattg attcagttcc agaattctaa gaaaaatcgt
     6481 ttgagtcgta tgcaagcaag tgttttgatg gctggtatga tgttggatac taaaaatttc
     6541 acctcgcgag taactagtcg gacatttgat gttgctagct atctcagaac gcgcggaagt
     6601 gatagtattg ctatccagga aatcgctgcg acagattttg aagaatatcg tgaggtcaat
     6661 gaacttattt tacaggggcg taaattaggt tcagatgtac taatagcaga ggctaaggac
     6721 acgaaatgct atgatacagt tgttattagt aaggcagcag atgccatgtt agccatgtca
     6781 ggtattgaag cgagttttgt tcttgcgaag aatacacaag gatttatctc tatctcagct
     6841 cgaagtcgta gtaaactgaa tgtacaacgg attatggaag agttaggcgg tggaggccac
     6901 tttaatttgg cagcagctca aattaaagat gtaaccttgt cagaagcagg tgaaaaactg
     6961 acagaaattg tattaaatga aataaaggaa aaggagaaag aagaatgaaa gtaatctttt
     7021 tagcagatgt taaaggaaaa ggtaaaaaag gcgaaattaa ggaagtacca acagggtatg
     7081 cgcaaaactt tcttatcaaa aagaatctag ccaaagaagc gactgctcaa gctgtaggtg
     7141 aacttcgagg taaacaaaaa tcggaagaaa aagctcacgc tgagatgatt gcagaaggaa
     7201 aagcaattaa agcacaactt gaagcagaag aaactgttgt agaatttgtt gaaaaagttg
     7261 gtccagatgg tcgtaccttt ggttctatta ccaataagaa gattgcagaa gaattgcaaa
     7321 agcaatttgg aattaagatt gataaacgtc atattcaagt acaagctccg attcgagcgg
     7381 ttggtttgat tgatgtgcca gtgaaaatct atcaagatat cacaagtgta atcaatcttc
     7441 gtgtgaaaga aggataagtt tacactttct tgacaagatt gtaaaaggaa gggaagtctg
     7501 atggcagaag tagaagagtt acgagtacaa cctcaagata tcttagctga gcaatccgtt
     7561 ttaggggcta tctttattga tgagagtaaa cttgtttttg tgcgagaata cattgagtct
     7621 cgggactttt ttaagtatgc ccatcgtttg attttccaag ccatggtcga tttatccgat
     7681 cgtggtgatg ccatagatgc aacaacggtt cgtactatcc ttgataatca aggtgattta
     7741 cagaatattg gtggcttgtc ttacttggtt gagattgtta attctgtgcc aacttctgct
     7801 aatgcggagt attatgctaa gattgttgca gaaaaagcaa tgctacgtcg tttaattgcc
     7861 aagttgacag agtctgtcaa ccaagcttac gaagcgtcac aaccagctga tgaaattatt
     7921 gctcaggcag aaaaagggct gattgatgtc agtgaaaatg caaatcgaag cgggtttaag
     7981 aacattcgag atgtgttgaa tctcaacttt ggaaatctgg aagctcgctc gcaacaaacg
     8041 accgatatta caggtattgc gacaggttat cgtgatttgg atcatatgac aacaggactt
     8101 catgaggagg agttgattat cttagcagct cgtccagcag ttggtaagac agcatttgcc
     8161 ttgaatatcg ctcagaacat tgggactaag ttggacaaaa cggttgctat tttttcactc
     8221 gaaatgggtg cggaaagctt ggtagatcgt atgttagctg cagaaggctt agtggagtca
     8281 cattctatcc gtacagggca attgacagat gaggagtggc aaaaatatac tattgctcag
     8341 ggtaatctag ctaacgccag tatctatatc gatgatacgc caggtattcg gattacagag
     8401 attcgttctc gttctcgtaa attggctcaa gaaactggaa atcttggttt gattttgata
     8461 gactatttgc aacttatcac gggaactggt cgagaaaatc gtcaacaaga agtttctgaa
     8521 atttctcgtc agttgaaaat actagccaag gaattgaagg ttccagtaat cgctctgagt
     8581 cagctttctc gtggtgtaga acaacgtcag gacaagagac cggtcttgtc tgatattcgt
     8641 gaatctgggt ctattgagca ggacgctgat atcgtagctt ttctctatcg cgatgactac
     8701 tatgaacgtg gtggtgaaga agaggagggt atcccaaata ataaggtgga agttattatc
     8761 gagaaaaacc gtagtggagc tcgtggaaca gtggaattga ttttccaaaa agaatacaat
     8821 aaattttcaa gtatctcaaa gagggaggca taagatgtca gatgcattta cagatgtagc
     8881 caagatgaaa aaaatcaaag aagaaatcaa ggcacatgag ggacaagtcg tagaaatgac
     8941 tttggagaat ggtcgtaagc gccaaaaaaa tagattgggt aagctaattg aagtttatcc
     9001 atctctattt attgtggagt ttggggatgt ggaaggagat aaacaagtta atgtttacgt
     9061 tgaatccttt acttactcag atattcttac agaaaagaat ttgattcatt atcttgacta
     9121 aagtgagaaa ttttctcact ttttcttttt ctccgaataa tttaggtgaa ggcaatcatc
     9181 gctttatatt atttttcaag gaggaagaat gaaaatttta ccgtttatag caagaggaac
     9241 aagttattac ttgaagatgt cagttaaaaa gcttgttcct tttttagtag taggattgat
     9301 gctagcagct ggtgatagtg tctatgccta ttccagagga aatggatcga ttgcgcgtgg
     9361 ggatgattat cctgcttatt ataaaaatgg gagccaggag attgatcagt ggcgcatgta
     9421 ttctcgtcag tgtacttctt ttgtagcctt tcgtttgagt aatgtcaatg gttttgaaat
     9481 tccggcagct tatggaaatg cgaatgaatg gggacatcgt gctcgtcggg aaggttatcg
     9541 tgtagataat acaccgacga ttggttccat tacttggtct actgcaggaa cttatggtca
     9601 tgttgcctgg gtgtcaaatg taatgggaga tcagattgag attgaggaat ataactatgg
     9661 ttatacagaa tcctataata aacgagttat aaaagcaaac acgatgacag gatttattca
     9721 ttttaaagat ttggatggcg gcagtgttgg gaatagtcaa tcctcagctt caacaggcgg
     9781 aactcattat tttaagacca agtctgctat taaaactgaa cccctagcta gcggaactgt
     9841 gattgattac tattatcctg gggagaaggt tcattatgat cagatacttg aaaaagacgg
     9901 ctataagtgg ttgagttata ctgcctataa tggaagctat cgttatgttc aattggaggc
     9961 tgtgaataaa aatcctctag gtaattctgt tctttcttca acaggtggaa ctcattattt
    10021 taagaccaag tctgctatca aaactgaacc cctagttagc ggaactgtga ttgattacta
    10081 ttatcctggg gagaaggttc attatgatca gatacttgaa aaagacggct acaagtggtt
    10141 gagttatact gcctataatg gaagtcgtcg ctatatacag ctagagggag tgacttcttc
    10201 acaaaattat cagaatcaat caggaaatat ctctagctat ggatccaata atagttcaac
    10261 tgtcggttgg aagaaaataa atggtagttg gtatcatttc aaatcaaatg gttctaaatc
    10321 aacaggatgg ctgaaagacg gttctagctg gtattatttg aaattatctg gtgaaatgca
    10381 gacaggatgg ttaaaggaaa atggctcgtg gtattatctg ggtagttcag gggcaatgaa
    10441 aacaggctgg taccaggtct ctggtaagtg gtattattct tactcttcag gcgccttagc
    10501 tgttaatacg acggtggatg gctacagagt aaacagtgat ggagaacgag tatagaaaat
    10561 tggagtagga gaaatttcct gctcttttct gtaagcagtt tccttgacat ttttcttatt
    10621 ttgcgctatc atatatacat ataatatatg ggagtctgtg tacagtctga gaggaagtgt
    10681 taaacttcga ccgcacctga tctgggtaat gccagcggag ggaacgatac ttagtctaat
    10741 tttgcacctt ttccatgtat ggtaaaggtt tttctttttt taaaaaggaa aacgagaaga
    10801 ggaggttctt atgaaagcaa gcattgcctt gcaagtttta cccctagtac aggggattga
    10861 tcggatagct gttattgatc aggtcattgc ttatctgcaa actcaagaag tgacgatggt
    10921 agtgacacca tttgaaacgg tcttggaagg ggagtttgat gagcttatgc gcattctaaa
    10981 agaagcgctg gaagtggcag ggcaggaggc agacaatgtc tttgccaatg tcaaaataaa
    11041 tgtaggagag attttaagta ttgatgagaa acttgagaag tatactgaga cgacacatta
    11101 gtctattggg ctttctcgga gtattgtcaa tctggcagtt agcaggtttt cttaaacttc
    11161 tccccaagtt tatcctgccg acacctcttg aaattctcca gccctttgtt cgtgacagag
    11221 aatttctctg gcaccatagc tgggcgacct tgagagtggc tttactgggg ctgattttgg
    11281 gagttttgat tgcctgtctt atggctgtac tcatggatag tttgacttgg ctcaatgacc
    11341 tgatttaccc tatgatggtg gtcattcaga ccattccgac cattgccata gctcctatcc
    11401 tggtcttgtg gctaggttat gggattttgc ccaagattgt cttgattatc ttaacgacaa
    11461 cctttcccat catcgtcagc attttggacg gttttaggca ttgcgacaag gatatgctga
    11521 ctttgtttag tctgatgcgg gccaagcctt ggcaaatcct gtggcatttt aaaatcccgg
    11581 ttagcctgcc ttacttttat gcaggtctga gggtcagtgt ctcctacgcc tttatcacaa
    11641 ctgtggtatc tgagtggttg ggaggttttg aaggtcttgg tgtttatatg attcagtcta
    11701 aaaaactgtt tcagtatgat accatgtttg ccattattat tctggtgtcg attatcagtc
    11761 ttttgggtat gaagctggtc gatattagtg aaaaatatgt tattaaatgg aaacgtactt
    11821 aaaatagagc gtttttgaaa aagaaaagag gaaatcaaaa tgaagaaaac atggaaagtg
    11881 tttttaacgc ttgtaacagc tcttgtagct gttgtgcttg tggcctgtgg tcaaggaact
    11941 gcttctaaag acaacaaaga ggcagaactt aagaaggttg actttatcct agactggaca
    12001 ccaaatacca accacacagg gctttatgtt gccaaggaaa aaggttattt caaagaagct
    12061 ggagtggatg ttgatttgaa attgccacca gaagaaagtt cttctgactt ggttatcaac
    12121 ggaaaggcac catttgcagt gtatttccaa gactacatgg ctaagaaatt ggaaaaagga
    12181 gcaggaatca ctgccgttgc agctattgtt gaacacaata catcaggaat catctctcgt
    12241 aaatctgata atgtaagcag tccaaaagac ttggttggta agaaatatgg gacatggaat
    12301 gacccaactg aacttgctat gttgaaaacc ttggtagaat ctcaaggtgg agactttgag
    12361 aaggttgaaa aagtaccaaa taacgactca aactcaatca caccgattgc caatggcgtc
    12421 tttgatactg cttggattta ctacggttgg gatggtatcc ttgctaaatc tcaaggtgta
    12481 gatgctaact tcatgtactt gaaagactat gtcaaggagt ttgactacta ttcaccagtt
    12541 atcatcgcaa acaacgacta tctgaaagat aacaaagaag aagctcgcaa agtcatccaa
    12601 gccatcaaaa aaggctacca atatgccatg gaacatccag aagaagctgc agatattctc
    12661 atcaagaatg cacctgaact caaggaaaaa cgtgactttg tcatcgaatc tcaaaaatac
    12721 ttgtcaaaag aatacgcaag cgacaaggaa aaatggggtc aatttgacgc agctcgctgg
    12781 aatgctttct acaaatggga taaagaaaat ggtatcctta aagaagactt gacagacaaa
    12841 ggcttcacca acgaatttgt gaaataatga cagaaattag actagagcac gtcagttatg
    12901 cctatggtca ggagaggatt ttagaggata tcaacctaca ggtgacttca ggcgaagtgg
    12961 tttccatcct aggcccaagt ggtgttggaa agaccaccct ctttaatcta atcgctggga
    13021 ttttagaagt tcagtcaggg agaattgtcc ttgatggtga agaaaatccc aaggggcgcg
    13081 tgagttatat gttgcaaaag gatctgctct tggagcacaa gacggtgctt ggaaatatca
    13141 ttctgcccct cttgattcaa aaggtggata aggcagaagc tatttcccga gcggataaaa
    13201 ttcttgcgac cttccagctg acagctgtaa gagacaagta tcctcatgaa cttagcggtg
    13261 ggatgcgcca gcgtgtagcc ttactccgga cctacctttt tgggcacaag ctctttctct
    13321 tagatgaggc ctttagcgcc ttggatgaga tgacaaagat ggaactccac gcttggtatc
    13381 ttgagattca caagcagttg cagctaacaa ccctgatcat cacgcatagt attgaggagg
    13441 ccctcaatct cagcgaccgc atctatatct tgaaaaatcg ccctgggcag attgtttcag
    13501 aaattaaact agattggtct gaagatgagg acaaggaagt ccaaaagatt gcctacaaac
    13561 gtcaaatctt ggcagaatta ggcttagata agtagaaaaa tagggagttg gtgaagatta
    13621 tcctttacca gcgccctttt tcttttaaaa atgagaaaat ttcggtataa tagtcaaaca
    13681 aggtcaaggt ttaaagagag aggtgggttt gttatgagat ttaaaaatac atcggatcat
    13741 attgaggcct acatcaaggc gattttagat caatctggta tcgtggagtt gcaacggagt
    13801 cagttggcag atacctttca ggttgttcct agtcagatta actacgtgat caagacacgc
    13861 tttacggaaa gtagaggcta cttggttgaa agtaagcgtg gtggtggagg ctacattcgt
    13921 ataggacgaa ttgagttttc tagtcatcat gaaatgctcc gggagctgct ttactcgatt
    13981 ggtgagcgag tcagtcaaga aatttatgag gatattctcc agcttttggt tgagcaggaa
    14041 ttgatgacca agcaggagat gaatttgcta gaatcagtag ctttggatcg cgttttagga
    14101 gaagaagctc cagttgttcg agcaaacatg ctacgtcaga tcatacaaga ggtagataga
    14161 aaagggaagt aagatgaact attcaaaagc attgaatgaa tgtatcgaaa gtgcctacat
    14221 ggttgctgga cattttggag ctcgttatct agagtcttgg cacttgttga ttgccatgtc
    14281 taatcacagt tatagtgtag caggggcaac tttaaatgat tatccgtatg agatggaccg
    14341 tttagaagag gtggctttgg aactgactga aacggactat agccaggatg aaacctttac
    14401 ggaattgccg ttctcccgtc gtttgcaggt tctttttgac gaagcagagt atgtagcgtc
    14461 agtggtccat gctaaggtgc tagggacaga gcacgtcctc tatgcgattt tgcatgatgg
    14521 caatgccttg gcgactcgta tcttggagag ggctggtttt tcttatgaag acaagaaaga
    14581 tcaggtcaag attgctgctc ttcgtcgaaa tttagaagaa cgggcaggct ggactcgtga
    14641 agatctcaag gctttacgcc aacgccatcg tacagtagct gacaagcaaa attctatggc
    14701 caatatgatg ggcatgccgc agactccgag tggtggtctc gaggattata cgcatgattt
    14761 gacagagcaa gcgcgttctg gcaagttaga accagtcatc ggtcgggaca aggaaatctc
    14821 acgtatgatt caaatcttga gccgtaagac taagaacaac cctgtcttgg ttggggatgc
    14881 tggtgtcggg aaaacagctc tggcccttgg tcttgcccag cgtattgcta gtggtgacgt
    14941 gcctgcggaa atggctaaga tgcgcgtgtt agaacttgat ttgatgaatg tcgttgcagg
    15001 gacacgcttc cgtggtgact ttgaagaacg catgaataat atcatcaagg atattgaaga
    15061 agatggccaa gtcatcctct ttatcgatga actccacacc atcatgggtt ctggtagcgg
    15121 gattgattcg actctggatg cggccaatat cttgaaacca gccttggcgc gtggaacttt
    15181 gagaacggtt ggtgccacca ctcaggaaga atatcaaaaa catatcgaaa aagatgcggc
    15241 tctttctcgt cgtttcgcta aagtgacgat tgaagaacca agtgtggcag atagtatgac
    15301 tattttacaa ggtttgaagg cgacttatga gaaacatcac cgtgtacaaa tcacagatga
    15361 agcggttgaa acagcggtta agatggctca tcgttattta accagtcgtc acttgccaga
    15421 ctctgctatc gacctcttgg acgaagcagc agcaacagtg caaaataagg caaagcatgt
    15481 aaaagcagac gattcagatt tgagtccagc tgacaaggcc ctgatggatg gcaagtggaa
    15541 acaggcagcc cagctaatct caaaagaaga ggaagtacct gtctacaaag acttggtgac
    15601 agagtctgat attttgacca ccttgagtcg cttgtcagga atcccagttc aaaagctgac
    15661 tcagactgac gctaagaaat atctaaatct tgaagcagaa ctccataaac gggttatcgg
    15721 tcaagatcaa gctgtttcaa gtattagtcg tgcgattcgc cgtaatcagt caggaattcg
    15781 cagtcacaag cgtccgatcg gttcctttat gttcctagga cccacaggtg tcggtaagac
    15841 cgagttggcc aaggctttgg cagaagttct ctttgatgac gaatcagccc ttatccgctt
    15901 tgatatgagt gagtatatgg agaaattcgc agccagccgt ctaaatggag ctcctccagg
    15961 ctatgtgggt tacgaagaag gtggggagtt gacagagaag gttcgcaata aaccctattc
    16021 cgttctcctc tttgatgagg tagagaaggc tcacccagat atctttaatg ttctcttgca
    16081 ggttctggat gacggtgtct tgacagatag caagggacgc aaggtcgatt tttcaaatac
    16141 cattatcatt atgacatcga acctaggtgc gactgccctt cgtgatgata agactgttgg
    16201 ttttggggct aaggatattc gttttgacca ggaaaatatg gaaaaacgca tgtttgaaga
    16261 actgaaaaaa gcttatagac cggaattcat caaccgtatt gatgagaagg tggtcttcca
    16321 tagcctatct agtgatcata tgcaggaagt ggtgaagatt atggtcaagc ctttagtggc
    16381 aagtttgact gaaaaaggca ttgacttgaa attacaagct tcagctctga aattgttagc
    16441 aaatcaagga tatgacccag agatgggagc tcgcccactt cgtagaaccc tgcaaacaga
    16501 agtggaggac aagttggcag aacttcttct caagggagat ttagtggcag gcagcacact
    16561 taagattggt gtcaaagcag gccagttaaa atttgatatt gcataaaaga ataaaagtat
    16621 cagcatctga ccataagtca cagtggagtg aaattcaatg aaaatcaaag agcaaactag
    16681 gcagctagcc gcaggttgct caaaacactg gtttgaggtt gcagatagag ctgacgtggt
    16741 ttgaagagat tttcgaagag tatgaaacta aaacctatag cttctaaacg atccgtggtt
    16801 ttcatcattc aacacaaaat tcatatgttt attaccctcc gtcgtatttg tcttagagcg
    16861 tgtgtagtag aaaaagagca gtcttatctg aaatttttat tctttcaaaa gagacctgtt
    16921 tcttttttgc atgtcaaatc cgttctagct ggtatttgaa aaatcaaact aatattcaat
    16981 gaaaatcaaa gaacaaacta ggaagctagc cgcaggttgc tcaaaacact gttttgaggt
    17041 tgtagataga gctgacgtgg tttgaagaga ttttcgaaga gtataagctg caagatgaat
    17101 gattttcttg tattgacgtt gttgttgaca aaaagtagcg gataaatgaa atccattcca
    17161 ttatcataga tgataggctg gtaggaaatt ttcaaatagc atacaggaaa tagatgtatg
    17221 gagttctggt agtagaaagg gagagagatg aacattttag ttgcagatga cgaggaaatg
    17281 attagagaag gaattgcagc atttctgaca gaagagggtt atcatgtcat tatggctaag
    17341 gatggacaag aggtcttgga aaaatttcaa gatctcccta tccatctcat ggtactggat
    17401 ttaatgatgc ctaggaagag tggttttgaa gtgttaaaag aaatcaatca aaagcacgat
    17461 attcctgtca tcgtcttgag tgctctggga gatgaaacta ctcagtcaca ggtatttgat
    17521 ctctatgctg atgatcatgt gacaaaacct ttttctttgg tactgcttgt caagcgtatt
    17581 aaggcgctta tcagacgtta ctacgtcata gaggatattt ggcgatatca ggatgtaaca
    17641 gtggatttta cctcttacaa agcacattat aaaaatgaag aaattgatct caaaccaaag
    17701 gaattactgg tactaaagtg tttgattcag cataaaaatc aagttttaag tagagagcag
    17761 atattggaag aaatttcaaa agatgtagct gatttacctt gtgatagggt cgttgatgtc
    17821 tatattcgta ctcttcgcaa aaaattagct ttagattgta tcgtgactgt gaaaaatgtt
    17881 gggtataaga ttagcttatg ataaaaaatc ctaaattatt aaccaagtct tttttaagaa
    17941 gttttgcaat tctaggtggt gttggtctag tcattcatat agctatttat ttgacctttc
    18001 ctttttatta tattcaactg gagggggaaa agtttaatga gagcgcaaga gtgtttacgg
    18061 agtatttaaa gactaagaca tctgatgaaa ttccaagctt actccagtct tattcaaagt
    18121 ccttgaccat atctgctcac cttaaaagag atattgtaga taagcggctc cctcttgtgc
    18181 atgacttgga tattaaagat ggaaagctat caaattatat cgtgatgtta gatatgtctg
    18241 ttagtacagc agatggtaaa caggtaaccg tgcaatttgt tcacggggtg gatgtctaca
    18301 aagaagcaaa gaatattttg cttttgtatc tcccatatac atttttggtt acaattgctt
    18361 tttcctttgt tttttcttat ttttatacta aacgcttgct caatcctctt ttttacattt
    18421 cagaagtgac tagtaaaatg caagatttgg atgacaatat tcgttttgat gaaagtagga
    18481 aagatgaagt tggtgaagtt ggtgaagttg gaaaacagat taatggtatg tatgagcact
    18541 tgttgaaggt tattcatgag ttggaaagtc gtaatgagca aattgtaaaa ttgcaaaatc
    18601 aaaaggtttc ctttgtccgc ggagcatcac atgagttgaa aaccccttta gccagtctta
    18661 gaattatcct agagaatatg cagcataata ttggagatta caaagatcat ccaaaatata
    18721 ttgcaaagag tataaataag attgaccaga tgagccactt attggaagaa gtactggagt
    18781 cttctaaatt ccaagagtgg acagagtgtc gtgagacttt gactgttaag ccagttttag
    18841 tagatatttt atcacgttat caagaattag ctcattcaat aggtgttaca attgaaaatc
    18901 aattgacaga tgctaccaga gtcgtcatga gtcttagggc attggataag gttttgacaa
    18961 acctgattag taatgcaatt aaatattcag ataaaaatgg gcgcgtaatc atatccgagc
    19021 aagatggcta tctctctatc aaaaatacat gtgcgcctct aagtgaccaa gaactagaac
    19081 atttatttga tatattctat cattctcaaa tcgtgacaga taaggatgaa agttccggtt
    19141 tgggtcttta cattgtgaat aatattttag aaagctatca aatggattat agttttctcc
    19201 cttatgaaca cggtatggaa tttaagatta gcttgtagac agattagttt tttattaaag
    19261 ttcatatagg gttaacataa gtgtgttatt ctttgtgtag ataaaagaaa ggatactaat
    19321 atggtattag cgattatttt agtaacattc cttattcgat tgattttttt aaagcgttcg
    19381 atagagaatg agaaacgaat ccttagcaat ggcgggaaag aatttggagt tgagaataca
    19441 aaacgattaa ctatggctca tattgttttt tatctctctt gcttggttga ggcaatggtg
    19501 cacaagacaa tttttgatgg catgggcatg gttggtttag tcttgcttat tttttctatg
    19561 ctgatgttaa tgttggtgat tcacttgttg ggagatattt ggacagtgaa gcttatgctt
    19621 gtcaataatc acaaatatgt agatcatatc ttgtttagga cagtaaaaca ccctaattac
    19681 tttttaaata ttttacctga gttgattggc ttgaccttgt tgagtcatgc ttatgtgact
    19741 tttgttttag tttttccagt ttatgcagtt attttgtatc gacgaatagc tgaagaggaa
    19801 aagctattac atgaagttat aatcccaaat ggaagcataa agagataaat acaaaattcg
    19861 atttatatac aggtcatatt gaagtgatat agtaaggtta aagaaaaaat atagaaggaa
    19921 ataaacatgt ttgcatcaaa aagcgaaaga aaagtacatt attcaattcg taaatttagt
    19981 attggagtag ctagtgtagt agttgctagt cttgttatgg gaagtgtggt tcatgcgaca
    20041 gagaacgagg gaagtaccca agtagccact tcttctaata tggcaaagac agaacatagg
    20101 aaagctgctg aacaattcga tgaatatata aacaaaatga tccaattaga tagaagaaaa
    20161 gatacccaaa atcgcacctt aatcataaag ttgagcgcaa ttaaaacgga gtatttgcgt
    20221 gaattaaatg ttttagaata taagtcgaaa gctgagttgc cgtcaaaaat aaaagcaaag
    20281 ttagacgcag cttttgatca gtttaaaaaa gatacattac caacagaacc aggaaaaaag
    20341 gtagcagaag ctaagaagaa ggttgaagaa gctgagaaga aggtagcaga agctaagaaa
    20401 aaagccaagg ctcaaaaaga agaagattac cgtaactacc caaccattac ttacaaaacg
    20461 cttgaacttg aaattgctga gttcgatgtg aaagttaaag aagcggagct tgaactagta
    20521 aaaaaggaag ctgacgaatc tcgaaacgag ggcacaatta accaagcaaa agcgaaagtt
    20581 gagagtgaaa aagctgaggc tacaaggtta aaaaaaatca agacagatcg tgaaaaagca
    20641 gaagaagaag aagctaaacg aagagcagat gctaagttga aggaagctgt tgaaaagaat
    20701 gtagcgactt cagagcaaga taaaccaaag gggcggagaa aacgaggagt tcctggagag
    20761 caagcaacac ctgataaaaa agaaaatgat gcgaagtctt cagattctag cgtaggtgaa
    20821 gaagctcttc caagcccatc cctgaaacca gaaaaaaagg ttgcagaagc tgagaagaag
    20881 gttgcagaag ctgagaaaaa agccaaggct caaaaagaag aagatcaccg taactaccca
    20941 accaatactt acaaaacgct tgaacttgaa attgctgagt ccgatgtgaa agttaaagaa
    21001 gcggagcttg aactagtaaa agaggaagct aaggaatctc gaaacgagga aaaagttaat
    21061 caagcaaaag cgaaagttga gagtaaaaaa gctgaggcta caaggttaga aaaaatcaag
    21121 acagatcgta aaaaagcaga agaagctaaa cgaaaagcag cagaagaaga taaagttaaa
    21181 gaaaaaccag ctgaacaacc acaaccagcg ccggctcctc aaccagaaaa accagctcca
    21241 aaaccagaaa aaccagctcc agctccaaaa ccagagaatc cagctgaaca accaaaagca
    21301 gaaaaaccag ctgatcaaca agcagaagaa gactatgctc gtagatcaga agaagaatat
    21361 aatcgcttga ctcaacagca accgccaaaa actgaaaaac cagcacaacc atctactcca
    21421 aaaacaggct ggaaacaaga aaacggtatg tggtacttct acaatactga tggttcaatg
    21481 gcgacaggat ggctccaaaa caatggctca tggtactacc tcaacagcaa tggcgctatg
    21541 gcgacaggat ggctccaaaa caatggttca tggtactatc taaacgctaa tggttcaatg
    21601 gcaacaggat ggctccaata caatggttca tggtactacc taaacgctaa tggttcaatg
    21661 gcgacaggat ggctccaata caatggttca tggtactacc taaacgctaa tggtgatatg
    21721 gcgacaggat ggctccaaaa caatggctca tggtactacc taaacgctaa tggtgatatg
    21781 gcgacaggat ggctccaata caatggttca tggtactacc taaacgctaa tggttcaatg
    21841 gtgacaggat ggctccaata caatggttca tggtactacc taaacgctaa cggttcaatg
    21901 gcaacagatt gggtgaaaga tggagatacc tggtactatc ttgaagcatc aggtgctatg
    21961 aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg ctcaggtgcc
    22021 cttgcagtca acacaactgt agatagctat agagtcaatg ccaatggtga atgggtaaac
    22081 taaacctaat ataactagtt aatactgact tcctgtaaga actctttaaa gtattcccta
    22141 caaataccat atcctttcag tagataatat acccttgtag gaagtttaga ttaaaaaata
    22201 actctgtaat ctctagctgg atttatagcg ctagagacta cggagttttt ttgatgagga
    22261 aagaatggcg gcattcaaga ggctctttaa gagagttacg ggttttaaac tattaagcct
    22321 tctccaattg caagagggct tcaatctctg ccagggtgct agcttgcgaa atggctccac
    22381 ggagtttggc agcgccagat gttccacgga gatagtgagg agcgaggccg cggaattcac
    22441 gaactgcgac gttttctcct ttgaggtcaa tcaatcgttt caagtgttcg taggcgatct
    22501 tcatcttgtc ttcaaaggtc aaatcaggta ggatttctcc tgtttcaaag taatggttga
    22561 tttggttgaa gaggtaagga tttcccatgg cagctcggcc aatcatgact gcgtcagcac
    22621 caacttcttc gatgcgttgc ttggcttctt ggacagtacg gatatcaccg ttggcgatga
    22681 atggaatctt ggttagagct tgggcaacct tgtaaagggt ctcaaggtct gcgtggccag
    22741 tatacatttg ttcacgggta cggccatgca tggcgagggc agaaacacct gcagcttcag
    22801 cagcgagagc attttctact gcaagagatg ggtccgccca gccggtacgc attttgacag
    22861 taagtgggat atcaaggaca gactggacct tgttgatgat agagtagatc ttgtcaggat
    22921 ccttgagcca catagcacca gcttcgttct tcacgatttt gttgacaggg cagcccatgt
    22981 tgatatcgac gatatcggtc ttggtgtttt cttggataaa ttctgctgcg cgtgctaggc
    23041 tgtcttcatc gctaccaaaa agttggatag agacagggtt ttcgccctca tcgatatgaa
    23101 gcatgtgcag ggttttttcg ttgttgtatt ggattccctt gtcagagacc atttccatta
    23161 caacgagtcc agctccgagc tcttttgcga tggtacgaaa ggctgagttg gtcacgccag
    23221 ccataggcgc taaaacggta cgattgggaa tctcaacatt gccaatcata aaaggtgtat
    23281 taagatttgt cacgaatgag ttcctccagg tccttttcat caaagttgta agtagtttgg
    23341 cagaattgac aagtgatttc taccccgtgg tcttcctctt tcatttcctg taagtctgag
    23401 cttggaaggc tggcaagagc gttcataaag cgttcatggc tacagtcaca ttggaaacgg
    23461 atttcttctt cagaaagacg cttgtaggct tcgtccccgt agatagcctt gaggagggct
    23521 tcgatatggt cgtcgctttc gagaagagta gagatagctg gcatttcttg gatgcgtttt
    23581 tcaaagcgag caatctcttc tttcttggct cctggcaaga cttgaactag gaaaccacct
    23641 gcaatcttga ccttgtcttc ctcgtccaaa aggacattga ggccgaccgc tgaaggcgtt
    23701 tgttggcttt cagtaaggta aaaggcaagg tcttcaccga tttctccaga gatgagggga
    23761 gttatagagt tgtaaggatt tccagtaccg tagtctgtga taacgaggaa ttgaccattt
    23821 ccaacaaaag gtccgactag gacttcacca gtcgcagtct ttttgatgtc aacaccagga
    23881 ttttgaacgt agcctttgac gttccccttg gtatcagcga cggtgataat agcacctaga
    23941 gagctagatc ccaacacctt aactgtaagt ttggtatttc ctttttcatt ggctgcgaga
    24001 atctggctgg cgataagagt tcgaccaagc gctacagttg agctagcttg ggtttgatgt
    24061 ttttcttgag cagtgcggac ggtttcagtg ctatcaagga caaaagcacg aaaggctccg
    24121 ctttctgata tagttttaat aattttatcc atagctacta ttttagcata aaaatgccca
    24181 aagggggagc cgtgtgttta ctgattttca ggataatgga ccaggaaatc agcatgaaaa
    24241 taaaaagaga aacagattat tttagcattt gtaagattta tgctatgctt aaggtagaaa
    24301 atgaaagggg taacaaatgt atttaggaga tttgatggag aaagccgagt gtggtcaatt
    24361 ttcaatcctt tcctttctat tacaagagtc tcagacgacc gtcaaggctg taatggaaga
    24421 aacaggattt tcaaaagcaa ccctaaccaa atatgtcacc ctgctcaatg acaaggcttt
    24481 ggatagtggc ttagagctgg ctattcactc agaagatgaa aatctgcgtc tgtctatcgg
    24541 tgcagctacc aaggggagag atattcggag cttgtttttg gagagtgctg ttaaatacca
    24601 gattttggtt tatcttctct accaccaaca gtttttagcc catcagctgg ctcaagaatt
    24661 ggtgattagc gaggctacgc ttggtcgtca cttggctggt ttaaatcaga ttttgtcaga
    24721 atttgattta tccatccaaa atggacgttg gcgaggtcca gagcatcaga ttcgctattt
    24781 ctatttctgt cttttccgaa aggtctggtc gagtcaggaa tgggaaggtc acatgcagaa
    24841 accagagaga aaacaggaga ttgccaattt agaggaaatc tgcggtgcaa gtttgtctgt
    24901 ggggcagaaa ttggacttgg ttctctgggc tcacatcagt caacaacgtc ttcgggtcaa
    24961 tgcttgtcag tttcaagtca tagaagagaa aatgcgaggg tattttgaca atatctttta
    25021 tcttcgtttg ctgagaaagg ttccgtcctt ttttgctggg caacatattc cactaggagt
    25081 tgaggatggt gagatgatga tattcttctc ttttctccta tctcatcgca ttcttcctct
    25141 tcatactatg gagtatattc ttggttttgg agggcagttg gcagatttac tgacgcaatt
    25201 gattcaagaa atgaagaagg aggaactatt gggggattat acagaggacc atgtcaccta
    25261 tgaactcagt cagctttgtg ctcaagtcta tctctataag ggctatattt tacaggatcg
    25321 ctacaagtac cagttagaga atcgtcatcc atatttactg atggaacatg attttaaaga
    25381 gacagcagag gagatttttc atgctctacc tacttttcaa caggggacag atttagataa
    25441 gaagattctc tgggaatggc tccagttaat cgaatatatg gctgaaaacg gtggccagca
    25501 tatgcggatt ggtctggatt tgacatctgg ttttcttgtc ttttcaagga tggcagccat
    25561 tttgaaacgg tatttggaat acaatcgttt tattaccatt gaagcctatg accctagtcg
    25621 gcattatgat ttgctggtta ccaataaccc tattcataag aaggaacaga caccagtcta
    25681 ttatttaaaa aatgacttgg atatggaaga tttggcaggg attcgtcagt tattattcac
    25741 ttaaaaggct tggttaatcc aggtcttttt tgtgaaattc acacaatctc ctcacatttt
    25801 tttaaaaatt aaaaaaagtt gataaacaag aaagcgcttt attttgtata ctagtaagtg
    25861 taaagaggaa acacctcaag atctttatca ggaggacagt acatgtcaca agaaaaatac
    25921 atcatggcca ttgaccaggg aactacaagt tctcgtgcca tcattttcaa caaaaaaggg
    25981 gaaaaggtta gctcgagtca aaaagagttt acccagattt tccctcaggc aggttgggtt
    26041 gagcacaatg ccaatgaaat ttggaactct gttcagtcag ttattgcggg tgctttcatc
    26101 gaaagtggtg tcaagccaaa tcaaatcgag gcaatcggga ttaccaacca acgtgaaaca
    26161 acggttgtct gggataagaa aacaggactt cctatctaca atgctatcgt ttggcagtca
    26221 cgccagacag cacctttggc tgagcaacta aaaagccaag gttatgtgga aaaattccat
    26281 gaaaagactg gtttgattat tgatgcttac ttctctgcta ccaaggttcg ttggattttg
    26341 gatcatgtag aaggtgctca agagcgagca gaaaaagggg aattgctctt tggtactatc
    26401 gatacttggt tggtttggaa attgactgac ggtgcggctc acgtgactga ctactcaaat
    26461 gcagctcgta ccatgcttta taacattaaa gaactcaaat gggatgatga gattttggaa
    26521 atccttaaca ttccgaaggc tatacttcca gaagttcgtt ctaactccga aatctacggc
    26581 aagacagctc cattccattt ctacggtgga gaggtgccaa tctcaggtat ggctggggac
    26641 caacaagcag ccctctttgg acagttggct tttgagccag gtatgattaa gaatacttat
    26701 ggaacaggct ctttcatcat catgaatact ggggaagaga tgcagttgtc tgaaaacaac
    26761 ctcttgacaa ccattggtta cggaatcaac ggtaaggttt attatgcctt ggaaggttct
    26821 atcttcatcg caggaagtgc tattcagtgg cttcgtgacg gtcttcgcat ggttgaaaat
    26881 tcaccagaat ctgaaaaata cgctcgtgat tctcacaaca acgatgaagt ttatgtcgtt
    26941 ccagccttta caggtctagg cgctccatac tggaaccaaa atgctcgtgg ttccgtcttt
    27001 ggtttgactc gtggaacaag caaagaagac tttatcaagg cgactttgca atctattgct
    27061 tatcaagtgc gtgacatcat cgacaccatg caagtggata ctcagaccgc cattcaagta
    27121 ttgaaggtgg atggtggtgc agccatgaac aacttcctca tgcagttcca ggcggatatt
    27181 ttaggcattg atattgcacg tgctaaaaac ttggaaacaa cagctctagg agcggccttc
    27241 ctagcaggtt tgtcagtagg gtactggaaa gacttggacg agttgaaact cttgaacgag
    27301 acaggagaac tctttgagcc atctatgaac gaatctcgca aggaacaact ctacaagggc
    27361 tggaagaagg ctgtgaaagc aactcaagtc tttgcggaag tagacgacta atactggcag
    27421 aataaagcga tttatttaga aagtgtgtaa atatggaatt ttcaaagaaa acacgtgaat
    27481 tgtcaattaa aaaaatgcag gaacgcaccc tggacctctt gattatcggt ggaggaatca
    27541 caggagctgg tgtagccttg caggcggcag ctagcggtct tgagactggt ttgattgaaa
    27601 tgcaagactt tgcagaagga acatctagtc gttcaacaaa attggttcac ggaggacttc
    27661 gttacctcaa acaatttgac gtagaagtgg tctcagatac ggtttctgaa cgtgcagtgg
    27721 ttcaacaaat cgctccacac attccaaaac cagatccaat gctcttacca gtttacgatg
    27781 aagatggagc aacctttagc ctcttccgtc ttaaagtagc catggacttg tacgacctct
    27841 tggcaggtgt tagcaacaca ccaactgcga acaaggtttt gagcaagaaa gaagtcttgg
    27901 aacgccagcc aaacttgaag aaggaaggct tggtaggagg tggagtgtat cttgacttcc
    27961 gtaacaacga tgcgcgtctc gtgattgaaa acatcaaacg tgccaaccaa gacggtgccc
    28021 tcattgccaa ccacgtgaag gcagaaggct tcctctttga cgaaagtggc aagattacag
    28081 gtgttgtagc tcgtgatctc ttgacagacc aagtatttga aatcaaggcc cgtctggtta
    28141 ttaatacaac aggtccttgg agtgataaag tacgtaattt gtctaataag ggaacgcaat
    28201 tctcacaaat gcgcccaact aagggagttc acttggtagt ggattcaagc aaaatcaagg
    28261 tttcacagcc agtttacttc gacacaggtt tgggtgacgg tcgtatggtc tttgttctcc
    28321 cacgtgaaaa caagacttac tttggtacaa ctgatacaga ctacacaggt gatttggagc
    28381 atccaaaagt aactcaagaa gatgtagatt atctacttgg cattgtcaac aaccgctttc
    28441 cagaatccaa catcaccatt gatgatatcg aaagcagctg ggcaggtctt cgtccattga
    28501 ttgcagggaa cagtgcctct gactataatg gtggaaataa cggtaccatc agtgatgaaa
    28561 gctttgacaa cttgattgcg actgttgaat cttatctctc caaagaaaaa acacgtgaag
    28621 atgttgagtc tgctgtcagc aagcttgaaa gtagcacatc tgagaaatat ttggatccat
    28681 ctgcagtttc tcgtgggtct agcttggacc gtgatgacaa tggtcttttg actcttgctg
    28741 gcggtaaaat cacagactac cgtaagatgg ctgaaggagc tatggagcgc gtggttgaca
    28801 tcctcaaagc agaatttgac cgtagcttta aattgatcaa ttctaaaact taccctgttt
    28861 caggtggaga attgaaccca gcaaatgtgg attcagaaat cgaagccttt gcgcaacttg
    28921 gagtatcacg tggtttggat agcaaggaag ctcactatct ggcaaatctt tacggttcaa
    28981 atgcaccgaa agtctttgca cttgctcaca gcttggaaca agcgccagga ctcagcttgg
    29041 cagatacttt gtcccttcac tatgcaatgc gcaacgagtt ggctcttagc ccagttgact
    29101 tccttcttcg tcgtaccaac catatgctct ttatgcgtga tagcttggat agcatcgttg
    29161 agccagtttt ggatgaaatg ggacgattct atgactggac agaagaagaa aaagcaactt
    29221 accgtgctga tgtcgaagca gctctcgcta acaacgattt agcagaatta aaaaattaag
    29281 aaaaaataaa agaggtggag ggcagcattc cttgtcgccc gccccttctt tttaatggag
    29341 acagaaagat gatgaatgaa ttatttggag aatttctagg gactttaatc ctgattcttc
    29401 taggaaatgg tgttgttgca ggtgtggttc ttcctaaaac caagagcaat agctcaggtt
    29461 ggattgtgat tactatgggt tgggggattg cagttgcggt tgcagtcttt gtatctggca
    29521 agctcagtcc agctcattta aacccagctg tgaccatcgg tgtggcctta aaaggtggtt
    29581 tgccttgggc ttccgttttg ccttatatct tagcccagtt cgcaggggcc atgctgggtc
    29641 agattttggt ttggttgcaa ttcaaacctc actatgaggc agaagaaaat gcaggcaata
    29701 tcctggcaac cttcagtact ggaccagcca tcaaggatac tgtatcaaac ttgattagcg
    29761 aaatccttgg aacctttgtt ttggtgttga caatctttgc tttgggtctt tacgattttc
    29821 aggcaggtat cggaaccttt gcagtgggaa ctttgattgt cggtatcggt ctatcactag
    29881 gtgggacaac aggttatgcc ttgaacccag ctcgtgacct tggacctcgt atcatgcaca
    29941 gcatcttgcc aattccaaac aagggagacg gagactggtc ttacgcttgg attcctgttg
    30001 taggccctgt tatcggagca gccttggcag tgcttgtatt ctcacttttc tagtttatac
    30061 tcttcgaaaa tcaaattcaa accacgtcag cgtcgcctta ccgtactcaa gtacagcttg
    30121 cggctagctt cctagtttgc tctttgattt tcattgagta ttagaaaaca attatgttga
    30181 tagagcttgg gcaagagccc aatttcagca aaaaatgaag taaatcttct cataataaaa
    30241 cgcatcatat caagcacgaa aattccacga ggtcaactac agtcagaaag ctgaacaaca
    30301 agccaaaacg cccaaaaaag gcggcaaaaa gcaagcacct gcaagcaacg tgccgaaatg
    30361 gtcaaatcct gattatgtca acgaattaga cccaaaaatc gttgatatgc tagtagaatt
    30421 tcacaagtca caaggcactt tggaaactcc cgaggcgcaa gcagaaatcg cccaaaaacg
    30481 tgaagaaatc gagcaaagga gagctgagct tgagggtaaa aaacaagagc ttttgaaccg
    30541 cttgaacaaa tagagtttcg caagtattat gcttacaaat tacttgagca attaactaaa
    30601 atataaaccc tgcctttata tctaggcagg gtttatattt tagaaattca cataggttgt
    30661 tacggttttt acatacccag tatagtttga gtttctatag tattcagtga taaacttcca
    30721 ttttctttga gcaacatgga tataagtact tgttatgtag tatggatatg ggctttgtga
    30781 atccaagtaa gactgataag cttgtatacc aaaatatgct ccaccaatta ttgcacccca
    30841 tggccccccc aataaagcac ctatcctacc aatcatataa ctgattccag caccagtcat
    30901 gaagttagcg aatgtgttag cttgtttatt cccatgtatt gtgttgacgt aattccaaac
    30961 attaggatcg tatgatctaa aagatatatt taggtcgatt tcattctttt gataagccat
    31021 ataaaatgcc ccattgatat agacgccgtc agcacgtcgt tcaatagtgt ctacacttcc
    31081 atctggattg acaacctcaa gaacttcatc gcttaaaata tttacttgcg tatctccgaa
    31141 ccgcactgat gagccattct caaactgagc ctcaccagat acaactttag agtttgccga
    31201 taagctatca tcagcaaaaa caaacaagcg acggggaaat gctagacata cagaaaacag
    31261 acataactag caaacacatg catttaaaca tcttagacat aacggaaact cctttgtatt
    31321 tttgattttt ttcaactttt attatacaat aaaaccaaat aaaaagaaag cggtaacaat
    31381 atgcttaatg cgaaaatttt ttatatattt ttatgtttga tcgttatcga aactacaggc
    31441 ttgttgttgt tgaaaagagg tctcgaaatg ggttatttag acacagaagc tattatcctc
    31501 gcagtttttt catttgcttt ttacaaccta tgttcattcg cttgggtctg ctctacaata
    31561 aaaaacaat
//
LOCUS       DAVLYV010000016        26729 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLYV010000016 DAVLYV010000000
VERSION     DAVLYV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26729
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.016"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            120..668
                     /locus_tag="VYO70_000759"
     CDS             120..668
                     /locus_tag="VYO70_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1713027.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            778..1680
                     /locus_tag="VYO70_000760"
     CDS             778..1680
                     /locus_tag="VYO70_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1713028.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKRSS"
     gene            complement(1701..2975)
                     /gene="serS"
                     /locus_tag="VYO70_000761"
     CDS             complement(1701..2975)
                     /gene="serS"
                     /locus_tag="VYO70_000761"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1713029.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(3189..3560)
                     /locus_tag="VYO70_000762"
     CDS             complement(3189..3560)
                     /locus_tag="VYO70_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1713030.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIARKKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            complement(3563..4927)
                     /locus_tag="VYO70_000763"
     CDS             complement(3563..4927)
                     /locus_tag="VYO70_000763"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1713031.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            5252..6037
                     /locus_tag="VYO70_000764"
     CDS             5252..6037
                     /locus_tag="VYO70_000764"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1713032.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            6131..6565
                     /locus_tag="VYO70_000765"
     CDS             6131..6565
                     /locus_tag="VYO70_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1713033.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            6565..7539
                     /locus_tag="VYO70_000766"
     CDS             6565..7539
                     /locus_tag="VYO70_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1713034.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            7599..7823
                     /locus_tag="VYO70_000767"
     CDS             7599..7823
                     /locus_tag="VYO70_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1713035.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            7942..8916
                     /gene="fabK"
                     /locus_tag="VYO70_000768"
     CDS             7942..8916
                     /gene="fabK"
                     /locus_tag="VYO70_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1713036.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            8909..9829
                     /gene="fabD"
                     /locus_tag="VYO70_000769"
     CDS             8909..9829
                     /gene="fabD"
                     /locus_tag="VYO70_000769"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1713037.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            9863..10594
                     /gene="fabG"
                     /locus_tag="VYO70_000770"
     CDS             9863..10594
                     /gene="fabG"
                     /locus_tag="VYO70_000770"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1713038.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            10616..11851
                     /gene="fabF"
                     /locus_tag="VYO70_000771"
     CDS             10616..11851
                     /gene="fabF"
                     /locus_tag="VYO70_000771"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1713039.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            11854..12339
                     /locus_tag="VYO70_000772"
     CDS             11854..12339
                     /locus_tag="VYO70_000772"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1713040.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            12336..12758
                     /gene="fabZ"
                     /locus_tag="VYO70_000773"
     CDS             12336..12758
                     /gene="fabZ"
                     /locus_tag="VYO70_000773"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1713041.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            12770..14137
                     /gene="accC"
                     /locus_tag="VYO70_000774"
     CDS             12770..14137
                     /gene="accC"
                     /locus_tag="VYO70_000774"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1713042.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            14174..15040
                     /locus_tag="VYO70_000775"
     CDS             14174..15040
                     /locus_tag="VYO70_000775"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1713043.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            15037..15804
                     /locus_tag="VYO70_000776"
     CDS             15037..15804
                     /locus_tag="VYO70_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1713044.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            15963..16148
                     /gene="briC"
                     /locus_tag="VYO70_000777"
     CDS             15963..16148
                     /gene="briC"
                     /locus_tag="VYO70_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1713045.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRAG"
     gene            16511..16888
                     /locus_tag="VYO70_000778"
     CDS             16511..16888
                     /locus_tag="VYO70_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1713046.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYLMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(16937..17359)
                     /gene="nusB"
                     /locus_tag="VYO70_000779"
     CDS             complement(16937..17359)
                     /gene="nusB"
                     /locus_tag="VYO70_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1713047.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(17352..17741)
                     /locus_tag="VYO70_000780"
     CDS             complement(17352..17741)
                     /locus_tag="VYO70_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1713048.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            complement(17763..18323)
                     /gene="efp"
                     /locus_tag="VYO70_000781"
     CDS             complement(17763..18323)
                     /gene="efp"
                     /locus_tag="VYO70_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1713049.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            complement(18452..19894)
                     /gene="gatB"
                     /locus_tag="VYO70_000782"
     CDS             complement(18452..19894)
                     /gene="gatB"
                     /locus_tag="VYO70_000782"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1713050.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            complement(19894..21360)
                     /gene="gatA"
                     /locus_tag="VYO70_000783"
     CDS             complement(19894..21360)
                     /gene="gatA"
                     /locus_tag="VYO70_000783"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1713051.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            complement(21360..21662)
                     /gene="gatC"
                     /locus_tag="VYO70_000784"
     CDS             complement(21360..21662)
                     /gene="gatC"
                     /locus_tag="VYO70_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1713052.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            21875..23419
                     /locus_tag="VYO70_000785"
     CDS             21875..23419
                     /locus_tag="VYO70_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1713053.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVSGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            23674..23872
                     /locus_tag="VYO70_000786"
                     /pseudo
     CDS             23674..23872
                     /locus_tag="VYO70_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
     gene            23983..24159
                     /locus_tag="VYO70_000787"
     CDS             23983..24159
                     /locus_tag="VYO70_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1713054.1"
                     /translation="MDLDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     NGMWRAENVTQDLI"
     gene            24275..24463
                     /locus_tag="VYO70_000788"
     CDS             24275..24463
                     /locus_tag="VYO70_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1713055.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            24620..24985
                     /locus_tag="VYO70_000789"
     CDS             24620..24985
                     /locus_tag="VYO70_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1713056.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            24988..26655
                     /locus_tag="VYO70_000790"
     CDS             24988..26655
                     /locus_tag="VYO70_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1713057.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALRD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
BASE COUNT         7677 a         5069 c         6181 g         7802 t
ORIGIN      
        1 taatataaca acgaaaatct tatgagagaa taaagcttta tcttagataa acttaggaat
       61 caggatttta taagattttc caataatatt agtgtcaata ataagaaaag aggtatctta
      121 tgacaacatt tactatccat acagtagagt cagcaccagc agaagtgaaa gaaattcttg
      181 aaacagtaga aaaagacaac aatggctata ttcccaacct aatcggtctc ttggccaatg
      241 ccccgactgt tttagaagcc taccaaattg tctcatctat ccaccgtcgc aacagcctga
      301 cacccgttga gcgtgaagtg gtgcaaatca cggcagccgt gaccaatggt tgtgccttct
      361 gtgtcgcagg tcacacagcc ttttccataa aacaaatcca gatgaatgat gacttgattc
      421 aagctcttcg caatcgtact ccaattgaaa cagatcctaa attggatacc ctagctaagt
      481 ttaccttggc agttatcaat accaagggtc gtgtaggaga tgaagccttg tctgagtttt
      541 tagaagctgg ctacactcaa caaaatgcct tggatgtggt ttttggtgtc agcctagcaa
      601 tcctctgtaa ctatgccaac aacttagcta atacaccaat taatccagaa ttgcaacctt
      661 atgcctaatt catatctgag taaaatgaag tctgaaataa gcggacttcg tttttttaaa
      721 tcctcattta agcgctttta tgttatactg aaactaacat gattattgga ggttaggatg
      781 aaaaaactcc ccttagtatt ttcaggttgt ttgctaggtt tggcaggagc tggaaatctt
      841 attttagata cgttgccggt tctatcccat ctttttagtc tgactggttt ggttttatgg
      901 atttactttc taattctgca tctctttaat tggaaagaaa ccaagcaaga attgaccaag
      961 ccccctcttt tgtcaggaat ggcaaccttt cctatggctg ggatgatttt atcgacctat
     1021 gtctttcgcg tattctctta tcttcctttg gtagcacaag ggatttggtg gttttcattt
     1081 ctcttggatt tgaccttgat tgctggtttt accatcaagt ttgcttgtcc agggcggagg
     1141 gttcatgcca ctccaagttg gacggttctc tatgtgggga tagcagtggc tgccttgacc
     1201 tatcctctgg taggtattat cgaaattgcc tatgcgacct tgagttttgg ttttctcttg
     1261 accttctatc tctatcccct tatttatagc gatttaaaga aacatccact cccactagcc
     1321 ttgcttggac aagaaggaat ctactgtgct cctttctctc tactcttggc ttctctagtt
     1381 cgagtaggag gaaccagcct gccgacttgg gtcttgattg tcatgatttt ggcttctcaa
     1441 tccttctttt tctttgtttt aactcgtctg cccaacattt taaaacaagg ttttcaacca
     1501 gccttctcag ccctcacctt cccaaccatt atcacagcga cctcgctcaa gatggctcag
     1561 ggaattttga aacttccatt tctggattac ctggtattgg ctgaaaccat tatatgccta
     1621 actattttat tctttgtact aggtgcttat ctgatttggt tacgaaaaag gtctagctag
     1681 aaatagctaa accttatttt ttatggtttg atgacttcag ctccacccat gtatggacga
     1741 agtgcttctg ggatggtcac agaaccatct tcattttggt aattttcaag aattgcagcc
     1801 actgtacgtc caactgcaag tccagaaccg ttcaaggtat gaaggagttt caccttgcca
     1861 tctgcttcat cacggtaacg gatttgggca cgacgggctt ggaaatcttc tgtgtttgaa
     1921 cagcttgaga tttcacggta attgttttgt gctggaatcc acacttccaa gtcgtaagtc
     1981 ttcgtagctg agaagcccat atctccagta gagagagcaa cgacacggta tggaaggttg
     2041 agtttttgaa gaatgttttc agcgttggct gtcatttttt ccaattcttc gtaagattct
     2101 tctggtttgg caaatttgac catttcaacc ttgtggaatt ggtgcaaacg gatcaagcca
     2161 cgcgtatcac gaccggcaga accagcctca gaacggaatg acggactcat agcagtgaag
     2221 tagattggaa gatctttgcc gtctaagatt tcatcacggt agtagtttgt cagaggaact
     2281 tcagcagttg gaatcaagac aaagttggta tcgctgagtt caaaagtatc ttccttaaat
     2341 tttggatact gaccagtacc aaacatagaa tcatggttga ctatgtaagg tgtgatgact
     2401 tcagtatagc cttcttttcc atgttcatcc aacataaagt tgtagatagc acgttccaaa
     2461 cgagcaccga ggcctttata gaagaggaag cgagcgcctg ttaccttacc accgcgttcc
     2521 cagtcaagga taccaaggtc ttcaccgaga tcccagtgag ctttaggttc gaagtcaaac
     2581 tcgcgtggag taccccaacg gcgaacttcc acattgtcgt cttcgtcagt cccaacagga
     2641 acgctgtcag ctgggatatt tggaagagtc gttgtaaatt ctgtcaattt agcatcgatt
     2701 tctgccaatt cagcatccaa ggctttaacc tcagcagata gattttgcat ggcagcaatc
     2761 ttgtcatctg tattttcctt gttgcgctta gcttgggcaa tctcagcaga aactgtgtta
     2821 cgttctgctt tgagagtttc aaccttgacc aagatgttac gacgtttagc atcgatttct
     2881 ttcatttcat tcaagacagc agcatctaca ccacgtgtag ctaatttttc tgcgacagct
     2941 tcaaaatctg tacgaatacg tttgatatct aacataagaa ctcctttatg aaaaaagcac
     3001 gcctgacaaa acgttggagt ggcagggcca cggttccatc caacttcaca ggtgtgcact
     3061 tgattgtgta tgtaattgtt attaacggta gaatttcacc tatccctcct atctgctcgc
     3121 agtacccgca gactttctga aagaagaaga taacctactt atccgttgct atgattatac
     3181 taaagtttct acttttttgc aaatagattt ttaaattttt ggctaattgt ctgaatcagg
     3241 gtcggaagtt tgacgacctt gtcattgcct agttttttgc gtgcaatttt gagaatggca
     3301 cctgagtctt ttgaagcaaa gaggaatttt cctttgtctg taaagacttc gaagtggcgg
     3361 ctgattttgc gtccagtgac attggctcca atctgattga tatggctcca aggaatctgg
     3421 ataaattgtt cgacattgac atctgggtaa aattccaaag cctgatctcc gacaaggaat
     3481 ttcccaactt tcccagcgat agagaggtag gaagtgcctg tcgtactgag gagtactgtt
     3541 ttgttaagtg attgggccat gcttagtctt ccttactttc tccaaaaaag gcattgtaga
     3601 gggctttaat tgctgctttc tcttggtctt tattgacaac aaacataata gaaacttcac
     3661 tagaaccttg agacatcatc tggatgttga ttttgttttc agatagagcg cgtgtcgcag
     3721 tagcagtcac tccgatgtgg ctcttcattt tttcaccaac aatcataatg atagaaaggt
     3781 cgtgttcgat ttctgcatga tctactttag ccttttgaac caactgacgc aggatttctt
     3841 cttccttgat gggagttagt tggcgagaac ggagaatgat agaaagatcg tcgatacctg
     3901 ttggcatatg ttcccaaccg atgttaagtt cttccaggat ttgcagaacc ttgcggccaa
     3961 atccaacctc acggttcatg aggtatttcg acatgttaat gctgacaaag cctgagtcac
     4021 cagcaattcc cacaactgga aattcatcat tactgtgttt tagaacgata cgagtacctg
     4081 gatggtcagg gttgttggta ttcttgataa ccagaggaat ttttccacgg taggcaggaa
     4141 gaagagcctc gtcatgaagg actgagaagc ctgcataggc caactcgcgc atttcacggt
     4201 aggtcaactc aggaatcgag tgtggttggt ggataatacc agggtgggct gcaaagatac
     4261 catcaacgtc cgtaaagttt tcatagaggt cagctttgac accagcagca atgatagaac
     4321 ctgtaatatc agaacctcca cgtgagaaag tacagatttg attttcctta gtgacaccaa
     4381 agaaaccagg aatgacaagg acttcattgg tgtttgtcaa ttcttcaatc ttgtcataac
     4441 ttgatggaat gatgcgagcg tgaccaggtt cacttgtgac cacaatccca gcttctctag
     4501 ggtgcatata gcgtgcatcg ataccatttt ggttaaagta ggcagcaatc aatttggcat
     4561 tgttattttc accggctgct aggaaagtat cgtagagaaa ttcattttct tcaataggaa
     4621 gagtggccaa ggcgtgaata cttttagaaa ttttttctag cacagctggt tttagtccca
     4681 attcactaac catagcagca tagcggtcga taatccagct ttggctcttg ctaatatcgt
     4741 tacccgcaac atagtcgcgg tagtatttaa tcagggcatc cgtaacctta gtatcttcag
     4801 cattgcgttt accaggcgca gaaacgacta caaaacgacg ctctgaatcg cttttgacga
     4861 tgtttaaaac tttttctaat tgactagcag aggcaagaga actacctcca aatttaacaa
     4921 ctttcataag aactcctaaa gtaagtattt tatacgatta tagcagaaag aaaggctttt
     4981 ttcaatgaag aaaataagat gctttctagc ctaaagcgag cctttcttat cggctgagac
     5041 actttcagtc gattttttct tgctttctgc tcctaaaaaa gatatacttt gacagtgaaa
     5101 taatttaaca ctcaatgaaa atcaaagagc aaactaggaa gctagccgaa gtcagctcaa
     5161 agcactgctt tgaggttgca gatggaagct gacgcggttt gaagagattt tcgaagagta
     5221 taaactgctt ataaaataaa aaggagccct gatggaacac attatttatc agcttgaaga
     5281 ggacttggca atccttacct tgaaccgtcc tgaggtcgca aatggttttc atattcccat
     5341 gtgtgaggag attttagaag ctctgacttt ggcagaagaa aatccagctg tgcattttat
     5401 cttaatcaat gccaatggaa aggtcttctc agttggggga gatttggtag agatgaagcg
     5461 ggcagtggat gaggatgata ttccatcatt gacaaaaatc gcagagttgg tcaatacgat
     5521 ttcttataaa atcaagcaaa tagccaaacc tgttttaatg gaagttgacg gtgctgttgc
     5581 aggtgccgca gcgaatatgg ctgttgcggc agatttctgt ttggcgacgg ataaggctaa
     5641 gtttatccaa gcctttgttg gtgttggttt ggctccagat gcagggggaa ttcatctctt
     5701 gagccgcagt atcggtgtga cgcgtgctgc tcaattagct atgacaggag aggctttaac
     5761 ggcagaaaaa gccctagaat gggggttggt ttatcgcgtt tctgaagctg aaaaacttga
     5821 aaagacgaga gaacagcttc ttaaaaaatt aagacgtgct tcaagtaatt cctatgctgc
     5881 cattaagaag ttggtttggg agagccaatt taaagactgg cagggttacg ctactttaga
     5941 actgaaccta cagaaatcct tagctcaaac agaggatttc aaagaaggag tgcgagctca
     6001 ttcggagaga agaagaccta aatttacagg aaaataaaaa atacttgcat cattctttga
     6061 attttgatat aattctttct gtcaaatgtt ttgattgtaa aagttttttg aagaaggagg
     6121 gatatgaaaa atggactacc aacgaatcaa tgaatattta acatctatat ttaacaatgt
     6181 ccttgtaatt gaggaagtga acttgagagg tagtcgtttt aaggatatct ccatcaaaga
     6241 aatgcatacg attgatgtca tcggtaaggc tccagacgtg actccaagtc aagtgtcaaa
     6301 agagttgatg gtaactcttg gaactgttac gacaagtttg aacaatttag agcgtaaggg
     6361 ctacattgag cgagttcggt cagaacagga tcgtcgtgtg gtgcatctgc atttgacaaa
     6421 gaagggtcgc ttgattcata ggctgcataa acgcttccac aaggccatgg tagaaaaaat
     6481 tattgatggc atgagcgagg aagaaattgc tgtcatgggt aaaggtttga ctaatcttta
     6541 ccaatttttg gaggatttga aataatggct tttgcaaaaa taagtcaggt tgctcattat
     6601 gtgccagagc aagtggttac aaatcacgac ttggctcaga ttatggatac caatgatgag
     6661 tggatttcaa gtcgaacggg aatacgacaa aggcatattt caagaacaga atctaccagt
     6721 gatttggcta cagaggttgc taagaaactg atggcaaaag ctggaataac aggaaaagaa
     6781 ctggatttta tcatcctagc taccattact ccagattcga tgatgccctc tacagctgct
     6841 cgtgttcaag ctaatattgg cgctaataaa gcctttgctt ttgacttaac cgcggcttgc
     6901 agtggatttg tatttgctct ttcaactgct gaaaagttta tcgcttctgg tcgctttcaa
     6961 aaaggcttgg tgattggtag tgaaaccctc tctaaggcag tcgattggtc ggatcgatca
     7021 acagctgtgt tgtttggaga tggtgctggt ggtgtcttgt tagaagctag cgagcaagag
     7081 catttcttag ctgagagtct caatagcgat ggaagtcgca gcgagtgttt aacttatggg
     7141 cattcaggtt tgcattctcc attttcagat caagaaagtg cagattcgtt tttgaagatg
     7201 gatggacgca cagtctttga ttttgccatt cgagatgtag ccaagtctat caagcagact
     7261 attgatgaat ctcctataga ggtgacagac ttggattatc tgctacttca tcaagccaat
     7321 gaccgtattt tggataagat ggctagaaaa attggtgttg accgagccaa acttccagcc
     7381 aatatgatgg aatatggcaa taccagtgca gccagtatcc cgattttact ttcagagtgt
     7441 gtagaacaag gtctcatccc tttagatggt agccagactg ttcttctatc aggcttcggt
     7501 ggaggcttga cctggggcac gctcattctt acaatttagg taatcatgtg gtgaacacat
     7561 tgttataaaa aaactattta ttttaaagga gtcctatcat ggcagtattt gaaaaagtac
     7621 aagaaattat cgttgaagaa cttggaaaag acgcatcaga agtaacactt gaatcaactt
     7681 ttgatgattt ggacgcagat tcattggact tgttccaagt aatctcagaa atcgaagatg
     7741 cttttgatat ccaaatcgaa gcagaaaatg acttgaaaac agttggtgac ttggttgctt
     7801 acgttgaaga gcaagcaaaa taagcagaat attagtagaa ggagtaggga gaaacccact
     7861 ccctcttgtt taggtaatag tttgactgtc aaattatggt gaaatcgaac tattacgtaa
     7921 gcaaggaagg ttggaggcac tatgaaaacg cgtattacag aattattgaa gattgactat
     7981 cctattttcc aaggagggat ggcctgggtt gctgatggtg atttggcagg ggctgtttcc
     8041 aaggctggag gattaggaat tatcggtggg ggaaatgccc cgaaagaagt tgtcaaggcc
     8101 aatattgata aaatcaaatc attgactgat aaaccctttg gggtcaacat catgctctta
     8161 tctccctttg tggaagatat cgtggatctc gttattgaag aaggtgttaa agttgtcaca
     8221 acaggagcag gaaatccaag caagtatatg gaacgtttcc atgaagctgg gataatcgtt
     8281 attcctgttg ttcctagtgt cgctttagct aaacgcatgg aaaaaatcgg tgcagacgct
     8341 gttattgcag aaggaatgga agctgggggg catatcggta aattaacaac catgaccttg
     8401 gtgcgacagg tagccacagc tatatctatt cctgttattg ctgcaggagg aattgcggat
     8461 ggtgaaggtg ctgcggctgg ctttatgcta ggtgcagagg ctgtacaggt ggggacacgg
     8521 tttgtagttg caaaagagtc gaatgcccat ccaaactaca aggagaaaat tttaaaagca
     8581 agggatattg atactacgat ttcagctcag cactttggtc atgctgttcg tgctattaaa
     8641 aatcagttga ctagagattt tgaactggct gaaaaagatg cctttaagca ggaagatcct
     8701 gatttagaaa tctttgaaca aatgggagca ggtgccctag ccaaagcagt tgttcacggt
     8761 gatgtggatg gtggctctgt catggcaggt caaatcgcag ggcttgtttc taaagaagaa
     8821 acagctgaag aaatcctaaa agatttgtat tacggagccg ctaagaaaat tcaagaagaa
     8881 gcctctcgct gggcaggagt tgtaagaaat gactaaaaca gcctttttat ttgctggtca
     8941 aggtgcccag tatctaggga tgggacggga tttctatgat cagtatccga ttgttaaaga
     9001 aacgattgat cgagcgagtc aggtgctagg ttatgatttg cgttatctca tcgatacgga
     9061 agaagacaaa ctcaatcaga cccgctatac gcaaccagcc attctagcga cttcggttgc
     9121 tatctaccgt ttattgcaag aaaagggcta tcagcctgat atggttgctg gtttgtctct
     9181 tggagaatac tctgccttgg tggcaagcgg cgccttggat tttgaagatg cggttgcctt
     9241 ggtagctaag cgtggagcct atatggaaga agcggctcct gctgactctg gcaagatggt
     9301 agcagttctc aatacgccag tagaggtcat tgaagaagcc tgtcaaaaag cttctgaact
     9361 tggagtggtt actccagcca actataacac acctgcacaa atcgtcattg ctggagaagt
     9421 ggttgcagtt gatcgagcgg ttgaactttt gcaagaagca ggtgccaaac gcttgattcc
     9481 tcttaaggtg tcaggtccct ttcacaccgc tctccttgag ccagctagcc agaaactagc
     9541 tgaaactcta gctcaggtaa gtttttcaga ttttacttgt cccctagtcg gcaatacaga
     9601 agctgctgtg atgcaaaaag aggacattgc tcagctcttg acgcgtcagg tcaaggaacc
     9661 cgttcgtttc tatgaaagta ttggggtcat gcaagaagca ggcataagca actttatcga
     9721 gattggaccg gggaaagtct tgtcaggttt tgttaaaaaa attgatcaaa ctgctcactt
     9781 agctcatgtg gaagatcaag cgagtttagt agcactttta gaaaaataga ctaaaataag
     9841 tagaagtttt gaaaggaaaa aaatgaaact agaacataaa aatatcttta ttacaggttc
     9901 gagtcgtgga attggtcttg ccatcgccca caagtttgct caagcaggag ccaacattgt
     9961 cttaaacagt cgtggggcaa tctcagaaga attgctcgct gagttttcaa actatggtat
    10021 caaggtggtt cccatttcag gagatgtatc agattttgca gacgctaagc gtatgattga
    10081 tcaagctatt gcagaactgg gttcagtaga tgttttggtc aacaatgcag ggattaccca
    10141 agatactctt atgctcaaga tgacagaagc agattttgaa aaagtgctca aggtcaatct
    10201 gactggtgcc tttaatatga cacaatcagt cttgaaaccg atgatgaaag ccagagaagg
    10261 tgctatcatt aatatgtcta gtgttgttgg tttgatgggg aatattggtc aagctaacta
    10321 tgctgcttct aaggctggct tgattggctt taccaagtct gtggcacgcg aggtcgctag
    10381 tcggaatata cgagtcaatg tgattgctcc aggaatgatt gagtctgata tgacagctat
    10441 cttatcagat aagattaagg aagctacact agctcagatt ccgatgaaag aatttgggca
    10501 ggcagagcag gttgcagatt tgacagtatt tttagcaggc caagattatc taactggtca
    10561 agtgattgcc attgatggtg gcttaagtat gtagcgaaag ctagaggtga aaagaatgaa
    10621 actaaatcga gtagtggtaa caggttatgg agtaacatct ccaatcggaa atacaccaga
    10681 agaattttgg aatagtttag caactgggaa aatcggcatt ggtggcatta caaaatttga
    10741 tcatagtgac tttgatgtgc ataatgcggc agaaatccaa gattttccgt tcgataaata
    10801 ctttgtaaaa aaagatacca accgttttga taactattct ttatatgcct tgtatgcagc
    10861 ccaagaggct gtaaatcatg ccaatcttga tgtagaggct cttaataggg atcgttttgg
    10921 tgttatcgtt gcatctggta ttggtggaat caaggaaatt gaagatcagg tacttcgcct
    10981 tcatgaaaaa ggacccaaac gtgtcaaacc aatgactctt ccaaaagctt taccaaatat
    11041 ggcttctggg aatgtagcca tgcgttttgg tgcaaacggt gtttgtaaat ctatcaatac
    11101 tgcctgctct tcatcaaatg atgcgattgg ggatgccttc cgctccatta agtttggttt
    11161 ccaagatgtg atgttggtgg gaggaacaga agcttctatc acaccttttg ccatcgctgg
    11221 tttccaagcc ttaacagctc tctctactac agaggatcca actcgtgctt cgatcccatt
    11281 tgataaggat cgcaatgggt ttgttatggg tgaaggttca gggatgttgg ttctagaaag
    11341 ccttgaacac gctgaaaaac gtggagctac tatcctggct gaagtggttg gttacggaaa
    11401 tacttgtgat gcctaccaca tgacttctcc acatccagaa ggtcagggag ctatcaaggc
    11461 catcaaacta gccttggaag aagctgagat ttctccagag caagtagcct atgtcaatgc
    11521 tcacggaacg tcaactcctg ccaatgaaaa aggagaaagt ggagctatcg tagctgttct
    11581 tggtaaggaa gtacctgtat catcaaccaa gtcttttaca ggacatttac tgggggctgc
    11641 gggtgcagta gaagctatcg tcaccatcga agctatgcgt cataactttg taccaatgac
    11701 agctgggaca agtgaagtat cagattatat cgaagctaat gtcgtttatg gacaaggctt
    11761 ggagaaagaa attccatacg ctatttcaaa tacttttggt tttggaggcc acaatgcagt
    11821 tcttgctttc aaacgttggg agaatagata agtatgaatt taaacgatat taaagacttg
    11881 atgactcaat ttgaccagtc aagtttgaga gaattttctt ataaaaatgg gacggatgag
    11941 ttgcagttta gcaagaatga agcaagacct gtgcctgaag ttgcaactca agtcgctcca
    12001 gcacccgttc tagcaacacc gagtccagta gctcctacat ctgctccagc agagactgta
    12061 gcagaagaag ttccagctcc agctgaagca agtgtggcta gtgagggaaa tcttgtagag
    12121 agtccacttg ttggagtggt ttacttggct gctggaccag ataaacctgc cttcgttaca
    12181 gttggtgata gtgtcaaaaa aggtcaaaca ttggtaatta tcgaagccat gaaagtcatg
    12241 aatgaaatcc cagctcctaa ggatggtgtg gtaacggaaa ttctcgtctc taacgaagaa
    12301 atggttgagt ttggtaaagg attggtacgt atcaaatgat cgatattcaa ggaatcaaag
    12361 aagctcttcc ccaccgttat cctatgcttc tagtggaccg tgtcttggaa gtgagcgagg
    12421 ataccattgt tgctatcaaa aatgtgacca ttaacgaacc tttctttaac ggccactttc
    12481 ctcaataccc agttatgcca ggtgttctga ttatggaagc cttggcgcaa actgctggtg
    12541 tgttggagtt atcaaaacct gaaaataaag gaaaactggt cttttacgct ggtatggaca
    12601 aggttaagtt caagaagcaa gttgtaccag gcgaccaatt ggttatgaca gcgacttttg
    12661 taaaacgtcg tggcaccata gctgtggttg aagcaaaggc tgaagtggat ggcaagcttg
    12721 cagccagtgg tatccttact tttgcaattg ggaactaagg aggttctcca tgtttcgaaa
    12781 aattttaatt gccaatcgtg gtgaaattgc ggttcgtatt atccgtgcgg cacgtgaatt
    12841 ggggattgcg acggtagcgg tttattcaac tgctgataag gaagctcttc atacgctttt
    12901 ggcagatgaa gcagtttgta ttggtcctgg caaggcaaca gagtcttatc tcaatattaa
    12961 tgcagttcta tcagctgcag tcttgactga ggcagaagct attcaccctg gttttggatt
    13021 tctcagtgaa aattccaaat ttgcgaccat gtgtgaagaa gtaggtatca agtttatcgg
    13081 tccatctggt catgttatgg atatgatggg ggataagatc aatgcgcgtg ctcagatgat
    13141 taaagcaggt gtgcctgtta taccaggttc agatggagaa gtgcataact ctgaagaagc
    13201 tttgattgtt gctgaaaaaa ttggctatcc tgttatgctc aaggcttcag caggtggagg
    13261 tggtaaaggg attcgtaagg ttgaaaaacc agatgacctc gtttctgcct ttgaaactgc
    13321 ctctagtgag gccaaggcca attatggcaa tggtgccatg tacatagaac gggttatcta
    13381 tccagctcgg cacattgagg ttcaaatcct aggtgatgag catggacatg tgattcactt
    13441 gggtgaacgg gattgttctc ttcaaaggaa taaccaaaag gttttggaag aaagtccctc
    13501 gattgcaatc ggaaaaacgc tgcgtcatga aataggtgct gctgctgttc gagcggcaga
    13561 gtttgttggc tatgagaatg caggaaccat tgaatttctt cttgatgaag caagtagcaa
    13621 tttctatttc atggagatga atactcgtgt tcaggtagaa catccagtaa cagagtttgt
    13681 ttcaggtgtt gatatcgtta aggaacagat ttgcattgcg gcaggtcagc ctttgtctgt
    13741 taagcaagaa gatattgtcc tacgtggtca tgccatcgag tgtcgtatca atgcagaaaa
    13801 cccagccttt aactttgctc caagtccagg taagattact aatctctatc tgccaagtgg
    13861 tggagttggc ttgcgcgtgg attcagcagt ttatccaggt tataccattc cgccttatta
    13921 tgatagtatg attgccaaaa tcatagtaca cggcgaaaat cgttttgacg ccttgatgaa
    13981 aatgcaacgt gccctctatg aattagagat tgaaggagtg cagaccaatg cagatttcca
    14041 gcttgacctc atttcagatc gcaatgtcat tgctggggat tatgatactt ccttcttgat
    14101 ggaaaccttc ttacctaaat atcaagaaaa agaataaaat gactttaaga gtttaaaccg
    14161 aaaaggggaa tcaatggctc tatttagtaa aaaagataag tatattcgaa tcaatcccaa
    14221 tcgttcggtt agggaaaaac ctcaagctaa gccagaggtt ccagatgaat tattttccca
    14281 gtgtccaggc tgtaagcata ccatctatca gaaggatctg ggaagtgaac gtatctgtcc
    14341 gcactgtagc tatacctttc gtatttctgc ccaagaacgc ttggctttga cgattgatat
    14401 gggaaccttc aaagaattgt ttacagggat tgaaagcaag gatcccttgc atttccctgg
    14461 ttaccaaaag aaactggcat ctatgcgtga aaaaacaggt ctgcatgaag ccgttgtgac
    14521 aggaactgct cttattaaag gtcagactgt ggctcttggg attatggatt ctaactttat
    14581 catggcttct atgggtacgg ttgtaggtga aaaaatcact cgtttgtttg agtatgcgac
    14641 tgtcgaaaaa ttgccagttg tcctattcac agcctctggt ggagcccgta tgcaggaagg
    14701 aatcatgagt ctcatgcaga tggctaagat ctctgcggcg gttaaacgcc attcaaatgc
    14761 tggtctcttt tacctgacca ttttgacaga tccaacgact ggtggtgtga cagcttcttt
    14821 cgctatggaa ggcgatatca ttctggctga accacagagc ttggttggtt ttgctgggcg
    14881 tcgtgtgatt gaaaatacgg ttcgtgaaag cttgcctgag gatttccaaa aggcagaatt
    14941 cctattagaa catggctttg tggatgctat tgtcaaaaga agagacttac cagatacgat
    15001 tgctagccta gtcagattgc atggagggag tcctagatga atattgcaaa aatagtcaga
    15061 gaagcgcgtg agcagagtcg cttgacaacc ttggactttg cgacaggcat ttttgatgaa
    15121 tttatccaat tacatggtga ccgttctttt cgtgatgatg gtgcagttgt tggtggtatt
    15181 ggttggcttg gagaccaagc tgtaacagtt gttggtatcc aaaaaggcaa gagtttgcaa
    15241 gacaacctca aacggaattt tggccaacca catccagaag gctaccgaaa ggcactgcgg
    15301 ttgatgaaac aggctgagaa atttggccgt ccagttgtga cctttattaa tacagcaggt
    15361 gcttatcctg gtgtcggagc agaagaacgt ggtcaagggg aagctatcgc tcgcaatctc
    15421 atggaaatga gtgacctgaa agttcctatt atcgccatta ttatcggtga aggtggttca
    15481 ggcggggctc tggctctagc tgtcgcggac cgtgtctgga tgctggaaaa ttctatctat
    15541 gccattctca gtccagaagg ctttgcttcc attttatgga aggacggtac tcgcgccatg
    15601 gaagcagcag aactgatgaa aatcacttcg catgaactgt tagaaatgga cgtggtggat
    15661 aaggtgattt ctgaagtagg actttctagt aaagaactga ttaagagtgt caaaaaagaa
    15721 ctccaaacgg agctggctag actttcacaa aaaccgctag aagagttgct ggaagaacgc
    15781 tatcaacgat ttagaaaata ctaagaatga ctaaatgaga ctagaaaggt ctcatttttt
    15841 atggaaaaat gcaattcagg ttgaaaaatt gacagttgag gagatagact ataaaataga
    15901 caaaaaaaga aatatactat aaatgtgagc ctacaacaat ctattttaag aaggtacttg
    15961 caatgacagg tacaaataca tttacagttc tttcaactga ggacttggag caaacttcag
    16021 gtggtcttgc tgtttgggaa gatggatata gtagatggtt atattataga gaatttgctc
    16081 cctatatgag gcaaggggca cttaattctt atatagatgc ttggaagtac ggcttccgag
    16141 cagggtaatc tttcaaaaaa gtacgatgag aaacacaaaa aaactaagac aatttggaat
    16201 atttttgctt attattttac tatctaccta tttaccacaa acaattggat tgtatgtgac
    16261 gatcatttta gggttaggag ctgatgtcta ttctcttatt ttaacaatgg tattagtggg
    16321 aagtttcctt ctattgattt ggaggttaaa aaagaaaaag atgctcttta tctttgaaaa
    16381 aaagagctgg aactggtcat ttgtcttcta cttatttgca acgtatgtgg tttatcagat
    16441 tctcggtaat ttttaggcac gctatgctca tttgattaac cataggaata ttcatgatga
    16501 gtattttacc gtggttcttt caaatggaca accaaccttt ttatcgacta tcttatcttt
    16561 tgtcctgctt gtaatcatcg gtcctgtttt tgaggagact cttgatagag ggtatttgat
    16621 gaataccttc tttcctaagt caaagtacta tttagatgtc atcttatcag gccttatctt
    16681 tgggattagc catttgatac tatctcacag agatccaatt agtttattat attatagtct
    16741 gattggtttc ttttttgccc ttgtttatcg ttctaccgac aatctgagat taacgattct
    16801 ctgccatagt ttttttaact ttctcaatca tgcaaaacct atctggattt ttgtttacaa
    16861 ttatatctat tatcattttt ttagatagtg ggaggcaaag aaaaagtgtt tgatatctat
    16921 caaacacttt ttctttttat tgttcttctg ttacaaactg gctgagcagt ccattgataa
    16981 aacgggcaga tttttgatcg gagaagtcct ttgcaagctc gatagcttca ttaacagcaa
    17041 ccagctgagg agtgtcaaat gaagtgattt caaaaactcc caagcgaagg aggtttctct
    17101 ccacgagcgt taagcgttca atggtccaac ctacttttaa atgctgagtg atttgcttat
    17161 ctagttcttc ctttttagct tgaacaccag aaacgaggtc tatcaaaaag gctggaagtt
    17221 gtacatccgt atcttcacga tcatgagtat aggcgaaacg acaagcagtt tcgacattcg
    17281 taccgaactc aaggctcatg agagcttgaa aagcgcattt acggagttgg cgtctagatt
    17341 ctaatagtgg actagtcatt gaggaagtcc tcgtcaaata gatctttcaa ttctggtttt
    17401 ggtgttttat ctgggacgat acctgcaacg tgaatattga tagcagcgag ttctacatca
    17461 gccatattac ggacggcatc tttgacagct ttctggatag caaccgctac cttaggaact
    17521 tttactccgt actcaaggta gagatagata tctgctgtga gttcttcgtc cacgttttta
    17581 agataaacgc cacggccgag tgaaagtttt gaaagggtat cagacactga tctgtttgaa
    17641 aaagagtgaa caccctctac ctttgcagta gcgatagcaa tgattttttc aagtacacgt
    17701 ggggcgataa cgatttcgcc aagttgttct tcaattccca tagaatgacc tctttctaga
    17761 gattaggcac gagaaacgta agttccttct gcagtgttga taacgagttt ttgtcctgct
    17821 tcgatgaagt ctggaacgtt tacgacaagt ccagtttcca tcgttgctgg tttgccagaa
    17881 cctgtaacag tagcaccttt gatagatggt tgagtttcag caactgtcaa ctcaacagta
    17941 gtaggaacgg tgacaccgat cacttcagtt ccgtagaatt ggattttcac atcagagttt
    18001 tcaaggatat aaagcaattc gttttcaaca ttgactacag ggatttcgta ctggtcgtaa
    18061 gtttctgtat tcatgaagta tgctgtgtca tccattttgt acaagtattg agctgggaca
    18121 gtctcgataa tagcttgttc aaatttttcc tctggacggt agcttgtgtc aaatgtagaa
    18181 ccagtacgga catcacgcaa tttcatacgc atgatcgtgt ttcctttacc tggcttgtgg
    18241 tgactagctt ccaaaacgcg aatcaatttg ccgtcagctg tttcaaaggt cataccagct
    18301 tttaatttac ttgcttcaat catgttttta cctctttaat aaatattatt actcttcatt
    18361 ttaccataaa atactctgga aataaagcca aaatagttat tgggagttgg tagagaagaa
    18421 aaaaccaacc ttagggctgg ttttgttctg tctagttttc tttcaacttc gccaattcct
    18481 gtgcaagtag tttaagggca acttgtgggt tggcttggcc tttggttgcc ttcataagga
    18541 atcctgtaaa ggccttgtcg gcgttacgtt tgcctgactt gaagtcggca acagcagctt
    18601 cgttatcggc aaagacttgg tggatgattg ggatcaagat agctggatct gaaatttgaa
    18661 ccatacctgc tttttccacg tattcacgcg cgccaccgcc atttttagct agatggacaa
    18721 agactttctt ggcaatctta gatgaaatag taccgtcttc gatgatggta atcatttcaa
    18781 ccaagttttc tggtgtcaat tcgatttgtt ccagtgtttt accttcagca ttcaagaact
    18841 gagcgacttc cccttggagc cagttagaga cttgtttggc atcaccacct agggcaacag
    18901 ctttttcaaa gaagtcagaa gtgactttat tagcagtcaa ctgactagca tcgtagtctg
    18961 ataaaccaag gtcagataca taacgcgcac gacgttcttt tggaaactct ggcaactcag
    19021 tccgcatttc ctcaatccac tcgtcagaaa tttcaaagag gggtaggtct ggttctggga
    19081 agtagcggta gtcagcagcc ccttccttga cacgcatgag gatggttgtt ttgttagctt
    19141 catcgtaacg gcgtgtttct tggcggattt gaccacctga gcgaagaatt tcagcctggc
    19201 gttggacttc gtattcaaga cctttacgaa cgtttgagaa ggagttgagg ttcttcaatt
    19261 cagtcttggt accgaatttt tcttgaccat aagggcgaag ggagatgttg gcatccacac
    19321 gcatagaacc ttcttccatc ttaacgtcag aaatgccagc gtactggata acttccttga
    19381 gggctgtcag ataagcatag gcctcctctg gcgaacgcat atcggcttca gatacaatct
    19441 caatcaaagg taccccttgg cggttaaggt caacgtaaga gtagccgtct gtaccgtggg
    19501 tgtttttacc agcgtcttcc tctaagtggg cgcgttcgat accaattttc ttggtcgtac
    19561 cgtcttctag ctcgacttca atccagccgt tataaccgat tggctcatca aactgagaaa
    19621 tttggtaggc tttgggatta tcagggtaga agtagttctt gcggtcaaag tgcatttttt
    19681 tgtggaggtc catgttgagg gcaagagcag ccttgatacc ggcatcaaca acccctttat
    19741 tgagaactgg tagaactcct gggaaagacc agtcaatcac gttagtgttg gcattttggt
    19801 catttccaaa gtgggcagaa gtaggtgaga agatttttga attggtgttg agctctacgt
    19861 ggacttcaag tccgatgact gtttcaaagt tcattagttg tcacctccaa aaatcacggg
    19921 ttgttgtttg tggtagtctg ttgttgcttc aaaagcagca gcagcttggt aaatggtttc
    19981 ctcagagtac ttgggaccaa tcaattggag tccgacaggt agaccttgag agaatccagc
    20041 aggaatcgaa attccaggca gtcctgccaa gtttacaggt atggtcaaga ggtcggctaa
    20101 gtacatggca actgggtcat ggttgagaga atccaagtca taggcaacac ttggagcagt
    20161 tggacccaaa atcaaatcgt aatccgcgaa gactttttcg aaatcttgaa tgataagtgt
    20221 acggacctgt ccagccttct tgtagtaggc atcgtagtaa cctgatgaaa gactgaaagt
    20281 acccagcatg atacggcgct tcacttcttc accaaaacct tggctacggc tgtttacata
    20341 gatttcatca agattagtcg catcttctgc gcggtagccg taacggatac cgtcaaaacg
    20401 ttgcaagttt gatgaagctt ctgatgaagc gatgatgtaa taaacggcaa caccgtattt
    20461 agagtgagga aggctgactt cttcgacgat agcacccaat ttttcaaagt gtttggccgc
    20521 gtttaagatt gtttccttaa cctctggatc aattccttcg cctaggtatt ccttaggcaa
    20581 agcaattttc atgcccttaa tgtcttggcc gatttttgaa gtaaagtcgg cgatgcggac
    20641 aggagcagaa gtagagtctt tagtatcttc gctggcaata gcgttgagca agagggcatt
    20701 ttccttaaca gtaggagcaa aaggtccaat ctggtctaat gagctaccaa aggcaatgag
    20761 accgaaacgt gaaactgttc cgtaggttgg tttgagacca acgattccgt tgaaggcagc
    20821 aggttggcgg atggaaccac cagtatcaga accaagtgac aagcgaactt gtcctgaggc
    20881 tacagctgcg gcagaaccac ttgatgaccc accaggaacc ttgctgtggt cccaagcgtt
    20941 tttagttgct ccgtagtgtg aagtttcacc ggaaccaccc atagcaaatt cgtccatgtt
    21001 ggtctttcca acgacaatca tgcctttggt ttttgcattg gcaacagctg tcgcatcaaa
    21061 gattggctca tagttgtaga gcatttttga ggcagcagtt gtgagaatac cgtctgtaga
    21121 gatgttatcc ttaacagcaa gtggaattcc tgaaaggaca ttgttagcat caattccagc
    21181 ttcatcaatg gctttagctt gaacaagagc ttgctcctca gcgatggtga caaatgaatt
    21241 gagggcttcc tcacgagact tgatattttc aagtgttgct tgggtcaatt ctgttgcaga
    21301 aatttcctta gagacaagga gattgtgcaa ctcttcaata gttttattgt taaaagtcat
    21361 taggcatctc ctccatcgtc taggatagct ggcaccttga tatagtagtt gtcttgttca
    21421 ggtacgtttt taaacaagcg atcacggtct gttccttctt cggccacatc agggcggagt
    21481 acagtcttgc ggtcagccat agtcgtagta ggtgcgacac cagttgtgtc aacttcgccc
    21541 agcaattcaa ccatgtcaac aatcttagac aaggtggtcg caaaggcagc agtttcttct
    21601 tcagagaatc ttaattttga aagattggca acgtgtgtta cctcttcttg cgtaattttc
    21661 atcttgcatc ctttcgtgaa atgatgattt ttagtctgtt ctattttacc atattttcct
    21721 ataaataagg gagggggaag tgaaaagaaa tagaaattga aaaaaatgct aaaatccgat
    21781 ataatggaat gttgaaagga atggtaaaat ccaatgtaaa aatcattctc agctattgaa
    21841 acaagaaaaa tagagaatca aagaaagaga acttatgaat attcaagaag aaattaagaa
    21901 acgtcgtacc tttgccatta tctcccaccc ggacgcgggg aaaacaacca tcactgagca
    21961 gttactctac tttgggggtg agattcgtga ggctggtacg gtaaaaggga agaaaacagg
    22021 gacttttgct aaatctgact ggatggatat cgagaagcaa cgtgggattt ctgttacttc
    22081 atctgttatg caatttgact acgacggcaa gcgcgtgaat atcttagaca cgccagggca
    22141 cgaggacttc tcagaagata cctatcgtac cttgatggcg gtggatgctg cggtcatggt
    22201 cgtggactct gccaagggga tcgaggctca aaccaagaaa ttgtttgagg ttgtgaaaca
    22261 tcgtggcatt ccagttttta cctttatgaa caagctggac cgtgacggtc gtgagccttt
    22321 ggatctcttg caagaattgg aagaaatctt gggcattgct agctacccta tgaactggcc
    22381 tatcgggatg gggaaagcct ttgagggctt gtatgacctc tataaccaac gtttagagct
    22441 ttacaaaggg gatgagcgtt ttgccagtct agaagacggg gacaaactct ttggtagcaa
    22501 tcctttctac gagcaagtca aggatgacat tgagctttta aatgaagctg ggaatgagtt
    22561 ttcagaggaa gctattctgg ctggagaatt gacgcctgtc tttttcggtt cagccctgac
    22621 aaactttggt gtgcagacct tccttgaaat cttcctcaag tttgctccag aaccacatgg
    22681 tcacaagaaa acagacggtg aaattgtgga tccttatgac aaggatttct caggctttgt
    22741 ctttaaaatc caagccaaca tggatcctcg tcaccgtgac cgtattgcct ttgtccgtat
    22801 cgtatcaggc gaatttgagc gtggcatgag tgtcaatctc cctcgtactg gtaagggtgc
    22861 caaactatct aatgttaccc agtttatggc ggagagtcgt gagaatgtga ccaatgccgt
    22921 atcaggtgat attatcgggg tttacgatac cggtacttat caggttgggg ataccttgac
    22981 ggttggaaaa aacaagtttg aatttgaacc actgccaacc tttactcctg aaattttcat
    23041 gaaagtttct gctaagaatg ttatgaagca aaaatccttc cacaagggga ttgagcaatt
    23101 ggtgcaagaa ggagccgttc agctttataa gaattaccaa acaggtgagt acatgctggg
    23161 agctgttggt caactccagt ttgaagtctt taaacaccgt atggaaggcg aatacaatgc
    23221 ggaagtggtc atgagcccaa tgggtaaaaa gaccgttcgt tggatcaagc ctgaggactt
    23281 ggatgaacgg atgtcgtcaa gtcgcaatat cttagccaaa gaccgttttg accaaccagt
    23341 ctttctcttt gaaaatgact ttgccctccg ctggtttgcg gacaagtatc cagacgtaga
    23401 gttagaggag aagatgtgac tcagtaccaa caattggaac taaagttcca aattgttgga
    23461 cgctagtcgc tatttggcga actagctaac tgccttacta actacgtttg gcaaatgaaa
    23521 tcgatttgta aaactccgtg tcgtaatgta aaaattgtaa accgtaatgg gacttcatct
    23581 aaaatctcca ctggtatggt tttacgaacg aatgggcgaa aataatggtt acgtagaaac
    23641 atatgatggg aataaaaaga aatgagataa agagtgaaag aagagaaaaa agcgattgtc
    23701 ttaggtgcag ataatgctta tatggataaa gttgaaacaa caataaaatc tctttgtgtt
    23761 catctattat aatcttaagt tttatgtttt taatgatgac cttccgcgag aatggttcca
    23821 attgatggaa aaaagactag agactttgaa tagtgagatt gtgaatgttt agattgacag
    23881 tagtattctg aaaggttatc gtctcccctt tgagggactt tcatatgcgg cttttttagg
    23941 tattttattc caaagtatgt aagcgagtct cgtgtactgt atttggattt agatattgtt
    24001 gtaagaaaat cgatagatga gctgtgggat ttggacttga cggctatacc cttagctgca
    24061 gttagagatg atttttatac acataatttt aattctggtg ttctgttaat caacaatggc
    24121 atgtggagag cagaaaatgt cacgcaagac ctgatttaag acacaaataa aaaatcttcc
    24181 aattcccttg caaaaaacaa gaaagtgtga taacatagta cggtatgtgg tgctagcaca
    24241 tccgctatat tagatctaat aggaggaaaa cacaatggct aaagtatgtt actttacagg
    24301 tcgtaagact gtatcaggaa acaaccgttc acacgcgatg aaccaaacaa aacgtgccgt
    24361 aaaaccaaac cttcaaaaag ttactgttct tatcgatggt aaacctaaaa aagtttgggc
    24421 ttcagctcgt gctttgaaat caggtaaagt tgaacgcgtt taataataaa agcaaaggag
    24481 accttagggt cttttttctt ttattgtggg tataacatcg tttgaaaaat agagtaaata
    24541 tccgctgata catcattctg cttttacact tgggctgaaa tatgataaaa tagagtatca
    24601 actagttgag gtaaaaagga tgactgtaaa aattaataca aaagatggtc aaatcgaact
    24661 aacagatgaa gtgattgcaa ccgtcgtagg tggagcagca actgagattt ttggtgtggt
    24721 cggtatggct agtaaaaatg ccctcaaaga taatttccaa gccctgctag gtaaggaaaa
    24781 ttattctaaa ggtgtcgtcg taaaggcagc cgaagatggc agtattgcag ttgatgtata
    24841 taccgtgtta agctacggaa caaagattag tgaagtgtca aaaaacattc aagagcgtgt
    24901 tgcctttagc ttggaaaacc agcttggaat tactgctcag actgtaaatg tctacattca
    24961 aaatatcaaa gttgtaggag aataaccgtg tcaaaaatta ctactagctt atttcaagaa
    25021 atggtgcagg ctgcatcaac tcgcttgaat aagcaagctg aatatgtcaa ttcattaaac
    25081 gtctttccag ttccagatgg agatactggg acaaatatgg gaatgaccat tgaaaatggt
    25141 gctaaagaag ttgcagacaa gccagcttct acagttggag aagtagcgag cattcttgct
    25201 aaagggcttt tgatgggtgc gcgtgggaac tcaggagtga ttacgtctca gcttttccgt
    25261 ggattttccc aagctatcaa ggataaagac gagttaacag gtcaagactt ggctctggcc
    25321 ttccaatcag gtgtggaagt tgcctataag gcagtgatga aacctgttga aggaacgatt
    25381 ttgacagttt ctcgtggagc tgctatcggt gctaagaaaa aagctgagca aacagatgat
    25441 actgttgaag tcatgcgcgc agccttggaa ggtgctaaaa cagctctagc taaaacacca
    25501 gacatgcttc cagtattgaa agaagttggc gttgtggact caggtggtca aggattggtc
    25561 ttcatctacg aaggtttcct ttcagccctt actggcgaat atattgcatc tgaggacttt
    25621 gtagcgactc ctgccaacat gagtgagatg atcaatgtag agcatcataa gtctgtagct
    25681 ggtcacgtag cgactgagga catcacgttt ggttactgta ctgaaatcat ggtagctctt
    25741 aagcaaggtc caacctatgc taaagatttt gactacgatg aattccgtaa ctacttggat
    25801 gagcttgggg attctctcct cgttgttaac gatgatgaaa ttgtcaaagt ccatgttcat
    25861 acagaagatc caggacttgt tatgcaagaa ggtctcaaat atggtagctt ggtcaaggta
    25921 aaagttgaca atatgcgtaa ccaacacgaa gcacaggttg agaaagaagc tactcaagtt
    25981 atcaagtcgg ctgaagaaaa agagtatgct ttgattgctg tggtggctgg taaaggtcta
    26041 gcagatatct tctgttctca aggcgtggat tatgttatcg aaggcggtca aaccatgaac
    26101 ccttcaacag aagactttat caaggctgtt gaacaggtca atgcccgtaa catcatcttc
    26161 ttgccaaaca acaagaacat cttcatggca gctcaatctg cggcagaagt tttggagcaa
    26221 ccagcagtag tggtagaggc tcgcactctt cctcaaggta tgacaagtct tcttgccttt
    26281 gatccaagca agtccattga agaaaaccaa gagcgtatga cagctgctct tagagatgtc
    26341 gttagcggaa gcgtcacaac agccgtgcgt gatacaacga tcgatggctt agaaatccat
    26401 gaaaacgata atctaggtat ggtggatgga aaaattcttg tgtcaaaccc tgatatgcac
    26461 caaacattga ctgaaacctt gaaacatatg ttggatgaag acagtgaaat cgtaaccttc
    26521 tatgtcggtg aagacggaag cgaagaactt gccaatgaaa tcgctcaaga aatcgtagaa
    26581 gaattcgaag acgttgaagt cgagattcac caaggtcaac aacctgttta cccataccta
    26641 tttagtgtgg aataagatta atgaaggact gagaaatcag ttcttttttc ttttttagta
    26701 aatgaaatcg gtatcttttt aataaaaac
//
LOCUS       DAVLYV010000017        25134 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLYV010000017 DAVLYV010000000
VERSION     DAVLYV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.017"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            127..624
                     /locus_tag="VYO70_000791"
     CDS             127..624
                     /locus_tag="VYO70_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1713058.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            624..2279
                     /gene="dnaX"
                     /locus_tag="VYO70_000792"
     CDS             624..2279
                     /gene="dnaX"
                     /locus_tag="VYO70_000792"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1713059.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYTPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKSEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQIEKEVEESLIPEGFEFLADKVKVEED"
     gene            2307..2501
                     /locus_tag="VYO70_000793"
     CDS             2307..2501
                     /locus_tag="VYO70_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1713060.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2651..3421
                     /gene="sufC"
                     /locus_tag="VYO70_000794"
     CDS             2651..3421
                     /gene="sufC"
                     /locus_tag="VYO70_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1713061.1"
                     /translation="MSVLEIKDLYVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3449..4711
                     /gene="sufD"
                     /locus_tag="VYO70_000795"
     CDS             3449..4711
                     /gene="sufD"
                     /locus_tag="VYO70_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1713062.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLLGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4722..5948
                     /locus_tag="VYO70_000796"
     CDS             4722..5948
                     /locus_tag="VYO70_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1713063.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINACSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5935..6375
                     /locus_tag="VYO70_000797"
     CDS             5935..6375
                     /locus_tag="VYO70_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1713064.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDVVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6429..7841
                     /gene="sufB"
                     /locus_tag="VYO70_000798"
     CDS             6429..7841
                     /gene="sufB"
                     /locus_tag="VYO70_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1713065.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8072..9313)
                     /locus_tag="VYO70_000799"
     CDS             complement(8072..9313)
                     /locus_tag="VYO70_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1713066.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9416..10525
                     /locus_tag="VYO70_000800"
     CDS             9416..10525
                     /locus_tag="VYO70_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1713067.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLIWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11171..11518
                     /gene="ssrA"
                     /locus_tag="VYO70_000801"
     tmRNA           11171..11518
                     /gene="ssrA"
                     /locus_tag="VYO70_000801"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11691..11778)
                     /locus_tag="VYO70_000802"
     tRNA            complement(11691..11778)
                     /locus_tag="VYO70_000802"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11742..11744),aa:Ser,seq:gga)
     gene            11941..12681
                     /locus_tag="VYO70_000803"
     CDS             11941..12681
                     /locus_tag="VYO70_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1713068.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12678..13589
                     /gene="pfkB"
                     /locus_tag="VYO70_000804"
     CDS             12678..13589
                     /gene="pfkB"
                     /locus_tag="VYO70_000804"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1713069.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSDGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            13586..15538
                     /locus_tag="VYO70_000805"
     CDS             13586..15538
                     /locus_tag="VYO70_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1713070.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15702..17927
                     /locus_tag="VYO70_000806"
     CDS             15702..17927
                     /locus_tag="VYO70_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1713071.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(17972..18400)
                     /locus_tag="VYO70_000807"
     CDS             complement(17972..18400)
                     /locus_tag="VYO70_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713072.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            18538..19680
                     /locus_tag="VYO70_000808"
     CDS             18538..19680
                     /locus_tag="VYO70_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1713073.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHSLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAQSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19689..20903
                     /gene="thiI"
                     /locus_tag="VYO70_000809"
     CDS             19689..20903
                     /gene="thiI"
                     /locus_tag="VYO70_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1713074.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYPQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSAVQEIMQDIYKE
                     GMTFKISSKRSDHTFELDSRELNQTLGGAVFEAIPNVQAQMKSPDINLQVEIREEAAY
                     LSYETVRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            21038..21862
                     /locus_tag="VYO70_000810"
     CDS             21038..21862
                     /locus_tag="VYO70_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1713075.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPEQQFGGKGVEYAEFVMEVVKPFIKHKTGWFDGMMTTGCSMGAYHALNFFLQHP
                     DVFTKVIALSGVYDARFFVGDYYNDDAIYQNSPVDYIWNQNDGWFIDRYRQAEIVLCT
                     GLGAWEQDGLPSFYKLKEAFDQKQIPAWFAEWGHDVAHDWEWWRKQMPYFLGNLYL"
     gene            21875..23041
                     /locus_tag="VYO70_000811"
     CDS             21875..23041
                     /locus_tag="VYO70_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1713076.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            23549..25042
                     /locus_tag="VYO70_000812"
     CDS             23549..25042
                     /locus_tag="VYO70_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1713077.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            25055..>25134
                     /locus_tag="VYO70_000813"
     CDS             25055..>25134
                     /locus_tag="VYO70_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1713078.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTL"
BASE COUNT         7477 a         4648 c         5523 g         7486 t
ORIGIN      
        1 taaaataggt tccataatat ctatagtgga tttacccact acaaatatta tagagcccaa
       61 aatcctttgt ttactaaaca agggattttt cttttgtctc tgctcctttt ttgatataat
      121 agttctatgt taaaatcaga aaaacaatca cgttatcaaa tgttaaatga agaattgtcc
      181 ttcctattgg aaggcgaaac caatgttttg gctaatcttt ccaacgccag tgctctcata
      241 aaatcacgtt ttcctaatac cgtatttgca ggcttttatt tgttcgatgg aaaggaattg
      301 gttttaggtc ccttccaagg aggtgtttcc tgcatccgta ttgcactagg caagggtgtt
      361 tgtggtgagg cagctcactt tcaggaaact gttattgttg gagatgtgac gacctatctc
      421 aactatattt cttgtgatag tctagctaaa agtgaaattg tggtgccgat gatgaagaat
      481 ggtcagttac ttggagttct ggatctggat tcttcagaga ttgaggatta cgatgctatg
      541 gatcgagatt atttggaaca atttgtcgct attttgcttg aaaagacaac atgggacttt
      601 acgatgtttg aggaaaaatc ttaatgtatc aagcacttta tcgaaaatat agaagtcaaa
      661 acttctccca gttagttggt caagaagttg tggctaagac tcttaaacaa gcggtggagc
      721 aagagaaaat aagtcacgct tatctttttt ctggtcctcg tggaacggga aaaaccagtg
      781 ttgctaaaat ctttgccaag gctatgaact gtcccaatca agtgggtggc gaaccttgca
      841 ataactgcta tatttgtcaa gcagtgacgg acggtagttt agaagatgtc attgaaatgg
      901 atgcagcttc taataatggg gtagatgaaa ttcgcgaaat tcgtgataaa tctacctata
      961 cgcctagcct tgctcgttat aaggtttata tcatagatga ggttcacatg ctgtctacag
     1021 gggcttttaa tgccctccta aagacgctgg aagaaccaac acagaatgta gtctttattt
     1081 tggccactac tgaattgcac aagattcctg ctactattct atcccgtgtg caacgttttg
     1141 agtttaaatc aattaagaca caggatatta aggaacatat tcactatatc ttagaaaaag
     1201 aaaatatcag ttctgaacca gaggctgtgg aaatcattgc cagacgggcg gaaggtggaa
     1261 tgcgggacgc cttgtctatt ttggatcaag ccctgagttt gacacaggga aatgagctga
     1321 cgactgctat ctctgaagaa attactggca ccattagcct atcagccttg gatgattatg
     1381 tggcggcctt gtctcaacag gatgttccca aagctttgtc ttgcttgaat cttctttttg
     1441 acaatggtaa gagcatgact cgttttgtga ccgatctttt gcactattta agagacttgt
     1501 taattgttca aacaggggga gaaaatactc atcatagttc agtctttgta gaaaatttgg
     1561 cacttcctca aaaaaatctg tttgaaatga ttcgcttagc aacagtgagt ttagcagata
     1621 ttaagtctag tttgcagccc aagatttatg ctgaaatgat gaccgtccgt ttggcggaaa
     1681 tcaagtccga accagcttta tcaggagcgg ttgaaaatga aattgctacg ctgagacagg
     1741 aagttgcccg tctcaaacaa gagctttcta atgtaggtgc ggttcctaaa caagttgcac
     1801 cagctcctag tcgaccagct acgggcaaaa cagtctatcg tgtcgatcgc aataaagtgc
     1861 aatctatctt acaagaggcc gtcgaaaatc ctgatttagc acgtcaaaat ttaattcgtt
     1921 tgcagaatgc ctggggagag gtaattgaaa gtctaggtgg gccggacaag gctctgctag
     1981 ttggttctca accggttgct gccaatgaac accatgctat tcttgctttt gagtctaact
     2041 tcaatgctgg tcaaactatg aaacgagaca atctcaatac catgtttggt aatatcctca
     2101 gtcaggcggc aggtttttca cctgagattt tagctatttc catggaggaa tggaaagaag
     2161 ttcgcgcagc cttttcagcc aaagccaaat cttctcaaat tgaaaaagaa gtagaagaaa
     2221 gcctgattcc agaaggattt gaatttttgg ctgataaagt gaaggtagag gaagactaaa
     2281 gaaagatttc atgatacaat aagtttatga ataaacaaca atttattatt atggcgctgt
     2341 ttacagctgc tgagacctat tttttcaatg aagcctggat gactggtcgc tatattatgg
     2401 cagccttttg ggcaatttta ctctttagaa atttccgagt cagttatgtg atgggcaaaa
     2461 tcgttgatgt catcgatcag cattttaata ggaaagacta gccctcagct tccagacaaa
     2521 atcaaagcct tttaggcttt tttttgttat actagaaaag tatatttata gaatttttgc
     2581 tctatttctg gggaaatcag acgtttttct agtaagtact gtaaaagttt tgaaaaagaa
     2641 aggaactatc atgtcagtat tagagatcaa agatctttac gttgagattg aaggaaaaga
     2701 aattttaaaa ggggttaacc tgaccctgaa aacaggagaa attgccgcta tcatgggacc
     2761 aaatggtaca ggtaaatcga ctctttctgc cgctatcatg ggaaatccaa actatgaagt
     2821 aactaaaggt gaagttttgt ttgatggcgt aaacatcctt gagttggaag tggatgagcg
     2881 tgcgcgtatg ggacttttcc ttgctatgca atacccatca gaaatccctg gaattaccaa
     2941 tgctgagttt cttcgtgccg ctatgaatgc gggtaaagaa gatgatgaga agatttcagt
     3001 tcgtgagttt attactaagc tagatgaaaa aatggaattg ctcaacatga aagaagaaat
     3061 ggcagagcgt tacctcaacg aaggcttctc tggtggtgag aaaaaacgca atgaaattct
     3121 tcaacttttg atgttggagc caacatttgc tcttttggac gagattgact caggtcttga
     3181 tattgacgct cttaaagttg tgtctaaagg tgtcaatgcc atgcgtggtg aaggttttgg
     3241 tgctatgatc atcactcact accaacgtct tttgaactat atcacacctg atgtggtaca
     3301 cgtgatgatg gaaggtcgtg ttgtcctttc tggtggtcca gaattggctg cgcgtttgga
     3361 acgtgaagga tacgcaaaac tagctgaaga acttggctac gactacaagg aagaattgta
     3421 attccctcgt atcttttagg agaagtaaat gactagagaa aatattaaac ttttttcaga
     3481 aatgcacgct gaaccaagct ggttggctga tctccgtcaa aaagcttttg acaagattga
     3541 gactttggaa ttaccagtta ttgagcgtgt caaattccac cgttggaatc tgggtgatgg
     3601 aacgattaca gaaaatgagc catcagcaaa tgttccagat ttcacagctt tagatcatca
     3661 cttgaagttg gtgcaagtag gaactcaaac tgttttcgaa caaactccag ttgagttagc
     3721 tgaacagggt gttgtcttca cagactttca ctcagcttta gaagaaattc cagagctgat
     3781 cgaagaattc ttcatgtcat ctgttaagta tgatgatgac aagttggcgg cttaccacac
     3841 agcttacttt aacagtggtg ctgtactcta tattccagat aacgtagaaa ttacagagcc
     3901 aattgaagga attttctacc aagatagcga tagcaatgtg ccgtttaaca agcatattat
     3961 gattatcgct ggtaaaaatt ctaagattag ttatctggag cgtttagagt cacgcggtga
     4021 aggaagtgac aaagcaactg ccaatatcac agtggaagtg attgcacgtt ctggtgcgca
     4081 agtcaagttt gctgctatcg accgtctagg tgaaaacgtc actgcctaca ttagccgtcg
     4141 tggtaaatta ggcaacgatg caagtattga ctgggctatc ggtgtcatga acgaaggaaa
     4201 tgtcgttgct gattttgata gtgacttgct tggaaatggt agccatgcgg acctcaaggt
     4261 agtagcactt tcaagtggtc gtcaggtgca agggattgat actcgcgtga ctaactatgg
     4321 ttgcaactct atcggaaata tcctacaaca tggggttatc cttgaaaaag caactttgac
     4381 tttcaatggt atcggccaca tcatcaaggg tgctaaggga gcagatgccc aacaagagag
     4441 ccgtgttctc atgctttcag accaagcgcg ttcagatgct aacccaattc ttttgattga
     4501 tgaaaatgat gtcactgcag gacacgcagc gtctatcgga caagttgatc cagaagacat
     4561 gtattacctc atgagtcgtg gattggataa ggcaactgca gagcgtttgg ttgttcgtgg
     4621 tttccttgga tctgttatcg tggagattcc agtcaaggaa gttcgtgatg aaatgattgc
     4681 aactatcgaa gagaaattgt caaaacgcta aggggaagcc tatgttagat gtagaagcga
     4741 ttcgcaagga ttttccaatt ttagatcaga ttgtcaatga tgaacctctg gtctatctgg
     4801 acaatgctgc gacgacacaa aaaccactag tagttctgaa agctattaac agctactatg
     4861 aacaggacaa tgccaatgtt caccgtggtg tccatacctt agcggaacga gcgacagctt
     4921 cttatgaagc tgctcgtgaa accattcgta agtttattaa tgcatgctct acaaaggaag
     4981 ttctctttac cagaggaacg acaaccagcc ttaactgggt ggcacgcttt gctgaggaaa
     5041 ttctcactga gggagaccag gtcttgattt cagtaatgga acaccattct aatatcattc
     5101 catggcagga agcttgtcga aagactggag cagagcttgt ctatgtctat cttaaagacg
     5161 gtgccttgga tatggaggat ttgcgagcta aattgactga taaggttaaa tttgtttccc
     5221 tagctcatgc ctccaatgtt cttggtgtgg tcaatccgat taaggaaatc actcaattag
     5281 cccaccaagt tggggcaatc atggtagtgg atggtgctca atctacacct catatgaaga
     5341 ttgatgtcca ggacttggat ctggactttt tcgccttttc gggtcacaag atggctggtc
     5401 cgactggtat cggtgtcctt tacggcaaag aaaagtatct tgagcaaatg tctccagtag
     5461 aatttggcgg cgagatgatt gattttgtct acgagcaatc cgctagttgg aaggaattgc
     5521 cttggaaatt tgaggctgga acgccaaata tggcaggagc tattggactt gcgactgcag
     5581 ttgattatct ggaaaagatt ggtatggatg ccgttgaagc tcatgaacag gaattgattg
     5641 cgtacgtcta tccaaaactg caggcaattg agggattgac catttacggt tctcaggatt
     5701 tggctcaacg ttcgggtgtt attgccttta acctaggtga tctccatcct cacgatcttg
     5761 cgacggctct ggattatgaa ggagtggctg ttcgtgctgg tcaccattgt gcgcaaccct
     5821 tgcttcagta tttggaagtc ccagcatcag ctcgtgcaag tttttatatc tacaatacca
     5881 aggcagattg cgacaaacta gtcgatgccc tacaaaagac aaaggagttt ttcaatggca
     5941 ctttctaaac tagatagcct ttatatggca gtggtagcag accattcgaa aaatccacat
     6001 caccaaggga agttagaaga tgctgagcaa atcagtctca acaatccgac ttgtggggat
     6061 gtcatcaacc tctctgtcaa gtttgatgca gaggaccgtt tggaagatat tgcttttcta
     6121 aattcaggat gcacgatttc aactgcttct gctagtatga tgacagatgt cgttttagga
     6181 aaaaccaaac aagaaatttt agaactggcg actatttttt ctgaaatggt tcaagggcaa
     6241 aaagatgagc gtcaaggcca acttggagac gcggcattct tgtcaggtgt tgccaaattc
     6301 cctcaaagaa tcaagtgtgc aaccctagct tggaatgccc ttaagaaaac aattgaaaat
     6361 caagaaaaac agtaagacaa gtttcttttg tcttatgaat tattagaaat gaagaaagaa
     6421 aggatactat ggctgaagaa agagtagaac caaaaccaat tgaccttggt gaatataaat
     6481 ttggtttcca tgacgatgta gagcctgtct tatcgacagg aaaaggactc aacgaaggtg
     6541 ttattcgtga attatctgct gctaagggtg agcctgagtg gatgttggag ttccgtttga
     6601 agtcttatga aaccttcaaa aaaatgccca tgcaaacttg gggagcagac ttgtcagaga
     6661 ttgactttga tgacttaatc tactaccaaa aaccatctga caaaccagcc cgttcttggg
     6721 atgatgtacc tgaaaagatt aaagaaacct ttgaacgtat cgggattcca gaagctgaac
     6781 gtgcttattt agcaggggct tctgcccagt acgagtcaga agtggtttac cacaacatga
     6841 aggaagagtt ccaaaaatta ggtattatct ttacagatac agattccgca ctcaaggaat
     6901 acccagactt atttaaacaa tactttgcga agttggtacc gccgacagat aacaagttgg
     6961 cagccctcaa ctcagcagta tggtcgggtg gaacttttat ctacgtgcca aaaggtgtca
     7021 aggtagatat tccacttcaa acttatttcc gtatcaataa cgaaaatata ggtcagttcg
     7081 aacgtacctt gattatcgtt gatgagggag caagcgtcca ctacgtagaa ggatgtacag
     7141 caccaacata ttcaagcaat agcttacacg ctgccattgt agaaattttt gctttggacg
     7201 gagcttatat gcgttataca actatccaaa actggtctga taacgtctat aacttggtaa
     7261 caaagcgtgc taaggctcaa aaggatgcca ctgttgagtg gattgatgga aacttgggtg
     7321 ccaaaacgac tatgaaatat ccatctgttt accttgatgg agaaggagcg cgtggtacca
     7381 tgctctctat cgcctttgct aatgcagggc aacaccaaga cacgggtgct aagatgattc
     7441 acaatgctcc acataccagc tcgtctattg tgtctaaatc catcgctaaa ggtggaggaa
     7501 aggttgacta ccgtggacaa gtcaccttta acaagaactc taagaaatct gtttcccaca
     7561 ttgaatgtga taccattatc atggatgact tgtcagcatc agatactatt ccatttaatg
     7621 aaattcacaa ctcgcaagta gctttggaac acgaagccaa agtatctaag atttcagaag
     7681 agcaattgta ttatctcatg agccgtggat tgtcagaatc tgaggcaact gaaatgattg
     7741 tcatgggatt tgtagaaccc tttacaaaag aacttccaat ggaatacgca gttgagctga
     7801 accgcttgat tagctatgaa atggagggat cagttggata aaatttgatt ttatactctt
     7861 cgaaaatctc ttcaaaccac gtcagcatcg ccttaccgta tgtatggtta ctgacttcgt
     7921 cagtttcatc tacaacctca aaacagtgtt ttgagcaacc tgcggctagc ttcctagtta
     7981 gttctttgat tttgagtatt agatttactc aaaatcaagg attttgaaga tgaacttgta
     8041 tcaaaaaatc gcggtttaaa atcgcgattt tttataattt ctcgttaaca aagcggacaa
     8101 actgattcca ccaaactttt aagaagaagg ctttttcaat tttcttgtct gctaccattt
     8161 cgaaactagg gcgctctgtg gtgatgtaac cttgaccaat caagtccttg tcttcataag
     8221 tcaaatggcc aaccactgtt ccagcttcaa gtggtgctgg gattgctttg gaatcaggtg
     8281 tgaattgaac agattgggaa gattgattcc caacacgttc gattagatag atatcctctg
     8341 gagccactgc agttactgta tcttcttttc catcttgtac aggggctttg ctatcttgat
     8401 aggcatcgcc ttgttgaacg attttgcgaa gtgtaaatgt agaagaaata taatccatta
     8461 gggaagatgt agctgtaaat cgagcgtaag gattattgtc ttgatgatct gcatttaaaa
     8521 caactgtgat gactctcatg cctttttcga cagtagtacc aacaaaagac tctccagcct
     8581 tatctgttgt tcctgttttt agcccatcaa aaccaccacg gtaagcaggc ataccttcta
     8641 acatgtagtt ggttgaagtg attgtcatcc cagcaaaagt agaagaaggt tttttggtga
     8701 tttctaagac ttgtgggtat tttttgatga ggttgcgagc aacgatagcg acatcataag
     8761 cactaagctt attttcctca tcttttttag aacctgggta aatgttatcc cctagagttt
     8821 cattgttaag acctgtcgta ttgacaacag tggcatcctg aattccccat tccaagagtt
     8881 ttgcccgcat catatcgacg aaatcttttt ctgagccagc aattttctca gctagggcaa
     8941 tagcggcgct gttggcacta gataccagag ttgcttcaag caactcttcg acagtataat
     9001 tacgggcctc cataggaata ttactggctt cagaatttgt cgtcaattga taaggataat
     9061 cagaaatatc tacaggagtg gagagggtaa tacttccgtt ttccaaagct tcatagacca
     9121 gataaacagt aatcaatttt gttatggaag caatttcgac aggttgcgtt gcatccttct
     9181 catagagaat tttaccagta tttgcctcaa cagcaatcgc atgtttagcg gcaatggtaa
     9241 aatcttgagc aacagcagta gaagcacccc ctaaaagaga gacagttaac aaagttaaaa
     9301 atattttttt catagtagtc ttattctatc ataaagaaaa aaaatattct tgctttaata
     9361 attcatctgt taagcttttt gaaaatatgg taaaataaag tgagggaggt aactcatgtt
     9421 tcgtagaaat aaattatttt tttggaccac agaaatttta ctcttaacca tcatctttta
     9481 tctatggaga cagatggggt ctttgattaa cccttttgtt agcgtgctta atacaattat
     9541 gattccattt ttattagggg gctttcttta ttatttgaca aaccctattg ttactttctt
     9601 aaataaagtc tgtaaactca atcgtttgct tggtatttta attaccttgt gtactttgat
     9661 ctggggaatg gtcataggtg ttgtctatct cttacctatt ttgattaatc agttatctag
     9721 tttgattata tctagtcaaa ctatttatag tcgagtacaa gacttaatca tagacttatc
     9781 taattatcct gcgctccaga atttggatgt agaagctaca attcagcagt taaacttatc
     9841 ctatgttgat attcttcaaa atatcctaaa tagcgtatca aatagtgtgg ggagcgtctt
     9901 gtcagctctt atcagtactg ttttgatttt gattatgact ccagtttttt tggtttattt
     9961 cttattagat ggacataaat tcttgcccat gcttgaaaga acgattctaa agagggatcg
    10021 cttgcatatt gcaggcttat taaagaattt aaatgcgacg attgctcgct atattagtgg
    10081 agtttcgatt gacgcaatca ttataggttg tttggcttat attggctata gtattattgg
    10141 tttaaaatat gctttagttt ttgccatttt ttctggtgta gccaatttaa ttccttatgt
    10201 ggggccaagt attggtttga ttcctatgat catcgcaaat atattcactg atccccatag
    10261 actgctgatt gcagtgattt atatgcttgt tgttcagcag gtagatggca atatcttata
    10321 tcctcgaatc gtaggaagtg ttatgaaggt tcatccaatc acgattttag ttttactttt
    10381 gttgtcaagc aatatctatg gtgtagttgg aatgattgtc gcagtgccaa cctattctat
    10441 cttgaaagaa atttctaagt tcttatcccg tttgtatgaa aatcataaaa taatgaaaga
    10501 acgagaaaga gaattagcta agtaaaagtc aggagaaccc tgatttttct ttactggaag
    10561 tggcctttag attagaagac tgaaaataag ttaaagtctt aaactaattt tcacagctaa
    10621 gaatagtaga agttaatctg ataaaaatcg aaaaaaccag tggaattccg tgtcagggta
    10681 agttccactg gttttcatag tctattaaag ttcgaatgaa acctatttat agtatattga
    10741 aactagaata gtacacctct aattctaaaa cattgttaga aatcgatttg actgtcctga
    10801 tctattcgtc ctattcttat ttcattttac tatattttgg tgcaataagt gaaaagtagt
    10861 ccgaataata taaggattga ttttatagtt tttaaactca aatgaattaa aataaagaga
    10921 gtacgaaaat tctcatctga aagtatttta gaataattct cttcgtgaat ttcttcaaaa
    10981 cagatagctt catcttaggt atgtgttaat tggatttcgt aagctctatt tacaatcgta
    11041 aagttatgca ctgaaaaagt tgtagttagt cttccagttt gctaaatttc tcgaatagta
    11101 tcttttttag gtatgtgatt tctttttgca tttttgagtt agataaggta taatggtttt
    11161 attgtctttt ggggtcgtta cggattcgac aggcattatg aggcatattt tgcgactcgt
    11221 gtggcgacgt aaacgctcag ttaaatataa ctgcaaaaaa taacacttct tacgctctag
    11281 ctgcctaaaa accagcaggc gtgacccgat ttggattgct cgtgttcaat gacaggtctt
    11341 attattagcg agatacgatt aagccttgtc tagcggtttg ataagagatt gatagactcg
    11401 cagtttctag acttgagtta tgtgtcgagg ggctgttaaa ataatacata acctatggtt
    11461 gtagacaaat atgttggcag gtgtttggac gtgggttcga ctcccaccgg ctccattatt
    11521 cctttgcatt ccttggtaaa acgttgttaa atcaacgttt tttattttta tctttggtat
    11581 tcctttgcat tcttttgcta aaaagggagt cacaaacaga ccctatttta aaaaaaggat
    11641 agaaaaaagg atacaacatt tgtcgcatcc taaaaataat cttttttcga cggaagacat
    11701 gggattcgaa cccacgcacg ctattacacg cctaccgcgt ttccaacacg gcctcttaag
    11761 cctcttgagt aatcttccaa tacttactca aatagtctac cataaaggct cttatcttgc
    11821 aataaaaatt ctagaaataa gaaaaaatga tagattttga aagaaaatga taaaaaatgc
    11881 ttgacttcga aagaaagtat ggtagaatga atagtgtaaa cgataacagg aggtgattca
    11941 gtgttaaaaa cagaacgtaa acaactgatt ttagaggagt taaatcaaca tcatgtagtt
    12001 tctctagaaa aattagttag tttgctagaa acgtcagaat caacggttcg aagagacttg
    12061 gatgagttgg aagcggaaaa caagcttcgt cgtgtgcatg gtggagcaga actcccccac
    12121 tccttacagg aagaagaaac cattcaagaa aaatctgtca aaaaccttca agaaaagaag
    12181 ttgctggctc agaaagcagc ctctctcatt aaagaaaaag atgtcatctt tatcgatgct
    12241 ggaacaacaa ctgctttttt gattcatgaa ttggtcaata agaatgttac agttgtgacc
    12301 aactccattc accatgccgc tcagttggtt gaaaagcaga ttccaactgt catggttgga
    12361 ggaaacgtca agacggcgac agatgctagt atcggtggcg ttgctcttaa ccagattaac
    12421 caattgcact ttgaccgtgc ctttatcgga atgaatggtg ttgacgatgg ctattatacg
    12481 actcctgata tggaggaggg agctgtgaaa agagctattt tggagaatgc caagcagacc
    12541 tacgtcttgg tggattcgtc aaaaattgga caaacttgct ttgccaaggt agcaccactc
    12601 aaacgcgcta tcgttatcac tagtcaaggg catgagctct tgcaggttat taaggagaaa
    12661 acggaggtaa tagaagtatg atttatacag tcacactcaa tccatccatt gactatatcg
    12721 ttcgtttgga ccaagtcaaa gttggtagtg taaatcgtat ggacagtgat gataagtttg
    12781 ctggtgggaa aggaatcaat gtcagccgtg tcttgaaacg tttgaatata ccaaatacag
    12841 cgacgggatt tatcggtggc tttactggta aatttatcac agatacttta gcagaggaag
    12901 aaatcgagac acgttttgtc caggtggcag aagatactcg tatcaatgtt aaaatcaaag
    12961 cagaccaaga aacagaaatc aacggaacgg gtccaactgt tgaatcggtt cagctagaag
    13021 aattgaaagc tattttatct agtctgacag cagaagatac agttgtcttt gcaggttcaa
    13081 gtgctaaaaa tctaggcaat gttatctata aggatttgat ttccttgacg cgccagactg
    13141 gtgcgcaagt ggtctgtgac tttgaaggac agaccttaat tgatagtttg gactaccagc
    13201 ctcttcttgt aaaaccaaac aatcatgaac ttggagcgat ttttggggtt aaactcgaaa
    13261 gtttagatga aattgagaaa tacgctcgtg agttactggc taagggtgct caaaatgtta
    13321 ttatctctat ggctggtgat ggtgcccttc ttgtcacatc tgatggagct tacttcgcta
    13381 aaccaatcaa gggaacagtc aaaaattcag ttggagctgg tgattctatg gttgctggat
    13441 tcacaggtga atttgtcaaa tcaaaagacg cagtagaagc cttcaaatgg ggagtggctt
    13501 gcggaacggc aactaccttc tcagatgact tggcaacggc ggaatttatt aaagaaacat
    13561 atggaaaagt tgaggtagaa aaacgatgaa aattcaagac ctattgagaa aagatgtcat
    13621 gttgctagat ttgcaggcaa ctgaaaaaac agctgtcatc gacgagatga ttaaaaattt
    13681 gacagaccac ggttatgtaa cagattttga aacatttaaa gaaggaattt tggcgcgtga
    13741 agctttgact tctactggtt tgggtgatgg aatcgcaatg cctcacagca aaaacgctgc
    13801 tgtcaaagaa gcgacagttc tatttgctaa gtcaaataag ggtgttgact acgagagctt
    13861 ggatggacaa gcaactgacc tcttcttcat gattgcagct ccagaaggtg ccaatgatac
    13921 tcacttggca gccttggcag aattgtctca atacttgatg aaagacggtt ttgcagacaa
    13981 acttcgtcaa gcaacatctg cagaccaagt tatcgaactt tttgaccaag cttcagaaaa
    14041 aactgaggaa cttgttcaag cacctgctaa tgactctggt gactttatcg tagctgttac
    14101 agcttgtaca acaggtattg cccacactta catggcccaa gaagcccttc aaaaagtagc
    14161 tgctgaaatg ggggttggta tcaaggtcga aaccaacggt gctagcggtg ttggaaatca
    14221 actaactgca gaagatatcc gtaaggctaa agctattatc attgcagcag acaaggccgt
    14281 tgaaatggat cgatttgatg gaaaaccatt gatcaatcgt ccagttgctg acggtatccg
    14341 taagacagaa gagctaatta acttggctct ttcaggagat actgaagtct accgtgccgc
    14401 taatggtgcc aaagctgcaa cagcctctaa cgaaaaacaa agccttggtg gtgccttgta
    14461 caaacacttg atgagtggtg tatctcaaat gttaccattc gttatcggtg gtggtatcat
    14521 gattgccctt gccttcttga ttgacggtgc tttgggtgtt ccaaatgaaa accttggcaa
    14581 tcttggttct taccatgagt tagcttctat gttcatgaaa attggtggag ctgcctttgg
    14641 tttgatgctt ccagtctttg cgggttatgt tgcctactct attgctgaaa aaccgggttt
    14701 ggtagcaggt ttcgtggctg gtgctattgc caaagaaggt tttgcctttg gtaaaattcc
    14761 ttatgccgca ggtggtgaag caacttcaac tcttgcaggt gtctcatctg gtttcctagg
    14821 tgcccttgtt ggtggattta tcgcaggtgc cttggttctt gccatcaaga aatacgttaa
    14881 agttcctcgt tcactcgaag gtgctaaatc aatccttcta ttgccacttc ttggaacaat
    14941 cttgacagga tttgttatgc tagctgtgaa tatcccaatg gctgcaatca acactgctat
    15001 gaatgacttc ctaggcggtc ttggaggagg ttcagctgtc cttcttggta tcgtccttgg
    15061 tggaatgatg gctgttgaca tgggtggacc agttaataaa gcagcttatg tctttggtac
    15121 aggtacgctt gcagcaactg tttcttcagg tggttctgta gccatggcag cagttatggc
    15181 tggaggaatg gtgccaccac ttgcaatctt tgtcgcaact cttcttttca aagataaatt
    15241 tactaaggaa gaacgtaact ctggtttgac aaacatcatc atgggcttgt catttatcac
    15301 tgagggagcg attccatttg gtgccgctga cccagctcgt gcgattccaa gcttcatcct
    15361 tggttcagca gtagcaggtg gactcgttgg tcttactggt atcaaactca tggcgccaca
    15421 cggaggaatc ttcgttatcg cccttacttc aaatgctctc ctttacctcg tttctgtctt
    15481 ggtaggagca atcgtaagtg gtgtggttta tggttaccta cgcaaaccac aagcataaaa
    15541 aatagaaaaa tgaaaagatt ggaccgtttg gtgcagtctt tttctcttcc cgaaatgcct
    15601 gtgaaatatg gtataataga agaatggcaa acaagaatac aagtacaaca agacggagac
    15661 cgtctaaagc agaactggaa agaaaagaag cgattcaacg aatgttgatt tcgttaggaa
    15721 ttgcgatttt attgattttc gcagccttca aattaggggc tgcaggtata accctttata
    15781 atttaattcg cttgctagtg ggtagcctag cttatctggc gatattcggc ctattaatct
    15841 atctcttctt tttcaagtgg atacgaaaac aggaaggact cttatctggc tttttcacca
    15901 tatttgctgg cttactcttg atttttgagg cctacttggt ttggaaatat ggtttggaca
    15961 agtccgttct aaaagggacc atggctcagg ttgtgacaga tctgactggt tttcgaacga
    16021 ctagctttgc tggagggggc ttgatcgggg tcgctcttta tattccaaca gcctttctct
    16081 tttcaaatat cggaacttac tttattggtt ctatcttgat tttagtgggt tctctcctag
    16141 tcagcccttg gtctgtttac gatattgctg aatttttcag tagaggcttt gccaaatggt
    16201 gggaagggca cgagcgtcga aaagaggaac gctttgtcaa acaagaagaa aaagctcgcc
    16261 aaaaggctga gaaagaggct agattagaac aagaagagac tgaaaaagcc ttactcgatt
    16321 tgcctcctgt tgatatggaa acgggtgaaa ttctgacaga ggaagctgtt caaaatcttc
    16381 cacctattcc agaagaaaag tgggtggaac cagaaatcat cctgcctcaa gctgaactta
    16441 aattccctga acaggaagat gactcagatg acgaagatgt tcaggtcgat ttttcagcca
    16501 aagaagccct tgaatacaaa cttccaagct tacaactctt tgcaccagat aaaccaaaag
    16561 atcagtctaa agagaagaaa attgtcagag aaaatatcaa aatcttagaa gcaacctttg
    16621 ctagctttgg tattaaggta acagttgaac gggccgaaat tgggccatca gtgaccaagt
    16681 atgaagtcaa gccggctgtt ggtgtaaggg tcaaccgcat ttccaatcta tcagatgacc
    16741 tcgctctagc cttggctgcc aaagatgtcc ggattgaagc accaatccct gggaaatccc
    16801 taatcggaat tgaagtgccc aactccgata ttgccactgt atctttccga gaactatggg
    16861 aacaatcgca aacgaaagca gaaaatttct tggaaattcc tttagggaag gctgttaatg
    16921 gaaccgcaag agcttttgac ctttctaaaa tgccccactt gctagttgca ggttcaacgg
    16981 gttcagggaa gtcagtagca gttaacggca ttattgctag cattctcatg aaggcgagac
    17041 cagatcaagt taaatttatg atggtcgatc ccaagatggt tgagttatct gtttacaatg
    17101 atattcccca cctcttgatt ccagtcgtga ccaatccacg caaagccagc aaggctctgc
    17161 aaaaggttgt ggatgaaatg gaaaaccgtt atgaactctt tgccaaggtg ggagttcgga
    17221 atattgcagg ttttaatgcc aaggtagaag agttcaattc ccagtctgag tacaagcaaa
    17281 ttccgctacc attcattgtc gtgattgtgg atgagttggc tgacctcatg atggtggcca
    17341 gcaaggaagt ggaagatgct atcatccgtc ttgggcagaa ggcgcgtgct gcaggtatcc
    17401 acatgattct tgcaactcag cgtccatctg ttgatgtcat ctctggtttg attaaggcca
    17461 atgttccatc tcgtgtagca tttgcggttt catcaggaac agactcccgt acgattttgg
    17521 atgaaaatgg agcagaaaaa cttcttggtc gaggagacat gctctttaaa ccgattgatg
    17581 aaaatcatcc agttcgtctc caaggctcct ttatctcgga tgacgatgtt gagcgcattg
    17641 tgaacttcat caaggctcag gcagatgcag actacgatga gagttttgat ccaggtgagg
    17701 tttctgaaaa tgaaggagaa ttttcggatg gagatgctgg tggtgatccg ctttttgaag
    17761 aagctaagtc tttggttatc gaaacacaga aagccagtgc atccatgatt caacgtcgtt
    17821 tgtcagttgg atttaaccgt gcgacccgtc tcatggaaga gcttgagatg gcaggtgtca
    17881 ttggtccagc tgaaggtacc aaaccaagaa aagttttaca acaataaaaa aatagcttct
    17941 ttccaagttt ggaaggaggc tattttagtg gttattgatt acttttattt tctgaagttg
    18001 gagtattggg ctgtttttca ttttcagtgg caggtttact tgtagcaggt gtagtagagt
    18061 cctgagttgc tgttttctga tcttcttttt tctcttcctt gacgctagat tttggtgttt
    18121 cctcttgctg tgttttttct tgactagtgt tagtctcttt agttggactg gtgttttcct
    18181 taggggattc cttttggatt tctttgacaa tggttgtcgt ctggcttgtc gtaggttctt
    18241 ttttaatatt tttgttatta tccaaggcgt tcatgatcgt gatactcgcg gtaatcaagc
    18301 caaggatact agcgattgta gcaacggttt tttgaaagac tgtaaagcct tttttgatgt
    18361 gttctgtttt tggttttgaa gttctacgat agttagacat acattctctc ctttgattat
    18421 gatttattat actctgtttc tttaaaaaaa tcttaaaaaa agagaaattg ccctttcttg
    18481 tcgcaagctc cgtttcatga tacaatagag gaagtgattt tagaaagcgt gagaaacatg
    18541 atttactttg ataattcagc gacgaccaag ccttatcctg aagcccttga aacctatatg
    18601 caggtcgctt caaaaatttt aggaaaccca tctagtctcc atagtttggg agaccaagca
    18661 acacgaatct tagatgcttc tcgccaacag attgcagatt taatcggtaa gaaaagcgat
    18721 gaaatcttct ttacctctgg tggaacagaa ggggataact gggttatcaa gggtgtggcc
    18781 tttgaaaaag ctcagtttgg caagcacatc attgtttcag ccattgaaca tccagcagtc
    18841 aaagagtcag ccctctggtt gaaaagtcaa ggatttgaag tggattttgc tccagttgat
    18901 aacaaaggat ttgtggatgt tgaggcgcta gaagatttga taagacctga tacgaccctc
    18961 gtttccatca tggctgtgaa caatgaaatc ggctctatcc aacctattga ggctatttca
    19021 gaattcttgg cagacaagcc aactatttcc ttccacgttg atgcggttca ggcgcttgcc
    19081 aagattccga ctgaaaagta tctgacagaa cgagtggatt tcgcgacttt ctctggtcac
    19141 aagttccacg gaatccgtgg tgttggtttt atctatatca agtctggcaa gaagattacg
    19201 cctcttttaa caggtggtgg tcaggaacgt gattaccgtt cgacaactga aaatgtggca
    19261 ggaattgcag cgaccgctaa ggctctccgt ttatctatgg aaaagctaga tatctttagg
    19321 agcaagactg ggcagatgaa ggcagtgatt cgtcaagcac ttctggacta tccggatata
    19381 tttgtctttt cagatgagga agacttcgcc cctcatattc tgacttttgg aatcaagggt
    19441 gttcgtggtg aggtcattgt tcacgccttt gaagactatg atattttcat ctcaacgacc
    19501 tcggcttgtt cgtccaaggc tgggaaacca gccggaaccc tgattgctat gggagtggac
    19561 aaggataagg ctcagtcagc tgtgcgtctt agtctagacc ttgaaaatga tatgagtcag
    19621 gtcgagcagt tcttgaccaa gctaaaatta atttacaatc aaactagaaa agtaagatag
    19681 gagcattcat gcagtattca gaaattatga ttcgctacgg tgagctgtca accaagggca
    19741 aaaatcgtat gcgtttcatc aataaacttc gcaataatat ttcagacgtt ttgtctatct
    19801 atccccaagt taaggtaaca gcagatcgcg accgtgccca cgcttacctc aatggagcag
    19861 attacacagc agtagcagaa tcgctcaaac aagtatttgg aattcaaaac ttttcccctg
    19921 tttataaggt tgaaaaatct gtagaagttc tgaagtctgc tgtccaagag attatgcagg
    19981 acatctacaa ggaaggcatg acctttaaaa tctctagcaa gcgtagcgac cacacctttg
    20041 aattggacag tcgtgaactc aaccaaacgc ttggaggggc tgtattcgaa gccattccaa
    20101 acgtacaagc tcaaatgaaa agtcctgaca tcaatcttca ggtggagata cgtgaagaag
    20161 cagcctatct ttcttatgaa accgttcgag gggcaggtgg attaccagtg ggaacttctg
    20221 gtaagggcat gctcatgttg tcagggggaa tcgattcccc tgtagctggc tatctagcgc
    20281 ttaaacgagg ggtagatata gaggcagttc actttgccag cccaccttac acgagtcctg
    20341 gtgctcttaa aaaagcccaa gatttgaccc gtaaattaac caaatttggg ggaaatatcc
    20401 agtttatcga agttcctttt acagagattc aagaggaaat caaagccaaa gcgccagaag
    20461 cctaccttat gaccttgacg cgtcgtttta tgatgcgtat tactgaccgt attcgtgagg
    20521 tacgaaatgg tttagttatc atcaatgggg aaagtctagg tcaagtagcc agccaaaccc
    20581 ttgaaagtat gaaggctatc aatgctgtta ccaacactcc catcattcgt cctgtggtta
    20641 ccatggacaa gttggaaatc attgacatcg cccaggaaat cgataccttt gacatttcaa
    20701 tccaaccgtt tgaagactgt tgtaccattt ttgcaccaga tcgtccaaaa acaaatccta
    20761 aaattaagaa tgcggagcag tacgaagcgc gtatggatgt tgaaggcttg gttgagcgag
    20821 cagtggctgg aatcatgatt actgaaatca cacctcaagc cgaaaaagat gaagttgatg
    20881 acttgattga caatctgctc taattcagaa aatccaaaag aatagcgaaa atcagtaaaa
    20941 aaagttagtt ttttctctaa aaacaggtaa aaaactaact ttttttattt ttatgatata
    21001 atgatataaa attttgaata tagagagttt tctgacaatg aatcaatcct acttttatct
    21061 aaaaatgaaa gaacacaaac tcaaggttcc ttatacaggt aaggagcgcc gtgtacgtat
    21121 tcttcttcct aaagattatg agaaagatac agaccgttcc tatcctgttg tatactttca
    21181 tgacgggcaa aatgttttta atagcaaaga gtctttcatt ggacattcat ggaagattat
    21241 cccagctatc aaacgaaatc cggatatcag tcgcatgatt gtcgttgcta ttgacaatga
    21301 tggtatgggg cggatgaatg agtatgcggc ttggaagttc caagaatctc ctatcccaga
    21361 gcagcagttt ggtggtaagg gtgtggagta tgctgagttt gtcatggagg tggtcaagcc
    21421 ttttatcaag cacaaaacag gttggtttga tggcatgatg acgacaggtt gctctatggg
    21481 agcctatcat gcactcaatt tcttcctcca gcatccagat gtctttacca aagtgattgc
    21541 tctcagtggt gtttacgacg cacgtttctt tgtcggtgat tactacaacg atgatgctat
    21601 ttaccaaaac tcgccagtag attatatttg gaaccaaaac gatggctggt ttattgaccg
    21661 ttaccgtcag gcagagattg tgctgtgtac ggggcttgga gcctgggaac aagatggttt
    21721 accatccttt tacaagctca aagaagcctt tgaccagaaa caaattccag cctggtttgc
    21781 tgaatgggga catgatgtcg cccacgactg ggaatggtgg cgtaaacaaa tgccttattt
    21841 cctcggtaat ctctatttat aaaaggagtt acctatgaat taccttgtta tttctcccta
    21901 ctatccacaa aactttcaac agtttaccat cgaactagct aataaaggca tcacagtctt
    21961 gggaattggt caagagtctt acgagcaatt ggatgagccc ttgcgcaata gcttgaccga
    22021 gtattttcgt gttgataatc ttgagaacat agatgaagtc aaacgtgcag ttgcttttct
    22081 cttttataaa catggtccaa ttgaccgcat cgagtctcac aatgaatact ggcttgagct
    22141 agacgcaaca ctcagagaac aattcaatgt ttttggtgcc aaaccagagg atctcaaaaa
    22201 gacgaaatat aagtctgaaa tgaagaaact tttcaaaaaa gcaggtgttc ctgtggtacc
    22261 tggagctgtt atcaagacgg aagcagatgt tgatcaagca gtgaaagaaa tcggtcttcc
    22321 aatgattgcc aaacctgata atggagtggg agcagccgca acctttaaac ttgagacaga
    22381 agacgatatc aatcacttca agcaagaatg ggaccattca accctttatt tctttgaaaa
    22441 atttgtcact tccagcgaaa tctgtacctt tgacgggctc gtggacaagg atggaaagat
    22501 tgtcttctca acaacctttg actacgccta tacaccgctt gacctcatga tttataagat
    22561 ggacaattct tattatgtgc tcaaggatat ggatcctaaa ctgcgcaagt atggggaagc
    22621 aattgtcaaa gaatttggta tgaaagaacg gtttttccat attgagttct tccgtgaggg
    22681 ggacgattat attaccatcg agtacaataa ccgccctgca ggtggtttta ccattgatgt
    22741 ttataacttt gctcattcct tggaccttta tcgtggctat gcagctattg tcgcaggaga
    22801 ggagttcccg gcgtcagact ttgaaactca gtattgtttg gctacttctc gccgtgcaaa
    22861 tgctcactat gtttattcag aagaggattt gcttgccaaa tatagccagc agttcaaggt
    22921 taaaaaagtc atgccagctg ccttcgcgga acttcaagga gattacctgt atatgctgac
    22981 aactccgagt cgacaagaaa tggatcagat gattgctgat ttcggacaac gtcaagaata
    23041 agaactatcg gattaaggaa attcactccc ttaatccttt tgttttgtct ggtaaaaaat
    23101 aagagcatcc taacaaggta gctatcataa aacttgttcg ataactattt gaagcaggat
    23161 taggtggcta gaaattgaat tttaacattt caatttagtc atagtattgt gttttcgtat
    23221 ccttaaatca gctaaaagga tccatgacga cacctatacg atatagtttt caagatacca
    23281 aacaagtcta ttaatattca atgaaaatca aagagcaaac taggaagcta gccgcaggtt
    23341 tctcaaaaca ctgttttgag gttgtggata gaactgacag agtcagtatc atatacctac
    23401 ggcaaggtga agctgacgtg gtttgaagag attttcgaag agtataaaat attcaggtga
    23461 cgcatagata tagttaattg aagctttgtt tgaaatctga taaaataatg atattactaa
    23521 gttttaaaaa ctaaagaaaa gggaagatat gattacaggc gaattaaaaa ataaaatcga
    23581 tcagctgtgg gaaattcttt ggacagaagg aaacgcaaat cctttaacaa atattgaaca
    23641 gttgacttat ctcttattta tgaaagattt ggatagtgtc gagcttggac gtgaaagtga
    23701 tgctgaattt ctagggattc cttatgaggg agtttttcca aaagataaac ctgaataccg
    23761 ttggtcaact tttaaaaata taggagatgc tcaggaagtt tatcgtttaa tgactcagga
    23821 gatttttccg tttattaaaa atctcaaggg ggatacagat gatacagcct tttcacgata
    23881 tatgcgagaa gctatttttc aaataaataa acctgctacg cttcaaaagg caatttctat
    23941 cttagatgtt tttccaacta ggggattaga tgtagatttt gataatgaca aacaaagtat
    24001 tactgatatc ggagatatct atgaatatct gttatcaaaa ttgtcgaccg caggtaaaaa
    24061 tggacagttc cgtacacctc gtcacatcat cgatatgatg gttgagttga tgcaaccgac
    24121 tatcaaagat atcatctcag atcccgctat gggttctgct ggcttcttag tatctgctag
    24181 ccgttactta aagcgtaaga aagatgaatg ggaaaccaat acagataata tcaatcattt
    24241 tcataatcag atgtttcatg gaaatgatac ggatacgact atgttgagac ttggggcgat
    24301 gaacatgatg ctacatggag tagaaaatcc acaaatcagt taccttgact cgctgtctca
    24361 agataatgaa gaagccgata aatatacttt ggttttagca aatcctcctt ttaagggctc
    24421 acttgactac aattcaacct ctaatgacct tcttgcaacc gtaaaaacca aaaaaacaga
    24481 attactcttt ctttctcttt tcttgcgaac tttaaaacca ggtggacgag cagcagttat
    24541 cgtacctgat ggtgtccttt ttggttcgtc taaagctcat aaaggaattc gtcaggaaat
    24601 tgtagagaat cataagcttg atgctgtaat ctcaatgcct agtggtgtgt tcaagcctta
    24661 tgctggagtt tcaactgcca ttctcatctt tacaaaaact ggtaatggtg gtactgacaa
    24721 agtctggttt tacgatatga aagcggatgg tttaagtttg gatgataagc gacaaccgat
    24781 tagcgacaat gatattccag atattatcga acgctttcat catcttgaaa aagaagcaga
    24841 acgtcagaga acggatcaat ctttctttgt tccagttgct gagataaagg aaaatgatta
    24901 tgatttgtct atcaataaat ataaagagat tgagtatgaa aaagttgagt atgaaccaac
    24961 agaggtcatc ttaaagaaaa tcaatgattt agaaaaagaa attcaagctg gcttggctga
    25021 attagaaaaa ttactcaagt agagaggtgg ctgtatgaaa aaagtgaagt tgggggaagt
    25081 cttatctcta aaaaaaggca agaaagccac tgtacttgct gaacaaacaa ctct
//
LOCUS       DAVLYV010000018        24624 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLYV010000018 DAVLYV010000000
VERSION     DAVLYV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.018"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            341..1504
                     /locus_tag="VYO70_000814"
     CDS             341..1504
                     /locus_tag="VYO70_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1713079.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVTFNQTFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGFDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLTMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1501..2178
                     /locus_tag="VYO70_000815"
     CDS             1501..2178
                     /locus_tag="VYO70_000815"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1713080.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            2168..3022
                     /locus_tag="VYO70_000816"
     CDS             2168..3022
                     /locus_tag="VYO70_000816"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1713081.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATDTNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3041..4108
                     /locus_tag="VYO70_000817"
     CDS             3041..4108
                     /locus_tag="VYO70_000817"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1713082.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4118..5284
                     /gene="aroC"
                     /locus_tag="VYO70_000818"
     CDS             4118..5284
                     /gene="aroC"
                     /locus_tag="VYO70_000818"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1713083.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5294..6397
                     /locus_tag="VYO70_000819"
     CDS             5294..6397
                     /locus_tag="VYO70_000819"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1713084.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6408..6746
                     /locus_tag="VYO70_000820"
     CDS             6408..6746
                     /locus_tag="VYO70_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1713085.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6838..8121
                     /gene="aroA"
                     /locus_tag="VYO70_000821"
     CDS             6838..8121
                     /gene="aroA"
                     /locus_tag="VYO70_000821"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1713086.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEICGALIPRLIDELPIIALLATQAQDVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVAGGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8114..8590
                     /locus_tag="VYO70_000822"
     CDS             8114..8590
                     /locus_tag="VYO70_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1713087.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     QRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8587..9435
                     /gene="pheA"
                     /locus_tag="VYO70_000823"
     CDS             8587..9435
                     /gene="pheA"
                     /locus_tag="VYO70_000823"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1713088.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9432..10706
                     /gene="psr"
                     /locus_tag="VYO70_000824"
     CDS             9432..10706
                     /gene="psr"
                     /locus_tag="VYO70_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1713089.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            10985..11830
                     /locus_tag="VYO70_000825"
     CDS             10985..11830
                     /locus_tag="VYO70_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1713090.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKKT
                     TCIKPYQQDLDLPHGLALDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSCILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVSHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11820..12923
                     /locus_tag="VYO70_000826"
     CDS             11820..12923
                     /locus_tag="VYO70_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1713091.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLYQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12925..13911
                     /locus_tag="VYO70_000827"
     CDS             12925..13911
                     /locus_tag="VYO70_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1713092.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATDLSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNSIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13927..15360
                     /locus_tag="VYO70_000828"
     CDS             13927..15360
                     /locus_tag="VYO70_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1713093.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGVLARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQAEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGILFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15389..16423
                     /locus_tag="VYO70_000829"
     CDS             15389..16423
                     /locus_tag="VYO70_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1713094.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPEDSAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16623..17360
                     /gene="mecA"
                     /locus_tag="VYO70_000830"
     CDS             16623..17360
                     /gene="mecA"
                     /locus_tag="VYO70_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1713095.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17511..18797
                     /locus_tag="VYO70_000831"
     CDS             17511..18797
                     /locus_tag="VYO70_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1713096.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18799..19668
                     /locus_tag="VYO70_000832"
     CDS             18799..19668
                     /locus_tag="VYO70_000832"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1713097.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19744..19821
                     /locus_tag="VYO70_000833"
                     /pseudo
     CDS             19744..19821
                     /locus_tag="VYO70_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            19963..20901
                     /gene="msrB"
                     /locus_tag="VYO70_000834"
     CDS             19963..20901
                     /gene="msrB"
                     /locus_tag="VYO70_000834"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1713098.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            20961..22685
                     /locus_tag="VYO70_000835"
     CDS             20961..22685
                     /locus_tag="VYO70_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713099.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNRSYISAKRIKEVFVEAPEDIHSELEQKQVTSDRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIASVPQKVELFKGTIRSNLTLGFNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22687..24435
                     /locus_tag="VYO70_000836"
     CDS             22687..24435
                     /locus_tag="VYO70_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713100.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPIFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTHLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
BASE COUNT         7224 a         4606 c         5571 g         7223 t
ORIGIN      
        1 ataaaatcaa attttccaga tatctactga agctgattta tttcttcatc tttcttctac
       61 tacctttgtc actaaatttg acagacacat attgctgata caacctctct acctataatc
      121 tcacagttaa cctgaggcta gtttcatagt ttgctatttg attttcgttg ataataaaaa
      181 atgctcctcc aagacggaag agcatttttt attgtgattc ttacacattt acttcctagt
      241 tttgcaggaa gtctagtata ccatttttta aaagaagagt aaaaaagcaa gggtagtttc
      301 ttttttgaga attgtctaaa agtttgatat aatggtagtt atgaatagaa taagagtcag
      361 caaaagggtt gaaaagaagc ttgctaaggg gctagtttta ctagaagcca gtgatcttga
      421 gaatgtcaat cttaaggatc aggaagtaga ggtgcagggt caggaaggaa actttcttgg
      481 gactgcctac ctttctcagc aaaacaaggg cttgggctgg tttgtcagca aagacaaggt
      541 gaccttcaat caaactttct ttgaaacgtt gtttagaaaa gccaaagaaa agagaaacgc
      601 ctactatcaa gatgatttga caactgcctt tcgtctcttt aatcaagagg gagatggctt
      661 tgggggtctg acagtggacc tttatggcga ctacgccgtc ttttcttggt ataactctta
      721 tgtttatcag attcgtcaga ctatatcaga agcctttaga caggttttcc ctgaggtttt
      781 aggggcttat gagaaaatcc gctttaaggg ttttgactat gaatctgccc atgtttatgg
      841 tcaagaagca cctgactttt tcactgtttt agaaaatggt gtcctgtatc aagtctttat
      901 gaatgatggc ttgatgacag gaattttcct agaccagcat gaggttcgcg gtagtttagt
      961 tgacggcttg actatgggta aatccttgct caatatgttt tcctacacag cggctttttc
     1021 agtagctgcg gccatgggag gagctagcca gacaacttct gttgatctag ccaaacgttc
     1081 acgagaattg tctcaagcgc attttcaggc aaatgggctc agcacagacg agcatcgttt
     1141 tatagtcatg gatgtctttg agtatttcaa atatgccaaa cgcaaagact tgacctacga
     1201 tgtgattgtc ctagatccgc ctagctttgc tcggaataaa aaacaaactt tctctgtggc
     1261 caaggattat cacaagttga tttcccagag tcttgagatt ttaaatccgg gagggattat
     1321 cattgccagt accaatgctg ccaatgtttc ccgccagaaa tttacagaac aaattgataa
     1381 aggttttgca ggaagaagtt accagatttt aaacaaatat ggtcttccag cagattttgc
     1441 ctataataaa aaagatgaaa gtagtaatta cctcaaggtg attagtatga aggttagtaa
     1501 atgaaattaa tcgtttcagt aatgccaaga agtttagagg aggctcaggc tctggatgcc
     1561 acgaggtatc tggatgccga catcattgaa tggcgtgccg actatctgcc taaagaagcg
     1621 attttgcagg tagctccagc catttttgaa aaattcgcag gccgtgagtt ggttttcacg
     1681 ctacgaactc gctccgaagg tggagaaatc gacctttctc cagaagagta tatccatcta
     1741 atcaaggaag ttgcgcaact ctatcaacca gactatattg attttgagta ctatagctac
     1801 aaggatgttt ttgaggaaat gctggacttc ccaaatcttg ttttgagtta ccataatttc
     1861 caagaaacac ctgagaacat gatggaaatc ttgtcagagt tgacgatcct aaatccaaaa
     1921 cttgttaagg ttgcggtgat ggctcacacg gagcaggatg tcttagactt gatgaactat
     1981 acacgaggct ttaaaaccct caatcctgaa caggaatatg tgaccatttc tatgggtaag
     2041 gtgggcaagg tctctcgtat cactgcggat gtgactggtt cgagttggtc ttttgccagt
     2101 ctggatgagg ttagtgcccc tgggcagatt tctctagcta gcatgaaaaa aatcagggag
     2161 attttggatg aagcttgatg gctatacacg tttagctgca gttgttgcca atcctattaa
     2221 gcattctatt tctcccttca tccacaatag agcctttgag gcgacagata ccaacggtgc
     2281 ttatgtggct tgggagattg aagcgagtga cttggtagaa acagtggcca atattcgtcg
     2341 ctaccagatg tttggcatca atctgtccat gccctataag gagcaggtga ttccttattt
     2401 ggataagctg agcgatgaag cgcgcttgat tggtgcggtt aatacggttg tcaatgagaa
     2461 tggcaattta attggatata atacagatgg caagggattt tttaagtgct tgccttcttt
     2521 tacaatttca ggtaaaaaga tgaccctgct gggtgcaggt ggtgcggcta aatcaatctt
     2581 ggcacaggct attttggatg gcgtcagtca gatttcggtc tttgttcgtt ccgtttctat
     2641 ggaaaaaaca agaccttacc tagacaagtt acaggagcag acaggcttta aagtggattt
     2701 gtgtgcttta gaatatgttt ctgaactgca agcaaggatt gccgagtcgg atttgctagt
     2761 taatgccacc agtgtgggca tggatggcca atcctctcct gttcctgaaa acatagtctt
     2821 accagaaact ctcttagttg cagatatcat ataccaaccc tttgaaacac cgtttttgaa
     2881 atgggctaga agtcagggca atccagcagt caatggtctg ggaatgttac tctatcaagc
     2941 tgcagaagct tttcaactgt ggacaggcaa ggaaatgccg acagaagaga tttggcagtc
     3001 tttaacagaa aaataccaat aaagaaaggg agactctcct atgaaaatca gaatcgatat
     3061 tcctcatcat ccttatgata ttcagattga aaaaggttgt atggcccagg ctggtcagtg
     3121 gttgcgagaa ctctggcaac cgcaaaaggt agtcattgtg acagataacc atgtagcctc
     3181 tctctatgca gagaaggtca agctcagcct agaagatgct ggttttcagg tagctgtttt
     3241 tgatttctta gaaggtgaag aaagaaagaa tttaactact gttcagaaag tctatgaatt
     3301 tctagtcaag caaggtctga ctcgtagcga tggaatcgtt gctcttggtg gtggcgttgt
     3361 tggggacctg gctggctttg tagcctctac ctatatgcgg ggtattcact ttgttcagat
     3421 tccgactagt ttgacagctc aggttgattc ttctatcggt ggaaagacag gcgttaacac
     3481 accatttgct aaaaatatgg tggggacctt tgcccaacca gatggggttc tgattgatcc
     3541 acttgttctt gaaaccctcg gaaaaagaga gttgattgaa gggatgggtg aagttatcaa
     3601 gtatggcttg attgaggatc cagaactgtg ggctctcttg acgggactga atggttctgt
     3661 tgagagtatt ttggaacatg cagagacctt gattgaacat tcttgtcagg tgaagcgcaa
     3721 gatggtggtt gaagatgagt tggacaatgg tattcgtctt tacctcaatt ttggccacac
     3781 tattggccat gccatcgaag cgactgccgg ttatggcaag gtcatgcatg gagaggctgt
     3841 tgccatggga atggtacaga tttccaaggt tgctgaggaa aaaggcctca tgccagctgg
     3901 cattacccaa tctatcacag agatgtgtca gaaattcggc ttgcctgttg actatgaaaa
     3961 ttgggaagtt gacaagcttt atcaggctct tactcatgac aagaaagcgc gtggtaacac
     4021 cttgaaattg gtcttggtgc cagagcttgg ttcagcgatc attcacccag tttctctgga
     4081 agagatgaaa gactacttgg taaaataagg agagtgtatg agatatttaa ctgcaggaga
     4141 atcacacggc ccccgtctaa cagctattat tgagggaatc ccagctggac ttccattgac
     4201 agctgaggat atcaatgagg accttagacg ccgtcagggt ggctacggtc gtggtggtcg
     4261 tatgaagatt gagagtgacc aggttgtctt tacttcgggc gttcgccacg ggaagacgac
     4321 aggggcgcct attactatgg atgtcatcaa taaggaccac cagaaatggc tggacatcat
     4381 gtctgcggag gacattgaag accgccttaa aagcaagcgg aaaattactc atcctcgccc
     4441 aggtcatgcc gatttggttg gggggattaa gtaccgtttt gatgatttgc gaaattcttt
     4501 ggagcgttca tcagctcgtg aaaccaccat gcgggtggca gttggtgcag tagccaaacg
     4561 cctcttggct gagctggata tggagattgc caaccatgtc gtggtctttg gtggtaagga
     4621 aatagatgtt cctgaaaatc tgacagtcgc tgaaattaag caaagagctg cccagtctga
     4681 agtttctatt gtcaaccaag aacgagaaca ggaaatcaag gactatattg accaaatcaa
     4741 acgtgatggt gataccatcg gtggggttgt ggagacagtc gtcggaggcg ttccagttgg
     4801 tcttggttcc tatgtccaat gggatagaaa attggatgca agattggctc aagctgttgt
     4861 ctctatcaat gcctttaaag gggtggaatt tggtcttggc tttgaggctg gttatcgtaa
     4921 aggcagccaa gttatggatg aaattctctg gtctaaagaa gacggttata ctcgccgtac
     4981 caataatcta ggtggttttg aaggtggtat gactaatggg caacccatcg ttgttcgtgg
     5041 ggtcatgaaa cccattccta ctctttataa acctcttatg agtgtggata tcgaaaccca
     5101 cgaaccttac aaggcaaccg tggagagaag tgatccgact gctcttccag ctgcaggaat
     5161 ggtcatggaa gcagttgtag caacggttct ggcgcaagaa atcctcgaaa aattctcatc
     5221 agataatctt gaggaactaa aagaagcggt agccaaacac cgagactata caaagaacta
     5281 ttaaggagtt cctatggcaa aaacaatcta tatcgcaggt cttgggttga ttggagcctc
     5341 tatggcactt ggtatcaaac gcgatcatcc agattatgaa attttaggtt ataatcgtag
     5401 tcaagcttcg agagatatcg ccttgaaaga aggtatgatt gaccgtgcaa cggatgattt
     5461 tgctagtttt gctcctttgg cagatgtcat tatcctcagc ttgccaatca aacaaactat
     5521 tgctttcatt aaggagttag ccaatttgga tttgcgagaa ggcgttatta tttcagatgc
     5581 tggttcgacc aagtcaacca ttgtggatgc ggcggagcag tatttggctg gcaagtctgt
     5641 tcgctttgtc ggggcccatc ccatggctgg tagtcacaag acaggggctg cttcggcaga
     5701 tgtcaatctt tttgaaaatg cctattatat ctttacacct tcaagcctga caagtcagga
     5761 cacgcttaag gaaatgaagg atctgctttc aggtcttcat gctcgtttta tcgagattga
     5821 tgccaaggag catgatcgtg tcacttctca gattagccat ttccctcata ttttggcttc
     5881 tagtctcatg gagcagactg cggtctatgc tcaagagcat gagatggcaa ggcgctttgc
     5941 ggcaggtggt tttcgagata tgacccgaat tgcggaaagc gagccaggaa tgtggacctc
     6001 cattctcttg tccaatagcg agaccattct ggatagaatt gaggatttca aggaacgttt
     6061 ggaagtgatt ggtcaggcca ttagtaaggg agatgaagag caaatttgga acttttttaa
     6121 ccaagcgcgt gaacaacgcc agaccatgga aatccataag cgtggtggtg tggatagctc
     6181 ttacgacctc tatgttgacg ttcctgatga agaagatgtc atcctgcgga ttttggaact
     6241 gctacgtgga acttccttgg ttaacatcca catcaacgag gaaaatcgtg aggatattca
     6301 cggaatccta caaatttcat ttaaaaatgc tcaagacttg gaaagagctg agcatctcat
     6361 aacagaaaat accgactata cagtcgtcat caaataagga gaagaacatg tcaaatattt
     6421 acgatagtgc aaacgaactc agtcgcggtc tacgcggatt accagaatac aaggctgtta
     6481 aagcagctaa agatgcgatt gcagcagatg ctgaggcaag caaaatcttt acagaatatc
     6541 ttgcctttca agaggaaatt caaaaactag cacagacagg tcaaatgcca gatgcgtcct
     6601 tccaagctaa gatggaaggc tttggtaaac agattcaagg aaatagcctc ttgtcagaat
     6661 tctttaccaa gcaacaacaa ttggccattt acctttctga cattgaaaaa attgtttttg
     6721 aaccagtttc agaattgctg aaataagaaa agaactatcc taaagtcagg aatttttaag
     6781 gaatttctgg ctttttatga taaaataggt aaaagtattg caagtatgag gtccagtatg
     6841 aaactaaaaa caaacattcg ccatttacat ggtagtatcc gcgtcccagg tgacaagtct
     6901 atcagccacc gttccattat ctttggaagt ttggctgagg gtgagaccaa ggtttatgat
     6961 attctgcgag gtgaagacgt tctttcgacc atgcaggttt ttcgtgacct tggtgttgaa
     7021 attgaggata aagatggggt tattaccatt caaggtgtag gcatggctgg cttaaaagcg
     7081 ccgcaaaatg cccttaatat gggaaattct ggcacctcga ttcgcctgat ttcaggtgtc
     7141 cttgctggtg cagatttcga agtagagatg tttggagatg atagtctttc caaacgtcct
     7201 atggaccgtg tgacccttcc actgaaaaaa atgggcgtca gcatttcagg acaaactgaa
     7261 cgagacttgc ctccccttcg cttaaaaggg acgaaaaacc taagacctat tcattatgag
     7321 ttgccaattg cctctgccca agtcaagtca gccttgatgt ttgcagcctt acaagctaag
     7381 ggggagtcag ttattatcga aaaagagtac acccgtaatc atactgaaga tatgttgcaa
     7441 caatttggtg gtcatttaag tgtggacggt aagaaaatca cagtccaagg gccacaaaaa
     7501 ttgacaggac agaaggtggt cgtaccagga gatatttcca gtgcagcctt ttggttagtc
     7561 gcaggtttga ttgctccaaa ttctcgtcta gtgctgcaga atgtggggat caacgaaact
     7621 cgcaccggta ttattgatgt cattcgtgcc atgggtggaa aattggaaat aactgaaatc
     7681 gatccagtcg ctaaatctgc aaccttgatt gttgagtctt ctgacttgaa aggaacagag
     7741 atttgtggcg ctttgattcc acgtttgatt gatgaattgc ctattattgc cctacttgcg
     7801 acccaagccc aagatgtaac agttatcaag gatgctgagg agctcaaggt caaggaaaca
     7861 gaccgtattc aggttgtggc agacgcctta aatagtatgg gagcagatat tactcctacg
     7921 gcagatggga tgattatcaa aggaaaatca gctcttcacg gtgctagagt caatacgttt
     7981 ggtgaccacc gtatcggcat gatgacagct atcgcagccc tattggttgc aggtggagag
     8041 gtggagcttg accgtgcaga agccatcaat accagctatc ctagtttctt tgatgatttg
     8101 gagagcttga ttcatggcta aggtgttatt agggtttatg ggggctggaa aatcgactat
     8161 tgcaagaggc ttggacccta attaccttga tatggatgct ctgattgaga agcgcctagg
     8221 tatgtccatt gcgaattttt tcgctgaaaa gggagaagca gcctttcgtc aggtagaatc
     8281 agaagtccta gctgatttac tacaaacaga ccaagtcgtg tcaactggag gaggagtggt
     8341 tatttctcag agaaatcgtg acttactcaa gactaataca gataacatct acctgaaagc
     8401 agattttgaa accctctacc aacgtatcgc agctgataag gacaatcagc gaccgctttt
     8461 tctaaataat agcaaggaag aactagtagc tatttttcaa gaaagacagg cttggtatga
     8521 ggaagtggct agtcgggttt tggatgtgac caagctaagc ccagaggaaa ttatagagga
     8581 actaagatga aaattgctta tctaggtccc aaaggatcat tttcacacca cgttgtgcag
     8641 acagcttttc ctcatgagga attgcaggct tttgccaaca ttacagatgt catcaaggct
     8701 tatgagcaag gattagtgga ctattctgtg gtgccagttg aaaattctat tgagggtagt
     8761 gttcatgaaa ccttggacta tctttttcat caggctcaca tccaagcagt agcagaaatc
     8821 gttcagccta ttcatcagca gttgatggtg gttccaggcc atactaagat tgaaaagatt
     8881 ttttcacatc cacaggcctt ggctcaagga aagaaattca tcgatgaaca atatccagag
     8941 gctcaaatcg aggtaacagc tagtacagct tatgcggccc gttttatttc cgaacatcca
     9001 gaccagccct ttgcagcagt tgcacctaga agttctgctg aagaatatgg attggaactg
     9061 attgccgagg atattcagga aatggaagcc aatttcacac gtttctgggt tctaggagct
     9121 gaaaagccta gtattccctt gcaagcacaa actgaaaaga tgagtttggc cttgacatta
     9181 cctgacaacc ttccaggtgc actttataag gccctgtcga cctttgcttg gcgaggaatt
     9241 gacttgacaa aaattgaaag tcgtccactc aagacagcac tgggtgaata ctttttcatt
     9301 atcgatgtgg attataccga taaggacttg gtccactttg cccaaaaaga attagaagcg
     9361 attggaatcc agtataaaat tctgggtgcc tatcctattt atccaatatc agaccatgga
     9421 aaggagagaa gatgagtaaa gaaaatccct taagtcatca tgagcagttg cgttatgatt
     9481 atttgctaaa aaatattcac tatctcaatg agagagaaaa aaatgagttt gcctatttgc
     9541 aagaaaagct aactcttgct aggggaaata gtagctctag cttggaacaa gaaagagaag
     9601 agcaggttga cttaccaagc tatgcgaacc ggagtcgctc acaatccaaa tcacaagcac
     9661 tctctttccc tccaaaaaag aaaagacgga agctccgtct taaacgaatt tttatggtga
     9721 ttttttcact tttggtctgt gtggctttgg ccatggtatt catgtttttg cgtggttacc
     9781 aagatgctag cgcaaagaaa actgctgatg cccgggcagc tcaagtagaa gtctttaatg
     9841 gtcaggacac tagagatgga gttaatattt taatcatggg tactgatggt cgaatcggcc
     9901 agaacagtgt tgagacacga actgactcta ttatggtatt aaatgtcggg ggctcagata
     9961 agaaaatgaa gctagtcagt ttcatgcgtg ataatttggt ctatatagat ggttatagtc
    10021 aagtgattaa tggtagaaaa cagacagata acaagttaaa cgtagcctac gagttaggag
    10081 aacaagaggg gcaaaaaggg gcagaaatgg ttcgccaagt cttgaaagat aattttgact
    10141 tggacattaa gtactacgcc ttggttgatt tccaggcctt tgccacagcg attgacacgc
    10201 ttttccctga tggggtgacg attgatgctc aattttcaac attgaatggg cgtcccctaa
    10261 cagaagctac agtcggagat gatttatatg ctaccgagac tgagtctcca acccaaacca
    10321 ttaaagtcgg aaaacagcag atgaatggct cgaccctgct caattatgct cgtttccgtg
    10381 atgatgatga ggcggattac ggccgtacaa aaagacagca acaagtttta acagcaattt
    10441 tagagcaaat taaagatccc actaaacttt tcactggttc tgaagctctt ggaaaggttt
    10501 ttgctatgac ctcaaccaac gtaccttata ctttcctcct aacaaacggt ttatctgttt
    10561 tggatggagc aaaaaatggt attgaaaaat tgacgattcc agaactgggt gactgggtag
    10621 atgcctatga tgtttatgga ggcttgggct tgctggttga tcaaaacaaa tatcaaacca
    10681 agctcgctca aatgggcttg agataagata atactcaatg aaaatcaaag tgcaaactag
    10741 gaagctagcc gcaggctgct aaaaacacag ttttgaggtt gtagataaga ctgacgaagt
    10801 cagttaccta tacctacggc aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt
    10861 ataagacaga aaaatcaaag tttgatagct atttggatag cagtcaggct ttgatttttt
    10921 acagtctgca atagattttc accccttgtt tgtggtataa tgaaacgata cgatagaaag
    10981 aataatgaac aatatgactg atttaaaagc aattcaggct cgtagtctgg agatggctga
    11041 atattttgtg gccttttgta aagaacatga tttgctctgt tatctctgtg gcggaggcgc
    11101 tattggtgcc cttcgaaaca agggatttat tccttgggac gacgacctag acttttttat
    11161 gcctcgtaaa gattatgaga aattagcaga attatggcct cgttatgcag atgaacgtta
    11221 tttcttgtca aagagtcaca aggattttgt tgatcgtaat ctttttatta ccattcgtga
    11281 caagaaaacc acctgtatca agccttatca gcaggatttg gatttgccac atggtctggc
    11341 cttggatgtt ttgcctttgg attattatcc gaaaaatcca gctgagcgga aaaaacaggt
    11401 tcgttgggcc ttgatttatt cactcttttg tgcgcaaact attccagaaa agcatggtgc
    11461 tctcatgaaa tggggaagtt gcattttact gggtttgact ccaaaatctc tccgttatcg
    11521 catctggaaa aaagctgaga aagaaatgac taagtatgat ttggctgatt gtgatggcat
    11581 tacagaatta tgctcaggtc ctggctacat gagaaacaag tacccaatca catcttttga
    11641 agacaatctt ttcttgccat ttgaaggaac agagatgcct attccaatcg gctatgatgt
    11701 ctatctcaga actgcttttg gggattatat gacgcctcca ccagcagaca agcaggtatc
    11761 gcatcatgat gctgtcatcg ctgatatgga taagtcttat acagaataca agggagaata
    11821 tggtggctaa gaaaaaaatc ttatttttta tgtggtcttt ttctcttgga ggtggtgcag
    11881 agaagattct atcaactatt gtttcaaatc tggatccaga aaagtatgat attgatattc
    11941 ttgaaatgga gcactttgac aagggatatg aatctgttcc aaagcatgta cgcattttaa
    12001 aatcccttca agattatcgc caaaccagat ggttacgagc ttttttgtgg agaatgagaa
    12061 tttattttcc aagactgact cgtcgtttgc ttgtaaaaga tgattatgat gttgaagttt
    12121 cttttaccat tatgaatcca ccactgttgt tctctaaaag aagagaagtc aagaagatat
    12181 cttggattca tggaagtatt gaagaacttc ttaaggatag ctctaaaaga gaatcacata
    12241 gaagccagtt ggatgctgcg aatacaattg tagggatttc aaaaaagacc agcaattcta
    12301 tcaaggaagt ttatccagat tatgcttcta aattacagac aatctacaat ggatatgatt
    12361 ttcagactat tctagaaaaa tctcaagaga agatcgatat cgagattgct cctcaaagta
    12421 tctgtactat cggacggatt gaggaaaata agggttctga ccgtgtagtg gaagtgatac
    12481 gattattata ccaagaggga aaaaactatc atctctattt tatcggggct ggtgatatgg
    12541 aagaggaact gaaaaaacga gtcaaagagt atgggattga ggactatgta catttccttg
    12601 gttatcaaaa aaatccttat cagtatctat ctcagacgaa agttctcttg tctatgtcta
    12661 aacaagaagg ttttcctgga gtgtatgtgg aggccttgag tctgggactc ccttttatct
    12721 ctacggacgt tggaggggct gaggaattat cccaagaagg acgatttgga caaatcattg
    12781 agagcaatca agaggcagct caggcgatta ctaattacat gacttctgcc tcaaactttg
    12841 atgtcgatga ggctagccaa ttcattcaac aatttacaat tacaaaacaa atcgaacaag
    12901 tagaaaaact attagaggag tagcatggaa actgcattaa ttagtgtgat tgtgccagtc
    12961 tataatgtgg cgcagtacct agaaaaatcg atagcttcca ttcagaagca gacctatcaa
    13021 aatctggaaa ttattcttgt tgatgatggt gcaacagatg aaagtggtcg cttgtgtgat
    13081 tcaatcgctg aacaagatga cagggtgtca gtgcttcata aaaagaacga aggattgtcg
    13141 caagcacgaa atgatgggat gaagcaggct cacggggatt atctgatttt tattgactca
    13201 gatgattata tccatccaga aatgattcag agcttatatg agcaattagt tcaagaagat
    13261 gcggatgttt cgagctgtgg tgtcatgaat gtctatgcta atgatgaaag cccacagtca
    13321 gccaatcagg atgactattt tgtctgtgat tctcaaacat ttctaaagga atacctcata
    13381 ggtgaaaaaa tacctgggac gatttgcaat aagctaatca agagacagat tgcaactgac
    13441 ctatcctttc ctaaggggtt gatttacgaa gatgcctatt accattttga tttaatcaag
    13501 ttggccaaga agtatgtggt taatactaaa ccctattatt actatttcca tagaggggat
    13561 agtattacga ccaagcccta tgcagagaag gatttagcct atattgatat ctaccaaaag
    13621 ttttataatg aagttgtgaa aaactatcct gacttgaaag aggtcgcttt tttcagattg
    13681 gcctatgccc acttctttat tctggataag atgttgctag atgatcagta taaacagttt
    13741 gaagcctatt ctcagattca tcgtttttta aaaggccatg cctttgctat ttctaggaat
    13801 tcaattttcc gtaaggggag aagaattagt gctttggccc tattcataaa tatttcctta
    13861 taccgattct tattactgaa aaatattgaa aaatctaaaa aattacatta gaacgggggt
    13921 gaccaagtga ttgatgggaa acgattatta tttagtttga ccatagtcag ttatgccttg
    13981 acgctagtaa gtggaattgt gtatctgttt aataataata atgttagctt actttctact
    14041 ttattgttct tgttggttag tagcttgatt gcttgttgga atgatatcaa gtattactta
    14101 atccatttta ttttctattt aaccattttt gtatttctgg tatcaagacc gaccattgat
    14161 tattttaggg atggtgcttt ggatacctat catccaatag cctatcgttt tgcctttata
    14221 gttgtcatgg tttcgattct gggcttgaca acaggaggcg tcctggctcg ttacttcata
    14281 gctaggaaga aaataaaagt agcaaatata ggaaattctc taaaagaggt ttatatcaag
    14341 cggttacgct ttgtatcact aggagttttt cttctaactt atcctttcta tttcattcgg
    14401 ttatttgaac ggctcttgta tcgtttgcag acttcctact atgcctacta tgcaaatttt
    14461 gaaagtaaac tgccttattt tacctacatt ttgtctacct ttacggtcta tgcaatgtgt
    14521 atgtatctgg caaccaagcc aaagaaattg caggccacag cagtgcttgt ctcctttatt
    14581 gcagctaaca ctattcattt ggcaattggg acacgaaatc cctttatttt aagtatttta
    14641 tttgcttttg tttattactt tatgcgggag caagctgaaa aaggaaaatg gattgggttt
    14701 aaagaaaagt tagcgatttt tgtaggttct cctattctca tgttagcgat gggagtactc
    14761 aattatgtac gggataatgt ccaagtttcc catacaggtt tctgggatat cttacttgac
    14821 tttatctata aacaagggac tagttttggt gttctggctc gaggttttct atttaacagt
    14881 agcctccctt accgagattt ccgtaatttt acttttggtc ctgttcttga ttattttgca
    14941 agggggagtt tgggagccat tttcggagga aaagcctttg aacatacaac caatagtgtg
    15001 gaactagcta ttgatagtaa tagttatgcc cacaatctat cctatcttgt cttgaacaag
    15061 gaatacttga aagggcatgg tatcggaagt agttatatca tggagttgta taccgactat
    15121 ggtatgattg gagtctttct gcttagtttc ttactcggca tattatttat agccatgctg
    15181 caagtagcct atcgctcaag gacaatccta tttgctttat ccctactcat cttgaataat
    15241 ctattcttta tgccaagaag cagcttttca gaaagtttct tcaatttatt tacaatgcaa
    15301 ttctggggaa ttgttcttgt gattatattt gtagcaaaaa tgcttacaaa ggaaaactag
    15361 tatctactaa acaaaggaga aaaaaatcat gtttgaacat tattcagtag ctgatttgtt
    15421 tgcaaatctt tataaaaaac gaaaagcaaa tattttagct ctcatcgctt tatttgctct
    15481 cattgctgta ccatttacaa ttaaagcagt taggaataaa aacactgtca aagatacaac
    15541 aagttattca acttatctta tctataaaat cactcctcca gaagattcgg ccaaaacgat
    15601 tttgaatcat caaattggtg gttatagtga tttttatggg aaattgattg atggtaattt
    15661 gaatggagct tatcttttca atgatgtaga acccagtgag ttgaaaaaaa ttgccagtga
    15721 attagacacg acagaaacaa ccttgaaaaa ttcaacgaat gactattggt ggaaaaaatt
    15781 gaccgtctac tatatgattg acgatgcagg ggttggtgtg aaaattttga catcaagtaa
    15841 agatgccaat gacttgttag agaaaaaaat tgatgggttg attgagaaat ttaaacatgc
    15901 ctatgcaaat gtaaaaattg aaaaactgga aaccatcaac tctaaagaat tgaacgcaaa
    15961 tggtgaaaca gcgcttggct taaatgtgaa aaatctgatt cttcgtttag ttgttattgg
    16021 agtggtttgt gtgattttgg ttgtgatggg aaatgtgtta gtttatctct ttaatccaac
    16081 aatcaataga gtaggtgatt tttctcagta tcaaattgat tttgtaacag agattacaac
    16141 aattgctaac ctagcagatg ttttgtcata caaaaatact ggacaggaat tgaccatcgt
    16201 tagctcaaat aaagctatcc tagataaatt gaaacagagt caagaagctt taaaaggaat
    16261 gcattttgta gatttacagg atgtatcatc tcttttggaa agagatacag tccttcttgt
    16321 tgaagagtac ggagtgactc gttataagaa atttgagcaa agtcttcaaa ttctcagaaa
    16381 cttaaatcgt tctatcctcg gtgtagcaac ctttaaatta taagtattct gatgtataag
    16441 gtcttcaaaa tcggctctta gccgattttg aaggcttttt ctatctgtct gcagaaattt
    16501 tctcttggag tctcctatag aaagacccta gggaaaatct atccgtatag aggagccttc
    16561 ttttgataaa atcgtaaaaa tctagtataa tagatagaac tgaaagtatg aggttactag
    16621 caatgaaaat gaaacaaatt agtgatacaa ctttaaaaat cacgatgtct ttagaggatt
    16681 tgatggatcg tggaatggag attgctgact ttctcgttcc tcaagaaaaa acagaagagt
    16741 tcttttatgc tatcttggat gagctagaga tgcctgatag ctttctggat acaggcatgt
    16801 tgagcttccg tgtgactcca aaacctgata aggtagatgt ctttgtgacc aagtcaaaga
    16861 ttgaccaaaa tctagatttt gaagacttat cggatttgcc agatatggaa gaattggctc
    16921 aaatgtctcc agatgaattt atcaaaaccc tggaaaaaag catcgcagac aaaaccaagg
    16981 atgatatcga agccattcaa tctcttgagc aagttgaagc caaggaagaa gagcaagagc
    17041 aggctgaaca agaagctgag agtaagaaag aaccttacat ctactacatc ctttcttttg
    17101 ctaagttggc tgacttggta gtttttgcca agacagtgac ttttgaaatg gaaacttctg
    17161 aactctacaa aatgaacgag cgctattatt tgaccatttt agtggatatt gaaaatcatc
    17221 caagcccata tccagcttgg ctgttggccc gtatgcgcga gtttgcagac gatagtgata
    17281 tcagtcgctc agtcttacaa gagtatggtc aagtcttgat gagtcacgat gcagtgctca
    17341 atctgcaaaa aatcggctaa tcctttctgg aaagctattt tcagatttta agaagagcga
    17401 atcgtctgat tcgttttttc tttttatact gaaatagtga tttactataa taggaatttt
    17461 cacaaaattc tgttataatg gctatattag aaaatttcga ggagataaag atgacagtta
    17521 aaattgcttt actgggattt ggtaccgttg caagtggtgt gcctttcctc ctaaaggaaa
    17581 atggaggaaa aatcaatcaa tcagcacatt cagatatcaa agttgctaag gtattggtca
    17641 aggatgaaga tgaaaaaaat cgcttgcttg cagcagggaa tgactttaac tttgtaacca
    17701 atgtggatga tattttatca gaccaggata ttactatcgt agtggaattg atggggcgta
    17761 ttgagcctgc taaaaccttt atcactcgtg ccttggaagc tggaaaacac gttgttactg
    17821 ctaacaagga ccttttagct gtccatggcg cagaattgct agaaatcgct caagctaaca
    17881 aggtagcact ttactacgaa gcagcagttg ctggtgggat tccaattctt cgtactttag
    17941 caaattcctt ggcttctgat aaaattacgc gcgtgcttgg agtagtcaac ggaacttcca
    18001 acttcatggt gaccaagatg gtggaagaag gctggtctta cgatgatgct cttgcggaag
    18061 cacaacgtct aggatttgca gaaagcgatc cgacgaatga cgtagatggg attgatgcag
    18121 cctacaagat ggttattttg agccaatttg cctttggcat gaagatcgcc tttgatgatg
    18181 tagcccacaa gggaatccgc aatatcacac cagaagacgt agctgtagct caagagcttg
    18241 gttacgtagt gaaattggtt ggttctattg aggaaacttc ttcaggtatt gctgcagaag
    18301 tgactccaac cttcctacct aaagcgcacc cacttgctag tgtgaatggc gtaatgaacg
    18361 ctgtctttgt agaatctatc ggtattggtg agtctatgta ctacggacca ggtgcgggtc
    18421 aaaaaccaac tgcaacaagt gttgtagctg atattgtccg tatcgttcgt cgtttgaatg
    18481 atggtactat tggcaaagac ttcaacgaat atagccgtga cttggtcttg gcaaatcctg
    18541 aagatgtcaa agcaaactac tatttctcaa tcttggctct agactcaaaa ggtcaggtct
    18601 tgaagttggc tgaaatcttc aatgctcaag atatttcctt taagcaaatc cttcaagatg
    18661 gcaaagaggg tgacaaggcg cgtgtcgtta tcatcacaca caagattaat aaagcccagc
    18721 ttgaaaatgt ctcagctgaa ttgaagaagg tttcagaatt cgacctcttg aataccttca
    18781 aggtgctagg agaataagat gaagattatt gtacctgcaa ccagtgccaa tatcgggcca
    18841 ggttttgact cggtcggtgt agctgtaacc aagtatcttc aaattgaggt ctgcgaagaa
    18901 cgagatgagt ggctgattga acaccagatt ggcaaatgga ttccacatga tgagcgtaat
    18961 ctcttgctca aaatcgcttt acaaattgta ccagacttgc aaccaagacg cttgaaaatg
    19021 accagtgatg tccctttggc gcgcggtttg ggttcttcca gctcggttat cgttgctggg
    19081 attgaactag ccaaccaact gggtcaactc aacttatcag accatgaaaa attgcagtta
    19141 gcgaccaaga ttgaagggca tcctgacaat gtggctccag ccatttatgg taatctcgtt
    19201 attgcaagtt ctgttgaagg gcaagtctct gctatcgtag cagactttcc agagtgtgat
    19261 tttctagctt acattccaaa ctatgaatta cgtactcgcg acagccgtag tgtcttgcct
    19321 aaaaaattgt cttataagga agctgttgct gcaagttcta tcgccaatgt agcggttgct
    19381 gccttgttgg caggagacat ggtgaccgct gggcaagcaa tcgagggaga cctcttccat
    19441 gagcgctatc gtcaggactt ggtaagagaa tttgcgatga ttaagcaagt gaccaaagaa
    19501 aatggagcct atgcaaccta cctttctggt gctgggccga cagttatggt tctggcttct
    19561 catgacaaga tgccaacaat taaggcagaa ttggaaaagc aacctttcaa aggaaaactg
    19621 catgacttga gagttgatac ccaaggtgtc cgtgtagaag caaaataaag aatagaagat
    19681 aggatgggga aactcttgac cagagggttt catatccttt ttgtgaaaag aagtttatac
    19741 tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gctgctcaaa acagtgtttt
    19801 gaggttgtgg atagaactga cgaagtcagc tcaagacact gttttgaggt tgcagataga
    19861 actgacgaag tcagtaacat atatacggta aggtgacgct aacgtggttt gaagagattt
    19921 tcgaagagta ttagttaaaa acgtgataaa ggagaaataa agatggcaga aatttatcta
    19981 gcaggtggtt gtttttgggg cctagaggaa tatttttcac gcatttctgg agtgctagaa
    20041 accagtgttg gctacgctaa tggtcaagtc gaaacgacca attaccagtt gctcaaggaa
    20101 acagaccatg cagaaacggt ccaagtgatt tacgatgaga aggaagtgtc actcagagag
    20161 attttacttt attatttccg agttatcgat cctctatcta tcaatcaaca agggaatgac
    20221 cgtggtcgcc aatatcgaac tgggatttat tatcaggatg aagcagattt gccagctatc
    20281 tacacagtgg tgcaggagca ggaacgcatg ctgggtcgaa agattgcagt agaagtggag
    20341 caattacgcc actacattct ggctgaagac taccaccaag actatctcag gaagaatcct
    20401 tcaggttact gtcatatcga tgtgaccgat gctgataagc cattgattga tgcagcaaac
    20461 tatgaaaagc ctagtcaaga ggtgttgaag gccagtctat ctgaagagtc ttatcgtgtc
    20521 acacaagaag ctgctacaga ggctccattt accaatgcct atgaccaaac ctttgaagag
    20581 gggatttatg tagatattac gacaggtgag ccactctttt ttgccaagga taagtttgct
    20641 tcaggttgtg gttggccaag ttttagccgt ccgatttcca aagagttgat tcattattac
    20701 aaggatctga gccatggaat ggagcgaatt gaagttcgtt ctcgttcagg cagcgctcac
    20761 ttgggtcatg ttttcacaga tggaccgcgg gagttaggcg gcctccgtta ctgtatcaat
    20821 tctgcttctt tacgctttgt ggccaaggat gagatggaaa aagcaggata tggctatcta
    20881 ttgccttact taaacaaata aaacagagag ggtggggttt cccactttct tcatttctag
    20941 aatatgaata aaagggattt atgaaacacc tattatctta cttcaaaccc tacatcaagg
    21001 aatcaatttt agcccccttg ttcaagctgt tagaagctgt ttttgagctc ttggttccca
    21061 tggtgattgc tgggattgtt gaccaatctt tacctcaggg agatcaaggt catctctgga
    21121 tgcagattgg tctgctcctt atctttgcag taattggcgt tttagtggcc ttgatagctc
    21181 aattttactc agcaaaggca gcagtaggtt ttgctaagga attgacaaac gatctttatc
    21241 gtcatattct ttccttgccc aaggacagca gagaccgtct gacaacttct agtttggtca
    21301 ctcgcttgac ttcggatacc taccagattc agactggtat caatcaattc ctgcgtctct
    21361 ttttacgagc gcccattatc gtttttggtg ccatttttat ggcttatcga atctcagctg
    21421 agttgacttt ctggttctta gtcatggttg ccattttgac cattgtcatt gtagggttat
    21481 cgcgattggt caatcctctc tacagtagtc tcagaaagaa aacggaccaa ctggttcagg
    21541 aaacgcgcca gcaattgcaa gggatgcggg ttattcgtgc ttttggtcaa gaaaaacgag
    21601 agttacagat ttttcaaacc cttaaccaag tttatgctag attacaagaa aagacaggtt
    21661 tctggtctag tttattaaca cctctgacct atctgattgt caatggaact cttctcgtta
    21721 ttatctggca aggctatatt tcaattcaag gaggagtgct cagtcaaggt gctctcattg
    21781 ctcttatcaa ttacctctta cagattttgg tggaattggt caagctagcc atgttgatca
    21841 attctctcaa ccggtcctat atctcagcca agcgaatcaa ggaagtcttt gttgaggctc
    21901 cagaggatat ccattcagaa ttagaacaaa agcaagttac cagtgatcgg gttttacaag
    21961 tccaagaatt gacctttacc tatcctgatg cggcccagcc ttctctgaga gacatttcct
    22021 ttgatatgac tcaaggacaa atccttggta tcattggggg gactggttct ggtaaatcaa
    22081 gcttggtgca actcttactt ggactttatc cagtagacaa ggggaacatt gacctttatc
    22141 aaaatggacg tagtcctctt aatttggagc agtggcggtc ttggattgcc tctgtacctc
    22201 aaaaggtcga actctttaaa ggaaccattc gttccaactt gactctaggt ttcaatcaag
    22261 aagtgactga tcaagaactc tggcaggcct tggaaattgc tcaagctaag gattttgtca
    22321 gtgataagga aggactcttg gatgctctag ttgaagcagg ggggcgaaat ttctcaggtg
    22381 gacaaaaaca aaggttgtct atcgcccgag cagtcttgcg ccaagctccg tttctcatcc
    22441 tagatgatgc aacctcggca ctagatacca ttacagagtc caagctcttg aaagctatta
    22501 gagaaaattt tccaaacacg agcttaattt tgatctctca acgaacctca actttacaga
    22561 tggcggacca gattctcctc ttggaaaaag gtgagttgct agctgttggc aagcacgatg
    22621 acttgatgaa atccagccaa gtctatcgtg aaatcaatgc atcccaacat ggaaaggagg
    22681 actagaatga aacgacaaac tgtaaaccag acgctcaaac gtttagccgt agatttagca
    22741 aaccatccct tcctcctttt cctagctttt ctaggaacta ttgcccaagt tggcttatca
    22801 atttacctac ctattctgat tgggcaggtc attgaccaag tcctagtggc tggttcatca
    22861 ccaatttttt ggcagatttt tctccagatg ctcttggtgg taataggaaa tactctggta
    22921 caatgggcca atcctctcct ctataatcgt ctaatcttct cttataccag agatttacga
    22981 gagcgaatca tccataaact ccatcggtta ccgattgctt ttgcggatcg gcaaggcaat
    23041 ggagagatgg tcagtcgtgt aaccacggac atcgaacagt tggcagctgg cttgaccatg
    23101 atttttaacc aatttttcat tggtgttttg atgattttgg ttagtattct agccatgctc
    23161 caaattcatc tcctcatgac tctcttagtc ttgctgttga cgccactgtc catggtgatt
    23221 tcacgcttta ttgccaagag atcctatcat ctcttccaga agcaaacaga gacgaggggg
    23281 attcagaccc atttgattga agaatcgctt agccagcaga ctataatcca gtccttcaat
    23341 gctcaaacag agtttatcca aagattgcgt gaggctcatg acaactactc aggctattct
    23401 cagtcagcca tcttttattc ttcaacggtc aatccttcga ctcgctttgt aaatgcactc
    23461 atttatgccc ttttagctgg agtaggagct tatcgtatca tgatgggttc aaccttgacc
    23521 gtcggtcgtt tagtgacttt tttgaactat gttcagcaat acaccaagcc ctttaacgat
    23581 atttcttcag tgctagctga gttgcaaagt gctctggctt gcgtagagcg tatctatgga
    23641 gtcttagata gccctgaagt ggctgaaaca ggtaaggaag tcttgaccag tgaccaagtt
    23701 aagggagcta tttcctttaa acatgtctct tttggctacc atcctgaaaa aattttgatt
    23761 aaggacttgt ctatcgatat tccagctggt agtaaggtag ccatcgttgg tccgacaggt
    23821 gctggaaaat cgactcttat caatctcctt atgcgttttt atcccattag ctcgggagat
    23881 atcttgctgg atgggcaatc catttatgat tatacacgag tatcattgag acagcagttt
    23941 ggtatggtgc ttcaagaaac ctggctcaca caagggacca ttcatgataa tattgccttt
    24001 ggcaatcctg aagccagtcg agagcaagta attgctgctg ccaaagcagc taatgcagac
    24061 tttttcatcc aacagttgcc acagggatac gataccaagt tggaaaatgc tggagaatct
    24121 ctctctgtcg gccaagctca gctcttgacc atagcccgag tctttctggc tattccaaag
    24181 attcttatct tagacgaggc aacttcttcc attgatacac ggacagaagt gctggtacag
    24241 gatgcctttg caaaactcat gaagggccgc acaagcttca tcattgctca ccgtttgtca
    24301 accattcagg atgcggattt aattcttgtc ttagtagatg gtgatattgt tgaatatggt
    24361 aaccatcaag aactcatgga tagaaagggt aagtattacc aaatgcaaaa agctgcggct
    24421 tttagttctg aataagccat tctcttttga aagtttatgg acgaaaaaag ttgccttcga
    24481 gtgacttttt tgttacaata gctagaaaaa ttgttcactg taatactcaa tgaaaatcaa
    24541 agagcaaact aggaagctag ccgtaggttg ctcaaagcac agctttgagg ttgtagataa
    24601 gactgacgaa gtcagctcaa aaca
//
LOCUS       DAVLYV010000019        24079 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLYV010000019 DAVLYV010000000
VERSION     DAVLYV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24079
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.019"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(278..907)
                     /locus_tag="VYO70_000837"
     CDS             complement(278..907)
                     /locus_tag="VYO70_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1713101.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(952..1191)
                     /locus_tag="VYO70_000838"
     CDS             complement(952..1191)
                     /locus_tag="VYO70_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713102.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1341..1607)
                     /locus_tag="VYO70_000839"
                     /pseudo
     CDS             complement(1341..1607)
                     /locus_tag="VYO70_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1632..1943)
                     /locus_tag="VYO70_000840"
     CDS             complement(1632..1943)
                     /locus_tag="VYO70_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1713103.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(1868..2110)
                     /locus_tag="VYO70_000841"
                     /pseudo
     CDS             complement(1868..2110)
                     /locus_tag="VYO70_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2111..2743)
                     /locus_tag="VYO70_000842"
     CDS             complement(2111..2743)
                     /locus_tag="VYO70_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1713104.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(2757..3752)
                     /locus_tag="VYO70_000843"
     CDS             complement(2757..3752)
                     /locus_tag="VYO70_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1713105.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHNLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(3749..4447)
                     /locus_tag="VYO70_000844"
     CDS             complement(3749..4447)
                     /locus_tag="VYO70_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1713106.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQLKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4524..5534)
                     /locus_tag="VYO70_000845"
     CDS             complement(4524..5534)
                     /locus_tag="VYO70_000845"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1713107.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSEQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(5518..6525)
                     /locus_tag="VYO70_000846"
     CDS             complement(5518..6525)
                     /locus_tag="VYO70_000846"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1713108.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTETLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6512..7465)
                     /gene="mvaD"
                     /locus_tag="VYO70_000847"
     CDS             complement(6512..7465)
                     /gene="mvaD"
                     /locus_tag="VYO70_000847"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1713109.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7447..8325)
                     /gene="mvk"
                     /locus_tag="VYO70_000848"
     CDS             complement(7447..8325)
                     /gene="mvk"
                     /locus_tag="VYO70_000848"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1713110.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8447..9466)
                     /gene="cbpC"
                     /locus_tag="VYO70_000849"
     CDS             complement(8447..9466)
                     /gene="cbpC"
                     /locus_tag="VYO70_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1713111.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     EGHWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDPTTGIM
                     QTGWQYLGNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSS
                     GAMATGWYQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYN
                     GEWVR"
     gene            complement(9564..10253)
                     /locus_tag="VYO70_000850"
     CDS             complement(9564..10253)
                     /locus_tag="VYO70_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1713112.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTIQE"
     gene            complement(10265..11689)
                     /gene="gndA"
                     /locus_tag="VYO70_000851"
     CDS             complement(10265..11689)
                     /gene="gndA"
                     /locus_tag="VYO70_000851"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1713113.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(11765..13159)
                     /gene="mapZ"
                     /locus_tag="VYO70_000852"
     CDS             complement(11765..13159)
                     /gene="mapZ"
                     /locus_tag="VYO70_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1713114.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PFSEDILLPLPLDDEEQGLDPLLLEDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLATQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(13172..14329)
                     /locus_tag="VYO70_000853"
     CDS             complement(13172..14329)
                     /locus_tag="VYO70_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1713115.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARVVEIAKANA
                     QVAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(14414..14794)
                     /gene="rnpB"
                     /locus_tag="VYO70_000854"
     ncRNA           complement(14414..14794)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYO70_000854"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(14815..15144)
                     /gene="gpsB"
                     /locus_tag="VYO70_000855"
     CDS             complement(14815..15144)
                     /gene="gpsB"
                     /locus_tag="VYO70_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1713116.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(15214..15741)
                     /locus_tag="VYO70_000856"
     CDS             complement(15214..15741)
                     /locus_tag="VYO70_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1713117.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            15807..16403
                     /gene="recU"
                     /locus_tag="VYO70_000857"
     CDS             15807..16403
                     /gene="recU"
                     /locus_tag="VYO70_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1713118.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGKK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            16400..18559
                     /locus_tag="VYO70_000858"
     CDS             16400..18559
                     /locus_tag="VYO70_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1713119.1"
                     /translation="MNKPTILRLIKYLSISSLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTIAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(18726..24029)
                     /locus_tag="VYO70_000859"
     CDS             complement(18726..24029)
                     /locus_tag="VYO70_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1713120.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVANKEQQGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKIPGNSTWYKGNRVAAPETGSINRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPEVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSSQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQTGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEATEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDASTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
BASE COUNT         6828 a         5604 c         4388 g         7259 t
ORIGIN      
        1 ttacattata gtaaaacgaa ataagaacag gacaaatcga tcaggacagt caaatcaatt
       61 tctaacaatg ttttagaagt agaggtgtac tattatagtt tcaatctact atacaagaca
      121 aaattgaaga aatctttcca ttataaaacg catattatca aggtttttaa acacctaata
      181 atatgcgttt tttcctattt taaagacttt tatccaccta ttatacaaag ttaagtatcc
      241 atcacagttt acaattagcc ttcattacct aacccattca ccatccttca agagagtaac
      301 ctttaagaac cgaataaata aatggcaaat ttcgttcatt taatttaact gcactgttaa
      361 tttgattagc tccatctcct aaagtatgca ctcctactac acggtgacta gcatcataaa
      421 ctgtagatcc actagacccc tctaaagtat caacttggta atccaagaac ataccatcat
      481 cacttaaaac ttgtttttta tctgtataca tttgatgaat tttaaaatta tatgatagat
      541 agcctgtgat agtcacagtt attcctgtca aatttttttg actagtagga agacccaaag
      601 tccctaattt tgcaccaatg ggctcttcta gaattaataa agccaagtca tattcccttg
      661 catccttaga atttaaattt ctaaattcct tcaaataacg aacttccttt actttgatct
      721 ttccaaatag ttcttgactt ggactaacag ccggaagaac ataaatatca tccgcttctt
      781 tcccatagtc atgtctgtag tagttgtgag ccgctgtaat taacacatta tctttaataa
      841 aattcgcact tcccctccct tctacattca ttccatcggc tactccataa tacttagata
      901 aaaccatagc tgaagtcgaa tacggatact gtaaagtatt atcaatttta atcaaatcat
      961 cattaccgat aatacttctg attgcttttg gtagtatgaa ccatacgttg gtgaaatctc
     1021 agataatgaa gaatcattag actctggacc tttttctagt gtctcactta cctcatattc
     1081 ttcacctgta ctagaaataa cactcaaagc agatactgtc gataactggc tagccaataa
     1141 agtactcgca ataattgaaa tacccaattt tttataaaca gttttcttca ttattgtgtc
     1201 ctcctaatgt aattatagcg tactattcta aatttcttaa tctactatag aatcaagaaa
     1261 tctaccacct tctttaaata ccctccatta tcacataaac aggtaaactt ttcaattaat
     1321 gactgcgctt ttcaatcacg ctagaggtac ttacttgctt ctttgatact aagttcagcc
     1381 attctttccc tgtttttctc aataaagcat gttacccaag tgggattcgt tttggagtag
     1441 tctcgcagag tccagccaat ggctttattg ataaaaaatt ctgtttggtt caagttatga
     1501 aggagaatct tttccattaa ttgagtattg gtcttctctt ttcttaacaa ctggtggtca
     1561 atagcgacac gtctcagcca gatattatct gataggctcc attttatact caatgaaaat
     1621 caaagagcaa actaggaagc tagccgcaga ttgctcaaaa cactgttttg aggttgcaga
     1681 tagagctgac gtggtttgaa gagattttcg aagagtatta agattatttc ttctagttca
     1741 gggtgttcat acaccaaact ccctactact cgatctagga tatctaccgt gtcccaccag
     1801 gattttgtca cgactaactg ctctagctta ggcaaatcgg tttcctttag ataagactgc
     1861 attgctttca aatagttagc agccacatat tggtattttc taggctcctt ttcccagcaa
     1921 gtgtctgcaa aatcccaatc gataatcttt gtttttttcg cttctagtaa atatttttat
     1981 agagtttatt tctttcaggc accgcaatac ctagaaaaga aaattgatgg cgcatatagg
     2041 cttccatgga ccttgctttt ttagagtctt ttgctgcttc tagctcctca agtaaatctg
     2101 ctaaactcat ctaaaactca tcttgcccca ccaaatggtg ctgaaaggca tagacagccg
     2161 cctgggtacg atcgctgact tcaagtttgg caagaatgtt ggacacgtgg gtcttgaccg
     2221 tcttgagaga gataaagagg tcatctgcga tgcgctgatt ttcgtagccc ttggcgatga
     2281 gttggagaac atctcgctca cgcgcagtca attcttcatg aagttccata tgattgcggt
     2341 ggtattcaac cttcttgcta acctcttgct caatggccag ctcgccagca gctaccttac
     2401 tgacggcatg aagcaattca tctgcactag aagtcttgag catatagcct ttggcaccag
     2461 catctaagac tggcatgatt ttttcattgt ccaaataaga ggtcacaatc aaaatcttgg
     2521 cttcaggcca ttctttaagg attgctaagg tcgcgtcaat tccattcatc tcaggcatga
     2581 caatatccat gacaatgaca tctggacgca gttccaaggc caagtcaatc ccttgagacc
     2641 cgttggacgc ctcacccaca acttctacat cgtcttggag gtcaaagtag cttttcaagc
     2701 ccaaacggac catttcatgg tcatctacta gtaaaatttt catctttact cctttatcat
     2761 tccttatcta acaggggaat acggatatca actgccagcc cttgcttggg agctgttaat
     2821 aactgaaccg tccctgccat atcttcaacc cgctccttga tatttcgcag tccataactc
     2881 aagtcgtcta agctccctaa ctggaaacca atcccattgt ccaccacctt cagttgcaat
     2941 tcaacatctg tctgatagag gtagacatct aggcaagatg cctgggcatg gcggagcgta
     3001 ttgctaatca actcttgcag gatacggaag atatgctcct cgattttctt aggcaatttc
     3061 gtcatattct gcttgagact aaccctaaga tcactcttgt cctcaagctc ttttaagaga
     3121 atttgaatcc cttctatcaa gctcttctgc tccagttcaa ctggtcgcaa atgcaagagc
     3181 aaaacccgca aatccttctg ggctgtttct aaaatagctg tgacactctg caactgggtc
     3241 tgcatctttt ctctatccaa tttcaaagcc tgctgactga tacccgataa aatcatgtgg
     3301 gccgcaaaca actcctgact gactgtatcg tgcaaatccc gagcaattcg cttccgttcc
     3361 ttctcgatga tttcctcttc ctgagcaagg ctatgatttt cagctttttg aagagcttct
     3421 gtcaaaaggt taagtttacc tgataaggac ttgaaactgg catccaaatc tggatctgca
     3481 acctgaacca cttcttgccc tgccaataaa cgcttgagat tagcctgcat ttttcttaga
     3541 gaaagctctt cgatccctcg ccaaaacagg gctaagagac aggttatgga catgctgaaa
     3601 accaacaata aaaagacaaa tttttctgtt ttttcgacat cgtgcaaaaa gatagaccag
     3661 tcaaaatcaa gtatttccag caagttgtgg gagaaaaaaa agacaaacag gaaggaggtg
     3721 agagcaataa tgacataggc ttgttttttc atcctctaac cacctccaca tcaccaatca
     3781 tagtggtcaa gaaaatcttg acactcttgt tactcttgag atagtctttt gtttcttgat
     3841 gatagtgttc attgcggagg gctcgcttga gctggttgaa aaaagtcaaa tccccataga
     3901 gacagttaac gctgagactg acttccacat ctacaggtac gatgattttg gtcgttccta
     3961 ccatctttct gaggataatg acattgtcat gattggttaa gatgaccctc tccagatgaa
     4021 tagtgtcctt gcccatgaag cgaaagagat tgatatcatc gaattggcaa gtctggtagc
     4081 ttgaaaaatg atgaagattt ccaaaccaac gatttttctc cttcttaacc gtcacgacct
     4141 cttcaaaaac caaattggtc tgctcttttt cctggttcat catcgggtaa agaagaaaga
     4201 ggctatagat aaccgcaaca aaaatagcta gaatcacaaa aggattgagc ataacgatga
     4261 aaaagaagag aatggttgcc gctactaaaa gaagattatt tccctcttta ccagtgtagt
     4321 agcgaatcaa aagcaaaaag aggaatagta tcagcagaaa acgcgaaaaa tgctctgata
     4381 ccatcaaaat cagagctcct gtcagaagac aggcttcgat aaataaaaag attttaaatt
     4441 ttctcatagg ttcatcctct cccttctatt ttatcacaat tcaaaaaagt cacctcagtc
     4501 tgaggatgga aaaaaggcgc tggttacgcc tttttcatct gatcctttgc ttcttttaat
     4561 tttccataaa gaagatagtc tactttttgt agatctgcta tggtggcaca gttaagggaa
     4621 cacataatca agcgtaggtc tgctttccag ccttggacaa tgccaatcac ttcttcaact
     4681 gtgtaggttt caaccaattc cagaacggtt cgtgacaatc ccacagactt agcaccaaaa
     4741 accaagcact taatcatatc cagcggattc cgaacccctc cactaaccaa gagttcgacc
     4801 ttatctttcc attcttgggc attgagaagg gcctgcatgg tagactgacc ccattgattg
     4861 aggtaatcac gctggccact acgacggttt tcgatatagg caaagctggt gccaccacga
     4921 cccgataggt ccactgtacg aacaccgaat tcataggctc tttcgattgt cttggcatcc
     4981 attccaaagc ccacttcctt gaggacaata ggaacgggaa tttgctcgct ataatctgct
     5041 agatgcgatt gccagcttct aaacttcctt tctccctcgg gcatgagtaa ttcctgcatg
     5101 acattgacat gcacttgcaa tagaacagga ttcatctctt ctacagtctg aagtcctaac
     5161 tcgacaggct tgtccaatcc aatattggtt ccaaggagga gattgggatg actagacttg
     5221 acagaaaaag aatcatccgt tggatttttg agggctgcgc tataagaacc cgttacaaat
     5281 aaaataccac aggattccgc cacctgagcc agcttttgat tgatttctct tcccttatta
     5341 cttccaccag tcatggcatt gatataaaaa ggaaagtccc actttcgacc agcaaactct
     5401 gtcgaaagat cgatttcatc cagattgtaa agaggcaagg aagaatgaat cagctccacc
     5461 tcatcaaagc tattatagga acttttctgc tcaagggcat agaggatatg ctcatcctta
     5521 cgatttgtcg tcatgtccta tcctttcttg atataagagc tcaatcccca gatcggccca
     5581 acgatttttt aaggtttcgg ttgattgcgc atcaaaactc aaggcgatgc cacagtcacc
     5641 accaccagca ccactactct tggcaacggc ctgcaaatct tgactggctt ctttcaactg
     5701 tctaagcaaa ggcgtgtaaa tatctgtact caagccttct aaaagcttgc tggctacttc
     5761 tacttgctcg ataacttttt cggctttccc ctgctccaag gcttcgacca aagaaaccac
     5821 cgtttctttt gaggaactta aaaaattttg attgatattt tgcttgattt gctggaccat
     5881 gtgactcgat acagccactt ccttggtcca tcccactaag aaatcacatt ctaaagttgg
     5941 tttcacttgt gagataaaaa atccccaatc acgctccaga actgtcgcca agttttcttc
     6001 ttctaaccaa gcagccacct tctggcgatc aaatgactgg tagagaacca aatcctctgc
     6061 cacaatacag gcaaggtcgc ccatggaacc attgtctcct cgcttgagca agacagcgct
     6121 agtcagcttg aacaagagat tctgatcaac cgaaagatta tagagagcca gtaaagcctt
     6181 gacaaccaag acaacgacgc tgccactaga acctagacca aactttttcc cttctcgttc
     6241 cattttgcca cagattttta gggaaaaagg tcttaaattc tgaccgcgaa cagcgaggaa
     6301 gtctcccatc aaagcaatcg tttcttgaat caagctgtag tcaggattag gccttaagtc
     6361 cactgcgaaa tcaaacatat ctgaatagat acggtagctg tcagaaaaag caatttcagc
     6421 cctcatatag atgggaatat cctttatcaa cgctaactgc cctggctcta aaatagcata
     6481 ttcacctgcc caatagagtt ttccgcaagt tttaacagca atcatcttga ctcaaatcct
     6541 ttgtttttga cacaatcaag cgataacgct gaccgaaaat ttctgataaa tgctccaagt
     6601 ctttctcctg acagaagacc ttaacattgg gaccagcatc catggtaaag tagcaggcct
     6661 ctcctttctc acgaagctgg cgaacaaagt ccatagcctc ataagaggca tccgtcagat
     6721 aagaaaaggc tggactagca gtctttgtcg tagcatgcat agccagggca tttttctccg
     6781 ttaattctcc aatcttggca aaatcatttt ccttgagata aatcagcata tcctgatagt
     6841 ccttctcaga ctgacgaacc cagtcgtcga aagtcgtcga ggtttccaca caaagtttca
     6901 tcccgtcacg gctagagatt ggttttttct tgtcctctag caccaacata atcatagcta
     6961 gtttcaagtc tgtctctaca gggtaaattt ctccactatc cttatcccag gctcctagtg
     7021 gtccataaaa actccgagaa gaagagcctg aggcaaactt ggcttcctgc gctaactgac
     7081 ttctatccaa tccaagcttg aaataagcat tacaagcctt gaccagggcg gacaaaccac
     7141 tagaacttga ggacaggccc gctgccgtag gcatattgtt ttgagtatcg atacggacaa
     7201 agccctcacc agctggacga taacggtcaa taatcttact catcttggca tgctcgacct
     7261 cattttgtag ctgaccattg atgtaaaatt cgtcagctgt tacattggct ggtaaaggcg
     7321 acaaggtcgt ctctgtatac atattttcca aagttagaga aatactgcta gtagcaggca
     7381 ccatctcttt ttcttttttc tttccccaat atttgataat agcaatattt gcgtaggaac
     7441 gtactgttac aggctctcta tccatgtctg aacagctcct ttctcttcta atctttctgc
     7501 tagttcttgt gcgtgtgtca aattggttac caaggctatg atacaacctc ctagcccacc
     7561 accgctcatc ttggcaccca gagcaccatg gctaagagtc gtttcaacca aaaagtctgc
     7621 ctcagggcta ctgactccaa tttcttttaa atgtaaatgc gcttgactga ggatttgtcc
     7681 cagtccttca gcatcttttt gtgaaatcgc aacttctgct tgctgggtta attctcccaa
     7741 ggcatgcaaa aacggtaggg catccttgcc cttattttga accacttgga tggcttcacg
     7801 agtatgacca taaacacccg tatcggcaat caccaaatag gcggataaat ccatctcaag
     7861 ttctgtaaat cctacgttct tgataaagcg aataggttgg tcactgagac aggtcttagc
     7921 atccaaacca ctaggattca tatgggcaat catttcagct cgattgacca agatttctag
     7981 tacatcatga ggcagatcag cctgatagta gtcaaatacc gcacgaatgg ccgctatgct
     8041 gatagccgct gacgaaccca tcccccgttt ctcagggata gccgagtcaa tctcacaacg
     8101 aatgcaggct tctgtgatat ccaaatactc cagtgaggca taaaccgcca tggacaaggt
     8161 atcctcctca taaaggcgcc aaggactctc tgcaggaact accttacagg tcacctccac
     8221 ctccaaaaga ggcagggaaa tggcaggata accgtaaacg accgcatgtt cccctattaa
     8281 aattatctta ctatgtgcct gaccgacacc aacttttttt gtcatttttt ccttttacta
     8341 gacgaaaaaa cgtcttattt ttcatacaag tattaattct ttcctatcta ttttattata
     8401 ttttcacaaa aaaagcgatt gtttccattc acaatcactt ctttcattac cgaacccatt
     8461 caccattata attgacagaa tagccatcta cagtcgtatt cactgccaag gcacctgagc
     8521 tataagcata gtaccagttg ccattgacct ggaaccaacc tgtcttcatg tctccattac
     8581 ctgcatttag gtagtaccaa gttgaaccat cttgatacca accagttgcc atagctcctg
     8641 atgaacggag atagtaccat ttgttcccaa ggttttgcca acctgttttc atatcgccat
     8701 ttgggtggtc taaataatac caagtggtac cttcctgata ccagccagtg gccattgctc
     8761 ctgaggaacg gaggtagtac cacttattac ctagatattg ccaacctgtt tgcataatac
     8821 cagttgttgg atctaggtag taccatggag caggtttgct tctatcaatg tcaatagtaa
     8881 catgaaaatc ttgagtccaa cccttagcaa cttcaccaat tggtagtgga ttgtaagaat
     8941 catcgccaaa atttcctagc ttatttaaat aataccaatg accctcttca taaatccagc
     9001 ctgtctttac agcataatta ttatcaaaat aataagttct cttttcagca gatagaggat
     9061 attgttcacc gtatactttt cctgtatttt cagacgtttt tgcctctagt acttgtttat
     9121 ctgtttgttc tagcaaagca ccatcttgtc caaaatagta ccacttctcc tgaaggccaa
     9181 tttcattaac tggttggtta tcgaataaat tatcacgata acctgttcca ggaatttcta
     9241 agtattgcca accgacaacc atctctccaa tatcaccaaa atagtaagtt ttaccgtcta
     9301 ttttatgcca atagattgct ttatgatcat cttttacata atattttcta ttatccttat
     9361 caacaaatcg gccacctgtg gtattcgcaa ataccgtatt tgtagctagc aaaccaaaga
     9421 aaaagactgc tagtagaact tgcatcattt ttttcaaaag tttcatctat atatcctcct
     9481 aaaacgattg tagcaaagac ccgactgcat gtcaatatat aaaaactcct caaaaaagca
     9541 gttacacaac tgcaactgct tatctattct tgaatggtat atccaacacc acgcacggtt
     9601 ttaatgtagc ttttttggcc tttaacatca agcttgctcc gtagatagcg gatatagaca
     9661 tctacgatat ttgtctcggt cgcactttca tacttccaaa cactttccaa caattgctca
     9721 cgagtcaata ctttcttgct tcccataagt gtcgccaaaa ggtcatactc acggcgggtc
     9781 agagcaatca tctcttcacc acgataaacc gtgtgatgtt caacatctat cctaagattg
     9841 cggtaggtcc ttggaacttt catcagactg cagtgttgat caatgaagtc ccgacctcgg
     9901 aagatcgccg aaatacgcgc taccagattt tcgataagga ctggcttata gatgtatgaa
     9961 actgcaaaac gctgaacaac ttccagctct tcttgcaagt cttcccaatg atctaaaatc
    10021 atgatgactg aggcaggttt agttcggctc aatttttctg caaaatcctg agccatcata
    10081 tctcccagat taacgttcaa taaaatcaaa tcatagtctg tctgaagagc catggagagg
    10141 gctttttgcc cctcctctac cagatcaacc cgatactgct ctttctggag ttccaaactt
    10201 aaaaaatgag ctagatttcg ttctttctca agtaataaaa tccgtttccc catggctgac
    10261 ctacttattt ttcgtcatac caagagtagt ggaaggttcc ttctttgtct ttacgttggt
    10321 aagtgtgggc accaaagtag tcacgttgtg cttggatcaa gttagctgga aggtcagctg
    10381 aacggtagct atcaaagtaa gtaatagctg ctgagaaagt tggcactggc acaccagctt
    10441 gaaccgcaag agctacgata tcacgcactg cttgttgata cttagcagta acatccaaga
    10501 agtattcatc caaaagaagg ttggcaaggt ctgcatcacg gttgtaagca tctgtaatct
    10561 tttgcaagaa acgagaacgg atgatacagc catcacgcca gatagatgcg atatctgcaa
    10621 atggcaagtt ccagttgttt tctttagagg ctacacgcaa ttgagcaaat ccttgtgcgt
    10681 atgaaatgat ttttgagaag taaagggctt gacggatttt ttcaatcaat tcagccttgt
    10741 ctccttcaaa gttgaaggcg gctggttttg gaagcacctt gctagcatgt acacgttctt
    10801 ctttgtaagt tgaaatgtag cgtgcaaaca ctgactcagt aatcagtgac aatggtacac
    10861 caaggtcaag agatgattgg ctagtccatt taccagttcc cttgttacct gcagcatcaa
    10921 ggatgtagtc tacgattggt ccatcttggc cttcatcgtc tttacggctc aagatatcag
    10981 ctgtgatttc aatcaagtag ctatccaatt cacccttgtt ccactcagta aagatttcag
    11041 ccatatcctc tgcagaaagg cctagcaagt gttgcatcaa gtcatagctt tctgcgatca
    11101 attgcatatc accgtactcg ataccgttgt gaaccatttt cacatagtga ccagctccat
    11161 caggaccgat gtaagtcaca catggtttgc catcttctgg tgctttagct gagatttctt
    11221 caagaacatc cgcaaccaat tcgtaggctt ctttttgtcc accaggcatg atagaaggac
    11281 cttcaagggc acctttttca ccaccagaaa ccccagtacc gataaagttg ataccagagt
    11341 ttgccaattc ttcattacga cggatggtat ctttgtagaa agtatttcct ccgtcaatca
    11401 agatatcacc cttgtcaagg tgtggaagaa gggcttggat agtagcatct gtaccaggtc
    11461 cagcttgaac catcagcatg atacgacgag gtttttcgat tgagtttaca aaactttcaa
    11521 cgtcatagct tggtacaaag ttcttttcag gatggcaagc aatcacatct tccgtttttt
    11581 ctttactacg gttgtagata gcaactgtgt aaccacgaga ttcaatatta agggcaaggt
    11641 tacgacccat tacggccata cctacgacac caaagttagc ttttgtcatt tgacactcct
    11701 cttgtttcat ttgttttatt ttatcatttt tacttttgaa aagaaagaat tttgtaacga
    11761 ctgcttagta gtccaagtca tccgcatgac cagcgccatt tccgacaaag tagcctgtct
    11821 tacagttaag ggtaaagaga taggttccat ctggcttata gaggttgtaa taaccattgc
    11881 cgttaacgat attgacacgt tcaaggatat attggtctcc agtgatatag ccacgtgaac
    11941 gtgaagtcgc tagaatttgt tccaagacac catccgcaaa atcccaggca gagttattac
    12001 tatcatcaat agcagactga ttataggcaa cacgactggc acttctttga acagcaaccc
    12061 ctgcactcga gagacccata ttgacttcac tgcgactact tctagtctca tttgaactac
    12121 ttggttttgg ctcactagtc gtatttgaac ttgcttgact tgaacttgag ctgctagttt
    12181 gacttgaact tgagctagaa gtacttgttt gctggctctt accaagactg atagccttat
    12241 ctagcacttt atcaagctcc gtatttccag ttttaatatc cgtaaattta gcatccgatt
    12301 tggctttggc attggtatcc aacacaccat ccacaatagc tggtttctca aattgagcat
    12361 tgacatcttg aatagccttg atttgcgttg ctagactatc atatttagat ttggcaagcg
    12421 tatgttcacg actaccttcc agcttatcaa gtaaagtctt gagttgactc agtttatcaa
    12481 actggctatt tttcaaagcc gttttattgc tatctgtgta aaaggcgtca taaagtgtat
    12541 taaaatcatc cacatccgat tgattggctg tagttgattg agaagtttca atttccttag
    12601 tcgaacgagc cacttgacgg tagacataat aagcactgac acaaataatt actgatacaa
    12661 gcgccaaaac ggtcaaaata aagccttttt tacttttctt ttctgagtcc tcttgatcca
    12721 gacgagaaag gttttgctcc tcttccactt cttcagtcat ttctgttgga ttttcatcct
    12781 ctagcaatag aggatccaag ccttgctcct catcgtccag aggcaagggt agtaagatgt
    12841 cttcagaaaa tggaacttcc tccgaagaag cttctgactt ctctacagcc tcacgcatct
    12901 cttgaattaa atcatccaga ctctgagttt caacgaactc ctcttttttg tattgacgag
    12961 tcgcaaactt atccgcctca atttcatctc tgtgttgctt aacatacttg tccaaaatgg
    13021 aatcctcagg caagactcct gcttccactt cttcattttt acgaatagct tgaccaactg
    13081 ttagctcttt tgcttcatca aaatcaaatt gcggttcttg accttctttt ttatgacgat
    13141 ttcgtctttt tttactcatg agtatccctt tctattttac ctcttgccgt ttgacacgct
    13201 gaccaaaata ttgatataga tccactttca aggttccgtt gtataactta cgcttcttat
    13261 ctgcttggct accatacttg ctttcaaaag cttcatcact agtcaggata aatttgctcc
    13321 aagttttcag cggtgcaaat acttgcccca tctcagcata gagcttagtc acccctgcat
    13381 catctgacaa acgttcacca taaggcggat tggaaatgat tactccattg attttatcgg
    13441 aacgtaaatc ctgcacgcgc atctgcttaa aagtaatgtc tcctgcaaca ccagctacct
    13501 gagcattggc cttagcaatt tccaccacgc gagcatcaat atcacagccc atgatatcca
    13561 gctcaagctc acggtctact tttttagccg cttctgtgcg cacttcttga atcaagcgat
    13621 cgctgatcca gttccattcc tcaaatgcaa aagagcgacg aagacctggc gccatctttc
    13681 tagcaatcat aactgcctca atacagaaag tccccgaacc acaggtcgga tcaatcaaag
    13741 gcttgtctgg ataccagtta gaaagttgta aaatggctgc tgccatattt tccttgatag
    13801 gagcgccacc tttttcggta cgataaccac gtttaaagag gctagacccg gtcgtatcaa
    13861 tcatgacagt tgccacatct ttgagaatag agacctcaat cttaaactct gggccattct
    13921 ccatcagagg aaccccttct gggcgagcat agtgtttctg caatttcttg acaacagctt
    13981 tcttagaaat agcctgaaca ctgggctcat tgtgaagttt ggacttaaca catttagctt
    14041 ttgaaatcgg gaaccgagct ccaagtggta aataattttc ccaatccaaa gcgaaaactc
    14101 cctgaaatag ctcttcaaaa gtcttagctg ggaacgttcc tacgataatt ttgatacgat
    14161 ctgctgcccg aagccaaagg ttggtttcga taatagctct cacgtctcct tgaaaacgaa
    14221 cacgtccatt ttcaacctga caatcgtagc ccaactctcg cacttcacga ccaacgacag
    14281 cctcaagccc tgctgccaca gttgcaatta aattaaattc ttttttcatg ttacagtctt
    14341 gcaagacctt tctatatgtc cactttaaaa aatgaggttg agaaaatttc tcagccccaa
    14401 cctatatgca attttctata agccatgttt tgttccagaa gtgactagga ccacttcctt
    14461 cgataatcat ctgtctacca ctaacagctc tagctgatag cagtcagaat actacgttcg
    14521 tttccgcgta ctccatgccc cgaccaaaat ttgggttgct agcttgaggg gtttaccgcg
    14581 ttccactctc tctgtttcca gaaagactcc gtcactgtgg cactttcaag cctactctgg
    14641 cctatccaag gacttagcca tttcaactgc cgtaacgatt tctcgtccct aggcttatgg
    14701 tttcgcctag cacaaacact acaggcatca cagcctgtgc tagcatggac tttcctcatg
    14761 agaagcaatt ctcctcacgc gattatccaa aaattgcaca tctcaaataa ctacttaaaa
    14821 atctgagtta tctaaaattt gtttaccaaa aacttctttt tccaatctat tcaggcgttt
    14881 caaaatatca aaattcgtca tagaacttgt aattgccgct tcaaggggtt ctgcttgaac
    14941 tggtgaaggt ttcggtttac gagttaattc ttccttcaaa tccgcaattt cctgacgaag
    15001 tgacttgacc aaggcagcat aggtttcata gtccttgatg acatcgtcta aaaactcgtc
    15061 aacttctact ttattatagc cacggacttc acgtccaaac tcttgttcaa aaatatcttt
    15121 cgctgaaaaa ataatacttg ccatgtctct ctccattctc gcttgctagt attattatat
    15181 aaaaaaaggc aaacaaaaat caaggtcaaa gcttcaattt tcggaaaaat tttcagcaag
    15241 ttcatttaat tgatcaaatg ttaatctctt tataaaatag tcctcttgat ttttcatctt
    15301 ttggtaaaaa taagctagtt ttgtttcatt ttcttcatca taaaaaagat aggaactagt
    15361 cgtgttttcc agcaaaaact gctggtaatc tcttaactgc cccttgtgtt catagcgagg
    15421 ataggcatat ttgacaaagt ctacctgctt aaagcgactc agtttcatct gattgccttc
    15481 attccaattt tccccatggg tttcaaaagc aaaaatggtg gccaactgga aaccgtattc
    15541 agttttcatc tcctgagcaa cctctaaaac ccaatattca aaccccaaac tccctgtaaa
    15601 cacaagccaa gacaccccat ctgctgccat agcctctaaa tctttacgta tcgctttttt
    15661 aatcaattta agacgaggat ccttatcaga aaataaaccc aaatcaaaag cagaatagcc
    15721 taaaaccaaa gctgtagcca tttttaaccc tttctgtgca aattatggta taataaagtg
    15781 atgttattgt accaataagg agaattatgg tcaactatcc acataaagtt tcatcacaaa
    15841 aaagacaaac atctctttct caacccaaaa atttcgcaaa tcgaggaatg tcttttgaaa
    15901 agatgatcaa tgctaccaac gactactatt tgtctcaggg cttggctgtt atacataaga
    15961 aaccaactcc tattcaaatc gtacaagtgg actatccaca acgaagtcgt gccaagattg
    16021 ttgaagccta ttttcgacaa gcttcaacga cggactattc tggcgtttat aatggatatt
    16081 acatcgactt tgaagtcaag gaaacaaaac aaaaacgtgc gattccgatg aaaaattttc
    16141 atccacatca gattcagcat atggaacaag tccttgccca acaaggaatc tgctttgtcc
    16201 ttcttcactt ttcttctcag caagaaacct acttattgcc ggcattcgat ttgattcgct
    16261 tctatcatca agataaggga aaaaaatcaa tgccacttga atatattcga gaatatggat
    16321 atgaaatcaa ggctggtgcc ttccctcaaa ttccttatct caatgttatc aaagaacatt
    16381 tattaggtgg taaaacaaga tgaacaaacc aacgattctg cgcctaatca agtatctgag
    16441 cattagctcc ttaagcttgg ttatcgcagc cattgtctta ggcggaggag tttttttcta
    16501 ctacgttagc aaggctccta gcctatccga gagtaaacta gttgcaacaa cttctagtaa
    16561 aatctacgac aataaaaatc aactcattgc tgacttgggt tctgaacgcc gcgtcaatgc
    16621 ccaagctaat gatattccca cagatttggt taaggcaatc gtttctatcg aagaccatcg
    16681 cttcttcgac cacaggggga ttgataccat ccgtatcctg ggagctttct tgcgcaatct
    16741 gcaaagcaat tccctccaag gtggatcaac tctcacccaa cagttgatta agttgactta
    16801 cttttcaact tcgacttccg accagactat ttctcgtaag gctcaggaag cttggttagc
    16861 gattcagtta gaacaaaaag caaccaagca agaaatcttg acctactata taaataaggt
    16921 ctacatgtct aatgggaact atggaatgca gacagcagct caaaactact atggtaaaga
    16981 cctcaataat ttaagtttac ctcagttagc cttgctggct ggaatgcctc aggcaccaaa
    17041 ccaatatgac ccctattcac atccagaagc agcccaagac cgccgaaact tggtcttatc
    17101 tgaaatgaaa aatcaaggct acatctctgc tgaacagtat gagaaagcag tcaatacacc
    17161 aattactgat ggactacaaa gtctcaaatc agcaagtaat taccctgctt acatggataa
    17221 ttacctcaag gaagtcatca atcaagttga agaagaaaca ggctataacc tgctcacaac
    17281 tgggatggat gtctacacaa atgtagacca agaagctcaa aaacatttgt gggatattta
    17341 caatacagac gaatacgttg cctatccaga cgatgaattg caagtcgctt ctaccattgt
    17401 tgatgtttct aacggtaaag tcattgccca gctaggagca cgccatcagt caagtaatgt
    17461 ttccttcgga attaaccaag cagtagaaac aaaccgcgac tggggatcaa ctatgaaacc
    17521 gatcacagac tatgctcctg ccttggagta cggtgtctac gattcaactg ctactatcgt
    17581 tcacgatgaa ccctataact accctgggac aaatacccct gtttataact gggatagggg
    17641 ctactttggc aacatcacct tgcaatacgc cctgcaacaa tcgcgaaacg tcccagccgt
    17701 ggaaactcta aacaaggtcg gactcaaccg cgccaagact ttcctaaatg gtctcggaat
    17761 cgactaccca agtattcact actcaaatgc catttcaagt aacacaaccg aatcagacaa
    17821 aaaatatgga gcaagtagtg aaaagatggc tgctgcttac gctgcctttg caaatggtgg
    17881 aacttactat aaaccaatgt atatccataa agtcgtcttt agtgatggga gtgaaaaaga
    17941 gttctctaat gtcggaactc gtgccatgaa ggaaacgaca gcctatatga tgaccgacat
    18001 gatgaaaaca gtcttgagtt atggaactgg acgaaatgcc tatcttgctt ggctccctca
    18061 ggctggtaaa acaggaacct ctaactatac agacgaggaa attgaaaacc acatcaagac
    18121 ctctcaattt gtagcacctg atgaactatt tgctggctat acgcgtaaat attcaatggc
    18181 tgtatggaca ggctattcta accgtctgac accacttgta ggcaatggcc ttacgatcgc
    18241 tgccaaagtt taccgctcta tgatgaccta cctgtctgaa ggaagcaatc cagaggattg
    18301 gaatatacca gaggggctct acagaaatgg agaattcgta tttaaaaatg gtgctcgttc
    18361 tacgtggagc tcacctgctc cacaacaacc cccatcaact gaaagttcaa gctcatcatc
    18421 agatagttca acttcacagt ctagctcaac cactccaagc acaaataata gtacgactac
    18481 caatcctaac aataatacgc aacaatcaaa tacaacccct gatcaacaaa atcagaatcc
    18541 tcaaccagca caaccataac atttatcacc cagatttttc tgggtgattt tgtcatagca
    18601 ctattgtatt tatgtgctta acaaaagcct gagaagacat cttctcaggc tttttctgta
    18661 ctataaacct cattggaaaa gatagagact gcttccgtaa tcttgttaag aggttttact
    18721 aaatactagt ctttcttcgt ttttactaca aagagagcag atagggctag actaacacct
    18781 gctaggaaga gggctgtgtc agattgactc tcacctgttg ctggcaagat ttgttcaggt
    18841 gtttctggtc tagctatttc ttgcttataa tctggcgtct taccttgatc ggaagctaaa
    18901 gagctaagtt taaactcagg tttctctaca gttgaagcat ctggtttcgt agcagaatct
    18961 tttaattggt tgagataatc tgcaaagtat tccaccattc tttccgtcaa gagatggtta
    19021 tccgtcgcat atttcatgct tgcctgaacg ctagctactt cctcttgatt gtctttgtct
    19081 gttaatttct gagccttagc caaagccgct tcatagcctg acaagtcaag aggtgtttct
    19141 gctacctgtg gacgagtaaa gatgagttcc gctgcagatt ggtatttatc gccaccatct
    19201 ccgtaagtct tagtacctgt aaggacaatt ttcttagcct taattgtctt accaaaatca
    19261 atgtcttttg gcttattgtt atctggccaa tcagttgcag taaaggtatg ctccttgcca
    19321 gactcatctg tcacaacaag tttcacatca cgcaagttac catttgaacc tgatccacgt
    19381 ggaacataac gaagtccagt gatttcagtt gcttctttca agaccatggt tgcaggcttg
    19441 cctacatctc ctccgcccca tgatgtatgc cataaactag ataagtttcc atcaaaggca
    19501 tttgcaagac cttcttgagc ctgagcagga gctgtaagac ttgcaaagtc atctgctacc
    19561 aaagccgttt tcttctgaac caaagcattc ttcaaggctt caatcttagc aatctctgca
    19621 cgcgcttctt ccacactgat atcatcatcg gcctgactga ggttaaagac cgcctctttc
    19681 aaagcatcca tagactcttt ggtgtagtta gtcatggcaa ccgttggcaa gtagttcttc
    19741 agagcatttt ctgtcaacat cttacctgtt agggtaattt cttcgatttg aagattatcc
    19801 atcatgaagt cgttataacc acggaagttg gcatttccac cagaatcacc acgagtatta
    19861 cttgcatttc cagttgagta gatacctacc caagtatccc ctgtttctgc acctgtcacg
    19921 aggaaggttg ccttcttggc tttcttagaa tctgtccaag tatttggcaa ttcatgcatt
    19981 tccaagttgc ttgcttgagt accacgacga cctgactgga attctccctt accgactaca
    20041 aaagcatagg tattgtctga acctgcttcg tattcaaagg ttacacggta ggtcttacct
    20101 gcttcaaaac ggaagttttg tgggatggtt tggtaaacca agttacgacg gctcactagt
    20161 ccatttgtct tgagtgacca atttccttcg ataacatcat cgactttctt accattccaa
    20221 ccacgttgtg tatatggatc gtgtttttca gacaagtgag tgcggttgtc ttcgacacct
    20281 tcgacaccac ccactacaaa tgggaagata ccctgagcaa cattttcaaa gtcttgcttg
    20341 aaggtgcctt tacctgtatc atgcttgtct ccgtacatgc ttgaattgtt ttcaaaggta
    20401 cgaatttcat caaagtaagt tgcttcatca ccagcttcac gactcaatgt cagagtaaca
    20461 tttgatacgt ccgatccagt tgtaaagaag gcgtacatgt tttggaagta acttgtatcg
    20521 tcaactgtag cattgttacg acgtgtattg tgggcatagg cttttacata gttgagggcg
    20581 agagacttat tggtataagt agtcacttct ttttcaccag tattcacagt gatacttgcc
    20641 ttggcattac tacggttatc gacaccgaca taaacggcat acttggtatt tggtttcaag
    20701 ccagttaatt tctgagtgag actaactttt tctttgtttc cttgaatacg aagcatatcg
    20761 tttgcccctt gagacttgac aatttctgcc ttagaagcat cgcctgaaat ggtccaatgt
    20821 ttcaaggtac cgctattaaa tccttggtca tagatgtgca tgccttcact ccatgacatt
    20881 tcaggattgg tttgtttcga acgatagaga acgtatggtt gatttgctag aagatctagg
    20941 gtaattttac catcttttac agttagttcc tgctcttctg tcttaccttg gtcagttagc
    21001 ttgtaaaggt aaaccttgct ctttgcccaa tcgcttggaa gggtccaagt tgttgcacca
    21061 gtctgcgtat tgaagtagta catcttttcc ttatcagtag aaagtttctt accattcgca
    21121 tcccagttcc aaggagtcaa gtaagctgaa ccatcttgga tgacacgtcc gttgagagtt
    21181 actgtacgtt cgcgatattg tgagctattg acatcatttg acttacgagt tacaactact
    21241 ttattattgt cagcatctac caattccact cgcatttctg gagtccattt ataggtgcta
    21301 ccgttatcgg tcatagtcac cggtgtacca ttttcccatt tacttacagt gaagtgttgg
    21361 aagtacttag tcatgacgtc atgggcaaat aagttggtta catagccatt gtagtcactt
    21421 cttccttgcc agccttcaaa gtctttcatg ctgtagccac ctagcagtgg atagttggct
    21481 gcaccaccat aacttctgta gtcccctacc caagcatctt tttggtggtt acggataaag
    21541 cgggtgatgg cactgttgat acctttattg gtgtagccac cgtaggtcaa gtcagctgcc
    21601 cagtgatgga aggtagagtc gtactcacca ccatggcccc actcgatcgc aaagcgccag
    21661 ccttgtttgt taatttcttt agcaagaacg tgggtagccc aggcaccgtt atcacctgat
    21721 tgaccattac cccaaacgtc cacatagata aagtcgagac cgtcaccaag ttttttcttc
    21781 aaatcttccc aacgtgccaa acgaccatga gctaggtcat aggcagcatc aatgttgata
    21841 ccttgatcta gccagttcca accatagcta tagcttccat ctggattctt acggagaatt
    21901 ttttcattga agtatttaga ctcaggataa gtttctgaag cgttaacgtg gatacctaga
    21961 tgagctccat atttcttagc cttctcaatt agggtcttga agtcttcgac accaccgata
    22021 cgcttaccaa tatcagcata gttcaagtga ccagagtcgt gaccttcact accatatcct
    22081 ttaaggagaa caccttgccc aagaccatct gtgtggagat tgattttctt gataccatcc
    22141 aaggtcataa ggaatgggtt ttgtgcttga gaaccaaagt tcatcgcgat acggtaagct
    22201 gtgatatcct taactttttc ccaaccttga gggttgttca taatgctacg ataagcaatg
    22261 gcaccatctt gccaatcaac tttcttgtct gcattggcat cttcagtgat aacaacctta
    22321 gcacttggaa gttccttcgt gtattctggg aaaacaatgc ccttataagc tttttcccat
    22381 tgccattcag agctgtggat tcctacatag ttggcatttc cgactgtttc tttataagct
    22441 gtcaaacgag tccagtcatt cgaaccacca ccataactgt tttgagagtt actccaaaca
    22501 ccagcagcaa gcttatctgt agaaacaaat ccatacatgt aacccttggc taggtctttc
    22561 attggattgg ttacatcgat atgatcatct ccgctgacat gcgtattgtt tgacatggtt
    22621 gccccatcaa acttagcacc agtttgatca ctagaaacag agactaaagc attgccgagg
    22681 aaactaatag aagaaagtag ttttctttcg tcatcaatct tttgacctgg agtgacttga
    22741 ttgtggttga caatcttggt cacatcaaag tgcaattgat tgtccacaac ttgcaagcgt
    22801 actgtcattt ccgcattgat taagtgagca tcatcgcgaa tcttcatcaa gtactctgct
    22861 gttgtctcat tgattttctt ataagtgact tcaggggtga ttcggtggtt attgataaag
    22921 acttggttga actgttgcac ctgtcctggc aaagtatgcc cgttcaagct gtattccttg
    22981 acacgaggga aggcttggtc aatcactgct ttgaggacct tagactgaat cgtgtcataa
    23041 gtcaccttgc tatcatcaac ttcaggacct gtttcttttt cagcaggggt atcctctgtt
    23101 tttaccccct cttggttatc cgttttaacg ctaacaactg ttcgctcatc gtcataagag
    23161 cccgccttga gaagaatctt cttctcattt ttaagatggt cattgaccgc agctggtaga
    23221 gtcactgtgt caaagagatt gacatcgtta ttgctggcat ttagctgacc gtctgacttg
    23281 agagtgatag aaagacggtt tattgatcct gtttcaggag cagcaacacg gttgccttta
    23341 taccatgtgc tattacctgg aattttgtat tcccagaacc agccatcctt gtcataaccg
    23401 acaaaaacat tattattggt atctttaaat ttcaggaaga caccaaagcg tgatttgccc
    23461 ttttcagaat cttctttgaa ggttaaatca acagttgcat ttccattggc atcaacggtc
    23521 aagcccttct tttcaaacag ggctggtttg cctgcgttat cattttgagc agttgaggat
    23581 agttggttgt agcggacacc tttttcttct cggatagtga ctgttccctg ttgctcttta
    23641 ttcgctaccg tttgccattc aggggttacc gtcttaggtg tttcaggttt agcagctgca
    23701 ggtttatcct cttttggttt ttcttcagct ggtttttctt tttcaagtgc taatagttct
    23761 tcttctgtct taggtccatc tgtaacttct ggagaacctt ggtcttctcc caccttaggc
    23821 gcttcaaaat cacgcccatc attctctttt gctgttgcaa gagcagtagc taaatcagct
    23881 ggtaagttcg ccgtggaacc agactgcacg ctatctgcaa gaaccggact tgtcccaaag
    23941 aatgcagctc caatcataac agaagcaaca cctaatgaaa atttccgaat actatattta
    24001 caacgttttt caaataatcc tttattcata aacttctaaa tcctttcatt tttgagcaag
    24061 cgtttacatt ttcttttta
//
LOCUS       DAVLYV010000020        24060 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLYV010000020 DAVLYV010000000
VERSION     DAVLYV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24060
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.020"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(197..355)
                     /locus_tag="VYO70_000860"
     CDS             complement(197..355)
                     /locus_tag="VYO70_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713121.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(365..1675)
                     /gene="obgE"
                     /locus_tag="VYO70_000861"
     CDS             complement(365..1675)
                     /gene="obgE"
                     /locus_tag="VYO70_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1713122.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVLVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLAENYDEFEELPAIFPISGLTKQGLVTLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            complement(1739..1873)
                     /locus_tag="VYO70_000862"
     CDS             complement(1739..1873)
                     /locus_tag="VYO70_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1713123.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            complement(1932..2108)
                     /locus_tag="VYO70_000863"
     CDS             complement(1932..2108)
                     /locus_tag="VYO70_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1713124.1"
                     /translation="MRSGRDKKIAGVCAGVAYYLDMDPTIVRVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            complement(2139..3464)
                     /locus_tag="VYO70_000864"
     CDS             complement(2139..3464)
                     /locus_tag="VYO70_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1713125.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            complement(3480..4523)
                     /gene="cpoA"
                     /locus_tag="VYO70_000865"
     CDS             complement(3480..4523)
                     /gene="cpoA"
                     /locus_tag="VYO70_000865"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1713126.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAALKDLKEKAKNISREYSEENLLQIW
                     LDFYEKQAALGRK"
     gene            complement(4638..4967)
                     /locus_tag="VYO70_000866"
     CDS             complement(4638..4967)
                     /locus_tag="VYO70_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1713127.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            complement(4981..6090)
                     /gene="rpoD"
                     /locus_tag="VYO70_000867"
     CDS             complement(4981..6090)
                     /gene="rpoD"
                     /locus_tag="VYO70_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1713128.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            complement(6093..7853)
                     /gene="dnaG"
                     /locus_tag="VYO70_000868"
     CDS             complement(6093..7853)
                     /gene="dnaG"
                     /locus_tag="VYO70_000868"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1713129.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQDLYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            complement(7943..8701)
                     /locus_tag="VYO70_000869"
     CDS             complement(7943..8701)
                     /locus_tag="VYO70_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713130.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            complement(8698..9564)
                     /locus_tag="VYO70_000870"
     CDS             complement(8698..9564)
                     /locus_tag="VYO70_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713131.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            complement(9633..10637)
                     /locus_tag="VYO70_000871"
     CDS             complement(9633..10637)
                     /locus_tag="VYO70_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1713132.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEVQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     misc_binding    complement(10750..10967)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11050..13746)
                     /gene="ppc"
                     /locus_tag="VYO70_000872"
     CDS             complement(11050..13746)
                     /gene="ppc"
                     /locus_tag="VYO70_000872"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1713133.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     gene            complement(13766..14995)
                     /locus_tag="VYO70_000873"
     CDS             complement(13766..14995)
                     /locus_tag="VYO70_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1713134.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGLSVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            complement(15123..15452)
                     /locus_tag="VYO70_000874"
                     /pseudo
     CDS             complement(15123..15452)
                     /locus_tag="VYO70_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(15557..15805)
                     /locus_tag="VYO70_000875"
                     /pseudo
     CDS             complement(15557..15805)
                     /locus_tag="VYO70_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335280.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
     gene            complement(16086..16673)
                     /locus_tag="VYO70_000876"
     CDS             complement(16086..16673)
                     /locus_tag="VYO70_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV1713135.1"
                     /translation="MGGNPPIKNYTIVNKIVSSRKIERIVIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNVNSDYQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            complement(16778..18571)
                     /locus_tag="VYO70_000877"
     CDS             complement(16778..18571)
                     /locus_tag="VYO70_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1713136.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSILASQ"
     gene            complement(18738..18890)
                     /locus_tag="VYO70_000878"
     CDS             complement(18738..18890)
                     /locus_tag="VYO70_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713137.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            complement(19021..19224)
                     /locus_tag="VYO70_000879"
     CDS             complement(19021..19224)
                     /locus_tag="VYO70_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713138.1"
                     /translation="MYIRLKNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSF
                     LKQLAKLYQVTTDYILEMDKFSC"
     gene            19726..20921
                     /locus_tag="VYO70_000880"
                     /pseudo
     CDS             19726..20921
                     /locus_tag="VYO70_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(21300..22085)
                     /locus_tag="VYO70_000881"
                     /pseudo
     CDS             complement(21300..22085)
                     /locus_tag="VYO70_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(22087..22906)
                     /locus_tag="VYO70_000882"
                     /pseudo
     CDS             complement(22087..22906)
                     /locus_tag="VYO70_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            complement(22908..23900)
                     /locus_tag="VYO70_000883"
     CDS             complement(22908..23900)
                     /locus_tag="VYO70_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1713139.1"
                     /translation="MKMITVEHLQKNFVKTIKEPGLKGAFRSFFHPKRQVFEAVKDLS
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQENRQGYVKDIGVV
                     FGQRTQLWWDLALQETYSVLKEIYDVPDAVFQKRMDFLNEVLDLKEFIQDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLG
                     DIEQLCDRIFMIDKGREIFDGTVNQLKKTFGKMKTLSFELHPGQDYIVSHFEGLSDIY
                     VTRQELSLDIQYDSSQYQTADIIQQTLSDFTIRDLKMTDANIEDIIRRFYRKEL"
BASE COUNT         7389 a         5186 c         3975 g         7510 t
ORIGIN      
        1 gactcgccga gaactttgac atcagatata ctataacaaa ttgaaataag aatcaaacaa
       61 tttaattttg aaattcaaat caatttataa caatatttta gaagatatgc tgtgctattc
      121 cagattcagt ttgctataca tgcctgtcgt accacaaaat cccgtgtctg atgaggcacg
      181 ggatttttta gcttctttac tgagatggat tttccttttt agttcgtgcc aatctcaagg
      241 cacgatttgg attgagacga ttaaatagtt cttccaattt cttttcattc caaacgtctg
      301 cggcggaaac aaaattacca tccgcatctc ggaaagatat cggtttatct cccataccag
      361 tctcctagtc tacaaattca aactcaaatt taccaatgcg gaccaaatcc ccatctttag
      421 ctccacgcgc acgaagggct tcatcaaccc ccataccacg aagctgacgg gcaaatttca
      481 tgacagattc atcacgatca aagttggtca tattaaagag tttcatgagt ttttcaccag
      541 aaagtaccca tgtcgcatcg tcatcacgac taatttcaaa ggctttttct tcttcgtcaa
      601 atccatagta agcttcttct tccatatcgg actcgtcgta gagcaaaaat tctggtgtct
      661 tgtctaacaa ttcagctgta gcatctaaaa gtgttaccag accttgcttg gtcaatccag
      721 aaattgggaa gatagctggt aactcttcaa attcatcata attttcagcc aattttttct
      781 taaattcttc aagattttcc tgactctcag gcatatccat cttattagct acaataatct
      841 gtggacgctc catgaggcga agattgtaag actccagctc tttattgata gctaggtaat
      901 cctcatatgg atcacggcct tcgctagctg acatatcaat gatgtgaagg ataacacgtg
      961 tacgctcgat atgacggagg aactgagttc ccaaaccaac accttgacta gccccttcaa
     1021 tcaaacctgg caagtcggct actgcaaagg attcacctga ttgggtgcga accataccta
     1081 aatttggtac aatagtggta aagtggtagg caccaatttt aggcttagct gaggtaataa
     1141 cacttaaaag tgttgacttc cctacagatg ggaatcctac taaaccgaca tctgccaaga
     1201 tttttagttc caattgtaac tcacgttcct gacctggttc tccattttca gagatttccg
     1261 gtgcaggatt ttttggtgtc gcgaaacgaa tatttccacg tccaccacga ccaccgtggg
     1321 caacgataaa ttcttgccca tgttcaatca aatctgttaa aaccttgcca gtctccgcat
     1381 cacgaacagt cgtaccttgt ggtactagaa ctctaaggtc ctcagcacca cgaccatgca
     1441 tccctttggt catccctttt tcaccagaat cagccttgaa atgacgattg tagcggaaat
     1501 ccatcaaggt acgtagtcct tcgtctacaa cgaagaccac attgcctcca cgaccaccat
     1561 caccacccca agggcctcca ttagggacat atttttcacg acgaaaggca accataccat
     1621 cgccaccatt accagccttg accttaatct tagctgtatc taaaaacata ctcattattt
     1681 cttctcactt taaaaaaggg ctgggaaatc ccagtcacta aattttcttg aatctatttt
     1741 atagattact gagggcacca attgcagttg caaaaattcc caataaactt gctactagca
     1801 tgataatcac gataaacaag gttattttct caaacatagt ttttttacga tttccattat
     1861 ctccaaatgc catttttctc tccttcgtta cattctattt tctattatct tagcatgaat
     1921 tgagctagtt ttcaatagtc agttgctact ggtgcgataa tccataaaat aatgtaagct
     1981 acaattccag ctccgtaaca gcaagtaaga acaccccaaa tgactcgaac gatagtcgga
     2041 tccatatcca gataataggc caccccagca caaacacccg caatcttttt atcacgaccg
     2101 cttctcatta attgtttttt tcatagctgt tcctctttct attcttcatg gtctttccaa
     2161 tagtctctca tactgatcaa ctgtgttttt gaagccttca acatgcgtct agaacctttt
     2221 acaggtacag caaccacctg ttgcggaaga tacaaaactg tcttaaagat acgctgactg
     2281 accgtatcat ctttagccaa atctttctgg aagttatttg aaataatagc atccagataa
     2341 aactcatgca ctcgtttccc aaagttctca gctgaaatct catacaattt ctctgataag
     2401 gtatgctcgt tcatgtctgg tgttgcaatc agggcttcca aaatagcacc agccaaatca
     2461 tgttctccat agtacaaggt tccaaacatt ttatcactga tgaggttgtt caaataagga
     2521 tttccgtgag caatgacagg tgttccactg gctaagcttt ccaagtaggt caaaccttgc
     2581 gtttcgcttg tcgatgccga aatgaagaaa tccgccgctt tatagtaaag agccgtctca
     2641 ctaggagcaa tcatccctgt aaagatgact gagtcttgaa tctctaggtt ctgagcttgc
     2701 tctttgaggt cattcagata agggccatcc ccagctacta ccagtttaac cttgtcttcc
     2761 tctttcagaa catcagcaaa ggctgctaaa actgcttgaa tatttttttc ataagagatt
     2821 ctcgaaagac taagcaacgt cttttcacca tcttgaatcc ctagtttact acgcagttct
     2881 ttcaaatttt cctgcttgat ttccggacgc tcaaacttgg ctaattcaat cccagtagga
     2941 atgacccgtt tttcaacctt gaccttataa tcagatagca agtcacggac aatctcacta
     3001 ggacaaataa ccccatccac atcatgcagg aaacctctaa ccagatactt gaccatactc
     3061 ggccggatca acatcccctt agcaatataa tggacatagt cttcatactg ggtgtgatag
     3121 gtatggatga ctggaatttt caattcacgc gcaatccaaa tccccaacag gccaagagaa
     3181 aattctgtct gagtatggat aatatctagc tgatactgtt tagcaatttc aagtgccttg
     3241 ctaaaacctc ggtaggcaaa gcgacgatcc ttaaaagcaa agaaaggaac acttggaatg
     3301 cggataattt gccaatcttc gtagcgattg acatccttat ctgtcgtcgt aaagataaaa
     3361 acagcatgtc cctgcttttc aagttctgtt ttcaaggttc gaatactggt cgcaacacca
     3421 gaaacctgag gaaaataggt atctgtaaat aaaccaattc gcatagatta cctcactttt
     3481 tactttctcc ctaaagcggc ttgtttctca taaaagtcca accagatttg taacagattc
     3541 tcttcagaat actctctgga aatattctta gccttttctt tgagatcttt taaggcagca
     3601 ggatttgctt gatattccaa aatagcctct ttcatctctt ctctacccgc tgtcgcccga
     3661 taatttccct ccaaaatcac cttatagaga tctaaatcac gcaacataat aggagcctca
     3721 caactcgcag cttctaaaat agtcatagga aagagctcat tgtaactagg caacaagaaa
     3781 agatccgcta gagcatacaa ttcgcgcatc cgctctggcg atacaatgcc tggaaaaatc
     3841 aaatttttag ggggattttc cataattttc ttatagtgtt cataaccatc tgtcatacca
     3901 ccaaaagaga agccaccagc ccagataaag gtaatctgag gcaattcctc agccagacgg
     3961 ataaagtcat caatcccttt acgtttctga acttgcccag cacctactac gataaactga
     4021 ttgtcactaa gaccaagatc tgtgcgcagt ctgactacct cttcttgtgg tagaggatgc
     4081 catttttcct tgttgacaaa gttaggaata taggtcactt tttcacgtgg aataccagct
     4141 gctaccaaat cctcaataaa cataggattg accacaacca agtgctccat ccggttgtaa
     4201 aaagaaaata catagcgttt cacaattccc tttaagaaaa atggaatttt caaacttccc
     4261 tcaagtgtag ctggcaagaa atgcacatag ccaatctttc tccctgagcg tttcttttgg
     4321 aaggttgata aataataggg aaaatcaatc gtatgaaagt gagtcacatc tgcctcgatt
     4381 ggaagatttt ctgtaacaat caattggtcc ttggcagcac ggtgaagaag acgaactaat
     4441 tcacggtaag cacctgaaac tccctgtcct gctactttct cacttgaact caacatattg
     4501 atgcgtaatt tctttttctc cataactact attatatcat tttctttgaa taaaatcagc
     4561 aaaagaaagg agagactaga ggaaaagagg ggaaatttcc tcgcttttcc aacagtctct
     4621 cacatgcttt tatatgttta cttaatgcct agggcaatgc gggcatagcg actcattttt
     4681 tcaactgtcc aggctggata ccagactaat ttaacctcag tatccgttac ttctggaacc
     4741 tctatcatag catcataaat ctggtctgtc aaaaggtctg ctaagggaca acccatagtt
     4801 gtcaaagtca tgtcaatctc tgtttgccct gtgtcaccgt caaaacgaat ctcatagatc
     4861 aaaccaagat tgacaatatc gattcccaac tcagggtcga tgacttcttc caaggctgtt
     4921 aaaatccgtg ttttgatgtt ttcaatttgc tcttctgtat aagccatatt ttcctcactc
     4981 ttagtcttca ataaaatcac gaagcggttt gctacgactt ggttggcgta gttttctcaa
     5041 agcctttgct tcaatctgac ggatacgctc acgagttacg ttaaagactt tccccacatc
     5101 ttcaagtgtg cgcatttttc catcatctag tccaaaacgt agacgcagaa cattttcttc
     5161 acggtctgta agagtatcta agatttcatc caattgctca cgcaagacga tacgagtcgt
     5221 ataatccact ggattttcaa tcacttcatc ttcgataaag tctccaaggt ggctatcgtc
     5281 ctcttcaccg ataggagttt caagagatac tggttcttgg gcaatcttca agatttcacg
     5341 aaccttatca ggtgtcatat ccattcgttc agcaatctgt tctggtgtcg gatcttgccc
     5401 caattcttga aggagattcc gctgttcacg aaccaattta ttgatagttt caaccatgtg
     5461 aactgggata cggatggtac gagcttggtc cgcaatagca cgagtgatag cctgacgaat
     5521 ccaccaagtt gcataagttg aaaacttgaa ccctttagaa tagtcaaact tgtcaaccgc
     5581 cttcatcaag cccatatttc cttcttgaat caagtcaagg aactgcatac cacgaccgac
     5641 atagcgtttg gcaatggaaa caaccaaacg aagattggct tccgcaagac gttgtttggc
     5701 ttcgatatca ccagcttcaa cagccagtgc caactctttc tcctcttcat tggtcaagag
     5761 aggaacgacc cctatttctt tcaagtacat acggacaggg tcattgacct tagcagaagt
     5821 tgacccaatc aagtcctcat cgctgagttc tggttcttct tcattgctga gaacacgcgc
     5881 acttggattt ccttcgttat ctgtgataga aatgcctgca tcctgaatcc gttgcaagag
     5941 atcttcaatc ccatcagcgt ccaaggtaaa aggaataacc agacttgcat tgatttcatc
     6001 atctgttgct gtcccttttt gcttatgatt acggataaat tctgctacct gtacgtcaaa
     6061 tgttgttact tctttttgtt ttgttgccat tattactcca ttcttctctt ttgggaaatt
     6121 aaacgttcca attcttctag ggctgtatct gtatctccta catggctagc ttcctgcacc
     6181 ttctttttga ttctcatatt gtcctgattc aagagagcct tgtttcgagt catctctact
     6241 tcactaagtt cctgcggcga tatctcagca ggcaaatcct gagctaaaac ttggtaccaa
     6301 gctctttcaa cttcctctgt ctgctctgct aaaacttctg gaggaagatt tccatactgg
     6361 ccaagcaagt catataagac ctgaaattca ggtgtagcaa atgcaaagtc ttctcgcaaa
     6421 cggtaatcgt tcaaaacaag aggggattcc atcatccgat agagtagatg ggcttctgcc
     6481 ctcataatag ccgataactg cttggtgaca ggcatggtga ttggcgtcgg tctggaaatt
     6541 ctttccatgc gattctgcct ttgcacctga cgactctcat taacaatctg ctcaatctgg
     6601 gtataatcaa aggacgccag actgtcagct aaaatatgaa tatagctgtt ttgagcagcg
     6661 atggactttt cttgaacaat caagggagct attttttcaa taaactcaat ctgagcctgc
     6721 agattttcac tgttttcagg tttgtactga tgaatgtaga actcaatcgg actaatacga
     6781 gtttttgtta atagataggc caagtcttct ggaccatttt tttgtagata ctcatcagga
     6841 tccaagttat caggcatgct aacgatttgc acaggcatat caccaatttc atccaatgct
     6901 ttcaatgtcg cggcttgccc agccttatct ccatcgtaaa caagaaccaa tttcttggtt
     6961 aaccttttca gatgctcaac atgctctcga ctcaaggccg ttcccatcga cgccacagca
     7021 ttttcgattc cagcccgata ggctgcaata acatccatga atccttccat caggtaaatc
     7081 tcactagctt ttccagaaga tctttttgcc ctatccatat gatataattc gtaacttttg
     7141 ttaaaaattg cagtcgatcg gctgttttta tacttagaag tttgtgaatc cgttttttgc
     7201 cagatacgac ctgagaaggc aatgaccttt ccttggtcat ttgtcagggg aaacataatg
     7261 cgattgtgaa aggtgtctac aaattgattg gcatccgaga gataaaacag gcctgaatcc
     7321 agtaaatcct cttcacgata ctgatcagac aaacgttgat agagatagtt tcgttctgga
     7381 ggtgctaaac caatccaaaa atgtttaagc acttcatctg tcaaaccccg ctgataaagg
     7441 taatttctgg cctcttcgcc catagtcgtt gtcatgagaa tagcatggta aaatttggct
     7501 gcatcttcgt gcatatcata aagatcttgg tgaggtgagg ctgacttttg ctcactataa
     7561 agcggttttt caacctcaat tccaacacgc tgacctaaga tttggactgc ctctataaag
     7621 ggaacccctt ggtactcctc tatgaactta aagacatcac ctgagcgacc acaaccgaaa
     7681 cagtgataaa actgcttatc ctctacaacg ttgaaagaag gtgttttttc accatgaaaa
     7741 ggacagagcc ctagatagtt ccgtcctgcc ttttgtaaag aaatcacatc tcctatgact
     7801 tccacaatgt tggcattgtt tttgatttct tcaatgactt gtttgtcaac catacacaat
     7861 acctccatgt tatcatagtt tactttatat agtatacttt atttcagaaa aaaagtaaac
     7921 catttcactc attttcctta ctttattcaa agagttgata ataatcagag attttcattt
     7981 ttgctttttc ttcttggttt aaatcttgga taatccgtcc ttctttcatg acaatcaagc
     8041 gattgccata tttgagagca tcttccatat gatgggtaat cataagggct gtcaattgat
     8101 ctttcttaac aaattcatct gtcaattcca tcaaagcaac actagtcttt ggatccaggg
     8161 cagcagtatg ctcgtctaac aagagtaatt caggtcgctt caaggttgcc atcaagagac
     8221 tcaaagcctg tctttgtcca ccagataaga actcaatcgg tgtattcaag tgtttctcaa
     8281 gaccgtttcc cactttttca atggtcgcct gaaacttatc cttatagcta gccagacgtc
     8341 tcggaaatag tccacgcttt tcaccacgaa acttggcaat caggagattc tctgccactg
     8401 tcatacgagg agctgtcccc atctttggat cttggaagac tcgagacaga tacttagcgc
     8461 gcttctctgg tgaaaacttg gtaacatcct cacctaaaat acggatagtt ccactggtta
     8521 gtgatatggt tcctgcaata gtgttaaaga gggttgattt accagcacca tttccaccca
     8581 aaatcgtgat aaagtcctgt tcaaaaattt ctaaggaaac atcatttaaa ataatctttt
     8641 cttcatcaaa gccattttta acgattttgg ttgcattttt taattctaca attgctgtca
     8701 tttgcttaac ttggctcctt tcaagattgt ttgcttaaat gttggaatca tgaggcagac
     8761 tgctaaaatc aaggcactgt ataaacgaag gtaacttgta ttaaagccaa gtgcgataac
     8821 tgcccacact aaaaattgat aagcgataga acctacaacg atagtaacca aacgctctgc
     8881 caagctcaaa ctcttgaaaa taacttctcc aataatcaaa cttgcaagcc ccacaacgat
     8941 aaccccgatc cctcgagaca catcggcata accttcttgc tgagcaatga gggcacctgc
     9001 aagggcaatc acaccatttg ataagaccaa gcccatgagc tccatgcgtc cagtatgaat
     9061 cccgaaactt ctagccatat caggattatc ccctgtagca atataggctt gtccgagttt
     9121 agtgtccaag aaaaagagca tgagagcaat aacaatactc acaaagatga gacctgtcaa
     9181 gagttgattc aaatccgaat caaaaggcaa aacatcctga atttgcttgg ttccaagcag
     9241 gcctaaattc gcacgtccca taatcaagag catgattgag tgacaagaag tcatcaccaa
     9301 aatccctgag agcaaggttg ggatcttccc ttttgtataa agaaggcctg ctgccattcc
     9361 agccaaacaa cctgctccta cagcaacaag tgtcgctaaa aatgggttca cgcctttggt
     9421 tatcaaagtg acagcaacag ctcccccaag agggaaggaa ccttctgtcg tcatatctgg
     9481 aaagtttaaa atcctaaatg tcataaagat tcccagacct agaatagccc agacaaatcc
     9541 ttgagaaata atggaaacaa tcatatttta tttaatcctt tctatattca tctttttaaa
     9601 aaatgggaag agtctcctcc tccctacctt atttattcga tgacttgtcc tgcttctttg
     9661 agaacagact caggaatagt aatacctagt tcttgtgcta tttttttatt gatgactgac
     9721 ttaccagttg aaaagacatt gactggggta tcggctggtt ttgcaccttt caagacttgc
     9781 acaatcattt tacctgttgc cacaccaagg tcatgttggt caattacaac tgatgccaaa
     9841 ccacctactt ctaccatagc tgtcgcactg ggataaattg gtttcttaga actttgattg
     9901 ctagagacaa ccgttggaaa tcctgatgca atggtgttat caattggaac ccaaatagca
     9961 tctaccttgc tagtcataac agtgacagtt gaggcaattt catttgttga aggaactgca
    10021 aatgtttcca ctgtcagacc tgctttttca gcataagcct taaattcttc gacctgtgtt
    10081 tttgaattgt cttcgctact tgagtaaaga gctccgattg ttttcacatt cggtgtcaga
    10141 gccttgatga gttcaacttg ttgttgagct ggattgtggt cagatacccc tgtaacgttg
    10201 ccacctggtt ttttcaaatc tttaaccaag ttagcaccaa ttgggtctgt aatagcggcc
    10261 atgataaccg gtaggtcttt tgttgcacta gccaaccctt gggctgctgg tgttgcgata
    10321 ccaaccacaa ggtcattccc atttgcaacc aattgtttac tcattgtcgc aaccttactt
    10381 tggtcacctt ctgagttcat aaaatcaatt ttaacttgat catctttata tccttcttct
    10441 gcaagtccat cttggatccc tttataaatc aaatcaaggg atggatggct cacaaattga
    10501 agcacaccaa tcttggttat tttcttctca tccttattat tctgaacttc tgatttattc
    10561 attgaagaat aaatcaagct tcctgctact aagactgcta atgcagcaat aattccaatt
    10621 aaacgtttat ttttcattct gtttctcctt tttatttcct ttaacatatt tcacttatct
    10681 aaacaagcga cgccatcgtt ttctttccat actaacctcc atcaaaaaaa tcctcacaca
    10741 aaaaacttgt gtgaggacgt cgatgcgcgg taccacctca attataggga atttccctat
    10801 cgctcagtct cgcaataacg agatgcactg taaggtgtgc ttaccgaatt tttataattt
    10861 caaattctaa atagcattca gcccaatttt catcattgcc acttatctgt tttcagctac
    10921 cacagactct ctaaaaagtt tgtatgatta cttttctgaa tggttaaatt atatcatttt
    10981 taaactactt gtcaatagtc tattagaatt tttttgatat tcacttttag cattcactct
    11041 tgaaaattat caacctgaat tacgcaatcc tgtcgcaatt ccgttgatgg tgatatgaat
    11101 caatcgttct tgatcactgg acaattctcc acgacgttgg cgtttaatca actctaactg
    11161 aatatagttg agaatattaa agtaaggcat acggtaatcc agactagctt ttagatatgg
    11221 attgtcagct aagagttcgt catgtccttc aatagccaag ataacgttct tagtaacttg
    11281 ccattcattt aaaatagtct catagatggc cttaacttgc tcgtcttcac aaagtttagc
    11341 atattcaaaa gcaatattca tatttgattt tgacaaaacc atatcaacat ttgaaagaag
    11401 cgattggaag aaaggccaat tttggtacat atctcgtaag atagcaatat tctctggatt
    11461 tttattgata aattccttga agcttgaacc aaccccgtac catccaggga acataacacg
    11521 actctgtgac catgagaata cccaagggat ggcacgcaaa ccaccgattt cagtaatagt
    11581 cttacgagcg gctggacgag aaccaatatt aaaacttgaa atagccttga ttggacttga
    11641 ctcgaagaaa taatcataga aatgctcatt accaaagacc aaatcacggt agatatcgta
    11701 actacggtcc actacttgat ccataatggc ttcataacga tttggggtat tggtatcgct
    11761 cttcttctga gtaatcatac ggttaatagc tgccgatact agcatttcaa ggttatagta
    11821 agcggcgtct ttgttaccgt atttattccc aattacttca ccctgctccg tcaagcggat
    11881 acgatccttg atagacttga gcggttgaga tgtaatggct tcataggttg gcccaccacc
    11941 acgaccgaca gtaccaccac gaccatggaa gaaggtaacc ttaacgccaa attcatctcc
    12001 aatagcagtc aattgttgtt gagccttgta gagggtccaa catgatgaca agtaaccgcc
    12061 atctttatta ctgtcagagt agccaagcat gatttcttgg tagttatttc gtgagtcaat
    12121 ccattttttg gcaaggctaa gagaaagata ttttctcatt gtttcctctg aatgatccaa
    12181 gtcttcaatt gtttcaaaaa ggggaacaat ctgaacacgc gccctttccg tatccaccag
    12241 tcctacttct tttaacagaa tagctaattc tagcatatca gaaaggctgg ttgcatgtga
    12301 aatgatggtc tgacggatga catcatctcc caacttatct ttcaaaacac gagccgtctt
    12361 aaaaatagct aattcttttg ctaataattc tgatttttct gcgtgagtcg cagaaagaat
    12421 tcggggatct tcttctaatt ctttcaagag aaggtcacac ttttcttctt cgctcaactc
    12481 gctataacga gaatgaattc ctgctgattt caagagttct gccacacagg cttcatagac
    12541 gctagagtct tgtcgcatat caattgatgc taagtaaaaa ccaaatatct ctactgcctg
    12601 caataattcc acaaaatcac ctgaaatcaa ggactcgccc ttattttcta gtagagaatc
    12661 tcgaatggca atcaaatcct tgtagaaatc attggcggtt tcataacgag tcccaacttc
    12721 ttcatcctca atcagatagg ttttagttgc ctgaattttt gattgaatat caaacaaggc
    12781 acgacggtaa agctcttttt cgcggtaaat cgagttatcc ttggattgac gagccatttc
    12841 tctgacttgc ttgctgacgt tgacaatgct agttgaaaga gaaaattcac gataaagttg
    12901 gtaaatcttt ttatcatagt agttcatgat gacttcacac tgagtgagtg cagactgctt
    12961 caaggtcttt gctgtaacaa atggatttcc atcacggtca ccacctatcc acatacccat
    13021 ggtgattggt ttagcctgtt ttaaattcag accatgcgct tgcgctaagc gcttatactc
    13081 cgtcgtcaaa tgaggtacag ctttcaaaaa ggagctgtta taatattcca tagcattcgt
    13141 gatttcgtta gtcactttta attttttctc acgaatcatg tctgtctgca tgataatttc
    13201 gatgtaacga cgcaaatcat tgtaccattt atctttattg atcaacccca acttaacatc
    13261 acggtattta cgcaaaagac tatgaatatg atttgttaaa tccaacatac ttttgcgttg
    13321 cacttgtgtt ggatgggctg tcaaaacagg gacaacattc aagtgttcta ggatctcaac
    13381 ggcattttcc ttttctgcta ccaatttaat cgttgtagat aatttaccta aatagtcctg
    13441 atcaatatta ttttgatgat tgatttcata agctaaatcc acatcctctg aaatattaat
    13501 caaaagaggc aagatagaga aatagcgtga aatatagacc atttcatcat ttgataagct
    13561 agtcactaga cggtttagac cttgataatc ttcctgcgtt gataattctt tcaactgtat
    13621 tattttttca aaggtctctg gggcaagcat atttttagta atatcttcca gtaattctgt
    13681 tagaatcaag acttcttctt gcacaacact tttattacta taattttcta atttttgaag
    13741 agacataaac tatcctttct ttatcctact tcaacagaag gttcattggt tgattttcca
    13801 attctcggta caatttagcg cgtttttcac tggcatcaat atttaagaca aaggctactg
    13861 ccactgataa gactagaaga ctatttccac cctgggataa gaaggggaaa gtcactcctg
    13921 tagatggaat caagcccgaa atccctccga tattgacaaa tacctgaacc aacatcatcc
    13981 ctccgacacc gagtgcaacc atggcattga aaggattctc cgctcggata ccgaccaaga
    14041 taatccgcaa aatcatgaaa aacaagagag ctaaaataag actggcacca acaaagccaa
    14101 attcttcaat cacgatagaa aagacaaagt ctgtatgagc ttctggcaaa taacctcgtt
    14161 tttcaatcga gtttccaaga cctagaccaa accaaccgcc attgaccatg gcaaaataag
    14221 aattagctaa ctggtgacct gcatcagcac gatcggcaaa aggattaaaa aaggcactaa
    14281 agcgcttggc tacatagccg aatactggaa tttttgaaaa ggtctcaaca ccgattaggc
    14341 tgatagtggt caagacaaag acagaagcgg cagatacgag cgccagaatg gttgaaaacc
    14401 agcgataagc gattccacta actgtataca taatcaagga aaccaagact aaaatagtcg
    14461 catttcctaa atcagggaaa attcccaaac ttccaatcag aactaggaga acgaatcgcc
    14521 aatcattaaa agcacgggga agccattgat tttgagtcaa aacttgaaaa tcataagtag
    14581 ctatttcttc ttgctgtttg gagaatcggt gagctaaata ccaaataata atgattttta
    14641 agtactcagc tggctgaata gttactcctg caaccgaaat ccaaccgtat gccccgttta
    14701 cggaaagacc aataaaacga gccaagaaca atagaagcat ttctattaat ataactaaaa
    14761 tgattagtcg ctcatttctc aaaaaatcta gtctcaattt ataaattaag gcaatcagta
    14821 tcaaactaac aatccaaaag attccttggt ttcgaaccaa ctgcaaggca ctcttgcctt
    14881 cttcaattaa aatagcactg gtggtcgaat agaccacaat caagcccaaa atagataaaa
    14941 gcaagtaggg aatcaagatg gaataattta ataagtgcct cttactaatc ttcatagcat
    15001 caccactcta ctaggataca gacaaatctg ttccaaattc cgttttattt tctagttttg
    15061 attgctatta taccattttt tcagaaagaa aaaaactctt accttataat ccttcgaatt
    15121 ttactgtttt tcataattag agcagaattt gaaatcgtaa aaccattttg aacaagagtt
    15181 acttcaaccc attgactccg acggattaag ttgctttcgt gaatgccaaa atcagccgaa
    15241 atttgttcat aagtgcgcta ttctcgcatg tattgaatag ttaccataag gagatcgtct
    15301 aggcttaatt tggtttttcg tccacctttt gcgcgtttac gttgataagc tgtttttaac
    15361 acagctaaca tctcttcaaa agtcgtgcgc tgaacaccta caaggatctt gaaacggcta
    15421 tcgctcaatt gtttacttgc ttcatcatcc atagttttat tgtattgtat ttttgtttcg
    15481 caggaagtct attattcctt tcgttccaag gtttttacgc ttttagcaac aagcttacac
    15541 actgagcgtt tcggacttac catatcattt cgttggagat tttatcatca atttttagat
    15601 taaaaaagat ttcccaatct tcacaaactt ctgtagcagg aattgtatga atctttctaa
    15661 gtatctttcc tgcttctaca ccatatgtgt attgttggtt ttatgaataa gttaaaatgc
    15721 tatctattgc atctcttccg tctatccatt cgtgtaaaga atgtacttcg tcatcgcaga
    15781 gttcaatgct aattggttta cacatcggaa ctccaagaga agctactttc tccatcgtat
    15841 caaattcaat ttttttataa tctaacttcg acttatcaga aacacgcaag aaatatgttt
    15901 actgattttg aactgtcaca caatattttt tatcatctga ccagccttta tttatggcta
    15961 tcttactact aaaatccatc aagcacactc cgattcactt tacaacactt tttgcagttt
    16021 tcactagtaa cctatccagt tcgtctacac ttttatttcc tagatccatt tttacattat
    16081 gaaagttagt caattttctc tatgatgttt ccaagtttat ccaatacaag acaagaatga
    16141 tcatttagtg gaattatggg aacattaacg agttcttcag ctctatcctt atttgcttta
    16201 tcattccagg aatcataatg gacactaatt aaaaactgac taaaaagtcc taatccattt
    16261 tttatcttta tctgataatc tgaattaaca ttaggtgaga catagacttt ttctcctaat
    16321 agtagggctc ctgcagaaaa tcctataatt tttgcctctt tattaagcat atgatcgata
    16381 taacttttga actcctgatt aacataagta gcaatatatt tttccgtatt tccaccaccg
    16441 atgataatta tgtcagcatc tagatagcta tcaagatcaa tctgctcagt gtccaggagc
    16501 aagtaatcaa tgtttagatt aggaaattga ctttgaaaaa cttccgtgta ctttttcatg
    16561 tacggttccc aattttctcg aaaaactgta aaaatgacaa ttctttcgat cttccttgat
    16621 gacacaattt tattaacaat cgtatagttc tttatcggcg gattcccacc catcaaaaaa
    16681 atttgttctt tcaagttatc acactttcta aagatacata aagtttaaac aagtttattc
    16741 acgtttcgaa attataattt ttttaaaaca tatcatttta ttgactggct aagatcgaac
    16801 cagcaacact acacactcca cgtgtgttgt ctgttccttg agaatacaat tttttatcct
    16861 cgtcactgtt ttgaggacac aatttatctg tttctagttt agcattttta cgtttacttt
    16921 taaattgttt accatccttg ttttgaattt cacctgtttt gaatattatg gttaacattg
    16981 caggatcaat ttctccgttg ctattaactc cacctacaat aattttctca acaatacttt
    17041 caaaaactgc acgatcaaac tccgtaagca ttttttgcga agatagcaag tgcttaaact
    17101 cagttagtct tttctttaca tcgatttctg attttaatgt tgtttcaata tttaccttct
    17161 cttctgctag aaactctttt tctgaggaaa tttcattgta cttttcattg tatatggaat
    17221 cgctaacttt tccttcgagc cttagattta caagattctc ctcttttttt agtaatattc
    17281 gaagtttgtt tgtaatcctt tttagttcct tgttaaggct attgtcattc aattcagatt
    17341 ctatcgtttc aagtaagtct gtcattaaat tttcatttga atgatagact tgtctgtaag
    17401 cttccaggaa agccccctca atggcgatct cttctaatcc tttactatgc ttacaaaatt
    17461 tctttccttt ctttatcgat gtaacacagt gccacacaac tttgcgataa tctgaacggc
    17521 agtgccaact tcttctagat aatattgaac cacaaaaacc acattccaac atgctactaa
    17581 aagcatacat ttttgaatag cgttcacgct tcccatttac attcgcagct gtcttcttat
    17641 ttcctgctcg acgcaatcta atctcctgag ctttttcaaa atcttcttta gatattattg
    17701 gttcatgatt atccttgata tagtacttat cttcttctcc aaaattactc aaccgtctct
    17761 tgcttattgg gtcaactgta aaagtttttc ccattagaat atcaccttta tatttttcat
    17821 tctttattat tcctagaact gttgtatcat tccagtgttc taaacctctt ggcgatttgt
    17881 aaccaagttc atcaagttcc cttgcaatta cttttcctcc aataccttct agataccttt
    17941 caaaaatata acgaacaatt ttggcttctt ttttattaat tgaaatctgc ttggtttcta
    18001 catcataatc ataccctaag cacccttgaa aaccaaccaa ttcaccacgt tgcatcttca
    18061 tttttaatcc ttttttcaca tgggcagaag tattctctac ttcttgctgt gcaacggagc
    18121 ttagaatagt tagaagcaat tctccatcca ttgttagggt atctatgttt tcttcctcaa
    18181 aatatactcc aatttgcata tcctttaaca ttctgacata cttcaaagta tctagggtat
    18241 tccttgcaaa tctagcaatt gcttttgtga caatataatc tatctctcca tttgcacaat
    18301 cattgattaa tcgttgaaaa tcttgtctct ttcctacttg tgttcctgaa ataccttcat
    18361 ctgcataaat atctgccagc tcccaatctg aacgctgaga aatatattct ttgtagtgtc
    18421 ttacttgaga atcataactg tttttctgat cctcactatc ggaactaact ctgcaataag
    18481 cagcaaccct ctttaagtgt ctttctacat tgtcgcctct tcgacgaact aaagaatcag
    18541 cttttattat ttcaacatta ttttttttca tactgaaatt cctccttttg attctatatt
    18601 accgcattca aatcaaaaag tcagctattt tgtgtactta atacgcagtt tgtttctaat
    18661 cttgtcatac tcatttttag ataacatatt tcttgagtat aaaaagttaa gcattgatag
    18721 ttgcatagta tactttatta ctgattgttc caaacttttt cctccttgta acatgtataa
    18781 tttcctccgt gggtatgact ggttaaatca ttcatgggca tttcacccgc tgtccttttt
    18841 cgctggcagc ctgaacgttc agaagtatca ttatccttat ttgtagtcat ggcagataga
    18901 gaaccactct attttatagc taataattat cgctcctatc acggcgtatc aagctaaagt
    18961 gctcgacaaa taagaaagtc ccacctcggt ttattcagtt ttcaaagggc ttaggattta
    19021 ttaacaacta aacttatcca tctctaaaat ataatctgta gttacctgat ataatttagc
    19081 taattgtttc aaaaatgatg taggaatatc ttgcttgcca ctttctattc tagaataagc
    19141 agactgtgaa caatgaagat actgtgcaat ttgacactgg gacaaatctt tgtcttctct
    19201 taaattcttt aatctgatat acattaataa tcctcttact aatattgtaa tttgaagatt
    19261 atcaaatttg gaattttatg caaaatatgc ataaaataat ttataagatt ttagaaatag
    19321 ttggtataac agtaagtaag ctataagtta ctaaaaaaag attttggaat aaactttttc
    19381 atagcgataa aagcaccgta caagctagga aaataataag taatcgccca atctgacctt
    19441 ctccagaact tgattattcg tcaatcacta cagttgacaa acatactaag attagtagtg
    19501 aggaaatctc caacgggaga gagtactcat tactttttct ttgtgttaaa atagtggtgt
    19561 cttgttaagt catagagctc tctatggtgc tagacgtcgc ttgacaaact ggcaagaaac
    19621 ctgttttagg aagaatttta tcaaagtatg tgggatacta gacgtcgagt attggaaata
    19681 aaatcactaa aacaaggagt cattcaagac aaaggggtaa acatgatgcg gacagttttc
    19741 agaatggatg tgagtaaggc aagctcagaa gtcgctattc ttgtgaatgg tgaaaaggtt
    19801 catggctata ccatgcccaa tgatgccatc ggatttagtc gtcttctaga ggatttgaaa
    19861 taggtaaata cacctgagat tatcttcgag gcacaggggt ctattctcgt cttcaggcct
    19921 ttctggagga caatagctat gcttatatac ggctaaatcc tttaaaacta agaagcaact
    19981 agacagtttg cgagtccgta aaaccaatca gattgacgct gaaaagctag cttcgtcaca
    20041 attcatcctt aaccgcaaac caacttatgt tcaagaagaa gtctatcaaa atttgcgtga
    20101 cctcagtcga ttctatcaga atctgacaga agacatcgtt aggactaaga atagcctgca
    20161 caaggtgctt caaattgcct ttccagaaat aaagaacctt ctctctactc ctacagggga
    20221 acaatattgg aacctcgtga ccatctttcc aaccaagcat tggaggctga aattatcagt
    20281 agttgagcta aaagaagcca tcgtaattca acttctaaac ggatttctga gaacagagtt
    20341 ggacgattgg ctgaaaagtt gagcgagctg gccgaacagt cctatcttgc catttcaaaa
    20401 acctctccca tgacagaaga ggttaagtat catgcacagg aactgttgag actgacaaac
    20461 cgcaaggatc aggacttagc acaaatgatt gaattggctg aaccattacc tgaatacgag
    20521 attctgctct ctattcctgg tatcgcagaa acaacagcta caagcattat tggcgaattg
    20581 gagacattcg tcgctttcag tctgccaacc aaatcaatgc ctttatcagt attgacctca
    20641 gacactatga atctggcaat ttcctagctc aagaacatat caccaagcgt ggaaatcctt
    20701 acgcgagaaa aattctctat aagtgcgtcc acaatattgc ttcagctagt cataccaatc
    20761 cttgtcatat cgcggacttt tatgagaaac gaaaaagaca atcgcagacg acttcaacca
    20821 agccatatac gattgtctct atacatcgcc tcatccggac aatgtattac ctcataacgc
    20881 ataacaaact ttacgactac actttaaccc aaagtcatta aagtcatcta tgtcctatca
    20941 ttgtaacacc ttatttttta ataccagata agtgtctttt aagtgtgccc aaatttcaaa
    21001 aaacagctcg caattatctt ctaaacattc ctttgatact cattcagaaa accttttgag
    21061 ctagaatatc gtatgtccct taacaaatgg tatgtataaa agagctaaaa aaccactcat
    21121 gatatgaacg agtggttatg actgaaatat tacatacctt tgtatcattt ttgcacaatg
    21181 gtcaaatttc ttgtttgaaa aactacagta agcctaatca aagctctcaa tgtatcatta
    21241 aaacttcctt ccaaagaata ggaagttcta tcaaaattct taattagatt ttacttcttc
    21301 taagaccctg cgctctcata tgcatccaaa cctttgttcc ataattttaa tgataaggta
    21361 aaaaatatta atgagataag gatcaatcca ccaatattga acaatccatc cttgtctttc
    21421 aagaaatagc tagctgggta ataggctgta aaagcaaaag gaatgataaa actaatcaac
    21481 caacgtaaaa acgaatggta aatcgcaatc gggtattttg caaaatcatt aaacatgtaa
    21541 aaaatataaa ttattgcccc agattgttta gtccaaaaag caatactagc tgttacaatt
    21601 tttaaagagg tataaattaa ggttgcaaag ggaatactga ttaaaaagag aaaaaccttt
    21661 gcccaagtcc aagtaagact agttattgtc attaacagca ataagacgcc tactaacaat
    21721 tctccaagag catcaatttg aaacgtttct actaaaatat gaaacaaagg gctaatgggt
    21781 ctagtcaaat acttatcaaa ttctccttta cgaattaaat gttgtcctaa agctcacaga
    21841 ttatcaaaaa agagatggtc aattccctta ggaatgagag aaaaaccata aataaaagca
    21901 acctgatgaa aactccagcc atcaagtaag ggaatatgtt gaaacaaaat atttagaaac
    21961 agcatgttta acccttgtgt caaaaagaca cccacaacac caactaaaaa atcagcctta
    22021 tactccataa tctgtttaag atattgtcgg ataaaaatca ggtgcatgcg ttgatatttc
    22081 ttcattctaa cctccttgaa tagttatata attctgtacg cacttccaaa tcagttggga
    22141 taaggcaatc ataataaaca gccagaataa ttgtagcgag agcgcctgaa tcatttgact
    22201 catgctatac ttttctataa taaccatcac cggggtatag attaaagaag aaaatggcaa
    22261 aaaagataat agttcggcaa taatttttgg aaaaaatgtc agaggaacca aacttccaga
    22321 taaaaaggca actaaagaat ttttcaaaag atttgatccc cataagtttt taaacacaaa
    22381 agctgaaaaa ccaaagcaaa tattgaaaaa aaaaattaat caaaaaagct aagattaagc
    22441 taagaaggta aactgtggtt attaaaacaa tttgtaagaa ggactcacct gataagagct
    22501 ttagacctgc aatcagaatt acaaatggta gaccaacact aacaaaaact agccatctag
    22561 acccaatttc tgtgaaaaga taagacattg caaaatgaac tggacgaagc agtcgcataa
    22621 taatagagcc atcctttact tcccatccaa tcataaaaga actatctgat tttgtcagta
    22681 gattcgtcac aaaactcatg atgatatata aggtcatatc agataaagta aaaccttgaa
    22741 tgagagactg gtgagatgaa tcaaacacag ctttccacaa gtaaaatgct acaaaggccc
    22801 ccatcacatc cccaatgcga taaagaaaaa aattgacacg atacgtgatt aattcttgga
    22861 ttccagcgct aacaaaaggc ttatagcgtt tccataattt agtcatccta aagctccttt
    22921 cgatagaagc gacgaataat atcttcaata ttcgcatccg tcatctttaa atcacggatc
    22981 gtaaaatcag acaaagtttg ttgaataatg tcagccgttt gatactgcga gctgtcgtac
    23041 tgaatatcaa gtgacaattc ctgtctcgtc acatagatat cagaaagtcc ttcaaaatga
    23101 gagacaatat aatcctgccc aggatgtagt tcaaaagaga gagtcttcat cttcccgaaa
    23161 gtctttttca gttgattcac agttccatca aagatttctc ggcccttatc aatcataaaa
    23221 atacgatcac ataactgttc aatatcacct aaatcgtggg tcgttaaaag tatggtcgtc
    23281 tcttcttctt gattaatctg tgtaatggca cgtcgaatat tatccttgac cgaaacatct
    23341 aaaccaatag taggctcatc taggaaaaga actttcggat tatgcaataa ggaggccgca
    23401 atatccgccc tcatacgttg acctagagat agagttctga caggatcttg aataaattcc
    23461 ttcaaatcta aaacttcatt tagaaaatcc atgcgttttt gaaagaccgc atcaggaaca
    23521 tcataaattt cctttaaaac cgagtaagtc tcctgcaaag ccaaatccca ccaaagttga
    23581 gttcgctgcc caaaaactac accaatatct ttaacatagc cttgacggtt ctcttgagga
    23641 atctttccat tgatacgaca aaaacctgat gttggtttca aaatccccgt caacatcttg
    23701 atagttgttg atttccccgc tccatttgcg ccaataaagc ctaaaatttg tcctttgggc
    23761 acctcaaatg ataaatcttt aacagcttca aagacttgtc tcttgggatg aaaaaaagag
    23821 cgaaaagctc cctttaagcc aggttccttt attgttttca caaaattttt ctgaagatgc
    23881 tctactgtta tcatttttat aaatcactcc tttcgtttca ctcaagttat aatatcaaca
    23941 aaaattgtag catactacag taaagttaag aaaatttacc atcattacaa accatatgaa
    24001 aaaagctact aaaactatgc catcttgtat tttatctgta agtaagctaa attactcaag
//
LOCUS       DAVLYV010000021        24009 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLYV010000021 DAVLYV010000000
VERSION     DAVLYV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24009
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.021"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            416..2569
                     /locus_tag="VYO70_000884"
     CDS             416..2569
                     /locus_tag="VYO70_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1713140.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2582..3931
                     /locus_tag="VYO70_000885"
     CDS             2582..3931
                     /locus_tag="VYO70_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1713141.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATIEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4101..4808
                     /locus_tag="VYO70_000886"
     CDS             4101..4808
                     /locus_tag="VYO70_000886"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1713142.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            5010..8735
                     /locus_tag="VYO70_000887"
     CDS             5010..8735
                     /locus_tag="VYO70_000887"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1713143.1"
                     /translation="MDKRIFVEKKADFQVKSESLVREFQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8828..10270
                     /locus_tag="VYO70_000888"
     CDS             8828..10270
                     /locus_tag="VYO70_000888"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1713144.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10307..11329
                     /locus_tag="VYO70_000889"
     CDS             10307..11329
                     /locus_tag="VYO70_000889"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1713145.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11326..11871
                     /gene="purN"
                     /locus_tag="VYO70_000890"
     CDS             11326..11871
                     /gene="purN"
                     /locus_tag="VYO70_000890"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1713146.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11955..12464
                     /locus_tag="VYO70_000891"
     CDS             11955..12464
                     /locus_tag="VYO70_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1713147.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12489..14036
                     /gene="purH"
                     /locus_tag="VYO70_000892"
     CDS             12489..14036
                     /gene="purH"
                     /locus_tag="VYO70_000892"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1713148.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFSDNVEEIAKAGIKAIIQPGGSVRDQESIEAANKYGLTMVFT
                     GVRHFRH"
     gene            14158..15420
                     /gene="purD"
                     /locus_tag="VYO70_000893"
     CDS             14158..15420
                     /gene="purD"
                     /locus_tag="VYO70_000893"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1713149.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVIYAGLILT
                     ADGPKVIEFNSRFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTVATVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15823..16311
                     /gene="purE"
                     /locus_tag="VYO70_000894"
     CDS             15823..16311
                     /gene="purE"
                     /locus_tag="VYO70_000894"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1713150.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPIIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAAIFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16298..17389
                     /gene="purK"
                     /locus_tag="VYO70_000895"
     CDS             16298..17389
                     /gene="purK"
                     /locus_tag="VYO70_000895"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1713151.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLVDKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEKYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLISNEPDSVDELGEGIDF"
     gene            17399..17626
                     /locus_tag="VYO70_000896"
     CDS             17399..17626
                     /locus_tag="VYO70_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1713152.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIGKEEFE"
     gene            17689..18987
                     /gene="purB"
                     /locus_tag="VYO70_000897"
     CDS             17689..18987
                     /gene="purB"
                     /locus_tag="VYO70_000897"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1713153.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19042..22980)
                     /gene="strH"
                     /locus_tag="VYO70_000898"
     CDS             complement(19042..22980)
                     /gene="strH"
                     /locus_tag="VYO70_000898"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1713154.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGGMVAAWTDTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRY
                     TAESVTAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASDLGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVLGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVTEKKLPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(23295..>24009)
                     /locus_tag="VYO70_000899"
     CDS             complement(23295..>24009)
                     /locus_tag="VYO70_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1713155.1"
                     /translation="AIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSITV
                     VRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNKL
                     VYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRFK
                     LDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYSE
                     TYKRSDYYKIKFISCDRDH"
BASE COUNT         6524 a         4774 c         5716 g         6995 t
ORIGIN      
        1 tttaatgcta taatgtctat tataaatagt ttttagaaat actaaaattt ttttctttac
       61 ttaataatga tatgaaatca tttcggtttt gttaacttgt aattcttata ttttctaggt
      121 agaacgtttg ttctgcctat ttttttatcc aaaaagtgca gttgggaggg agataggctc
      181 atttgggaag gaagtccagt ttttgtttag tgattggggt aagatagttg ttatcagatg
      241 agtttatact cttcgaaaat caaattcaaa ccacgtcaac gtcgccttgc cgtatatatg
      301 tgactgactt cgtcagtcct atctacaacc tcaaaacagt gttttgagca gcctgcggct
      361 agtttcctag tttgctcttt gattttcatt gagtattagg gaaaaggaga tgaatatgaa
      421 atttgggaaa cgtcactatc gtccgcaagt ggatcagatg gactgcggtg tagcttcatt
      481 agccatggtt tttggctact atggtagtta ttattttttg gctcacttgc gagaattggc
      541 taagacgacc atggatggga cgacggcttt gggcttggtc aaggtggcag aggagattgg
      601 ttttgagacg cgagccatta aggcggatat gacgcttttt gacttgccgg atttgacttt
      661 tccttttgtt gcccatgtgc ttaaggaagg gaaattgctc cactactatg tggtgactgg
      721 gcaggataag gatagcattc atattgccga tccagatccc ggggtgaagt tgactaaact
      781 gccacgtgag cgttttgagg aagaatggac aggagtgact ctttttatgg cacctagtcc
      841 agactataag ccttataagg aacaaaaaaa tggtctgctc tcttttatcc ctatattagt
      901 gaagcagcgt ggcttgattg ccaatatcgt tttggcaaca ctcttggtaa ccgggattaa
      961 cattgtgggt tcttattatc tgcagtctat cattgatacc tatgtgccag atcagatgcg
     1021 ttcgacacta gggattattt ctattgggct agtcatcgtc tacatcctcc agcaaatctt
     1081 gtcttacgct caggagtatc tcttgcttgt tttggggcaa cgcttgtcga ttgacgtgat
     1141 tttgtcctat atcaagcatg tttttcacct ccctatgtcc ttctttgcga cacgcaggac
     1201 aggggagatc gtgtctcgtt ttacagatgc taacagtatc atcgatgcgc tggcttcgac
     1261 catcctttcg attttcctag atgtgtcaac ggttgtcatt atttcccttg ttctattttc
     1321 acaaaatacc aatctctttt tcatgacttt attggcgctt cctatctaca cagtgattat
     1381 ctttgccttt atgaagccgt ttgaaaagat gaatcgggat accatggaag ccaatgcggt
     1441 tctgtcttct tctatcattg aggacatcaa cggtattgag actatcaagt ccttgaccag
     1501 tgaaagtcag cgttaccaaa aaattgacaa ggaatttgtg gattatctga agaaatcctt
     1561 tacctatagt cgagcagaga gtcagcaaaa ggctctgaaa aaggttgccc atctcttgct
     1621 taatgtcggc attctctgga tgggggctgt tctggtcatg gatggcaaga tgagtttggg
     1681 gcagttgatt acctataata ccttgctggt ttactttacc aatcctttgg aaaatatcat
     1741 caatctgcaa accaagcttc agacagcgca ggttgccaat aaccgtctaa atgaagtgta
     1801 tctagtagct tctgagtttg aggagaagaa aacagttgag gatttgagct tgatgaaggg
     1861 agatatgacc ttcaagcagg ttcattacaa gtatggctat ggtcgagacg tcttgtcgga
     1921 tatcaattta accgttcccc aagggtctaa ggtggctttt gtggggattt cagggtcagg
     1981 taagacgact ttggccaaga tgatggttaa tttttacgac ccaagtcaag gggagattag
     2041 tctgggtggt gtcaatctca atcagattga taaaaaagcc ctgcgccagt acatcaacta
     2101 tctgcctcaa cagccctatg tctttaacgg aacgattttg gagaatcttc ttttgggagc
     2161 caaggagggg acgacacagg aagatatctt acgggcggtc gaattggcag agattcgaga
     2221 ggatatcgag cgcatgccac tgaattatca gacagaattg acttcggatg gggcagggat
     2281 ttcaggtggt caacgtcaga gaatcgcttt ggcgcgtgct ctcttgacag atgcgccggt
     2341 cttgattttg gatgaggcga ctagcagttt ggatattttg acagagaagc ggattgtcga
     2401 taatctcatg gctttggaca agaccttgat tttcattgct caccgcttga ctattgctga
     2461 gcggacagag aaggtggttg tcttggatca gggcaagatt gtcgaagaag gaaagcatgc
     2521 tgatttgctt gcacagggag gcttttacgc ccatttggtc aatagctaga aagaggagag
     2581 gatgaaacca gaatttttag aaagtgcgga gttttataat cgtcgttacc ataatttttc
     2641 cagtagtgtg attgtaccca tggcccttct gctcgtgttt ttacttggct ttgcaactgt
     2701 tgcagagaag gagatgagtt tgtccactag agctactatc gaacccagtc gtatccttgc
     2761 aaatatccag tcaactagca acaatcgtat tcttgtcaat catttggaag aaaataagct
     2821 ggttaagaag ggggatcttt tggttcaata ccaagaaggg gcagagggtg tccaagcgga
     2881 gtcctatgcc agtcagttgg acatgctaaa ggatcaaaaa aagcaattag agtatctgca
     2941 aaagagcctg caagaagggg agaaccactt tccagaggag gataagtttg gctaccaagc
     3001 cacctttcgc gactacatca gtcaagcagg cagtcttagg gctagtacat cgcaacaaaa
     3061 tgagaccatc gcgtcccaga atgcagcagc tagccaaacc caagccgaaa tcggcaacct
     3121 catcagtcaa acagaggcta aaattcgcga ttaccagaca gctaagtcag ctatggaaac
     3181 aggtgcttcc ttggccagtc agaatctagc ctactctctt taccagtcct acaagtctca
     3241 gggcgaggaa aatccccaaa ctaaggttca ggcagttgca caggttgaag cacagatttc
     3301 tcagttagaa tctagtcttg ctacttaccg tgtccagtat gcaggttcag gtacccagca
     3361 agcctatgcg tcagggttaa gcagtcaatt ggaatccctt aaatcccaac atttggcaaa
     3421 ggttggtcag gaattgaccc ttctagccca gaaaatcttg gaggcagagt caggtaagaa
     3481 ggtacaggga aatcttttag acaaggggaa aattacggcg agtgaggatg gggtgcttca
     3541 tcttaatcct gagaccagtg attctagcat ggttgcagaa ggtgccctac tagcccaact
     3601 ttatccatct ttggaaagag aagggaaagc caaactcaca gcctatctaa gttcaaaaga
     3661 tgtagcaaga atcaaggtcg gtgattctgt tcgctatact acgactcatg atgccgggaa
     3721 tcaacttttc ctagattcaa ctattacaag tattgatgcg acagctacta agactgagaa
     3781 agggaatttc tttaaaatag aggcggagac taatctaact tcggagcagg ctgaaaaact
     3841 taggtacggg gtggaaggcc gcttgcagat gattacgggc aagaaaagtt atctacgtta
     3901 ttatttggat caatttttga acaaagagta atgttcgtgt ttttagagtt aaataatttt
     3961 taaactgtga gaaagattct tcttgcagtt ttttctttac aatttttgaa aaacatctac
     4021 tatttattcg gttaaattct tgtgtttttt ggttttttgt ggtaaaatgt gctcaagtaa
     4081 tacgaaaggc gaactttaaa atgtcaaaac aattgatcta ttcgggaaaa gctaaagata
     4141 tctatacaac tgaggatgaa aatcttatta tttcaactta caaggaccag gcgactgctt
     4201 tcaacggtgt caagaaggag cagattgcag gtaagggagt cttgaataat cagatctcat
     4261 cttttatttt tgagaaatta aatgcggctg gtgtggcgac tcactttgtg gagaaacttt
     4321 cagacacgga acaactcaat aaaaaggtta agattattcc tttggaagtc gtgctccgca
     4381 actatactgc tggttctttt tcaaaacgtt ttggtgtgga tgagggaatc gccttggaga
     4441 ctccgattgt cgaattttac tacaaaaatg atgatttgga tgatccattt atcaatgatg
     4501 agcatgtgaa attcctacag attgcgggtg accagcagat tgcctacttg aaggaagaaa
     4561 cgcgtcgtat caatgaacta ttgaaagtct ggtttgctga gattgggctt aaattgattg
     4621 actttaagct agagttcggt tttgacaagg atggcaagat tatcttggca gacgaatttt
     4681 caccagataa ctgccgcttg tgggacgctg atggcaacca catggataag gatgttttcc
     4741 gtagaggatt gggagaacta accgacgttt atgagattgt ttgggaaaag ttgcaggaat
     4801 tgaaataatc tgtttgcaac ggaaaacctt cgtctctcaa ctaaaaggac tcaggctgaa
     4861 aaggtccccc agaccttttc actctgtaga gaactaggtg aactaacaga tgtttacgaa
     4921 attgtctggg aaaagttgca gggtttaaaa taacaacctc aaggctgttt gggaatattg
     4981 caagagctga aataaaggaa taagaattga tggataaacg tatttttgtt gaaaaaaagg
     5041 ctgattttca ggtcaagtca gagagtttgg ttagagagtt ccagcacaac ttgggactgt
     5101 caagcttgaa aagtattcgt attgtgcaag tatatgatgt atttgacttg gctgaggact
     5161 tgtttgcacc tgcagagaag cacattttct ctgagcaggg aaccgaccat gttttagatg
     5221 aagtatctgt gcaggcggat cttgctaact atgctttctt tgccattgaa agtctgccag
     5281 ggcagtttga ccagcgtgca gcttcgtcac aggaagcctt gcttttgttg ggaagttcga
     5341 gtgacgtgac agtcaacaca gcccaacttt acttggtgaa taaagatatt gatgcgactg
     5401 agttggaagc tgtcaaaaac tacctgctca atccagttga ttctcgtttc aaggatatca
     5461 cgacagggat tgccaagcag gagttttcag agtcagacaa gaccattcca aaattgactt
     5521 tctttgaaag ctatacagca gaagactttg ctcgctacaa ggccgagcaa gggatggcca
     5581 tggaagtgga tgatttgctc tttatccaag actactttaa gtcaatcggg cgcgtgccaa
     5641 ctgagactga actcaaggtt ttggacactt actggtctga ccactgccgt catacgactt
     5701 ttgagacaga gttgaaacac atcgactttt cagcttctaa atttcaaaag caattgcagt
     5761 caacctatga caagtatatt gccatgcgcg aggagttagg tcggtctgaa aaaccacaaa
     5821 ccttgatgga tatggcgact attttcggtc gttatgagcg tgctaacgga cgattggatg
     5881 atatggaagt ctctgacgaa atcaatgcct gctcagttga aattgaagtg gatgttgatg
     5941 gtgtcaagga accttggctc ctcatgttta aaaatgaaac ccacaaccat ccaacagaaa
     6001 ttgagccatt tggtggagcg gctacctgta tcggtggagc tattcgtgat ccgttgtcag
     6061 gccgttccta tgtttaccaa gccatgcgta tttcaggtgc tggtgatatt acagcaccga
     6121 tttcggaaac tcgtgctggg aaattgccac aacaagtcat ttctaaaaca gcagctcatg
     6181 gttattcttc atatggtaac cagattgggc ttgccacaac ctacgttcgt gaatacttcc
     6241 acccaggctt tgtagctaaa cgtatggaac ttggtgccgt tgttggtgcg gctcccaagg
     6301 gcaatgttgt ccgtgaaaaa cctgaagcag gtgatgtgat catccttctc ggaggcaaaa
     6361 caggtcgtga tggtgtcggt ggtgcgacgg gctcttctaa ggttcaaaca gttgagtctg
     6421 tagagactgc tggtgctgag gttcaaaaag gaaatgccat cgaagaacgc aagattcagc
     6481 gcctcttccg taatggcaat gtcactcgtc tgatcaagaa gtccaatgac tttggggcag
     6541 gcggcgtctg tgtggctatc ggtgaattgg cagacggtct tgaaatcgac ctcaacaagg
     6601 tgcctcttaa ataccagggc ttgaatggta cagaaattgc catctctgaa tcacaagaac
     6661 ggatggcggt cgtggttcgt cctgaagatg tggatacctt cgttgccgaa tgtaacaaag
     6721 aaaatattga tgctgttgtg gtggcgacag taactgaaaa accaaatctt gtcatgcact
     6781 ggaatggtga gacaatcgtt gacttggagc gtcgtttcct tgacaccaat ggtgtgcgcg
     6841 tggttgtcga tgccaaagtt gtggacaagg atgtcaaact cccagaagag cgtcaaacat
     6901 ctgctgaaac actggaatca gataccctta cggttctatc tgacctcaac catgcaagtc
     6961 aaaaaggatt acagactatc tttgactgct ctgttggacg ctcaacggtt aatcacccac
     7021 ttggtggtcg ttaccaactc acaccaactg aggcatctgt gcagaaattg ccagttcaac
     7081 acggtgtgac tcatactgcg tcggtcattg ctcaaggttt caacccatat gtagctgaat
     7141 ggtctccata ccacggtgct gcttatgcgg ttatcgaagc aactgctcgt ttggtggctg
     7201 ctggtgccaa ctggtctaag gctcgtttct cttaccaaga atatttcgag cggatggaca
     7261 agcaagcaga gcgtttcggt cagccagtag ctgctcttct aggttctatt gaagcacaaa
     7321 ttcagcttgg cttgccatct atcggtggta aggactccat gtctggtacc tttgaagaat
     7381 tgaccgttcc gccaaccttg gttgcctttg gggtgacgac ggcagatagc cgtaaggtgc
     7441 tctctccaga atttaaagct gttggggaaa atatctacta catcccaggt caagccctct
     7501 ctgcagagat tgattttgac ttgattaaga aaaattttgc tcagtttgaa gcaagccaag
     7561 ctgaccataa agtgacgtct gcatcagctg tcaaatacgg tggtgtagtt gaaagtttgg
     7621 ctcttgctac ctttggaaac tatattggtg cagaggtgac cttgcctgaa cttaaaacag
     7681 ctttgacagc tcaattaggc ggctttgtct tcacatctcc tgaagaaatt gctggagtag
     7741 agaagattgg acaaacgaaa gcagacttta cactgactgt caacggtgtg aagctagatg
     7801 gacacaagct tgacagtgca tttcaaggga cattggaaga agtttaccca acagaattta
     7861 cccaagcgaa agaactagaa gaagtaccag ctgtggcatc agatgttgtg attaaagcca
     7921 aagaaaaggt tgaaaaacct gtggtttaca tcccagtctt tccaggaacc aactcagaat
     7981 atgattcagc taaggccttc gaaaaagaag gtgcagaggt caatttggtg ccattcgtga
     8041 ccttgaatga agaagctatt gtcaagtcag ttgaaactat ggttgacaat atcgacaaga
     8101 ctaatattct cttctttgcc ggtggattct cggctgcgga tgaaccagat ggttcagcta
     8161 agtttatcgt caatatcctg cttaatgaaa aagtgcgtgt ggctattgat agctttatcg
     8221 cccgtggtgg cttgattatc ggtatttgta atggattcca agccttagtc aaatcgggtc
     8281 tcctaccata cggaaacttt gaagatgcta acagtactag tccaaccctc ttctacaatg
     8341 atgccaacca acacgtggcc aagatggtgg aaactcgcat tgccaatacc aactcaccat
     8401 ggttggctgg tgtgcaagtg ggcgatatcc acgctattcc tgtttcgcac ggtgaaggga
     8461 agtttgtcgt gacggctgag gaatttgcag agctccgtga caatggacaa attttcagcc
     8521 aatacgttga ctttaacggt aaaccaagta tggattctaa gtacaatccg aatggttctg
     8581 tccatgccat cgaaggaatt accagcaaga atggtcaaat catcggtaag atgggccact
     8641 cagaacgtta tgaggatggt cttttccaaa atatcccagg caataaagac caacacctgt
     8701 tcgcatcagc ggttaaacat ttcactggaa aataagactt acagattttc taatagatag
     8761 tatcagtaat gtaaaagtca tgtaaatcta gctcttgatg attacaaatg aaaattaggt
     8821 ataaaaaatg acatacgaag taaaatctct taatgaagaa tgtggtgttt tcggtatttg
     8881 gggacatcca gatgctgcta agttgaccta ttttggactc cacagtcttc aacaccgtgg
     8941 tcaggagggg gcaggaatcc tctccaatga tcaaggacaa ctgaagcgcc atcgtgacat
     9001 ggggctttta tcagaagttt tcagaaatcc agccaatttg gataaattga caggagctgg
     9061 tgcgattggg catgtgcgtt atgcgactgc tggcgaagct tctgttgata acatccagcc
     9121 cttcctcttc cgttttcacg atatgcagtt tggcttggct cataatggaa atctgaccaa
     9181 tgcagcctct ctcaagaaag aactggaaca aagaggagca attttcagcg cgacttcgga
     9241 ctctgaaatc ttggctcacc tcattcgtcg tagtcataat cctagcctga tgggcaaaat
     9301 caaggaagcg ctcagccttg tcaaaggtgg ttttgcctat atcttgctgt ttgaggacaa
     9361 gttgattgcg gctcttgacc ctaatggctt tcgtccgctt tctatcggga aaatggccaa
     9421 cggagcggtg gttgtttcct ctgaaacctg tgcttttgag gtcattggtg ccgagtggat
     9481 tcgtgatttg aagccaggtg agattgtgat cattgatgac gagggcattc agtatgacag
     9541 ctatacagat gatactcagt tggcgatttg ttctatggag tatatctact ttgctcgccc
     9601 tgattctaat atccacggtg tcaatgtcca tacggcacgt aagagaatgg gagcgcaatt
     9661 ggcgcgagaa tttaagcatg aggcagatat tgtagttggt gtgcccaatt cttccctaag
     9721 cgcggctatg ggatttgcgg aagaatcagg cttaccaaat gaaatgggtc tgatcaaaaa
     9781 ccaatacacc cagcgaactt ttatccaacc gactcaagaa ttgcgggagc aaggagtgcg
     9841 gatgaaactg tctgctgttt cgggtgttgt caaaggcaaa cgtgtggtca tggtggatga
     9901 ttccattgta cgtggaacaa cctctcgtcg tatcgttcag ctcttgaaag aagcgggtgc
     9961 gactgaggtt cacgttgcca ttggaagtcc tgcactagcg tatccatgtt tctacgggat
    10021 tgatatccag acccgtcagg agctgattgc agccaatcat acggtcgaag aaactcgcca
    10081 aatcattggt gcggacagtc tgacttatct ttcaattgat ggcttgattg agtcgattgg
    10141 tatcgaaaca gatgcgccga acggtggtct ctgtgtcgct tactttgacg gtgactaccc
    10201 aacgcctctc tatgactacg aagaagacta tcgtagaagt ttggaagaaa agaccagttt
    10261 ttacaagtag gcgacagatt ctccattaaa gaaaaggaaa aaacaaatga caaataaaaa
    10321 tgcatatgcc cagtcgggtg tggatgttga agcgggttat gaagttgttg aacggattaa
    10381 aaagcacgtg gcccgtacgg agcgtgcagg tgtcatggga gctcttggtg gctttggtgg
    10441 tatgtttgac ctttccaaga ctggggttaa agaacccgtc ttgatttcag ggactgacgg
    10501 tgtcggaacc aagctcatgt tggctatcaa gtacgacaag cacgatacca tcgggcagga
    10561 ctgtgtggcc atgtgtgtca acgacatcat tgctgcaggt gcggaacccc tctattttct
    10621 cgactacgta gcgacaggga agaatgaacc agctaagcta gaacaagtgg ttgctggtgt
    10681 ggcagaaggt tgtgtgcagg ctggtgctgc cctcatcggt ggggaaacgg ctgaaatgcc
    10741 gggcatgtac ggcgaagacg actatgactt ggctggtttt gcggtcggtg tggctgaaaa
    10801 atctcaaatc attgacggtt caaaggtggt agagggagat gttcttctcg gacttgcttc
    10861 aagtgggatt cactcaaatg gttactcttt ggttcgtcgt gtctttgcgg attacacagg
    10921 tgaggaagtc ctaccagaat tggaaggcaa gaaacttaag gaagttctac ttgagccgac
    10981 tcgtatctat gtcaaggctg tcttgccgct catcaaagaa gagttggtca acggcattgc
    11041 ccacatcaca ggtggtggct ttatcgaaaa tgtccctcgt atgtttgcag atgacctagc
    11101 tgctgaaatt gatgaaagca aagttccagt gcttccaatt ttcaaagccc ttgaaaaata
    11161 tggtcagatt aaacacgaag aaatgtttga aatcttcaat atgggtgtgg gacttatgtt
    11221 ggcggtcagc cctgaaaatg tagagcgtgt aaaagaattg ttggatgaag cagtctatga
    11281 aattggtcgc atcgtcaaga aagaaaacga aagtgtcatt atcaaatgaa aaaaatagcg
    11341 gtttttgcct ctggtaatgg ctcaaatttt caggtgattg ccgaagaatt tccagtggag
    11401 tttgtctttt cagaccatcg tgatgcctat gtgcttgagc gtgcaaagca gctcggcgtt
    11461 ctgtcctatg cttttgaact caaggagttt gagagcaaga cagactacga agcagccctt
    11521 gtcgaactct tggaagaaca ccagattgac ttggtttgcc tagcaggcta catgaaaatc
    11581 gttggaccaa ccttattgtc ggcttatgaa ggtcggattg tcaacattca tccagcctac
    11641 ttgccagaat ttccaggagc tcatgggatt gaggatgctt ggaatgctgg cgtgggtcag
    11701 tctggtgtga ccattcactg ggtggattcg ggtgtggata caggccaggt catcaaacag
    11761 gttcgtgtgc cacgactagc tgatgatacc attgacagat ttgaagctcg catccatgaa
    11821 gcagagtaca ggttgtatcc ggaagtagtg aaggctctat ttacagattg actttttgat
    11881 gattcatatg atatctttga ttttaaattg gagtcagtgt ttgttgaaga cggcttcaaa
    11941 cggaggtatt tgtaatgtta gaatctaaaa aaacaactcg atatgtattt tatgtctatc
    12001 tgatgttatt aacttgggga atcttattta agtttgaaac aaatcctgaa tttatagcat
    12061 ttttcttagc tccaaggtat atcaattgga ttccattttc agaaccacta atagtcgatg
    12121 gaaaaattgt ttttgctgaa atgttattta atctgatttc ctttattcca ttaggtgttt
    12181 gtttcccttt gataaaaact aatttatcta gtttaagaat agtcgggaca ggtttcttga
    12241 ttagtttatt gtttgagtgc ttacagtata ttttagcaat aggtataaca gatataacgg
    12301 atttgacttt aaatacgcta ggtgtctgtg taggcttact gatttatcaa atttttataa
    12361 gagtgttcaa atcacagact agaaaatgga tcaatatctt aggtatgctt agccttggtt
    12421 ttgcttatct tgttttactg ttactgcatt taattggtgt ttaactaatg attaaaaagg
    12481 agaatataat gactaaacgc gtcttaatca gcgtctcaga caaagcgggc attgttgaat
    12541 ttgcccaaga actcaaaaaa cttggttggg agattatctc aacaggtgga actaaggttg
    12601 cccttgataa tgctggggtg gataccattg ctatcgatga tgtgactggt ttcccagaaa
    12661 tgatggacgg tcgtgtgaag accctccacc caaatatcca cggagggctt ctcgctcgtc
    12721 gtgacttgga tagccacttg gaagcggcta aggacaacaa gattgagctc attgaccttg
    12781 tggtggtcaa cctttaccca tttaaggaaa ctatccttaa accagatgtg acttatgctg
    12841 atgcagttga aaatatcgat attggtgggc catctatgct tcgttcagca gcgaaaaatc
    12901 atgccagtgt tacagttgtg gtagatcctg ctgactacgc tgtggttttg gacgaattgg
    12961 cagcaaacgg cgaaacctct tatgaaactc gccaacgttt agcagccaaa gtatttcgtc
    13021 acacagcggc ttatgacgcc ttgattgcag aatacttcac agctcaagtg ggtgaaagca
    13081 agcctgaaaa actcactttg acttatgacc tcaagcaacc aatgcgttac ggtgagaatc
    13141 ctcaacaaga cgcggacttt taccagaaag ctttgcctac agattactct attgcttcag
    13201 ccaaacagct caacgggaaa gaattgtcat ttaataatat ccgtgatgca gatgctgcta
    13261 tccgtatcat ccgtgacttc aaagatagtc caaccgttgt ggctctcaaa cacatgaatc
    13321 catgtggaat tggtcaagct gatgacatcg agactgcttg ggactacgct tatgagtctg
    13381 acccagtatc tatctttggt gggattgtcg tcctcaaccg tgaggtggat gctgcgacag
    13441 ctgagaagat gcacggcgtt ttcctcgaaa tcatcattgc cccaagctat acggatgaag
    13501 cgctagctat tttgatcaat aaaaagaaaa acttgcgtat ccttgccttg ccatttaatg
    13561 ctcaagaggc tagcgaagtg gaagcagaat acacaggtgt agtcggtgga cttctcgtgc
    13621 aaaatcaaga cgtggtcaag gaaagcccag ctgactggca agtggtgacc aaacgtcagc
    13681 caactgagac agaagcgact gctcttgagt tcgcttggaa ggctatcaag tacgtcaaat
    13741 caaatggtat tatcgtgacc aacgaccaca tgacacttgg tgttggtcca ggtcaaacca
    13801 accgtgtggc ttctgttcgc cttgccattg accaagccaa agatcgtctg aacggggcgg
    13861 tccttgcttc agatgccttc ttcccatttt cggataacgt ggaagaaatc gccaaagcag
    13921 gaattaaggc catcatccag cccggtggct ctgtccgtga ccaagaatcc atcgaagcag
    13981 cgaataaata cggcttgact atggtcttta caggtgtgag acattttaga cattaagaag
    14041 ataaaaggga agaaaacagt ttctttcctt ttttggctta aaatgctaac tgaaacaaga
    14101 ttaaaacgaa cttttttgat ataatgttgg taaataattc gcaaaagagg ttgaggaatg
    14161 aaactgcttg ttgtcggttc tggtggtcgt gagcatgcga ttgctaaaaa gttacttgaa
    14221 tcaaaagacg tggaaaaagt ctttgtagct cctgggaatg atgggatgac tctggatggt
    14281 ttggaattgg taaatatctc tatttccgaa cattctaaat tgattgactt cgcaaagacc
    14341 aatgatgttg cttggacctt tatcggtcca gatgatgccc ttgctgctgg tatcgtggat
    14401 gattttaacc aagctgggct taaggccttt ggtccgacta gggctgcagc ggagctggag
    14461 tggtccaagg attttgccaa ggaaatcatg gtcaaatatg gtgtttcgac agcagcctat
    14521 ggcacatttt cagatttcga ggaagccaag gcctatatcg aaaagcatgg tgcgcctatc
    14581 gtagtcaagg cggatggctt ggcgcttggg aagggtgtcg tcgttgctga gacggttgag
    14641 caagcggtcg aagccgctca tgagatgctt ttggacaata aatttggtga ctcaggtgcg
    14701 cgcgtggtta ttgaggaatt ccttgaagga gaggaatttt cactctttgc ctttgtcaat
    14761 ggtgataagt tctacatcat gccaacggct caggaccaca aacgtgccta cgatggcgac
    14821 aaagggccta acacgggtgg tatgggtgcc tatgcgccag ttccacactt accacagagt
    14881 gtagttgata cagcggttga caccattgtc aagccagttc tagaagggat gattaaagaa
    14941 ggtcgccctt atctaggtgt catttacgca gggcttatct tgacagcaga cgggccgaag
    15001 gtcatcgagt tcaactctcg atttggagat cctgaaactc agattatatt gcctcgtttg
    15061 acctctgact ttgctcaaaa tatcacagat atcctggata gcaaggagcc aaatatcatg
    15121 tggacggaca agggtgtgac tctgggtgtg gttgtcgcat ccaagggcta cccgctagac
    15181 tatgaaaggg gcgttgagtt gccagccaag acagaaggcg atgtcatcac ctactatgca
    15241 ggggctaagt ttgcggaaaa tagcagagca ctgctctcaa acggcggacg agtttatatg
    15301 ctggttacca cagtagccac cgtcaaagaa gcccaagcca gcatctacca agaactatcc
    15361 caacaaaaaa tagaaggact cttctaccga acagatatcg gaagcaaggc aattaagtaa
    15421 agatataaga ataacgcgac gcagtcgcca aacacgataa tggtcgtcgt ggtgaaaaga
    15481 ccagaacagt gaatgttctg gtcaggggga aactttgaga ccttaggctc aaagtttagg
    15541 aatgaaaccg aaggtttgct tccgcctcca tcacctaaga ccattatcaa aaagaaaaat
    15601 aaaaattcac aaaatacgtt aatggtcgta tggtttgcga gcgttagcga gcaaatatag
    15661 aataatcacc gccgttgtga aagaacgatt ggatgataat ccaatcgttc agggaaattg
    15721 gaagaccttg ggtttccaat ttaggcatga gacacctttg gtggctgctg ccgtccctca
    15781 caagctaagg tgattgttga aaaagaggaa aaaggagaag aaatgaaacc agtaatttcc
    15841 atcatcatgg gttcaaaatc cgactgggca accatgcaaa aaacagcaga agtcctagac
    15901 cgcttcggtg tagcctacga aaagaaagtt gtttccgcac accgtacacc agacctcatg
    15961 ttcaaacatg cagaagaagc ccgtagtcgt ggcatcaaga tcatcatcgc aggtgctggt
    16021 ggcgcagcgc atttgccagg catggtagct gccaaaacaa cccttccaat cattggtgtg
    16081 ccagtcaagt ctcgtgctct tagtggagtg gattcactct attctatcgt tcagatgccg
    16141 ggtggggtgc ctgttgcgac catggctatc ggtgaagctg gagcgactaa cgcagctatc
    16201 tttgccctcc gtctcctctc tgtagaagat aagtccattg cggatgcact tgccaacttt
    16261 gctgaagaac aaggaaaaat cgcagaggag tcgtcaaatg agctcatcta aaacaatcgg
    16321 aattatcggt ggcggtcaac tgggtcagat gatggccatt tctgctatct acatgggcca
    16381 caaggttatc gcgctggatc ctgcggcgga ttgcccggcc tctcgcgtgg cggaaatcat
    16441 tgtggcacct tataacgatg tggacgccct ccgtcagttg gcagaccgtt gcgatgtcct
    16501 cacttatgag tttgaaaatg tcgacgctga cggtttggat gccgttatca aggatggaca
    16561 actccctcaa ggaacagatc tgctccgcat ttcgcaaaat cgtatttttg aaaaggactt
    16621 tttgtcaaac aaggctcaag tcactgtggc accctacaag gtcgtgactt ctagcctaga
    16681 cttggcagat atcgacttgt cgaaaaacta tgtcctcaag actgcgactg gtggctacga
    16741 tggtcatgga caaaaggtta ttcgttcaga agcagacttg gaagcagcct atgcgctagc
    16801 agactcagca gactgcgtct tggaagaatt tgtcaacttt gaccttgaga tttctgtcat
    16861 cgtgtcagga aatggcaagg aggtgacgtt tttcccagtt caggaaaata tccaccgcaa
    16921 caatatcctg tctaagacca tcgtaccagc ccgcatttct gaaagtctag tagacaaggc
    16981 taaagctatg gcagtgcgaa tcgcagaaca actcaacttg tctggaactc tctgtgtgga
    17041 aatgtttgcg actgatgatg acgttattgt caatgaaatc gccccacgac cacataactc
    17101 tgggcactat tctattgaag cctgtgattt ctctcagttt gacacccata ttctcggtgt
    17161 tctcggagca ccattaccag tcatcaaact ccatgcgcct gccgtcatgc ttaacgttct
    17221 cggccagcat gttgaggccg ctgaaaaata tgtcacagaa aattcaagcg cccacctcca
    17281 catgtatggt aaaatagaag cgaagcacaa ccgcaagatg ggacatgtga ctttgattag
    17341 taatgaacca gatagtgtgg atgagcttgg ggaagggatt gatttttagg acaagtctat
    17401 gatacaaatt atcgttaata catttattga aaaggataag actggagcag ttgttgaagt
    17461 gttgtatgcc agtgctgacc aagataaggt acaagctaaa tatgaagaac tagctgcaca
    17521 ataccccgaa aattatttag ctatctatga tgtaccgctg gatacggatt tgaatacact
    17581 agatcattac ccgtctgtgt ttattggaaa agaggagttt gagtagaaat cttggtttac
    17641 ctagatagct tattcccaac agcttaagaa gaaaggaaaa attaacacat gatcaaccgt
    17701 tactctcgcc ctgagatggc gaatatttgg agtgaagaaa ataaataccg tgcttggctt
    17761 gaggtggaaa tcctctctga cgaggcatgg gctgagttgg gggaaatccc taaggaagat
    17821 gtggctttga ttcgcaagaa ggcggacttt gacatcgacc gtattttgga aattgagcag
    17881 gagacgcgcc acgatgtggt ggctttcacg cgtgcggttt ctgagactct tggtgaagag
    17941 cgcaagtggg ttcactatgg gttaacttct actgacgtgg tggatactgc ttatggttac
    18001 ctctacaagc aggccaacga catcatccgt cgtgaccttg aaaacttcac taatatcatc
    18061 gctgacaagg ccaaggagca caagttcacc atcatgatgg ggcgtactca tggtgtgcac
    18121 gctgagccga caacctttgg tcttaaatta gcaacttggt acagcgaaat gaaacgcaat
    18181 atcgagcgct tcgagcatgc ggctgctggt gtagaagctg gtaagatttc tggtgcggtt
    18241 gggaactttg ccaatatccc accatttgta gaggagtatg tctgcgataa acttggcatc
    18301 cgtgcccaag aaatctctac acaagtcctt cctcgtgacc ttcacgctga gtactttgcg
    18361 gttcttgcca gcattgcgac ttcaatcgaa cgtatggcga ctgagattcg tggtctacaa
    18421 aaatctgagc aacgcgaagt agaagagttc tttgctaaag ggcaaaaagg gtcttcagca
    18481 atgcctcaca aacgcaaccc aatcggttct gaaaatatga ctggtctggc gcgtgtcatt
    18541 cgtggtcaca tgattacggc ttatgaaaac gtcgctctct ggcacgaacg cgatatttct
    18601 cactcatcag ctgagcgtat catcacacca gatacgacca ttttgattga ctacatgctc
    18661 aaccgttttg gaaatatcgt caagaacttg acagtcttcc cagaaaatat gatccgaaac
    18721 atgaactcga cttttggtct tatctttagc caacgggcta tgttgacatt gattgaaaaa
    18781 ggcatgaccc gtgagcaagc ctatgacttg gtgcaaccaa aaacagccta ctcttgggac
    18841 aaccaagtag actttaaacc acttcttgag gcagattcag aagtaacatc acgtctcaca
    18901 caagaagaaa tcgatgaaat cttcaaccca gtttattaca ccaaacgagt ggatgatatc
    18961 tttgaacgtc ttggactagg tgattaatta aaaaataaac agcgagcttc aatctcgctg
    19021 tttatttttt atcgaaaaga cttagtcttc ttttctttta gtgagtccat aggctgctag
    19081 tgtggacatg agtcctgcga ctactagtcc tgcagaatcg tgagttcctg tttcaggaag
    19141 ttttttctct gttaccacag gagctggatc ttgaggaaga gctttgcttt cctcagcagg
    19201 agcagttgat ggagctggtt ggcttgggat ttctagtttt ggtttttctt cagcaatagc
    19261 ggcttgtccg ttttcatcgc ctacatgtgt taccatagtt ccgacttcga ctatttgagt
    19321 aacggcttcc tgtgctacga cactatttac aagtgttttc acttccttac catcggcaga
    19381 agtgctcaca gagtagaagt tgctacgatg tccattgacg cccttagtaa tgacttgtgt
    19441 ttttcctttg agtaagagtg gattttcacg agtcactgtg gtaaatggaa tttcttcttt
    19501 ttggatatcc agtctaggtt ttacctcagt agttggtgca agaccacttt catcaccctt
    19561 gtgagttaca ggagcgccaa cttcaaccac ttggtttata acttctttgg ttacctggct
    19621 atcaaggact gtttctgttg tttttccatt ttcagtgagt acagagatgt aatgagttcg
    19681 ttcacctttg actcctgctg tgataatatt ttcctgacca gctgggaggt taggattttc
    19741 tttcttgata acttcaaatg gaatttcttc agttcttgtg atgagttctg gtctggtttc
    19801 aacattggca gccacttcat tttcatctag gcttcctgaa tgagttgcag ctggtttgag
    19861 gccttgaaga gcggctttta ggttggctac aagtgtgtca agctcagctt gtttattacg
    19921 gttgaggttg taatttagag ctgttttagc tgcgtcaagg gcctcaagac tttctttact
    19981 atatccttct aagtttgtag gaattttagc taattcttcg cggagagcat tataattagc
    20041 acggaagtag tctttgttgt ggtctgcaaa ggcagtcatg agttcaaaga tttcctcttc
    20101 cttgtactca gcgcttggtc tatctgccca gattgaaagc atacttccaa ctgttggaag
    20161 atctacttca ggatatttgg tagaagctag ttgattgaat ggtgtttttc cagtattctc
    20221 aatagctttc ttgaggaaac caccaccatc ttctggtttt tgaccaagaa cataatacca
    20281 gtcaccgttg gtattcaaga acttataacc cttgcttgct aggtattgag gtgatgcgag
    20341 gttgtatccc caccagcctt tagaccagta agaaatcaag acatctttgt caaactgaac
    20401 atcgtccttg tcttcatagt agaagccatc gttgaaggcc attggttgaa gccctctttc
    20461 tttggccata gctgcgaggg tgttggcata ttcggcaaat ttgccataga gttgatacca
    20521 cttgaggtag taccagcctt gggcactagt cgcatcgttg gcgtattcgt cagtaccaaa
    20581 gttgaaaatc tttgttttac ctgcaaagaa gtccatgtat ttaccgatga gggcttttac
    20641 aaagttcatc gcttcttcgt ttttcaagtc catagttgtt tttgaaactt tatcaaagtg
    20701 ggcttgagga tttttaatac ctaatttttc catggcaacc agcatagcat ccatgtgacc
    20761 tggactgtta atagctggga tgagaccgat gtccttagat ttagcgtatt caattagctc
    20821 tgttacttct gcctgtgtta gtgcagtacc gtttggatcg tcgtagtaag ctttagttcc
    20881 ttcgataata gcttttttaa cgtcatcact agcataggtt tttccgttgg cagtaatggt
    20941 catatcatcg agtagaaagc gaagtccgtc atttcctaga aggagatgga catcagaata
    21001 tccgagatca ctggccttgt ctacgatgcg tttgagttgg tccagagtaa agtatttgcg
    21061 tccagcatcg attgagatta ccttgttttt ggcaagtttt tcaacctcac gtttagcttc
    21121 ttcttctttt tgagcttcag gcgtgagggt caagttgttg acagtttctt gaagtttagc
    21181 aatggcttga tcaatcgtat cttgttgggc acggctaagg ttgctatcga gagagcgaat
    21241 agctttttca gcttctttta cggccgtgac gctttctgca gtataacggt tcaggtcttt
    21301 tggtacctcg ttaagtgctt gctctgcaga ttcataatca gctgcgaagt attcagcgtt
    21361 ggcatttgca aaatgacgca tgagtttgaa gaggcgtgat ggtgaataac gtgcagatgg
    21421 agtgtcagtc caagcagcta ccataccacc gatgattggg atatcagctc cttctgtttt
    21481 tggtacagaa gtgattggtg tgtttttaat accattgagc ccctgatcga gattgtacca
    21541 gccttggcca tcagcgtttc gtccaagaac gtagtaccaa gcatcattgg tattaaggat
    21601 ttggtgacct ttttcagcta gtagtttaga agaagcgaca tcgtagcctc cccaaccacc
    21661 agtccacata gaaacgatga tgtctttgtc aaaactacca aagcttgtgt cgctattgta
    21721 gtagataccg tcgttaaagg ccattggttt gagaccgtgc gattttacaa tacgagcgag
    21781 gtcattggcg taggcaataa atttttcata gccttttaca gggtagcctt cgtttggata
    21841 gtatttatca gcttgaagca cactccaacc tttagcatct gtcgcatcat tggcatattc
    21901 atcaagtccg atgttgaaga tttcagtctt tttcgcgaaa taagcagcat acttgtcgat
    21961 aagggctttt gtaaaagcga cagcttgttc gttgtcaaga ttgacagtac gggctgattc
    22021 cttcccaaaa tagctaaagt tagggttttg gattcccaat tctttcatgg cattgagaat
    22081 cgcatccatg tgaccaggac tatttactgt cggaatgaga ccgatacctt tatctttggc
    22141 atagttaatc agatctgtca tttgactttc tgttaagtga ttgccgtttg gatcgttgta
    22201 ataatcattt gtaccttttt caatggcgcg tttgacatcg tcactggcat aggtcttgcc
    22261 gttagctgtg atgctcatat cgtccaacat gaaacggagt ccatcatttc cgactaatag
    22321 gtgtaaatca gtgtagccat aatgtttcgc tttatcgatg atttccttga gctgttctgg
    22381 tgagaaatat ttacgtccag catcaataga aacaattttc tttttcgcta gtttttcatt
    22441 tacagttgca gcacgttcct ttcctgcctc tgtttccggt ttgtcagcct ctgctttcgc
    22501 ttcatctttt ttagctggtt tatccttgtc agtcttgtct gtatttgact ctttagaatc
    22561 aacctctttc gcttcttcct ttttagggct agcttcttct gcctttttat tagcagtttc
    22621 tttttcagca gaagttggag ttaccacctc tgctttatca ctaggagttg aactaacttc
    22681 ctcttgtggt ttttcttctg tttttggaag actagctacc ttatcagtag ctggagtttc
    22741 tgtttctaca gtttttggag cttctggttg aagctctgct ttaggtgttt cctcagtccg
    22801 attttcggat gattgagggg aatcagaaac cgtatggatg gtcggttggt tttctgtagt
    22861 agtaggagta actccatcgg ctgcaacagt ctgtgcttgg aaggcaaatc caattagaac
    22921 agaagctgct cctacagcgt atttacgaat agaaaaacgc tgttgttttt catgtttcat
    22981 tgcaaaacct cctgattgca ttgttatatt gatagcgatt atataaatca acgcctttat
    23041 tttatttctt atattaacga gagtcaagag gagatgacaa aaaactataa taagtataaa
    23101 aaaatataaa atttaaactt aagatttcag attggtcgga aaaaatgaat atatatatct
    23161 agtataattt ttggttctat ttctataaaa tattccacaa attatagaat cttccaaaaa
    23221 taggtaagcg ctaccttttt ggtgtggtat aataagcata gaaaaagccc aagcgattag
    23281 ctcaggcttt cttcttagtg atcacggtca catgagataa atttaatctt gtagtaatca
    23341 gatcgtttgt aagtttcact gtattctaaa acttggccag ttgattcgag tttggtgatt
    23401 ttagtttgta ggacagtagg gaattgttca tcgactccga ggactgaagc cgcatgttct
    23461 ggagttggaa agactatttc gttgatttct tcaaagtgtt catcattcat gtgaatgtgg
    23521 tagtctaact tgaaacgatt atagatagaa ctatagtatt caaggtttgg ataatttgcg
    23581 ttgatatatt gttctgggat gtaggatgta tggtagatat aaacgacacc gtttgattcg
    23641 cggatacgtt caatcttgta gtagaattga tcgccgcgta gacccaattt ttccaagtaa
    23701 acaagcttgt ttccgcgttc aattgaaaga acagttacct tatcatcttt agcattgaag
    23761 agttcaatat ctgaaaactc tacaagcttg tgtttgcgcg cacgtgaaac gaaggttcct
    23821 tttccttgtt ggcggacaat atagccatct ttggcaaggt cgtttaaggc gcgaacaact
    23881 gtgatagagc tgacatcgta cattgaaatg agttctgctt cagtgtaaaa tttatctcca
    23941 ctgctaaact gcccagagat gattttattt tttaattcgt cttttatgta ttgatatttg
    24001 ggaattgcc
//
LOCUS       DAVLYV010000022        24007 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLYV010000022 DAVLYV010000000
VERSION     DAVLYV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.022"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(342..1949)
                     /locus_tag="VYO70_000900"
     CDS             complement(342..1949)
                     /locus_tag="VYO70_000900"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1713156.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYIAFVTAAVENSN"
     gene            complement(2339..2926)
                     /locus_tag="VYO70_000901"
     CDS             complement(2339..2926)
                     /locus_tag="VYO70_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1713157.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(3054..3502)
                     /locus_tag="VYO70_000902"
                     /pseudo
     CDS             complement(3054..3502)
                     /locus_tag="VYO70_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(3507..3896)
                     /locus_tag="VYO70_000903"
     CDS             complement(3507..3896)
                     /locus_tag="VYO70_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1713158.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHFYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3923..4549)
                     /locus_tag="VYO70_000904"
     CDS             complement(3923..4549)
                     /locus_tag="VYO70_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1713159.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRALTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4563..5126)
                     /locus_tag="VYO70_000905"
     CDS             complement(4563..5126)
                     /locus_tag="VYO70_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1713160.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTLAMLVLNYVYAVPLYAKFANFDIGKILGFSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5291..5525)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5579..6082)
                     /locus_tag="VYO70_000906"
     CDS             complement(5579..6082)
                     /locus_tag="VYO70_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1713161.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6275..7105)
                     /locus_tag="VYO70_000907"
     CDS             complement(6275..7105)
                     /locus_tag="VYO70_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1713162.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLATMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQTLKKGDYITLLLATLFLVASLLLILQNQGRFYNPWK
                     "
     gene            complement(7090..8772)
                     /locus_tag="VYO70_000908"
     CDS             complement(7090..8772)
                     /locus_tag="VYO70_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713163.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLSIS
                     KGQIQLQTELVKETPALQSLFILEEVSFSYNDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVNEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8874..9131)
                     /locus_tag="VYO70_000909"
     CDS             complement(8874..9131)
                     /locus_tag="VYO70_000909"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713164.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9138..9686)
                     /locus_tag="VYO70_000910"
     CDS             complement(9138..9686)
                     /locus_tag="VYO70_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1713165.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9701..10549)
                     /locus_tag="VYO70_000911"
     CDS             complement(9701..10549)
                     /locus_tag="VYO70_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1713166.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10737..12086
                     /gene="trkA"
                     /locus_tag="VYO70_000912"
     CDS             10737..12086
                     /gene="trkA"
                     /locus_tag="VYO70_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1713167.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12090..13529
                     /locus_tag="VYO70_000913"
     CDS             12090..13529
                     /locus_tag="VYO70_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1713168.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13656..15335)
                     /locus_tag="VYO70_000914"
     CDS             complement(13656..15335)
                     /locus_tag="VYO70_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1713169.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMMAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGVIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QSISFVFLSLILVVDILIYLPFCRAYDRQLLMKEYVASSNDIILEEETSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLGIQIVATRGMEYIHLTKNPSKSLQFVLEHYQAV"
     gene            complement(15405..16817)
                     /gene="lacG"
                     /locus_tag="VYO70_000915"
     CDS             complement(15405..16817)
                     /gene="lacG"
                     /locus_tag="VYO70_000915"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1713170.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDIALAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSDDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAPLNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            complement(16827..17171)
                     /locus_tag="VYO70_000916"
     CDS             complement(16827..17171)
                     /locus_tag="VYO70_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1713171.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEAYGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            complement(17339..19225)
                     /locus_tag="VYO70_000917"
     CDS             complement(17339..19225)
                     /locus_tag="VYO70_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HEV1713172.1"
                     /translation="MVIRVFDKQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRRDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKQLSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEQFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEISGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGENWQSVVEYLEKL
                     SIYSSCDYFNAIGSRQSDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            complement(19227..20549)
                     /locus_tag="VYO70_000918"
     CDS             complement(19227..20549)
                     /locus_tag="VYO70_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1713173.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMVVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            20717..21940
                     /locus_tag="VYO70_000919"
     CDS             20717..21940
                     /locus_tag="VYO70_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1713174.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            22757..22927
                     /locus_tag="VYO70_000920"
     CDS             22757..22927
                     /locus_tag="VYO70_000920"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1713175.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEKAG"
     gene            23539..23823
                     /locus_tag="VYO70_000921"
     CDS             23539..23823
                     /locus_tag="VYO70_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713176.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            23816..23965
                     /locus_tag="VYO70_000922"
     CDS             23816..23965
                     /locus_tag="VYO70_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713177.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFMAL"
BASE COUNT         7612 a         5197 c         4035 g         7163 t
ORIGIN      
        1 agtatagtgt attgaatcta taacagtcca tcttaactgc taaaatattt atataaatta
       61 atttgacttt cctgatagag atgttcacat cttatttcaa ttcactatac aatccagttc
      121 ctaaaacttt gctagaaatt gatttggctt tccgaattaa tttgttcaga ttttatttca
      181 tttcaatata ctgataaatc cattcactag cacagggcaa gagggagtcg ctattgattt
      241 caacggtagc aggtacatac agaggactcc tacctcagat atactgcaat atcatcgtgt
      301 gcaaaaaacc tctgagattt ttctcaaagg ttctgatttt gctaattgct gttctcaacc
      361 gctgcagtaa caaaggcaat gtagagttct tctggtcggt ttggacggct tgacagttca
      421 gggtgatact gacaagctac aaagaattta ttttcaggaa tttccacgat ttctaccaaa
      481 cgattgtctg gagaaactcc tgaaaagaca aagcctgctg cctcaaactg ctcacggaag
      541 gcattattaa actcataacg gtgacggtga cggcgttgca ccacttcttg attgtgataa
      601 gcagcagcag ccttagagcc acgtttcaac ttagacggat aaagtcctaa acgaagggtt
      661 ccacccatat cctcaatatc aatctgatca cgcatgatat caatgatagg gtattttgtt
      721 tctggtgcaa gctctgcaga attggcacct tcaagaccta aaacgtgacg agcaaactcg
      781 atacatgtca actgcattcc caagcagact cccaacattg gaacatcatt ttcacgcgca
      841 tagcggatgg cttggatttt cccttctgta ccacgttgac caaaaccacc tggtacgatg
      901 atcccgtctg catcagacaa gagttctgct acattctctg ctgtcacatc attggcattg
      961 acccaattga ttttaacttc tgcatcattg acatagccag agtgtttcaa agcttcgacc
     1021 actgagatat aggcatcttg caactccaca tacttaccaa caagggaaat cttaacttgt
     1081 ttcttgaggt tcatgacctt gtccaccatg gctgaccatt ctgtcatatc cgctgctggt
     1141 gcgtctaatt tcaaatgatc acaaacaatt tggtccatcc cttgtgcctg caagttcagt
     1201 ggaatttggt aaaggtgttc aacatccaac gattcgataa cggcttctgg tgccacatca
     1261 cagaactggg ccagtttatt tttaattcct tgaccagctg gctcttctgt acgaataacc
     1321 aacatatttg gttggattcc caagccacgc aattctttga cagagtgttg ggttggtttg
     1381 gttttcattt caccagcagc cttgaggtaa ggaagcaagg ttgtatggat atacatgaca
     1441 ttatccgcac ccacatctgc cttcatctga cgaagagcct ctaggaatgg caaggactcg
     1501 atatctccta ctgttccacc aacctctgtg ataatgacat cagagtcggt cgttagagcg
     1561 gcacgcttga ttttttcttt caaagcatct gtgatatgag gaatgacttg aacagttgcc
     1621 ccaaggtatt ctccacggcg ttctttacga agaacttcac tgtaaatttt cccagttgtc
     1681 acgttggaat atttgttgag attgatatcg atgaaacgtt catagtgacc caagtccaaa
     1741 tctgtctcag ctccgtcatc tgtcacaaaa acttccccgt gctggtaagg actcatggtt
     1801 cccggatcaa tattgatata agggtcaaac ttttgaatgg ttactttgag accacgattt
     1861 ttcaagagac ggcctagact cgctgccaca atccctttcc caatagacga taccacacca
     1921 ccagttacaa aaatatattt cgtagacata gattcctctt tctaaaatgc tcaaggtctt
     1981 gttaactacg aggggatata gaaaacaaga ccctactaat aagaataatc tgggacgact
     2041 gcaccagatt cacaactaat actcttcgaa aatctcttca aaccgcgtca acgtcgcctt
     2101 gccgtagata tgtgttcctg actttgtcag tcttatctac aacctcaaaa cagtgttttg
     2161 agcagcctac ggctagtttc ctagcttgct ctttgatttt cattgagtat aaaatacaag
     2221 aagataactt cttgaaaaaa caaaaatagc tccctaagaa ctagggagcc ccgacctcta
     2281 aaagaggtgc ccaaacaata tgatacctaa aaataggata attgtcaata gctaactctt
     2341 attcctcgct ggtttcagcg tcgtcatctg aaaactcgtc gtcgtcttcg ttgagatcca
     2401 catcttctcc caagtcatct ggagcgattt cgttgatttc tgcatcataa gcttccactt
     2461 catttttttc atcatctgga ttttcatcat cgtatgaaag agctggatct gcttcgtatg
     2521 catcttcgtc ttctggatca tctgcattgt agtcaatggc atctgaatca ccatccataa
     2581 aggcattgac acgttttttc ttagcttttg gtgctacttc atcgtcgtca ttttcttcaa
     2641 gagcgatgat ttcttcgtcg atttcgtcca caccatacca tgaacgaaga ccccatttgt
     2701 tgtccccaag tgagatgaag ctaccgtcaa agttcaactc tgtgtagaac aaaggcaaag
     2761 cttcgcggat atcgctgttt gatgttccaa ggtagttttg aatttcgttt acaagatcgc
     2821 taaaatgcat ctcatgatcg cgaccacgaa gttctaagat agcacgcgct acctcaatca
     2881 tagatagttc acttttttct tgcccagcaa atacttctaa ttccaaagcg tttctcctca
     2941 tttatactac tatcgtcaga gcgaacagac tctgacctca ttttatcatt tactctttat
     3001 tttacgataa ttttgcggaa tagtcaaagg ttaaggggga gaaagtggca ggattagact
     3061 aattccaata taaaacccct tcctttttct gttgctccat tttccacaaa tccaagcgac
     3121 ttgaaacacc tcctagaagc atgattgtag gtgtagattt ccttgactct caattctttc
     3181 catcctttta ctcgagccaa ttcaatcaaa gcacttagaa tctttttcca agtcctcgat
     3241 gttggtaagc ggaattccca atcacaatgg ggagattatc ctgagatagt gtaatatccc
     3301 caattggaaa ccattctccc ttctccttga cttcaatcca aaaaagctca ccatgccgat
     3361 ccaaatagga atacatggct tccaaggtcg cttgactgta aggaagcttc accccatcta
     3421 cgaggtaaac caagttcaca tcctgatacc aagcaaaagc ttcctcacaa tgattgacct
     3481 tataataagg aacaaaacac aatgcactac cttttcaaca aaagagttgc tgcttgatta
     3541 aaaccatcac accagttata ccattttgct tcatacccat cttgagctag gatacgatct
     3601 tctaaatcaa aaacagagta aatctttctt tcctcgcaag cttgcgcata gaaatgatat
     3661 agttcatcac caccatctct atcccactca gcagaaatcg tatcccgacc tgccaataaa
     3721 gcctgataag ccctgtgatg cccatctgta atcagcaaac aatctccaaa ggcaagaata
     3781 ctgattggat cgacttggat tgtttctgcc gactggtaaa gcatctgaat atcttgcaac
     3841 ttcttttctg ataaatatag ttgagtcgga tgaagatctg ctatattgac tttcatttct
     3901 ttctctcaag ggaattcgat actcacttct gtttgccttt aaatcgccat tggaagcgga
     3961 gcttgtcata aaagggaaac tcgataaaca ggactcccaa gcccacacag agactggcaa
     4021 ggacgtctga tgggtaatga actcccagat agactcttga taccagcaca ctgactaggt
     4081 agaggccaag gacgatttgt acgatttttc tccagacctg atctttaatc cgctgactaa
     4141 gaataacaat caaagtccct accatcagcg ttacagctag agaatgccca cttgggaagg
     4201 aaaatccctt ctcttccacc agatgtaaga tagctggtcg tgggcgctgg tagatatttt
     4261 taaaggtcac gattaaaaga cctgccaaag ccagatttcc cagcatgaag aaactttcta
     4321 tcttccatcg cttacgataa aagacaaaag ctgtaatgac aacccaagtg ataatcactg
     4381 ggatatcaat cagacgtgtg agggctcgaa aaagaatagt caaataatct ggtaagtctc
     4441 ctcgaatggc agtctgaatc gattggtcaa aattgaccaa catttcaggg taaaatttga
     4501 ccatatagcc aagaataacg aaaagtaaaa gggcaaaact gcccttcatt aaaaatgttt
     4561 gtttatcttt catagtgttt taaggttggt ttcaaaagga cgtacaacaa ccagaatgaa
     4621 acggaaaaga ttacaccctc aatcaagtta aaaggtaata ccatggtcat taggtagttg
     4681 gaaaatccca aaatttttcc aatatcaaag ttagcaaact tagcgtacaa aggaacagca
     4741 taaacatagt tgagaaccaa catggccaag gttaaaccaa tagttccagc tagagagcct
     4801 agtaggaaac gaagggttgt ccgttccttt ttccaaatca aagcaaatac gatgacaaaa
     4861 actcccaaag ctacgatatt catcggcaaa ccaatgtaag tattcactcc ttggctgtta
     4921 agaagcaatt tcaagagtga gcgaagcaag agcactccta gagcagcagg caaatccatg
     4981 accaccagac ccacaaggac tggcaagata ctaaattcga tcttgaggaa agatgccgct
     5041 ggtaaaagcg gaaagtcaaa gtacatcagc acaaatgaga tggctgatag aattgcaatg
     5101 gtcgaaagtc gacgtgtgtt tgtcataaca ggttcctcca attttctata aaatcagaag
     5161 aagttggaaa ggattcctct atctattctc actttttata tcccaaaagt tccctcttac
     5221 tctattaaag aaaaacaaag caagtggtta caatccggct ataaatctat caaaacagac
     5281 aaagctattc tttcgtcttc tcccatccag actatactgt cggttgtgga atctcaccac
     5341 atcagcttgc gctcgcggac ttctttaaag agaaggaaat agagattttt cttttagact
     5401 aggcgacaag ctgtaggcat aacttctggt taggcaaagc ttgtcaacaa agtataaaag
     5461 gaaaagatca tttcctagtt aaagtcaccg ccggtcggga atcacaccct gccctgaaga
     5521 ctcttttatc ataacaaaaa acgcttgcaa gcgcaagcat tttgttcatt ttcatttttt
     5581 atttcaattt atccacttca taggtatgga cgagctcaag acctgcaaag ttctgctggc
     5641 gaagagcctc atagacaatc atgcagacgg tattagacac attgagactg cggacatgtt
     5701 catcattcat gggaatacgg agagccttct caggatgttc tcgcataaaa tcttcaggta
     5761 agcccttgtc ttcacgtcca aaaagaaaat aatggtcctc gtcagtcgat aaatccacct
     5821 cagaatagac tttttccgcg aatttcgaaa tcagatagag ttttcccttc atctgagata
     5881 tgaaatcttc caaactctcg taaaaataaa tctctagctt atcccagtaa tccaatccag
     5941 cccgcttcat cttgcggtca tcaataggaa agcccatagg cttgatgatg tggaggggag
     6001 aattggtcgc agcgcaagta cgcgcgatat tgcccgtatt ttgtggaatt tgaggttcaa
     6061 ataatacaat gtgatttgtc atgacttgct tcctttcacc attgcaaaaa aatagccaca
     6121 ctgcccggag tcaagctcag cagacagcgt ggttaaggca tcgttaactt acctcacaac
     6181 aggtttgaag taaatcagcg aaactacttt cttagtataa cactttcaga atcattgtca
     6241 atagaaacga cttgattttt tcaatttttt caagctattt ccaagggttg taaaatcgtc
     6301 cctgattctg caagataagt agtaaactag ctactaaaaa caaggttgcc aagagcaagg
     6361 taatatagtc tccttttttc aaggtctgat aactatacca tgtgcgtttt ttctctttcc
     6421 caaagcggcg aagctccatg gcagtcgcaa tggtatcaat gcgttctagc gagctaaaaa
     6481 tcaagggcgt aataatgagc agattgcctt tgattcgttg cataagagaa gctttcttgg
     6541 ataattccat cccacgcgcc tcctgagaca tcttgatagt aaagaattct tcctgcaaat
     6601 ctggaatata gcgcaaggtc aggctgacag aataagcaat cttatagggc acaccaattt
     6661 gatttaaact ggaagcaaac tgactaggat gggttgtcat caaaaagata atagccagag
     6721 gaatggtgca aagatactta atggccaaat ttagcagata aaagagctcc tggctggtta
     6781 gagtgtagac accgattccc tgccaaatca cacttctctc tccataaagt ccaaccccat
     6841 actcgggaga aaagagatag accatcaaaa cgtttaaaac ggcaaatacc gtcgcaaaaa
     6901 cggctacaaa ggaaacatct ttaaagcgaa tttctgataa atagaggaga aagactgaaa
     6961 agatggcaat cagcaagagc attctggtat catagctaat catggtcgcc aatgatacca
     7021 gaatgaaaaa gagaagtttc ccagctcctg acaagcgatg aatcacagta tctctatgct
     7081 ggtaaccgat taatttagct tgcatccctc tctcctctct ttgtaaaatg ccgttaaatc
     7141 caatggatcc acatctagtt tcttagccaa gttaaagatg gaggtttctt ttagattggc
     7201 ttttactaac agctcaggat cgctcaacag actggctgga acagtatcgg caatcaattc
     7261 tccatccacc atgacaaggg ctcgatctga ataatccagc atcaattgca tatcatgggt
     7321 aatcatgaca atggtatgcc cttgttgatg cagttcttcg agaaattcca taatctcagt
     7381 atagttcttc tggtcttgac ccgcagtcgg ttcatctagg aggataattt cagcgcctaa
     7441 gaccaaaatt gaagcaatgg tgacacgttt tttctgacca aatgacaggg cagaaatagg
     7501 ccaattacgg aattcataaa gtccacagat tttcaaggtt tcatagactc tcgtttcaat
     7561 ttccttctca ttcacacctc gcaaacggag ccctagagcc acctcatcaa aaatcatatt
     7621 ggttgaaatc atttgattag gattttgtag cacatagcct actcgttccg cccgctctgc
     7681 aacagaatcg ccttttatat cctgtttttc ccaaagatag cgtccttccg tctgaataaa
     7741 gctacttata gccttggcta gagttgattt ccctgctcca ttttttccga caatagcaat
     7801 cttttcaccc tttttaatat ctaaatgtag ggattttaaa atcggtctat cattataaga
     7861 aaaagatact tcctctagta taaagagtga ctgcaatgct ggggtttctt ttaccagttc
     7921 cgtctgtaac tggatttgcc cttttgagat agacaagtta tccagatctg ctaattgttc
     7981 ttccttgact aagtccacac ctaattgacg gagagtcgtt agataaaggg gttctcgaat
     8041 tccattttga gtcaataaat cagtcgcaag caactggtca gggctcccat taaaaaggat
     8101 acgaccatcg tttatcaaga caatccgatc cacagggcga tgcagaacgt cctccaaacg
     8161 gtgctcgata atcagagtcg tcgtcccctc ttccttatga atctggtcaa tcaattcgat
     8221 aatatcctga cctgacttgg gatctagatt ggcgagtggc tcatcaaaca agagaatcgg
     8281 actttcatca atcaagacac cagccagact gactcgctgc ttttgtccac ctgacaaatc
     8341 ctgaggacgc tgatccagta aaggaagaag gtccagcttt tcagcccatt tataaacacg
     8401 acctttcatc tcatctaggg ctgtcacatc attttccaga gcaaacgcca aatcttctgc
     8461 cacagacaag ccaataaact gcccatctgt atcctgcaaa actgtgctaa ccagatgaga
     8521 cttatcatag atgctcatat caaaggctgt ttgacccttt atcaaaaatt ctccatatgt
     8581 ctgacccttg taaatattgg gaataatccc attcaaacac tgacccaagg tagatttacc
     8641 tgatccagat ggtccaacaa ttaagacttt ctctcccttg taaatggtca agtctatccc
     8701 ttgcaaggtc ggttcttgtt gtgtttcata ccggaaagag aaatccttcc actcaattat
     8761 agcttctttc atcttactct cttcattcgc ttcttagact tctattttat cataaatcaa
     8821 gcccttcttg cagtctcttc tcttaaaatc ttagcgccaa aaagattcct atcctagctt
     8881 acttgcctaa ctaatctata aacatcgaaa aagactagtt gcccagcctt ccccatcatt
     8941 ttatactctt cgaaaatctc ttcaaaccac gtcagcttcg ccttgccgta ggtatggtta
     9001 ctgacttcgt cagtttcatc tacaacctca aaaccatgtt ttgagctgac ttcgtcagtt
     9061 ctatccacaa tttcaaaaca ctgttttgag caacctgcgg ctaacttcct agtttgctct
     9121 ttgattttca ttgagtatta gtcctttttc aaacttcctg cacgagtttg ggttcctgca
     9181 taggcaagta agagaagagt tcctgcaata gctacagata caccattggc aattcccgca
     9241 acaatccctt gtgcaaatac tttttctgcc gcttcttgat aaatcacaac atctccaagt
     9301 ggtgccaaga caccccaaac aagggcattt gcaagtagtt gaatgaggtt aaaaataaga
     9361 atatctttcc agtcaaaaac accattgatc acgcgaacgt actttctaaa aagtcccaca
     9421 actaaaccaa agagtccgct agcgataatc caagtccacc atagaccata accagcaaga
     9481 gagtccttga ttgcatgacc aatcaacccg acaagcaaac cgataatcgg tccaaaaata
     9541 atagaaagta gcgcttgtac cgcatactga agctggatgc ttgtatttgg aacaggggtt
     9601 ggaatgttga tcatcccgat gacgacaaag agggcagcgc caattccgac agcaacaact
     9661 tgtttaattg taaatttaat ttccatacta ttctcctatt ttatccttct attttcttta
     9721 tttcaatggt ccaagatgaa ccgacaccta cattataggc cttggcaaag gaaccttggt
     9781 tgatagccaa acctaaacga tagagagagt tgatgtaaag gatgggttgc ccaattctca
     9841 catctgcaaa tgatttgcca tagacaacct gattttgata gaccagcata tcagcatgat
     9901 agatggtcac ttcaaaacga tcaccaaatt ctggttccag cttgtaaaat tcttcccgtg
     9961 tgatagaggt ccaaagcgaa ccgaaacgca catccagaat atcaatggct cccttcacca
    10021 gatgatcttc tatgatggtc gctacgactg gaagctctac aatctgttcc acactgagct
    10081 ctggccctac ttcctcaaaa gtaatgtgac cactggccag cttagcacca gtataggcat
    10141 agacatcacg accgtggaag gtataagaat gctctgtgtt ttgacgccta ttggccacct
    10201 cagaaatctc acgaatggct acaatgccaa cgtgtttctt gataaaggaa agcgtcccat
    10261 tatctggcgt gacaatgtat tgattttttg cagtcttggc aactacactc ttacgtttcg
    10321 aaccgacacc tggatcgaca accgatacaa acgtcgttcc ctcaggccag taatccaccg
    10381 tctgaaagag acgatagctc ccctcaaaaa tattataagg cgtgatatcg tgcgtcaagt
    10441 gatggatttt taaggttgga gactcttcta aagccactcc aatcatagcc gataccgcac
    10501 catcaaccag accaaagtct gattgtaata ccagtaaatt attattcatt ttatctcctt
    10561 gtatatcctt tatcatacca tatttcagag aaagtcgcta atagctattt caattggtta
    10621 gggtatagtt ttaccttata gcgaattttc actaaaagct cttgttatta gctagtaggt
    10681 gcattttttc ttgaaaaagg gtatgatagt tacatcatga aaaagtaggt tttattatga
    10741 aaattatcct tgtcggaggg ggaaaagttg gttttgccct ctgtcgctcc ttggttgcag
    10801 aaaagcatga tgttttgctg attgagcaag acgaagctgt tctcaatcat attgtcagtc
    10861 gctttgatat cattggtatc cttggtaacg gggccgattt tgccattctt gagcaagcca
    10921 gcgtccaaga ttgtgatatc tttatcgccc tgactgagca cgatgaagtc aacatgattg
    10981 cagcagttct agccaagaaa atgggagcta aagaaactat cgttcgggtg cggaaccctg
    11041 aatattctaa ctcttatttc aaggaaaaga atattctcgg tttttctctt atcgttaatc
    11101 ctgagctctt ggctgcccgc gctatcgcga atatcattga cttccccaac gccctgtctg
    11161 tcgaacgctt tgctggtgga cgcgttagcc tcatggaatt tgtcgtcaag tccaccagcg
    11221 gtctttgcca aatgcccatt tctgattttc gtaaaaaatt tggtaatgtc attgtctgtg
    11281 cgatagagag ggatcatcaa attatcattc caagcggtga catgactgta caggataaag
    11341 atagaatctt tgtcactggt aaccgtgtcg atatgatact cttccataat tattttaaat
    11401 cacgcgccgt gaagagcctt ctcatcgttg gagcaggtag aattgcctat tatctacttg
    11461 gtattctcaa agatagtcgt atcgatacaa aagtcattga aatcaatcct gaaatcgcca
    11521 gcttctttag cgagaaattc ccaaatctct acatcgttca aggagatgga accgcaaaag
    11581 atatcctgct ggaagaaagt gctcaacact atgatgccgt tgcgactcta acaggagtcg
    11641 atgaggaaaa tctgattaca tctatgttcc ttgacagggt aggtgtacag aaaaatatta
    11701 ctaaggtcaa tcgtaccagt ctcctcgaga ttatcaatgc gcctgatttt tcaagtatca
    11761 tcacacctaa aagcatcgct gtagatacga ttatgcactt tattcgtggt cgagttaatg
    11821 cccagtattc agaccttcaa gccatgcacc atctagccaa tggccaaatc gaaaccctgc
    11881 aattccatat caaggaagcc aataaaatga ctgccaaacc tctttctcaa ctgaaattga
    11941 aaaaaggggt tcttattgca gccattattc gaaagggcaa gactattttc ccaactgggg
    12001 aggatatgtt ggaagttgga gacaagctcc tagtaacaac cttgttgcca aacatcacca
    12061 agatttatga cttgattgcg aggtaagaaa tgaataaaag tatgattcgt tacctccttt
    12121 caaagttact tttgattgaa gctgttctcc tcttggttcc tgtggctatc gctgtctatt
    12181 accgtgaatc gagccaagtc tttacagccc tcttttcgac catagggatt ctcgtattgt
    12241 taggcggttc agggagttta cagaagccaa aaaatcaacg gatttatgcc aaggagggag
    12301 tcttgatcgt tgccctctgt tggatccttt ggtctttctt tggtggtctc ccctttgtct
    12361 tttctgggca aatccccagc cttattgatg ccttttttga gattagttcc ggctttacga
    12421 ctactggagc aagtattttg aacgacgttt cagttctcag tcgttccctc ctcttctggc
    12481 gaagttttac ccacttgatt ggagggatgg gagtgcttgt ttttgcactt gctattatgg
    12541 acaatgccaa aaatagccac ctagaggtga tgaaggctga ggttccaggt cctgtttttg
    12601 gcaaggttgt atccaaacta aaaaacactg cccagattct ctatctcctt tatctagctc
    12661 tcttctccct ctttgtcatc atctattatc tagctggtat gcctctctac gatagttttg
    12721 tcattgctat ggggacagcg ggaactggag gctttaccgt ctataacgac ggaattgccc
    12781 actatggcag ctcactgatt acctatctgg tcagtatcgg agttctggtt tttggagtaa
    12841 atttcaacct ctactactac ctcatgctcc gtcgcatcaa ggccttcttt ggagatgaag
    12901 agcttagggc ttacttggtc attgtactgg tttctacagg cttgattagc ctcaacaccc
    12961 tctacctcta cccagggttt tcaaagagct ttgaaatgac cttcttccag gtttccaaca
    13021 tcattacaac aactggtttt ggatatggag atattaccaa ctggcccctc ttctcccagt
    13081 ttatcctcct tttcctcatg gcaatcggtg gctctgctgg atcaactgca ggtggactca
    13141 agattattcg aggcctcatc ctttcaaaaa ttgccaaaaa ccaaattttg tcaatcctat
    13201 cgccccaccg tgttttgacc ctccatgtta ataaaacggt gattgacaaa gatacccagc
    13261 ataagattct caagtacttt gtcatctatg ctatgatttt gctagcgctt atctttattg
    13321 tcagcctaga tagcaatgat tttctggtcg tgaccagcgc tgtctttagc tgtttcaata
    13381 atatcgggcc tattctagga accacttcta gtttctcaat ctttagtccc atctcaaaaa
    13441 ttctcctatc ctttgcaatg attgcagggc gcttggagat ttacccaatc ctccttctct
    13501 ttatgaagag aacttggtct aagagataaa atacaaaatc cctcttttta caattgtaaa
    13561 aagagggatt cttatattaa aaaaggggga acgtttttaa atccaagcct gtctgtaagc
    13621 aaagtcctta ctcaaataaa agatggaaaa acttactaca cagcttggta atgctccaat
    13681 acaaattgta aggatttact tggattcttt gttaaatgaa tatattccat tcctctggta
    13741 gcaactatct gaatacctaa tctttctgca tccaccttca tctctctata ataactccga
    13801 acttgtgggg ccagaataat taaatcataa acacccataa tatcataatg agctccgtac
    13861 gctcctgaat tcgcaatcac tctaacttct gctaattgag cgccctcgtt aattgcattg
    13921 gctaattgcg cacttgttcc agaccctgca caaagaacca gtactttcaa ctccttactt
    13981 tttattacat ctatctcacc aggcattatt tcacttgtct cctcctctaa aataatatca
    14041 tttgagcttg caacatattc tttcatcagt aactgtctat catacgctct acagaatggc
    14101 aaataaatca atatgtcaac aactaaaatc aaagataaaa atacaaagga gatagattga
    14161 aaattcgttc caattaacaa tcccaaggga ccaggaaagg cccaaggtaa ctggatataa
    14221 aatccattca ttccaaagaa atcaataaag acctttccta gaaatacatt cactggtgga
    14281 gtcatcaaaa aaggtacaaa aagataggga ttcaaaataa ctggcatacc aaatagaaga
    14341 ggttcattca ccgcaaataa aacaggagta attgtagctt tacctaccga ttttaattgt
    14401 ttagaccgca taaagaaaat caaaataaat ggtacaacaa aggtagcccc cgttcctccg
    14461 atagcagcga tataatttcc gaaattaggc gttaaagaat gataaggaaa ctgcccattt
    14521 gcaagaagtt gagcattctc ttccatattg ctcagttgta aagctgtaac agcaggtaag
    14581 acaatagatg gtccatgaat tccaacaaac caaagcaaag cgcacataaa ccaaatcaac
    14641 atcatagcag gatatgattc tgccccctta aaaataggag tcaatagttg ttgaaatact
    14701 tgggcaaaag gaacatctaa actaaaccga gatacaatat ctaacaaacc actaataagt
    14761 aaaacaaaag aaaaaggaaa aatatctcta aaagcttgtg atatagcccc aggaacttcc
    14821 ttaggtaaat gaatagtaat gtctcgctta atacagaatc ggtaaacatt tacagtaata
    14881 aaggcactga caaacgaagt tatcaatcct tgagatccta aatagtttgt cgacaaactt
    14941 gtagatcccg ttttctcatc aactacaagc gttacagtta atacaagata gccacatatg
    15001 gccgccaaca ttgcagaagt atcatttatt acctttccag gaggcatttt tctattaaca
    15061 tttccagcca atgactttgc aactgtccct gaaacaataa cacccaaaac tcccatggtt
    15121 agcatataca ctttcctcat ccaggcaata gctggactcg gtaactctac tccgaaattt
    15181 ttaggaatca taataataat catcaaaaag cttgaaaaca taattaaagg catagtagca
    15241 ataaatccat ctttaatagc catcatataa ttattagtag aaattttttc aaatttccct
    15301 tgttgctttt caatggcagc gactaactta tccataaaaa ctcctcctgt aaaaaattta
    15361 agctcatagt taaaatatat taaacatcag tcaaatcgct aaccttaatt ccgttttaat
    15421 ccttttaccc aaagagcact tttcttaata tatctcttct gtgtttcata atcaacaaag
    15481 aaaagaccat atcgcttatt gtagccattc gcccaagaaa attgatcttg caaagaccat
    15541 ataaaatagc cttgaatatt gactccccta tctcgagcct ctaaaacttt gtgtaaatgt
    15601 tgctcaatat aacggattct tttactgtca tcaataatat cattctcgcc ctctggctta
    15661 acctctttaa gggctgtacc attttcagtt aaataaatga ccggatgttg aggatattct
    15721 tctttgatac gaagcaacat atcaaataac ccttgaggat aaatattcca atcccaatct
    15781 gttgtcggaa taccaggctt ttttacaaat tcacctagag catttaattt aaaagaagac
    15841 tgtcctttta ttcctgttga attaaattct tgtctatttt cacctttgta ttcacgaata
    15901 aattctgaac gataataatt catcccaaac atcgtattaa gaggtgcagc tcttttcata
    15961 atttctaaat caccatcttc aataataaag ctagaattat tagcttccaa tatttgattc
    16021 aagtgaaaaa gcgtgtcatc actgtagtag cccaaaaaag ttccatctaa tagaaattta
    16081 ttattataaa catcataccg tttagctgct aaaatatttt ctttttgccc atcaatagga
    16141 tagcctggct ttaaagcatg aatacaacct atctctccct caattcctaa ttgatgaaat
    16201 tcgaggactg caagagcatg cgccaacaac atattatgat tcgcttgaat tgcttcagat
    16261 aattgaaaat gatgatttgg aggaaactga cctcctatat attgacctgc agcaagagac
    16321 atcagttcat tgattgtaaa ccaatgcttg acttctgtaa attcttggaa acaaaagcga
    16381 gcatatcgta tgaaacgatc aatattctct ctattcaacc aatcccctgt ttctaacatt
    16441 ttctgaggcg aatcaaaatg atgtaaagaa acaaacggaa tcacattatg ttttaagcaa
    16501 gactgaaaga ctctatggta ataacgaaca gctaatatat taggctcccc atctatatca
    16561 ggaaatatac gaacccaaga aatagataaa cgcaaagcct gcaaaccatg ttctgccgcc
    16621 aaagctatat cctcttcgta acgataataa aaatcactag ctggatctgg taagaacgga
    16681 ctgttttctt gcaaataaac atcccacata tttgttcctt tgccatctac cctagttgca
    16741 ccctctactt gataagcagc ggtagcagct cctaataaaa actccttcgg taaggcttta
    16801 acctgtaaat tttccatgac taatcctcat tgcaaaccaa tatgttcttc aatctttcga
    16861 attcgttcat attcatcaat aaaaaacttg acctgatcat acaatagcat cgttgtcatc
    16921 aaagtatctt gaccatgaat cataataaag ctcacttcaa aattatctcc atatgcctcc
    16981 tctgctaata aattagtctg ttctcgatgg gcagacacta ttgagtcgtt tgcagactca
    17041 accaattctc ttgcctgttc aaaatatcca tctctcgcaa acgccaaagc atccatcaaa
    17101 tctgaccttg catctcctgc gtaagcgaca attgtaaaac caagcatatt tatttcttct
    17161 tttgatttca tctttcttcc tcctcaatct tgatccatct tttacacatc attattaaaa
    17221 tgacttcgct acgatacaaa tagaaagtcg accctaaata tcttaaaaat ccttaaattt
    17281 cataatattt agggtcaatc ttctctgaat accttagttc attttctata cttatgattt
    17341 ataagtggaa accagcgtaa ttttttgcta cttcaattac acgataaaca tccaaattag
    17401 attgaaatag caattcgtgc ccacggatat aattcaacac atttctccat tcatcttgag
    17461 cttgccttgt ttttccagtt gcttgaagat ataaagcctt cgccatctta acaacatatt
    17521 cacgatgata actgagctgt ttccattcat ccttttgact atttaataac ttagaaatat
    17581 tttcctcaat aattggtaaa aaattatcag ctagattgta agctatataa taatgattcg
    17641 ctaaaacatc actttgacgg ctgccaattg cattaaaata atcacaagag gaataaatgg
    17701 ataatttttc taaatattca acaacagact gccaattttc cccatacaaa gcagaaaagt
    17761 attcttcaat caattcttca taacttcttg tcttcttcca gagcatttcc cccataacat
    17821 aattaggaaa attatgaggg aatccggcac gtaattcttg acacgaaatg taaccgttca
    17881 aatgtaggtt agaaagataa gatacatctc tgtaaatggt ttgactaatt ttcatatagc
    17941 ctaaatcgcc ataatgagca cgccctaaag gatagtcata tacgaaacta tctcctttaa
    18001 atgttttttg ccactcaaaa agataagata aattttcctc aagagaattc ggaagtataa
    18061 ttttattacg cagataaggt ttaggcgtag gtatggaatt gtcaaaatct acatctgcat
    18121 aactcatttc aaatgttctt gtaatcggtg caaacatcat ggtaaagcgt tcaggattat
    18181 ctaatttttc tttctgaggc gcccatagca actcatggta aagcagaaaa caaatctttg
    18241 tatctaatcc ctcactcgtt aaagccctat ccaattgatt gagaatacga atatactgat
    18301 ccgaaaccaa ttcttgtcta cagttttcgc attcacaaat attattacga gcatccgaca
    18361 accatacatg taagtagtta acatcaggtc ttttcttggc ataatccttg ataatttcta
    18421 ccatcttatc cgccacatct ggatttgaaa aatccagact ggttaaaatc ggagccgtat
    18481 taaataattc tcgtttcccg cttatttcag cgacataggg tttcttctcc tctgaaatac
    18541 taagacctga ttcccagcca aattttgaag agtaacctaa aacttcacct gtccatccat
    18601 gaccaacacg atgatgaata agacctcttt tttgcaattc tttatccaac ctatcactca
    18661 attcttgtac taattcattt gaaaattgtt ctttatttag atatggatta aattcatgtt
    18721 cataccaacg tttcaaaaaa gagtagggat tttcaaactg gatgaaaaaa ctgttcatcc
    18781 caatcttagg tagccaatca atgaaatcta gtatattttc aaatgaatcc gctccctcta
    18841 tacatactcc cctatgatag taactggctg tttcatctat agatagctgt ttatctaaaa
    18901 aatcttcaaa tcgtaactct ggaacaaagt catgtccgcg ccctggtcta gtatacacaa
    18961 ccccaaattc atgaaacatt cggtaaattc caataagtaa acctacaatt gtattagact
    19021 caattctccc cttacccttg tctaatgaga taagaacatg gtctcttcta tcctctttat
    19081 tgactaatcc tacaaaaata tccgcttcct tctcctcgac aagttctatg tcagtcttaa
    19141 agacccgatt tatatagtaa cttaattcct ctaaggcaaa gctagaataa gtatttttct
    19201 gcttatcaaa tactctgata accatcttat ttccctccaa cagtagcttt ttctttttca
    19261 agattgcgtt tatctgcaat tttgaagaat ggataataaa ttgctacaga aaccaaaatc
    19321 aaaacaactt gtaatagagc tcctcgccaa ctagctcctg tcgcaaggaa gcctcctatg
    19381 atcggtggca ttgtccacgg aaggattaca cctgttgtat agggtactaa tcctacaacc
    19441 attgatacat aggtaattaa ggcattgatt gtaggagtag caataaacgg aatcagcata
    19501 atcggattta aaactgttgg aaacgtaaat agaatagctg tattgatatt aaaaatagac
    19561 ggtataatag ctagcttacc taatgtttta ttcgcacgac tcttactaaa taggaagaga
    19621 caaatcgcaa gaccaatagt cgctccaccg ccaccaataa atacaaataa atctttaaac
    19681 ggtaatgtaa taatgtgttg gagtgtttgt cctgcagcca cagcttcttg gttttctgta
    19741 gtaaattgta accaaactgg atctacaaaa gcatttaaaa cttgtccccc attaactcca
    19801 cagaaccaaa agaatgagtt tacaataaca catagaatca tacctggaag cgttcctgca
    19861 attaacttaa gcggtgttcc aacaattgca cctaggagtc cgttgagacc tcctgcaact
    19921 cctgccgctt caagaccttt taagaccaga gcccacaaaa caaaagtagt aaagccaggt
    19981 aaaagagctg aaaatgattt acttaccaca tctgggacac cacttggcat ttttatcgta
    20041 attccgcgct ggataaacat acgataaatc tctgctgtaa ccaatccaat agtaatcgcc
    20101 ataaacaaag aagatgcatt caggctagaa aatggtattg cgccgccaaa taactgcttg
    20161 acctgctccc catttttatc ataaaccata ctcgaaaaac gaggtgccat caatacaaaa
    20221 ctggataagg ctatgatccc tgccgacgga ccatctgtac catatccttc cgacaacctg
    20281 taggcaatac cgaaacaagc gaccaaggag ataatcgcta ctgttgactg attcatagtt
    20341 gtcaagatac ttagcaatcc aacactattc agccaatttg taaaatcctc ttgaggccaa
    20401 ctgattagaa taagaaataa agagccgact aaaatcaaag gcattgccat cataaatcct
    20461 tcacgaatag ctttcaaatg ccgttggttg ccaacttttt caccaaacgg agcgactttt
    20521 gtttctaaaa acgctaaaat attgttcatc accggaactc ctttttttat tttttataaa
    20581 ttaaatatac cactaacaga aagcgttgtc aatatatttt ttgttgtttt tacaatattt
    20641 ttttctttgt atagaataat aattttaatt gattttttct tatgtttaat ataaaataaa
    20701 ccaaaggagg aaacaaatga ttacaaaaca atcagccttt ttacaaaata gagccactat
    20761 actggagttt ctatatcgaa atccagcaac atcccgtaca gatattgtta atgaaacagg
    20821 ccttactcca gcaacaacga ctaacatcat aaaagagcta agcgaacagt ccctcatata
    20881 cgaaactggt gatgaattta gcgagttttc cggatctgga agacgtagaa aaactatttc
    20941 tatcacagat aatattccgt atgttgttgg aggtattgaa ataaacgtcc taggtatctt
    21001 tctcagtcta tgtgacttac aagggaaaac tcttttcgag acagaaattt tgaatgaaga
    21061 ttatcctatt tcagaaatca attccaccat taccaatatg ataaaaacag ctatagagta
    21121 cgtccctttg gaaacaaaat tacttggatt tggcttatca atacctggac attataacaa
    21181 agactccgga agtatcatta caaacaaccc catatgggaa tcttttaatt tattaaatgt
    21241 aattaaaaga ttcaattttc cttttattgt aaaaaataat atcgattgta tggctatagg
    21301 acaatacctt tttaatccac acaatacccc cgataacttt attttcctac acgctggatt
    21361 aggtatttac acttcctttt tcacaaaaga aaaaatagga gcctctaaaa atccttatat
    21421 cggagaaatt ggacacacca ttgtcgaatt gaatgggcaa tattgtgaat gcggaaaaaa
    21481 aggttgttta caaacatata tttcggatgc ttggttaatc aaacacgccc aattattatt
    21541 taaaaattcc caactaactg tactaaaaag ccttgtaaag actgaaaaag acattcattt
    21601 agacaccctt ttaacggctt ataatttagg cgactccgct ttacgccaac aaattgataa
    21661 aggagtcaat ttattggcca cttctattgc aaatcttctc ctcatcaatc ctgctgataa
    21721 aatctatatc aacagtcaat tgcttaatta tcaacctttc actcatgaag tcagggataa
    21781 aatccaagac cagctccact tcgttccctt tactcgtaat atagaaattg aaattttacc
    21841 ttacaacaaa catcgtggaa gtataggagc ttgtgcatta gctatcgttg cttttttcat
    21901 agagcatagc aatgtattac aagatattat ttcaccttaa tatatattag aaatctatac
    21961 acctgtttaa atcaactata acctgtagta gatatctcgt atttagacaa tatgaaaaca
    22021 agacgacttc catataggaa accgccttct cgctatgttg agtgatttat attaaaataa
    22081 cttttcttct agctgcattt tattattata aaaacattca tcataacccc cagaacttaa
    22141 ataacaattt ttattcaaga tacatactcc tagaataaac tttatatgaa atccccattt
    22201 ttgtttttac aattctccgt atactcaatg aaaatcaaag agcaaactag gaagctagct
    22261 gcaggctgct caaaacactg ttttgagctt gtagatagaa ctgacgaagt cagtaacatc
    22321 tatacggtaa ggcgacgctg acgtggtttg aagagatttt cgaaaagtat tagttaaatc
    22381 ttgtttaata tctctacacc atactatttt acatatatta tttagcgcca catagtactg
    22441 aactctctcc aaaaacggtt atgcctcttt gaatagggcg ttatcacaag aaaagcatct
    22501 ccacgtttca acttcatatg gctcaaaaac aatcaattga tgctaaaacc tgtacctaga
    22561 tgtttcggtt cataaaacca tgaaactgta aaagtggatg aaattgatag cgatagtcaa
    22621 atcaagaggc atcataactc taaaaagtca caatatataa gttcatcctc ggaaaaatat
    22681 cattctaatt gttgaaatgc ctacatgaaa agaaacgtca aatgctcatg aaacaacgaa
    22741 tacaggtatc aaaactatga caaaacaagt ccctaaattt actaaagaca ctgctcaact
    22801 ttacacctgt aaatggttgt tgtataataa agttacaaag atgtacgacc acactgttgt
    22861 aaatcatagt gttcgcgaat atattactga tagcatttct acaaatacaa ttgaaaaggc
    22921 tggataggct ttaaacgaaa tatgtcagtg ttttggcatt cctagccttc atatcattta
    22981 aagaattgta tagacaaaat ttttcccaat acagacactc gtaacaactg cttcattttt
    23041 ctaccaacat atttaggaac aggataagat acaagaggat taatccatag atcagttcta
    23101 taccaatcta agacaaataa gctaaaaaaa cgattgataa taagcaaata gattccaaat
    23161 tttctctatc tgctcatttt aataaacaat actagtgtaa ctatccttcc agtcagaagc
    23221 ttgtcaaatc acaccgaaaa ttcttctaaa atttatctcg ttaggcaatc aagcaaaaac
    23281 tcgacgatag tacaaacatt atcatacaga attgacttcc taaattatat actttagtaa
    23341 tgttttcgga taagaaaaaa gattcatttt acatttctaa acattctttt ctaagatgaa
    23401 aaacagaatt tttcgattgt gatttaaagc aacaagaaga ttttcagtat catcctatag
    23461 atacgagcta attaagaaaa actacatttt cgaatataaa ctacaataat ataaactaaa
    23521 ttttatagga ggaagacaat ggattggtac gattatatga tacaggcatc caaacaatca
    23581 caattcaacg caagccattg gtttcgctat ttgcgaaaag ttatttttga agactattct
    23641 tatttaacaa accaagatgt agaaaagttg ctagactcca aagaactaac ccgttttcaa
    23701 aaaattagct tgaagtatgc ctttcaagag catactccaa ctcataaata tgtgatttca
    23761 ttaaataaac ctgctaagtt aaccaatgtt caaaaattga tggagaaata caaacatgga
    23821 taaaatgaaa ccggtcttcc aagccctaaa taaggaatta attcaggaaa atctgacttt
    23881 aacaattatc tgtgtcgatg gttatgtctt agaatatcat ggtttacgtg ccacacaaga
    23941 tgttgatgct tttatggctc tataatattt gtagtgggta aatcccctat ggatattatg
    24001 gagccta
//
LOCUS       DAVLYV010000023        23953 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLYV010000023 DAVLYV010000000
VERSION     DAVLYV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.023"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(105..2363)
                     /locus_tag="VYO70_000923"
     CDS             complement(105..2363)
                     /locus_tag="VYO70_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1713178.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKVLKAVMTSKGNIQIKSA
                     KKTEVKSSEKEK"
     gene            complement(2592..2822)
                     /locus_tag="VYO70_000924"
     CDS             complement(2592..2822)
                     /locus_tag="VYO70_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1713179.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3012..3695
                     /locus_tag="VYO70_000925"
     CDS             3012..3695
                     /locus_tag="VYO70_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1713180.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3695..4429
                     /locus_tag="VYO70_000926"
     CDS             3695..4429
                     /locus_tag="VYO70_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1713181.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4583..5440
                     /locus_tag="VYO70_000927"
     CDS             4583..5440
                     /locus_tag="VYO70_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1713182.1"
                     /translation="MTQIIDGKALAVKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASYITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5582..6454
                     /locus_tag="VYO70_000928"
     CDS             5582..6454
                     /locus_tag="VYO70_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1713183.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     RDDASGENLVKQVFVNLSQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            6736..7419
                     /gene="rpiA"
                     /locus_tag="VYO70_000929"
     CDS             6736..7419
                     /gene="rpiA"
                     /locus_tag="VYO70_000929"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1713184.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7433..8644
                     /locus_tag="VYO70_000930"
     CDS             7433..8644
                     /locus_tag="VYO70_000930"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1713185.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8646..9203
                     /locus_tag="VYO70_000931"
                     /pseudo
     CDS             8646..9203
                     /locus_tag="VYO70_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            9203..10012
                     /locus_tag="VYO70_000932"
     CDS             9203..10012
                     /locus_tag="VYO70_000932"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1713186.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10723..11472
                     /locus_tag="VYO70_000933"
     CDS             10723..11472
                     /locus_tag="VYO70_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1713187.1"
                     /translation="MTGHFLGCYLAQIAAVEAYQKYPDFYNHVLRRVTTFSAPKVITS
                     RTVWNAKNGFWDVGLESRKLAVSGKIKHYVVDNDNVVTPLIHNDRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATFDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11495..12010
                     /locus_tag="VYO70_000934"
     CDS             11495..12010
                     /locus_tag="VYO70_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1713188.1"
                     /translation="MCQHKLRLTIKKARYVRIELEGYNALSLAEVEVFRFIATNGETA
                     IQVSKSVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNG
                     RNQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTT
                     LNSKVSDDDVL"
     gene            12664..13374
                     /gene="deoD"
                     /locus_tag="VYO70_000935"
     CDS             12664..13374
                     /gene="deoD"
                     /locus_tag="VYO70_000935"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1713189.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKFGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13397..13921)
                     /locus_tag="VYO70_000936"
     CDS             complement(13397..13921)
                     /locus_tag="VYO70_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1713190.1"
                     /translation="MKITIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFLPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13974..14210)
                     /locus_tag="VYO70_000937"
     CDS             complement(13974..14210)
                     /locus_tag="VYO70_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1713191.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14278..15198)
                     /locus_tag="VYO70_000938"
     CDS             complement(14278..15198)
                     /locus_tag="VYO70_000938"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1713192.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVEAFAHQVWTSINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15396..15800
                     /locus_tag="VYO70_000939"
     CDS             15396..15800
                     /locus_tag="VYO70_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713193.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGICFVETYYGFTFLM
                     EHAIVTAVFIYSFSIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16016..16597
                     /locus_tag="VYO70_000940"
     CDS             16016..16597
                     /locus_tag="VYO70_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1713194.1"
                     /translation="MYYAENPDAAHDIHELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16594..17871
                     /locus_tag="VYO70_000941"
     CDS             16594..17871
                     /locus_tag="VYO70_000941"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1713195.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLEDGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17889..18551
                     /gene="deoC"
                     /locus_tag="VYO70_000942"
     CDS             17889..18551
                     /gene="deoC"
                     /locus_tag="VYO70_000942"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1713196.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18538..18927
                     /locus_tag="VYO70_000943"
     CDS             18538..18927
                     /locus_tag="VYO70_000943"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1713197.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19018..20070
                     /locus_tag="VYO70_000944"
     CDS             19018..20070
                     /locus_tag="VYO70_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1713198.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20214..21749
                     /locus_tag="VYO70_000945"
     CDS             20214..21749
                     /locus_tag="VYO70_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713199.1"
                     /translation="MAHENVIEMRDITKVFGGFIANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIHRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21742..22800
                     /locus_tag="VYO70_000946"
     CDS             21742..22800
                     /locus_tag="VYO70_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713200.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22803..23759
                     /locus_tag="VYO70_000947"
     CDS             22803..23759
                     /locus_tag="VYO70_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713201.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
     gene            23844..>23953
                     /locus_tag="VYO70_000948"
     CDS             23844..>23953
                     /locus_tag="VYO70_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1713202.1"
                     /translation="MKLSYDDKVQIYELRKQGYSLEKLSNKFGINNSNLR"
BASE COUNT         6982 a         4303 c         5437 g         7231 t
ORIGIN      
        1 actatatatc ataatggaca aagggcaagc tgtcaagaaa ttacagttat tttagtaaaa
       61 aaagaagcag aaaaatttca ttttctactc cttttctata ggatttattt ttctttttca
      121 gaacttttaa cttcagtttt tttggcagat ttaatctgaa tgtttccctt gctagtcata
      181 actgctttga gaactttttc gcttggattt tcaaggtagt agtcagtgat tgtgtcctca
      241 atatagtctt gaatagttcg acgaagtggg cgtgctccca tttttggatc ataacctagg
      301 tcaaccaact tttccttgac cttgtcagtt acatccaaac gaatgttgtt gctagagagg
      361 cgcttgttaa catctgctag catgagctcg acaatctgaa ggaggttatc cttgctgaga
      421 gccttaaatt cgataatgcc atcaaaacgg ttcataaact ctgggctaaa gaagttaccg
      481 agttcaccga ggacagaatt ggtacgtcct tctctagcag caccaaatcc aacgctagct
      541 tcggtctttc ctgtacctgc atttgaggtc atgataatga tggcatcctt gaagctaacg
      601 gtgcgtcctt gcccgtctgt caaacgacca tcgtccaaga cttgaagaaa catgtgcata
      661 acatctgggt gagctttttc cacttcatcg agaaggatga gagaatatgg attgtggcga
      721 actttttcag ttaattgacc agcctcatca tagccaacat aacctggagg agcgccgacc
      781 aacttagcca cactatgttt ttccatgtat tcactcatat caaagcgaat catactatca
      841 gcagaaccaa aaagttcgat agccagttgt ttggaaagtt ctgtcttacc gacaccagtt
      901 ggcccaacga agaggaagct tccgattggg cggttagggg taccaagtcc gacacgatta
      961 cggcgaatag ccttggcaat cttatcgact gcatcatctt gaccaataac atgagacttg
     1021 agatcttcgg ctagatggat gagttgagat tgttctttct ctttcaaatc accaacaggg
     1081 atattggttt tctgctcgat aatgtgctca atagttttct cgctgatgat aggagtatcc
     1141 tggtctgtga tctttttctt ttgcatttcc ttatacttgg caatctggtc gcggaagtag
     1201 gccgccttct caaaatcttc ttctcgtgta gcttgagact tgagattttc agcctcaatc
     1261 aagcgctgat caattacttt aggatccaca aaattcaagg tcaagttcat cttagaacca
     1321 gcttcatcta ggaggtcaat ggccttgtca ggcaagaagc gatcttggat gtagcgattg
     1381 gaaagagttg cagctgcttc aatcgcagca tctgtatatt gaacgtggtg gtaatcttcg
     1441 tatttctttt gaatcccttt gagaatagtg attgtttcgt ccaccgttgg ttcatcgact
     1501 ttaacaggct gcatacgacg ctcgagggca gcatcctttt caatgatacg gtattcattg
     1561 agggtagtag caccgactag ttgcagttct ccacgagcaa gggctggctt gaggatattt
     1621 cctgcgtcca tattaccatc actcgcagaa ccagcaccaa caatttcatg gatttcatcg
     1681 ataaagagga tgatgtcttc acgtttgcga atttcttcca tgagtttttg catgcgttct
     1741 tcaaattgtc ctcgaatccc cgttccttga actaagctaa ccacatccag acggatgact
     1801 tgtttacctt ggagtttatg tggcacatcg ccatcgacaa ttttctgagc tagaccttcg
     1861 acaacggccg tttttccgac accaggttca ccgataagga caggattatt cttggttcta
     1921 cgattgagaa tctcgatgac acggataatc tcatcgtcgc gcccaataac ggggtcaatg
     1981 tctccacgac gggcaatttc agttacatta ataccaaatt cttccagcag gcctttttct
     2041 tggctaggtg gcggagtttg agcagatcca cgattttggg aaccataacc gccgtttcca
     2101 ccgtaacctc cacctgattg ggttggggga ataggaggag tattgctaga aggtctgaaa
     2161 ttgtttagat cattgaagaa atcaccaaag ggatcgaagt cacgattgtt cagatccgtc
     2221 atacctttga agaggctatt gttaggatct gttttgataa tcttatagca gttttgacag
     2281 aggtcaattt gtttttgttt tccattgaga ttggtgtaaa gatgaattgt tgagtcgtta
     2341 attttacagt tttgacaaag catacatcta cctcatttct ttctttagcc tgacttgttt
     2401 aaaggtcaaa aatagtcaaa ttctatactc tcattataac aggattgggg tgtaaagcaa
     2461 gtaaaaagat tgaagctttg agaagttgtt acaatgaaaa tttttgtgaa tttggactat
     2521 aaaaaaatcc tatttgtgag acaaaaagga ttttacttag acatgcaggg acaatcccag
     2581 cttgttttag attagtagag aagttcgtaa atttccattg cgattaagtc aatgctatca
     2641 aacgtatacg tttcgcgatc ttctacgtca cgaagggtaa agattgggcc gttttcttcg
     2701 tcgtggctgt acgcaacttc tgcaacaaca actccttcgc gttcaaaatt acgttttaaa
     2761 tttccgccat cgtttgccat tgcttcaagg cggttgatga ttctaaccaa atgtgattcc
     2821 attttgattc tccttcattt cttatcgtta ctattttacc ataaaggagg ccaacttgac
     2881 tagaaaaaac taagatttct cactaattct gctagtttaa agatttttaa ataaattgga
     2941 taaaagattt aaattttaat tcaaaacatg ttataatcat acagtattct attttagaaa
     3001 gcagtgtgac tatgaatttt tcttttttac ctaagtattt accttatttt aactatgggg
     3061 ctgttgtgac gattcttatt tctatctgtg ttatcttttt gggaactatt ttgggtgttg
     3121 tcttggcttt tgggcaacgt tcaaagttta aaccgcttgt ttggttggcc aacttgtacg
     3181 tttggatttt ccgtgggaca ccgatgatgg ttcaaattat gattgccttt gctcttatgc
     3241 atatcaatgc tccgactatt cagattggaa ttttaggtgt tgatttttcg cgtctgattc
     3301 cagggatttt gattatctct atgaatagtg gtgcttatgt ttcggagact gttcgtgccg
     3361 gaatcaatgc ggttccaaaa ggtcagctag aggcggctta ttcgctaggg attcgtccta
     3421 aaaatgcgat gcgttatgtg attttgccac aagcaatcaa aaatatcttg ccagcattgg
     3481 ggaacgaatt tatcaccatt atcaaggaca gctccctctt atcagctatt ggggtcatgg
     3541 agttgtggaa tggggctaca acagtttcta caacaaccta tctaccttta acaccacttt
     3601 tatttgcagc attttactac ttgattatga cctctattct gacagtagcc ttgaaagctt
     3661 ttgaaaaaca tatgggacaa ggagataaga aataatgaca gaaaccttga taaaaattga
     3721 aaatttacat aaatcttttg gaaagaatga agtattgaag ggaatcaacc tcgagattaa
     3781 aagaggagaa gttgtcgtta tcatcggtcc ttcagggagt gggaaatcta ccttgcttcg
     3841 ctctatgaat ttgttggagg aagcaaccaa ggggaagctt atctttgagg gagtcgatat
     3901 tacggacaag aagaatgact tgtttgccat gcgtgagaag atgggcatgg ttttccaaca
     3961 atttaacctc tttcctaata tgactgtgat ggaaaatatt accttgtcac ctatcaagac
     4021 taaaggtgaa agcagggaag ttgctgagaa gagagcccaa gagcttttgg aaaaagttgg
     4081 tttgccagat aaggcagacg cttatccaca gagtctgtcg ggtgggcaac aacaacggat
     4141 tgccatcgcg cgtgggttgg ctatggaacc agatgttttg ctctttgacg aaccaacttc
     4201 agccttggat cctgaaatgg taggtgaggt attggctgtt atgcaagacc ttgccaagtc
     4261 agggatgact atggttatcg taacacatga gatgggattt gcccgtgagg tggcagatcg
     4321 tgttatcttt atggcagacg gtgtggttgt tgaagatggg acacctgagc agatttttga
     4381 acaaacccaa gaacaacgga ctaaagattt cttgagtaag gttttataat ctattttaaa
     4441 atttcaagac aagattagtg aaaagttcaa ccagagcttt ttcttatagt ttgaaactat
     4501 aggataacct aggaaagaag tgttagaggg cactagcagt ttttggtata atggaagata
     4561 tttgaaagaa aagagaagtg atatgacaca gattattgat gggaaagctt tagcggtcaa
     4621 attgcagggg cagttggctg aaaagactgc aaaattaaag gaagaaacag ctctagtgcc
     4681 tggtttggta gtgattttgg ttggggataa tccagccagc caagtctacg ttcgcaacaa
     4741 ggagaggtca gctcttgcgg ctggttcccg tagcgaagta gtgcgagttc cagagaccat
     4801 tactcaagag gaattgttag acctgattgc taaatataat caggatccag cttggcatgg
     4861 gattttggtc cagttgccat taccaaaaca tatcgatgaa gaggcggttt tattagccat
     4921 tgacccagaa aaggatgtgg atggtttcca tcccttaaac atgggacgtc tttggtctgg
     4981 acatccagtc atgattccct cgacacctgc aggaattatg gaaatgtttc atgaatatgg
     5041 gattgacttg gaaggtaaaa atgcggtcgt catcggtcgt tcaaatatcg ttggaaaacc
     5101 tatggctcag cttcttttgg ctaaaaatgc gacagtaacc ttgacccact cacgaactca
     5161 taatcttgcc aaggtggctg ctaaagcaga tattcttgta gtcgcaatcg gccgcgccaa
     5221 gtttgtgact gctgactttg tcaaaccagg tgcggttgtc attgacgttg ggatgaaccg
     5281 agatgaaaat gggaagctct gtggagatgt tgattatgag gcagttgcac cacttgctag
     5341 ctatatcacg ccagtccctg gaggtgtcgg tcctatgacc attaccatgc tgatggagca
     5401 aacttatcaa gcagcacttc ggacattgga tagaaaataa gagaaacatt cgattaatag
     5461 agtgtatttt ctatagctat atctaaaatg gcagaaatga atattaaatt ttagatataa
     5521 gattacaaaa ggaggtctgc gtctcctttt tattgtataa taaaagtgag aggaaaaaag
     5581 gatgaaagtg attaatcaaa ccttattaga aaaagttatt attgaacgtt ctcgttcaag
     5641 tcataaagga gattatggtc gtctgctgct gcttggtggt acttatcctt atggaggtgc
     5701 catcatcatg gctgctttag cagctgtaaa aagcggtgca ggattggtga ccgttggaac
     5761 ggacagggaa aatatcccgg ctctgcacag ccatttacct gagcctatgg ccttttctct
     5821 tcaagaccag caattgttaa aagagcaatt ggagaaggca gaagttgtct tgttggggcc
     5881 tggtttacga gacgatgctt ctggagaaaa tctagtaaaa caggtctttg ttaatttaag
     5941 ccaaaatcag attttgattg tagacggagg tgccttgacc attcttgcta ggacaagttt
     6001 gtcatttccc tcaagccagc ttatcctaac tcctcaccaa aaagaatggg aaaaattatc
     6061 tggaattacg attgaaaagc aaaaggaaga tgcaacggct agtgttctga cttccttccc
     6121 tcaaggaaca attttggtgg agaaaggccc agctactcgt atttgggaag ttggccaatc
     6181 tgattattac cagttacagg ttggtggtcc ttatcaggcg actgggggaa tgggagatac
     6241 tctggctggg atgatcgcag gatttgtagg ccaattccga caggccagtc tctacgagcg
     6301 tgtggcagta gcgacccatc ttcattcagc catagcccaa gaactatctc aagaaaatta
     6361 tgtggtctta ccgacggaaa tcagcaggta tcttcccaaa ataatgaaaa ttatttgtca
     6421 acaagagagg gtaagcaaag ataaacttgt ttaaaataga cagataaatc tacttccttc
     6481 tcttaattta tgattcttga aatgcgcctg ctttaaggct aaactagacg tatcaaagtt
     6541 aagagtttgt ttttgtaact ggaaagagat gaacaaatca aatacataaa agataaactc
     6601 atttttattc gtttggtaaa aataaaatgc atatttttaa agaaagattg atgaaaaatg
     6661 tagatttgat gtttttactt gaataaaaag cggtttcata gtaaaataag aactgagaat
     6721 gatggaggta aatcggtgga aaatctgaag aaaatggcag gtatcaaggc tgctgagttc
     6781 gtgagcgatg gaatggtcgt tggacttgga acaggctcga ctgcctatta ttttgtcgaa
     6841 gaaatcggtc gtcgtatcaa ggaagaagga ttgcagatta cagctgtaac aacttctagt
     6901 gtgaccacta aacaggcaga aggccttaat atcccgctca agtctattga ccaagtagac
     6961 tttgtcgatg tgacggttga tggcgcggat gaagtggata gtcagtttaa tggaatcaaa
     7021 ggcggtggtg gtgcccttct catggaaaag gtggtcgcaa caccatcaaa agaatacatt
     7081 tgggtggtgg atgaaagcaa gctggtcgaa aaactaggtg cttttaaatt gccagtagaa
     7141 gtggttcagt atggtgcaga gcaggtcttt cgtcattttg aacgagctgg ctacaaacca
     7201 agtttccgtg aaaaagacgg ccaacgtttt gtgacggata tgcagaattt tatcattgac
     7261 ctcgccttgg atgtcattga aaatccaatt gcttttggac aagaattgga ccatgtcgtt
     7321 ggtgttgtgg agcatggttt attcaaccaa atggtggata aggtaatcgt tgctggacga
     7381 gatggagttc agatttcaac ttcaaaaaaa ggaaaataga agggggcata agatgtctaa
     7441 atttaatcgt attcatttgg tggtactgga ttctgtagga atcggtgcag caccagatgc
     7501 taataacttt gtcaatgcag gggttccaga tggagcttct gacacactgg gacacatttc
     7561 aaaaacagtt ggtttgaatg tcccaaacat ggctaaaata ggtcttggaa atattcctcg
     7621 tgaaactcct cttaagactg tagcagctga aatcaatcca actggatatg caacaaaatt
     7681 agaggaagta tctcttggta aggatactat gactggacac tgggaaatca tgggactcaa
     7741 cattactgag cctttcgata ctttctggaa cggattccca gaagaaatcc tgacaaaaat
     7801 cgaagaattc tcaggacgca aggttattcg tgaagccaac aaaccttatt caggaacggc
     7861 tgttatcgat gattttggac cacgtcagat ggaaactgga gagttgatta tctatacttc
     7921 agctgaccct gttttgcaga ttgctgccca cgaagacatt attcctttgg atgaattgta
     7981 ccgtatctgt gaatacgctc gttcgattac ccttgagcgt cctgcccttc ttggtcgcat
     8041 cattgctcgc ccttatgtag gtgaaccagg taacttcact cgtacggcaa accgtcgtga
     8101 cttggctgta tctccatttt ccccaactgt tttggataaa ttgaatgagg ctggtatcga
     8161 tacttatgct gtgggtaaaa tcaacgatat ctttaacggt gctggtatca accatgacat
     8221 gggtcacaac aagtcaaata gtcatggaat tgatacacta ttgaagacta tgggacttgc
     8281 tgagtttgaa aaaggattct cattcacaaa cctagttgac tttgatgccc tttacggcca
     8341 tcgtcgtaat gctcacggtt accgtgattg tttgcatgag tttgatgaac gcttacctga
     8401 aattatcgca gctatgagag agaatgacct tctcttgatt actgcggacc atggaaatga
     8461 cccaacgtat gcagggacgg atcacactcg ggaatatatt ccattgttgg catatagccc
     8521 tgcctttaaa ggaaatggtc tcattccagt aggacatttt gcagatattt cagcgactgt
     8581 tgccgataac tttggtgtgg aaactgctat gattggggaa agtttcttag ataaattggt
     8641 ataagatgac gcgctatgct ttgctggtga gaggtatcaa tgttggtagt aagaataagg
     8701 tcgttatggc ggagcttcgt caagaattga caaacttggg actggaaaag gttgagagct
     8761 acatcaacag tggcaatatt ttctttactt cgatagattc caaagcccaa ttggtttaaa
     8821 agctagagac tttctttgca gtccattatc catttattca gagcttttct ttactgagtc
     8881 tagaggactt tgaggcggaa cttgaaaatc taccagcttg gtggagcaga gacttggcac
     8941 gaaaagattt tctcttttac actgagggtt tggatgtgga ccaagtcatc gcgacagttg
     9001 aaagtttaga gctgaaagat gaagtgcttt attttagaaa acttgggatt ttctggggga
     9061 aattttctga agaatcctat tctaagactg cctatcataa gtacttgctg aaggtgcctt
     9121 tctaccgcca cattactatt cgtaatgcta aaacctttga caaaattggt caaatgctaa
     9181 aaaataataa aggagacaca taatgacatt tttaaacaaa atccatgaaa ctgctacttt
     9241 cctgaaagaa aagggaattg cagcccctga gttcggtcta atccttgggt caggacttgg
     9301 agaattggca gaagaaatcg aaaatccagt tgtagtagac tatgctgaga ttccaaactg
     9361 gggccgttca acagtagtcg gtcatgctgg taaattggta tatggtgaac tggcaggtcg
     9421 caaggtcttg gctcttcaag gtcgtttcca tttctatgaa gggaatcctc tggaagtggt
     9481 gactttccca gttcgtgtga tgaaagttct tggatgtgaa ggtgttattg taaccaatgc
     9541 agctggcggt atcggatttg gtcctggtac cttgatggct atctcagacc atatcaacat
     9601 gacggggcaa aatccattga tgggtgaaaa cttggatgac tttggcccac gtttcccaga
     9661 tatgtctagg gcctacacac cagaataccg tgccactgcc catgaagtgg ctaaaaaact
     9721 taatatcaag cttgatgaag gtgtctatat cggagttact ggtccgactt atgaaacacc
     9781 agcagaaatt cgttcctata agacactggg agcagatgca gttggtatgt ctacggttcc
     9841 tgaagttatc gtggcagccc actctggctt gaaagttcta ggaatttcat gtatcactaa
     9901 ctttgcggct ggtttccaag aagaactcaa tcacgaagaa gttgtagaag tgactgaacg
     9961 tgttaaaggt gatttcaaag gcttgcttaa agcgattctt gctgaattgt aagaaaaaag
    10021 atttaaaagg ggaagtacct ctgttttttc aggattgact gcctatccgg attaaagaag
    10081 aaacagagga atactatgag cttcttcctg ctcttataac tgaaagaagc ggaagaatag
    10141 gtatgtctga tctgatagcc agcattgtga aagacaagat tctaggatac tagcattagc
    10201 ttcctagcca agcagactag tatgataagg agagatgaga atgaattgac tttctgaatt
    10261 tctcagtctt atcatatata gcacaatgag atttcgcttg agtctgcttg taaataaacg
    10321 aaaagaaaga taagaaataa tgaaaattgg tcaacgaatt atgcgctttg gcataaaaaa
    10381 ttaagtatcg gagttgtatc tgttgtagtc ggctttgatt tctagctcca gctggaattt
    10441 cagccaatga agtaaagtaa gatgtaacat ctgaagtggt aataggtgtg ttagattcta
    10501 aggaggaatt gaaagagtca gaaaatgatg ctctaaaact agaaactcct cttagagagg
    10561 agccaagact agctcctcaa acgcttccgg aagcaagtga agttagttct tgaaaacaaa
    10621 agggaagagt caaaagtaga gataacagaa ccagctcaag cggatgatat ccgcaaggtt
    10681 gttggggaat tagccaagga tataagtatt actaagttgt atatgacagg tcattttctt
    10741 ggatgttacc tagctcagat tgcagcggtt gaagcttacc aaaaatatcc tgatttttat
    10801 aaccatgtat tgaggagagt gacaactttc agtgctccta aagtgattac ttccagaact
    10861 gtttggaatg ctaagaatgg tttctgggat gttggtttgg aaagtcgtaa attagctgtt
    10921 agtggaaaaa ttaagcatta tgtggttgat aatgacaatg ttgtgactcc cttgattcat
    10981 aatgatcgtg atattgttac atttacaggt aattcacgct ttaaacaccg ttctcgtggc
    11041 tattttgaaa gtccaatgaa tgatattcct aactttaata ttggtaaaca agctaccttt
    11101 gataaacatg gttatcgtga tccgaaattg gataaagtgc gattctttaa gaaacaggct
    11161 ctacctcaat cttctagtca accaagcgct gaaccaatgg aaaatattgc cttaggaaaa
    11221 caggttactc aaagttcgac agctttcgga ggagatgcta gaagagctgt ggatggcaaa
    11281 gtcgatggta actatggtca caattctgtc actcatacaa acttccaatc taagccttgg
    11341 tggcaagtag atttggctaa agaagaaacc attcgccaaa tcaatattta caaccgaaca
    11401 gacactgccc aggatagatt ggcaaacttt gatgtcattc ttttagacag ttctggtaaa
    11461 gaaattgagt gaaaacgtat aacatctcct aaagatgtgt cagcacaaat tacgattaac
    11521 cataaaaaaa gcgcgctatg ttcggattga gctagaaggc tataatgccc tcagtcttgc
    11581 agaagttgaa gttttccgct ttatagctac gaatggtgaa acggcgatac aagtttctaa
    11641 gtcagttcaa ccaatcagtc agactcctgt gaaagataaa acattgacaa ttcaacacag
    11701 tggagcttac attgcccgct actccataac ttgggaagaa gttacagtag ataaagatgg
    11761 aaaccaagtt gttcgtagtc attcttggga aggaaacggt cgcaaccaga ctgcaggttt
    11821 tgtcctcaac ctcccaatca aagaaaatat gagaaatctg cgagttaaga ttgagaaaaa
    11881 gacgggccta ctatggaata gatggcaaac aatctatgaa aacagaccaa ttttagctca
    11941 accccaccgt aaaattaccc attggggtac gacattgaat tccaaggtga gtgacgatga
    12001 tgtcttgtaa tctgatggta gaatgacagt tagtttgtct agtttataag aaagtactac
    12061 ctgagcttga atagaactca ggtagctctc tatgaaagaa caaaattaat actcaatgaa
    12121 aatcaaagag caaactagga aactagccgc aggttgctca aagcactact ttgaggttgt
    12181 agataagact gacgaagtca gtcacatata taatccaagg cgacgttgac gtggtttgaa
    12241 gagattttag aagagtataa acagaaaggt agagcgcgtg ttctaatttg aacacgagta
    12301 gaaaactttt ctaaaaacaa aaacgaaagg atgggtaaac tgtattcgct gaactgaata
    12361 cgggcgactc tcctctaaat caaaattaag aaaggaattg accccaccct aaaagtagtg
    12421 ggaaaaagat agttggtcta gcgagcatcg ctcactgcgc ccaactccta ttttcccttc
    12481 gctttttgat gggtttggta tctttctcaa tataaaatat aaaataaaga aaggtagagc
    12541 gcgtgttcta atttgaacac gagcggaaaa ctcggaaaat agataatctg actgaaaaat
    12601 caggatttct cgtcaggttc ctaattttca gtcgttttct tctcgctctt tgtatcataa
    12661 attatgtcta tccatattgc tgctcagcag ggtgaaattg ctgataaaat tcttcttcct
    12721 ggggatcctc ttcgtgctaa gtttattgcg gagaatttcc ttgatgatgc tgtttgtttt
    12781 aacgaagtgc gtaacatgtt tggttacact ggtacttaca agggtcactg tgtatctgtc
    12841 atgggaactg ggatgggaat gccatctatt tcgatttatg cgcgtgagtt aatcgtagac
    12901 tacggtgtga agaaattgat tcgtgtggga actgcaggtt ctttgaatga agaggttcat
    12961 gttcgtgaat tagttttggc gcaggcggct gcaaccaact caaacatcgt tcgtaatgac
    13021 tggccacagt acgattttcc acaaattgct agctttgatt tgcttgataa agcctaccat
    13081 atcgccaaaa aatttggtat gactactcac gttgggaacg ttttgtcatc tgatgtcttt
    13141 tactcaaatt actttgaaaa gaatatcgag cttggtaaat ggggagtcaa ggctgtggaa
    13201 atggaagcag cagctcttta ctatcttgct gcccaatacc atgttgatgc gctagctatc
    13261 atgaccatct ctgatagctt ggtcaatcca gacgaagaca caactgcaga agaacgtcaa
    13321 aataccttca ctgatatgat gaaggttggt ttggaaacct tgattgcaga ataattatag
    13381 ccaaaaagga agccctttat gaattgtagg acttcctttt cttatccaga aattgatcta
    13441 gttctctctg atttcgaaga atagtgactt tatgtgaata ttcttggcaa agtttttggt
    13501 aattttcttt ttgagttttg ctacgcccat cccaaagaat ccatctgata aactcccact
    13561 caaagcgttc agggcaatct gccgccatac tttctctgac ttttccacgg tatttaagat
    13621 aacgcttaaa ggctctaaag agacaggtca atggcaaaaa attgagaaag atgatttggt
    13681 cagcttcttg cattcgttct tggtagtagc accaagaata attaccatcg atgacccaag
    13741 ctttatgctt ggtgagaaag ttttttatct cggttaacat ccattcgcag tcactgtctt
    13801 gccaaccagg ttgaaattgg agtgtgtcca tgtgcagttt tggaatggag tagtagttag
    13861 ataacttttc tgctagagtt gacttaccag aaccagaata tccgataatt gtgattttca
    13921 ttttctacct tttcctattt ggagacaaaa aaacagcctc tatggactgt ttcttattta
    13981 gcaagtttag ctgaaagacg agctttatcg cggcttgctt tgtttttgtg aatcaaacct
    14041 ttagtttctg ctttatcgat agctgagcta gcagcacgga aaagttcttc agatgggttt
    14101 gcttcaaaag cttttatagc agtacgcata gctgattttt gagctgagtt cttttcgttt
    14161 tgtttaacgt tcaattcagc gcgtttgata gctgatttaa tgtttgccaa tgttcttacc
    14221 tccatattta ctaactatac cattatatat gaaaacttac gttttgacaa gggaaaatta
    14281 tttttttaag taaatttcat cgatttcatg gttcttgctt ttatgaagaa tcacttctgc
    14341 acgatttctg gttggttcaa tataattttg tagatttgtg agattgatac tggtccagac
    14401 ctgatgggca aaggcttcca cttccccaat cggcatctga gtaaaacgat aatagtagct
    14461 atcagggtcg ttttgggcta gactcagcat tttcaagaaa cggtccagat accaactttc
    14521 aatatcatcc actccagcat ctacatagat ggaaaagtca aagaagtcag tgatatagag
    14581 acgatcgttt tgtggatttt gaaagacatt gattccctca acaattacaa aatcagcagc
    14641 tttgacactt tgtttctctt cgggtacgat gtcgtaaact tcatgagaat agacaggaat
    14701 atctacatct tgtccatttt tgatgcggtc caagaagttg agaagagctt ccatatcata
    14761 gctttcagga aatcctttac gatttaaaat cccctgctca atcaaggttt gattgggata
    14821 gagaaaacca tcagttgtaa ccaactcaac cgtagcatct gtaaacgtac gggacagtag
    14881 gatttgaagt aggcgactgg ttgtggattt tccaacggca acactcccag aaaccccaat
    14941 aataaaaggt tgagatttac tttcacgttg gaggaaaatt ccttttgaaa aggctaaatc
    15001 ttccttagtt cgcttgtaaa tctgaatcag atgagccaag gggagataga tatctgtaac
    15061 gtcttggaga ctgatttggt cattaaaact cttgatagat tccaattctt cttctgtcaa
    15121 aggaggtgtt gtctttcgat gtaaagattg ccaagtctgg cggctgattt tttcaaaatg
    15181 taaaaattcg ttggtcattt gttttcccct agatattttg tctatcatac cataaaaatc
    15241 taaaaaaata aagcggtttc ttgaatgtgg taagtcaatg gtatatagta taagtgatca
    15301 atttattgat gaaataggag atagattcta ctgatttata agtttataag cttataaagg
    15361 gggcttttta gttcacaact tataggaggt gtactatgaa aatcttaaaa cgttacatat
    15421 tggaactctg ttttatttta agttttgctt taccttttat aaaaggagcc aatgcagata
    15481 atggtatatg ctttgtggaa acctattacg gttttacttt tttgatggaa catgctattg
    15541 taacagctgt ctttatctat tcgttttcaa ttgctttctt actaaaaaaa cgatgggcga
    15601 aatggattgc tgctggtagt tattgctttt tagtcctatg gattgcgaca gagggttatt
    15661 tcttccgcat gtcactagaa gatttgatac gactttggac aagtttggaa atcctgacac
    15721 aaacgtatca gttgggcttt tatctaaaca ttttgttggg aattttgttg ataataaagt
    15781 attttaaggt taagcaatag aaataataat ctgcctgatt atagatgata aactttttgt
    15841 ttttatctgg actgtgatat ttatagttct aaatggttta ttatttataa gtgttgaaag
    15901 agattgaaga aaatggcttc gtgactcgct ttcttcggtc tttttcgttt tattatcaat
    15961 ttgtaaacga tttacaattt gagtcaaatt atggtaaaat agtgtcatga gtaaaatgta
    16021 ttatgcagaa aatcctgacg ctgctcacga cattcatgag ttgagagtag agttgttggg
    16081 acaaaaaatg acctttttaa cggatgcggg tgtttttagc aagaaaatag ttgactttgg
    16141 aagtcaacta ttgctcaagt gtcttgaggt caaccaagga gaaactgtcc ttgatgtagg
    16201 ctgtggttac ggacctttgg gtttgtcctt agctaaggct tatggagttc aggctactat
    16261 ggttgatatc aacactcgtg ccttagattt agctcggaga aatgctgaaa aaaataatgc
    16321 aaaagcgacg attttccagt ccaacatcta tgaacaagtt gaaggacatt ttgatcatgt
    16381 catttccaat cctcctatcc gtgcgggcaa gcaagtcgtt catgaaatca ttgagaaaag
    16441 taaagatttc ttggaaactg gtggggattt gacaatcgtc atccagaaaa aacaaggggc
    16501 tccaagtgct aagtccaaga tggaggatgt gtttggtaat tgtgaaatcc ttaaaaaaga
    16561 caaaggatac tatatcctta gaagtgtgaa agaatgagag cagttgattt aatacaaaaa
    16621 aaacgagacg gtcaagaact gacttcaagt gaaattgaat ggctggtaga aggctatgtg
    16681 tcaggaactg ttcctgatta tcagatgtct gcctttgcta tggctgttta ttttaaagga
    16741 atgacgactc gtgaaatttc tgatttaacg atgaatatgg ttaagactgg gcaagagttt
    16801 gacctgtcag ccattgatgg tgtgaaagtt gacaagcatt ctactggtgg tgtcggcgat
    16861 aaggtaacct tgattttagc tcctcttgtt gctagcttcg gtgtgcctgt agcaaaaatg
    16921 agtggtcgtg gtcttggcca tacaggcgga acaattgata aattggagtc cattaggggc
    16981 tatcaagtag aacgtagtca agaggatttt attcgtcagg ttcaggatat tggagtatct
    17041 gtcattgggc aatcagacca gctggtcaaa gcagataagc ttctctatgc ccttcgtgat
    17101 gtgaccgcaa ctgtcgacac gattcctttg attgcgagtt cggtgatgag caagaaaatc
    17161 gcggcagggg cggatgctat tttgctagac gtgactgtcg gtgagggtgc cttcatgaag
    17221 acggttgatg aggcgcgtga gttggctcaa actatggtag atcttggtaa ggttgttgga
    17281 cggaagacag tagcagtgat tacggatatg agccagcctt tgggacgagc gattggaaat
    17341 cgtctggaaa tccttgaagc attggagatt ttgcaaggtc aaggacgtca ggatattacc
    17401 cactttattt gtgaattggc tcaaattatg cttggcttgg caaatgttaa caagactgtt
    17461 gaagaagttc gccaacatct tgaggatggt caagcactgg ctaagtttga ggaaatggtg
    17521 caagcccaag gtggagactt ggaagacctc tatcgtcctg taaatgtagc tcatgtggtg
    17581 gaaatccctg ctcaggaaac aggtgtcatt tcagctcttc cagctatgga ctttggtctt
    17641 tatgccatga ggctgggtgc tggtcgtgca gttaagtctg atgccttgga ctatgaaaca
    17701 ggaattgttt ttgaaaagaa gattggagac tccgttcaaa agggtgaaat tgttgcaaaa
    17761 gtatacacaa atgaaaaaat tccacctcaa ctagttacag atttccaaaa atgtgttaaa
    17821 ataagtgatg aagtgaaaaa aatccgtgaa attgtagaaa tcatctccta aatggaggga
    17881 aaaccgaaat gaaattaaat aaatatatag atcatacgct tttaaaacaa gatgcaaaga
    17941 aaaaacaaat tgatagtttg ttgtctgagg ctagagagta tggctttgcc agtgtttgcg
    18001 ttaatccaac ctgggttgaa catgctaaaa aagggcttga aggcacagat gttaaggttt
    18061 gcacggtagt aggtttccct ttgggagcaa caacttcagc cgtgaaagca tttgagacaa
    18121 aagaagctat ccaaaatggt gcagatgaga ttgatatggt gatcaatgtt ggagctctca
    18181 aatcaggtaa tttagccttg gttgagtcag atattcgcgc agtagtggaa gcaagtggtg
    18241 ataagttagt gaaagtcatt attgaagctt gccttctgac agaccaagaa aaagttgttg
    18301 tttgccaatt ggcccaaaaa gctggggctg actttgtcaa aacatctact ggcttttcaa
    18361 ctggtggtgc tacgatagca gatgttacat taatgcgtga aacagttgga tctgatatgg
    18421 gagtcaaggc tgctggtgga gctcgttctt atgcagatgc tcttgccttt gtcgaagcag
    18481 gtgcgacccg tatcggaacg tcagctgggg tagctatttt aaaaggagaa ttggcagatg
    18541 gcgactactg agttgattga actggcaatt gaaaccagca aacatgccta tgtcccctat
    18601 tctcactttc ctatcggagc agttttagta gccaaagacg gaaatgttta cacgggagtg
    18661 aatatcgaaa atgctagcta tcctttgacc aattgcggtg agagaacagc tatttttaag
    18721 gctatatctg aaggccaaag agaattttca gaattgattg tctatggtca aactgaaaaa
    18781 ccgatttcac catgtggtgc ttgtcgccaa gtaatggtcg aattttttga acaagattta
    18841 aaagtgacct tagtcgcaaa agataaatcg acggtcgaga tgacggtcgg ggagttactt
    18901 ccatattctt ttacagactt aaattagtct gagtcgctct ttgagtggca agggtctttg
    18961 tgaccaatca atccatactt gcaacatcgt tgcacatctt atttaggagg ttcagtaatg
    19021 aacaagaaac aatggctagg tcttggccta gttgcagtgg cagcagttgg acttgctgca
    19081 tgtggtaacc gctcttctcg taacgcagct tcatcttctg atgtgaagac aaaagcagca
    19141 atcgtcactg atactggtgg tgttgatgac aaatcattca accaatcagc ttgggaaggt
    19201 ttgcaggctt ggggtaaaga acacaatctt tcaaaagata acggtttcac ttacttccaa
    19261 tcaacaagtg aagctgacta cgctaacaac ttgcaacaag cggctggaag ttacaaccta
    19321 atctttggtg ttggttttgc ccttcacaat gcagttgaag aagcagctaa agaacacact
    19381 gacttgaact atgtcttgat tgatgatgtg attaaagacc aaaagaatgt tgcgagcgta
    19441 actttcgctg ataatgagtc aggttacctt gcaggtgtgg ctgcagcaaa aacaactaag
    19501 acaaaacaag ttggttttgt aggtggtatc gaatctgaag ttatctctcg ttttgaagca
    19561 ggattcaagg ctggtgttgc gtcagtagac ccatctatca aagtccaagt tgactacgct
    19621 ggttcatttg gtgatgcggc taaaggtaaa acaattgcag ccgcacaata cgcagccggt
    19681 gcagatattg tttaccaagt agctggtggt acaggtgcag gtgtctttgc agaggcaaaa
    19741 tctctcaacg aaagccgtcc tgaaaatgaa aaagtttggg ttatcggtgt tgaccgtgac
    19801 caagaagcag aaggtaaata cacttctaaa gatggcaaag aatcaaactt tgttcttgta
    19861 tctactttga aacaagttgg tacaactgta aaagatattt ctaacaaggc agaaaaagga
    19921 gaattccctg gcggtcaagt gatcgtttac tcattgaagg ataaaggggt tgacttggca
    19981 gtaacaaacc tttcagaaga aggtaaaaaa gctgtcgaag atgcaaaagc taaaatcctt
    20041 gatggaagcg taaaagttcc tgaaaaataa tggatggaag tcatttctta ggaagcggcc
    20101 aagggccgct tttttaagag ttgagacaaa gtcattttgt ctcagtaaag cttgctacta
    20161 gacaaactag caagttttat tgaaataaaa taagactctg aaaggaagag cacatggcac
    20221 acgaaaatgt cattgagatg cgtgatatta ccaaggtgtt tggtggattt attgccaacg
    20281 acaaaatcaa cttgcaccta cgaaaaggtg aaattcatgc acttttagga gaaaatgggg
    20341 ctggtaagtc cacgctaatg aacatgttag caggccttct tgaaccaact agtggtgaaa
    20401 tcgcggtcaa cggtcaagtt gtcaatctcg actccccatc taaagcagct agcttgggaa
    20461 tcgggatggt tcaccagcac tttatgttgg ttgaagcctt cacagtggct gaaaacatca
    20521 ttttaggtag tgaattgact aaaaatggtg tgctagatat cgctggagct agcaaagaaa
    20581 tcaaggctct ttctgaacgt tatggcttag ctgttgaccc ttctgccaag gtagcagata
    20641 tctcagttgg agcccaacaa cgtgtagaaa ttttaaaaac actttatagg ggggctgata
    20701 tccttatctt tgacgaacca acggctgttt tgactccatc agaaattgat gagttgatgg
    20761 ctattatgaa aaatcttgtc aaagaaggaa aatcaattat cttgattacc cacaaattgg
    20821 atgaaattcg cgcagtttct gaccgtgtta cagttatcca tcgtgggaaa tcaattgaaa
    20881 ccgttgaaat tgcaggggct accaatgctg atttggcgga aatgatggta ggacgttctg
    20941 tttcctttaa aacagagaag caagcctcta aaccaaaaga agtggttttg tctatcaaag
    21001 atttggtggt caatgaaaac cgtggtgttc cagctgttaa aaatctgtcc ttggatgttc
    21061 gtgctggaga gattgttggt attgcgggga ttgatggaaa tggtcagtct gaactgattc
    21121 aagccattac aggtcttcgt aaggttgaat ctggtagcat tgagctaaaa ggagattcaa
    21181 ttgtaggctt gcacccacgt cagattacag aactaagtgt tgggcacgtt ccagaagacc
    21241 gtcaccgtga tggcttgatt ttggaaatga tgatatctga aaatattgcc cttcaaacct
    21301 actataaaga accacatagt aaaaatggaa ttttgaacta ttcaaatatt acttcttatg
    21361 ctaaaaagct gatggaagag tttgatgttc gcgctgccag tgaattagtt cctgcagctg
    21421 cactctcagg aggaaatcaa caaaaagcaa ttattgctcg tgaaattgat cgagatcctg
    21481 atctccttat cgttagccag ccaactcgtg ggttggatgt cggtgccatt gagtatatcc
    21541 acaaacgctt gattgaagag cgtgataatg gcaaggctgt ccttgttgtc agctttgaat
    21601 tggatgagat tttaaacgtc tcagaccgta ttgccgttat ccacgatggt aagattcaag
    21661 gtattgtatc accagaaaca accaataaac aagaacttgg tgtcttgatg gctggtggaa
    21721 acttgggaaa ggagaagagt gatgtctaaa aaattacaac aaatttcggt tcccttgatt
    21781 tctgtatttc taggaatttt actcggagcc attgtcatgt ggatcttcgg ttatgatgct
    21841 atttggggct acgaagaatt attctataca gcctttggca gtctgcgtgg gattggagaa
    21901 atcttccgtg ctatgggtcc tctggtcttg attggtcttg gttttgcggt tgccagtcga
    21961 gctggtttct ttaacgtcgg acttcctggt caggctttgg caggttggat tctcagtggt
    22021 tggtttgcgc tgtcgcatcc agatatgccc cgtcccttga tgattctagc aaccatcgtg
    22081 attgccttga ttgctggtgg gattgtcgga gcgattccag gtattcttag ggcctatcta
    22141 gggacgtcag aggttattgt aaccatcatg atgaactaca ttgtcttgta tgtagggaat
    22201 gcctttatcc atgctttccc taaagacttc atgcaaagta cagattcgac cattcgtgtt
    22261 ggggctaatg caacctatca gacaccttgg ttggctgagt tgactggtaa ctcacggatg
    22321 aatattggta ttttctttgc catcattgcc gttgcagtta tttggttcat gctcaagaaa
    22381 acaactcttg gttttgaaat ccgtgcagtt ggtcttaatc cacatgcttc agaatatgct
    22441 ggtatttctg ccaagcggac tattatccta tctatgatta tttcaggtgc cttggcaggt
    22501 cttggtggag ctgttgaagg tttgggaacc ttccagaacg tctatgttca aggttcgtca
    22561 ttagctatcg gatttaacgg aatggcggtt agtttgcttg cggccaactc accaattggt
    22621 atactctttg cagccttcct atttggcgtt ctccaagttg gggctcctgg tatgaatgcg
    22681 gcgcaggtac catctgagct tgtcagcatt gtaacagcgt ctattatctt ctttgtcagt
    22741 gttcattacc ttatcgaacg ctttgtcaaa ccgaaaaaac aagttaaagg aggtaagtaa
    22801 agatgtctat tataaccttg ctccccctct tggtgtcttc tatgctgatt tactcagcac
    22861 ctctcatctt tacaagtatc ggtggtgttt tctctgaacg tggtggtgtg gtaaacgttg
    22921 gccttgaagg aattatggtt atgggtgcct tttctggagt tgtctttaac cttgaatttg
    22981 cagaacaatt tggagcagca actccatggc tatccttgct tgtagcagga ttggttggta
    23041 gtgttttctc tatcatccac gcagcagcga cggttcattt ccgtgcagac catgttgtca
    23101 gcggtacggt attgaacttg atggcgcctg ccttggctgt tttcttagtt aaagttcttt
    23161 ataacaaagg acaaaccgac aacctaagtc aaacttttgg acgctttgat ttcccagtct
    23221 tggcaaatat cccagtgatt ggtgatatct ttttcaagtc aactagtcta cttggttatc
    23281 tggcgattgc cttctcattc cttgcttggt ttattctctt taagactcaa tttggtcttc
    23341 gtctccgctc tgtcggtgaa catcctcaag cagcggatac cttgggaatc aacgtctaca
    23401 agatgagata tttagggatt attatttcag gttttctagg tggaattggc ggagcgattt
    23461 atgcgcaatc aatctcagtt aacttctcag tgacaactat tgttggacct ggatttatcg
    23521 cccttgctgc gatgatcttt gggaaatgga atccaatcgg tgctatgctt tctagtctct
    23581 tctttggact ttcacaaagt ttggctgtta tcggttctca attgccgttc ctacaaggag
    23641 tgccagcggt ttatcttcaa attgcacctt atgttttgac aattcttgtc ttagcagcct
    23701 tctttggaaa agcagtcgca ccaaaagcag atggtatcaa ctacatcaaa tcaaaataag
    23761 catacaaaaa aacgtcagtc caatgactga actgcccccc aaaagttaga cagaaaaaat
    23821 ctaacttttg gggtgttttt attatgaaat taagttatga tgataaagtt cagatctatg
    23881 aactaagaaa gcaaggatat agcttagaga agctttcaaa taaatttggg ataaataatt
    23941 ctaatcttag gta
//
LOCUS       DAVLYV010000024        22547 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLYV010000024 DAVLYV010000000
VERSION     DAVLYV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22547
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.024"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            84..851
                     /locus_tag="VYO70_000949"
     CDS             84..851
                     /locus_tag="VYO70_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEV1713203.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLNKLVSGSNTLTQKSSRLTAGVG"
     gene            936..1616
                     /locus_tag="VYO70_000950"
     CDS             936..1616
                     /locus_tag="VYO70_000950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HEV1713204.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVLVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFSLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
     gene            complement(1717..4269)
                     /locus_tag="VYO70_000951"
     CDS             complement(1717..4269)
                     /locus_tag="VYO70_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1713205.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMGFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIRFHVKLIYIIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(4331..5953)
                     /locus_tag="VYO70_000952"
     CDS             complement(4331..5953)
                     /locus_tag="VYO70_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1713206.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            6159..7184
                     /gene="trpS"
                     /locus_tag="VYO70_000953"
     CDS             6159..7184
                     /gene="trpS"
                     /locus_tag="VYO70_000953"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1713207.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFTKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            7336..8814
                     /gene="guaB"
                     /locus_tag="VYO70_000954"
     CDS             7336..8814
                     /gene="guaB"
                     /locus_tag="VYO70_000954"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1713208.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(8865..9962)
                     /gene="recF"
                     /locus_tag="VYO70_000955"
     CDS             complement(8865..9962)
                     /gene="recF"
                     /locus_tag="VYO70_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1713209.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNRN"
     gene            complement(9965..10333)
                     /locus_tag="VYO70_000956"
     CDS             complement(9965..10333)
                     /locus_tag="VYO70_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1713210.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SCRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            10492..11742
                     /locus_tag="VYO70_000957"
     CDS             10492..11742
                     /locus_tag="VYO70_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1713211.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSEFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGISSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            11739..13022
                     /locus_tag="VYO70_000958"
     CDS             11739..13022
                     /locus_tag="VYO70_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1713212.1"
                     /translation="MTKVVFEEKYYPAVKEKVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            13064..13885
                     /locus_tag="VYO70_000959"
     CDS             13064..13885
                     /locus_tag="VYO70_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1713213.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            13896..14441
                     /gene="pgsA"
                     /locus_tag="VYO70_000960"
     CDS             13896..14441
                     /gene="pgsA"
                     /locus_tag="VYO70_000960"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1713214.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            14438..15265
                     /locus_tag="VYO70_000961"
     CDS             14438..15265
                     /locus_tag="VYO70_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1713215.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPGELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            15250..16089
                     /locus_tag="VYO70_000962"
     CDS             15250..16089
                     /locus_tag="VYO70_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1713216.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            16082..16876
                     /locus_tag="VYO70_000963"
     CDS             16082..16876
                     /locus_tag="VYO70_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1713217.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            16935..17753
                     /gene="mreC"
                     /locus_tag="VYO70_000964"
     CDS             16935..17753
                     /gene="mreC"
                     /locus_tag="VYO70_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1713218.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            17753..18247
                     /gene="mreD"
                     /locus_tag="VYO70_000965"
     CDS             17753..18247
                     /gene="mreD"
                     /locus_tag="VYO70_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1713219.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            18341..19519
                     /locus_tag="VYO70_000966"
     CDS             18341..19519
                     /locus_tag="VYO70_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1713220.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            19743..20522
                     /gene="rpsB"
                     /locus_tag="VYO70_000967"
     CDS             19743..20522
                     /gene="rpsB"
                     /locus_tag="VYO70_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1713221.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            20601..21641
                     /locus_tag="VYO70_000968"
     CDS             20601..21641
                     /locus_tag="VYO70_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1713222.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEDGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            21888..22211
                     /gene="tnpB"
                     /locus_tag="VYO70_000969"
                     /pseudo
     CDS             21888..22211
                     /gene="tnpB"
                     /locus_tag="VYO70_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(22352..>22547)
                     /locus_tag="VYO70_000970"
     CDS             complement(22352..>22547)
                     /locus_tag="VYO70_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEV1713223.1"
                     /translation="PYTPEMNPIEQVWKEIRKRGFKNKAFRTLEDVMNQLQDVIQGLE
                     KEVIKSIVNRRWTRMLFENR"
BASE COUNT         7188 a         3622 c         4616 g         7121 t
ORIGIN      
        1 ttttagacaa tatgtttaaa aaaggagaaa ctaagtttaa agaatggaaa gcaatttaaa
       61 aaaaaccaac ctttattatt gtcatgatcg ggatttctct tattccagat ctgtacaata
      121 tcatattttt gtcatcaatg tgggatccat atgggcaatt gtctgactta cctgtggcag
      181 ttgtaaataa tgataaagag gcttcctata atggtaatac tatggcaata ggaaaagaca
      241 tggtgtccaa tttaaaagaa aataaaacct tggattttca ttttgtagat gaagaggaag
      301 gaaagaaggg attggaagat ggcgattact atatggtagt gactttacca agtgatttat
      361 ctgaaaaaac aactacatta tccaatattc aatcgacagc agcttatcaa tcattgacaa
      421 gtgagcaaca aactgagata agtgattctg tatctcaaaa ttcaactgat agtattcaat
      481 cggctcagtc aattgtagct ttagtacaag atttacaggg aagtttagaa aacttacaaa
      541 atcaatcttc taatctttcg actttaaaaa atcaatctaa tcaagtatca cctattactt
      601 ctacttcttt gataggattg tcaagtggat taacagagat acaaggagat gttactagca
      661 aattagttcc tgccagtcag tcgattgcat caggtgtaaa cgcatatact acaggtgttg
      721 ataaagtttc tcagggcgca agtcaactaa gtgaaaaaaa tgccaccttg acaggtagtt
      781 tgaataaact agtttcaggc tcaaacacct tgacacaaaa atcttctaga ttgacagcag
      841 gagttggtta attacaatca ggatctgggt aattagcaga caaatccagt cagttacttt
      901 caggtgcttc tccattagag aatagagcta ataaattggc agatggatct gggaaactag
      961 cagaaggtgg aacaaagtta acttctggat tggaagattt acagacagga cttgcttctt
     1021 taggacaagg actaggtaat gctagtgatc aactcaaatc agtatcaaca gaatctaaaa
     1081 atgcagagat tttgtcaaat ccactcaatc tttcaaaaac agacaatgat caagttcttg
     1141 taaatggaat cgcaatagct ccttatatga tatcagttgc tctttttgtt gcagcaatat
     1201 caacaaatat gatatttgcg aaattgcctt caggacgtca tccagagagc cgttgggctt
     1261 ggttgaaatc tcgagctgaa ataaatggta ttatagctgt tttggcagga attttggtat
     1321 atggaggagt tcagcttatt ggtttaactg ctaatcatga gatgagaata tttattctca
     1381 tcatcctaac aagtttagta ttcatgtcta tggtgaccac tttaacaacg tggaatagcc
     1441 gtataggagc ttttttctca cttattttgc ttttactaca gttagcatca agtgcaggta
     1501 cttatccact tgctttgaca aatgatttct ttagatctat taatccctgg ttaccaatga
     1561 gctattcagt ttcgggatta cgacaaacaa tctccatcaa caagtcattt tcctagctgt
     1621 catactagct ctatttactg gtttaggtat gctagcctat caacataaga aaatagaaga
     1681 agattaaaaa aatcgaccga ttaactggtc gattttttat gccttagatg actttcgtct
     1741 gtgattatag attccaaata gtaagagaga agtaaaggaa cagattgctc cagtaataaa
     1801 accattggga atgaaggaaa gtgtaatagt tcctttcccc ttgggaatgt caactttcat
     1861 aaatccagtt tgagcttgtt taatttctat tttcttacca tcttggtagg cagaccaacc
     1921 tttgtcataa ggaatggtga agaaaataga tgtatcttgt tggacatcat atgtagcaaa
     1981 aaccttgttt ttagaagttg atactgtgac aggttgttct ttaatttttt gaattgcctc
     2041 ggtgaaagtt ttggtatcta aacgatagaa ggtaggagat tcaaatgata cttgtgaatt
     2101 tccagggaaa ctaacattga tattgaaagt ttttttctct ttagtatatc ctagattaaa
     2161 gaaggagaag acattatcag ttgtaaaagt ctttttttca ccatttacaa ggatgtcaac
     2221 cttcttttgt ttatcgttag aaaagtgaag gtttatgaaa gagagataaa cttggctgtt
     2281 ttctggaact tcaatttgat actggattgc tgcatcttca tttgaagaac ttgtgacact
     2341 aatcaaatca ttagtatttt ctattttttc tgttttttca taaggtattg gagaaaaata
     2401 atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca aggtatgttc
     2461 attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa gagagtattg
     2521 attttcatat agggtaagat tatctttttg atagatatct ttaaagccat acttatcaat
     2581 aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa tactattatt
     2641 tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat ctaaagtaga
     2701 gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt tgaatttcat
     2761 actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat ttccaatata
     2821 tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag aagcaaatcc
     2881 ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt caaccagtat
     2941 aaataaagag attagaatgg caaatagatt cacagatata aactttttga taactgcaag
     3001 gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca aatctgttaa
     3061 aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgttacta caagaaaaag
     3121 cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt ctgctgctgt
     3181 gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt ttggagtatg
     3241 catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta gaaatgcaaa
     3301 gaatattata tatatgagtt tcacgtgaaa cctaatagat ttcagcgtaa aaaataaaat
     3361 ggtcaaaata aagggaagta gtccaacaaa aatcattggg atggccccat actttgttgt
     3421 gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac tttcagtttg
     3481 aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaaca gagtgggaag
     3541 agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat caagaacaga
     3601 tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa taagaaacaa
     3661 tactgtcata tatccaaaat aataattttg aataaataag attgacagac ttgtaaagta
     3721 caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta aaggaatcaa
     3781 gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac ccatcagagc
     3841 ataggaagta gataaggcta gttttaaagt ctgagggata gattgaaaca atttattcaa
     3901 actaaaaaag gttgacagac caatcaatcc aaattttaag agagtggtca gatagacagc
     3961 atctggcata ttcgttagat caaaaaagta aactagaggc gcgagaaaac tacccaagta
     4021 ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt aaaacagact
     4081 actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt gatgaaagcc
     4141 atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata gatatactcc
     4201 agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg tttttaaaaa
     4261 tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca actgagtttt
     4321 gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct gcattttcta
     4381 ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat aagacaatga
     4441 tatggttagc caaagtttga ataaattcgt ggtcatgact agcaaagatg attgattctt
     4501 taaagttttt caatccatca ttcaagcttg agatagattc caagtccaag tgatttgttg
     4561 gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc atgacacgaa
     4621 ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca gagaagagca
     4681 tacggccgag gaaaccacgt aggaaagtat tgtcatcttc ttctttactt gcgaattgac
     4741 gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct tttggtaggt
     4801 aagattgact agttgtaact ccccacttga cagttccttc atagtcaata tctcccatga
     4861 ttgcacgaat taatgcagtc gtttgaatat cattttgtcc aataagtgct gtcttatcat
     4921 ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc tttacagtta
     4981 gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag ttgataaatg
     5041 gatatttacg actagatggt acaatctctt ctagctcaat cttatcaagc attttcttac
     5101 gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaacg aattcttgca
     5161 attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta gcagcaagct
     5221 cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt ccaaagtcaa
     5281 ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat actacgataa
     5341 ctgtgttatc aaagtcaatt aagaagtctt ctaaccaagt aatcgattgg atatccaaac
     5401 cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct ttggcgagga
     5461 gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt aattcttctg
     5521 gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa cctccaagtt
     5581 cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag aaatcttcct
     5641 tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt cccatgataa
     5701 cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga acagagagac
     5761 gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca gctaaaattt
     5821 ttaaaaaggt tgattttccg gcaccattag caccgattaa tccgtaagta tttccttctg
     5881 taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt gaaacatcag
     5941 atactgtaag caatattttt ctcctatatg tgtaatatat ttattctact agaaaataca
     6001 gaaatattca aatttttatt tatcaatttt gtgtaaatta tatttacagt atcctttaca
     6061 caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt cattaaaaaa
     6121 atgctataat tgaaaggact atatcgaagg agaacaaaat gactaaaccc attattttaa
     6181 caggagaccg tccaacagga aaattgcata ttggacatta tgttggaagt ctcaaaaatc
     6241 gagtattatt acaggaagag gataagtatg atatgtttgt gttcttggct gaccaacaag
     6301 ccttgacaga tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga aatgtggctt
     6361 tggattatct tgcagttgga ttggatccaa ataagtcaac tatttttatt caaagccaga
     6421 ttccagagtt ggctgagttg tctatgtatt atatgaatct agtttcgtta gcacgtttgg
     6481 agcgaaatcc aacagtcaag acagagattt ctcagaaagg atttggagaa agcattccga
     6541 caggattctt ggtctatcca atcgctcaag cagctgatat cacagctttc aaggctaatt
     6601 atgttcctgt tgggacagat cagaaaccaa tgattgagca aactcgtgaa attgttcgtt
     6661 cttttaacaa tgcatataac tgtgatgtct tggtagagcc ggaaggtatt tatccagaaa
     6721 atgagagagc agggcgtttg cctggtttag atggaaatgc taaaatgtct aaatcactaa
     6781 ataatggtat ttatttagct gatgatgcgg atactttgcg taaaaaagta atgagtatgt
     6841 atacagatcc agatcatatc cgcgttgagg atccaggtaa gattgaggga aatatggttt
     6901 tccattatct agatgttttt ggtcgtccag aagatgctca agaaattgct gatatgaaag
     6961 aacgttatca acgaggtggt cttggtgatg tgaagaccaa gcgttatcta cttgaaatat
     7021 tagaacgtga actgggtcct attcgtgagc gccgtattga atttactaag gatatgggag
     7081 aagtttataa tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg ggtcaaaccc
     7141 tatctgaggt aaaaggggca atgggacttc attactttaa ctaagtttat tttacaagaa
     7201 actatctttt ctatctcgaa gaaaagatag ttttttattt acccctgtaa catttgacaa
     7261 atttttatag aatggtatga tagatacaat ataaaaagag tcaagctcaa aaagaaagaa
     7321 aagaggaaac ttcgaatgtc taattgggac actaaatttt tgaaaaaagg ttttaccttt
     7381 gatgatgtat tgcttattcc agctgaaagt catgtgttgc ctaacgatgc agatttaaca
     7441 actaaattgg cagataattt gactttaaat atcccaatta ttaccgctgc catggacaca
     7501 gttacagaga gtcaaatggc cattgctatt gctcgtgcag gcggtctcgg agttatccat
     7561 aaaaacatgt caattgctca acaagcagac gaggttcgta aggtaaaacg ttctgaaaat
     7621 ggagttatta ttgatccgtt cttcttgacg cctgaacata caattgctga agcagatgag
     7681 cttatgggtc gttaccgcat cagtggtgtt ccagttgttg aaacacttga aaatcgtaaa
     7741 ttggttggta ttttgacaaa ccgagatctt cgttttattt cagattataa tcaaccaatt
     7801 tcaaaccata tgactagtga aaatcttgtt actgctcctg tgggtacgga tcttgcaacg
     7861 gctgagagta ttcttcaaga gcatcgtatt gaaaaacttc cgttggtcga tgaagaaggc
     7921 agtctttctg gtttgatcac tatcaaagat attgaaaaag ttattgagtt tccaaatgcg
     7981 gctaaagatg agtttggtcg tcttctagtt gcaggtgcag taggtgttac ttcagataca
     8041 tttgaacgtg cagaggctct ttttgaggca ggagcggatg cgattgttat tgatactgca
     8101 catggtcatt ctgcaggtgt cttgcgtaaa attgccgaga ttcgtgctca tttcccagat
     8161 cggactttga ttgctggaaa tattgctact gctgaaggtg cacgtgccct ttatgaagcg
     8221 ggtgtagacg ttgttaaggt tggtattgga ccaggttcta tctgtactac tcgtgtgatt
     8281 gctggtgttg gtgttccgca agtaacagct atctacgatg ctgcagctgt tgcgcgcgaa
     8341 tatggtaaaa cgattattgc tgacggtggg atcaagtatt ctggagatat tgtaaaagca
     8401 cttgctgcag gtggaaatgc tgttatgctt ggatctatgt ttgctggaac tgatgaagct
     8461 ccaggcgaaa ctgaaatctt ccaaggacgt aaattcaaga cttaccgtgg tatgggatca
     8521 attgctgcta tgaagaaagg ttcaagcgac cgttatttcc aaggttctgt caatgaagca
     8581 aacaagcttg ttccagaagg aattgaaggt cgtgttgctt ataaaggagc ggcagctgat
     8641 attgttttcc aaatgattgg tggtattcgc tctggtatgg gttactgtgg tgcagctaac
     8701 cttaaagaac tacacgataa tgctcaattt attgaaatgt ctggtgctgg tttgaaagaa
     8761 agccatcctc atgatgtgca aattactaat gaggcaccaa attattctat gtaaaaaaca
     8821 atgaaaagaa ctccagtgaa aacaggagtt cttttacaat gttgtcaatt tctatttaca
     8881 gcagctttac catcctgaat agtgaagata cttagatttt ctggcagatt ttgaagatgg
     8941 tctaagcttg ttgttgtgat aaaggtttgg attgattgag aaatcgtttc taataatttt
     9001 aactgtctag tgttgtcaag ttcactcatc acatcgtcaa gcagtaatat cggagattct
     9061 gtggtaatgc tttccattaa ttcgatttct gctaatttta tcgagaggac gagactacga
     9121 tgttgacctt ggcttccgaa actagcatcc atcccattta tataaaaaga aatgtcatct
     9181 cgatgaggac cgacaccagt attcttttta aataaatctc tggatctact tttttctaaa
     9241 gcaattttga aagattcgga taagttttgt ttgtcagtta tattgacaga agattgatag
     9301 gatattgaca actcttcgat ctgattagag agttcaaaat gtttcttacg cccaaatgat
     9361 tctagttttt ttatgaaatc taagcggtga ttcattacac gacatccata atcaactagc
     9421 tgatcatcta acacagaaag gaatgtttca tctatttttt gagctgattt taggtaagtg
     9481 tttctttgct ttaggatgtg gttataattg gttaagtcag ataaatagat tggcttaatt
     9541 tgcccaagtt ccatatcaat gaattttcgt cgaatcgaag gtgctccttt aattagttgt
     9601 aaatcttcag gagcaaataa gacaacattc atgtgtccta cataatctga aaggcgtgcc
     9661 tgttttaagt gattaacttt tgttacacgg cctttttgtg ttagttcgat ttctagggga
     9721 atggatccag tttttttctg aacgagacct gaaagatgaa gttgttcctc atcaaaatga
     9781 atgagatttt tatctgttcg agttcgatga ctacgcgtta aggctaaaaa atagatagcc
     9841 tctaacatgt ttgtttttcc ttgtgcatta cgtcccaaaa agacattcaa tttaggatta
     9901 aagtctattt tcgtttcttt gtaattacga aaagtcttga gagatagatg ttgtagccac
     9961 atgattatct accagggaat cgtggagctt gttggctttt aggtgatgaa gcaggtttcg
    10021 atttgtcttt tttaactccc ttattcatct ttttgacaag tttagcgatc cgttcttttt
    10081 caactttatc agcttggtat tcatcttgtt cttcagaagt aggttgtgtc aacaagatgt
    10141 caatgttcat gtcagggatg tcaactttat cgccaatacg aagttttttt ccacgacaac
    10201 tctctaattc cctattaaag taaacagaat gttcagagag aaatgattta atagctcctc
    10261 cgctatgtgt aattccaagt tctttgagta gtgcttggag ggtaataaat tcttcaaata
    10321 atttgtattc cataattcac ctcaatcttt ggtattatac catattttcc taaaaatagt
    10381 gagagagaca gcgagaaatg taagcgattt ttatttcaaa agtgttacag ggaacaagaa
    10441 aatttcaggt tttcgtgata taatagaagt ctgtatataa ggaggtaaat catggagtta
    10501 gtgcatggaa tttcaacaca ttttatccaa tcaaaaaagt ttaaaacaaa caaaattacc
    10561 gtgcgtttta ccgctccatt atcccttgat acgattgcag gtcacatgtt gagtgcaagt
    10621 atgctagaga ctgctaatca gatgtacccc acttctcaag atttgaggag acacttggcc
    10681 agtctatacg gtacagatat gtcaaccaat tgtttcagaa gagggcaaag ccacattata
    10741 gaattgacat ttacctatgt tcgtgatgag tttttaagta ggaaaaacgt gctaacctct
    10801 cagattttgg aacttgtaaa agaaactctt ttttcacccg tagtagttga taatgggttt
    10861 gatccggcct tatttgaaat tgagaaaaaa caattgctag caagtttagc agctgatatg
    10921 gatgattctt tttattttgc acataaagaa ttggataaat tgttttttca tgatgaacgt
    10981 cttcaattgg aatatagtga tttacgaaat cgtattttag ctgaaactcc acaaagttct
    11041 tattcttgtt tccaagaatt tttagccaat gatcgaatag atttcttttt cctaggtgat
    11101 tttaatgagg ttgaaattca aaatgtatta gaatcatttg gctttaaagg tcgaaaagga
    11161 gatgtgaagg ttcagtattg tcaaccttat tctaatatcc ttcaggaagg tatggttcgg
    11221 aaaaatgtgg gacaatccat tttggaatta ggttatcatt accgttctaa atatggtgat
    11281 gagcaacatt tacccatgat tgtaatgaat ggtttacttg gtggatttgc tcactctaag
    11341 ctctttacaa atgtccgtga aaatgctgga ttagcttata ccatttcaag tgagcttgat
    11401 ttatttagtg aattcttgag gatgtatgct ggtatcaatc gagaaaatcg taaccaggct
    11461 cgtaaaatga tgaataatca actgcttgat ttaaaaaaag gttattttac agagtttgag
    11521 ttaaatcaga ccaaggaaat gattcgttgg tcgttgttac tttctcaaga taatcaatct
    11581 tcattgattg aacgtgctta tcaaaatgcc ttatttggaa tatcttcagc agactttaaa
    11641 ggttggattg caaaactcga gcaagttgac aaagatgcta tttgtagagc agctaataat
    11701 gtgaaactac aagcgattta ctttatggaa ggaatagaat gacaaaggtt gtttttgaag
    11761 aaaaatacta tccagctgta aaagaaaagg tttatcgaac tcgtttggcc aatggattga
    11821 cagttgctct tttgcctaaa aaggaattta aagaggttta cgggagtgtc actgtacagt
    11881 ttggttcggt agatacgttt gtcacagaag ttgacggaga tgtaaaacaa tatcctggag
    11941 gaattgctca ttttcttgaa cataaattat ttgagagaga agattctagt gatttgatgt
    12001 cggcttttac gagtctaggt gcagatagta atgcctttac aagctttaca aaaacaaact
    12061 atcttttttc agcaacggat tattttttag aaaatttaga tttacttgat gaattggtaa
    12121 catcagcaca ctttactgaa gcttccattc tgacagagca ggatattatt cagcaagaac
    12181 gagaaatgta ccaagatgat ccagattcgt gtttattctt ttcaacttta gcgaatttgt
    12241 atcctggtac acctttagca actgatatag ttggaagtga ggagtccatt tcccaaatca
    12301 atctaactaa tttgcaagaa aattttacaa agttttacaa acctgtaaac atgtctctgt
    12361 ttttagttgg taattttgat gtggagcgag tacaggacta ttttgaaagc aaagaactga
    12421 aagattcaga ttttcaggaa gtagcaagag aaaagttgtt tttacagcct gtaaagccaa
    12481 cagatagtat gagaatggaa gtatcttctc ccaaactagc gattggagtt agaggtaagc
    12541 gagaagtttc tgaagcggat tgctatcgac atcatatttt attaaaatta ttgtttgcaa
    12601 tgatgtttgg ttggacttcg gatcgttttc aaaaatgtta tgaatcaggt aaaattgatg
    12661 catccttatc tctggaagtt gaagtaacaa gtcgctttca ttttgtcatg ttgacaatgg
    12721 atacgaaaga gccagttgct ttgtctcatc aatttaggaa ggctattcgt aattttacaa
    12781 aggatttaga tattacagag gaacatttag atattatcaa aagagagatg tttggcgaat
    12841 ttttcagtag catgaactct cttgaattta ttgcaacgca atatgatgct tttgaaaatg
    12901 gtgagacaat ttttgatttg ccgaaaattt tacaggaaat tactttagag gatgtccttg
    12961 atgctggaca tcatttaata gatgatggtg acatagttga ttttacaata ttcccatcgt
    13021 agtaacctat tataatagac actagaaaga agggatgaca agtatgagaa aaaaaacaat
    13081 tggagaggtt ttacgattag ctagaatcaa tcagggattg agtttagatg aattgcagaa
    13141 aaagacagaa atccagttag atatgttgga agcaatggaa gcagacgatt tcgatcaact
    13201 tccaagtcct ttttacacgc gttctttctt gaaaaaatat gcatgggctg ttgagttaga
    13261 tgaccaaatt gttttggatg cttatgattc tgggagtatg attacttatg aggaagtaga
    13321 tgttgatgaa gatgagttga caggtcgtag acgttcaagt aagaaaaaga agaaaaaaac
    13381 atcattttta cctttatttt attttatcct ttttgcttta tcgattttaa tttttgtgac
    13441 ttattatgtt tggaactata ttcaaactca accagaggag ccttctcttt ctaattacag
    13501 tgtggttcaa tcaacaagtt caactagctc tgttccccac tcctcaagta gtagttcttc
    13561 tagtatagaa tcagctataa gtgtatcagg cgaaggaaat catgtagaaa tcgcttataa
    13621 gacaagtaag gaaacagtta aattgcaatt ggcagtttca gatgttacaa gttgggtcag
    13681 tgtttcagaa agcgaacttg agggcggtgt aaccttatcg ccaaagaaga aaagtgcaga
    13741 agcaacagtt gcaactaaaa gtcctgtaac aattacgtta ggtgttgtaa aaggtgttga
    13801 tttgacagta gataatcaga ctgttgattt atcgaaatta acagctcaga ctggacaaat
    13861 cactgtaacc tttactaaaa attaaggaaa aacgaatgaa aaaagaacaa attcccaatc
    13921 tcttaacaat aggtcgaatt ctctttatac ctatttttat ctttatttta acgataggaa
    13981 attcgataga gagtcatata gttgcagcta ttatctttgc tgttgccagt attaccgact
    14041 atttagatgg atatttagct cgtaaatgga atgtggtcag taattttggt aaatttgcag
    14101 atcctatggc ggataagtta ctagttatgt cggcttttat tatgttgatt gagttaggta
    14161 tggctccggc ttggattgtt gcagtgatta tctgtcgtga gttagctgtg acaggtttaa
    14221 ggcttttatt ggttgaaact ggtggaacaa ttttagcagc agcaatgcct ggaaaaatta
    14281 aaacttttag tcagatgttt gctattattt tcttgctatt acattggact ttgcttggtc
    14341 aagttctact ttatgtagcc ttatttttca ctatctactc tggctatgac tatttcaagg
    14401 gtagtgccta tgtatttaaa gggacatttg gttcgaaatg aaatcaataa ttgatgtaaa
    14461 aaatctttct tttcgctata aagaaaatca gaactactac gatgtgaagg atattacgtt
    14521 tcacgtgaaa cgtggagaat ggctttcgat tgtagggcat aatggtagtg gtaaatcaac
    14581 gacggttcga ttaattgatg gcttactgga agcagaatcc ggagagattg taattgatgg
    14641 ccaacgtttg actgaggaaa atgtttggaa tatacgtcgt caaatcggta tggtttttca
    14701 aaatccagac aatcaatttg ttggagcgac tgttgaagat gatgttgctt ttggtttgga
    14761 aaatcaggga ctttctcgtc aagaaatgaa aaagagagtg gaagaagctc tggctttagt
    14821 tggcatgttg gactttaaaa agagagagcc agcgcgtcta tcaggtggcc aaaagcaacg
    14881 tgtggccatt gcaggtgttg tagccctaag accagctatt ttaatcttag atgaagcaac
    14941 gagtatgttg gatcctgagg ggcgtagaga acttattggg acagtaaaag gaattcgaaa
    15001 agactatgat atgacggtca tttctattac ccatgatttg gaagaagtcg ccatgagtga
    15061 tcgcgtattg gtcatgaaaa aaggggaaat tgaatcaact agtagtccag gggagctttt
    15121 ctctcgaaat gatttagatc aaattggatt agacgatcct tttgctaatc aattaaaaaa
    15181 atctttgagc cagaatggct atgatttacc tgaaaattat ttgacagaaa gtgagctaga
    15241 ggataagcta tgggaattgc tctagaaaat gtgaatttta catatcaaga aggtactccc
    15301 ttagcttcag cagctttgtc ggatgtttct ttgacgattg aagatggctc ttatacagct
    15361 ttaattgggc acacaggtag tggtaaatca actattttac aactcttaaa tggtttattg
    15421 gtgccaagtc aagggagtgt gagggttttt gataccttaa tcacctcgac ttctaaaaat
    15481 aaagatattc gtcaaattag aaaacaggtt ggcttggtat ttcagtttgc tgaaaatcag
    15541 atttttgaag aaacggtttt gaaggacgtt gcttttggac cgcaaaattt tggagtttct
    15601 gaagaagatg ctgtgaagac tgcgcgtgag aaactggctc tggttggaat tgatgaatca
    15661 ctttttgatc gtagtccgtt tgagctgtca gggggacaaa tgagacgtgt tgccattgca
    15721 ggcatacttg ccatggagcc agctatatta gtcttagatg agccaacagc tggtctagat
    15781 cctctaggga gaaaagagtt gatgaccctg ttcaaaaaac tccaccagtc agggatgacc
    15841 atcgtcttgg taacgcattt gatggatgat gttgctgaat atgcaaatca agtctatgta
    15901 atggaaaagg gacgtttagt aaaggggggc aaaccaagtg atgtctttca agacgttgtt
    15961 tttatggaag aagttcagtt gggagtacct aaaattacgg ccttttgtaa acgattggct
    16021 gatagaggcg tgtcatttaa acgattaccg attaagatag aggagtttaa ggagtcgcta
    16081 aatggatagt atgattttgg ggcgttatat cccaggggat tcgattgttc accgattgga
    16141 tccacgtagc aaattgcttg ctatgatgct actgattttg attgtgtttt gggctaataa
    16201 ccccttgacg aatctgattc tttttatagc gacagggata tttattgcct tgtcaggagt
    16261 atctctttca ttttttattc agggcttgaa gtctatgttt ttcttgattg ccttcacaac
    16321 tatttttcaa ctatttttca tttctaatgg gaatgtttta tttgagtttt cgtttgtgag
    16381 aatcacggat tatgctttgc aacaagctgg gattattttt tgtcgctttg tattgattat
    16441 tttcttttca actttgttaa ccttaacaac catgccttta agtttggcat cagctgtcga
    16501 agctttatta gcacctttaa agcgtgtgaa agttccagtt catgaaattg gattgatgct
    16561 gtctatgagt ttgcgttttg tcccaacctt gatggatgat acgacgcgga ttatgaatgc
    16621 acagaaagct cgtggagtgg attttggaga aggaagtatc gttcaaaaag taaaggcgat
    16681 gattcccatt ttgattcctc tttttgcgac aagtttaaaa cgtgcagatt ccttggctat
    16741 cgctatggaa gcgcgtggct atcagggtgg aaaaggcaga agtcaatacc gacaattgaa
    16801 atggactcta aaggatacgc tgaccattct tgttattctc gtacttggtt gttgtttatt
    16861 tttcttaaaa tcttagtagc tttcaggaat tgataaaaag ttactgtaac agtttttgat
    16921 ataaaggtag ggatatgaac cgttttaaaa aatcaaaata tgtcattatt gtttttgtca
    16981 ctgttctgct tgtgtcagct ctcttagcga cgacttattc aagtacaatt gtgacaaaat
    17041 taggagatgg aatctcattg gttgatagag ttgtacaaaa accttttcag tggtttgatt
    17101 ctgtcaaatc agatttggct catttgacac gaacatataa tgaaaatgaa agtttgaaga
    17161 aacagcttta ccaattagaa gttaaatcaa atgaggtgga aagtttaaag acagaaaatg
    17221 aacaactgcg ccaattgctt gatatgaagt ctaaattgca agccacaaag actttagcag
    17281 cagatgttat tatgcgttct ccggtatctt ggaagcagga gttgacctta gatgcaggta
    17341 gatcaaaagg tgcttctgag aacatgttag ctattgcaaa tggtggcttg attgggagtg
    17401 tttcaaaagt agaggagaac tctactatag tcaaccttct gacaaatacg gaaaatgctg
    17461 ataagatttc tgttaaaatt caacatggct ctactacaat ttatggaatt attattggct
    17521 atgacaagga aaatgacgtt cttaaaatta gccaattaaa tagtaatagc gatattagtg
    17581 cgggagataa ggtgactact ggtggattag gaaactttaa cgttgctgat attcctgttg
    17641 gtgaagtggt tgccacaacg catagtacag actatttgac acgagaagta actgttaaat
    17701 tgagtgcaga tactcataat gtagatgtga tagaattagt ggggaattca taatgagaca
    17761 gttgaagcga gttggagtat ttttattgct tcctttcttt gttctaattg acgcccatat
    17821 tagccagctt ctgggctcat ttttccccca tgtacatttg gctagtcatt ttctttttct
    17881 atttctctta tttgagacga tagaagtatc agagtatctc tacctagtct attgttttgt
    17941 tataggcttg gtttatgatg tttacttttt ccatctaata gggattacaa ctctcttatt
    18001 tatcttattg ggagccttcc ttcataaatt gaatagtgtt attttgttga atcgttggac
    18061 aagaatgcta gctatgattg tgctgacatt cctgtttgaa atgggtagtt atcttttggc
    18121 ttttatggta gggttgacag tagatagcat gtcgattttt atagtctata gcttggtacc
    18181 gacgatgatt ttaaattttt tatggattac tgtttttcaa tttatttttg aaaaatatta
    18241 tctataagaa cgacatataa atgtaacaaa ggcgtaatat ttattaggcc tttttttggt
    18301 atactagtat tgtctttaaa agaaggagta tctacgtaat atgaagaaaa aaatcttagc
    18361 gtcactttta ttaagtacag taatggtttc tcaagtagct gttttaacaa ctgcgcatgc
    18421 agaaacgact gatgacaaaa ttgctgctca agataataaa attagtaact taacagcaca
    18481 acaacaagaa gcccaaaaac aagttgacca aattcaggag caagtatcag ctattcaagc
    18541 tgagcagtct aacttgcaag ctgaaaatga tagattacaa gcagaatcta agaaactcga
    18601 gggtgagatt acagaacttt ctaaaaacat tgtttctcgt aaccaatcgt tggaaaaaca
    18661 agctcgtagt gctcaaacaa atggagccgt aactagctat atcaatacca ttgtaaactc
    18721 aaaatcaatt acagaagcta tttcacgtgt tgctgcaatg agtgaaatcg tatctgcaaa
    18781 caacaaaatg ttagaacaac aaaaggcaga taaaaaagct atttctgaaa aacaagtagc
    18841 aaataatgat gctatcaata ctgtaattgc taatcaacaa aaattggctg atgatgctca
    18901 agcattgact acgaaacagg cagaactaaa agctgctgaa ttaagtcttg ctgctgagaa
    18961 agcgacagct gaaggggaaa aagcaagtct attagagcaa aaagcagcag ctgaggcaga
    19021 ggctcgtgca gctgcggtag cagaagcagc ttataaagaa aaacgagcta gccaacaaca
    19081 atcagtactt gcttcagcaa acactaactt aacagctcaa gtgcaagcag tatctgaatc
    19141 tgcagcagca cctgtccgtg caaaagttcg tccaacatac agtacaaatg cttcaagtta
    19201 tccaattgga gaatgtacat ggggagtaaa aacattggca ccttgggctg gagactactg
    19261 gggtaatgga gcacagtggg ctacaagtgc agcagcagca ggtttccgta caggttcaac
    19321 acctcaagtt ggagcaattg catgttggaa tgatggtgga tatggtcacg tagcggttgt
    19381 tacagctgtt gaatcaacaa cacgtatcca agtatcagaa tcaaattatg caggtaatcg
    19441 tacaattgga aatcaccgtg gatggttcaa tccaacaaca acttctgaag gttttgttac
    19501 atatatttat gcagattaat ttacagaggg actcgaatag agccctcttt tcaggtttta
    19561 ccgtgacaat ctctattaaa aattatatca aaatagcttg aaaatattgg aaaagtatgg
    19621 tagaatgaaa attgtcgtgt gaacgataat actcattctt gatgaattgt gaagcagttg
    19681 cccttgggtc gttttgcgag ttgaagtcaa gaagaggaaa aaaacaaaaa ggagaaatac
    19741 tcatggcagt aatttcaatg aaacaacttc ttgaggctgg tgtacacttt ggtcaccaaa
    19801 ctcgtcgctg gaatcctaag atggctaagt acatctttac tgaacgtaac ggaatccacg
    19861 ttatcgactt gcaacaaact gtaaaatacg ctgaccaagc atacgacttc atgcgtgatg
    19921 cagcagctaa cgatgcagtt gtattgttcg ttggtactaa gaaacaagca gctgatgcag
    19981 ttgctgaaga agcagtacgt tcaggtcaat acttcatcaa ccaccgttgg ttgggtggaa
    20041 ctcttacaaa ctggggaaca atccaaaaac gtatcgctcg tttgaaagaa attaaacgta
    20101 tggaagaaga tggaactttc gaagttcttc ctaagaaaga agttgcactt cttaataaac
    20161 aacgtgcgcg tcttgaaaaa ttcttgggcg gtatcgaaga tatgcctcgt atcccagatg
    20221 tgatgtacgt agttgaccca cataaagagc aaatcgctgt taaagaagct aaaaaattgg
    20281 gaatcccagt tgtagcgatg gttgacacca atactgatcc agatgatatc gatgtaatca
    20341 tcccagctaa cgatgacgct atccgtgctg ttaaattgat cacagctaaa ttggctgacg
    20401 ctattatcga aggacgtcaa ggtgaggatg cagtagcagt tgaggcagaa tttgcagctt
    20461 cagaaactca agcagattca attgaagaaa tcgttgaagt tgtagaaggt gacaacgctt
    20521 aatttataca aatagtaatt acctaggagg gcggggctta gcccggctct cctattttca
    20581 aaaaatatag gagaattaaa atggcagaaa ttacagctaa acttgtaaaa gagttgcgtg
    20641 aaaaatctgg tgccggtgtt atggacgcta aaaaagcgct tgtagaaaca gacggtgaca
    20701 tcgaaaaagc gattgaattg cttcgtgaaa aaggtatggc taaggcagct aagaaagctg
    20761 accgtgttgc tgcagaaggt ttgactggtg tttatgttaa cggtaatgtt gcagcagtta
    20821 ttgaagtaaa cgctgaaact gacttcgttg caaaaaacgc tcaattcgtt gaattggtaa
    20881 atactacagc taaagtcatt gctgaaggaa aacctgctaa caacgaagaa gctcttgctt
    20941 tgataatgcc ttcaggtgaa actcttgaag ctgcatacgt atctgcaaca gcaactatag
    21001 gagagaaaat ctcattccgt cgctttgcat tgattgaaaa aacagacgca caacactttg
    21061 gagcatacca acataacggt ggacgtatcg gtgttatttc agttgttgaa gatggagacg
    21121 aagcacttgc taaacaattg tcaatgcaca tcgcagcgat gaaaccaaca gttctttctt
    21181 acaaagaatt ggatgagcaa ttcgttaaag atgagttggc acaattgaat cacgttatcg
    21241 accaagacaa cgaaagccgt gcaatggtta ataaaccagc tcttccacac ttgaagtatg
    21301 gatcaaaagc tcaattaact gatgatgtta ttgctcaagc tgaagctgac atcaaagctg
    21361 aattggctgc agaaggcaaa ccagaaaaaa tctgggacaa aattattcca ggtaaaatgg
    21421 atcgcttcat gcttgataac actaaagttg accaagctta cacacttctt gcacaagttt
    21481 acatcatgga tgacagcaag acagttgaag cataccttga atcagttaac gcttcggtag
    21541 ttgagtttgc tcgctttgaa gttggtgaag gtatcgagaa agctgcaaac gacttcgaag
    21601 ctgaagttgc agctacaatg gcagcagcct tgaataacta ataatagaaa ggaagcaaat
    21661 tgcttccttt tttgtatagg gcttagattt ttcctgaaat tgttttttcc ccagtctcac
    21721 tcgattagaa taattaaaac aaaaagccct ataataaggg ctaagtgcat ttaggagact
    21781 atacttaaaa ttcatagtaa gcgcgttcat aacaaggtat ctatcctaat tccccaactt
    21841 ccagtatact agataaaaaa taattatcgg agtaaaggta ttgtactatg ataattcaac
    21901 taagtgattt aggtcaagtt caccttgttt gtggcaagac agatatgagg tagggaatag
    21961 actcattagc ctatgtagtt aaaacccact ttgaattgga ttctttctcc ggtcaagtct
    22021 ttctcttttg tggtgaacgt aaagaccgct ttaaagccct ttactgggat ggtcaaggat
    22081 tttggctact atataaacgc tttgagaacg gaaaactgac ttggctaagt acagaaaagg
    22141 atgtcaaagc tctcgcacct gaacaagtag attggcttat gaagggcttt tctatcactc
    22201 cccaaatata gtggatttaa actagaatag tacacctctg cttctaaaac attgttagaa
    22261 atcgatttga ctatcctgtt cttatttcat tttactataa attcatcaga aagtcgtgat
    22321 ttctattgaa atgaggactt tctttttata ctcatctgtt ttcaaaaagc attctagtcc
    22381 atctccgatt aacgatggac tttatcacct ccttctccag tccttgtatg acatcttgga
    22441 gttgattcat gacatcttcc aaagttcgaa aggccttatt cttaaatcca cgtttacgaa
    22501 tctctttcca cacttgttca atggggttca tctctggtgt gtatgga
//
LOCUS       DAVLYV010000025        22322 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLYV010000025 DAVLYV010000000
VERSION     DAVLYV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22322
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.025"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VYO70_000971"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VYO70_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1713224.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPYRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VYO70_000972"
     CDS             2483..4144
                     /locus_tag="VYO70_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1713225.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFNIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILEIGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMSYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VYO70_000973"
     CDS             4213..4992
                     /locus_tag="VYO70_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1713226.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VYO70_000974"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VYO70_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1713227.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMSTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VYO70_000975"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VYO70_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1713228.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7640..8341
                     /locus_tag="VYO70_000976"
     CDS             7640..8341
                     /locus_tag="VYO70_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1713229.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8381..10225
                     /gene="uvrC"
                     /locus_tag="VYO70_000977"
     CDS             8381..10225
                     /gene="uvrC"
                     /locus_tag="VYO70_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1713230.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPSI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            10215..11048
                     /locus_tag="VYO70_000978"
     CDS             10215..11048
                     /locus_tag="VYO70_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1713231.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDCVLEVLDGQYGLENPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11230..12030)
                     /locus_tag="VYO70_000979"
     CDS             complement(11230..12030)
                     /locus_tag="VYO70_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1713232.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12201..12806
                     /locus_tag="VYO70_000980"
     CDS             12201..12806
                     /locus_tag="VYO70_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1713233.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            12819..14219
                     /gene="pepV"
                     /locus_tag="VYO70_000981"
     CDS             12819..14219
                     /gene="pepV"
                     /locus_tag="VYO70_000981"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1713234.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14273..14851
                     /locus_tag="VYO70_000982"
     CDS             14273..14851
                     /locus_tag="VYO70_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1713235.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15011..15163
                     /locus_tag="VYO70_000983"
     CDS             15011..15163
                     /locus_tag="VYO70_000983"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1713236.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            15206..15298
                     /locus_tag="VYO70_000984"
     CDS             15206..15298
                     /locus_tag="VYO70_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1713237.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15469..16794
                     /gene="brnQ"
                     /locus_tag="VYO70_000985"
     CDS             15469..16794
                     /gene="brnQ"
                     /locus_tag="VYO70_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1713238.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            16892..17929
                     /locus_tag="VYO70_000986"
     CDS             16892..17929
                     /locus_tag="VYO70_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1713239.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDGQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KEQNIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYIKSALVD"
     gene            17931..18401
                     /locus_tag="VYO70_000987"
     CDS             17931..18401
                     /locus_tag="VYO70_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1713240.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLEKETRLRFLEEMSLIQEAVAKAFTAEKMNIELLGNGDAHLHWHLFPRR
                     TGDMNGHGLKGRGPVWWVPFEEMTAETCQAKPDEIKRLVKRLSSEVDKLLEIKE"
     gene            18404..19120
                     /locus_tag="VYO70_000988"
     CDS             18404..19120
                     /locus_tag="VYO70_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1713241.1"
                     /translation="MKKRYLVVTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(19160..19297)
                     /locus_tag="VYO70_000989"
     CDS             complement(19160..19297)
                     /locus_tag="VYO70_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713242.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            19429..19854
                     /gene="rplK"
                     /locus_tag="VYO70_000990"
     CDS             19429..19854
                     /gene="rplK"
                     /locus_tag="VYO70_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1713243.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            20062..20751
                     /gene="rplA"
                     /locus_tag="VYO70_000991"
     CDS             20062..20751
                     /gene="rplA"
                     /locus_tag="VYO70_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1713244.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            21129..21266
                     /locus_tag="VYO70_000992"
                     /pseudo
     CDS             21129..21266
                     /locus_tag="VYO70_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(21649..21729)
                     /locus_tag="VYO70_000993"
                     /pseudo
     CDS             complement(21649..21729)
                     /locus_tag="VYO70_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            21737..22099
                     /locus_tag="VYO70_000994"
     CDS             21737..22099
                     /locus_tag="VYO70_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1713245.1"
                     /translation="MLEVGLNFLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         6673 a         4138 c         5140 g         6371 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 ggctcggtta ttgtttgaga gagggattca ggaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctc gtttatggag actatgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgcggagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag gaatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatgcct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ctaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gccgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccctatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaagg cttattatct
     1981 gacaggttat gggactagag atcagtttgc caaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtatcagaa cttttagaat aagagggtag aattgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctaccgt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttaatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctt ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagata ggtcgtatgg gtgagcctat caatggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctagtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacgca tcttcattcc
     3721 taaaaagggg acgaccatgt cttacgagaa tggagacttc gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag gtcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcacacgcgt ggatttgttt atctcaagaa
     3961 gagtcgcgat attctccgtg aaagttcaga attgattaac caaacggtag aagagtatct
     4021 tcaaggagat gactttgact gggcagatct caaaggtaag gttcgtgaca atctgaccaa
     4081 gtacctcttt gatcaaacca agcgtcgccc agccatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacga gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggctacggc tgcttcaaac tggctcttac gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagtgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg aaaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga gttagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acctttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgtcca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcttgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccttatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatc caatagatgc tgagaaaaaa agtattattc aaggtttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttga atttttcgga gtatatgagc aaattgcaag gtgaacaaag
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 aaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctgggag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg ccctgcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcgggta ctttccgtta
     7501 ccatccatcg cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagtaaaata attcttattt
     7621 gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt tcttttcttt
     7681 tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg tgcttgcttt
     7741 cggacttctc tggtatctga acggggattt tcaaggagcg ctaaagcaag cagaacggtc
     7801 agtaaaaatt ggtcaacaaa gtattgacca atgggagaaa acagggcaac tgcctaagtt
     7861 aagccagaca gatagtcacc agcattctga aggaaggtgg gcacaggcct ctgctcgtat
     7921 ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag caatccagaa
     7981 ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta aggcggatat
     8041 tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg aaagtcaaac
     8101 taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt attatttgtc
     8161 caatccatac tatggctact cctatgaacg ccttgtccat acggcagaac atgagttagg
     8221 tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag caggttcctt
     8281 ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga ctagtgagta
     8341 gggtactatc tttccctact ttttttgcta taatggaact atgaacaact tgattaaatc
     8401 aaaactagag ctcttgccga ccagccctgg ttgctacatt cataaggata aaaatggcac
     8461 cattatctat gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct attttcgtgg
     8521 aagtcatgat accaagacag aggctctggt gtctgaaatt gtggattttg aatttattgt
     8581 tacggagtct aatattgagg cacttctcct agaaatcaac ctgatcaagg aaaacaagcc
     8641 caagtacaat atcatgctca aggatgacaa gtcctatcct tccatcaaaa tcaccaatga
     8701 gcgctatcca cgcttgatta tcactcgtca ggtcaaaaag gacggaggtc tttattttgg
     8761 accctatccc gatgtggggg cagccaatga aatcaagcgg ttgctggatc ggatattccc
     8821 ttttcgtaag tgtaccaacc cgccctctaa ggtctgtttt tattaccata tcggtcaatg
     8881 catggcccac accatctgta agaaggatga gacttatttc aagtctatgg cccaggaggt
     8941 ttctgatttt ctgaaagggc aggataacaa aatcattgat gagctaaagg gcaaaatggc
     9001 agcggcagcc cagactatgg agtttgaacg tgcggcggaa taccgtgacc tgattcaggc
     9061 tattggaacg cttcgaacca agcaacgggt catggctaag gatctccaaa atcgcgatgt
     9121 ctttggctac tatgtggata agggctggat gtgtgtgcag gttttctttg tccgtcaggg
     9181 taagctcatc gagcgcgatg tcaatctctt cccctacttc aatgatccag atgaggattt
     9241 tttgacctat gtaggacaat tctatcaaga aaaatctcac ctagttccca atgaggtact
     9301 gattccgcag gatattgacg aagaagctgt caaggctttg gtggatacca agattctcaa
     9361 accccaacgt ggagagaaaa aacagctggt caatctagcc ataaaaaatg cacgtgttag
     9421 tctggagcag aaatttaacc tgctagagaa atctgtcgag aagacccaag gagctattga
     9481 aaatctagga cgtttgctcc aaatcccgac cccagtacgt atcgagtcct tcgataactc
     9541 taatatcatg ggaactagcc ctgtttcggc tatggtggtc tttgtcaacg gtaaaccgag
     9601 taagaaggat taccgcaagt acaagataaa aacggttgtt ggaccagacg actatgccag
     9661 catgagagag gtcattcgca gacgctatgg tcgagtacag cgtgaggctt tgactcctcc
     9721 agatttgatt gtgattgatg gggggcaagg tcaagtcaat atcgctaagc aggttatcca
     9781 agaggaactg ggcttggata ttccaattgc tgggctgcaa aagaatgata agcaccaaac
     9841 ccatgaactg ctctttggag atccgcttga ggtggtggac ttgtctcgca attctcagga
     9901 atttttcctc ctccaacgca tccaagatga ggtgcaccgc tttgccatta ccttccaccg
     9961 ccaactgcgc tccaaaaatt ctttctcatc tcaattggat gggattgatg gtctgggacc
    10021 taaacgcaag caaaatctca tgaggcactt caagtctctg accaaaatca aggaagccag
    10081 tgtggatgag attgtcgaag ttggagtacc aagagcagta gcagaggctg tgcaaagaaa
    10141 gttgaacccg cagggagaag ccttgcctca agtagcagaa gaaagagtag attaccaaac
    10201 ggaaggaaac cacaatgaac cataaaatcg caattttatc agatgttcat ggcaatgcga
    10261 cggcgctaga agcagtgatt gcagatgcta aaaatcaagg ggccagtgaa tattggcttc
    10321 tgggagatat ttttcttcct ggtccaggcg caaatgactt agtcgccctg ctaaaggacc
    10381 ttcctatcac agccagtgtt cgaggcaatt gggatgattg tgtcctggag gtcttggatg
    10441 ggcaatacgg tttggaaaat ccgcaagaaa tccagctcat gcgaatgact cagtttttga
    10501 tggagcgaat ggatcctgca acgactgtct ggctacgaag cttgcctttg ctggaaaaga
    10561 aagaaattgc tggactgcgc ttttctatct ctcataattt gccaaataag aactatggtg
    10621 gtgacttgct agttgagaat gatacagaga aatttgacca actgctagat gcggaaacgg
    10681 acgtggcagt ttatggtcat gttcacaagc agttgcttcg ttatggaagt caagggcaac
    10741 aaatcatcaa tccagggtcg attggcatgc cctattttaa ttgggaggcg ttaaaaaatc
    10801 accgtgccca gtatgccgtg atagaagttg aagatgggga attggtcaat atccaatttc
    10861 gtaaagttgc ttatgattac gaggctgagt tagaattggc caagtccaag gggcttccct
    10921 ttattgaaat gtatgaagaa ctgcgtcgtg acgataacta tcaggggcac aatctggaac
    10981 ttttagccag cttaatagaa aagcatgggt atgtagagga tgtgaagaat ttttttgatt
    11041 ttttgtaaga gtttcctaaa atagccaatg caaactaaaa aagcgattgg ctggtccaat
    11101 cgcttttagt atatcttata ctcaatgaaa atcaaagagc aaactaggaa gctagccgta
    11161 ggttgctcaa agcacagctt tgaggttgca gataaagctg acgtggtttg aagagatttt
    11221 cgaagagtgt tattgtaact gagattgatc tgggaggtaa gaaccaccta gataggtatt
    11281 gctgagtttt tcaagggttc cgtcttgata gagttctttg agcgctttat caaattgctc
    11341 tttaaactct ttttggtcgc ttgagaaaat gatataattg ctggggctat ctgcagaagg
    11401 taaatcaacg actgagaggt ctaaaccacg gtccttgata atcttttgaa cggatacctt
    11461 gtcaaaaact aggaaatcaa actctccgtt agcaaggtct aggattcgtt taccaatatc
    11521 ctcaccagaa aaattaattg tagcgggatt atcagtgtgt ttctgattcc agttattgat
    11581 gaattgagcg ttagaagttc cggtatcctc ttgtgttgtt ttaccagcga tctggtcaag
    11641 agaagtcaaa ggatttttct tgttgctgac aaggacgagg ggattgttgg aaatcggaag
    11701 cgagtaaagg tatttttcag cacgctcttt tgtgtaactc aaattattgg ccgcagcctg
    11761 atagtgacca gaatcaagtc ctgggaagat actttcccag gcggttcttt gaaattgaat
    11821 ctcgtagtcg ctgagttttt catctactgc ctttagaact tcgatatcga agcctgtcag
    11881 attgccttta tcttcgtagt caaatggtgg cacgtcgcca gctgtagcaa ggacgattgt
    11941 cttttgagcg ctagtctctt tgggtgtagc ttgattccca caggcaacca aaaatggtag
    12001 gatagctagt aataggctaa attttttcat actgtctcca ttcaaatgta aagtacttgc
    12061 tagtttatca gaaactttcc tgataaacca atatatatct tttatgtctg tgatagaaaa
    12121 acttaatcat gaaaaatccc tgcaagctct ttcaaattat ggtagaatgg aagcagtaga
    12181 attagaaaag gaaatcgatt atgaaatttc ttgaattaaa taaaaaacgt catgcgacca
    12241 agcatttcac tgataagcct gttgatccaa aagatgtgcg tacggctatc gaaattgcaa
    12301 ccttggcgcc aagcgcccac aacagccagc cttggaaatt tgtagttgtt cgtgagaaaa
    12361 atgctgaact ggcaaagttg gcttatggtt caaactttga gcaggtatca tcagcacctg
    12421 taaccattgc cctatttaca gatacagacc ttgccaaacg cgctcgtaag attgcccgag
    12481 ttggtggtgc taacaatttc tcagaagaac aacttcaata cttcatgaaa aatttgccag
    12541 ctgaatttgc acgctacaat gaacaacaag tcagcgacta cctagcactc aatgcaggtt
    12601 tggttgctat gaacttggtt ctcgctctta cagaccaagg aattggttcc aatattatcc
    12661 ttggttttga caaatcaaaa gtcaatgagg ttttggaaat tgaagaccgt ttccgcccag
    12721 aactcttgat cacagtgggt tacacagacg acaaattgga accaagctac cgcttgccag
    12781 tagatgaaat catcgaaaaa agataggaag aagaaaaaat gatagcaatt gattttacag
    12841 cagaagtaga aaaacgcaaa gaagacctct tggctgactt gtttagcctt ttggaaatca
    12901 actcagaacg tgatgatagc aaggctgatg cccaacatcc atttggacct ggtccagtaa
    12961 aagccttgga aaaattcctt gaaatcgcag accgtgatgg ttatccaact aagaatgtcg
    13021 ataactacgc aggacatttt gagtttggtg atggagaaga agttctcgga atctttgccc
    13081 atatggacgt ggttccagct ggtagcggtt gggatacaga tccttacaca ccaactatca
    13141 aagatggtcg cctttatgcg cgcggggctt cggacgataa gggtcctaca acagcttgtt
    13201 actatggttt gaaaatcatc aaagaattgg gtcttccaac ttctaagaaa gttcgcttca
    13261 tcgttggaac agacgaagaa tcaggctggg cagacatgga ctactacttt gagcacgtag
    13321 gacttgccaa accagatttc ggtttctcac cagatgctga atttccaatc atcaatggtg
    13381 aaaaaggaaa tatcacggaa tacctccact ttgcaggaga aaatacaggt gctgcccgtc
    13441 ttcacagctt tacaggtggt ttacgtgaaa atatggtacc agaatcagca acagcagtcg
    13501 tttcaggtga tttggctgac ttgcaagcta aactagatgc ctttgttgca gaacacaaac
    13561 ttagaggaga actccaagaa gaagctggca aatacaaggt gacgatcatt ggtaaatcag
    13621 cccacggtgc tatgcctgct tcaggtgtca atggcgcaac ttaccttgcc ctcttcctca
    13681 gccagtttgg ctttgctggt ccagccaaag actaccttga catcgcaggt aaaattctct
    13741 tgaacgatca tgagggtgaa aatctcaaga ttgctcatgt ggatgaaaag atgggtgctc
    13801 tttctatgaa tgccggagtc ttccgcttcg atgaaacaag tgctgataat accattgccc
    13861 ttaacatccg ctatccaaaa ggaacaagtc cagaacaaat caaatcaatc ctagaaaact
    13921 tgccagttgc ttctgtcagt ctatcagaac atggtcatac ccctcactat gtgccaatgg
    13981 aagatccact tgtgcaaacc ttgttgaatg tctatgaaaa acaaacaggc ctacaaggtc
    14041 atgaacaagt catcggtggt ggaacctttg gtcgcttgtt agagcgcgga gttgcctatg
    14101 gtgctatgtt cccagactca attgatacca tgcaccaagc caatgaattt attgccttgg
    14161 acgatctctt ccgagcagca gcaatttatg ccgaagctat ttacgaattg atcaaataaa
    14221 acgatagaag tctgagatct tatgcttgga cttctttttg gagggaaagt agatgtctca
    14281 aatcgaaaga atcaaacagg ctatcatggc ggattcgcag aatgccagct atacagagcg
    14341 tggcattgag cctctctttg cagcgccaaa aactgctcgc atcaatatca tcggtcaggc
    14401 tccgggactt aaaactcaag aagcaggcct ttactggaaa gataaaagtg gtgaccgctt
    14461 gcgggactgg ctaggtgtgg atgaagatac cttttacaat tcaggttatt ttgctgtttt
    14521 gcctatggat ttctactttc caggacatgg caagtcgggt gatcttccgc ctcgtacagg
    14581 ttttgcagaa aaatggcatc cgcagctctt acaggaattg cctgatattc agttaaccct
    14641 cttgattggg caatatgccc aagcctacta tttacaggag aaaatcagtg ggaaggtaac
    14701 ggagagggtg aaacactata aagactatct gccagcctat tttccgctag ttcacccatc
    14761 accacgaaat caaatctgga tggccaaaaa tccttggttt gaggcagaag tagtgccaga
    14821 tttgaaaaaa agaattaaaa ccattttata gtcaatgaaa atcaaagagc aaactaggaa
    14881 gctagtcgta ggctgctcaa agtacagctt tgaagttgca gataaaactg acgaagtcgg
    14941 taacatacgc acggtaaggc gacgctgacg tggtttgaag agattttcga agagtattag
    15001 aagaaaaaga atgaaagaaa tagcctttga cgcattttac cagctttacc aaaacgacca
    15061 gctttcttta gtggatgtga gagaagtgga tgagtttgca gctcttcatt tagaaggtgc
    15121 ccacaaccta ccgcttagtc aattggctga tagttatgat taattggaca aagatcgctt
    15181 gcattatatt atttgcaaat ctggaatgag atcggcgcgt gcttgccaat tcctattaga
    15241 acaaggttat aatgttatca atgtccaggg tggcatgtta gcctttgaag aactttaaaa
    15301 ttttgcattt ctcctacttg gtgtggactg ggtaggagag ttttattttt agataattct
    15361 tatttttaag aaaattgaaa acatttaata tttgcctcgt gatgcttttt tcagactcct
    15421 aatcgtggta tactaggtca gtattttata aatatgaagg agatttttat ggctaaaaaa
    15481 ggtaccctaa caggtttgct cctgtttgga atattttttg gtgcggggaa cttgattttt
    15541 ccgccttctc taggtgctct atctggagaa cattttcttc ctgccatcgc aggttttgtc
    15601 ttttcaggcg ttggtatcgc cgtcttgacc cttattattg gaacgctaaa tcctaaagga
    15661 tatatctacg agatttcaac gaagatagcg ccttggtttg cgactcttta cctctcagtt
    15721 ctttacttgt caatcggtcc attctttgct accccacgta ctgctacaac agcttacgaa
    15781 gtagggatta gccccctttt gtcggatgca aataaaggac ttggcttgat tgtatttacg
    15841 gttctgtatt ttgcggcagc ctatttgatt tcgcttaatc catcaaaaat cttagaccgc
    15901 attggacgta ttttaacgcc agtctttgca attttgattg ttatcttggt cgttctggga
    15961 gctatcaaat atggtggaac aagtcctcaa gctgcttcag ctgcttatca agcttctgcc
    16021 tttggtacag gtttcctaga aggttacaat accttggacg cccttgcctc agtggccttt
    16081 agcgtaatcg cagttcaaac cttgaaacaa cttggatttt caagtaagaa agaatacatt
    16141 tcaactattt gggttgttgg tatcgttgtt gcccttgcct tcagcgctct ttacatcggt
    16201 ttaggttttc ttggaaatca tttcccagta ccagctgaag cgatgaaggg tggaacacca
    16261 ggtgtttaca tcttgtcaca agccactcaa gaaatctttg gctcaacagc tcaactcttc
    16321 cttgcagcta tggttaccgt aacctgcttc acaacgactg ttggtttgat tgtgtcaaca
    16381 gctgagttct ttaatgagcg cttcccacaa atcagctaca aggtttatgc gacagccttt
    16441 accttgattg gatttgctat tgccaatttg ggtcttgatg cgattatcaa gtactcaatt
    16501 ccagtactgg ttatcttgta cccaatcacg attgctatcg ttatgattgt cattgtcaac
    16561 aaatttgttg ccctttcaaa accaggtatg cagttgacaa ttgctgtggt tacagttatt
    16621 gccattgcaa gcgtactagg aagctcgttt aaggttgagt ttcttgcaaa tcttgttagc
    16681 gttcttcctt ttgccaaggc atctctccca tggttggtgc cagccattgt tggaatcttg
    16741 ctctcattgg ttctaccaaa caagcaagaa agcgatgttt ttgaaatgga ataatcactt
    16801 agatcacttt tgtagccaag tctacaggag tgattttctt tttttatccg atgataaatg
    16861 tgttataata ggtagcgaaa gaggtgaaga aatgaatcaa acagtagaat atatcaaaga
    16921 actgacagcc atcgcatcgc caacgggctt cactcgtgaa atttcgaatt atttagtcaa
    16981 gactctagaa ggttttggtt accagccagt tcgcacagcc aagggtggtg tcaatgtgac
    17041 catcaaaggt caaaatgatg agcaacatcg ctatgtgact gcccatgtgg acacgctggg
    17101 tgctattgtt cgtgctgtca aaccagatgg tcgtcttaaa ttggaccgta tcggtggttt
    17161 tccttggaac atgattgaag gtgaaaactg taccgttcat gtggctagca caggtcaaaa
    17221 ggtatcagga accatcctca tccaccaaac ttcttgccat gtctacaagg atgcaggaac
    17281 tgcagaacgc acgcaggaca atatggaagt gcgtttggat gtaaaagtaa gcaatgaaaa
    17341 agaaactcgt gctcttggga ttgaggtcgg tgattttatc agcttcgatc cacgaactgt
    17401 tgtgactgat acaggtttta tcaagtctcg tcatttggat gataaggtca gcgcagcgat
    17461 tttgctcaac ctgcttcgta tttataagaa agagaagatt gaattgccag taacaactca
    17521 ttttgctttt tcagtttttg aagaagtagg acatggtgct aattctaaca ttcctgctca
    17581 ggtagtcgaa tatctggctg ttgatatggg agctatgggc gatggccagc aaacagatga
    17641 gtacacagtg tctatctgtg tcaaggatgc ctcaggccct tatcactatg acttccgtca
    17701 acacttggtg accttggcga aagagcaaaa tattcctttt aagctggaca tctatccatt
    17761 ttatggttcg gatgcttcag cggccatgtc cgcaggggca gaggtcaaac acgccctcct
    17821 tggcgcgggt atcgagtcta gtcattctta tgagcgtact catattgact cggtggtcgc
    17881 aacagagcgt atggttgatg cttacattaa gagcgcgttg gtagactaat atgtgtctta
    17941 tatgccagag aattgagctc atcaagaagg gagaaaatcc ctattttgtc aaagagttgg
    18001 aaacaggcta tcttgtgatt ggagatcacc agtattttgc aggctatagt ctctttctag
    18061 ccaaggaaca cgtcacggaa ttgcaccatt tagaaaagga aacaagacta cgttttctcg
    18121 aagaaatgag tctaatccaa gaggcagttg ctaaggcctt tactgctgag aaaatgaata
    18181 ttgaactttt aggaaatggc gatgcccatc ttcattggca tctgtttcca cgacggacag
    18241 gtgatatgaa tggtcacggt ctcaagggac gtggtccagt ttggtgggtt ccttttgaag
    18301 aaatgacagc agaaacctgc caagcaaaac cggatgagat caaaagatta gtcaaacgtt
    18361 tatcatcaga agtagataaa ctattagaaa taaaggagta gaaatgaaga aaagatacct
    18421 tgtcgtgaca gccttgctag ccttgagtct agcagcttgt tcacaagaaa aagcaaaaaa
    18481 tgaagatggc acagctaaga cagaacagac agccaaagct gatgggacag tcggcagtaa
    18541 gtctcaaggg gctgcccaga aaaaagcaga agtggtcaat aaaggtgatt actacagcat
    18601 ccaaggaaaa tacgacgaaa tcatcgtagc caataaacat tatcctttgt cgaaagacta
    18661 taatcccggg gaaaatccaa cagccaaggc tgagttggtc aaactcatca aagcgatgca
    18721 agaggcaggt ttccctatta gtgatcatta cagtggtttt agaagttatg aaactcagac
    18781 caagctctat caagattatg tcaaccaaga tggaaaggca gcagctgacc gttactctgc
    18841 ccgtcctggc tatagcgaac accagacagg cttggccttt gatgtgattg ggactgatgg
    18901 tgatttggtg acagaagaaa aagcagccca atggctcttg gaccatgcag ctgattatgg
    18961 ctttgttgtc cgttatctca aaggcaagga aaaggaaaca ggctatatgg ctgaggaatg
    19021 gcacctgcgt tatgtaggaa aagaagctaa agaaattgct gcaagcggtc tcagtttgga
    19081 agaatactat ggctttgaag gcggagacta cgtcgattaa tactcttcga aaatctcttc
    19141 agaccacgtc agcgtcgcct tacctactga ctgcgtcggt tctattcaca acctcaaaac
    19201 agtgttttga gctgacttcg tcagttttat ctgcaacctc aaagctgtat tttgagcagc
    19261 ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaaaaa taaacttttc
    19321 tcttgcaatt ccagataaat agtgtataat ggatgggtat gtgaaaaaca tacttgtggg
    19381 aggtaaaaat ctctaattac cgccaaaacc acaaaggagg atttaaaaat ggctaaaaaa
    19441 gtcgaaaaac ttgtaaaatt gcaaatccct gctggtaaag ctacaccagc tccaccggtt
    19501 ggacctgctc ttggtcaagc tggtatcaac atcatgggat tcacaaaaga gttcaacgct
    19561 cgtacagctg accaagctgg tatgatcatt ccagttgtta tctcagttta cgaagataaa
    19621 tcatttactt tcgttacgaa aacaccacca gctgctgttc ttttgaaaaa agctgcaggt
    19681 gttgaaaaag gatcaggtac acctaataaa actaaagttg ctacagttac tcgtgcacaa
    19741 gtacaagaaa ttgcagaaac taagatgcca gatttgaacg cagcaaacgt agagtctgca
    19801 atgcgtatga tcgaaggtac tgctcgttct atgggattca ctgttgttga ctaatcaata
    19861 acacagtaga aaatctcaca gcagtctatt agttgctttt catactaggc aagtgactga
    19921 ggcttgtact tgggtacagc aagggagctt aaggccgtag gaagagaaaa atagtagact
    19981 gaaaacccgc aagacttcat catttcgaga agtgacgtgg gagatgaaaa tcgattgaac
    20041 cacttacaag gagaatagaa aatggctaaa aaaagcaaac aacttcgtgc tgctcttgag
    20101 aaaatcgaca gcacaaaagc atacagtgta gaagaagctg tagcacttgc aaaagaaact
    20161 aactttgcaa aatttgatgc aactgtagaa gttgcttaca acttgaacat cgacgttaaa
    20221 aaagctgacc aacaaatccg tggagcaatg gtattgccaa acggtactgg taaaacttca
    20281 cgtgttcttg ttttcgcacg tggtgcaaaa gctgaagaag caaaagctgc tggtgcagac
    20341 tttgttggtg aagatgacct tgttgctaaa atcaacgacg gttggttgga cttcgacgta
    20401 gttatcgcta cacctgatat gatggctctt gttggacgtc ttggacgtgt ccttggacca
    20461 cgtaacttga tgccaaaccc taaaactggt actgtaacaa tggatgttgc taaagcagtt
    20521 gaagagtcta aaggtggtaa aatcacttac cgtgctgacc gtgcaggtaa cgttcaagca
    20581 atcatcggta aagtatcatt tgaagctgaa aaattggttg aaaactttaa agctttcaac
    20641 gaaacaatcc aaaaagcaaa accagctaca gctaaaggaa cttacgtaac aaacttgact
    20701 atcacaacta ctcaaggtgt tggtatcaaa gttgacgtaa actcacttta atcagtagtt
    20761 aaagtaatgt aaaaaagttg aagacgctat gtctcaactt tttttgatgt acgacgggca
    20821 tgttgtatag tagattgaaa ctagaatagt acacatctac ttctaaaaca ttgttagaaa
    20881 ttgatttgac tgtcctgatc gattcgtcct attcttattt cattttatta tatatttgag
    20941 gtgtaagtcc tcggtgggca cctgctaccg gtgaacccaa tagcgattcc caagtctgac
    21001 tatcgtgagg tagcggatta aaacggtctg gggatagacc gttttaagtc tgacgctgga
    21061 aataagaatc gtcagaggaa gggatagcga aatcgtggct ctacgaacag gaacgtgata
    21121 ataaggccta tatagcggat aaggtatctg gctggggcga ccactatcaa ttagtagctc
    21181 agaagtcggt acttaaacgt gctatatcaa aaccagtcct tgaaaaacgt ggactggttt
    21241 cgtgtttgga ttattacctt gaatgacatg cgttaacagt tagttgaacc gccgtatgcc
    21301 gaacggcacg tacggtggtg tgagaggggc tagagattat cccctactcg atttcgaaat
    21361 ctagtggaat gaatctggaa tagtccatcg agctttctaa tactcttcga aaatctcttc
    21421 aaaccacgtc aacgtcgcct tgccgtgcgt atggttactg acttcgtcag ttctatccac
    21481 aacctcaaaa cagtgttttg agctgactac gtcagttcta tccacaacct caaaacagtg
    21541 ttttgagctg actacgtcag ttctatccac aacctcaaaa cagtgttttg agctgacttc
    21601 gtcagttcta tccacaacct caaaacagtg ttttgagctg actacgtcag ttccatctac
    21661 aacctcaaaa cagtgttttg agcaacctga ggctagtttc ctagtttgct ctttggtttt
    21721 cattgagtat aacagattgt tagaagttgg tttaaatttc ctaatcagtt tgttcacatt
    21781 taccttcgat atattatatc ccatagttaa ggttggtcat acagatgatt atagtcatgg
    21841 agccgtaaaa cttagtgttt ctttagttga caaagatgcc atgaaaaaaa tatctgtaac
    21901 tgtaatagga tattttgaaa taaatataga tgaaaatatc accgatattc tatacgtaaa
    21961 tggtactgct attctttatc attatttacg ttctattgtt tcaatagttt cggcaattga
    22021 tagcagtgaa gcaatgttgc tacctaccat taatgtttta gagttactag ataaatctca
    22081 accttttgaa gaagaataat ttattagctc actaaattga gggtaaggaa aagtaaaatc
    22141 agtaagaaaa atgtcttgca ttatacagca accttttggg aatgagtaga tggattgaat
    22201 aaaattttat taagagtgga tgatttatct gtagattatt attggacagt tagtcttgaa
    22261 gtagtctaag aattaggtta taatcagtag aatccttgct aataatgagg aggttagttt
    22321 at
//
LOCUS       DAVLYV010000026        22299 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLYV010000026 DAVLYV010000000
VERSION     DAVLYV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.026"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..166)
                     /gene="blpI"
                     /locus_tag="VYO70_000995"
     CDS             complement(<1..166)
                     /gene="blpI"
                     /locus_tag="VYO70_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HEV1713246.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLI"
     gene            448..1035
                     /locus_tag="VYO70_000996"
     CDS             448..1035
                     /locus_tag="VYO70_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1713247.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLR"
     gene            1052..2591
                     /locus_tag="VYO70_000997"
                     /pseudo
     CDS             1052..2591
                     /locus_tag="VYO70_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
     gene            2602..3963
                     /locus_tag="VYO70_000998"
     CDS             2602..3963
                     /locus_tag="VYO70_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1713248.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVISSIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTSNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGENRI
                     PTGTEIAQVFPIITDTREVLITYYVASDYLPLLDKGQTVRLKLEKIGNHGITIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTAKKTYFDYFKDKILT
                     HSD"
     gene            4020..4175
                     /gene="blpC"
                     /locus_tag="VYO70_000999"
     CDS             4020..4175
                     /gene="blpC"
                     /locus_tag="VYO70_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1713249.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGWWEELLHETILSKFKITKA
                     LELPIQL"
     gene            complement(4217..5557)
                     /locus_tag="VYO70_001000"
     CDS             complement(4217..5557)
                     /locus_tag="VYO70_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1713250.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLFKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKAFKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(5571..6308)
                     /locus_tag="VYO70_001001"
     CDS             complement(5571..6308)
                     /locus_tag="VYO70_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1713251.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(6313..6651)
                     /locus_tag="VYO70_001002"
     CDS             complement(6313..6651)
                     /locus_tag="VYO70_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1713252.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            6928..7257
                     /locus_tag="VYO70_001003"
     CDS             6928..7257
                     /locus_tag="VYO70_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1713253.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(7425..8474)
                     /locus_tag="VYO70_001004"
     CDS             complement(7425..8474)
                     /locus_tag="VYO70_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713254.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(8471..9202)
                     /locus_tag="VYO70_001005"
     CDS             complement(8471..9202)
                     /locus_tag="VYO70_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713255.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMLRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            9270..9680
                     /locus_tag="VYO70_001006"
     CDS             9270..9680
                     /locus_tag="VYO70_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1713256.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQIAETIKNA"
     gene            9690..9977
                     /locus_tag="VYO70_001007"
     CDS             9690..9977
                     /locus_tag="VYO70_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713257.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            10139..10378
                     /locus_tag="VYO70_001008"
     CDS             10139..10378
                     /locus_tag="VYO70_001008"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713258.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHGKPTLTWFEEIFEEYYSPFHN"
     gene            complement(10712..11848)
                     /gene="dnaJ"
                     /locus_tag="VYO70_001009"
     CDS             complement(10712..11848)
                     /gene="dnaJ"
                     /locus_tag="VYO70_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1713259.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRGKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(12250..12606)
                     /locus_tag="VYO70_001010"
     CDS             complement(12250..12606)
                     /locus_tag="VYO70_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV1713260.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(12608..14431)
                     /gene="dnaK"
                     /locus_tag="VYO70_001011"
     CDS             complement(12608..14431)
                     /gene="dnaK"
                     /locus_tag="VYO70_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1713261.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAESDKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(14920..15444)
                     /gene="grpE"
                     /locus_tag="VYO70_001012"
     CDS             complement(14920..15444)
                     /gene="grpE"
                     /locus_tag="VYO70_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1713262.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(15471..16505)
                     /gene="hrcA"
                     /locus_tag="VYO70_001013"
     CDS             complement(15471..16505)
                     /gene="hrcA"
                     /locus_tag="VYO70_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1713263.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQINVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(16669..17178)
                     /locus_tag="VYO70_001014"
     CDS             complement(16669..17178)
                     /locus_tag="VYO70_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HEV1713264.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCIATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            17783..20116
                     /locus_tag="VYO70_001015"
     CDS             17783..20116
                     /locus_tag="VYO70_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1713265.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFRPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKVIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDTNGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            20129..21592
                     /locus_tag="VYO70_001016"
     CDS             20129..21592
                     /locus_tag="VYO70_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1713266.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            21592..>22299
                     /locus_tag="VYO70_001017"
     CDS             21592..>22299
                     /locus_tag="VYO70_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1713267.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLLSDNFINRVNNKSTGTSYPAINDY
                     NFNLLLIALPPLSEQQRI"
BASE COUNT         7093 a         4526 c         4106 g         6574 t
ORIGIN      
        1 aaataagtgt tgctgctccc ccagttatcg gaccagtcgc agcagctaat agtactgctc
       61 caatacaacc accgattgca gatcctaaat tgcctcttcc tccactaact atttcgagtt
      121 cttcattatc cataacagaa aattgttcca tcatttttgt attcatgaca aatactcctt
      181 ttttcttttt ttatttttgt cttgttgtaa ctttgataag tttagtatat catcgttttt
      241 taaaattttt catccagatc ttgaattgtc atcgaaacgt cttgaattag cttttttatt
      301 tcaagccacc tctaaatgtt taaaaaaaat aatttctaat cactttttta ccattcagga
      361 agttttaatg actattcaag atttcataaa atatgaactt agttttatga cataatagac
      421 ctatccacta tatgaaagga attgccaatg acttcttata aacgtacatt tgttcctcaa
      481 atagatgcga gagactgtgg tgtcgctgcc ttagcctcga ttgctaaatt ctatggttca
      541 gatttttctc tagctcactt gagagaactt gcaaagacca ataaagaagg gacgactgct
      601 cttggcattg taaaagccgc tgatgaaatg ggctttgaaa caagacctgt tcaagcagat
      661 aaaacgctct ttgacatgag tgatgtcccc tatccattta tcgttcacgt taacaaagaa
      721 ggaaaactcc aacattacta tgttgtctat caaacaaaga aagactatct gattattggt
      781 gatcctgacc cttctgtaaa aatcactaaa atgtcaaaag aacgcttttt ctatgaatgg
      841 actggagtag ctatttttct agctaccaaa cccagctatc aaccccataa agataaaaag
      901 aatggtctac taagcaagct tccttcctct gattttcaaa caaaaatctc tcattgctta
      961 cattgttctc tcaagcttat tggtcactat tatcaatata ggtggttctt actatctcca
     1021 aggaatctta gatgaataca tcccaaatca gatgaaatca actttaggaa tcatctcagt
     1081 tggtctggtt atcacctata tcctccaaca agtcatgagc ttctccagag attatctcct
     1141 aaccgttctg agccagagat taagcattga tgtgatttta tcctatattc gccatatttt
     1201 tgaacttccc atgtctttct ttgcgacacg tcgtacagga gaaatcattt cacggttcac
     1261 agatgctaac tctattatag atgccttggc ttctaccatt ctttctcttt ttctggatgt
     1321 ttctattctg agtcttgctg gcacaaaacc ctaatctctt cctcctttct cttctttcca
     1381 ttcctatata catgttcatc atcttttctt ttatgaaacc tttcgaaaaa atgaaccatg
     1441 atgtcatgca aagtaattct atggttagct ctgccattat cgaagatatc aacgggattg
     1501 aaactataaa gtcgctcacg agtgaagaaa atcgctatca aaatatagac agcgaatttg
     1561 tagattattt ggaaaaatcc tttaagctca gtaaatattc tattttacaa acgagtttaa
     1621 agcagggaac aaaattagtt ctgaatatcc ttatcctatg gtttggcgct caattagtca
     1681 tgtcaagtaa aatttctatc ggtcagctga ttacctttaa cacacttttt tcttacttta
     1741 caactcctat ggaaaatatt atcaacctcc aaaccaaact ccaatctgcg aaggtcgcta
     1801 ataaccgttt gaacgaagtc tatctagtcg aatctgaatt tcaagttcaa gaaaaccctg
     1861 ttcattcaca ttttttgatg ggcgatattg aatttgatga cctttcttat aagtatggtt
     1921 ttggacgaga taccttaaca gatattaatc tcacgattaa acaaggagat aaggttagcc
     1981 tagttggagt tagtggttct ggtaaaacaa ctttagccaa aatgattgtc aatttctttg
     2041 aaccctacaa agggcatatt tccatcaatc atcaggatat taaaaacatt gataaaaaag
     2101 tcttgcgccg tcatattaat tacctacccc aacaagccta tatctttaat ggctctattt
     2161 tggaaaactt aaccttgggc ggtaatcata tgattagtca agaagatatt ctaaaagctt
     2221 gtgaagtagc tgaaatccgt caagacattg aaagaatgcc tatgggctat caaactcagc
     2281 tctctgatgg agctggtcta tcaggaggac agaagcaacg aatcgctctc gctcgtgctc
     2341 ttttaactaa atctcctgtt ttaatactag atgaagctac tagcggtctt gatgtcttga
     2401 ctgagaaaaa agttatagat aatcttatgt ccctaactga taaaaccatt ctctttgtag
     2461 cccatcgtct cagtatagcc gaacgaacca accgtgtcat tgttcttgac caggggaaaa
     2521 tcattgaagt tggtagccac caagagttaa tgcaggcgca aggtttctac catcatctat
     2581 tcaataaata aggagaatgt catgaatcct aatcttttta gaagcgtcga gttttatcag
     2641 agacgttacc ataactatgc gacagtgtta attatacctc tttcattact atttactttc
     2701 atcttgattt tctcccttgt tgccacaaaa gaaattactg tcacttccca aggagaaatc
     2761 gcccctacaa gtgtcatttc atctattcag tcaaccagtg ataatcctat ccttgctaat
     2821 catttagtgg caaatcaagt agttgaaaaa ggggacttac tcatcaaata ctctgaaaca
     2881 atggaagaaa gtcagaaaac tgccttagca actcaattac aaagacttga gaagcaaaaa
     2941 gaaggacttg gaattttgaa acaaagctta gaaaaagcga ctgatctttt ttctggcgag
     3001 gatgaatttg gctaccataa tacctttatg aattttacta aacaatccca tgatattgaa
     3061 ctgggtatca caaagactaa cactgaggtt tcaaatcaag ctaatcttgc caatagcagt
     3121 tcatcagcta ttgaacaaga aattacaaaa gttcaacaac aaattggaga atatcaagag
     3181 ctgagagatg ctatcataaa taacagagca cgcttaccaa ctagcaatcc acaccagtca
     3241 attttgaatc gttatcttgt agcctcacaa ggacaaacac aaggaacggc agaggagcca
     3301 tttttatctc aaattaatca aagtattgca ggtcttgaat catctatcgc aagcctcaaa
     3361 attcagcaag ctggtatcgg aagtgtagca acttatgata atagtttagc aaccaaaatt
     3421 gaagtactcc gcactcagtt tttacaaaca gcctcacagc aacaactaac cgtggagaac
     3481 caattaacag aattaaaagt acaactagat caagcgacac agcgcttaga aaacaatacc
     3541 ttaaccgctc caagtaaagg tatcgttcat ctgaacagcg aatttgaagg tgaaaataga
     3601 attccaactg gtacagaaat tgctcaagta ttccctatca tcacagatac aagagaagta
     3661 ctaatcactt actacgtagc ttctgactat ctacctctac tagataaagg acaaactgta
     3721 agattaaaac tggagaagat tggaaatcac ggcattacca tcatcggcca acttcagaca
     3781 attgatcaaa ctcctaccag aacagagcaa ggcaatctct ttaaattaac cgctcttgca
     3841 aaactatcta atgaggatag taaactcatc caatatggct tacaaggtcg cgtcactagt
     3901 gtaactgcaa agaaaacata ttttgattat ttcaaagata aaattttaac ccattctgat
     3961 taattttcag ataacactct ataactattt attatcttat caaaaaggag aatcataaca
     4021 tggataagaa acaaaaccta acttcatttc aagaactaac aactaccgaa ctcaaccaaa
     4081 ttacaggtgg aggatggtgg gaagaactct tacatgaaac aattttaagt aaatttaaaa
     4141 tcacaaaagc acttgaacta cctattcagc tataaaaaca agaccgagaa acaagtactc
     4201 tcggtcttat ttttcatcat tctgtatgta tcacagtaag tacctgacga aagacttgat
     4261 tttgacaggt ggtatttaga ctggtattag gatggctttc cacaatcttc atgacggtat
     4321 agagaccaac tcctctctcc tcccctttag aactggctcc aaaggagaag atttcagaaa
     4381 tatcgatgcc ctcttctttg atggagtttt cgatgataaa ggtctcctgt gctccatttt
     4441 ttaaaaaggc gattgaaaca tgaggttgac tagcttccac actggcttca atagcattgt
     4501 cacaaaggat agacacaatg gttagaaaat caagtagact catcccctcg acctgaatct
     4561 cctcaggaac ttcgacatta aagacaatgt tcttatctct ggcttttaaa aatttccctg
     4621 ctagaagact tttgaaggct ttatcacgaa tatttaccaa tctgcccagg tcatatttat
     4681 tgttctgcaa tttctgactg gaatccttta agacggagcc atagacctct tttatctgct
     4741 ccatatcctc ctcttcaatg cccagacgta agctagtcaa gaggttggta taatcatggc
     4801 gaaaactccg tacttccttg taaagctcct ctatatgccg actatagcgt tccatatctc
     4861 tatagcgcag ggcctgctct tgttccaatc tctcatagag tttttccttc aaataggtat
     4921 ccaatttctt gataaccccc ataaaaaaga gtaggtaaaa gactaggatg agatggcgaa
     4981 cagtctttga ttgaatactt tgttcatatt caaaaaaaga cagactttcc atgactagat
     5041 agtagccacc cattatccag ttaatctgag tcagggactt ttgaaaggct ttatcgagaa
     5101 tctcctttct caagctagta aaatcgtagt ccaaccattt caaaaaagct agagaaatga
     5161 agaaattgaa aattattata cataacccag taaatgagta gccatcatat acttgccctt
     5221 gtcccaaaaa tggaagcaca aaataggaga ctcctctata aaagagattc accaatatca
     5281 ttggaaagag accataaaag aaaaggagtt ttttaggaag ccctctcaat aataagaaag
     5341 ataagcctat gccgtacaag ggttccataa aataagatag gtaaacattt cctactatat
     5401 agctaatcat cacaaaaaca aaggccaaca gtatcttaaa aagaaaggcc ttaaaaatcc
     5461 tctcgaaagt aagatcaatt ccatccacct taaagaagat gacaatttct agtccattag
     5521 taacaagtgt atacaacaat atccaagcaa tgttcataaa ttctcctagc tcagtgtaat
     5581 ttattgatgg cctcagacac ttccctgacc ttataacggg cgattagaca acttccacca
     5641 ttgggaaaga aaagcagttt ttctttctta tccaaatgca ccacatttgc aggattgatg
     5701 agaaaagagc ggtggcactg caagagacgg ggctcctgct tgaaaacctc ctctaaactc
     5761 gctgtaaatt ccagcctgtc tgtcttggta tagagaataa cacgatgggc tctgggcgac
     5821 gtttcgagat agtaaacctc tttaaaagga tactgaaatt gggcaaattt tgatttaaag
     5881 taaaagcaat cttccgccag acttttacta tcttgactat tggcatagag gagggctgtc
     5941 tcgatccgag attcaaactc ctctgctgac aaggccttat caatgtagtc caaagcagac
     6001 acttggtagc gaaaagacag gggcataaac tccgagtgag tcgtcacaaa gacaatcagg
     6061 gcataaggat cccgatcccg aatctttcta gccacttcca gtcccttcat ctcttcattt
     6121 cgaatctcaa tatccaaaaa gaatagctga tgggccccct tctcatgcac ttcagccagc
     6181 agttggtccg gcttgccaaa tacctcaaaa gagctaggaa tgatatgatg tgctttcaaa
     6241 agtttctcaa tcgtcgtttc aattctagtc tgttgggaaa aatcatcctc taaaacaaat
     6301 attctcatct tcttactctc cctctttcaa ccatttttga cgaatttctc tatagcgacg
     6361 tctggcatac ttgacagcgt attgaccttc ttctccgaga aaaaggattc tttcttgaaa
     6421 atcaatcgat tttaatttat caagattaac caaacaattt cgatgacatc tcatcaaggt
     6481 ttccccacat tggttctcaa gattacttaa attttgaagc atattaaaac tagctttttc
     6541 tgtaacaatc tgtacggtat gggctttagt tggatgtgtt tggatatagt agatatcgtc
     6601 gacgtttact ttatagactg ttttctgcgt ctgaataatc atatgtttca ttggattctc
     6661 ctcctaagaa aagaataaca gaaaagttct agcccaagcc cacccaatcc taaacagtct
     6721 ttgaaagctc ctaaaaggat tatttttcta caacatcatg atgagcttac aactatttta
     6781 tcataaaatt cactcagcgc aattcaagac atttcaatga caattaaaga tttgaataaa
     6841 aaaaaagaat taaaagtgat aaactaacag tatcaaaaca cagttgccag aagtaagact
     6901 ggcacccaga ttaaaggagg cattcccatg acagatacag accccatcaa aagagctcat
     6961 actttgatta ctgacttaaa caaagcctac caagcatgca aacaggcaag tgctgacgac
     7021 gtccgctttc aggagcaatt aaactctatt cttggttttc tagccaaggc tgaaacagtg
     7081 gataatcgat tcttgattga actagaaaaa ttttaccaga cttccagtct tctcatggga
     7141 ctcagcgctc ttgatccaga tgctccaact cgcgccgctt ggcgggccta tgaccgcttc
     7201 cactttgacc aagtcaagac caagttaata ctcaatgaaa atcaaagagc aaactaggaa
     7261 gctagccgca agctgtactt gagtacggta aggtgacgct gacgtggttt gaagagattt
     7321 tcgaagagta taagtctcta cggaccaacc attatcctgt agtaacaaaa aagaagctga
     7381 gacagactga actcaacttc ttttttagtg tcgtatcagc aatgttagtc ctgcatctga
     7441 cgttttacct gataaggcaa atacaagact agtaaaacca aaccagctcc tagtaaggct
     7501 agaagggcca gtttttcttg gaaggtaatc aacccaacaa ctaattccac aagtaaaaca
     7561 aaactggtca atcctcgaac tacctcctgt aagccttttt ccttttgccc cttgtccagt
     7621 ggaaagagtt gggtcaaata ctggtagtca aaggcatgat agagggccag caactgaaag
     7681 agcaagaggt agttaaagag aaccaccact gctgtcgcaa tccaagcttg ctcgataaaa
     7741 acctgcgcca gtacggaaag taagagaaga cgaagactga gagcaaagag gtcgccattt
     7801 cgcagataag aacgcagata gagattttgc caaatcttcc caggcacctt ctgaacagcc
     7861 tttaagataa agtccagata ggcacgacgc ttgacactgt ttgaaattcc cttgacctgc
     7921 gtaaagaggg caaagaaacg aagcaagact tgcttacgct tgctttcttg agaaataaga
     7981 tagtcccagt ccagtccagt ttcagtgaaa aatttgctgg ccttttgacg aaagtggaaa
     8041 tattttccta cccccaataa aagcacatag agcagaaaaa ctggcaagcc ataacccatt
     8101 gctaaaaaca agggcgcaaa taacagcaag aaaagggtct gtacaaagag ccaaaagact
     8161 agggaaatgc cagtttgacg cttgagatgg agcttgattt cctcttctcc aactaagaga
     8221 aagagcttgt ctggagcctc catataggtg gcaattcctc cccaaagtaa aagtaaaaca
     8281 gacgtaattc ctacaaacaa aagaataggc caatgatttt caggaaaatg ttgtaagagt
     8341 tgactgtact ggtaggctag aaagcccaac aggacaagca ggaataagac aaagtggtca
     8401 ttgagcacat agcgcagata accaagacac tccttacgaa aggcctgctt tctctttaaa
     8461 aacaagtctt tcataggtcc tcctcttttg tcagagccaa gtaaatatca ttcaaactag
     8521 cctcaggcat atcaaaggct tcgcgtagtt gcaggagatt gcctttggca cgcacctctc
     8581 ccttgtgaag aatgacaaag gcatcacaca tcttctccgc cgaatccagc acgtgggtac
     8641 tcatgagaat agacttgccc ttttgcttct ccacttccaa aagctgaatc aaatcagaaa
     8701 tagccagcgg atcaagacca aggaaaggct catccacgat gaaaagactt ggatccacca
     8761 caaaagcaca gataatcatg accttctgct tcatcccttt tgaaaaatga acagggaacc
     8821 agtctaattt ctgttccaga cggagcatct ttaacaaggg ctctactcgt tcgaaagcca
     8881 ctttttgctc aataccataa gccatagcaa ccgtttcgat atgctctctg agggtcaatt
     8941 cctcatacag actaggcgtc tcaggaatgt agccaatctg cttgcggtag ctagtcgcat
     9001 ctccttgcag agtcaggcca ttgatattga tggagccact ataaggtgcc aacagaccga
     9061 taatctcatt gatcgtcgtt gatttcccag caccattgag accaatcaaa ccgaccaact
     9121 gcccactttc aacagtaaag gacacatctt tcaaaacagg aacatgaaca tagccacctg
     9181 tcaggttttt aatttctaac atattttctc caaatctggt ataatgtagc tatattatat
     9241 caaaattcaa tacagcagag gtagatttta tgtcagattg cattttttgt aaaatcatcg
     9301 caggggaaat tcctgcttca aaagtatatg aagatgagca ggtccttgcc tttcttgata
     9361 tctctcaagt aacactagga cacaccttgg tcgtgccaaa agaacactat cgcaatcttt
     9421 tggagatgga tgctacgagc gccagccaac tctttgccca agtaccaaaa gtagctcaaa
     9481 aagtcatgaa agtcactaag gctgctggta tgaatatcat ttccaactgt gaagaagtcg
     9541 ctggtcaaac agtttttcat actcacgttc accttgtgcc tcgctacagt gctgacgatg
     9601 acctcaagat tgattttatc gcccacgaac cagactttga caaacttgct caaatcgctg
     9661 aaaccatcaa aaacgcttaa ggagttgcta tgaaattatc caacctactg ctatttgcag
     9721 gagctgcagc cggaagttat ctggttacaa aaaatcgcca aaccatcaca gatgaagtct
     9781 tgaataccac tgaccgcgtt caagctatca aggacgatgt ggatattatc caaaacagcc
     9841 tgcaaatcat taaccagcaa aaagaactta tcaaggaata ccaagaagac ttgacttaca
     9901 agtttaaggt cttggaaaag gatatccaga ctagactagc tgtgataaaa gaaatgcagg
     9961 gaactgaaga taagtaaaaa gagccccccg gctctttttt aaaacgtaaa caagttatag
    10021 tgaattgaat ttagaatagc acaccatagt ttctaaagca ttgttagaga ttactttaaa
    10081 atcctcttat caatttgttc atattctatt tcaatctatt atatatatcc catcaactat
    10141 gcatcataat ttaggtaact catactcaat aaaaatcaaa aagcaaacta gaaagctagg
    10201 cacagactgc tcaaaacacc gttttaaggt tgtggataga actgacgaag tcagctcaaa
    10261 acactgtttt gaggttgtgg atagaactga cgaagtcagt aaccataccc atggcaagcc
    10321 gacgctgacg tggtttgaag agattttcga agagtattat tcaccatttc acaactaaat
    10381 cgaagacttt ctgccgtaat gaaaacacct gaaacagctt ggtttcagct gtccggaaac
    10441 tttaaaactt aggttcaaag tttaggtatg gaacttcgaa ggaggtcact accgtccgtc
    10501 attacttagg aaagtcttaa aaaatctata aagtaaaaag agcccgacag ctctttttgt
    10561 ttatactcaa tgaaaatcaa aaagcaaact aggaaactag ccacgggctg ctcaaaacac
    10621 tgttttgagg ttgtagatac gactgacgaa gtcagtcaca tatatacgac aagccgacgc
    10681 tgacgtggtt tgaagaaatt tttgaagagt attattctcc atcaaaggca tctttaatat
    10741 ggtcaaagaa gccttttttc tttggattta ctttcaagtc accagcagcc gcgaattctt
    10801 tcaaggctac tttttggcgg tcgttcaagc ctgtcggtgt tacgacatta acagtaacgt
    10861 attggtcacc aactgcaccg ccacgaaggc tcggtgcccc cttaccacgt aggcggaatt
    10921 tcttaccagt ctgagttccc tctggaataa ccaattcaac atcaccgtga acagttggaa
    10981 tatctactgt atcaccaaga gccgcttgga caaagttgag gttgagattg tagaagatag
    11041 tcgttccttc acgttcaaac ttgtcgctag cttctacaga aactactaca tacaagtccc
    11101 cataaggtcc accgttaaag cctgcttcgc cttgaccagc gaggcgaatt tgttgacctg
    11161 tttccacacc agcagggatt ttcacatgta cgctatgagc ttgtttctca tggcctgttc
    11221 catgacaggt tgtacatgga tatttgattt cttttcctcg accgtgacag acatcacagg
    11281 ttacttggcg acgcatcata ccaagaggag tctgcgtatc gacgttaatg acaccagcgc
    11341 catgacagcg tccacaagtg actggacttg tccctggctt agcaccagag ccattacatg
    11401 tacgacagcc agcttcacga tgatacttaa cttccttctc agttccgaag atagcttctt
    11461 caaaggtcaa attgacacga tactggagat catctccttg gcgaggagcg tttggattgc
    11521 gcgaagaacc gcctccgccg aagaaacttg agaaaatatc ctcaaaacca ccgaagccac
    11581 ctgccccatt gaaaccaccg aaaccaccag ctccaccaaa accaccattg gcgcctgcag
    11641 caccatactg gtcataggca gcacgttttt ggtcgtcact caaagtctca taggcttctt
    11701 gaacttcctt gtacttgtcc tcagcaccag gctccttgtt gatatctggg tgatattttt
    11761 tggaaagctt acgataagcc tttttgattt cgtctgccga agcgtttttg gataccccca
    11821 gacgatcata aaattcagta ttgttcataa ttcaagatac aaagggcgtc aaacggacac
    11881 agccatctag gagttttgac gacggacgct tacgtcctag aaaaaactat ctttttggca
    11941 cagtccgtag cccgtgttca gttacgaaca cccttattcc tttctattga tgttctgaat
    12001 cgaaccacca tttaggtcgg gttcaattcc tttcttttat atttagtaag aaattctgaa
    12061 acccgtgttc agttacgaac actctttgtt cctttctatt gatgagacaa aacccaaaca
    12121 aatgagattc cgtccctttt tatcgaaaaa cagaggctgg gttgcaacct ctgcaaataa
    12181 gtaaattaac tttattttga ttttaaatat cctataagta cagaaatatt tatatatttt
    12241 tgtctcttct tagtatttgc ctatataatt ccaattgatc ataagaaata caactactgt
    12301 ccaaatcata ccaattatga acggtattac aaatttatat gtttgtattt ctaatgatat
    12361 catagctgat accatcgccg atacaaatgc agacaacaat actgaggtaa caaaattcct
    12421 taccgcttca tcatttttga cacaactatc atgctcagct aatataattt ctctatatct
    12481 agctaaatat tctattggaa tattatcaat aaaatctttt gatgttctat agttatcagc
    12541 ttgattacaa aaatcattat aaacttttcg tgtatttttt agatactctt catccaatac
    12601 actcatctta cttttccgta aactctccgt ctacgacgtc atcgcctgcg tttcctgttg
    12661 cttgtgcgcc ttctgctcct tcttgagctt gttgcgctgc tgcggcttgt tcgtagagtt
    12721 taacagcaag tccttgagct ttttcgttca atgcttcaag ttttgctttc atgtcgtcca
    12781 agttgttgtc ttcttgagct ttcttaaggt catcaagggc agcttgggca gcgtcacgtt
    12841 ctgcgtcgaa gcctttacct tcagtttcct tgattgtctt ttcagtcgca aagattgctt
    12901 ggtccacttc attacgaagg tctacttctt ctttacgttt cttatcggat tcagcgtttg
    12961 cttctgcatc tttcatcatg cggtcgattt cttcgtcagt caaacctgag ttcgattgga
    13021 tgacaatagt ttgttctttt tgagttccaa ggtctttggc cttaacagac acgataccgt
    13081 tcttgtcgat gtcaaatgtt acttcgattt gaggaattcc acgaggtgca gctgggatat
    13141 cagtcaattg gaagcgtcca agagtcttgt tatctgctgc cattgggcgt tcaccttgaa
    13201 gaacgtggat atcaacggct ggttggttgt ctgctgctgt tgagaagact tgtgatttag
    13261 atgttgggat tgtagtgttg cgatcgataa gttttgtaaa tactccaccc attgtttcga
    13321 taccaagtga caatggcgtt acatcaagaa ggacaacatc cttgacatca ccagtaatca
    13381 caccaccttg gatagccgca cccatagcaa ctacttcatc agggtttact gatttgtttg
    13441 gttctttacc agtttcagct ttaacagctt caacaacggc agggatacga gttgaaccac
    13501 caacaaggat aacttcgtcg atttctgaca agctcaaacc tgcatctgaa agggcttgac
    13561 gaactggaac ttttgtacgt tcaacaaggt cacgagtcaa atcatcaaat ttcgcacgag
    13621 ttaaagtcat ttccaagtga agaggtccag cctcacctgc agtgataaat ggcaagctga
    13681 tttgtgttga agttacacca gaaaggtctt tcttcgcttt ttcagccgca tctttcaaac
    13741 gttgcattgc catcttgtca gtagacaagt cgataccgtt ttctttcttg aattctgcta
    13801 ccaagtggtc aatgattttt tggtcaaagt cgtcaccacc aagtttgttg tcccctgcag
    13861 ttgacaatac gtcgaagaca ccgtcaccca attcaaggat agagacgtcg aatgtaccac
    13921 caccaaggtc aaataccaag attttttctt ctttgtcagt cttgtccaaa ccataagcaa
    13981 gagctgctgc agttggttcg ttaacaatac gttctacttc aagaccagca attttaccag
    14041 cgtcttttgt tgcttgacgt tgagcgtcgt tgaagtaagc cggaactgtg ataacagctt
    14101 tggttacttt ctcaccaagg tagtcttcag cgtagccttt caagtattga aggatcatag
    14161 ctgagatttc ttgtggagtg tattcttttc catttgcaga aactttttca gaagttccca
    14221 tcttagattt gatagagata actgtatctg ggtttgtgac tgcttgacgt tttgcagcat
    14281 caccaacgat gatttctccg tttttgaatg agactacaga tggagttgtg cggtttcctt
    14341 ctgggtttgc gatgattttg ctttcagttc cttcaagaac tgcaactgct gagtttgttg
    14401 tacctaagtc aataccgata attttagaca tgtgtttttc tccttgttag ttaattttct
    14461 atttttcttt ttgatactct tcttaagtgg cgacagtggc gacgccgtca gagcttgact
    14521 tcgtcagtct ctttgactaa actttgagcc taaggtctca aaatttgcgc aaatagcgcc
    14581 acggcgaaga gtatcgtctt tggttcgctt cgctcactat tacaaagcaa aacatatttt
    14641 atctttcttc atagtttatt gtcgtttcgg acaagttttc tattatgtaa attgcgacac
    14701 gaggagtcga aatcgatttt atttcgacga cgagttagta aggaagctag gcaaacgcca
    14761 tagcaattgc cgttttctga cgagttgctt tagctaccgt cagaattgcc gaatcgaaca
    14821 cgccctaagc tctgagtaaa aaagatagat tttcttgtgt tcatcgaaca cttcgttaat
    14881 ctcctatttt tactgcgagc tttttacggg ctttgtatct tagttataca ccactaccat
    14941 tgctgggcgt aggatgcggt catggagttt gtagcctttt tgaaagactt gggcgatggt
    15001 atctactggg tgttcatcgt ctgctgggag agtttggatg gccatatggt agttatggtc
    15061 aaattcgcca tctgctgcga tttcttcaat tccttcttct ttcaaagcgt gaatcaagct
    15121 ttcttgcacc atccccaagc ccttcttcac atcatctgtc aaaccttcaa ctgcaagtgc
    15181 acgctcaagg ttgtcaagag atggtaagat tgcttttgcc aagtcctggc tacgataacg
    15241 ttgcaagttt tgacgttctt cattggcacg gcgttggata ttttgcattt ctgcatgagc
    15301 gcgaagatat ttgttttcga actcatctgc acgttcattt gccaagtcca actcagactt
    15361 ttcaggagtt gtttcttcag ctgtttccac aacttcctct tcttgaactt cttctacttc
    15421 ttcatttttt atatcttggg ccatgttcgc ctcctttaat gatttcaatc ttaatgtact
    15481 tcgtaatgat tactgctgag gtagcggtaa aaatctgtca acttcatggt caaaacacgg
    15541 ttgaccacat tgatttgatt gattagctgt tggtaatcca gattaactgg accgataatg
    15601 gctagaattc caactccccg ataaggaatg aggaacttac tactaatcac cgctaggtca
    15661 gctaaacagg actcttgacc gtctgcaaca cgaacatttt gcatctgatc ctcacgcaac
    15721 ccctcacgaa tctccaaggc caccttttgc ggttggtcaa agaactgata ggctgctaga
    15781 ttggcaaaat tcaagagatt gaccttgccc gccatcacaa tgttttcgtt gaacatttcc
    15841 ttaaagatgt gttcaaagag atcgatgaca ttatccgttg ttgtaaagta acgctggata
    15901 atctgcggaa tctccgtccg aatcttgtag tgaatatcta aaacggtgtg accgaggaaa
    15961 cgttcctgaa tgatgctctt cagtttcagc aaatcctcct gcaagaagtt ccttggaatc
    16021 agaaactgac tagtaaccgt tcgcgactcg tctagggtaa ataccgccaa ggctgtatgt
    16081 tgccccaaaa caacgatatc aaaggctgtc aaacgttgcc tgctcggctc aacatccagt
    16141 gccactaccg tacagccact caggtctgtt agcaagttag cagcctcttg cagaatatcc
    16201 tccaatttga agaattcctg atcaaaggct ttgacaatct catatacctc attttcagcc
    16261 agtcggtcaa aatccagtga gtgtttcaca tagtactgaa aaccagcaac acttggcatc
    16321 cgaccgcttg aagtatgagc cttctcaagc aacccttgtt tttctagttc cgccatgtca
    16381 ttacgaatgg ttgcactgct agagttaata gactcttgca aggcttttga tccgacaggt
    16441 tcgtgcgttt tggtaaagat gtcaataatc agatttaaaa tatcctgctg acgctctgta
    16501 accatctcat cactcctctc tgtctgatca attagcactc aatgcatcca agtgctaact
    16561 tatgattcta ttatacaccc ttagaagaga atgtcaagac aaaaactcaa aaaattagca
    16621 ctcttttata aagagtgcta aaaatcaatc tgaagaccta gaaccttgct attcatctac
    16681 tcggtatttc ctttttagtc tgaaataacc atgaattaga taagaaatca gaaaagcata
    16741 taaaacaaaa ataagaaaga aagacataga aagatcctct ctaaacaaac tcatacaaac
    16801 aacaagacca cctaagaaag tagctataca ggaacgaagt ctagatcgca taacttccca
    16861 tttgagcccc ttactcatat agacttgatc ctctggaagt aaatcagaag acgatttacg
    16921 aagaatcgaa caagaacctg ctcctatcaa ttcccaacct ctatctctaa aatcttgcag
    16981 ttcacgctta tgctctttaa gaaatctaga atcatagata cgatagataa catcgtccgg
    17041 ttgaccttgc tcaaaataga acaaaccaaa acgactagtt ctatacctcc aacctttcaa
    17101 atgcatctca tgtaaatatt cttctttctt gtccaaatca acaatggtga aaatccgaaa
    17161 ttctactctg ctattcattg tcttacccca aaattagaaa acatgcctag cgttatttat
    17221 tagataattc tttccacttt tgactcaatc tccaaaaaat ataagaaatc tgaatcgcaa
    17281 aaactatcaa taaaacccaa tctattatga aaatcaaaaa cacttcccaa ctgaaagaac
    17341 tacctccagt gacaaacttt gagaaaaacg gtagtagagc taaaaagaga agcaaaatag
    17401 gaagcatccg cattgttaaa atccgtttgg cataaaaaat ctttatttaa acgaaaatat
    17461 tatggtaaaa tttacgccag tttttgaacg gctgatgtag atattttata ctttcaaaat
    17521 gtttaaatgt gattatttat ttttgaaaaa tagctcacca gcccgactga aagtgcttat
    17581 aaatgataat aagtcgcctg ccgaaaacag cgaaaaatag cggtgttatg cggagataat
    17641 ctgacgcgat gcgaaagtat attgcatact tattttcaac aatttagcag agtattttta
    17701 taagtgtgat ataatagaag tataatttgt tctgatagtt tatttatgga gaagtagatt
    17761 tttagaatgc ggagggttca atatggttga gtttataaag tctaagaaag aaatgagtga
    17821 ggaggatatt aaagcaaatt tcatcactcc tgctattgta tccaaaggat ggaaaaatgg
    17881 tgagcatatc gcttacgaag aatacttcac tgatggtcga attgaggtta gaggagataa
    17941 ggctcgtcgt aaagaaggaa aaaaatcaga ctattcactg tattaccaat ttggaactcg
    18001 aattgcaatt gttgaggcaa aggataataa acacagcgtt cgagcaggat tacaacaagc
    18061 tattgaatat ggagagattt tagatgttcc atttgtttat tcttcgaatg gtgatggctt
    18121 tattgaacac gaccgtatca cgagagaaga acgtgagctg gagttagacg aattccctac
    18181 tcgtgaagaa ttattttctc gtatgacgaa ggaaaaagga ttgacgtacg aaattacaga
    18241 agctatctca actccatact atacagacgc cttctcaatg aaaacgccac gctattatca
    18301 gcaaatagct atcaaccgta ctattgaaac agttgccaga ggacaaaaac gagtaatgtt
    18361 tgtgatggca acaggaacgg ggaaaacgtt catggctttt caaattattc atcgccttcg
    18421 aaaagctggt ttggctaaac gagttttatt cttagcggat agaaacatct tagtagacca
    18481 aacgatggct gaagacttta ggccattcga aaaggtaatg acgaaaatta caccaaaact
    18541 tttgactgct cctgaaaaat taaattcttt tgaaatttat ctagggcttt atcagcaact
    18601 aactggtgaa gatggaactg aaacacatta tcaaaaattt gacaaagact tctttgattt
    18661 aatcgtaatt gatgaagcgc accgtggttc agctaaggaa gacagtaact ggcgtaaggt
    18721 gattgattat ttcagttctg cgacacagat tgggatgacc gctactccta aagaaaccaa
    18781 gaatgcttcc aatacggaat actttggtga gccaatctat acttatagtt taaaacaggg
    18841 aatcgaggat ggttttttgg ctccatatcg tgttatgagg gttaatttag atgtggatgt
    18901 ggatggttat cgtccagaaa ctggaaaagt tgatactaac ggacaattaa tagaagatag
    18961 gtactacggc aggaaagatt ttgataaaac cattgtcatt gatgatagaa cgcaaagagt
    19021 tgccaagttt gtttctgatt atatgaagca aaacaatgca cgatttgata aaacaattgt
    19081 tttttgtgtt gatattgacc atgccgagcg aatgcgtgct gcacttgtaa aagagaatct
    19141 agacttagtc caagaagact atcgttatgt catgcaagta actggtgaca acgctgaagg
    19201 aaaagctcaa ctggataact ttatggatgt caattctaat tttcccgcta ttgtaacaac
    19261 gtctaaatta ttaacgacag gagttaatgc taaaacatgt cgtttgattg ttttagactc
    19321 taatatccaa tccatgactg aatttaaaca aattattggt cgtggcacac gtctttatcc
    19381 tcaaaagggg aaagaatttt ttacgattat tgattttcga aatgttacca atttgtttgc
    19441 tgaccctgat tttgatggtg agccagtgaa ggtgctggaa acaggtgcga aaacagtcag
    19501 tggttccacg cccggtttcg tagatgagga aggtgaccca gtagaaaaat atatcgttac
    19561 agacaagcag gttaccattc ttaattctac tgttcaagta ttggatgaaa acgggaaact
    19621 gattaccgaa agcctgaccg actacactcg gaagaatatc ttaggtagct acgccacttt
    19681 gaacgatttt atcacagttt ggcatacggc ggataaaaag aagcttatct tagacgaact
    19741 ttataaaaaa ggagtttatc tagatgctat tcgagagtcg gagggaatat cagaacaaga
    19801 aatcgatgat tttgatttac tcctaaaact tgcctatggt caaaaagaat taaccaaaac
    19861 ggaacgtatc aataaactca aacaaagcgg atatttatat aaatatagtg aggaagcgcg
    19921 tgctgttttg gaaattttac tgaacaaata catggataaa ggtattggag aactcgaaag
    19981 cattgaaaca ttaaaacttc cagaatttca gatatatggt ggaaccttca aaatcatcaa
    20041 tacttatttt ggagataaaa aacgatattt acaagcaatt aaagaattgg agcaagagct
    20101 atttacagta gcttaatgaa aggaaagtat gtcaattaca tcatttgtaa aaagaattca
    20161 agatatcact cgaaacgatg ctggtgttaa tggtgatgct caacgtattg agcaaatgtc
    20221 ttggttatta ttcttaaaaa tttatgatag ccgtgaaatg gtttgggaat tagaagaaga
    20281 cgagtatgag tcaattatcc cagaggaatt aaaatggcga aattgggctc atgctcaaaa
    20341 tggggaacgg gtattgacag gcgatgaatt acttgatttt gtcaataaca agttattcaa
    20401 agagttgaaa gagcttgaaa taacttcaaa tatgcctatt cgaaaaacga ttgttaaatc
    20461 agcttttgaa gatgcgaaca actatatgaa aaatggcgtc ttgttacgcc aagtcatcaa
    20521 tgttattgat gaagttgatt tcaatagccc tgaagatcgt cattcgttta atgatattta
    20581 cgaaaaaatt cttaaagata ttcaaaatgc tgggaactca ggagaatttt atacgccacg
    20641 tgcagcgact gattttattg ccgaagttct tgacccaaaa cttggagaat caatggcaga
    20701 ccttgcttgc ggaacaggag gcttcttgac ttcgactctg aaccgtttaa gtagtcaacg
    20761 taaaactagt gaagatacca aaaaatataa tacagctgtt tttggtattg aaaagaaagc
    20821 atttcctcat cttttagcag ttacaaatct gtttcttcac gaaattgatg accctaaaat
    20881 tgttcatgga aatactttgg agaaaaatgt tcgtgaatat acggatgatg aaaaatttga
    20941 cattattatg atgaatccac cttttggagg atcagaatta gaaacaataa aaaataactt
    21001 tccagcagaa ttacggagtt ctgaaacagc tgatttattt atggctgtca ttatgtatcg
    21061 tttaaaagaa aatggtcgtg ttggagttat tttacctgat ggttttctat ttggtgaagg
    21121 tgtaaaaact cgcttgaaac aaaaactggt agatgagttc aacttgcata cgattattag
    21181 gttgcctcat agtgtctttg caccgtatac aggaatccat acgaacattc ttttctttga
    21241 taaaacaaag aaaacagaag aaacttggtt ttatcgttta gatatgccag atggttataa
    21301 aaatttctcg aaaactaagc cgatgaagtc agaacacttc aatcctgttc gtgactggtg
    21361 ggaaaatcgt gaagagattc tggaaggtaa gttctacaaa tctaaatcat ttacacctag
    21421 tgaattggct gagttgaatt ataatttaga ccagtgtggc tttccaaaag aggaagagga
    21481 aatcttaaat ccctttgagt tgattcagaa ttatcaagcg gaaagagcaa ctttaaatca
    21541 taagattgat aatgtattag ctgatatttt gcagttgttg gaggacaaat aatgacacca
    21601 gaacaactta aagcaagtat tctccaaaga gcgatggaag ggaaattagt gccgcaaaat
    21661 cccaatgacg aacctgcaag tgaattatta aagagaatta aagctgaaaa agaaaaactt
    21721 atcagtgaag gaaaaatcaa acgagataaa aaggaaactg agatatttcg tggtgatgat
    21781 gggaaacatt atgggaagtt tgctgatgga agtactcaag aaattgatgt tccttatgat
    21841 attcctgata cttgggagtg ggtgaggata aaatcaattt attggaattt tgggcaaaat
    21901 aagccagaga aatcctttag gtatatagat acgtctagta ttgatagaaa aaagaacata
    21961 atcaactaca aaaatctaca atatctttca cctgaacaag cgccttcccg tgctagaaaa
    22021 ttagtttcgc agaatagtgt cttattttca acagttagac catatctaaa aaatattgct
    22081 gtagttagag aacttaaaga gtatttgata gctagtacag catttattgt tttggatact
    22141 ttacttaacg aaacatattt gaaatattat ttattatcag ataattttat caatcgtgtt
    22201 aataataaat ctactggaac aagttatcct gcaatcaatg attataattt taatctatta
    22261 ttaattgctc tccccccact atccgaacaa caacgaata
//
LOCUS       DAVLYV010000027        21440 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLYV010000027 DAVLYV010000000
VERSION     DAVLYV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21440
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.027"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..495
                     /locus_tag="VYO70_001018"
                     /pseudo
     CDS             1..495
                     /locus_tag="VYO70_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(488..655)
                     /locus_tag="VYO70_001019"
     CDS             complement(488..655)
                     /locus_tag="VYO70_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1713268.1"
                     /translation="MKKRILSAGLTFTSALLLAACDQSGSDTKTYSSTFSGNPTTFNY
                     LLDYYADNIVN"
     gene            complement(820..2427)
                     /locus_tag="VYO70_001020"
     CDS             complement(820..2427)
                     /locus_tag="VYO70_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1713269.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIATIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPMQEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLIRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2580..4064
                     /locus_tag="VYO70_001021"
     CDS             2580..4064
                     /locus_tag="VYO70_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1713270.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            4159..4641
                     /locus_tag="VYO70_001022"
     CDS             4159..4641
                     /locus_tag="VYO70_001022"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1713271.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
     gene            complement(4708..5043)
                     /locus_tag="VYO70_001023"
                     /pseudo
     CDS             complement(4708..5043)
                     /locus_tag="VYO70_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(5504..7609)
                     /locus_tag="VYO70_001024"
     CDS             complement(5504..7609)
                     /locus_tag="VYO70_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1713272.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQNKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLNPFFRPELLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKV"
     gene            complement(8218..9198)
                     /locus_tag="VYO70_001025"
     CDS             complement(8218..9198)
                     /locus_tag="VYO70_001025"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1713273.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(9200..11452)
                     /gene="pbpX"
                     /locus_tag="VYO70_001026"
     CDS             complement(9200..11452)
                     /gene="pbpX"
                     /locus_tag="VYO70_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1713274.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(11456..11773)
                     /gene="ftsL"
                     /locus_tag="VYO70_001027"
     CDS             complement(11456..11773)
                     /gene="ftsL"
                     /locus_tag="VYO70_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1713275.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(11785..12735)
                     /gene="rsmH"
                     /locus_tag="VYO70_001028"
     CDS             complement(11785..12735)
                     /gene="rsmH"
                     /locus_tag="VYO70_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1713276.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            12901..13095
                     /locus_tag="VYO70_001029"
     CDS             12901..13095
                     /locus_tag="VYO70_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713277.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEDDF"
     gene            13111..13653
                     /locus_tag="VYO70_001030"
     CDS             13111..13653
                     /locus_tag="VYO70_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV1713278.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIILFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITAIDPDMLSEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLRILIGEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            13664..13906
                     /locus_tag="VYO70_001031"
     CDS             13664..13906
                     /locus_tag="VYO70_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713279.1"
                     /translation="MKKEKRQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            13917..14252
                     /locus_tag="VYO70_001032"
     CDS             13917..14252
                     /locus_tag="VYO70_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713280.1"
                     /translation="MKSLARLLIIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(14362..15363)
                     /locus_tag="VYO70_001033"
     CDS             complement(14362..15363)
                     /locus_tag="VYO70_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1713281.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(15423..17324)
                     /locus_tag="VYO70_001034"
     CDS             complement(15423..17324)
                     /locus_tag="VYO70_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HEV1713282.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYHFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     RRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGMHY
                     IEGAYWSAEPDLPYLHRRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDIGVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(17346..17639)
                     /locus_tag="VYO70_001035"
     CDS             complement(17346..17639)
                     /locus_tag="VYO70_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1713283.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(17639..18457)
                     /locus_tag="VYO70_001036"
     CDS             complement(17639..18457)
                     /locus_tag="VYO70_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1713284.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEVFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVAGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(18444..19223)
                     /locus_tag="VYO70_001037"
     CDS             complement(18444..19223)
                     /locus_tag="VYO70_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1713285.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(19238..19729)
                     /locus_tag="VYO70_001038"
     CDS             complement(19238..19729)
                     /locus_tag="VYO70_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1713286.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKNVKDALTLVEGGVPIKE
                     INIGNIHNATGKEQVTRSIFLGEEDKVALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(19740..20930)
                     /locus_tag="VYO70_001039"
     CDS             complement(19740..20930)
                     /locus_tag="VYO70_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV1713287.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(20942..21370)
                     /locus_tag="VYO70_001040"
     CDS             complement(20942..21370)
                     /locus_tag="VYO70_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1713288.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEDFELGI"
BASE COUNT         6227 a         4861 c         3865 g         6487 t
ORIGIN      
        1 ttatttctta agaattttaa tagtttaaag cacctagcac ctgtttagat tgatgaaaca
       61 ggattcgata cttattttta tcgagaatat ggtcgctcat taaaaggtca gttaagaaga
      121 ggcaaagtat ctggaagaag atatcagagg atttctttgg ttgcaggtct aacaaatggt
      181 gaattaatcg ctccaatgac ttacgaagag atgatgacga gcgacttttt tgaagtatgg
      241 tttcagaagt ttttcttacc aacattaacc acaccatcgg ttattattat ggataatgca
      301 agattccata gaatggggaa gctagaactt ttatgcgaag agtttgggca taaactttta
      361 cctcttcctc cctactcacc tgagtacaat cctattgaga aaacatgggc tcatatcaaa
      421 aagcacctca aaaaggtatt accaagttgc aatacctttt atgaggctct tttgtcttgt
      481 tcttgtttca attgactata ttatcagcgt aatagtctaa tagatagtta aatgtagttg
      541 gatttccact aaaggttgat gagtaagttt ttgtatctga acctgattgg tcgcaagcag
      601 ctaaaagcaa agcagatgta aaagtcaggc ctgcactaag gatacgcttc tttatgttcg
      661 tcttctttct ccttaatagt gggaatttgt aaagttaatt gaatttcgag aatgaaggtt
      721 tataaacctt ggttataaaa aacaaaggat ttctgccttt tatacagtcc tcccttgttt
      781 ttatacgatt tcaattttaa atttttctgc aaaaaatatt tatagtaatt ccacacagaa
      841 agcatcccat ggaactaaga tttgtttttc aaagacttct tgagctaggg tgttttcaat
      901 caagacagat ttgacttttc cttctactgt caagtcttgc tcttcattgg acaagttagc
      961 cacaactagg aagcgacggt cgccatcctt acgtatataa gcaaagacct tatcagccgt
     1021 atcaagcaat tcaaagtcag ctcgaattag ccagctattc tccttgcgaa tttggaccag
     1081 tttctgatag gtatagaaaa tagaatctgg atttgccagc gcttcttgga cgttgatcat
     1141 ctcgtaattt ggattaactg ccaaccaagg ttgacctgtt gagaaaccag cgtttttgct
     1201 ctcgtcccat tgcatagggg tacgggcatt gtcacgtcca ataacacgga tactgtccat
     1261 gatttcttgc atcggaacac ctttttcaag agcctcacgc gcatagttga gagattcaat
     1321 atcttctact tgatctagtg tttcaaacgg atagttggtc atcccaatct cctcaccttg
     1381 gtagatataa ggagttcctc tcatgagatg aagtaagatt gcaaaggctt tggcagattt
     1441 ttcgcggtat tcttggtcat ttccccagat tgagacaata cgagggaggt catggttgtt
     1501 ccagaagagg gaattccagc cgtcctcaac tcctaactct gtctgccatt tgttgaagat
     1561 ttcttttaac ttagcgatat tcagctcttt ttgatagtgc catttaggct gaccttcctg
     1621 atactgaaga ccgatatgtt caaactggaa gaccatagac aattcttgcc cctttggatc
     1681 agagtagagc ttggcaatct ctggcgttgc tccccaagtc tcccctactg tcaagagatc
     1741 cttatctcca aaggtcgcct gattcatttc cttgagatag ggatggagca taggaccatt
     1801 attgactacc ttctcgtcag gaattttgcc aatcatgtca ataacatcca tacggaaacc
     1861 accaatacct ttatcaatcc agaagttcat catctcataa attttctggc gaagtttttc
     1921 attttcccag ttgagatccg gctgtttttt gctgaaaaag tggagatagt attgacctga
     1981 cttttcatcg tattcccaag cagacccact aaagatagaa tctaggtcat tgggttcatc
     2041 gcgccagata tagtagtctc gctcagggct gtcagtattt tcacaggctt cgacaaacca
     2101 agcatgttca tctgaggtat gattaaccac caagtccatg atgatacgaa tgtcacgctt
     2161 cttagcttcc gcaatcagtt gatccatatc ctccatggtt ccaaaaatcg ttgcaatcgc
     2221 ttgataatca gcaatatcgt agccattatc atccataggg ctgtcataaa cgggagaaag
     2281 ccaaatcgct gtgattccta gcttagctag atagtccaac ttactggtaa tacctggcaa
     2341 atcaccaact ccatctccat tactatccat aaaactcttt ggatagactt gatagactac
     2401 ggcattatgc caccattttt cttgcatctt cttacctcat tattttaata atctatagta
     2461 tatgatagca ctttttaaaa ttttgtgcaa gcgtttgctt aatcttttta atttcttttt
     2521 tagctccgta gtttcctaac ttccaatttt ttattataat agagtccaga ggtaaaaaaa
     2581 tgaaaaaaca agcttttagt tctgaacaat atttgaattt acaacgcgac catattttgg
     2641 agcgcattaa ccaatttgac ggcaagctct acttagagtt tggcggtaaa atgttagaag
     2701 atttccacgc tgctcgtgtc cttcctggtt atgaacctga caacaaaatc aagctcttgc
     2761 aagaattgaa agagcaggtt gaggttgtga ttgccattaa tgctagcaac attgaacatt
     2821 ccaaagcacg tggcgactta ggcatttctt atgaccaaga agttcttcgt ttgattgaca
     2881 aattcaatga attagggatt tttgttggtt ccgttgtcat tacacaatac gctggccaac
     2941 ccgctgcaga tgccttccgc aatcaacttg agaaaaacgg aattgattct tatcttcatt
     3001 atccaatcaa aggatatccg acggatatgg atcacatcat ttccccagaa ggcatgggca
     3061 aaaacgacta catcaaaacc agtcgcaact tgattgtcgt aaccgctcct ggacccggtt
     3121 ctggaaaatt ggcaacgtgt atgtccaata tgtaccacga ccaaatcaat ggcatcaaat
     3181 ctggctacgc taaatttgaa accttccctg tttggaatct tccccttcat catccagtta
     3241 acttggctta tgaagctgcc acagctgacc ttgatgatgt caacatgatt gaccccttcc
     3301 atcttcaaac ctatggagaa accactgtca actacaaccg tgatatcgaa atcttcccag
     3361 tgctcaagcg catgttggaa cgtattctcg gaaaatcacc atacgcttca ccgacagata
     3421 tgggtgtcaa catggttggt ttcgctatta cagatgacga ggctgctgtc gaagcttcta
     3481 aacaagaaat catccgccgt tactatcaaa cagttcttga cttcaaagct gaaaaagttg
     3541 gcgaagctgc cgtcaagaaa attgagttgc tcatgaacga cctcggtatc acacctgcag
     3601 accgtaaggt tgctgtcgtt gcgcgccaaa aagcagaaga aactggtgga ccagccctag
     3661 cctttgaatt gccaaatggg gaaatcgtca ctggtaagaa ctcagaactc tttggcccta
     3721 cagccgctgc cttgatcaac gccatcaaaa aatcagctga tatcgctaaa gaagtaaaat
     3781 taatcgagcc ggaagttgtt aagccaatcc aaggtcttaa aatcgatcat ctcggtagcc
     3841 gcaatccacg ccttcattca aatgaaatcc tgattgcact tgctatcaca gctacagaaa
     3901 atcctgatgc tgcccgcgct atggaagaac tcggcaacct caaaggaagc gaagcccact
     3961 caaccatcat cttgactgat gaagacaaga atgtccttcg taaactgggt atcaacgtaa
     4021 cctttgaccc atactaccaa tacgaccgct tatatcgtaa gtaaagattt caacctctcc
     4081 agcctcggag aggttttctc tctgtctcag gagcaacctt tatgatataa tgagagaaga
     4141 aaactgaaag gatttaccat gtcaaaagaa gttattgtcg aaagttttga acttgaccac
     4201 accattgtca aagcacccta tgttcgcttg attggggaag aaacaggacc aaaaggagac
     4261 atcatctcca attatgatat tcgcttggtg caaccaaacg aagactctat ccctactgcc
     4321 ggccttcaca ctatcgagca cctcttagcc aaactcatcc gtacccgcat tgacggcatg
     4381 attgactgtt caccatttgg ttgccgcaca ggcttccaca tgattatgtg gggacgtcac
     4441 accagtgcta aaatcgcagc tgttatcaag gattcgctca aggaaatcgc tgaaactact
     4501 acttgggaag atgtcccagg gacaaccatc gaatcttgcg gaaactacaa ggaccacagc
     4561 ctcttctctg ctaaagaatg ggcaaaactc atcttggaac aagggatttc agatgatgcc
     4621 tttgaacgtc atgtgattta aaatcgagta ggggataatc tctagcccct ctcacaccac
     4681 cgtacgtgcc gtccggcata cggcggttca actaactttt aacgcatgtc gttcaaggta
     4741 ataatccaaa cacgaaacca gtccacgttt ttccaggact ggttttgata tagcacgttt
     4801 aagtaccgac ttctgagcta ctaattgata atggtcgccc cagccagata ccttatctgc
     4861 tatccattta ggaactccta acttaagcaa tccccataat cgtctcgatt tcttcttcca
     4921 ttgcttccag ataatcactc gtaggcgagt acgcaagcgc tcatctatgc tggcgactat
     4981 acttttcata tttcccaatg agaaatagtt tatccatcct cgaatgaaca aattcagttg
     5041 ctctcgtgat ttacgcatat aggttacgac tacctttttg aactttagag tttgagaccc
     5101 ccttatccgc tatatacgcc ttactatcac gttcctgttc gtaaagccac gatttcgcta
     5161 tcccttcctc tgacgattct tatttccagc gtcagactta aaacggtcta tccccagacc
     5221 gttttaatcc actacctcac gatagtcagg cttgggaatc gctattgggt tcaccggtag
     5281 tgggtgccca ccgaggactt acacctcaga tattcagata tatagtaaaa tgaaataaga
     5341 acaggacaaa tcgttcagga cagtcaaatc gatttctaac aatgttttag aagtagatgt
     5401 gtactattct agtttcaacc tactatacga catgcccgtc atacctcaaa aggaaccagt
     5461 ttttttcagc tggctccttc ttttttatcc tcaaaattct gtcttagact ttctcacgaa
     5521 taaccaaaac gccatcttcc atatctgctt ccagatgttt agcatctaaa tgatccaagt
     5581 ggaagtctgt caccttatca cgaatttctt gttcaaccac gcgacggaga ggacgaaccc
     5641 ccatgacttc gtcgtaacct tcttctgtga tataatcttt agccgcttga ctaactacca
     5701 agtcaatgtc tttcttagcc aaggtttggt taacttcagc caacatcaaa tctacaatct
     5761 tagaaagatc ttccttagtc aagtgtgaga actcgatgac tgcattaaag cggttgagga
     5821 gttctggacg gaagaagggg ttcaaacggt ccatcaattc tggtttatcc gcatcttctg
     5881 tcaagttggc ttcatagcca aatccagcat ttgaggtcgc aataatgaca gtgttcttga
     5941 agtttactgt atttccttga ccatctgtca aacgaccatc atctagaact tggaggagaa
     6001 gggtaataac ttgagggtca gccttttcaa tttcatccaa gagaatgata gagtatggat
     6061 tgcgacgaac acgttctgtt aaggtattgc tattgtcatc ataacccaca tagcctgctg
     6121 ttgtaccaat tagcttagaa acagctgtgc ggtcactgta ttcagacata tccaaacgga
     6181 taatcgcatc ctgggttcca aacatatcga gtgccaattg cttagcaagc tccgtcttac
     6241 caaccccagt agaccctaca aagaggaagc tgccgattgg gcgatttcct tcatcaaaac
     6301 cagcacggtt acgacggata gcacgagcta cagcttctac ggccttatct tgaccaatca
     6361 ccttgttttg caagcgatga gccatatctt tcaaacgttc gatatctgaa gctcccattt
     6421 gcgatactgg gatacctgtc attcgttcca cagattcagc cacatcgttg acacttgcag
     6481 tcactttcat atcttctgtg tggttttcga ttttcctttc caattctgca atgcgtgttt
     6541 tatagtttag agctgcttca aaatcttctg cttcaactgc tttttcttgc ttgtcttttt
     6601 ccgtttcgat ttctcgttca acagcatgca catctgttac tggatgttga gccgccaagt
     6661 gagcagccgt tacatcgaca aggtcaatag ccttatctgg caagctacgt tgaggaatgt
     6721 attgaacaga ataatccacc gctgctttca agacttcgtc tggcaagatg acattgtggt
     6781 gttgttgata gaggtcacga atgccttgaa gaattttaaa agtattctct gctgaaggag
     6841 cattgacctt cacttcgttg aaacgacgag caagagcagc attcttcaag atggtgttac
     6901 ggtattcgtc ttgagttgtt gccccaatca ctgtcaattc tccacgagag agagctggct
     6961 tgagaatatc cgcaagccct ttagaaccac tgtctccacc agtgctacca gcaccaagaa
     7021 tttggtgaat ttcatcaaag aagaggataa tattccctgc ttctttcact tcattgacta
     7081 agttttggac attttcttca aagctaccac ggtattgagt accagcctca agacctgaga
     7141 tatcaataga aataatttcc ttgttcttga tagcagcagg aacatctccg ttcacaatgg
     7201 cttgcgctag accttcgaca actgctgtct taccaacacc tgcatctccg accaaaacag
     7261 gattgttctt ggtgcggcgt gagaggattt cagatgtttc ttgaatttcc ttgtttcgtc
     7321 cgataacagg atccaacttg ccctcacgcg cttctgctgt caagtttcga cctagttttg
     7381 caaggacacc gtcttgtttc atacctgatg cctgttgtgg catttgcaca tcagtttctg
     7441 catttcctgg taattgacca gtcgcacgat agtgagcaaa ttcctcaggt gtgacttcgc
     7501 gtccattaat taagtaacgg cgattttcag aactatatcc tcgcatacca cccatcaatt
     7561 ggttaaataa atcatccatg ttattaaaat tattaaagtt gttattcata ttctttacct
     7621 ctttttgttt acttagttat gattactgat attgactatc tttgaccatt gttttaaaaa
     7681 atttagacta gttaaaccgc taccctacgt actatttctg gtttttcttt ttcaagcagg
     7741 agtagaccat ctttacttct gaagatttct agatgaaaca tagacccacc tctttctatt
     7801 ttactttaaa taagtgttct agtaaggctt tcatttacct tacgttcata atatactaca
     7861 ttggtcagaa aaggtcaaga gttttggctt taactgacca atattttttt acactcttcg
     7921 aaaacctctt caaaccacat cagcttctcc ttgtctacaa cctcaaaaca gtgttttgag
     7981 ctgacttcgt cagttctatc aaccatctca aaactgtata gtaaaatgaa ataagaacag
     8041 gacaaatcaa tcaggacagt caaatcgatt tctaaaaatg ttttagaagt agaggtgtac
     8101 tattctagtt tcaatctatt atattttgag cagcctaaag ttagctttct agtttgttct
     8161 ttgattttca ttgtgtattt aaacacaaaa acaccctgaa acatcagggt gccattctta
     8221 catcaaatac aaaattgcaa gggtcaggag acttgctaga agcccaactc cccaaaagaa
     8281 gaagtcaacc ttccactcgc tccaaggatt tcctttacca gacaatcccc caagctcaaa
     8341 atggtgatgt acaggcgtca tacggaaaat acgtttacca cctgtcagtt tgaaataact
     8401 gacttgcatc ataacagaag ttgtttcaaa aacatataca attccgataa tcaagagagt
     8461 ccattcttgg tggagagcca tagagatagc tgccagcatc ccacctaggg ccaaacttcc
     8521 cacatcaccc ataaagacct tagcaggctt atggttaaag atgaaaaaac tgagcaaacc
     8581 accaatcatg gcaagaatca ctagaagaat atccatctga ccttgcacat aggcaataac
     8641 tccataggca gacaaactaa tcacaacgga aatactagct aaaccgtcaa caccgtctgt
     8701 caagtttact gcgtttgaaa aaccgactag ccagaaaaga gcgaagacaa tatagaaaat
     8761 ccctagatgc acttgataac caaagacaga aagcatatcg ccaccgcgct catagaaaag
     8821 atagaagata actccaccta gaagctgaag agctaatttt tgcttaggat taagcccctc
     8881 attgatttta cgaaagacct tgagaaagtc atctaaaaat ccgaccaagc catacaagac
     8941 caagatgaac aaaatcattc ccatattatt gctgaattgg ctactaaata gggcgaaaaa
     9001 gaaagcaacc aaaacagaag taatcaagaa aaccaaacct cccattgtag gagtcccagc
     9061 ttttgcctga tgctgtttga catcctcatg catctgctgg cctgtaattt gcgcctttct
     9121 ataaaattgg ataaaggccg gaattcctac taaagttagt aaaaatgtca caattccagc
     9181 actgatggaa ataaacatat tagtctccta aagttaatgt aattttttta atgtccttga
     9241 tagctgtgtt agcacgaaca tcttgcttct gcacagtaga gcccgagcct tgaaattcaa
     9301 gttctatatt gagccactta gcaagggtct cagcagtctc ctttgtccaa ccatacatat
     9361 ctggaacctc ctctgcttta tcagataaga taaggacttg ctgattcggg gcaagattct
     9421 tcccttcttc agcagaactg tttttaatct tcgttcctgt tcccacaacg atgggttgta
     9481 caagattgcg acgcaattct tctgctaaat caccaggtga aatatccttg acactaggca
     9541 taggataagg actttgttga cttacttgct ccaaagcttt agctgttgtt tgaagattga
     9601 gagagtcttt catagctgaa gcccgctcca agataggatt ggcaaattct cccaactgaa
     9661 tacctgaata atgttcaggt tgttggaccg tcacatacaa gataaaatca ggattttcag
     9721 ccggactcat cgatacagcc gagaaaatat agtcggttac cccgactaaa taaccaccat
     9781 ttttctcgtc agcaatctga gccgtaccag acttgagggc tacattttgc ccaggaacag
     9841 ttacagttgg cttgcctgtg ctgtggttat acatggttcc ataaaccgga tccgtcccta
     9901 ccaaaaccat gttagtccga gttagactag ctgcatcttt agaaacagga tttcccacaa
     9961 tttctttttg agatttccga gcagtttgat catttggatc ataaatggca ctaataaatt
    10021 taggctccag catgacaccg tcattagcaa tagctgtaaa ggcacgaatc atttgtgtct
    10081 gggtcactga aatcccttgt ccaaatgagc tttgcgcaat gttgacaata ttatccgcag
    10141 gaagctgacc agcatactca tccgtcaaac cgaaacgggt cggaactcca aatttaaaac
    10201 gattaagata atcaagccag gtagcatctc ccatcttttg ctcaaggaga gtcatcccaa
    10261 cgttacttga gtgtgcaaaa ccttgagaaa aagtcatcat tctgccacca gtcaatcctt
    10321 cattaacgtc ccaatctcga atcgtggcat ctgcaatttt taactcacta ctattaaaga
    10381 cttctcctcc tggaaaggta ttattatcaa tagcagcagc caacatcatc actttcatag
    10441 tggaacctgg ctcatagtta ctttggtaaa ggatatcacg ccaaacaaag tcctctgtaa
    10501 tgccttcttt tgtatctgca tcaaaggtcg gtcgttgcgt tgttgccaga atttcccctg
    10561 ttttagcact gaccaaagtc gctgtcatgt actttccttt taccttctct tgaaaagcat
    10621 ccatctgggt ttccataaag gactggaggg ggctggaaat ggttgtataa acatccttac
    10681 cgtccatcgt tcgttgggaa acttgttctg ttccgggtac aatattaccc agacgatcct
    10741 tttcataggt aataatgccg tctgtccctg caagaatact gttcaaggaa ctctccattc
    10801 cagaggttcc cagcaagctc ttgcttccat cttcattttc atggagctga gctagaccga
    10861 taaaactaga agcaaattgt ccgtttgggt aactacgatt gggactggtt gtaaaatcaa
    10921 tccccttgac ctctgcagct tccaattctt ttttgataga catcatattg gcataggtaa
    10981 tcccatttcc ctttgctcca aaggaaactt gcttgagatt aggttgcgag agttgctctc
    11041 ttacatagga ttcttccatg tccagatact tatgaaagac ctctgcaacc ttgttaaatt
    11101 gtgttttttc tacgtaaaga atcttacccg ttgctgactt atagttctca tcaatgaccg
    11161 catagacatt ataggaggtt gcatcctcag caatcgggac tccatttcgg tcataaatag
    11221 tcccacgttt ggcaggaact gtacgggtgg tttgatgaac cttcttagct tccttcgcta
    11281 aatctgttcc aaagcgagtg cctgtcccaa taatgaccgc aaaattgact aaaaaaatgg
    11341 caaaaacaaa gacagataat aaactcagac tttttccaac tctgcgtctg ttttcagccg
    11401 gcgatttccg atttttggtc gcataacgga ttactctttt tgtccacttc atatcttact
    11461 ccgctattct aatattttca ttgtttaatt gcaaatcgtg tgaattggca atttctttca
    11521 aacgttctgc acgtaatagt tcattgacct cttgcttggc atcgtccaat tcggtcttct
    11581 tttcctctat ctgcgcattg atttttgtca aatcattctg cacttgcaag agcttggtct
    11641 gcataaaaat aatactaatg gctacaataa gagtggttac agcaatggaa aagtaaaaag
    11701 ctttttccac acgcgaaaac cgtttaagtt gcatctgtag tatttgacct gttttttcca
    11761 ttttttctgc catctttttc cctcttactt gtgaattttt ctggccacgc gcaacttggc
    11821 tgagtgcgag cggttattgg cttctaactc ttccgcactt ggcaagattg gcttacggga
    11881 caccaattcc atcttgggct tgagatcatc tgggatgaaa ggcaagcctt ttggaacttc
    11941 aactgttgaa gcttccttga acaattgctt agtcaagcgg tcttctaagg aatgaaaggt
    12001 aatcactgaa attctaccat ccagagccaa catatccata gcctgctgga tggactcatc
    12061 tgccgccccc agttcatcat tgacttcaat tcgaatagcc tggaaaatct gcttagcagg
    12121 atgccccttc ttcttgagtt ccttggcagg tttgaccaac ttgataatct ctgctaactc
    12181 agtcgttgtc tcaatcggct tcacttcacg cgcttgctca atcttacgcg caatctgttt
    12241 agagaatttg tcctctccat acttgaagaa aatacgaacc aagtcatgat agtcataatt
    12301 gttcaccact tcataggctg tcaggctagc atcctgattc atccgcatgt ccagtggcgc
    12361 atccttttta taagaaaaac cacgctcacg ctggtctaat tgaggactag acactcccaa
    12421 gtcataacaa attccatcaa tttcctgaac accagcttcg cgcaaacgtg cctgtaaatg
    12481 acggaagttg tccttgataa aggtcaccat tcccttctca atgtaaggtg ccaagcgttt
    12541 ttgcgcattg tcaatggcat tctgatcctg gtcaaaggca tagagatggc ctttttcact
    12601 taatttactt aataaatact cgctatggcc tgctccaccc aaagtcgcat caacgtagat
    12661 accgtcaggt tttacgtcaa gcatatcaat cgtttcgtgg agtaagaccg ttacatgatg
    12721 aaattctttt gtcatatctt atctatttta ccacaaatcc gaccagcttg cacttgtcag
    12781 acaaatagga caaggagact actatttctt caaaaaatat gtcaaaaata cttgacactt
    12841 tatgtttaaa agactataat aaagtcaaat atatacgaca caaatcagaa aggagaccag
    12901 atgaatcgtg tgaaagaatt tcgcaaggaa ctgggcattt cccagctcga gctcgccaag
    12961 gatatcggtg tctcgagaca gactatcaat atgattgaaa acgacaagta caatccaacc
    13021 ttggaactct gtctcaatct cgcccgcagc ctccaaactg acctcaacag tctcttttgg
    13081 gaggatgatt tttaaaaaag gagcaaactc atgaaaaaag aaaccttcac tgaaaaactg
    13141 atcaaacgca catacggtat ttctggtccc cttgacgaat acaaacggcg tgaggccgat
    13201 cgtattggga accaagtctt tatcatcctc ttttatctga tgattttcgg aaatcttatt
    13261 ccactccttc tggcctataa ataccctcaa gaagtggctc taatctatcc tcctctgatt
    13321 ttagtgattg ccctcatcgc tgctggctat gtcacctacc aaatgaaaaa aacagggatt
    13381 acagctattg atccagacat gctgagcgag aaagaaagta agcaactaca ttacccaggt
    13441 ctgaaagcag gtttgttctt tggtctatgg atgtttttta taactcctct tctcaggata
    13501 ctcataggtg aaggtcagga ctattttcat tctcttctca ctataagaaa tggtgtatca
    13561 agcattctcg gttctatttt cttcggagcg agcatacagt tcctcatctc ccgtcgcatt
    13621 gcaaaaacta agaaaaatca agatgaggat taggaggtgc cctatgaaaa aagaaaagag
    13681 acagttacgt tatcctggtc tgaaagcagg ttcgatttat ggaacagtga tattttttat
    13741 aattccactc attgataccc taacaagtga aaatccaaat tttatcagtt ctcttctaaa
    13801 tacaaaacat atttttaaaa ctatactggg agctttcttt ttcggagtga tgatacatat
    13861 tgtcgactca cttcgtattg caagagctaa aaaagaccag gattaggagg taccttatga
    13921 aatcactagc tagactactg atcattcatg tttttatcag tattttcctt ttctttgccc
    13981 ttatttcagg agctgtttct catacagttt tactactcct actcctcttt cttcctgcgc
    14041 tcaataaagg acttgagaaa atacaatcaa aacggatacc tgtcctcaac gcagccctct
    14101 tctttctcct catatccttt ccacaacttt taaccaaccc tgtccaatgg aaattttcaa
    14161 tattcctagt cgtaaccatc atttcaagtt tggcctactt ctataacttt tatcaagtag
    14221 ttaaagaagt agatcaaaaa cagttgattt aggagcttgc tcccataatt tctcatttca
    14281 taggcaaata atcccaccta gcttgttcta ggtgggatta ttttatttct taacagagat
    14341 tgcaagtcat tttccttcat tttagaaagt cgactctttc caattcacac tacaatccaa
    14401 gacttgttgc ctttcttctt gattgcggcc ttcaatctgg tcaatcaaaa tctttgtagc
    14461 ctgttgtcct tcctcaaagg agggctgaac cagcgtcgaa acacttggag aagaaaagca
    14521 agtccactcc gtattgtcaa aaccaatcaa cccaacttgt ggcaagttat aattcaactc
    14581 tttgataacg gtaaagacta gaggtagggc ccaacagtta gggataaata ccagagtttt
    14641 ttcatcggga tcgatttctt tttgtaaaaa ttccttaatt tgttccaaat tcgtatgctt
    14701 atcttcaatg gttagactgg cgtgacgcat attagcatct gttaaagcat ccacaaaacc
    14761 acttgcccgc tcaatccgag tactcaaacg actcgtatcc gctgtaatca agagaaaatg
    14821 ttcataacct ttttcgatac aggactgggt catgtcataa acggcatcat agttattggt
    14881 tttaacccag ctagtccggt gttcatagag ctgactatca aaaaagacca ttttcttctt
    14941 tttctcatcg atgatacgag aatattttcg gaaattagag gtcggctgaa taataaagcc
    15001 gtctactccc aagagaagca tgctttcaat ataccggtcc tcactctctt ggctgtaatt
    15061 actatttcct atcattacct ggtagccatt ctggctggcg atatcctcaa ttcccttaac
    15121 aatttggttt gagaaactgt tggtaatatc accgatcaaa acaccgatta attttgttcg
    15181 tttggagttt aagctacgcg caacaatgct cggtttgtaa tttgtttcat gaataacttt
    15241 ttcaatcttt tcacgtgtct cttgggacat tttttcatat ttcccgttta ggtaaaatga
    15301 cacggttgtt ttcgaggtct gagccatttc cgcaatgtct tttatggtca gtttcttctc
    15361 caatctaaaa cacctcctac taatacttat tataacatgt tcactattca aaatgcctat
    15421 ttctagcact gcaagcgaat cattctctca tttattttat catatactag acattttcca
    15481 cgcatttttg ttccgtcaac gaggcacaat ttttcaccta caccaatatc ctcgcttaat
    15541 aacagaatca gcttgtctgt ttcgtcattt ttcacttcaa aagccagagc attttccact
    15601 tcacgatcat ctgtctggta gacagaatga cggatgattt caaaactctc atgcgcaatg
    15661 atggtataat ccagcatctg attctcaaag aattggcgct tggtgagttt gctacttctt
    15721 tcaagctcat tgtatttagg agaaatgatg gtatcttcca aatcaaaatc aacttcactc
    15781 catagtctca actgattgat tttcccatct tgataggtca catccttgtc aaggataaac
    15841 tgagtcaacg cctcatgctg accttgacac ctgatgtcat ccaccaagag ccagacatcc
    15901 tctaccaaca tgaggatttt tcttctgtga agataaggca aatcaggttc tgctgaccaa
    15961 taagcccctt caatataatg cattccctcc ctttctttat ggtgacaaaa cagggagtga
    16021 ggatagtatt catattccca ggatcccgtg attctttccg gagctttccc atctacaatg
    16081 caggtcgaat gactccaagc actctttaag agataacgtt catatatctc ccgataagaa
    16141 taacgcccag catctatgaa aataggttgg ccttgatact gtaagcaaaa actattctcg
    16201 tcactatggc tatgggcact tcctagcgga ccatttttga aaaatagata acgatgttca
    16261 tccttaatac agacatgtcc agagtcttca aagatcatgg acttaggctg ccaagctctc
    16321 ctttcaaatt cctgcagtcg cttgaccttt tctcgcccca ggaacaagag gctaagcaaa
    16381 tcaactttaa catccagacc gttaagaagg tcttccttgt tcaaaaccac agcagacagg
    16441 ctcaaaattt ctgtcgtttc tgtagaatcg ctatcaccaa aagccaaagt tcgtccatct
    16501 aagcctgtca tcatttgaat ataggtcgcc atcttttcca gcaactcttg gtaactatct
    16561 tgcaagtctg gaagcaagag acacaaatcc agcaaggctt tataaacctc tacatgatag
    16621 agaatcgact gttcaaactg gcttccatct cctaaaatct gtgtctcaat ttgctgtttc
    16681 aactcctctg aagcaaaatg gtaagcttct tctaggtcca tcttatctga aaagaaatga
    16741 tagatagcaa gcatcggaat tgtttgtaaa atcccccagt tactaagggt gtacttggcg
    16801 cgatagtagc ttttcataaa gtcaatctgc ttttctagac tgaccaaaat tttctctagt
    16861 tctttctcct ctagcaagtc aaatttcaag agaagcaaga gtagtttcaa ccaagtaaag
    16921 gaacgaatac ccgtatccaa tgttctagtc atcaaggatt gaggagaaaa ttctctcacc
    16981 tgctcaatcc aatcaaatag aaagaacttg cacttttgaa tatagtcctt atctccttct
    17041 accagatacc ctatcataaa ctgcaagaga tattcttgtc gattgagcat ataagaccat
    17101 tctggatcat cttcaaatac ttgatcccat accatcggct ggatttgatg gatttttgaa
    17161 caaggctcca tatcccaagg actatcaaac ataaaacgat tgtccatcaa gcgttcaagg
    17221 gaactcttga ctttctcata gtcttttgaa cagtgggaca agatataatc acgacattgg
    17281 tttccatcga ctctttcaaa aaattgtctt ctttcttctt tcattatcta ttaccagaaa
    17341 aagaactact taaaaagcag ttcttttgtc tttcccatta cactttcctt ttctacatag
    17401 atgaccacac cttttgcaat ctgcaaggag accaagtcat ctttgataga aatgattttt
    17461 ccatgaattc cagacaataa caacacttca tcaccagatg ttaaagaagc taaatactct
    17521 tgtcgttgct ccatctgttt gcgaagcaac ttttgctgac gaatagaatg aaagcttgac
    17581 agtaaaaggg gactcactgc caagacaatc actattccat aaaacaatgt tgtatccatt
    17641 aagctataat cttaagccag cttccgataa ttccgatgat aactgttaaa ataacgagtt
    17701 tatatgttgt ccatttcttt tctttgatca agtagtaaac taaaagtgta aatagggctg
    17761 gtagaagagc tggagcaacc ttatcaagca ttccctgaat acttacgata ctttgtttag
    17821 cgtctgcttt aacttcccct gcagcaaagg taatcggcac cataatctta acagatgtcg
    17881 ctgccaaacc agcaattacg gttacaccga taatattggc aatacgagaa atcgttgcca
    17941 tctgttcgct tagtttatca atcacagttg ttcctagttt gtatccatac agaccagttg
    18001 acaatttaat cgctgttaaa atcgtattca tcgcaaggaa gaacaagatt ggaccggcaa
    18061 ccaagccttc ttgagcaaac gaagctgcga tggttgagaa caatggagct aaacagaatt
    18121 gagaaagaga atccccaata cctgccaatg gtcccatcaa ggccatcttg atgctacgtg
    18181 tttcttttgc cggacggcca ttttccaaca ttacaagatg caagctggta ataaaaggca
    18241 ggaagtgtgg gttggtatta tagaattcac agttttcttc caaggcttgg tagaaaactt
    18301 cctgatcctc tccatagtgt tttttcaaag caggatacat cacattggca tatcccaacc
    18361 cttgatagtt actatagtta aatccatttt gacaaaagaa tgcccgcaaa gacgttttaa
    18421 gataatcacg ttttgttaat ttgttagatc cagtcatcgt gtgcttcctc ctctaccaca
    18481 tgatccgctg tttttggctt gttataaaat tcaatcaaag caaagatagt acctacaatt
    18541 gcaataccaa ttgttgggat gtttagataa gctgcacaaa catatcccaa caagacaaag
    18601 ggaatcaact ctttcttagc catcactgac aagatcatcg caaaaccgat agctgggagc
    18661 attttaccag caactgtcaa acctgtaagt aataccggtg gaatgtagtc tacgagtttc
    18721 aacaaggtat ccattgaaag ggcacccaag caacccaagg taaatccaat aaaggcaaac
    18781 aaccaaattg ttgcatttag agtgaacttg aatttcttca aattatggtt tttcaagtgc
    18841 ttcatcgccg tttcaggcgc accagcacgt acagtgtagg cgaaagtttg taagaattga
    18901 atcgccacag caatcggagt agagagggca agagccgctt ctggactgac tttaccagca
    18961 ctagtgatag ccatcaaggt accaaagata ccaggtccga ttgggtttgg tggaacagta
    19021 cctccagcac caacaccgaa tcccatataa gccaattcac caatagctcc cattgcaaga
    19081 gcagtaggca gatcacctag aataattccg acaccaaatg acagaacaat acatctattg
    19141 gtgtaaattc ctaacagcat tccactaaaa cagaaagctg tccataatcc aattaaaatc
    19201 gcttgaaata cattaatcga cataacgatc tcctctttta aatatagtcc ataatgttga
    19261 cttgaacagc tccatcattt cctgttggag ttgttttcgt attaaatgtt acttgatgag
    19321 tttggctcaa ttccttgagg gccaccttgt cctcttcacc caggaagatg gagcgtgtca
    19381 cttgctcttt accagtagca ttgtgaatgt tcccaatatt gatttctttg atagggacac
    19441 caccttctac caaggttaaa gcatccttca cattctttac aacgataaag atcgtttgag
    19501 caggattagc cttgtgaatg atatcaatca ccttttgcaa agggaagaaa cgcatggcaa
    19561 ctgagtctgg cacaactgtt ttcatcagag tttgttgcat cttgtccgtg cttacttcgt
    19621 cattggcaac aatgaccgta ttacaaccta ggtattttac ccaaagttgt ccttgtccat
    19681 gaatcaaccg ttcatcgata cgggtcataa taatatttgg cattgtcata tttctcctcc
    19741 taccaatata ggttccagtc tttgtagaaa cggataaggg cttctagata atagtagtca
    19801 ccccagatat tgccttcatc cactccttta cctgaatgcc atgagtacac accgtggagg
    19861 agacttgtcc caccaggggt aaattgatca tttgcataat gttcgatcaa ggaacgaagc
    19921 atggcatgca tagcatgttt ataaatatct ttgtcagcat ccacctctgg gagatgtttt
    19981 agcatttcat gaatcccaca gacggcgata gctgttgctg aagaatctcg tgattgatca
    20041 ctaccatcat taaaaatcaa atcccaatag gacacatgat cttttggcag acgattcaag
    20101 aaataattgg tcacaccctt aaacaagtca aagcaggact cgtcttttaa gtgacgataa
    20161 gtcaaaggaa taccatagac tccccatgat tgaccacgtg cccagcatga atcatcacta
    20221 tacccttgtc tcgttacacc tttaaagggt tgacctgtct caggatcaaa atagaaggtg
    20281 tggaaggacg aagcgtcatc acggattaca ttattagctg aagcatagaa atggctttct
    20341 gcaatatcgt agtatttttg atcgcctgtt tcttgataag caaagaataa gagttggata
    20401 ttgagcaagc agtcgataat caaacggtaa tgctctttct tgcccaagtc tccccaagct
    20461 tgaataaaac cacctttttc ttgatagcgt tcaatcaact tatctgcagc tttcaaggtt
    20521 gcttctctag cctctccatc tccatttatc ttatattcag ccatacaaga cggtgtgtac
    20581 aagaagccga gatcatggtg atccaattct actctcttat tgacacgatc caggaaagaa
    20641 agaacatttt tatgagcgat gtttttaaat gcatcctgtt gactgtattc ataagccaac
    20701 cacagttctc ctgtccagaa accattggtc cattccgtgt tatccatgat tggatagaca
    20761 ttatcaaagg tagctggagt cgggaaatct tccttgaaat agtcaaggtt gagttctaac
    20821 tgccgaataa ccttatcgat tgcctggccg acttcttctt tcgtcagaag tggtacttct
    20881 aagaagcgct caggcgattt tattttttca atcgtaacct tttttatcat tcttttaccc
    20941 tttaaatacc caattcgaaa tcttcttcct ctgcatccgt tgaaaacaat tctttaccat
    21001 ttacgactcc gccctgggcc gccactactg atttattaac aacctcatca aagctatgag
    21061 ccattctagc aaagactgct tccattaaca tggctaagtt caaacccgag agaacattca
    21121 ttgttttgac tggattttct cccattatgg tagaagaaac cttgaatggc gatcctccca
    21181 agagatcact taggattaaa acttcttctt catttgaaat tgcaagtaag attttttgct
    21241 tgagttcatc tgctgacatt ccttccacaa agtcaatcgc ctccacattt tcttgattac
    21301 ctgcaatcaa ttgtaaagaa ctataaatcc ctgtagcaaa atgtccatgc cctacaagta
    21361 caattttcat cttttctcct ttcttgttta ggtttaccgg tttactttat attccccatt
    21421 gtaatccttt atttttatct
//
LOCUS       DAVLYV010000028        21013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLYV010000028 DAVLYV010000000
VERSION     DAVLYV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.028"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(233..451)
                     /locus_tag="VYO70_001041"
     CDS             complement(233..451)
                     /locus_tag="VYO70_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713289.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(697..975)
                     /locus_tag="VYO70_001042"
     CDS             complement(697..975)
                     /locus_tag="VYO70_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713290.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAVYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1189..2820)
                     /gene="rlmD"
                     /locus_tag="VYO70_001043"
     CDS             complement(1189..2820)
                     /gene="rlmD"
                     /locus_tag="VYO70_001043"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1713291.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2894..3868)
                     /locus_tag="VYO70_001044"
     CDS             complement(2894..3868)
                     /locus_tag="VYO70_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1713292.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(3942..4556)
                     /gene="pmp23"
                     /locus_tag="VYO70_001045"
     CDS             complement(3942..4556)
                     /gene="pmp23"
                     /locus_tag="VYO70_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1713293.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4556..5530)
                     /locus_tag="VYO70_001046"
     CDS             complement(4556..5530)
                     /locus_tag="VYO70_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1713294.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(5538..6794)
                     /locus_tag="VYO70_001047"
     CDS             complement(5538..6794)
                     /locus_tag="VYO70_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1713295.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6856..7287)
                     /locus_tag="VYO70_001048"
     CDS             complement(6856..7287)
                     /locus_tag="VYO70_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713296.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7289..7891)
                     /locus_tag="VYO70_001049"
     CDS             complement(7289..7891)
                     /locus_tag="VYO70_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1713297.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7875..8714)
                     /gene="prmC"
                     /locus_tag="VYO70_001050"
     CDS             complement(7875..8714)
                     /gene="prmC"
                     /locus_tag="VYO70_001050"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1713298.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8714..9793)
                     /gene="prfA"
                     /locus_tag="VYO70_001051"
     CDS             complement(8714..9793)
                     /gene="prfA"
                     /locus_tag="VYO70_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1713299.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9790..10380)
                     /locus_tag="VYO70_001052"
     CDS             complement(9790..10380)
                     /locus_tag="VYO70_001052"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1713300.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10503..10685
                     /locus_tag="VYO70_001053"
     CDS             10503..10685
                     /locus_tag="VYO70_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1713301.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10822..12195
                     /gene="mnmE"
                     /locus_tag="VYO70_001054"
     CDS             10822..12195
                     /gene="mnmE"
                     /locus_tag="VYO70_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1713302.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQIEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12242..13177)
                     /gene="dapA"
                     /locus_tag="VYO70_001055"
     CDS             complement(12242..13177)
                     /gene="dapA"
                     /locus_tag="VYO70_001055"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1713303.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13233..14309)
                     /locus_tag="VYO70_001056"
     CDS             complement(13233..14309)
                     /locus_tag="VYO70_001056"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1713304.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEKSTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGGSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14652..14724)
                     /locus_tag="VYO70_001057"
     tRNA            complement(14652..14724)
                     /locus_tag="VYO70_001057"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14689..14691),aa:Thr,seq:ggt)
     gene            complement(14824..15333)
                     /locus_tag="VYO70_001058"
     CDS             complement(14824..15333)
                     /locus_tag="VYO70_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1713305.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15331..15427)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15456..15887
                     /locus_tag="VYO70_001059"
     CDS             15456..15887
                     /locus_tag="VYO70_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1713306.1"
                     /translation="MKIPIQKFFNNEILAYLFFGIVTTLVAILLRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            15969..16346
                     /gene="mscL"
                     /locus_tag="VYO70_001060"
     CDS             15969..16346
                     /gene="mscL"
                     /locus_tag="VYO70_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1713307.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16388..17482)
                     /gene="hemH"
                     /locus_tag="VYO70_001061"
     CDS             complement(16388..17482)
                     /gene="hemH"
                     /locus_tag="VYO70_001061"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1713308.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSKQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            17637..18860
                     /gene="pepT"
                     /locus_tag="VYO70_001062"
     CDS             17637..18860
                     /gene="pepT"
                     /locus_tag="VYO70_001062"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1713309.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIESIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(18896..19525)
                     /locus_tag="VYO70_001063"
     CDS             complement(18896..19525)
                     /locus_tag="VYO70_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713310.1"
                     /translation="MSKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLEPTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            19490..19711
                     /locus_tag="VYO70_001064"
     CDS             19490..19711
                     /locus_tag="VYO70_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713311.1"
                     /translation="MDLFQELICLAQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(19848..20057)
                     /locus_tag="VYO70_001065"
                     /pseudo
     CDS             complement(19848..20057)
                     /locus_tag="VYO70_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad protein"
     gene            complement(20144..20407)
                     /locus_tag="VYO70_001066"
     CDS             complement(20144..20407)
                     /locus_tag="VYO70_001066"
                     /inference="COORDINATES: protein motif:HMM:NF016182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1713312.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAT
                     YVFNPNDIVRDTGDAYIVRHGVTITIFLSHLLTIPHHTLTRKR"
     gene            complement(20451..21011)
                     /locus_tag="VYO70_001067"
                     /pseudo
     CDS             complement(20451..21011)
                     /locus_tag="VYO70_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         6056 a         4373 c         3921 g         6663 t
ORIGIN      
        1 atttgtttgc taatatctct ttcttctgat tttgtttcaa ggattattgc tacatcattc
       61 atatttttag gtaaatacca accatctggc ttatctgaat atcccttgaa gcctaattga
      121 ttaaaagtag ttatttgacc agtaccttga ttgatgtttt cttcaacttc attaaagcct
      181 atacttctct tgcataatcc ctaacttgat cttctgtcct taaagtcatt ttctattcct
      241 cgtcactaaa attatcttca aaactaataa tatcattagg agtgcaagat aaagccttac
      301 atattctttc taaattctta aatgtaattg gtttatcctt tcccatttgt gccataacat
      361 ttgttgtaag tcctgccata gctattacat ctgttttctt tagtcctttt tttgctaact
      421 gtatccataa tggtttatag ttaagtgcca taatatccct ctttttttct tcattagatt
      481 tatttaatta tagcataaat attgatttca ttcaatctta cattgattta gtgatttgtt
      541 ttagagaatt tgttgtgtta ttccgtcctt attttgccat aaattgtttt aagtgggacc
      601 tcttatcctt accattttta cctcctaatt ttgagtataa aaaagacgat agagttttta
      661 atttctatcg tctgagattt acatattaaa ttttcatcac aaaataagac tctttaaaga
      721 atctttaaat tcattggtat gctgcttaca atatccatta ttaaaatgga tactataaac
      781 agcacaccaa ctatttattt tttgagtttt aaactttggt agaagtcttg aactccttat
      841 atttacataa ctatttgcct ctataactat tatgacacga aaccaacaag caagttcaag
      901 cacctccata tctttattca aatgaggact aagatattca caaactttat cataaacctc
      961 tttatctttt ttcataaaag gggcatacaa cttagcaaat ttatcctaaa attttttgtt
     1021 gcctttatca atcatttcaa cactccttta aatataaaaa tgatcctatc aatctttata
     1081 ttgagcctat tttctaattg ataccttgca agaaaggaat aatagccgtt cctataccaa
     1141 gtaggaatgt aataaatgca gaaagtcata tactgtcatt cttttctatt aaatcatttt
     1201 gaagtgtctc aaagcatcca tgatggcttc ttctttttca ggtgtacact gtggaataat
     1261 ttgtttatcc tttttagact tgttgtaatg ttctcgtaat tctattccac atttcttttt
     1321 tatctgtgca atatataatg tcgaaacttt taattcaaat ttattccaaa catattcttt
     1381 gatttgagca tatgttgctt tgctctccgc acttgtcaaa ttcatctcat ccagctcaat
     1441 ttcaacactt atgtgcttat cgacatcgag tttggacaaa agtgctaccg tctcgacgtg
     1501 atgcgtttga ggaaatagat ccaccggctg gactttcttc aattcatatc ccaattcttg
     1561 gtagagtttg atatcacgcg ccatggttgc gacattgcag gagatatagg cgatgcgatc
     1621 ggctcctgtt tgggcgcttg ctttgataaa gctttctgtc aagcccttgc gtggaggatc
     1681 aaccaagata acggttggtt gaatcccatc tttaagccaa ttcttcatag cattttcagc
     1741 tgtgtcacag acatagtggg cgtttgaaat attgttcaac tgagcatttt ttttactatt
     1801 ctcaaccgct tctggaatca gttcaacacc gtagacttct ttgacatgct tggcgactga
     1861 taaaccaatg gttccaatac cagaataagc atcaataacc acgtcatctt tttttaactc
     1921 tgcaaagtca atggctgttt gatagagttt ctccgccatt tcagtattga cttggtaaaa
     1981 ggctgggcca gcgatttgga agtcatttcc caacatctgg tccgtaatat agtcttgacc
     2041 ataaagagtg cgccactcct taccaaaaat cgcattggta ttctggtcgt tgatattttg
     2101 catgacagac acaatctctg ggaactgctt gataacttgt tcaatcaatt ggtcaacacg
     2161 aaaaactttt ggacgagttg tcaccaaaac gaccatgatt tgtcctgaat agtgaccacg
     2221 acgcaccaca agattccgaa tcaatccaga ctgttccttt tcgtcataag gttttaaatc
     2281 aaaacgacgg agcaggtctc gaagagctac tacgacttgg tcaatgacag gatcctggat
     2341 aaagaaatct tcaaggggca tgaggttgtg cgaattctta cggaaaaatc ctgtttccaa
     2401 gacaccattc actcgacgaa cgggcacctg cgccttattg cgatacttga ctggatgttc
     2461 cataccaagc gtttcagcaa cttctacatc tgcaattcca gcaatcttgt agagactgtc
     2521 cttgacttgc ttggttttaa acttgagctg ttctggataa gaaaggtgtc ctaaatccgc
     2581 gattcctgaa cgcaggtaag ccaaatctag atcttgatta cggtgtggtg actggacaag
     2641 gtatttttca acttttccaa agccaatctt tttattgacc ttgaggacac gcatgagaat
     2701 tttttcactc ggtaaagcat tctctacaaa aaagaccaaa ccatctacct tggcaactcc
     2761 tgccccttca tgggtcaaat caacaatttc aacttctaca atatcatttt tctttaacat
     2821 tcttttctct ttctattggc cgacaaaaaa gacggcaacg ttacggttac catccattat
     2881 accatgaaat ttcttaagaa ccaaaactct tgaagaagtt gacaagggct tgccagaggg
     2941 agcttaagaa gttaccaccg tcctctagcg ccttatccgc atcaaagtta aggttgatat
     3001 ttttaaaact gtcgccagct tgtgatacga tgctttgttt aaggtcattt agggttttag
     3061 tgaaatctgc attgctgagg atatcactct ttgagagatt caaggcaaaa ttgatgatga
     3121 tattgatctg gtttcctgtt atgacctgat caagtttgta attttttaag gtatcttcaa
     3181 caatcttgcg gatatcttct tctgtcagat ttcccttgct ttctttagct ttggcgagtc
     3241 ctgacttgat atcagctagg gcaacgttta atttattagc atcatagcct gatttgtcct
     3301 tgttttcagc attgatatct gacaaagcct ttagctcttc ttgagccaaa tctttattgg
     3361 cttgcggcac cttggctcca ttagcttcta gcgaatagta gatcccagcc aaagcactct
     3421 cacctgtaac tggaatgggt gctgctacag tgattttggc gtgttccaca cccaaagtta
     3481 cggctgcatt tcggtacata tcctgagtta ccttagtgat gttttcaggt gtttcaatct
     3541 tgacctcaag tggcgatttg tcacctagct tttgaatctt ggctgatgaa tacaactgta
     3601 agctagagtc attggccaca ttcatgattt tagaataaac atcaggtgtc atggtcttga
     3661 gttctttggt atctgttgag gcattgtagc ccagtttttt aagagtttga tttttttggt
     3721 cttcagatag ggaagaacct aggacatatt caggttggac ataggtttca tcgataactt
     3781 tttgaacatc tgttgctgca tggacgctat tcatagctgt tactgcccac aagatcgcag
     3841 cgctagtcag aaagagtttc tttctcatag ggaatttcct cctttacttc tttagagtaa
     3901 tatatctatc ttaaagaaaa cttataacaa aaacacctgg tctagccaga tgttgaaaag
     3961 agagtgaaac atttgataat gtaaaggtta agtcgtacct gtctagaata ataatagttt
     4021 cctccattta catagagttc agcaccgtga aaaatggaaa tggggtgaat ataactataa
     4081 gtctttccag tcgtattacc aagcaagggg gcaacagtct cacgagagta ctgtttggct
     4141 agagccaagg tattttcctt gccattttgg gcgataaaat cgatataggc aggtccaaaa
     4201 ttataggctt gaacagctgt ccagatatct acacccttct tctgcgccag atagagattg
     4261 cctgtcagag tttgaatgcc ttgccgaatg ctagaggcat tatcattgat ggtgttggtg
     4321 gaaccacttg cagactcact agactgcata acatcgcctt cttttccttt tgtttcagta
     4381 taaatcatag caagcacaag ctcttcgttt gctggggtgt cttgttcact caatatttct
     4441 cgcaccatgg gttgataggt catgacttgt ttgacatctt gatgaacgcg gtaagcttta
     4501 tagccagcaa aaaggaagac tgctagtaca agcactcttc gaattcgttt aaacattatt
     4561 tactttggat atcctcgata tttttgatta agatagagta ggttccattt tcgttttgga
     4621 taaactcaac agactcggcg tcttgataga cgttattggg aacgatgagc tcaattccat
     4681 ttgataagga gagtttttgg ttttcaaatt tctttaattg gcgactggca tcaatttcat
     4741 caaattgaac aggttctggt acggcttctt tgacttggtc aataaagctc aaacgagccg
     4801 tcagattgtt gtcaaaaagg tcattagcca atttctcagg tgacaattca ttgctttctt
     4861 ctaggttgtt gaaaatagct gatttgacct tggattgaaa ttgaaaatca tctgtgttaa
     4921 aagattcagc aattctctgg gctgtttttt ccagttcctt gatagatttt ttaggagaaa
     4981 tcttaggagc gacagcaaga agattatctg aaaaatagtt caaaaaagtc ccgttgtact
     5041 tgattcgttt ttcaatcagg tgatacttgc gactctgaag attgaccacc aaggcctcgt
     5101 cagcacccgt tccaaatcca ggcaggttat tctgagtcag cttgattgga ttatcaactt
     5161 ctcctccgag gtgggtcaag gtctcccgca gggcaattcg caagaaagcg aaatgttcta
     5221 caccttcttt agaaaattga acaaaaatca agtcattggt cttgagattt tcagaaatgc
     5281 taaactcctc tttccagaga ttagccagcg ttactgatgt ctccaacaaa tcgtctgtaa
     5341 tatgattgaa gaagggattt tcttcttcga aaatcccagt cttggcttca tctgaataca
     5401 catgttcaat ttttttacgt aggtattctt cgatttttgg agtaatattg agaaacttat
     5461 ctgctaagaa cagctcggta tcatccggac tgaactggtg aataatggct ttcttaatat
     5521 aaatgtccat aaaagtttta ttcctcgtat aatgggaagg catctgtcaa ttctttgact
     5581 gcacttctca cttcttctaa tacagcctca ttttctgaat tcttaagggt tttaatgatg
     5641 agttcagcca ctttgcgact ttcttcttca ccaaatccac gtgcagtaat agctgctgct
     5701 ccgatacgaa tcccacttgt cttgaatggt gacaaggttt cgtaagggat tgaattttta
     5761 tttagggtaa tattgacttc atccagcaag ttttgagcaa cttttccgtt ttctacaacc
     5821 ttagtcacat caacaaggaa gagatggttt tcagttccac ctgaaataat acggaaatca
     5881 gggtcttgca agaagacatc tgccatagcc ttgctgttct taattacatt ggcagcatat
     5941 tccttgaagg ctggatccaa aacttctttg aaggaaactg ccttagccgc cacaacatgc
     6001 tctaaaggac cgccctgaat acctgggaaa atagctgaat tgattttttt agcaagttct
     6061 tcgtcattgg tcaaaatcaa accaccacga ggtccacgaa gggttttgtg ggtcgttgtt
     6121 gtagtgatat gagcgtatgg cactgggctt gggtgaagac cagctgcaac caagccagcg
     6181 atgtgggcca tatcaaccat aagcttcgca ccgacagcat ctgcgatttc acggaatttt
     6241 gaaaagtcaa taatttgaga ataggctgaa gcaccagcta caatcagttt tggttttact
     6301 tcttgggctt gtttcaagat agcatcaaag tctaagagtt ccgtttcagg atcaacacta
     6361 taagaaacaa agttgtaggt ttgaccagag aagctaacag gagccccatg agtcaaatga
     6421 ccacctgatg ccaaatccat tcccataacc gtatcacctg gctcaatcaa ggacatgtaa
     6481 gccgcacagt tagcttggct tcctgaatgt ggttggacat tggcaaattt agcaccgaaa
     6541 atttcttttg cgcgttcaat agcaagagtc tctacaacgt ctactacatc agttccacca
     6601 taataacggc gtcctgggta accctcggca tatttatttg tcaagataga cccttgagct
     6661 gccataacag ccttggaaac tacgttttcc gaagcaatta actcgatatt attttgttgg
     6721 cgttcttctt ctttggcaat agcattccag agatcagcat catatgcttt aaaatcatct
     6781 ttgtcaaaaa tcataggtct tctcctttat tgtgtgacta gtccattagt ttgattttac
     6841 aataagaaaa tcaaactaaa agatgcgaat aaaccgtttc tgcattttat cacaagtata
     6901 gccaactttt tcataaaatg catgagcacc cagacgatga ttggcagaat ttaagcggat
     6961 aaacccataa ccacatcttt tggcttcttg ttccaaccct tgtagtaaac ttttaccgat
     7021 accttgacct tgcgcttgag gtgaaactgc taaagctaag atattaaatc ctgctttgga
     7081 atagagtgat tcgtaaactt cagcgtggac atatccaagt aagacatgat tagctgcatc
     7141 ctcatagcca agtaggaaat gatgggaatc ctgagacagt ctagctagtt ggctagccgt
     7201 ttcctctgga ctaaaagtat aacccaaagc ctcttggttg atgtcacata tcgctttcac
     7261 atctgtttct tgcaaatctc ttagcatttc aagcctcctc aaaagaaatc tctggcaacc
     7321 gagcaagaat atcttctcgt ttaattgcgc cttggcgtaa gattttcacc ttgtctccag
     7381 acaaatccac aatagttgaa tcctgtccag ttagaaaagc atcgtcttcc agacccagaa
     7441 cctcttggtc aaaatccttc agaatttgtt caaaggttac accacttgcc tgacctgaga
     7501 tattggcaga cggcccaatc aagggacctg tctctcgaat taaatccagt gtgatagggt
     7561 gactgggcat ccgaaatcca atagttgcaa ggtcagaatt tacccaatag ggaactcggt
     7621 cattggcttc gagaataatg gtcaagggac ctggcaaaaa ggtctctaca agtttttgta
     7681 gataagttgg ctgattctta gaaaagtgca agatatcctc gaaagaggcg atattgagat
     7741 tgagtgcctt atctctagga cgacgtttga gttggtaaac atggtcaact gctttttcat
     7801 ctaaggcctt ggcaaaaaga ccataaacag tctctgtagg tagaacgaca gctccaccat
     7861 tttccaactc ttgtctaatc ctgtccatca tcaaccacaa ccatcctatt ttgaccaaat
     7921 tggtctttga gtgttcgaac ttttttttca ggaagatgtt ttctaaaaag ttcgggaaca
     7981 ctttgacctt gcttgtatcc aatttcaagg taaatcttac caccatcctt gagatagtct
     8041 tttgcatctt ccgcaattct acagtaaata gctaggccat cctcgtctgc aaagagagct
     8101 agatgaggct ccgaatgcaa aacattcaag cctacctctg actcatcttc acgagagata
     8161 tagggtggat tggatacaat tatatcatat ttttcagaaa tttctgcaaa acaatcagat
     8221 tttttaaaaa atatattaag attttgatcg ctagcattct ctgatgcaag ctctaaggcc
     8281 tcttgagaaa catctgctgc tgtcactgac caatctggtc tattttttgc tagagcgaga
     8341 gcaatagctc cactacctgt tccaatatcc agaactgaga gattcgtctc aagattttca
     8401 gttagaataa gctccactaa ctcctctgtt tctggacgag gaatcaaaac ccgttcatcc
     8461 acctttaaat gcattccata aaaatctgcc tgtccaatga tgtactgagc tggcttgtga
     8521 gctgctagtt gctggtaaat atcttctaca aattgttttt cttcctctgt tgtcacctcc
     8581 tgctggaggg caaaaataaa gtctgtaaaa gatagatttt tcagactacg atagacaaaa
     8641 gagaggcttt ccgcttcctc tccttgtctt atcaactctt cttcaaaatt tgaaaataat
     8701 tgagctaatt tcattatttg tttaattctt ctagtttttg tgtttggtca taaagcacca
     8761 aggcatccac aacttcgtcc aatttaccag acaaaatcgt atctagtttt tggagggtca
     8821 agccgatacg gtggtctgtg acacggtttt gagggaagtt ataagttcgg atccgttctg
     8881 aacggtcacc agtaccgatt gtcgacttac gctcagcgtc ttgttcatcc tgagcaatct
     8941 gagcaaagtg gtcagcgacg cgtgcacgga taatcttcat agccttctca cggttcttct
     9001 gctgggtacg ttcttcctgc atctcaacct tgatattggt tggcaagtga acgatacgaa
     9061 cggcagtcgc aaccttattg acgttctgtc caccagcacc tgatgcgtgg taaatatcaa
     9121 cacgaagatc ttttggatcg atatcgtatt ctacttcttc gacttcaggc atgacaagaa
     9181 ctgtggcagt tgaggtatga acacgacctt ggctttctgt cacggggaca cgctgcacac
     9241 ggtgggcacc tgattcgtac ttaagcttag agtatacaga ctgacctgaa accatagcaa
     9301 ccacttcttt aaacccaccg acaccattca tagaggcttc catgacttca aagcgccagc
     9361 cttgggcttc cgcatacttt tggtacatag ttagcaaatc tccagcgaaa agtgccgctt
     9421 cgtctccacc agctgctcca cggatttcaa ggatgatatt cttgtcatcg tttggatcct
     9481 ttggaaggag caaaattttc agtttttctt catattcttc tttttcagcc ttggcatctt
     9541 tgagttcttg cttggccatt tcttccaagt ccgcatctcc gcctgattcc ttaatcatct
     9601 cttcggcatc gacgatattt tgaaggactt gtttatactc acggtaggct attacggtat
     9661 cacgattgga agcttcttct tttgaaagct ccataaaacg cttggtgtct gaaacgacat
     9721 cagggtcact cagcaattct cctagttctt cataacggtc ttctacagct tgtagttgat
     9781 catagatgtt catttttctt cattctcctt attgatttca ggggcaaaat aatgtttacg
     9841 gcaaaccgag atataggttt cattaccacc aatttggatc tgttctccat cgtaaacggg
     9901 cagtccatcc tgtgttcgca acaccatggt cgcctttttc ttgcaatact gacagatggt
     9961 cttgatttcg tcaatcttgt ctgctaaaag caagagatat ttggaacctt cgaacaattc
    10021 attgcgaaag tcatttttca agccaaaagc catgacgggt atgtctaact cgtccacaac
    10081 acgagctagg tcataaacat ggtggcgttt gagaaactgg gcttcatcga ccaacacaca
    10141 gtaaggtttt tctggtaggc ctcggatata gccaaagata tccgttgttt cctcaatcgc
    10201 aagggcaggg cgtttcatgc caattcgact cgacacatag ccaacaccgt cacgcgtatc
    10261 cagagctgaa gtcataatca caacaccttt tccttgctcc tcatagttat aggccacctt
    10321 gagaatctcg attgtctttc cagagttcat ggtcccataa cgataataca actgtgccat
    10381 gtttcttgct tcacgtccat ttctaaattt ttgctacatt ctagtatatc ataattttct
    10441 taagctttaa acggcaaaat gtggtaaaat ggaagaaatc aaaactagtg gaggaagcta
    10501 ttatgccatt tgtacgcatc gatttatttg aaggacgcac gctcgagcaa aagaaagctc
    10561 ttgctaagga agtaacggaa gcagttgtcc gcaacactgg agcccctcaa tctgctgtcc
    10621 atgtcatcat caacgacatg ccagaaggaa cttacttccc acaaggggaa atgcgtacta
    10681 aataagctag cttaagcaga attgcttagg ctttttcaat ctccaagtag cattcattga
    10741 agaaatatcc taaatttgtt acaatttgaa aagaaacttg gagaatttcc aagaaaatag
    10801 ctattaatta aaggaaacat tatgattaca cgtgaatttg ataccatcgc tgctatctct
    10861 actccactag gtgaaggggc tattggtatt gtccgcctga gcggaacaga cagttttgct
    10921 attgcgcaaa agatttttaa aggaaaagac ttgaacaagg ttgccagcca cactctcaac
    10981 tacggtcaca ttattgatcc tctgactggt aaagtcatgg acgaggttat ggttggggct
    11041 atgaagtctc caaagacctt cactcgtgag gatattatcg agattaacac ccacggtggt
    11101 attgcggtga ccaatgagat tctccagcta gctatccgtg aaggagctcg gttggcagaa
    11161 cctggtgaat ttaccaaacg tgccttttta aacggtcgcg tagatttgac acaggccgag
    11221 gcggtgatgg atatcatccg cgccaagaca gacaaggcca tgaacatcgc ggtcaaacaa
    11281 cttgatggtt ccctttctga tcttatcaat aatatccgtc aagaaatcct taacacactt
    11341 gcccaaattg aggtcaatat cgactatcct gagtatgacg atgttgagga agccactact
    11401 gctgttgtcc gagagaagac aatggagttt gagcaattac taaccaagct ccttaggaca
    11461 gcacgtcgtg gtaaaatcct tcgtgaagga atttcaacgg ctatcattgg acgtcccaac
    11521 gttgggaaat caagccttct caacaacctc ttgcgtgagg acaaggctat cgtaaccgat
    11581 atcgctggga caacacgaga tgtcatcgaa gagtacgtca acatcaatgg tgtacctctc
    11641 aaattgattg atacagctgg tattcgtgaa acggatgata tagtggaaca aatcggtgtt
    11701 gagcgttcga aaaaagccct caaggaagcc aacttggttc tactagtgct aaatgccagt
    11761 gaaccactga ctgcgcaaga cagacaactt cttgaaatta gccaagatac caatcgcatt
    11821 attctactta ataaaaccga cctgccagaa acgattgaaa cttcgaaact acctgaagac
    11881 gttatccgta tttcagtcct taaaaaccaa aacatcgaca agattgaaga gcgaatcaac
    11941 aacctcttct ttgaaaatgc tggcttgatt gagcaagatg ctacttactt gtcaaacgcc
    12001 cgtcacattt ccctgattga aaaagcagtt gaaagcctac aagccgttaa tcaaggtctt
    12061 gagctgggga tgccagttga tttgcttcaa gttgacttga ctcgtacttg ggaaatcctc
    12121 ggagaaatca ctggggatgc tgctccagat gaactcatca cccaactctt tagccaattc
    12181 tgtttaggaa aataagaaaa atccatgatc cttcattcgg tcatggattt tattgtcttt
    12241 attagtaatc tggtcttaag acccctgtta cagttgcctt agttgcttcg tagtcgccat
    12301 ctacgacaac cttgataatg cgtttggcat cttcttctgg tgctggaaca agaggtagac
    12361 gagtgggtcc agcttcaaat cccatatagt taagaattgc cttaactgga gcaggacttg
    12421 gataagagaa gagagcatta accttaggaa tgaatttacg ctgaattgct gcggctttct
    12481 tcatatcgct ttctgcaatg gcagtaaaca tctcgtgcat ttcatcccca tttgtatgag
    12541 aggcaacaga aataacccca tccgccccaa ggttcatggc atggaaagca tctccatcct
    12601 cacctgtata aatcaagaac tcttcaggct tgtgctcaat caagtaagcc atattagcca
    12661 agctagtaca ttctttgaca ccgataatat ttggatggtc agccaagcga agcatggttt
    12721 ctggagtcaa ttcgacaact acacgccctg gaatgttata gataataatt ggtaggtcag
    12781 aagcatctgc aatagtctta aagtgctgat acatcccttc ttgagaaggt ttgttgtagt
    12841 aaggaacaat agcaagccca gctgcgaaac caccaaattc cgctacttct ttgacaaact
    12901 caatagagtc acgcgtatca ttagtaccta cacccgcaat caaaggaacg cgtccattga
    12961 caaccttttg tacagccgca aacaactcca actcctcatc gtgggtcaaa gttggactct
    13021 cagcagtcgt tcctgcgaga agaattccat ccgtatgatg ggccaataaa tgctcaatca
    13081 aggctggaat agcatcaaag ttaatagaac catcctcatg aaagggggta ataaaggctg
    13141 taatgatttt acattctttt aaatcttgat aagacatgaa aacacctctc tatttcaaag
    13201 aaggagttca tctgaactcc taaacgatat gactatttta attcaaattt caattcggct
    13261 gttggacgaa ccaatccacg ttcatgaaga gtttcagcaa tctgaactga gttccaagca
    13321 gcacctttga gaaggttatc tgaaacaacc cacatgtgaa ttcctttttc tgcatccaag
    13381 tctttacgga tacgaccaac aaaggtatca cgcgaaccaa ctgcattgat agcttgagga
    13441 tagatttgat gagctacatc atcttcaaga acagcacctg ggaaggctgc gatagctgct
    13501 tttacttctt cgattggagc cacttctttt gtttcgatat aaacagactc agagtgagct
    13561 gacaagactg gaatacgcac acatgttgca gatactgcaa tgctatcatc ttccataatt
    13621 ttcttagttt ccttggtcat cttcatctct tcgtaagtgt aatcattgtc agtgaagaca
    13681 tcgatttgtg gaagagcatt gaaggcgata ggataatgtt tcttgtcacc gcctgaaggt
    13741 aagatttccg catgcaaatc acgtggtttc actccatcat tcaagacctc acgaagttca
    13801 cgttgtgtct caagaattgc tcccatacca gcacctgaaa cggcttgata agttgaaaca
    13861 atgatacggt ccaagcccca tttttggcga accggctcaa gagccaccat catttggatt
    13921 gttgaacagt tagggcaggc aatgattccg ttgtgggcat caagtgcatg agcattgacc
    13981 tctggaacaa ccaaaggaac atctggattt tgacggaaat aagatgtatt atccactact
    14041 accacgccag cttttactgc gtatggtgca tacttagctg atgtcgaacc acctgctgaa
    14101 aagagagcaa tatcaactcc ttcaaaagct gtttcagtcg tttcttcaat cgtaatatct
    14161 tgatctttaa atttcaatga cttacctgct gaacgtgcag aagcaaggta acggatttta
    14221 tcgattggaa gtgttgattt ttccaacatt tttatcatct gagcaccgac agcacctgtc
    14281 gcgccgacta cagcaactgt atatcccata aataacctct ttcggaattt tctaaaaatt
    14341 tctataatag aagtattata ctactttttc catgaattgc aaagcttttt catcattttt
    14401 tagaaaataa aaatccccaa tcgctagact agggattaag aggcatcttc atgtgatgag
    14461 caggttcaca caactcatca aggtccgctc ctgcgttatg acctccttat gctcaatagt
    14521 agcccgaaga ctacctatcg actcatcgca tattctatat tactagagat agttgatttt
    14581 gtcaataaga aattttttcg atcgagtata acaaaaaact ctcgtattca agagtttccc
    14641 tatacatatc atgctaattg ccgggattga accggcgacc tcatccttac catggatgcg
    14701 ctctgccaac tgagctaaat cagcttactt aagaagtata ctatattcag aaaaacttgt
    14761 caagtttcct tagaaatttt ccataggaaa aagccaggta agaactacct agcttatctt
    14821 cttctattta cttaaatcaa ttcgacgacc aattttacca ataataatcg ccccaataat
    14881 caatgaagca aattcaccga ttcctgttgt aaaccaagta aggaagaatg gtaattctgc
    14941 tacaatattt aactctgcag cgatagtaaa catggaaatt gaaaagagga ttgaaaagaa
    15001 gaaatgatct tttcgaatca atccgttaaa gaggtaatcc ttgctatatt ttgcaaaaag
    15061 ccaaacacct aaactgagga atactaaggt tgaaccacca ccaacaaaga catctagcag
    15121 tccgaagcta aagaaattag caatcataca accgatggta actccgatga tgtacttagg
    15181 attgtaaaaa gccataaagt tcatcatttc tgaaatacga aactgataag caccatagct
    15241 gatggcattt aggggcggtg tgacagtcaa aacgacgtag atagcagcga cgattgcaat
    15301 atctgcaaca tcacgaatag ttaatttttt catcttttct cctttggcgg ttgcccgcgt
    15361 gtaatatgct tggtgaaaga agctaagcac caagggttgc tttattcaac cctactagta
    15421 tagcacaata gcctgcttta tgctatactt aacacatgaa aattcctatt caaaaatttt
    15481 ttaacaatga aatcttagcc tatctctttt ttggtatagt gactactctt gttgctatac
    15541 tattacgatt agtcatttat cagctaagtc atcaagaact ccttgctacg gccttggcaa
    15601 atattatcgg tattttattt gcctttatca caaatgatac aattgtattt aagcaaaaaa
    15661 agaaaaatag acttatccgt ttagtgaaat tttctcttgc tcgcctttct acttttctgt
    15721 tagacttgtt tttaactttt ctctttgtaa cccaatttcc tcacatcata gggcaattcg
    15781 taactgaaaa cattgataag ataaatgcta ttgaaacaat aattgcacaa atattcatta
    15841 ttatcatcaa ttatatcttg agcaaggtct atatttttag aaaataatgc tccatttatg
    15901 agccttttaa ttgccttcta taatgattta ggatacaatg aaacacgaaa cattgaaagg
    15961 aaaacagtat gttaaaaaat ctaaaatcgt tcttgcttcg aggaaatgtt attgaccttg
    16021 ctgtcggtgt tgtaattgcc tctgcttttg gtgctatcgt tacttcactt gtaaacgaca
    16081 ttatcactcc tcttatttta aatcccgctt tgaaagctgc taaagttgaa cgtatcgctc
    16141 aactttcttg gcatggagtc ggctatggta acttcttaag tgctattatc aattttatct
    16201 ttgtgggtac cgccctcttc tttattatca agggcattga aaaagcacag aaactgactg
    16261 gcataaagga agaaaaaact gccgaaaaaa aaccaaccga attggaagtc cttcaagaaa
    16321 taaaagctct ccttgagaaa aaataatcga ttaaaaggat ctagcgcaaa gctaaatcct
    16381 tttttcttta ctctttgggt aacttgcctg ctttttcaag catttttttg ataagataag
    16441 gcatcttaac attctcccgc ccttttttct caatcagttt tttcacaaat tctggcattt
    16501 gtaaatcttc agattcagcc aaaatgtttt ttgtctcgtc tgaaggaact aactcatcaa
    16561 aggtctcttc ttctgggaga aattccttaa actcttgatt ttcattgact ctaactgtat
    16621 tgaccaaagc atcaatcaaa cgagaatctg tatttggcat tggtggacga tggtagttta
    16681 ccccaaattc ctgacataaa tcataacatt ccacatcatt gtcaaacaag acctcaatat
    16741 gctcactgat gaaactaata ggtacaaaaa tataatggtc tggatgttct gtctgttctc
    16801 tgagatactc caaaacatct ggtttaatcc atggaatacc aatatcactc tcactctgcc
    16861 aagtgttagt atattgtttt gaactcaagc ccagtttttc agcgactagc ttgctatttt
    16921 cgaaaatttg gtctatataa ggatcaccaa agtccaaggc aaaaataggc acactatggg
    16981 ctgaaaagat gactttaaag ctgtcttgct ttacttcttc ttttaaaatc ttagcaattt
    17041 catctgccca atagtttaag agcgcttctt cctgatacca gtccttaatg accaaaaatt
    17101 gaatttgttt gctttctaaa aatttttcat agcccatgac agagtagaaa gaataatggg
    17161 gctccaaaat caagcaaata cactgttcaa ttccgtctgc ttccatttga ccgatcacat
    17221 ctggaataaa gggactggaa aacttattag caaagtagac actgtattca ttccccagcc
    17281 tagcttctac caaggcaacc tcttgacggg taattttttg cagaggcgtg cctccaattc
    17341 gtacataatt atcatagaga gtctgaatct cgtggtcttg gggtctcacc ccacgacgaa
    17401 tatttgtgaa aaaatcagct acaccttcaa aggtaatctc ttctggtgaa ccgaaggtca
    17461 tcattaaaat tgcttttttc atgactgctt ccttgtgatg tttttatcaa gtttatttta
    17521 tcattaattc atcgataagt aaacgctagc atagggacat tctcaatctt ctgtcttttg
    17581 tttttctctt ctttctatga tacaatggaa aaaataaatt caaaaggagt ttttttatga
    17641 cttatccaaa tctcttggac cgcttcttaa cctacgttaa ggtcaacacg cgctctgatg
    17701 aacactctac tactactcca agtacacaga gtcaggttga cttcgcaaca aatgtcctaa
    17761 ttcctgaaat gaaacgtgtt ggactgcaaa atgtttacta tctaccgaat ggttttgcta
    17821 ttggaacctt gccagccaac gatccgtctt taacacgtaa gattggtttt atatcgcaca
    17881 tggatactgc tgattttaat gctgaaggag tcaatccaca ggtaattgaa aactacgatg
    17941 gtggtgtgat tgaactaggg aattctggtt tcaaactcga tccagctgac ttcaagagtc
    18001 ttgaaaaata tccaggacaa acgctcatca caacagatgg aacaaccttg ctaggtgctg
    18061 atgacaagtc aggaattgct gaaattatga cagccattga atatctaact gctcatcctg
    18121 aaattaagca ctgtgagatt cgtgttggtt ttggtccaga tgaagaaatc ggtgttggtg
    18181 ccaataaatt tgatgcagaa gattttgatg tggattttgc ctacactgtt gatggtggtc
    18241 cactaggtga acttcagtac gagactttct cagccgctgg tgctgaattg catttccaag
    18301 gccgtaatgt ccaccctggt actgccaaag ggcagatggt caatgccctt cagctagcaa
    18361 ttgattttca taatcaactt ccagaaaatg accgacctga gttaactgaa ggttaccaag
    18421 gtttttacca tctaatggat gtgacaggta gtgttgagga ggcgcgtgca agctacatca
    18481 ttcgtgattt tgaaaaagat gcctttgaag cgcgtaaagc atccatgcaa tctatcgctg
    18541 ataagatgaa tgaagaactt gggagcgacc gtgtcactct caacttgaca gaccagtact
    18601 acaatatgaa agaagtcatt gaaaaagata tgactccaat taccattgct aaagccgtta
    18661 tggaagatct aggtatcacg cctattatcg aatcaatccg gggtggaaca gacggctcta
    18721 agatttcctt tatgggaatc ccaactccga atatctttgc aggtggcgaa aatatgcacg
    18781 gacgttttga atacgttagc cttcagacta tggaacgtgc agttgatacc atcattggca
    18841 tcgtagctta taaaggctaa aaagacgagg tggctcagct acttcgcctt tctttttatt
    18901 ctactggttt ttcttgattt ccagtagttg tagaagattc tgttgtttca ttttctgaag
    18961 ttgattcagc aggtttagaa tctcttgtat tgcttggttt gttttcgtcg ctagcagttt
    19021 caatgttaga ttctgcagtt gcgtttggtt ggttctcagc actggtgtta tcaccatttg
    19081 cttcagcatt tcttgctgga cttgtttctt cacttgcgct agcttttgac tggatttgat
    19141 gattcaaaac tagaatagct tttgtcggtt caagtaaagc tgttttgtct ttactcttag
    19201 cagaaagttg atctaataat gcatccacct tatcaaagtc cgcatcagat ccattattac
    19261 tttctaaata agagtgaagc gacatgagaa tatcgtagag tttttgatag agtacaagtg
    19321 tctgaggatc ttgctcagca ttttcctttt cttgttgaag ggcgctagcg atacgagtca
    19381 aaacatcttt tacctgactg tttacttcat ccaagtctgc atcagccttg tttgtggcag
    19441 cttttagatt ttctacttct tctgccaagg attgtctgat tccttcttca tggatttgtt
    19501 ccaagagttg atttgccttg ctcaaaagac tttctacttc ttccttgcta tctgtcgcag
    19561 attattggtt gccatctacc atgtactcct aaaacaggag agttataatc caagattaca
    19621 aggccttaca gaaataagaa atccagataa gacaatgttc gtccaagacg ctattcgctt
    19681 cgcacagcag cacggattca atatgcttta attttaaagt ttaggtgtca agacctcttt
    19741 ttagtgtgcc caaaatttag agaagtaatc aatcaactaa cttttatttt tttcaaactt
    19801 tcagtaaact gacctaaagc taactcaatc tgtctttgtt cgataggctt gtctttgtag
    19861 atgcttctgc tatcagctag aagttgatct acttttgcca agactgcctt ctcataaaaa
    19921 gttccaggtt gatagttgga ttgcagggat ggaatcttgt ttttcaaagc cgcttcatat
    19981 cccttagttt gaaccttgat gtagtgattg tggtcgccac gaggaatcac aaaaccttct
    20041 gaatcttcac ttataattcg attggcatca aaaccatgac catcttcttc ctcatggtgg
    20101 acatgtagtg acggattact taatacagaa ctagaagaac ttcctacctt ttccgtgtta
    20161 gagtgtgatg ggggattgtt aagagatgac ttaggaatat agtgatagtg accccatgtc
    20221 ttactatata agcatcacct gtatctctga caatatcatt agggttaaag acatatgtgg
    20281 ctgctaattc acctgccgac aagtcactct caggaatgaa atgatagtga ccaccatgtg
    20341 gtactatagt aggttgaaat agaatatgag caaattgata aggggatttt aaagtaattt
    20401 ctaacaatga tttagaaact atgatgtgct attctaaatt caactcacta tatataacca
    20461 tcatcggtag tataacgtcc ctgtaatttt gctacagata cttctgcact agctccttta
    20521 tcgtctttac catgttcttg tttttggcga ttgatttcat cttttgttcg tacattttct
    20581 gcatgagctt gatctttaag gtaaacataa tactttccat ctaccttaat aatatatcct
    20641 cccttaacct aactgacaat atcttgatct ttcggctgat agttgggggc tttcattaat
    20701 ggctcttcac taaagagcgc atcaaaagga actttaccat tatagcagtg ataatgatcg
    20761 ccatgagaag ttacataacc ttgacctgta atcttaataa caatttgttt tgcttgaatt
    20821 ccttcttttt gactaaccta gtctggagtc aaattttcag tcttcttagt gtctttatta
    20881 ctgtttacat atgaaacacg atttttatct gtattggcct gttagctatg ttggttcaga
    20941 gcataaacac acagacttaa ggaaaggata acaacagatc cagctgctat atatttcttt
    21001 ttaaatttca taa
//
LOCUS       DAVLYV010000029        20385 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLYV010000029 DAVLYV010000000
VERSION     DAVLYV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20385
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.029"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..3495)
                     /gene="mfd"
                     /locus_tag="VYO70_001068"
     CDS             complement(<1..3495)
                     /gene="mfd"
                     /locus_tag="VYO70_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1713313.1"
                     /translation="MVTLLDLLSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKRKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNK
                     KDYEILEGLLIFGESLLEI"
     gene            complement(3496..4065)
                     /locus_tag="VYO70_001069"
     CDS             complement(3496..4065)
                     /locus_tag="VYO70_001069"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1713314.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            complement(4136..5251)
                     /gene="ychF"
                     /locus_tag="VYO70_001070"
     CDS             complement(4136..5251)
                     /gene="ychF"
                     /locus_tag="VYO70_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1713315.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMKFRFNV"
     gene            complement(5371..6537)
                     /locus_tag="VYO70_001071"
     CDS             complement(5371..6537)
                     /locus_tag="VYO70_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1713316.1"
                     /translation="MKITEYTKKDGSTVYRSSVYLGIDTVTGKKVKTTISGRTKRELK
                     AKALQAQIDFEKGGATVYKAVEIKTYAELVENWLETYCHTVKKSTLMGTKFKIDKYLL
                     PAFGNYRLDKLTPPIIQKQVNQWAKDYNQLGKGFQEYPLLHSLNKRILKYAVSLQAIP
                     FNPARDVIVPRRKEKEGQKLKYLDDDNLKKFLTYLEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLKNGTLDVNKTLNIIKEITGPKTKSSIRVIDLDNKTVLMLRLY
                     KARQSQIGKEMGLTYEKVFSNSFDKHIDARMLSFRLVKHLERAGCPRFTFHAFRHTHA
                     SILLNAGLPYKEIQIRLGHAKLSMTMDIYSHLSKDNQKNATSFYEKAIEKLKSS"
     gene            complement(6633..7085)
                     /locus_tag="VYO70_001072"
     CDS             complement(6633..7085)
                     /locus_tag="VYO70_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1713317.1"
                     /translation="MDKINYFSAEEAILLHDIEVIEKIGGLHGVKDKGRVFSVFEHLQ
                     NDDYYPDFETKLTVLVFNTVQFHMFNDGNKRSSIVFGIKFLTINHLDYLIPFFIETME
                     NIVLWVAMGLIDFNFLKEIISSILYYEELTEKVKEKLVQIALAEENNK"
     gene            complement(7085..8089)
                     /locus_tag="VYO70_001073"
     CDS             complement(7085..8089)
                     /locus_tag="VYO70_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1713318.1"
                     /translation="MTDLTTSQIERQNILNNSLALQKAEEILKVPGFYFEDTFYFTNS
                     QLATFFEVDIRTIERLVEAHKTELTENGYHTLRGEKLAKFKENAFATDTNVGSKVTQL
                     SISSFRTLLNFAMLLTNSDIAKQVRNTLLDIVINVLTEKTGGHVKFINQRDKNYLSTA
                     LEEDNARKIFTNAIDKYVSGSNFKYGQLTNEIYKAIFKERASEYKKILKLNKNDNARA
                     TMYSEVLIVIASFEKGVAYEIEQKSKSSNQKLTNTDVIEIIQRLANHPMQEPHLMHAR
                     EKMASRDLSFRNAKHEKLAEYIRPISEEDFERFIGEQSKSLSEQIDEHRQVFERLRDK
                     "
     gene            complement(8238..9194)
                     /locus_tag="VYO70_001074"
     CDS             complement(8238..9194)
                     /locus_tag="VYO70_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713319.1"
                     /translation="MAKNSPQDLENREYFSKRLNLLMEQKGVRQIDLHNALDIPKSTI
                     TGYVKGKSLPTAGNLQKIADFLNVKKSFLDLRFIDTVSTSTLKELLDNNQREKVFEMF
                     FNKIKEILSFKKDYMNMEYIRGKIAFDQGVLGRVLQSSIINPGIVEIFNGLDLSDISK
                     NNEALLLSKQREAREYAYNNVLTMITNEYITRLINVIEREQALNTSYVHSEISNLINS
                     LNNKIDSYGAVVFEVNEIIEKIGIQDRKILGYCLQRKISSVQNEINDLVHNNTNNSFC
                     VTKNKIFTEKSDIIDTIEFDYYKGIQNRLNDLAEEIREHFSD"
     gene            9351..9584
                     /locus_tag="VYO70_001075"
     CDS             9351..9584
                     /locus_tag="VYO70_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713320.1"
                     /translation="MEQWTLKACRVNAGYTLRQVAKKVNKNFQTVSKYEKDSTYIPFE
                     LLKELSAIYQVKLDNIFLGDSTKKIELNKRELQ"
     gene            9674..10300
                     /locus_tag="VYO70_001076"
     CDS             9674..10300
                     /locus_tag="VYO70_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEV1713321.1"
                     /translation="MELVYMDGKKEPYTLSSIVAECAEVKHRHLKILLNKHREDFESF
                     GKVQFKISPSESGQNVRDYILNEQQATLLITYLRNTEPVKEFKKNLVKAFFEMREELS
                     KFRMQRALEKPKRKTLHDSIKTWEAKPKHAHSTMNNLLLKAVTDMNAKQLVKSRGGYN
                     GIDSLTSDELEQYQAFEDMVIAMIGLNMSYQEIKAMAFRNKKTRQRSV"
     gene            10752..11204
                     /locus_tag="VYO70_001077"
     CDS             10752..11204
                     /locus_tag="VYO70_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713322.1"
                     /translation="MTETTYENLTRRINRISAEMQEIGEKNGLSRLSLLASQTKFIKE
                     ELSRLLWIELPELNDSHKIEAVSKSTTGMFFHPGIFEMDAMRQAFFKRQAMHFFENEA
                     EQQAYIEHAEKEYLEATITLKDILFNSKNGTQRVNKSCLIEKFEEAMQ"
     gene            11201..11401
                     /locus_tag="VYO70_001078"
     CDS             11201..11401
                     /locus_tag="VYO70_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713323.1"
                     /translation="MTLDLDNMTQAEFDKQMAEIKERNPNIFQLISDFLDRKVTPEEV
                     DEFLKMERAEQVEYIKNYQARV"
     gene            11403..11618
                     /locus_tag="VYO70_001079"
     CDS             11403..11618
                     /locus_tag="VYO70_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713324.1"
                     /translation="MNELDISNTQAAVLIVILIGLLLYLNHRDRKKSAQIERENQQTI
                     KTPSEDLNPDYGRYIQLAGVNVWGGME"
     gene            11615..11908
                     /locus_tag="VYO70_001080"
     CDS             11615..11908
                     /locus_tag="VYO70_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713325.1"
                     /translation="MSYTMNLYPDIEKIPDETYFTKEIFLATCLNNTIRIAVARGVLT
                     SQLLDKETKEVVCKTLDTIIENQKAILQGVASGQITFVNKSKGEEGLDESIRE"
     gene            11889..12230
                     /locus_tag="VYO70_001081"
     CDS             11889..12230
                     /locus_tag="VYO70_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1713326.1"
                     /translation="MSLSENDKRVLRLIKVGAENSITGSEISLTTKLTERTVRDIIKR
                     LVVKHNIPIVGVRCGVFSGYFIPANKGELLDGAKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            12223..12498
                     /locus_tag="VYO70_001082"
     CDS             12223..12498
                     /locus_tag="VYO70_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713327.1"
                     /translation="MFSLSRESEQDLAHGILEVVERYLEAREKVPPRLTKLINRVELK
                     EELKISDNTLNKWESQGLRRYQPPDDGARIIFYLVDDIRRFMGVENG"
     gene            12638..13498
                     /locus_tag="VYO70_001083"
     CDS             12638..13498
                     /locus_tag="VYO70_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase alpha helix C-terminal domain-containing
                     protein"
                     /protein_id="HEV1713328.1"
                     /translation="MTIYEARGFQSNLVYPFDKMEPFQYIERFKPLVVPESADPEEYK
                     RTQAPYCLSGKVMPEKNGSYKRNNSSLIYRDLIFLDYDDIQGTTEDFIEAVSSALFGY
                     SYILYPTIKHSIEKPRFRLVVKSNNVMNEATYKQVVKEIADKIGLPFDMASLTWSQLQ
                     GLPVTTGDPATYQKIVEHGLDYPVPKVEPRAKQGTTERYKPRVSGQRSMTMRIIDTLF
                     NGFGDEGGRNVAVTRFVGLLFNKWVDCDLETAYELTKIANSVTVEPLPIEELDRTFSS
                     IARAEYRKRG"
     gene            13551..15020
                     /locus_tag="VYO70_001084"
     CDS             13551..15020
                     /locus_tag="VYO70_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase family protein"
                     /protein_id="HEV1713329.1"
                     /translation="MEQEHPPRTMKELENRIFKAGEEWRAEHTETKVNETTGDVTEKV
                     AMPQTFTVAKILSEIVTFTFISKSNIPDYSLLYIYDLDEGIYTASNDLFNLLCKTFDV
                     RIKPREWPQIKLMVRTLAKIRKPLESANLVPVKNGIIDLRTKELLPFSPKYVITSKIS
                     TAYHAPKRVPTDREGKTFDDWLNSIACNDSELVTLFWQIILEAINPNHTRNKFAIFYG
                     DGNNGKGTFQRFLINLIGESNISALKPAQFGEKHNLETLVGKVCNIGDEAPNEYLKNP
                     SDLMSITSGDTVLVNPKGRPAFEATFKLFNIFSGNYIPNGGNKTKGWYRRIMIVPFNA
                     DFNGEKEKPWIKNEFLANKEVLEYALYKAINQEPFTHFIEPKAVKGLLEEYQEDNDYL
                     LSWVKHEYMEKGWHELDVVPVFIVTRSLKHYAEDMGIPKPNVYGAGKETIKHLQQLTP
                     NRYSLRKKRVPSEDFEKLEAWDVDKIKMRTPQHSITKQE"
     gene            15361..15528
                     /locus_tag="VYO70_001085"
     CDS             15361..15528
                     /locus_tag="VYO70_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713330.1"
                     /translation="MKVKLFEYTRRWEKEDFEKEINDFMATVEVLDVKFSGTPSSESF
                     NSVAIVLVLYR"
     gene            15611..16141
                     /locus_tag="VYO70_001086"
                     /pseudo
     CDS             15611..16141
                     /locus_tag="VYO70_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173118.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16212..16711
                     /locus_tag="VYO70_001087"
                     /pseudo
     CDS             16212..16711
                     /locus_tag="VYO70_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            17387..17797
                     /locus_tag="VYO70_001088"
     CDS             17387..17797
                     /locus_tag="VYO70_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HEV1713331.1"
                     /translation="MTPEQVKEKLEGVKWINKEIEGLYLELAALESGIIKKQELSNTR
                     VQTSRVNTTENNPISVLKLKEDTLQRIERLNEERMEISRLIDKLANPLERSVLRLFYL
                     NVLDAWQVAEEIDKSKTAVYIIRQKAIEHLANVG"
     gene            17953..18345
                     /locus_tag="VYO70_001089"
     CDS             17953..18345
                     /locus_tag="VYO70_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1713332.1"
                     /translation="MELDKFKTMMNVRERMTYFLRFQRMAGSENQVTIDEEAWKLVLP
                     YRWDLSGEHEKAIREGLEIFAQDINSIENKRARKYFIIHYCYMRKKTMSECVEMAATS
                     STSYHRYKQIAVLNFARIHQNGELEVYK"
     gene            18399..18557
                     /locus_tag="VYO70_001090"
     CDS             18399..18557
                     /locus_tag="VYO70_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713333.1"
                     /translation="MIEDFLIDLENIQLEDMEIDFNMDIDIDLNIDFEIDVDLDDLDL
                     TLLQELEA"
     gene            complement(18986..19180)
                     /locus_tag="VYO70_001091"
     CDS             complement(18986..19180)
                     /locus_tag="VYO70_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1713334.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANCWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(19245..20381)
                     /gene="dnaN"
                     /locus_tag="VYO70_001092"
     CDS             complement(19245..20381)
                     /gene="dnaN"
                     /locus_tag="VYO70_001092"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1713335.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
BASE COUNT         6602 a         3691 c         3852 g         6240 t
ORIGIN      
        1 tatctctaat aaactttctc caaaaatcag cagaccttct aaaatttcat aatctttctt
       61 attttggaca tcaaatacaa gctccattaa tcccttattc tcagcgatgc ctgcttttaa
      121 gttcgttacg gataaagctt taaaataatc ttgagctaaa aacaatcgtt gagtgacttt
      181 ttcaaattga attgtaattt tattatcttt tcttttatta tcttttcttt ccacacgttg
      241 aacaaagacc ttgtccaagt atgatttaac caaaccaatc tctaaaagat aggctactac
      301 atctgggtat tctccaaaac ggtctatcaa ctcctcttgt aactcttcat aattgacacg
      361 gttgtcaatt tgacgaattt tcttgtaaat ttcaatctta tgtcgttgat cagaaatata
      421 agtatcagga agataggcat caatttgcaa aatcaactca gcattccctt tggttcttgt
      481 gttagcgtta gcattaccgt ttcgtttagc aatagcttcc tctaataact gcgaatacaa
      541 ttcaaaacca acagaatcaa tgaaaccaga ctgggatttt cctaaaagat ttcctgctcc
      601 acgaatcgaa agatctcgca ttgcaatctt aaagccagag cccaattctg taaatccttt
      661 aatcgcttct aatctctttt cagagacttc actgattgat ttttctggac gatacatgag
      721 ataagcataa gcaatacgat tactacgacc gactcttcct cttaactgat ataaggttga
      781 caagcccata tggtccgcat tttcaataaa taaagtatta gcatttggaa tgtccacccc
      841 tgtctcaata atagtagtcg tcaccaagat atcgtattgt ccctcaataa agtctaatag
      901 agtattttcc aactggactt cactcattcg accatgaaca tatccaatcg aagcctccgg
      961 aatcaactcc tgtaattctg aaaccttctg aacaattgtg tcaactttgt tgtaaagata
     1021 ataaacttga cctccacgct ccatttcacg caagacagca tcacgaatga cactatcatt
     1081 cttttccaaa acataggtct gaacaggata gcgattagtc ggcggagttt caataacaga
     1141 taaatctctg attcccagca tagacatatg gagggtacga gggattggcg tagcggtcaa
     1201 ggttaggaca tccacttgtt tcttcagttc tttcaaagtt tccttatgct tgacaccaaa
     1261 tcgctgttcc tcatcaataa tcatcaagcc caaatcagca aacacaacat cttttgacaa
     1321 aacacgatgt gttccaatca aaatatcgac ttgaccgttt ttcaattttt caagtgttgc
     1381 agtctgctct tttttacttc taaagcgact caacacatca atattaactg caaaattttg
     1441 gaatcgttcc ttaaaattcg tatagtgctg ttgcgctaaa accgtcgtcg gaactagaat
     1501 gacaacctgt ttgtgatcat tgactgcttt aaaggctgca cgcatagcaa cttcagtctt
     1561 tccaaaacca acatccccaa ctaaaagtcg atccattggc tgagaagcct gcatatccct
     1621 cttgatttcc tcaatactac gaagttgatc atccgtttca acataaggga aagcatcatc
     1681 aaaggcatct tgatcatcat catcagctga gaaagcaaaa cccttcaact gactacgttc
     1741 agagtagagt ttgattaaat catcagctat atcctctacc tggttcttaa ccttttgctt
     1801 ggccttttta aaatgaccgt catttaattt attgagtttt ggagctttac catcacttga
     1861 aatatatttg gacagtagat gaatctgttc cacggggata gaaatttgat caccattttg
     1921 gtattggaca ctgacataat cgcgatgaat tcccttgatt tcaatggttt caattcctag
     1981 atattgacca atcccatgga tatgatggac aacatagtcc cctttttcaa gttcattgta
     2041 atcttttaat ctctctgcat ttgaaacatg ttgtcttcga aaacgacgct ttaatttctt
     2101 ttgaaaaatc tcatgttcag ttatcaataa aatcttttca tctacaaaat gaaaaccatg
     2161 tctgagatta ccctctatta agtttacaga ttctttacag atacttgtct tatctctaga
     2221 atccaattta atctgatatt cctctaacat atcctccaat gttttacttc ccattgaatt
     2281 gctagactgc agaataatgg tgtaatccat ttttttatat cgttcaattt cttcttttag
     2341 aaaagaaaac tgattgaaaa attcctgcat aggatattga ttgaattgat aaattttgtc
     2401 aaatttgaga tttcctaaac ccttttgaag attagagaaa aaggtcactg gactttgttt
     2461 tttatagatt tgttcaatat cagaaaaata ctgcatatca gaaaatgctt tactattctg
     2521 taattcttct gtaaagtact gcgctaattc tctttcaaag acttcatact gattcatcaa
     2581 tttttgataa tcatcaaaga atattggagt atctttttca atataatcaa agacagtcca
     2641 tgtcttatca tagcacaaag ataaaaactt ccgagagtct gcatgacttt gtttttggtg
     2701 aaaacttgaa agaatttctt ctaggtatga tttcaaaata ggtgataaag tttttgaaat
     2761 ttgtttttct aaagctgact gtcctcgttg ataatccttt tctctcaaaa gcatatcact
     2821 agctggaaag atagtgagtt ctgtcttatt ttctttcgat aattgtgttt ctacttcaaa
     2881 tgacctgata ccatcaattt catcaccaaa aaactcaatt cgacaaggtt ctaactggga
     2941 tatttcaaaa atatctaaaa tatctcctcg aagactaaat tcgccctgag tttgtacttg
     3001 agtaactttt cgatagccat tttcctttaa ctgatggata aacgcgtgtt gatcatattc
     3061 ttcaccaact gagattttta caatactatc tttgaatgca ttgggagacg gtaaaatcaa
     3121 tcgacttgct gcgatattac aaactaaaat ccctttctta gatgaatcag tcaaaaaacg
     3181 caaggcttca acccgtgaaa taattttttc ctgtgaagac atcaaaaact ccaccatagg
     3241 agcatcatct accaaaaatg gatagacgag ttcctcaccc aagatagata taagatcact
     3301 aacaagtcct tctgcttctc cataagttga cgtcaataac acaatcctat cttctttttc
     3361 taaactgctt gcaattgcaa gagccttagt agatgttgat aaaccaagta ttagttgtct
     3421 tttcttatct gttaaatttt gatgccattt tttaatctga tcattttctg agagtaaatc
     3481 taataaggtc accatttatc cgttatacct ctgcattgtt ttctcaaaat ttttctcttg
     3541 taaatagtag tttacagaat cgtcaacttt gtcaacagac tgtaaaatac cgatataatc
     3601 atccctgtca aacttactca aaacatgatg aacaactgac ataccatttt taggtcttcc
     3661 aattccaatc ttaacacggt taaagacctg agttcctata tgttgaataa tagacttgat
     3721 accattatga ccacctgctg agccttttgc tcttaaacga attttcccaa cttccatgtc
     3781 aagatcatcg taaatgataa gtaaatcgtc aatatccaaa ccatagtaag ttaataaagc
     3841 atgaactgct tttccacttt cattcataaa ggtcgttggt ttaaccagat aaattttttc
     3901 tccatttagg aaaaaggatg ctaggtcagc ttgaaatatc ttatcgtgtg taaaagtgac
     3961 attctgtttc ttcgctagtt gatcaatcaa cataaaacca acattgtgtt ttgtttcaaa
     4021 atatttatcc cctggatttc ccaaacctac aagtaatttg gtcatttatt tttcctttca
     4081 aaagccaaaa gggttggaat ttttttccaa cctaattgac accatttatt aatttttaga
     4141 cattaaagcg gaatttcatg atatcgccat cttgaacgat atattctttt ccttcttcac
     4201 gcaagcgtcc agcttctttt acggcctttt cagatccgta tttcactaga tcttcatatg
     4261 acatggttac tgcacgaata aagccttttt caaagtctga gtggataata ccagctgctt
     4321 gaggagcctt cataccacgt ttgaaagtcc aagcgcgaac ttctttttca ccagctgtga
     4381 agtaagttcc caatccaagc aagtggtaag ctgcacgcgt caacttatct acacctgatt
     4441 ctgtcaaacc aatggcttca agaaactctt ttttatcttc atcatccaat tcagaaattt
     4501 cttcctcagc acgcgcagaa ataacgacta cttcagcatt ttctgtcgct gcaaattcac
     4561 gaatttgttt gacatagtcg atagagtcag gttctgaaac cacatcctcg tccacattag
     4621 ctacataaag aactggttta gtcgtcaaaa ggaaaagacc tttgacaacc ttttgttcct
     4681 catctgtaaa ttcaatggta cgagctgatt tcccgtcttc taggactggt ttaatctttt
     4741 gaagaacatt gaattctgct actgattctt tatctttttg cgtacgtgcc atcttttcta
     4801 cacgcgcata tcgtttgttc actgattcta agtcagcaag aattaattcc agattaattg
     4861 tatcaatatc tgcaagtgga tctacaaagg cgtcttcacg tccttgctcg cgcattacat
     4921 tttcatcatc aaaagcacga actacgtgaa caatcgcatc tacttcacga atattggcca
     4981 agaatttatt ccctagccct tctccttttg aagctccttt tacaatccct gcaatatctg
     5041 taaattcaaa tgttgtggga actgtctttt taggagttat catttcagtt agtttttgta
     5101 ggcgttcatc tggaacttcc accattccaa catttggatc aatagtcgca aatgggtagt
     5161 ttgctgcctc tgctcctgct tttgtaattg cattaaatag tgttgattta ccaacgtttg
     5221 gcaaaccaac gatacctgct gtcaaagcca tatttctcat tctccgtttt catttcaatc
     5281 cctaatatta taacacaaaa agggaaaact tgctaaaatt ccttgatata gctatttccc
     5341 ataactgaaa taataatttt atggactttt ttaggaactt tttaattttt caatagcttt
     5401 ttcataaaat gaagtagcat ttttctgatt gtctttggat aggtggctat aaatatccat
     5461 ggtcatagaa agttttgcat gaccgagccg tatttgtatc tctttgtaag gtagtccagc
     5521 attgagcaag atactagcat gagtgtggcg gaaggcgtgg aacgtaaaac gagggcatcc
     5581 agcacgttcc aaatgtttta ctaacctgaa actaagcatt ctagcgtcta tatgcttatc
     5641 aaagctatta gagaatactt tctcataggt caaccccatt tctttcccta tttgggattg
     5701 tcttgcttta tagagccgta gcatgagcac cgtcttatta tccaagtcaa ttaccctaat
     5761 acttgattta gtcttagggc cagtgatttc tttgataata tttaacgttt tatttacgtc
     5821 tagtgtgccg ttttttaggt caatatcaga ccatttgagg gctaaacact cacgaatacg
     5881 caaaccagtc gctaaaagcg tcttatatag caccgtatcg tagaaatttt tgtaagtgtt
     5941 tggcagctgc tccaggtaag tcaagaattt ttttaagtta tcatcatcca gatatttcag
     6001 tttttgccct tctttttcct tacgacgtgg cacgataaca tcacgggcag ggttaaaggg
     6061 gatagcttgc aaagatacgg catatttaag tatgcgtttg tttagagagt gaaggagcgg
     6121 atattcttgg aatcctttgc ctagttgatt atagtctttt gcccattgat tgacttgctt
     6181 ttgaatgatt gggggcgtca gtttatccag cctataattt ccgaaagctg gaagtagata
     6241 tttatctatt ttgaatttgg tacccattaa ggtcgatttt ttaaccgtat ggcagtacgt
     6301 ttctagccaa ttctccacca gttcggcgta agtctttatc tccaccgctt tatatactgt
     6361 agcccctcct ttttcaaagt cgatttgagc ctgtagcgct ttggctttga gttctctctt
     6421 ggttcgccct gatatagtcg tcttgacttt cttacctgtt acggtatcga taccaaggta
     6481 gacgctagag cgatagactg tagacccgtc ttttttggtg tattctgtta ttttcatgat
     6541 gataaacctt tctaaaatac atcagcaggc aagctattat taaaaaggtt ttagattatg
     6601 gtttatatca tgttgagctt acgggaaaga ttttatttgt tattttcttc agctaaagct
     6661 atctgtacaa gtttttcttt tactttttca gtgagttcct catagtagag aattgaagaa
     6721 ataatttctt ttaaaaaatt gaaatcgatt aaccccattg ctacccataa gacaatattt
     6781 tccattgttt cgataaagaa gggaatgagg taatcaaggt gattgattgt taaaaattta
     6841 ataccgaaga caatgctact tctcttattt ccatcattga acatatgaaa ttgaactgta
     6901 ttaaatacta gcactgtaag ttttgtttca aagtctggat agtaatcgtc attctgtaag
     6961 tgttcaaata cactaaatac ccttccctta tctttaacac cgtgtaaacc acctattttc
     7021 tcaattactt caatgtcgtg tagtaaaatt gcttcttcag cagaaaaata attgatttta
     7081 tccattattt atctctcaat ctttcaaaaa cttggcggtg ttcatcaatt tgttccgaaa
     7141 gtgatttact ttgttcgcca ataaatcttt caaaatcttc ttcagaaatt ggacggatat
     7201 attctgctaa tttctcatgt ttggcattcc tgaaagatag atccctacta gccattttct
     7261 ctctggcatg cattaaatgg ggttcttgca ttgggtgatt tgctaatctc tgaattatct
     7321 cgattacatc agtattagtt agtttttgat ttgaagactt actcttttgt tcaatctcat
     7381 aagcaacccc tttctcaaaa ctagcaataa ctataagtac ctcactatac attgttgcgc
     7441 gagcattatc atttttattc aatttcagta tttttttata ttctgatgct ctttctttaa
     7501 agattgcttt gtaaatttca ttcgttaatt gaccatattt aaaattactt ccagagacat
     7561 acttatcaat ggcatttgta aatatttttc tagcattatc ttcttctaat gcagtcgata
     7621 gataattttt gtcacgctgg tttataaatt ttacatggcc accagtcttc tcagttaaca
     7681 cattaatgac aatatcaagt agggtatttc ttacttgctt agctatatca ctattggtta
     7741 acaacatagc aaaattaagc aatgttctga acgatgagat acttaattgt gtaactttgc
     7801 taccgacatt tgtgtcggta gcaaaagcat tttctttaaa tttagctagt ttttctccac
     7861 gtaaagtgtg ataaccattt tcagtaagct ccgttttatg agcttcaaca agtctttcaa
     7921 tagttcgaat gtcgacttcg aaaaaggtag ctagctgaga atttgtaaaa taaaaagtat
     7981 cctcaaagta gaaaccaggt acttttaaaa tctcttcagc tttttgtaat gctaaagaat
     8041 tatttaaaat attttgtctt tctatttgtg aggttgtcag atcagtcata atttctcctt
     8101 tttgtcgagt taatttgcca ataactcccg atatttcaag agttttattt tattttcgtg
     8161 tcgttagagt atattagaaa atcttgaact ccgcgaatac acgcgcttca ggattgttga
     8221 acattgtggc gcgtgggtta gtcgctgaag tgttccctga tttcttcagc taaatcattc
     8281 aagcggttct gaataccttt atagtaatca aattcaatgg tgtcaatgat gtcacttttt
     8341 tcagtaaata tcttgttttt tgtaacacaa aagctattat ttgtattatt gtgaacaagg
     8401 tcatttatct cattttgaac agaagatatt ttcctttgta agcaatagcc taagattttt
     8461 cgatcttgaa tacctatttt ttcaattatt tcattcactt caaagacaac tgccccatag
     8521 ctatcaattt tattgttaag gctattgata agattagaaa tttcagaatg cacataggag
     8581 gtattgagag cttgctcacg ttcaataacg tttattaaac gtgtaatata ttcgttagta
     8641 atcatggtga gtacattatt ataagcgtac tccctagctt cgcgctgttt ggaaagtagt
     8701 agagcctcat tattttttga gatatctgat aaatcaagtc cgttgaaaat ttcaacaata
     8761 ccagggttta ttattgaaga ctgaagaacg cgacccaaaa caccctggtc aaatgctatt
     8821 tttcctcgaa tgtactccat attcatataa tcttttttaa agctaagtat ttctttgatt
     8881 ttattgaaga acatctcaaa aaccttttct ctttgattat tatctagcaa ctcttttagt
     8941 gtgctggttg aaactgtatc aataaaccgc agatctagga aagatttttt tacgttcaaa
     9001 aaatcagcta ttttttgtaa attcccagcg gttggtaaag attttccttt tacatatccc
     9061 gtaattgtac ttttaggaat atctaaagcg ttatgaaggt caatttgtct aacccctttt
     9121 tgttccataa gaaggttgag ccgtttagag aagtattctc tattttcaag atcttgagga
     9181 ctatttttag ccatgacttt aaccctttca gttgtaagtt atacattgat tataacacaa
     9241 aataaaaaca aagtacgaaa ataaataaac ttttttctaa aaacacttga ccaagtacga
     9301 aaataatagt actattaact tatcactttt gaaaggaatt gttagattat atggaacaat
     9361 ggacgttgaa agcctgtaga gtaaacgctg gttatacttt gcgacaggtt gctaaaaagg
     9421 taaataagaa ttttcaaact gtctccaagt acgaaaaaga tagtacatat ataccgtttg
     9481 aacttttaaa agaattgtct gcaatttacc aagttaaatt ggacaatatt tttttaggag
     9541 atagtacgaa aaaaatagaa ctaaataaaa gagagttgca ataatgagag tattatttta
     9601 agtcaatggt ggcagaaatc aaaccagaca taacaataga tgaattattt tatagctaga
     9661 aaggagcaaa ccaatggaac tagtctatat ggacggtaaa aaagagccgt atacactgag
     9721 cagtattgtg gcagagtgtg cagaagtcaa gcacagacat ttgaagattt tgctaaataa
     9781 acaccgagag gacttcgaga gtttcggaaa ggtgcaattt aaaatttcac cttcagaaag
     9841 tgggcaaaat gtacgggact atattttaaa tgaacaacaa gcaacattgc tgatcactta
     9901 cttacgaaat acagaacccg taaaagagtt taagaagaac ctagtcaaag cattctttga
     9961 aatgcgtgag gaactttcta agtttcgtat gcagagggcg ctagaaaagc caaaaagaaa
    10021 aaccttgcat gacagtatta aaacatggga ggcaaaaccc aagcacgcgc atagcaccat
    10081 gaacaacctg ttactgaagg cagtaactga catgaacgct aagcagttag taaaaagccg
    10141 tgggggctat aatggcattg atagcttgac cagtgacgag ctggagcaat accaggcatt
    10201 tgaggatatg gtaatagcca tgattggctt gaatatgagt tatcaggaaa tcaaagctat
    10261 ggcattcaga aataaaaaaa cacgccaaag aagcgtgtga gagcaacaaa aaaggcttag
    10321 caataaccac accgcagagc ctttcacact aacactaaaa cgaatttaac aaaaagcagg
    10381 caagctatta tcaaagaggt tttagtaaag atttatacct agattatagc atatttaggg
    10441 caatttgacc atacggagag cgccaactct ttaaactggt actttctcac gctttcaatt
    10501 tggcgagtct gagcgtgagg ctagtactta caagaaaaaa ctgtataaga aacaactcaa
    10561 aaagccccac gctcagaagt ttggcgaccg agagcatgag gctgtaggca agaaaaactg
    10621 tataagaaac aaccattcaa aatccccaca ctcgccatcg ctcaactttg agtgtgagga
    10681 tcagcaggta tagtaaaagg tattaaatgg cccttagggc aactacacaa catagaaaca
    10741 gaggtaaaaa catgacagaa acaacatacg agaatttaac tagacgtata aacagaatta
    10801 gcgcagaaat gcaagaaatt ggcgaaaaaa atgggcttag tagactttcg ttattggcaa
    10861 gtcagacaaa attcatcaaa gaagaattat cccgtttact ttggattgaa ctccctgaat
    10921 tgaatgacag ccataaaatc gaagcggtct ctaaaagcac tacgggaatg tttttccacc
    10981 ctggtatttt tgaaatggac gctatgcgac aagcattctt taaacgccaa gctatgcact
    11041 tttttgagaa cgaagcagag caacaggcgt atatagaaca tgctgaaaag gagtatttag
    11101 aggctactat aaccttaaaa gatattcttt ttaactctaa aaatggaact cagagagtaa
    11161 ataaaagttg tcttatagag aagtttgagg aggcaatgca atgacactag acctagacaa
    11221 catgacacaa gcagaatttg ataaacaaat ggctgaaatc aaggagagaa atccaaacat
    11281 ttttcaactg atttctgatt ttttagatag aaaggtaaca cccgaagagg tggatgagtt
    11341 tttgaagatg gagcgagctg aacaagtgga atatattaag aattatcaag cgagggtata
    11401 acatgaatga gctagatata agtaacacac aggctgctgt cttaatcgtg attttgattg
    11461 gcttactgct ttatttaaac caccgagacc gcaaaaaaag cgcccaaatt gagcgagaaa
    11521 accaacagac gataaaaaca cctagcgagg atttaaaccc tgattatggg cgatatatcc
    11581 agcttgcagg agtcaacgta tggggaggga tggaatgagt tacacaatga atttataccc
    11641 cgatattgaa aaaataccag atgaaaccta ttttactaaa gagatttttc tagcaacgtg
    11701 cttgaataac acaatcagaa tagcagtagc cagaggagtg ctaacatctc aacttttaga
    11761 caaagaaact aaagaagttg tttgtaaaac cttggataca atcatagaaa atcaaaaagc
    11821 tattttgcaa ggggtagcga gcggacaaat aacctttgta aataaaagca aaggagagga
    11881 ggggttggat gagtctatca gagaatgaca agcgagtttt aagactaatc aaggtagggg
    11941 ctgagaactc cataacaggg tcagaaatta gcctgacaac caagctaaca gaaagaactg
    12001 tacgtgacat tatcaagcgt ctagtagtta agcacaacat ccctattgta ggggttagat
    12061 gtggcgtttt tagtgggtac tttattccag cgaataaagg cgagttacta gatggcgcta
    12121 aggcttttta caaccaagta caggaggaga gcaagcgcct agcggtgttg atgaatagcg
    12181 acctagaaag ctacaaagaa accttgaagg aggtgggcgg atatgtttag cctaagccga
    12241 gaaagcgagc aagacctagc gcatggcatt ctggaggtag tggaaagata cctggaagcg
    12301 cgtgagaaag taccgccaag attgaccaag ttaataaata gagttgaact caaagaggaa
    12361 ttgaaaatta gtgacaacac gctgaataag tgggaaagtc agggtttaag acgctatcaa
    12421 ccgccggacg atggtgctag aataatattt tatttagtgg atgacattcg taggtttatg
    12481 ggggtggaga atgggtagat ataacacaca cccagcgcaa ggcggaggtc attatcatga
    12541 cattaaattg tataagcacc gtggcagatc tttggaacag ttccaagaac aaaagctact
    12601 gaagaagtta aaaaaaaacg caaaaagggg cgatagtatg acaatttatg aggctagagg
    12661 tttccagagt aatcttgtct atccatttga taagatggag ccgttccagt acattgaacg
    12721 ctttaagcct ttggtagtcc ctgaaagtgc tgatccggag gaatacaaaa gaactcaggc
    12781 gccatattgt ttaagtggta aggttatgcc ggagaaaaac gggagttaca aacgaaacaa
    12841 ctccagtcta atctatcggg atttgatttt ccttgattat gacgatatac aggggacgac
    12901 tgaggacttc atagaagccg tttctagcgc tttgtttggc tactcttata tcttataccc
    12961 gactatcaaa catagcatag aaaagccccg ttttcgcctt gtggtgaaat ctaacaacgt
    13021 gatgaacgag gcaacctata agcaggtagt aaaagagatt gctgacaaga ttgggctacc
    13081 ctttgacatg gccagtttaa cctggtcaca actccaaggg ctaccagtaa caacaggcga
    13141 cccagcaact tatcaaaaga ttgtagagca tggacttgat tatccagtcc ccaaggttga
    13201 accaagagcg aagcaaggaa ctactgagcg ttataaacct agagtaagtg gccagaggtc
    13261 aatgactatg aggatcattg acacgctttt caacggattt ggagacgagg gagggcgtaa
    13321 cgtggcagtg acacgctttg tgggattact cttcaataag tgggttgatt gtgacctaga
    13381 gaccgcctat gaactgacaa agatagccaa tagtgtgaca gttgaaccat tgcctattga
    13441 agagctagac cgcactttta gcagtatagc acgggctgag tatagaaaga gaggttagaa
    13501 ccatagaact agaagaatta gcaaacctaa aaagtgaaat catggaggta atggagcaag
    13561 aacaccctcc tagaacgatg aaggagcttg aaaatcgtat ctttaaagct ggagaagaat
    13621 ggcgggcaga acatactgaa actaaggtaa atgagaccac aggggacgtt accgaaaagg
    13681 ttgccatgcc tcagacattc acagtagcaa aaatactgag cgagattgtc acatttactt
    13741 ttatcagcaa gagcaatata cctgattata gcctgctcta tatctatgat ttagacgagg
    13801 gtatctatac cgctagtaat gatctattca atcttttgtg taagaccttt gacgttagaa
    13861 ttaaacccag agagtggcca cagattaagt taatggttag aacattggca aagatacgaa
    13921 aacctttaga gagtgctaac cttgtacctg taaagaatgg cattattgac ctaagaacta
    13981 aggagctact cccctttagt cctaagtatg taattacaag taagataagc acggcctacc
    14041 acgcgcctaa acgagtccca acagatagag agggcaaaac ttttgatgat tggctaaact
    14101 caatcgcttg taatgatagt gaactggtaa cactgttttg gcagattatc cttgaagcta
    14161 ttaaccctaa ccatacccga aataagtttg ctatcttcta cggggacggt aacaatggta
    14221 aagggacatt ccagcgcttc cttatcaatc tgatagggga aagtaatata tcagccttga
    14281 agcctgcaca gttcggagaa aagcataact tggagacctt ggtaggtaag gtttgtaata
    14341 ttggggatga agcgcctaac gaatatttaa aaaatccgtc tgatttaatg agtatcacta
    14401 gtggcgacac tgttctagtc aatccaaaag gacgcccagc ctttgaagct actttcaagc
    14461 tattcaatat cttttcaggg aattatattc ccaatggtgg caacaagaca aaaggctggt
    14521 atcgtagaat tatgatcgtc ccttttaatg ctgactttaa cggggagaag gaaaaacctt
    14581 ggattaaaaa tgagttttta gcaaacaagg aagtcctaga gtatgccctc tacaaagcta
    14641 tcaatcaaga gccatttact cactttatcg aacctaaagc agttaaagga ctcctagaag
    14701 aataccagga ggataatgac tacttacttt catgggttaa gcatgaatac atggaaaaag
    14761 gttggcatga gctggacgta gtacctgttt ttatcgttac aagatcacta aagcactatg
    14821 ccgaagatat gggaataccc aaacccaatg tttacggggc tggtaaagaa actatcaagc
    14881 acttacaaca gttgacacct aatcggtatt ctctaagaaa gaaaagagtt ccttcagaag
    14941 attttgaaaa actagaggca tgggacgttg acaagataaa aatgcggaca cctcaacact
    15001 ctatcactaa acaagaataa gtgtgacgtt ctttttttgc ttaaaccctt gataatactg
    15061 attttcttaa aaaaagtgtg acgttctcaa aaaagaaggt tacacccctt gaagccttga
    15121 tatatctagc tttaggggaa taggtgtgac gttgtgacat tctttccaac tcttatatat
    15181 agagaaaaat gcagtattta tatataggaa cgataaaaca aaagaacgtc acagggtcac
    15241 aggggtggtg aaacacctag agcctcaagg gtttgaacgt gtgacgttct ttgtcacaga
    15301 aggtcacaga acacgaaaaa tattacataa tttaccttaa aataaagaaa tgacaacaac
    15361 atgaaagtta aactatttga atatactaga cgatgggaaa aagaagactt tgagaaagag
    15421 atcaatgact tcatggcaac tgttgaagtg ctggacgtta aattttctgg tacaccatct
    15481 agtgaatcct ttaattcggt ggctatcgta ttggtattat acagataaca aaatggggta
    15541 gatagtaccg acccccttgt aatggtgtaa ctactagtga cacctttaaa acagaaagag
    15601 gaaaaacaac atgacattaa aaacattttc ggataaagcc aaaacattta ctttcactta
    15661 cgaatttaaa gacctagata ctgctatggt agcaggtcac gcgctactgg gatatatgac
    15721 tgggacttat gaagtaccat ctatttcaat aactcacaag gataaaggga cgcttgtcgc
    15781 tgagtatgtg gaagatcaca agctaaacaa gactttcaag cgtatttgtg acagtttcaa
    15841 agactattac aaccaaccag tggacgatga agcatttgaa gaacgttata aacgggagcg
    15901 cgtgctccaa ctcaaagagt aagaggactt tgagaacttg ctggaaaaag taacagacta
    15961 cgagctggaa ctattagact atgctgatcg cttgctttct gataagccta tccctatgga
    16021 ctctatgacc gcttttggta cgttggaaat gctgggcgat gaaagtatta gcctactcca
    16081 aaagttggac gtagagggcg aatataaagg ccttgctggt tatacagagc atttgaagta
    16141 gaacacaggg gagcaatccc ctttttgtta tcaagtcaat agttttgggt ttttccatat
    16201 agggggaaat aatgaagcgg ttagcaatag aaacaatcac taaaccaatg aagctgagag
    16261 gaatatctaa gggtatagct gagttggacg gtcaacgttt agagattgat ttggataatt
    16321 taatgattga ttttggtgga gagtcatttg aattagacag aatagcagga actaagggag
    16381 gtaatagata tttctttgcc ctgattgtgg gagacggtgc aggttgcttt ataaacggta
    16441 tttatatttt agttgcggta cttgtctaga tatccacaag agcaccctga accgtagtaa
    16501 aacggattgc caatactact gggagcttgc actaaaagaa gccagaaagg tagaaccagg
    16561 gtggagtcct agacgtggtg gatatatgtt tgatagtttc cctgaaagac caaagtatat
    16621 gaagcgtgac aggtatcata agcactataa gaagtttttg aagtatattg aaaaaggaga
    16681 tagattttgg ctgaatggtt tgagattgta ggacaatatg aagtagtgtg caatttacaa
    16741 aacaaacaaa tactacatat ttgacggttt gaatgttaaa aaagctagat atatcaagga
    16801 tttaagtaag tagagtgttc caaaagaggg tgaggtaagg gtgaggttac atataaaaag
    16861 tacaaggtta gtacaaggtc acatacttca aaatagcaag gttttagcaa ggtacagggc
    16921 ttttttctag tgatagaacc ctaatattac cctaaggtat agctactttt ttagaaccat
    16981 agaacggtaa ggtatcgaag ttttccaatg gtgaggtaag ggtgaggtaa gagcaaaaaa
    17041 tgtaaaggtt ttgtcaaggt tgcgtattta aaagccccaa ggttaccaca aggtagagag
    17101 gtttaccgag ggttagaaaa ggtgagattt tgcttaggta gtggcagtaa atatgcctgg
    17161 aaaatggcag ggttatgtgt gttgaactcc gagcgaacgc cgagaaagtg gctatatctc
    17221 cctccccacg cacttctatg agcttcacac cgtcattgta cattttttct cacggggaag
    17281 catttgaagc tagagcgaac atagagaaaa cgaaaaaagc caagacactc tctgtctcag
    17341 ctaatagttc tcacaaagac tattatatca tagaggaggc cgaggcatga caccagagca
    17401 ggtaaaagaa aaactagagg gcgttaagtg gataaacaaa gagatagaag gcttatattt
    17461 agagcttgca gctttagaaa gtggtattat caaaaagcaa gaactaagca ataccagagt
    17521 acaaacaagc agggtaaata cgaccgagaa taaccctata agtgttctaa aactaaaaga
    17581 ggatacactt cagagaattg agcgacttaa tgaagagaga atggaaatat ctaggctaat
    17641 cgataagcta gccaatccgc ttgaacgttc tgttctcagg ctgttctact tgaatgttct
    17701 tgacgcttgg caagttgctg aggaaataga taaatcaaag actgcagtat acattataag
    17761 gcaaaaagcc atagaacact tagcaaatgt ggggtaatag gtgagcacaa aaaaacgcct
    17821 agaacaatct aagcgctaaa gagtagtgga cggtgtgcct gtcccgtcat ctcatactat
    17881 gaagttgcgt agcgacacta tcatctctac ctcacttctc tttagactat tataccacaa
    17941 aggagcgaac taatggagct ggataagttt aaaacgatga tgaacgtcag agagcggatg
    18001 acctactttc tacgtttcca gaggatggca ggaagtgaaa accaagttac gatagatgaa
    18061 gaggcttgga aacttgtctt accttatcgg tgggatttga gcggtgagca tgaaaaagca
    18121 atccgtgagg ggttggaaat attcgcccaa gacatcaaca gtatagaaaa caaacgagct
    18181 agaaaatact ttattatcca ttactgttac atgagaaaga aaacaatgag cgaatgcgta
    18241 gaaatggcag ctactagctc cactagctac caccgatata aacaaatagc cgtcttaaat
    18301 tttgcgagaa tccaccagaa cggagagcta gaagtgtata agtaggcgtt tgtggtgttt
    18361 tgttgagaaa tgttgagatt taagtgaggg gggatgacat gattgaagat tttttgattg
    18421 atttggagaa tatccaatta gaggatatgg agatagattt taacatggat atagacatcg
    18481 atttaaacat agattttgaa atagatgttg accttgatga tttagattta accttactac
    18541 aagaattaga agcgtaaatg ttggcaaatg taggctgagg cttcatattc gtcccctatt
    18601 ttgctttggt ttggactgga caattaaaac accatggata aactagaagc cctcagaaac
    18661 gagtctaggg gcttttgtga ggtcataatc ttataaacca cgcgcttcat ggtataatat
    18721 acctatcagc aaccagcaat aaaaaaagca cgtttaaccg tgctagtttc ttgcctgctg
    18781 aactcgtcat ttattgccct tttgtggggc tttttttgtg gactttttga gaatttttaa
    18841 gaacttttaa tgatttctaa tcaaatgaga tttttcaaaa agtcagtaat atcaagggtt
    18901 taggcgcttt aaacaagttt aaaagcgtat agattacaat ctaacacaaa aagggataaa
    18961 gtgctaaccc tctaactaag ggttctcagt caataatttt attcattttt cgctcaaaat
    19021 catatcgccc catcatgaca acatgctcac aattactgca ttttattttg atatctgctc
    19081 ctacacgtgt aatttcccaa caattagcct ttttaccagt cgacttgatt gtacaagcgt
    19141 gtgatttttt catctcaaca aaatttccaa cttgatacat actactctcc ttttcttttt
    19201 cgattatatc ataaaagagg cgagccaggc tcaacctctt tcacttaatt tgtacgaact
    19261 ggtgtaatga gctgcatgaa gtcttcgtca gtatctgctg gcacaagagt aaatggacga
    19321 acagctgaga taaagctaat ggtcaccttt tcgctattta aagctttaag agaatcaatc
    19381 aagtaagttg ggttgaaact aatggtcaaa tcttcaccag taacctgatc agtatcgatt
    19441 tcttcgttta ctttaccaac ttctggagag tgaacatggg cgctaacaac cccatcctta
    19501 atttcaagtt tcacagtacc attttgagtc gcacttgata aaagacgggc acgctccatt
    19561 gactggcgta agtttaccac attaaaagta atagtagtgt taaagtctgt tggaatcaag
    19621 cgatctgtat caggatagtt tccttctagg agacgagtat agaagctaat attttcgctt
    19681 ctaaagagga tttggttatt ggcaaagaaa atctctacag tttcgatatc atctgtaaat
    19741 accgctgaaa attcgcgtag agaacggcta ggaattacga catcaaaatc atcactattt
    19801 ttttcaagag tcaatttttt ctggcttagg cgatgagagt ctgttgcaac tgtttttaac
    19861 tctttgtgtt gactcaatac gaagtggaca cctgttaaaa tcggacgact ctcttgtgta
    19921 cttgcagcaa aggctgtttc attaataatt ttcttgagta attttgtttc aagtattaaa
    19981 ggagtgcttg ctgaaatttc ttggattcgt ggatattgtt cgctatcttt tccttttagg
    20041 gtaatttctg atttgccact ggttaaaaca atttgatttt gttcaatttc tttaaaatca
    20101 agagttacat caggtaaact agatactaca ttgataaaga aagaagcttc aagaaggatc
    20161 gaacctaaag aagtaattaa caaaccagca tcttcatttt tttgagaaat aaaattttca
    20221 attgaaattt gaccatttga accaattaaa gtaatacctt cattggtcac gtcaattttt
    20281 actgttgata aaataggaat ggcattttta gaactaatag ctctcttagt agtatttaat
    20341 gcttgtagaa ataaattttt attaattgaa aaatgaatca tggat
//
LOCUS       DAVLYV010000030        19827 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLYV010000030 DAVLYV010000000
VERSION     DAVLYV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19827
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.030"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            167..2095
                     /gene="glgB"
                     /locus_tag="VYO70_001093"
     CDS             167..2095
                     /gene="glgB"
                     /locus_tag="VYO70_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1713336.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKNFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNPKGVENEK"
     gene            2085..3227
                     /locus_tag="VYO70_001094"
     CDS             2085..3227
                     /locus_tag="VYO70_001094"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1713337.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            3217..4356
                     /gene="glgD"
                     /locus_tag="VYO70_001095"
     CDS             3217..4356
                     /gene="glgD"
                     /locus_tag="VYO70_001095"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1713338.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            4353..5786
                     /gene="glgA"
                     /locus_tag="VYO70_001096"
     CDS             4353..5786
                     /gene="glgA"
                     /locus_tag="VYO70_001096"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1713339.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            5845..6216
                     /locus_tag="VYO70_001097"
     CDS             5845..6216
                     /locus_tag="VYO70_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1713340.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            6161..6490
                     /locus_tag="VYO70_001098"
     CDS             6161..6490
                     /locus_tag="VYO70_001098"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1713341.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            6499..7614
                     /locus_tag="VYO70_001099"
     CDS             6499..7614
                     /locus_tag="VYO70_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1713342.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     VSGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGILDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(7611..8057)
                     /locus_tag="VYO70_001100"
     CDS             complement(7611..8057)
                     /locus_tag="VYO70_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1713343.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            8221..9525
                     /gene="eno"
                     /locus_tag="VYO70_001101"
     CDS             8221..9525
                     /gene="eno"
                     /locus_tag="VYO70_001101"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1713344.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            complement(9662..10108)
                     /locus_tag="VYO70_001102"
     CDS             complement(9662..10108)
                     /locus_tag="VYO70_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1713345.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETTD
                     KNWHKSDRIFVTNTRKPAHSSILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(10008..10854)
                     /locus_tag="VYO70_001103"
                     /pseudo
     CDS             complement(10008..10854)
                     /locus_tag="VYO70_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            11025..11180
                     /locus_tag="VYO70_001104"
                     /pseudo
     CDS             11025..11180
                     /locus_tag="VYO70_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            11380..14655
                     /gene="rexB"
                     /locus_tag="VYO70_001105"
     CDS             11380..14655
                     /gene="rexB"
                     /locus_tag="VYO70_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1713346.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLEYLSQQASFSITVTRFAQMARYLVLNDLPAKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQIALVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFFEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            14652..18302
                     /gene="addA"
                     /locus_tag="VYO70_001106"
     CDS             14652..18302
                     /gene="addA"
                     /locus_tag="VYO70_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1713347.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGCQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQITILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            18517..19695
                     /locus_tag="VYO70_001107"
     CDS             18517..19695
                     /locus_tag="VYO70_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713348.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKNGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
BASE COUNT         6004 a         3798 c         4277 g         5748 t
ORIGIN      
        1 gcgttttcgc aattttttga aaaaatattg atttacaatt gaacgagtaa gtgaacttgt
       61 tgtagatttt caaaataatt aaagaagtat agtaatttac ttgaatcttc tctaaaaagg
      121 tagtataata aaagcataga aaacgcttac aggagaaggg gagtatatgg ataatagaga
      181 agcattaaaa acctttatga cgggtgaaaa tttttatctc caacattatc taggagcaca
      241 tagggaagaa ctaaatggag agcatggcta taccttccgt gtttgggcac ctaatgctca
      301 ggctgttcac ttggttggtg attttaccaa ctggattgaa aatcagattc caatggtaag
      361 aaatgatttt ggggtctggg aagtctttac caatatggct caagaagggc atatttacaa
      421 atatcatgtc acacgtcaaa atggtcatca actgatgaag attgaccctt ttgctgtcag
      481 atatgaggct cgtccaggaa caggggcaat cttaacagag cttcctgaga agaaatggaa
      541 ggatggactt tggctggcac gaagaaaacg ttggggcttt gaagagcgtc ctgtcaatat
      601 ttatgaagtt cacgctggat catggaaaag aaattctgat ggcagtcctt atagttttgc
      661 ccagctcaag gatgaactca ttccttatct cgttgaaatg aactatactc atattgagtt
      721 tatgcccttg atgtcccatc ctttgggctt gagttggggg tatcagctta tgggttactt
      781 cgctttagag catgcttatg gccgaccaga ggagtttcaa gattttgtcg aggagtgtca
      841 tacccataat attggggtta ttgtggactg ggtaccaggt cactttacca tcaacgatga
      901 tgccttagct tattatgatg ggacaccgac ttttgaatac caagaccata ataaggctca
      961 taaccatggt tggggtgccc ttaattttga ccttggaaaa aatgaagtcc agtccttctt
     1021 aatttcttgc attaagcatt ggattgatgt ctatcatttg gatggtattc gtgtggatgc
     1081 tgttagcaac atgctctatt tggactatga tgatgctcca tggacaccta ataaagatgg
     1141 cggaaatctc aactatgaag gttattattt ccttcaacgc ttgaatgaag ttattaagtt
     1201 agaatatcca gatgtgatga tgattgcaga agaaagttcg tctgcaacca agattacggg
     1261 aatgaaagag attggtggtc taggatttga ctacaaatgg aacatgggct ggatgaatga
     1321 tatcctccgt ttctacgaag aagatccgat ttatcgtaaa tatgacttta acctggtgac
     1381 tttcagcttt atgtatgttt tcaaggagaa ttatctcttg ccattctcgc acgatgaagt
     1441 ggttcatggc aagaagagta tgatgcataa gatgtgggga gatcgttaca atcaattcgc
     1501 aggcttgcgc aatctctata cgtaccaaat ttgtcaccct ggtaagaaat tgctcttcat
     1561 gggtagcgaa tacggtcaat tcctagaatg gaaatctgaa gaacagttgg aatggtctaa
     1621 cctagaagac ccaatgaatg ctaagatgaa gtatttcact tctcagctaa accagtttta
     1681 caaagatcat cgctgtctgt gggaaatcga taccagctat gatggtattg aaatcattga
     1741 tgcggataat cgagaccaga gtgttctttc ctttattcgt aagggtaaaa agggagaaat
     1801 gttagtctgt atctttaaca tggtacctgt tgagcggaaa aattttacaa tcggactacc
     1861 cgttgcagga atttacgaag aagtatggaa tactgagttg gaagagtggg gaggcgtttg
     1921 gaaagaacat aatcaaactg ttcaaacgca agaaggacta tggaaagatt atgagcagac
     1981 cttaaccttt accctaccgg ctatgggagc aagtgtatgg aaaatcaaac gtcgcttgaa
     2041 atctactaaa accgtcacaa ataaaaaccc aaaaggagta gaaaatgaaa aatgaaatgt
     2101 tagctttgat tcttgctggt gggcaaggaa ctcgtctcgg taaactcact caaagcatcg
     2161 caaaaccagc tgtgcaattt ggtgggcgct accgtatcat tgactttgcc ctatcaaact
     2221 gtgccaactc agggattcat aatgttgggg tcgttacaca gtatcaacca cttgctctca
     2281 acaaccatat tgggaatggt tcaagctggg gactagacgg tattaattca ggtgtctcta
     2341 ttcttcaacc ttattctgca agtgaaggaa atcgttggtt tgagggaact agtcacgcta
     2401 tttaccaaaa tatcgactat atcgacagtg tcaatcctga gtatgtcttg attttgtctg
     2461 gggaccacat ctacaaaatg gactatgatg atatgctcca gtctcataag gataataatg
     2521 ccagcttgac agtagcagtt ttagacgtcc ctcttaaaga agcaagccgt tttggtatca
     2581 tgaacacaga tgctaacaat cgtattgttg aatttgaaga aaaaccagct caacctaaat
     2641 ctacaaaagc ttctatggga atctacattt ttgattggca acgccttcgt aatatgttag
     2701 tcgctgctga aaagagcaag gttggcatgt cagactttgg taaaaatgtc attccaaatt
     2761 accttgagtc aggtgaaagt gtttatgctt acgaatttag tggttattgg aaagatgttg
     2821 gtactattga gtcactttgg gaagcgaaca tggagtatat ttctccagaa aatgccttgg
     2881 atagtcgtaa ccgtcaatgg aagatttact caagaaactt gatttcacca ccaaacttcc
     2941 tcggggcaaa tgctcatgtg gaagactcat tagttgtaga cggatgtttc gttgatggaa
     3001 ctgttaaaca ttctatcctt tcaacaggcg cgcaagttcg cgaaggagcg gaagtccttg
     3061 attcagttat catgagtgga gctatcattg gtcaaggagc taagattaaa cgtgccatta
     3121 ttggtgaagg tgcgattatt tctgacggtg tcgaaattga tggaacagat gaagtacaag
     3181 ttgtaggata taatgaagta gtgggggtag caacagatga agattgataa atattctgcc
     3241 attttaggaa atacagttgg ttttcacaat atgtcgacat tgacggacca ccgtccagta
     3301 gcaagtttgc catttggtgg gaaatatcgt ttgattgact tcccactttc aagccttgct
     3361 aatgcaggtg ttcgtagtgt ctttggtatt ttccagcagg ataatatcag ctcagtattt
     3421 gaccatattc gttcaggacg cgagtggggc ttatcaaccc ttcttagcca ttactatcta
     3481 ggaatttaca atacccgtgt agaaagtagt acagttggaa aagaatacta ccaacagctt
     3541 cttacttatt tgaaacgttc tggctcaaac caaacggttg ccctcaactg cgatgttctt
     3601 attaacattg atttgaacca agttttccac ctacatagta caacaaaaga gcctatcact
     3661 gtagtttata aaaaactagc taagaaagat atttcagaag taaatgcaat cttggatgta
     3721 gatgaaacag accatgttct ttctcataaa ctctttgata gcaagtcaac agctgaaacg
     3781 ttcaatatgt ctacagatat ctttgtcgtt gatacacctt ggttgattga acacttggaa
     3841 gaagaagcta aaaaagaaca tccagagaaa ttgcgctatg ttttacggga tttggctgta
     3901 aaagagggag ctttcgccta cgagtacacg ggctatctag ccaatattca ctctgttaaa
     3961 tcttattatc aagcgaatat cgatatgctt gaatcacaaa aattctattc tcttttctca
     4021 ccaaaccaaa agatttatac aaaggtcaaa aacgaagagc caacttacta tgctaataca
     4081 tctaaggtaa gcacttctca gtttgcctct ggtagtatta ttgaaggtca agtagctaat
     4141 tctgttctat cacgtaatat tcatgtccat aaggatagct tggttaaaga tagcctgctc
     4201 ttccctcgtg ttgttattgg agaaggggct caggtcgaat atgctatctt ggacaaaggg
     4261 gttgaagttg agcctggtgt tgtgattcga ggaactgcag aacatccagt tgtggttaag
     4321 aaaggtgcta aagtaacaga ggatattcat tcatgaaaat tttatttgta gcagcagagg
     4381 gtgcaccctt ttcaaaaaca ggtggtttgg gagacgtcat tggcgctctt ccaaaatcac
     4441 tggtaaaagc tgggcacgaa gttgcagtga ttttacccta ctatgatatg gtagaggcta
     4501 aatttggaaa tcagattgaa gatgtgcttc attttgaggt gagcgttggt tggcgcagac
     4561 agtattgtgg aattaagaaa acagtattaa atggtgtaac cttctacttt attgacaatc
     4621 aatattattt cttccgtggt catgtttacg gtgattttga tgacggagaa cgctttgcct
     4681 ttttccaact ggctgccatt gaggctatgg aaaggattga ctttattcct gatcttctcc
     4741 atgttcatga ctaccataca gctatgattc ctttcttgtt gaaggaaaaa taccgttgga
     4801 ttcaagccta tgaggacatt gaaacagttt taaccattca taatttagaa ttccaaggac
     4861 aattttcaga aggaatgttg ggtgatttgt ttggagttgg ccttgaacgt tacgctgatg
     4921 gcacccttcg atggaacaac tgtctgaact ggatgaaggc aggtattctc tatgcgaacc
     4981 gtgtttcaac cgtttcacct agctatgctc atgaaattat gactagtcag tttggatgta
     5041 atttggatca gattcttaaa atggagtctg gtaaagtatc tggtatcgtg aatgggattg
     5101 atgctgatct ttataatcct cagacggatg ctcttttaga ctatcatttc aatcaggaag
     5161 atttgtctgg gaaagccaaa aataaggcaa aattgcaaga aagagttggc ttgcctgtta
     5221 gagcagacgt tccactggtg ggaattgttt ctcgtttgac acgtcaaaaa ggttttgatg
     5281 tggtggtcga aagtcttcac catatcttgc aagaagatgt tcagattgtt cttttgggaa
     5341 ctggcgatcc agcctttgaa ggagctttct catggtttgc tcagatttac ccagacaagc
     5401 tatcaacaaa tatcactttt gatgtcaaac ttgctcagga aatctacgct gcttgtgacc
     5461 tcttcctcat gccaagtcgt tttgaaccgt gtggcttgtc tcaaatgatg gctatgcgtt
     5521 atggaacctt gccattggtc catgaagttg gaggcttgcg agatacagtt cgcgctttca
     5581 atccaatcga aggaagcggt actggcttta gctttgacaa tctatctcct tattggttaa
     5641 attggacttt ccaaacagca ttggacttgt atagaaacca tccagacatt tggagaaacc
     5701 tacaaaaaca agctatggag agtgacttct catgggatac agcctgcaag tcataccttg
     5761 acttgtacca tagtttagtt aattaataga taaaatcgta tgatgacttc atacgatttt
     5821 taggctgttt agagaggaga actgatgtct caagtaaaag gcttgtgtgt tttggatgta
     5881 gatggaacct taatcctaga agaagtgatt gatcttttag ggagagaggc aggtcatgag
     5941 gcggaaattt cgcagattac aagtcgagca atgcgaggag agttggtctt tgaaagcagt
     6001 ttaagaaaaa gagtgtcctt gttggaaggt cttcctattt tggtctttga taacgtcttc
     6061 aactcaattc atctatcgct aaatgtccca gagttcatct ctattctcca aaagaatggc
     6121 atcctagttg gtctggtgtc tggtggattt acaccaatag ttggagagat tagcaaaatc
     6181 ccttggtatt gcctatttca ctgccaacca gcttgaagtc aaagaaggtc ttttaacagg
     6241 aaaattagtt ggacaaatta taagtcccca ggtcaaaaaa gaaactctgg aaaaatggag
     6301 aaagaaacta aaactttcta aagaaagaac ggtggcaatc ggtgatgggg tcaataatct
     6361 attaatgttg aagtcggcgg agttaggaat cgccttttgt gccaaagaag tgctcaaaaa
     6421 agaaatacca catcatgttg acaagaggga ttttttagaa gttcttcctt tgattgactg
     6481 tttagaatga gggaaaatat gaagattgta attgcaccgg attcgtttaa ggaaagcttg
     6541 acagctcaac aggtagctga agcaataaaa agaggcttcc aacaatcgat agcagatgta
     6601 gaatgtctcc tctgccctgt tggtgatggg ggagaaggta ctgtagatgc tatccgacat
     6661 tctcttgacc taaaagaaaa atggatccaa gtgacagacc cttttggaca aaaagaagcc
     6721 atgcgctatt ttcaaaaagg ggaactggca ctatttgaag tagctgactt ggttggtctt
     6781 ggaaaaattc cgctagagaa acgaaatcca cttcaaatcc aaacttgtgg tattggagag
     6841 ttgattctcc atctcatttc taaagggatt aaagatattt atatcggtgt tggtggcacg
     6901 gccagtaatg atggaggact ggggattgct gctggtttag gttatcaatt ttatgatagg
     6961 gatggaaatg tcttgcctgt ttccggtcaa tccttattaa acttagcttc tgtttcaaca
     7021 gaaaattgct ataaaattcc tgaaggtgtt caaattcata ttctagcaga tgtcgtgagt
     7081 cccttatgtg gtcatcaagg tgcgacttac acttttggca atcaaaaagg tctacatccg
     7141 actatgtttg cagtcgtaga tcaggcgatc caagattttt atgaaaaatt ctcacctgca
     7201 acattagaaa ttaaaggagc aggagcaggt ggaggccttg ctggtggttt gtgtgccttt
     7261 gctcaggcaa gtatcgtgtc tggaattgat acctgcttgg acttaatcaa ctttgataag
     7321 aaagtttcag atgctgactt ggttgttgtt ggagaaggaa tacttgacag tcaaagcttt
     7381 gctgggaaag cgcctatagg tgttgcaaaa agaacccctg ttggagttcc tgttattgct
     7441 atttgcggta gtcttgctga agatttacct cccctaccat ttgaaaatat ccaagctgtc
     7501 ttttctattt tggagaaaag tgaaccttta gaagatagtt tgaaaaatgc cagtctctat
     7561 ctggagcgca cggctgccaa tattgggcac ttattaaata tgtgcaagat ttagctaaac
     7621 catttcttcc ataaggatgt tttggctggt tcttcctttt taacttccca aagttttgga
     7681 aaagcaagtc gaaggtcttt ttcttctact tgaactggtg cattgctatg aataaccagg
     7741 ccgaaaggag aagtaatatg ctcttcagat actatggtgg cttgactatc agtttctttt
     7801 gcttctttta agtagaaaac ttgcttatca aattcgatag ttggtgaaat cttcacaaat
     7861 agtggctctg ctttttcctg aaggttttct aaaataaata aaaagccttt ttctaactga
     7921 gggctatttg ctgtgtcaat atctacatat ccaagaactc tttcctcaaa agtaccaaga
     7981 tagcgtcttt gctcatccgg atttattttt aacccaccat gagctttttc aagtaattgt
     8041 tttgataaat ctgtcatgaa aacagtatat cataaaagtt agaataaaac agacaaaaga
     8101 aagcgattac tttataaagt taaggaaaag ttgcacaaag ataacgtttt ttcttgaaaa
     8161 aggaggggtt ttaaggtatt atagaggtgt aaaaaattta actcataagg agagtaaaaa
     8221 atgtcaatta ttactgatgt ttacgctcgc gaagtcctag actcacgcgg taacccaaca
     8281 cttgaagtag aagtttacac tgaatcaggt gctttcggac gtggtatggt tccatcagga
     8341 gcttctactg gtgaacacga agcagttgaa cttcgcgacg gtgacaaatc tcgttacggt
     8401 ggtcttggta cacaaaaagc tgttgacaac gtaaacaaca tcattgctga agctatcatt
     8461 ggctacgatg tacgtgatca acaagctatt gaccgtgcta tgatcgcact tgacggtact
     8521 cctaacaaag gtaaattggg tgcgaatgca atcctcggtg tgtctatcgc tgtagctcgt
     8581 gctgctgctg actaccttga aatcccactt tacagctacc ttggtggatt caacactaaa
     8641 gttcttccaa ctccaatgat gaacatcatc aacggtggtt ctcactctga cgctccaatc
     8701 gctttccaag agttcatgat cttgccagtt ggtgcgccaa catttaaaga agcccttcgt
     8761 tacggtgctg aaatcttcca cgctcttaag aaaatcctta aatcacgtgg tttggaaact
     8821 gccgtaggtg acgaaggtgg attcgctcct cgtttcgaag gaactgaaga tggtgttgaa
     8881 actatccttg ctgcgattga agctgctgga tatgtaccag gtaaagacgt atttatcgga
     8941 tttgactgtg cttcatcaga attctacgat aaagaacgta aagtttacga ctacactaaa
     9001 tttgaaggtg aaggtgctgc tgttcgtaca tctgcagaac aaatcgacta ccttgaagaa
     9061 ttggttaaca aatacccaat catcactatt gaagatggta tggatgaaaa cgactgggat
     9121 ggttggaaag ctcttactga acgtcttggt aagaaagtac aacttgttgg tgacgacttc
     9181 ttcgtaacaa acactgacta ccttgcacgt ggtatccaag aaggtgctgc taactcaatc
     9241 cttatcaaag ttaaccaaat cggtactctt actgaaactt ttgaagctat cgaaatggct
     9301 aaagaagctg gttacactgc tgttgtatca caccgttcag gtgaaactga agattcaaca
     9361 atcgctgata tcgcagttgc aactaacgca ggacaaatca agactggttc actttcacgt
     9421 acagaccgta tcgctaaata caaccaattg cttcgtatcg aagaccaact tggtgaagta
     9481 gctgaatatc gtggattgaa atcattctac aaccttaaaa aataaaatag ttcagtgaac
     9541 tattttatcc cgaaccatga aattcaaaag ttcggccgtt gatttaacaa cgtttctagc
     9601 ccctcggatt ttatccgaag ggctattttt gtatttagag gacaaaaaag ggacaaaact
     9661 tttaaaaaat ctttttcata actttatcca gtacattgat tgcttcatct ttcatgttct
     9721 ttgtgacgtg ggtgtagatg gaagtagtga cttcagagtc ggaatgacca accctgtccg
     9781 tgattgtttt taaaggaatt ttattttctg ataagatgct aatagtggtg tgtctgaaga
     9841 tgtgagggga cagatgtttg ggaattggtt ttttaaggcg ttcgtttgct cgctggagag
     9901 acttactcaa gatagaacta tgagctggtt ttcttgtatt ggtaacaaaa atacggtcgc
     9961 ttttgtgcca attcttatcg gttgtttcgc ttagacaatt tatatagtca attgaaacaa
    10021 gaacaagaca aaagagcctc gtaaaaggta ttgcaacttg gtaatacctt tttgaggtgc
    10081 tttttgatat gagcccatgt tttctcaata ggattgtact caggcgagta gggaggaaga
    10141 ggtaaaagtt tatgcccaaa ctcttcgcat aaaagttcta gattccccat tctatggaat
    10201 cttgcattat ccataataat aaccgatggt gtggttaatg ttggtaagag aaatttctga
    10261 aaccaagctt caaaaaagtc gctcgtcatc gtctcttcgt aagtcattgg agcgattaac
    10321 tcaccatttg ttagacctgc aaccaaagaa atcctctgat atcttcttcc agatactttg
    10381 cctcttatta actgatcttt taatgagcga ccatattctc gataaaaata agtatcgaat
    10441 cctgtttcgt caatctaaac aggtgctagg tgctttaaac tattaaaatt cttaagaaat
    10501 aaggctactt tttctgggtc ttgttcatag taggtgtggt tctttttttc gagtgtagcc
    10561 catagctttg agcgcatagt ggatggtagt tggatgacag ccaaagtcag aagctatttc
    10621 agtcaaataa gcgtctggat tgtcagtaag atagttttta agtctatctc tatcaacttt
    10681 tcttggtttt gttcctttta cttggtggct tagctctcct gttttctttt ttagctttaa
    10741 ccagccataa atggtattac gtgagatttg gaaaacgtgt gatgcttctg ttatactacc
    10801 tgttcgctca caataagaga gaactttttt acgaaaatct attgaatatg ccataagaag
    10861 attataccac attgtgtcct atttttggtt cattttacta tagtagattg aaataagata
    10921 cgaacagctt gattagggaa atcaaattaa tttctagaaa tattttagca tccacggtgt
    10981 actgttatag tttcaattta ctatagttca atcattctat caagaatatt gaatatataa
    11041 aggcacgtaa tattaaaact tcttctgata tcgaattaga gtccgatgtt gatggcgata
    11101 aatcagacaa tctacccgtc gacattaaga tactgggaca acacattgaa gtgtattccg
    11161 tacctaagaa acaatcataa aactcaaagc cttgttaccg aggctttttt attatgtgat
    11221 tttcagtagc cagacttatt ctatctaata caagtaaagc taagtattta agatttagca
    11281 gatgatgaag aaaagaaagg atggcctaaa tattttaaca gatttacgag gctaatcatg
    11341 gtataatata gggtgatgaa aaattccaac gaggctgaga tgaaattact ttatactgat
    11401 attcggactt ctttgacaga aattctaaca agagaggcag aagagctagt tgcagctggc
    11461 aagcgggtct tctacattgc ccccaactct ctttcttttg aaaaggaacg cgccgtgctg
    11521 gaatacttgt cccagcaggc ttctttttcg attaccgtca cgcgctttgc tcaaatggct
    11581 cgctatctgg tcttgaatga tttaccagct aaaactactc ttgatgatat cggtcttggg
    11641 ttggcctttt acaaatgcct tgccgaactc gatcccaagg acttgcgtgt ttatggcgct
    11701 attaagcagg atcctcaatt gatccagcag ttaattgagc tttaccatga gatgaccaaa
    11761 tctcagatga gttttttgga cttggagaat ttaacagatg aggataagag ggcggattta
    11821 ctcttgattt ttgagaaagt aacagcctat cttaatcaag gtcagttagc ccagggaagt
    11881 cagttgtccc atttgattga ggctattgag aatgacaagg taagtagtga ttttaatcaa
    11941 atcgccttgg tcattgacgg ctttactcgt ttttctgctg aggaagagcg ggttgtggac
    12001 ttacttcacg gcaaaggtgt tgagattgtt atcggggctt atgctagtaa gaaagcctat
    12061 accagtcctt ttagcgaggg caatctctac caagccagcg taaaatttct ccatcatctg
    12121 gcttctaaat accaaacgcc tgctcaggac tgttctcaaa ctcatgagaa gatggatagt
    12181 tttgacaagg cctctcgttt gttggagtct tcttatgact tttcagaact cgctttggat
    12241 gtcgatgaga aagaccgtga aaatttacaa atctggtctt gtttgacgca aaaggaggag
    12301 ttggagctag tagcccgtag tattcgtcag aaattacatg agaactcaga cctgagctac
    12361 aagcattttc gtattctctt gggggatgta gcttcttacc agttatctct caaaaccatt
    12421 tttgaccagt atcaaattcc tttttatctt ggtagaagcg aagccatggc tcatcatccc
    12481 ttgactcagt ttgtcgagtc tattttagct ttaaaacgtt accgttttcg tcaggaggat
    12541 ttgattaatc ttcttagaac tgatttgtat actgacctca gtcagtctga tattgatgct
    12601 tttgagcaat atatccgcta tcttggtatc aatggcttgc cagcctttca gcaaaccttc
    12661 accaaatccc accatggaaa atttaatctt gagcgtttga atgtcctccg cctgagaatt
    12721 ttagcacctc ttgaaaccct ctttgccagc cgaaaacaaa aggctgaaaa cctcctacaa
    12781 aaatggagtg tctttctaaa agaaggagct gtgaccaagc agttacaaga tttgacaacc
    12841 actttggaag ctgtagaaca ggaaagacaa gccgaagttt ggaaggcttt ctgccatgtt
    12901 ttagaacaat ttgcgactgt ttttgctggt tcacaggtta gtctggaaga cttcctagcc
    12961 ttgctccatt ctggaatgag tttgtcccaa taccgtacca ttccagcaac agtggacact
    13021 gttctggtgc agagttacga tttgattgca ccattgactg ctgactttgt ctatgctatt
    13081 ggactaactc aggacaattt accaaaaatt tctcaaaaca ccagtcttct gacagatgaa
    13141 gaaaggcaaa atctaaacca agcgaccgaa gaaggcgttc aattactgat tgccagcagt
    13201 gaaaatctca agaaaaatcg ctacactatg ctttccttgg tcaattctgc tcgtaagcag
    13261 ttgttcttgt cggctccaag cctttttaac gaaagtgaaa gtaaggaatc tgcctatctt
    13321 caagagttga tccattttgg atttaggcgg agagagaaga ggatgaatca caaaggactg
    13381 tctaaggagg atatggggtc ctatcacagt cttttgtcta gtctggttgc ctatcaccag
    13441 cagggtgaga tgagcgatac tgagcaagat ttgacttttg tcaaggttct gtcgcgtgtc
    13501 ataggtaaaa aactagatca gcaaggtctg gaaaatccag ccatcccaat cagtccaagc
    13561 agcaagacct tagccaagga caccttgcaa gctctctatc ctgccaaaca ggagttttac
    13621 ctgtctacgt cgggtttgac agagttttat cgcaatgaat acagttattt cctacgctac
    13681 gttttaggct tgcaggagga attacgtttg cgtcctgatg cccgtagtca cgggaatttc
    13741 ttgcatcgta tctttgaacg cgccttgcag ttgcctaatg aagattcctt tgaccaacgt
    13801 ctagaacaag ctattcaaga aaccagtcaa gaacgcgaat ttgaagctat ttatcaagaa
    13861 agtttggaag cccagtttac caaggaagtt ttgcttgatg ttgcacggac aactggacat
    13921 attctccgac acaatccagc catcgaaacc atcaaagaag aagcaaattt tggtggaaaa
    13981 gaccaagcct ttattcaatt agacaatgga cgcagtgtct ttgtacgagg caaggtggac
    14041 cggattgacc gtttgaaagc taatggagcg ataggagtag tagactacaa atccagtctg
    14101 actcagttcc agtttcctca tttctttaat gggctcaatt ctcagttacc aacctatctt
    14161 gctgccctaa aaagagaagg ggagcagaac tttttcggcg ccatgtactt ggaaatggct
    14221 gaacctgtcc aatctctgat ggcggtaaaa agtctggcag gagcagtggt agaagccagc
    14281 aaatctatga aataccaagg gctcttcttt gaaaaagaaa gcagttattt aggcgaattt
    14341 tataacaaaa acaaggctaa tcaactgaca gatgaggaat ttcagctcct actggactac
    14401 aatgcctatc tttacaagaa agctgctgag aagattttag caggccggtt cgccatcaat
    14461 ccttatactg aaaatggcag aagcattgcc ccatacgtcc agcaacatca ggctattaca
    14521 ggctttgaag ccaattacca tctgggccaa gcccgtttcc tagaaaagtt ggacctagct
    14581 gatggcaagc gtctggtcgg agaaaaactc aagcaagctt ggcttgaaaa aataagagag
    14641 gagttgaatc gatgaagctt attccctttt taagtgagga ggagattcaa aaactgcaag
    14701 aagcagaagc aaattcgagc aaggaacaga agaaaactgc cgagcaaatc gaagctatct
    14761 acacttctgg ccagaatatc ctggtctcag catcggctgg ttctggaaag acctttgtca
    14821 tggcagagcg cattctggac caattggcgc gtggtgtcga aatttctcaa ctctttatct
    14881 caacctttac cgtcaaggct gcaactgaac ttaaagaacg tttagagaaa aaaatcagca
    14941 agaaaatcca agaaacagat gatgtcgatc tcaaacaaca cttgggttgc cagttggcag
    15001 acctacccaa cgctgccatc ggaaccatgg actctttcac acaaaaattc cttggcaaac
    15061 atggttatct gcttgatatt gcacctaatt tccgtatttt acaaaaccaa agcgagcaac
    15121 ttcttctcga aaacgaagtc tttcatgagg tctttgaagc gcattaccaa ggtaaacaga
    15181 aagagagctt tagtcatttg ctgaaaaact ttgctgggcg tggcaaggac gaacggggtc
    15241 tgcgccagca agtctataaa atctatgact tcctccaatc caccagtaat cctcaaaagt
    15301 ggctgagtga atctttcctc aaaggctttg agaaagctga ttttaccagt gaaaaagaaa
    15361 aactgactga gcaaatcaaa caagccctct gggatttgga aagctttttc cgttaccatc
    15421 tggataacga tgccaaggag tttgcaaagg ctgcctattt agaaaatgtt caattaattc
    15481 tggatgaaat tggctcccta aatcaggagt tcgatagtca ggcttatcag gcagtgcttg
    15541 cgcgtgttgt cgccatctct aaggagaaaa acggtcgagc tctgactaat gccagccgta
    15601 aggctgattt gaagcccctg gctgatgcct acaacgaaga gagaaagacc cagtttacta
    15661 aactaggaca attatcagac cagataacca ttctcgacta tcaagaacgt tatcatggag
    15721 acacttggaa actagctaaa accttccaat ctttcatgag cgattttgta gaggcttatc
    15781 gtcagagaaa acgacaggaa aatgcctttg aattcgctga tatcagccat tacaccattg
    15841 agattttaga aaatttccca caagttcgtg agtcttatca ggagcgcttc catgaagtca
    15901 tggtcgatga gtatcaggat accaaccata ttcaagaacg gatgctggaa ttgttgtcta
    15961 atggccacaa tcgctttatg gtgggagata tcaagcaatc catctatcgt ttccgtcagg
    16021 cagacccgca gattttcaat gagaaattcc aacgctatgc gcaaaatcct caagaaggca
    16081 agctcattct cctcaaggaa aatttccgta gtagttcaga agtgctgtca gcaaccaatg
    16141 atgtctttga acgtctcatg gaccaagagg tcggcgaaat caactatgat aacatgcacc
    16201 agcttgtttt tgccaatacc aaactgactc ccaatccaga caacaaggcc gaatttctcc
    16261 tctacgacaa ggacgataca ggtgaggaag aagagagtca aacagaaacg aaactaacag
    16321 gcgaaatgcg cttagttatc aaggagatcc ttaaacttca tcaggaaaaa ggtgttgcct
    16381 ttaaggaaat tgcccttctg acctctagtc gtaatcgtaa tgaccaaatt ctacttgccc
    16441 tgtctgagta cgggattcct gtcaaaactg acggagagca aaacaattat ctccaatccc
    16501 tagaagtgca agtcatgcta gacactcttc gtgtcattca caatcccctg caagactacg
    16561 ccttggttgc ccttatgaag tctccaatgt ttggttttga tgaggatgag ctagcacgtt
    16621 tgtcccttca gaaagcagag gataaagccc acgaaaatct ctatgagaaa ctggtcaatg
    16681 cacaaaaaaa ggcaagtagt caaaaaggct tgattcacac agctctagct gaaaaactaa
    16741 agcaattcat ggatatccta gcttcttggc gcttgtatgc caaaccccac tctctctatg
    16801 acttgatttg gaagatttac aacgaccgtt tttattatga ctatgttggg gctttgccga
    16861 atggtcctgc taggcaggcc aatctctatg ccctagcact gcgtgctgat caatttgaaa
    16921 agagcaattt caaaggtttg tcgcgtttta ttcgtatgat tgaccaagtc ttagaagccc
    16981 agcacgattt ggcaagcgtg gccgtcgcac cgccaaaaga tgcagtagag ctcatgacca
    17041 tccacaagag taaagggctg gagtttcctt acgtctttat cctcaatatg gatcaagatt
    17101 tcaacaagca ggactctatg tcagaagtca ttctcagtcg tcagaatggt cttggtgtca
    17161 aatatattgc caagatggag acaggggcag tagaagacca ctatcctaaa accatcaaac
    17221 tctccattcc tagtctgacc tataggcaga acgaagagga attacagcta gcaagctatt
    17281 ctgagcagat gcgtttgctg tatgttgcta tgacgcgggc tgagaaaaag ctctatcttg
    17341 tcggcaaggg ttctcgtgaa aagctggaag ctaagcaata cccagcagcc aaaaatggga
    17401 aactaaatag caatactaga ctgcaagcac ggaatttcca agattggctt tgggctatca
    17461 gtaaagtgtt tactaaggac aagctcaact ttagttatcg ttttattggc gaagatcagt
    17521 tgaccagaga agctatcgga gagttggaaa ccaagagtcc tctccaagat agctcccaag
    17581 cagacaatcg tcagtcagat accatcaaag aagctctgga aatgctgaag gaggtggaag
    17641 tttataatac tcttcaccgc gcagctattg aacttcctag tgttcaaacc ccaagtcaaa
    17701 tcaagaaatt ctacgaacca gttatggata tggaaggtgt cgagattgct ggtcaaggtc
    17761 agtcagtagg caagaaaatc agcttcgatt tgccagattt ttcaaccaaa gaaaaggtaa
    17821 ctggagctga gattggtagt gctactcacg aactcatgca gataattgac ctcagccagc
    17881 aactaaccct tgctagccta acagaaacac tcaaacaagt tcaaactagc caagctgtca
    17941 gagacaagat caatcttgat aaaattcttg ctttctttga cacagcactc ggtcaggaaa
    18001 ttcttgctaa taccgaccat ctctatcgcg agcaaccttt ctccatgctc aaacgagacc
    18061 aaaagagtca ggaagacttt gttgtccgtg gtatccttga tggctatctg ctttacgaaa
    18121 acaaaattgt tctgttcgac tacaagacag accgctatga tgaaccaagt caactcgtag
    18181 accgctatcg tggtcagtta gctctatacg aagaggcttt atcacgagcc tattcgattg
    18241 aaaatattga aaaatacttg attttactcg gtaaagacga ggttcaagtt gtaaaagtat
    18301 aacttatact cttcgaaaat ctcttcaaac cacgtcagct tcgccttgct gtatatatgg
    18361 ttactgactt cgtcagttct atccacaacc tcaaaacagt gttttgagct gacttcgtca
    18421 gttctatcta caacctcaaa acagtgtttt gagctgactg cggctagctt tctagtttgc
    18481 tctttgattt ttattgagta ttagaaagga gacctcatga cacttccagt tagaaaatcc
    18541 ctgcacgatg cagttttaca ggcttcgaaa gcggatactt gggaacaagc taccaaggaa
    18601 tggaatgaag tttccttgat ttttaacggt attggccgta gcaattgtgt ctgtggaaat
    18661 gccatcaaat acgcttatga actctttaac ggtgtcacag gccaacgcct ctttccaatt
    18721 ggtagcgact gtgttcgtca ttttcatcga ttgtcccttg accagcaatt ggaagaggaa
    18781 gaaaaactgc tcagaaaggt tgaaaatcta accagaaaag ctcagaaaaa ggaaaaaatc
    18841 aaggtcaata aaagtgactt tgacgagcga cttctaaaat ggctctggga aaaaggtgtt
    18901 ttcaaaccca atcgtggcaa tcaatttgcg cctgagagag actaccagct tttcctagaa
    18961 gtctttcagg gaggaagttg gaccaaggcg gaaccaaaga aaaaggctcg gatggaagaa
    19021 gtccttgaaa agtgtatcaa acccttttta cttgggaaat cggatgacca actctacctt
    19081 gtcaagctag gcaaggagaa aattgactac gaacaagaac tccgtatcca ggcagagaaa
    19141 gaacgtaaga agagggataa aatcgccaag caatatgcag acaatctggt tctagccatg
    19201 ggacccgcag agcgtgctta tcaagattac tttggcttta cagagaccct gactcaagaa
    19261 gaacgcaagt gggaaaaaat tctttttggt aaaaatagag cagaacgggc tatcaaggcc
    19321 aaacaatacc aaaaagagct ggaaaaagac caacgaattg ccagtcagga tccaattgaa
    19381 agaaaacaga agcagacttg gctcctcaat tcctattttc gtgagcttcc tgaagaaaaa
    19441 gctcgatttt ctaggctctt attagaatat cgaaaaaatg gtgaagtacc cttttcaact
    19501 gaatacctgt cagatcatct catcgacttt ttctataaaa tgaaagcctt tgaatttgaa
    19561 atcgcaccag aacaagtccg agattttcta aaaaaaagcc tgcaggaaga ccatcgctca
    19621 tcagcacaag gaagctggat agaaggaatt ctcctcaact gcctcaaacc atttttagaa
    19681 cgattagtga tatgagtaac acctaccgtg taaatttcat ggtaggtgtt attgtacccc
    19741 caaataatat aaaacaaagt atgaaagcgc atctcaattt taagactttt ttgtaaatta
    19801 agagtataat taaggtatac tgccttt
//
LOCUS       DAVLYV010000031        19799 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLYV010000031 DAVLYV010000000
VERSION     DAVLYV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.031"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            22..1503
                     /locus_tag="VYO70_001108"
     CDS             22..1503
                     /locus_tag="VYO70_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1713349.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSP
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            1865..2866
                     /locus_tag="VYO70_001109"
     CDS             1865..2866
                     /locus_tag="VYO70_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1713350.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLKKVEQ
                     AIKTLNYIPDYYARGMKKNRTETIAIIVPSIWHPFFSEFAMHVENEVYKRNNKLLLCS
                     INGTNREQDYLEMLRHNKVDGVVAITYSPIEHYLTSGIPFVSIDRTYSDIAIPCVSSD
                     NDAGGREAAKQLISKGCQHLAFVGGHNTTINETKRRRISFEKYVQEHKIPLSIFDLDE
                     TVTDYYGKLESFLEENSSIDGIFTINDFTALDVIEILKKNGKRIPHDVQIIGYDGIKM
                     AGDRDYLLSTIEQPLEEMAKEAVRILFDIIDGKTVNLQTILPVKFVEGKTTKNENKS"
     gene            3016..3933
                     /locus_tag="VYO70_001110"
     CDS             3016..3933
                     /locus_tag="VYO70_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1713351.1"
                     /translation="MNSKAKQVSLWERIKKQKLLLLMTVPGLVLTFIFKYIPMYGVLI
                     AFKDYNPLKGILGSDWIGFSEFTKFISSPNFGILLANTLKLSIYGLLLGFLPPIILAI
                     MLNQLLSEKVKKRIQLILYAPNFISVVVIVGMIFLFFSVGGPINNFLSMFGMKADFLT
                     NPDFFRPLYIFSGIWQGMGWASTLYTATLVNVDPALVEAARLDGANIFQRIWHIDIPA
                     LKPIMVIQFVLAAGGIMNVGYEKAFLMQTSLNLPTSEIISTYVYKVGLVSGDYSYSTA
                     VGLFNAVINVVLLVAVNQIVKRMNNGEGI"
     gene            3944..4834
                     /locus_tag="VYO70_001111"
     CDS             3944..4834
                     /locus_tag="VYO70_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1713352.1"
                     /translation="MKNSIMDTKFDRRILLLNKIIIVFIVLMTLLPLLYIVVASFMDP
                     KVLVSRGISFNPADWTVEGYQRVFSDQSILRGFINSLLYSFGFAALTVLLSVFTAYPL
                     SKKDLVGRRWINYFLIVTMFFGGGLVPTYLLVKELGMLNTPWAIIVPGAVNVWNIILA
                     RAYFQGLPEELVEAAVIDGANDLQIFFKIMLPLAKPIMFVLFLYAFVGQWNSYFDAMI
                     YIKDPNLEPLQLVLRKILIQSQPGQDMIGAQAAMNEMKRLAELIKYATIVISSLPLIV
                     MYPFFQKYFDKGIMAGSLKG"
     gene            4863..6479
                     /locus_tag="VYO70_001112"
     CDS             4863..6479
                     /locus_tag="VYO70_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1713353.1"
                     /translation="MKFKTFSKSAVLLTASLAVLAACGSKNTASSPDYKLEGVTFPLQ
                     EKKTLKFMTASSPLSPKDPNEKLILQRLEKETGVHIDWTNYQSDFAEKRNLDISSGDL
                     PDAIHNDGASDVDLMNWAKKGVIIPVEDLIDKYMPNLKKILDEKPEYKALMTAPDGHI
                     YSFPWIEELGDGKESIHSVNDMAWINKDWLKKLGLEMPKTTDDLIKVLEAFKNGDPNG
                     NGEADEIPFSFISGNGNEDFKFLFAAFGIGDNDDHLVVGNDGKVDFTADNDNYKEGVK
                     FIRQLQEKGLIDKEAFEHDWNSYIAKGHDQKFGVYFTWDKNNVTGSNESYDVLPVLAG
                     PSGQKHVARTNGMGFARDKMVITSVNKNLELTAKWIDAQYAPLQSVQNNWGTYGDDKQ
                     QNIFELDQASNSLKHLPLNGTAPAELRQKTEVGGPLAILDSYYGKVTTMPDDAKWRLD
                     LIKEYYVPYMSNDNNYPRVFMTQEDLDKIAHIEADMNDYIYRKRAEWIVNGNIDTEWD
                     DYKKELEKYGLSDYLAIKQKYYDQYQANKN"
     gene            6489..7808
                     /locus_tag="VYO70_001113"
     CDS             6489..7808
                     /locus_tag="VYO70_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1713354.1"
                     /translation="MGDKKYTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKNNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDNQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(8017..8089)
                     /locus_tag="VYO70_001114"
     tRNA            complement(8017..8089)
                     /locus_tag="VYO70_001114"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8054..8056),aa:Lys,seq:ctt)
     gene            complement(8354..9625)
                     /locus_tag="VYO70_001115"
     CDS             complement(8354..9625)
                     /locus_tag="VYO70_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1713355.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9895..10134
                     /locus_tag="VYO70_001116"
     CDS             9895..10134
                     /locus_tag="VYO70_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713356.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            10298..10477
                     /locus_tag="VYO70_001117"
     CDS             10298..10477
                     /locus_tag="VYO70_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713357.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSVPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            10614..10793
                     /locus_tag="VYO70_001118"
     CDS             10614..10793
                     /locus_tag="VYO70_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1713358.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERLITIPHGE
                     LNKYTERGIRKQAGL"
     gene            10830..11282
                     /locus_tag="VYO70_001119"
     CDS             10830..11282
                     /locus_tag="VYO70_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1713359.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            11573..12043
                     /locus_tag="VYO70_001120"
     CDS             11573..12043
                     /locus_tag="VYO70_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1713360.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            12062..13150
                     /locus_tag="VYO70_001121"
     CDS             12062..13150
                     /locus_tag="VYO70_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1713361.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLVPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            13131..13559
                     /locus_tag="VYO70_001122"
     CDS             13131..13559
                     /locus_tag="VYO70_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1713362.1"
                     /translation="MELEISDFLGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            13697..14647
                     /locus_tag="VYO70_001123"
     CDS             13697..14647
                     /locus_tag="VYO70_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1713363.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNACVFKKTKDISGVIGG"
     gene            14649..15392
                     /locus_tag="VYO70_001124"
     CDS             14649..15392
                     /locus_tag="VYO70_001124"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1713364.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLTQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     VGLGPRILRAETAPLYALSALSVLVELEK"
     gene            15403..17199
                     /gene="pepF"
                     /locus_tag="VYO70_001125"
     CDS             15403..17199
                     /gene="pepF"
                     /locus_tag="VYO70_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1713365.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYAFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQINLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(17259..17936)
                     /locus_tag="VYO70_001126"
     CDS             complement(17259..17936)
                     /locus_tag="VYO70_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713366.1"
                     /translation="MEPDIFIITKLLSDPSRMHILDILMDGKAHTVNEIASFTKIKQH
                     TVSYHLKLLTEAQVTTLQTYGRFHYYSLKSAAIAEMLEFLSFYSPQRDVKSYKQHIHK
                     KELKVARTCYDHIAGELGISITNYLLQENLLSESEKDFQLTEAGKSYFQRKLHIDTDE
                     LKKKKRKFCPKCLDWSERKNHVGGALGNALLEFFSEKQLVIPAQTPRSLTITEKGKEF
                     LEKEWGI"
     gene            18183..18953
                     /locus_tag="VYO70_001127"
     CDS             18183..18953
                     /locus_tag="VYO70_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713367.1"
                     /translation="MLYLWGKERVKKLSVKNLKKQYGSYEAVKGISFELGDGECLGIL
                     GINGAGKTSTLKMIYSLTSISSGEIDILGQNIETNRRKSKSCLGIVGQEDMLDMTLNV
                     YDNLFAHGLCYGISSVELRKRIDSLLDFVQLSKHAQKMVNQLSGGMRRRLVLARALIN
                     RPDIIILDEPTVGLDIQSRNIIWDKLLELKELGVSIIITSHYMNEIEYLTDRVLMLHQ
                     GTIKEEGTVEDLLIKYDADNLETLFWGLTGTEKEDLHV"
     gene            18946..19710
                     /locus_tag="VYO70_001128"
     CDS             18946..19710
                     /locus_tag="VYO70_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713368.1"
                     /translation="MFKLSFHSIGHVVVRNYMSFRNLFKISIVPNLIDPLFYLLAMGF
                     GVGAYLTHVNGMLYRDFVITGLIAATAMSAATAETTVNAFIQYKIEKTYDAMLMTPIN
                     TNDIVVGQAIWAGIRAVIFGGIFWLISMLITAHFHVLMLLIPLILFIVGYLFGVLGLI
                     FTYLAPSREFLNYYNVLVIRPLYMFSDTFFPITSLPPILGDLTWFSPLYHATRMIRII
                     WGGEVTGLYLHLIWLIALALLITVLPMYMLHRRYYR"
BASE COUNT         6067 a         3401 c         4305 g         6026 t
ORIGIN      
        1 tataaagaga gaaagatatt catgagagat ttattatcta aaaaaagtca taggcaatta
       61 gaattattag aattattatt tgaacataaa cgttggtttc atcgttctga actagcagag
      121 ttattaaatt gtacagaacg tgcagtcaaa gatgatctat cccatgttaa atctgctttt
      181 cctgacttga tttttcattc ttctactaat ggtatacgca ttattaatac cgatgatagt
      241 gatattgaaa tggtttacca tcatttcttt aaacattcaa ctcatttttc gattttagaa
      301 ttcatcttct ttaatgaagg ttgtcaagct gagagtattt gtaaagaatt ttatatcagt
      361 tcatcttcgc tctatcgtat tattagccaa atcaataaag tgattaaaag gcaatttcaa
      421 tttgaagtca gtctgacccc tgttcaaatc attggaaatg agagagacat tcgttacttt
      481 tttgcacaat atttttcaga aaaatattat ttcctagaat ggccatttga aaatttttca
      541 tcagagccac tatctcaatt gttagaattg gtttataagg aaacaagctt tccaatgaat
      601 ttgtcaacgc atagaatgct aaagttgctc ctagttacga acctatatag aataaagttt
      661 ggtcatttta tggaagtaga taaagattct tttaacgacc aaagtttgga ttttttaatg
      721 caggcagaag gaatagaagg tgttgctcag agttttgaat cagaatacaa tatctcttta
      781 gatgaagaag ttgtttgcca attatttgtg tcttactttc aaaaaatgtt tttcatagat
      841 gaaagtctct ttatgaaatg cgtaaaaaag gatagttatg ttgaaaaatc ttaccatcta
      901 ttgagtgatt ttattgatca gatttcagtc aagtatcaga ttgaaatgga gaataaggat
      961 aatctgattt ggcatctgca taataccgca catctgtatc gtcaagagtt gtttactgag
     1021 tttattttat ttgatcaaaa agggaataca atcaggaact ttcaaaatat ttttccaaaa
     1081 tttgtttcag atataaaaaa agagctttct cactatttag agactcttga ggtttgttct
     1141 agttcaatga tggtaaatca tctatcttat accttcatca ctcataccaa acatttggtg
     1201 attaatttgc tacaaaatca gcctaaacta aaggttttag ttatgagtaa ttttgatcag
     1261 tatcatgcaa aattcgttgc agagacactt tcttattact gtagcaataa ttttgaactg
     1321 gaagtttgga ctgaattaga attatcaaag gaatctttag aagattcacc ttatgatatc
     1381 attatttcta attttattat tcctcctatt gaaaataaga gactcatcta ttcaaataat
     1441 ataaacacgg tctcactcat atacttgtta aatgccatga tgtttattcg attagatgag
     1501 taagcaaaaa taacaaaaaa acgctatttc gcgtttgaac tgtacctcaa aagttagaca
     1561 gaaaaaatct aatttttggg gtgtttttgt taagaaaaga ccttcaatca tttaagcaaa
     1621 atgtacatct agatttatag ccctctaaaa ctcttgcttt ttttgtagaa gatacgaaaa
     1681 aagccataaa accaaggctt ttcctgtttt atgtagatgt ttacacatga aaattgaagt
     1741 attttcaaaa gctaattgga gtgattttat tcatgatggg ataaatgatc tggccaaaaa
     1801 tgattgagaa atagttgctt aaaaagcatt tgcatgatac tatatattga aaggaggtga
     1861 taatatggta gaacaaagaa aatcaattac catgaaagat gttgctttag aagcaggagt
     1921 tagtgttgga actgtttcac gtgtaattaa taaagaaaaa ggcattaaag aagtaacttt
     1981 gaaaaaagtg gaacaagcga ttaaaacttt gaattacatt ccagattact acgctagagg
     2041 aatgaaaaaa aatcgaacag aaacgattgc aatcattgta ccaagtatct ggcatccctt
     2101 cttttcagaa tttgctatgc atgtggaaaa tgaagtctat aagagaaata acaaattact
     2161 cttatgttct atcaatggta caaatagaga gcaagactat ctggagatgt tgcgtcataa
     2221 taaagttgat ggagtggttg ccattaccta tagtccaatt gaacattact tgacgtcagg
     2281 aattcccttt gttagtattg accgcacata ctcagatatt gccattcctt gtgtttcatc
     2341 agataatgat gcaggtggaa gagaagcagc taaacaatta attagtaaag gatgtcagca
     2401 tttagccttt gtgggcggac ataatacaac cattaatgaa accaaacgtc gtagaatttc
     2461 atttgaaaag tatgtccaag agcataaaat acctcttagt atttttgatt tggatgagac
     2521 agttactgac tattatggaa agctagagag tttcttggaa gaaaattctt ctatagatgg
     2581 aatttttaca atcaacgatt ttacagcttt ggatgtgatt gaaatactaa aaaaaaatgg
     2641 caaacgtatt cctcatgatg tccaaattat tggatacgat ggaattaaaa tggctggaga
     2701 tagagattat ctactttcaa ctattgaaca accattggaa gaaatggcaa aagaagcggt
     2761 tcgtattttg tttgatataa ttgatggaaa gactgttaat ttgcagacaa tcctaccagt
     2821 aaaatttgtt gaaggaaaaa caacaaaaaa tgaaaataag tcttgacaga aagcgctatc
     2881 aatgatagaa tgaattcaga taaaaagatt tatttttaaa acaaaaatga aacgtttcaa
     2941 aaaagaaata aaaagacagc gccaagcgct atcttttcta gaaaaaaatg aaacgtttca
     3001 aaaaaggagg ttgctatgaa tagcaaagcg aagcaagttt ctctttggga aagaatcaag
     3061 aaacaaaaac tcttgttatt gatgactgtc cccggtttag ttttaacctt tatctttaaa
     3121 tacatcccta tgtatggggt tttaatcgca tttaaagatt acaatccttt aaaaggaatt
     3181 ttagggagtg attggattgg tttttctgag tttacaaaat tcatatcctc tcccaacttt
     3241 ggtatcttgt tagccaacac attaaaatta agtatctatg gtttattgct tggcttttta
     3301 ccaccaatca ttcttgcgat tatgctcaat caactcttga gtgaaaaagt caaaaaacga
     3361 attcagctca ttttatacgc accaaacttt atctcagtcg ttgttattgt cggtatgatt
     3421 ttcctcttct tttcagtggg aggaccaatc aacaattttc tttctatgtt tggaatgaag
     3481 gctgacttct tgacaaatcc agacttcttt agacccttat acatctttag tggtatctgg
     3541 caaggaatgg gctgggcttc aacgctctac acggcaacat tggtaaatgt agatccagcc
     3601 ttagtagaag cagctcgact ggatggagcc aatatcttcc aacgaatctg gcacattgat
     3661 attccagctc ttaagcctat tatggttatc caatttgttt tagctgcagg tggaattatg
     3721 aatgtcggat atgaaaaagc attcttgatg cagacatcgt taaatttgcc aacttctgaa
     3781 attatctcga catatgtcta taaagttggt cttgtatcag gagactattc ttactcaaca
     3841 gcggttggtt tgtttaatgc agtgattaac gtagtattgc ttgttgcagt taaccaaatc
     3901 gttaaacgca tgaataatgg tgaaggaatt taaggaggaa agtatgaaaa attcgattat
     3961 ggatacaaaa tttgatagac gtatcttact cttaaataaa atcattattg tctttatcgt
     4021 tttgatgact ttgcttcctt tactttatat cgtcgtagca tcctttatgg atcctaaggt
     4081 tctggttagt agagggatta gctttaatcc agccgattgg actgtagaag gttaccagcg
     4141 tgtattcagt gaccaatcta ttctaagagg ttttatcaat tctctactat actcttttgg
     4201 atttgcagct ttaacagtct tgctatctgt gtttacagct tatcctcttt ccaaaaaaga
     4261 tttggtaggg cgccgttgga ttaactactt cttgattgtg actatgttct ttggtggtgg
     4321 tttagtccca acttacttgc tcgtaaaaga attgggaatg ctcaatactc catgggctat
     4381 cattgttcca ggtgctgtca acgtttggaa tattattctt gctagggcct atttccaagg
     4441 attgcctgaa gaattagttg aagctgctgt cattgatggt gcaaatgatt tacagatttt
     4501 cttcaaaatc atgcttcctc ttgcaaaacc aattatgttt gttctcttcc tttatgcttt
     4561 tgtaggacag tggaactcat actttgatgc aatgatttat atcaaggatc caaacttgga
     4621 accattgcaa cttgtacttc gtaaaattct cattcagagc caaccaggtc aagacatgat
     4681 tggagcacaa gcggctatga atgaaatgaa acgtttagct gaattgatta aatacgcaac
     4741 tattgtcatt tccagcttgc cattgattgt tatgtatcca ttcttccaaa aatactttga
     4801 taaaggaatt atggctggtt cacttaaagg ataaaaaaag aaaaaataaa aggagttttc
     4861 tcatgaaatt caaaacattc tcaaaatcag cagttttgtt gacagctagt ttagcagtac
     4921 ttgcagcctg tggctcaaaa aatacagctt caagtccaga ttataagttg gaaggtgtaa
     4981 cattcccgct tcaagaaaag aaaacattga agtttatgac agccagttca ccgttatctc
     5041 ctaaagaccc aaatgaaaag ttaattttgc aacgtttgga gaaggaaact ggcgttcata
     5101 ttgactggac caactaccaa tccgactttg cagaaaaacg taacttggat atttctagtg
     5161 gtgatttacc agatgctatc cacaacgacg gagcttcaga tgtggacttg atgaactggg
     5221 ctaaaaaagg tgttattatt ccagttgaag atttgattga taaatacatg ccaaatctta
     5281 agaaaatttt ggatgagaaa ccagagtaca aggccttgat gacagcacct gatgggcaca
     5341 tttactcatt cccatggatt gaagagcttg gagatggtaa agagtctatt cacagcgtca
     5401 acgatatggc ttggattaac aaagattggc ttaagaaact tggtcttgaa atgccaaaaa
     5461 ctactgatga tttgattaaa gtcctagaag ctttcaaaaa cggggatcca aatggaaatg
     5521 gagaggctga tgaaattcca ttttcattta ttagtggtaa cggaaacgaa gattttaaat
     5581 tcctatttgc tgcatttggt ataggggata acgatgatca tttagtagta ggaaatgatg
     5641 gcaaagttga cttcacagca gataacgata actataaaga aggtgtcaaa tttatccgtc
     5701 aattgcaaga aaaaggcttg attgataaag aagctttcga acatgattgg aatagttaca
     5761 ttgctaaagg tcatgatcag aaatttggtg tttactttac atgggataag aataatgtta
     5821 ctggaagtaa cgaaagttat gatgttttac cagtacttgc tggaccaagt ggtcaaaaac
     5881 acgtagctcg tacaaacggt atgggatttg cacgtgacaa gatggttatt accagtgtaa
     5941 acaaaaacct agaattgaca gctaaatgga ttgatgcaca atacgctcca ctccaatctg
     6001 tgcaaaataa ctggggaact tacggagatg acaaacaaca aaacatcttt gaattggatc
     6061 aagcgtcaaa tagtctaaaa cacttaccac taaacggaac tgcaccagca gaacttcgtc
     6121 aaaagactga agtaggagga ccactagcta tcctagattc atactatggt aaagtaacaa
     6181 ccatgcctga tgatgccaaa tggcgtttgg atcttatcaa agaatattat gttccttaca
     6241 tgagcaatga caataactat ccaagagtct ttatgacaca ggaagatttg gacaaaattg
     6301 cccatatcga agcagatatg aatgactata tctaccgtaa acgtgctgaa tggattgtaa
     6361 atggcaatat tgatactgag tgggatgatt acaagaaaga acttgaaaaa tacggacttt
     6421 ctgattacct cgctattaaa caaaaatact acgaccaata ccaagcaaac aaaaactaga
     6481 ggttgattat gggagataag aaatacacag tagaaaaagc caatcgtttt atagcagaaa
     6541 ataaacatct cgttaatact caatataagc ctgaagaaca tttttcagct gagattggtt
     6601 ggatcaatga tccaaatgga tttgtctatt ttcgtggaga ataccatctc ttttatcaat
     6661 tctatccata tgatagtgtt tggggaccta tgcactgggg acatgctaaa agtaaggact
     6721 tggtgacttg ggagcacttg ccagtggcac ttgctcctga ccaagattat gaccgaaatg
     6781 gttgtttctc aggctctgcc attgtcaagg atgatcgcct ctggctcatg tacactgggc
     6841 atatcgaaga aaaaaccggt gtccgccaag tgcaaaatat ggcattttca gacgacggga
     6901 ttcactttga aaagattgaa caaaatccag ttgcaactgg agcagacttg ccagatgagt
     6961 tgattgctgc tgatttccgt gatccaaaac tctttgaaaa agatggacgc tattactccg
     7021 tagtagctgc caaacacaag aataatgtgg gctgtatcgt tctactaggg tccgataacc
     7081 tagtagaatg gcagttcgaa tccatctttt taaaaggggt agaacaccaa ggctttatgt
     7141 gggaatgccc agattacttc gagttagatg ggaaagattg ccttattatg tcacccatgc
     7201 gttatcagcg tgagggagac tcatatcata acatcaactc atcgcttttg ttcacgggta
     7261 aggtagattg gggagaaaaa tgttttatcc cagaatcagt tcaagaaatt gatcatggcc
     7321 aagacttcta tgcgcctcaa acattgttgg acaatcaaaa tcgtcgtatc ctgattgctt
     7381 ggatgcagac atgggggcgt acccttccaa cccatgacca agaacacaaa tgggcatgtg
     7441 ccatgacttt acctagaatt ctaagattgg aagatggcaa actaagacaa ttccctgtta
     7501 aaaaaggcca atatcaaatc caaatagata aagattgtca ttaccactta ggaaatgata
     7561 tagattatct tgaatttggt tatgacagta atgcgcagca agtttacatt gatcgtagcc
     7621 atcttattca aaaaattcta ggtgaagaag aacaggacac tagtcgacgg tatgtagata
     7681 ttgaagctaa agaattggaa gttgttctag ataaaaattc catcgagatt tttgtcaatc
     7741 aaggtgaagc aagcttgact gcaacttatt acttaacggt gccagctgag ctatcacgaa
     7801 ttgattaaaa attaagttat ttctcctaaa gaaaaagttc tctttctaaa atagtggaaa
     7861 gaggactttt tgtgttttgg gtatataagc ttagtttatg gtatttgtaa aattggtgtt
     7921 ggattatgat ttaaactagt tttctaaaga atttgaaaaa aattttattt aagcaaaaaa
     7981 aaccttggtt ccaagacttt tcctgttgta tttagatgcc ccctacaggg atcgaacctg
     8041 tgacccacgg attaagagtc cgctgctctg ccagctgagc taaggaggca aagaaaaaag
     8101 ctgtattggt gccgaaactt cacggtttgt attgaacccg cgcaattaag caggtgggca
     8161 actcgctcta actgaagctg tttccgtgtg agacggccta catgctgtta gaagactttt
     8221 gtttccctaa taatacaaaa aatagtcggt caacacttaa gtgtgaagtc gtacaccaca
     8281 gcgtttctat acttatatga taccactttt tcaaaaaaaa tcaagaggaa agtgcaattt
     8341 tttgaaaagg atttcagatt tttcgcaaac gatcgatagc ttcctttctt tgagccaaag
     8401 cccgttcata tttaccagta tcttctgctt ggaaatagtg atgattacga attttttctg
     8461 gcagatagtc ttgcttgatc caatttccag gatagttgtg tggatagaga tagtcttggg
     8521 cattccccag ttccttgctt ccactgtagt gcccatcacg caggtgtcgc ggaataggca
     8581 agtgccctga tgttttgagg tcagcaagtg ccttatccat agctacatag gctgagttgg
     8641 attttggaga aagggccaaa tcaatcacga cattggcaat gagaatgcgg gcttctggga
     8701 acccaatctt ctgggcagca tccagagcag tcacggtatg aatctgggcc tcaggattgg
     8761 ctaagccgat atcttcatag gcgataacag tcaagcgacg agcgagacta ggcagatccc
     8821 cagcctcaat caagcgggca gtatagtgga gactggcatc cacatctgag ccacgaatag
     8881 acttttgcag ggcagataga acatcatagt gaccgtctcc atccttgtcc atagtgatgt
     8941 agcttctctg aagactattt tccatgatgt ctaaggtgat gtggcgaatg ccctcgtcat
     9001 tctcaggggt agagagaaca gccaagtcca gtgagttaaa ggcagagcga aggtctccgt
     9061 ttgtagaggt tgcgatgaaa tccagcgcat cctcatctag ttctattgga aaatcaaaac
     9121 cacgttcagg gttacttaga gctatctgca gggcctcttt gacgtcttgg ttagacagag
     9181 gttccaactc gaaaatttga acacggctac gaatggcagg agtgacagag aagaaaggat
     9241 tttcagtcgt tgctccaatc atgatgacca gtccactttc caagagaggc aagaggaagt
     9301 cttgcttggt cttatctagt cgatgaattt cgtctagcaa taggacgaga ccaccagaaa
     9361 atttagcttc ttccgagatt tcttgcagtc gctttttact atcaactgtc gcattgaaag
     9421 ttcgaaaggc atacttggtc gttccagcga tggcagaggc aatactggtt ttgccgattc
     9481 ccggagggcc atatagaatc atggaggaca ggcggttggc ttccaccatg cggcggatga
     9541 tttttccagg tccgaccaga tgctcctgac cgatgacctg gtcgatggtt ttagggcgca
     9601 tgcgaagcgc gagattgtct ggcatagcag tcctttctaa cgtggatttg agtagaatga
     9661 ctcatctact atatcttact atgtccattc tagcctatta gttgtgatat ttcaatggtt
     9721 ttgtaataat cagggagtga gatagaatct tttttactat ttaatttaat gaggagaggt
     9781 atcagcatga gagatttgat tttccttaaa gctcaaaata ggatagttga gcctaaagaa
     9841 gtggaagtgc tgacttgaga aataatattg ctgaatgtca ttgtatgaac tataatgaaa
     9901 agagaggtga taagcaatgg aaatgatgga ccaagtcagg aaattttgat ttttacaaaa
     9961 caaattcgtc attggattct tagtgatcaa gttatttcag ggaaaagaaa acttttcttc
    10021 cgggaagata ctccaaaaga gattttagat ttgtacgaaa acattaaatc taaacttgat
    10081 tttgcttacc aagaagttca ttctaataat gggttgaaaa aatatgaaaa atagaataat
    10141 tgatgttttg aagtagtaaa tcgcctcctt gtactaactg ttgaaaatcc tgatttcgaa
    10201 gacttgagag ttaatcatac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag
    10261 gctgtacttg agtacggtaa ggcgacgttg acgtggtttg aatttgattt tcgaagagta
    10321 tcagtttgta aaaattggag ataaaaaata tagagtacgc agtgttccta tgattcattc
    10381 aactccgcct caatctgtct tagatcgaga tacttttaca attgactata cagatgatga
    10441 attgctcgat aaagaagcag tttttactac tcattagaaa aataggtctg aataaattgg
    10501 cttcccttag aaccacatct aggggctttt atgtataata gaataataag tatcactcct
    10561 ttacaatacg tgtaacacgt gttataatgg tgttgtcagg aggtaaaagc gctatgccta
    10621 tgacacaaaa agagatggtt aaactcctta cggcccatgg ttggataaag actagaggcg
    10681 gtaaaggctc ccatattaaa atggagaagc aaggggaaag acttatcaca atccctcatg
    10741 gtgagctgaa taagtacact gaaagaggga taagaaagca agctgggttg taaaattcag
    10801 cccctgtttt ttgatatgga ggtagtgaaa tgttagttac gtatccagct ttattttatt
    10861 atgacgacac agacggaaca gaagcgactt attttgtcca tttcccagat tttgaatact
    10921 cagctacaca aggagagggg atttctgagg ctttggctat ggggtcggag tggctaggga
    10981 taactgttgc agatttgata gagagtgatg gagagttacc tcagccatca gatatcaata
    11041 gcttgtcttt aattgataat gatcctttta aagatgatga agatttcgtg tcaacctatg
    11101 accttgataa atcttttatt tctatggtat ctgttgatgt atcagaatac ctaggaagtc
    11161 aggaacccat taaaaagact ttgaccatac caaaatgggc agataagttg ggacgagaaa
    11221 tgggacttaa cttttctcag actttgacag acgctattgc agataagaaa gttcaagcct
    11281 aagattaagc gtggtatcgt ggtaatagtc aataaaaaag cacgtccact tgtgctagtt
    11341 actcgcctat tgattttata gatttattag ccctttcgag ggctttttat attgattttt
    11401 atgagaaatg aagaaatcag acttttaaga aaatcactga taccaagggt ttaaatgagg
    11461 taatatggta taattaggac ataaaataat tttgtggtaa gatggtagta tctattttag
    11521 catatttccg agcaatcggg gcgattaaag agtcgcatag aaagaggaca aaatggcaac
    11581 atacggattt ttagatattt tagaggaaga gttggacaag aactttccct ttgactttga
    11641 gattagttgg gacaaacgca accatgcggt agaagtgagt tttctattgg aagcgcaaaa
    11701 tgctgcaggt gtggagatgg tggatgaaga cggagaggtt tcatcagatg acattctttt
    11761 tgaggaagcg gtccttttct acaatcctgc caagtcaaca gtcaatgagg aagactattt
    11821 gactgttatt ccttacctgc ctaaaaaagg attttcccgt gaatttttag cttattttgc
    11881 gcttttcctt aaagatactg ctgaggttgg gctagatgtc ctcatggact ttttggaaga
    11941 cccagaagca gaggaatttg tcatggaatg gaaccaagaa gtctttgaag aaggaaaagt
    12001 tggcttggaa gagggagaat tttaccctta tccaagatac taggagttgg ttggaggtgt
    12061 tatgaagaaa attgggactt atttggttta tgtgctagct gttgtcttta ttatgctagc
    12121 ttttgcttgt ggaacaatcg catttgcaga gttggggtat tccgcagttt tagcctttac
    12181 ttttggttat gccttcgctc ttctaagcat gtatttaatc tttattcttc atgagctggg
    12241 tcatgctttt tgtggctact tgacaggcta tcggctggtg gcttttggat taggacattt
    12301 tattttgacc aaaaagtcag gcaggtttca tcttagtaga acagccattc tgaaaaatgt
    12361 tggtgctcaa tacattggtt taaaagagga tgaaagcgat caaagaatca tcctgatgct
    12421 ttcaggaggc ttgatggttc atctcggctt gatattattg gcgatagtgt ttggattttt
    12481 gacaagaagc tggtattttg cagggacttg gatttttctt aatttatctt tcttcctaaa
    12541 taacattttg ccagtcgaca tcaccgatgg agcaaaaatt tgggaattac tacaacaccc
    12601 tgaaaatacc aaatacgcct acctggtatt gagacattct gctcaaactt tgctggttcc
    12661 tcaagaatat gatttgaaag actttgttca agcagttcct gaggatgcac gagggagttt
    12721 tgcggatggt gtattaggta tgcaaggtga agtttccatt ttggaaggga aggaagaagt
    12781 agctaaacag cagtttcaag cattacttga gaggacaact actccgatga tgcagacagt
    12841 tgcccaacta tccctgcttc atatcgcctt gttggaagga gattttgagc aagcggagca
    12901 atatgctagt attcgacagg tcaaatcgtt tctgtctctc aaactgtcca atcttcaagt
    12961 gatacaagcc tggtatcaat taaaggtgaa gaaagatgta gatcgaactc gtaaggctct
    13021 caagattgct agacagaaaa tggatagtag tcgaatgttg agggacgaga aacgctatta
    13081 tgaaaacttg ttagcagagc tggaaaagga aatgttagaa ggaactgaag atggaactag
    13141 aaatttctga tttcctaggc tgtaagattg ctttgttttg tggggataag cttttgacta
    13201 tcttacgcga tgataaggca agcattccct gggccaatat gtgggaactg ccaggtggtg
    13261 gacgtgaagg tgatgaaagt ccttttgagt gtgtagcgcg tgaagtttat gaagaactgg
    13321 gcattcatct gactgaagat tgtctgcttt ggagtaagat ttacccaagt atgctttttg
    13381 cagacaaaca gtccgttttt ctagttggtc agttgacaca gaaccagttt gacagtatcg
    13441 tatttggaga tgaaggacag ggctatcagc tgatgaatgt tgaggaattt cttagttcca
    13501 gtcaagttgt gcctcagttg caagagagat taaaagatta tttaaaagta agtgattaga
    13561 aaggatggtt cggttaaatt tctaaactga acccgcccta aacacggtgc caaaaagata
    13621 aacttctctt agacacaaac gtcttcagag aatttcctat tttggttttg tgttttacgg
    13681 gcttggtatc ttaataatgg aaacatggca agagttaaaa gttacagtga agcgtgaggg
    13741 agaggagttg gtttccaatc tcttgattga gctaggagcg caaggagtcg caatcgaaga
    13801 cagtatggac tatatgggaa atgtggatcg ttttggcgag attttcccag aggtggagca
    13861 gcaagaagaa atcgtagtga cagcctacta ccctgacacg gtggatgtga cagtggttga
    13921 ggcggacttg caggctcgct tagcagaatt gacggatttt atggatttgg gtgaagtcaa
    13981 gatggggacg actgccttgg ctgaggaaga ctgggcagac aactggaaga aatactatga
    14041 gccagctcgt atcactcatg atttgaccat cgttccctct tggacagact atgaggcgac
    14101 tgcgggagaa aaaattatca agctagatcc tgggatggct tttggtactg ggacccatcc
    14161 aactaccaag atgagcctct ttgccttgga acaggttctt cgtggtggcg agacagtgct
    14221 agatgtgggg actggttcag gggttctctc cattgctagc tcgctacttg gtgccaagga
    14281 aatcttcgcc tatgacctag atgatgtagc agttcgtgtt gctcaggaaa atattgaact
    14341 taaccctgga atggaaaata tccacgttgc tgctggagac ttgcttaagg gtgttgagat
    14401 tgaggcagat gtgattgtag ctaatatctt ggcggatatt ctcattcatc tgacagacga
    14461 tgcctatcgt ttggtcaagg atgaaggcta cctgatcatg agtggcatta tcaaggacaa
    14521 gtgggacatg gtgcgcgagt cggctgagtc agctggattt ttccttgaaa cccacatggt
    14581 tcaaggggaa tggaatgcct gtgtctttaa gaaaaccaag gatatttctg gtgtgattgg
    14641 aggctagcat gcagcagtat tttgtaaaag gcagtgctat ctctcctgtc actatcgagg
    14701 acaaggaaac cagtaagcat atgtttcagg ttatgcgctt gaaagaagag gatgaggtta
    14761 ccttagtctt tgatgatggc atcaagcgct tggcgcgcgt gctggatatg gaaaatcgtc
    14821 agtttgagtt ggtccaagaa ttaaatgaca atgtggaact accagttcaa gtgaccatcg
    14881 catctggatt tcccaaggga gacaagctgg agttcatcgc tcaaaaagta accgaactgg
    14941 gtgctagcca aatctgggcc tttcctgcag actggtcagt tgccaagtgg gatggcaaga
    15001 aattgggtaa aaaagttgaa aaactagaaa aaattgccct tggagcagcc gagcaaagca
    15061 agcgtaatat tgtaccaagt attaaacttt tcgagaaaaa agcagatttt ctaactcagc
    15121 tggaccagtt tgactctatc atagtagcct atgaagaatc agctaaagaa ggagaagccg
    15181 ctgcgcttct gcaagcagtc actggtcttg aaacaggagc caaactgctc tttatctttg
    15241 gtccagaagg cggtctctca cctgcagaaa tcgaaagttt tgaagctaag ggagccgttt
    15301 tggtaggcct aggtcctcgt attttgcgag cagaaacagc accgctttac gccttatcag
    15361 cccttagtgt tttagtagaa ttagagaaat aagaggaaga aaatggaaca aaaacaccgt
    15421 tcagaatttc cagagaagga actttgggat ttaacagctc tataccaaga ccgtgaggat
    15481 ttcttgcgtg caatcgagaa agctcgcgaa gacatcaacc agtttagccg tgattacaag
    15541 ggcaatcttc acacttttga ggatttcgag aaggcctttg cggaattgga acagatctac
    15601 attcagatga gccatattgg caactatgct tttatgcctc agacgacgga ctatagcaat
    15661 gacgaatttg caaatattgc tcaagctggg atggaatttg aaacagatgc cagcgtagcc
    15721 ttgaccttct ttgacgatgc cttggtggta gcagacgagg aagtcttgga ccgtttgggt
    15781 gaattgcctc acttgacggc agctattcgt caggccaaaa tcaaaaaagc ccactatcta
    15841 ggggctgatg tggagaaggc cttgaccaat ctcggtgaag ttttctatag tccgcaggac
    15901 atttatacta agatgcgagc tggggatttt gaaatggctg actttgaagc ccatggcaag
    15961 acctataaaa acagctttgt cacctatgag aatttctacc aaaatcacga ggatgctgag
    16021 gttcgtgaga aatccttccg ttccttctca gagggacttc gtaagcacca aaatacggct
    16081 gcagcagcct atctggctca ggtcaagtct gaaaaactct tggctgatat gaagggatac
    16141 gactctgtct ttgattatct tctagctgaa caagaagtgg accgtgtcat gtttgaccgc
    16201 cagattaacc tcatcatgaa ggactttgca ccagtcgctc agagatacct caagcatgtt
    16261 gccaaggtaa atggtcttga aaagatgacc tttgcagact ggaaattgga cttggacagc
    16321 gccctcaatc ctgaagtgac tattgacgat gcctatgatt tggtcatgaa gtcggtagaa
    16381 cctttggggc aagaatattg tcaggaagtt gctcgttacc aagaagagcg ctgggtggac
    16441 tttgctgcta acagtggcaa ggattccggt ggttatgcgg cggacccata tcgcgtacac
    16501 ccttatgtac tcatgagctg gacaggccgt ttgagcgatg tctatacctt gattcatgaa
    16561 atcgggcatt ctggtcaatt catcttttca gacaatcacc aaagctattt caacgcccac
    16621 atgtcgacct attatgttga agcaccgtcg accttcaatg aattgctact cagtgattac
    16681 ttagagaacc agtctaatga cccacgtcaa aaacgcttcg ctctggctca tcgcttgaca
    16741 gacacctact tccataattt catcacccac ctcttggaag ccgccttcca gcgtaaggtg
    16801 tatacattga ttgaagaagg agaaaccttc ggagcaagca aactcaacag cattatgaag
    16861 gaagttttga cagatttttg gggcgatgcc attgaaatcg acgacgatgc agctttgact
    16921 tggatgcgcc aagctcacta ctatatgggc ttgtatagtt acacttactc tgctggtctt
    16981 gtcatctcga cagctggtta ccttcatctt aaaaactcag aaactggagc tgaagactgg
    17041 ctcaatctcc tcaaatcagg tggtagcaag acaccacttg agtcagccat gattatcggt
    17101 gctgatattt caacagacaa accactccgt gataccatcc aattcttgtc tgacacagtt
    17161 gaccagatta tcgcctacag tgctgagttg ggggagtaag gggatagaga ggttgttata
    17221 gaatgggtat ggacagttat ttgcttaaaa gtccatgttt aaatccccca ttccttttcg
    17281 aggaattctt tgcccttctc tgtaatggta agtgaacgtg gcgtttgtgc agggatgacc
    17341 aattgttttt cactgaaaaa ttctaaaaga gcattgccga gagctccgcc gacatgattt
    17401 ttacgttcgc tccaatccaa acatttaggg caaaatttcc gttttttctt tttcaattca
    17461 tccgtatcaa tgtgtaattt tctttggaaa taagacttcc cagcttcagt gagttggaag
    17521 tctttttctg attcgctgag gagattttct tgcaagagat aattggtaat ggaaatgcca
    17581 agctcaccag caatatggtc atagcaggtg cgagcgacct ttaattcctt tttgtgaatg
    17641 tgttgtttat aacttttgac atcacgctga ggagagtaga agctgagaaa ttccagcatt
    17701 tctgcaatgg cagcactttt taaagaataa taatgaaaac gtccataggt ttgtagagtg
    17761 gttacctgcg cttcggtaag taatttgagg tgatagctga cagtatgttg cttgattttg
    17821 gtaaagctgg caatttcgtt aaccgtgtga gcttttccat ccattagaat atctaaaatg
    17881 tgcatccgag aagggtctga taagagtttt gtgatgatga aaatatctgg ttccataaaa
    17941 attttctcca aatttcatat atcgatgatt ttctatcttt tattatacta taattaaagc
    18001 acaagaaaaa tagattttaa aaatgtttta ctagagaata ttttaaattt tattgtctga
    18061 gtttaaatgg aggtaaaatt acaaaagtaa ttgcagcagt atccacagtt gaaggacgtt
    18121 aatcttaaga ttacattctc aacatttttt caaaagtgag atgaattaac aaagtatcca
    18181 acatgctcta tttgtggggg aaagaaagag tgaagaagtt aagtgttaaa aatctaaaaa
    18241 agcaatatgg ttcttatgaa gctgtcaagg gaattagctt tgaattggga gacggagagt
    18301 gtttgggtat cttgggaatc aacggtgctg gcaaaacatc cactttaaag atgatttact
    18361 cactgacctc aatttcctca ggagagattg atatcttggg gcaaaatatt gaaaccaatc
    18421 gtcgtaagag taaatcttgt ctgggtattg tagggcagga ggatatgctg gacatgacct
    18481 tgaatgttta tgataatctt tttgctcacg gtctatgtta tggtatctca tctgtcgaac
    18541 ttaggaagag aatcgattct ctcttggatt ttgttcaact gagcaaacat gcgcaaaaga
    18601 tggtgaatca gctgtcgggt gggatgcgac gacgtcttgt cttggcaaga gctctcatca
    18661 atcggcctga tattatcatt ttagatgaac cgacagttgg tttagacatt caatcccgca
    18721 atattatttg ggataaactt ttggaattga aggaattagg tgtttctatc attattactt
    18781 cccactatat gaatgagatt gaatatttga cagatagggt gctgatgctc caccaaggga
    18841 ctatcaaaga agaaggaact gtagaagact tgctcatcaa gtatgatgct gacaaccttg
    18901 agacgctttt ttgggggttg actgggacag agaaggagga cttgcatgtt taagttatca
    18961 tttcactcca tcggacacgt tgtggtacgc aactacatga gttttagaaa tttatttaaa
    19021 atttcaattg tccccaacct tatcgacccc ctcttttatc ttttagcaat gggatttgga
    19081 gttggtgctt atttgacgca tgttaacggt atgctctacc gtgattttgt cattacaggt
    19141 ttaattgctg cgactgccat gtctgcggcc accgcagaaa caacggtcaa tgcctttatc
    19201 cagtataaga ttgaaaagac ttatgatgct atgttgatga cgccaatcaa tacgaatgat
    19261 attgtggtag gtcaggctat ctgggcaggt attcgtgcag tgattttcgg tgggattttt
    19321 tggctgattt ccatgcttat cacagcgcat tttcatgtct tgatgttatt gattcctctg
    19381 attttattta tagtgggtta tttgtttggt gttttgggac taatctttac ctacttggcg
    19441 ccgtctaggg aatttctgaa ttactacaat gttttggtaa tccgtcctct ttatatgttt
    19501 tcagatacct tctttcccat tacttccttg cccccaatac ttggtgattt gacttggttt
    19561 tctcccttgt atcacgctac ccgcatgatt cgtattatat ggggaggtga ggtgacaggt
    19621 ttgtatcttc atcttatctg gttgatagcc ttggcgctct tgataacggt gcttcctatg
    19681 tatatgttac atcgtagata ttatagatag tctaattatt tgtgatgttg tagtcatatt
    19741 ctaatttata ctaaacgtta ttcgaatttg gacaggtatg ggaactgtgc ctatcaata
//
LOCUS       DAVLYV010000032        19423 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLYV010000032 DAVLYV010000000
VERSION     DAVLYV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.032"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            652..1929
                     /gene="ltrA"
                     /locus_tag="VYO70_001129"
                     /pseudo
     CDS             652..1929
                     /gene="ltrA"
                     /locus_tag="VYO70_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2385..3563
                     /locus_tag="VYO70_001130"
     CDS             2385..3563
                     /locus_tag="VYO70_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1713369.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            3877..4008
                     /locus_tag="VYO70_001131"
     CDS             3877..4008
                     /locus_tag="VYO70_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1713370.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            4029..5579
                     /gene="dltA"
                     /locus_tag="VYO70_001132"
     CDS             4029..5579
                     /gene="dltA"
                     /locus_tag="VYO70_001132"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1713371.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            5576..6820
                     /gene="dltB"
                     /locus_tag="VYO70_001133"
     CDS             5576..6820
                     /gene="dltB"
                     /locus_tag="VYO70_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1713372.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIVIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVIT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            6834..7073
                     /gene="dltC"
                     /locus_tag="VYO70_001134"
     CDS             6834..7073
                     /gene="dltC"
                     /locus_tag="VYO70_001134"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1713373.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            7066..8334
                     /gene="dltD"
                     /locus_tag="VYO70_001135"
     CDS             7066..8334
                     /gene="dltD"
                     /locus_tag="VYO70_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1713374.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            8489..8929
                     /gene="adcR"
                     /locus_tag="VYO70_001136"
     CDS             8489..8929
                     /gene="adcR"
                     /locus_tag="VYO70_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1713375.1"
                     /translation="MRQLAKDIDAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            8929..9633
                     /locus_tag="VYO70_001137"
     CDS             8929..9633
                     /locus_tag="VYO70_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1713376.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            9626..10432
                     /locus_tag="VYO70_001138"
     CDS             9626..10432
                     /locus_tag="VYO70_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1713377.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGRSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            10442..11947
                     /gene="adcA"
                     /locus_tag="VYO70_001139"
     CDS             10442..11947
                     /gene="adcA"
                     /locus_tag="VYO70_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1713378.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVPNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(12769..13542)
                     /locus_tag="VYO70_001140"
     CDS             complement(12769..13542)
                     /locus_tag="VYO70_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1713379.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTVPDQKLLDAVELFDGEWVSDIQ"
     gene            13718..15121
                     /locus_tag="VYO70_001141"
     CDS             13718..15121
                     /locus_tag="VYO70_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1713380.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            15236..15874
                     /locus_tag="VYO70_001142"
     CDS             15236..15874
                     /locus_tag="VYO70_001142"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1713381.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            15886..16326
                     /locus_tag="VYO70_001143"
     CDS             15886..16326
                     /locus_tag="VYO70_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1713382.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            16319..16750
                     /locus_tag="VYO70_001144"
     CDS             16319..16750
                     /locus_tag="VYO70_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1713383.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEEDLCL"
     gene            16741..17211
                     /locus_tag="VYO70_001145"
     CDS             16741..17211
                     /locus_tag="VYO70_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1713384.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            17236..18009
                     /locus_tag="VYO70_001146"
     CDS             17236..18009
                     /locus_tag="VYO70_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1713385.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            18006..18806
                     /locus_tag="VYO70_001147"
     CDS             18006..18806
                     /locus_tag="VYO70_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1713386.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            18865..>19423
                     /locus_tag="VYO70_001148"
     CDS             18865..>19423
                     /locus_tag="VYO70_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1713387.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSST"
BASE COUNT         5940 a         3279 c         4165 g         6039 t
ORIGIN      
        1 aagtcctcgg tgggcacctg ctaccggtga acccaatagc gattcccaag cctgactatc
       61 gtgaggtagc agggagagga agggatagcg aaatcgtggc tctacgaaca ggaacgtgat
      121 agtaaggcgt atatagcgga taaggaggct tcaaactcta aagtccaaaa aggtagtcgt
      181 aacctatatg cgtaaatcac gagagtaatt gaattcggac taaggtttgt gtgaaaagat
      241 aaatctttct agagtctaaa gactctgcgt cagatttcct attttcactg taacctttta
      301 acgtcctcat atcttgtata aacgaggaaa gatgtacgac ttatcccgtg aggtctcatg
      361 agtgctgaaa gcgtagtaac aacgaatcat gagaagtcag ccgaacccat agtagtgagg
      421 aaacttccgt aatggaagtg gagcgaaggg gtgaatactc aaacagtctg gggagagact
      481 gtttgaggtc tgtcgctaga aagagaaaac gacagattga agtaatccta cttcacttgt
      541 gtctgtaaaa cgagaggtct gatagaactg gagtctgtca cgtattgact agatgaaggc
      601 tcaccaatat aagatgtccc tcaggcacca aaacaagaaa ggaatacgca catgtcaaaa
      661 ttgctagata agatattatc acgcgaaaat atgctggaag cctacaatca agtaaaatcc
      721 aataaaggct cagctgggat tgatggaatg actatcgaag agatggataa ttatctcaga
      781 caaaactggc gcttgactaa ggaactgata aaacagagaa aatataagcc tcaaccagtt
      841 cttagagttg agatacctaa accagacgga ggtatccgtc aactaggaat tccaacagtt
      901 atggatagaa tgattcaaca ggccattgtc taagtcatga gccccatttg tgaaccccat
      961 ttctcagata cgagttatgg tttcagacca aataggtcat gtgaaaaagc catcatgaag
     1021 ctcttagaat acttaaatga cggctatgag tggatagtgg atatagacct agagaaattt
     1081 ttcgatacag ttcctcaaga tagattgatg tccttagtac ataacattat cgaagacgga
     1141 gatacggaat ccttgattcg taagtatctt cattcaggtg ttatcattaa tggtcaacgt
     1201 tataaaacac tagttggtac accacaggga ggaaatttat ctcctctctt atccaatatc
     1261 atgcttaatg aattggacaa ggaattagaa aagaggggac ttcgatttgt gcgctacgca
     1321 gatgattgtg tgattacggt cgtaagcgag gcagccgcta agcgtatgat gtattcagtc
     1381 agtcgtttta ttgagaaacg gctaggtttg aaagtaaaca tgaccaagac taagattacc
     1441 agaccaagag agttgaaata tctaggtttt gggttctgga aatcatcaga tggttggaaa
     1501 agccgtccac atcaagatag tgttcggaga tttaagttta aattgaagaa actaacacag
     1561 aggaaatgga gtatagacct aacaagacgt attgagcaac tgaatttgtc tattcgagga
     1621 tggataaact atttctcatt gggaaatatg aaaagtatag tcgccagcat agatgagcgc
     1681 ttgcgtactc gcctacgagt gattatctgg aagcaatgga agaagaaatc gagacgatta
     1741 tggggattgc ttaagttagg agttcctaaa tggatagcag ataaggtatc tggctggggc
     1801 gaccattatc aattagtatc tcagaagtcg gtacttaaac gtgctatatc aaaaccagtc
     1861 ctggaaaaac gtggactggt ttcgtgtttg gattattacc ttgaacgaca tgcgttaaaa
     1921 gttagttgaa ccgccgtatg ccgaacggca cgtacggtgg tgtgagaggg gctagagatt
     1981 atcccctact cgattaactc ccctgaaatt tattttaatt atgcaaattt cacgtatttt
     2041 tgatgctgag acgacgatcc tgggaacttt tcagatattt ttttgactat ctaaatctat
     2101 cattagaaaa gcttagagcg ccaaaggatt tgagcgtttt tctgattttt aagacttttt
     2161 ccagtttctt tttcgattga agatgtaatt attctactaa ctaactaact tcttagtact
     2221 agccaacaac gataatcata attcctccta aaattaggaa taataaaggc aatagttttt
     2281 gttttttcat gtaaaaaacc tcacttttgt tttctgctat tttatgctaa aatattaaaa
     2341 atcaaattta attccaaagt ttgtaactaa agggggagcg ctacatgtct aattcatttg
     2401 tcaagttgtt agtctctcaa ttatttgcaa atttagcaga tattttcttt agagtaacaa
     2461 tcattgctaa catatacatt atttcaaaat cagtaattgc cacatcacta gttcctatct
     2521 taataggaat atcctctttt gttgcgagtc ttttagttcc gttggttact aaaaggttag
     2581 cgctaaatag ggttttatct ttatctcaat ttggaaagac tatattattg gcgatactgg
     2641 taggaatgtt taccgtaatg caatccgtag cgcctttggt gacctatcta tttgttgttg
     2701 caatttccat actagatggt tttgcagcac ccgtttccta tgctattgtg ccacgctatg
     2761 cgaccgattt gggtaaggct aattcagcct tatcaatgac tggtgaagct gttcaattga
     2821 taggttgggg attaggtgga ctcttgtttg caacaattgg tctgttacct accacgttta
     2881 tcattttagt cttgtatatc atttctagct ttctgatgtt atttcttcct aacgctgaag
     2941 tggaggtgtt agagtcagaa actaatcttg aaattttgct caaaggttgg aagttagttg
     3001 ctagaaatcc tagattaaga ctttttgtat cagcaaattt attggaaatt ttttcaaata
     3061 cgatttgggt ttcttccatt atacttgttt ttgtaacgga gttattaaat aaaacggaaa
     3121 gttactgggg atattctaat acagcatact ctattggtat tataattagt ggcttaattg
     3181 cttttaggct atctgaaaag ttccttgctg ctaaatggga aagtattctt tttcctcttg
     3241 tagctatggc aatagtgaca ctgactattc tatattttcc aaacgcacag atgtttttat
     3301 tattttcagc tttagttggt atgttatcgc aactaaaaga agttcctgaa agtgtatttc
     3361 ttcaggaaac agtagaggaa aatcatttag ttaatgtcta ttccgttctt gaagtgattt
     3421 ctacattagc attttcagta tttgttttgc taatgagcta tattactgag agttttggta
     3481 ttagcatcag tttttggcta tcagccattt gtctgatgat agaagctatt ttaatttata
     3541 tcagacgaga ttattttaaa tgagagagtg gttaaaaagt ctttaaaaat cagaaaagcg
     3601 catagtatca ggtgttgaaa aaacttgata ttgtgtgttt tattatggga agatttactt
     3661 cattgtctcc tgaaatggag ttttgaagca ggatcactct atttgaaaat atacatagaa
     3721 aaaccgcata atccttaaaa taagctgaaa tctattggaa acatacggta aacaattgaa
     3781 aagatactat ataaagtggt acaatggtag aaaaataagc tagtaagaat cactcttatt
     3841 ttagcgaaaa attacagaaa tgaatggttt ttcttgatga aacagagaaa agaattgtac
     3901 ctctttcttg gtcggacagc cttgtatttt cttatctttc tagggctgct ttacttcttt
     3961 agctatcttg gtcagggtca aggaagcttt atctataatg aattttaact tgaaggagaa
     4021 tccctattgt gtcaaataaa ccaatagcag atatgattga aaccattgag cattttgctc
     4081 agacacagcc tagctatcct gtctataatg ttttggggca ggaacacact tatggcgatt
     4141 taaaggctga ttcggatagt ttggctgcag tcattgacca actaggcttg cctgagaagt
     4201 ctcctgtggt tgtttttggt ggccaagaat atgaaatgtt ggcaaccttt gtagcgctga
     4261 ctaagtcagg tcatgcctac attccaattg atagccattc ggccttggag cgagtttcag
     4321 ctattttaga agtagcagag ccaagcttga ttattgccat ttcagccttt cccttggagc
     4381 aggtttctac accaatgata aatctagctc aggttcaaga agcctttgcc caagggaaca
     4441 actatgaaat cacgcatcca gtcaagggag atgataatta ctacattatc tttacttctg
     4501 gtacgactgg taagcctaag ggagtgcaga tttcacatga taatctcctc agctttacaa
     4561 actggatgat tacggataag gaatttgcga caccgagtcg tccgcaaatg ctggcacagc
     4621 caccttattc ttttgacttg tctgtcatgt attgggcacc gaccttggca cttggtggta
     4681 cgcttttcac tcttccttca gtcatcactc aggactttaa gcaactcttt gcggctatct
     4741 tttcattgcc aatcgctatc tggacatcaa caccatcctt tgcagatatg gccatgttgt
     4801 ctgaatactt caacagtgag aaaatgcctg gaatcacgca tttctacttt gatggtgaag
     4861 aattgacggt caaaacagct caaaaactgc gcgagcgttt cccaaatgcc cgtatcatca
     4921 atgcttacgg cccaacagaa gcgacagtag ctctgtcagc agttgccgtt acagacgaga
     4981 tgttagcgac tctcaaacgc ctaccaatcg gctataccaa ggctgattct ccaaccttta
     5041 tcattgacga ggaaggaaat aaactgccaa atggcgaaca gggagaaatc attgtttctg
     5101 ggccagctgt ttcaaaaggt tatatgaaca atcctgaaaa aacagcagaa gccttctttg
     5161 agtttgaagg tctgccagcc tatcacacag gcgatgtggg aaccatgaca gatgagggct
     5221 tgcttctcta cggcggacgc atggacttcc agattaagtt taacggttac cgcattgagt
     5281 tagaagatgt ctctcaaaac ctcaacaagt ctcgctttat cgaatctgct gtcgcagtac
     5341 cgcgctataa caaggaccac aaggtacaaa atctattggc ttatgtcatc ttaaaagacg
     5401 gtgttcgtga gcagtttgag cgagatatcg atattaccaa ggccatcaag gaagacctga
     5461 cagacatcat gatgtcctac atgatgccat ctaaattcct ttaccgagac agtttgccac
     5521 taactccaaa tggaaagatt gacatcaaag gattgattaa cgaggtgaat aagagatgat
     5581 ggagtttttt caacagcttc ctcatttaga gccatatggc aatcctcagt attttgttta
     5641 tgtgattgct gcaaccttgc ccatctttat aggtctcttt ttcaagaaac gctttgcctg
     5701 gtatgaagtg ttggtaagtc tcttctttat tgtcaccatg ttggtgggtg gaaagaccaa
     5761 tcaactagct gccttgggta tttacctttg ctgggaaata ttgctcctgc ttttctacaa
     5821 gcattatcga aaaagcaagg atggcaagtg ggtcttctac ttagttagtt ttctgtccct
     5881 acttccgatt atctttgtca aggtgcaacc agctatcaat ggaacgcagt ctttgcttgg
     5941 gttcttggga atttcttacc ttacctttcg ttcggttgga attgtcatcg agctgagaga
     6001 tggagtgatt aaggatttta ccctctggga attcctccgt ttccttctct tcatgccaac
     6061 tttctcgagt ggtccaatcg atcgctttaa gcgatttaat gaaaattatc aggctattcc
     6121 tgagcgagat gagttgatgg atatgctgga tgaatctgtc cgctatatca tgtggggctt
     6181 tttgtataag tttatcctag ctcatgtttt aggagagacc ttactacctc ctctgaagaa
     6241 tttagccttg cagtcaggtg gcttctttaa tctctatgcc ttggcagtta tgtatacttt
     6301 tggtctggaa ctcttctttg actttgcagg ttattctatg tttgctttgg ccatctcaaa
     6361 cttgatggga atccgtagcc ctatcaactt taacaagccc tttttatcaa gggatttaaa
     6421 ggagttttgg aatcgctggc atatgagtct gtccttctgg ttccgtgact ttgtctttat
     6481 gcgaatggtg atggtgttaa ccagaaagaa agtctttaaa aatcgtaatg taacctcaag
     6541 catggcctac attgtaaata tgctgattat gggattttgg catggtgtga cctggtacta
     6601 tatcgcctat ggactctttc atggactagg cttggtcatc aatgatgcct gggttcgcaa
     6661 gaaaaaaacg ctcaataagg aacggaaaaa agcagggaag gctgccctac ctgagaatcg
     6721 ctggattcag ttgcttggca tggttatcac tttccatgtt gtcatgttgt cattcttaat
     6781 cttttctgga ttcttgaata atctatggtt taaaaaataa aaggaaataa aatatggata
     6841 tcaaatcaga agttattgaa attattgatg agttgtttat ggaagatgtt tctgacatga
     6901 tggatgaaga tctttttgat gcaggtgtct tggatagtat gggaacggtt gagttgattg
     6961 tggagattga gaaccgtttt gacattcgtg tccctgtaac agagtttggt cgtgacgact
     7021 ggaatacagc taataaaatc atagctggta ttgtggagct acaaaatgct taaacgctta
     7081 tggatgatct tcggaccggt cttgatcgct ggtttgttgg tttttctgct cattttcttt
     7141 tatcctactg agatgcatca taatctagga gctgaaaagc gttcagcagt ggctactact
     7201 atcgatagtt ttaaggagcg aagtcaaaaa gtcagagcac tatctgatcc aaatgtgcgt
     7261 tttgttccct tctttggctc tagtgaatgg cttcgttttg acggtgctca tcctgcggta
     7321 ttagctgaga aatacaatcg ttcctaccgt ccttatcttt taggacaggg gggagctgca
     7381 tcgcttaacc aatattttgg aatgcaacag atgttaccac agctggagaa taaacaagtt
     7441 gtgtatgtta tctcacctca gtggttcagt aaaaatggct atgatccagc agccttccag
     7501 cagtatttta atggagacca gttgactagt tttctgaaac atcaatctgg ggatcaggct
     7561 agtcaatatg cagcgactcg cttactgcaa cagttcccaa acgtagctat gaaggacctg
     7621 gttcagaagt tggcaagtaa agaagaattg tcgacagcag acaatgaaat gattgaatta
     7681 ttggctcgtt ttaatgaacg ccaagcctcc ttttttggtc agttttcggt tagaggctat
     7741 gttaactacg ataagcatgt agctaagtat ttaaaaatct tgccagacca gttttcttat
     7801 caagcaatag aagatgttgt caaagcagat gctgaaaaaa atacttccaa taatgagatg
     7861 ggaatggaaa attatttcta taatgagcag atcaagaagg atttgaagaa attaaaggat
     7921 tctcagaaaa gctttaccta tctcaagtcg ccagagtata atgacttgca gttggtttta
     7981 acacagtttt ctaaatctaa ggtaaacccg atttttatca ttccacctgt taataaaaaa
     8041 tggatggact atgctggtct acgagaggat atgtaccaac aaacggtgca gaagattcgc
     8101 taccagttag aaagtcaagg ttttaccaat atagcagatt tttctaagga cggcggggag
     8161 cctttcttta tgaaggacac cattcacctt ggttggttgg gttggttggc ttttgacaag
     8221 gcagttgatc ctttcctatc caatcccaca ccagctccga cttaccatct gaatgagcgc
     8281 tttttcagca aagattgggc gacttatgat ggagatgtca aagaatttca atagataata
     8341 taatatagaa gagttcaaga atgaaactag ttttcacgtt ttttcttgac tctttttttg
     8401 cttgtgatat aattaactgg taaacaaaat gtcaaagaat agtatttttc tcttaaaaaa
     8461 gagaagtcta aaaggaaagg agtcagatat gagacagcta gcaaaggata tcgatgcttt
     8521 tttgaatgag gtgattttgc aggcggaaaa tcagcatgaa atcctaatag gtcattgcac
     8581 tagcgaggtg gccctgacca atactcagga gcatatcctt atgctcttgt cagaggaatc
     8641 tttaacaaat tcagaattgg cccgtcgtct caatgtcagt caggcggcag ttaccaaggc
     8701 cattaagtct ttggtcaagg aagggatgtt ggaaacatct aaagattcta aagatgcgcg
     8761 tgtgattttt tatcagttga ctgacttggc tcgtccaatc gctgaggagc accaccatca
     8821 ccatgagcat acacttttaa cctatgaaca agtggcgact cagtttactc caaatgaaca
     8881 aaaagtgatt cagcggtttt tgactgcttt agtaggagaa atcaaataat gagatatatt
     8941 acggtagagg atttgtcctt ctattatgat aaggagcctg ttcttgaaca tatcaattat
     9001 tgtgttgata gtggggaatt tgttaccttg actggggaaa atggagcggc taagacgacg
     9061 ctcatcaagg ctagtcttgg aattctgcaa ccacgcattg gaaaggtggc tatttcaaag
     9121 acaaatacgc aaggtaagaa attgagaata gcctatcttc ctcaacaaat tgccagtttt
     9181 aatgctggtt ttccaagtac ggtctatgaa tttgtcaagt cgggtcgcta tccgagaaaa
     9241 ggctggttcc gtcgtttgaa tgctcatgat gaggagcata tcaaggctag tctggactca
     9301 gttggcatgt gggaacatcg agacaaacgc ttggggtctc tatctggggg acaaaagcag
     9361 cgagcggtga ttgcgcgtat gtttgcttct gaccctgatg tgtttatcct agacgagccg
     9421 acaacgggga tggatgcagg aagtaaaaat gaattttacg aactcatgca ccacagcgcc
     9481 catcatcatg gcaaggctgt tttgatgatt acccatgacc ctgaagaagt taaggattat
     9541 gcggatcgca atattcatct agtccgtaac caagactcgc catggcgttg tttcaatgtt
     9601 catgagaatg gccaggaggt gggccatgct tagtttatta tcttacgact ttatacaacg
     9661 cgcctttttg gcggttattg ctatgagtct tttctcaccg gtattgggaa ccttcctcat
     9721 cttgcgtcgt cagagtttga tgagtgatac ccttagccac gtctcacttt caggtgtagc
     9781 ctttggtctg gttttgggga tttctccaac tgtttctact attgccattg tcttgattgc
     9841 ggcggtcttt ctggagtatc tccgtacggt ttacaagagc tttatggaaa tcgggacagc
     9901 tatcctcatg tcaacaggtt tggctgtttc tctgattgtc atgagcaagg gtagaagctc
     9961 gagttcaatg agtttggacc aatatctctt tggttcgatc gtgactatca gtgaagaaca
    10021 ggtcatttcc ctctttgtca ttgcggcggt tgttttgatt ttgacctttc tctttcttcg
    10081 tcctatgtat atcttaactt ttgatgaaga tacggccttt gtggatggct tgccagttcg
    10141 taccatgtcc attcttttta acatggtgac aggggtggct attgccctta tgattcctgc
    10201 agcaggagct cttctggtat cgaccattat ggtcttgcca gctagtattg ccctgcgtct
    10261 ggggaaaaac tttaaatcgg ttatgctgct tgccagtgcg attggctttt tgggaatggt
    10321 agcaggactt tacatttcct actatgcaga aacacctgca agtgcaagta ttaccattat
    10381 ttttgtaact gtctttatac taatcagttt agtaagacgt tttatcaaat aggagacgaa
    10441 cgtgaaaaaa attagcttat tactagccag tctatgtgcc ttgtttttag tggcttgttc
    10501 caatcaaaaa caggcagatg gtaaactcaa tatcgtgaca accttttacc ctgtctatga
    10561 atttaccaag caagtcgcag gagatacggc taatgtagaa ctcctaatcg gtgctgggac
    10621 agaacctcat gaatacgaac catctgccaa ggcagttgcc aaaatccaag atgcagatac
    10681 cttcgtttat gaaaatgaaa acatggaaac atgggtacct aaattgctag ataccttgga
    10741 taagaaaaaa gtgaaaacca tcaaggcgac aggcgatatg ttgctcttgc caggtggcga
    10801 ggaagaagag ggagaccatg accatggaga agaaggtcat caccatgagt ttgaccccca
    10861 tgtttggtta tcaccagttc gtgccattaa actagtagag cacatccgcg acagcttgtc
    10921 agcagattat cctgataaaa aagagacctt tgagaagaat gcagctgcct atatcgaaaa
    10981 attgcaagcc ttggataagg cttacgcaga aggcttgtct caagcaaaac aaaagagctt
    11041 tgtgactcaa cacgcagcct ttaactatct tgccttggac tatggactca aacaagtcgc
    11101 aatctcagga ctttctccag atgcagagcc atcagctgct cgcttggcag aattgacaga
    11161 gtatgtcaag aaaaataaaa tcgcctatat ctattttgaa gaaaatgcct cacaagccct
    11221 tgctaacaca ctttcaaaag aagcaggtgt caaaactgat gtcctcaatc ctttagaaag
    11281 tctgacagaa gaggacacca aggctggaga aaactacatt tccgtgatgg agaaaaacct
    11341 caaggctttg aaacaaacaa cagaccaaga aggcccagca atcgaacctg aaaaggcaga
    11401 ggataccaag acagtcccaa atggttactt cgaggatgca gctgtcaagg accgcacctt
    11461 gagtgactat gcaggtaact ggcaatcagt ttatcctttc cttgaagacg gcacgtttga
    11521 ccaagtcttt gactacaagg ctaagttgac tggtaagatg acccaggctg agtacaaggc
    11581 ttactataca aaaggctatc agacagatgt gactaagatt aacattactg ataatactat
    11641 ggaatttgtt caaggtggac aaagcaagaa atacacttac aagtatgtcg gtaagaaaat
    11701 tttgacttac aagaaaggca atcgtggcgt gcgtttcctc tttgaagcca cagatgctga
    11761 cgctggacaa ttcaagtatg ttcagtttag tgaccacaat gttgccccag ttaaggcaga
    11821 acatttccat atcttctttg gaggcacaag ccaagaagcc ctctttgaag aaatggacaa
    11881 ctggccaacc tactacccag ataacctatc tggccaagaa atcgcccaag aaatgttggc
    11941 gcattgatga gaaatcgact agttcataag agctagtcgg ttttttgata ctagatggct
    12001 aacttcatta tagacctttc agaactttaa acaataaata aaactcttga aatatctggg
    12061 cttatatgct agaatgtagt tagtgtataa ggttcattag aacaccaaag cccctaacta
    12121 gtgccatagt taagggcttt tttgacgtat cttagcgatt taacgggtgt ttataggcta
    12181 gtcagtcggt ctactcctta ctatctagcc atctgcagac gaaatacaag ataattccag
    12241 ctataacatc cgctataata gtaagagcga gctctatgat aaggctcatt agtttcacct
    12301 tctctcacga acccatagga acgtaatcgg taaccgatga caaaaatagt ataccacaat
    12361 aaatttagat catcaaggtc acttaattct tgaaatatag ggtctaggcg acaaagtctt
    12421 taaaatcaga aaaacgcata atatcaggtg tgcaaaaact tgatactatg cgttttattg
    12481 tgggaaggtt tactccattt tctcctgaaa ttgagttttt gtccagcctc tgtttttggg
    12541 gttgctaaga aaataatgtc atgtggtgaa tatttgtaaa tcagtcagca gacagaacga
    12601 atactcttcg aaaatctctt cacatcatgt cagcttcgtc ttgccgtgtc catgggggac
    12661 ttgacttcat cagttctatc tacaacctca aaacagtgtt tcgagctgac ttgatcaatt
    12721 ttcaaatctg tactttgagc aagctgagac tagcttccta tttgattttc attgaatatc
    12781 agaaacccat tctccatcaa ataattcgac tgcgtctaat aatttttgat ctggcacggt
    12841 gtctgaaata aaggttgtgt atttggagag gggattaatt ttaaaaaatc cagtcttgta
    12901 aaatttagaa ctatcaatca gtaagatggt ttcatgggct ttgtcaataa tattcttttt
    12961 tgaaatagct tggctgagag aagcttcata aacatattgg tcatcaatac ctcttgctga
    13021 acaaaatgct aaatcgatat taaaatgatc taataaagaa ttttccttat catagttgac
    13081 cacggaacag gattgatgtt tgacctcgcc agatgtgata aagattttgg agctatcttt
    13141 aacagtttca gatagggttt gtgcagtatg taaaccattt gtaaaaataa tcaaattatc
    13201 aagttcagaa agatagggac aaagttcgta gacagtcgta ctggaatcca gatagataca
    13261 cataccagac cgaataaagt ctttagcgag actagcgatt agtctttttt gcctagtact
    13321 ttctccttca cgtatttgat gagaaagttc aattgtgttc atagaggaca gggtcacgta
    13381 tccgtgcttt cttttgataa gaccttgatt ttctaagaaa attaaatcac gacgtaaggt
    13441 acttgtgctg gagaaagtga tttctgccag ctcttttacg gcaattcttt ttttcttttt
    13501 aataatttca atcaattcaa gtacacgttc atcttttatc ataagctcct cctaatttat
    13561 catttcaact atattatagc acaaattgga ggaatttgaa ttatttttat gaatattggg
    13621 ttaatatttg aacattattc aagtaagcgt taatatattg aaaaaataaa acgtggggat
    13681 tataataaag ttaatcaagg acgaagagag aagaaaaatg gaagcggttt tagcaataga
    13741 tttaggtgcg acttctggaa gagcaatcgt tggttacctt tctgaaaata aactagtaat
    13801 ggaagaaata aatcgctttt ctaatctacc tattagagta aaagggcatt tatcttggga
    13861 tattgacttt ctactagcta aaattcttga aagtatccgc ttggctaata ctagttacaa
    13921 gattttatct atcggtattg acacatgggg agttgatttt ggactgattg ataatgaagg
    13981 taagctgtta tcacaacctg ttcattatcg tgatgaaaga acaaagggag tgttaaagga
    14041 aatatctgaa atgactgaat tagaaaaact gtattcagag acaggaaatc agattatgga
    14101 gataaatacc ttgtttcaac tctttaaggc acgtcaagaa tctcctgact ctttctataa
    14161 gaccaataag attcttttaa tgccagattt gtttaattat ctcttgacag gtaagtttgc
    14221 tacagaaaaa agcattgctt caacaactca attatttgat cctaggagtc aaaattggaa
    14281 tcagaatatc ttaaaactat ttgaattgga ttcatcttta cttcctgaaa ttgtttcaga
    14341 gggaaatgtt cttggaagga taaaaaagga gtatggttta ggcgatattc ctgttgtgaa
    14401 tgtttgtagt catgatacag caagtgcgat tgtctcagta cctaagacag aaggtagttt
    14461 atttatttca tcaggtactt ggtctttggt tggagtggaa cttacttcac cgattcttac
    14521 taccgaatcc ttcagttatg gatttacaaa tgaagtcggt aaagatggag tgattacatt
    14581 tctgaagaat tgtacagggt tgtggatcat agaggaacta agacgttcat ttgaacgaag
    14641 agggaaagcc tattcttttg atgatattag gacaatggtg gagaaagaaa aagaaaatct
    14701 tcctctgatt gatactgaat caactgaatt tgcaacagaa tctgatatgc acaagacttt
    14761 gacagaatat ctagcttatc atcatgaaac tagagagtgg acagatggac aactatttaa
    14821 gattgtttat gaaagcctag ctgaaacgta taggaaagcg atagagttac tagaagaact
    14881 aactcataag gtttataaga ggatatatgt gattggagga ggtgctagag ccagttactt
    14941 taaccaaatg attgctgata gaactggtaa agaggttctt acaggtttga ctgaggctac
    15001 agctgtgggg aatattgttg tgcagctcat agctatggga caattaaaag ggatggaaga
    15061 ggctcaccat gttattgagg agtttctaca attagagagt tattactccc aaaagaatta
    15121 aaaagattga gagtttgtaa atttgcctcc ctcccccttc ttagcttttg tgcaggaagg
    15181 ggggataatt ggtgaattga aaaatattta gtgttttgat atgaggagga caaggatgtc
    15241 agatgtaaaa caagaattaa ttaaatatgg taagaagcta gtagaaacag atttgacgaa
    15301 aggaacaggt gggaatctca gcgttttcga tcgtgaaaaa caattgatgg caattacccc
    15361 gtcgggtatt gatttctttg aaatcaaaga atccgatatt gtagtgatgg atattaatgg
    15421 aaatgttgta gagggagaac gcttgccatc tagcgaatgg tatatgcatt tgattcaata
    15481 tcaaactcgt gatgatatcg atgcaattat ccatgctcat acaacttatg caacagtatt
    15541 agcttgtctc agagaaccac ttccagcgag tcattatatg attgcagtgg cagggaaaga
    15601 tgttcgggta gctgagtatg caacatatgg cacgaaagaa ttggctgtga atgcagctaa
    15661 agcaatggaa ggtcgtagag cagttttact agcgaatcat ggaattttag caggtgcaca
    15721 aaatttattg aatgcattta atattgttga agaagttgaa tattgtgcaa aaatttattg
    15781 tttagctaag aattttggag agccagtagt tcttcctgat gaggagatgg aattgatggc
    15841 agaaaaattt aaaacatacg gtcagagaaa atagggagga tattaatgtt aaaacatata
    15901 ccgaaaaata tttctccaga tttattgaag actttaatgg aaatgggaca tggagatgaa
    15961 atagtattag ctgacgcgaa ttatccttct gcctcatgtg caaataagct aattcgttgt
    16021 gatggcgtaa atattccaga attattagat tccattctgt atttaatgcc attagatagt
    16081 tacgtcgata gttcaattca gtttatgaac gttgtttcgg gtgatgatat tcctaagata
    16141 tggggtacct atagacagat gattgaaggt catggtacag atcttaaaac gattacttat
    16201 cttagaagag aagattttta tgaacgtagt aagaaagctt atgctattgt tgctacagga
    16261 gaaacttcac tttatgctaa tattatcctt aagaaaggag tagttgttga aagagaaaat
    16321 gtttaataga ggaattttag ttgccagtca tggtgatttt gctagcggag ctctcatgac
    16381 cgcagaaatg tttgttggtg agacaacaaa tgatagagtt aggacattag gtttgatgcc
    16441 tggagagaat attgtagagt ttgagcatta ttttaaaaat caagtggatg aactgttaga
    16501 ctcaaatcaa gaggttatcg ttttgactga cttgattgga ggaagtccta ataatgtggc
    16561 tttgtcacgg tttttaaatt tggattcagt tgatattgta acagggttta atattcctct
    16621 cctagtggaa ttaatatcaa attatgattc aaaaatcaat ttagaagaaa ttgttcacaa
    16681 tgctcaaaat agtttgttta atgttaaaca acaacttaac gtagaggagg aagaagattt
    16741 atgtctatag agtttgttcg tattgatgac cgtctggtac atggtcaagt tgtcactacg
    16801 tggctaaaaa agtatgatat tgagcaagtt atcattgtta atgatcgcat ctcagaagat
    16861 aaaacacgac aatctatttt aaagatttct gcaccagtag gtttaaaaat tgttttcttt
    16921 agtgtaaaac ggtttgtgga agttttaaac tctgtgccaa taaaaaagag aacaatgctg
    16981 atatatacaa atccaaaaga tgtgtatgat tctattgaag gaaatttaaa attggagtac
    17041 ctcaatgtag gacagatgag taaaacggag gaaaatgaaa aggtaacggg aggtgtagct
    17101 ctaggtgaag aagacaaata ttattttaag aaaatagttg ataagggaac gagagttgaa
    17161 attcaaatgg ttcctaatga taaagttaca atgttggaaa aatttttata aaaataattt
    17221 aaggaggtac agtatatgct attcacacaa gcattactgg tgacattagt tgggattatt
    17281 gccactattg actataatgg accgttattt atgattcacc gtccgttagt tacaagtgca
    17341 atggttggct tagtcttagg agatttcacc caaggtgttc ttattggttc agctcttgaa
    17401 ttaacttggc tcggtgtaac aggtattgga ggttacactc caccagatac tatttcaggt
    17461 gcgattattg gtactgcatt tggtatttta tctggtcaag gagaaactgc tggtatcgct
    17521 atagcagttc caattgcagt tgctacccaa cagttggatg ttcttgcaaa aactttagat
    17581 gtttattttg tgaaaaaagc tgataatgat gctaaaaacg gagattattc aaagatcggt
    17641 ttttatcatt attcaagttt ggttttaatc acgttattta aaattgtacc aattttccta
    17701 gctattatgc ttggagggga atatgtggca gacttgtttg ctaaggttcc accaatcgtt
    17761 atgcagggac ttaactctgc aggtgcttta ctaccttcaa ttggttttgg tatgctttta
    17821 aatatgatgc tcaagaaaaa tatgtgggta ttcttgttga ttggattcat ttgttctgtg
    17881 tatggaggaa tgtcaaccat tgggatctca ctagttggta ttgcggtagc atacttctac
    17941 gatatgattg gaagtaaacc acaagaaaca acttcaagta gtgatgttga ggaggatctt
    18001 gatctatgat gaataataaa gtaactaaag ttgaacttaa aaaagttttc aaacgaagtt
    18061 ttatgtatgg ttcttcatgg aactatgaga gaatgcagaa cctaggtttt ctatatacaa
    18121 ttcttccagt attgaaaaaa ctatacccag acaaagattc agcttctcct gcaatgaaac
    18181 gtcaccttga gtttttcaat actcatcaaa cagcggcacc atttattctt ggagttactt
    18241 ccgctatgga agaacaagaa ggaaatgaag gtgcagcttc aattactggt attaaagttg
    18301 gcttgatggg gccactggct ggtctaggag atagtttgtt ctggctgaca ctagttccta
    18361 tctgttttag tattggtgcg tcttattcta aagacggcgg tgctttaggt atctttatcg
    18421 ccttaatatt gtttaatatt attaatattc ctgttaaata tttcggtttg aaatatgggt
    18481 atactaaggg ttctagtctt atccaagaaa ataatacaaa aggaacattg aatcgcgtta
    18541 cgagtatggc gacagcatta gggctagtac tagtgggtgg tttgattcca tcaatggttg
    18601 gtattaattt tggattagaa tttaagcagg gggaacttgt tatttctgtt caagaaatga
    18661 ttacaaaatt aattccagga tttatcccta tggctttgac tttattaatg tgtaaattaa
    18721 ttagaaaagg aaagaatccg gttgtactaa tctttagtgt tatggctatt ggagttatcc
    18781 tagttgtttt aggaattttg aagtagtaga aagtgtggag gtggtatttg ggatatcacc
    18841 tccattttgg aagagaggta aagagtgaaa ttatggtata agaaagctgc ctcaaattgg
    18901 aatgaagcct tgccgattgg gaacggtcat ttaggtggta tgatttatgg ttcagctaca
    18961 aaagaatgta ttcaactaaa cgatgagact atttggtata gaggaaagtc agatagaaat
    19021 aatccagact cactattgca tcttaaaaaa attcgggaat atcttttaga tggagaaatt
    19081 cagaaagccg aagaattgat aaagttaaca atgtttgcta ccccaagaga tcaaagccac
    19141 tatgaattac ttggggaact ttacattgag catatagata ttcagtcttg tgctctttca
    19201 ttgtatgaaa gagagctaga tttagataca gctatttcta atgttgtgtt tgagcctaat
    19261 agttgtaatt tacaaataaa aagagaatat tttacgagtt ttaataagaa tattttatgt
    19321 tgccgtatag tgtcatcagt tcaaaacaca ttaaatttaa acattaattt gggtagaaat
    19381 aaacggttta atgacgaagt atctaaactg gattcaagta caa
//
LOCUS       DAVLYV010000033        17957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLYV010000033 DAVLYV010000000
VERSION     DAVLYV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.033"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            331..1377
                     /gene="pheS"
                     /locus_tag="VYO70_001149"
     CDS             331..1377
                     /gene="pheS"
                     /locus_tag="VYO70_001149"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1713388.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHHHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1377..1886
                     /locus_tag="VYO70_001150"
     CDS             1377..1886
                     /locus_tag="VYO70_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713389.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1963..4368
                     /locus_tag="VYO70_001151"
     CDS             1963..4368
                     /locus_tag="VYO70_001151"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1713390.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIEFSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVSLLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(4541..5542)
                     /locus_tag="VYO70_001152"
     CDS             complement(4541..5542)
                     /locus_tag="VYO70_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1713391.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            5698..6101
                     /locus_tag="VYO70_001153"
                     /pseudo
     CDS             5698..6101
                     /locus_tag="VYO70_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6227..6493
                     /locus_tag="VYO70_001154"
     CDS             6227..6493
                     /locus_tag="VYO70_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713392.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGISQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            6754..9003
                     /gene="metE"
                     /locus_tag="VYO70_001155"
     CDS             6754..9003
                     /gene="metE"
                     /locus_tag="VYO70_001155"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1713393.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVSAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEVILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            9067..9933
                     /gene="metF"
                     /locus_tag="VYO70_001156"
     CDS             9067..9933
                     /gene="metF"
                     /locus_tag="VYO70_001156"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1713394.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQTPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            10620..12833
                     /gene="pnp"
                     /locus_tag="VYO70_001157"
     CDS             10620..12833
                     /gene="pnp"
                     /locus_tag="VYO70_001157"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1713395.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYANHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMTGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            12849..13466
                     /gene="cysE"
                     /locus_tag="VYO70_001158"
     CDS             12849..13466
                     /gene="cysE"
                     /locus_tag="VYO70_001158"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1713396.1"
                     /translation="MGWWHETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KYDFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            13478..14362
                     /locus_tag="VYO70_001159"
     CDS             13478..14362
                     /locus_tag="VYO70_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713397.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGKLVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYQLAKLSNLEVLLADSSY
                     QEQISQLKFQAFSGEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSSDT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            14449..15792
                     /locus_tag="VYO70_001160"
     CDS             14449..15792
                     /locus_tag="VYO70_001160"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1713398.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFEDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFETADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            15785..16171
                     /locus_tag="VYO70_001161"
     CDS             15785..16171
                     /locus_tag="VYO70_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1713399.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            16175..17059
                     /locus_tag="VYO70_001162"
     CDS             16175..17059
                     /locus_tag="VYO70_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1713400.1"
                     /translation="MREKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGSQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            17196..17795
                     /locus_tag="VYO70_001163"
     CDS             17196..17795
                     /locus_tag="VYO70_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713401.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNLSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNTK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
BASE COUNT         5417 a         3459 c         4055 g         5026 t
ORIGIN      
        1 tcatcttatt tcaatccgct ataaagttag ataagaataa gctaacaagc agaagtaaga
       61 taggtcttgc aagaatggta aaaatctgat aaactaagta gtaattgaat aggaatctga
      121 aagactagta gcccaaggga agtatatcag ggaggagagc cgtgactgca agctctctat
      181 atgaaagctg ggtgaattca cttgcgcatg gatttagaaa tgaaggtctg acttgtcaga
      241 tgaaaacgga tggtaccgcg tgtcaacgct ccgagtggag tttttggcat gtggttttct
      301 ttttatctac gagagactga tggaggaaat atgtcaacta ttgaagaaca attaaaagcg
      361 cttcgcgaag aaacgctggc tagcttgaag cagattactg ctggaaatga aaaagagatg
      421 caagatttgc gtgtctctgt ccttggtaaa aagggttcgc tcactgaaat cctcaaaggg
      481 atgaaagatg tttctgctga gatgcgtcca atcatcggga aacacgtcaa tgaagctcgt
      541 gatgtcttga ctgctgcttt tgaagaaaca gctaagctct tggaagaaaa gaaagtcgcg
      601 gctcaactgg ctagcgagag tatcgatgtg acgcttccag gtcgtccagt tgcgactggt
      661 caccatcacg ttttgacaca aaccagtgaa gaaatcgaag atatcttcat cggtatgggt
      721 tatcaagtcg tggatggttt tgaagtggag caagactact ataactttga acgtatgaac
      781 cttccaaaag accacccagc tcgtgatatg caggatactt tctatatcac tgaagaaatc
      841 ttgctccgta cccacacgtc tccagttcag gcgcgtgcta tggatgccca tgatttttct
      901 aaaggtcctt tgaagatgat ctcgccaggg cgtgtcttcc gtcgcgatac ggacgatgcg
      961 acccacagtc accaattcca ccaaatcgaa ggcttggtag ttgggaaaaa tatctctatg
     1021 gctgatcttc aaggaacgct tcagttgatt gtccaaaaaa tgtttggtga agagcgtcaa
     1081 attcgtttgc gtccatctta ctttccattc acagagccat ctgttgaggt ggatgtttct
     1141 tgcttcaaat gtggtggaga aggctgtaac gtatgtaaga aaacaggttg gatcgaaatt
     1201 atgggggccg gtatggttca cccacgtgtc cttgaaatga gtggtatcga tgcgactgta
     1261 tactctggct ttgcctttgg tcttggacaa gagcgtgtag ctatgctccg ttatggaatc
     1321 aacgatatcc gtggattcta ccaaggagat gtccgcttct cagaacagtt taaataatga
     1381 ttagaaaagt agaaatggca gatgttgagg tgttggctaa aattgccaaa caagcctttc
     1441 gtgaaacctt tgcgtatgat aatacggaag agcagttaca ggaatacttt gaagaggctt
     1501 atagtctgaa aactttgtca actgagttgg gaaatcctga ctctgaaacc tatttcatta
     1561 tgcatgagga ggagatagct ggttttctca aagtcaactg gggaagtgct caaactgaga
     1621 gagaattaga ggacgctttt gaaattcaac gcctctatgt gctacaaaaa ttccaaggat
     1681 ttggactagg taagcaactg tttgaattcg cacttgaact tgctacaaaa aatagttttt
     1741 cttgggcttg gctaggtgtt tgggagcata atacaaaagc tcaagcgttt tataaccgat
     1801 atggttttga aaaatttagc caacatcatt ttatggttgg tcaaaaagta gatacggatt
     1861 ggttactgag aaagaaatta aggtaagaag agaattcttc tattgaaatg ataggaaagg
     1921 aaaagaacca gtgtggcaat ggtcattctg gagaactaaa ttatgcttgt atcttataaa
     1981 tggttaaaag aattggtggg cattgatgtg ccatcacagg agttggctga aaaaatgtca
     2041 accacaggga tcgaggtcga aggtgtcgaa tcaccagctg ccggtctttc aaaaattgtc
     2101 gtcggtgaga tcttgtcctg cgaagatgtg ccagaaactc accttcatgt ttgtcaggtt
     2161 aacgttggcg aagaagaaga gcgtcagatc gtttgtggtg ccccaaatgt gcgtgcaggc
     2221 atcaaggtta tggtggctct tccgggagct cgtatcgctg acaattacaa aatcaaaaaa
     2281 ggaaaaatcc gtggcttaga gtcacttgga atgatctgtt cacttggtga attgggtatt
     2341 tctgactcag ttgtacctaa gaaattcgca gatggtattc aaattttgcc agaaaatgcc
     2401 gttccaggtg aggaagtctt ttcatatcta gacttggatg atgaaatcat cgaattttct
     2461 atcacaccaa accgtgcaga tgccctttct atgcgtggag tggctcacga agtggcagcc
     2521 atatatgaca aggcagtcaa ctttaaagaa tttactttga cagaaacaaa tgaagctgcg
     2581 acagatgccc tttctgtggg cattgagaca gacaaggcgc cttactatgc agctcgtatc
     2641 ttggacaatg tgactatcgc accaagtcca caatggttgc aaaaccttct catgaacgaa
     2701 ggcatccgtc ccatcaataa cgttgtagac gtgacaaact acatcctgct ctactttggt
     2761 caacctatgc atgcttttga cttggacaca tttgaaggga ctgacatccg tgtgcgtgaa
     2821 gcgcgtgctg gtgaaaaatt ggtgaccttg gacggtgaag aacgtgactt ggacgtgaat
     2881 gacctagtca tcactgttgc agacaagcca gtagcccttg caggtgtcat gggtggtcaa
     2941 gcaacagaaa tctctgaaaa atctagtcat gttgtccttg aagctgctgt tttcaatggc
     3001 aaatctatcc gtaagacaag tggtcgcctg aaccttcgtt ctgagtcatc ttctcgcttt
     3061 gaaaaaggaa ttaatgtggc aacagttaat gaagcccttg atgcggcagc tagcctgatt
     3121 gcggaacttg caggtgcgac ggtgcgtaag ggtatcgttt cagcgggtga gcttgatact
     3181 tcagatgtag aagtttcttc aactcttgct gatgttaacc gtgtcctcgg aactgagctg
     3241 tcttatgctg atgtcgaaga cgtcttccgt cgtcttggct ttggtctttc tggaaatgca
     3301 gacagcttta cagtcagcgt accacgtcgt cgttgggata tcacaatcga agctgatctc
     3361 tttgaagaaa tcgctcgtat ctatggatat gaccgcttgc caaccagcct tccaaaagac
     3421 gatggtacag ctggtgaatt gactgtgaca caaaaactcc gccgtcaagt tcgtaccatt
     3481 gctgaaggag caggtttgac agaaatcatc acctatgctc taacaactcc tgaaaaagca
     3541 gttgagttta cggctcaacc aagtaacctt acggaactca tgtggccaat gactgtggat
     3601 cgttcagtcc tccgtcaaaa tatgatttca ggtatccttg ataccgttgc ctacaacgtg
     3661 gctcgtaaga ataaaaactt ggccctttac gagattggaa aagtctttga acaaacaggt
     3721 aatccaaaag aagaacttcc aaatgaaatc aacagttttg cctttgcctt gacaggcttg
     3781 gttgctgaaa aagatttcca aacagcagca gttccagttg atttcttcta tgctaaagga
     3841 atcctagaag cgctctttac tcgcttggga ctagaagtga cctatacagc aacagctgag
     3901 attgccagcc ttcacccagg acgtacagca gtcatttcac tcggtgacca agttcttggt
     3961 tttcttggcc aagtgcatcc agtcactgct aaggcttacg atattccaga aacgtatgtg
     4021 gctgagctca acctttcagt catcgaagct gcccttcaac ctgctgctcc atttgtggaa
     4081 atcacgaaat tcccagcagt cagccgtgac gtttcccttc tcctcaaggc agcagtaact
     4141 caccaagaag ttatagatgc tatccaagct gccggcgtga aacgtttgac agatatcaaa
     4201 ctctttgacg ttttctcagg tgagaaattg ggacttggta tgaagtcaat ggcttatagc
     4261 ttgaccttcc aaaatccaga agacagctta acggacgaag aagtcgcacg ctacatggaa
     4321 aaaatccaag catcgcttga aaaaaaagtg aacgcagaag tgcgttaaac gatagtagga
     4381 tacaaattcc ccctctagtt tatgaacact agaggggtga ttttatataa tactcaatga
     4441 aaatcaaaaa gcaaactagg aagctagccg taagctgtac ttgagtactg taaggcgagg
     4501 ctgacgtggt ttgaagagat tgtcgaagag tataaaataa ttaaaatgtg atttctgtaa
     4561 aaatacatct atgatcagag ttttgaaaac ttacaatgtc taaatcttta acatagtagt
     4621 ggtttttagg caataaaata tgatctattg ttgcattaaa aagttttgga ctttggctat
     4681 tccaagttcc tctttcaaaa ggtggtagta catttaatgc gtccctatga gagcttattt
     4741 ttgcaagtgc tccatgacgc atagttgcat taaaatcacc tgcaataata gcctttggat
     4801 attttgaagc caattgatta tgaatgatgt ttaagtcttg cttccagatt tccattaaac
     4861 ctggcagagg aggagcagta tgcaaggcaa tgatatctgg tatattttgt tttctcgaat
     4921 gtaatacaat tgtcccgaac cgtgttgtat gaaaagtttt agcttctgta tagaaaccat
     4981 aacttttctt gacaatcaca gtcacaggag ctattccact attggtaggt ggagaagtga
     5041 aaatatcata gttggccata gaaagtccaa cttgatgaaa caatagtttg attctctggt
     5101 tttcttgctc acctctgata ttggtagcta gttcagggaa tatagccata tcggcgtcaa
     5161 aatggctaaa aattcgctca atatgttgtg cttctatttg attagcgacg ttccaagtta
     5221 ctaattttaa ggttttagtc ttatttgatg cagaagaaag ggttttatct gttccgaata
     5281 atagtaatat gagcgatagt aagagcaaaa tgccagagta aaaccgtatt tgtttatttt
     5341 tcttgtaaaa agatagggaa aaaaatacga aacttaaaat agcagccata gttgcagtag
     5401 ctaaggggaa ggcataaatc tgtgccaaga taggtctggt agatagccaa atattaagat
     5461 tagaagatgg aaaaaaagaa taagttatga tagggataac aaagagaatg accgaaaggg
     5521 cagagagaaa tgttttaatc attgtgtgag tacctcaatt tgcaatggtt gctcggagct
     5581 aatgtgactt tgtgacttta cttgattgat attctttttc ttgtcttcct catagattaa
     5641 gtacaaccca agtaaaaaaa gtccaattta agattgaaat gattaaaagg cacagaaatg
     5701 caagacaatt atactacaaa aggcaaacat ttgacaatcg atagccgtcg cttaatcgaa
     5761 agatggaaaa aagaagggaa atcaaataga gaaattgcct ctctgcttgg aaaagttcct
     5821 caaactatcc acactgaaat caagcgtggg acagtccgac aatgtcttgg aaaagggcgc
     5881 ttcaaagagg tttattctgc cgactacgct caacagtctt aagaaactaa aggaaaagat
     5941 tctccactat acataaccaa aaattttcgc ctgaaatgat ggttatggtt aaaggggtta
     6001 acgtgggaat ttcaaccatt tactattgga ttcatcgtgg aaaattgggg ttaagcaagc
     6061 aggatctgct ttatcctaga aaaggaaaat cgcttaagaa ataggctagc accaacttta
     6121 aacccactgg tcaatctatc gaatcataat tcgctcattt ttttaaaaat acatagacgt
     6181 aaaaaaaaga gggttaagtg atgaagaata gtgctattgg gagtaattgg aaggatgtcc
     6241 atctgagctc tttagcaagg aggaaatcct tgaaagtgat atgcgagtgg ctatcatgag
     6301 cgagttgatt gaggctaggt atgaacaagg aatcagtcag aaaaagctgg aagaagtcag
     6361 tggaataagc cagcctgtca tagctaggat ggagacagga aagactagtc ctcagttgga
     6421 tacagtctta aaagtcctag ccagtttagg aaagacacta gcagtcgtcc cacttgaaca
     6481 gggaaaaagt tgactagaat cagttatagt cttttctaat aacaggtcat agtcacttat
     6541 aagaattact aataacatgt tttagtattc taattggaat atagctgtaa agtaagaatt
     6601 acaaaggatt tgagacgttt gcttcaagtc tttttctttt gtccgaaaca gatatatagt
     6661 ttcaaactat atcaaaccct aattttttac aattatacag acgctttctt ttctgttaag
     6721 atagtttcaa caacaaattt tggaggacac atcatgtcaa ctacaatcat cggtttccct
     6781 cgtttgggcg aattccgtga attaaaattt acaactgaaa aatactttag aaaagaaatc
     6841 tcagaagaag aacttttagc agcagcaaaa gacttgcgtg ctaaacactg gaacattgtc
     6901 aaagaaaaag gcatcactga aattccatca aatgactttt ctcactatga taacttccta
     6961 gatgcggctt tccttttcaa cgtggtgcct gcatccgttc aaaacttgga cttgtctgac
     7021 cttgagcgct acttcgcttt gggacgtggt taccagggag aaaaagggga cgttcgcgcc
     7081 cttccaatga agaaatggtt caacactaac taccactaca tcgttcctaa atttgaaaaa
     7141 gacactcaag taaaattggc tggtcacaag attttcgatg agttccaaga agccaaagaa
     7201 cttggattga acactcgtcc tgttcttgta ggtcccttca ctttccttca attgtcagac
     7261 tttgaagaag gcgtgaaagc agacgacttc gtagatagct tagtgtctgc ttaccaagaa
     7321 gtttttgcta aattggctga acttggtgcg actcgcatcc aattggatga agcggctctt
     7381 gtaaaagact tgacagctga agaaaaagct cttttcttga acctttacaa caaactcttg
     7441 gctgacaaaa aaggtcttga agtcttgctt caaacttatt tcggtgatgt tcgtgacgta
     7501 tacgctgacc ttgtaaactt accagtagat gctatcggtc ttgactttgt tgaaggtaag
     7561 aaaactcttg aactcgttaa aggtggcttc ccagctgaca agactctcta tgtaggtatt
     7621 gtcaatggta aaaacatctg gcgcaacaac tacgaaaaga gcttgactgt tcttgagcaa
     7681 atcccagctg aaaacatcgt tttgacaagc tcttgctcac ttcttcatgt gccatttaca
     7741 actgctaatg aagaatttga accagcactc ttgaaccact ttgcctttgc agttgaaaaa
     7801 ttggatgaaa tccgtgactt ggatgctatc cgaaatggtc aaggttcaga agctcttgca
     7861 gccaacaaag aactctttgc gactgagcgt gttggtgaaa atgctgaact tcgtgcgcgt
     7921 atcgctggct tgacagacgc agactacact cgtttgccag cctttgcaga acgtgaagct
     7981 atccaagaag aagctttcaa acttccagct cttccaacaa caacgattgg ttcattccct
     8041 caaacaaaag aagttcgtgc caaacgtttg gcttaccgta aaggtgaatt gtctcaaaaa
     8101 gagtacgacg ctttccttgc tgaaacgatt gacgaatgga tcaagtggca agaagatatt
     8161 gactttgatg tccttgttca cggtgaattt gagcgtaatg acatggttga gtacttcggt
     8221 caaaacttgt caggatacct cttctctaaa aatggatggg tacaatcata cggtatgcgt
     8281 ggggtaaaac caccaatcat ctggggtgat gtcactcgtc ttaaccctat cactgttaaa
     8341 tggtctagct atgcacaaag tcgtacaaac aaacctgtta aaggtatgtt gactggacct
     8401 gttaccatcc ttaactggtc attcccacgt gaagacatct ctatcaagga ttcaactctc
     8461 caaatcgccc ttgctatcaa ggatgaagta cttgaccttg aagctgctgg tgtgaaaatc
     8521 atccaaatcg acgaggctgc tcttcgtgaa aaattgccac tccgccgtag cgactggtac
     8581 gaagactacc ttgactgggc tattcctgcc ttccgcttgg tacactcaac agtagcgcca
     8641 gacacacaaa tccacactca catgtgttac tcagaattta cagatatcat cccagctatc
     8701 gataacatgg atgcagacgt tatttccttt gaagctagcc gttcaaacct tgaaatcttg
     8761 gacgaactca aagcgaaaaa cttccaaaca gaagtgggac ctggggttta cgatatccac
     8821 tcacctcgtg taccaaatga aggcgaaatc gacaacacaa tcgaagtcat ccttgctaaa
     8881 gtgccaagca agaaagtttg gatcaaccct gactgtggtt tgaaaacacg tggtatccca
     8941 gaaacaaaag aaagcttgat ccgccttgtt gaagcagcta aagctgcgcg tgagaaattg
     9001 taagattgga tttctctcca tacagtggtt gttcaaaaag aaatcaacta gaaaaggtta
     9061 ttacatatgt cacgccaaac accgtcactc tcatttgaag tgttccctcc aaacccagcc
     9121 gtgggtaatg ataacattat ttcagccttg caggatatgc aggagttggc tccccatttt
     9181 atcagtgtaa ctgccagcaa taataaattt aatatcaagg aaacgacggt tcgtttggct
     9241 gactttatcc aaaatgattt ggcgattccg accattgccc acttgccagc tatttatcta
     9301 actaaggaca aggttgctga aactattgct gacttggaca aagttggggt gcagaaaatc
     9361 ttggctcttc gtggggatat tattccagac gtggaaccac aaaaggattt ccgctatgca
     9421 acagacttga ttgagtttat caaggaacaa acccctcact ttgatattat tggagcttgc
     9481 tatccagaag ggcatccaga ttcgccaaat cagatttcag atattcaaaa tcttaagaag
     9541 aaagtggatg caggctgttc gagcctcgta actcagctct tctttgacaa tgagcgcttc
     9601 tatgattttc aggacaagtg catcttggct gggattgatg ttcccattca tgcaggaatt
     9661 atgccaattc tgaatcgaaa tcaggctctc cgactcttga agacttgtga gaatatccat
     9721 cttccacgca aatttaaagc catcttagac aagtatgagc atgaccctga gtcgctcaga
     9781 gcagcaggac ttgcctatgc agtggaccaa atcgtggact tggtaactca ggatgttgcc
     9841 ggtgtgcatc tctatacgat gaataatgct gatacagcaa aatacatcca tcaagcaacc
     9901 catgccttgt ttaatcacca gtctctagga taataaaagc aaaccattct tctcaggtga
     9961 ggggaatggt tcctttttaa tggtaaagac ctcacttttt aagaaaaata tgataaaata
    10021 aattctgtac gtacttgata caaagatgaa ggtattaaat aatacaatgc atttaaactg
    10081 taagatttaa cgacacgagt gagtcaaatc gatgttattt gacgagagag ttagtaaagc
    10141 atttaactaa tcgcccgata gcgattagcg tgaaagcttt agatacttta gtattaaacc
    10201 tttctaatga tttatatctt gaaaactgaa ctcgcctaca actctgtgga aaaagataaa
    10261 tctccctagg agcagtcgct ccgtcgttcg atttcctatt ttcctttgag ttgcttgacg
    10321 gcttagtatc ttgagtaatt gaattttggt ctcatttctt aggaaaaaag ataagcttcc
    10381 tagcactcgt cgagtgcgac gtcaccttcc tatttttcta cagaaatgtt cggcaagccg
    10441 aaccgtccaa aatatcttgt gtaattgaac acggccgaaa agctgtgtaa aaaagataaa
    10501 ctgtcttgtc ttcatcgaag acttcgtcag tttcctattt ttactttgct tttgacgtcc
    10561 ttggtatctt gatctttgta ggcaaggcgt ataatttcat caatccaaag gggattaaaa
    10621 tggcaaaaca agtgtttcaa acgacttttg cgggtcgtga gttaattgta gaaactggtc
    10681 aggttgctaa gcaagcaaat ggctctgttg tcgtacgtta cggtgagtca actgtcttga
    10741 ctgctgccgt tatgtctaag aaaatggcaa ctggggattt cttcccactc caagtcaact
    10801 acgaagaaaa aatgtatgcg gctgggaagt ttcctggtgg ctttatgaaa cgtgaaggac
    10861 gtccttcaac agatgcgacc ttgacagcgc gtttgattga ccgtccgatt cgtcctatgt
    10921 ttgcggaagg tttccgtaat gaagtccaag tcatcaatac agtgctttct tatgatgaaa
    10981 atgcatctgc accaatggct gctatgtttg gttcatcttt ggcactgtct atttcagata
    11041 ttccatttga cggaccaatt gctggggtac aagtgggata tgtagatggc caaatcatca
    11101 tcaacccaag tcaagaacaa gcagagcaat ctcttcttga attgacagta gctggaacca
    11161 agcacgctat caacatggta gagtctggtg ccaaagaatt gtcagaagaa atcatgttgg
    11221 aagcgcttct taaagggcac gaagctgtca aagaattgat tgccttccaa gaagaaatcg
    11281 ttgctgctgt cggtaaagaa aaagcagaag tggaattgct tcacgtggat gctgaattgc
    11341 aagctgaaat cattgcagcc tacaacagtg acctccaaaa ggcagttcaa gtagaagaga
    11401 aattggcccg tgaagctgca actcaagcag tgaaagacca agtgactgcc gtttacgaag
    11461 aaaaatatgc gaaccacgaa gaatttgacc gtattatgcg tgatgtggct gaaatcttgg
    11521 aacaaatgga acacgcagaa gtgcgacgtt taattacaga agacaaggtg cgtcctgatg
    11581 gtcgtaaggt cgatgaaatc cgtcctttgg atgcggttgt tgacttcctt cctcgtgtac
    11641 atggttcagg tctctttact cgtgggcaaa ctcaagctct ttcagtcttg accttggctc
    11701 cgatgggaga aactcaaatc attgatggtt tggatccaga gtacaagaaa cgctttatgc
    11761 accactataa cttccctcaa tattctgtag gggaaacagg tcgttacggt gcgccaggtc
    11821 gtcgtgaaat cggtcacggt gcccttggtg agcgtgctct tgctcaagtc ttgccaagct
    11881 tggaagaatt cccatacgct atccgtctag tagcagaagt tttggaatca aacggttctt
    11941 catctcaagc ttctatctgt gcgggaactc ttgcccttat gactggtggt gtgccaatca
    12001 aggcgccagt agctggtatt gctatgggac ttatctcaga tggaaataac tacacagtat
    12061 tgacagatat ccaaggtttg gaagatcact ttggagatat ggacttcaag gttgcaggta
    12121 ctcgtgatgg gattacagcc cttcaaatgg atatcaagat tcaagggatt actgcagaaa
    12181 tcttgacgga ggctcttgct caagccaaga aagcgcgttt tgaaatcctt gatgtcattg
    12241 aagcaaccat tccagaagtt cgtccagaat tggctccaac tgctccgaaa attgatacga
    12301 tcaagattga tgtggacaag attaagattg tcatcggtaa gggtggagaa accatcgaca
    12361 agattatcgc tgaaacaggt gttaagattg atatagacga agaaggaaat gtgtctatct
    12421 actctagtga ccaagatgct attaaccgtg ccaaagaaat tattgctggt ttggttcgtg
    12481 aagccaaagt ggatgaagtt taccgtgcta aagtcgttcg tatcgagaaa tttggtgcct
    12541 ttgttaacct ctttgataag acagatgccc ttgttcatat ctctgagatg gcttggactc
    12601 gtaccaatcg tgtagaggat ttggtagaaa tcggggatga agttgatgtt aaggttatca
    12661 agattgatga aaaaggccgt atcgatgcct ctatgaaggc tcttctacct cgtccgccaa
    12721 aacctgagca tgatgaaaaa ggtgaaaagt ctgagcgccc tcaccgccca cgtcatcaca
    12781 aggatcacaa acctaagaaa gaatttacag aaacaccaaa agattcagag taagaaaagg
    12841 agaaatgtat ggggtggtgg cacgaaacca ttgatatcgt aaaagaaaat gatccagcgg
    12901 cccgcaccac tttggaggtt ttgctgactt atccaggtgt caaggccttg gcggcccacc
    12961 gtctctcgca ttttctctgg aagtacgact tcaaactctt agctcgtatg cacagtcagt
    13021 tttggcgctt ttggactcag attgagattc atccaggagc ccagattgat tcaggtgtct
    13081 ttattgacca tggttctggt ctggtgattg gagagacagc gattgttgaa aaaggcgttc
    13141 ttctctatca cggagtgact ctcgggggaa cagggaaaga ctgtggcaaa cgccatccga
    13201 ctgtacgaaa gggagccctc atatcagccc atgcccaagt tatcgggcct gtggaaattg
    13261 gtgaaaatgc caaggtcggt gctgcagcag ttgtcgtagc agatgtacct agtgatgtga
    13321 cggttgtcgg tattccggcc aagattgtcc gtcttcatgg taagaaagat gagcctgtta
    13381 ttcacgaagt cgaagaaaaa cgggagtatt atgtcaataa actcgagcag gctaaagatg
    13441 ccagtcacag atcgtctggt ttgtagagga tacctatatg attcaagcaa gaaacaagtt
    13501 aagccaagag gagctatctg aggcgaaaaa agtaattaac tgttgccaaa actatgacgg
    13561 tacctatcgc gatccctatc tctctaacat gcttaatttt gacccaaaca tgcccgcctt
    13621 tttcctttat tatgaaaaag gcaaacttgt tggtttatta actgtctatg cagatgacca
    13681 agatgtagaa gtgacgatac tggttcatcc aggtcatcgc cgtcagggga ttgcgcgtgc
    13741 attgtttact agttttgaga gagaaacagc ttctttcccc attcggtcag tcacttttca
    13801 gacagaacgt atttttttag agaatcatcc tgactttgcc agtaactggg gactaatcga
    13861 ggatgaagag acagaaacct ggttaggtaa ggatagaaga ccatatcagt tagcaaaact
    13921 ttctaatctt gaagttttgt tagcagatag ttcgtatcag gagcaaatta gccagttaaa
    13981 atttcaggca ttttcagggg aacatgaatc gagagaagtt gtggatagat atgtcgctga
    14041 agctctgaag gatccagaaa gtcgcttata tattttgtta aaagacggtc aggttattgg
    14101 gacttgcacg gttgatttat cgagcgatac gaattacttc tacggtttag caatatcaga
    14161 acctgaacgt ggaaaaggct atggaagcta cttagcaaaa tcccttgtca accaactaat
    14221 tgagcaaaat gataaggaat ttcagattgc agtggaagat agcaatgtag gtgccaaacg
    14281 tttgtatgaa aaaattggct ttgtcaaaca gacacaggtg gtttatctga atgagaaagg
    14341 agcaagggat tccgaagtgt agagacattc ggactgaaat ttacttgagc tcttagttta
    14401 gtgatgaaac taacagttct tggattttag ctttcttgat tatgatttat gattaaaatc
    14461 tatgacacca tgtctcgtga tttgcgagaa tttgtcccga ttgaggacgg caagatcaag
    14521 atgtatgttt gtgggccaac cgtgtacaac tatattcacg tgggaaacgc ccgttcgacg
    14581 gtagcttttg atacgattcg tcgctatttt gagtaccgtg ggtataaggt tgcctatatt
    14641 tccaatttta cagatgtgga tgataagatt atcaaccgtg ccagggaaga aggcatcaca
    14701 cctcaggagg ttgcggataa gtacatcgct gcctttcgtg aggatgtgac ggccttaggc
    14761 gtgaaacctg cgactcgcca tccgcgtgta gtggagttta tggcagacat catccgtttt
    14821 gtggaagact tgatcgagaa aggctttgcc tatgagagtc aaggggatgt ctatttccgt
    14881 gtagaaaaat cccacaacta tgctaaattg gctaataaaa ccttggaaga tttggagcta
    14941 ggtgcttcag gtcgtaccga tgaagaaacg gctcgtaagg aaaatcctgt agattttgct
    15001 ctatggaaat cttccaaacc aggtgagatt tcttgggata gtccttgggg acctggacgt
    15061 ccgggttggc atattgagtg ttcagtcatg tcgacagaga ttttaggtga taccattgat
    15121 atccacggtg gtggagctga cctagagttt ccacaccaca ccaatgaaat tgcccaatct
    15181 gaagctaaaa caggcaaggc ttttgctaac tactggatgc acaatggctt tgtcaatatc
    15241 gacaatgtca aaatgtctaa gtccttgggt aacttcatta cggtacacga tgcccttaaa
    15301 actcttgatg ggcaagtact tcgtttcttc tttgcgaccc agcactaccg taagcctatc
    15361 aacttcacgg aaaaggcagt tcgcgatgcc gagaccaatc tcaagtatct gaagaatact
    15421 tacgaacaac catttacagg aaacgtggat gcccaagagt tacaaaactt tgaagataag
    15481 tttgtagcag ctatggatga agattttaat gctgccaacg gtatcacagt tgtctttgaa
    15541 atggccaaat ggatcaactc agggaactat gatgcaagtg tcaagcaagc tcttgcagat
    15601 atgttagaaa tttttggaat tgtctttgtt gaggaagttt tggatgcaga gattgaagac
    15661 ttgattcaaa aacgccaaga ggcgcgtgcc aatcgagact ttgagacagc agaccaaatc
    15721 cgtgaccaat tggttactca aggaattaag ctccttgata ccaaggatgg agtgaggtgg
    15781 acacgtgatt gatgtcaatc tcattaacgg gattgcgcta gcctttgagg gggatgcggt
    15841 gtattctatg tatattcgcc gtcacctcat cctcaaaggt atgaccaaac ccaataaact
    15901 ccatcaagaa gcaaccaagt atgtgtcagc caaggctcag gctcgcctga ttgctctcat
    15961 gttggaggag caggtcctaa cggaaaaaga agaagaaatc tacaaacgtg gccgcaatac
    16021 caatagccac acaaaggcta aaaatgcaga tgtcgtgact tatcgtatgt ccacgggatt
    16081 tgaagcggtt atgggctatc tccatatgac tgagaatctg gaacgtcttg agagtttggt
    16141 ttcatggtgc atccaaaaag tggagggcta gaacatgagg gaaaaagaac tacaagactg
    16201 gtttcctgag gctcggattt cagaccaacc agtagagaaa gagggctatc tcacgctccc
    16261 tttagcttct cagcagtgga ttttgctgga ggaagctggg ctcagcgagc gtgaaaagca
    16321 gttggttgcc cttttgaccc agcaggagca ggctcgttcg ctaaaccctt ggtattccta
    16381 tctggttgaa ggcaagggac aggcaccgca agtttttaaa aagattcagt tggtttattg
    16441 ccatctttct tattttcagc aggaaaatct ggcttcttgg ctagatatga tgcggactct
    16501 ttttccgaat tgtcagacag tgctacaggt cggatctcag gattatgttt tcgtgcttca
    16561 acaagacaaa tacacttctg taagagatat tttaagtgat acgattgaag cggttgagta
    16621 tgactttgga cttcgtcttt ctatcatgtt gggtcaggtt tggtctcaga caggacatca
    16681 agccctatca gacttaatca aagctgagcg ggatttgttc aagacatggt ggcgtcaggg
    16741 tcaccaaggt gttcatactt tttctcagct ctatctttgg agtatgggag aaagactcgt
    16801 ggacttgaag ccaattaaag aatgtctaca ccagatgatt ttggatcaag atcagattca
    16861 ggaaatcatt ctctctcttt gggaaaatag tgctgttctc actaaaacag cccagcaact
    16921 ctatctgcac cgcaattctc tccaatacaa gattgataaa tgggaagagt tgacagggct
    16981 tcagttgaaa gagttgaccg acctgacctt gtgttatcaa ttgattttag gttctttgtc
    17041 aactatagtt ggtttgtaaa gaagttaata tttggagaag aggatggcca tcttctccat
    17101 ttttatgtgc agagttatag tggattgatg ctgggaaagt acactgtgac tgctaaaaca
    17161 ttcctagaag ttgctttgat ttccctaatc tatttatgca aattttatgt tattttacta
    17221 taacagttgt tgctaagcca aataatagtg gggaagttca tttagacgta agcattgaag
    17281 ataatcaggg aggtagtggg cataatttca gttctgtttc aagtagctca caaacagcta
    17341 aatatgaaga aactggttat aataacaatt tatcattata tataacgatt gataaaacgt
    17401 ctgatgcaac agctcttttg aaattaaagt tgaataatgt tgataatcaa cctgctactg
    17461 aagttcctag ttcaggaatt actgtaaaat taaatactaa agataatgct ggaaactgga
    17521 caagtgcttc gaataaaaaa gaaataacag taaaaattgt ttctgctaaa ccgacatatc
    17581 cagacaaaat cttagtgaaa aatcctgata atataaaaga tacagaaaaa aatgccatta
    17641 ttgaaaaatt gaaagaggca aataaaaatc atccagcagg agctccaacc tttgctaaag
    17701 gtgaaggaga gcatgcaaat gatattgtag caacttattc agatggtaca acttattatg
    17761 taccgttaaa tgatgtgaca aaatatgcga ggtagtggct gtaccactca cttattcacc
    17821 tcctcgtgat ttgtagtagt gataggcttt ctcactatta ttataaaaca aaataaagat
    17881 cacaacactt tttcattctg tgttgtgcct tgagtgaaac gaaaggaatg aattataaat
    17941 atgaaaagta tagtcac
//
LOCUS       DAVLYV010000034        17795 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLYV010000034 DAVLYV010000000
VERSION     DAVLYV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.034"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..224
                     /locus_tag="VYO70_001164"
     CDS             <1..224
                     /locus_tag="VYO70_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1713402.1"
                     /translation="FQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFG
                     FRNFENFKKQIFIALNIKKERTKFVLSRA"
     gene            complement(486..1460)
                     /gene="pta"
                     /locus_tag="VYO70_001165"
     CDS             complement(486..1460)
                     /gene="pta"
                     /locus_tag="VYO70_001165"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1713403.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(1504..2400)
                     /locus_tag="VYO70_001166"
     CDS             complement(1504..2400)
                     /locus_tag="VYO70_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1713404.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIYIPAEEGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            complement(2397..3215)
                     /locus_tag="VYO70_001167"
     CDS             complement(2397..3215)
                     /locus_tag="VYO70_001167"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1713405.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            complement(3199..3870)
                     /locus_tag="VYO70_001168"
     CDS             complement(3199..3870)
                     /locus_tag="VYO70_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1713406.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            3998..4567
                     /locus_tag="VYO70_001169"
     CDS             3998..4567
                     /locus_tag="VYO70_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1713407.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            4680..5639
                     /locus_tag="VYO70_001170"
     CDS             4680..5639
                     /locus_tag="VYO70_001170"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1713408.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            5649..6764
                     /locus_tag="VYO70_001171"
     CDS             5649..6764
                     /locus_tag="VYO70_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1713409.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            6769..7116
                     /locus_tag="VYO70_001172"
     CDS             6769..7116
                     /locus_tag="VYO70_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1713410.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            7124..7339
                     /locus_tag="VYO70_001173"
     CDS             7124..7339
                     /locus_tag="VYO70_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1713411.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            7422..8063
                     /locus_tag="VYO70_001174"
     CDS             7422..8063
                     /locus_tag="VYO70_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1713412.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            8081..8770
                     /locus_tag="VYO70_001175"
     CDS             8081..8770
                     /locus_tag="VYO70_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1713413.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPEFLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(8767..9447)
                     /gene="radC"
                     /locus_tag="VYO70_001176"
     CDS             complement(8767..9447)
                     /gene="radC"
                     /locus_tag="VYO70_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1713414.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9490..11781)
                     /gene="pcrA"
                     /locus_tag="VYO70_001177"
     CDS             complement(9490..11781)
                     /gene="pcrA"
                     /locus_tag="VYO70_001177"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1713415.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYCADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGPGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVAEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASIEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            complement(11973..12778)
                     /locus_tag="VYO70_001178"
                     /pseudo
     CDS             complement(11973..12778)
                     /locus_tag="VYO70_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(13158..14018)
                     /locus_tag="VYO70_001179"
     CDS             complement(13158..14018)
                     /locus_tag="VYO70_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1713416.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            complement(14034..15311)
                     /locus_tag="VYO70_001180"
     CDS             complement(14034..15311)
                     /locus_tag="VYO70_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1713417.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            complement(15304..15855)
                     /locus_tag="VYO70_001181"
     CDS             complement(15304..15855)
                     /locus_tag="VYO70_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713418.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            complement(15871..17130)
                     /locus_tag="VYO70_001182"
     CDS             complement(15871..17130)
                     /locus_tag="VYO70_001182"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1713419.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
BASE COUNT         5388 a         3874 c         3252 g         5281 t
ORIGIN      
        1 tttttcagac tgtctttaaa accttcctca aagataaaga aaagattgtc aacgcccttc
       61 aactacacta ttctaatgcc aaactggaag cgaccaataa tctcatcaaa cttatcaagc
      121 gcaatgcctt tggttttcga aactttgaaa acttcaaaaa acagattttt atcgctttga
      181 acatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagcttttc ttcaacccac
      241 tacagttgac aaagagccaa aaaattctct tttcatactc atttaaaatc aaaaagggaa
      301 tttatcgttt tacggatatg tatattacag atttctatag gtcgtgtata gacaggatat
      361 catcgcttgg aagttttcct ttttgacacc aatcagtaat ttagttctca ggaattaatg
      421 gcagtgcttt tcgtacatag tttcataact acagtatagc atagtatatc actttatagt
      481 tttcactatt gatgaactgc ttgagctgct gtgatgaggg tcaacttgta aacatcatct
      541 gcattacatc cacgagaaag atcattaact ggcttgttta aaccttgcaa aacaggtcct
      601 acagccgcaa agccacccag gcgttcagcc atcttgtaac cgatatttcc tgcctcgata
      661 cctgggaaga tgaagacatt tgcttgacca gctactgtac ttcccggagc tttcagagct
      721 gcagtttcgg gaacaaaggc cgcatcaaat tgcaactcac catcgatttc aaggtcagga
      781 cgcaagtcgt gagcaatttt agttgcttca acgaccttat caacgctttc accaaaccct
      841 gaacctttag tagaatagct caacatggca attttaggtt cgatgccaaa catcttagct
      901 gtgattgctg agttgatggc aatttcagcc aaggcttctg catctggatt gatattaatg
      961 gcacagtctc caaataggta acgttccgta ccacgaacca tgaggaaggc tcctgaagta
     1021 cgagttacat ttggacgagt tttgatgatt tgtagagctg ggcgaactgt tgaagctgtt
     1081 gagtgaatcg ctcctgacac cattccatca accaagccca agtaaaccaa catcacacca
     1141 aagtagttga catcttcaac caaaaccttg cgtacatctt cttcagtcat tttgcccttg
     1201 cgacgctcca ccaaggcaga aaccatttct tcaaattgag gataatgttg agggtcgatg
     1261 acctcataac catccatgat tccttcaatt tcaagataaa ttttaatttt ttcaggattt
     1321 ccaagcaaaa caggaatcac ttctgtttct tttactaagc gttttgttgc ttgaagaata
     1381 cgaggctctt ccccttcagg gagaacgata cgagcatttt taccaacaag gttggctttg
     1441 agactttcaa aaacttccat gagttttctc ctttaagata ataattatac tctgagacag
     1501 tttttataga gtattagttg ataactgggt aataaggtta ctgaaatcat ccggcaaggg
     1561 actttctaac tgcaagtctt gctctaaaaa tggatgataa aaggataggt aatggcaatg
     1621 cagagcctga cgttgaatac catcttccag actaccacca tacaaatcat ctcccagcaa
     1681 aggaaaaccg atatgagaaa aatggactcg gatttgatgg gttcgaccag tgtgcaggtg
     1741 aatatagacc aagtgaatat ttccataaga agctacaatc ttgtatgaag tatgggcata
     1801 ctttccgcct ttagccactc gtctggtaat aatggaatct tcatcacgcg caatcggagc
     1861 aataatttcc ccttctggct ccaaatgtcc atctccctta accaaagcaa agtagcgttt
     1921 ctcgatagat ttcttctgca actgcttgtc taatcgtgca tgggcataac cgtgcttggc
     1981 aaagagcatc aagccagaag tgtccctatc tagtctggta acaatgtgaa cctgctgatt
     2041 ttcataattt tgcttgacat agtaaccctt gataaaattg gcaatggtat tagagtgatt
     2101 gacactagga atagaagcca ctccataggg tttattcaag actagaaagt ggtcatcctc
     2161 atagagaata tctaatggaa gctcaatagc ctccaaggtt tcaaagcctt cctcagcggg
     2221 aatgtaaatg gtaacgtagt ctccaacgtc caatagatac gttgcatttt gcggttgatt
     2281 attgaccaga atagctccac ctcgaaactt aatcttagcc agcaatccct tagaaacctc
     2341 gtgctttttt aagaaggtct taaccttgac atgttcatct gcgataaatt caaacctcat
     2401 tcatccacct caccgataaa ggcatcctta acacggttcc agaaactggt atggctagga
     2461 gtcgcgacaa agtgaatctt atgatggtcg atttgatact caatacgctc aatattacgg
     2521 aaagaataaa cgctattgtc aaccgaaata gtatgataat cgtttcttgt tggaataagt
     2581 tcaatcttat ccttcttagg cacaataatg gaagagccca gtgttcgata aacacgatta
     2641 ttaaggctgg caatttccgt taattgcaaa gcttcaatgg tagggtgtaa aacagcaccg
     2701 ccaagagact tgttataggc agtactacca gtcggtgtcg aaactgttag cccgtctcca
     2761 cgaaaacgtt caaagggaac accatttatt acaatatctg ccaccatggt tcgatcagac
     2821 ctgcggatgc tggcttcgtt gagtgctctg aaaatcttaa cttcaccatt ttcaagaaag
     2881 accttcacat tcagaacagg gtaagaaacc cttgccccag tatctagctg caaattagtc
     2941 actagcttgt ccaactcaaa atcacgataa tctgtataga agcccaaatg tccagtatga
     3001 agaccgataa agcggacctt gtcaagctga ttttcgtact tatgaaaggc cgacaagagc
     3061 ataccatccc cgccaatgga aatgacaata tccggattgg tatcattgag tataaactga
     3121 tttctcttca aacgatctcg caattcatac aaaacccttt gactctgcgg ttttctattg
     3181 gctatcagat caattcgttt acctgtattc ttcatctgta tcgtcactgt ttcctacacc
     3241 gtcatttaat tttctactca aaggatcaaa aagtgcctgg gcttcttgga tatcatcacg
     3301 aatttcaccc atttcttcat ccaactgatg ggcgattcta gctgtaattt ccagtcgctt
     3361 cttaatctca tctgggaaat ccccttggta cttgtagttg agagaatgtt ctatcgttgc
     3421 ccagaaattc atggccaaag tacgaatttg aatttctgcc aaaatagtct tagctccatt
     3481 gatggtatca accgtatatt ctactaccac atgataggaa cgatagcctg atgcttttct
     3541 atgagtaatg taatctcgct cctgtatgat tcgcatatcc tgacgcttgt gcaaaatatc
     3601 cactacttcc ttgacgtcat ctacaaactg aaccatcaca cgtaagccag caatatcctg
     3661 caaatcgtgt tccaaggtcg cataagtaat gccacgacga gccatttttt ctttgatgct
     3721 ctcaattggc ttaactcgac cggtcacaaa ctcaattgga gaatgcttat tttgcttacg
     3781 atattgctta cgaataccac gaagtttaat ctttaactca ccaacagctt gaatgtaagg
     3841 atctagaaat tcttcccatt ctaaggtcat atattctccc ttgttctcat attatccgtc
     3901 aaaaatatct ttgatttttt ctccagattc atatacaata aatacaatgc ttattatacc
     3961 ataaatccgc tttcaacgat aaaaaaaggt aagaaaaatg aaacatttag aaattgaatt
     4021 gaaaacacta ttgaaaaaag atgaatacaa tcgtctaaaa gaccagttca caggtgtcac
     4081 tcctgttctt caaacaaatt actacatcga cacgcctgat tttgaactgc gagaaaagaa
     4141 agtcgctatg cgcattcgaa cctttgaaga ctgggctgaa ttgacactca aagtcccgca
     4201 gagtgttgga aacatggaat acaaccaaaa attacaacta aaagatgctg agaactatct
     4261 gagtaaggaa gaacttcctc aagggctagt actggatgaa ttggcgaaac atggtatcca
     4321 aagtaagaac tggcaggttc ttggttgtct aacaacgctt cgctatgaaa tgaaaacagc
     4381 tattggtctc atggcactgg atcagagccg atactttgat ataacagatt acgaattgga
     4441 gcttgaagtg gaaaatcatg agcaaggcaa acaggatttc cgacaatttt tagagaaaaa
     4501 tcagatttcc taccaaaaag ctccttcaaa attggttcga tttgtcaaaa gtatgaaaaa
     4561 tagctgaaat aatctccatt ttttggtaaa atagaaaaga taaaaataca caaatcccag
     4621 ttcatatatg taaggtaaat ataactagga tttataaagt ttacagagga cggtctataa
     4681 tgtcagatag aaaaaacatg aaacttttcg cactcaactc taaccaagag attgcacaaa
     4741 aaattgccca agctgttggt gtcccacttg gaaaactatc atcacgtcaa ttttcagacg
     4801 gagaaatcca agtaaatatc gaagaaagtg tccgtggtta tgatgtttac atcatccaat
     4861 caacaagttt ccctgtcaac aaccacctaa tggaattgtt aatcatggtc gatgcttgtg
     4921 tgcgtgcaag tgcccacagt atcaacgttg tccttccata ttttggctat gcacgtcaag
     4981 accgcattgc ttgtcctcgt gagccactta cagcaaaact agttgccaat atgctggtta
     5041 aggctggagt tgatcgtatc ctgactcttg atttgcatgc cgttcaggtt caaggtttct
     5101 ttgatattcc agtggataat cttttcactg ttcccctatt cgcaaaacat tactgcgata
     5161 aaggattgct tggttcagat gttgttgtcg ttagccctaa aaattcaggt gtcaaacgtg
     5221 cgcgtagcct ggctgaatat cttgatgctc ctatcgccat tatcgactac cctcaagacg
     5281 atgcaactcg taacgaaggt tatattattg gtgatgttga aggtaagaaa gctatcttga
     5341 ttgatgatat tttaaataca ggacgtacct tctctgaagc ttctaaaatc gttgaacgtg
     5401 aaggagctac agaaatttat gctgtttcta gccacggtct cttcgtcgag ggagctgctg
     5461 agcttcttga caatactaat attaaagaaa ttcttgtgac tgattcagta gcaacaaaag
     5521 aaaaaactcc taaaaatgta tgctacatca ctgctagtga gttaattggt gatgctatcg
     5581 tccgtattca cgaaagaaaa ccagtcagcc cactctttgc ctacaataaa aagaaataag
     5641 gtgattcttt gatttatttg gacaatgctg caacgactcc tatgtcagca gttgctattt
     5701 cagctatgac caaggttatg caagaaaccc acggaaatcc ttctagtatt catggtcatg
     5761 gtcgtcaagc tggcaaactc ttgcgagaag cccgtcagga actagcccag ttactaagga
     5821 caaaacctca acatatcttt ttcacttctg gtgggactga aggcaataat actaccatca
     5881 ttggctactg tcttcgtcac caagaacaag gaaaacatat catcacaact gccatcgagc
     5941 accatgctgt ccttgaaaca attgattact tggttcaaca ctttgggttt gaagcaacca
     6001 ttatccagcc agaaaatcaa gaaatcacag cccagcaaat tcaaaaggct ttacgtgacg
     6061 atacgatttt ggtttctacc atgtttgcca ataatgagac aggaaaccta ctgcccatcg
     6121 ctgaaattgg ccaaatactc aagcaacacc ctgctgccta tcatgttgat gcagttcagg
     6181 ctattggtaa aatcccaatt cattcagaag aattgggcat tgattttctc actgcttctg
     6241 cccacaaatt ccatggtcct aagggaatcg gttttctcta cgcatctagc atggactttg
     6301 attcctatct acatggcgga gaccaggaac agaaaaaacg tgcaggaact gaaaatctgc
     6361 ctgctatcgt aggcatggtt gcagccctaa aagaagacct agaaaaacaa gaagaacatt
     6421 ttcaacatgt acaaaatcta gaaactgcct ttctggcaga gctggagggc attcagtatt
     6481 acttgaatag aggaaaacat catctccctt atgttctcaa tattggattt cctggtcaga
     6541 aaaatgacct cttactcctt cggctagatt tagctggaat ttcaatctct actggctcag
     6601 cctgtactgc aggcgttgtc caatccagcc atgttcttga agccatgtat ggcgcaaatt
     6661 cagaacgctt gaaggaatcc cttcgcatca gtttgtcgcc acaaaatacc gttgaagacc
     6721 tacaaaccct cgcaaaaacc ttaaaagaaa ttatcggagg ttagccatat ggcatttgaa
     6781 aaaatcattc agttaaaaaa ttgtcgttac gattacactc ttagcccttc tgttaaaaaa
     6841 ttcaccctca aagataacac cttttttgaa actaaggttg gtaactatga actgactcgc
     6901 cttttggaaa aagtgccaaa cagcggtgaa ggcttccaac tcaaaatcat cattaacaag
     6961 gaacttacag gggctaaaat caatatcact gacaagtttg gccttcgtct agttgatatt
     7021 ttcaaatcag aagaccacca tattcatcag gaaaaattct acttcctcat ggatagcttg
     7081 gtagaacgtg gtgtctttac aaaatcggaa agatagagcc aaaatgtttg aactgaccta
     7141 taaggactgc tatcatgtag agcgtactct caagtatgag gatcatgagg ctctcatgtt
     7201 gactttgtca ggatgtgtaa ccttaccaga tacactttat gtgacttctc tgacttttag
     7261 gggcaaaaaa gtttatcagg ggctggtcgg agacctctac cgttttctat ctcatgcaga
     7321 ttttttacat caaaactaag atttttctta gttttttctt atttttgttg attttttcac
     7381 aatgtttcta taaaatagaa gaatagaaag gttgtgattt tgtgaaagat aaacagtttg
     7441 ctattccaaa agctacagca aaaagactct ctctctacta tcgaattttt aagagatttc
     7501 atgcagaaaa gattgaacgt gccaactcta agcaaattgc agaggccatt gggattgatt
     7561 cagcgaccgt acgtcgtgat ttttcctatt ttggtgaact tggtcgtcgt ggttttggct
     7621 atgatgtcaa aaaactaatg acattttttg ccgatttgct caatgacaac tctattacca
     7681 atgtcatgct ggttggtatt ggaaatatgg gccatgccct tctccactac cgcttccacg
     7741 aacgtaacaa gatgaagatt atcatggcct ttgacctaga cgaccatcct gaagtcggta
     7801 cccaaactcc tgacgggatt cccatttacg ggatttctca gatcaaggat aaaatcaagg
     7861 atgctgatgt gaagactgct atcctaactg ttcccagcgt caagtcacaa gaggttgcta
     7921 atctcttggt tgatgctggt gtgaaaggaa ttctcagttt ttcaccagtc catctgcatt
     7981 taccaaaaga cgtggtcgtt cagtatgtcg atttgacaag tgaactccaa accctcctct
     8041 acttcatgcg aaaagaggat tagaaagcga aaatcatttt atgaaaaaac cagttattgg
     8101 gattacagga aacgaaaaaa ctcatccaga tgatgacatc atgatgagct acgcagcaaa
     8161 aggctttgtt gaaggcgtta aagatgctgg agggattcct atcatcctac cgattggtga
     8221 tcaagaaatg gcctgccact atatcagttt gattgacaag ctcatcttga caggtgggca
     8281 aaatgtcgat ccaaaattct atggcgaacc caaaaccatt gatagcgatg attatcacct
     8341 tcaaagagat atcttcgaac tggccctcat caaggaagct attaaacaga aaaagcccat
     8401 tttctctgtc tgccgtggta cccaactctt taacgttgcc atgggtggaa ctttgtacca
     8461 agatatcgaa gaccattggc aggattcttc tgttgagtac acaactcaac gcttggtgac
     8521 agaaccagat accgttctcc aagaaatcta tggagaaatc tctcatatca actccttcca
     8581 ccaccagagc atcaaggact tagcaccaaa tttaaaagtt gtggctcatg atcctaaaga
     8641 tggtatcatt gaggctgtca tgagtacgga tgatgtcgcc tttctcggtg tccaatggca
     8701 tccagaattt ctttttgaaa atcgtcccaa agataagaaa ctctttgact atgtcgttaa
     8761 tgaactttag attaaatctg tcttttcacg ataactaaag taattagaat gagagacaat
     8821 caaatggtcc aagagaacaa tccccatcag ttcgcaggct tctttaacaa gtttagtgac
     8881 atgatcatca ttttggctag gcgctaccgc tcctgaagga tgattgtgga ccaagataag
     8941 agaagtcgcc atgtgcttga ttgcatagtg aagaatctct cgcggttcag cgatactacg
     9001 agttacagaa ccgataaaaa tggtctgctg atggatgatt tgattttgag tattgagata
     9061 gagtgccacc aggtgctctt gttttttatc ccctaattcc tgctgcatct tcttggccaa
     9121 cttttgactg ctgagaatac tttccatttc aagagtctcg tgtttgtgaa tacgatgccc
     9181 cagttcaatc atagcttgta attctatggc cttaacacgc ccaataccag acaaactctg
     9241 caattcctgc agggtcattt ttttcaaatc cgttaggctt gaaagattgt tcaagacttt
     9301 ttgggcaatt tcaaaaacgc tagcttgacg tgttcctgtc ctgagtaaaa tagctagcaa
     9361 ctcttggtta ctaagcgctt caactccttc cttggccagc ctttctcttg gtaatagtga
     9421 atcttcttgg aatgaaatac tgtacataaa aatcctcctc actttattat tcgtgagaag
     9481 gatggaaaat tagatttttt tctcaattgg agccacactg gctaaaagtt ttttcaaacc
     9541 tacttctggg aaattgattt tcaattcctg cctagcaccg ctacctgaaa cttccagaac
     9601 ggttccctct ccccatttct tgtggagagc aatgtcacca atggaccaat ttgcctcgat
     9661 agatgctgat tttgcgccag ctgtaaattg accaaatgga agaccgcttg actggattga
     9721 ttttggggca gcaccgcgtt tacggtcttg aagagcctga gccaaactca taccttgacc
     9781 aaaggaaata ctaccactgc tatatgatgc cttaaagctt gtatttgcag gacgagccag
     9841 accttgatac tcaagcaagt ctgaactgat ttcgttaata aaacgagtcg gacggttata
     9901 attggtacga ccaaaaagca agcgtgagtt ggcattggtc agatagagaa ttttctctgc
     9961 acgcgtgata cctacatagg ctagacggcg ctcttcttct aattcatctg gatcttcagt
    10021 cgcacgacta agtggaaaga cattttcttc catcccaatc aaaaagacaa ctggaaattc
    10081 gagaccttta gcagcatgca gggtcatcaa ggtcacttct gatgtctcct gactacctga
    10141 atctgtgtcg gcaatcaaag ccaagtcatt taagaaacga ctcagtttgt ccagaccagt
    10201 ttcctcttct gccacatcag tggtgtcatc aaagttcttc gtaacagaaa gaaactcttc
    10261 gatattttca acccgagcct tgctttctag agtcgcttgg gcgttaagaa tatcgacata
    10321 acctgttttt tctaggacgg actcaaccaa ctctgtaatg cttaagtggt ctagctgctc
    10381 ccgcaaatca agcatcatat tggcaaaatc ccagatagat tgggctgcct tacccttgat
    10441 accagacaac ataatattag cagaagcatc cagcatagac atattttgca aatttgcaaa
    10501 atcacggatt ttctcaactg tacctggacc aattccacgt ttaggctcgt tgataatacg
    10561 ctcaaaacta atattgtcac tcaaattagc aataaggttg agataagcaa taatatcgcg
    10621 aatttcctta cggctgtaga atttggttcc gccaaccatg gtataaggaa tgttagactt
    10681 gagcagggct tcctcaattg tacgggactg ggcattagtc cgatagagaa ctgcaaaatc
    10741 cttatgaagg aagttttgac tgcgactaag ttcatcgatg gttctggcta caaatacagc
    10801 ctcatccagc tcatcatcgg cacaatagta aacgatttgc tccccatcag cgttttgagt
    10861 ccagagattt ttaggacggc gatttttatt gtttttaata acctcgttgg ccgcttggag
    10921 aatggttttg gttgagcgat aattttcttc caacaaaaca accttggctt tggggtaatc
    10981 cttttcaaag tccaagatat tctgcatatc agcaccacgc caaccgtaga tagactggtc
    11041 cgcatcccca accacacaga tatttttaaa acgggaagcc aagagtttga ccaattggta
    11101 ctgagcgtgg ttggtatctt ggtactcatc aacgtggatg tattggaatt tttgctggta
    11161 gtaggtcaaa acatcaggat tttgatcaaa gagacgcaag gtcagcataa tcaaatcatc
    11221 aaagtcaacg gattcagact gacgaagttc tttttgatag gttgtataac actgggccac
    11281 aatttgcgta tacatatcgc cagcttgggc agcataagca acatcatcaa tcaaatcatt
    11341 cttagcattg gaaatggtcc ccaaaatagt tcgttcattc cattttttag gatccaagtt
    11401 caactgtttg agaatacgtt tcatgagcgt tcgctgttca ccaggatcca caattgtaaa
    11461 attacgattg tagccaatat ggtccgcatc gcgacgcaaa atacgcacac acatggagtg
    11521 gaaggtcgca atcagacagt cctgagtagc tggattgagg ctataagcac gctctttcat
    11581 ctcacgcgca gccttgttgg taaaggtaat ggccaagata ttccaaggat tgaccagctt
    11641 ttcatcaatc aaataagcga tacggtgggt taaaacacga gtctttccag aaccagctcc
    11701 tgccatgatt agcaagggac cttctgtcgt ttgcaccgcc tcagcctgac ggtcattcat
    11761 tccatttaat aatgcgttca tcttctcttc cttactcttg aagtcaatat ttctattata
    11821 tcagaaatca gggaaaatag aaactttacg gggagtagca aaatatagtg gattgaaact
    11881 agaatactac actgtgggtt tctaaaacac tcgacttttt cacccaattt tttcataaac
    11941 tagctcaata cctcaataga cttcctgcaa aactagaatc ctagttcatg attgataatg
    12001 ccagcaatca aattcgttcg taatccgaag cgtttacgat gatttcgata ggttgttgaa
    12061 aacattttaa acgtttttac tttggcaagg atgctctcaa ccttgcttct ctccttagat
    12121 aacgcatggt tataggcttt atctttaact gttagcggtt tgagtttgct ggatttacgt
    12181 ggagtttgtg cttgaggata tatcttcatg agcccttgat aattactgtc agccaaggtt
    12241 ttaccagttt gtccgatatt tctgcgactc attttgaaca acttcatatc gtggcaatag
    12301 ttcacagtga tatccaaaga aacaattctc ccttgacttg tgacaatcgc ttgagccttc
    12361 atagcgtgaa atttcttttt accagaatga ttggctagtt gattttttta ggacgattta
    12421 tttttacctc tgttgcatcc acaatcaccg tatcctcagc actaagatga gtttttgaaa
    12481 tcgtaacacc actttgaaca agagttactt caacccattg gctccgacgg agtaagttgc
    12541 tttcgtgaat accaagatca gccgcaattt cttcataagt gcggtattct cacacatatt
    12601 gaagagtggc cataagaagg tcttctaggc ttaatttagg ttttcgtcca ccttttgcgt
    12661 gtttaagttg ataagctgtt tttaatacag ctaacatctc ttcaaaagtg gtacgctgaa
    12721 caccaacaag acgcttaaat cgtgcatcag ttagttgttt acttgcttca tcattcatag
    12781 aactactata ccatattttg tttcgcaggg agtctaatat aaaaaaataa atttaagatt
    12841 ggcaatacga acgttaaaca gaattccatt tctcttttta gaatcaaaac gacaatattg
    12901 tggaatctct acaggaacaa tagactgcga cactaaaata tcctccctta gttttacttt
    12961 actaatagta tataggagga gagccctgaa tcctagaccc tttgttatga tgcgcttaca
    13021 aacagagttg gaaaaaatct taaaagcaga aaaacgcatg atatcagccc ctaacacttg
    13081 atacaatgcg ttttattatg aaaaaatcta ctggattttt tatcaaaata aacttccagt
    13141 agtcttttca ctttttatta ataagttcct tcttcacctt ggctagtcaa gataacaggt
    13201 ccatctttcg taatgacaaa ttggtgttca tactgacatg acaatccacc gtcaatggtc
    13261 ttatgcgccc aaccagtttt catatctgta tcaatttccc aatcgcctgt attgatcatt
    13321 ggttcaatgg ttaagaccat tccttcacga agacggagtc cacgacctgc aataccatag
    13381 ttaggaacca ttggttcttc gtgcatagtt gggccaacac catgaccaac caaatcacgc
    13441 actacaccgt aaccacgact ttcagcgtat tcttgaatag ccgcaccgat atcaccgata
    13501 cgatttccaa caacagcttg ctcaataccc ttgtacatag cttctttggt tacatccatc
    13561 aagtttttga cttcttcgga cggtgtacca acagcataag cccaacatga gtctgctaaa
    13621 ccaccagaat agctctgagt gtattttttc atttgttcaa cattgttgaa gtttaatttt
    13681 gagacattta ggtcagattt agcaatggga cctcccaaaa ccatatcaac tttgagcaaa
    13741 tcaccatctt tcaagatata atgacgaggg aaagcgtgag ccacttcatc gttaagagag
    13801 caacaggtag cataaggata gtccatcatg gcaccgtcaa ccccaatctg aagtggaagg
    13861 aaattttctt ctttacaacg acggcggaca tattcttcaa cttcccacat atctacgcct
    13921 ggcttaatca aatcacgtaa gcctatatga atacttgcta gaaaatcacc agccttgtcc
    13981 atagcttcga tttcacgagc tgattttaat gttatcatct tttctcctag tttctaatta
    14041 attttaacag tcacatttgc ttttgaaaca atctggtgac catgataaat atcgtaatca
    14101 attatagctg accgtctcgt atgatgaata atccgtgcct gaatgcgcaa tatatcatct
    14161 atctgaacgg cctgcaaaaa gtagatcagc atctgctcga taatgagatt gcgaccacta
    14221 ttaacaacta aatcttgggt catgtgggtc agaatttctg ccaatacacc attagccaaa
    14281 actccatttt tttctagcat aaagggttcc actgtaatga ctacttcatc atggtgataa
    14341 gagagctttt gtccaatctg ctcagaaaaa gttggtagag ccgaaacttg ggaacggctc
    14401 atcttctcca tgacatctcg tcgcgtcaca acgccaagca aagtttgatt gcttcgaaca
    14461 actggtacca tttcaaagtc ttctgcgatc atccgttgac tcacattggc aatatttgtc
    14521 gataatccaa ccaaaaatag actacgagac ataaccttat caattgtcgt gcttggtgat
    14581 ttatcaccag cgtctctcat ggttacaacg ccaacaacga cctgatgttg attgataaca
    14641 gggaaacggc tgctacgatt cttacgaacc aagtccaaat aatctttaac tgtatctgtc
    14701 tctctcagaa aaccatactc atgactaggg cgataaagtt tctcaactgt cagaatatca
    14761 gtcttgattt ggacatttga caaggcttta ttgatcatgg tagcgacggt aaaggtatca
    14821 tgcttacttc ttagaacagg aatccctttt tgattggcca gtttaagcac atcatcatga
    14881 acctgaaatc cccctgtaac cagaactgca ttttcatttt ccaaggctag caactgaata
    14941 cgggttcggt ctccgacaat caagagcccc ccatcatgaa ggtaagacaa gatattttgt
    15001 tcagtcatgg caccaattga aaacttacta aattctctct ctaaaccttc ttgcccagcc
    15061 agaacctcag aagaagtcac ttctgcaatt tcagcaaacg ttaatctatc tatagcaact
    15121 ttctgggatt taacacgaat tgttccactt ctaggacggg tctccacaat tccacggttt
    15181 tcagcttctt taatagctcg ataggctgtt ccatcactaa ctcctagatg attcgaaatg
    15241 ctacgaacac tgaccctttt acctactggt aattcctcca aatagcttag aatttcctga
    15301 tgcttactca ttgaactctc cttttacata ccgaaattca agataatccc gcatttttct
    15361 tgtgtaacga tcacttccct taaactgacg gaacaaacta aatccagact taagagcaac
    15421 tctttggcta gcttcatttt caaggtgggt aatgataaat aattgtttta agccaaattc
    15481 ctcaaaagaa agctgacaaa tttttctaac aacctctgtc ataaatcctt gcgaccaagc
    15541 atcttttctc aaaaaatagc caagctcagc ttcttttttg atttcatcta acttctcaaa
    15601 tttaatagaa ccaatcattt gttgattttt ctggtcacaa attgcccaca ctcccaaagg
    15661 ggacttcata aagtaattgg ccagtgcata ttgactttct tccagacttg cctgcgttgg
    15721 gaaaataaat tgaagatttt ctggatttga agctatctcg cggaagtcct gactatcact
    15781 aaaaaagaaa ggacgcaaat acaagcgatc cgtttcaaaa aaagaaaaca ttgctaattt
    15841 ggtccaaata ttcataaaca cctctacggt ttaatcctca acaagtctaa tatccgctcc
    15901 taaattacgt aatttttcga taatatcaga ataaccacgt aagataaact cgatattggt
    15961 aatttcagtt ttaccttcag ccataagccc agcaatgact agtgcagccc cagctcttaa
    16021 gtcggtcgct ttaacactgg ccccacgtaa atcacgtcca cccgtgtaca aaatatgacc
    16081 atttgttgtc gaaatatccg catccatctt tgctagttca aaaacatgat ttacacgttt
    16141 ttcgtaaatc gtatcgacaa ttgtaccacg accattcgct cttagtaaaa gaggggtaag
    16201 cggttgttgc aaatcagttg caaagcctgg gtaaggagct gtcttaatat tgattgcttt
    16261 caaattagac tgttcctcga caaaaatgct gtcttcagat acagtcattc tcactcccat
    16321 ttcttccaac ttagcaataa acccttccag gtgttcgtaa agaacattat ttatacgaat
    16381 tcctttacca actgcagcag ctaaagatat atatgttcca gcttcaatgc ggtctggaat
    16441 cacctgatga cgtgtcccat gtaatctttc aacaccatca ataatgatga tattagttcc
    16501 tgccccacgg atatgggcac ccatattatt caagagagta gctacatcaa taatctcagg
    16561 ttcacgggct gcattttcaa taatagtacg accatttgct ttaaccgcag caatcatcgt
    16621 attaatcgtt gctcccacac taaccgtatc catgtaaata cttgcaccat gaagtcctgt
    16681 atctttagca gataacttca tgttatctcc ctcgtagcta gcagtggcac ccatagcttc
    16741 aaacgcctta aggtgtaagt caatcggacg aggaccaaga tcacatcctc ccggtagacc
    16801 aactgtcgct tcaccaaaac ggcctaagag gctcccataa aaatagtaag atgcacgaag
    16861 actgttaatt ttaccataag gcattggaat attttgaaca cctcttgggt caatctccaa
    16921 tacatcgtca taacgcttaa cagtagctcc catcaattcc atgatttcga caagactggc
    16981 tacatccgaa atatctggaa cgcaatccaa agtcaccaca tcatcagcca agataatagc
    17041 tggaattaag gcaacgacac tatttttagc accactaata gtgatttcac cttgcagtgg
    17101 taatccacca ttgataacaa tttttctcat tctaagtttt caatactctt tcaagatttc
    17161 taataaggtc ttcaaaataa agtatatcat aaatttatcc ttttttccat ccttttcaat
    17221 tttattggat aaatcagagt tgacgagtta gtaactgaat tactaatact caatgaaaat
    17281 caaagatcaa actaggaaac tagccgcagg ttgctcaaaa cactgttttg aggttgtaga
    17341 tggaactgac gtagtcagta tcctacatat acggcaaggc gatattgacg tgatttgaag
    17401 agattttcga agagtataat ctttctcgaa tcgctattgc ccctttttta tcccctaaaa
    17461 ctgagtagga tctaagacta gatctcttat ctaagtatcg gaatacggct gttcaactaa
    17521 attttagcaa gaacttctcc ccttctctca gttcacagaa gcgttttata cataccgtcc
    17581 tatcagacaa tctattttaa agataaccgt gactcaggat cacttctatc tgtcgttttc
    17641 tctttatagt gatagacctc acgggataag tcgtacatct ttcctcgtgt atacaagata
    17701 taaggacgtt aaaaggttac agtgaaaata gaaaatctta gtacgaattc aattactctc
    17761 gtgatttacg catataggtt acgactacct ttttg
//
LOCUS       DAVLYV010000035        17279 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLYV010000035 DAVLYV010000000
VERSION     DAVLYV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17279
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.035"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            87..3437
                     /locus_tag="VYO70_001183"
     CDS             87..3437
                     /locus_tag="VYO70_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1713420.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3943..4413)
                     /locus_tag="VYO70_001184"
     CDS             complement(3943..4413)
                     /locus_tag="VYO70_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1713421.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4430..6703)
                     /locus_tag="VYO70_001185"
     CDS             complement(4430..6703)
                     /locus_tag="VYO70_001185"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1713422.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7050..10151
                     /locus_tag="VYO70_001186"
     CDS             7050..10151
                     /locus_tag="VYO70_001186"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1713423.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGQSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGVKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSNELEEKLNEIVMKT
                     IYR"
     gene            10233..11240
                     /gene="pfkA"
                     /locus_tag="VYO70_001187"
     CDS             10233..11240
                     /gene="pfkA"
                     /locus_tag="VYO70_001187"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1713424.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            11299..12804
                     /gene="pyk"
                     /locus_tag="VYO70_001188"
     CDS             11299..12804
                     /gene="pyk"
                     /locus_tag="VYO70_001188"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1713425.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(13079..13327)
                     /locus_tag="VYO70_001189"
     CDS             complement(13079..13327)
                     /locus_tag="VYO70_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1713426.1"
                     /translation="MQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHKNA
                     KTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDK"
     gene            complement(13494..13719)
                     /locus_tag="VYO70_001190"
                     /pseudo
     CDS             complement(13494..13719)
                     /locus_tag="VYO70_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            14567..15439
                     /locus_tag="VYO70_001191"
     CDS             14567..15439
                     /locus_tag="VYO70_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1713427.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHVTSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(15585..15929)
                     /locus_tag="VYO70_001192"
                     /pseudo
     CDS             complement(15585..15929)
                     /locus_tag="VYO70_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            16072..16383
                     /locus_tag="VYO70_001193"
     CDS             16072..16383
                     /locus_tag="VYO70_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713428.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            16387..17103
                     /locus_tag="VYO70_001194"
     CDS             16387..17103
                     /locus_tag="VYO70_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1713429.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
BASE COUNT         5360 a         2943 c         3686 g         5290 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgaaaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tggagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttacatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgaattt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gaacaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctacgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagataaag
     1381 aaggagctca atcaagtcgt atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagacg
     1801 atagctgtga aaaagatatt gatgggagtg tgtttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggataattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgttgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctctggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagccgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gcagatggaa
     3781 gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
     3841 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
     3901 accaggtttt ttaaaagcat aattgttagt tgtagtcatt tattattctt caaagaaaaa
     3961 tggtggggcg aattttttca gttcttcaaa gcacttttga gcagtatctg catcttcaca
     4021 gatgataaga cagacatcat taccacaaag ggtagcgata gcgtcaggga agctcaaagt
     4081 atcaatgata gaaccaaagg attgagccag tccaggaagg gtttttagta ggacttggtg
     4141 ttgaactggg cgcatccaga caagggcgtc ttccatgtag agttcgagac gtttttccca
     4201 ttttgagatg gaaccattgt taagaacata ataagcgcta tcttcttcgc ggacttttga
     4261 taggttcata tttttgatgt cgcgtgagag ggttgcctgg gttacttgaa tgtcgttctc
     4321 agcaagaagg gcttgcaact cagcctgtgt atgaatcttg ttttttgtga taagagcgcg
     4381 tataagttgg tggcggtgtt ctgatttatt cataataatg taactccttt tagcaaggta
     4441 aggtaagcat ggactgagcg aggtcgacag tcaagtggta gtctgtattg tcacggatgg
     4501 tgatttcaaa gtcagtagta tagaggacta aacggagagt gtctccttct tttagcttgt
     4561 aaatagttgg ctgcagttca aattgaacgt ccatccattc atctgcagta atatcctcta
     4621 ctaacagtaa atcatttcta ttttgtaaat taaggtaacc ttttgtcacg actcgttgtg
     4681 cctctggtct aaatggcaat tcacagagat tttccaacat gtgatagcga ccgttgtcaa
     4741 tggttctagc acttaaaata gctggataag gttgtaggta tttcttttgc ccaaattcta
     4801 gcagttgggc agataagagc cccttgtttg tactggattt gatacgaaga ttgagctgag
     4861 cgcgaccgtt taggtggaga tctttagtca caggaaggtt aatagtaatc tgattggctt
     4921 tcccttgata gagctctgta ttgaaggttt ggtatgtctt accatagcgc tcaaaatcct
     4981 tatctgggta ctggttttga atagcttgct cttcttgacc aagtgagaag gtttcacagt
     5041 tttcttgccc accgaagtta tcaagtgata accaagtctg tggagctgta ttgtcctgcc
     5101 agataacagt aggaagttga aagtctgttt cctgtcctag taatttcttg gtcaataagg
     5161 catttatgga ctcacggaag tcaattgatt gccaattgtt catgtaaaca tgggcaccat
     5221 tatggaaaaa gagatgcttg tgtatatgag taggaagagc atggaacatc tggtaaacat
     5281 gaagtggttt gacattccaa tcctgagaac catgagtaaa gacaacctct gcctttactt
     5341 tatgggcatt gagcagataa ttgcggtcat gccaaaactg attgtagtcc ccagtttttc
     5401 ggtctagctg agctttcact ttttctaagt cagcttggtg agcttcatta ccacggatat
     5461 agtcgccagc taagagatta cgagaatagg ttaactcagc aagggagtca aagtcctcac
     5521 ctggataacc acctgggcta gtcaccagac cgttttcacg gtagtagttg taccatgatg
     5581 aaattcctgc ctcggcaatg ataacttcta aaccatcgac tcctgtagtc gcaagaccat
     5641 tggacatggt acctagatag gaaagtcctg ttgtagcaac ttttccgttt gaccaatcag
     5701 ccttgacttg acgctggcgc gtgtgatcag taaaggcacg gcaacggccg ttaagccaat
     5761 cgatgacatt tttataagcc tcgatttgct ggtagtctcc attagtcatg aaacctgtcg
     5821 agtctttggt accaacacct gagacataga gattggcaaa gcctctcgga aggaagtagt
     5881 cgtttagtgt atagctagag ttgatgtgag ttagcttttc ctcagcctct gctataagct
     5941 cagctttacc ttggggttgg acgagattta gttgaggttt ctctagctca atcttgtgag
     6001 gaagcttaac ctcaagctcg ccctccatct tgtagagagc cttgtcacta gccttgtcat
     6061 tggttccctg atgataaggg ctggctgtca tgatggcagg gatttttcca tcaaaacgag
     6121 ggcgaataat gctaaccttt actaggtctg atagcccttt ttggtcagta tcgacacgag
     6181 actcaacgta aacgacttca cgaatgacat cctggttaga aaaagtagcc aaactcttgc
     6241 cgttaaagta gtggtagtca ttatcctccg gaataagacc atcactaaca agttggtcga
     6301 taagagtatt tccttttttg gtgcgagtat tgagtaactg atagagattt tcaatcaagt
     6361 caccatatat aatgggaaat ccagtttctt tacgaaaaac gtcactatct tcgaagtcaa
     6421 ccaaataaga aaagcctaaa agttgaaaag caacagtata aaaaatatct gctgtcagtt
     6481 catcttctga ttgaaaaaat gtcagcaggt ctgttttttt atcagctgct aggatagaaa
     6541 gtgggtagtt ggtgtcttga taagtgaaaa agaaacgacg taaaaaggtt tcaagtgagt
     6601 ctttgtgatt ggctgtattt tgtaaatcaa agccacattt ttttagttca gataagacat
     6661 tttcttttgg aaaattgata taactatatt gattaaaacg catagaacct ccatatagaa
     6721 tgacagttaa ggttattata tcaaaaaaaa agcagaaagg gaattgttaa cttcaaaagg
     6781 aaataatcca ataaaaatga ataaagtact aaattcaata tagagaacag agtaacaata
     6841 agaataaata gatagggtat aaaagttcta ggagatttat attatatgct ttctattttt
     6901 atatacaata tagtataaat ataaaaatga tgacaaaaat acaaatgaat agaaaataaa
     6961 ttagtaagct gatgaaattt ttctcaagag aagccattta taggtgaaaa tggtataata
     7021 tagtgagaag gatagaggag aagtgtaaat tgatcgcaca actagataca aaaacagtct
     7081 atagttttat ggaaagcgtc atttcgatcg aaaagtatgt gagagcagct aaagaatacg
     7141 gctacactca tttggctatg atggatattg acaatcttta tggcgctttc gactttctag
     7201 agattacaaa aaaatacggc attcatcctt tgctagggct tgaaatgaca gtgtttgtag
     7261 atgatcaggg agtgaatttg cgctttttag ctctatctag tgtgggctat cagcagttga
     7321 tgaagctttc gacagccaag atgcaggggg agaaaacttg gtcagtcctg tcccagtacc
     7381 tggaggatat cgcggtcatt gtgccttatt ttgatagagt tgagtcgtta gaactaggct
     7441 gtgattacta tataggggtt tatccagaaa cactagcaag cgaatttcat catcctatct
     7501 tacctcttta tcgggtcaac gcttttgaaa gcagggatag agaagttctt caagttttaa
     7561 cagcgattaa agaaaatcta ccgctcagag aagttccctt gcgttcgaga caagatgtct
     7621 ttatatcagc aagttcttta gagaaactat tccaagagcg ttttccgcaa gctttggaca
     7681 atttagaaaa gcttatttca ggcatttctt acgacttgga tactagtctg aaactgcctc
     7741 gttttaatcc agctagacca gcagtagagg agttgagaga gcgtgctgaa ctggggcttg
     7801 ttcagaaggg gttgactagt aaagaatatc aagatagact agaccaagaa ttgtctgtta
     7861 ttcatgatat gggctttgat gattatttct tggttgtttg ggatttgttg cgttttggac
     7921 aatcgaatgg ctattatatg ggaatgggaa ggggttctgc agtaggcagt ttggtttctt
     7981 atgccttaga catcacgggg attgacccag tagagaaaaa tctgattttt gaacgctttc
     8041 ttaatcgtga acgctatacc atgcctgata ttgatattga tatcccagat atttatcgtc
     8101 cagattttat cagatatgtt ggtaataaat atggtagtaa acatgcggca caaatcgtta
     8161 ctttttcaac ctttggagtc aagcaagctc ttcgagatgt cttgaaacgc tttggtgtgc
     8221 cagagtatga attatctgca attactaaga aaatcagttt tcgtgacaat cttaagtcgg
     8281 cctatgaggg caatctccag tttcgtcagc aaatcaatag taagttagaa taccaaaaag
     8341 cttttgagat tgcttgcaag atagagggct atccaaggca aacctctgtc catgcggctg
     8401 gtgttgtaat tagtgaccaa gatttaacca actacattcc tctaaagtat ggtgatgaaa
     8461 ttccactgac tcagtatgat gctcatggag ttgaggctag cggacttttg aagatggact
     8521 ttctgggact acgaaatttg acctttgtcc agaagatgca agagttgctt gctgaaacag
     8581 aaggtattca tctgaaaatt gaagaaatcg atttagaaga caaagaaacg ttagctttat
     8641 ttgcctctgg taatacaaaa ggtatctttc aatttgagca accaggtgcc attcgtctgc
     8701 ttaagcgtgt gcaaccagtc tgttttgaag atgtcgtcgc gactacttct ctaaatcgac
     8761 cgggtgctag tgactatatc aataattttg tggcaagaaa gcatgggcag gaagaagtga
     8821 ctgttctgga tccagtactg gaggatattt tggctccaac ctacggcata atgctctatc
     8881 aggagcaggt tatgcaggtt gcccagcgac ttgccggatt tagtcttggg aaagccgata
     8941 ttttgcgtcg ggctatgggg aaaaaggatg cctctgccat gcatgagatg agggcttcct
     9001 ttattcaagg ttcattagaa gctggtcata ctgtggaaaa agcagagcag gtctttgatg
     9061 ttatggagaa gtttgcaggt tatggtttta acaggtcaca cgcctatgcc tactcagcct
     9121 tggccttcca gttggcttat ttcaaaacgc attatccagc cattttttat caggtcatgt
     9181 taaattcttc caacagtgat tacttaatag atgcacttga agcaggtttt gaagtagcct
     9241 ctctatccat caacaccatt ccctatcacg ataaaattgc caacaaggcc atctatctag
     9301 gtttgaaatc cattaaagga gtcagtaatg atttagctct ctggattatt gaaaatagac
     9361 cttattctaa cattgaagat tttatagcta aattacctga gaattatctg aaacttcctc
     9421 tgctagaacc tttggtaaaa gttggtcttt tcgattcatt tgaaaaaaat cgtcaaaaag
     9481 tatttaataa cttagctaat ctatttgaat ttgtgaaaga gttgggaagt ttgtttggag
     9541 atgctattta tagttggcag gaatcggaag attggacgga acaagaaaaa ttttatatgg
     9601 aacaagagct tttagggata ggtgtcagca aacatccact acaagctatt gcaagtaagg
     9661 ctatttaccc gattacccca atcggaaatt tgtcagaaaa tagctatgct attatcttgg
     9721 ttgaagttca gaaaataaaa gtgattcgta ccaaaaaggg tgaaaatatg gccttcttac
     9781 aggcagatga tagtaagaaa aaattggatg tcactctctt ttcagactta tatcgtcagg
     9841 ttggacagga aataaaagag ggagccttct actatgtaaa aggaaaaata caatcacgtg
     9901 atggccgtct gcaaatgatt gcacaagaaa taagagaagc agttgctgaa cgcttttgga
     9961 tacaggtgaa aaatcatgaa tcggatcaag aaatttcacg cattttagaa caatttaaag
    10021 gcccaatccc agtcatcatc cggtatgaag aggaacagaa aaccatcgtt tctccccatc
    10081 attttgtagc taaatccaat gaattagagg agaaattgaa tgaaatcgtt atgaaaacga
    10141 tttatcgcta aaaatacgga aaatagaaga attttcaacg taaatgtggt ataatcagta
    10201 agaatgttaa aagaaaaagg agcataacca atatgaaacg tattgctgtt ttgactagtg
    10261 gtggagacgc ccctggtatg aacgctgcca tccgtgcagt tgttcgtcaa gcaatttcag
    10321 aaggaatgga agtttttggt atctatgacg gatatgctgg tatggttgcc ggtgaaattc
    10381 atcccctaga tgcagcttca gtaggggaca tcatttctcg tggtggtact ttccttcact
    10441 cagctcgtta cccagagttc gctcaacttg aagggcaact taaagggatt gagcaattga
    10501 aaaaacacgg aattgaaggt gtagttgtta tcggtggtga cggatcttac cacggcgcta
    10561 tgcgtttgac tgaacatggc ttcccagcta ttggtcttcc aggtacaatc gataacgata
    10621 tcgttggtac tgactttaca atcggttttg acacagcggt tactactgcc atggacgcta
    10681 tcgataagat tcgtgataca tcatcaagtc accgtcgtac ttttgtaatc gaagttatgg
    10741 gacgtaacgc tggtgatatc gctctttggg ctggtattgc aactggtgct gatgaaatca
    10801 tcatccctga agcaggcttc aagatggaag atatcgtagc aagcatcaaa gctggttatg
    10861 aatgtggtaa aaaacacaat atcatcgtct tagctgaagg tgtgatgtca gcggctgaat
    10921 ttggtcaaaa acttaaagaa gctggagata caagcgacct tcgtgtaaca gaacttggac
    10981 atattcaacg tggtggttct ccaactgcgc gtgaccgtgt tttggcgtca cgtatgggtg
    11041 cacatgctgt taaacttctt aaagaaggta tcggtggtgt tgcggttggt attcgtaacg
    11101 aaaaaatggt tgaaaatcca attcttggta ctgcagaaga aggggcattg tttagcctta
    11161 ctgcagaagg taagattgtg gttaacaacc cacacaaagc cgatattgag ctatctagct
    11221 tgaataagag cttgtcataa tttacaattt agttaataag accaaaaagg tcatatatat
    11281 aaaggagtca caaaaatcat gaacaaacgt gtaaaaatcg ttgcaacttt gggtcctgcg
    11341 gtagaaatcc gtggtggtaa aaaattcggt gaggacggat actggggtga aaaacttgat
    11401 gttgaagctt cagctaaaaa cattgctaaa ttgattgaag ctggtgctaa cacattccga
    11461 ttcaacttct cacacggcga ccaccaagaa caaggtgagc gtatggcaac tgttaaactt
    11521 gcggaaaaaa ttgcaggtaa aaaagttggt ttccttcttg atacaaaagg acctgaaatc
    11581 cgtacagaat tgttcgaagg tgaagctaaa gaatattcat acaaaactgg tgaaaaaatt
    11641 cgtgttgcaa ctaaacaagg aatcaaatca actcgtgaag tgattgcgtt gaacgttgct
    11701 ggtgctcttg atatctatga tgatgttgaa gttggtcgtc aagttttggt tgacgatggt
    11761 aaacttggtc ttcgtgtggt tgctaaagat gatgcaactc gtgaatttga agttgaagtt
    11821 gaaaacgatg gtatcatcgc taaacaaaaa ggtgtgaaca tccctaacac taaaattcct
    11881 ttcccagctc ttgctgaacg cgataacgac gatatccgtt tcggtcttga acaaggtatc
    11941 aacttcatcg caatttcatt cgtacgtact gcaaaagatg tgaacgaagt tcgtgcaatc
    12001 tgtgaagaaa ctggaaacgg acatgttcaa ttgttcgcta aaatcgaaaa ccaacaaggt
    12061 atcgataact tagacgaaat catcgaagca gctgatggta ttatgattgc tcgtggtgat
    12121 atgggtatcg aagtaccgtt cgaaatggtt ccagtttatc aaaaaatgat tatcaagaaa
    12181 gtcaatgctg caggtaaagt tgttatcact gcaacaaaca tgcttgaaac aatgactgaa
    12241 aaaccacgtg caactcgttc agaagtatca gacgtattca acgctgttat cgacggaact
    12301 gacgctacaa tgctgtcagg cgagtctgca aacggtaaat acccactcga gtcagtaact
    12361 acaatggcta caatcgacaa gaacgctcaa gctcttctta atgaatacgg acgtcttgat
    12421 tcagattcat ttgagcgtaa ctctaagaca gaagtaatgg cttctgctgt taaagatgct
    12481 actagctcaa tggatatcaa attggttgta actcttacta agacaggtca tactgcacgt
    12541 ttgatttcta aataccgtcc aaatgctgac atcttagcat tgacatttga cgaattgaca
    12601 gaacgtggct tgatgttgaa ctggggtgtt atcccaatgt tgacagatgc tccatcttca
    12661 actgacgata tgttcgaaat cgctgaacgt aaagcggtag aagcaggtct cgttgagtca
    12721 ggcgatgata tcgttatcgt tgctggtgtg ccagtaggag aagctgttcg cacaaacaca
    12781 atgcgtatcc gcacagtacg ttaagaaaaa tataaaaacc tatcatatcc agctttctag
    12841 cctgtatgat gggctctttt tttgtccagg gggcaaaaag ggggcaaaac tttcataatg
    12901 gattgatact cttcgaaaat caaattcaaa ccacgtcagc gtcgccttac cgtactcaag
    12961 tacagcttgc ggctagcttc ctagtttgct ctttgatttt cattgagtat gaaataagat
    13021 atgcacaaat tgattagaaa gtcaaatgaa tttctacaaa tgttttagca atcgcaatct
    13081 acttgtctag attcgatctg atatattttc gatttaatga tatggtattt aaaacctcca
    13141 aagtagcttg ctccattctt ttacttacgt gagtgtagat gttatttact gttttagcgt
    13201 ttttgtgttc cactctaacc attatagcat tcttctcagc tagtgtacta aggagtgtgt
    13261 gcctgaaaat atgggaacta aggggctggt ttatcggttt ctctagttta gtatttgcct
    13321 tttgcaaagt gatcttaaat gcctttctct aaatttacat atcactattg tttaacaaaa
    13381 tctaatctac tttaggtcac ttattctttt tttgaaatgt agaatgaact ttttcaaagt
    13441 ttttcgaatc ttttaaaatc tgtttgtttt atatcgccat tctcccccct tttttaattc
    13501 tccctatata gcctgacaac tttcccgatg gtacgaatat ggttgctttc gtctaggtgg
    13561 atgtcggggt attcgggatt gagttttttt gaggtagcct tggcggagtt tcttgacata
    13621 gttagcgccg tctacttgga agatgccgat ggtattatag tcaatctgtg gggtattctt
    13681 gataaatagg tagtcgctgt ttcttatctt tggctccatg gacttgctga cgacataagc
    13741 gattgggtcg tagtcgtctg ggataatgga aactccataa ctaaatcgtc gtcctgcatc
    13801 gagcggctac ctgcagagat aaactaccta acacgagagt aagtagtctg tctgtagtcg
    13861 tccagtctga tgatttttac gatacttcgt ttttctgatc atacagttgc ctctcggcat
    13921 aggtcagaac tttaccttgt ctgggtggtg cccgttggtc gtagatagat tggatatcgc
    13981 taggagaatc cttttgaact ggaggaaaga gggcatcaat caagctactg aatactttaa
    14041 ctaagtcaaa tatagtattt ttcttagtag acctaaccct tttttcataa tttctaatgg
    14101 tgtttttact tatacctatc ttagtaccca attcttattg agtccaacca ttactagtct
    14161 atattgtttt atagttgatt tagtttggaa tagtacgctg tagctgctaa aacatttcta
    14221 ggaattaatt tgcctttcct aatagagttg ttcatatctt atttcaatct actatatttt
    14281 tcacctctaa caatcgcaat ctcttcttta tccatgaata aaaacgattt ctatttttgt
    14341 aagtaaagca tcacacgaaa tccacgaaaa tgaaaacctt tgttgtgttt tcgtaaaaaa
    14401 tttgttgaca gagcacgaaa cgcctactat aattaaatca agtttaagga aataacaaaa
    14461 aaacaaatcg gagggaaaga cgatgaacac attaaatgag aaagtaatca atatctgtaa
    14521 agcagtagtt aaagaaactt taatccaaga catttaggag aaagaaatga agaaatcaag
    14581 aaaattagcc acattaggaa tctgttcagc tttgttttta ggcttggctg cttgtcaaca
    14641 acaacatgtt acttctgagg ggacgaatca aaggcaaagc agttcagcga aagttccatg
    14701 gaaagcttca tacaccaacc taaacaacca ggtaagtaca gaagaggtca aatctctctt
    14761 atcagctcac ttggatccaa atagtgttga tgcatttttt aatctcgtta atgactataa
    14821 taccattgtc ggctcaactg gcttatcagg agatttcact tcctttactc acaccgaata
    14881 cgatgttgag aaaatcagtc atctctggaa tcaaaagaag ggcgatttcg ttgggaccaa
    14941 ctgccgtatc aatagttatt gtcttttgaa aaattcagtc accattccaa agcttgaaaa
    15001 gaatgaccaa ttgcttttcc tagataacga tgcgattgat aaaggaaagg tctttgattc
    15061 acaagataag gaagagtttg atattctatt ttcgagagtt ccaactgagg caactacaga
    15121 tgtcaaggtt cacgctgaaa agatggaaac attcttctca caatttcaat tcaatgaaaa
    15181 agctcgaatg ctgtctgtag tcttgcacga caatctggat ggcgagtatc tgtttgtagg
    15241 ccacgttggg gtcttagtac ctgctgatga cggtttctta tttgtagaga aattgacttt
    15301 cgaagagccc taccaagcga ttaaatttgc tagtaaggaa gattgctaca agtatttggg
    15361 caccaagtat gcggattata caggcgaggg actggctaag ccttttatca tggataatga
    15421 taagtgggtt aaactttaat agtgttatga tatgacagta atcttaggat ttttatttct
    15481 atttttgatg acttggtggg ttatttcaat cgttaatagc cagaaaccac gcaaaaatga
    15541 aacatttatt ggctatatcc aaagatacga tattgatggg aatggctatc aatcattacc
    15601 gtgtcctcag aactgagagg agttcttgaa atcgtaaaac tactttgaac aagagttact
    15661 tcaacccatt ggctccgacg gattaagttg ctttcgtgaa caccaaaatc agccgcaatt
    15721 tcttcataag tgcggtattc tcgcacatat tgaagagtgg ccataagaag gtcttctagg
    15781 cttaatttag gttttcgtcc cccttttgtg tgtttaagtt gataagcagt ttttaataca
    15841 gctaacatct cttcaaaagt ggtgcgctga acaccaacaa gacatttaaa tcgtgcatca
    15901 gttagttgct tacttgcttc ataattcata gaactactat accatatttt gtttcgcagg
    15961 aagtctaatg ctaaaatctt atcagtgaga agatttctta aagtttattc tttgtagttt
    16021 catctgtatt agtatataat ttaacttaga gaaaatagga ggactaatcg aatggaatta
    16081 aaagatttta cagaaaaaga acaggaaatg attaagaaaa ggcttacaat gtctaatatt
    16141 agtgacaaag aaactactga gaagattctg gcgctagtac cacaagactt gattaagcga
    16201 atcccgtttt ttgtcagaaa acatgctaca acacgtacga ttaaacgcat ttcaattgaa
    16261 caccctgaac tctacgctgt agctcaaaca agtggtgaga ttccagaaaa agaacgcgaa
    16321 gaattgcgtc agattatcac aactatcttt gaacaaaaga tgaataagca tagtattaag
    16381 tagagaatga aaaaatattt tattggcggt ttgggaagca atgcctatca tagcaaggat
    16441 tttcttcaag aactagattc gcaggtctat tttctaaatc catatgaaaa gcatcttcga
    16501 gatgaaacag aattgaaatc atggtttaaa aatgagattg tagaggaaga atctatctgt
    16561 ctgataggtc attctattgg aggcgattta gctcgttatt tcgcatcgga atttgaagaa
    16621 gtaaagaaac tgattctttt ggatggtggc tatctagatt tagataagat tttacctttg
    16681 gatacagagt tagaggaaac taaaaattat atcaaatctc aaattatttc ggacttagat
    16741 gttcttactt ctaaagaaaa atctgaagca aagcattggt cagaaaatat ggagaaagct
    16801 gtaagacagt cctatcactg gaatgttgag tataatagat atgagttggc tataaattat
    16861 gaaaatatag aagcgatact ccgcctacgg agaaaaatac aagcttttaa gagagaagtg
    16921 ggagatacct tgtttattag tcctcgctat cctaatgaag ctacatggag agaggaagcc
    16981 ctaaaagaat tgccagacta ttttgatact atttttttag aaaactttgg ccatgagctt
    17041 tatactcaag cacctaaaga aatcgctagt ctaatgaatg agtggctcgc ttattttcta
    17101 tgagctactt gtattgctgg aaatggtata agatgaaaat ctacaagtca ctaattttca
    17161 tataaccaaa aaacgaacag gctctttttc tgggctcttt gtcaactgta gtgggttgaa
    17221 gtcagctaag ctcgagaaag gacaaatttt gtcctttctt ttttgatatt cagagcgat
//
LOCUS       DAVLYV010000036        16963 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLYV010000036 DAVLYV010000000
VERSION     DAVLYV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.036"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            122..300
                     /locus_tag="VYO70_001195"
                     /pseudo
     CDS             122..300
                     /locus_tag="VYO70_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            382..699
                     /locus_tag="VYO70_001196"
     CDS             382..699
                     /locus_tag="VYO70_001196"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEV1713430.1"
                     /translation="MLAVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEI
                     ATDFGIHESNLIRRSQWVEVTLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKTISE
                     SFW"
     gene            716..1105
                     /locus_tag="VYO70_001197"
     CDS             716..1105
                     /locus_tag="VYO70_001197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1713431.1"
                     /translation="MKAQAIVTSQGRIVSLDITVNYCHDMKLFKMSCRNIGQAGKILA
                     DSGYQGPMKIYPQAQTPRKSSKLKPLTAEDKACNHALSKERSKVENIFAKVETFKMFS
                     TTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            complement(1175..1537)
                     /locus_tag="VYO70_001198"
     CDS             complement(1175..1537)
                     /locus_tag="VYO70_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713432.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1915..3795)
                     /locus_tag="VYO70_001199"
     CDS             complement(1915..3795)
                     /locus_tag="VYO70_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1713433.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3789..4658)
                     /locus_tag="VYO70_001200"
     CDS             complement(3789..4658)
                     /locus_tag="VYO70_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1713434.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSIPTPKNLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHCFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAGKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNCNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4741..7386)
                     /locus_tag="VYO70_001201"
     CDS             complement(4741..7386)
                     /locus_tag="VYO70_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1713435.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFELFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVQLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAYRRAKALQTSFTSLQLARQEGS
                     VVAIGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7477..8757)
                     /locus_tag="VYO70_001202"
     CDS             complement(7477..8757)
                     /locus_tag="VYO70_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1713436.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDSLLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8928..11012
                     /locus_tag="VYO70_001203"
     CDS             8928..11012
                     /locus_tag="VYO70_001203"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1713437.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLNKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11052..12731)
                     /locus_tag="VYO70_001204"
     CDS             complement(11052..12731)
                     /locus_tag="VYO70_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1713438.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGSLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGENAYFTVKRRECSGPLEAKISIQPGTGVHGVAYQ
                     DEIYVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13486..14715
                     /gene="arcA"
                     /locus_tag="VYO70_001205"
     CDS             13486..14715
                     /gene="arcA"
                     /locus_tag="VYO70_001205"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1713439.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDESKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14773..15789
                     /gene="argF"
                     /locus_tag="VYO70_001206"
     CDS             14773..15789
                     /gene="argF"
                     /locus_tag="VYO70_001206"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1713440.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15954..16901
                     /gene="arcC"
                     /locus_tag="VYO70_001207"
     CDS             15954..16901
                     /gene="arcC"
                     /locus_tag="VYO70_001207"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1713441.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
BASE COUNT         4877 a         3852 c         3447 g         4787 t
ORIGIN      
        1 gaaattaaag tgggttaaaa aagaaattaa ctgagtaaaa actctcgaaa ttcaattagc
       61 tatattatca ctcaacttga acaaatacca gaaccacaaa aaacagatgt aaaggaaaaa
      121 tatggactta aaatttgaag gggttgattt agaatacaaa aaagctaaaa acaatcttcc
      181 agagtctttc tgggaaacct actcagcatt tgcaaataca aatggcggga aaataattct
      241 cgggatagat gaaaaaaaaa cattgacccc taccaaggag tcaatagact tcctgcgaaa
      301 caaaattatg aagcaagtaa acaactaacc gatgcacgat ttaagcgtct tgttggtgtt
      361 cagcgcacca cttttgaaga gatgttagct gtgttaaaaa cagcttatca acttaaacac
      421 gcaaaaggtg gacgaaaacc taaattaagc ctagaagacc ttcttatggc cactcttcaa
      481 tatgtgcgag aataccgcac ttatgaagaa attgcgactg attttggtat tcacgaaagc
      541 aacttaatcc gtcggagcca atgggttgaa gtaactcttg ttcaaagtgg tgttacgatt
      601 tcaagaactc ctctcagttc tgaggacacg gtaatgattg atgcgacgga agtaaaaatc
      661 aatcgcccta aaaaaacaat tagcgaatca ttctggtaaa aagaaatttc acgctatgaa
      721 ggctcaagcg attgtcacaa gtcaagggag aattgtttct ttggatatca ctgtgaacta
      781 ttgtcatgat atgaagttgt tcaaaatgag ttgcagaaat atcggacaag ctggtaaaat
      841 cttggctgac agtggttatc aagggcccat gaagatatat cctcaagcac aaactccacg
      901 taaatccagc aaactcaagc cgctaacagc tgaagataaa gcctgtaacc atgcgctatc
      961 caaggagaga agcaaggttg agaacatctt tgccaaagta gaaacattta aaatgttttc
     1021 aacaacctat cgaaatcatc gtaaacgctt cggattacga atgaatttga ttgctggtat
     1081 tatcaatcat gaactaggat tctagttttg caggaagtct attgaaatac taagcctaca
     1141 cagactgatt ccaattgttt tttagatttc aattttacca ttcttcatag tcaaaaccat
     1201 cataatctgg atattgttct ggcgacgttc caaccgaacg tacgagataa gcttcttcgt
     1261 cctctgtctc gatttctctc agcacttggc attcaaaacg ccattcatca ccgaagtcga
     1321 aaataaactt aaatttttgt ttgacactta ggctatccag atagacattt tctgtgtaac
     1381 gttcttcaac atcgtcacta acaaagctaa gaaagtaaga atctgcatga ctccactcaa
     1441 cattatccat gaaaaatgcg tgagcatgat catttgcaaa atcaaaactc cataaaatca
     1501 catctgctag ttccgctaac gtttttgttt ttggaatcta tatatgtcgg tagcaacctt
     1561 ttttaaattt gacggaaatg atccaagctt ctacagatgc tggcttttca gcttctattt
     1621 cttgttttac taattttaag tgtagcataa tacctccaat tgctcatcga tagaattgct
     1681 cttaggaaac attcactttc tgctatttta tcataagttt gtctaactgt agcatttgaa
     1741 accagataat tcttctgttt cacgcgaaac accctgcgcc aagcaaggtg ttcttgtttt
     1801 tatactcaat gaaaatcaaa gagcaaacta ggaagctagc tgcaggctgc tcaaaacact
     1861 gttttgaggt tgtagatgga agctgacgtg gtttgaagag attttcgaag agtattaagt
     1921 cgtataaatc gtcgacgctg tcgcaatggt atgccactgg ttggctgtag ttgctgcgaa
     1981 atccttgtct gcgtagaagt cagtaaatgg taggatttca acttccagct cgtcgatgcg
     2041 gtcaagctga ccagccagat aaacctcgat acggctttct gctcgtttga tgcgttgcaa
     2101 gagtccgccc atacggatgt caactgtatc caaaccaaag accttgtttt ctttcagcca
     2161 ttggtgacta aagagggcat ggaagtcttc aatttggctt ctaagttctg gtaattcttg
     2221 tctggcgatt tgttgtaaac tttctttatc atccgcttgg taggcctgac gaatgcgtcg
     2281 tcccacatct actttgctac ttaaaatagc attcaactgg gcctgagttt caaagagata
     2341 ggcatagttt ccagcttttt ctttaatgtt agcaagcgtc tcagcagcct gagcgaagtg
     2401 cggtttgtcc tgttcaggtg tcatgtgttg atcaagaatc ggacaaagaa tatcctgata
     2461 aaaaacatag cggttgggat tgataccgct gagattgcct ggtaggtctg gtaagaggtt
     2521 ggcaaggtca atctgcataa aatcctcaac cgttagacca gtattggtct tgaagtgcgc
     2581 agacaaacca tctaggtcat tgcgatagct gagttctgcc cagatttgca agcttggtag
     2641 gatagagaac tgggcagttt caccaccatt gtctccccaa cccgttacga tgacttcttt
     2701 aatctgattg gcacggcagg ctttattagc ctcgatagcc actagacggc taaaatggtt
     2761 gtgaggtgta aagccaatcc acttccaagc tccccctgca aatgcaaggt catggctaat
     2821 cttgtgatga ttgcggaaat tacggttgta tttttcctcg ctatcctgat aataatccca
     2881 gtaaaccaga gtcacacggt ctttgagacg gtctaggtag acacgagttt cctctggaat
     2941 ttccacatca cggtcgtact ggccatccgc tgacatgagt ttgaagaaca tatcactcca
     3001 catctggcag tggaaaccat atttgtcagc aatatccagc acgcgctcca agtgttggca
     3061 catgaggaga ctacgatcca caacaccgtt caaaatcagg tagcgtccca aaccaaccaa
     3121 gtgggcttcg tccatcccga tattgacctt gcgagtcttc agtttagaca acgtggcaaa
     3181 catgccatca atcaggtcat aaaccttttc ttcgccaatg agaagaatgt cctctacatc
     3241 acggagctcc tgcacttcct tgacacccca tttgacaaag gccgacaagt gggccaaggt
     3301 ctggatgcat ggtacaaagg tcatgtcaaa ctgttgagca taggcttcta tttcctgcaa
     3361 ctcctctgct gaataagctc cacggaaata gccaaagtaa ggctgtcctt caatctggta
     3421 agtgtcttcc atgtaaagct caaaggttga gtagcccatg agagccaaga cctcaatcat
     3481 ctgcttggca gaagccacat tcagcaccgc atttcgagaa cagtcaacca tgtaagccaa
     3541 atcttcgtaa gctgcttgtt cctcaatctc tactttatca gcttctgcta gaactgttac
     3601 caacaaggat aaggcacgat aaagttggtg aggtttgcgg taggtcaatt gatagtgacc
     3661 ttcctcaccc tggatagaga tagaggcttg gtcagactga gtgacagcca cttccacatc
     3721 tggtagagaa atgtgacctt ttaaaacctc aatagcttgc gtttgtttgg gactaagtcc
     3781 tgtaaatctt accattgctt ttcctcctgt agccagttga caagagcacc gtagagattg
     3841 gcatctgcgt gataggtgca ggcctggata actggtgcga ccgtgtattc ttcgtaggca
     3901 tctacaaagt cttcaacagc cttcttaaca ccttggataa aatctggatt ttgactgata
     3961 gagccaccca gactgatgac atctggatcg atcagatact ggatattgag caagccttgc
     4021 gccagattgc agttcatgcg ctcaatggct tcttgacaaa gagcattacc agctgcggcc
     4081 tcttggtaaa tcttgcgacc gtcccaatca gtatgaccag atttttcaat tacgtatcgt
     4141 accatattcc cagttgatgc tagttgcgac cagttattaa gttttccagc aggggcaagg
     4201 gttgtcatgt agccaaattc tccacccaga ccgtggcgac ctcgatgaag tctaccattg
     4261 ataatcatgg ctccgccaat ccctgtccca atcacgacac aggctgcatt ttcaagctct
     4321 ggatgagcta gtagttcact gagtccaacg cagttggcat cattttctaa atggacaggt
     4381 agctgataag agctaagcgc ctcataccaa gaaaagcaat ggatgtaggg caccgcactg
     4441 aagccatcaa tcacacctgt ctcttgattg accgcacctg gaacgctcat agcaatccca
     4501 ctataatcct gttctgacaa acgttgatct agccaagcta gtaagtcctc caagtttttt
     4561 ggtgttggga tacttgtctt atctagtatt ttcccatcag gagtcagact agcaaactta
     4621 atcccagtcc ctccgatatc aatcgttgca atggtcattt ttttaccata aaaaggggca
     4681 aatcagcagt tagttcttac cttttcgccc ctcctttctt ttgatgttta ctttttgaaa
     4741 ttaaatttct tcttttttga tgaattctgt acgaatctct tgtggagcca atagtcctga
     4801 atgaactgga tatggtcgct caagtaagtc aagaatggtt tgttgatgtt ctgatatgcg
     4861 cacattttct tgactcatat tatagtaacg gaggacatag ccttcttcat tttccgccac
     4921 tttaaaggct gttggacaaa cttgcggtat gctgagaaca gaatggctca agaggctacc
     4981 aatcgcaacc acgcttcctt cctgtctagc aagctgaagg ctggtaaacg atgtctgcaa
     5041 ggctttggca cgacgatagg ctgagaagcg ttcttgggct tggtggcatt caagtgcaaa
     5101 ctcgacttca aactcccgca agcattgtgc ttctggcgtt gggaagtagc cccagtcacc
     5161 tagctcacct gatgcacgca aaatggtcac ggcaatggtg ttatccccaa ggatttcgta
     5221 ttcattcaat cccttattgg atacagtcac ccctttttca tcgtcataca gactgacaaa
     5281 agcttgttgg tgttgaggat tttcaggatt ttcccatgaa gcagctggtt tgtttggtcg
     5341 tgtcaccacc tcatagatac tttcagaatc attacttgga cgcgtgttat gagtcttgac
     5401 caagagacgg atacggtgat ccttggcagt gttggtaaag cgagtcttga agcggatttg
     5461 tggattgtca acgaagacag tcaactcagt ttccagagga atgtttgtca attcttctga
     5521 ccgtccagcc tcacgcttca taaactcgat gataccttgt tgctcttctt ctagcttttc
     5581 atccgcgctg acaggcacgg tcaattcatg tttgagcaag attttagcat agcaagctgt
     5641 gttttccaag acctcgtggc ccttaagctc tgcaaagatt ggctctgttc cttttggttg
     5701 gaaatagata tactcgtttc cgatgtcccc acggtcttca aagcggataa agtcttcata
     5761 ggcttcgtga gttgtcttgt catagactgt gatgttgtca tccacactca ccgttacgaa
     5821 tggtgtatca atcactccgt tttggtaaat accctcacgg tgttcttgtt ttccttccag
     5881 caattggaag gttgtccaag aaagcggcgc tagctgaact ggaatggtca cgcgcacttg
     5941 tcgagcaata cgagcttggc ggaacttgtc ttttggtaaa tcgtattcaa aattagctcc
     6001 gaggtcttcg attgtagcct ctacaggacg accatccaag tcctccacac ggtaacttgg
     6061 caagataaga gcggccatct ttttgtagcc ttctgttggg tgcaattcct tgaaatcaca
     6121 agtcgccaca tcaatcactg tgctgacagt atcgacctta tcatgcaagc ctgtgttaat
     6181 gacagtaaag agatagtcac tttgagcctt atccgtagca attttaccct tccactcgtt
     6241 gagcaaatta cttttaacaa agtttcctac ttggttgacc ttggcaaaac gcgtttccat
     6301 ctcgcggtga acttcgtcca cgctacagcc acagatacta tcatgtggcg cattctgcaa
     6361 aagtgttttc caagcatagg tcaactggtc cttgtggttg tgtccaccag tgataatagt
     6421 caagggttct accacttgct ctaggaggtt gctattttct tggaaggctt gttttaggta
     6481 aatgcgggat gaagaagtgt tggcaagtgt gtaccagcca tctgtttcct gactggtcaa
     6541 ctcgcctgta acagttgata agtgttcagg aagcgcacct tctacagctt gaacatattc
     6601 atcaaaagaa ctatgaacaa agattacatc tgggaagagt tcatttgcca cacgaatggc
     6661 ttcgctcaga tttttctgta caggctggtg gtcacagccg ttcatcatca accattggtt
     6721 ggtcgaagcg taggcacgca catctgacaa tttttgtttc cagaaggtca aggcctcatc
     6781 tttgtcaact gggatttcat tcccattact gtaccagtta gcaaagagaa tacctaaaac
     6841 acgactacca tccacaccct gccagtacat ttctgaaaac tgggatgtaa actgctcatc
     6901 ttcaaggact tggttgtcaa atccaatcgg cttcacacca cgaccaaagg ccgccacgtg
     6961 aatgcctgat ttttgaagaa tttgaggcgc ttgtcccata tttccaaagg tatctggaaa
     7021 gtagccaatc tgggttgatt taccccattt ggcagcttct tgttgaccaa tcaaggtatt
     7081 gcggacattg gcttcactgg agatcaagta gtcatcctgc aagatgtaaa agggaccaat
     7141 tttaagtttg ccctcgtcaa tgtagcgttg gaccttgtcg cgattttcag ggcgaatttg
     7201 taagtagtcg tcaaggacaa tagtttgtcc atccaagtgg aaactcttga actcagggtc
     7261 attttcaaag agttcaaaga gattgtcaaa caattccacc aactgcatac gatggctttc
     7321 aaaaggcaag taccactcac ggtcccagtg gctatgtgag ataatatgta caacaacatt
     7381 ttccatgaag taaaacctca ttctaaattt aaattttaac gttttaaatg taaacttgtg
     7441 attctgataa gaatcggttg agctaaagcg agccccttag cgaatatcta aataatccaa
     7501 gaccaactca cagaacatca tgttagccca ggagaaccat tcacgagagt agagggtcgg
     7561 atcatctacg tggaagcttt cgtgcatgac acctgtacca ccatcgcagg caaccagctg
     7621 atccagcaag aattttttct ctgccttatc tcttgttgtc aatccttgga tagaaagggc
     7681 gattggccag atatagcgat agaaggtatg agaacttccg agaccgctag cgtattctcc
     7741 ttggtagaaa tatggatttt cagagctcaa aatggtacga cgagtagctt gatacacttc
     7801 atcatcgacc gaacagtagc ccagataggg cgcagctagt agacttggta catttggatc
     7861 atccatgatg ctggcatttc ctaggccatc cacttcaaaa gcgtaaatct tttcgccctt
     7921 gctgttggtg gtgtaagcgt agttttcgat tccttcttgg atttcatcct gaagacgctt
     7981 ggcatcagca ataacactct ggctatcagc taggtttaat gctgcgaaga tttcttgcac
     8041 ataacccaag actactacag caaacatatt tgacggaatc aagtaactat actgacagca
     8101 gtcatcgctc ggacgaaagg ctgaccaagt cattcctgtc actgcaaaat caggtccaaa
     8161 gccatcattt accaaggtgt cttccttgcg gtccgtatct cggacaaaac gataaggaga
     8221 gttcttatga tcttgttcta ccgtccacag atggagaatt tccttagtcg ctgcgacaaa
     8281 aatctcatca aactgactag tctcgccagt ctctttccag aggagataag ccaactgcaa
     8341 aggatagcaa agcgaatcca cctcatactt gcgctcccag atccagccgt taaggtctgt
     8401 gtggtcagtc tcgtggtgcc ctttccagtt ctcctcaatg ttgaaggagt tagcataggg
     8461 atccttgagt accaaggtca tctgacgttt gaccaaacct gcaatggtct gacgcaggag
     8521 ggaatctctt ttagccacat gcaggtaggg tctgagttgg gctgtcgaat cccgaagcca
     8581 catagcagga atatccccag tcaagacaaa agttgaacca tcttctaaga tttcaaccgt
     8641 attgtccaag gtatcggtgt agcaacgctc gaaaacatcc acccactctg gatggtcctt
     8701 ggcccgctct gctacttcat ctagccattc tctaacaatt tctttcgaat aaatcatcgt
     8761 gcaattccct ctttcaaaca agtttcatgt tcagtatata gcttttgaga cagaaaatac
     8821 atacacaaat gatagtcatt tttctacaaa atagttattt ttgaaactcc ttttctaaag
     8881 gccctctttt tctgatataa tgtagaaaaa cagctaaagg aaagactatg aaaccactac
     8941 ttgaaaccat cgatacccgc tttggtactg ctagcaagca tgccttctct cggggaaata
     9001 ccctgccata cacaggcgtg ccttttggta tgaattactt tgtgccccag accagtgacc
     9061 aggatggatc ttggtttttt gatccgcatc tgcctatctt tcaggggatt cgattaactc
     9121 accagcccag tccttggatt ggggactact cttggctcct tctgacacct gtcaccagtc
     9181 aactgggggg agacagcctc tttcaccgcc agtcttctta tgatatagat aaagcttgct
     9241 tccagcctca ctatttgaag ctcttttctc tgcgttatca gattgaaacc cagctcacac
     9301 cgacttgcta tggtgcttct attcgtttga atcaaaagca aggcaaagcc ctctccctct
     9361 atcttcacgc agcagatgaa ctgacagtag agcaagtaga taagcggact cttgccctgc
     9421 gacaagaagg taaaactgaa actaacaaaa attcgctaac gatgttcact gccctgcaaa
     9481 tgaacacgga tattcttgct atcagccaag aagctggaga ctggcgaatt gacttagcaa
     9541 gtagtcaaac cgagatgcaa ctagccactt ctttcatctc tccttctcaa gctctaatca
     9601 atctacctca agaagatttt gatagctgta aaacaagtgc ccaagcggat tgggaaaatc
     9661 tcctccatcg ttttgacatt atagagacag gagaggctga ccgaaccttc tttgaccact
     9721 gtctctacag actcttcctc ttcccacaga ctttttatga ggttgatgag tcagggaaag
     9781 ccatccacat ggatctggct actggtactg tcaagcctgg tgtcctcttt agcaacaatg
     9841 gtttctggga taccttccgc accaccttcc ccctctttgc ccttatcata ccggagcact
     9901 atcaacgctt tttagaaggt ttcctcaata gctaccgcga tactggtttc cttccaaaat
     9961 ggctggctcc agatgaacgg ggtatgatgc caggtacact gctagatggc attatcgcag
    10021 atagcgcctg taaggacatg gcccccgacc tagaaggaga actcttccaa gccatgctcg
    10081 aaacagccag caaagccgac cctctcggca tcaatggccg ccacggacta gcccaatacc
    10141 aagaactagg ttacctctct accgaccacc atgaaagtgt tagccatact ctagactatg
    10201 cctatagtga cttttgtatc gccagctgtg ccaaaaaact agagaacata gaaatcgctg
    10261 aaacctacaa ggctgcttca caaaattacc gccagctatt tgacgctgag acaggttaca
    10321 tgcgagcacg agacaatcaa ggaaactttc accctgactt ctctccttat agttgggggc
    10381 gagactacgc tgaatgctct gccattcaag ctactttagg cgttctccac gacatccctg
    10441 gcttaatcca actgatgggt ggaaaagaaa cctttagcaa ctatcttttg aaagcctgtc
    10501 aagatgctcc cctctttgaa acaacaggct atggttatga aattcacgaa atgagcgaga
    10561 tggctactgc tccttttgga caaatagcca tttccaacca gcctagtttc cacatccctt
    10621 atctcttccg ctacagcgat taccctgact acactgccct tcttatcaag acgctccgtc
    10681 agaaagcttt tcacccaagt tgggaagcct atcctggaga tgaagacaat ggtagtctct
    10741 ctgcttggta catctggtca gctctcggat tttatccaac ctgtccagga aaaccaagct
    10801 atgacctcgg aatccctctc tttgaccatc tacgagtcta cctagctaaa gaagataaat
    10861 ggctggatat ccatactaaa caaaaccaca accattttaa ctttgtcaaa gaatgccgac
    10921 tgaacaaaac actcgtatca actattcaac accaagacct cttaaaagct gaacaactaa
    10981 ctttcaccct cagctggcta ccaagtcact aataaccaaa agaacctttg catcgcgcaa
    11041 aggttcttct tttatgaaac ttggacttga agttgatcca caagctgacc atccaccgtt
    11101 aggttcagat agaaatccaa ggttttatct cctgcgaaat acagggttgg tagatgcaaa
    11161 tcttttttgc tttcccctgc ttggaattga aggacataaa tctcgtcctg ataggcgaca
    11221 ccatggacac ctgtccccgg ttgaatcgaa atcttagctt ctaaaggacc actacattct
    11281 ctgcgcttaa ctgtaaaata ggcattttct ccctttgcca cagctaggct tttttctgca
    11341 aatgctagtt cctgaacaac ttcttttttt gataatccag gagttttata aagggaaatc
    11401 ttggtcaata caggaataga ttgtgcctcg gtaatcatca cgcgcacctt ttgtgcctct
    11461 actagcgaac ctcgcaagag gcgcttatga ccaactgtga agcccctgcc aaattcctgc
    11521 cagacaccat ccacctctac ttgcacatgg aaagcagaaa tacgttggcc taacttcaaa
    11581 tcttctctca actcaattac atcaaaagtt ttaggagaac ctaagtcgag ttctaactgg
    11641 atgggcaagt ctgcatcgtt tgcccaagag ctggtctcaa ggccgtctgt caaatggcga
    11701 caagcaaagt ctgcggaaag agctggacca gatacctcag ctcccagagc caaatcttct
    11761 ttatagagct cattgcgata ggtcgcaaat tcataaagtc gttcaatatc ctttgcatca
    11821 aagagcccag cttgattcgg cggaatatta agcaagagtg gagttcctcg ccctactgag
    11881 tgaaagtaga tttcgaccaa ctcctcgaga gacttaggat cctgatcctc atggtagaac
    11941 cagcctggac ggatggaaac atctgcctct ccgattgaaa aaatcgtgcc cgagggatcc
    12001 ccgtgctgaa gatagttcag ctctgcttct gttcctagtt tatcaggatt caccttttgc
    12061 cacagtggat cacctgcata ccctcgttca ttgccaatcc agcggatact ggtgccttct
    12121 gttgaaaaaa tcaagcaatc gccctgcagg tcacgaatgg tttcaaacca tttttcaaat
    12181 tcataattaa ccttttgcgc gccctctcct ctggcaccat ccatccaaac ctcagcgaac
    12241 ttaccagcat tcccatagtt aggatttgat aagatttcct tcaactgagc cagataatag
    12301 gcattgtagt ccgcttctcg gtccacatga tagaggggac tatgggcatc ccacggtgac
    12361 aggtagaccc ccatatccat atcaaactct gtggcagctt gggatacttc aaggagcaag
    12421 tcgccctttc ctctcctcca aggactgacc ttaaccgaat aatctgtgtg agctgtcgga
    12481 taaaggacaa agccatcgtg gtgcttgacc accaaaatca actttttgaa gcccgtttcc
    12541 ttgagcacac gaacccactc acgcgcatcc aactgactcg ggttaaagcg ctcaggatcc
    12601 tcctgtccag tcccccattc ttggtcataa aaagtattag gaccgaagtg gataaaagct
    12661 gctagttcat ctcccagata agctagctga gtctgacttg gtaacggtcc atgcggtttg
    12721 attttcttca tcgttatata tctccttctt tagcaacttc tcttgttcac aaggttatcc
    12781 acaggttgtg gataataaac catgatctaa cagccagctc tttccaactc caactggtag
    12841 gctcaggatt ttgctcctca atattccctc ttcaaacagc tctgtcatat ggttctgcta
    12901 acacctctca gaatgctact ctccataatt attctcaggc ttctttcagg tcattgtgtg
    12961 aagaccattc actctccaga cttttccaca gattgtgaat aagtgattat tctaaactat
    13021 cacctcgtcg actataacaa aaaataaaaa gagggtcaat aagataatta tctcttccta
    13081 aatactcaga aaagattcta taatttgtac aattgaacaa gaaatgcttc tccaaaatct
    13141 atcttcaaaa gaatgttgca tctttttatc ctttcacaca aacatttctc tttatacaga
    13201 tccaagtgat aaccagatga taattggaca ctttttggtc atttgtatat tttttgaaaa
    13261 attttttaaa aaaattttat atccttttta tagaagttta tgcatttgta tctatgtatt
    13321 ctatcctatg ttatgtataa taacataaaa ttgtgctaaa catcataatt tatattattt
    13381 tccgagaaat tatagccatt gacattacct aggaattgcg ttataacata catgtaagcg
    13441 gtacccaaaa tggtgccaag tcaaaatttt taaggaggaa aatacatgtc ttcacatcca
    13501 attcaggtct tctcagaaat tgggaaactg aaaaaagtta tgttgcaccg tccaggcaag
    13561 gagttagaaa acttgttgcc ggactatctt gaaaggcttc tttttgatga tattcctttc
    13621 ttggaagatg ctcaaaaaga acatgatgca tttgcccaag ctcttcgcga tgaaggaatt
    13681 gaggttctct acctagaaca actcgctgct gaatcattga cctctccaga aatccgcgat
    13741 caatttatcg aggaatactt agacgaagcc aacatccgtg atcgtcaaac caaggttgct
    13801 attcgtgaat tgcttcacgg catcaaggac aaccaagaat tggttgaaaa aacaatggct
    13861 gggattcaaa aagttgaatt gccagaaatt cctgacgaat ctaaagatct aactgactta
    13921 gttgaatcag attatccatt tgcaattgac ccgatgccaa acctctattt cactcgcgac
    13981 ccatttgcaa caattggaaa cgccgtatcg cttaaccaca tgtttgcaga cactcgtaac
    14041 cgtgaaacac tctacggtaa gtatatcttc aaataccacc caatctatgg cggaaaagtg
    14101 gatttggtct acaaccgtga agaagatacg cgtatcgaag gtggagacga gctagttctt
    14161 tctaaagacg tccttgcagt aggtatctct caacgtacag acgcagcttc tatcgaaaaa
    14221 cttttggtca acatcttcaa gaaaaatgtt ggcttcaaga aagttttggc ctttgaattt
    14281 gctaacaacc gtaaattcat gcacttggat actgtcttca ctatggtaga ctatgacaag
    14341 ttcactattc acccagaaat cgaaggcgac cttcacgttt actcagttac ttacgaaaac
    14401 gaaaaactta aaatcgttga agagaaaggt gacttagctg aacttcttgc tcaaaacctt
    14461 ggtgtagaaa aagttcattt gattcgttgc ggtggtggca atatcgtagc agctgcgcgt
    14521 gaacaatgga acgacggttc taacactttg accatcgcac ctggtgtggt agttgtttat
    14581 gaccgcaata ccgtgaccaa taagattttg gaagaatacg ggcttcgctt gattaagatt
    14641 cgcggaagtg aattggttcg gggccgtggt ggacctcgtt gtatgtctat gccatttgaa
    14701 cgtgaagaag tgtaatcgct gttcggtatt cgtcaataga aaatgtaaaa aatagaaaga
    14761 ggaaataata aaatgacaaa ttcagtattc caaggacgca gcttcttagc agaaaaagac
    14821 tttacccgtg cagagttaga ataccttatt ggtctttcag ctcacttgaa agatttgaaa
    14881 aaacgcaata ttcaacacca ctaccttgct ggcaagaata tcgctctcct atttgaaaaa
    14941 acatctactc gtactcgtgc agcctttaca actgcggcta tcgaccttgg tgctcaccca
    15001 gaatacctcg gagcaaatga tattcagttg ggtaaaaaag aatctactga agatactgct
    15061 aaagtattgg gacgtatgtt tgacgggatt gaattccgcg gattcagcca acgtatggtt
    15121 gaagaattgg cagaattctc aggcgttcca gtatggaacg gtctaactga cgaatggcac
    15181 ccaactcaaa tgctcgctga ctacttgact gttcaagaaa acttcggtcg cttggaaggc
    15241 ttgacattgg tatactgtgg tgatggacgt aacaacgttg ccaacagctt gctcgtaaca
    15301 ggtgctatcc ttggtgtcaa tgttcacatc ttctcaccaa aagaactctt cccagaaaaa
    15361 gaaatcgttg aattggcaga aggatttgct aaagaaagtg gcgcacatgt tctcatcact
    15421 gaagatgctg atgaagcagt taaagatgca gacgttcttt acacagacgt ttgggtatca
    15481 atgggtgaag aagacaaatt cgcagaacgt gtagctcttc ttaaacctta ccaagtcaat
    15541 atggacttag ttaaaaaagc aggcaatgaa aacttgatct tcctacactg cttgccagca
    15601 ttccacgata ctcacactgt ttatggtaaa gacgttgctg aaaaatttgg tgtagaagaa
    15661 atggaagtaa cagacgaagt cttccgcagc aagtacgctc gccacttcga tcaagcagaa
    15721 aaccgtatgc acactatcaa agctgttatg gctgctacac ttggtaacct ttatattcct
    15781 aaagtataat tttagataat aaaccgtcta ccaacagcta tgagggctgc gactaatagc
    15841 tttagtccgg tcctctttta tataatggta atctattatt tcttataaaa tatgtgaaaa
    15901 atcattaaat tgaaatctaa acgcattcta ttgagtgtga taaaggagaa tttatggcaa
    15961 atcgtaaaat tgtagtagct ttgggaggaa atgcgattct ttcttctgac ccatcagcaa
    16021 aggctcaaca agaagcttta gttgaaacag ctaagcatct tgtaaaattg attaaaaatg
    16081 gagatgattt gattatcact cacggtaatg gacctcaagt tgggaatctc ttgctccaac
    16141 atttggcatc agactctgaa aagaaccctg ccttcccact cgactcactt gtcgctatga
    16201 cagaaggtag catcggtttc tggttgaaaa atgctttgca aaatgctctc ttggatgaag
    16261 gcatcgaaaa aaatgttgcc tctgttgtaa cgcaagttgt cgtagataaa aatgatccag
    16321 cttttgttaa cttgagtaaa ccaatcggtc ctttctattc agaagaagaa gcaaaagcag
    16381 aagccgaaaa gagcggagcg actttcaagg aagatgctgg ccgtggctgg cgtaaggtcg
    16441 ttgcctcacc aaaacctgtt gacatcaaag aaattgaaac catccgtact cttttaaata
    16501 atggtcaagt cgtcgtagct gcaggtggtg gcggtattcc cgtcgtcaaa gaaaacaatg
    16561 gacatttgac tggtgtcgaa gcggttattg ataaagactt cgcttcccaa cgtttggcag
    16621 aattggttga tgcagacctc ttcatcgttt tgacaggtgt agattatgta tttgttaact
    16681 acaacaagcc aaaccaggaa aaattggaac atgtgaatgt tgcccagctg gaagaatata
    16741 tcaaacaaga tcagtttgca ccaggtagca tgcttccaaa agtagaagca gctatcgctt
    16801 ttgtcaatgg tcgtccagaa ggaaaagcag ttattacttc ccttgaaaat ctaggcgcct
    16861 tgattgaatc tgaaagcgga acaattattg aaaaaggata agttgtttta ctaataagat
    16921 gtattctatt tctagtatct ttatatcaaa ttagaaatta ttc
//
LOCUS       DAVLYV010000037        16869 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLYV010000037 DAVLYV010000000
VERSION     DAVLYV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16869
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.037"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            113..316
                     /locus_tag="VYO70_001208"
     CDS             113..316
                     /locus_tag="VYO70_001208"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713442.1"
                     /translation="MISGIVIKKNRKTEEKQDEYKNEFGRKGSTCNGTTAESCLEEAY
                     EVIKDRKGKMIDGVFVKEEYYGE"
     gene            319..768
                     /locus_tag="VYO70_001209"
     CDS             319..768
                     /locus_tag="VYO70_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713443.1"
                     /translation="MPNFAEGTIKLRGHAENIKSALKYMFEAAGDVTIEEDTDGELII
                     FTSSNSYFYINGTKRAFIDGESFEIHLDDDFLIIELNNFTQAWRAIPDNYIEISEKFN
                     VDIKIFTFEQGLEFTQEIEISKGKILKDIVLKYGDYRWEVAFSNLGG"
     gene            787..1176
                     /locus_tag="VYO70_001210"
     CDS             787..1176
                     /locus_tag="VYO70_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713444.1"
                     /translation="MIPKFRVWHHELGRLMSVECMFFQDSEIEEFELNDTLMNDYITA
                     YPDEIELMQSTGLKDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTFIGGMPAVYLKG
                     LGDGYAWTEYEEIIGNIYENPELLEDK"
     gene            1176..1412
                     /locus_tag="VYO70_001211"
     CDS             1176..1412
                     /locus_tag="VYO70_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HEV1713445.1"
                     /translation="MNPEIIDNINKPSHYQGANGLEAIDVVHNFVGSLSGASAFFWGN
                     AIKYMLRFQKKNGLEDLKKARKNLDWLIEEMEHE"
     gene            1409..1567
                     /locus_tag="VYO70_001212"
     CDS             1409..1567
                     /locus_tag="VYO70_001212"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713446.1"
                     /translation="MNTHCMKATPLLPWGRLRKSAKKQELLNGASISEFAIEDICTFF
                     GIEIQERK"
     gene            1779..2180
                     /locus_tag="VYO70_001213"
     CDS             1779..2180
                     /locus_tag="VYO70_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1713447.1"
                     /translation="MKKLSDEELKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSGP
                     TVGISKPTETIAIRIADDPTLKFLEGFKGIINKLLSNLVDEDMEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAILKGEL"
     gene            2368..2910
                     /locus_tag="VYO70_001214"
     CDS             2368..2910
                     /locus_tag="VYO70_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1713448.1"
                     /translation="MEFVSPIKDNDDIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            3287..3607
                     /locus_tag="VYO70_001215"
     CDS             3287..3607
                     /locus_tag="VYO70_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEV1713449.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            3744..4136
                     /locus_tag="VYO70_001216"
     CDS             3744..4136
                     /locus_tag="VYO70_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HEV1713450.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYANLGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            4129..5859
                     /locus_tag="VYO70_001217"
     CDS             4129..5859
                     /locus_tag="VYO70_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEV1713451.1"
                     /translation="MGNLDKAKEYAQHVLTQREEHCEENILAAERFFRDLENPAFEMD
                     VDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVDYLDENILKMANPSWGVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYN
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     SMRERGFKIQAVGMDKKFGREFLAKMKKAKFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFSCIQALANLG
                     KGGDVMRFFD"
     gene            5867..6085
                     /locus_tag="VYO70_001218"
     CDS             5867..6085
                     /locus_tag="VYO70_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713452.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            6103..7305
                     /locus_tag="VYO70_001219"
     CDS             6103..7305
                     /locus_tag="VYO70_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEV1713453.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCKHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDDVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            7289..7864
                     /locus_tag="VYO70_001220"
     CDS             7289..7864
                     /locus_tag="VYO70_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEV1713454.1"
                     /translation="MQKRNSYRATQFQTREEESGDLVLSGYFIKFDEETELWRGYHEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFKL"
     gene            7861..9033
                     /locus_tag="VYO70_001221"
     CDS             7861..9033
                     /locus_tag="VYO70_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV1713455.1"
                     /translation="MNKALIFGARMRAKATKVVELEETIEELNKRSVVELEKLDRAKN
                     DEEVSAVEKTVDGLQREIEEKEAEKVQLENEIDELDKQIKEQNRKAPTYPSQEKRGGQ
                     KLEQRDAIAKYIRTGQTRDIVGLKTTDSGSAALIPTEVLKPHFVNKTRNPLLDLVERV
                     KVNSGSGKYPVIKKTDGVMVSTDELKSNPELGKPAISEIDYSIKTYRGYVPVSQEMID
                     DADYDIMSIVEDEVFNQGENTELSLVTAVLKTATQADAAGFDGIKDIYNKKLKSIYKA
                     SIVVTKSMFAALDKVKDKDGRYMLQTDVASPTGYSFGGKTIYKVDDTVFGNEGDMKFF
                     IGDVTEFVKEFDRSQVSVKWVNNDIYGQLLGLFIRLDIKKADEEAGFFGTYTDVVA"
     gene            9045..9335
                     /locus_tag="VYO70_001222"
     CDS             9045..9335
                     /locus_tag="VYO70_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713456.1"
                     /translation="MSYKVIRPFKDLADPENHDYAVGDIFPREGYEPTDSFTNGLLTG
                     ANTAGSIFLEVLGYDEPKKPAPETKEVKEEPAVEQEETVEETAEESAEEVEE"
     gene            9338..9619
                     /locus_tag="VYO70_001223"
     CDS             9338..9619
                     /locus_tag="VYO70_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEV1713457.1"
                     /translation="MDQGQLLELLKLKLGISTDLRDKPLKKIISSVITELTDNLGIEL
                     VGERADHEMFIVDYAAYRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            9606..9905
                     /locus_tag="VYO70_001224"
     CDS             9606..9905
                     /locus_tag="VYO70_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEV1713458.1"
                     /translation="MWNHEITLISKKVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEVLK"
     gene            9902..10249
                     /locus_tag="VYO70_001225"
     CDS             9902..10249
                     /locus_tag="VYO70_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEV1713459.1"
                     /translation="MSNDLADLIAKELAAYSDEVTEEVDKIAEQVTDETVDELKETSP
                     KRYGKYRRSWKKKKLANGSFVVFNAVASLTHILENGHLSRNGGRVAGIVHIKPAEEKA
                     IQNFEKRIKEIGK"
     gene            10246..10569
                     /locus_tag="VYO70_001226"
     CDS             10246..10569
                     /locus_tag="VYO70_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713460.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKIGNAPDLPYLVYYESSPVINS
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWANHKLFFEVQEETFIETERLYVKSY
                     TVYLY"
     gene            10581..11159
                     /locus_tag="VYO70_001227"
     CDS             10581..11159
                     /locus_tag="VYO70_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1713461.1"
                     /translation="MTQENKVTFGLENVHIAPIKTLAADGVITYGDVFRFPGAIELIL
                     DTKGETTPIKADNKDYHFMNSNEGYEGKLKIPHIIDEFATKILGEIKDPQTGVMTEKA
                     DASLTEFAMMFQFEGDKNKTRYVMYYCFASRPSLGSKTKNGTSTNERELSFKASPRPL
                     DTVVKRSITSADDKDAYDNWFKKVYEPTAVAA"
     gene            11171..11590
                     /locus_tag="VYO70_001228"
     CDS             11171..11590
                     /locus_tag="VYO70_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713462.1"
                     /translation="MRKIVLVGDQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATDIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     VFPIQEVGPVLMEMLNASMTTKKHQMNQNQLAKKSSQ"
     gene            11868..14618
                     /locus_tag="VYO70_001229"
     CDS             11868..14618
                     /locus_tag="VYO70_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEV1713463.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMVKVTSSFK
                     LQESALGSNASEAERNALAQKKIGAQSEIVSKQISNLEQQLEITKKEFGENSTQANKM
                     EAELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAQALDLTSRATRAAADGAAFYDKSIESVSESLQSFLKGNFANDAALGI
                     SATETTRNAAANKLYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFSSLPAPVKDFIVILGVV
                     LTIVGALAPIFLTLQAVFMSSFGAMIAAALPIIGIISGVVVAIAAIVAIVKYLWETNE
                     GFRDAVTTVWNAILEVINAVVSEISNFVMSIFGTVVTWWTENQELIRTSAETVWNAIY
                     TVISTILDILGPLLQAGWDNIQLIITTTWEIIKIVVETAINVVLGVIQAVMQIITGDW
                     SGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNILNGISGTVSNIWNSIKDTVSNV
                     LNAISSTVSSVWEGIKSTISSAINGARDAVSSAIEAIKGLFNFNISWPHIPLPHFYVS
                     GSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLNDKT
                     LGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRKTQLRGG
                     FT"
     gene            14615..15337
                     /locus_tag="VYO70_001230"
     CDS             14615..15337
                     /locus_tag="VYO70_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1713464.1"
                     /translation="MIKHNELVIDGVRTSSFPFKVIVHDSPSIALGESKTALLEHGGI
                     SGAIVQTNKHRELVKKTYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVSDLEEVQPGLYMTKATFTCHPTKHFKVTDTQRLTRSGTLTVQGSALAFPKITIVG
                     QSASETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            15338..>16869
                     /locus_tag="VYO70_001231"
     CDS             15338..>16869
                     /locus_tag="VYO70_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEV1713465.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKEIMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVEYNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGMALSRMVQNTKTALGDFSFNSNIQDRRTFNTTETETLYSILLDGKHSIVGT
                     WEGELVRDNFAITVKKSRGENRGVVITTHKNLKNYQRTKNSQNVVTRIHAKSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKTVEELQKWAQSKFSNEGIDKVSDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDAFKKAIAYEFDALKEEYISLTFDDKAGIGG
                     SRASGGLSSAADAILVVTESAQEIALDKALQNADLDFDHKAGLLRQEISDDIELAKAK
                     AEEVKRELSDTINQRFNSFDNGPLKETKRKAEEALRQAGASSSLAQEAKRIGLDSVAR
                     LEAFKSQTTSAQTALSGDLDALKRTIVNDIRPKQAQAEAEIAKQAEALSRTKNELAGA
                     STL"
BASE COUNT         5509 a         2908 c         3903 g         4549 t
ORIGIN      
        1 cgtaaagaac tagaagacgc aaacttcggc tgggtgtttg attgttcagg gattgagatt
       61 gaggaggttg agtatgatac aaatggggaa atgccaattt ctaaaaatct agatgatttc
      121 tggaattgtt ataaaaaaaa atagaaaaac ggaggaaaaa caagatgaat acaaaaatga
      181 atttggaaga aaaggttcaa cttgcaatgg cactacagct gaaagttgct tagaagaagc
      241 ttatgaagtt atcaaggacc gcaagggtaa gatgattgac ggggtgtttg ttaaagaaga
      301 gtattatgga gaataaagat gccaaatttt gcagaaggaa caattaaact aagaggacat
      361 gcagaaaata ttaaatcggc tttgaaatat atgtttgaag ctgctggtga tgtcacaatc
      421 gaagaagata cagatggtga gctaatcatt ttcacttcat caaactctta tttttacatc
      481 aatggcacaa aacgtgcttt tatcgacggt gaaagttttg aaattcatct tgatgatgat
      541 tttcttatca tcgagcttaa taattttacg caggcatggc gcgctatccc agacaattac
      601 atagaaatct ctgaaaaatt taatgttgat attaaaatct ttacatttga acagggcttg
      661 gaattcactc aagaaatcga aatttcaaaa gggaaaattt taaaagacat tgtattgaag
      721 tatggtgatt atagatggga agtcgcattt agcaatttag ggggatgaac agaaggagga
      781 tttggcatga taccgaaatt tagagtgtgg catcatgaat taggtagact gatgtcagtc
      841 gaatgtatgt tttttcagga tagcgagatt gaagaatttg agttaaacga tactttaatg
      901 aatgattaca ttacggctta tcctgatgaa atcgaactca tgcaatcaac aggactcaaa
      961 gacaagaacg gcaaggaggt attcatcggt gacatcgtta aatgtacaag aggatgtctc
     1021 catgaagtat atttagaaaa agaatacggt ggtacattca taggtggaat gcctgctgta
     1081 tacctaaaag gacttggaga tggatatgcg tggacggaat atgaggaaat catcggcaac
     1141 atctacgaaa atccagagct tttggaggat aaataatgaa cccagaaata attgacaata
     1201 taaacaaacc aagccactac caaggtgcaa acggtcttga ggctatcgat gttgtgcata
     1261 acttcgttgg gagcctttcc ggagcgtctg ctttcttttg gggcaacgca atcaagtata
     1321 tgttacggtt tcaaaagaaa aatggtcttg aagacctgaa gaaagctaga aagaatcttg
     1381 actggctgat tgaggagatg gagcatgagt gaatacgcat tgtatgaagg cgacaccttt
     1441 attaccatgg ggacgcttgc ggaaatcagc aaagaaacag gaattgctga acggtgcaag
     1501 tatttcagag tttgccatcg aagatatctg tacgtttttt gggattgaga tccaagaaag
     1561 gaaataaaag tcaatgaaca aaagaattaa gaagaagata gctaaacggc agatacaaga
     1621 aaagcaagaa gaattataca aacaactaca gaaattaagt ccggaagaaa ttgaagctat
     1681 aactaaaatg attaaccagt tagtttctaa tattcgcaag gctttatctc aaatatttga
     1741 taacttgttt acatttttta aaaacttgga ggtggaaatt gaaaaaattg agcgacgaag
     1801 aactcaaaac gttagacaga gaacttttca aatttcaaaa cattcaacgc acaatagact
     1861 taagaaggct agaattagaa accagaaacc cagatgctca aagtgggcca actgtaggaa
     1921 taagcaaacc taccgaaact atcgcaatca gaattgcaga tgatccgact ttaaaatttc
     1981 ttgaaggatt taaagggatt attaacaaac tcttgagtaa tctagtggat gaggatatgg
     2041 aaatctttaa tctacgctgg agataccctc aactgagatg ggaagaaata gcagaacaga
     2101 aattcatgag caaagctaca atctatcgac gtagaagaat tatcttagaa cagtacgcta
     2161 tactgaaagg tgagttgtaa ataagattga gacaaaagac atcttgaagt ctcacaaaaa
     2221 aaggtctatt atgatagcat gaacttctga aacaaaaaca caaatcacat gttggagtca
     2281 tcctattttt acagaaaagt tgtttaacag aggaacatca tgagtcagca accagctggc
     2341 tttttgtttt ttagaaagga ggcagttatg gaatttgtat caccgataaa agacaatgat
     2401 gatattcaag ctatgaaaga ttatctcaga gaatggaatg agatgtatta tatgctattc
     2461 atcacaggcc tgaatactgg cttacgagtc ggagatatac ttaccttgaa agttaaagat
     2521 gttcaaggtt ggcacatcaa actgagagaa cggaagactg gcaagcagat aacaagacgg
     2581 atgacaaaag aactcaagaa agaaatgagg agatatgttg aaggcaaacc atttcatcat
     2641 ttcttattca agagtaggca aggtcagaat aaagcgatca ctcgtgagcg agcctatcaa
     2701 atcatacatg aagcagctga agaacttggc attgataatg ttggcacaca tacaatgcgc
     2761 aagacattcg gctataaata ttacaacaag acaaaggacg taggaacatt acagaaaatg
     2821 ttcaatcact catcacctgc aatcaccctg agatacatag ggatagagca agcagagctt
     2881 gatgacgcac tacggaactt tgtcatttaa ttttttagat attactttca cataatgagt
     2941 taagcataaa ctaaaaaaat gaaactcttc aaaacccatg cttagtaagg gtttgagatt
     3001 cagagtgagt ttaacaaaat ataagatatg tgaaagtgag ggataaaatt ggtatagtta
     3061 caagaggtga aaaatggtaa aagaataccg tatttgtaaa tggaatgcat ataccgttaa
     3121 cagccatact agtaaaatgg aataattttt ttaaagaatc agaatgagac aaaagacatc
     3181 ttgaagtctc acaaaaaaag gtttattatg gtagcataga tttcttgtat gaggagggga
     3241 taggtcaaag gcctgtccct tttagcattg agaaaggagg tttgagatgt ataacaaacc
     3301 tatcagacca tccttgaaat ctaagaagtg ggagaagttc cgtgatagga taatgcgtaa
     3361 gcatgattat ctttgccaag aaagtttgcg ttacggaatt tctgttcaag cagaaatggt
     3421 acatcatatc tttcctgtgt ctgaatatcc tgagcttgaa tttgttgaat ggaattgctt
     3481 gccattgacg aataagaaac ataatacgtt tcatgataga gtgaacgata gagtaatcaa
     3541 ccaaggattg tattggcaga aaaaaagaaa aaaagaattt ttaaattttt tcaaaaatga
     3601 aaaatgaaaa tttttaatcc cccctctttt tgaaaaatca ttttggccag tagggtaccg
     3661 gtgaagggaa ctttttccaa gtcgggggcc ttcaaacaaa aagggggtaa aaactaagcg
     3721 attttgacga aaggaggtag tttttggcta aaccaattac agcaaagtcg attaagtcaa
     3781 aagtggtcaa gcagatgaaa gacttgggaa cttatcgtaa agagttcgaa atgatcattg
     3841 acattttcgc aggtatgtta taccagtatc agaaacttgc tcaagattat gctaacttgg
     3901 gttatccagt aacagacacc tacgtcaata aggctggtgc tgaaaatgag cgcaaagttc
     3961 caatcttgac agcgatggaa attttgagga aagacatact cagctactct aatcagttga
     4021 tgatgaatcc taagtcgctc ggtgaggtag tagaacaaga gggtgagtca gttcttactg
     4081 aggtcctgaa gttcaagaac gaaatcaaga agaagcgagt gactggcaat gggtaatctt
     4141 gataaagcaa aagaatacgc tcaacacgtc ttaactcaac gagaagaaca ttgcgaagag
     4201 aacattcttg ctgctgaacg ttttttccgt gatttagaaa atccagcgtt tgagatggat
     4261 gtggatatgg tggattttgt tattcacttt atcgagaacg tgatagttca tcagcagggc
     4321 gatgatatgt ttgcggtatc tatccgtaac aagccattgc ttttgcaacc gtggcaacat
     4381 ttcgttgtag ttaatctgtt tggtttttac tacaagggta cgaatgagcg cagattcaaa
     4441 gaagcgctta tcatgcttgc tcggaagaat gggaagacct cgtttactgc tgcaatcgca
     4501 cttgcttatc agatattaga tacagatagc ggttccaaat gctatatcgt tgctaactca
     4561 gtcaagcaag cgatggaagc ttttggtttt ttaagattca acgttgaacg ctggaacgat
     4621 aagaacattc gtatcaagga taataaccaa gaacactcta tcaccgccaa ttttggtgat
     4681 gaaggttctt tctttatcca ggctttagcg aatgatgaga gccgtctgga ctctttgaac
     4741 ggaaatgtta tcatcttgga cgaagctcac acaatgagga acagtaagaa acatggtctt
     4801 atgaaaaaaa caatgtcagc ataccgtaac agtatgcttt ttgttatctc tacagctggg
     4861 gatattccta ccgggttcct tgctaatcgt ttgaaatact gtcaaaaggt gctcaagcaa
     4921 ttggtcactg atgattcatt tttcatcttc atctgcaagg ctaatcaatc tgctgatggg
     4981 gatgtagtgg actatctgga cgagaacatc ctcaagatgg ctaatccgtc atggggggtc
     5041 acggtttcgc tcaaggctct caaggaagaa gcagagcagg ctatgaatga tcctcagaca
     5101 agaaatgagt ttttcaataa gaccttgaat atctttacaa actctatgaa cgcttatttc
     5161 aatccggatg agtttattgc gtcggatagt tgttacgatt ggagtctaga agagctggca
     5221 cgcttgccaa ttcgttggta tggtggtgcg gacttgtcaa gattgcacga cttaacagca
     5281 gctgctctct atggtgtcta taatgatggt gagaaagacg ttgatatctg tatcacacat
     5341 gctttctttc ctcggattaa tgctcagaag aaggctaacg atgatgggat tccacttttt
     5401 ggctggcagt ctgatggttg gttgacgatg agcaacactc caacggtact ctatgacgat
     5461 atcgtcaaat ggttcatcag tatgcgtgag cgtggattta aaatccaagc tgtggggatg
     5521 gataagaagt ttggtcgcga gtttttagct aagatgaaaa aggctaagtt caagatgatt
     5581 gaccagcctc agttattcta tctgaaatct gaggggttca gacggattga gttcaaagtc
     5641 aagaacaagg aattttacta tcttcattct gatgcttatg aatactgtgt gagcaacgtt
     5701 agagcaattg aaaaggtgga tgacgctgtg caatatgaaa aattagatgg agacggtggg
     5761 actgcaagga ttgacttgtt tgatgccagc gtcttttctt gtatacaggc tcttgctaat
     5821 cttggcaagg gtggtgatgt gatgagattc tttgattagg tgaattatga atgaaatagt
     5881 attatcagaa catgaaatta acttgctgat caacaaaggg cgagttaaag taattttaaa
     5941 cggggaagta gtaactattc gtcaaagaca tatgaaaaat ttgatggctg aaacagtaaa
     6001 atgggaaaaa caggtaattg atgtcagtca gaatatcgta agaaataaac actttgattc
     6061 actttttcaa aatacttttc gttagaaagg aggtgaggaa acatgggtat ttttgaaaag
     6121 ttttggaaac gaaacaagcc aagtaagtca atcaacatgc tgagtcattc agatttaggg
     6181 ttgtcaaacc tgatggactc gtatgtacct ttggccagaa atccagatgt ggtgacagcg
     6241 gttaataaga ttgctgattt ggtctctaat atgaccatcc acttgatgga gaatacagat
     6301 aaaggtgata tcagaatccg tgatgggctt gctagaaaga ttgatatcaa tccgtgtaaa
     6361 cacatgacaa ggaagtcatg gattttcaag attgtgcgcg atttgctttt atatggcgat
     6421 gggaactctg tcctacatgt ggaatatgaa cctgttacgg attatatttc taatctaaga
     6481 ccatttccga tgagagaggt ttcattccaa acagataagg attcctatgt aatctcattt
     6541 aggggtgaag aatattcccc tgatgacgta gtccacttcg tcatcaatcc agatccagat
     6601 attccataca ttggtactgg ttttagggtg acgttgacag atgtggttca aagtttgaac
     6661 atggctacca agactaaaaa aagctttatg aacgggaaga atattcctag tcttatcgtt
     6721 aaagtagact cgtctagtgc tgaactagat tcggagcaag ggcgtgagcg tatcgctgag
     6781 aagtatttga gtactagcag ggttggcgct ccatggattg ttccagaggc attgctggac
     6841 attcagcagg taaaaccgct tagtctaacg gacatcgctt taaacgagtc tgtggaattg
     6901 gataaaagaa cagttgcagg tctattagga gtacctgctt ttattttggg tgtaggagag
     6961 ttcaacaaga cagagtataa caactttgta aatacgactg tcatgagtat tgctaccact
     7021 attactcaaa cactaaccag ggacttactt ttgtctagca atcgttactt caagctaaat
     7081 cctcgttcac tcttctctta caacattacg gagttgtctg ctgttgctca acaaatggca
     7141 aacagtgctg caatgcgtcg taatgagtgg agggattggc tagggatggc tcctgatcct
     7201 gagatggaag agttgattgt tcttgaaaac tttctccctc aggagaaact aggagatcaa
     7261 aataaactga agggaggtga ggaagagaat gcaaaagcga aatagctatc gtgccactca
     7321 atttcaaacg agagaagaag aatctggaga cttggtgttg agtggctatt ttataaaatt
     7381 tgatgaagag acggagttgt ggcgtggtta tcatgaagta atcaagcgtg ctggtgttga
     7441 gaaagctgtc acagacgctg atatcagagc tttatttaac catgatgata gccttgttct
     7501 cggtcgaaca ggtaacggaa ctctgacact gggtgttgat gatgttggtc tttttggaga
     7561 tatcatcatt aacaaggatg atcctcaagc tgttggggcc tatgcccgtg tcaagcgtgg
     7621 agatgttata gggtgtagct ttggctttat accggtaaaa atcgaaacag aagaacgtga
     7681 agatggttca tatttggaca ctgtcttaga gctagaaatc tttgaagtga gtccatgtac
     7741 tttcccagcc tatccacaaa cggaaattgc tgcacgacaa aaagacttcg aaagtcagaa
     7801 gcgtgctaat cgtgaagcgc tagacaagcg caagaaagaa attaaggaga aatttaagct
     7861 atgaataagg cattaatctt tggtgctcgt atgcgagcaa aagcaactaa ggtagttgag
     7921 ttggaagaaa ctatcgaaga attgaacaaa cgttcggttg ttgagttaga aaagttggat
     7981 cgtgctaaaa atgatgaaga agtttcagca gttgaaaaga ctgtagacgg tcttcaaagg
     8041 gaaattgaag aaaaagaagc tgaaaaagta cagcttgaaa atgagatcga tgagttagat
     8101 aaacaaatca aagagcaaaa tcgtaaagca ccaacttacc caagtcaaga aaaacgtgga
     8161 ggacagaaat tggaacaacg tgacgcaatc gctaaataca ttcgtactgg tcaaactcgt
     8221 gacattgtag gcttgaaaac tactgattca ggaagcgcag ctttaatccc tactgaagtt
     8281 ttgaaacctc attttgttaa taaaacacgt aatccacttt tggatcttgt ggaacgtgtg
     8341 aaagttaata gtggatctgg taaatatcca gttatcaaga aaacggatgg tgtaatggtt
     8401 tcaacagatg aattgaaatc aaatccagaa ctcggaaaac cagcaatcag cgagattgat
     8461 tattcaatca agacttaccg tggatatgtc cctgtgtcac aagaaatgat tgacgacgca
     8521 gactatgaca tcatgtccat tgttgaagac gaagtgttca atcaaggtga aaacactgaa
     8581 ttgtcattag ttacagctgt cctcaaaaca gctacccaag cagatgcggc tggatttgat
     8641 ggtattaaag atatctacaa caagaagctt aaatcaattt ataaagcaag catcgttgta
     8701 actaagtcaa tgtttgctgc gcttgacaaa gtgaaggaca aagatgggcg ctacatgctt
     8761 caaactgatg tggcttcacc tactggctat tcatttggtg ggaaaacaat ctacaaagta
     8821 gatgacacag tgtttggaaa cgaaggagat atgaaattct tcatcggaga tgttactgag
     8881 ttcgtcaaag agtttgaccg ttctcaagta tccgttaaat gggtgaacaa tgacatttac
     8941 ggacaattgc ttgggctttt tatccgtttg gatattaaga aagcagatga agaagctgga
     9001 ttcttcggaa catatactga tgttgtagct taaggaggta gcgtatgagc tataaagtaa
     9061 tccgtccttt caaggacttg gctgatcctg aaaatcatga ctacgctgtt ggcgatatct
     9121 ttcctcgtga gggatatgag ccaacagata gctttaccaa tggccttttg actggcgcta
     9181 acactgctgg ctctatcttc cttgaggttt tgggatatga tgaacctaag aaaccagcgc
     9241 ctgaaacaaa agaagtgaag gaagagcccg cagttgagca ggaagaaaca gttgaggaaa
     9301 ctgctgaaga gtctgctgag gaagttgagg aatagacatg gaccaaggtc agcttttaga
     9361 gttgctgaag cttaagttgg gtatttcaac cgacttgaga gacaagccgt taaaaaaaat
     9421 catttcaagt gtcatcactg aattgaccga taatctcggt atcgagcttg ttggtgagcg
     9481 tgctgaccat gaaatgttta tcgttgacta tgccgcttat cgttatgagg gtggggtgga
     9541 tatgccacgt caccttcagt ggcgactgca taatttacag atagcatcaa agaaagaggt
     9601 caagaatgtg gaatcatgaa atcacactga tctccaagaa agtaacaggt aaggataagt
     9661 tactacaacc aatctctgaa gatgttgaag ttactctctt atgtcgcaaa aagagggtca
     9721 ctcgctctga attttatcag gcgaatcagg tagggcttaa accgagcttg gtcgttgaga
     9781 ttcgaaattt tgagtatgag aatcaagagc ttgcgaagtt cgaaggcaag caatatcata
     9841 tcttgaaaac ctatcctatt gattctgaaa ttttagagtt gactttgtca gaggtgttga
     9901 aatgagtaat gaccttgctg atttgatagc gaaagagctt gcagcttact ctgatgaggt
     9961 tactgaagaa gtggataaga ttgcagagca agtgactgat gagactgtgg atgagttgaa
    10021 agagacaagt ccgaaacggt acggaaagta tcgtagaagt tggaaaaaga agaagttggc
    10081 caatggctct tttgttgtgt tcaacgcagt tgcaagtctt actcacatac ttgaaaacgg
    10141 gcacctttca agaaatggtg gtcgtgtcgc tggtatcgtc cacatcaagc cagctgaaga
    10201 aaaagcaatt cagaactttg agaagcgaat caaggagatt gggaaatgaa gctatcagac
    10261 tttgctgcta ttttggaaca ggcaaacttg cctgtcactt atcgagcgtt taaaattgga
    10321 aacgctcctg acctacctta cctggtctat tatgaatcaa gtccagtcat caattcagct
    10381 gacaacacgg ttaatcatca gattaagagc gtgacagttg agctggcttt tgagagtaag
    10441 gatgaagatt tggaagaacg tctggaagag ctgtgggcaa accacaagct cttttttgaa
    10501 gttcaagaag aaacatttat cgagactgaa agactctatg tcaagtctta cacagtctat
    10561 ctatactaag gaggaatgac atgactcaag aaaataaagt aacctttggc ctagaaaatg
    10621 tacatatcgc acctatcaag acacttgcag cagatggagt tatcacttac ggcgatgttt
    10681 ttcgttttcc tggagcaata gagctgatac ttgatactaa aggggaaaca acccctatca
    10741 aagcagacaa caaggattac catttcatga attcaaacga aggatatgag ggtaaactta
    10801 aaatcccaca catcattgat gaatttgcga caaaaattct tggtgaaatc aaggaccctc
    10861 aaactggtgt tatgactgaa aaagcagatg cgagcttgac agagttcgca atgatgttcc
    10921 agtttgaagg cgacaaaaac aagactcgct atgtgatgta ctactgtttt gccagtcgcc
    10981 catctcttgg ctcaaaaact aagaacggga catcaaccaa cgaacgtgaa cttagtttca
    11041 aagctagccc gcgtccattg gatacagttg tcaagcgttc tatcacatca gctgatgaca
    11101 aggatgcgta tgacaactgg ttcaagaaag tctatgaacc tactgcggtt gcagcttaag
    11161 gagaaaatct atgcgtaaaa tcgttttggt tggtgatcag gagtatgagt tggggaccaa
    11221 cggctatact cctattgctt acaagcaaca atttgggaaa gattattttc aagatttgtt
    11281 ttcaatgttg aaaaatcaat cattcatgaa tgaattgaac aagctggaaa ctgacaaaga
    11341 gttgactgca actgatattg acatttcgat gctgtcagat tttgatatga catttttcaa
    11401 ccgtcttttt tggacctttg ctaaatctgc aaatcctcac atcaagcctt atgaacaatt
    11461 cttcatggaa atggaagtct ttccgattca ggaagttggg cctgtattga tggaaatgct
    11521 gaatgcgagc atgacgacaa aaaagcacca gatgaatcag aatcagctag cgaagaaatc
    11581 ttcacagtag aaacctactt atcttgctgt aaagaaactg gtctttctat tgatgatcta
    11641 aagcacattt caatcggaat ggctctggat tatcagacgg attatgtgaa tctacgaagc
    11701 gaggataagg gtggcgaacg gaaagccacg caagctgatt ttgacagttt ttaaagaaaa
    11761 atgagtgctg agagagcgat tctgagacca agttccttgg tctggctgca ttatcagtgg
    11821 tagaagttct ctcagcgctt ttctattttt ttgagaaagg aggaaatatg gcaggaaata
    11881 tcaaaggtat taaaattgaa atcgatggcg acacgcaacc cttacagaag gcgctgaaaa
    11941 atgtcaataa ggctgctact gatgcaagtc aggagttgag acagattgac aaagcattga
    12001 agtttgatac agggaacgta acgctcctga ctcagaagca agaggtcttg caaaagcaag
    12061 tttcgacgac caaagagaaa ctagaaacct tgagacaagc tcagtctcag gtggaacagc
    12121 aattcaaaaa tggcgatatc ggtgctgacc agtaccgtgc tttccaacgt gaagtagaag
    12181 ttactcaaaa cgtcctaaaa ggatatgagg gtaagcttgc aaatgtgaat caggcgctta
    12241 atgagaatgg gagtgctact cagaacaaca agaaccaatt aaaagagttg caaaatgagc
    12301 agagtcaact tgcttcagag atggtaaagg tgacaagctc attcaaactg caagaaagcg
    12361 ctttgggttc aaatgctagt gaagccgaga gaaatgctct tgctcagaaa aagattggtg
    12421 ctcagtctga gattgtaagt aaacagattt caaatctaga acagcaattg gaaatcacta
    12481 aaaaagaatt tggtgagaac tccacacaag ccaacaagat ggaagctgag ctaaatcagg
    12541 ctaagactgc ttttaatcat ctcaatgatg agatgaaggg aacaaagtct gctgctgata
    12601 gcactcaaga aagtttaagt gaaatctcaa gaaatttaag agcagaacta cttcaacagt
    12661 ttagtgagaa gttgagtgct atttcagaaa agcttgtgga agtaggaaaa gaagcgttag
    12721 aagcagctgc tcaaatgcaa gctagtaatg ctcaatttac taccgttttc ggagatatgg
    12781 aaacccaagc aagagaagcg ttgaatgcta ttggtcagga aatggatatt gtcccagagc
    12841 gtttacaagg gtcatttact cagatggctt catttgctaa aacttcagga ctagacactg
    12901 cgcaagcatt ggacctgact tcccgtgcaa ctagggcagc ggcggatggc gcagcttttt
    12961 acgacaagtc catcgagagc gtatcagaaa gcctacaatc tttcctgaaa ggaaactttg
    13021 ctaacgatgc cgctcttgga atctctgcga cagaaacgac caggaatgca gctgcaaata
    13081 aattgtacgg aaagtcattc aaggacttga gcgaagcgca gaagcaattg accttgcttc
    13141 agatggtcga agacggaaat aaactctcag gagctcttgg acaggctgca agagaatcag
    13201 acggcctaga aaacgtgatg gggaatctga aacaagctgg gaccaatgca ttatctgcta
    13261 ttggtcaacc tcttctagaa atgatgattc ctgtttttca aaccttggca acgattgtga
    13321 aaggtgtagc tgagctgttc agttccttac ctgctccagt aaaagatttc attgtcatct
    13381 taggtgtggt tttgacaatt gtaggagcct tagcccccat attcttaacc ctgcaagctg
    13441 tgtttatgtc ttcatttggc gcaatgattg cagcagcatt accaatcatt ggaattattt
    13501 caggagttgt agtggccata gcagcgattg ttgctattgt gaaatatctc tgggaaacta
    13561 acgaaggttt tcgagatgcg gtcacgaccg tttggaatgc gattcttgaa gttatcaatg
    13621 cagtcgtatc agagatttct aattttgtca tgagtatctt tggaacggtt gttacttggt
    13681 ggacggagaa ccaggaactt atcaggacaa gtgctgagac tgtctggaat gccatttata
    13741 cggtcatcag tacaatactg gatatacttg gccccttgct ccaagctggc tgggataaca
    13801 ttcaactgat cattacaaca acttgggaaa tcatcaagat cgttgttgag actgcaatca
    13861 atgttgtcct tggtgttatc caagcagtta tgcagatcat tactggtgat tggtcaggag
    13921 cttgggaaac tatcaaggga gtgttttcta ctgtatggca agctattcaa agcattgttc
    13981 agactatttt ctcagctatc cagagttata tttcaaatat tctcaacggc atttcaggaa
    14041 ctgtatcaaa tatctggaac agcatcaagg acactgtctc aaatgtgtta aatgctatat
    14101 ctagtactgt atcaagtgtt tgggaaggta tcaagagtac catttcaagt gctatcaatg
    14161 gtgcaaggga tgctgtttct tcagctattg aagccatcaa aggattgttt aacttcaaca
    14221 tcagctggcc acatatccca ctacctcact tttacgtgag tggttcggcc aatccattag
    14281 attggttgag tcaaggtgtt ccaagtattg gaatcgaatg gtatgccaaa ggcggtatca
    14341 tgacgaaacc aactatcttt ggaatgaatg gtaataacat aatggttggt ggtgaagctg
    14401 ggaatgaagc agtattgcca cttaacgaca aaacgcttgg agccatcggt cgaggtatcg
    14461 ctcaaactat gggtggaact tcaccaacca ttaacattac cattactggc aacactgtca
    14521 gagaagaagc cgacatcagt cgtattgctg atgaggtagc gcagaggatt gctgacgaat
    14581 tgcaacgtaa gacacaattg agaggagggt ttacatgata aagcataatg agcttgtgat
    14641 tgacggtgtg agaacatcgt cttttccgtt taaagtcatt gtccatgatt ctccttcaat
    14701 tgctctggga gagagcaaga cagctctttt ggagcatggt ggtatcagtg gagcaatcgt
    14761 tcagacgaac aaacataggg aattggtcaa gaaaacctat acgatttact tggtcaaacc
    14821 tactgaagaa cagatgaacc aatttatgag tctgtttatc cgtgaaaagt tctggctaga
    14881 gagtgagcga gtcaaaacaa ctcgtctttg gtgctataag gtcaatgtga gcgaccttga
    14941 agaagtgcaa cctggtcttt acatgaccaa agcaaccttc acttgtcacc ctaccaaaca
    15001 cttcaaagtc actgatacac agagattgac aagaagtgga actttaaccg ttcaaggttc
    15061 tgctcttgcc tttcctaaaa tcacaattgt tggtcagagc gcttctgaga cttcgtttac
    15121 aatcgctggt caggtcattc gtcttgaaaa gctctcagaa tcgcttgtga tggttaataa
    15181 tcctgacaat cctagcttta aaacgacaac agggaagcca gtcaaatggt caggggattt
    15241 tatcacagtt gatccagcga aagttaagaa tattggggtt gttttaggcc caggtattca
    15301 atcgattgaa atcgaaacgg tttgggggtg ggcataattg ctttatctac ttaataaaga
    15361 tgtaagaacc gttcgatgga acggggagcc acttcatgaa gcgacttcgg cgattgttaa
    15421 agagatcatg aatggtgatt tcaccttaac tgtgaaatat cccatttccg actctggtat
    15481 ttatcagctc atccaagaag atatgttgat aaaagcgccg actcctgttc ttggtgcgca
    15541 gctatttcgc attaagaaac ctgttgaata caatgatcat ctggaaatca cagcctatca
    15601 catttcagac gatgtgatgc aacgttctat cacaccagta agtgtgacta gtcagagttg
    15661 tggcatggct ctttctcgca tggtacaaaa caccaaaacg gctcttggag acttctcatt
    15721 caacagtaat atccaggacc gtaggacctt caatacgact gaaacagaaa ccctatactc
    15781 tatattgcta gacggtaagc acagcattgt tggtacatgg gaaggcgagc tggttcgtga
    15841 taactttgcg ataactgtca agaagagtcg tggggagaat cgtggtgttg ttattacaac
    15901 gcacaaaaat ctgaagaact accaacgcac aaaaaacagt cagaatgttg tcacaagaat
    15961 tcacgcaaag tcgactttta aacctgaagg tgctgaaaaa gaaacgacta tcagagtgac
    16021 tgttgatagt cctcttatca actcataccc ttatatcaat gaaaaagagt atgagaacaa
    16081 caatgctaaa actgttgaag agttgcagaa gtgggcacag tctaagttct caaatgaggg
    16141 aattgacaag gtctctgatg ctatcaagat tgaagcttat gaacttgatg ggcaagtggt
    16201 ccatatgggc gacacggtca atctcaagag ctggaaacat aatgtcgatg cattcaagaa
    16261 agctattgct tatgagttcg atgccttaaa agaagaatac atctctctga ctttcgatga
    16321 taaggcaggt attggtggtt ctagagcttc tggtggccta tctagcgcag ccgatgcaat
    16381 tcttgtagtg acagaatctg cacaagaaat cgcccttgac aaggctcttc aaaatgctga
    16441 cttagacttt gatcataagg ctggattgct tagacaggaa atttctgacg atattgaact
    16501 tgccaaggcc aaggcggaag aagtcaagcg agaactgtct gacactatca atcagcgctt
    16561 taatagcttt gacaacgggc cattgaaaga aactaagcgc aaggctgagg aagctttgag
    16621 acaagctggc gcaagtagct ctcttgctca ggaagccaag cggattgggc tggattctgt
    16681 tgctagactt gaagcgttta agtcgcagac tacgagcgca caaacggctc tgtcaggtga
    16741 cttggacgct ctgaaacgga ctatcgtgaa tgatattcga ccgaagcaag cacaggctga
    16801 agctgagatt gccaagcaag ctgaagcact tagccggact aaaaatgaac tggctggcgc
    16861 aagtaccct
//
LOCUS       DAVLYV010000038        16316 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLYV010000038 DAVLYV010000000
VERSION     DAVLYV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16316
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.038"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            94..1872
                     /locus_tag="VYO70_001232"
     CDS             94..1872
                     /locus_tag="VYO70_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1713466.1"
                     /translation="MSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIY
                     WNKKTNKYKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIG
                     RSAMVTKLLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYP
                     ELEPSKVGTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGK
                     TYLAKEIAKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKD
                     FCQKAKETQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSG
                     VPGWSLPSKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKF
                     VFIIDEINRGEISKIFGELFFSIGPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIG
                     TMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIEN
                     VQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAF
                     ELTNNEQKDQAVADDNEGDENDDADY"
     gene            1859..3175
                     /locus_tag="VYO70_001233"
     CDS             1859..3175
                     /locus_tag="VYO70_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1713467.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLVYLFPKYLQAAIRKGLYKEYHRFSHNDSNVKGV
                     IDVRSHLKKNLPFTGNIAYATREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLIFPSMEQSVNSEIGKV
                     AGYGAQLKEWSIRIPQNASFYSTFCKMMENSEENFKAIIDEEVGRK"
     gene            3350..4336
                     /gene="guaC"
                     /locus_tag="VYO70_001234"
     CDS             3350..4336
                     /gene="guaC"
                     /locus_tag="VYO70_001234"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1713468.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHNQGLIASISVGVKDYEY
                     DFVRQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPTKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            4400..4471
                     /locus_tag="VYO70_001235"
     tRNA            4400..4471
                     /locus_tag="VYO70_001235"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4433..4435,aa:Arg,seq:ccg)
     gene            4654..5352
                     /locus_tag="VYO70_001236"
     CDS             4654..5352
                     /locus_tag="VYO70_001236"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1713469.1"
                     /translation="MKELQTVLKNHFAIEFTDKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            5343..8882
                     /gene="smc"
                     /locus_tag="VYO70_001237"
     CDS             5343..8882
                     /gene="smc"
                     /locus_tag="VYO70_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1713470.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            8879..9673
                     /locus_tag="VYO70_001238"
     CDS             8879..9673
                     /locus_tag="VYO70_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1713471.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYRGQDLYEATMSREFYLTTFEKLKTSPYV
                     DINKLLLTGKKGAYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNENVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGIIMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            9673..10491
                     /locus_tag="VYO70_001239"
     CDS             9673..10491
                     /locus_tag="VYO70_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1713472.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAASDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            10495..11715
                     /gene="ftsY"
                     /locus_tag="VYO70_001240"
     CDS             10495..11715
                     /gene="ftsY"
                     /locus_tag="VYO70_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1713473.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAVLEESQDSEPAVGVSQEEIEELPEEEKLEHEGTIEENNSEVLEAERPQAEETVQ
                     EKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEELLIMSDVGVQVASNLTEELR
                     YEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNLTVMLFVGVNGVGKTTS
                     IGKLAHRYKRTGKKVMLVAADTFRAGAVAQLAEWGRRVDVPVVTGPEKADPASVVFDG
                     MERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKRVVPEAPHETFLALDASTGQ
                     NALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIPVKLIGFGEKIDDIGEF
                     NSENFMKGLLEGLI"
     gene            complement(11759..13246)
                     /locus_tag="VYO70_001241"
     CDS             complement(11759..13246)
                     /locus_tag="VYO70_001241"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1713474.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFDAKWTWQPDIAYRQDGRFE"
     gene            complement(13395..14135)
                     /locus_tag="VYO70_001242"
     CDS             complement(13395..14135)
                     /locus_tag="VYO70_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1713475.1"
                     /translation="MAKLKIDVNDLHKYYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(14135..16300)
                     /locus_tag="VYO70_001243"
     CDS             complement(14135..16300)
                     /locus_tag="VYO70_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1713476.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQSTKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
BASE COUNT         5093 a         2908 c         3616 g         4699 t
ORIGIN      
        1 gagttattaa ataaggcaac tgttggtgaa aagtttaatg ataaattaaa agaagagttt
       61 ctccaggaat ggcctttgga taggatttta acaatgtcaa tcgatgaata tgtaatagga
      121 aagggacagc aaaataagtc tttatgctac gctcttgaga agggaaaata caaaaatcta
      181 tttcttggaa tttctggtgg ctcagcttca aaatttggta tttattggaa taaaaaaaca
      241 aacaaatata aagatcaagc taataatgag atttcagagt tggatcagcg attttcaaaa
      301 ttaaaatcag atttgtatga aattatcaaa gaaggtattc gttttaactt tgaaaatcct
      361 atttttgata tgaaaagatc aacaaatgaa tttattggtc gttctgctat ggtgacaaaa
      421 ttactttgta tctattctga gggagatcct ttctttggtg taaatattaa tagtcagaaa
      481 gaattttgga accactttgt ttctcagaca aatcaaggtg gaccttatct gcaaaatcat
      541 aaaataattg aactggtgtc caaaacttat cctgagttgg agccatcgaa agtaggaact
      601 atgctttttg agtattctaa gctttttatg gaaaataagg aagacaatag tacaatggat
      661 tcatcaaaca attttcgtca tcaattaact caatctctat taaagtctcc aaaccttatc
      721 cttcgtggtg ctcctggcac aggaaagact tatcttgcta aagaaattgc caaagaatta
      781 acggatggca acgaagacca aatcggattt gtacaatttc acccatcata tgattatacg
      841 gattttgtag aaggtttaag accagtatca aatggggatg gagctattga gtttaggcta
      901 caggacggta tttttaaaga tttttgtcag aaagcaaaag aaacccaatt gattggagga
      961 caagataatt ttgatgaggc ttgggattct tacttagaat atataaatgt tgctgaagaa
     1021 aaagaatata taacaaaaac atcttactta tctgttaata gtagacaaaa tttgtcagta
     1081 aattatgata gtggtgttcc aggatggtca ctacctagca aatatgttta cgagttgtat
     1141 aaagataaaa attataataa gcaagaatac tacaaaagtg gtggaaaaac tgtcctagaa
     1201 acattgagaa agagatttgg tttgaaagac tatgtttccc caacagaaat tgatactgat
     1261 aagaaattcg tcttcatcat cgatgaaatc aatcgtgggg agatttctaa gatttttggc
     1321 gaactctttt tctctatcgg ccctggctat cgtggtgaaa aaggaagtgt ttctacccaa
     1381 tatgcaaatc tacacgaaac tgatgaaaag ttctatatcc ccgaaaatgt ttacatcatc
     1441 ggaactatga atgatattga tcgttcagtg gatacctttg attttgctat gcgtcgtcgt
     1501 tttcgttttg ttgaagttac tgccgagggt caagttggca tgttggataa agagctgaat
     1561 atccatgcag aagaagcaaa aattcgtcta agaaacttga acgctgctat cgaaaatgtt
     1621 caggaattaa acagtcatta tcatattgga ccaagttatt ttcttaagtt gaaggatgta
     1681 gattttgact atgaattact ctggtctgat tatattaagc ctctcctaga agactacttg
     1741 cgaggttctt atgatgaggt tgaaactttg gaaactttga aaaaagcatt tgaactgaca
     1801 aataatgagc aaaaagatca ggcagtagct gatgacaatg aaggtgatga aaacgatgat
     1861 gcggattact gataatcaac acaagattat taaagaaaaa tttgttgaag aatatcctaa
     1921 actaagcaat cttcttttag acagaacctt ggaaagttta tcccaagatg aacgtatttt
     1981 catttttcca aatgatttga ctcatactcc tgatttggat aaggaccaaa agatttttga
     2041 aacagtcaat cagaaaatca agacagggaa cgtgattggt tttcttggat atggtcagga
     2101 aagattaacg atttcctcac gattttctga tgagagtaat gaccattttt tgcattatct
     2161 cttaaacaag gttcttcata tcaatctcac tagtttagat gttgctttgt ctcgtgaaga
     2221 gaggctttat caacttttgg tgtatctctt tcccaagtat ctacaagctg ctattcgaaa
     2281 aggtctttat aaggaatatc atcgattttc tcataacgac agtaatgtta agggagtgat
     2341 tgatgtaaga agccatctca agaaaaatct tcctttcacg ggaaatattg cctacgcaac
     2401 gagagagttc acctatgata atcccctcat gcagttggtc cgtcacacta ttgaatacat
     2461 taagaatcag aaaagcattg gtcaaggggt actagataat ctctcaacta gtcgtgaaaa
     2521 cgtatctgaa atcgtgcgtg taacgccctc ttataaacta gctgatcgtg ctaagattat
     2581 tcggggaaat caatctaaac ctatacgtca tgcatacttt cacgagtaca gaaacttaca
     2641 agaactttgt ctgatgatcc taaaccaaga aaagcacggt ttagggtatc aagatcaaaa
     2701 aatctatggt attctctttg atgttgcctg gctttgggaa gagtatgttt acaccttgtt
     2761 gccaaaaggt tttgtacatc ccagaaataa ggataagacg gatggaattt cagtattttc
     2821 tgttgggaaa cgaaaagtat atccagattt ttatgacaga gaacgaaaga ttgttctaga
     2881 tgcaaaatat aaaaaactgg aattgactga aaaaggaatc aaccgtgagg acttattcca
     2941 gctgatttcc tattcttata ttttaaaagc tgagaaggct ggactgattt ttcctagtat
     3001 ggagcagtca gtaaatagtg aaataggaaa agtagctggc tatggagctc aattgaagga
     3061 gtggtctatt cgaatccctc agaatgcctc attctatagt acattttgta aaatgatgga
     3121 aaattcagaa gaaaatttta aagcgattat tgatgaagaa gtggggagaa aataatttcc
     3181 cctctttctt ttatttttaa cacaagtaaa acccgaacga tatattttaa ttcataaaat
     3241 aatttgacaa aaactgtact ttggtttata ataaattaag gaaacgtcgg aaaaggcgta
     3301 gtgatgcaag agcataggta ggtcattaca aaagaaacga gacatcgata tgttaaatga
     3361 atttccaatt tttgattacg aagatattca attgattcca aataaatgtg tcattaaaag
     3421 ccgtgcagaa gcggatacaa gtgtcacttt aggaaatcac acctttaaac tacctgttgt
     3481 gccagcgaat atgcagacga ttttggatga aaatgtagca gagcaactgg ctaaaggtgg
     3541 ttacttctac attatgcacc gttttgatga ggcaggacgc attcctttta ttaaacgaat
     3601 gcacaatcaa gggctcattg cttccatatc tgtcggtgtt aaggactatg agtatgattt
     3661 cgttagacag ctcaagactg atgctcctga gtatatcact attgatattg ctcatggtca
     3721 tgcggatagc gtgatttcta tgattcaaca catcaagaaa gaattgccag atacctttgt
     3781 cattgctgga aatgtgggaa caccagaagc tgtgcgtgaa ttggaaaatg ctggtgcgga
     3841 tgctactaag gtcggaatcg gtcctggtaa ggtttgtatc accaaggtta agactggttt
     3901 tggtacaggt ggttggcagt tggctgctct acgctggtgt gccaaggctg cgcgtaaacc
     3961 gattatcgct gatggaggaa ttcgtactca cggtgatatt gccaagtcta tccgcttcgg
     4021 tgctagcatg atcatgattg gttccctctt tgcaggacat attgaaagtc cagggaaaac
     4081 gattgaagtc gatggtgaac agttcaaaga atattatggt tcagcctcac aatatcaaaa
     4141 aggtgcttac aaaaatgtgg aaggcaaacg tatcttgctt cctactaaag gtcatctgca
     4201 agacacttta actgagatgg aacaagacct tcaaagtgct atttcttatg caggtggacg
     4261 tcaggttgct gaccttaaac acgttgatta tgtgatcgtg aaaaactcca tctggaatgg
     4321 ggatgcttcc cactaagacg gggtctttga ctaaaaaact tgttattaga gcaaatttct
     4381 gttataataa aacaagtttc cacccttagt gtaatggata tcacgtaaga ttccggttct
     4441 tgagatgggg gttcgattcc ctcagggtgg atgtaaacat tctaaaaagc ctttaatagg
     4501 gcttttttct ttacactact ctaaatttgc cactaaaact gagaatctct gtctaaagaa
     4561 ttcccaaatg ctttccagtc ccgttttaga gtgactcagg gggctttttt tgatataata
     4621 aaaaggactg ttatcagtta gaaagaggtt ggtatgaaag aattacaaac tgtactaaag
     4681 aaccattttg caatcgaatt tacagacaaa aagttactgg aaactgcctt tactcatacg
     4741 agttatgcca atgagcaccg cctcttaaaa atttcacata atgaacgctt ggaattttta
     4801 ggagacgctg ttctacagtt attgatttca gaatatctgt ataaaaaata tcctaaaaag
     4861 cctgaaggtg acctatcaaa actccgtgct atgattgtcc gtgaggagag tttagctggt
     4921 tttgcgcgtg attgccagtt tgaccagttt atcaagttgg gtaaagggga agaaaaatct
     4981 ggtggtcgca atcgtgacac cattcttggt gatgcctttg aagcctttct tggtgccctt
     5041 cttttggata aggatgtggc caaggtcaag gaatttatct atcaagtcat gattcctaag
     5101 gttgaagcag gcgaatttga gatgattaca gactataaaa cccatctcca agagttactt
     5161 caggtcaatg gtgatgtggc tattcgttat caggtgattt ctgaaacagg gcctgctcac
     5221 gataaggtct ttgatgtaga agttcttgtt gaaggtaaga gcatcggtca aggccaaggt
     5281 cgttctaaga aattagcaga gcaggaagct gccaaaaatg ccgttgagaa ggggctggat
     5341 tcatgtattt aaaggaaatc gaaattcagg ggttcaagtc ttttgctgat aagaccaagg
     5401 tcgtttttga ccaaggtgtg acggcagttg ttggacccaa tggatctgga aagtccaata
     5461 ttacagaaag tctgcgttgg gctttggggg agtctagtgt caagagtctc cgtgggggca
     5521 agatgccgga tgtcatcttt gctggaacag aaagtcgcaa accgctcaat tatgcttctg
     5581 tagttgtgac tctggataat catgacggat ttatcaagga tgcaggtcaa gaaatcaggg
     5641 tggaacgcca tatctatcgt agtggagata gcgaatacaa gattgacggc aagaaagtcc
     5701 gtctgcgtga tattcatgac cttttcttgg atacaggttt gggacgagat tccttctcta
     5761 ttatttccca agggaaggtt gaggagattt ttaattccaa gtctgaggaa cgacgagcta
     5821 tttttgaaga agctgctgga gttttaaaat acaagactcg cagaaaagaa accgagagta
     5881 aactgcagca aactcaggat aatctggacc gcttagagga cattatctac gagttggata
     5941 atcaaatcaa gcctcttgag aagcaagctg agaatgcccg taaatttcta gacttagaag
     6001 gtcaacgcaa ggctatttac ttggatgtat tggttgctca aatcaaggaa aataaggcag
     6061 aactagagtc gacagaagaa gagttggctc aggttcaaga actcttgatg agttattacc
     6121 aaaagcgtga aaaattagaa gaagaaaatc aaactcttaa aaagcaacgc caagatttac
     6181 aggctgaaat ggccaaagac caaggcagtt tgatggactt gactagtctg attagtgatt
     6241 tagaaagaaa attagcccta tcgaaactgg agtctgagca agtggccttg aatcaacagg
     6301 aggcacaagc tcgtttggct gctttggagg ataagagaaa ttcactcagc aaagaaaagt
     6361 ctgataaaga aagctcttta gctctgttag agggaaatct agtccaaaat aatcaaaaac
     6421 tcaatcgttt agaagctgaa ttgctggctt tctcagacga tcctgatcag atgattgagc
     6481 tcttacgtga acgctttgta gctcttttac aagaagaagc ggatgtctca aaccagttga
     6541 cccgtattga gaatgagttg gaaaatagtc gtcagctttc tcaaaaacaa gcagatcaac
     6601 tagaaaagct gaaagagcaa ttagctatag ctaaagagaa ggctagtcag caaaaggaag
     6661 agcttgaaac tgccaaggag caggttcaga aattattggc tgactaccaa gctattgcca
     6721 aggagcaaga ggagcagaaa acttcctatc aagctcaaca aagccaactc tttgaccgtc
     6781 tggataatct caaaaacaag caggccagag ctcaaagttt ggaaaatatc ctgagaaatc
     6841 atagtaactt ttatgcaggt gttaagagtg ttctccaaga aaaagatcgc ctaggtggga
     6901 ttattggtgc agtcagtgag catctgacct ttgatatgca ttatcaaact gccctagaaa
     6961 ttgcgcttgg agcgagtagc caacatatca tcgtagaaga tgaagagtcg gcaaccaagg
     7021 ctattgactt ccttaaacgg aacagagccg gtcgtgcaac ctttcttcct ttgaccacta
     7081 tcaaggcgcg tacgatttct agtcagaacc aagatgctat cactgtaagc ccaggtttcc
     7141 ttgggatggc agatgagttg gtgacttttg atactagact ggaagccatt ttcaagaact
     7201 tgctagctac gacggctatc tttgatgccg tagaacatgc gcgtgcagca gctcgccaag
     7261 ttcgttatca ggttcgtatg gtgacattgg atgggacaga attacgcacg ggtggttcct
     7321 atgcgggtgg tgccaatcgc caaaataaca gtattttcat caagccagaa ctggagcaat
     7381 tacaaaaaga aattgctgca gatgaagcaa gcttgcgttc agaagaagcg gctttgaaga
     7441 ccttacaaga ccagatggct acattgacag aaagattaga agtcatcaaa tctcaaggag
     7501 aacaggcacg tattcaggag caaggcttgt ccctcgctta tcagcaaact agtcagcaag
     7561 ttgaagaact ggaaactctt tggaaactcc aagaagagga aatagatcgt ctttccgagg
     7621 gagattggca agcggataag gaaaaatgcc aagatcgtct tgctgcaatc gccagtgaca
     7681 agcaaaatct ggaagctgag attgaagaga ttaagtctaa caaaaatgcc atccaagaac
     7741 gctatcaaaa cttgcaggaa gagctagcgc aagctcgttt gcttaagaca gaactgcaag
     7801 ggcaaaaacg ttatgaagtt gctgatattg aacgcttagg caaggaattg gacaatcttg
     7861 attttgaaca agaggaaatc cagcgccttc ttcaagaaaa ggttgataat cttgagaagg
     7921 ttgatacaga attgctcagt caacaggcgg aagaagccaa aactcagaaa acaaatctcc
     7981 aacaaggttt gattcgcaag cagtttgagt tggatgatat tgaaggtcaa ctggatgata
     8041 ttgctagtca tttggatcag gctcgccaac agaatgaaga gtggattcgc aagcaaacac
     8101 gtgctgaagc caagaaagaa aaggttagcg agcgcttgcg ctatttacaa agtcaattaa
     8161 cagaccaata tcagattagc tatactgaag ctctagaaaa ggcgcatgaa ttggaaaacc
     8221 tcaatctggc agagcaagag gtgcaggatt tagagaaagc tattcgctca ctggggcctg
     8281 tcaatataga agctattgac cagtacgaag aagttcacaa tcgtctggat ttcctaaata
     8341 gccaacgaga tgatattttg tcagcgaaaa atctgctcct tgagaccatt acagagatga
     8401 atgatgaggt caaagatcgc tttaaatcaa cctttgaggc cattcgtgaa tcctttaaag
     8461 tgaccttcaa acagatgttt ggaggaggtc aggcagactt aatcttgact gagggagact
     8521 tgttgacagc tggggttgag atttctgttc aacctccagg taagaaaatc cagtcgctta
     8581 acctcatgag tggtggtgaa aaagccttat cagctcttgc tttgcttttt tctatcattc
     8641 gagttaagac tatcccattt gtcatcttgg atgaggtaga ggccgcgctg gacgaagcca
     8701 atgttaaacg ttttggggat tacctcaatc gatttgacaa ggacagccag tttatcgtcg
     8761 taacccaccg taagggaacc atggcagcgg ctgattctat ctatggagtg accatgcaag
     8821 aatcgggggt ctcaaaaatt gtttcggtta agttaaaaga tttagaaagt attgaaggat
     8881 gacaattaaa ctagtagcaa cggatatgga cggaaccttc ctagatggga atggacgctt
     8941 tgatatggac cgcctcaagt ctctcttagc ttcctacaag gaaaaaggga tttactttgc
     9001 ggtggcttcg gggcgcggat ttctgtctct agaaaaatta tttgctgatg ttcgtgatga
     9061 cattattttt atcgcagaaa atggcagttt ggtagagtat cgaggtcaag acttgtatga
     9121 agcgaccatg tctcgtgagt tttatttgac gacttttgaa aagctgaaaa cttcacctta
     9181 tgtagatatc aataaattgc tcttgacggg gaaaaaggga gcttatgttc tagatacggt
     9241 tgatgagacc tatttgaaag tgagtcagca ctataatgaa aatatccaaa aagtagcgag
     9301 tctggaagat attacagatg acattttcaa atttacgacc aacttcacag aagaaacgct
     9361 tgaagctggg gaggcttggg tcaatgaaaa tgtccctggt gttaaggcca tgacaactgg
     9421 ctttgaatcc attgatattg ttctggacta tgtcgataag ggtgtggcca ttgttgaatt
     9481 ggctaaaaaa cttggcatca taatggatca ggttatggct tttggagaca atctaaatga
     9541 tttgcatatg atgcaggttg tgggacatcc tgtagctcct gaaaatgcac gacctgaaat
     9601 tttagaatta gcaaagactg tgattggtca ccataaggac cagtcggtta tagcttatat
     9661 ggagggctta taatggcaga tataaaatta atcgcattgg acttggacgg taccttgctg
     9721 actactgata aaaggctgac ggatcgcacc aaggcaactt tgaaagctgc gagtgatcgt
     9781 ggtatcaagg tcgtattgac aactggtcgt cccttaaaag ccatggactt ctttctccat
     9841 gagttaggga ctgacggcca tgaagatgag tataccatta cttttaatgg tggattagtt
     9901 cagaaaaata caggagaaat ccttgataaa acagtctttt catatgatga tgtggcacgt
     9961 ttgtatgagg aaacagagaa attatcactg cctcttgatg ccatctcaga aggaacagtt
    10021 tatcaaatcc aatcggacca agaaagtctt tatgccaaat tcaatccagc tttgactttt
    10081 gttccagttg actttgaaga cctgtctagt caaatgacct acaacaaatg cgtgactgcc
    10141 tttgctcaag aacccttgga tgcagccatt cagaagattt ctccagaatt gtttgaccaa
    10201 tatgaaatct ttaaatcacg tgaaatgttg ctagaatggt caccaaagaa tgttcataaa
    10261 gcaacaggtt tggcaaaact aattagccat cttggaattg atcaaagtca agtgatggcc
    10321 tgtggtgacg aggccaatga cctctctatg attgaatggg cagggctcgg tgttgccatg
    10381 caaaacgctg ttcctgaagt aaaggcagcc gcaaatgtag tgacgccgat gaccaacgat
    10441 gaggaagctg tcgcctgggc tatcgaagaa tatgtgctaa aggagaacta agatatggga
    10501 ttatttgacc gtctattcgg aaaaaaagaa gaacctaaaa tcgaagaagt tgtaaaagaa
    10561 gctctggaaa atcttgattt gtctgaagat gttgatccta ccttcacaga agttgaagaa
    10621 gtttctcaag aagaagctgt gcttgaagaa agtcaggatt cagagccagc tgtaggggtt
    10681 tctcaagaag aaatagaaga attaccagag gaagagaagc ttgagcacga aggaactata
    10741 gaagaaaata attctgaagt tcttgaagca gaaaggcctc aagcagaaga aaccgttcag
    10801 gaaaaatatg accgcagtct taagaaaact cgtacaggtt tcggtgcccg cttgaatgcc
    10861 ttctttgcta acttccgctc tgttgacgaa gaattttttg aggaactgga agaactgctg
    10921 attatgagtg atgttggtgt ccaagtcgct tctaacttaa cggaggaact acgttacgaa
    10981 gccaagcttg aaaatgccaa gaaacctgat gcacttcgtc gtgtcatcat tgagaaattg
    11041 gttgagcttt atgaaaagga tggtagctac gatgaaagca tccacttcca agataacttg
    11101 acagttatgc tctttgttgg tgtgaatggt gttgggaaaa caacttctat cggaaaacta
    11161 gcccaccgct acaaacgaac tggtaagaag gtcatgctgg ttgcagcaga taccttccgt
    11221 gcgggtgcag tagctcagct agctgaatgg ggccgacgag tagatgttcc agtagtaact
    11281 ggacctgaaa aagctgatcc agccagcgtg gtctttgatg gtatggaacg tgccgtggct
    11341 gaaggtatcg atattctcat gattgatact gctggtcgtc tgcaaaataa ggataacctt
    11401 atggctgagt tggaaaagat tggtcgtatt atcaaacgtg ttgtgccaga agcaccacat
    11461 gaaaccttct tggcacttga tgcatcaaca ggtcaaaatg ccctagtaca ggccaaagaa
    11521 ttttcgaaaa tcacaccttt aacgggaatt gttttgacta agattgatgg aactgctcga
    11581 ggaggtgtgg ttctagccat tcgtgaagaa ctcaatattc ctgtaaaatt gattggtttt
    11641 ggtgaaaaaa tcgatgatat tggagagttt aactcagaaa actttatgaa aggtctcttg
    11701 gaaggcttaa tctaatcaga agcaaaaatc ctgcaaggta caaacttgca ggattttttt
    11761 attcgaaacg accatcttga cgataggcga tatctggttg ccaagtccat ttggcatcga
    11821 atttttcaag taggtcaaag ctggcttgag gtcccatgct tccagcttta tagtcatgaa
    11881 gtggggcacc attttcggcc cagagctctt caatacggtc aatcaacttc catgacgcac
    11941 caacctcatc ccagtggcta aagttagttg agttgttatt taggacatca taaatcaatt
    12001 tctcgtatgg ttctggagaa gcaccagttg cagtcgcgtc tgtacgataa tcaagtgagt
    12061 taggagccaa gttaaattct tctcctactt gcttcccatt taggctaaga gagaagcctt
    12121 ctgttggttg aatatagatg gtcaaaatat ttggagcaag tggttctcca aagatagaat
    12181 ccatttgttt aaagacgatg ttgacatgag ttcctttttc agtcagtcgt ttacctgtac
    12241 ggaaaaagaa aggaacacca cgaaatcgat cgctgtctac aaagaaggca ccagatgtaa
    12301 aggtttcagt tgttgattct ggattcacat ttggttcgct acgataagag atgtatttca
    12361 tgccatcaat cttaccagag cggtattgtc cacggataaa gtgttctttg agttcttcat
    12421 cagttggatg atagaggttt ttaaagacct taatcttttc agcacgaatc tcgtcttttg
    12481 tgaagcttgc tggtttgtcc atggcgagga gcgaaagaag ttgtagagtg tggttttgga
    12541 ccatgtcacg gagggcaccg gattggtcat agtagccacc acgttcttct acacccaagc
    12601 gctccgcaaa ggtaatttga acattgtcga taaaatcctt gttccaaacg ttttcaaaaa
    12661 tcaagtttgc aaagcgaact gcaaagatgc tttggatcat ttccttacca agataatggt
    12721 caatacggaa aatttgttct tcgtcaaatg ttgctaggag ttcgtcattc aacttgcttg
    12781 cagttgcgta atctgtacca aatggttttt caacgatcaa gcgctcaaaa cctttgccat
    12841 cgacaatgtt ttcagatttg aggtgtttgg caatggttcc aaagaactga ggtgccatag
    12901 acaagaagaa gagcttattg tgttcagctt ggtatttttc attaagctca gcttgtaatt
    12961 gacgcaaagc aatataatgt tccgaatcat tgacatcatg gctttgatag tagaagtggc
    13021 tagcaaattc ttgggcttgc tcggtactat ctgccaaatc aaggatggac tcgacaacta
    13081 cagattcaaa atattcctta ctccaaggtc tacgggcagt tccaataacg gcaaagtgct
    13141 tggaaagatt gccggattga tatagtctaa aaagggaagg gtagagcttg cgtttagcca
    13201 ggtctccact cgcaccgaaa attgtaacaa taaccttaga tgacatctag ctacctaatt
    13261 tctattttta ttcctagtct tgctaggtat gaggaaacct catttcataa acttaattct
    13321 aacataattt tccgaaaaat caaaaattcc aatacgaggt agaaaaactg caaggaaatc
    13381 cttacagttt gagtttagac gtttaagacc ttatctaaga actctttcag acgagggtgt
    13441 tgtgggttat caaagatttg gtcaggtgtt ccgtcttcaa ggaactcgcc atctgcagta
    13501 aagataacgc ggttggcaac ctgacgagca aatcccatct catgggttac gataatcatg
    13561 gtcatgcctt gctcagccaa ttccttcata acgttaagta cgtctccaac catctcaggg
    13621 tcaagggcag aagttggttc atcgaagagc atgatgtctg gattcattgc taggccacga
    13681 gcgatggcca cacgttgttt ttgaccacct gataggctat ctggattggc attagcttta
    13741 tctgctagtc caaccttttc aagcaactcc attcccaatt cctcagcttc ttccttagtc
    13801 atcaacttgt gctcaatagg agcaaaggtg atgttgtcca atacagacat atgagggaag
    13861 aggttgaagt gttggaatac catgccgata ttttcacgga cgtggtcaac attggttgtt
    13921 ttttcagtta aatcatagcc gttcacagtg atgtgaccgc tagtgacttc ttctaaaaga
    13981 ttgaggctac ggaggaaagt tgacttacca gaacctgaag gaccgatgat acaaacaaca
    14041 tctccttcat agaacttagt cgtaattcct tttaggactt cattttttcc atagtacttg
    14101 tgtaaatcat ttacatcaat ttttaatttt gccattaacg aatcctcttt tctaagcgtt
    14161 tcgctagtct agtcaaaagt gtgataatta caagatagaa gatagcaagg attgcataca
    14221 tcttgaaact ttggtagtta cgagcaatga taatcttacc agtttggaag agttcaacca
    14281 aaccgatagc agatacgata gttgtatctt taagagcgat aacgaattgg ttgacaaagt
    14341 ttggcaacat caatttagtt gattgtggca agataatctt acgcatggtt tttccataag
    14401 agatacccaa gcttcggctg gcttccattt ggccaactgg aacggcctga ataccaccac
    14461 gaacgatttc agcgatataa gcagccgcat tgagtgagag ggcaattgtt ccagctacaa
    14521 agtcgttaat tgggctttgt tggcctgtga tagactcgat gaagtttgga attccccaga
    14581 agatgaaggc tgcaagaatc atcaatggaa taccacgaat aacgtcaacg aaaatctcag
    14641 agatgacgcg aagagatttg tatgggctaa cgctaaacat accgaagata attccgatga
    14701 caatggcaat agcaaatgag ataagagcta gagcaagagt gataccaaga ccgctaagga
    14761 gttgtttgta gttgttttga agcaagcccc agagcgttgt ttcgtcaaca gtacttgttg
    14821 aagcagttga agattcgcta gctaggtatt tgtcaagaat cttttggaat tcaccgtttg
    14881 ctttaaggtt tgcaagtccg ttgttgaaca tttcaatcag ttctggattt gctccttttt
    14941 taacggcaaa ggctgtttca ccgattggag ttccagagat tggagttttc aatttttgac
    15001 cttggctgat agaatatttg agaacaggtt catcatccat aacggcatca atggcaccag
    15061 tgtttaaact gtcatacatt gaagaaccat cagcaaaggt tttgattttg taaccgtatt
    15121 tgctttgatt ttctgttagg aaggtttgag aagcagttcc gtttttaaca ccgactgtct
    15181 ttccttttag atcttcataa gaagcaatat tgcttgattc tttgacacca agaatggtat
    15241 tagcagtgta gtatgattct gagaagtcaa aagttgcctt acgagcatct gtgacagaca
    15301 taccagcaat gataccatcg gcttgaccag cttggacagc actgatagca gcatcaaaac
    15361 cagggttggt gatttcaatt tcaaaacctt ggtctttagc gattgcctta atcaattcca
    15421 tatcaatacc agtgtattgg ttgcttgaat tttggaaaac aaaaggtgca aaagaagaat
    15481 cgctggcaat gatatattta gccttaacag gaatagcttt taatcctgcg agagtagttg
    15541 tagttggcac tgctgaagat gatgaagcag tccatttctt gataatttta tcaagactac
    15601 catctttttt catttcagcc aaggcttggt taaattcagt aaccaggtgc tcgtatttac
    15661 ttcctttttt cacaccgaaa gcaaaacttc ctacagcttc accatccatt tcaatatgga
    15721 ggtcttgacc ttggttaatg gcatattcga taacaggttt gtcatccatc atggcatcga
    15781 tggcaccagc actcaagctg ttgttcatta aatcaccagt gtcaaatgtt ttaatagtaa
    15841 agccgtattt atctttgatt gtttcaagga aacgttgagc ggcagttccg tttttaacac
    15901 caacggtttt gccagttaat tggtcgtact tgctaatttt gtgtgacttt gtagtagcaa
    15961 tgacaacttt tgtatcatag taagtatcag acatggtgaa gactttttca cgttctttag
    16021 tctttgtcat ccctgccatg atagcgtcgg cttgcccagc ttgaaccgca ttgactgctg
    16081 cgtcaaatcc aggataggac atctgaatgt tccagccttt aatctcagcg actttgttaa
    16141 taatgtcaac atcaattcct ttataagttt gatctgaatc tttaaactca aaaggtgcat
    16201 aggcggtatc agaaacaatc ttaatcgttt ctgctttggc aatacctaat gagaaaattg
    16261 ggaataaaat tagcaaaaat gctagaaatt ttttcttcat ttttagtctc cttttc
//
LOCUS       DAVLYV010000039        15843 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLYV010000039 DAVLYV010000000
VERSION     DAVLYV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.039"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..376
                     /locus_tag="VYO70_001244"
                     /pseudo
     CDS             1..376
                     /locus_tag="VYO70_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(493..840)
                     /locus_tag="VYO70_001245"
     CDS             complement(493..840)
                     /locus_tag="VYO70_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1713477.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(951..2726)
                     /gene="spxB"
                     /locus_tag="VYO70_001246"
     CDS             complement(951..2726)
                     /gene="spxB"
                     /locus_tag="VYO70_001246"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1713478.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2933..5176)
                     /locus_tag="VYO70_001247"
     CDS             complement(2933..5176)
                     /locus_tag="VYO70_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1713479.1"
                     /translation="MTEIVKASLENGIQKIRIQAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSICTVPLVILMIGMLAGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(5186..5557)
                     /locus_tag="VYO70_001248"
     CDS             complement(5186..5557)
                     /locus_tag="VYO70_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1713480.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFSFACGMNMMHGKMIVE"
     gene            complement(5568..5963)
                     /locus_tag="VYO70_001249"
     CDS             complement(5568..5963)
                     /locus_tag="VYO70_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1713481.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      6147..6245
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6329..7120
                     /gene="thiD"
                     /locus_tag="VYO70_001250"
     CDS             6329..7120
                     /gene="thiD"
                     /locus_tag="VYO70_001250"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1713482.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7330..7962)
                     /locus_tag="VYO70_001251"
     CDS             complement(7330..7962)
                     /locus_tag="VYO70_001251"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1713483.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7955..8758)
                     /locus_tag="VYO70_001252"
     CDS             complement(7955..8758)
                     /locus_tag="VYO70_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1713484.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8758..9282)
                     /gene="thiW"
                     /locus_tag="VYO70_001253"
     CDS             complement(8758..9282)
                     /gene="thiW"
                     /locus_tag="VYO70_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1713485.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9287..9979)
                     /gene="tenA"
                     /locus_tag="VYO70_001254"
     CDS             complement(9287..9979)
                     /gene="tenA"
                     /locus_tag="VYO70_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1713486.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9990..10640)
                     /locus_tag="VYO70_001255"
     CDS             complement(9990..10640)
                     /locus_tag="VYO70_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1713487.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10642..12027)
                     /locus_tag="VYO70_001256"
     CDS             complement(10642..12027)
                     /locus_tag="VYO70_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713488.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREINQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(12028..12588)
                     /locus_tag="VYO70_001257"
     CDS             complement(12028..12588)
                     /locus_tag="VYO70_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1713489.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12849..12937)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(13146..13775)
                     /locus_tag="VYO70_001258"
     CDS             complement(13146..13775)
                     /locus_tag="VYO70_001258"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1713490.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13777..14559)
                     /gene="thiM"
                     /locus_tag="VYO70_001259"
     CDS             complement(13777..14559)
                     /gene="thiM"
                     /locus_tag="VYO70_001259"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1713491.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVIASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14802..15470)
                     /locus_tag="VYO70_001260"
     CDS             complement(14802..15470)
                     /locus_tag="VYO70_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1713492.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15685..15773)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
BASE COUNT         4782 a         3493 c         2898 g         4670 t
ORIGIN      
        1 atacaggcga atcatttttc ttaatagctg gtggatgtaa tactgagtgg atgaacgtct
       61 ttttagaaga gctttcacaa gcttatccat atgattatct tttactcgtt atggacaatg
      121 ctatatggca taaatcaagt accttaaaga ttccgactaa tattggtttt acctttattc
      181 ctccatacac accagagatg aacccattga acaagtgtgg aaagagattc gtaaacgtgg
      241 atttaagaat aaggcctttc gaactttgga agatgtcatg aatcaacttc aagatgtcat
      301 acaaggactg gagaaggagg tgataaagtc catcgttaat cggagatgga ctagaatgct
      361 ttttgaaagc atatgagtat tatatgcaat ttctttatat aaaaagaccg gattgctccg
      421 atctttcaat agttcatatt ctcaatttct attttaaaaa tagctaaggt taacgtcaaa
      481 tgactacgcg acctatttca tacgataaaa atcaagcact agaccagcag gtccttgaac
      541 taataaggac tctgttcccc aatcggttac agttggtccg tgtaaaacct ttataccaag
      601 ctcgttcaac cgtttgtagt tctggtctac atcctcaacc tcgatatgaa taatgattcc
      661 tgactgaaag ttttccaaag gaaccaaatg attttgtgac aacataaggc agtgactacc
      721 aatcgtaaac tgagcaaaac catcatcagc ataatctgcc tttttatcca aaatatgctc
      781 caagtcagca cagacttggg gaacatttga aacgataata tctaattgat ttaaattcat
      841 ttactctcct ccataaaaag accggattgc tccgatcttt taaagttctg ctctatgaaa
      901 atcaaagaat aaagtctaca agtttcatat ttgattttcg gcgagaggaa ttatttaatt
      961 gcgcgtgatt gcaatccttc ttcttccaag aagagacgga atggtacgag ttcttctgct
     1021 tcgtattttt ccttgaaggc tttgatagct tcttctgagt gaagttttgg atccaattca
     1081 agtacttcta ctggaagtgg acggtgttga gtgatgcgag catcgatgac aacagtttta
     1141 ccttctttgt tcaatttaac agcttctgca acaactgcat cgatgtcttc gatacggtca
     1201 actgtgaatc caacagctcc ttgagcttcc gcaattttag cgtagtcagc gtttgtgaag
     1261 tctacaccaa acaagtgttt gtttgtatct tcgtatttgt tcttgatgaa gccgtactca
     1321 gcatttgaga agacaaggtt gataactgga aggtcgtatt gaacgtttgt gataacgtct
     1381 gggtagcaca tgttgaatgc tccgtcaccc atgatgttcc atacttggcg atctggattg
     1441 tctttcttag cagcgatacc accaggaagg gcaataccca ttgtcgcaaa gagtggagat
     1501 gtacgccaca tgttcttagg tgtcatgtga aggtgacgag tagatgtttg agtagtgtta
     1561 cctacgtcga ttgagtagat agcgtcttga tcagcatgtt tgttgattgc attgtaaact
     1621 tgatacaatt gcaattcacc ctcagtttta ccttcgagtt tgttcatgta atcacgccag
     1681 ttttggttgt tcttaacgtt tgcacgccac catggagttg attcaactgg gtttactttg
     1741 tcaaggatag ctttagctgc ttgaccagca tcaccaagga ttgaagcgtc aagggcatga
     1801 cgtttaccaa gtttgtaagg gtcgatatca acttggatga atttttcagt gttcttgaat
     1861 gcttcgtaaa cttcagcaaa tgggaagttt gaaccaagga aaagaactgt gtctgcttca
     1921 aagaccactt cgttggctgg tttccaacca acacgataag cagaacctgt caaaccttca
     1981 tagttccatt cgaaagcttc aaagttttta ccagttgtga tgattggtgc tttgatttta
     2041 cgtgacaatt cagtaatcac ttcaccagct ttaacaccac caaatccagc atagataact
     2101 gggcgttcag cattgttcaa gatttcaaca gctttgtcga tttcaacttc gttcaaagca
     2161 ggagcgatga atgagcgttc gtatgaacct gaaccgtagt atgagttttc gtcgatttct
     2221 tggaaaccga agtttactgg aatttcaaca acagctggac cttttttaga aactgcagca
     2281 cggcaggctt cgtcaattac ttttggcaat tgctcagcgt aagctacacg tttgttgtaa
     2341 acagcgatac cgttgtacat tgggttttgg ttaagctctt ggaaagcatc catgttcaat
     2401 tcgttaactg gacgtgatcc aaggattgct aggaatggag tgttatccat agctgcatcg
     2461 taaacaccgt taatcaagtg agtcgcacct ggaccacctg aaccaactgc aaccccgatt
     2521 gagccgccga atttagcttg cataaccgct gcaagagcac ctgtctcttc gtggcgaact
     2581 tgtaagaagc ggatatcttt gtcttcagcc aaagcgtcca tcaatgagct gagtgttcct
     2641 gatgggatac cgtagattgt atctacgccc catgttttca atacgttaag cattgctgca
     2701 gatgcagtaa ttttcccttg agtcataatg ataactctcc ttcaattttt ttagacttgg
     2761 agaatacgat tacatagaat tggaaacgtt ctccaaattt ttactattcc actgtatcat
     2821 atttatgctg acttttctaa aaatctgctc aaaactctct attctctatt ctaatacagt
     2881 tttgaaagtg tcgtcatttc tgttttataa caaaaaaatc tagtcattac ttttagtcta
     2941 ttttactaaa atttaacaga agggaactgg tcagaacaga tacagaacta aaggccatgg
     3001 ctagacctgc caattctggg ttgagagcca gtccaacacc tgaaaagact cctgctgcaa
     3061 tcggaattcc gacaacattg tagataaaag cccagaaaag attgagcaga atacgatgaa
     3121 aggttttctt actcatgtcg aaggcacgca ccactcctaa aagattattg gttgtcaaca
     3181 ccaaatctgc tgactcgatg gcgatatctg ttccagctcc catagcaatc cccacatctg
     3241 ctacactaag ggcaggagcg tcattgatcc catcaccaac aaaggctact ttacctgact
     3301 gttgcagttt atggatttca tgggcttttt cttctggcaa gacgcctgca atgacctctt
     3361 caattccgat ttgatctgca atagcacgcg ccacaccagc attgtctcct gtcagcatga
     3421 ctgtcccgag accacgtttt tttagctgac tgatggctag cttagcattt ttcttaggaa
     3481 tatcttgcaa agcaagcaag cctttgattt cattgtcaac agctaagaac acaactgtct
     3541 tagcttcttt ttctagttct tctagtttat cttgataagt attagaaata tccatgccat
     3601 ccagcatttt agcatttcca agtaaaactt gttttccatt gattcgccct gaaacacctt
     3661 tcccgtgcaa ggcctgaaaa ttttcaacag tttgaaactc aagtccagct tcactcgctc
     3721 gcttaacgat agcctcagcc agtgggtgtt gagaagcatc ttccaaggag gctgccaatc
     3781 caaacacttc tacttcgtcg ccgatgacat ctgttaccac aggtttccct tccgtcaaag
     3841 tcccggtctt atcaaagaca agggtttgaa ctttctggat ttcctgtaag acagttccat
     3901 ttttgaggag aacccccatc ttggcactac gtcctgtccc caccataagg gctgtcggtg
     3961 ttgcaagtcc caaggcacaa ggacaggcga taatcaaaac cgccactccg tagagaagag
     4021 aggacacaaa gctagctcca agcacaacca cactatccct gagcaagacg aaccaaaccc
     4081 aaaaggtcat gattcctaaa atgacaacta ctgggacaaa aatccctgaa atcttatccg
     4141 tcaagtcctg aatcggcgca cgacttgtct gagctttctt cacaaaatcc acaatctgag
     4201 ccaaaacagt ctctgagcca actttttctg ctctaaagac aagcgttcca ctatgattga
     4261 tggttgagcc aatgacagta tctccaactg tcttgtccac aggcagactc tcacctgtca
     4321 ccatggattc gtcaatacta gagacacctt ctactacgac accatcaaca gcaatctttt
     4381 caccgggacg cactcgaatc aggtcgccta ccttgacttg ttccaaagga acttggacat
     4441 aactatcatc actcaagact tctgcggttt tagcttgcaa gtccagtaat ttctccacag
     4501 cttgggacgt attttttctc attttttcct caaaaactgc tcccaaaaga acgaaaaaga
     4561 ggataaatcc agcactttcg aagtaaacag ggagaccagc aaagagagca actaggctat
     4621 agaaataagc cactagagtt cccagcgcaa ccaaggtatc catgttggca ttgtgctttt
     4681 taaaactggc ccaagcactc tggatatatg gcttacctgc aactaacata ataggcgttg
     4741 ttgctagaaa ggttccccaa tgcatgactt gatgactaat gctacctgcc aacatcccaa
     4801 tcatgagaat cacaagaggc acagtacaga tactagtaat ccaaaaacgt tgcaggagag
     4861 atagagattt tcgagtcttc tcaacgactg tatagcttcc cttttgcatc ttcatgccac
     4921 aagaaaattc atgtcgccct aattcttgag gtgtaaaacg aatgactttc tcctcatcta
     4981 cgccgattgg ttccaagata ccttcttctt caaacagaat ttccttataa cagtttgaag
     5041 gagtagcacg atgaaaggta atctcagctg gaattccctt ttgaagctgg atatgggctg
     5101 gatgatagcc tttttcagct tggatacgga ttttttgaat gccattttct aagcttgctt
     5161 tcacaatttc tgtcatagtc tccacctact ctacaatcat cttgccgtgc atcatgttca
     5221 taccacaagc aaagctaaac tctccagcct gttcaggcgt gatttccact acatactctt
     5281 cccccattgg caggttcgca tgtacaccaa aatctggaaa aacaatttga tccagacatg
     5341 gtgaaggatc cttgcggtca aagacaatgc gggctggcac tgatttcttg aggacaatca
     5401 actcaggagt atagcctccc atgacttcca ctcgaatctc ttggtatccg tttttttgct
     5461 gggctttttg tccagatttt tcaggctttt tgaaaaacca aaacaagata aacgcgataa
     5521 gggcaataca aataatggtt acaatactat ttaacatgac gtctccttta catacaatta
     5581 catcttactt ctgttacagc acttgatttc ttctctgaaa tcacagcttc caagtcttcc
     5641 aagtcagtct gagtaaattc acattctaca atcaagtcag ccaacaaatt cctaatccta
     5701 cgggaacaaa ccttgtcttt gatatcttgg acaagtaaat cccgactttg gtctagagtt
     5761 aaaagcgctg aataaacaaa ggacttgcct tcttttttcc gagtcaaaca ctctttctca
     5821 actagacgag ccaaaagtgt ctgaaccgtg gacttggacc agtcaaaccg ctctgccaaa
     5881 accctaatca aatctgtact ggtctgctcc ccctgcatcc aaataatctt catgacctgc
     5941 cattctgcat ctgaaatctg cattaccata cctccaaaat ctacatttgt caattacact
     6001 caacagtata ctcccaaaat ctacatttgt caattataga aataatattt tcttcgaaaa
     6061 atagaatttt aatcatttga aaaacgattt gcagtcaaat attactatat aaacaataaa
     6121 aatatgctat actaaagaaa aaagaaaaca accactaggg gtgcgtaaag ctgagattaa
     6181 cgactgttag atccctctga ctcaatctag gtaatgctag ctgatggaag tggaaatgat
     6241 aatggggact agcagtcttc tattgccttt ctaaaacaga ctagcttgtt cttaagaata
     6301 caaacttcag ttggttggga ggttttagat gacttattta cccgttgctt tgaccattgc
     6361 agggactgac cctagtggtg gtgctggcat tatggcagat ttaaagtcat tccaagcgag
     6421 agatgtctat ggaatggctg ttgtaaccag tcttgtcgct caaaatacca gaggtgttca
     6481 gctaatcgag cacgtttctc ctcaaatgtt gaaagcccaa ttggagagtg tcttttctga
     6541 tattccacct caggctgtaa aaactggaat gttggctact actgaaatca tggaaatcat
     6601 ccaaccctat cttaaaaaac tggattgtcc ctatgtcctt gatcctgtta tggttgctac
     6661 aagtggagat gccttgattg actcaagtgc tagagactat ctcaaaacaa acttactacc
     6721 tctagcaact attattacgc caaatcttcc tgaagcagaa gagattgttg gtttttcaat
     6781 ccatgacccc gaagacatgc agcgtgctgg tcgcctgatt ttaaaagaat ttggtcctca
     6841 gtctgtggtt atcaaaggcg gacatctcaa aggtggtgct aaagatttcc tctttaccaa
     6901 gaatgaacaa tttgtctggg aaagcccacg aattcaaacc tgtcacaccc atggtactgg
     6961 atgtaccttt gctgcagtga ttactgctga actagccaag ggcaagagtc tttaccaggc
     7021 agttgataag gccaaggcct ttatcacaaa agctattcaa gatgcccctc aactcggtca
     7081 tggttctggt ccagtcaacc atacaacttt taaagattaa gaaaaaaaac tctctagttc
     7141 ccactttaag ggaattagag agtttttata ctcttcgaaa atctcttcaa actacgtcag
     7201 cttccatctg caacttcaaa acactgtttt gagctgactt cgtcagtctt atctaaaacc
     7261 tcaaggcagt actttgagca acctgcgact agctttctag tttactcttt gattttcatt
     7321 gagtattaat taggaaagaa tgtcatgcaa ctttgttaaa aaggcttgcg tttttgcctc
     7381 aatatcttct gcttgcatca aatcacgtac aacagctaca ccagctatgc cagtgcccat
     7441 aagctgatca atattctccg aagtcaagcc accaatagca actattggaa tggcaaccgt
     7501 ttggcaaatt gttttcaagg ttgaaatcag ggtgatgggt gcattttcct tggtggttgt
     7561 cggaaaaatg gctcctgtcc ccaagtaatc tgcacctgat ttctccgctt ccagagctct
     7621 tttaaccgtt ttagcggtga caccgaggat tttttcagga cccaagactt gtcgggcaac
     7681 cgaaactggg agttcgtcat ctccaatatg cagaccagca gcatcaaccg caagacaaac
     7741 atccaaccga tcatcgatta tcaagggtac ctgataagca tctgttattt ccttgacttg
     7801 ttttgccagt tgataatatt gattggttgt gagatttttt tctcgcaatt ggactatggt
     7861 aacccctgaa cggcaggccg tctcaacttt tgcaagaaag ctttccacgg aatcttgata
     7921 gcgattggtt accagatata gtctaagtgc ttctctattc ataaacctct cctttgatgg
     7981 tatctagcca attttcatct cttcttagga gcgaaagctg attgagtact tggtaacgaa
     8041 attcttccaa tcccattcct tgaacaacta ttttctcagc agcgatattg agataagaga
     8101 ctgctaagca agaagcttca aaaccagtct ttccttggct gagaaaaacg gctgttaagg
     8161 ctccaaccaa atctcctgtc cctgttatcc agtctaattc tgcacagcca tttcccagta
     8221 cagcgacctg atttttcgaa acgacgaggt ccttgggacc tgtgactaag aaagacatac
     8281 catgataggt ctgacaccag tctttcaaga cttgaagcaa atcctccgtt tcttgatctt
     8341 tagcactcgc atcgacccca acgccgtggt gctttaatcc aacaagactt cgaatttctg
     8401 acatgtttcc tttaaggacc gtaggtctat agtctaaaag gtctctgact aagctcttac
     8461 gaatggatga agctgttacg ccaaccgcat ctactaccat tgggagacaa acttgagctg
     8521 cataagaagc tgctatacgg attgctttct ccttctcagc tgacaaatgc cccaaattga
     8581 tgaagagagc ctgactttgc ttagtaaaat caagaacctc acggggatca tctgccatga
     8641 caggtttgca tcccagagcc aaaatcccat ttgccagcat ctcacaagaa atctcattgg
     8701 tcatacagtg aatgagggaa ctagagccta taggaaaggg atttgtaaat tcctgcatca
     8761 gtctatcctt tcactaaaga aatatccctg cactttttta aagaattcct gcttgattaa
     8821 aaatcgaaag gcaataaagg aaatcgctgt accaatcaag gttgctccga aaaatcgagg
     8881 cgtgtagata aaccagctaa gcttagcagc tgatcctgta aagagtacca taacaggata
     8941 ggaaacaata gaaccaataa tacctgttcc cacaatttct cccaaggcag aaaagtaaaa
     9001 ttttcgaccg tacttataaa agagacctgc tagaagggct ccaaaagtcg ctcctgtgag
     9061 agctaaaggc ggaatccctt gagtcgtcat acggataaag gctgtcactg tagccatagc
     9121 caaggcataa acaggtccca tcatgattcc cgctagaata ttgactacac tggacatcgg
     9181 tgccattccc tcaatccgaa agataggtgt aaggactaca tcaagggcaa tcatcataga
     9241 taaaatggtc aatttgtgaa cttgtagttg gtgctttctc atgtttctat tcttctcctt
     9301 tttctaaaga ctgtaaatcg ctcttccatg tctggtgttg gtaagccatc tcccaaaact
     9361 tggcttccat atgaacactg atgtggaagg catctagcat tttttgctta tctgtctcat
     9421 cactttctcg atagagctga ttgaccagtg ctccctcctc tctgatctgt tgctctaact
     9481 catccgtaat ataagtttca atccattgtt gatagagagg atttggtgat ggtttaagat
     9541 taagtgattt gcctatatca tggtataacc aaggacaagg aagcaagctt gcaaaagcga
     9601 tggctaagtt cggttctgca aattgcctat aaatatgaga aatgtaatga taacaggttg
     9661 gagcgattgg atgttgctcc atttcctggt cgctgatttc caattccttg aaaaattgtt
     9721 ggcgaataaa taactcaccc tccactaaac cctgagcatt ttgtttcaag agtcttttca
     9781 tctcttggtt tgaagtctta tcagccaaaa gatgatagat ttctgagaaa gccttcagat
     9841 agtaggcatc ctgaatcagg taatagcgga aaatggcagg ttctaaattc ccctcttgta
     9901 attgtaaaat aaagggatga tgaaaggaag cctgccaagc tttcttggat aattccatcg
     9961 caatatctgt aaattccata ataactcctt tataaaaata gactggtttg aagcaataaa
    10021 aagaaaagca ggtagattaa ttttgttttt ttaggaatat aaaaagtccg atagctattc
    10081 ttcaactgtg catgttcgtc atatccatga gcagacagag ctctcaggta aagatggcgc
    10141 cacctaaaga ctgtcatcag aaccttactg taaatcaagg gcgaccaaaa atgcagttct
    10201 tgaccacgta ataggcaagc ttctttgagg gacttgattt cttgctgaat gagaggaaag
    10261 gaattgaata ccacaatcaa ggcataggac caagagcgtg atagcccctt ttgagccaag
    10321 tacaagagaa gctcttttag tgaaatagag gaaacaaaga caaggccgat acaaactgtc
    10381 acaaaggccc tcgttccaag catgactgcc tgtgaagcat ctccgtgtaa ctgaactgcc
    10441 cagtagttgg caaaagatgg taaaatggca agtatgatca tccaagctaa cattttaaat
    10501 cgacggtaat agagcataaa gagaatacaa aatgcgacta ccgaaagatt cagagcaatc
    10561 gaaggaatga aagatgtttc caaggataaa atcagcaaga agagactgat aatcggtgtc
    10621 tgggttgcta ctttgaccat actatctcac ctccccttgg gtattgctac tctgagatgt
    10681 aagtggtttg gtaatggtca cttctttcac atgccgaaga ccctgactag tcatctcaat
    10741 ccaataatca accacagaaa tcaaagggtc taaacgatga ctaatgagca gaaaacttct
    10801 tccttgattc ctctcctcca caatccactt gcaaaaataa tggcaggctc tatcatccaa
    10861 acctgcaaaa ggttcatcta gcaagatcac ggaagcctta ctggtcaaga tggtcaggag
    10921 ctgaagaatt ttttgctgac caccacttaa ttgataggga ctcttatcga ctgcctgctc
    10981 caaatcaaaa tatcgtaaag cttgaaaaat ccgctgattt ctttcagaat caggtccatc
    11041 taattgaagc tcctctcgca gactgactcg gataaactgc ttctcagctt cctgaacaac
    11101 acccgtcaga tcacggtaca aactcttttt ctttttcagg actgtcccct tccatgcaat
    11161 gcgcccctta tacttttgaa attgaagaat agaccgaaag agggttgatt tcccgacacc
    11221 attgtcaccc aggatacagg aaatcccttg atagaatgtg aaatcagcaa ttgaaaagag
    11281 ggggcgatta ccaagctcac cagtcacacg gttcatatgg aatagttccg ggctagaagc
    11341 aacttccttt gaagcaacct gtgtcatctc ataggaaggg atttgattta tttcccttag
    11401 ttttccgtct cttagctcaa ccatatggtc gatataggct ttatagtcag ataaatcatg
    11461 gtcgcacaaa ataactgtct tcccatcaag agccaactct tttagaatct ccaatatctc
    11521 gattctgctc ttgcggtcaa tggaagcaaa aggttcatcc aagagataga ccctaggatt
    11581 catggcaaag aggacagcca gcgctgcttt ttgcttttcc ccacctgata ggtgatgaat
    11641 gggacggtgt aagattgcct cgcaacgaca ttgctggaca acctctgcta ttttagaatc
    11701 aatttcctga aggtgatagc cgatattctc caaggtgaaa atcagctcct caaacaagct
    11761 ctccatggta aattgatgat tgggattttg aaagagaatg ccaaccttct ggacacgtgc
    11821 gatgatagaa agttgactga cctcgctccc atctatcagg acttgaccac tatagggaag
    11881 agaactgact tgagcaatga cttgaaaaag gctggatttt cctgaaccac tgctcccaac
    11941 taacaaggta aaggcttgcg catgaaaagt aaaatcaaac ggctcagaga agattgggga
    12001 ctgaatcgct cgtagttcca gacccatcta tgcttttcct ccagttgcaa actgatgata
    12061 gagtttgaca atggcgcgaa ccaagatggt acagaagaaa taaacagaaa taaaacgtac
    12121 cacaagtaag gaaaggacaa acggaaggga aaaggcgtag taacctaact taatgtattc
    12181 atagacaaag ctaacaagcg taatcccaat actattagca gttagagaga gccaactttc
    12241 atagcgattc ttagttacga taaaaccaaa ttcacttccc aaaccttgaa ccaagccaga
    12301 caaaagagct cctagaccaa attggctacc ataaaggact tcagcaagcg cagctagcac
    12361 ttctccaatc gttgcacttc cgactctcgg aacaaagatg gcagcaatgg gcgcagccat
    12421 acaccagaga ccgaagagga tttcattggc aaaggcctgc aaaccaagag gtgttaagag
    12481 tagactgaga atattataca catatcctga accaacgaaa accccaccaa aaaagataga
    12541 caagaaagca agcaagataa catcttttaa ctgccatttt ttcaacataa aaaactcctt
    12601 ttttaaagaa aagtggggca ctcaagaaga ccgacctaaa tactttgtat agcagactga
    12661 atttagaaca gtacacaaga atgctaaaat atttctagaa attaatttga attttctaat
    12721 tgatttgttc gcatcttatt tcaatctact atatcatctt catccagttt cgtaaaagaa
    12781 aaaactctaa ttacagatac gaattagagt tcagcttaca agattagaca gttcttttcg
    12841 acatacgaaa aaaacatttc acatttccct tcgccagtct taactgtatc aggttcaatg
    12901 ggtatcatct cagcctaaag caccccaaat gcctttatta tttaattatg tgattattat
    12961 aacacacatt ttatactagt tcaagaaatt gaactggaaa tacagccttg cactcacaaa
    13021 gacagcagat ctttcttttg caaaaaacaa atgacctgtt tgatgaatta gctattcaag
    13081 ctgaatctag acatagcttt ttaaaaaagg aaaatcctac ttacttagaa tccaaggata
    13141 gatatctatt gttcactcat ttcccgaaca gttttttcta tattttttgc atacgatatt
    13201 gccgaaatga ttgaaacgcc gtcaacattg gtctttataa tgtctttaat atgtttcgtc
    13261 tgtatcccac caattgcaac taaaggcatt tgtggcaata gttttctcat caattcaaga
    13321 ccttcataac ctatagcacc accagcatca tcctttgact gggtatcaaa tacaggacca
    13381 acaccaacat aatctacata ttcaactttt gattgttgaa attcttcctc gttttttata
    13441 gaaagaccaa ttattttatc tggcatcaat tttctaattt catcaacacc aatatcatct
    13501 tgacctacat gtacgccatc agcgtcaatt tccattgcca aatcgatatc atcattaacg
    13561 ataaacggca cattgtattt tttacaaagt tctttaattt ggatagctag ttcaagtttt
    13621 tctaagcctt ctaaagcacc ctcacctttt tctcgaaatt gaaataaggt tataccacct
    13681 tttaaggctt cctcaacgac tgtatataga ttttttcctt ggcaagtagt cgttccacaa
    13741 ataaaatata gttttagtaa ttctttatga aacatcttac ttcactcttt tgaattcctt
    13801 tacatcttca tctgtaatct cgtataaggc atttataaat tcaactttaa atgtcccagg
    13861 aagatgtcca tttggacgtt tttctgccat ttctccagcg atattgtaaa ccaacattgc
    13921 tgtttctaat gatttcaatt cttgaccttt ttctagtccg ataaagcttg ctatcactgc
    13981 ccctaataag catcctgtcc caatgacttt cggcatcata gcactaccat tatgaatcgt
    14041 taccacttct ccattaacag caatggcatc cacttcacct gttactacta ttggaatatt
    14101 gaacttctca tttgctgcta gagcaatttc gtcaatatta tctacgcccg cactatctac
    14161 tcctttagat gccacatcta ttcctactaa agaggcaatc tcgccagcat ttcctctaat
    14221 cgctgctagt ttataattgt tgattagatc atctgctact ttttttctat attctcctgc
    14281 tccacaggct acaggatcta aaactgctgg gacattatat ttctctgcaa ttttcagagc
    14341 agcttggtat aatttccaat tttcatctgt caatgttcct atgtttatta ataaaccacc
    14401 agcatacttt aacaaatcct ctaaatctgc tggaaactca ctcatggctg gtgaggcgcc
    14461 cagtgctact aatccatttg ctgtgaaatt ttttactaca tcattggtta tacaaatgac
    14521 caatggtgct ttttctttta ataattttaa acttgtcata ttgaaatcct tccttttcac
    14581 tttatacgat ctactgattt cgatttattt ctatctttcg ttaagaattt ttttcattta
    14641 cattgaatga tttatactca atgaaaatca aagagcaaac taggaggcta accgcaggtt
    14701 gctcaaaaca ctgttttgag gttgtggata gaactgacgt ggtttgaaga gattttcgaa
    14761 gagtcttacc tcatcaaatt tgtaaatatc atgagccttc tctagacatc gtaaccaata
    14821 tcaaaaaaag ctaattctaa agcgactgct tgattccagc gttgctgaag ttctgtcaaa
    14881 tcttctcgat ttttaccgac acgattgagt tcgtcaacca gaaattgaac ccactctgca
    14941 aagaaaggac ctctgtggag attgatccat tccgaatgaa tatagacttc aggtaaagcc
    15001 aaatctttag aaccccagtc taaatagaga ccttctgcaa tgaccagcat gaccaaaaga
    15061 tgggcatagt ctgatgaagc caccgccgaa tacattagat cctgaaaggc ttttgttaca
    15121 gggtgcaaag tcacttctag atagtcattc tctgctactt ttaactcttt aaaagccttt
    15181 tggaaataac catcttcatc tgcttcaaga aagcctagtt gcttggcaaa acgaagcttg
    15241 gattcaagtt tatctgcgtg agctacgcag gcacccagca tggataagaa ggcatcaaag
    15301 aagtgataat cttgaatcag atagtccttt aagaccttat tctcaattgt ccccgcaaaa
    15361 agttccttaa caaaacgatg attgattgca gcctgccaat ccttctgact gctttttaat
    15421 aattctccaa cagtcaaacc tggctgaaat gcatagtctt gtgtttccat atttacttct
    15481 cctctcttta cttgttagta attaataaaa caccaagaaa tatcaagcaa aatcgtaatt
    15541 ccacttgatc cttttaaagc acatcgagag catttgcaga gagctaacta aacaagccta
    15601 tccagtttat ataaacaaaa aactccaatt acaatcaaga attagagttg acttacaaga
    15661 ttagatcgtt catttcgcca tacgaaaaaa cgtttcacat ttcccttcgc cagtcttaac
    15721 tgtatcaggt tcaatgggta tcatctcagc ttaaagcacc ccaaatgtct ctattattta
    15781 attactgaac cagtatagca aaaaatgaaa gccctagcaa gatatttgac cgaaaaatat
    15841 ctt
//
LOCUS       DAVLYV010000040        15256 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLYV010000040 DAVLYV010000000
VERSION     DAVLYV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15256
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.040"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..285)
                     /locus_tag="VYO70_001261"
     CDS             complement(<1..285)
                     /locus_tag="VYO70_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1713493.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFY"
     gene            complement(605..1504)
                     /locus_tag="VYO70_001262"
     CDS             complement(605..1504)
                     /locus_tag="VYO70_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713494.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDEFLVKKLTEEWQEDVKKYL"
     gene            complement(1576..2136)
                     /gene="rnmV"
                     /locus_tag="VYO70_001263"
     CDS             complement(1576..2136)
                     /gene="rnmV"
                     /locus_tag="VYO70_001263"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1713495.1"
                     /translation="MKERISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(2136..2909)
                     /locus_tag="VYO70_001264"
     CDS             complement(2136..2909)
                     /locus_tag="VYO70_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1713496.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALKLVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(3214..4107)
                     /locus_tag="VYO70_001265"
                     /pseudo
     CDS             complement(3214..4107)
                     /locus_tag="VYO70_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            complement(4391..4525)
                     /gene="rpmH"
                     /locus_tag="VYO70_001266"
     CDS             complement(4391..4525)
                     /gene="rpmH"
                     /locus_tag="VYO70_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1713497.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(4670..5884)
                     /locus_tag="VYO70_001267"
     CDS             complement(4670..5884)
                     /locus_tag="VYO70_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1713498.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(6040..6334)
                     /locus_tag="VYO70_001268"
                     /pseudo
     CDS             complement(6040..6334)
                     /locus_tag="VYO70_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6496..6948
                     /locus_tag="VYO70_001269"
     CDS             6496..6948
                     /locus_tag="VYO70_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1713499.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(6983..8371)
                     /locus_tag="VYO70_001270"
     CDS             complement(6983..8371)
                     /locus_tag="VYO70_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1713500.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            8437..9399
                     /locus_tag="VYO70_001271"
     CDS             8437..9399
                     /locus_tag="VYO70_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1713501.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPYIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(9428..9811)
                     /locus_tag="VYO70_001272"
     CDS             complement(9428..9811)
                     /locus_tag="VYO70_001272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713502.1"
                     /translation="MNTIERTRRLIKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDETNEVSSKHVFEVVDRTDEVSSKHVFEVVDRTDE
                     VSSKHVFEVVDETDEVSNHTYGKAT"
     gene            complement(9875..10885)
                     /gene="ccpA"
                     /locus_tag="VYO70_001273"
     CDS             complement(9875..10885)
                     /gene="ccpA"
                     /locus_tag="VYO70_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1713503.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(11310..11909)
                     /locus_tag="VYO70_001274"
     CDS             complement(11310..11909)
                     /locus_tag="VYO70_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1713504.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLMLQPDVESVFQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERNIADLMQTLHTVLEGRKEYSPELMEVVMMHPNPLTEQEIAVLKGIAQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(11910..13007)
                     /locus_tag="VYO70_001275"
     CDS             complement(11910..13007)
                     /locus_tag="VYO70_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1713505.1"
                     /translation="MLERLKRIHYMFWISLIFMIFPILSVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLYWGLMLTYVVGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGQLLIFQRIEVEFLFYLLVILTFVDLMTFGLVRIRIVEDLKEAQ
                     VKQNAQINLLLAENERSRIGQDLHDSLGHTFAMLSVKTDLALQLFQMEAYPQVEKELK
                     EIHQISKDSMNEVRTIVENLKSRTLTSELETVKKMLEIAGIEVQVENHLDKSSLTQEL
                     ESTASMILLELVTNIIKHAKASKVYLKLERTEKELILTVRDDGCGFASISGDELHTVR
                     NRVFPFSGEVSVISQKHPTEVQVRLLYKERK"
     gene            complement(13007..13744)
                     /locus_tag="VYO70_001276"
     CDS             complement(13007..13744)
                     /locus_tag="VYO70_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713506.1"
                     /translation="MKRWLALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTGFSMMSTAIFSFPAVLHTDKMNNWQKILRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLILGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPVSLFPDWMQAIGKRLPSYQLMELIKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(13746..14630)
                     /locus_tag="VYO70_001277"
     CDS             complement(13746..14630)
                     /locus_tag="VYO70_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713507.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEVDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     GIEKHFILPLAYKEVIEQSNLVENWSQKQDALQVVTREADAFWELLVQAGCGIQEIEV
                     NNRSLLDTIFEETQKGDN"
     gene            complement(14617..14811)
                     /locus_tag="VYO70_001278"
     CDS             complement(14617..14811)
                     /locus_tag="VYO70_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713508.1"
                     /translation="MKKGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFPMGMEWLKGESHDCG"
     gene            complement(14808..15014)
                     /locus_tag="VYO70_001279"
                     /pseudo
     CDS             complement(14808..15014)
                     /locus_tag="VYO70_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4493 a         3504 c         2673 g         4586 t
ORIGIN      
        1 gtagaatgca actttctctc cccaagaagt acgaatccat gcacgagaag tccctccagc
       61 accagcatat cccttgtcag atgaaccagt ttttctgcta acaactgttg aactatggta
      121 ttttgaaggg tgatagtagt ttgagtaagc accaccgtta cctgaaccat aagtccaaac
      181 accaccttct gggtattgaa cggctgcaga ggcaactgat acacctccac ctaatgttgc
      241 tgcaatgctc gctacaagag ctaacttttt aactgtttgt ttcatattga tactcctcta
      301 tttttgatta caagattgtt gagttacttg ctcctctttc cattatcaac gttgatacta
      361 ttatattacc aaatgatatt ctaaaattat ttgtttttct ctaaattgtc aaaatagcac
      421 cctgaaatga acttttatag tcattatact ccatctttat cacactatca aggacatcaa
      481 tgtccttaaa atctaactat ttcatattat ttttagctta tatctaaaaa aacagttcta
      541 aagacgaact ttagaactgt tttgatacga ggacaaggac atttttgtca ctgattcact
      601 atgtttataa atattttttg acatcctctt gccattcctc tgtcaatttc tttactagaa
      661 attcatctcc aattagcttc gcaaatgttt tagaagactg ataaaatgat tcagctaaac
      721 taaaatctct ttctactctt aatgcttgct tccacaaaaa cataaataca agaggtttct
      781 tttgataatc ttgagtttta tccataattt cttgtaaaca cgatagataa tcattaaaac
      841 aatcagttac tcctaccttt tcaagacaac acattcctga aataagagta tctctaatta
      901 taaagtaatt ctctatttga gttaccttct tctgttccaa aagaaagagc attagctttt
      961 cgatttgatt tataaatttg gcaagatctt ttctaatgag catttgataa aaaaacaaac
     1021 gaatcaacaa catatcattt attgaataag cttctttttc tataatctga ggcatatgtt
     1081 cctcaagtat taactctgca tattcaggcc ttctagttct agaagtatcc aaacacgcct
     1141 gcaatacttc acatgctaat tgttcttctt taggaagatt atcaaagtat gttttataaa
     1201 tctcttccaa acacgcttcc ttcttatcgt actcctcttc tttaccatag attggttcac
     1261 gtaaaatctg gtatttcaat tctaaataag cagaaggaag agctgaaaaa tccggcataa
     1321 ggctataaac tggaactcct agccggcgag caatataggc taacttgggc aaactcggtt
     1381 gcgaagctcc actttcaatt cgcgacagtt gacgaactgt cagttcttgc tcatctccac
     1441 aaaaatctgg acgactaatt cctttttcta tccgaatctc tttaattttt tgaccaatta
     1501 acatcagacg cctcctcttc tacctgcttc gaaaaatact gtgaaactta aaaaattgta
     1561 acggctacat ctttcctact cataagattt catagcttct tccacttctg ccaaagtaac
     1621 cccaaacaac tctaggcgtt tgaggagttg cttgccgtta gaatagccga ttcggagagt
     1681 ctctccgaga tattctctac gcttacggct gtctgcccct gctagaaaac caaggcgaat
     1741 caagtcgcta cgactaatgt caaactgact ctcatgttca aattgttctg tcacctgagc
     1801 tagagccgtt ttcaggtctt catagctggc atgctcaatt cccagagaac gccccttggt
     1861 cttggacttg ggaacagctt catctcgctt gagaaaggca tgctgaactg ttggaatggc
     1921 cgtcataatc atgcgccgaa tccgctcccc attaaaatct gggtctgtaa agacaatgac
     1981 tccatgacgt tggtgtagac gctgaatccg ctctatgtcc tggtcattga tggcagaacc
     2041 tcgagtctca taggtctcca catcgaaata acgcttgaga ttgaccgtat catcacgacc
     2101 ttcaaccacg ataacttggg aaattctctc tttcattact tgctgtccaa tccaaaaatt
     2161 cgttctgcat ttgcagtcgt tgctaccgcc agctcttctg tcgtcatacc acgcaagtca
     2221 gcgataaagt cgaccacata gcgagtatag gctgttttat tttcacgacc acgcttgggt
     2281 acaggtgcta agtaaggcgc atctgtttcc accaacatct tgtccaaagg taactcttta
     2341 gctgcttctt ggaggtcagt tgccttcttg aaagtcacca ctcctgagaa ggaaatggtc
     2401 ataccaagat ccacaaactt ctctgcccac tcaagcgtcc ctgaaaatga atgcatgata
     2461 ccaccacgag gaccaacgcc ctcactcttg ataatctcat aggtatcttc cagcgcatca
     2521 cgggtatgga caacaaaagg caaatccaag tccttagata gctgaatctg acggcgaaaa
     2581 acctgctcct gcacctcttt gggcgctgtc atccaatggt agtctaagcc aatttcacct
     2641 aaagccacaa ccttggaatg ttttaactta tccaacaagt aagcctcaac ttcctctgta
     2701 taagtaccag cttctgtagg atgccaacca atagtcgcat agagctgctc atactcatct
     2761 accaacttca aggcatgctc aatcgtcggt ttatcaaaac caacaatatt catctgtgtc
     2821 acacccatct catcagccaa ggcaatttct tctgcctcac gacctgcaaa ttcttctaca
     2881 ttcaagtgtg tatgtgtatc aaaaatcatc tcttctaacc tcgttttcta tcttctatta
     2941 taccaaaaaa gaggaggggc acctaatttt tcggtttccc ctcctctctt caatagagag
     3001 ctattctgct atcttttcta tccgatattg cccatctcct gtcccacagt tagagacaga
     3061 agagattggc tacattgata gaatattcct attcagctaa agtatgtgat tatcttgggt
     3121 tctcactttt ctcacccatc actaggattg ataacttctc aaaatccccc ttataatcta
     3181 actttaagta gagaatagaa ttttctagct gttctatttt ctacgtctag tctttcttcc
     3241 tagcaaagca aaaccacttg ttgccgccgc tagaccaaga ctagcaagtg ctgcacttga
     3301 tgctgaacct gtgttaggta attgtttgcc agcaggtgac tggtaggtaa catcttgcat
     3361 tgttcctgca gttgcttgcg gtgtacttgt tgtcttagga gcagcattag gagccactgg
     3421 tctcttagga actgtaacca tggcaatggc ttgcactcca atcgtttctg cttccttcac
     3481 atcttccata gttttcgtat tttcaatctc tttgagagct gcttgttttt ctgcttccac
     3541 tcttgctaaa agttctgctt tttctttatc agaaagcggt gcgcctttga tttcatcctg
     3601 cttgtccttg gctgcttttt caatggcatt tttagctgat tctttttcag tagctaattg
     3661 cttagaagct tgtgattgag ctgagttttg tgaacctgtg tgagctgatc cacccgcctg
     3721 gctaccaccg ccttcattcg cattagcatc ttagcttcca gtattgcctc ccttatctcc
     3781 acctggtgta ttttctagct tatactctgg tgtagcttct tgagttacag attcaccttt
     3841 cgcagattgt tgaactgtat cttttcctag aatcgtcact actgaaccat ctgggaatgt
     3901 gattgttgct gtaccatctc cagctacatt gattgtcgct ccgtctaatg ctgaaccatt
     3961 tgcttgtaaa atagcaacct taactttttc ttcatctgtt aaatgagtta tatcttttac
     4021 atctactctt tgagcaggtg ctttaacagt tgatttttca gcagtcagtt tagtgctatc
     4081 ttgtgctttt ggatcttgtg ctaccaattg atttcctgga attgtaatag ctgaaccatc
     4141 tttaaagacg attgttgctg taccatctcc acctacttca actttatagt aaaataaaat
     4201 aataacagga caaatcgatc aggacagtca aatcgatttc taacaatgtt ttagaaacag
     4261 aggtgtacta ttctagtttc aatctactgt aacatcgctt ccgaatccat ttgcatctac
     4321 tgaacttttt tacaaaaata aaaatcagta gactcgagtt cctactgatt tttgatagaa
     4381 ttcgtttgga ttatgcagcc aaaactttgc gtcctttacg acgacgagct gccaatacgc
     4441 gacgaccatt tttagttgac atacggttac ggaatccgtg tttgcgcgca cgacgaagtt
     4501 tacttggttg ataagtacgt ttcacgatga atacctcctc atagattttg tattcgttta
     4561 gccggctata aagtgatcag ttactaaaca tactttacta ttctatctga ttctttcact
     4621 tttgtcaata gctctaggca aatgtttcca gctttttcga atgcaagccc tatctacgat
     4681 actgtttcaa gaaacgagtc atcttttctt ggatttgggc taactcatca acacgaggaa
     4741 ggtaaacgat acggaagtgg tctggttcct gccagttaaa gcctcgacca tgaaccaaga
     4801 gaaccttttc ctgcttcaag aaatcaagga caaactgctc atcatcatcg atacggtaca
     4861 tattgcggtc gatttttggg aagatataga gccccgcctt gggtttaacg gcagacaaac
     4921 ctggaatatc ttgaatggca ttatagatga aatttctttg ctcgtagatt cgtccaccag
     4981 gaagaagcaa ttcatcgact gattggtgac cccccaagga agtttgtacg acttgttggg
     5041 ccaaaacgtt agagcaaagg cgcatattgg acagcatatt gagcccttcg atatagccct
     5101 taacatgagt cttagggcca gacaagacca tccaacccac acggaaacct gctatgcggt
     5161 gggattttga cagaccattc atgctgacac agaagacatc tggtgccaag ctcgccacag
     5221 gcgtatgcac atgtccgtcc attaccatgc ggtcatagat ttcatccgca aagataatca
     5281 aatcgttttg acgggcaatc tcaataatct ccagcaagag ttccttagga taaagggctc
     5341 cagttgggtt atttggattg ataaggacaa ttgccttggt attggaagtg atttttgact
     5401 tgatatcgtc aatatctggg taccattcca cagcttcatc acagatatag tgaacggcat
     5461 ttcctccagc taggctgaca gccgccgtcc atagaggata gtctggcatt ggcactaaga
     5521 cctcatcccc attgtccaaa agcccctgca tggacatgac aatcaactca ctgacaccat
     5581 ttccaaggta aatatcatca atatctacgt ttgggaattt cttcagctgg caatactgca
     5641 tgatggcctt acgggctgag aaaatccctt tggagtcaga atagccttca ctatcacgcg
     5701 cattcataat caagtcatga atgacctcgt ctggcgctgt aaagccaaat tctgctggat
     5761 ttcctgtatt cagacgtaaa atcttttctc cattcgctcg catccgcatg gcttcttcca
     5821 atacaggacc acggatatca taagcaacat gctctaactt actagacttg ttatattctt
     5881 tcatcttgtt tcctcaattc atcaggatag taacattata ccactagaaa gagaatgcga
     5941 caagtttact gaaatgtgtt actagtttcg tatagaagaa aaaccttgct aaaggcgagg
     6001 ttaagaacgt agagatagca ggtattttgc aaagacacat cacataccaa aagataaacc
     6061 tagaaaaaag caagcctaga aaggagtcat aaagaagatt aaacatgagg aaaagccccc
     6121 atatcatcaa gtaatcccaa cgcccacttc gttttgcaac tagaaataag attagatggt
     6181 agagtacatg aaaaactaaa aatccaataa aacttggata aagaagcggt accagactct
     6241 ctaattgaca gatcatgatc acttctacca agactgaaac taggataatt ccataataaa
     6301 ggaaatttga tttttgaaaa gagtttttca tcattctccc ttccttcact tctttcctgc
     6361 tatcctttct tttattttat catatattta tatagagttt aagccctaat attcttttga
     6421 aaaatacttt ttttgcttta cttctatagt gaaaaggttt acaattatag taagaaaatt
     6481 tcaggaggtt tcattatggc acaacgttac caaaatatca tggtcgcaat cgatggttct
     6541 aaggaagcgg acttggcttt tgtcaaggga gttcattctg ctctacgaaa cgacgctaaa
     6601 ctcaccatcg cacatgtcat tgacacacgc gctctccaaa gcgtatccac ctttgatgct
     6661 gaagtttacg aagaactcca agtcgacgct gaaagtctga tgaaagagta cgaaaaacgt
     6721 gctaaagatg ctggggtagc agatgttcat atcgtcattg aaatgggaaa tccaaagacc
     6781 ctgctagcac gtactattcc agatgccgag gaagtggacc tcatcctcgt tggcgcaact
     6841 ggtctcaacg cctttgaacg cctcttggtc ggctcttcat ctgaatacat actccgccat
     6901 gctaaggtcg atttgctggt tgtgagagaa caagaaaaaa ccttataatc acaaagaaaa
     6961 ggagccccta gctccttttt gtttacgatt tatttctctc tttatggcgt tcgtaagcct
     7021 tgagctggcg ctgcagttcc tttttaatag caggttctgg agcatatttt tcttcccaat
     7081 tatctggttt taagatttta tgggtcactg gatcaaaatg agccttgcca tctggaaaaa
     7141 ttttccccat attggcctga tggacaatat caaaaatacg ttctgggtcc acccccatca
     7201 agacaaaact gccgtaggtg aagtaaagcg tgtcaatcaa ggcatccact tgccctatca
     7261 aatcttgctg agcaggtgtc ttcttggcta ctttatctgc tgccttatca agggcctgat
     7321 gaagttgcga tacagcttga ccaaaatctt cttcagaagg actggctgct cgaacaaact
     7381 ccaccaattc ttctatttta aaaccagccc tatgggttgc accctctaaa tcccaagctc
     7441 gaggttcttc ttgggttcgt tcatccatca tgtggtggaa ggtcttgacc ttattgaaat
     7501 gatagtcacg gctgacaaag actttttctg aagacaaaac accaaaatgt tccaaggcct
     7561 tgtggattcc atcttgttga ttgctagtgg taatgtgctt ggcagcttcc ttgacactgc
     7621 tactaccatt tcccatagcg accgacatac caacgccagc cagcatttca agatcattat
     7681 ctgagtcacc aaaagccatg acttggttga ggtcaaagcc atattctttc ccaactcggc
     7741 gaatgccttc taatttagaa tttccctgat tgatgacatc cgatgcaaaa ggattgctac
     7801 gtgtcaattt caagtcttca aaatcagctg ccgccttctc agattcttct ggtgtcatca
     7861 gcatcaaaac ttggtagata ggctgattca tcaggtgaag caggtcctct tccttttggg
     7921 gaacaacctt gctgaccatg cgattaaaag actgactcac cgtccgagtt aaaacagagg
     7981 gaacgaagcg actaattcgt tgggaaaaag aacccagacc aaaggacatg attttagaac
     8041 ccaacatggc atccttggtc cctagagcaa tctccgtgcc ctctttttta gcatagctaa
     8101 ttagatggcg caaatgtaac ttggaaatag ggctcgtgaa caagactctg tctttactaa
     8161 agatatactg gccattatag gttaccgcaa aatccagatc caaatcgtcc atcaattcct
     8221 taacaaaaaa aggtcctcgc cctgtcgcta cgccaactag taccccttgt tctttgacaa
     8281 tcttaatcgc atccttagtg gatttcaaaa cactcttgcg attgttgacc aaggttccat
     8341 cgatatcaaa aaaaacagct ttgacttcca tcctatccca atctcccctt ttgtgataca
     8401 atgattatac cacatttcag aaagagtgag taaatcatgc ctaagaaaat ccttgtttta
     8461 catacgggtg gaactatttc catgcaggcc gatgcttctg gcgctgttgt gacgagttca
     8521 gataatccca tgaaccatgt gtccaaccca cttgaaggaa tccaagtcca cgccttggac
     8581 ttttttaacc ttccaagtcc ctatatcaaa cccaaacata tgctggtcct ctaccagaaa
     8641 attaaagagg aagcagataa ctacgatgga gtggtgatca cacacggaac cgatacttta
     8701 gaggaaacag cctatttcct tgataccatg gaagttcccc atatgcctat cgttctaaca
     8761 ggagccatgc gtagctccaa tgagctcggt agtgatggtg tttataatta cctaagtgct
     8821 ttacgagtgg ccagcgatga cagggctgct gacaaaggag ttttggtcgt tatgaacgat
     8881 gaaatccacg ctgccaagta tgtcaccaaa acacatacga ctaatgtcag caccttccag
     8941 actccaacac atggccccct tggtctcatc atgaaacagg aaatcctcta cttcaaaaca
     9001 gctgaacctc gtgttcgctt tgaccttgat cacatacaag gtttagtccc tatcatctcg
     9061 gcttatgctg gtatgacaga tgagctgatt gatatgctgg atttagaaca cttggacggt
     9121 ttgattatcc aagccttcgg agctggtaat attcccaaag aaacggctca aaaattagaa
     9181 agccttctgc aaaaaggaat tccagtcgct ctggtatcac gatgctttaa cggtattgcc
     9241 gagcctgttt atgcatacca gggtgggggc gtacagttgc aaaaagcagg cgttttcttt
     9301 gttaaagaac tcaacgccca aaaagctcgc ttgaaactcc tcatcgccct caatgccgga
     9361 ctaacaggac aggctttgaa agactatatg gaaggctaat actcttcgaa aatctctgca
     9421 aaccacgtca cgtcgcctta ccgtatgtat ggttactgac ttcgtcagtt tcatctacaa
     9481 cctcaaaaac atgttttgag ctgacttcgt cagttctatc tacaacctca aaaacatgtt
     9541 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaac atgttttgag ctgacttcgt
     9601 tagtttcatc tacaacctca aaaacatgtt ttgagctgac ttcgttagtt tcatctacaa
     9661 cctcaaaaac atgttttgag ctgacttcgt cagttctatc tgcaacctca aagcagtgct
     9721 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaaca gtgtgttgcg cagcctttaa
     9781 tcagccgcct agtccgctct atggtattca ttaagtcaac atctcttgtt taagagcacc
     9841 aaatcaggaa atcttctcga ttccctgatt ttttctattt acgttttcgt gttgagctac
     9901 gttctgtcaa accatgaggt aagagaactt cacgttcttc caactcttcc ttatgcataa
     9961 tcttggtcaa catacgcata ctaatggcac caaggtcata aagaggttgg gcaatcgttg
    10021 tcaagtttgg acgggtaaag cgtgagattt gtgaatcatc actagtaata atttcaaaat
    10081 cttctggcac agaaacaccc ttatcagcca aaccgttcaa gactcctgct gccaactcat
    10141 cacctgtcac aactgctgca gttgcatttg atgaaatcaa acgctctgct aaggcgtaac
    10201 catcatcata gctatattta gattcaaata ccaaaccctc actataagtg attcctgctt
    10261 ttttcaaggt ttccttgtag ccaactaaac gaaccttacc attgatgtca tccactagcg
    10321 gaccgctaac gaaagcaata cgctcatttt ctttagcaag gtaactcact gcatcaattg
    10381 ttgcttgctt atagtcaata ttgacacttg gcaactggtg ctcaacatcg acagttcctg
    10441 cgagaacaat cggagtacgt gaacgcgaaa attctgagcg aattttatct gtcaagtgat
    10501 accccatata gataatgcca tctacctgct ttgaaaagag ggtattgaca acagaaactt
    10561 ctttctcgtt atcttcatcg ctattagcta ggacaatatt gtacttgtac atttctgcaa
    10621 tatcatcaat ccccttagcc aaactcgaaa aataaccatt ggtaatattt ggaatcacga
    10681 caccgacagt ggttgtcttt ttacttgcaa gaccacgcgc aactgcattt ggacgataat
    10741 ccaaacgatc aattacctct agcacttttt tacgggtatt ctcttttaca tttttattgc
    10801 cattgaccac acggctgacc gtcgccatgg aaacacctgc ttcacgagcg acatcataaa
    10861 tggttactgt atcatctgca ttcattcctt ttcctgtcct ttctatctca cacattcttt
    10921 tacaagtaga ggtactgatt gaagctctat atctacttac aaaagtgaag atgtgaaaat
    10981 ttcgttttca tatttctact tattccattc tatcactaat tgtaaacact ttcaagtgtt
    11041 ttttgaagat tgattgaaaa aatttcatag aaaacctagg tttagctcct tgctaccacc
    11101 ttagactaaa caaaaaggag gaaactaagc cctcctaaag ttatagtaaa atgaaataag
    11161 aacaggataa atcgatcagg acagtcaaat cgatttctaa caatgtttta gaagtagagg
    11221 tgtactattc tagtttcaat ctactatagg tattgttcca ttcactaccg tcaattttag
    11281 cacatagtct tcatgaaaat attatatcat cataaccaac cagattcttt cgcgatatta
    11341 gctgcctctg ttcgattacc tgcatctagt ttcgaaagaa tattggtgac atagtttcgg
    11401 actgttccgt tggatagata aagtttgtct gcaatttctt ggttagagaa gccctgagca
    11461 attcccttta aaactgcgat ttcttgctcc gttaatggat tgggatgcat catcaccact
    11521 tccatcaatt caggcgaata ctccttgcgt ccttcgagga cggtgtgcaa ggtttgcatg
    11581 aggtctgcaa tgtttctttc ttttaataca taagcatcta ctccagcctt gaccgcacgt
    11641 tcaaaatacc caggacgctt gaaggtcgtc accacaacca cctttgtttc aagcttttct
    11701 gctcgtatcc actccaagac ttcaagacct gtcttaacag gcatttctac gtcaaggatg
    11761 gcgatatcta cagactcctt ttctaatagt tggattgctt cttgcccatt cttggcttga
    11821 aagacagact ctacatccgg ttgaagcatg agcaactggc acatggcatc tcgcaacata
    11881 ctttgatctt ctgcgactaa tactttcatc tactttctct ccttataaag tagtcgaacc
    11941 tgcacttcag ttggatgttt ctgactgatt acacttactt ctcctgaaaa tggaaaaaca
    12001 cgatttcgga ctgtatggag ctcatccccg cttatagagg caaagccaca gccatcatct
    12061 ctcactgtta gaatgagttc tttctctgtc cgttctaatt tcaagtagac tttagacgct
    12121 ttagcatgtt tgatgatatt ggtcactaat tcaagcaaaa tcatggaagc cgttgactcc
    12181 aattcctgag ttaagctaga cttgtccaag tgattctcaa cttgaacctc aattccagca
    12241 atttctaaca tctttttcac agtctctagt tcggatgtca aagttctaga cttaagattt
    12301 tccacaatgg ttcgcacttc attcatggaa tccttgctga tctggtgaat ttcttttaat
    12361 tccttttcca cctgtggata agcctccatc tgaaataact gcaaggctaa atctgtcttg
    12421 acactcagca tagcaaaggt atgtcccaga ctatcatgca aatcctgacc gatacgacta
    12481 cgttcatttt cagcaagcaa tagatttatc tgagcatttt gcttgacctg agcttctttc
    12541 aaatcctcga caatacgaat ccgaaccaat ccaaaagtca ttaaatcgac aaaagtaaga
    12601 attacaagta gatagaatag aaactcaact tcgattctct gaaaaatcaa cagttgcccc
    12661 acaacaagga cttgagcaag aagaaaagtc cagacatgta aagactttaa actacgtacg
    12721 ctgaaatgat aacttaagag attggatagg aaaaagaaaa accagatata attaacagca
    12781 acaaaggcag tattcccaac tacataagtc agcatgaggc cccaatatag ccaagatagg
    12841 cgctggctct tagttgttaa aacacccaaa tatgccacta caaatagaat atcaatcaat
    12901 aaatgccagg cagaaagcca cccagtcact acagacagga tggggaaaat cataaaaatt
    12961 aaactgatcc aaaacatata atgtattctt ttcagtcttt caagcattaa gcattctcct
    13021 tatgaccttg aaggtaaata gtcaaaccaa ataaaactgc tgaaaaaaca agtagataaa
    13081 ctgtggctga tagattgatt ccaccctcat ttaagaaggt cttgattaat tccatcaact
    13141 gataacttgg taaacgcttc ccgattgctt gcatccagtc tggaaataaa gagacgggca
    13201 tccagagtcc gcctaaaaca gccaatccta gataaagaag attgcccacg acagacatca
    13261 gctgactagt tggtaacagc gtcaaggtta agccaagtgc tacaaaagca atacttccca
    13321 aaatcagcaa aatcgcagct ccaacccatt ttactagagg catgtctaca cctcttacaa
    13381 aatgtccaac tgtaaaaaca accagaattg agaccaaata atcaaccagc atacttgtta
    13441 tctttgatag ataatattct accatattta ctgggctatg acgtaatatt ttctgccagt
    13501 tgttcatctt gtcggtatgt aagacagctg ggaatgaaaa gatagcggtc gacatcatgg
    13561 aaaagccagt catggagatg aggtaatcac gcataaaatt cgcgggtcca cctggtgtgt
    13621 cctggtacat gccagaaaag aatagataga aggctgtcgg catccctact gacaatagat
    13681 aatagatcaa ttgtcgtttg gtcaataaaa attctatctt actaagtgct agccatcgtt
    13741 tcatcttagt tatctccctt ctgcgtttct tcaaagattg tatccaacaa actacgatta
    13801 ttaacttcaa tttcttgtat gccacatcct gcttgaacta acagttccca aaaagcatct
    13861 gcttcgcgtg tgactacttg tagagcatcc tgtttttgtg accagttttc aaccaagtta
    13921 gactgctcaa tgacttcctt gtatgccaga ggaaggataa aatgcttttc aattccctca
    13981 ctacgcatag ctagaggcgt cgtatcacga atcaactctc ccttatttaa gagcaaaatg
    14041 cggtctgcag tgtgttccac ttcctcaata taatgggacg aatagagaat ggtgactcct
    14101 tgcgctttta actcctggac aatttcccaa aaacgttgac gagttgaggt atccatggac
    14161 gcagtaggct catctaaaaa aacaatcttt ggtcgcccaa ttaaggtcaa gacgaaagaa
    14221 aagagacgct tttgaccgcc tgataatttt tctgccaact gttctttttg ttgcttgaca
    14281 aactgcaata gttgatcaac ttcctgattg ctcaaaggat ttggatagat acgttgaaag
    14341 aaagcaatca actctttaac ttttaatttc tgaacgatta cattttcttg agggaggtaa
    14401 gccctagtat agtctaactt agaactcgtc actggtaaac cttgaataga tacttgaccg
    14461 cttgtgacca gtttatctcc aagcaaacaa tctaagagtg ttgtctttcc ggcaccattt
    14521 ggaccaatta aggcaacaca ttcaccatcg ttgatttcaa aggagatgtt ccgcaaaatt
    14581 tccttgtctt ttattttctt actcaatttt tcaactttaa ccacagtcat gagactctcc
    14641 tttcaaccac tccattccca tagggaaaac gacgaaaatc ataaatccaa accccaaagc
    14701 accacgaatg aattggcgaa gcaaggcttg atcaaaccaa tcagtaaaca tttccactaa
    14761 ccataccaag agtgacaggc cgataaagaa atagatgatc cctttcttca ttcctcaaac
    14821 tcctttttca catctctgac taatttcaaa ccttctctaa caagccaaga catgattcca
    14881 aagccagcaa agatctttca aggaaaatgg tagaaatcct catccaatcc cgaaaacatg
    14941 agataggtca taactcctgc tactactaaa cttactgcga taatcatttt atttctcatc
    15001 tcttcttccc ccatttcata ctacaattat agtcttttaa aatcagagga ggcagaagct
    15061 tctgtcacta gaaaatatga caaatgtcat aaaaaattct gttcaaaaca agcaagatac
    15121 actatacaat aaaacacaat cagaaaaatc taaggcaact tcctcaaaag agatatcaaa
    15181 cccaattcac accataatgt aaactaatac ttatttaaaa tcaaaaagag tagaaatttt
    15241 tatcagacaa acacat
//
LOCUS       DAVLYV010000041        14943 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLYV010000041 DAVLYV010000000
VERSION     DAVLYV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14943
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.041"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(133..615)
                     /gene="greA"
                     /locus_tag="VYO70_001280"
     CDS             complement(133..615)
                     /gene="greA"
                     /locus_tag="VYO70_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1713509.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(686..2341)
                     /gene="mltG"
                     /locus_tag="VYO70_001281"
     CDS             complement(686..2341)
                     /gene="mltG"
                     /locus_tag="VYO70_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1713510.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVSTHPSQDVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     ISDTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2422..2916)
                     /locus_tag="VYO70_001282"
     CDS             complement(2422..2916)
                     /locus_tag="VYO70_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713511.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2913..3377)
                     /locus_tag="VYO70_001283"
     CDS             complement(2913..3377)
                     /locus_tag="VYO70_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713512.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3387..4721)
                     /locus_tag="VYO70_001284"
     CDS             complement(3387..4721)
                     /locus_tag="VYO70_001284"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1713513.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEVEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4732..5349)
                     /locus_tag="VYO70_001285"
     CDS             complement(4732..5349)
                     /locus_tag="VYO70_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1713514.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQLTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5397..8495)
                     /locus_tag="VYO70_001286"
     CDS             complement(5397..8495)
                     /locus_tag="VYO70_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1713515.1"
                     /translation="MAKLIPGKVRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINRQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADTNLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILADSHQIYVTSY
                     ATFRQDSELYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(8588..9754)
                     /locus_tag="VYO70_001287"
     CDS             complement(8588..9754)
                     /locus_tag="VYO70_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1713516.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNESKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(9762..10856)
                     /locus_tag="VYO70_001288"
     CDS             complement(9762..10856)
                     /locus_tag="VYO70_001288"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1713517.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVFAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(11014..12696)
                     /locus_tag="VYO70_001289"
                     /pseudo
     CDS             complement(11014..12696)
                     /locus_tag="VYO70_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(12868..14826)
                     /locus_tag="VYO70_001290"
     CDS             complement(12868..14826)
                     /locus_tag="VYO70_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1713518.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDANNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
BASE COUNT         4262 a         3339 c         2737 g         4605 t
ORIGIN      
        1 tgaaactgac gaagtcagta accatatata cggcaaggcg acgctgacgt ggtttgaaga
       61 gattttcgaa gagtagcaaa atggaaaaag gagtgagtga agcacatcgc ctccccactc
      121 ctttttctgt ttttaggctg ttttttcaac cttcaagatt tttacatcat agctaccaac
      181 aggcgtttca attgttgctg tgtcacctgt tttcttgcca atcaaggcct gcccaattgg
      241 gctttcattt gaaaccttac ctgcaaaggc atccgcacca gctgaaccta cgataatata
      301 aacttcttct tcgtcctcgc caatttcttg gatggtgact gttttaccaa tcgctacttc
      361 gtcctgggca actgcgtcgc tattgacgat ttcagcatag cggatttttg tttctaagct
      421 agagatttgt ccttcgacaa aggcttgttc atccttagct gcttcgtact cactgttttc
      481 tgaaagatca ccgtatgaac gggcaatctt aatgcgttct accacttctg gtcgacgaac
      541 caatttcaat tcttctaatt ctttttcaag tttctccttt tcctcaaggg tcataggata
      601 tgttttttct gccatttttc tcaactttct tctgataata ttttctaaag aaaattatgt
      661 gacgtatcac ataattttag tttgtttagt ttaatttgct gttgacatgt tcagcgacat
      721 tgcggtcgtg gtcttcttga ttgttagcat agtagacctt gccttctgtg acatctgcta
      781 caaagtagag gttatcgctc ttagtttgat tgatgcttga ctcaatcgca tccagacttg
      841 gactatcgac tggaccaggc atgagaccta catttttata aacattataa ggtgaatcaa
      901 tgttggtatc aatcgcaaca tcctcagcta gactgatatt ttgccccagt tttccttggg
      961 catacaagat tgcaatatta ctttgaagtg gcatatcacg atttaaacga ttgtagaata
     1021 cacctgcaat gagcttacga tcttctgtct tggcaccttc tttttcgacc aaggaagcaa
     1081 tggtcaacaa ctcattgaca gtcaaatttt tagatttgat agtactatag taaggagata
     1141 ggttcttatc catagcagct aacatctcat caatcaagct ctcaatagtt gtgctttcct
     1201 tgatagagta tgtagctggg aaaaggtatc cttccaaacg ataacgcgca ccgctgtctt
     1261 ttacaggcaa actttccagt aaagtaggat atttcgctac tgcttgactg ataaacgtct
     1321 catcttgaac tttagccaag aaagcctccg ctgtcaaaga ctctttgaag tcaccttgca
     1381 attgacccac agcttgagca atctgatcca aggtataacc ttctggaatt gtcaaagtcg
     1441 caagtacagg ttcttgcggt tcatctgttc cacctttttg caactctttg agtaagtctt
     1501 ctgtactcat actcttttgc aaattgtagt aacctgcttt caagtcggta taatttttat
     1561 acttggcata aaaactaaaa atcagaccat gctttaccaa accagctttt tcaagcgtcg
     1621 taccgatttc ttgaacgttt gaaccttctg gaattccaac cgtcacatat ttcttagaat
     1681 tagcatcgat aggtaacaaa gaatctagca cgtactggta accaaagtaa ccacctgctg
     1741 agagcaaggc aaggaataca aggaatgaaa tcacaaaagc tttcacatgt gacttcttct
     1801 ctttcttagg attaacgggt tttgctcctt ctctacggct acgacgtgat gtatcgctga
     1861 tgatatccac tgtttctgta gcaggcgtag gtgtttctgg acctgcttgt tctgcttttt
     1921 tctctgccgt cgtataggaa acagctaccc ttgttggggt ttcattgtat tctctttcaa
     1981 gtttcttagg tctaacagga cctggacctg gtcttgatcc actttcttcc gctggagaag
     2041 aaggtacatc ttgacttgga tgacttggaa caccaggagt ttctctttga atctcatctg
     2101 ctggagaagc tggtacatct tgacttgggt gagtagacac ggtaggagct tttctcataa
     2161 tctcctctac cgttgacaag gaatcagcca tgagttcttc agctgaaggt tcatttgcag
     2221 gagtgcgaac tactgcctca tcttctttca gaacttcatc atagcctttt actttttcta
     2281 aatctctcag aatctgctct ttaaagctta atttctcttc ttctcttgac ttttcactca
     2341 aaagtttttc ctccttgttg ataatccata atattatatc ttaaaagtcc aaaaaaagca
     2401 atctatgata cttttcctaa cttatttttt catttcccag accatatcat accatgtttc
     2461 ccctgcaaag gttgactggg actggccttc gttgacaaat ccatgttttt catagtaggc
     2521 gatgagatag tcatgacagg ttagattaat gccatctctc tcatcttcaa gagcaacttc
     2581 tttcaaggct gtcagcaatt tctgacccag tccgagtccc tgtgcctcct tggcaataga
     2641 cagacaggtc acagagatat aaccaccagg ctcatgacta tagtctttta tttcttctgt
     2701 aaaagactgg tcttgcagat ggcggtgcag gccaactggt ccttcgatat aacccatgat
     2761 tcttccttct ttttcagcaa ccagaaaaga ggtctgaatt tctctcaaat gtgcttcaaa
     2821 gacagaagga ggaatggctt cttcgaccga aaaattttca aattcaagtt caacaatccg
     2881 atccaaatct tctaatcttg cttgtctgat tttcattgtt cctccagata aaagggatta
     2941 aaccaaatca tactatagcc ctggctagtt gcatagagca aagttgcttc ttcatcaaca
     3001 aaaccgttca tttcaaaata ggaaagcagc tcatcaggac tctccaaacg aatccctttg
     3061 taatccagct caactgccac ctctttcaag gctgcaagaa gaagtgttcc caagccctgt
     3121 ctctgatggt cagactcgat gactaaagaa tgtactttta gatattgcgg attgtctgac
     3181 tggggacttg ataaaatata gcctaaaagt tgattttcat ccctagctag aagaaaggta
     3241 tccgcacact tacggatact ttcttctaaa atatgggaaa gttgctgctt ttcagctgga
     3301 aaagacgagg tctgaagtgc ccctatctca ggcaaatcag acttgcttgc ctgaatgatc
     3361 ttaattggaa tttccatggg aacatcctat tgaacattgc ttgtcaagtt agacaagaga
     3421 cgctcaaatg agtattcata ggtttggatg tctcctgctc ccataaagac gtaaacagca
     3481 ttgtcatggt ctaggagcgg agaaacattt tcaacagtaa tcacttggtg ttttttgttg
     3541 attttgttgg ctaggtcttc taccttaacg tcaccatgat ctacttcacg agccgagcca
     3601 taaatttgcg ctagataaac agcatctgct tggtttaaag catgggcaaa gtcgtccaac
     3661 aaggcaatgg ttcttgtaaa ggtatgcggt tgaaagactg ctacaatttc cttgcttggg
     3721 tatttctgac gagccgcatc caaggtcgca ataatttctg ttggatggtg ggcaaagtca
     3781 tcgataatca ctgtatcatt gacaattttc tcagtgaaac gacgtttaac accggcaaat
     3841 gttttcaagt gctcacgcac caagttcaaa tcaaatcctg ctgtgtaaag aagaccaata
     3901 acggctgtcg cattcatgat attgtgacga ccaaaggttg gaatgtggaa ttgccccaag
     3961 ttttgtccac ggaaatgaac ggtgaaggtt gaaccagtta ttgaacgaag aagatcacta
     4021 gctacaaagt cattgccttc aacttcaaaa ccataataat aaattggtgc atcagacgta
     4081 atcttacgca attcagcatc ttcaccatag acaaaaagac ccttggtgat ttgtttggca
     4141 tagtcgttaa aggcattaaa aacatcctcg agacttgtga aataatctgg atggtcaaag
     4201 tcaatgttgg tgataataga gtattctggg tggtaaggca tgaagtgacg ctcatattcg
     4261 tcagattcaa agacaaaata tttggcattg gccgaaccac gacctgtccc atctccaatc
     4321 aagaagctgg tatctgtaat gtgagacaag acatgagaca acatacctgt cgttgaagtt
     4381 tttccatgtg ctcctgctac tcccatgcta acaaagtcac gcataaagct acctagaaac
     4441 tcatggtaac gtttgtagct gataccattt tggtccgcat aggcaatttc gacgttgtta
     4501 tctggacgaa aggcatttcc agcgataatt tccatatcac cgtctagatt cttttcatca
     4561 aaaggaagaa tggtaattcc tgcctgctca agaccgcgtt gggtaaagta gtacttttca
     4621 acatctgatc cctgaacctt gtgccccatc tggtgcaaca tcaaggccaa ggcactcatc
     4681 cctgatccct taattccgat aaaatgatat gtctttgaca tgttttctcc cctattctgt
     4741 cattctggtc agattcaact cttgggcaac ccgacgttct tgttctgttt gtttactttt
     4801 tttattgtag atttggctct tctttagaaa atcataattg tttttctttg gagcaggtgc
     4861 tgacacttct tcattcttgg tagggataga atgaacttct tccgccaaga tataatgaga
     4921 ctgggtcaat ttttggctat atttgacaaa ttcaccagga ttttcctttt ggaaaggagt
     4981 tgtcagttga ttgccctgtc taactagact gggctgagaa tgacgtctcg caaggctgaa
     5041 atcctgagtt aggtagttag cagagcgttt ctttttcaag tccgcacgcg cttcttcacg
     5101 cgccacctcc gcatagctct ttccttcttt tttaacccct aaaggagcct ttttaggttt
     5161 ttcgacttgc ttttcaatcg gttttactgg tttttcttca gcaataggag cccattctaa
     5221 ataattttta tctcgatact cacccttgat attactgatc agatcagact catcatagag
     5281 attcatgact ggcatttcag tcaacatgac ctcgtcatct gacaccaatg gaaatcgttc
     5341 ttgtttcatt ttctatttcc tttcaacact tcattatagc gtattgtctt gatttttcaa
     5401 gtgctggctt cagaaattcc caaaatttct ctaatttctg ctagggtcag actaccacgt
     5461 gactctgtgc cgtccaatac ttgtgacacc agatgtttct tttgttcttg gagttcctga
     5521 attttttctt caatggttcc cttggtcacc aagcgataga cctcaaccgt ttcttcctga
     5581 cccatccgat gggcacggcc aatggcttgc gcttccaccg caggattcca ccaaaggtca
     5641 accaagatca ctgtatctgc acctgtcagg ttcagaccga ccccaccagc cttgagggaa
     5701 atcagaaagg catctctttc tccttggtta aaggccttgg tcatgtcttg tctttccttg
     5761 gctggggttg aacccgtaat tttaaaggaa gtcaggccca agtctggcag ttcttgttca
     5821 attttttcca acattccctt gaactgagag aaaatcaaga cacggtgtcc gccgtctgcc
     5881 acctgtacca gtaggtctcg gagactatct agtttgccgc tggctccctg ataatcttcc
     5941 ataaacaggg caggagtgtc acatatttga cgcaagcgca tcaaaccaga taaaatttcc
     6001 acacgacttc gctgaaattc ctgttctgac acttgagcca gatggtctcg catctgttgt
     6061 aactgggcaa ggtaaatagc cttttgctgg tcttccagtt catttttata aaccacctca
     6121 atcaagtctg gcaattcagt cagaacttct tctttcttgc gtcgcatcac gaaaggcttg
     6181 ataaactgag ccactcgctc tgctggcaat ttcataaatt ctttcttgct tggcaaaagt
     6241 ccgggcatga cgatttggaa aatagaccac aactcaccca gatggttttc aatcggagtt
     6301 cctgacaagg caaagaccga cggcaccaca aattgtctca aggtctgggc aatcttggtc
     6361 tgggcatttt tcatgacctg agcttcatct aagaaaagga agtcaaaggc catcccctga
     6421 taaagctcac tgtcctgacg gaaggtggca tagctagtca catagatttg atggctatcg
     6481 gcaagaatct cctcacgact tgctttcaaa ccatgaacaa cagccacatc caactgtggg
     6541 gcaaatttct gaaactcatc cgcccagttg taaatcaaac ccgacggagc gagaatcaag
     6601 actcgacttt cttttgtcac ttgactagtc aaaaaagcaa tggtctgaag ggttttccca
     6661 agtcccatat catcagccaa aatcccacca aaaccataat gatggagcat ctgcaaccag
     6721 ccaattccct tttcctgata atctcgcaag tcagccttga cctgagttgc ttgcaaagga
     6781 aagtcctctg gatgcgtcaa atcctgggcc agattctgga attcttgtga aaaagaaaca
     6841 cggtctcgcc cttcaaagag atgagctaaa ctgtaggcca aggatttccg agcctgcaag
     6901 gtcccatctt ttaattcaaa ttgccccagt tcctgtagat tttggcgaat tttcttggtt
     6961 tcttcatcga aaaagtaaac ttgattagac gaatcaatat aaaaatcctg attggcaacc
     7021 aaggcctgca tggcttggtc gatttcctcc tgggcaatat tttgaaaatc aaactggatt
     7081 tccaagagac ctcccttgga ggcaatctgc acctgaggac tcgctaggct ataaagctct
     7141 tctagtttat ctgataggtc aacatgcccg agtttttcaa agactggaat gatatcatga
     7201 aaaaaatgat agacagactc cgcttttaag gcctgacgcc aagattgaaa atcggcttca
     7261 aagcccgcag ccaaacagac ttggaaaatt ctttcttcta agtccgcgtc acttgaaaat
     7321 ggtaattctt ctagctcttg tcggctagat acctgtctat ttccataatc aaactgaatt
     7381 tctaaacgaa tccgattatc ttcttccctg tcaaagtaaa aagagggcgc aaaagttttg
     7441 atttgtagac gttctggagc tgaaacggtg cccatctgga taaaaagagt cagacaggag
     7501 gccaatttgt ctcgatcact gctatcaaat tgcaggtatt tctttccttg ttgacccaca
     7561 ggtaacactt taatttcctt gagaagacgc atctgctggt ctgttaaaaa ataaacctga
     7621 cctttatgga aaagtactgc tccctgataa aagacattga ccctaggact ctcactgatt
     7681 tccatttcaa aatagtccga gtattctgtt actgtaaagg caaatagatt ggtatcagca
     7741 tgcatatcct gaaaaagcag ggtttggtag ctatccactt gatggtcaaa ttgaaaatgg
     7801 ggcaaggcca tcagtaaatt cacaccctgc tcaaaaaagg tcagagggaa aaagaggtgc
     7861 cgaccttggt tttggaaaaa gaggtctgga accagccctt cctccgttag tccgtgcaag
     7921 aaagtcaaaa gttcttggct ggcatcatca aaggcttccc aagaaagaga ctcctcataa
     7981 atcttgccaa tcatatacga ctttctctgc tcgacaatcc ttaaaaaaag tgggatatcc
     8041 cgaatgacat agtatttttg tctattgatt tggccgattc tcagagtcca caagatatga
     8101 ttggttcctg cttccacctg acccacagct gataactcat aggcgcattc tgatttcgga
     8161 gataaaattc gattcaaaaa cttgccaccc aaggtcacct tggtttcaac ggcctctttt
     8221 tcttcatgac cttcttccag actccacaag atttcctgac cacgctcatc atttttcaga
     8281 aaatgctcta gcgctgccaa atgcacacag tagcccctct tttgaaaaga atcacaggca
     8341 caaaaaacca aatcatcctc taaactatag cgcagttctt cttctgcaac gcgagcgtag
     8401 agccgattgt tcttttcctt gatgatatca accttaccag tttcataaag ggcaacacct
     8461 tcgatacgaa ctttccccgg aatcaattta gccatatttt cacctttacc ttatcttttt
     8521 attataccat attttcgcct atgaaaatag ccttctagga agacttttct cctagaagac
     8581 tggattttta acgtttggca aaagtagcca caatccgctg acagacttcc tgcaacagag
     8641 atttgggcat agctatattg atgcgggcat ggagacttcc ttcctctcca aaatccaaac
     8701 cacggttgag gataaccttg gattcatttc tcaacaactc ttgcaatgtt tcatcagtca
     8761 ggtcataggc tgaaaagtca agccaaatca agtaggtacc ttgcggtttc atgaccttga
     8821 ttttagtctc ttttccaaat agatctacca cataattgat gtggtcttca aagacttgct
     8881 tgagttcctc tagccaatct ttaccgtatc gataggcagc ttctgtcgcc aaataaccca
     8941 agcctgaaat ttcatgctga ttattggcca acaggcgttt ctggaaagcc agtctcaact
     9001 taggattttc aatgactgca taggaatttt ttgttccagc aatattaaat gttttagtgg
     9061 cactgctcaa gacgatagca aaatttttga aggcaggatt gatggtattg aaagactggt
     9121 gtttgtgacc aaagagggtc aaatcttggt gaatctcatc cgaaactaac aaaacaccgt
     9181 gtttttggca gagttggcca atcttctcca acacttcttt ttcccaaaca cgtccaccag
     9241 gattgtgagg attgcaaaga atatagagtt taacctcctc ttccaccaaa tccttttcaa
     9301 gttggtcaaa gtcaatctca aacagaccat ccttttccac taaggaatta gtaatcaatc
     9361 tacgattatt caacttgaca ctgcgagcaa agggtgggta gacaggcgtg ttaattaaaa
     9421 ccgcctcgcc ttcttttgta aaggtttgaa tagctgttga gatggctggt accacaccct
     9481 cgataaagac aagagcctct ttgtcaaagt tgtaaccgta ttgtgtagct tcccactttt
     9541 gaacttcctt aattaagtct tcactggcat aggtataacc ataaaccagt tggtctgcgt
     9601 aagtttgcac ggcttggcgg atttcaggca agaccacaaa gtccatatcc gctatccaag
     9661 ctggtagaac ttcactatcc gtttctgttt ctttccattt ataggtatgg tgccctaaac
     9721 ggttgggcag gcttgtaaaa tcatattttc ccatctttgt cttatccttc taaggcttgg
     9781 cgcaaatctg caatcaaatc tctagcatcc tcaatcccaa tagacaaacg caagaggtca
     9841 tctgtcaaac cataagaatg gcgtacttct gctggaatat cagcatgagt ttgagtcgtt
     9901 ggataagtaa taagactttc cactccgccc aaactttccg caaaagagaa gaccttgaga
     9961 ctgttcaaaa tatgaggaat gcgtgtttca tcggctactt taaaggaaat catgcctcca
    10021 cgaccagtgt agagaacttc cttaactgct ggagaatcct tcaaaaaggc aaccacttct
    10081 tgggcgttag ctgttgagcg ctccatacga agagacaagg tcttgagacc acgaagcaac
    10141 tggtagctgt caaatggaga caagactgcc cctgttgtat taagattgta aaaaagcttc
    10201 tcgtatagtt ctaaactatt ggtcacaacc actccagcca agacatcatt gtggcctgct
    10261 agatacttgg ttgctgaatg gagaacgata tctgctccat cttcaatcgg acgttggtag
    10321 atagggctat agaaggtatt gtccaccacc actttggcac ccttagcatg agccaatttt
    10381 gctagttttt cgatatcaaa ttccaacatc aagggattgg ttggggtttc gatatagaga
    10441 acatccacat ccttttctaa ctcggcaatc aactcttctt ctgtattggc ataggtaaaa
    10501 tggaaacgac cttcctgctc cacttggtta aaccagcgaa aagaaccacc gtaaagatca
    10561 cgcactgcca agaccttact tcctactgga aagacgctaa aggccagtac aatagctgac
    10621 atccctgagc tagtcgctag ggcatagtct gctgactcaa tagccgcaaa gacttcctca
    10681 gccttactac gagttggatt tttagtgcgc gtatagtcaa acccagtaga tcgaccaaac
    10741 tctggatgct gataggtcgt tgaaaaatga agcggtgtca ccaatgcacc tgtcgcttca
    10801 tctgacttaa tacccgcctg ggccaaaatt gtgttaatgt gtaattcctt gctcatacaa
    10861 tacctccaaa tctatagtaa ctattgtacc acttattttc atcaactcat tttcttcttt
    10921 tcaagagcta gttatagttt caaactatat aaaaagggag tttttcctgc tccctttaat
    10981 agactataaa atggtgaatc tcaaaagaca ccttcactct atcatttgct cctgcacaaa
    11041 acgagcataa cgctcatgat tttccagtag ttccttatga gttcctgagc cagtgatttt
    11101 cctctcctct aagaagaaaa tacaatccac atcttttacc gttgacaaac gatgcgctat
    11161 aatcacaacc gtcttctcct ttaatgcaga atagaggata ctgataatcg catactcaga
    11221 atacgcatca agattagcag tggattcatc aaatataaga atttcagcat cttttaagta
    11281 ggttctagct atttgaagtc tttggctttg cccccctgac aagaatcgtc cgcgttcacc
    11341 aacttcagta tctagtccct ctttcatgga gcgaatctca tcacctagtg atactaagtc
    11401 tagcactttc atcaattcat catcagttac taagcgattc aaaccgagac aaagattgtc
    11461 acgaatactg ccagataaga ctgcattatt ttgtgaaacc caagcgattt tacttctcca
    11521 ttcttttaag ttaaaatcat atatacttga ttgctccatt agaatatctc ctgaaagcgg
    11581 tttataaaac tgctctaaca aacgcacaat cgttgatttt cctgatccag atggtccaac
    11641 aaaagcaatt ttttgcccct tgaaaattga acaagtaata tcctttaaga caggtcgatt
    11701 ttcatcataa ccaaaataga catggttaaa attcaaccct tgtcctgata ccgattttcc
    11761 tcactcaaat ttttctttag gaactgcaag caagttctcc agtgcaactg aagatccctt
    11821 gctcctagaa taaacagtta caaaattagc tatattacta ataggattaa gtaattgaaa
    11881 gaggtaaatc aaaaacgaaa ccaaggttcc cacagatata tatcctgcgc tgacccgata
    11941 acccccatag gttagcatca cagctatagt cgcaaagata aataagagag caaacggggt
    12001 ctcaaaagaa gtaaccctat ctgatttcag tgaattgttt tgtacccttt caatacaatt
    12061 atccaaaaca tcctgtacac ttttctctgc ttggttagtc ttaattaatt catgttcttg
    12121 aatcttttca gtcaattgcc ctgttaaaaa tccgatttcc tcctgtaaac gacgactata
    12181 cttttcactg atattggaaa ggggcaagat aataaacatc atacaaggaa gagtgatgaa
    12241 taaaagtaga gaaagattcc aatcaagact aaataagact acaatggaac caagtaccat
    12301 aactaaactc agaataatat ttgggaaagt cgtaattaaa aactcacgaa tgacactcgt
    12361 gtcattgaca atggcagaag tcaactcccc actttggctc ttatcaaaga aggatttctc
    12421 tgcataaatc aacccctcta tcactttttt cctgattttt gctatctttt tttcacccga
    12481 ttgactaaac agatagtaac caatagaaga aaacaaggct tgaccaataa aaatcaaaaa
    12541 cgattgaaat actttggaac ctatattttc aatagaactc ccatctatta aaccctttaa
    12601 gataagggga agcaacaaag caagtagact agacagaaca agtaagaaac tccccataat
    12661 caccttagta tctactctta ataattttaa tttcataaat actccttata atatttcaac
    12721 ggataaagtc gggaataact caatttgagg ataaaatcta ataaatcttc ctataacaaa
    12781 acgcataaca tctaggattt tatatacctg atattatgcg tttttaagca caaagacttc
    12841 ttacacaaac ttatctacaa ttagatttta tttgacatgt tttgccaatt cttcttgggc
    12901 ttttttattg gattcttctt tttctttcaa ccatttttct ctggcttttg catattcgtc
    12961 tgttgtgaca atcttatctt gtactttgag gtatttatat gattcaaccc cttttgtacc
    13021 ggttaaacca taggcagcag caaatggtac ggttcttctc aatgatggtg ttcccccacg
    13081 cgaaacactt ggaagaacta aagaactatc aatcaaccaa gcttgaatat cagcatattt
    13141 ctcataacgt ttggctggat cttgctcttt attagcttct tccaacattt gagtatagac
    13201 atccagtcca actgccttag ccttgtcatt ggcctcacca ggctctagtc caagattttg
    13261 cagaaatcct ccactattag tattaaaaat atcgagatag gttgacgggt cttgataatc
    13321 aggtccccaa ccgccatgat ataaatcata gtctttctga gcagctgttt gagcaaagta
    13381 gcctgaactg tcaaactcat ctgatgttaa ttgctgaatg tcaatcacta cattatcaga
    13441 acctaaaaca gattcaattg attgtttgat agaactaact ccttgtatgc ctactttatc
    13501 tgttacttcc acagtcttat ccaagtggat tgggaattga acaccctttg cttcgagttc
    13561 tttcttagct tccgcaaact tagccttggc tttctcagga ttgtagtaag ggtcttgacc
    13621 atccgcaaag ttgatacctt gccattcctt accatagttg accatcttag aggctacaac
    13681 ttcaccaaag tcttttccct tgatactgac aaagtttgga ggaaccacta ggttacgcaa
    13741 aatctttgtt gcaccttctt tcccttcaga ctgagcccca taagatgttc tgtcaaaagc
    13801 aaaattgata gcctgacgga agtttttatt gagaactgct tcctgggtcg atttcttttc
    13861 aatgtcactt gttttagaag tataattgta agacttccta tctaggttaa aattaaagaa
    13921 atatgaagtt gaattttgca tactatagat gatattgttt ttgtattttt ctttaatccc
    13981 ttcatagctg gagctgttag gaaaaagacg agccgtagta taagcaccag ctgtaaaatt
    14041 acgttccagt gattcttggt cgctaccatc atagtaggtc aatttcacat cgtctacaaa
    14101 gacattatta gcatcccagt aattagggtt tttcttatat tcaatagcag attttgagac
    14161 aagtgctttc atcaagaaag gtccattgta caaaatacta gatggatccg ccttcccaaa
    14221 atcatcccct tttgatttca ggaaatctgc attaacagga aaaagtatcg ttgcaagtgt
    14281 ttttgaattc cagtaaagtt ctggtttaac caaagtatat tgaaccgttt ggtcatcaag
    14341 tgccttgaca ccgacagttg aaaagtcgct tgttttacca gtgatatagt catccaaacc
    14401 agcaacagag tcctgcacta gatacaaggc ttctgatttt ttatcagctg catattgcaa
    14461 acctgtcaca aaatcctggg cagttacagg cgcatattct tctccctcag aagtaaacca
    14521 cttggcatcc ttacgaagtt tgtaggtata ggtcaaaccg tcctgagaaa cagtccaatc
    14581 ctctgctaat gatggaataa tattcccata ttggtcattt tctaataacc cgtctaccaa
    14641 atttgcaaca atatcggatg ttgctgcgcg gttttctgct agatagttca agctagatgg
    14701 atcacttgaa taaacatagt tgtaggtttt tgacgccgtg ctagaatttc cacacgcgct
    14761 caataaaact cctgtaccca ggacaagacc tgccaaggtt agatatttgc tcttagactt
    14821 tttcatttcc agaacctcct gctttaaata tttgttttat tatacaactc ttggtttgtt
    14881 tagtcaattg ttttttccaa aaaatcaaaa aaaacctcat tttctatttt tgaaagataa
    14941 ttt
//
LOCUS       DAVLYV010000042        14433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLYV010000042 DAVLYV010000000
VERSION     DAVLYV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.042"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            188..457
                     /gene="blpJ"
                     /locus_tag="VYO70_001291"
     CDS             188..457
                     /gene="blpJ"
                     /locus_tag="VYO70_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HEV1713519.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            526..756
                     /gene="blpU"
                     /locus_tag="VYO70_001292"
     CDS             526..756
                     /gene="blpU"
                     /locus_tag="VYO70_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HEV1713520.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            759..878
                     /locus_tag="VYO70_001293"
     CDS             759..878
                     /locus_tag="VYO70_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1713521.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDNLIRKIFSKFPKKDTDSTP"
     gene            1175..1339
                     /locus_tag="VYO70_001294"
     CDS             1175..1339
                     /locus_tag="VYO70_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713522.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            1336..1740
                     /locus_tag="VYO70_001295"
     CDS             1336..1740
                     /locus_tag="VYO70_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1713523.1"
                     /translation="MKTFLAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQLIAYIGAVLCLIIMLLLMWGLKYYKK"
     gene            1938..2742
                     /locus_tag="VYO70_001296"
                     /pseudo
     CDS             1938..2742
                     /locus_tag="VYO70_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            2892..3146
                     /gene="blpM"
                     /locus_tag="VYO70_001297"
     CDS             2892..3146
                     /gene="blpM"
                     /locus_tag="VYO70_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1713524.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            3162..3365
                     /gene="blpN"
                     /locus_tag="VYO70_001298"
     CDS             3162..3365
                     /gene="blpN"
                     /locus_tag="VYO70_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1713525.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            3609..3758
                     /gene="blpO"
                     /locus_tag="VYO70_001299"
     CDS             3609..3758
                     /gene="blpO"
                     /locus_tag="VYO70_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1713526.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            3862..3981
                     /locus_tag="VYO70_001300"
     CDS             3862..3981
                     /locus_tag="VYO70_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1713527.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            4767..5186
                     /locus_tag="VYO70_001301"
     CDS             4767..5186
                     /locus_tag="VYO70_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1713528.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKTNCLLNLTAVESDKVRS"
     gene            5201..5890
                     /locus_tag="VYO70_001302"
     CDS             5201..5890
                     /locus_tag="VYO70_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1713529.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFSGVMIWILVKTGHGYLFRIPRKKWLWYSILTVLVLVLQISFNVQIAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYVTLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     FLPSILFALPHFSSLPSLLDIFVFATFGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            5932..6165
                     /locus_tag="VYO70_001303"
     CDS             5932..6165
                     /locus_tag="VYO70_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713530.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLSSDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            6316..6927
                     /locus_tag="VYO70_001304"
     CDS             6316..6927
                     /locus_tag="VYO70_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1713531.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGDFFQNESALIIVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIVIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGLAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            7088..7882
                     /locus_tag="VYO70_001305"
     CDS             7088..7882
                     /locus_tag="VYO70_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1713532.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            7879..8514
                     /gene="trmB"
                     /locus_tag="VYO70_001306"
     CDS             7879..8514
                     /gene="trmB"
                     /locus_tag="VYO70_001306"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1713533.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            8640..9119
                     /gene="rimP"
                     /locus_tag="VYO70_001307"
     CDS             8640..9119
                     /gene="rimP"
                     /locus_tag="VYO70_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1713534.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEMISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            9163..10299
                     /gene="nusA"
                     /locus_tag="VYO70_001308"
     CDS             9163..10299
                     /gene="nusA"
                     /locus_tag="VYO70_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1713535.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            10321..10614
                     /locus_tag="VYO70_001309"
     CDS             10321..10614
                     /locus_tag="VYO70_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1713536.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            10607..10906
                     /locus_tag="VYO70_001310"
     CDS             10607..10906
                     /locus_tag="VYO70_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1713537.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            10923..13715
                     /gene="infB"
                     /locus_tag="VYO70_001311"
     CDS             10923..13715
                     /gene="infB"
                     /locus_tag="VYO70_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1713538.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            13966..14316
                     /gene="rbfA"
                     /locus_tag="VYO70_001312"
     CDS             13966..14316
                     /gene="rbfA"
                     /locus_tag="VYO70_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1713539.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         4475 a         2452 c         3207 g         4299 t
ORIGIN      
        1 aattaagata ttttagatac tcaagattat tgaaatctta gactaaaaaa taggtactgc
       61 tacctttaag aagatagtag acgtgtatac ttttttaaga aaatcaaaaa gatactataa
      121 aaaatctatt tgtttattga atttaagact ttggtaacaa attgaaaata aaaaggaggt
      181 attcatcatg aatacaaaaa tgttgtcaca gttagaggtt atggatactg aaatgcttgc
      241 gaaagttgaa ggggggtata gctccactga ttgtcaaaat gcactgatta caggagtcac
      301 tacgggaatt ataactggtg gaactggagc aggtttggcc actttaggag tagctggact
      361 tgctggtgca tttgttggag cacacattgg tgcaattggg ggtggattaa catgcttagg
      421 aggtatggtt ggtgataaat tgggactaag ttggtaagac ttatagtaaa attatgagga
      481 ttctattctg atttatttta gttttttaaa aaaggagtat ttgtcatgga tacaaaaatg
      541 atgtcacaat tttctgttat ggatactgaa atgcttgctt gcgttgaagg tggcggatgc
      601 aattggggag attttgccaa agcaggtgtt ggaggagcag cagcaagagg tcttcaacta
      661 ggcattaaaa caggaacatg gcaaggtgca gcaactggtg ctgcgggtgg agctatactt
      721 ggaggtgtag cctatgcagc aacatgttgg tggtaattat ggattttaaa agttttatta
      781 ttggtttagt agttggtata tttggtcctt atatggataa tttaattaga aaaatatttt
      841 caaaatttcc caaaaaggat acagatagta ccccctaaaa aataaatttc tcccctgtat
      901 ttgctagctt aatttacagg ggagtttctt taattttgtt tcgcctgtca aaatgatatg
      961 tgatagcagg atgaaaagtc tgcaaacaag ttggtatcaa tacaatataa agaggaaagt
     1021 atgtttttca tgcttgcttt tttaattttt acaattcaag aggttttgat gaccatttat
     1081 gatttgagtg atccaaggtc aaaataagtg ctatactagc agtgtaaatg ttcttgctca
     1141 actaagataa atcgcacatt taggaggaaa tcttatgaag aaaaaaatac tgattatttt
     1201 cgttctgtat ctaatcatgt ccatctttct atatccgctt agggagagtg cttggtatca
     1261 gctattttat accatagcct atgtgattgc agttatgatc tattttgcta tcaataaaaa
     1321 gaaaggagaa aagaaatgaa aacttttctt gctaaaaaac ggaacatctt ccttgcgaga
     1381 ttgttcttag gtcagttgcc cttgcttgtt tctacttatc tatttctatc tcgtcagttt
     1441 ttaaattttt ccgtagtttt ccaatttctt ttagtagtta ttaacttggc ttctattttg
     1501 gttactgttt atctcactag ggaaatgagg ataagagagt ttgaagatga tgatttggtt
     1561 agtcctagaa ccaatcaact tatgtttatc ggcttgacag gttttatgtc tattatttgt
     1621 ttgtatagag gtatcacagc aggagaatct tatcaacaac taatcgctta tattggcgct
     1681 gttctctgct taatcatcat gcttctgctc atgtggggct tgaagtatta taaaaagtag
     1741 agcttaatga agtataaaag aaaagggtaa gtgtttttgc aatacttacc cttttataat
     1801 agattccctg cgaaacaaaa tatggtatag tatattgaaa ctagaatagt acgcctctac
     1861 ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc gatttatcct gttcttattt
     1921 cattttacta tagttctatg aattatgaag caagtaaaca actaactgat gcacgattta
     1981 agctccttgt tggtgttcag cgtaccactt ttgaagagat gttagctgta ttaaaaacag
     2041 cttatcaact taaacacgca aaaggtggac gaaaacctaa attaagccta gaagaccttc
     2101 ttatggccac tcttcaatat gtgcgagaat atcgaactta tgaacaaatt gcggctgatt
     2161 ttggtatcca cgaaagcaac tttatccgtc ggagccggtg ggttgaagta actcttgttc
     2221 aaagtggtgt tacgatttca agaactcctc tcagttctga ggacacggta atgattgatg
     2281 cgacggaagt acaaatcaat cgccctaaaa aaagaattag cgaatgattc tggtaaaaag
     2341 aaatttcacg ctatgaaggc tcaggcgatt gtcacaagtc aagggagaat tatttctttg
     2401 gatatcgctg tgaactattg tcatgatatg aagttgttca aaatgagtcg cagaaatatc
     2461 ggacaagctg gtaaaatctt ggctgacagt ggttattaag gactcatgaa gatatatcct
     2521 caagtacaaa ctccacgtaa atccagcaaa ctcaagccac taacagttga agataaagcc
     2581 tgtaaccatg cgctatctaa ggatagaagc aaggttgaga acatctttgc caaagtaaaa
     2641 acgtttaaaa tgatttcaac aacctatcga aatcatcgta aacgcttcgg attacgaata
     2701 aatttgattg ctggtattat caatcatgaa ctaggattct agttttgcag gaagtctaat
     2761 gtaatttttg caattcaaga cgtttcgatg actattcaag atctggatga aaaattttaa
     2821 aaaacgatga tatactaaac ttgtcaaagt tacaacaaga caaaaattaa aaataaaaaa
     2881 gagtatttgt catggataca aaaattatgg aacaatttca tgaaatggat ataactatgt
     2941 tatctagtat tgaaggaggc aagaataatt ggcaaactaa tgtcttagaa ggtggtggtg
     3001 ctgcttttgg tggctggggg ctaggaacag ctatttgtgc tgcgagtggt gttggagcac
     3061 catttatggg agcatgtgga tacataggag ctaaatttgg tgtggatctt tgggcaggag
     3121 taacaggggc aacgggtgga ttttaataaa aggagacttg tatgaatact tattgtaata
     3181 taaatgaaac aatgctgagt gaggtttatg gaggtaattc cggaggagca gctgtagttg
     3241 ctgctttagg ttgtgcagcg ggtggagtga aatatgggag acttctagga ccatggggcg
     3301 ctgcaatagg aggaattgga ggagcagtgg tttgtggata tttagcctat accgctacat
     3361 cataaaaatg aaagctaaat tttatgaagg ctatattctt tattattctc ttcgcttttc
     3421 aaacctatct tatttatcta tccataagta tctctgataa aaaacaaaag actattgaat
     3481 taacatcatt gaattgtttt gtcatattat ttctaatcta cgataaatta atttttctct
     3541 ttattgccta tgtttttttg ataattttta tattaaactt gttccgtaac tgaaaagtga
     3601 catactatat gaatacaaaa atgatgtcac aattttctgt tatggataat gaaatgcttg
     3661 cttgcgttga aggtggagat attgattggg gaagaaaaat tagttgtgca gcaggggttg
     3721 catatggcgc aattgatggg tgtgcaacaa cggtttgaca tttctattgg gaccatttgc
     3781 tataggaata ggtgtaactg gtgctgcagg tggagctata cttggaggtg tagcctatgt
     3841 agcaacatgt tggtggtaat tatggatttt aaaagtttta ttattggttt agtagttggt
     3901 atatttggtc cttatatgga tgacttaatt agaaaaatat tttcaaaatc ttccaaaaag
     3961 gatacggata gtactctcta aaaaataaat ttctcccctg tatttgctag cttaatttac
     4021 aggggagttt ctttaatatt gtttcgcctg tcaaaatgat atgtgatagc aggatgaaaa
     4081 gtctacaaac aagttggtat caatacaata taaagaggaa agtatgtttt taatgcttac
     4141 tttttaaatt ttttaacaat tcaagatgtt tcgatgacaa ttcaagattt ggatgaaaaa
     4201 ttttaaaaaa caatgatata ctaaacttgt caaagttgca acgggtgctg ctacattatg
     4261 ttaaaaatcg ctcttgattg ataagctgga tttgttagga aaggagtaaa taattatgga
     4321 taagaaaaag atagtttcga ctataatatg tatagtattt cttgtagtgt cagtagataa
     4381 tttttttaga gacttaaccc cgttactgtt cattttaaat attattggcc tgtcttgctt
     4441 ttcagtgcta acatacatta acataaaaga gatactctta aatataagta aatgatgatt
     4501 tggttagtcc tagaaccaat caacttatgt ttatcggctt ggcagatttt atgtttgtca
     4561 tttgtttgta tagaggtatt tcagaaacag aattctatca acaactaatc gcttatatag
     4621 gtgtattctc tgcttgttta tcacgcttct gctcatgtgg ggcttgaagt attataaaaa
     4681 gtagctttct atccttttct gacaggatat ttgagtagaa ggctctatta aattgttagt
     4741 gaaataaatg aatggaggta tttaatatga aatacagatt attttttgtt attttcttga
     4801 gtagtgtgtt ggatattctt ttagggacat ttttacaaat ctctatcgta tctattgggt
     4861 ggcttgttct ttacagtggt ttgtttgaag taggagtttt ccttcttgct aataaagggg
     4921 tggcggtaaa aatcaaggaa gtagatattc gaaatcgctt taaatttatt tttggaaaaa
     4981 ccttatggtt tcaaattctt ttgctcatct ttttgataat caaactttat cttggtttgg
     5041 atgcgaggtt gattttattc tatggacata ttttcattgt ctttaatgcc ttaatgtatc
     5101 tattatctag tagtcaggtt agccttaaaa aaacaaactg tcttcttaat cttacagcag
     5161 tagagtctga caaggtgaga tcatagtagg gaggatttct atgaaaaagt atcaacttct
     5221 attcaaaata agtgcagtct tctcttactt atttttcgta tttggtcttt ctcagctgac
     5281 gcttatcgtc caaaattatt ggcaattttc ttcccagatt ggtaatttcg tctggattca
     5341 aaatatcttg agtttactat ttagcggagt catgatttgg attctggtta agacaggcca
     5401 tggttatctc tttcgcattc caagaaaaaa atggctttgg tattcgattt tgacagtatt
     5461 agtgctagtg ctccagatct cttttaacgt tcagatagct aaacatgttc agtcaactgc
     5521 tgaaggttgg gccgtattga tcggttatag tgggaccaac tttgctgagc taggtatcta
     5581 tgtaaccctg tttttcttga ctccactaat ggaagagttg atttataggg gattactgca
     5641 acacgccttc tttaagcatt cgcgatttgg ccttgatttg tttcttccgt ccattttatt
     5701 tgctcttcct catttttcaa gcctacctag tttgttagat attttcgtct ttgcaacatt
     5761 tggaatcatc tttgccggtt tgacccgcta taccaagagt atttatccat cctatgcggt
     5821 gcatgtgatc aataatattg tagcgacctt cccgtttttg ctcacttttc tacatagggt
     5881 cttggggtaa aaaaacaaaa ggcttgcttt tcagccatag aggaggtcat catgtataaa
     5941 cacttatttt tcctagattc caaaacttta gatcggttga caccctatat tctagtcttg
     6001 tcttctgaca ccattgcttt taatgttttt gtgctaacct ttgtatctgc ggtggttttt
     6061 aatttcctaa attccatgct agctttaatg gctatattca taggggctgg ctatgtggtc
     6121 ggattttggt tactaatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
     6181 ctgtacttga gtacggcaag gcgacgttga cgtggtttga atttgatttt cgaagagtat
     6241 aaaatggttt gttttggaaa ggctagaact aaagaatgac gtgtaggaag tctgtttgtt
     6301 gaggaggata attttatgga gttttttgat aaatttcatg ccttgtgttt tggattttta
     6361 gtactaataa ttgtcattac agttccttat acgattaacc atggggattt ttttcaaaat
     6421 gaatctgcat tgattattgt aagtcttctt gtaacctcgc tgagtgttgc ttatgctaga
     6481 aagtttgaaa tgatttcttt tgggatgtta agcaagaaac aacttttgct tttcattgta
     6541 atctttcttc taagtgtact tgagacgctg gtttatattc atttcttcgc tgtttcttct
     6601 ggctcagggg tccaacactt ggcggaagtc agcagaggaa tttccctgtc tttgattttg
     6661 actacctcag tttttggccc catccaggag gaactcattt tcagaggact tcttcaaggt
     6721 gcggtttttg acaattcttg gttagggctt gtgctaactt cctctctctt ttctttcatg
     6781 catggacctt ctaatgtccc ttcgtttatt ttttatctac ttgggggctt gttgctgggc
     6841 cttgcttata aaaagagcca aaacctatgg gtttctactc tagtccacat gttttacaac
     6901 agttggccac tcctatatta tttataaaaa ttatgaaaag gtaagcagaa gacggtgctt
     6961 acctttttca agctataatg atacccaagc caatccagag gcttttcttt gagaatcggg
     7021 ttagagtggt tgacgaatag gccaaaaact agtagaatag taaggaaact ttatacggag
     7081 gaaagaaatg gatttgggtg ataatgagct aacactgact cccatacctg ggaaaagtgg
     7141 caaggcttat atgggtagct atcctgatgg gaagcgcatc tttgtaaaaa tgaacacctc
     7201 tccaatccta cctggtctag ctagagaaca aattgctcca caattattat ggagtcgtcg
     7261 tttggcagat gggcgtgata tgtgtgctca agaatggttg acaggcaaga tattgacccc
     7321 ctatgatatg aatcgtaagc aaatcgtcaa tattttaacc cgtcttcatc gctcacgtcc
     7381 gttgatgaca caattgagtc gtttgggcta tgccatggaa acacctgtag atttactaca
     7441 gtcttggcaa gaaacggctc cagatgcttt gcgtaaaaat cattttatca gtgaagtgat
     7501 ggctgatttg cgtcagacta ttccaggatt tagagaggac catgcgacca ttgtccatgg
     7561 agatgtacga catagtaatt ggattgagac agatagtggc ttgatttatt tagtagattg
     7621 ggattcggtt cgcttgaccg atcgcatgtt tgatgtggcc catatgctct gccattatat
     7681 ttcagaacat cagtggaagg aatggttgac ctactacggt tacaagtaca atcaaacggt
     7741 attaagtaaa ttgtattggt atggtcaatt gtcttatttg agtcagattt ccaagtatta
     7801 tatgaaccaa gatttagaaa atgtcaatcg ggagattcat ggtttgcgtc atttccgaga
     7861 caagtatgga aagagaagat gagagttaga aatcgtaaag gggcaacaga attactagag
     7921 gcaaatcccc agtatgtggt cctcaatccc ttggaagcca aggcaaaatg gcgggacttg
     7981 tttggcaatg ataatcccat tcatgtggaa gttggaagtg gaaagggtgc ctttgtttca
     8041 ggtatggcca agcaaaaccc tgacatcaac tatatcggga ttgatattca aaagtctgtt
     8101 ttgagctacg ctttggacaa ggtgcttgaa gttggagtgc ctaacatcaa gctcttgtgg
     8161 gtagatggtt ctgacttgac tgactacttt gaagacggtg agattgatcg cttgtatctg
     8221 aacttttcag atccatggcc gaaaaaacgc catgaaaagc gtcgtttgac ctacaagacc
     8281 tttttggaca ccttcaaacg tatcttgcct gaaaatggag aaattcattt caagacggat
     8341 aaccgtggct tgtttgagta cagtttagtg agcttttctc aatatggcat gaaactcaat
     8401 ggtgtctggc tagatttgca tgccagtgat tttgaaggca atgtcatgac agaatacgag
     8461 caaaaattct caaacaaggg gcaagttatc taccgagttg aggcagaatt ttaagagata
     8521 gcctaaaatt aggctgtaca agtgcttttg ctttacataa gttggcaaac gtgctatact
     8581 gatagtaaga atatgaaaag tgaggcgggg aaatatcttc gcctcttgct tatgaggagg
     8641 tggacgcaat cgcaacaatc gtagaattag tcagagaagt tgtagaacct gtcatagaag
     8701 ctccttttga actcgtggat atcgagtatg gaaagattgg cagtgacatg attctcagta
     8761 tttttgtaga taaacccgaa ggaattacct tgaacgacac ggcagactta acagaaatga
     8821 tcagtcctgt cctagacacc atcaagccag atcccttccc agaacaatat ttcctagaaa
     8881 ttaccagtcc aggcttggaa cgtcctttga aaaccaagga tgccgtcgct ggagccgttg
     8941 gaaaatacat ccatgtcggg ctctaccaag ccatcgataa gcaaaaggtc tttgaaggaa
     9001 ccttgttggc cttcgaagag gacgagttga ctatggaata tatggacaag acgcgtaaga
     9061 aaaccgtcca aattccatac agtttagtat caaaagcacg tttagcagtt aaattataga
     9121 aaaagaaagg atagcttttg aggattcaaa agtgaagaaa acatgagtaa agaaatgcta
     9181 gaggccttcc gcattttgga agaagacaag ggaatcaaaa aagaagatat catcgacgca
     9241 gtagtagagt cgcttcgttc cgcttatcgc agacgctatg gtcagtcaga cagcgtagct
     9301 attgacttca acgaaaaaac aggtgacttt acagtttata ctgtccgtga agttgttgat
     9361 gaagtatttg atagccgttt ggaaatcagc ttgaaagatg ctcttgccat taattcagct
     9421 tatgaacttg gagacaaaat caagtttgaa gaagcaccag ctgagtttgg tcgtgtagca
     9481 gcccaatctg ccaaacaaac catcatggaa aaaatgcgca agcaaacacg tgccatcact
     9541 tacaatactt acaaagaaca tgagcaagaa atcatgtctg gtacagtaga acgctttgac
     9601 aaccgcttta tctatgtcaa ccttggtagc atcgaagccc aattgtcaaa acaagaccaa
     9661 attcctggag aagtttttgc ttctcatgat cgtatcgaag tttatgttta caaggttgaa
     9721 gacaaccctc gtggtgtgaa cgtctttgtt agccgtagcc atccagaaat gatcaaacgt
     9781 ttgatggagc aagaaattcc agaagtttat gatggaactg ttgaaatcat gagcgtggct
     9841 cgtgaagcag gtgaccgtac gaaggttgct gttcgtagcc acaatccaaa cgtagatgct
     9901 atcggtacaa tcgttggacg tggtggtgct aatatcaaga agattactag caaattccac
     9961 ccagctcgtt acgatgctaa aaatgaccgc atggtaccaa tcgaagaaaa tatcgatgtt
    10021 atcgagtggg tagcagatcc agctgaattt atctacaatg ccatcgctcc tgctgaggtt
    10081 gaccaagtta tctttgatga aaacgacagc aaacgtgcct tggtggttgt tccagataac
    10141 aagctttctc ttgccattgg tcgtcgtgga caaaacgtgc gcttggcggc tcacttgact
    10201 ggttatcgta tcgatatcaa gtctgctagc gaatttgaag ccatggaaga cgctgcttca
    10261 gtagagttgg aagtagaaaa cgatactgta gaagaataaa agctgctaga ggagggaaag
    10321 atgaaaacaa gaaaaatccc tttgcgcaag tctgttgtgt ctaacgaagt gattgataag
    10381 cgtgatttgc tccgcattgt caagaacaag gaaggacaag tctttattga tcctacgggc
    10441 aaggccaatg gccgcggcgc ttatatcaaa ctagacaatg cagaagccct agaggcgaaa
    10501 aagaagaagg tctttaaccg cagctttagc atggaagtgg aagaaagctt ttatgacgag
    10561 ttgatcgctt atgtggatca caaagtgaaa agaagagagt tgggacttga ataagcaaaa
    10621 gataagtaat ctcttggggc ttgctcagcg agcagggcgc atcatatcgg gtgaagaatt
    10681 ggtggtcaag gccattcaag acggcaaggc caagttggtc tttctagctc atgatgctgg
    10741 acccaatctg accaagaaga ttcaagataa aagtcattat tatcaagtag aaattgtaac
    10801 cgtgttttca acactggaat taagcatagc agtcgggaaa tcgagaaagg ttttggctgt
    10861 aacagatgct ggatttacaa agaaaatgag gtctcttatg gaatagaaga ggaggacatg
    10921 atttgtctaa gaaaagattg tacgaaatcg caaaagaact tggaaaagaa agtaaagaag
    10981 ttgtagcgcg tgcaaaagag ttgggcttgg atgtgaaaag ccactcatca agtgtggaag
    11041 aagctgtcgc tgcaaaaatt gctgccagct ttaagcctgc agctgctccg aaagtagaag
    11101 caaaacctgc agcaccaaaa gtaagtgcag aaaagaaagc cgaaaaatct gagccagcta
    11161 aaccagctgt agctaaggaa gaggcaaaac cggctgagcc agttgctccg aaaacagaaa
    11221 aagtagcagc gaaaccgcaa agtcgtaatt tcaaggctga gcgtgaagcc cgtgctaaag
    11281 agcaggcaga acgacgcaag caaaataagg gcaataaccg tgaccaacaa caaaacggaa
    11341 accgtcagaa aaacgacggc cgtaatggtg gaaaacaagg tcaaagcaac cgcgacaatc
    11401 gtcgctttaa tgaccaagct aagaagcagc aaggtcagca aaaacgtaga aatgagcgcc
    11461 gtcagcaaga ggataaacgt tcaaatcaag cggctccacg tattgacttt aaagcccgtg
    11521 cagcagccct aaaagcagag caaaatgcag agtacgctcg ttcaagtgag gaacgcttca
    11581 agcagtatca ggctgctaaa gaagccttgg ctcaagctaa caaacgcaag gaaccagagg
    11641 aaatctttga agaagcggct aagttagctg aacaagcaca gcaagttcaa gcagtggttg
    11701 aagtcgtccc tgagaaaaaa gaacctgcag tggatacacg tcgtaaaaaa caagctcgac
    11761 cagacaaaaa tcgtgacgat tatgatcatg aagaagatgg tcctagaaaa caacaaaaga
    11821 atcgaagtag tcaaaatcaa gtgagaaatc aaaagaatag taactggaat aacaacaaaa
    11881 agaacaaaaa aggcaataac aagaacaacc gtaatcagac tccaaaacct gttacggagc
    11941 gtaaattcca tgaattgcca acagaatttg aatatacaga tggtatgacc gttgcggaaa
    12001 tcgcaaaacg tatcaaacgt gaaccagctg aaattgttaa gaaacttttc atgatgggtg
    12061 tcatggccac acaaaaccaa tccttggatg gggaaacaat tgaactcctc atggtggatt
    12121 acggtatcga agcaaaacaa aaggttgaag tggataatgc tgacatcgaa cgtttctttg
    12181 tcgaagatgg ttatctcaat gaagatgaat tggttgagcg tccaccagtt gttactatca
    12241 tgggacacgt tgaccacggt aaaacaaccc ttttggatac tcttcgtaac tcacgtgttg
    12301 cgacaggtga agcaggtggt attactcagc atatcggtgc ctaccaaatc gtggaaaatg
    12361 gtaagaagat taccttcctt gatacaccag gacacgcggc ctttacatca atgcgtgcgc
    12421 gtggtgcttc tgttaccgat attacgatct tggtcgtagc ggcagatgac ggggttatgc
    12481 ctcagactat cgaagccatt aaccactcaa aagcggccaa cgttccaatc atcgtagcca
    12541 ttaacaagat tgataaacca ggtgctaacc cagaacgcgt tatcggtgaa ttggcagagc
    12601 atggagttat gtcaactgct tggggtggag attctgaatt tgttgaaatc tcagctaaat
    12661 tcaaccaaaa tatcgaagaa ttgttggaaa cagtccttct tgtagctgaa atccaagaac
    12721 tcaaggcaga cccaacagtg cgtgcaatcg gtacggttat cgaagcgcgc ttggataaag
    12781 gaaaaggtgc ggtcgcaacc cttcttgtac aacaaggtac cttgaatgtt caagacccaa
    12841 tcgttgtcgg aaataccttc ggtcgtgtcc gtgctatgac caacgacctt ggtcgtcgtg
    12901 ttaaagttgc tggaccatca acaccagtct ctatcacagg tttgaacgaa gcaccgatgg
    12961 cgggtgacca ctttgccgtt tacgaggatg aaaaatctgc gcgtgcagca ggtgaagagc
    13021 gtgccaaacg tgccctcatg aaacaacgtc aagctaccca acgtgttagc cttgaaaacc
    13081 tctttgatac ccttaaagct ggggaactca aatctgttaa tgttatcatc aaggctgatg
    13141 tacaaggttc tgttgaagcc ctttctgcct cacttcaaaa gattgacgtg gaaggcgtca
    13201 aagtgactat cgtccactca gcggtcggtg ctatcaacga atcagacgtg acccttgccg
    13261 aagcttcaaa tgcctttatc gttggtttca acgtacgccc tacaccacaa gctcgtcaac
    13321 aagcagaagc tgacgatgtg gaaatccgtc ttcacagcat tatctacaag gttatcgaag
    13381 agatggaaga agctatgaaa gggatgcttg atccagaatt tgaagaaaaa gttattggtg
    13441 aagcggttat ccgtgaaacc ttcaaggtgt ctaaagtggg aactatcggt ggatttatgg
    13501 ttatcaacgg taaggttgcc cgtgactcta aagtccgtgt tatccgtgat ggtgtcgtta
    13561 tctatgatgg tgaactcgca agcttgaaac actataaaga cgacgttaag gaagttacaa
    13621 acggccgtga aggtggattg atgatcgacg gctacaatga tattaagatg gatgatgtga
    13681 ttgaggcgta tgtcatggaa gaaatcaaga gataagattt tttgctcctt tcttaggtgg
    13741 tgagggacgc aagcaaaccg atggtttcat tgcttatttt tgagcctagg gtctcaaaaa
    13801 tcccccgtga tgggactgat aaatcagttc catcactttc accacggcga aagaagcaga
    13861 tgacttcaaa ttgaacttcg tttcaattta aactgaaaat caagaagttt aaaatagcta
    13921 ggtctgctgg cctagctttt ggttcaaagt agagaaagga atatcatggc aaatcatttc
    13981 cgtacagatc gtgtgggcat ggaaatcaag cgtgaagtca atgagatttt gcaaaagaaa
    14041 gtccgtgatc cacgtgtcca aggtgtgacc atcacagatg ttcagatgct gggtgacttg
    14101 tctgttgcca aggtttatta caccattttg agtaaccttg cttcggataa ccaaaaagcc
    14161 caaatcgggc ttgaaaaagc aactggtacc atcaaacgtg aacttggtcg caatttgaaa
    14221 ttgtacaaaa tcccagattt gaccttcgtc aaagacgagt ccatcgagta tggaaacaag
    14281 attgacgaga tgctacgcaa tctggataag aactaaagaa gaggggttgc ccctcttttt
    14341 tggtggagga aaataggttg aatttgaaat ggaaaaatat tcttttataa tagattgaaa
    14401 ctagaatagt acgcctctac ttctaaaata ttg
//
LOCUS       DAVLYV010000043        14349 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLYV010000043 DAVLYV010000000
VERSION     DAVLYV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14349
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.043"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..157
                     /locus_tag="VYO70_001313"
     CDS             <1..157
                     /locus_tag="VYO70_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1713540.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRKFENFKKRIFIALNIKKERT
                     KFVLSQA"
     gene            complement(221..1030)
                     /locus_tag="VYO70_001314"
     CDS             complement(221..1030)
                     /locus_tag="VYO70_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1713541.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            complement(1032..2381)
                     /gene="vicK"
                     /locus_tag="VYO70_001315"
     CDS             complement(1032..2381)
                     /gene="vicK"
                     /locus_tag="VYO70_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1713542.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     LLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            complement(2374..3078)
                     /locus_tag="VYO70_001316"
     CDS             complement(2374..3078)
                     /locus_tag="VYO70_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1713543.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            complement(3134..4309)
                     /gene="mutY"
                     /locus_tag="VYO70_001317"
     CDS             complement(3134..4309)
                     /gene="mutY"
                     /locus_tag="VYO70_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1713544.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATASEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            complement(4637..6307)
                     /locus_tag="VYO70_001318"
     CDS             complement(4637..6307)
                     /locus_tag="VYO70_001318"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1713545.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCALIDVPVELASVWADGVEGGVELAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQVVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            6537..7226
                     /gene="coaB"
                     /locus_tag="VYO70_001319"
     CDS             6537..7226
                     /gene="coaB"
                     /locus_tag="VYO70_001319"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1713546.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            7238..7789
                     /gene="coaC"
                     /locus_tag="VYO70_001320"
     CDS             7238..7789
                     /gene="coaC"
                     /locus_tag="VYO70_001320"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1713547.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            7773..8336
                     /locus_tag="VYO70_001321"
     CDS             7773..8336
                     /locus_tag="VYO70_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1713548.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            8618..9145
                     /locus_tag="VYO70_001322"
     CDS             8618..9145
                     /locus_tag="VYO70_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1713549.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            9157..9672
                     /locus_tag="VYO70_001323"
     CDS             9157..9672
                     /locus_tag="VYO70_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1713550.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(9669..10136)
                     /locus_tag="VYO70_001324"
     CDS             complement(9669..10136)
                     /locus_tag="VYO70_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1713551.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFTGTLQS
                     SEEGEVSWVQKDQIPNLDLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(10209..10697)
                     /locus_tag="VYO70_001325"
     CDS             complement(10209..10697)
                     /locus_tag="VYO70_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713552.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIDDLKVVGFISYGSFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LVKVALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            complement(10663..11226)
                     /locus_tag="VYO70_001326"
     CDS             complement(10663..11226)
                     /locus_tag="VYO70_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713553.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            complement(11236..13224)
                     /gene="uvrB"
                     /locus_tag="VYO70_001327"
     CDS             complement(11236..13224)
                     /gene="uvrB"
                     /locus_tag="VYO70_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1713554.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALIGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMTYNEEHDIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKVLD"
     gene            complement(13301..14254)
                     /locus_tag="VYO70_001328"
     CDS             complement(13301..14254)
                     /locus_tag="VYO70_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1713555.1"
                     /translation="MKNKRIFKDFQASKMNLNIYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
BASE COUNT         4262 a         3243 c         2550 g         4294 t
ORIGIN      
        1 ctattctaat gccaaactgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc
       61 ctttggtttt cggaaatttg aaaacttcaa aaaacggatt tttatcgccc tgaatatcaa
      121 aaaagaaagg acaaaatttg tcctttctca agcttagctt tttttcaacc cactacagtt
      181 gacaaagagc cataaaaaga acaccttctc agcgttcttt ctatatctct gtcaatggtg
      241 ttgcggtatc tggtgaggta tcataaacct taaagtctac tccgactccc agatcagctt
      301 gagccagctg attgaccatg gtcatatgag ccagttcctt gatattgttt tccttagata
      361 aatgcccaag gtaaatcttc ttagtacgat ttcctagcgt ccgaatcata gcttcagcac
      421 cgtcctcgtt agaaaggtga ccaaggtcag ataggattcg ttgtttgagt cgccaagcgt
      481 aagaacctga tcgcaaaatc tctacatcat ggttggcctc gataagatag ccatccgcat
      541 tttcgacaat gcccgccata cggtcactga cataacctgt atctgtcaaa aggacaaaac
      601 tcttgtcatc cttcataaag cgatagaact gcggtgcgac tgcatcatgg cttacaccaa
      661 aactctcgat gtcgatatct ccaaaagttt tggttttacc catttcaaaa atatgctttt
      721 gcgaagaatc caccttgcca agatatttac tattttccat agcttgccag gtcttttcat
      781 tggcataaag atccatacca tacttgcgag ccaaaacgcc tactccatgg atatgatctg
      841 aatgctcatg ggtaatcaag atggcatcca ggtcttctgg cttgcggtta atttcagcta
      901 gcagactggt aattttcttg ccagacaagc ctgcatccac taaaagcttc ttttttgagg
      961 tttccagata aaaagaattt ccactggaac ccgacgctaa aatactgtat ttaaagccta
     1021 tttcactcat tctagtcttc tacttcatcc tcccatactt cttctttcac tgcatcctta
     1081 tcataaggga gcacaatggt aaaggttgaa cccttgccgt attcactctt ggcccaaata
     1141 aagcccttat gttgtttgat aatttcttta gcgatagaca gtcctagacc tgtaccacct
     1201 tgtgcacgac ttctagcacg atccacacga tagaaacggt caaagatacg tggtaaatcc
     1261 tgcttaggaa tccccaaacc gtggtcagaa atggataaaa tcatctggtc ttcagttgtc
     1321 ttcattctga cagtgatttt acccccatct ggcgaatact taatagcatt atttaaaata
     1381 ttgtcgacaa cctgcgtcat cttatctgta tcaatttcca tccagataga attgatggga
     1441 taatctctca ccaactcata ttttttctcc ttttcctgtc ctttcatctt gtcaaaacga
     1501 ttgaggataa aggtaataaa agcagtgaag ttaatcagtt ccacatctag gagactggta
     1561 gcattatcaa tacgtgaaag atggaggaga tccgtcacca tgcgcatcat acggttggtc
     1621 tcatcaagag aaaccttgat aaagtctggt gctacagttt cacacaaagc cccctcatcc
     1681 aaggcttcaa gataggattt tacgctagtc agaggagtcc gtaactcatg gctaacattg
     1741 gaaacaaaga gtcttcgttc tcgttcttcc ttctcctgct ccgtcgtatc atgcaaaaca
     1801 gccaccaaac ctgaaataaa gccagactct cgacgtatca aggcaaagcg aactcgaagg
     1861 ttcaaatatt cgccattgat atcttgggaa tctagcaaca attctggact ttgggtaatc
     1921 aaatcacgca attcatagtt ttcttctatc ttgagcaatt ccaaaatgct tctattcaga
     1981 acatcttcct taaccaaccc cagttgcttc ttggctgtat cattaatcat gataatctga
     2041 ccccgacggt tggtcgcaag aaccccatct gtcatataaa acagaatact atttagcctc
     2101 ttactctctt gttctagatt ttcctgagtg agacgaataa cctccgacaa gtcattcaaa
     2161 ttattggtaa tattggtgat ttcagaccca ccttgcatat caagaacctt ggaataatct
     2221 cctgcaatca aatctttaac cttttgattg atttgcttca actgaatatt atcacgtcta
     2281 ttttccagta ataagagggt cacaacaagg atgaaaccta acaaaatcag gataaagata
     2341 aaatctctgg taaaaatggt ttgtttcagt aaatcaagca ttatttctca tgtaataccc
     2401 tacaccacgg cgcgtcaaga tatactctgg tcggctgggc gtatcttcaa tcttctcacg
     2461 cagacgtcgt acagtcacat caactgtacg gacatcacca aaatagtcat aaccccagac
     2521 agtctcaagc aagtgttcgc gcgtgatgac ttgacctgta tgcgatgcta aatgatacaa
     2581 aagctcaaat tcacgatggg ttaagtctag ttcttcgcca tattttttag ccacgtaggc
     2641 gtctggaaca atttctaaat ccccaatttg gataggttga ggtttactat ctgcttcctg
     2701 accatctact ggcataggtt gagaacgacg cagaagagct ttaacacgcg cctgcaactc
     2761 acgattggag aagggtttcg ttacatagtc atctgcccca agttccaaac cgataacctt
     2821 atcaaattca ctatctttgg ctgaaagcat aagaatgggc acactgcttg tcttacgaat
     2881 ggtcttagca acttctaaac catcaatttc tggaagcatc aaatccagaa taataatatc
     2941 tggttgctct gcttcaaatt gctctagcgc ttcacgacca ttaaaagcag ttacaacttc
     3001 gtaaccttcc ttggtcatat taaacttgat aatatccgag attggtttct catcatctac
     3061 aattagtatt tttttcatat gttcaccttt ttctccacta ttataccaaa aaaatagtca
     3121 gaagacacaa tagctagtct tggctactgt ctaagttggc ttgtgcataa gcctgccaga
     3181 ttttttgttg gggtttggca agtgggtaat tcttgaattc ttctggtgaa agccagcgaa
     3241 cttccctatc tgaaaaatca tggaagtcac tcacctgacc tgctacaatc tgcacatgcc
     3301 attttcgatg actaaaaaca tgctggactg tatcaaaaca aacatcaagc caatcaacat
     3361 ctaggtcata gtcctgctgg aaactctctt ctggactggg accaaagttc acactttctt
     3421 ccgcaacctg atgaaagagg tcaaactgct cttcttgcga aaagttatca acttctataa
     3481 aggggaaatg ccaaaaacct gccaagagct tttcgctttc atttttttca agtaaaaatt
     3541 gtccttgaga atttttcaca actaaggctt taagataaat aggaaccggc tttttcttag
     3601 gagatttaat tggataacgg tccatggttc cattctgata tgccgcacta aagtccttga
     3661 ctgggctttc ttcaggtctg ggatttacag gagactcaat atcagaccct aagtccatca
     3721 aggcttgatt aaaatcacct ggacgatccg gattaatcaa gatctccatc attgcctgaa
     3781 aaatttttcg attacttgga atcccaatat cgtggttgac ttcaaacaga cgcgccaaga
     3841 cccgcatgac attaccatct acagctggct caggcaagtt aaaagcaata ctggaaatgg
     3901 ctcctgctgt gtaaggtcca atccctttca agctggaaat tccttcatag gtatttggaa
     3961 attggccacc aaagtcagtc ataatctgct gggctgcagc ctgcatattg cgaactcgag
     4021 aataatagcc caagccctcc caagctttca gtaaactctc ctcagacgca gttgccagac
     4081 tttcgacagt tggaaaccag tccaggaatc tttcgtagta agggataact gtatccaccc
     4141 tggtctgctg aagcatgatt tcagataccc agatgtgata aggattttta cttctcctcc
     4201 aaggcaaatc tcttttgttt tcatcatacc aagcgagaag tttctcacgg aaagaaatga
     4261 ctttctcctc cggccacatg acgataccgt attctttcaa atctaacata tctctagtat
     4321 aacacagaag gtttcacctg tctttgtatc tgatttataa tattttcaat agatagtata
     4381 taacttttct atctacttat actcaatgaa aatcaaagag caaactagga agctagccgc
     4441 aggttgctca aaacactgtt ttgaggttgt ggatagaact gacagagtca gtatcatata
     4501 cctacggcaa ggtgaagctg acgtagtttg aagagatttt cgaagagtat aaatcttatt
     4561 gatgaactgc ttgcagtctg agaaaaaatg agcttggata ttatttccaa actcacttaa
     4621 agtcaatttc aatccactag aacaagccta gtacagttcc atcgctttca acatccatgt
     4681 tgagagctgc tggacgtttt ggaagacctg gcatggtcat aacatcacca gttaaggcaa
     4741 cgatgaagcc tgcacctaat tttggtacca attcacgaat ggtaatttca aagttttctg
     4801 gtgctccaag cgcatttgga ttgtctgaga aactgtattg agttttagcc atacagattg
     4861 gcaatttgtc ccaaccgttt tgaacgactt gagcaatttg tgtttgagct ttcttctcaa
     4921 agttcacttt gctaccacga tagatttcag tgacaatttt ttcaatcttt tcttggacag
     4981 aaaggtcatt atcatacaaa cgtttatagt tagctggatt ttcagcaatt gtcttaacaa
     5041 ctgtttcggc aagttctact ccaccttcta ctccatcagc ccagacacta gccaattcaa
     5101 ctggtacatc gattaaggca cagagttctt ttaaggctgc aatttcagct tctgtatcag
     5161 atacaaattc gttaatagct acaactgctg gaataccgaa cttacggata ttttcaacgt
     5221 ggcgtttcaa gttagcaaaa cctgcacgaa ctgcctctac attttcttca gtcagagcat
     5281 ctttagccac accaccattc atcttaaggg cacgaagggt tgcgacaata acaactgcat
     5341 ctggagatgt tggcaagttt ggtgtcttga tatcaaggaa tttctcagca ccaaggtccg
     5401 caccaaaacc agcttcagta acagtgtaat cagccaagtg aagggctgtt gtcgtcgcca
     5461 aaacagagtt acagccatga gcgatattgg caaatggacc accgtgtaca aaggcaggtg
     5521 taccgtaaat tgtctgaacc aagtttggct taatagcatc cttcaaaatc aaagccaagg
     5581 caccctcaac ctgcaaatca cctacagaaa caggcgtacg gtcatagcga taaccaataa
     5641 cgatattcgc caaacgacgt ttcaagtcct cgatgtccgt tgccaagcaa agaattgcca
     5701 tgatttctga agcaactgta atatcaaaac catcctcacg tggaataccg tttagaggac
     5761 caccaagacc aacagtcaca tggcggagcg cacggtcgtt caagtccaca acgcgtttcc
     5821 agaggatacg acgttgatca attcccagct cattcccttg gtgcaagtgg ttgtcaatca
     5881 aggcagaaag ggcattgttg gcagttgtaa tagcatgcat atctccagta aagtggaggt
     5941 tgatgtcttc cattggcaga acttgtgcat acccaccacc agcagcacca cccttgatcc
     6001 ccatgactgg accaagagac ggttcgcgga tagcaatcat ggttttcttg ccaatcttgt
     6061 tcaaggcatc cgcaagacca atggtaagcg tagactttcc ttcacctgca ggtgttgggt
     6121 tgatggcagt aaccaagatc aatttaccga ctggattgct ctcaactgcc cgaattttat
     6181 caaagctgag tttagccttg tactttccgt acaactccaa atcgtcgtaa gaaataccaa
     6241 gtttctctac aacatcaaca attggcttca actcaatact ctgtgcgatt tcaatatctg
     6301 ttttcattca aaattcctct aacctcttat atgataattc attatatcac aaaacaagat
     6361 ttttaacatc ctaaaactct ctaaacgttc gtaaatatct ctgtttttaa ggcttttaga
     6421 gtcctttctt aaattttata tggctttata gtttgaaact ataataaatc ttcgttttta
     6481 ccaaaaattt atcactttca ttttacttac cgcttatttt tgtgtacaat agtgctatga
     6541 aaattttagt tacatcgggc ggtaccagtg aagctatcga tagcgtccgc tctatcacta
     6601 accattctac aggtcacttg gggaaaatta tcacagagac tttgctttct gcagggcatg
     6661 aagtttgttt aattacgaca aaacgagctc tgaagccaga gcctcatcct aacctaagta
     6721 ttcgagaaat taccaatacc aaggaccttc taatagaaat gcaagaacgt gttcaggatt
     6781 atcaggtctt gatccactca atggctgttt ctgactacac tcctgtttat atgacagggc
     6841 ttgaggaagt tcaggctagc tccaatctaa aagaattttt aagcaagcag aatcatcagg
     6901 ccaagatttc ttcaactgat gaggttcagg ttttgttcct taaaaagaca cccaaaatca
     6961 tatccctagt caaggaatgg aatcctacta ttcatctgat tggtttcaaa ctgctggttg
     7021 atgttaccga agatcatctg gttgacattg cacgaaaaag tcttatcaag aatcaagcag
     7081 atttaatcat cgcgaatgac ctgactcaaa tttcagcaga tcagcaccga gctatatttg
     7141 ttgagaaaaa tcagcttcaa acagtccaga ctaaagaaga aattgcagaa ctcctccttg
     7201 aaaaaattca agcctatcat tcttagaaag gaaaactatg gcaaacattc tcttggctgt
     7261 aacgggttca atcgcctctt ataagtcggc agatttagtc agttctctaa aaaaacaagg
     7321 ccatcaagtc actgtcttaa tgactcaggc tgctacagag tttatccaac ctttgacact
     7381 acaggtactc tcacagaatc ctgtccactt ggatgtcatg aaggaaccct atcctgatca
     7441 ggtcaatcat atcgaacttg gaaaaaaagc agatttattt atcgtggtac ctgcaactgc
     7501 taacactatt gcaaaactag ctcacggatt tgcggacaac atggtaacca gtacagctct
     7561 agccctacca agtcatattc ccaaactaat agctcctgct atgaatacaa aaatgtatga
     7621 ccatccagta actcagaata atctgaaaac attagaaacc tacggctatc agctgattgc
     7681 tcctaaggaa tccctactag cttgtggaga ccacggacga ggagctttag ctgacctcac
     7741 aattatttta gaaagaataa aggaaactat cgatgaaaaa acgctctaat attgcaccca
     7801 ttgctatctt ttttgctacc atgctcgtga tacactttct gagctcactt atctttaacc
     7861 tttttccatt tccaatcaaa ccgaccattg ttcatattcc tgtcattatt gccagcatta
     7921 tttatggtcc acgagttggg gttacacttg gatttttgat gggattactt agcttgacgg
     7981 ttaacacgat tacgattcta ccgacaagct acctcttctc tcccttcgta ccaaacggaa
     8041 acatctactc agctatcatt gccatcgtcc cacgtatttt gattggttta actccttact
     8101 tagtctataa actgatgaaa aacaagactg gtctgatttt agctggagcc cttggttcct
     8161 tgacaaatac tatctttgtc cttggaggaa tcttcttcct atttggaaat gtttataatg
     8221 gaaatatcca acttcttctg gcaaccgtta tctcaacaaa ttcaattgct gaattggtca
     8281 tttctgcaat tctaacccta gccattgttc cacgactaca aaccttgaaa aaataaaaac
     8341 aatctccgct ttcaaacctg aaggtggaga ttttgctcta tacagtttct ttttaatgaa
     8401 atctttacta tagtaaaacg aaataagaac aggataaatc gatcaggaca gccaaatcga
     8461 tttctaacaa tgttttagaa gtagaggtgg actattctag tttcaatcta ctataacttt
     8521 taactaaaaa attatctata aacccaagta aattcttgct ttattgtctg gtttattgta
     8581 ccatactagt gtatatgcag ttaaaaagga gattcttgtg aatacacgga aaaagacaca
     8641 atttatgaca atgacagccc ttttaacggc tattgcgatt ttgattccaa ttgttatgcc
     8701 tttcaagatt gtcattccac ctgcttccta tactttgggg agccacatcg ctatttttat
     8761 agccatgttc ttgtcgccct tgatggcagt ttttgtcatc ctagcctcta gttttggatt
     8821 tttgatggct ggctatccca tggttatcgt ttttcgggct ttttcccata tatcttttgg
     8881 tgctttagga gctctttacc tacaaaaatt ccccgatacc ctagataaac caaaatcttc
     8941 ctgggttttc aactttgttt tggctgttgt tcatgccctt gctgaagtat tagcctgtgt
     9001 ccttttttac gcaacttcgg gtaccaatgt agaaaacatg ttctatgttc tatttgtgct
     9061 agttggattt ggcacaatta tccatagtat ggtagactat acattagcac tagctgtcta
     9121 taaagtgctt cgaaaacgcc gttaaaagga aaagctatga caaaagatcg caaacaagcc
     9181 ctgctccaac tcttgaaaga agctcctaaa gcccttaatg gccaaagttt ggcggaacat
     9241 tttcatgtca cacgtcaggt cattgtgcag gacattgcaa ttttgagagc cgatggcgct
     9301 cctatcctat ccaccaatcg tggctacatc tataagcaaa ttgataccaa tccttatgtt
     9361 cacaaacttt tcaaagtgaa acatgaagtt gaagaaatcg gtcaggaact ccttgctatt
     9421 gtcgataatg gtgggcatgt ccagaatacc ttgattgacc atcccgttta tggagaaatt
     9481 gaaaccttgc ttaaactgtc ttgtcgcaga gatgtgcaac attttctaga acaagtcgag
     9541 cattctgact ttagacctct atctgaattg acagatggca tccattacca cctagtcgaa
     9601 gccgaaacac aacaagacct ccactatatc gaggaggcct tggatcagct aggttattta
     9661 gtaaaagact agaagatttt cttttcccaa tcgtcttctg tacggcgagg gtagaaaaat
     9721 tcagacttat caggagcttc catcatctcc atcaagggta gcatatcata ggccagatcc
     9781 aagtttggaa tctggtcttt ttgcacccaa gaaacttctc cctcttctga agattgaaga
     9841 gttccagtaa actcagttgc cttataacaa atgacaatat agcgcccacc tgtatccagt
     9901 ggccaatttt taatgccgac aagttgagga ttttggatag tcaaccctgt ttcttcatag
     9961 atttcacgaa tgacagactc tgcgaaagac tcgccatttt ctacatggcc tcctggaaag
    10021 gcataaccag accagcgatt gttttcagga gcgcgatact gcatcaccac gcgctgagtt
    10081 tcgaggtctt caatcagaca aatgtttgtt aaaatcgtta attgtgaacg ggacataaat
    10141 ttactcgctt tctaatttat taaattttca gacttggtac cagagatatt agcttttact
    10201 ttttaaaatt atttagaata gaataccatc cgtttttcct ttataggctt tccaagttca
    10261 agtatctttt cttgtccatc aaaagtaaaa cccatttttt gatagaaagc aatggcgcgc
    10321 ttattatctt tcaataccca taagaaaatt tcagaaaaat gattaagagc agtcaaagct
    10381 actttcacta acttttgtgc gattcctttt ccataatagt cttttaaaac atataaagca
    10441 ataatttcac cagcttgaat agtctcatca cgaaagctgc cataactgat aaaaccaact
    10501 accttcaggt catctatcgc aatcaatgta ttttctggat acttttgact aaagaatcga
    10561 catctttcta atgtcattgt ttcctgaaat tctgcaggca aaaggtcatc ataagcctct
    10621 ctccacgttt gccagtgaac aagggattta ccttctatct cttcaggagt ttccattgat
    10681 ttgataataa ccgtcattta ttttctccca gtcttctctc aaaataccat atttaatact
    10741 atcaaaatat ttaccttgat aataacgaac ttttggaata tgagcttctt ttttcattct
    10801 taatttttca gcaagtttca tcataccaag attccctgac caagttgtca aacccagatg
    10861 ctccaactcc aagtaatcct gaaacgtcct atctatccac tgcaacatag caactttccc
    10921 aataccagtg ttccagaatt ttttatcata aataccaatt cccaattcca tccatcttgt
    10981 ttgtttacat acccaataac gcgaaacagt cccaactagt ttatcatcaa caaaaatacc
    11041 aaggcgattt gagttgctta aaatggattc tgatttttgt agttcgaatt ctttaaaatt
    11101 tgaaaaatac tgataatcat cataataggg agcatcatac tgtttccaaa ctggattaag
    11161 ttgtgaataa gccatttccc ataaagacat caaatcttct ctctttaact ttcttaaata
    11221 aatcatacta ttcccctaat ccaagacctt gacttccaac atcatatcac gaatctgagc
    11281 cgccagttca aagtcaagca cttcgacggc ttcttgcatt tgtttttcta gtttcttgac
    11341 gagttccttg cgctcttgtt tgttgaggct attgatgtcg acttccttgt cctcttcctt
    11401 agcaactgcc ttggtcacgg caatcaggtc acggatttct ttcttgattg tctgtggtac
    11461 gatatcatgc tcttcattat aggtcatctg aattttccga cggcgggcag tttcatcgat
    11521 agcacgttgc atagactggg taaccgtgtc tgcatacatg atgacatggc cttcactgtt
    11581 acgagcagca cgtccaatag tctggatgag tccacgttcg ttgcgaagga aaccttcctt
    11641 atcagcatcg agaatagcta cgaggctcac ttcaggaacg tcaattcctt cacggagcag
    11701 gttaattccg accaagacat caaagacacc caagcgcagg tcacggataa tctccgtccg
    11761 ttccaaggtc ttgatatccg agtgcatgta cttgaccttg atacccattt ccttgaagta
    11821 gtcggtcaag tcctctgcca ttttcttggt caaagttgtg ataaaggtac gctcattttt
    11881 ttcaacgcgg gcattgattt cacccaagag gtcatcaatc tgtcccatag tcggacggac
    11941 ttccacttca ggatccaaga gtcccgttgg acgaatgatt tgctcaatca ctgtctcagt
    12001 ctgttcattt tcatagtcac caggtgtcgc tgaaacgtaa acaatctgat gaacgtgact
    12061 ctcaaactcc tcccgacgga gaggacgatt gtccaaagca gacggcaatc ggaagccata
    12121 attaaccagc atttctttac gcgaacggtc tccattgtac atgcccttga tttgccccat
    12181 ggtcatgtga ctctcgtcaa tcataattaa gaaatcatct gggaagaaat ctaagagcgt
    12241 ataaggaggc tctccttcgc tccgtccatc catgtgtcgt gaatagttct caaccccatt
    12301 ggtatagccc atctcacgca acatttcgat atcatactct gtccgctgtt tcaaacgctg
    12361 ggcttcaagc agtttacctt ccttttcaaa gacagctaat tgttcttcca actcggcctg
    12421 aatctttgca atggcaactt ccatgtggtc gtcattggtc acaaagtgtg tcgctgggaa
    12481 aatcgctaaa tgatccactt ctcccaacac ctgacctatc agagcctcaa cttcacgaat
    12541 acggtcaatt tcgtctccaa aaaattctac tcgaaaggca tgttcatctc gggaagctgg
    12601 gaaaatctct accacatccc cacgaacgcg aaatcttccg cgttggaaat caatatcatt
    12661 acgttcaaac tgaatatcga ccaagtcatt caagagttta tcacgagaaa tctctagacc
    12721 aggacggaga ctaacgacac tatcagcgta ttccttgggc gaacccaaac catagataca
    12781 agagactgag gccacgacaa taacatcatt acgctccaaa agggctgagg tagctgagtg
    12841 gcgaagcttg tcaatctcgt cattgacaga actatccttc tcaatatagg tatcgctaga
    12901 agggacatag gcctctggct ggtaataatc atagtaggat acgaaatact caactgcatt
    12961 ttcagggaaa aattccttaa actccccata gagctgacca gccagagttt tattgtgggc
    13021 aataaccaga gttggtttat tgactttaga aatgacctga ctcatagtat aggtcttccc
    13081 tgttccagtc gcccccatca gaatctgagc tttttctccc ccctcaatgt tatccaccaa
    13141 ctgctcgata gcttggggtt gatctcctga tggttgatat tttgatacta gtttaaattg
    13201 attatctgta atgtgattga tcataagaat cctctctcca tgtgctattc ctattttagc
    13261 atacttatag tattttcacg aagaaaagga cggaccgaag tcacatcctt tcattttctt
    13321 tctttaattt attagcaaga tagacaatca gcagtagtaa taccagactt gtcataatgg
    13381 aaccttctat gccaaaagaa ccacctgata gccaatcttg attgccttgt ggtaaaaagg
    13441 tcatcagaga cgttcctgac ggttgaccac taactaaaat cccaaagaga ttaccctgag
    13501 caaaattcca agcaccatga atacctgcaa caccccaaac tgtatcagtt ttgagaaggt
    13561 aaagagccat ggcaactccg aataaaaaga gatttactag agatagaggg gtgagaccag
    13621 aattgcccat atgaagcagg gtaaagaaca ggctagatat aagaatagct agttttagat
    13681 tggttcttga ggccaattga ggaagtagcc aagcacgggc caccacttct tctgctgtcc
    13741 cctgtaaaat ccaaaatggg atagtaaaga cgacaaaggc aagagaataa ggattcaagt
    13801 gaatggattc caaacgatat tgacctaaga ccaccaaacc taacaaggtc agaagaaaaa
    13861 gtgccagacc tagactaaat cctttcagaa gattgctgag gaaattctct ctataaaatc
    13921 ccaaggttcg aataggtctt ttctcaactt ttcttgtcca tctgaacaca gtgttaagaa
    13981 taaaaccaaa ggccagtaat cctaaaatta aacgaaagtc acttgtttta tccgtcaagc
    14041 tctgatgcaa ggtctgcaag tagcttgcaa gattttgacc agcctctcca aaatttctag
    14101 caagtccgat gagagctaac aaactaatac catataaagt ataagccaca aactctccta
    14161 tgaagacaaa aacaaaggct aacaaggggc ttgtgtaaat gtttaaattc atttttgaag
    14221 cttggaagtc tttaaatatt cttttgttct tcatatcccc gtcctctttt cttctatata
    14281 tactattatt atatcacttt ttaatagcaa ttcaagaaag aaaaggaaaa atcaatgtta
    14341 tattttctg
//
LOCUS       DAVLYV010000044        14040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLYV010000044 DAVLYV010000000
VERSION     DAVLYV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.044"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            83..880
                     /locus_tag="VYO70_001329"
     CDS             83..880
                     /locus_tag="VYO70_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1713556.1"
                     /translation="MISSEFISKIEFACNKKEGLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1028..2288
                     /locus_tag="VYO70_001330"
                     /pseudo
     CDS             1028..2288
                     /locus_tag="VYO70_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
     gene            2384..3658
                     /locus_tag="VYO70_001331"
     CDS             2384..3658
                     /locus_tag="VYO70_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1713557.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKEHDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3668..4546
                     /gene="truB"
                     /locus_tag="VYO70_001332"
     CDS             3668..4546
                     /gene="truB"
                     /locus_tag="VYO70_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1713558.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            4787..5089
                     /locus_tag="VYO70_001333"
     CDS             4787..5089
                     /locus_tag="VYO70_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1713559.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            5197..5835
                     /gene="udk"
                     /locus_tag="VYO70_001334"
     CDS             5197..5835
                     /gene="udk"
                     /locus_tag="VYO70_001334"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1713560.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            5963..7303
                     /locus_tag="VYO70_001335"
     CDS             5963..7303
                     /locus_tag="VYO70_001335"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1713561.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLIIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESIVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            7281..7493
                     /locus_tag="VYO70_001336"
     CDS             7281..7493
                     /locus_tag="VYO70_001336"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1713562.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            7490..8365
                     /locus_tag="VYO70_001337"
     CDS             7490..8365
                     /locus_tag="VYO70_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1713563.1"
                     /translation="MKKQEKLALVESVLEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIASLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIVFCNQTLDQANEKLEEIAQQVPFE
                     TESIVSVVESLRING"
     gene            8358..9173
                     /locus_tag="VYO70_001338"
     CDS             8358..9173
                     /locus_tag="VYO70_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1713564.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            9166..9597
                     /locus_tag="VYO70_001339"
     CDS             9166..9597
                     /locus_tag="VYO70_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1713565.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            9604..11271
                     /gene="recN"
                     /locus_tag="VYO70_001340"
     CDS             9604..11271
                     /gene="recN"
                     /locus_tag="VYO70_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1713566.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSEIYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            11273..12001
                     /locus_tag="VYO70_001341"
     CDS             11273..12001
                     /locus_tag="VYO70_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1713567.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFCHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            12080..13903
                     /gene="lepA"
                     /locus_tag="VYO70_001342"
     CDS             12080..13903
                     /gene="lepA"
                     /locus_tag="VYO70_001342"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1713568.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         4153 a         2559 c         3348 g         3980 t
ORIGIN      
        1 acttttatga aatacagaaa acgcttccta aaacctaaag tttgtgatat aattatcaag
       61 aaaaaagatt taggagttta ttatgatttc ttcagaattt atctcaaaga ttgaatttgc
      121 ttgcaataag aaagaaggtc tttatagtca aagcaaattt aagtatgcga ttcgttcgat
      181 gttcgcaggt gcatttttaa ccttcagtac tgctgcaggt gcagttgggg ctgacttgat
      241 taataaaatt gcaccaggta gtggacgctt cctctttcca ttcgtttttg cttggggctt
      301 ggcctacatt gtttttttga atgccgagtt ggtcacttca aacatgatgt tcttgactgc
      361 tggtagtttc ttaaaaaaaa tctcttggag aaaaacagct gagattttac tatactgtac
      421 cttgttcaac cttatcggag ccttgatagc agggtggggc tttgctcatt cggcagccta
      481 tgcgaatctg acacacgata gtttcatctc aggtgttgtt gagatgaagt taggccgctc
      541 aaatgaattg gtcttgcttg aggcgatttt ggcaaatatt ttcgtaaata ttgcgattct
      601 gtcatttatt ttggtcaaag atggtggtgc caaactttgg cttgtgttgt cagctattta
      661 catgtttgta ttcttaacaa acgagcacat tgcggcgaac tttgcttctt tcgcgattgt
      721 gaaattcagt gttgctgcgg attcaattgc caacttcggt gttggaaata tgcttcgcca
      781 ctggggtgtg actttcatcg gaaactttat cggaggaggc ctcttgatgg gtcttccata
      841 tgccttcctc aataaaaacg aagatactta tgtagattaa gaaaatgagc acgattgagt
      901 cgtgcttttt tcattttcaa aataaggtaa cagctatttc ttatatcaaa atatagaaaa
      961 ctgatatttg taaactataa ctcaaggtgc tacaatatcc ttaataaaat aatatggagg
     1021 tcaccttatg acttgtgatt ttaaatttga aactctacaa ctacatgctg gtcaagttgt
     1081 ggctccagct actaagtctc gtgcagtgcc gatttaatca aacaacattt tttgtttttg
     1141 atgacacgta ggaaggtgcc gatatgtttg ccttgaggaa accagggaac atttatactc
     1201 gtatcaccaa tcctacaaca gctgcccttg aaggtggtgt tgaagcgcta gcaacagcat
     1261 caggtatgac tgcagtgact tatacgattt tggcgcttgc ccatgctggt gaccatgtag
     1321 tggctgcttc gactatttac ggtggaacct tcaatctttt gaaagaaccc cttcctcgtt
     1381 atggtatcac aacaaccttt gtcgatattg ataatttgga ggaagtagaa gcagctatca
     1441 aagacaatac caagcttgtc ttgattgaaa ccttgggtaa ccccttgatt aatattccag
     1501 acctggaaaa actggcagag attgctcata aacatcaaat cccacttgtg tcagacaata
     1561 cttttgcaac accttatttg attaacgtct tctctcatgg cgttgacatt gccattcact
     1621 ctgcgactaa gtttatcggt gggcatggta caactattgg aggaataatt gtcgatagtg
     1681 gtcgttttga ctggacggct tcagggaaat tccctcaatt tgttgacgag ggtccaagct
     1741 gccacaattt gagctatact cgtgatgtgg gtgcagcagc ctttattata gctgttcgag
     1801 ttcaattgct tcgtgataca ggtgcagcct tgtcaccatt caatgctttc ctcttgctac
     1861 aaagacttga aaccctttca cttcgtgtgg aacgccatgt acaaaatgct gagacaattg
     1921 ttgattttct tgtcaaccat cctaaggtag aaaagataaa ttatccaaaa cttgcagata
     1981 gtccttatca tgccttggct gagaaatact tgccaaaagg tgtcggttca atctttacct
     2041 tccatgtcaa aggtggcgag gaagaagcac gcaaggtcat tgataatttg gaaatcttct
     2101 cagaccttgc taacgtggca gatgccaagt ctcttgttgt tcatccagca acaaccactc
     2161 atggtcaatt gtcagaaaaa gacctagaag cagcaggtgt cacaccaaat caaatccact
     2221 tgtctatcgg acttgaaaat gtagatgatt tgattgaaga cttgcgcttg gccttggaaa
     2281 aaatttaaag aaaaagaaga taaacagtgg ttttcgactc gctgtttttg attttccttc
     2341 atgcatgata taatggttac agaagtctag aaagaggaat gtaatgaacg aaatcaaatg
     2401 tcccaactgt ggggaagtct ttacagtaaa tgaaagccag tatgctgaac ttttgtccca
     2461 agtgagaacg gcagagtttg ataaggaact acacgatagg atgaggcagg aactggcctt
     2521 ggctgagcaa aaggctatga atgagcaaca gactaaactg gctcagaagg atcaagaaat
     2581 tgcgcaatta cagagtcaga tccaaaactt tgatacagaa aaagaattgg ccaagaaaga
     2641 agttgaacag acaagccatc aggccttatt ggctaaggac aaggaagtac aggctttgga
     2701 gaaccaattg gccactttgc gtttggagca tgaaaatcaa ctgcaaaaga ctctttctga
     2761 cctagaaaaa gaacatgatc aggtcaaaaa tcagctcctc ttgcaagaaa aggaaaatga
     2821 gttgtctttg gcttctgtta agcaaaacta tgaagcccag cttaaagcag ctagtgaaca
     2881 agtcgagttt tataagaatt ttaaggctca acaatctaca aaagcgattg gggaaagcct
     2941 agaacagtat gcagagagtg agtttaacaa ggttcgtagt ttcgcctttc caaatgctta
     3001 ctttgagaag gataacaagg tctcttcgcg tgggtctaaa ggggacttta tcttccgtga
     3061 gtgtgatgaa aatggagttg aaatcatttc tatcatgttt gagatgaaaa acgaagcgga
     3121 cggaacagag aagaagcaca agaatgcaga tttttacaag gaattggaca aggaccgtcg
     3181 ggagaagaac tgtgagtatg ccgttttggt gaccatgctt gaggccgata atgactactt
     3241 taacacaggg attgttgacg tcagccacga gtatgaaaaa atgtatgttg ttcgtcctca
     3301 attctttatc cagttgattg gtctcttacg taatgcggcg ctaaattccc taaaatacaa
     3361 gcaggagttg gccttggttc gcgagcaaaa tattgacatt acgcattttg aggaagattt
     3421 ggatgccttt aagctagctt ttgctaagaa ctataattca gcttcgacta actttggaaa
     3481 agctattgat gaaatcgaca aggccatcaa acgcatggaa gaggttaaga aattcctgac
     3541 cacatctgaa aaccaactcc gtctagctaa caacaaattg gaagatgtct ctgttaaaaa
     3601 attgacccgg aaaaatccaa caatgaaagc gaagttcgaa gcactgaagg gggagtagaa
     3661 agcaaaaatg aacggtatta tcaacttaaa aaaagaagca ggaatgacct cgcatgatgc
     3721 ggtttttaaa ctgcgtaaga ttttgggaac caagaaaatt ggtcatggtg gaaccttgga
     3781 tccggatgtg gtgggtgttt tgccgattgc ggttggcaag gcgacacgca tggtcgagtt
     3841 tatgcaggac gagggtaaga tctatgaggg ggaaattact ctgggttatt ccacgacgac
     3901 tgaggatgct agtggggaag tgatcgcaga aacccctgtt ttgtctccct tggatgaaaa
     3961 gcttgttgat gaagcgattg ctagcttgac tgggcctatt acccagattc cccctatgta
     4021 ttcggcagtt aaggttaatg gtcgcaagct ctatgagtat gcgcgtgctg gtcaggaagt
     4081 ggagcgtcca gaacgtcagg tgaccattta tcaatttgag cgaacaagtc cgatttctta
     4141 tgatggccaa cttgcccgat tcacttttcg tgtaaaatgc agtaaaggga cttacatccg
     4201 tactttgtca gttgatttgg gtgaaaagct tggttatgcg gctcatatgt cccatttgac
     4261 tcgtactagt gctgctggct tacaattaga agacgctctt gccttggagg aaattgctga
     4321 aaaagtagag gctgggcaat tagattttct ccatccttta gagattggga caggtgacct
     4381 tgtcaaagtt ttcctaactc cagaagaggc tacagaagtt cgctttggtc gttttattga
     4441 gctagaccaa acggacaaag aactggctgc ctttgaagat gataaattgt tagccattct
     4501 agaaaaacgg ggcaatctct ataagccaag gaaggttttt agctagatcg tttaggaata
     4561 aaaatcgggt gatagataac aattgcttga taaaacccca tactaatagt agaatggttt
     4621 tgggaattat aatattccaa ttgttgcgag ttgtaggtac tcaaataatc tatatagaaa
     4681 tttagaggtg tgaaatgaag caatttaaaa ttctttcaga taaatattta gagtccatta
     4741 caggttctgg tgggaactta ggcccaggat ttggtgtgat aattccatga tgcgaaatga
     4801 gtttcgagaa agggtggagc aacttcttca acaaaaagaa ataaatgaaa atagtgagtt
     4861 gagtcacctg tttcgtcttg ctatacaaaa tttagacaga aatgaaaaat accaaacggt
     4921 catggccaat ttgagtcaag ggttgtcact ttacctcatg acgcatcatt accaggcacc
     4981 taagtctgtc attgattttg gtttatggat tgccaaagct cctagccagg aaagagggag
     5041 actggctttc ttgcagatgc ttgctcaaac cctacagggc tttcgttaaa actgtcgaac
     5101 atcagaggga agactctggt gttttttgct gatgagacta taaattttgt aaaaaatgtg
     5161 atagaataga cgacggataa aataacggag gatagcatgc aaaatagacc aatcattatt
     5221 ggagtgacag gtggttctgg tggtggtaaa accagtgttt caagagccat tttatcgcat
     5281 tttcctgatg aaaagatttc catgattgag catgattcat actacaagga tcagtctcat
     5341 ttgacctttg aagagcgtgt caaaaccaac tatgaccatc cttttgcctt tgatacagac
     5401 ttgatgatcg agcagattaa ggaattgttg gcagggcgtc cggtggacat cccgacctac
     5461 gactatacag agcatacacg gagtagcaag acctatcgtc aggagcctca agatgtcttt
     5521 atcgttgagg gcattttggt cttggaggac aagcgtctgc gcgatttgat ggatatcaag
     5581 atttttgtgg atacggatga cgatgtgcgt attattcgtc gtatcaagcg tgatatggag
     5641 gaacgtggcc gtagccttga tagcgttatt aaccagtact taggtgtggt caaaccaatg
     5701 taccaccagt ttatcgagtc aactaagcgt tatgctgata tcgtcattcc tgaaggggtt
     5761 agcaataccg tggctatcga cctgttgacg accaagattg caaagatttt ggaagaagct
     5821 cgaaacagca aataatcaca tgtggaggcc ttgcctcctt tttctatttt tccctcataa
     5881 tggtacataa atggagattt ttaggcatat ttttggtata ataataccca taaaagagca
     5941 agtaaatgaa tagtaggtgg agatggaaaa gtatttatcg gtaacaactt tgaccaaata
     6001 tctgaaaatg aaattcgata aagacccata cttggaacgg gtctatttaa ctggtcaagt
     6061 ttccaacttt cgtaaacgac ctactcacca atatttctcc ctaaaggatg accatgcagt
     6121 tattcaagcg accatctggt ctgggattta tcagaaatta gggtttgacc tggaagaagg
     6181 aatgaagatc aatgtgattg ggcgtgtaca ggtctatgaa ccaagtggta gctactccat
     6241 catcattgaa aaagctgagc ctgatggggt tggggcgctt gcgattcagt ttgaacaact
     6301 taagaaaaaa ttgacagaag aaggtctgtt tcaagaacgc ttcaagcaag ctctacccca
     6361 attttctaag agaattgggg tggtaaccag ccgtagtgga gccgttattc gagatattat
     6421 cacaaccgtc agcaggcgat tcccaggtgt tgacatcctt ctttatccga ccaaggttca
     6481 aggtgaaggg gctgcggagg aaattgctag aaatattgcg cgtgctaatc aacgggacga
     6541 tttggatttg atcattattg gtcgtggagg tggttctatt gaggatctct gggcctttaa
     6601 cgaagaaatt gtggtacgag ctatttttga atctcgtttg ccagttatct ctagtgtggg
     6661 gcatgagacg gatgtgacct tggcagattt tgtggcagat cgacgcgctg caacgccaac
     6721 agcggcggct gaactggcaa cacctgtgac caagttggat gtattagctc atttgcaaaa
     6781 tcaggaaaaa cggatggcaa cggcagtccg aaatgttcta tctaagaaac aagaggcttt
     6841 gaaaaaatgc agtcagtctg ttatctttag acaacctgag cgcttgtatg acggttattt
     6901 gcaacgcttg gaccaactgc aactgcgttt gaaacaaagt ttgcgaactc ggatttctga
     6961 taacaaacaa ttagttcaag caagaactca tcaattagta caattatcac ctgttaccaa
     7021 aatccaacgc tatcaagacc gtttaggaca gttggacaag ctcttaggta gccaaatggc
     7081 gttagtttat gacgccaagg ttgctgaggc caagcgactt tcggaagctt tgctcatgtt
     7141 ggatactagc cgaatcgtgg cgcgtggtta tgctattgtc aaaaaagaag agtccattgt
     7201 agattcggtt gagagtttga agaaaaaaga ccaagtaacg cttttgatgc gagatggtca
     7261 agtagaatta gaggttaaag atgtcaaaac aaaagaaatt tgaggaaaat ctagcagaac
     7321 tggaaaccat tgtccaaagt ttggaaaatg gtgaaattgc tctggaagat gcgattactg
     7381 cctttcaaaa gggcatggtc ttgtcaaaag agctccaagc tacgctggac aaggctgaaa
     7441 agaccttggt aaaggtcatg caagaagacg gaacagaaag tgattttgaa tgaaaaagca
     7501 agaaaaatta gctcttgtcg agtcggtttt ggaagatttt tatggagacc agcagtttgc
     7561 ctctagttta cgggagtctg ttctctattc tattcatgct ggtggcaagc gtattcggcc
     7621 ttttctcttg ttagaagttc tggaagcctt gcaggttgcc atcaaacctg ctcacgcgca
     7681 ggtagctact gccttggaga tgattcatac agggagcttg attcacgatg accttcctgc
     7741 tatggatgat gacgattatc gaagagggcg gttaaccaat cacaagaaat tcggtgaagc
     7801 tatggccatt ttggctggag atgccttatt cttagaccca tatgccttga ttgcgcaggc
     7861 agatttgcca agtcagatca aggtggactt gattgccagc ttatcccttg cttcaggtag
     7921 tctgggtatg gtggcagggc aagttttgga tatggagggc gaacaccagc acttgtctct
     7981 ggaagaactt cagactattc atgccaataa gactgggaag ttactagcct atcccttcca
     8041 agcggcagct attatagctg aattgtcacc tgaaatgcag gtgaagctga aaactgtggg
     8101 tgaattgatt ggacttgctt ttcaagtcag agatgatgta ctggatgtga cagctagttt
     8161 tgaggaaatc ggtaagacac ctcaaaagga tctgcaggca gaaaaatcaa cctatcctgc
     8221 cttgttgggc ttggaagagt ccattgtctt ttgtaaccaa accctggatc aagctaatga
     8281 aaaattggaa gaaattgccc agcaggttcc ctttgaaaca gaatcgattg taagtgtagt
     8341 agaaagtttg agaatcaatg gctaaggaaa gagtggatgt actagcttat aaacaggggt
     8401 tgtttgaaac gagagagcag gccaagcgag gtgtgatggc tggcctagtc gtagcagtcc
     8461 ttaatggaga acggtttgac aagccaggag agaaaattcc agatgacacc gaattaaaac
     8521 tcaaggggga gaaactcaag tatgtcagcc gtggtggttt gaaactggaa aaggccttgc
     8581 aggtctttga tttgtcggtg gatggcgcga ctacgattga tatcggggcc tctactggag
     8641 gttttaccga tgtcatgcta cagaatagtg ccaagttggt ctttgcagtc gatgttggta
     8701 ccaatcagtt ggcttggaaa ttacgccaag acccacgagt tgtcagcatg gagcaattca
     8761 attttcgcta tgctgaaaag actgatttcg agcaggagcc gagctttgcc agtattgatg
     8821 tgagtttcat ttcccttagt ctgattttgc cagccttgca ccgtgtcttg gctgatcaag
     8881 gtcaggtggt agcacttgtc aaacctcagt ttgaggcagg acgtgagcag attgggaaaa
     8941 atggaattat tcgagatgct aagattcatc agaatgtcct tgaatctgta acagctatgg
     9001 cagtagaggc aggtttttca gtccttggct tggacttttc tcccatccaa ggtggacatg
     9061 gaaatattga atttttagcg tatttgaaaa aagaaaagtc agcaagcaat cagattcttg
     9121 ctgagattaa agaagcagta gagagggcgc atagtcaatt taaaaatgaa taaaaaagaa
     9181 agacttgaaa aaattagaag attggtgaca gattatcaaa tcggcacgca agaagaaatt
     9241 gtagaacatt tgaaagaagc aggtatcact gccactcagg cgacggtatc ccgagatatc
     9301 aaagagttag gtattgtcaa aattcctttg agagacaaca cctatgtcta cgaattgcca
     9361 aaatcaatcg taaaaagtct gcaactggct gaagacaata tcgaatcggc tgaattgatg
     9421 gataagatga tcaatctcca agttattcca ggaaatacgg cttttgtaaa agctcagtta
     9481 atcgagactt ttgcagacaa gatttttagc tgtttgactg atgatagctc gattttagtc
     9541 attgccagaa gtggaagtct agcagaggaa atctttgaac aagtaaaaaa ttggtaggtc
     9601 tatatgttac ttgaaatttc gataaaaaac tttgccatta ttgaggctat ttccctcaat
     9661 tttgaaaagg ggatgactgt cctgactggt gaaacgggtg cagggaagtc gattatcatt
     9721 gatgccatga atatgatgtt gggagctcgt gcgacgacag atgttattcg tcatggtgca
     9781 ccaaaggcag agattgaggg gcttttctca gttgagaata gtcgtctttt acaggaaatt
     9841 tttgatgagc aaggtttaga attaggtgat gaaattatca tccgtcggga aatcttgcaa
     9901 aatggtcgta gtatcagccg tgtaaatggt cagatggtta atctgtctgt tttgcgagct
     9961 attgggcaac atcttgtaga tattcatggt cagcatgacc atgaggagtt aatgcgtccc
    10021 caactgcata tccagatgtt ggatgaattt ggtgatgccg ctttttggga cttgaaagaa
    10081 acctatcaaa cgagttttga tgcctatcgg aaaatgcgca agcaggttct ggaagtcaag
    10141 aaaaaccaac aggaacacaa ggcacgtatc gaaatgttgg aatttcaaat ggcagagatt
    10201 gaggcagcaa acttgcaggc tggagaagac ttggctctca atcaagagcg agataaactc
    10261 ctcaaccaca aaaatattgc ggatacactg accaatgcct acagtatgtt ggacaatgaa
    10321 gatttttcaa gtctggccaa tgttcgttca gctatgaatg acatggaaag tgtcgaagag
    10381 tatgatcctg aataccgtga aatttcaagc tctctgtctg agatctacta tgttttagaa
    10441 gacattagca aacgtttgga agctatcatt gaggaccttg attttgatgg caatcgcctc
    10501 atgcaggttg agaatcgttt ggacctcctt catactatta ctcgtaagta tggtgggact
    10561 gttgatgatg ttttgcttta ttttgccaag attacggaag aatacaatct cttgacaggc
    10621 aataatcttt cgtctgagga catggaagca gagcttaaga agttggaagt caatcttgtc
    10681 aatttggcag gtcaacttgc ttctgctcgt cataatttgg ctcagcaact cgaagctgag
    10741 attaaacaag aactgcaaga tctttatatg gaaaaagccc agtttcaggt tcgttttagt
    10801 aagggaaaat tcagtcgtga gggaaatgaa atggttgagt tttatatttc aaccaaccct
    10861 ggagaagact ttaaaccctt ggttaaggtt gcttctggag gggaattatc tcgtctcatg
    10921 ttagccatta agtctgcctt ttcacgtaaa gaaggcaaga ctagcattgt ctttgatgag
    10981 gtggatacgg gagtttcagg tcgtgttgct caagctattg ctcagaaaat tcataaaatt
    11041 ggtcagcatg gtcaggtttt ggctatctcc catttgccac aagtaattgc gattgcagat
    11101 tatcaattct ttattgagaa gattagtaat gaccattcaa cggtttcgac tgttcgtctc
    11161 ttgacggtcg aagagcgagt ggaggaagtt gccaagatgt tggcaggtga tgatgtgaca
    11221 gaagcagccc tgacgcaagc cagagaattg ttgagaaaca gggagaaata agatgacaga
    11281 ttattatgta attggagatg ttcacggaaa agctgggatg ctggaagacc ttcttaaaac
    11341 ctgggatggt cagacccagt tgctctttct aggtgatttg attgacagag gtgaggatag
    11401 tcaccgtgtt cttgaaatgg tcaaggactt ggtggacaat caaggggcta tctgtttgtc
    11461 tggaaaccac gagtatatgt ttttgacttg gctagatgac ccagaagaaa gttatgacca
    11521 ttatcgtcgc aatggtggag atacaaccat taactctatc ctaggtcgtc ccttggatgc
    11581 accagttgat ggagtagaag atgccaagcg ggttgccgct gaagcggcag acttggtcga
    11641 attcattcgt caaatgccat ttgtagtaga gacagacaag tatatctttg ttcacgcagg
    11701 tattgatttg accttggacg actggcatga aacaacagat tataaaaaag tatggcttag
    11761 aaaaccattc cacggagctg aaaatcatac tggaaaaacc attgtcttct gtcatacacc
    11821 ggtctatggt ttgttacagc aagaacgcgg tacatctgag ctttggacta cagaagatgg
    11881 caagattgga atggatggag gagctgttta tggtggtgtc cttcacggga ttgtctttac
    11941 agaccaagga atgacagaac accactttat cgaaaatgac ggctttgtcg ctgaagatta
    12001 gtactcctag cagggtatgg tcttgtcaaa atatcaaaaa caatttataa taaatagata
    12061 ccctgaaagg aagagaatca tgaacttaga agaattgaaa aaacgacagg agaagatccg
    12121 taacttctct attatcgccc atattgacca cggaaaatca actctagcag accgcatttt
    12181 ggaaaaaaca gagacggttt caagtcgtga aatgcaggcc cagcttttgg atagcatgga
    12241 gctagagcgg gaacgtggaa ttactattaa gttgaatgcc atcgagttga attacactgc
    12301 aaaagatggg gaaacttata ttttccactt gattgacaca ccagggcacg ttgactttac
    12361 ctatgaagtt tcacgttcgc tagctgcctg tgagggtgct attttggtgg tcgatgcggc
    12421 tcaaggaatt gaggctcaaa ctcttgccaa cgtttatctg gctttggata atgatttgga
    12481 aatcatgcct atcattaata aaattgacct gccggctgca gatccggagc gcgtgcgtac
    12541 agagattgaa gatgtgattg gtttggatgc cagtgaagca gttttggctt ctgccaaggc
    12601 tggtattggg attgaagaaa tcctcgagca aattgtagaa aaagtaccag caccaacggg
    12661 tgatgtgacg gcgccactta aggccttgat tttcgactct gtttacgatg cttaccgtgg
    12721 ggttatcctc caagtacgtg tcatggacgg agtggtcaaa cctggtgata agattcagct
    12781 catgagcaat agtaagacct ttgatgtggc cgaagtcggt atttttacac caaaagcggt
    12841 tggtcgtgat ttccttgcga ctggtgatgt tggttacatt gcggcgtcta tcaagacggt
    12901 tcaggatact cgtgtgggtg ataccgttac cttggcaacc aatcctgcgg cagaaccatt
    12961 acatggttat aagcagatga atcctatggt ctttgcgggt ctctacccaa tcgaatcaaa
    13021 caagtacaat gacctgcgtg aagcgcttga aaaattgcaa ctgaatgatg ctagtcttca
    13081 gtttgaacca gaaacatctc aggcacttgg atttggtttc cgttgtggat ttcttggact
    13141 tctccatatg gatgttatcc aggaacgttt agagcgtgag ttcaatattg acctcatcat
    13201 gacagctccg tctgttattt acaaagttaa tttgaccgac ggtgagtcta tggatgtgtc
    13261 taacccatct gagtttccag acccaactaa gattgcgacc attgaagagc cttatgtcaa
    13321 ggcgcaaatc atggtaccac aggagttcgt cggagcagta atggagctag ctcagcgtaa
    13381 gcgtggggac tttgtgacta tggactatat tgatgataac cgtgtcaatg ttatctatca
    13441 aattcctctt gctgaaattg tctttgactt ctttgataaa cttaagtctt caacacgtgg
    13501 ttatgcaagc tttgactacg aattgtcaga atatcgccca tctaagctgg tgaaaatgga
    13561 tatccttctc aatggagata aggtggatgc tctcagcttt atcgttcaca aggactttgc
    13621 ctacgaacgt gggaaactca tcgttgataa actcaagaaa atcatccctc gccaacaatt
    13681 tgaagttcca atccaagcgg ctattggaca caagattgtc gctcgtactg atatcaaggc
    13741 ccttcgtaag aacgtacttg ctaaatgtta tggtggtgac gtttctcgta agcgtaaact
    13801 ccttgaaaaa caaaaagctg gtaagaaacg catgaaatcc atcggatcag ttgaagttcc
    13861 gcaagaagcc ttcctcagcg tcttgagtat ggatgaagaa taagttatca gaaatgcctt
    13921 tccttgttca caggaaattt atataagaat caatagtaaa gcaggcttag atggtctgct
    13981 tttttagttt ttatactctt cgaaaatctc ttcaaaccac gtcatcgtcg ccttaccgta
//
LOCUS       DAVLYV010000045        13679 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLYV010000045 DAVLYV010000000
VERSION     DAVLYV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.045"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..152)
                     /locus_tag="VYO70_001343"
     CDS             complement(<1..152)
                     /locus_tag="VYO70_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713569.1"
                     /translation="MNIPIPFGDILEKEVITNIPKIYQKLKQIYNDLQFKTEEELGVV
                     YQEYLQ"
     gene            complement(193..933)
                     /locus_tag="VYO70_001344"
     CDS             complement(193..933)
                     /locus_tag="VYO70_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1713570.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDETPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(1253..1606)
                     /gene="rsfS"
                     /locus_tag="VYO70_001345"
     CDS             complement(1253..1606)
                     /gene="rsfS"
                     /locus_tag="VYO70_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1713571.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(1619..2119)
                     /locus_tag="VYO70_001346"
     CDS             complement(1619..2119)
                     /locus_tag="VYO70_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1713572.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            complement(2120..2713)
                     /gene="yqeK"
                     /locus_tag="VYO70_001347"
     CDS             complement(2120..2713)
                     /gene="yqeK"
                     /locus_tag="VYO70_001347"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1713573.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEIHTVGAGQMTDLDKVIYVADYIEHNRSFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            complement(2713..3342)
                     /locus_tag="VYO70_001348"
     CDS             complement(2713..3342)
                     /locus_tag="VYO70_001348"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1713574.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRDFIAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(3394..3705)
                     /gene="yhbY"
                     /locus_tag="VYO70_001349"
     CDS             complement(3394..3705)
                     /gene="yhbY"
                     /locus_tag="VYO70_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1713575.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(3919..5025)
                     /gene="yqeH"
                     /locus_tag="VYO70_001350"
     CDS             complement(3919..5025)
                     /gene="yqeH"
                     /locus_tag="VYO70_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1713576.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSNALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(5028..5618)
                     /locus_tag="VYO70_001351"
     CDS             complement(5028..5618)
                     /locus_tag="VYO70_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1713577.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(5570..6478)
                     /locus_tag="VYO70_001352"
     CDS             complement(5570..6478)
                     /locus_tag="VYO70_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1713578.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(6535..7095)
                     /locus_tag="VYO70_001353"
     CDS             complement(6535..7095)
                     /locus_tag="VYO70_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEV1713579.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            7234..8441
                     /gene="sstT"
                     /locus_tag="VYO70_001354"
                     /pseudo
     CDS             7234..8441
                     /gene="sstT"
                     /locus_tag="VYO70_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            8509..9462
                     /locus_tag="VYO70_001355"
     CDS             8509..9462
                     /locus_tag="VYO70_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1713580.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            9605..9985
                     /locus_tag="VYO70_001356"
     CDS             9605..9985
                     /locus_tag="VYO70_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1713581.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            10085..10306
                     /locus_tag="VYO70_001357"
     CDS             10085..10306
                     /locus_tag="VYO70_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1713582.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            10323..10637
                     /gene="trxA"
                     /locus_tag="VYO70_001358"
     CDS             10323..10637
                     /gene="trxA"
                     /locus_tag="VYO70_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1713583.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            10942..11475
                     /gene="queF"
                     /locus_tag="VYO70_001359"
     CDS             10942..11475
                     /gene="queF"
                     /locus_tag="VYO70_001359"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1713584.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWVMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEDLA
                     EQRLFQHDLYPEKIDNR"
     gene            11525..11794
                     /locus_tag="VYO70_001360"
     CDS             11525..11794
                     /locus_tag="VYO70_001360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713585.1"
                     /translation="MLKAVFEVIDKTDEVSSKHCFEVADRTDEVSLKHCFEVVDRTDE
                     VSLKHCFEVADRTDVVSNHTYGKVKLTWFEEIFEEYHTKKPCSSR"
     gene            complement(11819..12487)
                     /locus_tag="VYO70_001361"
     CDS             complement(11819..12487)
                     /locus_tag="VYO70_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1713586.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEK"
     gene            complement(12598..13389)
                     /locus_tag="VYO70_001362"
                     /pseudo
     CDS             complement(12598..13389)
                     /locus_tag="VYO70_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
BASE COUNT         3973 a         3032 c         2618 g         4056 t
ORIGIN      
        1 aattgtaaat attcttgata aacaacacct aactcttctt ctgttttaaa ctgtaagtcg
       61 ttataaattt gctttaactt ttgataaatt ttaggtatat tggtaattac ttctttttca
      121 agaatatcac caaatggtat tggtatattc attttccctt acctcctaag cataagatta
      181 acaatatttg atttacttct gcgccacaaa aaaccaacgg gtgctagttt ctgttggctc
      241 cttatcctca aagtctgcat agagtttgaa ggacttaaat ccagcctgtt ccagcaagat
      301 atcataggtc aagatctcat aggtcctctc ctcatgtact tcatcgtggc gactaaagga
      361 accgtcagcc tccttgataa agaaagtcag ctcatgcacg atggagtgag gagtttcgtc
      421 ctcatatgta tcccaaagca tggcaaaatc ttccgcattt tcatggtagg aatagccagg
      481 gaatacttca tctgtctggt aggtcgagtg cacgtcaaag atgaaaactc cctcttcatt
      541 cagcgcattg tacacgtcct taaagacgtc ccctacttcc acctcatcct gcatatagca
      601 gatagagtcc gaataacacg tgacaaaatc gtatttccct gccttggaca aattcagcat
      661 attaccttct ataaaagcaa tcttttgctt ggctgaagtt gctctcttct ccgcaatctt
      721 caacatatcc gcactcaagt caagtccagt cacatcaaaa ccagcctgag agaagcgcac
      781 tgattgaatt cctgtcccac aagccagttc caagagtttc tttctctcct tggtcttagg
      841 caaatgacgc agagaaaaat tcgtccattt gtcgtataaa ctatcgtcca tgaccgcatc
      901 atagacagcc gcaaaggttt cataagtcgc cataaaacat gataccaagt ccgcccgtaa
      961 ttcgatagaa aattaggagc cttacgatgg agccgagctc caaggaaggc tatctatttt
     1021 ccgataatta caggacgggt tcaaatcctt tctaagatac aaagagcctt aaaagcaaag
     1081 agtacatagg aaactttctt ccctatcgtc agatacaaag aaagttactt tttatctcag
     1141 cttttagctc gtgttcagtt tcgaaatgaa acaaacacga ggccctttac ctttctatca
     1201 atttttctaa aataagcaaa gaaacccagt tgactacaac tgagttcatc ttctaagcaa
     1261 gagcttctga aatatctact gaattcgcct catgccatag cttctctaga ttataatggg
     1321 cacgcatttc ctctgaaaag atatgcacga caacagcacc gaggtccagt aagacccagc
     1381 ctccagctgc atcgccttcg acatggctac ctttaaagcc cgcttgagct actttttcac
     1441 ggatattagc tgcgatagcg tccaactgac ggctattcat tgagctagtg atgacaaagt
     1501 agtccgtcac actagtcaaa tcttgtacat caagtgcaag gatatcctcc gcacgtttct
     1561 catcagccgc tttcacgact agttctagta attctttttc gttcatttaa tcctcttttt
     1621 agaaaatatg tttgtaatct aggacatctg caaagcgctc ctcccaaatg tcctcataaa
     1681 attcattaat cgtttctgct ggaatgtcat ccccatcaaa ggttgtgaca gcaccttctg
     1741 gaacaataag ctgatagcca tattcaaagg caaccttgac agaggtatcc acacaatatt
     1801 ctgtctgcat accacataaa actaattttt caatcccctg tttatccaag tattctttta
     1861 agccagtttc tttgaaaata ctgttatact tcttctgaaa gaccttttca gcaggttttc
     1921 gatttaaaag cgcagataac tgccaatctt ctgatgtttg agcttcagag ttctcaatat
     1981 gttgaacata gataatttca atattcttgc ttctagcctg gttttgtaaa taagaaattt
     2041 tttccaatag actttttgtc tggaacccag tttctactaa aacattctga acatcaatga
     2101 ttataaaagc cgtcttcatt tagtcctctt tcaaatagtg cacaaaggcg ttataggttt
     2161 caagggtttg gggatagatg gggaatccct gatgagctag atactccacg gtacgagctg
     2221 tttcgtaggc caccgcctta ttgagcgata gacttgcaat ctcacgcgcc acatctactc
     2281 caggaaagga tcgattgtgc tcgatatagt ctgcgacgta gatgacctta tctaggtctg
     2341 tcatttgacc agctccgact gtatgaattt caatggctcg cagaatttca gaatcatgca
     2401 aatccaaatc ttcctgaatt ttgtaaattc cgaccatacc atgccagaca ttattgcccc
     2461 agtttttgag gtcagggtct agctggtaac ggtcaatcaa ggctagaaat tcctgatttg
     2521 acaacttttt agcatagtca tgaagaagac ctgctagact tgctttctca acatctacac
     2581 caaagcgctg agctaactcc atagcggcac gctccacacc tagacaatgg gttagacgtt
     2641 tctcaggtag aagctctgcc attttttcca acaaggcctc acgggagcag ttgatatagt
     2701 cttgatagga catcagtaga gcccctcctt ctcgatgtag tctagcactg gctgaggtag
     2761 gagaaagttg ggtttccgac cttgggcaat gaagtcccgc accatgctgg acgagatatc
     2821 catgagcggt acgtccaccc agataactgg ataggaagtt cctgccttgt agcgtggacg
     2881 ctgaaccccc acaaactgaa ccatgtcaac cagttcatca attcggtacc acttaggcag
     2941 atagtcaacc atgtcggcac cgatgataaa gtaataatcc gtatctggat gttgctctgt
     3001 caagattttc atggtgtcgt aggtgtagga aatacccttg cgctccaact caatggtttc
     3061 aatgactagg ccgtcaatcc cctcaattgc caactcaagc atcttgagac gatggtgttc
     3121 agggatggtt tcctttttat caacgtgagg aggttggtat tcaggcatga gaagaacttg
     3181 atccagtccc aactgttgcc gtacttgatc cgcaacaatg agatgggcat tgtgaacagg
     3241 gttaaaattc ccccctaaaa tcccaacttg tttgcgtttt ttctccttga tttctggctc
     3301 caactctacc ttggtaaagg gagtcaatag ttcgattgtc ataggctagt cccctttatt
     3361 tctctataaa aacagttatt tggagtttcg gtcttagatt tctttgactt tcttagaaat
     3421 cttgcgattt tctttcttgc tagattgttt aaacaaaatc aagatgcgtc ctattttttg
     3481 gactgtatcc acaccgattt cttcttccaa aatttcagct acttcgtgga tgttttcatc
     3541 tgtgttttgt aagagagtaa ccttgattaa ttcacgcgca tcaagagctt gacggacgct
     3601 ggttttgatt tggtcgttga gtccattttt cccgatttgg atgataggtt tgagggtgtg
     3661 tgcctggctg ttgaggaagg cacgttgttt tgatgttaat gacataattt tcttccttgt
     3721 ttttggatag ttattttagg tggtgaggga cggtcggaac taattttcca aagatttcat
     3781 ctttgaaaaa tggatttcct gacctcacaa ttgagccttg gtctcaattg tgccccttgc
     3841 caatctacag attggcaatc acaccacggc aataactatc gctatatgag ctcactttgt
     3901 tcgctcagcg attttgtttt aaataattgc ttttcgtgtg acgacggcga cgccttctgg
     3961 tgcccagacg gcgacttttg ctatgcctgt tacacgaatc cagcctaggc ctgagatgac
     4021 taggtctgtc ttatctttaa tggtaaagac atgctggact agccttggga aatcttcttt
     4081 ttccttgcta tttggtggtg tcagaagagt tcccaggtgc ttatcgtaga aagcactagc
     4141 tccttcaagc ttgctacgat ggagtttgag ttcattatca aagaaagcag taaatccttg
     4201 cttttctcct gctataaagt caaagcgtcc caaaccacct aaaaataggg tttgctcagg
     4261 attaagctga taggtcttag gcttgatttc ctttttagga ctgacatact tgaggttttt
     4321 ggccgtcaag tagtgagcca tctggtggcg gtggataatt cccggcgtat cgtaaatata
     4381 agatccgtcg tcaagcggaa tctctatttt gtccaaggtt gtccctggga agcgtgaagt
     4441 agtgatgaca ttctgatcac ccgtgatttc ttggataata gcattgatta gagttgattt
     4501 tccaacgttg gtcacaccga ccacatagac atcgcggccc ttacggtagt gttcaatctt
     4561 gtcaatgact tccttaatgg catgtttatt ttgtgctgaa gttaggacca catcgactgg
     4621 acgaagacct tcttcatggg cacgtttcat gagccactgg ctaatcttac cagacttaac
     4681 tgacttagga aggatatctt ttttatttcc taccaagagg acatcattgc ccgagacgaa
     4741 acgtggtaaa cctgggatga cagatccatt aaaatcaaag atatcaatga cattgaccac
     4801 taaggcatta ctgtctccca cctcgtgcaa gagcttgagg aaatcatcgt tcgtcaactg
     4861 gacatctgtg atttcattgt agtggcggag acggaaacag cgttggcaat agacttcgcc
     4921 agtctccaaa cctttttcaa gtgccgactg gggggtaaaa ccaagaccag ctttatctgt
     4981 cgtctgaatg gttgctccac aaccaataca gagaatttct tccatagtta aattcctttt
     5041 ttatatgtaa tcggtccgta cttttcagtg atttttctca taacacgacg ctcacgagtt
     5101 cggttaatct gcgttttgat tgagtcatgt tggaccaagg gtttgactaa aattgaccga
     5161 atccctgcac ggtgggctgc tcgtatatct gtcatgagct ggtcaccaac catgaccact
     5221 tcctttttgt catagtggaa ttccttcata gcacggtcaa taccaaatgt gaagggcttc
     5281 aaggcccagt aaacgtaatc aatcccaaat ttctcaactg ctcgttgaac gcgttttttg
     5341 gtgttatttg acactacgat aatgccaata cccgcgtccc gaaggtcatg tagccattgc
     5401 ttcatctctg gcgttccatc agggttgttc caagcaatga gggtattatc caaatcgacc
     5461 aaaacagcct tgattccctg cgcctgcagg cttgggactg tcagatcata gactgcttcc
     5521 acagcaaaat ctggtatata attttcaatc gccattctgg ctccttttct taactttttt
     5581 tcgcaatttt ccttagtaac aaggataaaa caatccacaa acctgcacta gccaaactga
     5641 tatagagcca agcatggggc tcatctgtta aaggcaaggg aacattcatt ccgaaaaagc
     5701 ctgtcacgac tgccaaaaca gctagcaaga ctgaaatgat agtcaaggtt gtcaaattgt
     5761 cattcagatt attgtttaga atattgttgt aagaggctga aagctgctgt aaaatctgag
     5821 agattaggtc tgtcatggat accagctgat gagcctcaat catggcatca tcaaactgtt
     5881 ctctctcaat ctcatcaaaa ctacgataca aggcatgacc ttgaatatgc tctaacaaaa
     5941 tccgattttg tttggcagct gccgttagat aaaccatacc agtctccaaa tcagacaggg
     6001 caaagaggtt tttcttggta gttcgctggc gcaagaggtc attgacctca tccctactct
     6061 tgtccatctg ctcaatgaca ggatagtagg cattgctgat gatttccaga ctggcaaaga
     6121 gaaacttata aatcgaaagc gtgtcatggc tctccagata acgagtcatc tgttcaatga
     6181 cataggcgtt cttggtatta ctaatggtaa tcaggcgacg atgctcgaca ataaaggtca
     6241 tgggaaaagt ctcatagtag gccttgtcct tttttacgtc taggacatta tagataaagg
     6301 taaccgtccc actttcacgg tggtagtcca tgtgggcgcg ctcgtttcta tccagtgcgt
     6361 attcaatggt ttccttgtcc aaaccgtaaa tttcagaaag gtcttctagt ttattcaact
     6421 ttcctaggtc taggtctatc caggtacaac cattgcccaa ctgcttttct aaaaccatct
     6481 tttctcctta ttaaactccc tctattgtac cacaaattac ttaaaataac aggtctagtc
     6541 tgtgcgtgag aaattgctaa caactgtgat atttcggtta gggacaaagt gaagggcttg
     6601 ctcctcgcta cgaagctgtg tcgtacgaat tcccacactg acaaccttac ccgatacagt
     6661 aatcggtcca tttgtcagaa cgacctcatc tcccacatcc agttgacgtt caaagaggat
     6721 gaaaaagcca ttgatgacat cagacagaaa gccttgggct cccataccaa tcgctacccc
     6781 agcaatacca gctccagcca gcaaactaga aactggcaaa cctaaaatcg acaaaatgca
     6841 gtagagtaaa aagaaatata gcgtataatt aaacacattt tctagtaaac gtgagatggt
     6901 tttttggcgt ccgacatcat gacgagacat ttttagagaa ggtttgacaa ttctctgcac
     6961 catggtatga agcatttttt tagcaatata aaatacaatc aaaagcagta aaagagaaat
     7021 gaccttggtt agaatattct cgataatggt tgtcacatct agcttttcaa tataagcctg
     7081 aataaatttt tgcatataaa actcctttat ttcattatac catattttca tcagtctcat
     7141 tctccataaa tattcaaaaa atagacagaa aattcttgat tttgtctatc atttatacta
     7201 taatcataat cactacacaa aaaggagatt attatgaaaa gaatcattca tgcttggaat
     7261 aaggcaagcc ttgtcaagcg tatcttgatt ggtatgcttt gtggggaatc ctaggactga
     7321 cccttcctaa tctctcaggg attgggctac tcggggattt attcgttgga ggtctaaaag
     7381 ctgttgctcc tatcctagtc tttgccctcg ttgccaatgc cctttccaac atcaaaaggg
     7441 acaagatagc aatatgaaaa ctgttgtttt cttgtatatc ctatgatagg gactttcgcc
     7501 gctgctcttg tagctgtact agcaagtttc atcgtcccta ttgaaattac cctaaatagt
     7561 gccaatactg aaattgcacc accagatggg gttgggcagg ttctcagcaa cctcttgctc
     7621 aaactggttg acagcccagt caacgccctg tttactgcta actatattag aatcttatct
     7681 tgggcagtca tttttggaat cgctatgaga gaagccagta aaaatagtaa agaattgcta
     7741 aaaactatcg ctgacgtgac ttctaaaatt gtcgaatgga tcatcaatct ggctccattt
     7801 ggaagccttg gtcttgtttt taaaaccatt tctgacaagg gagtcggaag ccttaccaac
     7861 tacggtattt tattggttct attagtaacg actatgcttt ttgttgccct tgtggtcacc
     7921 ctttgattgc cttcttcttt atgagacgca atccttaccc tctagtttgg aactgcctcc
     7981 gtgtcagcgg tgtgacagcc tttttcactc gtagttctgc ggctaacatt cctgtcaaca
     8041 tgaaactctg ccatgacctt ggactcaacc cagataccta ttctgtttct atcccactcg
     8101 gttctactat caatatggct ggagtagcga ttaccattaa cgttttgacc cttgctgcag
     8161 ttaacactct tggaattcct gttgactttg ccacagcctt tgtcctcagt gtggtagcag
     8221 ctatctcagc ctgtggtgct tcaggtattg ccggaggttc cctccttctt atcccagttg
     8281 cttgtagcct tttcggtatt tctaacgata ttgccataca agttgttggg gttggttttg
     8341 tgattggtgt catccaagac tcatgtgaaa cagcccttaa ctcttctaca gatgtcctct
     8401 ttaccgccgt tgccgaatac gcagcaaccc gtaaaaaata actcatcaag gcaagcctgc
     8461 tcatgtcttg tcttttacgc ttttattcta acttattagg aaattcttat gtctattagc
     8521 caacgtacga ccaagctcat cttagctacc tgtcttgcct gcctgcttgc ttattttctc
     8581 aatctttcat cagcagtttc ggctggaatt atcgctctct tgagcctatc tgatacgcgt
     8641 agaagtactt taaaactggc tcgcaatcgt cttttttcta tgcttctagc tctggctatc
     8701 ggtgttctag cttttcactt gagcggattt catatctgga gtctcggcct ctatctggcc
     8761 ttctacgttc ctttagccta caagatgggc tgggaaattg gcatcacacc aagcactgtt
     8821 ttggttagcc atctcttggt tcaagagtca acctctccag accttctagt caatgaattc
     8881 cttctctttg ctattggtac aggatttgcc ttgcttgtta atctctatat gccttcacga
     8941 gaagaggaaa tccagcacta ccacacgctg gtggaagaaa agttaaaaga tatcctccag
     9001 cgcttcaaat actatttatc cagaggagac ggacgcaacc gagcacagct ggtagcagaa
     9061 ttagacacgc ttttgaaaga agccctcaga ctggtctatt tggatcactc tgaccacctc
     9121 tttcaccaga ccgactacca tatccactac tttgagatga gacaacgaca aagtcgtatc
     9181 ctgagaaaca tggcccaaca gattaacact tgtcaccttg ccgccagtga aagcctgatc
     9241 ttagcgcaac tcttttcaaa aattgcaggt caactgagcc agaccaatcc tgcttctgat
     9301 ttgctagatg aaattgaacg ttatctggaa gtcttccgga accgcagtct gcccaagaca
     9361 agagaagaat ttgaaacccg cgccaccctt cttcaactcc tacgtgaagc caaaaccttc
     9421 atccaagtaa aagttgattt ttaccaaaaa tatagacagt aaaaaataaa accactaaaa
     9481 aacagagctt gccagcattc ttgaaaagta gtataattat tgcatgcgca atcatcttgt
     9541 gacgcagaga ctgcccgtta atggactttc ttctatttat gactctaaaa agaaaggaga
     9601 cactatgacc tatttggaaa aatggtttga cttcaatcgg cgtcagaaag aaattgaaag
     9661 tctcttggaa gagaccattg cccaacagag cgagcaaagt ctgaccttga aagagttcta
     9721 cctgctctac tatctggact tggctgaaga aaaatctcta cgccagattg acctgccaga
     9781 taaacttcat ctgagcccga gcgcagtttc taggatggtg gctcgcttgg aagctaaaaa
     9841 ttgcggtcta ctcagtcgca tgtgttgcca tcaagataga cgctctagtt ttatctgcct
     9901 gacaaatgat gggcaaaaga cactggcctc gctacaaaag actgtcgaag aaagcttgga
     9961 agcaggtttg gatttcttga tttaagatga tttttaaaaa aagttgcgtg cgcaatcatt
    10021 tttcttgaca ttctctttta taaggagtaa aataaagtta gcattaaaca aaggagtttt
    10081 aaatatgatt gaaattacct atatagatgc cagcaagaac gaaagaactg tgactttcga
    10141 gtcttatgaa gactttgaac gttcgcaaca agcttgcctt atcggcgtcg cagactacta
    10201 ccctgtccaa aaattaactt acaagggtca taatttggac taccatggga cttatggaga
    10261 tatcttcttc tatctcatga aacaagattt aagccaatat aactaaaaaa aggagaaata
    10321 caatggcaaa agcaattaca gatgcaacat tcgaacaaga aacaaaagat ggtttggtct
    10381 tagtagactt ctgggcaact tggtgtggtc catgtcgtat gcaaggtcca atcttggaca
    10441 aattgtctga agaactttca gaagatgtct tgaaaatcgt taaaatggac gttgatgaaa
    10501 atccaaacac agctcgtgct tttggaatca tgtctattcc aactcttctc ttcaaaaaag
    10561 acggccaagt tgtcaaacaa gttgcaggtg ttcacacagc agaacaaatc aaggccatca
    10621 ttgctgaatt gagctaatca aacgagagac caagtacttt ctttggtctc ttttttcttg
    10681 ccctttgcca tttttcaaaa aatatgctag actatagtag attgaaacta gaatagtaca
    10741 cctctacttc taaaacattg ttagaaatcg atttaactgt cctgatcgat ttgtcctgtt
    10801 cttatttcat tttactatat aagtagaata ttacgatgtt tgggatatgc catcgctaaa
    10861 aaaactcgat ttggtatttt ttagctccct aataggagct ttttgcatgc tctgaacatt
    10921 tcccattttg gaaggagtac tatgaaacgt caatcagcct tggtcgtctt tagtggtggt
    10981 caagattcaa caacctgcct tttctgggtt atgcaacact atgaaacagt cgaagctgtc
    11041 acctttgcct acggccaacg tcatcacctc gaaattcaaa ttgctagaga aatcgctaag
    11101 gaacagggca ttcgtcacca tatcctcgat atgtctctgc tgggacaaat cactgctcag
    11161 ccagactttg cgacgattca tatttcctac attcctgaca agctctgtgt cgagtcaaaa
    11221 tccctcaaac tatatctatt tagctaccga aatcacggag atttccacga aaactgtatc
    11281 aacaccatcg ggaaagactt ggtcaacttg ctagaccctc gctatttaga agtctgggga
    11341 aaattcactc cgcgcggtgg catttcaatc gacccctact acaactacgg taagcaagga
    11401 actaagtatg aggacttggc agaacaacgc ctcttccaac acgaccttta tccagagaaa
    11461 attgacaacc gctaaagtca tactcaatga aaatcaaaga gcaaactagg aagttagccg
    11521 caggttgctc aaagcagtgt ttgaggttat agataagact gacgaagtca gctcaaaaca
    11581 ctgttttgag gttgcagata gaactgacga agtcagctta aaacactgtt ttgaggttgt
    11641 agatagaact gacgaagtca gcttaaaaca ctgttttgag gttgcagata gaactgacgt
    11701 agtcagtaac catacctacg gcaaggtaaa gctgacgtgg tttgaagaga ttttcgaaga
    11761 gtatcatacg aaaaagccct gttcctcaag atgaggagca aggctttttg agtttcaatt
    11821 atttttctgt tccaaggaaa ttttttgcaa caagggctgc aagaactcca ccagcgattg
    11881 gtgcaaggat gaaaatccaa acttgttgaa gggctgcgcc gcctaccaag acagctggtg
    11941 ccaagctacg agctgggttt actgaaagtc cagtaatctt caatccgaca agaatcatcg
    12001 ccatcaatga caaaccgatt accaaaccag caatcgcgcc attgccctta ctttctgaag
    12061 tcacagtcat aataactaag acaaacaaga aagttgcaat gacttcaaac aagaaaccac
    12121 caaagacagt gacaccgttt gccaaggcat tttcaccaag actagcagtt gacatacctg
    12181 aattagccaa gaggaagaag actgcgccag aagcgatgaa agctccaaca acctgaccaa
    12241 ggatgtagtt tacaagttct gaagatgaca aacgtttgtt tacaaacata gcaatcgaaa
    12301 cagccgggtt caagtgagca cctgaaacag ttccgattga gtaggctgcc accacgattg
    12361 ccaaaccaaa ggcaaaggcg attccaaggt gaccaaggcc atcaagacca tttccaaaaa
    12421 caacagctcc tgtcccaacg aacacaagca tgaacgtacc gattaactca gcaacaaatt
    12481 ttttcatttt ctttctcctt ttttcaaaaa ctagatacta gtctatcaaa agtaggaaag
    12541 ggtttcaaga aaattgattg gaaatttttt gaaaatcata gaactattag ctaatctcta
    12601 gtattgaaaa gactggatag cttctttcaa ctcatcttgt aaactatttc tctggtcaag
    12661 ttggacatag acttccacca gacaggatct aaagttggaa aatttgtaaa aatcctccct
    12721 ttcttctatc ggaaaatcaa cagtttttat ccaagaagct acttgttctt gctccaactt
    12781 cccttataaa ataggttcat agatcactct tgctaaacgc caatcctcat catctgtaaa
    12841 gcgaatcgac atccttttaa atagttggcc aagtatatca aatacttcat gaactctgtt
    12901 tttaggaaag tctggatgac aaaccacctc tgtcagtaaa tcggctccat gtgcaaaagc
    12961 gtgaacccaa ccatactgac ttgagaaacc tgttgtatcc ttttcttttg aaagatagtg
    13021 caagccttga tttgaaaggt cattacgaaa tcctgcattt aattcctgat aaaaaaccga
    13081 ttgctggttg gcatcgtcag acaagagagt tgcataaata agcgccctaa aagaacgttc
    13141 aagtgttggc aggcctatct tatcaatctc tttatctaac cctccatcag atgaaacccc
    13201 ttcagcaatg aaatgaaatt gttcctgtgt aaatagctct tcctgaatcc ctctagcaaa
    13261 gcttgtaaaa acaaggtcat cacgaatttc tggagaagga tctcccaaat gatcaagcaa
    13321 ccactggatt tcctcctggt tataatttgg tttttcttct gctatttttc ttagtaaatc
    13381 ttgatacatg gtcaatacct ctacatttct agcaactgtt caaaaaggca gtcttaaatg
    13441 accttaccaa tttgtaaaat taagttaaaa agaaaaagac atttatattc agagaaacag
    13501 catatttctg aaaagatata taaatgattt attccatatt acaacaaata aacttgtaat
    13561 gatagaacct tatcacaaaa atcacattcc tgtctcaaaa attgcgaaaa actagttata
    13621 ataaagtgaa ataaaaacta tacaaattga ttagggaatt taaaacaatt tctaacaat
//
LOCUS       DAVLYV010000046        13543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLYV010000046 DAVLYV010000000
VERSION     DAVLYV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.046"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            49..537
                     /locus_tag="VYO70_001363"
     CDS             49..537
                     /locus_tag="VYO70_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1713587.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            534..1721
                     /locus_tag="VYO70_001364"
     CDS             534..1721
                     /locus_tag="VYO70_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1713588.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAAVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSTKEDNHFKVTVDQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            1714..2079
                     /locus_tag="VYO70_001365"
     CDS             1714..2079
                     /locus_tag="VYO70_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1713589.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            2095..3441
                     /gene="asnS"
                     /locus_tag="VYO70_001366"
     CDS             2095..3441
                     /gene="asnS"
                     /locus_tag="VYO70_001366"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1713590.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            3594..3884
                     /locus_tag="VYO70_001367"
     CDS             3594..3884
                     /locus_tag="VYO70_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1713591.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            3896..4366
                     /gene="ssbA"
                     /locus_tag="VYO70_001368"
     CDS             3896..4366
                     /gene="ssbA"
                     /locus_tag="VYO70_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1713592.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LERRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            4398..4637
                     /locus_tag="VYO70_001369"
     CDS             4398..4637
                     /locus_tag="VYO70_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1713593.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            4932..6332
                     /locus_tag="VYO70_001370"
     CDS             4932..6332
                     /locus_tag="VYO70_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1713594.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            6334..6693
                     /locus_tag="VYO70_001371"
     CDS             6334..6693
                     /locus_tag="VYO70_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1713595.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            6747..7496
                     /locus_tag="VYO70_001372"
     CDS             6747..7496
                     /locus_tag="VYO70_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1713596.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            7486..7785
                     /locus_tag="VYO70_001373"
     CDS             7486..7785
                     /locus_tag="VYO70_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1713597.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            7856..8791
                     /locus_tag="VYO70_001374"
     CDS             7856..8791
                     /locus_tag="VYO70_001374"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1713598.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            8877..9389
                     /locus_tag="VYO70_001375"
     CDS             8877..9389
                     /locus_tag="VYO70_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1713599.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLRDSDF"
     gene            9426..9944
                     /locus_tag="VYO70_001376"
     CDS             9426..9944
                     /locus_tag="VYO70_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1713600.1"
                     /translation="MIRYLDQYEDVILCENKRHYLNFPTLESLDSLELDQEIFVREAS
                     PVYQALLEQSFETELRNQINAAILVEKTDFARIKMTLSNYFYKVKQQYPLTEKQQELY
                     DILGDVNPEYALKYMTAFLLKFLKKDQLMQKCRDIFVDSLVVLGYIVQNEDRKYELAI
                     DFDKERLTFYLA"
     gene            10045..10242
                     /locus_tag="VYO70_001377"
     CDS             10045..10242
                     /locus_tag="VYO70_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1713601.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(10285..11730)
                     /locus_tag="VYO70_001378"
     CDS             complement(10285..11730)
                     /locus_tag="VYO70_001378"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1713602.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVKRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMVIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            11836..13458
                     /locus_tag="VYO70_001379"
     CDS             11836..13458
                     /locus_tag="VYO70_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1713603.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
BASE COUNT         3923 a         2590 c         3074 g         3956 t
ORIGIN      
        1 ttcttatatt cacttgtaat aaagcttaat agatggtaaa atagacgagt gaaaaaaaga
       61 caaaacaaag caaaaaataa tctactgtgg cagtatggtc tagggatgac gattttgttt
      121 gtggttatca gtgcttcctt tctgtatatg gtttctctta gcatgaaacc ctatcaaaca
      181 gctaaaagtg aaggagaaaa attagctcag cagtatgcag gattagagca ggccgatcag
      241 gttgatttat acaatggctt ggaatcttat tacagcgttc ttggtcgtaa taaacagcaa
      301 gaagcacttg ctgttctgat tggaaaagat gatcataaga tttacgttta tcagctaaat
      361 cagggtgttt cacaagaaaa agcagaaacg gtttctaagg aaaagggagc tggcgaaatt
      421 gacaagatta tctttggtcg ttatcaagat aagccaatct gggaagtcaa gtcaggatct
      481 gatttttatc tagtagattt tgaaacagga gcattggtca acaaggaggg cctatgaaat
      541 tatccaaccg tgttttagaa atggaagaaa gtgtgacttt ggctgctgga gcccgtgcca
      601 aagcactgaa ggctgagggc agagatattt tgtctctaac cttgggtgag ccagatttca
      661 ctactcccaa aaatatccaa gatgccgcca ttgcatcgat tcgagatggg cgtgcttctt
      721 tttatacagt aacctcaggt ctgccagagc ttaaggcggc ggtcaatagc tactttgagc
      781 gcttttacgg ctattctgta gcgtcaaatc aagtgacagt cgctgcggga gccaaatatt
      841 ctctctatac cttctttatg gctgcggtca atccaggtga tgaagtcatc atcccaaccc
      901 catactgggt tagctatgga gatcaggtca agatggcaga aggcgttccc gtctttgttt
      961 ctactaagga agacaatcac tttaaggtga ccgtagatca gttagaagca gctcgcactg
     1021 acaagaccaa ggttttggtg ctgaattcgc catctaatcc cacaggtatg atttacaccc
     1081 gtgaggaact cttggcaatt ggaaactggg ctgtagaaaa tgatattctc atcctagcag
     1141 atgatatcta tggccgcttg gtttataatg gtcatgagtt cacaccgatt tctagcctat
     1201 cggaagcgat tcgcaagcaa acagtggtca tcaatggtgt ttctaaaacc tatgccatga
     1261 ctggttggcg gattggttat gccgttggag aagcagacat tatcgctgcc atgtccaaga
     1321 ttgcaggtca aacaacttcg aatccgtcag cagtagccca atatgcagca gttgaggctc
     1381 tatcaggtga gcaagatact gtagaaagca tgcgtcaggc ctttgaggaa cgtcttaata
     1441 ccatctatcc cctccttgca gaggtgccag gatttgaagt ggtcaaaccg caaggggcct
     1501 tctatctctt cccaaatgtc aaaaaggcca tggagatgaa aggctacacg gatgtgacag
     1561 actttacaac tgctatctta gaagaagccg aagtggcctt ggtaacagga gctggatttg
     1621 gagcaccaga aaatgtccgc ctcagctatg cgacagacct agacacgctt aaagaagcag
     1681 tcgaacgctt gaaagcattt atgggtagtg agaatgattg atcattttga gattaaggta
     1741 aaggatttac aaatttcaga aggattttat aggagttttc tcgctccttt ggactataaa
     1801 ttggctttta agactagttc tctaattagt tttctatccc cgaacagccc tcatcctggt
     1861 ggtgattttt ggctgactca gggaacacaa gatcctgttc attttgcttt tttagcagaa
     1921 aataaggaag aagttcaggc ttgttatgag gctggcttag aggcaggtgg gcgagacaat
     1981 ggggctcctg gttatcgaag tgagcatccg atttactatg ctgcttttat gattgacctg
     2041 gatgggaaca atatagaggt ggtttgccat aaagaataaa aaagaaaagg aaaaataatg
     2101 acaaaacgtg taacgattat tgacgtaaaa gactatgttg gtcaggaagt gacgattggc
     2161 gcttgggttg ccaacaaatc aggaaaagga aaaatcgctt tcttacaatt gcgtgatgga
     2221 acagccttct ttcaaggtgt ggcttttaaa ccaaactttg tcgaaaaatt tggtgaagaa
     2281 gtgggacttg agaagtttga tgttatcaaa cgcttgagcc aagaaacgtc tgtttatgtg
     2341 acaggtattg tcaaagagga cgaacgttct aaatttggct atgagttgga catcacagac
     2401 atcgaagtga tcggtgaatc tcaagactac ccaatcacac caaaagaaca cggaacagac
     2461 tttttgatgg ataaccgtca cttgtggcta cgctctcgta agcaagtagc tgtgttgcaa
     2521 atccgtaacg ctattatcta tgcaacttat gagttctttg acaagaacgg ttttatgaag
     2581 tttgacagcc caattctttc aggaaatgcg gcagaagatt ctacagaact ctttgaaact
     2641 gactacttcg gaacgccagc ctacttgagc caatcaggtc agctttacct agaagcaggg
     2701 gctatggctc ttggtcgtgt ctttgacttt ggtccagttt tccgtgctga aaaatcaaaa
     2761 acacgccgtc acttgactga attctggatg atggatgctg agtactcata cttgacacat
     2821 gatgagtcgc ttgacttgca agaagcttat gtgaaagctc ttctacaagg tgttcttgac
     2881 cgcgcgcctc aagccttgga aaccttggaa cgtgatacag aactcttgaa acgctacatt
     2941 gcagagccat tcaaacgtat cacttacgat caagccattg acctcttgca agagcatgaa
     3001 aatgatgaag atgctgacta cgagcatctt gagcatggtg atgactttgg gtcaccacac
     3061 gaaacttgga tttcaaacca ctttggtgtg ccaacatttg tcatgaacta tccagcagcc
     3121 atcaaggcct tctacatgaa accagttcct ggaaatccag agcgcgtgct ttgtgcagac
     3181 ttgcttgctc cagaaggcta tggagaaatt atcggtgggt ctatgcgtga ggaagattac
     3241 gatgcccttg tcgctaagat ggatgaactt ggcatggatc gtacagaata tgaattctac
     3301 cttgaccttc gtaaatacgg tacagttcca cacggaggat ttggtatcgg tatcgaacgt
     3361 atggtaacct tcgcagcagg aacaaaacat atccgtgaag ctattccatt cccacgtatg
     3421 ttgcaccgta tcaaaccata agaacaggaa aacgcccatg tggcgttttt tcgaattcta
     3481 gtgtttttac ttgcgtaaaa aaaatttttc tagtataata gtttattgtg agcgaacctc
     3541 acttacccct tgcaaagtct tggggtcatt agaccaaaag gaggaacata tcaatggcta
     3601 aatacgaaat tctttatatc attcgtccaa acattgaaga agaagctaaa aacgctttgg
     3661 tagcacgttt tgactctatt ttgactgaca acggtgcaac tgttgttgaa tcaaaaactt
     3721 gggaaaaacg tcgtcttgca tacgaaatcc aagatttccg tgaaggactt taccacatcg
     3781 ttaacgttga agcaaatgac gatgcagctc ttaaagagtt tgaccgtctt tcaaaaatca
     3841 acgctgacat tcttcgtcac atgatcgtca aaattgacgc gtaagaaggt aaactatgat
     3901 taacaatgtt gtacttgtag ggcgtatgac acgtgacgct gagttgcgtt ataccccatc
     3961 aaatgtagca gttgcgactt ttactcttgc agtaaaccgt acatttaaga gtcaaaatgg
     4021 tgaacgtgag gctgatttta tcaatgtcgt tatgtggcgc caacaggctg aaaatcttgc
     4081 taactgggct aaaaaaggct cacttatcgg ggtgacaggt cgtatccaga ctcgtagtta
     4141 cgataaccag caaggacaac gtgtctacgt gacagaggtc gtggctgaga atttccaaat
     4201 gttggaaaga cgtagtgtgc gtgagggtca cacaggtgga gcttactctg caccaactgc
     4261 aaactattca gcacctacaa attcagtacc agacttttca cgtaatgaaa atccatttgg
     4321 agcaacaaac ccattggata tttcagatga tgatttacca ttctaatgga caactaaact
     4381 tataaaggag aaaaaacatg gctcaacaac gtcgtggcgg attcaaacgc cgtaaaaaag
     4441 ttgattacat cgcagcaaac aaaattgaat atgttgatta caaagatact gagcttctta
     4501 gccgtttcgt ttcagaacgt gggaaaatcc ttcctcgtcg tgtaacagga acttcagcta
     4561 aaaaccaacg taaagtaaca acagctatca aacgcgctcg cgtaatggct ttgatgcctt
     4621 tcgtaaacga agattaaaga aaagacccag ttgggtctct ttttcatgag cttgaaaaca
     4681 agaaagcgaa ttaagacccc aacgggtctt ttttcacaag ctttgaaatg agagagcgag
     4741 ttaggctcag tggctccctt tttacgagtt tgaaaacaag agaacacatt aaaagcacga
     4801 tggagctttt tcataaatat gtctcttttc tcatccaaga aattaaaata aaaatgataa
     4861 actttttgaa aataggggat ttgcctactt tatgatataa tggaaagagt tagattgaaa
     4921 gaggtagtga gatggacgcg aaattaagat acaaggcaaa gaagatcaag attgtctttt
     4981 ttgatattga tgatacattg cggaattcaa agacagggtt tattccaact acaattccca
     5041 ctgtttttaa acagttgcgt gaaaaaggaa ttttaacagg aatcgcctct ggacgtggca
     5101 tttttggtgt tgttccagag attcgtgatc tcaagcctga cttttttgta actttgaatg
     5161 gggcttatat cgaagataaa aaaggtcagg tcatttatca gcatcagatt gaaaagtcag
     5221 atgttgagga gtatatctct tgggctaagc aagaaggaat tgagtatggc ttggttggga
     5281 gtcatgatgc caagttgtcg actcgcaccg atatgatgag tgaagctatc aatccaattt
     5341 atcccgactt agatgtagat cccgatttcc atgaaaaaga agatatctat cagatgtgga
     5401 cttttgaaga taagggagat gacttgcact tgcctgacag tctctcagac aaacttcgca
     5461 tggttcgttg gcatcaacat tcgtctgata ttgtgccgat ttcaggctcc aaagcgacgg
     5521 gggtggaaaa ggttgtggaa caccttggct tgaaaccaga gaaggtcatg gtttttggag
     5581 atggtctcaa tgacttggaa ctctttgatt atgctggaat cagcgttgcc atgggaattt
     5641 ctcatgataa aatcaaagaa aaagcagatt atattacaaa aacattagaa gaagatggca
     5701 tttttgatgc cttagaagta tttggtatgg tagaaaaaga attgcatttt ccacaagtag
     5761 acattgaaac agtagaaggt cctcttgcga ctattaagac caatcacgga gacttacgta
     5821 tcaagctttt ccctgaacat gctcctaaaa cagtggctaa ctttgtatct ctttcaaaag
     5881 atggctacta tgatggtgtc attttccacc gtattatcaa ggactttatg atccaaggtg
     5941 gagacccaac tggaactggt atgggtggcg agtcaatcta cggcgaatct tttgaggatg
     6001 aattctcaga agagctttac aatatccgtg gtgctctttc catggcaaat gctggtccaa
     6061 ataccaacgg cagccagttc tttatcgtgc aaaaccaaca cctaccttat tctaagaaag
     6121 aaattactcg tggtggttgg ccagaaccga ttgcagaaat ctatgccaat caaggtggga
     6181 cacctcacct agaccgccgt cacacggttt ttggtcagtt agctgatgaa gcatcttacg
     6241 ctgtcttgga tgtcattgct gctgttgaga caggagctat ggacaagcca gttgaagatg
     6301 ttgtaattga aactattgaa atcgaggact aagatgaaaa tcggtgataa gctaaagggg
     6361 cgtattacag ggattcagcc ctacggtgcc tttgttgagt tagagacggg tgatacgggg
     6421 ctgattcaca tttcagagat tcggacagga tttattgaaa atattcatga aaccttgaaa
     6481 gtcgatgaag aggttcaagt tcaggtagtg gatttagatg aatttacagg gaaagccagt
     6541 ctttctatcc gcactttgga ggaagaaaag taccagtttc ctagacggag acgcttttca
     6601 agcgaccgct ttaactacgg ttttgctccc tttcgacgaa tgctaccaat ctggactgga
     6661 gaagccctgc accatttaaa aaagaagaag tagtttagag ttttatattt tttgtaatgt
     6721 taatataaat ttgctataat aggaccatgg aagaagaaca attattaaaa tcaggagagc
     6781 gcattaacca gctcttttcg acagatatca aaatcattca aaatagagag gtttttagct
     6841 attcggtgga tagtgttctc ttatcacgat ttccacgttt tcctaagaag gggttgattg
     6901 tggatttctg tgctgggaat ggagcagtgg ggctttttgc tagcactcgt actcaagcac
     6961 agatattgtc tgttgagatt caggagcgtt tggcggatat ggctgaacgc tctgtccgtt
     7021 tgaatggatt agaggagcaa atgcaggtca tctgcgatga tttgaaaaat atgcctgctc
     7081 acattcaggg gagtaaggtg gatatgattt tgtgtaatcc accctatttc aaggtggatc
     7141 cttattctaa tctgaacgag agtgaacatt atctcttggc tcgacacgaa atcacgacta
     7201 atttggaaga aatctgtcat agtgcccaga gtattctcaa gtctaatggg cgtttggcca
     7261 tggttcatcg tcctgatcga cttctggata ttttggatac gttaaaacga cataatctag
     7321 cccctaagcg cctgcagttt gtttatccaa aaagagaaaa ggaagccaat atgcttttga
     7381 ttgaggcgat caaggatggc tcaacaagtg gctttaaggt cttacctcct ttgattgtcc
     7441 acaatgatga tggctcttat acgcccgaaa tcgaagagat ttattatgga tcataaggct
     7501 tatatgtacg ttctggagtg tcgtgatgga tcctactata taggctatac gactgatatg
     7561 agaagacgcc tcgctatcca caatagtggg aagggagcca aatatacacg agcacgcttg
     7621 ccagtcaaac ttatctatgc tcaaggtttt gccagtaagg aagaagccat gtcggctgaa
     7681 gctcttctca agcgtaagaa gaggccacag aaggaagaat ttttatctga aaatcaagat
     7741 agaaatttac tcagttattt tgaagaatcg tggggagtcc tttgactcct cttacctttg
     7801 tcaaaaagcg aaaaaaatgc tacaataaag aaaataaaca aaatgaggta ttatcatgtc
     7861 caagattcta gtatttggtc accaaaatcc agactcagat gccatcggat catctgtagc
     7921 ttttgcctac cttgcaaaag aagcttacgg tttggatacg gaagctgttg cccttggaac
     7981 tccaaatgaa gaaacagcct ttgtcttgaa ctattttggt gtggaagcac cacgtgttat
     8041 cacttctgcc aaagcagagg gggcagagca agttatcttg actgaccaca atgaattcca
     8101 acaatctgta tcagatatcg ctgaagtaga agtttacggt gttgtagacc accaccgtgt
     8161 ggctaacttt gaaactgcaa gcccacttta catgcgtttg gagccagttg gatcagcgtc
     8221 ttcaatcgtt taccgtatgt tcaaagaaca tggtgtagct gtgcctaaag agattgcagg
     8281 tttgatgctt tcaggtttga tttcagatac ccttcttttg aaatcaccaa caacacaccc
     8341 aacagataaa atcattgctc ctgaattggc tgaattggct ggtgtgaact tggaagaata
     8401 tggtttggca atgttgaaag ctggtaccaa cttggctagc aaatctgctg aagaattgat
     8461 tgacatcgat gctaagactt ttgaactcaa cggaaataat gtccgtgttg cccaagtgaa
     8521 cacagttgac atcgctgaag ttttggaacg ccaagcagaa attgaagctg caatgcaagc
     8581 tgccaacgaa tcaaacggct actctgactt tgtcttgatg attacagata tcgtcaactc
     8641 aaactcagaa atcttggctc ttggtgccaa tatggacaag gtcgaagcgg ctttcaactt
     8701 caaacttgaa aacaatcatg ccttccttgc tggtgccgtt tcacgtaaga aacaagtggt
     8761 acctcaattg actgaaagct ttaatgcgta agattttggg tgtcagctca aaatcggaaa
     8821 gtctagtttg ccttatatcg aaaggagttt cggctccttt tttctaggag tgaagtatgt
     8881 tagaaaatgg cgatttgatt tttgtgagag atgggtcaga catgggacag gccatccaga
     8941 cttccacagg taactatagc catgttgcca tttatttgga tgggatgatt tatcatgcta
     9001 gtggacaggc tggtgttgtc tgtcaagaac cggcagactt ctttgagtcc aatcatttat
     9061 acgacctcta tgtttaccca gaaatggata tccagtcggt gaaggaaaga gcttgcaaac
     9121 atcttggagc accctacaat gcttctttct atccagatgc agctggtttc tactgctccc
     9181 agtatatagc agaaatccta cctatttttg aaactattcc tatgaaattt ggagatgggg
     9241 aacaggagat tagtgatttt tggagggagt attacataga actaggtctg cctgttcctc
     9301 tgaaccaagc tgggaccaat cctagtcagt tggcagcatc gcctctgtta caatgtaaag
     9361 aaaggaatct tcgtgattca gatttttaat ccatctcgtt tgacgagaca gccatttttg
     9421 gagaattgat ccgctatctg gaccagtatg aggatgtgat tctatgtgaa aataagcgcc
     9481 attaccttaa ttttcctacg cttgaatcac ttgatagtct tgaactggat caagagattt
     9541 ttgtcagaga agctagtccg gtctatcaag ccttgttgga gcagagtttt gagacggaat
     9601 tgcgcaatca aatcaatgca gctattttag ttgaaaagac ggactttgcg cgcattaaaa
     9661 tgaccctgtc caattatttt tacaaggtca aacagcagta tcctttgaca gaaaaacagc
     9721 aggagctcta tgacatttta ggagatgtta atcctgagta tgccctcaag tatatgacgg
     9781 cttttttgtt gaaatttctc aaaaaagacc agcttatgca gaaatgccgt gatatctttg
     9841 tggacagttt ggttgtctta ggctatattg tgcaaaatga agatagaaag tatgagttgg
     9901 ctatcgattt tgataaggag agattaactt tctacttagc gtgatttctt gtttctgaga
     9961 acattgtttg actttcctta gtattcggta taaactatat gtaaccggta acacatatcg
    10021 gaataaacta aaggagacaa tcatatgtca cttgaaaaca aattggaaca agcaacaggt
    10081 gctgtcaaag aaggttttgg taaagttact ggagacagca agacagaact tgaaggagct
    10141 gttgaaaaaa cagttgctaa ggcaaaagac gttgtagaag acgcaaaagg tgctgtagaa
    10201 ggtgccgttg aaggtttgaa aaacgttttt actaaagaat aggaaaaaat caagggtttc
    10261 attttccctt gattttttct attcttataa ataattttct gcgacggctg tatctcctgg
    10321 gtaggattct ttcttgccct ggatgatttg gtaacaatcg gctcccttac ccgcaataat
    10381 aactgcatct aattcgtgat ttgtgatagc cattgccgcc ttgatggctt cttggcgatc
    10441 cgcaatcttt tcaacaggat gattgatgta gctactaatt tcatctgcaa tgaccattgg
    10501 gtcttcatag ttagggtcat cagcagtcag aaagacttga atctcagggt gttgattgag
    10561 gaggaggcca aagtccttac gacgactttc tcccttgttt cctgttgatc ccagaaccag
    10621 agcaatcttt ccggtttgat gagtttcaac cacattgatg agttttttca gactatcccc
    10681 attgtgggca tagtcgatga agaccttggc tccatttttc tgagtgagga cttccatacg
    10741 accaggaacg cgggttgcag cgatgccttt tttgatgtcc tcaagacttg ctccgagacg
    10801 gagacaagca agtccagcag caactgcatt ttcttggttg aagttgccaa tgagttgaat
    10861 atcataatct ccagcgagtt tacccgtagc tgaaaagcta aaggctttgg aattctcgat
    10921 ttggttatca aattggctac catagaaatc atggtcttga tcttcaacct gttctttcaa
    10981 gactgagaag tggtccatgt cactgttaat gatgactgct cggctatttt ccatcaagag
    11041 acgcttgtgg tagaaatagt cttcaaagct agggtgttca atcgggccga tatggtctgg
    11101 gctgatattt aggaaaactc ccacatcaaa ggttagacca tagacacgtt tgaccagata
    11161 ggcttgactg gagacttcca tgatgaggtg ggtacggtca ttttgcacag cctgattcat
    11221 catgtcaaag aggtcaatac tctcaggggt tgtcaacgct gacttaaaga aagtctcgcc
    11281 atcaagagtt gtgttcatgg tcgacaacat agcaggtcta tgcccttgag ataagatgtt
    11341 ataggcgaaa taggctgctg ttgtcttacc cttagtacca gtaaaggcaa ggagtttgag
    11401 tttttcctgt ggattaccat agaactccat ggcaatcaaa ctcatggctt tctttatatc
    11461 gttcacaatg atgacaggga taccgacttc gtagtccttt tcagctacat accaagctaa
    11521 tccttgtgtt atagcagaaa gaaggtattc ttttttaaag gcagcgcctt ttgcgaaaaa
    11581 aagagtgtct tctgttactt ttcggctgtc gtagctgatg ctatcaaaaa taactttgct
    11641 gtagttgtag tggtaatgac cttggtcaat aatttcgcga aaaaggccat ctttctttaa
    11701 aatatctaat acggtttcaa tcttaatcat actttctatt gtaaaccgaa agtcgtaaat
    11761 ttacaagtaa caaggaaaag tttataatgg aagataagga gtttttccta gctatcaaaa
    11821 ttgaatgagg aatctatgtc gcacgaaaac aatcaccagc aggcccagat gttacggggg
    11881 actgcttggc taacggctag taactttatc agtcgcctac tcggggctgt ttacattatc
    11941 ccttggtaca tctggatggg ggcttatgca gctaaggcaa atggtctctt taccatgggt
    12001 tacaatatct atgcttggtt cttgttggtt tcaacagcgg ggattccagt tgcggtggcc
    12061 aagcaagttg ccaagtataa taccatgcga gaagaagagc atagctttgc cctgattcgg
    12121 agcttcttag gctttatgac aggactaggc ctggtttttg ctttagtctt gtatgtcttt
    12181 gctccttggc tagcagactt gtctggcgtg ggcaaagact tgatcccaat catgcaaagc
    12241 ttggcttggg gagtcttgat tttcccgtct atgagtgtta tccgaggatt tttccaaggg
    12301 atgaataacc tcaaacccta tgccatgagc caaattgctg agcaggtcat tcgtgttatc
    12361 tggatgctcc tagcaacctt tatcattatg aagctcggtt caggagatta tctagcagcc
    12421 gttacccaat caacctttgc tgcctttgtc ggtatggtag ccagttttgc agtcttgatt
    12481 tatttccttg cccaagaagg ttcactcaaa agagtctttg aaacaggaga taagattaac
    12541 agtaagcgtc tcttggttga taccattaag gaagccattc cttttatcct gacagggtct
    12601 gccatccagc tcttccagat tttggatcag ctgaccttta tcaatagtat gagctggttt
    12661 accaactaca gcaatgagga cttggttgtc atgttttctt atttctcagc caatcctaat
    12721 aaaatcacga tgattttgat ttctgtaggg gtttcgattg ggagtgttgg tttgccactt
    12781 ttgacggaaa actatgtcaa gggggacttg aaagcagctt ctcgtctggt tcaggacagt
    12841 ctcaccctac tctttatgtt cttgctacca gcaacggttg gagtggttat ggtaggagaa
    12901 cctctatata cagtcttcta tggtaagcca gatagtttgg ctctgggctt atttgtcttt
    12961 gcagttttgc agtctattat tttaggcttg tacatggtct tgtctccaat gcttcaggcc
    13021 atgttccgca accgaaaggc cgttctctat tttatctatg gttctattgc caagctagtc
    13081 ttgcaactac ctaccatcgc cctcttccac agttatggtc ctttgatttc aacaaccatt
    13141 gctctcatca ttcctaacgt cttgatgtat cgggatattt gtaaagtaac tggtgtcaag
    13201 cgcaaggtga ttttgaagcg aaccatttta atcagtttgc tgaccctagt catgtttctg
    13261 ttaataggaa ccatccagtg gctgttagga tttttcttcc aaccaagtgg acgtttgtgg
    13321 agcttctttt atgtagctct tgtcggtgcc atggggggtg gactttatat ggttatgagt
    13381 ctgcgtacct atttgttaga taaggtaata ggaaaagccc aagcagatcg cctgcgagca
    13441 aaatttaagc tttcgtaagg tagcatttat aaataaaagg aataagttga acaatctttt
    13501 aggagaactt gaaacttatt ccttttttag cgtaaaaata gct
//
LOCUS       DAVLYV010000047        13254 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLYV010000047 DAVLYV010000000
VERSION     DAVLYV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.047"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..1149
                     /locus_tag="VYO70_001380"
                     /pseudo
     CDS             1..1149
                     /locus_tag="VYO70_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(1233..2435)
                     /gene="rpsA"
                     /locus_tag="VYO70_001381"
     CDS             complement(1233..2435)
                     /gene="rpsA"
                     /locus_tag="VYO70_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1713604.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2597..2668)
                     /locus_tag="VYO70_001382"
     tRNA            complement(2597..2668)
                     /locus_tag="VYO70_001382"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2634..2636),aa:Gln,seq:ttg)
     gene            complement(2678..2758)
                     /locus_tag="VYO70_001383"
     tRNA            complement(2678..2758)
                     /locus_tag="VYO70_001383"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2722..2724),aa:Tyr,seq:gta)
     gene            complement(2808..3038)
                     /locus_tag="VYO70_001384"
     CDS             complement(2808..3038)
                     /locus_tag="VYO70_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1713605.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(3206..3850)
                     /gene="pcp"
                     /locus_tag="VYO70_001385"
     CDS             complement(3206..3850)
                     /gene="pcp"
                     /locus_tag="VYO70_001385"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1713606.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3865..4788)
                     /locus_tag="VYO70_001386"
     CDS             complement(3865..4788)
                     /locus_tag="VYO70_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1713607.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4785..5474)
                     /locus_tag="VYO70_001387"
     CDS             complement(4785..5474)
                     /locus_tag="VYO70_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1713608.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5539..6243)
                     /locus_tag="VYO70_001388"
     CDS             complement(5539..6243)
                     /locus_tag="VYO70_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1713609.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKCGNVAPSQKDYDLNSTGWAPSYQDPASYLNIIDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDILTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(6299..7321)
                     /locus_tag="VYO70_001389"
     CDS             complement(6299..7321)
                     /locus_tag="VYO70_001389"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1713610.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGIGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7453..9924)
                     /gene="parC"
                     /locus_tag="VYO70_001390"
     CDS             complement(7453..9924)
                     /gene="parC"
                     /locus_tag="VYO70_001390"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1713611.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAATRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(10344..12287)
                     /gene="parE"
                     /locus_tag="VYO70_001391"
     CDS             complement(10344..12287)
                     /gene="parE"
                     /locus_tag="VYO70_001391"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1713612.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVVNTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            12423..13064
                     /gene="plsY"
                     /locus_tag="VYO70_001392"
     CDS             12423..13064
                     /gene="plsY"
                     /locus_tag="VYO70_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1713613.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(13098..>13254)
                     /locus_tag="VYO70_001393"
     CDS             complement(13098..>13254)
                     /locus_tag="VYO70_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1713614.1"
                     /translation="YSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSQA"
BASE COUNT         3837 a         2979 c         2334 g         4104 t
ORIGIN      
        1 aaccttctaa aattccttat cttgaaacga ctggtatgcc tactagaatt ctccttagaa
       61 agcgtcgatt caagtgctat cactgttcaa aaatgatggt cgctgaaact tctatcgtca
      121 agaagaatca ccaaatccct cgtatcatca accaaaagat tgctcaaaag ttaattgaaa
      181 agatttctat gactgatatt gcccatcagc tttccatctc aacttcaact gttattcgta
      241 agctcaatga ttttcacttt aaacatgatt tttcttgtcc tcctgagatt atgtcctggg
      301 acgttgaaac agtccgggga gtgactgttt caatcgggag atggagatga gcttcattgc
      361 gcaagatttt aacaatctca atatcatcac tgttcttgag ggtagaacac aagctatcat
      421 ccgagatcac tttcttaaat atgatagagc cgtccgatgt cgcgtcaaaa tcattactat
      481 ggatatgttt agcccttact atgacttggc taaacagctt cgctttcaaa tttctaggct
      541 caggctgaaa cagtctccca gactgtttca ctcccgaatg ctaaaatcgt tcttgatcgc
      601 tttcacattg tacaacatct tagccgtgct atgagtcgtg tgcgtgtcca aatcatgaat
      661 caattggatc gaaaatccca tgaatacaag gctatcaagc gctactggaa actcattcaa
      721 caggatagcc gtaaactgag tgataagcga ttttatcgtc ctacttttcg tatgcactta
      781 accaataaag agattttaga caagcttttg agctattcac aaaacttgaa acaccactat
      841 cagctcttgc tttttcactt ccagaataag gaaccagaga aacttttcgg acttattgag
      901 gacaatctga agcaggttca tcctattttt cagactgtct ttaaaacctt cttcaaagat
      961 aaggaaaaga ttatcaacgc ccttcaacta cactattcta atgccaaact ggaagcgacc
     1021 aataatctca tcaaacttat caagcgcaat gcctttggtt ttcgaaactt tgaaaatttc
     1081 aaaaaacgga tttttatcgc tttgaacatc aaaaaagaaa ggacgaaatt tgtcctttct
     1141 cgagcttagc ttttttcaac ccactacagt tgacaaagag ccgaaaaatc ccttgtttag
     1201 taaacaaagg attttgtgaa ttttcaattt gattaaagtt cgatatcacc aaacaaatca
     1261 gccattgaaa atcctgtttg tgtttctgga agttcgaaat cacgcttttc ttgacgtctt
     1321 ggacgacgtg gacgagcagc acgtttttct tctttttgtc cttcttcttg ggctggacgt
     1381 tcttcaagag ctttaataga aagtgatacg cgttctgcat ctgcgttaac ttcaagaact
     1441 ttaacttgaa cttcttgacc aactttaaga gcttcttttg gattttcaat ccgtttgtgt
     1501 gaaatttgtg atacgtgaac aagtccatcg atacctggca atacttcaac aaatgcaccg
     1561 aagtcagtca aacgtttaac tgttccttct actacatcac ctttagccaa tttttgctca
     1621 acgccatccc atggtcctgg tactgttgct ttaagtgaaa gtgatacacg tccttcttct
     1681 tcgttaagat caaggatttt cacttcaatt tcttcaccaa cagttacaac tgattttggt
     1741 gatacgttac gttcatgtga caattcagtc aagtgaacca atccgtcaac accaccaagg
     1801 tcgatgaaag cgccgaagct tgtgatacga gcaactttac cagttacaac atcaccaaca
     1861 gccaatttac cgaatacttc agcgcgagct gctgcagtag ctgcttcaac aacttcacga
     1921 cgtgaaagga tgaagcggtt ttctttagcg ttaacttctt tgattttagt atcaaattct
     1981 tgacctacaa aacgctcagc gttacgtacg aaacgagtat ccaacattga agctgggata
     2041 aatccacgaa caccttcaaa ttctactgaa agtccacctt taacggcacg cgttccttta
     2101 acagtaacaa cttcttcttc gcgaccaaca agtttgtccc atgctttgcg agcttcaagg
     2161 cgttttttag atacaaggta tgtaactgta tcagtatctt taccaactac ttgacgaagt
     2221 acaagaacat ccaatacttc tcctacttta acaaagtcat tgatatctgc atcacgatcg
     2281 tttgtcaatt cgcgaagagt caagacacct tcaacaccag ttccagagat tgcaacgtta
     2341 gcttgagtcg catcaactgt caatacttca gcactaacaa catcaccagt ctcaacttga
     2401 ctaacgctat ttagcaaatc ttcaaattcg ttcatctaaa aaatcctcca acaatcaagt
     2461 ttttcttaaa cttgacatac ttataatttt tttcctaagc acccgcaagg acatgttcat
     2521 atcattcacg tctttcaatt ataaaaataa aataccctaa gatattttgc tttttatctt
     2581 gattattacc taagaactgg ggtagctgga ttcgaaccaa cgcatgaggg agtcaaagtc
     2641 ccttgcctta ccgcttggct ataccccatt aatgaaatgg agagagaggg attcgaaccc
     2701 ccgaacccga aggagcggat ttacagtccg ccgcgtttag cctcttcgct atctctccta
     2761 caatcaacat ggactattat atcatgaaaa tttcaaaaaa acaagactta ttttgctaaa
     2821 ttataatttt caatcaaaat ttccacagct ttttccaatt ttttataaaa actatcgaca
     2881 ttcccattct ttataatggc aaattgtccg tctaattcgg caatttctcc gataactttt
     2941 ttaccgatag tcaatgtata accttcaaaa ctgtctttac caacattaac tttggcatcc
     3001 gctacttgaa tttcgatttt tttatctttc ttgctcattt catacctctc ttcacttttt
     3061 ctattttaca agaaaatcct aaaactagca agaaaaaact ctatatcagg cttctctgat
     3121 atagagtttt atgtaaacaa cctcaatcga tttagcatag ctaatagtta gtaaacaagc
     3181 ataattgact atcctttcta ggttatcagt gagtctcacc gcctactaac tgcaaatcct
     3241 tatctttgta gtctacaata gcttcaatag ctacctctat gcctcttaca atatcacata
     3301 gactcattcc tgcagtattc ggcttattta ctacttgttc catcatatat ggaatatgca
     3361 tgaaacctgc tctcatatta gggaattttt tatccactaa ataaagagct tggtacatca
     3421 aatgattgca aacaaaggtt cctgcactat tggatacaac tgccggaagt ccctgttttt
     3481 tgatagcttg taccatcgct ttgataggta aactactaaa ataggccgat gctccatcaa
     3541 tacgaatcgg tgtatcaatt ggttgattgc cttcgttatc aggtatgcga gcatcatctt
     3601 gattaatagc cactcgttca ggtgttaagc cggtcctgcc gcctgcttgt ccaatacaaa
     3661 gtacagcatc tggttgatat cgtaatattt ctgcctctaa aacttctgac gacttataaa
     3721 aaaccgttgg aatttctacc cagcgaactt cagccccatt aatctcagat ggtaataatt
     3781 ttacagcctc caaagctgga ttaatctttt cacctccaaa aggattaaaa cctgtaacca
     3841 atattttcat tttattttcc tttactaaaa tgcgagaaag tacattaaga atatgtgaat
     3901 aacaatcatt actagagcaa cacctgcttg agcctttata acgccattct gatctttcat
     3961 atccatcaat gctgctggta gagcgttaaa attagcagcc attggggtca ataaggtccc
     4021 acaataacct gctgtcatgg caagagcacc agccacaatt ggattagctc ccagagcaaa
     4081 tacaaaggga actccaacac ctgctgtaat aacggtgaat gctgcaaaag catttcccat
     4141 aatcattgtg aatagaacca ttccaagaac ataggccaaa actcctataa agcgactatc
     4201 tgaaggaaca ataccgctaa tcagatgaga gataacatca ccaacacctg ctacagtaaa
     4261 aatagccccc aaagccccta ataattgagg aacaatacca cttgttgaaa cttgctgagt
     4321 cattcgatta ttttctgata acagactctt agggtgacta ttggtaatca caagaacaga
     4381 aattgtagca aacaaggcgg caaggctaat cgaaatcttg ctaaattctg gaatcatttg
     4441 cgctaagacc aacgcaagta ttgccatcag cataactgga ataaaaattt tatttttcaa
     4501 cctgttagat tcaatattgg ctttcatttc atctaaggat ggcaaggttc cgatacggac
     4561 ttgcttaaac aatgttaaca gcgataatag gattacaata ataccaatac tcatatttgg
     4621 catataggaa ccacctataa acgtaataga caatagagtc caaaatgcag atgtcccaag
     4681 tcgaactggg tttgttttat ctttataact acaataggct gtatggagaa attgacaacc
     4741 aatcacaata taggtcaact ctaatagttg ctttgccaac tctgtcattt ttgttctcct
     4801 cccctagtct tttttgatat caatttttta tcaaataaat aattgtaaat ccccactaca
     4861 ataagtgtta taacagcaac aataatagat gtagaagcaa tccctgcata attgctttca
     4921 tagcctaact gatctaatgt tccccctatc aagaggactc ccccagcacc tacaaacgta
     4981 ttttgagcaa agaaatttcc aaaattttca ttcgcagccg cacgcgcttt tattgtctca
     5041 tcttcaacct ctgttaactt tctacctaat tgagactctg cagctgcttc tcccataggt
     5101 tgaaccaaag gtctgacaaa ctgagggtgt cctcctagac gaattgaaaa gaaaccagct
     5161 aactctcgaa taaagaaata aactgtatag aagtttccaa ctgtcagacc tttaatcttt
     5221 cgaatcaaat cgattgatcg ttgcttgagt ccaaaggttt ctgacagccc cacaagaggc
     5281 aaggtaacca taaaaatcgt gagcactcgc tgattgctaa attcttttcc caaaatctcc
     5341 aaaaattcaa cgagagaaac acctgaaact aaagctgtaa ccaaaccagc taagactact
     5401 gttgcaattg tatcaaattt taaaataaaa cccacaacaa tgattgctat tcctattaat
     5461 ctaatccact ccatatcaaa ctcctttata ttcaaaatga cagtattttt aaaattttat
     5521 caagatcaat accattcctt atttaatgtg tttttctagt tctttttggt atttgctatt
     5581 ggattccaat ttttcttttt gccatttttt aaaaacctcg ttatattctt ttgttgtaag
     5641 aatatctttt tgcaatttca ttcctttaaa gatatatgga tcccccttaa taccaacttg
     5701 tgagtatggt tttgagaatg gtactacgtt acttacaact ggagaaccac cagatgaagc
     5761 tgttggcatc aataatgaac tatctgtcga ccaagcttga gctttggcat atttttcata
     5821 tcttttctct aggtcagtgg tctcagaaac agcatcttct aacaatttct tatatttatc
     5881 caaaccaggt ttagctacaa catccttatc ttttcctttc gtaataccaa ggtgtttcat
     5941 ggcagaacca gattttggat ctataatatt caagtaagac gctggatctt gatagcttgg
     6001 agcccatcct gtactattca aatcatagtc tttttgagaa ggagcaacat tgccgcattt
     6061 atcattttcc atcaaaccat caataacatt tccaataacg tctgtcgtcg atgttcgagt
     6121 cgctatactg tagcccaatg atgctggatc tactgcatag acataagaaa atgttgtcgg
     6181 tgcatctgct tctttatcag tttttccaca agccactaaa atagctgacg tgctcaggac
     6241 cactcctgct gttaagagcc actttttcta tttcataaaa aatctccttt ggtttatttt
     6301 aatctacttt tacaatccaa ccttctggcg cttcaatatc gccaaactga atacccgtca
     6361 attcattata taatttacgc gtcacaggac ctacttctgt ttcactatag aatacatgga
     6421 aatcatcacc atgttgaata cctccaattg gagaaataac cgctgctgta ccacaggcac
     6481 ctgcctctac aaaacggtca agattatcaa ttggaacatc accctcaata ggagttaatc
     6541 ccaagcgatg ttctgccaaa taaagcaagg aatacttggt aatagatggc aagatagatg
     6601 gactcaatgg tgttacaaat tcattatcag ctgtaattcc aaagaagtta gctgatccga
     6661 cttcttcaat ctttgtatga gttgatgggt ccagatagat aacatctgag aaatgacgtg
     6721 acttggccat ttttcctggt aagagacttg cagcatagtt tccaccaacc ttagccgcac
     6781 ctgtaccatt tggtgctgca cggtcgtact catcctgaat caagaagttg gttgggacca
     6841 aaccaccttt aaagtaattt ccaactggca tagcaaagat ggtgaaaatg tattcttctg
     6901 ccggttttac cccgataata tctccgacac caatcaaaag agggcgaaga tataaggttc
     6961 cacctattcc gtatggtggt acgtattctt cattcgcacg gacaactgct ttacaagctt
     7021 ctacaaacat gtctgtcgga acttgtggca tcaagagacg gtcacatgta cgttgcaggc
     7081 gtttagcatt ttcatcagga cggaacagtt gaacactgcc atccttagta cgataagctt
     7141 tcaaaccttc aaatgcttgt tgtccatagt gaagacttgg agaagactct gaaatatgca
     7201 aagttgcatc ctctgtaagc tctccttgat cccattgtcc atttttgaaa tgagcaagat
     7261 agcgataagg taatttcata taggaaaaac cgaggttttc ccaatcaatc gttactgtca
     7321 tattccactc cttattctaa aaacctattt cttatattct acactatttt tctaaaatag
     7381 caagtatatt ttgtaatttt cagaaaattt ctccaataaa aaccagctct tagaactgat
     7441 tcttcatttc acttatttat cttcagtaac tacttcctga agataagcgt caaaaacttc
     7501 ttcatctgaa atcgtgtcag aaatgaagct tccattgcta gtgcgttctg acaagttcaa
     7561 gtcttgcaat cggctttcat agattgttcc tttattggat tggacaagca gagtttggtc
     7621 gttcacatcc acttccgtac tgaagaaatc gccaacaaat ccttgctctg caactgctcc
     7681 tgccaagaag acacgatgcg gtttgttttt caactcacgc aagacttgta atcctcgttt
     7741 ggcacggctg gttgctagaa tttcctcaat ggaaacacgt ttcaagcttc cacgctgggt
     7801 caagaggtag aaggacgaag tattacagat aaagccagat tggaggacat catcttcttt
     7861 caaattcata gccttgacac ctgctgcctt agcaccgaca accggaacct cttcgatatt
     7921 gaaacgcagg gcataaccat tttgactaac caagacaaca tcatctagtt taatcggagc
     7981 cactgctaca atctgatctg tatcgtcttt gagcttagca tacttgacag acttagatct
     8041 ataggtccgc catggagtaa attcttttcg ctctacccgt ttgatttgac caaggcgagt
     8101 cgctgcaaag taggttgtcg catcgtcaaa ctgatccagt acttccacat aaagaatttc
     8161 ttcattcgtt tcaaagtttg tgatggtttg gctcagatgc tctccgatgt ccttccaacg
     8221 aatatctgcc aactcatgga ttggtctgta gatgacattt ccaagacttg tgaacatcaa
     8281 gaggtgctgg gttgtcttgg cagattgaac aaaaatcaaa cggtcatcat cacgcttgcc
     8341 aatttcttcc aaggtggaag ccgcaaagga acgtggactg gtacgcttga tgtaacctgc
     8401 cttggtcacg ctgacgtagg tatcttcctc agcgataaga ctagctgtat caatctcaat
     8461 tgctttcgca gtatcttcta aagaactcaa acgaggagtt gcaaatttct tcttgacctc
     8521 acgaagttct ttcttcatga gattgtacat agtcctttca tcaccgataa tagccgccag
     8581 catagcaatc ttctcacgaa gctctgcttc ttcttcctgc aagacaacca catcggtatt
     8641 ggtcaaacgg tacagttgca aagttacgat agcctcagcc tgttcttccg taaaatcata
     8701 gctaactttg aggttttcct tggcgtccgc cttattctca gaagcacgga taagagcaat
     8761 gacttcatcc aaaatcgaaa tcacacgaat caaaccttcg acgatatgga gacgtttctc
     8821 agccttttct ttgtcaaagc gtgaacgcgc caaaatcact tctcgacggt gagcgatgta
     8881 gctagacagg attggaacaa tcccaacctg acgaggtgtg aaattgtcaa tcgccaccat
     8941 attaaagttg tagttgattt gtaggtcggt gtacttaaat aagtagttga gaacaagctc
     9001 agtattagcg tctttcttaa gttcgatagc gatacgaaga ccatcacggt cagactcatc
     9061 acgaacctca gcaatcccag ctaccttgtt attaacacga acatcatcga ttttcttgac
     9121 tagattggcc ttattgattt cataaggaat ctcagtaata acgatttgtt ccttaccacc
     9181 ttttagcttt tcaatttcag tcttggaacg aacaaccacg cgccctttcc cagtctcata
     9241 agctttcttg atttcatcac gaccctgaat aatagcccct gtagggaagt ctggtccagg
     9301 caagaattcc atgagtttat caatctttgc agttgggtgg tcaatcatgt aaactgcagc
     9361 atctatgacc tcagctaaat tatggggagg aatgtctgtg gcataaccag ccgaaatccc
     9421 agtcgaacca ttgaccaaga ggtttggaaa ggctgctggc aagaccgttg gttctttctc
     9481 cgtatcgtca aagttccatg caaaaggaac tgtatttttc tcgatatcct gaagaaggta
     9541 gcctgcaatt tcagacaaac gtgcctcagt ataacgcata gccgcaggag gatctccgtc
     9601 catagaaccg ttattaccgt gcatttcaac tagaatctca cgatttttcc agttctgtga
     9661 catacgaacc atggcatcat agatagaaga atccccgtgt gggtggaaat tccccatgat
     9721 gttcccgact gacttggccg acttacggta gctcttgtca aaagtattgc tatccttatt
     9781 catagaataa agaatacggc gctgaaccgg cttcaaccca tcacgaatat ctggcaaagc
     9841 ccggtcttga ataatgtact tggagtagcg accaaagcgc tctcccatga tgtcctccag
     9901 ggacatgttt tgaatgttag acataagata caaagcccat aaaataccaa gtgaaaatag
     9961 aaaattcttg aagtaagcaa actcacaaga gaatttatct ttttcacaca gtatctaggg
    10021 cgtgttcaac tcctttcaaa gaatgtagag taggttttta tgcagtaaaa gatattttac
    10081 gggaattcgt cccgtgttca gttacgataa gtaaccaaac tatcctgttt gtatttttca
    10141 atatgaaaat ctggttttcc aaaattagtc ttagtttgtg tcttagccgc tcccttaaac
    10201 gcctctttga gataagcact catagaagat tcttcattaa taatcctgca attttttcaa
    10261 accaagattt tcaaactgct tttttacata gtcattcaca tccgactcta atttccagtt
    10321 tactaacata ttattttctt tcattaaaac actgtcgctt cttctagcgt aaacttgaca
    10381 ttatcttcaa tccatttacg gcgtggttct accttatctc ccatgagaac attgacgcgg
    10441 cgttcggcgc gcgctaaatc ttcaattgtg acacggatga gggtacgtgt ttctgggttc
    10501 atggttgttt cccagagctg gtccgcattc atctcaccaa gtcctttgta tcgttggagg
    10561 gtagcgcctt taccgaactg tttacggagt tcttctagtt ctccgtccgt ccaagcgtag
    10621 gccacttctt ctttcttgcc tttacctttg gacatcttgt aaagaggtgg gagggcaata
    10681 tagacatgac ctgcctcgac tagcggacgc atgtaacggt agaaaaatgt caagagcaag
    10741 gtctggatat gggcaccgtc ggtatccgca tcggtcatga taatgatctt atcatagttg
    10801 gcatcttcaa tagagaagtc tgctccaaca cccgcaccaa tggtataaat catggtattg
    10861 atctcttcat ttttgaggat atccgccatc ttggccttgg ctgtattgac aaccttacca
    10921 cgaagaggta gaatagcctg gaacttgcgg tcacgacctt gtttggcaga accaccggca
    10981 gagtccccct caactagata gagttcattc ttagcaggat tcttagattg ggctggggtc
    11041 aatttcccag acaacaagcc cttatctttc ttgtttttct tcccatttcg gctctcatca
    11101 cgcgccttac gtgctgcttc acgagcatca cgggccttga tagccttgcg gatgaggtta
    11161 gaagctaatt ccccattttc cataaggaaa aaggtcaact tatcagccac tattccatcc
    11221 acaactgggc gagctagggg gcttcctagt ttatccttgg tctgtccttc aaactgcaag
    11281 tgttcttcag gaactaagat agaaagaacg gccgctagtc cctcacgata gtctgaacct
    11341 tcaaggtttt tatctttttc cttgagaaga cctgttttac gtgcatagtc attcatgacc
    11401 ttggtaatgg cagacttgag tcctgtctcg tgcgttccac cgtccttggt gcgaacgtta
    11461 ttgacaaagg atagaatgtt atctgagaat ccgtcattgt actggagggc tacttccact
    11521 tgaaaaccat tgtcttcccc ttcaaagtaa agaactggcg tcaagatttc cttatcttcg
    11581 ttgagataag aaacaaaatc ttgtactcca ttctcatagt ggaactcaat cgcttcatct
    11641 gttcgcttgt ccgttaaaga caaggtcaca tttttcaaga gaaaggctga ttcattaagg
    11701 cgctctgaaa tggtattgta cttgaaatct gtcgtagaaa agatagtcgc gtcaggcata
    11761 aaagtaactt tggtgcctgt tttagacttg ggtgctgtac cgattttctt caaagtcgtg
    11821 acaggttttc caccattttc gaaacgttgc ttgtaaactg cgccatcacg ggtaatttca
    11881 acttctaacc agctagaaag ggcgttaaca acggaagaac ccactccgtg aagtccacct
    11941 gatgtcttat agccaccttg accgaatttc cctccggcat gaagaatggt aaagataacc
    12001 tcaacagttg gaattcccat agcgtgcata cctgtcggca tcccacgtcc atggtcttga
    12061 accgttagac taccgtcttt attgatagtt acatcgatac gatcaccaaa cccagacaag
    12121 gcttcatcga ctgcattatc aacgatttcc caaactaggt gatgaagacc agcgccatcg
    12181 gtcgatccaa tatacatccc cggacgtttt cggaccgcat ccaacccttc tagcacctga
    12241 atagcatcat cattataatt gttaatattg atttcctttt ttgacacaag gaacctccta
    12301 ttcgttcatc tttactattc tacaggtttt ccaaggattt tgcaaaattt ttctttctcc
    12361 gatgtgacaa tttcagcaga gattctctgc ttttctttcc caattcatga tataatagga
    12421 gtatgattac aatagtttta ttaatcctag cctatctgct gggttcgatt ccatctggtc
    12481 tctggattgg acaagtattc tttcaaatca atctacgcga gcatggttct ggtaacactg
    12541 gaacgaccaa taccttccgc attttaggta agaaagctgg tatggcaacc tttgtgattg
    12601 actttttcaa aggaacccta gcaacgctgc ttccgattat ttttcatcta caaggcgttt
    12661 ctcctctcat ctttggactt ttggctgtta tcggccatac cttccctatc tttgcaggat
    12721 ttaaaggtgg taaggctgtc gcaaccagtg ctggagtgat tttcggattt gcgcctatct
    12781 tctgtctcta ccttgcgatt atcttctttg gagctctcta tcttggcagt atgatttcac
    12841 tgtctagtgt cacagcatcg atcgcggctg ttatcggggt tctgctcttt ccactttttg
    12901 gttttatcct gagtaactat gactctctct tcatcgctat tatcttagca cttgctagtt
    12961 tgattatcat tcgtcataag gacaatatag ctcgtatcaa aaataaaact gaaaatttgg
    13021 tcccttgggg attgaaccta actcatcaag atcctaaaaa ataggctctt tgtcaactgt
    13081 agtgggttga aaaaaagcta agcttgagaa aggacaaatt ttgtcctttc ttttttgata
    13141 ttcagagcga taaaaatccg ttttttgaag ttttcaaagt ttcgaaaacc aaaggcattg
    13201 cgtttgataa gtttgatgag attattggtc gcttccaatt tggcgtttga atag
//
LOCUS       DAVLYV010000048        13142 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLYV010000048 DAVLYV010000000
VERSION     DAVLYV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.048"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..85)
                     /locus_tag="VYO70_001394"
     CDS             complement(<1..85)
                     /locus_tag="VYO70_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1713615.1"
                     /translation="MKLNYDDKVQIYELRKQGYSLEKLSNKF"
     gene            363..458
                     /locus_tag="VYO70_001395"
                     /pseudo
     CDS             363..458
                     /locus_tag="VYO70_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(711..1331)
                     /locus_tag="VYO70_001396"
     CDS             complement(711..1331)
                     /locus_tag="VYO70_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1713616.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1331..1813)
                     /locus_tag="VYO70_001397"
     CDS             complement(1331..1813)
                     /locus_tag="VYO70_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1713617.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDQMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1813..2655)
                     /locus_tag="VYO70_001398"
     CDS             complement(1813..2655)
                     /locus_tag="VYO70_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1713618.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2750..3550)
                     /locus_tag="VYO70_001399"
     CDS             complement(2750..3550)
                     /locus_tag="VYO70_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1713619.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     TYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3694..5073
                     /gene="glmU"
                     /locus_tag="VYO70_001400"
     CDS             3694..5073
                     /gene="glmU"
                     /locus_tag="VYO70_001400"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1713620.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAEQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            5083..5628
                     /locus_tag="VYO70_001401"
     CDS             5083..5628
                     /locus_tag="VYO70_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1713621.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5640..5954
                     /locus_tag="VYO70_001402"
     CDS             5640..5954
                     /locus_tag="VYO70_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1713622.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5971..6663
                     /locus_tag="VYO70_001403"
     CDS             5971..6663
                     /locus_tag="VYO70_001403"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1713623.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            6740..7231
                     /gene="rocS"
                     /locus_tag="VYO70_001404"
     CDS             6740..7231
                     /gene="rocS"
                     /locus_tag="VYO70_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1713624.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7329..7919
                     /locus_tag="VYO70_001405"
     CDS             7329..7919
                     /locus_tag="VYO70_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1713625.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLNLSQLFD"
     gene            7928..9691
                     /locus_tag="VYO70_001406"
     CDS             7928..9691
                     /locus_tag="VYO70_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1713626.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYISVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPSLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
     gene            9905..10541
                     /locus_tag="VYO70_001407"
                     /pseudo
     CDS             9905..10541
                     /locus_tag="VYO70_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            10714..10893
                     /locus_tag="VYO70_001408"
     CDS             10714..10893
                     /locus_tag="VYO70_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713627.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAP"
     gene            10939..11355
                     /locus_tag="VYO70_001409"
     CDS             10939..11355
                     /locus_tag="VYO70_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713628.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGMEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            11586..12293
                     /gene="ccdA2"
                     /locus_tag="VYO70_001410"
     CDS             11586..12293
                     /gene="ccdA2"
                     /locus_tag="VYO70_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1713629.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            12304..12861
                     /gene="sdbB"
                     /locus_tag="VYO70_001411"
     CDS             12304..12861
                     /gene="sdbB"
                     /locus_tag="VYO70_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1713630.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGQEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
BASE COUNT         4117 a         2298 c         2757 g         3970 t
ORIGIN      
        1 caaatttatt tgaaagcttc tctaagctat atccttgttt tctaagttca tagatctgaa
       61 ctttatcatc ataattcaat ttcataataa aaacacccca aaagttagat tttttctgtc
      121 taacttttgg ggtgtagttc atgtacacct gatatgatgc gttttataat tttaaagact
      181 ttttgaccag cctcattttt taacttgata ctcagtgaaa agcaaagatt aaactaggaa
      241 gctagctgta ggctgctcaa agaacagctt tgaggttgca gataaaactt gtgaggtaac
      301 caacatatat aatgtgaagc tgacgtggtt tgaatagatt ttaggagggt atgagtctgg
      361 aagttttaat ggataatgca agattccata gaatgggtaa gctagagttc ttatgtgaag
      421 agtttgggca taaactttta ccttttcctc cctactcatc ttagtataga aaagtgaatc
      481 tgaaatagta cataactgct tctaaaacat tcttataaat tgatttaaat tctcaaatca
      541 tattattcag ttcttatttc attttgctct acaatcctgt tgagaagaca cgtgttcata
      601 tcaaaaaggt attggcaagt tgcaatacct ttttacaagg ttcttttgtc ttatttttgt
      661 ttcaactgac tatatctcct atggttctag ttcagaaggc taggctataa ttatgattga
      721 taagaagtat cattccaagt attgagagtg aatgtttcaa aatcatgggt ttctataatg
      781 gtcaggctgg catttgctag accgccatct ttacgaagaa gtggttcttt atagcctagg
      841 agagtacgaa gactggcagt aagattggcg ccgtgtccga caattagaat acgctcagct
      901 ggactatctt ttaatgattt gataaattgg atggtccgct gagttgtact atagagggat
      961 tcggctccga acattcgagt gtcaaattga gcaagatttg aacgaaaagc ctggatttgt
     1021 tgcgggtaaa tagcttccag ggttgcaatt ttcaaacctt ctaacttccc aagttgccat
     1081 tcacggagat taggaacgat ttctaaagaa cagggggtat ggagttgact ttggataatc
     1141 tcagcagatt tgaccgctcg aggtaaatca cttgaataaa tctgatcaaa aggaatttcc
     1201 ttgagatact ggccaagtcg ttttagggtt tcaatggatt caggaagaag aggagaatca
     1261 ccactagcac cttgaaaacg accttcttgg ttccagaggg tacgaccgtg gcggacaaag
     1321 tagagtttca ttacttgatg tcctccaaaa tatctacaaa gtctgccttt acaaagctag
     1381 ccaagtcttg tggcgcgacg ataatgctgt gtccgacttc gcctgcagag acaatcattt
     1441 gatccaaatc tagagcaatt ttatcgataa aaatgggata attgtgtttc tgacgaattc
     1501 cgacaggatt attggctcca tgaatgtaac cagttgtttt ttctaagtcc ttttgtggaa
     1561 tcatgctcac ttttttattg ccagaaattt tagctagttt cttttcagac aagtgctgag
     1621 tgatagggac aattccgata atcggtccgg tcttgtctcc caaaagcgcc aaggttttga
     1681 aaatctgatc tcgttcataa ccttgaggaa gctctccttc tagggcattg atttgaatcc
     1741 cctgatgagg gatagctgct ttagatagga tttgttccac caatgttttt ttgattttaa
     1801 ctttttttgc cattatttat atttatcctc caattgactc atccaaatac caagccagat
     1861 tcccagcgca aagaagaagg cgatgatgac ataaccgaca agtgaaagtc ctgtgtattg
     1921 gatactttca gcgtttcctg catttggaat taagatcaaa agggtacttg ataggacgat
     1981 accgatgatg aaatgataga cacgtgagtg gtagttgttt aaggcataat ccatcaattt
     2041 tgaaaaaacg atgagagttg cacctgcacc aattccaatc ggaaagaagg ttcccaagaa
     2101 atcaaaagtt ttaaaaccag tcaacatagg agcatagagt cccaaaatca aaagtaaatt
     2161 tgatgggctg aggccaggaa ccaagacgcc aagggccaat agtgcgcctg ctaggacgaa
     2221 gttaagaaag ctggcgctta aggttccaac gacaaaattt aaggcataga gtcctaatcc
     2281 agaaatgata aaggttgtcc ataaccaagc taaatcaatc ttgtctcggt cagattctcg
     2341 agttgattct ttgaggaggc taggaactgt accgataata gctcccgcaa agctccataa
     2401 tacaaaaacc tgataatttt caagcaggta ttcaatcggg taggaaaata agccgattcc
     2461 cagaagcata ccgatggcaa ctggaataaa gtacaaaaca ttttctttaa agtctttaaa
     2521 gggatgggcc agaaagccaa tcattcgttc atagattcct aagattgctg ctagaacccc
     2581 tccggaaatt cccggtagga taaatccaag agcaattaca atccctttaa taacgcgtgc
     2641 taaccatgag agcatatatt tcctccaact atttctattt caaaaaatcc ttactatatt
     2701 gtatcacaat cagacacaaa aagaaaaagc aaatgataaa caaatgcttt taaggtttta
     2761 aaaaaagttt tcgaaaggtt tcttctttat tttttaaggg agagataacg ttgatatcta
     2821 aatcgtggtc aaagccggca atttttcctt tagatgtgta ttggtgaata tcataatcta
     2881 aatcagtttt aggactgctc tccaaaaatc ctgagtctga gccgtaggac ggaatccaaa
     2941 cagaggtaaa cttgcctgta tcaatactgt gttcttccat gaagtagaca ccaacgtaga
     3001 tgccgatgtt tttagcacct agtgatgcta gttttgctcg aaagttttcg acaccttcgt
     3061 tcatattaga catggttttg tcttccacgt caagccaata gtaactaggg ctgtaaggag
     3121 aggcagcatt gtagaaaact tcggcagcct tttccatttc ttggacactt tttccagcta
     3181 cataagcgta gacagcaact gggacattcc gcttttgaag ttcagtgata tgactcttat
     3241 aggtcttgtc tattccattg ataaatgaag catcattttc ttttgttgtt tgagcaccac
     3301 tgtgaacacg aacaatagca cctgaaatat tttgtgagag ggcatcgtag ttgatttcct
     3361 caggacgctg ccagccagag aggtcaataa tcggtttgtc taagtgtttc aaagcctgtg
     3421 cttcaatctg tgctatattg gattttgttt taaacgattg gctgtcatta agtgggcgat
     3481 tgatgattaa aatgaacatc ataatcccaa aaaaactaaa taaaataagt ggatgaattt
     3541 gttttctcat atcttataat tctaccctaa aaatcaaaaa aaatcaaaaa aatgggttaa
     3601 ggaagagact ttagagcatt ttttcattca agagtgcgga atgatttgaa atatggtata
     3661 ataaaaggga atttctacag aaaagagaag attatgtcaa attttgccat tattttagca
     3721 gcgggtaaag ggactcgcat gaaatctgat ttgccaaaag ttttgcacaa ggttgcgggt
     3781 atttctatgt tggaacatgt tttccgtagt gtgggagcta tccaacctga aaagacagta
     3841 acagttgtag gacacaaggc agaattggtt gaggaggtct tggctgaaca gacagaattt
     3901 gtgactcaat ctgaacagtt gggaactggt catgcagtta tgatgacaga gcctatctta
     3961 gaaggtttgt caggacacac cttggtcatt gcaggagata ctcctttaat cactggtgaa
     4021 agcttgaaaa acttgattga tttccatatc aatcataaaa atgtggccac tatcttgact
     4081 gctgaaacgg ataatccttt tggctatgga cgaattgttc gtaatgacaa tgctgaggtt
     4141 cttcgtattg ttgagcagaa ggatgctaca gattttgaaa agcaaatcaa ggaaatcaac
     4201 actggaacat acgtctttga caacgagcgt ttgtttgagg ctttgaaaaa tatcaatacc
     4261 aataacgctc aaggcgaata ctatattaca gacgtcattg gtattttccg tgaaactggt
     4321 gaaaaagttg gcgcttatac tttgaaagat tttgatgaaa gtcttggggt aaatgaccgt
     4381 gtggcgcttg cgacagctga gtcagttatg cgtcgtcgca tcaatcataa acacatggtc
     4441 aacggtgtta gctttgtcaa tccagaagca acttatatcg atattgatgt tgagattgct
     4501 ccggaagttc aaatcgaagc caatgttatc ttgaaagggc aaacgaaaat tggtgctgag
     4561 actgttttga caaacggtac ttatgtagtg gacagcacta tcggagcagg agcggtcatt
     4621 accaattcta tgattgagga aagtagtgtt gcagacggtg tgacagtcgg tccttatgct
     4681 cacattcgtc caaattcaag tctgggtgcc caagttcata ttggtaactt tgttgaggtg
     4741 aaaggatctt caatcggtga gaataccaag gctggtcatt tgacttatat cggaaactgt
     4801 gaagtgggaa gcaacgttaa tttcggtgct ggaactatta cagtcaacta tgacggcaaa
     4861 aacaaataca agacagtcat tggagacaat gtctttgttg gttcaaattc aaccattatt
     4921 gcaccagtag aacttggtga caattccctc gttggtgctg gttcaactat tactaaagac
     4981 gtgccagcag atgctattgc tattggtcgc ggtcgtcaga tcaataaaga cgaatatgca
     5041 acacgtcttc ctcatcatcc taagaaccag taggagccta tcatggagtt tgaagaaaaa
     5101 acgcttagcc gaaaagaaat ctatcaagga ccaatattta aactggtcca agatcaggtt
     5161 gaattaccag aaggcaaggg aactgcccaa cgggatttga ttttccacaa tggggctgtc
     5221 tgtgttttag cagtaacgga tgaacaaaaa cttatcttgg tcaagcagta ccgcaaagct
     5281 atcgaggcag tctcttacga aattccagcc ggaaaattgg aggtaggaga aaacacagcc
     5341 cctgtcgcag ctgcccttcg tgaattagag gaagaaacag cctatacagg gaaattagaa
     5401 ctcttgtacg atttttattc agctattggc ttttgtaatg agaagttaaa actatattta
     5461 gcaagcgatt tgacaaaagt ggaaaatccg cgtccgcagg atgaggatga aaccttggaa
     5521 gtccttgaag tgagcttaga agaagcgaaa gaattaatcc aatcaggtca tatctgtgat
     5581 gccaagacaa ttatggctgt tcagtattgg gagttgcaga aaaaatagag gaggtcagta
     5641 tgggtaaatc tttattaacg gatgaaatga ttgaaagagc taatagaggc gaaaaaattt
     5701 caggtcctcc tttgctagat gataatgagg agactaagat tttaccaacc tcttcttccc
     5761 gttttggtta tgccaatcct aaggatcatg gttttagcca ggaaaccttg aaaattcagg
     5821 tcgaaccatc tattcataaa agccgtcgca ttgaaaatac caagagaaat gtcttcaatt
     5881 ctaagttgaa taaaatctta ttcgcggtca tctttctctt gattttgctt gttttagcaa
     5941 tgaaactttt gtaatagaaa aggaattgaa atgaaaatag gaattattgc tgctatgcca
     6001 gaagaactgg cttatctggt ccagcattta gataataccc aggagcaagt tgttttgggg
     6061 aatacctatc atacaggaac cattgcttct catgaagtcg ttcttgtaga aagtggaatt
     6121 ggtaaggtca tgtctgctat gagtgtggcg attttggctg atcatttcca ggtggatgcc
     6181 cttattaata cgggttcagc tggggcagta gcagaaggta tcgctgttgg ggatgtcgtg
     6241 attgctgaca aattagccta tcatgacgtg gatgtcacag cttttggcta tgcttatgga
     6301 caaatggcgc aacaaccgct ttatttcgaa tcagacaaaa cctttgttgc tcaaatccaa
     6361 gagagtttat ctcaattgga ccaaaactgg catcttggtt tgattgctac aggagatagt
     6421 tttgttgcag gaaatgacaa gatagaagcg attaagtccc atttcccaga agttttagcc
     6481 gtggagatgg agggggcagc tattgctcaa gcagcgcatg ccctcaatct cccagtctta
     6541 gtcatcagag ctatgagtga caatgccaac catgaagcaa acatcttttt tgatgagttt
     6601 attatcgaag ctggacgtcg ctctgcccaa gtcttgttga cctttttgaa ggctttagat
     6661 taagcggaaa tttgacagtt tttctagctt atgataagat ttaagtaaag aaaagctaga
     6721 aaacgtttca gaggatatta tgagtattga aatgaccgtc agtgagattg cagaggtctt
     6781 aggattatct cgccaagcaa tcaataaccg tgtcaaagaa ttaccagaag aagacacaga
     6841 taaaaatgac aaaggggtaa cagttgttac cagaagtggc ttgattaagc tagaagaaat
     6901 ctataaaaaa acgatttttg aagatgagcc tgtcagtgaa gatgtcaaac aacgtgaact
     6961 gatggagatt ctagtggatg agaagaatgc agaaattctt cgtctttatg aacaattaaa
     7021 agccaaggat cgtcagttat cagaaaaaga cgagcagatg cgtatcaaag accgtcagat
     7081 tgctgagaaa gacaaacaat tagatcagca acaacaattg acccttcaag ctatgaagga
     7141 tcaagaaaat cttaagctag agctggacca agcaaaagaa gaagtccaat ccactaagaa
     7201 aggctttttt gctcgtttat ttggaggata atcaaggagc tgtttaggtt aaatgctaac
     7261 ctgatggctt cttttgtttg tgaatagtat agttgattag aacagagaca gtagaggaga
     7321 ggtacagaat ggaacgatta gaagattaca ttgcttttga tttagaattt aatcagcacg
     7381 aaggacaaac acacttgatt caagtatcgg cagtgcgttt tagagatggt cgggaaatcg
     7441 atgcctatga ttcctatgtt cataccagcg ttcctctaaa aagctttatc aatggtttga
     7501 ccgggattac agctgagacc ttaaaagatg ctcctccagt tgagaaagtc ttagaagaat
     7561 tccaagagtt tgtgggttct ttgctgatga taggctacaa tgctgctaaa agtgatttac
     7621 ctattttagc agagcatggt ttagactata gccaacagta caaggtggat ttgtatgatg
     7681 aagcttttga acgtcggagt tcagatttac atggtattgc caatctcaaa ttgcaaactg
     7741 ttgcctcatt tcttggattt caaggtcagt ctcataatag tttagaggat gctcggatga
     7801 cggcgcgtgt ttacgaatcc ttcttagagt cggatcaagc tagggaattg ttagagacag
     7861 aaagtagctt atcaaacaat ccttttggtg gcttgaactt gtctcaatta tttgactagg
     7921 agtgcctatg aaacctgaaa aacctaaaaa attgggtctt aaacagattt acctcaatct
     7981 tgataaaatc ctctttctat ttttcttgat ttttatgata gtggagtatt tgtgggtacc
     8041 attcaattca tggttggcta gcttcttatt agatcagaca ggttatcttt ttatttccta
     8101 taacaatgtc ttagccatcc ttactcattc tccattgatt agtctagctt ttttagcctt
     8161 gattgtaatt aaccttctgg ttgcttattt tcaaatagga ctcctcttta ttggtgctcg
     8221 tcatctcctc tataatgaaa agcgaaggct aattgagtat atccgtaagg ttttccatga
     8281 aagttttgga tttatgaaaa agctaacttg cgctaaagcc ttgtttatct ttttctatat
     8341 agctatgctt tttcctttta tacggaaaat gctgaagatt tattatctta ataaaatcgt
     8401 cattccagag tttatccaag cttatttaga agataaatat tggatgtggt ggctgagcat
     8461 cctacttcta tctcttatct ttctctatat ctctgtcaga ttgatgtttg cccttccgaa
     8521 gattgtatat gatcagctga ctgttcgaga agcagtgatg tttagcttgg agaaaaccaa
     8581 gaagagagtt actttttatg cttggaattt attttacatc ttgctcaaag cacatttatt
     8641 attttatctt cctttgattc cttcattatt ggctcaaact ttggtagatg atatcactca
     8701 gagagagtct ctgattcttg gtattttgaa ttttgtcgtg attaaaaatc tctattatat
     8761 ggctctgact tattttttgg ttaaatttgt ttcatttttg acaggaaagg agctggatat
     8821 gcttcctagg agagaaaaag atcacatcgt gcgatggggt gtcatgactt gtgccagtct
     8881 tttctttgcc ttagaaggtt atatttatct ggaggctccc atggttcatc tacctcaact
     8941 tatttctcac cgaggggttt ccaatgcaaa tgggattcaa aatacagtag agtccttgga
     9001 aactacagca caactcaaac cagacttggt ggagacggac gtgcaggaaa caaaagatgg
     9061 gcagtttgtc atgatgcatg atgctaactt gaaaaatcta gcaggtatca ataaaagtcc
     9121 tcaagactta aacttggagg agcttaaagg gattgatatt tttgaaaatg gctaccagac
     9181 taaaatttca agctttgaag attatctcag tcgagccaac gaacttggtc aaaaattact
     9241 aattgaaatt aaaaccagta aaaaagatag tccagacatg atgaaccgct ttttagcccg
     9301 ttatgctgca aagctcaaga tttatggaca tcaaatccag tctttagact accatgttgt
     9361 cgaaaaagta agacagtatg atgccgaact gccagtttat ttcatcatgc cctacaattc
     9421 tgtatttcct aaaacaagag cgacaggata tactatggag tactcaacct tggatgaata
     9481 ttttgtaagc aaactatgga caacggatca gaaactttat gtctggactg tcaatgattc
     9541 agaagctatc agcaaatctc ttcacttagg agtagatgga gtgattacag atgatttgga
     9601 aaaagttcag cgggaaatag aagtagccca agaagatcca gaatatacgg atttgctctt
     9661 gaaaaaagca ttggaattct ttgaatttta gtaataaaaa agaggaactg ttgttcctcc
     9721 catgataggt aagtattctt gtgaatgctt gcttttttgt ggttgcaatc cgtttacgag
     9781 aattgtttgt attgttcttg tttcaattta ctttatagtg ctttgaagtt ggaatgctac
     9841 actataactt ctaaaatata gtaaaatgaa ccaaaaatag tacacaatgt ggtataatct
     9901 tcttatggca tattcaatag attttcgtaa aaaagttctc tcttattgtg agcgaacagg
     9961 tagtataaca gaagcgtcac acgttttcca aatctcacgt aataccattt atggctggtt
    10021 aaagctaaaa gagaaaacag gagagctaaa ccaccaagta aaaggaacaa aaccaagaaa
    10081 agttgataga gatagactta aaaactatct tactgataat ccagacgctt atttgactga
    10141 aatagcttct gaatttggct gtcatccaac taccatccac tatgcgctca aagctatggg
    10201 ctacactcga aaaaaagaac cacacctact atgaacaaga cccagaaaaa gtagccttat
    10261 ttcttaagaa ttttaatagt ttaaagcacc tagcacctgt ttagattgat gaaacaggat
    10321 tcgatactta tttttatcga gaatatggtc gctcattaaa aggtcagtta agaagaggca
    10381 aagtatctgg aagaagatat cagaggattt ctttggttgc aggtctaaca aatggtgagt
    10441 taatcgctcc aatgacttac gaagagacga tgacgagcga cttttttgaa gcttggtttc
    10501 agaagtttct cttaccaaca ttaaccacac catcggttat tagagtaaaa tgaaataaga
    10561 acaggacaaa tcgatcagga cagtaaaatc gaattctaac aatgttttag aagtctatat
    10621 gtactattct agtttcaata taccataact gctcctagtc aacctgcaag tcctgtccca
    10681 gctccaagct cagctccgac tcaagatcct agagtggtag caagcgcttc ggctagctca
    10741 acgagcacac aagctcaaga gcaagttgac aagtctgaac ttcgtgcctt gagtcaagag
    10801 ttagaccaac gcttgaaagc tttggcaaca gtatctgatc caaaaatcga tgcaaccaag
    10861 gctgtcctcc tagatgctca aaaagctccg taagatagtg ccttgacaga gtaaggtctg
    10921 catactgcag tagagtctgt gaaggctgcc ttggactctc tcaaagatgt gaaagcaaat
    10981 gctagcgaca gcaagcctgc acaggacaag aaggatgcaa aacaaggaat ggaagatagt
    11041 aaggattcag ataagatgac tgaaacaaac tcagttccgg caggcgtgat tgtggtcagt
    11101 ctacttgccc tcctaggcgt gattgccttc tggctgattc gccgtaagaa agagtcagaa
    11161 atccagcaat taagcacgga attgatcaag gttctaggac agctagatgc agaaaaagcg
    11221 gataaaaaag tccttgccaa agcccaaaac cttctccaag aaacccttga tttcgtgaaa
    11281 gaagaaaatg gctcagcaga gacagaaact aaactagtag aggagcttaa agcaatcctt
    11341 gacaaactca agtaaaacat gatttatgaa atgtcagacc taaaagacat tttcgttcac
    11401 aaaagcagtg acgagtaatc cttgtcgttg ctttttggct attttggggg ataagatccg
    11461 ccttgactag caaaagaaaa gattggtcca atagtgcccg atacgatttt gcgtagcaga
    11521 aagcttacaa ataaaattga atatagtaaa ataggattag aattattaag aaagttggtt
    11581 ctttattgga aacgatagta tttttaatct ctgtttttct agcaggtgtt ttatcctttt
    11641 tttctccttg tatttttcct cttctgccag tctatgctgg gattttattg gatgatcaag
    11701 aaagtgcaaa aagcttttct ttgtttggga gaaaggttct ctggtcaggc ttgattcgaa
    11761 cactttgctt tatcgctggt atctctctca ttttctttat tctaggcttt ggtgctggtt
    11821 actttggtca tattctctat gcaaattggt ttcgatatgt catgggagct attattatca
    11881 ttttgggtct tcaccagatg gaaatttttc atttgaagaa attagaagtt caaaaaagtt
    11941 ttacctttaa aaaatcagat tctaatcgtt attggtcagc ttttttactt ggtattacct
    12001 ttagctttgg ttggacacct tgtattggtc cagttttaag ttctgtttta gcacttgcgg
    12061 cttctggagg caatggtgct tggcaaggag cgatttatac tctcatttac actctgggca
    12121 tggcccttcc tttcttggtc ttggcactag cttcaggtct agtcatgcca tattttagta
    12181 aaatcaagcg tcatatgatg ctactaaaga aaattggtgg tttcctcatt gttttaatgg
    12241 gaattttgtt actattagga caagtaaatg ttctagctgg aatttttgaa taaaggagga
    12301 caaatgaaaa aagtaatgtt tgctggctta agtctcttgt cattagttgt attgatggcc
    12361 tgtggtcagg aagaaactaa aaagactcaa gcagcacaac agccaaaaca acaaacgact
    12421 gtacaacaaa ttgctgttgg aaaagatgct ccagacttta cattgcaatc catggatggc
    12481 aaagaagtta agttatctga ttttaagggt aaaaaggttt acttgaagtt ttgggcttca
    12541 tggtgtggtc catgcaagaa aagtatgcca gagttgatgg aactagcggc gaaaccagat
    12601 cgtgatttcg aaattcttac tgtcattgca ccaggaattc aaggtgaaaa aactgttgag
    12661 caattcccac aatggttcca ggaacaagga tataaggata tcccagttct ttatgatacc
    12721 aaagcaacca ccttccaagc ttatcaaatt cgaagcattc ctacagaata tttaattgat
    12781 agccaaggaa agattggaaa gattcaattt ggtgctatca gtaatgcgga tgcagaagca
    12841 gcatttaaag aaatgaacta gaatcgataa aatctgatta caagatgtag tcagattttg
    12901 gtaacttcta aaactaactt ccagtttccc acaacttagc ttttagtatg agaaaaacgc
    12961 gtgacatcag tggaaatccg tcttagacta actttcactg gttttctttt tcttgatata
    13021 taagggttca aaagcgaaaa gactcagaaa aagtgcacga caaatagccc taaaacagag
    13081 tcgtcttaga gtaacttcca gttttgggag agagatggaa gttactttga gaagttacgg
    13141 ta
//
LOCUS       DAVLYV010000049        12944 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLYV010000049 DAVLYV010000000
VERSION     DAVLYV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12944
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.049"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(421..2148)
                     /gene="ezrA"
                     /locus_tag="VYO70_001412"
     CDS             complement(421..2148)
                     /gene="ezrA"
                     /locus_tag="VYO70_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1713631.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEEKKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPPQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2230..4176)
                     /gene="gyrB"
                     /locus_tag="VYO70_001413"
     CDS             complement(2230..4176)
                     /gene="gyrB"
                     /locus_tag="VYO70_001413"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1713632.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4191..4754)
                     /locus_tag="VYO70_001414"
     CDS             complement(4191..4754)
                     /locus_tag="VYO70_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1713633.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4839..5393)
                     /locus_tag="VYO70_001415"
     CDS             complement(4839..5393)
                     /locus_tag="VYO70_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1713634.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGNLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5404..6627)
                     /locus_tag="VYO70_001416"
     CDS             complement(5404..6627)
                     /locus_tag="VYO70_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1713635.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFCMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6664..9114)
                     /locus_tag="VYO70_001417"
     CDS             complement(6664..9114)
                     /locus_tag="VYO70_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1713636.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLELRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVFLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9314..10243)
                     /locus_tag="VYO70_001418"
     CDS             complement(9314..10243)
                     /locus_tag="VYO70_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1713637.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKIDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNLIQEGNFSH
                     DQVFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10383..10670
                     /locus_tag="VYO70_001419"
     CDS             10383..10670
                     /locus_tag="VYO70_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1713638.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10704..12038)
                     /gene="ciaH"
                     /locus_tag="VYO70_001420"
     CDS             complement(10704..12038)
                     /gene="ciaH"
                     /locus_tag="VYO70_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1713639.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(12028..12702)
                     /gene="ciaR"
                     /locus_tag="VYO70_001421"
     CDS             complement(12028..12702)
                     /gene="ciaR"
                     /locus_tag="VYO70_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1713640.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
BASE COUNT         3950 a         2758 c         2296 g         3940 t
ORIGIN      
        1 gaggcctatt taccgtggac taaagttgta caagaaaagt gcaaataaga aatctccaga
       61 ttaggaacta tccgtgagtt ctctagtctg gagatttttc aatatacttc gttattggac
      121 ggttaccaag ttactcaaag tacagatttg aagctgcaga taagactgac gaaatcactt
      181 taatactaaa tgaaaatcaa aaagcaaact aagaagctag cctcaggctg tacttgagta
      241 cggtaaggcg acgctgacgt gatttgaaga gattttcgaa gagtataaaa cactgttttg
      301 aggtggtgga taaatcggct gggtaaaaac tcaatctcag gagaaaatga agtaaatctc
      361 cccacaacta tagaaaaaga tttgattctg ctcctcacac aataaaatct ttttctttta
      421 ttaaaaacga atcgtttcac gtgttttctc atatgaggta acaaagcgat tagttacacc
      481 aggctctgcc acttccaatg cttgagaaat cttgtcaaat gaagcgtgat aatcaaattc
      541 tttttcaaaa atatctaaag cttcgttaaa tgcttcttga atgcgttcat caaatgagcg
      601 atagcggtta gaatattgca agagttgctc tgtcaaagtt gcatattgta caatattata
      661 agtttccgtt tctaaagctt ccatatcatt cgttgcaatt tcaagaactc gggtaacaga
      721 ttcaatgtta atcatttttt gttctaactc aaccattaaa tcctcggtat tattgcttgc
      781 cgtaaagaat aacttcaaga aagtttgtgg aatacctggc agattgcgtt tttccatgta
      841 tcgcttgata gtatggagac gattgacata aacattggcc ttttgacgtg cattaatatc
      901 atctttctca atttgtgtca ggcgctcact aactgaaatt tgctcatctt caatatcttt
      961 tagttgagtt tgtaaatcct caagattttc ttcaagaact gaataagctt ggggtggttc
     1021 ttcttgattt gaagttacct caacaatagc tgcctcaaaa ctctctaatt ctgtctgaat
     1081 acgacgaaca tggcttgcag ctgtctcagg aagtaaatag gtcttgttca aacgtgcaat
     1141 atcttctccc aataaagtat tattctcttt catatgttga agataagttg gaagagttgc
     1201 aagtagattt tccactactt tctgagcagc aatttctcga gtaaaaatat catacaaggc
     1261 attgatttcc tcttgtgcct gtccattctc atattcggca ttatccaatt ccaactgacg
     1321 aatattctct tggtttttct tgaatgcttc ataaagcaag tggaaacgcg cttcaatatc
     1381 cgtttcaaca aaatgataat tagcatcaat tagtttacga taaccggctt ccaaatcctg
     1441 taattgatct ggcaattctg tagaaagcgt cgtaaccaag gctggaacac gatccacaat
     1501 atgacttaag gccaaaatgt gattttctgt attatccaaa atcactgcgg cttccacagg
     1561 gtcacccgat gaattcaagg ttacaaattg tgaaaattca gattggatat tttctaattg
     1621 tttttcaatt tcatccaagg cttgaccata ctgttctgaa ttttcagcaa ctctatgctg
     1681 aagttcctca aataaatcca aagcatgaag aacacgacca ctatttttag attcttgctt
     1741 ctctaagtct gccaaagcat tgcgaattgc cgcaatatct tcttcaatca aagtaatttg
     1801 actctcaatt tggtcaattt gatgactggc cttgagaaaa cgaaatgaat ggttataacc
     1861 ttctgcttca aagagattat tttcaatatc ggcaaaagag ttgagagata aatcgaccca
     1921 tttttgattc cattcacgga aagccacttg actttgtcca atcaagtgca tattttttac
     1981 agcttctact tcatcattta ctggaagatt gtatagttct tctttttttt cttctagcgc
     2041 ctctaatctc ccctcgtttc gcttacgtag aaagattgcc actacataag ccagaactaa
     2101 aatgactgca attgcaacca ttaaataaat tagttgtcca ttagacatat caaactcctt
     2161 ttttacttga aacaatcgta acgattatat catatttttt agaccaaggg aactacttct
     2221 ccctattttt tagacatcaa gtgtactata gacagcattt tcttcgataa actcacgacg
     2281 aggctctact cgatccccca tcaacatatc aaagatttta tctgcttctg cagcatcatc
     2341 tacagaaact ctagccatca agcgatgttc gggatccatg gttgtttccc acagctgatg
     2401 atcgtccatt tcacctagcc ccttataacg ctgaatagtc ggtttggtac gaccttcact
     2461 ataacgggct aaagcttctt ggagtttgat ttcttgatct gcacccggct ggatatattc
     2521 tttaatctcg cttccaacct tgacaccata gattggtggt tgggcaatat aaacataacc
     2581 agcttctagg attggtttca tataacgata aatcaaggtt aaaagaaggg tacgaatatg
     2641 ggctccatcg acatcggcat cggtcatcaa aacgagtttt tggtaacggg ctttcgaaac
     2701 atcaaattct gcgccaaatc ctgttcccat ggctgtgaaa agactacgaa tttcttcgtt
     2761 agctagaatc ttatccatac ttgctttttc aacgttcaaa atcttaccgc gaattggaag
     2821 gatagcctga aactcacggt tacgaccaga tttggctgat ccaccagctg agtctccttc
     2881 gacgatgaag agttctgttt cagcagggtt attagaagaa cagtctgcta gtttccctgg
     2941 aaggttggaa atttccaaac cagatttttt acgtgtgact tcacgcgcac gcttggcagc
     3001 cacacgagcc ttggcagcta aaatcccttt ttccacgata cgtttggcaa tctgtggatt
     3061 ttccatgagg aaatcggaga aggcttcact gaagaggcga ttggtaatct tgaccacttc
     3121 gctatttccc aatttggtct tggtttgtcc ttcaaactgt ggatttgggt gtttaactga
     3181 gataactgca gttaatcctt cgcgaacatc ttcccctgtt aaattatctt cattgtcttt
     3241 cagtaactta ttcttacgag cataatcatt gataacacga gtcagtgccg tacggaaacc
     3301 ttgttcatgc gttccacctt catgggtatg aatattattg gcgaaactca tgacattttc
     3361 atggtaacct gttgtgtact gcatggctac ctcaactgta atatcatcca tctcaccgtc
     3421 tgtatagatt ggtgtatcaa agattacatc cttgttctcg ttgatatatt caacgtaact
     3481 agcaatccca ccttcataat gataatgctt ggtttgttcc aaaccttggc gcttatcagt
     3541 gatagaaatt tgaagaccgc gatttagaaa ggccaactct tgaatccgtt tatttaattt
     3601 atcaaaatca aaggttgttg tttcagtaaa gatttttggg tccggtatga agtgaaccgt
     3661 tgttccagtt ctatctgtat ccccaaccac ctcaaggtct gcaacaacat gaccacgacg
     3721 gtattcttgg taatgaatct taccgttttt atggacatga acgtctaatt gagtggaaag
     3781 ggcattaact actgacgacc ccaccccgtg aagaccacct gaaaccttgt atccaccacc
     3841 gccgaacttt cctccagcat gaaggactgt aaagacggtc tcaacagcag gacggcctgt
     3901 tttttcctga atatcgactg ggataccacg cccatcatcc acaacagtaa tcgaatcatc
     3961 tggctcaata aaaacttgaa tatggctggc aaatcctgcc aaggcctcgt caattgagtt
     4021 atcaacaatt tcccagacta gatggtgaag accttctttt gaggttgatc caatgtacat
     4081 ccctggacgc atacgaacag cctctaaacc ctctaaaact tgaatttgac tggcatcata
     4141 atcctgtgcc tgcagatttt tgatttcttc tgtcatctta ttcctttttc ttatatgtct
     4201 aatacttgtc ttaaattcac gatactggta aacaagtggg tatcaagtcc tgcagcttga
     4261 cctgcttcga tatcaatcgg ccgatcacca atgacaagac cagagctaat ctgatacttt
     4321 tctcttaaat aaagcatgga ttcgggattt ggctttctct taaagcctga gctagaagtc
     4381 accacttctg taaaataagc tgctatagag gttttttcta aaatttccaa aacctgatca
     4441 tttcgatgag agaccaaaaa atgacggcca ccttgattta aaatgtcttc caataggtca
     4501 gaaactcctt caaataaaat cgggtgttca agctctctgg cttcattttc cttgtacttt
     4561 tctaaaaaat tctctaaatt gggagcgaat gtctcaatcg caaaaggagt agaaaccttt
     4621 aaagcttgat agacactgtc atggtcttgt gtgataccat acagtgccaa tgtttcaaca
     4681 aatgcagctg ttgaagtttc ataattatcc agtaaagttc cacctaaatc ccagatgtaa
     4741 tcgtgatatt tcataccctt cattatagca tttttttatg aaaaaagcga tgatttccct
     4801 gagttttcca cataaaaaac gagagaataa ctcccgtttt actgattttt accaaagaca
     4861 tctcgataga gcattcctgt tcgtaaactc tctgcgtccc ctcctagttg ctccaccaac
     4921 tcgtaggcaa aggcaagggc tgttgaagga ccccgactgg ttgtcaactg accatctacc
     4981 actactgttt ccttgacgta gtgaccatca aggatttgct cttgaacgcc gtcataacaa
     5041 gtgtatcgct tatttttcaa tatctctgct tgattgaggg caattggtgc cgcacaaatg
     5101 gctgctagtt tcttcccttc ttgctcgaag ctttgcaatt cttgaatcaa ggtctgatta
     5161 tcacgtaaat gtgcagaacc aggcatacct ccaggaagaa caatcatatc atagtctgat
     5221 aaatttccat caaagacatg atctgctctt acttggattg catgcgaacc cgttacttgc
     5281 tcttcaaaac caaccatatc acatgtgata ttggctcgac gcaagacatc tacaactgtc
     5341 aaggcttcaa tttcttcaaa gccctgagct aacataactg ctacttttac catgattttc
     5401 tccttattta atttgtttta atacaacctt tttccgtttg aatgggactt ttccgctctt
     5461 ttcttcagct agattagtct ggtaactcat cgataaaagc aaaccaacac caatcagatt
     5521 actgataata gctgatcccc cttgcgaaat gaaaggcaag ggaatccccg tcaaaggaag
     5581 tagtccagtc acagcaccga tattctcaaa gatgtggaag agcaacatca taatcaaacc
     5641 tgtggaaata taagtgtaga actggttatt tgatttaaga gtaatcttca acatacggta
     5701 aatcaacatg agatagaggg caataaccag gacagagcca ataaagccaa aatcttctgc
     5761 aataaccgta aaaatcatat ctgactctcg aactgggata agcagattcg aagcattaaa
     5821 tccctgacca aataagccac cactcccaat ggcaatctgc ccttgagcct gctggtaagt
     5881 cgttgtttgg gcaaactcaa agggattgag ccaagccaaa atccgattaa tttggtaggt
     5941 cggcattcca atctggtgaa gaaaagctcg tccgtccttg ctaataaaga tagctaagaa
     6001 accagcaact cctgttacag cagtcacaaa tactgggata ataattttcc aagaaacccc
     6061 tgataataaa acgattcctg agaaaatggc tacaaaaacc aaagccgtcc ccaagtcact
     6121 ttgaagtgct aaaagaacta ggactggaat ggtaaagaga atcatacaga aaattaacaa
     6181 aaagtccagc ggaaccgtgc gtctccattc cttatgtttc tttgtaaatt ggacaatgac
     6241 acgagccaac atgaggatat aggatatctt cataaattct gacggttgga atagggtaat
     6301 tccatttatt gatacccagt ttttggcacc cgttgatgca actaagcttg gattataaaa
     6361 tacaatcggc aagatcataa gtcccaagcc taaaatatat agaaaggggg tcaccttcca
     6421 aagaaattct gtattaaaga gcatgaccac aaaaccaatc acaagcccca aggcaatcca
     6481 ggcgacctgc tgccctaaaa tgggcagaat attattgggg taatcatgac taacggctat
     6541 atagatagcc accacaccga tgaccagtag aaaaaatact ggcaagagca aactgtaatc
     6601 gactcttgag tcgagagaac gtttcatata tactaacctt atactttcat acaatactat
     6661 ttatcaaagt tcattaaaaa atctatcaat agcctcgtca acttcggatc gagagatggt
     6721 tttaacagtc gcttcttctg ctagagatgc tactatttgt ttgccgtatc gttttccgac
     6781 gattctccta tccaaaataa gagttaagga acgttggtat tcacgtctca tacttcttcc
     6841 caaagcctgt tttaaacgaa taatggccat tggcaattga taatcataaa aggcattttt
     6901 cccttcttga ttcagttctt gattaatctt tttcgtcaag ggttcttgag gattttggaa
     6961 aggaagcctc ggtacaactt gaatcacaaa aggatggctt gaaaaatcaa ctccctccca
     7021 gaaacttgct gcaccaagca agatttgttg ttcacctttt tcaaagcgtt tctttagctg
     7081 atgaacatcc ccatttttat actgggccaa gtggctaact gtaagtaaat ccgatactgc
     7141 tagaagcatg tctttagcgg taaagagaac caaaatcggt tgctggaaag cttgaatttc
     7201 cactagtaaa gcagctacct ctctggcata gacttctaag gaggtttctg ttaccagagg
     7261 gaaatctttg accatgacca cttcttgttc ctgttttccc cgagattcaa tcttgacaaa
     7321 tttagcttca ggatagccta aaaggtctgc caaagaaacc ctctgactaa tctcaagagt
     7381 agccgatact cccaggactt ggcaactctc aggaagtaaa gaggacagaa gagtacggcc
     7441 tgattttgta gaagaaatct gaattttctt ttgactcgtt tcagttactt caagccagta
     7501 atcaccttca gctgtaaaat agcgaaccag ctcatcaaag ccttctactt ctaattctga
     7561 aaaatactga tggagattgt ccagagaatc taagatattt ttcctagatt taccagactg
     7621 aaattgttct atcaagtaga gacactcaaa gcgaatactt tctagtatcc gttgttgaac
     7681 cctgttttct tctcctacta aagccttatc aattaaatcg ataatagatt gtatatcgta
     7741 ggtctcttga agcagatttt ctagagctaa caaaatcttt tggacttcat caataatcag
     7801 taaacggtca ctgacaaatt caggattatc ttcaagtctg gtcacgagat aggcatgatt
     7861 agtcactaaa agcttgcaag tctctgccct ttcttgacta cgtttccaaa aatcttccgt
     7921 cacaaataag ctctgggatg ataaattccc atcatgacga aggtctgcta gaaaatgttg
     7981 gtaacggtag agttgcccga tttcatccaa atctcctgtc tctgtctcag taagccagac
     8041 caagacttgc attttaaagc gtctaaataa gcgattttca tcattttcct gcaaggaatg
     8101 ataaaaggca tccaacttca gataattttg tggtcccttt aagctatgaa tatctgtatg
     8161 gaacacttcc ttgagacgtt taccttcttc ttccatgatt tgattttgaa gaatctttgt
     8221 cggaacacta agaacaattt gtcgctcttt ggattgagat aaagcgggta agagatagcc
     8281 ataggttttc ccaatccctg tcggcgcttg aatcagaaag acaggttcat ctttcaatag
     8341 caagccaacc tctttagcaa aactttcttg ttcctccctc acttcaaggt tcaacagaga
     8401 aatattttta gaaaagtctt gagatagttt tcgtagctcc agagaagctg ccgttttctt
     8461 aaaatacaga ccttgaactt ggaccaagtc tggagaactc aggatagatt ggttgcgata
     8521 agtttcctca ataaccaggt aggactcata taggagagcg tcagccattt ccagcaagcg
     8581 ttccaagaga cctttaggaa gctgggtcat cttttcccgt aaaaaaagaa gtaattctgc
     8641 tgtagcttgg gcatctgaaa gggctgtgtg tgcgtgttta agaggaattc ctaattctcg
     8701 acacaaaatc ggcaagctat atttttccag ttcagggaaa aagacctggg ccaattcgac
     8761 cgtatcaaca cgagggtttc ttagctcata gccttcaaaa aataaatttt ccgccaagag
     8821 attagcatca aactgaacat tatgggctac aaaaatccca tcctccacca agtcaaatat
     8881 ttttctggca acttgcgaaa aatcaggtgc ttgcgccaga cgttggtctg tcaatcctgt
     8941 cagttctttg atatgagcat ccaagggttc atgtggattg acatccgtcg tatagtgatc
     9001 gacgatttct ccgtcctcaa tcacgacaat tcccacttgg ataattttag ccttactacc
     9061 tgtgctagtt gcctctaaat ccaccacaac atagcgatta ccaatcgttt tcattataaa
     9121 aaattatacc aaaaaaaggg ccatttggca cctgcaaaca taggataatt ttcaaactca
     9181 agaacactac ttttcttaaa aatcccatcg aatagagcat tctagcctgc tgatttttgt
     9241 aaaatagtga agaaatacga aaaattgaga gaagaaatgt ttgcatcttc cttctctcaa
     9301 cgaatcatgt attttatcta accatgtcag gatcgtagtc ataaaatcct gtatcaagga
     9361 aacggttttt agggtgaagt ttacgaactt cttcatccag caagaagact tgatcatggc
     9421 taaagttgcc ctcttgaatc aagttacgcg cttggataaa gagttttgca atctcaacaa
     9481 agttctgacc aggagtgtag agcccttcta gtttccagcg agtaaagcca tgctccacca
     9541 attctgttaa tttgatcatc aaatcaaggt cattgttggc aaagatatgg gtcccatgat
     9601 tatcttcaaa aatggaatag tggctctctg gatcacttgg ctcagccaag aagaggtcac
     9661 gtttatgcgt cttttcatca tcgatatgtg taaagttata gtagttttgc aagagtggac
     9721 gtttagaatg atggatgacg ctagcaccgt aaaccaaaac ttcagcagga atttccaaaa
     9781 tctctggcat tttgaaaagt tcagctgatg gaatttcacg cgccaaaaca gcctcagatg
     9841 cgccagcctt ttgtccccag aagttaatct gacgactgct agttaccatg gttgaagcat
     9901 cgtagatggt cttaaatgaa taaccatcgc ggttaactac gtaaaagacg cctgcatccc
     9961 caatcgtaat atagtctatc ttgatttctt ccaagaagtc taagaaaggc ttgatacggt
    10021 ccatcatatc ttggtgcatg agagcattga ccgcaacgag caattcctta ccagcatcat
    10081 gaaccaactt agcgatttca cgtaattggt cataactaaa ggtcgttggc agacgaaggc
    10141 caaaatcttt ctcaccgaca tagatacggt ctacgccagc ttcgagtagt tgttcaactt
    10201 gttcaatact ttcagcagtt gctgtaatga caatcttttc cataagaaaa attataccac
    10261 atttctccaa aatcatccat gctttgaaaa tgaaaaggga ctttattctt tttgtaacct
    10321 ttctgtaata attctctgcc gaaaaaatga taaaataggt atgtactgtt aaggagagaa
    10381 ttatgcccgt aagaaaatta caatcctatg aggtagacta tcaagaagaa ttaaaccagc
    10441 agcttcctca ttaccaagct tatacacctg aagcgcaatc tgatgccaat ctcaaggaaa
    10501 tcctattttt tattaatatc gctgtttttt gtatctgtat tgctatcttt agttttatct
    10561 ttttagcatt aaaattatca actgctcttg cctttgccgc agcaatcgga ttcagcttac
    10621 ttgttttaaa agttcaacgg tctattatca aacgaaaacg tagaagataa atccaaaata
    10681 tcgccccaat tggagcgata tttttatttt ttctttttag atggtgtctg aatggcaatc
    10741 ttcacttcaa agattgttcc cttgggttta ttatctttga cagtaacagt tccttttaga
    10801 gcatctacaa tttgcttggc tagggataat cctaaaccaa aaccaccttt ttgccgggtt
    10861 ctagccttgt ctactcgata aaaacggtca aaaattttct ttttatcttc tgtcgaaata
    10921 ccgattccat tatcagaaac aagtaaataa agattgcgat cggtcgccga gataagaaaa
    10981 tcaatttcac catcctcctc agtatacttg acggcattat cgaaaagaat ggtcatcagt
    11041 tgtttcagaa gaagctgatc tgtgacaatt gttcgatgga tacgattttc aaaacggaag
    11101 acacgattat tttccgaagc aatcatctca tagtttgtga aagttgtatt aaaaaagcta
    11161 gttggaactt ctgcaagctc cggcttaatc ccatcatctc tccgagctaa gttcagcaag
    11221 cttgtcgtta aaaaacgcat atttcggact tcttccaaac tcgatgcaat gctttcgctc
    11281 acatccataa tggtagcttc tggcttacga aaaagggtct ctaagcgatt ttgcaaaact
    11341 gcgagtggag ttcgtaactc atgactggca ttttccacaa aagactgttg cttctgcata
    11401 ctctcaagca ggggcctaac actgacccta gctagataga gactggcaag taaagacaaa
    11461 atccagaaac tagccatcac gaccacaatc aattgctcat gcttttgact agcctgctcc
    11521 aactgactgg tattaatcaa gacagcagca tacttgatat tggttgaaac cgaactaata
    11581 ttggtctcca tcaaaatcac acgatagatt tcttcctgcc catagctatt aaaaacctga
    11641 atctggtaga tatgtcctag ttctttcttt tctaacttaa tcttatccaa gcccaaaaat
    11701 cgatttccag aaagaagttg agtaaagtct ttatcaaaga gaatgacttc cgtattggaa
    11761 ctgacattag gttttatttc tactttacta gcgtccgccc tagcattttc taaatcttta
    11821 atctcttctg ttgccctatt tatagccagc tgaataactg cttgaggatt ttcactcagt
    11881 ccatgaagct tatcgtccac cgaagtataa agactcgaat gcatgacttg taaaataatc
    11941 agagtcattg tagaaaaaat cagggtgaag acaccgaagt tgcggataaa ataactaaag
    12001 tcatccgcat accatgtttt tttaagttta ctgaacatct tttaaaagat acccaacact
    12061 acgcaaagtt tgcaaattct ctgcaaaagt ggttcccttt aatttcttac ggacttttga
    12121 aacatagact tcgacaaccg aaatcgttgt atcactatca aatccccata gacggtcaaa
    12181 aatctgcgtc ttaggcaaaa tcacattttg attttgaagg aaataaacta gtaaatcgaa
    12241 ctctttcccc agcaattcga caggagtatc ttcaacttta acggtattgg ttgataaatt
    12301 aaccacgata ttcccataag tcaaggtgtt ttcattaaac ttccctgaac gtttgagaag
    12361 ggcctgaatc cgcattttaa gttcttctag gtagaaaggt ttggtcagat aatcatccgc
    12421 tcccagttca aatccatgtc ccttgtcatc caaactttcc ttggcagtca taatcagaac
    12481 tggtgtcgta attccctttt cacgcaattc ttttaagact tggaaaccat ttttttctgg
    12541 caacatcaaa tccagcaaaa tcaagtcata gacaccactc tcagcttcgt agagaccttc
    12601 ttctccatca aatacctgca taacatccgc aaaatcgtct aaaaagtcaa atactgaatt
    12661 tgacagacct aggtcatcct caaccaataa gatttttatc atgagaaact cctccttatt
    12721 aaaactatta taccaaattt gccttaaaaa aaactcaact ctctgcattt tacggctcta
    12781 taatatttgt agtgggtaaa tcccctatag atattatgga gcttattttt gtgtagaaaa
    12841 aaagtcccat atgacctata atgaaaagcg atcaaacaac tcattagaaa gattcatatg
    12901 ggacaattac attttatcac aaaactactc gatatcaaag acac
//
LOCUS       DAVLYV010000050        12603 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLYV010000050 DAVLYV010000000
VERSION     DAVLYV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12603
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.050"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..1109)
                     /locus_tag="VYO70_001422"
     CDS             complement(<1..1109)
                     /locus_tag="VYO70_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1713641.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIIEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKRT"
     gene            1167..2333
                     /locus_tag="VYO70_001423"
     CDS             1167..2333
                     /locus_tag="VYO70_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1713642.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            2472..2798
                     /locus_tag="VYO70_001424"
     CDS             2472..2798
                     /locus_tag="VYO70_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1713643.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            2785..3378
                     /locus_tag="VYO70_001425"
     CDS             2785..3378
                     /locus_tag="VYO70_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1713644.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            3371..4282
                     /locus_tag="VYO70_001426"
     CDS             3371..4282
                     /locus_tag="VYO70_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1713645.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDFITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTDNHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            4344..5408
                     /locus_tag="VYO70_001427"
     CDS             4344..5408
                     /locus_tag="VYO70_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713646.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADFDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEVGEARTEIEEKKASNSTEEESDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(5586..6446)
                     /locus_tag="VYO70_001428"
     CDS             complement(5586..6446)
                     /locus_tag="VYO70_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1713647.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPLSFYHQLDANQIEVQPILD
                     KDFKALAQEE"
     gene            complement(6568..7554)
                     /locus_tag="VYO70_001429"
     CDS             complement(6568..7554)
                     /locus_tag="VYO70_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1713648.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(7826..9301)
                     /locus_tag="VYO70_001430"
     CDS             complement(7826..9301)
                     /locus_tag="VYO70_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1713649.1"
                     /translation="MKNWKKYAFTSASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(9559..10482)
                     /locus_tag="VYO70_001431"
     CDS             complement(9559..10482)
                     /locus_tag="VYO70_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1713650.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(10496..11425)
                     /locus_tag="VYO70_001432"
     CDS             complement(10496..11425)
                     /locus_tag="VYO70_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1713651.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(11744..11965)
                     /locus_tag="VYO70_001433"
     CDS             complement(11744..11965)
                     /locus_tag="VYO70_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713652.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            complement(11919..12179)
                     /locus_tag="VYO70_001434"
     CDS             complement(11919..12179)
                     /locus_tag="VYO70_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713653.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         3762 a         2679 c         2310 g         3852 t
ORIGIN      
        1 gttctcttgt actccttctt ccatttaaat cctctaatta aacctacaat cgtcccaaag
       61 ccataagcaa agtgaatgga aaataaaaga aagggcatca caattagaaa tccattttta
      121 tgttttaata aagtcagcaa agtgagtagt gacaaaagta gaaaataggc acctaatagt
      181 aaagttatga atacgaatgt gatcggtaac aatgctagac taaacacaag actcaaaaca
      241 aataaacaag gaacatagtg aaataatgat aaacacttaa actgaacatg acttgtcaag
      301 ccaatccaca aaccatttaa atacttttga tgcagcattt tcttgaatgt tggtcgaata
      361 tactgataag atagaatact tgggctatag cggattttat aaccatattc tcgaattcta
      421 taatgaatat cattatcttc agttcggcca agttgctcat ttactaaacc aaccttctgg
      481 aaaacctctc gtttatacat tccatgaaaa atagaagaaa catatctatc ctcagaacta
      541 tttcgataat tggcaatgct actgccaaac atattttcct caacaagatg caaggtctct
      601 gcccattttc cttttccttc gataatcgtc ggtctaggcc ccccacagac aaattcacct
      661 tgttgaataa tagccacatt gttcattaca aaactctcag taacttttga atgagcatca
      721 atttttaaaa taaggtcccc tacagaatgt ttaactccca ggttaaaacc actagcttga
      781 tttttcttag gattgttata caatctaatt gagttaaact ctgtatcttc ctttataaat
      841 tgctgaatga tagctgtggt cccatctgtg gacatagcat ttataaatag aatttcaata
      901 ttctctttag gataagtttg attttttaag tcttcaatta gaccaggcag atatttttct
      961 tcattgtatg cactgattac aatggatacg agaagacttt caacattttg agtcactact
     1021 actctcctga atttttcctt ccaccattct atcataattt tcaagacttt cccattttta
     1081 tcttgaaagc cagaaacctt acttgacatt atagtgaaaa agccttaaaa taagctgtta
     1141 ttaaaatcag ctagaagagg tattatatga aaaagcaatc actctttttt gttccaggta
     1201 ttatcctgat tggtgtttcc ttgcgaactc cttttactgt tttacccatt attttgggaa
     1261 atatttcgca aggtctggag gtagaagtta gttcgcttgg tgtcttgacc agcctgcctc
     1321 tccttatgtt taccctcttt tcaccatttt ctacccgact ggctcagaaa atcggcttgg
     1381 agcatctctt cacctacagc ctcttcttct tgaccatcgg ctcactcatt cgactaatca
     1441 atctgcccct gctctatcta ggaaccttga tggttggggc aagtgtcgca gtcatcaatg
     1501 tcctgcttcc tagtcttatc caagccaatc aaccaaagaa aattggtttt ctgaccacct
     1561 tatatgtaac gtctatgggg attgcaacgg ctctggcttc ctatctagct gtgcccatta
     1621 cacaagccag ttcttggaaa ggacttatcc tcctcctcac gttactctgt ctagcaactt
     1681 ttttggtctg gctcccaaat caccgctata atcatagact agctccacaa accaaacaaa
     1741 aaagtcaaat aaaggtcatg cgtaataaac aggtttgggc aattattatc ttttcaggtt
     1801 ttcaatcctt gatcttttac accgtcatga cctggttacc taccatgtct atccatgcag
     1861 gtctatccag tcacgaagct ggcttgctga cttctatctt atctctgatt agcattcctt
     1921 tttcaatgac catcccaagc ctgacaacca gtttatctac tcgcaaccgt cagctcatgc
     1981 tcactctggt ttcactagct ggtgtggtcg gcatttccat gctctttttc ccaatcaata
     2041 atttcattta ctggcttacc atccatctcc tcatcggaac cgcaaccagc gccctcttcc
     2101 cttatctcat ggtcaacttt tcactcaaga caagcgcccc tgaaaagaca gcccaattgt
     2161 ccggcctatc tcaaacagga ggctatatcc tagcagcctt tgggccaacc ctctttggtt
     2221 acagttttga cctgttccac tcttgggtac cagctgtagc tgccctcttg ctcatctata
     2281 tcctgatgac tgtggccctc tttacagtgg acagagcgga taagatcctt taaacttcta
     2341 gttttagcta gaagtttttt atatataaaa attttacaca tttctcttga cagggctttc
     2401 ttttagatgt acaatgtatg tgtagaaaaa ttatatataa aaatcttaca cattagaaaa
     2461 ggaggtttcc catgtacttt ccaacatcct ctgccttgat tgaatttctc atcttggctg
     2521 tactggagca gggtgattct tatggttatg agattagcca aaccattaag ctgatcgcta
     2581 atatcaaaga atccacactc tatcccattc tcaaaaaatt ggaaggcaat agctttctga
     2641 caacctattc tagagagttc caaggtcgca tgcgcaaata ctactccttg acaaacggtg
     2701 gtatagagca gctcttgacc ctaaaagatg aatggacact ctatacagac accatcaatg
     2761 gcatcataga agggagtatc cgccatgaca agaactgaat acctgactca gctagaactc
     2821 tatctcaaga aactacctga agctgaccgt atcgaagcca tggactattt cagagagctc
     2881 tttgacgatg ctggagtcga aggagaagaa gaactcatcg ctagtttggg aactcccaaa
     2941 gaagcggccc acgaagttct atccaatctt ctcgataaaa aaatcaatga agcacccgct
     3001 caaaaaaata accgacaaat tttacatatc gccttgttag ccctccttgc agcacctatc
     3061 ggcattcctc tgggaatcgc catcctcgtg accctgttcg caatccttgt agccgctttg
     3121 actgtcattc tggctttctt tgcagtttcc atactgggta tcatcggcgg attcctattt
     3181 ttagttgaaa gtttcactat cctcgcccaa gccaaatcag cctttatctt gatttttggt
     3241 tctggtttac tggctatcgg tgcttcttcg ctagttttac ttggcatttc ctatgtagct
     3301 cgcttcttcg gtctactcat tgttcgtctg gtacaatttg ttcttaaaaa aggaaagaga
     3361 ggtaatcagc atgcgtaaat ggacaaaagg atttctcatc tttggtgtgg tgactaccgt
     3421 tatcggcttt atcctgcttt ttgtaggtat ccaatctgac gggattaaga gcctactttc
     3481 catgtccaaa gaacctgtct atgatagccg tacggaaaag ctaacctttg gcaaggaagt
     3541 cgaaaaccta gaaattactc tccaccaaca cacgctcacc atcacagact ctttcgatga
     3601 tcaaatccac atttcttacc atccatctct ttctgctcac catgatttta tcaccaatca
     3661 gaacgataga actctgagtc tcactgataa gaaactgtct gaaactccgt ttctctcttc
     3721 tggaattggt gggattcttc atatcgcaag tagctactct agtcgttttg aagaagttat
     3781 tctccgacta ccaaaaggga gaactctaaa agggatcaac atctcagcca atcgcggaca
     3841 aaccaccatc ataaatgcta gccttgaaaa tgcgaccctc aatacaaaca gctatatcct
     3901 ccgaattgaa ggaagtcgta tcaaaaacag taaactcaca acgcccaata tcgttaatat
     3961 ctttgataca gttcttacag atagtcagct agagtcaaca gataatcact tccacgctga
     4021 aaatatccaa gtccatggta aggttgaact gactgccaaa gattatctca gaatcatcct
     4081 agaccagaaa gaaagccaac gaattaactg ggacatctca agtaactacg gttctatctt
     4141 ccaattcaca agagaaaagc ctgaatcaag aggtacggaa ttaagcaacc cttacaaaac
     4201 tgaaaaaacc gatgtcaagg atcaactcat tgcgagatct gatgataata ttgatctaat
     4261 atccacacca agcagacgtt gacaaaaacg cttacatctg ctactatgga agcatattta
     4321 tctaaccaaa aggaggttct accatgaaac aagaatggtt tgaaagtaat gattttgtaa
     4381 aaacaacaag caagaacaag cctgaagagc aagctcaaga ggttgcagac aaggctgaag
     4441 aaacgatagc cgatttcgat acaccaattg gaaaaaatac tcaagtagag gaggaagtct
     4501 ctcaagctga agtcgaactg gaaagccagc aagaagagaa aattgaaact cctgaagtcg
     4561 gtgaagcgag aacagaaata gaagaaaaga aggcatctaa ttctactgaa gaagagtcag
     4621 acctttctaa agaaacagaa aaagtcacta tagctgaaga gagccaagaa gctcttcctc
     4681 agcaaaaagc aaccacgaaa gagccacttc ttatcagtaa atctttagaa agtccttata
     4741 tccccgacca agctccaaaa tctagggata aatggaaaga gcaagtgctt gatttttggt
     4801 cttggctagt ggaagcgatc aaatctccta caagtaagtt ggaaacaagt atcacacaca
     4861 gttacacagc ctttctcttg ctcattctgt tttctgcatc ttcctttttc tttagtatct
     4921 atcacatcaa acatgcttac tatggacata tagcaagcat taacagtcgc ttccctgagc
     4981 agctagctcc tttaactctt ttttctatcg tctctatcct agtagcgaca acactcttct
     5041 tcttttcatt cctcttgggt agtttcgttg tgagacgatt tatccaccag gaaaaggact
     5101 ggacgctaga caaggttctc caacaatata gtcaactctt ggcaattcca atcttcctca
     5161 ctgctattgc tagtttcttt gctttctttg atagcctacg atttacagct ctcttgtgtg
     5221 tgattagcat tggaatcatt ctgcttgcca gtctccatat cattacaaga ccaagtcaag
     5281 caagtgaaac cgactccttc tatcaattat tcttgtctgt ccttgtgaac ggagttatta
     5341 tcctcctctt ctttgtagct gaagtggcac tgattggaga ttatcttcgt atcttggcct
     5401 ttctttaaac ttcatactct tcgaaaatca aattcaaacc acgtcagctt cgccttaccg
     5461 tactcaagta cagcctgcgg ctagcttcct agtttgctct ttgattttca ttgagtagca
     5521 atcctgtcat ccttggcagg attttttcta tgtcaaaaaa gcaagtcgat tgacctgctt
     5581 ctgctttact cttcttgtgc taatgcttta aaatctttgt ctaatatggg ttgcacttct
     5641 atttgattgg cgtctagttg gtggtaaaaa gataggggta atacctctaa gaattttgca
     5701 tagtccaagc gggcaatggc ttcatagtct tctggtttag agccatctcc tgtgatttga
     5761 accaagtcaa tctcccactg cacttcctta tccaccaatt gctcaatata ggctgcagga
     5821 acaaacggtt ctctcggtaa aactgtcttg actccttgag ctagatggta aacgccatgc
     5881 acttttcctt ctccatcctg atagaaggaa actctcgcaa agtaggcatc tgtctcttga
     5941 accgcacgca cacgcgcttc aaactgagcc aagccatctg cctctaaaaa gcttttcaag
     6001 ttctcacgac taaagaaaat aggattaaaa attccttggc aaatcgtaaa acgagctaca
     6061 gggtctgcta gataggcttg aatacttgct tggaaataag cttcaaaatc aaaaccatct
     6121 agcccttcaa tatcagtccc tgtataagca agcaaagcat ctaaaaatga cttgataatc
     6181 tgccagtctg tctttgttag taggtcagga agatcgacac ggtaagcctt ttgaccatca
     6241 gcataactga ccttaaagag aaattgtgat tgccccacta tcgcacactc gatatattcg
     6301 agacgattaa gaggctgacg gagatagact acatcatagc tatgtgactc caaaccgtct
     6361 agcaaggcca agatcgactt ggcattcaaa atctcttgtt ctcctaaaat gctctgttta
     6421 tttggaataa aaaatgtttt tgccataact ggactccttt gaaatcgttt acatacagta
     6481 taccttattt ttctgaaaga tacagaaaca aactttagat ttttgagtac aaagcataga
     6541 aaaacagccc ctgaggactg tttgatctta tacagtagct gcttgatcca agctttcacc
     6601 gatagcggct aggcgctcaa tcacttcagc ttgtgtcaat tcattttttg aaacatagcg
     6661 gttacgtggg tgaacacggc actcgtgtga gcatccacga aggtacttgt cttcattttc
     6721 ttctgatgtc aagatacgac ggttacagaa gggatttcca cagttgacat aacgttcaca
     6781 tggtgttcca tcaaaccagt ctttccctac gatggttggg ttgacatggt tgacatcaac
     6841 ggcgatacgc tcgtcaaata cgtacatttt tccatcccaa agctcacctt gaacttctgg
     6901 gtctttaccg taagttgcga ttcctccgtg caattggccg acatctttgt agccttcacg
     6961 gaccatccag cctgagaatt tctcacagcg aacgccacct gtacagtaaa ccacgacacg
     7021 cttgtccatg aatttttcct tgttatcacg gacccattgt ggtaactcac ggaagttgcg
     7081 aatatctggg cgaatagccc cacggaaatg tcctaggtca tactcataat cattacgtgt
     7141 gtcaaggaca acggtatctt tatcaagaag cgcttctttg aactcttttg gagacaagta
     7201 agcacctgtt gtttcaagtg ggttgatgtc attgtcaaag tcgttgtctt ccaaaccaag
     7261 gtggacaatt tctttcttgt agcgaacaaa catcttcttg aaggcttgtt cactttcttc
     7321 gtcaatcttg aaccagagtt cttccattcc tggaaggctg tgaacgtagt ccatgtattt
     7381 ttgagttgtt tcatagtcac ctgaaactgt tccgttaatt ccctcgtcag cgactaggat
     7441 acggccttta aggccgattg atttacagaa agccaagtgg tctgcagcaa attgctctgc
     7501 attttcaatt ggagtataaa ggtagtaaag taagacacga atatcttttg ccataagatt
     7561 tgttctcttt tctattctta aattttctaa attttttatt tatccatact agtatacctg
     7621 cttgagaaaa cgaatgcaat ttatttgact ggaaattgta gagggttctc gaaatttcct
     7681 gaatggttaa aataaggaca agagaaaaca tggatatcta tatccttgtg ccaaaaaaac
     7741 cactgccctc cccagaccaa cctgaggaaa gcagtgattc ttattttagg agttaggaat
     7801 gaatacacga aatcaattta gctgattatt ttttgttttt caagaattca tcgtattgtt
     7861 tttgcatttc gttcaatact ttttcgtagg caccttcaga tttcaatttt tccatcaatt
     7921 ctggaatagc tttatctggg tctacagtac cagtgttgat agctgtatca aattgttgca
     7981 ttgtgttagc aatagctgag atttcagatt tcacattgtc agtattgaag ataaatccaa
     8041 gcgctggaga ttctttagct tctgccaatt ctttcttaga attttcgatt tgttggtctg
     8101 taacgttttc gttgatgtaa aggatccagt tgttaccagt gttccatcca cccatgtgag
     8161 tgtttccttt gtagccatca agaacgcgaa cacggttttc tttaccttca attttttccc
     8221 agttcttgcc ttctggaccg taaacaagac cgttcaagag ttctggattc gtattcaaga
     8281 ggttcaagat ttccattgat ttttctttgt tcttagagtt gtttgagatg acaaagttag
     8341 caacttgtgt tgtttggttt ttcttgatga agttagtaat tggtttgatt tggatatctt
     8401 tgttggcaac acgtgaaagc aagctgttac cgtagtcagc tggtcctact gtttcttcac
     8461 gaacgaacca agtatcttgt tgaaggtcaa aggaagtatc gcttgttgcg acgtcttttg
     8521 gaatgtagcc agcttcatag aatttgtgaa gagtcttcaa gtgttctttg aaacgaggca
     8581 cttcgtaacg gtttacaact ttagtagtat cgccttcaag gtcgataacg aatggaagac
     8641 cgtttgctac tgggtagtca aagttatcag atgggatgaa aactttacca atagcaaatg
     8701 gtactacgtc tggagctttt tctttaattt gtttcaagac tggctcaaga gtttcgtaag
     8761 aagtaacacc tgaaatatcg ataccatatt tagcaaggag agttccgttg aaggcaaagt
     8821 tttgagatga tgcaacgttg gctgcaactg gaacagcgta aatcttacca tttacagtat
     8881 tacccttgat gtaagctggg tcaagtgctt tgtaaaggtc tttaccttct tttttgtaca
     8941 attctgtcaa gtcagcgtaa gcaccttttt gagcatttac aatatagtta tctgcaaagg
     9001 caatatcata gttttcacca gatgatgtga taactgacat tttcttacca tagtcacccc
     9061 agccaaggta ttggatatcc aatttggcac caactttttc ttcaatgatt ttgttggcat
     9121 ttgctaacaa ttcatccaag ttgtctggtt tgtcaccgat ttggtacatt ttgataacag
     9181 gtttgtcacc tgaatcagca gcttttttgc tgttacctgt caaatttcca caagcagcaa
     9241 gacctgcagc cagagcgact acactagcag atgtaaaagc atattttttc cagtttttca
     9301 tgataaaaac tccttttttt atttttaaac ttataaacaa tgtaatgatc ttatactcaa
     9361 tgaaaatcaa agagcaaact agaaaactag ccgcaggctg ctcaaagcac tgctttgagg
     9421 ttgtagataa gactgacgaa gtcagttaca tatatctacg gcaaggcgac gttgacacgg
     9481 tttgaatttg attttcgaag agtattaact tcacacaagg gaagttggga actgagaaat
     9541 gttatttctc aataagcact attctttcac accaccgata gtcaaacctt ttacaaagta
     9601 gcgttggaaa aatggataca aaatcgcgat tggaagggtt gcaaccacaa ccatggccat
     9661 acgacctgtt tctttcggta gagcaactcc cagttgacca gtcaagccga ccgctttggc
     9721 aatgtagtcc atattttgtt ggatttgcat gagcaaatat tgcaatggat acaagttgtc
     9781 actcttgatg taaagaaggg cgttgaacca gtcattccag aaaccaagag ctgttaagag
     9841 cgtgatggtt gcgatacctg gtagtgacaa tggcaaacag atttggaaga aaatccgggc
     9901 ttcactggca ccatcgatac gagccgattc tagaatggct tctggaatgg tcttcttgaa
     9961 gaaggaacgc atcaagatga tgttaaatgg tgagagaagc attggaacaa tcaaggccca
    10021 aacagtatcc ccaagctgaa gcacacgagt caccatgata taacctggta ccaaaccagc
    10081 gttgaacaac atactgagca gaacgaagat ggtaaagaat ctgcgatact taaaggttgt
    10141 ccgtgaaata gcgtaggcat aggttgttgt gataaagaca tttgtcaatg tcccaactac
    10201 ggttacaaag acagagatga agagggcttg taggatttta tccttaaact gtgccaaaaa
    10261 ctcaaaaccg tctaagccaa attgggatgg gaagaagcta tagccgtatt ggaggaggct
    10321 tttctcgtct gtcactgaaa taatgataac gaatacaaaa ggtaggatac aagagagggc
    10381 aatcaaaccc gaaatgatac tgaagaagat atctgctttc ttactgaagg agtgaatgcc
    10441 gacattatca attttttctt ttttaatttt cttttttgcc atattctcct cctttctaga
    10501 acaaagctga gtttggatcg actcgtcttg caagcaagtt tgataggata accagaatca
    10561 aaccaacaac ggattggtaa agaccggctg ctgcagccat accgatatct gctgtctgag
    10621 tcaaaccatt aaaaacatat acgtccaaaa cgttggttac attgtaaagc tgaccagcat
    10681 tgtgtgggat ttgatagaag agaccgaagt ctgcgcggaa gatatttccg actgcaagga
    10741 tggtcaatac agttacaagc ggagtcaact gaggaatggt tacgttgcga atacgttgcc
    10801 acttgctagc tccgtccact gtcgctgctt cgtagtaggt tggatcaatt cccatgatcg
    10861 tcgcatagta catgacactg ctatatccaa aacctttcca aatacctagg aaaagtagga
    10921 gatagggcca gatgcccagg tcagcgtaga aattgacttc tttgagacca agactttcca
    10981 atagatgatt gaacacccct ttatcaatat ttaggaaggc atctgtaaag aaactgatga
    11041 taacccaaga caagaagtaa gggaacaaca tagaagtttg gaaaatcttc accattctct
    11101 tagaacggag ctcgctgagg ataatggcaa tccctacaga tacaactaaa cctagaaaga
    11161 taaagccaag attgtagagg acggtatttc gtgtgataat aaaggcgtct cttgaactaa
    11221 ataagaatct gaaattatcg agtccgaccc atttactatt tatgatacta tctatgaaac
    11281 cattactggt catgtggtag tctttgaagg caaccacgtt cccaaatact ggaatgtaaa
    11341 agaatagaat caaccagagt gcccctggca aaaccatcaa gagaaagatc cagttgtctc
    11401 tcaatgtttt tgaaaacttt ttcataattt cctccctttt tattttgata tccatctaaa
    11461 aattcttttt tagacttttg ataacgatta cattattagt atactcctat ttgcaggtta
    11521 ggttaaactc ctaattatag aaaaaactcc acaaattatg tagcagattt aaaactttat
    11581 caccactatc aaacaaatgt cctaaatcaa ttgtttattt tatctctatt agcccagtga
    11641 tggcgtcact ctgttataag catccaacaa cggggtatac tgaaaaatct ccagactagg
    11701 gaactcagcg atagttccta atctggagat ttttaatatg ttattaggcg tttgctttca
    11761 acttagcaat aacctcttta agattatcaa tcacctctgc ttcagtatgc tcagagcctt
    11821 tttcatctgc caagaacaaa actgcttttt gaagttcttt ttgagagttt tcaaggacat
    11881 ccttatctac tgtttcaagg tttgagtctt taagaagttt acttaattcc ttggctaatt
    11941 tcttgagttt gatttgcaga ctcatcttct cctgctgttt ctttgcccgc tgtttgtcct
    12001 ccatccttag ttgctgactg gctttcctta atggactcta gggaagcaat ggcatctttg
    12061 actgtttgca agatatcacg taaaccttgc tctgtcaaac tatcatctgc aaaagctttc
    12121 ttagcctctg ccaaaaccag acgtgctgaa tctgtggtag gattcgatac acctgtcaat
    12181 gatctcaaaa gattttctaa ggtttgagtc tgcttactaa tactagacta aaatcaaaaa
    12241 gcattatata acagtgatat gaaatcaact aaagaagaaa tccaaaccat caaaacactt
    12301 ttaaaagact ctcgtacagc taaatatcat aaacgccttc aaatcgttct atagtaaaat
    12361 gaaacaagaa caggacaaat cgatcaggaa agtcaaatcg atttctaaca atgttttaga
    12421 agtagaggtg tactattcta gtttcaatcc attatatttc gtctgatggg caaatcttat
    12481 aaagagatta tagagctttt atagtgattt gaaataagat gtgaacaact ctatcaggaa
    12541 agtcaaatta atttatagaa atattttagc agccaaggtg tactgttata gattcaatac
    12601 act
//
LOCUS       DAVLYV010000051        12481 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLYV010000051 DAVLYV010000000
VERSION     DAVLYV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12481
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.051"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            479..772
                     /locus_tag="VYO70_001435"
                     /pseudo
     CDS             479..772
                     /locus_tag="VYO70_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            879..1230
                     /locus_tag="VYO70_001436"
                     /pseudo
     CDS             879..1230
                     /locus_tag="VYO70_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1506..2812
                     /locus_tag="VYO70_001437"
                     /pseudo
     CDS             1506..2812
                     /locus_tag="VYO70_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2852..3862)
                     /gene="tsaD"
                     /locus_tag="VYO70_001438"
     CDS             complement(2852..3862)
                     /gene="tsaD"
                     /locus_tag="VYO70_001438"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1713654.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3852..4289)
                     /gene="rimI"
                     /locus_tag="VYO70_001439"
     CDS             complement(3852..4289)
                     /gene="rimI"
                     /locus_tag="VYO70_001439"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1713655.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVIAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(4286..4969)
                     /gene="tsaB"
                     /locus_tag="VYO70_001440"
     CDS             complement(4286..4969)
                     /gene="tsaB"
                     /locus_tag="VYO70_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1713656.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKNLDRIVVAEGPGSYTGLRIAVATAKTLAHTLNIELVGMSSLLALVPY
                     QQEGLFVSLMDARRNNVYVGFYENAKPVMPEAHLPFERVIELIKGASQVTFVGEVGPF
                     VEQIQEYLPRTNFKETLPNAANLALWAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            5147..5332
                     /gene="cibA"
                     /locus_tag="VYO70_001441"
     CDS             5147..5332
                     /gene="cibA"
                     /locus_tag="VYO70_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HEV1713657.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            5334..5486
                     /gene="cibB"
                     /locus_tag="VYO70_001442"
     CDS             5334..5486
                     /gene="cibB"
                     /locus_tag="VYO70_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1713658.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(5699..5920)
                     /locus_tag="VYO70_001443"
     CDS             complement(5699..5920)
                     /locus_tag="VYO70_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713659.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            6302..6535
                     /locus_tag="VYO70_001444"
     CDS             6302..6535
                     /locus_tag="VYO70_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1713660.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6537..8216
                     /locus_tag="VYO70_001445"
     CDS             6537..8216
                     /locus_tag="VYO70_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1713661.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQGEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8314..8391)
                     /locus_tag="VYO70_001446"
                     /pseudo
     CDS             complement(8314..8391)
                     /locus_tag="VYO70_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(8595..10508)
                     /gene="mnmG"
                     /locus_tag="VYO70_001447"
     CDS             complement(8595..10508)
                     /gene="mnmG"
                     /locus_tag="VYO70_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1713662.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10518..10973)
                     /locus_tag="VYO70_001448"
     CDS             complement(10518..10973)
                     /locus_tag="VYO70_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1713663.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWKGQLRHIHLEK"
     gene            complement(11114..12235)
                     /gene="mnmA"
                     /locus_tag="VYO70_001449"
     CDS             complement(11114..12235)
                     /gene="mnmA"
                     /locus_tag="VYO70_001449"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1713664.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3539 a         2925 c         2465 g         3552 t
ORIGIN      
        1 atatctaaat ttttataatc atcaataacc tttgaatctc tagatacgat tatcaaaata
       61 ggatatagaa acatccttct gatctgcttt ttacaaagtc caattatatg cggatctata
      121 cctccacaat gtccattatt atacctaact ataatatgag ccgaaaacac tatatcctta
      181 atgtctccat atccatcagg gatattaata tttatttttc cacaactata ttgcattgta
      241 accatctcct taaacgacgc attatgatat ttgatagaga aatttttatg aataactcaa
      301 taattttata gtaaatcatg cttatatctc aaagatacct attttctctt gtctcgacct
      361 tctccaaaga attgctataa tactattaca aatacatctg cactacactt caaattttag
      421 cactgtataa aaacgtttca acacactaac tttaagaaaa cttccactat taattgaaaa
      481 agttgataga gataaattaa agatctatat tgaaactcat cccgatgctt atttgactga
      541 aatagctgct gaattcaact gtcctccaac aactattcat tacgctctaa aggctatggg
      601 atatagtcta aaaaagagcc gtacctactg cgaacaagac ccagaaaaag taaatcggtt
      661 ccttaaagaa ttgaatcact taagctacct gactcctatt tatatttatg agacaggggt
      721 tgagacctat ttttatctcg aatatgatcg agccttgagc aggcagttag tctctctgga
      781 agaagatata attatttgaa ttaagatcga gacaacgcac accagagatt gcgatactgt
      841 tatagaagta ctaatgccct tttttgtttc aatatactat ggctccgatg acctataaag
      901 atacgatgac gagtgacttt ttcgaagctt gcttccaaaa attcttacta cctactttag
      961 atacaccatc ccttatcatt atggacaatg caaggtttca cagaatgaac atgtgtaagg
     1021 agcagggcat agactgctac cacttcctac ctattcaccc gagtataatc ccattgagaa
     1081 aatatgggct tacatcaaaa acatctcaga ataatattgt caaattacga tgcttttctt
     1141 gaggtacttt tgtcctattc ttgtttcagc cgactatact ccgttattgg gcagctacgg
     1201 aacagtcgat gggacgatgg ggggacataa aaaaatcctc cagttttgtt ttttataaca
     1261 gtatactgga gaattgacaa tctcggtaga tacctcgtta tagcgcggtt acttattagg
     1321 cagttacaaa acaactgtga acagaaaaca ttccagagtc agacaatact ttggaatgtt
     1381 ttggctctat aatttctgta gtgggtaatc ccaccccagg aattataggg tcgtttcttg
     1441 tagaaaaaaa gccccatatg acctataatg aaaagcgtct aaccaactca ttagaaaggg
     1501 gtcatatgga acaacttaag aataccacag atttgctcgg attggaagac aaaaatatca
     1561 aaatcttgtc tgttctgaaa taccaaaccc atctagtcgt tcaggcaaag ttggattccc
     1621 ccgctcctcc ttgtcctcat tgtcaaggga agatgatcaa atacgacttc cagaaagcct
     1681 ctaaaattcc gcttctcgac tgtcagggtt tacccacggt actgcatctc aaaaagcgcc
     1741 gctttcagtg caagaattgc cttaaggtgg tcgtttctca aacatccatt gtcaagaaaa
     1801 attgccagat ttccaacatg gtgagacaaa aaatcgctca gctcctcctt gaaaagcagt
     1861 ctatgactga gattgcccac agattggcgg tctcaacttc caccgtcatc cgaaaactga
     1921 gggaatttaa gtttgaaacc gattggacca agttgccaaa agttatgagt tgggatgagt
     1981 atagcttcaa aaagagcaaa atgagcttca ttgcccaaga ttttgagtcc aaatccatcc
     2041 tcgcaatttt agacgggcga actcatgcgg tgattcgaaa ccatttccaa cgctatcaga
     2101 gagaggttcg ggagctggtc gaggtcatca ccatggacat gtacagccct tattatcggc
     2161 tcgctaagca actctttcca aaggcgaaga ttgttcttga ccgcttccac attgtccaac
     2221 atctgagccg agctatgaac cgagtacgaa tccaaatcat gaaccaattt gaccgaaaat
     2281 ccttggagta tcgggcgctc aagcgctttt ggaaccctcg ctttttcgtt tctaggctcg
     2341 ggctaaatca gtccactgga ctgatttact acaccagtat agcttcaagc tctgtcagaa
     2401 acgattctat cagcccacgt ttcgaatgca cttaacccat cgggaagtac gagataagct
     2461 gctgtcttac tctgagggat tacaggttca ctacgaactc tatcaactcc tgctctttca
     2521 ttttcaagag aagaatgccg accatttctt tggattgatt gagcaagaac tgccaacggt
     2581 tcatccgctt tttcaaacgg tcttttggac ttttttaagg gatagagata agattatcaa
     2641 cgcacttaag ctgccttatt ccaacgctaa acttgaagcg accaataatt tgattaagat
     2701 tatcaagcgc aaagcctttg gtttccggaa ctttaacaat tttaaaaaac ggattttgat
     2761 gactttgaac atcaaaaaag agagtacgaa tttcgtactc tccagattgt agcttttcgc
     2821 ctacccacta cacttgacaa agagccactc tttattccat ggtatcaaag gcaagacttg
     2881 gtttggcatt gaggtcccag cctgcgaagt tttctttgtt ccactcgctg acgctggcat
     2941 aggcaatcat acctgcattg tctccgcaga gtcgcagagg ggggatgata accttgacat
     3001 ctgtgatttc ggctgctagg cgttctctga gacctttatt ggctgccaca ccacctgcca
     3061 caaccagggt tttaacagga tatttctcca aagccttctt ggtttttgcc atgagaatgt
     3121 ccataactgc tgcttggaag gaagcacaca aatcttctgt agacaggctt tctccctttt
     3181 gctcggcatt gtgataaaga ttgataaagg cagatttcaa acctgagaag gagaactcca
     3241 gattatcttc cttaatcatg gcacggggga aatcataaat atcctgcccc tgatgagcca
     3301 gctcgtcaat ctcacgacct gcaggatagg tcaagcccat gacacggccg accttatcat
     3361 aagcctcacc aaccgcatca tcacgggttt ccccaacaat cttataatct cctgcctccg
     3421 aaacataaac caactctgtg tgtccgccgc tgaccaagag ggctagcaag ggaaactcca
     3481 aaggctccac actctgagct gccatgaggt gcccagccat gtgattaaca ggaatcagtg
     3541 gaagtccgtg agcccaagca aaggccttgg cagctgacaa accaactagc aaggctccga
     3601 ccaagcctgg tccgtaggta accgcaacag ctgtcacgtc ctcttcggta atccctgctt
     3661 ctgccaatgc ctcctcgata caggctgtaa tgacctcgac atggtgacga ctggctactt
     3721 cgggcactac gccaccaaaa cgtttgtgac tctcaatttg actagcaatg acattggaca
     3781 agagctcatc gtcgtttttc aagacggcga cactggtctc atcacaggat gtctcaaatg
     3841 ctaaaatata tctatccttc atctatttct ctcttcatga taatggcgtc ctcgactggg
     3901 tcatggtagt aggcctttcg ctcagcgatg accgccattt tttctttctt gtaaaatgct
     3961 tgcgctcgtt gatttgactt tctgacttcg aggaaaatct ccttatctgt cggcaattga
     4021 gcaaacaagg ctgacgcaat tccctgaccc tgataggctc ctttgacagc gatttgcagg
     4081 acttctgctt caaaaagatt ctcctgcaca gctagaaatc caatcacttc tgccccatca
     4141 taagccaatg cataccaagt ctggtcttgg gacagatctg cttggatttg ctccagagtc
     4201 caaggactga ctaggtaaac agctgctata acagcgtaga tggcttgagc taggtcaggc
     4261 tgttgttgaa ttcgcttgat ttctatcata ggcgtttaat gtaggactca ccagactcgg
     4321 tatggttctt gagccagttt tcctcagcct cgactcgttt gaggtaattc ggcacaaaat
     4381 catgcaagga gtctgcttcc ttgtcccagg cccatagagc tagattagct gcatttggca
     4441 aggtttcttt gaaattagtc cttggcaagt attcttgaat ctgctcaaca aaggggccaa
     4501 cttctccgac aaaggtaacc tgactagcac ccttgattaa ctcaatcact cgctcaaagg
     4561 gtaagtgagc ttctggcatg acgggtttag cattttcata aaatcctaca taaacattgt
     4621 tgcgacgcgc atccatcaaa gagacgaaca agccttcttg ttggtagggt accagagcca
     4681 agaggctaga cataccaact aactcgatgt tcagagtatg agccaaagtc ttggcagtgg
     4741 ctaccgcaat tcgcaagcct gtgtagctac ccggcccttc agctaccacg attcggtcca
     4801 aattcttggg tgtccaatcc aaacttgcca tcaaaaaatc tatggcaggc ataagagtaa
     4861 tactgtgatt tttcttaata ttaatcgtcg tctcggcaag aacctgcttg tcctctaaaa
     4921 tagcgagaga aagagccttg ctggacgtat caaaagctaa tactttcata acacattcct
     4981 atctttttgt ctgcttacta ttatactaca aaagctggca catgggaatt ttctttgccc
     5041 ccagacaaga gtgccctcac ttaactaaaa ataatttaaa aaaatgctca cttttccttt
     5101 tcttttccga atataaaagt gaacaagaaa aaaggaggaa agttcaatga caaattttga
     5161 cattcttgac aatcaatttt tatccttatc tgaaaatgaa ttatcagata ttgatggcgg
     5221 tctcgctccc ttggttatct ttggagtagc agtatcttgg aaagctattg caggtggaac
     5281 agcacttata ggttctggtt tggcagctgg ttatttttta ggaggagatt aatatgatga
     5341 aagatttgaa caactgtcgt gaaatttcta ataaggaatt gcaagaaatc aagggtggct
     5401 ttggtgtagg tgttggtatc gctttattta tggcaggtta taccattgga aaagaccttc
     5461 gtaaaaagtt tggtaagtca tgctagataa gaaacacatt tttagaagga taaattttat
     5521 tgtcttcatc tcttacagtt tgctcagcat tctcaatgat ttgaacatta ctaccatccc
     5581 tttaccattc gatttatctg tttgtattgt tttattttta tgcttcaact ctatttttga
     5641 gcagaacaat gactcccata aaaataataa gctttgaaaa ttccattgtc atatcatttt
     5701 agaaaaatgc aaagaccacc tcatcttgat agatggggtg gaattttcgt gtcgtaaatc
     5761 tactatctct acatccccaa acaaaaaacc ccagcataag cagggcatct aagcatttaa
     5821 tccaaagtaa aatacaaacc aaacgacata ggtcacgagg aggagaaaaa gcgagtagag
     5881 agtcacaaag gtaattttcc tcaagaactt ggtttgtcat cgttccagtt tggcaaatag
     5941 aagatttcct ccataaacgc aaaacaaaaa cgataaaagc tagcaaacga actccgatag
     6001 caaaagcaaa taagttatac atagggcaac ctccttgact taaaatctat atggaattat
     6061 gacaagcaat aaatttcact tccgttatca acataataca ttttctttat tttcgcaaac
     6121 gcttaccgaa gaaatcgtcc cctaactttc tcgtttccgt cttttactaa tttttcattt
     6181 tgtggtataa ttgaaataat tgtaacgaat caaggtcaat ctagacacaa aatggaatga
     6241 aatcaagcaa atatctgcta aaagtttgga ataagctgac ctgtaaatag aaaggaacta
     6301 tatgatttac aaagtttttt atcaagaaac aaaagaacgt agcccacgcc gtgaaacaac
     6361 acgcgcgctt tacctagaca tcgatgccag ctcagaactt gagggccgta tcactgctcg
     6421 ccaacttgtc gaagaaaatc gcccagagta caatatcgaa tatatcgaac tcttgtctga
     6481 caaattgctc gattacgaaa aagaaactgg cgccttcgaa attacggagt tctaatatgg
     6541 cctacactct taaacctgaa gaagtcggcg tttttgccat cggtggtcta ggagaaatcg
     6601 ggaaaaacac ttacggaatt gaataccaag gcgagattat catcgtcgat gctgggatta
     6661 aattcccaga agatgacttg cttggtatcg actatgtcat tcctgactac tcttacatcg
     6721 tggacaatat cgaccgcgtc aaggctgttt taatcacaca cggacacgag gaccacattg
     6781 gtgggattcc gttcctactc aagcaagcaa atgtccctat ctacgctgga ccgcttgcct
     6841 tggctttgat ccgtgggaaa ctcgaagaac acggcctctt gcgcaacgcc aaactttacg
     6901 aaatcaacca caacaccgag ttgaccttta aaaatctcaa ggcaactttc tttagaacga
     6961 ctcactctat tccagagcct ttggggattg tcattcatac tcctcaaggg aaaatcgtct
     7021 gtacgggtga ctttaagttc gactttactc cagttggaga acctgcggac ttgcatcgta
     7081 tggctgcgct tggtgaagaa ggcgtgctct gtctcctgtc tgactcgaca aatgcggaag
     7141 taccaacctt taccaactct gaaaaagtcg ttggtcagtc cattatgaag attatccaag
     7201 gtattgaagg acgtatcatc tttgcatcct ttgcctcaaa tatcttccgt ctccagcagg
     7261 caacagaagc tgctgttaag actggacgca agattgcggt ctttggtcgt tctatggaaa
     7321 aggccattgt caacggaatc gatcttggct acatcaaagc tcctaaggga acctttatcg
     7381 agccaaatga aatcaaagat tatcctgcag gagaagttct tatcctctgt acaggtagtc
     7441 agggtgagcc tatggcagcc ctctctcgta tcgccaacgg aacccaccgt caagtacaat
     7501 tacaaccagg tgataccgtt atcttctctt ctagtcccat ccctggaaac actactagcg
     7561 tcaacaagct gattaacatc atttctgaag ctggtgtcga agttatccac ggtaaagtga
     7621 acaatatcca tacatctgga cacggtggtc agcaagagca aaaactcatg ctctgcttga
     7681 ttaagccaaa atacttcatg cctgtccacg gtgaataccg catgcaaaaa gtccacgctg
     7741 gactagcagt ggatactggt gttgagaagg acaatatctt tatcatgagc aatggcgatg
     7801 tgcttgccct tactgctgac tcagctcgta tcgcaggtca tttcaacgcc caagatatct
     7861 atgtcgatgg aaatcgtatc ggtgaaattg gcgcagctgt cctcaaagat cgtcgcgatc
     7921 tatctgaaga cggtgtcgtt ctggcagtcg caactgttga cttcaaatcg cagatgattc
     7981 tatctggtcc agacatcctc agccgaggct ttgtctacat gagagagtct ggcgacttga
     8041 ttcgccaaag ccagcgtatc ctcttcaatg ccattcgtat cgcactgaaa aataaggatg
     8101 ctagcgtgca atctgtcaat ggtgccattg tcaacgctat tcgccccttc ctctatgaaa
     8161 ataccgaacg tgaaccgatc atcatcccga tgatcctcac accagatgaa gaataatact
     8221 cttcaaacca cgtcagcttc gccttgccgt atatatgtta ctgacttcgt caattctatc
     8281 cacaacctca aaactgtgtt ttgagctgac ttcgtcagtt ctatctacaa cctcaaagca
     8341 gtgctttgag caacctacgg ctagcttcct agtttgctct ttgattttca ttgagtacaa
     8401 gtcaacaaaa cagccccgtc ttcggagctg tttttctcta tgctttcttt tgagattaaa
     8461 actcaatgaa aatcagacca gaaggctagg aaaatgcata acttgagtta gctcccatag
     8521 ttcgggaaac tatgggaggc tggagatgaa tcaaagccaa gctttgaact cattcgtaag
     8581 aagccaacga cgtatcattt tgatttttga agagttttag aaatactacg atttttacct
     8641 tccagataca ccatcaaaat agaaatatct gctgggttta ctcccgaaat acggctggct
     8701 tggccgatgg tttctggatt gatgagtttg aacttctgac gagcttctgt tgcgatagag
     8761 tcaatgtcat cccagtcaat attggctgga atgcgttttt cttccatacg tttcatcttg
     8821 gcaacctgat ccatggcttt tgaaatatag ccttcgtact tgatttctgt ttcaatcaat
     8881 tcgataatct tgtcatccaa gtcttctgca gctggtccga tgaaggccac cacatcttgg
     8941 taagaaactt ctggacggcg aaggaattcc ttggctgtca ccgcatctgt caagggcttg
     9001 aagcccattt cctcaacctt agcattggtt tccttgactg gcttgagttt gatactgtct
     9061 aggcgtttca tctcattatc aaattgattt ttcttgattt caaaacgagc ccagcgttca
     9121 tcatccacaa ggccaatctc gcgtcccatc tcagtcaagc gcatatcagc attgtcatgg
     9181 cgaagaatga gacgatattc agcacgactg gtcaagagac ggtaaggttc aatggttccc
     9241 ttggtcacca agtcgtcgat catcaccccg atataaccgt cacttcgctt caaaatcaac
     9301 tcaggcttac cttggatttt cagagccgca ttgatacccg cgataatccc ttggcctgct
     9361 gcctcttcgt aacctgacgt tccatttgtc tgaccagcag tgaagagacc tgagattttc
     9421 ttggtttcca gagtcgcacg caactgatga ggcaagacca tatcatactc aatagcataa
     9481 cctgtccgca tcatctctgc attttccaaa cctttgatgg aatgaaccaa gtcacgctgg
     9541 acatcctcag gcagactggt tgaaagtcct tgcacataga cttcctcagt attgcgccct
     9601 tctggttcaa ggaagagttg gtgacgttcc ttgtccgcaa agcgcacaat cttgtcttca
     9661 atcgacggac agtaacgagg ccccactccc ttgaccacac ctgtaaacat aggcgcacgg
     9721 tggaggttgt tttggataat ctcatgactg gtaccattgg tgtaggtcaa ccagcatggc
     9781 acttggtcct tgacataatc ctcatcacgt gaagtgtatg agaagtgatt aggcgcttca
     9841 tctcctggtt ggatttctgt cacgtcgtag ttgatagaag aagccttgac acgtggaggg
     9901 gttcctgtct tgaaacgacc gatttcgagg cccagttcct tgagattatc agctaggtta
     9961 atagaagcca agctgtggtt aggacctgat gagtacttga ggtctccgat gataatttcc
    10021 ccacggagag cagtccctgt cgtcacaata acagccttag cagcatattc ttgatgggtg
    10081 gctgtacgca caccgacaac cttgccatct tccaccaaaa tctcatcaat catggtttga
    10141 cgaagggtca gattttcttg gttttcaact gtcttgcgca tttccttaga gtaaagttcc
    10201 ttgtcagcct gcgcacgaag ggcacgaacg gctggcccct tccctgtgtt gagcatcttc
    10261 atctggatgt aagtcttgtc aatggtcttg gccatctcgc caccgagggc atcgacttca
    10321 cgtacgacaa tccccttagc agaaccaccg atagagggat tacaaggcat gaaagccagc
    10381 atttcaatat tgatggtcgc aagcaggacc ttacagccca tacggctagc ggccaaggaa
    10441 gcctcaaccc cagcgtgtcc cgcaccaatt acaataatat cgtattcttc agtaaaatga
    10501 tatgtcatat ttctctccta tttctcaaga tggatgtgtc ttagttggcc tttccaatct
    10561 ggtagggccg tttttaaaaa ggctggaact agctggatat cctcaagctt atccaagtca
    10621 atccactcac agggctgcct tttctcatct tcctgcatgg tcaatggggc atcctccagt
    10681 aagtctacca gataatgaaa ttcgatattg tgataggaaa caccgtcctg ttcaaaacga
    10741 ttttcaacca caaaagctag ctgcccagct tgagctttga cacccagttc ttccttcact
    10801 tcacggacta ccgcgtcttc cgtgctttca ttgacttgaa tcgcacctcc aatagtgtaa
    10861 tacttgccct tgtctttggt aactagaagc ttgtgatttt ggacaatcaa ggctgtagcc
    10921 cgaacaccaa aaaccgtatt gtctactttt gtccgaaagt cttgttgagt cattcttgtc
    10981 ctttccctta aacgacacaa aaacagtcaa aactacaaag aagtgcagga caaaaaagcc
    11041 tgcaacatcc atgagctttg accatcattt ctattgctgt tattattgta gcaaattgag
    11101 aaaatttgtc aatctaaatg tactgacaaa cttgtccatc gcggtaagca ttgtcaatca
    11161 aaccgccacc tagacactct tcgccatcgt aaaagacaac tgcctgtcct ggtgtaatcg
    11221 cgcgttgtgg ttccgcaaag ataacttctg ccttgtctcc tttgacatgg acggtcacct
    11281 tagagtcagg ctgacggtaa cggaatttag ccgtacattc tagcgtaaac tcttctggca
    11341 tttcacgagt aaagtggact tgactggctt ctaggctagt tgacatgagc gaatcatggt
    11401 agaatccttg tcctacatag agaatattct tgcttagatc ttttccaaca acgaaccaag
    11461 gggcattgtc accgccgtgt tgcccaccga taccgagtcc gccacgctga ccgattgtat
    11521 agtacatcag accagcatgc tcgcccatat cgcgaccatc cacagtcatc atacgaccag
    11581 gctgagctgg caggtagttg ctgagaaagt ttttaaagtt cttttctccg ataaagcaaa
    11641 tccctgtcga gtctttcttc ttagcagtcg aaaggcctgc ttcttctgct agtctgcgta
    11701 cttcaggctt ttccaaatgt cccagaggga acatggtttt ttggagttgt tcttgcgaaa
    11761 gttggctgag gaaataggtc tggtccttgc cattgtccac gccacgaagc atgtgaacag
    11821 tgccgtcctc atcacgcgcc actcgagcat aatgcccagt cgctacatag tctgccccca
    11881 aggtcatggc atagtccaaa aaggccttga acttgatttc cttgttgcac ataacgtccg
    11941 gatttggcgt gcgccctgca cggtattccg ctaggaaata ctcaaaaacg cggtcccagt
    12001 actctttttc aaaattgaca gagtagtagg gaatgccaat ctggtctgcc accgcaacca
    12061 catccttgta atcttcggtc gccgtacaaa cgccgttttc atctgtgtca tcccagttct
    12121 tcatgaagat accgatcaca tcgtagccct gctccttaag caaaagagcc gtcaccgacg
    12181 aatcaacacc accactcatc cccacgacaa cacgtgtttt agagttatca ctcatggtaa
    12241 gtctcccatc tattcgttta ttcacgattg aaggtcgtgt gtgcttcacg attgaaggtc
    12301 gcttgagcag tattcattat aacacgcttg gttagagaag acaaggaaga gcttaaaaat
    12361 tgttttttaa aacttatagt caatatacag attatgtgaa tgatgtgagt ataccttctt
    12421 cttatcttca ttcaaataac tataataaat aaaacaaccc attttctcta atctattttt
    12481 c
//
LOCUS       DAVLYV010000052        12406 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLYV010000052 DAVLYV010000000
VERSION     DAVLYV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.052"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(90..851)
                     /locus_tag="VYO70_001450"
     CDS             complement(90..851)
                     /locus_tag="VYO70_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1713665.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMVHKK"
     gene            complement(893..1519)
                     /locus_tag="VYO70_001451"
     CDS             complement(893..1519)
                     /locus_tag="VYO70_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1713666.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLDVERKGKVARVFLKDNGE
                     TVAWNIFEVSSLFETAERGQVFLTDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKTVQIVAGAPNARVGLKTIVALPGAMMPKGNLIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDSAKHWTA"
     gene            complement(1535..1852)
                     /locus_tag="VYO70_001452"
     CDS             complement(1535..1852)
                     /locus_tag="VYO70_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1713667.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKQQINDFLA
                     GLK"
     gene            complement(1849..2148)
                     /locus_tag="VYO70_001453"
     CDS             complement(1849..2148)
                     /locus_tag="VYO70_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1713668.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(2433..2624)
                     /locus_tag="VYO70_001454"
     CDS             complement(2433..2624)
                     /locus_tag="VYO70_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HEV1713669.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(2785..3459)
                     /locus_tag="VYO70_001455"
     CDS             complement(2785..3459)
                     /locus_tag="VYO70_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713670.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     ELVYVK"
     gene            complement(3465..3911)
                     /locus_tag="VYO70_001456"
     CDS             complement(3465..3911)
                     /locus_tag="VYO70_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1713671.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(4025..4528)
                     /locus_tag="VYO70_001457"
     CDS             complement(4025..4528)
                     /locus_tag="VYO70_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1713672.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(4525..4887)
                     /locus_tag="VYO70_001458"
     CDS             complement(4525..4887)
                     /locus_tag="VYO70_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1713673.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(4953..6818)
                     /locus_tag="VYO70_001459"
                     /pseudo
     CDS             complement(4953..6818)
                     /locus_tag="VYO70_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(6828..7298)
                     /locus_tag="VYO70_001460"
     CDS             complement(6828..7298)
                     /locus_tag="VYO70_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1713674.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(7422..8138)
                     /locus_tag="VYO70_001461"
     CDS             complement(7422..8138)
                     /locus_tag="VYO70_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1713675.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(9019..10434)
                     /gene="ply"
                     /locus_tag="VYO70_001462"
     CDS             complement(9019..10434)
                     /gene="ply"
                     /locus_tag="VYO70_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1713676.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALMKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(10445..10777)
                     /locus_tag="VYO70_001463"
     CDS             complement(10445..10777)
                     /locus_tag="VYO70_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713677.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(10848..11456)
                     /locus_tag="VYO70_001464"
     CDS             complement(10848..11456)
                     /locus_tag="VYO70_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713678.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(11469..11927)
                     /locus_tag="VYO70_001465"
     CDS             complement(11469..11927)
                     /locus_tag="VYO70_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1713679.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            12052..12406
                     /locus_tag="VYO70_001466"
                     /pseudo
     CDS             12052..12406
                     /locus_tag="VYO70_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3697 a         2859 c         2224 g         3626 t
ORIGIN      
        1 tttattatac atattttctt cctcctactt atctattcgt aggaaatcaa aaaaagttac
       61 agaaatttgt aacttttcga gaaaattttt tattttttat gaaccatgaa acctgtcgcc
      121 tgttgattgg ccataatggt catatctgta atctgaacac gacgaggttg actagtcaca
      181 tagactactg tatctgcaat atcctgagct tgcaaagctt ctattccttg gtaaacggac
      241 gcagctcgtt ctttatcacc atgaaaacgc actgtagaaa aatctgtttc gacaattcca
      301 ggctgaatgg ttgtcacctt gatatccgtt gcgatggtat caattcgcag tccatccgaa
      361 aaggtcttaa ccgcagcctt ggtagctgag taaacagcgg caccagcata ggcgtagatt
      421 cctgcggttg accccatatt gataatatga ccttgattga cttttatcat tgctggcaag
      481 aaacagcgag taaccgccat cagaccttta acattggtat ccaacatggt caacatatcc
      541 aactcctcat agtcttgata gggagccaag tcaagagcca gtccggcgtt gttgaccaag
      601 atatcaatcc tccctatcgt ttctagaata tcagagcaaa cagtcttcac catgaccata
      661 tccgtcacat ctagtggaaa agtccagacg gtttgatttg gaaagcttac tgcaaactcc
      721 gacttgagga tttctaatct gtctatccgt cgtcctgtta gaacgacatc ctcaccctgc
      781 tccagataag cacgcgcaat cgcttcaccg attcctgagg ttgctcccgt aatcacaaca
      841 ttttttgcca tcttatttcc ttctagctgg tctatcagat attaacaact tcttaggcag
      901 tccagtgttt agctgagtca aatggtgttc cgacaacttg gtcttctgat aattcaagca
      961 ccccacgttt ttgtggagca tttggcagat gcaattcacg aggactgcac atcataccaa
     1021 aactcttttc accacgaagt tcacctggga aaatgagatt cccttttggc atcatagctc
     1081 caggaagcgc gacaatggtt ttcaacccca cacgcgcatt gggagctcct gcaacgattt
     1141 gtacagtctt atcacttgcg actgcaactt ggcagatgtt gaggtggtca ctatctggat
     1201 gggctaccat ctcaacaatt tcaccgacaa caaacttagg ttccttatca ttaacaattt
     1261 cttctgtaaa accttccgcc tgcaactctt ggttcaaacg agcgacttgc tcatctgtca
     1321 aaaagacttg accgcgctct gcagtttcaa ataaacttga aacttcgaaa atattccaag
     1381 ccactgtttc cccattatct ttgagaaaaa cacgggctac cttgcctttg cgctccacat
     1441 ccagtttggc atctccgcta tttttcacga tgaccataag gacatcaccg acatgttctt
     1501 tattatatgt aaaaatcatt gtttcctttt tctcctattt cagtcctgct aaaaagtcat
     1561 tgatttgttg cttgctttta cggtcgcgat tgacaaaacg accgatttcc ttgtcctttt
     1621 ctagaacaac aaggctagga attccgtaaa catcccagag tttggccaaa tccatatact
     1681 gatctcggtc cattcgaata aaggtgaact ctggattggt ctcctcaatc tctggtaagg
     1741 caggataaat ataacggcaa tcgccacacc agtctgccac aaaaaggaag accttcttgc
     1801 ccgctttttc cactaaagat gctaattctt ctaaacttgc tggctgtatc ataagacttc
     1861 ctcctcatag actaggtctt cattttcata gacaaaggta taatgacggc catcctcaaa
     1921 aatgacgcca ccaaccaagc tctccagact gctttcgtaa acttgaacat agagggtcgc
     1981 aatttccccc atgtcggaaa aatggtctcg cacaatctct gtcaactctt cctgagtctt
     2041 catgagctta cggtcatctg caactttttt cgtagcaaga gcaaggcttc caatacctag
     2101 cagagccaag cctgccatcc acattttttt agctttcata ccattcattt taacacaaaa
     2161 aaggcttcag gacaaatgag gaagcagcag aaaagcaagt aaaaagcctc ttcctttaag
     2221 gaaaaggact tcgtatacta agagcaaact aggaagctag ccgcaggctg ctcaaaacac
     2281 ggttttgagg ttgtagataa ggcgacgctg acgtggtttg aagagatttt caaagagtat
     2341 tattcttatt gccaggcacc taagttgcca acgtagtaac catcaggtgt gtaggtattg
     2401 cgaaacatct tacttgatga agccagataa tactacttgc cattgtcttt gacccaatca
     2461 ttcgcaatca tggaaccaga agaacttaca taataccatt ctcccttgtc ataaacccaa
     2521 gtactgactt tcatggttcc tgagcaatta aaggcaaaaa aactgtccaa tatcattcgt
     2581 tttttaaaag catttgacac tacatcatct acgctgtagt acatagatac atcaaacgta
     2641 aaagccaaaa tatcgaaaaa tatacaactt ttaaaggcag catttcattt tttaaagtct
     2701 aacaaccata gaactgaaat cagagcaagg catcaatttc ctactcttag tacgtctatt
     2761 ttgatggttg aagtgaagat ttttttattt aacgtagaca agctccaact tcccaaaggc
     2821 cacgtctcca cgaataatca aggttttgct tgttggcgct acaggagcgt ccgccttggc
     2881 tgcaccaaag gatgtttcta ctttcaaatc gacacgccag tgttgtggaa catagacggt
     2941 tgcatttccg aaggccactt caatatttaa agttgcaaaa tcacctagca tctctgcatt
     3001 atcatagtag attttagcat ccccaaaagc gacttccact tggtcatcta cgagatcttg
     3061 atcttgctta tagaaggtcc cgctaccaaa agcgacttcc ttatccgtga cgactgtttt
     3121 tttcccattg taccaccatt ttttttcatt ccagaactta cttgaatgcg tcagataacc
     3181 aacaccaagt accaccaaga tgctagccca aataagagaa tgattggtaa ctggtaacaa
     3241 gtcataagcg taatttgcaa tgatgagcgc cagtaaacct gtaaaaactg ccgaagtgag
     3301 atgacgtcta aggatggact caatggactt ataagcaaaa aaaacaatac ctagtaaagg
     3361 ccatatttta ccatccaaag aaggaattcc aaaattccct tgcagcaaga tccaagctgc
     3421 taaaaccaat aaaacaatac caaatgcttt ctttttcatg ttttttacct catgtttctt
     3481 agattttctt ttaggaggga ttggtaatgc cgtgagacat gaacctcctt gtgcgtctga
     3541 taaaaggaaa tggtgcccgt tcctgaaaag gacttgtcca ctgagtaaat ctgacggatg
     3601 tttgcgatcg ttgacttgga aactcgatta aaatagcgag gcaagataga ctccaactca
     3661 tagagcttga ggcgaacctc ataagcttcc ttctggttat gagcgtagat cttgctccct
     3721 tctgtttcaa agaagagaat ttctgacagg tctagataat attcacctgt ccccttgtaa
     3781 aaagtcaacc tcggagcctt tgactcttgc aagagccgtt gcaagtctgc aatttcatct
     3841 gtcaaagtgg gggtcttgat gacaatttca gtctcctcta aattgccgtc aatctcaatt
     3901 cgtaacttca tcacatctcc tttctgactc tactatatca aagctaaatt aggaatacaa
     3961 gggcaaaaaa gcaagtggtc aatttgaagc agtaagtggt tgtaggacta gcttaactta
     4021 catgtcagaa taggaagaaa aggaatcccc acaccaaacc acaagcatag ccaaccaaga
     4081 catccttggg ataatgaaca ccccctaaaa ccctcaccaa ccccagcaaa gctgaaaaga
     4141 tcaagcatac taagccaata ggtagactag catgcagaca ggccatggag atgatagttg
     4201 ccgaaaagac atggcgactg ggcatcgacc gtcccgaact atccttatca agaaggggac
     4261 agatatccca agtttcataa ggtctcgggt gattgatttt cttacgaaac agggacaagg
     4321 ccacaaaacc tgatgcagga ataagcacat aaaccccaac ttgctgacca agtcctagtt
     4381 gcaggtaagt ggtgactagc aagatcagat agaccagggg catggcgact gtcatcaaac
     4441 gattgaaact gcgtaatagg aataaaagaa ttggacgact agttagtctg gagctgatat
     4501 ttcgatacca ttcttgataa tttttcattt atgcttctca tacttttcta gaacttgttg
     4561 agatgttttc cccttggcaa gccaatccac tagcttgtcc aagcgacgta tttcctgcat
     4621 cagaggatca tcaatttcct caatacggac accgcaaatc ttccctgtaa ttgcaattcg
     4681 ttcagggaca taatatggtg cttgtctaaa aaaatctccg taagtaacat ccctatccag
     4741 acaagcaaga acatcactca cttcataccc tgtcaaccaa ctggtcactt ggtgaactga
     4801 ctctgccttt ccaccctttc gctcaacttt tgcaatcaag gctaagtaga cagcagcaaa
     4861 tttcatattg tataatttct gactcatact tctctctttc tatgtgtact agtataaata
     4921 agaaaaagaa ggccgtcaag ccttcttttg atttattctt ctgcttcatc ttctgtaaat
     4981 tgactattgt acaagtcagc gtagaagcca ccttgcgcca tcagttcctc atagttgcct
     5041 tgctcgatga tatttccatc tttcatgacc aagatcaagt ctgcatttcg gatggttgac
     5101 aagcggtggg caatgacaaa ggatgtgcgt ccttccatca aacggtccat ggctttttgg
     5161 atcaattcct ctgtccgtgt gtcaacagaa gaagtcgcct catccaaaat caaaagcggt
     5221 gcatccttaa gaagggcacg agcaatagtc aatagttgtt tttgtcctac agacaaggtc
     5281 acggtgtcat ccaagatggt atcatagcca tctggcaagg tcataataaa gtggtgaatt
     5341 cccacagcct tactagcttc catcattcgt tcatcactaa tccctatttg attatagatg
     5401 agattgtctc gaatagttcc ttcaaagagc caggtatcct gcaagaccat tgaaaaggca
     5461 tcatgcactt ctgaacgcgt catagccttg gtatccacac catcaatgtg aatacttccc
     5521 ttatcaatct catagaattt catcaaaaga ttgacaatgg ttgtcttacc agtcccagtc
     5581 ggcccaacaa tggcaacctt ttgaccagca tgagctgtcg cagagaagtc atagtcttga
     5641 acattgacac cgtccaccag aatttctcct gctgacacgt cgtagaaacg tggaatcaga
     5701 ttgaccagag ttgatttacc agaacctgtt gacccaataa aggccactgt ttgaccagtt
     5761 tctgctttaa agctaacatg ttcaataact gcctccgaat ttgccgcata gcggaaggtc
     5821 acatccttaa actcgacctg acctttgaag ttttcatcag tcagctgtac ttgaacaggg
     5881 ttttggatag aagaatgcaa atctaaaact tgattaatcc gcttagcaga gaccatagtt
     5941 cggggaagaa cgatgaagag tgctcccatg agaaggaagc ccatgacaac ctacatggca
     6001 taagacatga aaataatcat gtcactaaag agaggcagac gcgctatcgg agcagcgtcg
     6061 ttaatcacat aggccccaat ccagtaaatc gctacactca aaccacttga aatccccatc
     6121 atgataggat tcaaaatagc cataagacgg ttgacaaaca aattcaaacg ggtcaattca
     6181 tcatttactg ctgcaaattt ttcattttga taatcctctg cattgtaggc acgaacgaca
     6241 cgaatacctg ttaaactctc acgagtgata ctgttcagtt tatctgtcaa cccctgaatc
     6301 aaggactgtt ttggaaaggc tagcgtcatc aaaacggtcg tcatcaggac gttgacaatc
     6361 actaccacaa gtacggccca gagccagtat tctgaatgac ctaaaatctt cccaatagcc
     6421 cagatagcca taattgaacc acgcgttacc acttgcaagc ccatagtaat caacatttga
     6481 acttgagtaa tgtcattggt agtacgcgtc aagaggctag gaattgaaaa tttcttaatc
     6541 tctgtctgcg agtaatccaa aactcggtta aaaatatcac ttctcagcct actagtataa
     6601 gaagccgcca ctcgggatgc aaaaaatcca actgcaacta cggacaagaa ggcaagaaag
     6661 gacattccca tcatcatgct tgccggctgc cacaactcat ctaaattagt ttcttgacta
     6721 cctagcaaat ccgtaatttt cgagatatag gtcggcactt ccaactctag atagaccgaa
     6781 aagcaagtaa agagaatggc tagtaaaatc atccccattc ttttctacta attcttttgg
     6841 ctaatttctt tattctctcc tcctattccc ttgatatttg cctgtagttg accgagaact
     6901 ttctcaaaaa tcagtaattc atcttcatca atgtcttcca tcaactgctt gtctatgcgt
     6961 tcaaaaaaag ccttaacctg ttgcatctga gaacgtgctt tgtccgtcag acgaacaaac
     7021 ttagcccgct tatcaacagg actcgcctcc aattccacca aaccattttg cactatacgc
     7081 ttaaccagat tactagcaac agacttggta atattgagtt cctgctcgat atctttaatc
     7141 aagaccaagt cttggttttt ctcgcgatta tccaaaaaac gcacaacctg accttgcggc
     7201 ccacccataa attcaatgcc gcaacgtttg gcttcctttt gcaccatcag gtgaatttga
     7261 tgaccaaaac gcttaaagac taacatcggt ttacccataa tctccccctt ctaaataaaa
     7321 atagttctct ggagaataat taaatttcta tgagaactat tttcttgatt aaaaaaatcc
     7381 caagtgattt tctcacttag gatcatgttc tataggttaa attaaaaccc atctacgttc
     7441 gtataaatct tttggacgtc ttcgtcgtct tcaagaacgc tgtaaagttt ttcaaaggtt
     7501 tcaaggtctt cgcctgacaa ttccacttct gactgaggaa tcatttccaa ttcagtcact
     7561 tggaattctt caataccaga ctcacggagg gcaacgatag ccttgtgaag gtcagttggc
     7621 gctgtgtaaa ctgtgattgt accttcttgt gcttctacgt catccacatc cacatccgct
     7681 tcgagcaatt gctcaaagac tgcgtccgca tcttcacctc caaatacaat aacacctttg
     7741 ttgtcaaaga ggtaagaaac agaacctgaa gcgcccatgt ttccgccgtt tttaccaaag
     7801 gctgcacgga cattggctgc tgtacggttg acgttagaag tcaaagtatc cacaattagc
     7861 atagagccat ttggcccaaa accttcgtaa cgtccttctg taaaggtttc gtctgtgttt
     7921 cctttggctt tatcaatcgc tttatcgata atgtgttttg gcacttgggc ttgtttagca
     7981 cggtcgataa cgaatttcaa agctgagttt gattctggat ctggatcacc ttttttagct
     8041 gctacataga tttctacacc aaattttgca tatactttag agttagctcc atctttagcc
     8101 gttttcttgg ctacgatatt ggcccattta cgtcccatta ggaatctcct tttttcacat
     8161 tttaatcttt cttattataa cacaagtttt tttgattttc actagaggaa atggatttta
     8221 ttagcaaatc aagctaggat agcactttac ctgctaagac ggtcttgcct ttctatcttt
     8281 atcaacagac actcatccac attcaaaaaa caaactagac cattatctgc aaatagaaag
     8341 tttcagccaa gtttgacaaa gtcagctcaa attactgttt gaagtttgta gatataagcg
     8401 acaaaaacaa tcatactgca ccttttgttg acagtctact ccagacatat catagttcaa
     8461 gtaaatactt tgaaatccaa cagttcttat aggcgctatt gtattctaag aaatcaatag
     8521 aagagtttct aagcaaacct ctaatactca ataaaaatca aagaacaaac cagaaagcta
     8581 gccgcaggtt gctcaaaaca ctgttttgag gttgcggata gggctgacct ggtttgaaga
     8641 gattttcgaa gagtataatt tacgtgttcc caagatggag aagttagact agtacactgg
     8701 cacttctaaa ccattgctag caattgattt gttcatattt aatttcattt tttccataaa
     8761 tgggtattag atataaacag caaaatattt ccgatacgtg tcattcttga atttccaatc
     8821 atctaaaaca agtaaaggat aatcaatccc ctgtatatca aggaattggc tacccttttt
     8881 acttttttac acatcctgtt tgatagactc attttaacat cacgagcata ctccaatgga
     8941 aatcgctagg caagagataa actttcagat atccgcagag agatcatcgc ctctttttgt
     9001 cgcaagcatt ctcttctcct agtcattttc taccttatct tctacctgag gatagagagt
     9061 tgttccccaa atagaaatcg tccgcttacg cactagtggc aaatcggttt tttcataaac
     9121 cgtacgccac cattcccagg caagcccggt acactctcta attttgacag agagattacg
     9181 aacattccct tttaaaggaa tactagtggt aaagtgagcc gtcaaatcct gcccatttct
     9241 gtcccaagcc ttaggagtca agacttcctt accttgatga tcataggata attcatccca
     9301 agtaatataa tattgggcaa cataggcacc actatgatcc agcagtaaat ctccgtttct
     9361 gtaagctgta accttagtct caacatagtc tgtactgttt tgaaaggtcg caactacatt
     9421 gtcacgtaaa aaagaagttg tataggaaat cggcaagcct ggatgatctg ctgtaaagcg
     9481 actgccttct tgaatcaagt cctctaccat atccaccttg cctgttacaa ctcgggcacc
     9541 cgaacttggg tcgcccccta aaataaccgc cttcacttct gtattgtcca aaatctgctt
     9601 ccactctgtc tgaggagcta ccttgactcc tttcatcaaa gcttcaaaag cagcctctac
     9661 ttcatcactc ttactcgtgg tttccaactt gagatagact tggcgcccat aagcaacact
     9721 cgaaatatag accaaaggac gctctgcaga aattcctctc tgttttaaat cctctaccgt
     9781 tacagtatct tgaaacacat ctcctggatt tttaacagcg tctacgctga ctgtataata
     9841 aatctgctta aaattaacaa tctgaatctg cttttcacct gaatggacag agttaaaatc
     9901 aatatcaaga gaattccctg tcttttcaaa gtcagaacca aacttgacct tgagttgttc
     9961 catgctgtga gccgttattt tttcatactg cattctagct gggacattat tgacctgacc
    10021 ataatcttga tgccacttag ccaacaaatc gtttaccgct ccgcgaacac ttgaattgct
    10081 ggggtcttcc acttggagaa agctatcgct acttgccaaa ccaggcaaat caatactata
    10141 agtcatcgga gcacgatcaa ccgcaagaag agtgggatta ttctctaaca aggtctcatc
    10201 cactacgaga agtgctccag gatagaggcg actgtcgttg gtagctgtta cagaaatatc
    10261 acttgtattt gtcgacaagc tccgcttctt tctttcgata acaacaaact catcgggtag
    10321 ctgattaccc tctttgatga aacgattttc aatactttct ccctgatggg tcaagagttt
    10381 ctttttatcg taattcatag ctagtataaa gtcatttact gctttatttg ccatcttcta
    10441 cctcctaata agttcctgga ttgagttgca taaactcaga cttgttcagc gaaatcagcc
    10501 gtggttggac taagtgatcc aaaatttcct cgtacaattc ttctgagaca ttgcgtcgcc
    10561 gtctggctag ataagaagtc ggaataaccg tattatccaa cataaatacc ttatctaagt
    10621 caatcaaggt tggtcttgta aaaggattac gagctagatc cggctcttct atcataaagt
    10681 tcttgaccaa acgtctggtc aagagagctg gtttgaaggt ctgattttta accaactctt
    10741 tgtttttagt catgctgttg tcaatacaga tatacatatg attcttcaca gccaaatcgc
    10801 tactaatagt cggaaaaggc aaataaagag ctacaacatc tcctctctta atcaagcaag
    10861 agcaccccct tttctcctaa tgtaacatag acaggattga ccaagtcttc tgattgactc
    10921 agaatttcca aagtttgagt ttggcgcgct gtcaatttag tagcatcttg tctcttcaat
    10981 acaaaatgct tgtcgccaat aaccttgaca atataatcct tctccaaagc tgactggtaa
    11041 atccacatca gatgttgtct gtcctgagaa ctcaagagag aaggattttc aagcctcccg
    11101 atagtctgat aaaaatcaaa aacaggagct aactcctgcc aatctgattg gctagttgtc
    11161 aaggctagaa aaagggcttt gcgagctgat acttcttggt tagccttgag agttactttc
    11221 ccctccaagt tttttagaaa tcgggaaact ccagaaagca aatttttctc taactgcgag
    11281 aaataaaaac ctttcgttcc cagacataag tctttcatgt cgctttctct agcaaataag
    11341 agctcaaaca tttgatagta aaagaaaaat atctggcact gggtcgcgct catcttttcc
    11401 ttatcggctt ctttttttaa ccagagcaag ggcgacaggt aactggattg agacatttcc
    11461 tctacctcct actctttttt aactggagca tctgcactag ctgccacttc ttttgactgg
    11521 atactttccc actggttaat ctcctctgag ataagacctt cgcatgtctt gacaaatagg
    11581 gcaaaagcct tggtctttcc tgcatatttc tccgtttggc attgatagag gaatttttct
    11641 ttctccagga gttgcgcagt tttttggtaa gaaatccaat tttcctttgc attatacaaa
    11701 ttgataatcc cctcacacag caagccgaga ctggataagg caaccgaaat caaacggtag
    11761 cgatcacctg gcataggaat agcacaaaag acagctatga ggaaacctgc cacgatttct
    11821 gttattttta ataccttata gcgcctacga tgttgaacgc ttttctttaa aaaatgagct
    11881 atctgtacgt ctaatcgctc tgtcaggtac atttcttctg gcgtcatatt cgtaactcct
    11941 ttcatttact ttgataacca tagtataaca tatttgaata tcaaagtata gtaaaaactg
    12001 gactataact tattagactt cctgcgaaac aaaatatggt atagtagttc tatgaattat
    12061 gaagcaagta aacaactaac tgatgcacga tttaagcgtc ttgttagtgt tcagcgtacc
    12121 acttttaaag agatgttagc tgtattaaaa acagcttatc aaaaaagtcg gacaagctgg
    12181 taaaatcttg gctgacagtg gttatcaagg gctcatgaag atatatcctc aagcacaaat
    12241 tccacgtaaa tccagcaaac tcaagccgct aacagttgaa gataaagcct gtaaccatgc
    12301 gctatctaag gagagaagca aggttgagaa catctttgtc aaagtaaaaa cgtttaaaat
    12361 gttttcaaca acctatcgaa atcatcgtaa acgcttcgga ttacga
//
LOCUS       DAVLYV010000053        12299 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLYV010000053 DAVLYV010000000
VERSION     DAVLYV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12299
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.053"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            37..846
                     /locus_tag="VYO70_001467"
     CDS             37..846
                     /locus_tag="VYO70_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1713680.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNDYSSKALC
                     EYLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIHEKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKGSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWSSLLLNENCLVIGASISGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTDHLSYGNRISPQFPILLITDCLFSNYLESPERQYYYNQTIIHKEE"
     gene            851..1552
                     /locus_tag="VYO70_001468"
     CDS             851..1552
                     /locus_tag="VYO70_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1713681.1"
                     /translation="MSQIWTKEKFISQVQGGVIVSCQALPGEALYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVVSEFIKKIKEKYPNQLLMADVSNLDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            1577..2494
                     /locus_tag="VYO70_001469"
     CDS             1577..2494
                     /locus_tag="VYO70_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HEV1713682.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQDRVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHIACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAASNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            2522..4063
                     /locus_tag="VYO70_001470"
     CDS             2522..4063
                     /locus_tag="VYO70_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEV1713683.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGSSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGVVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLIANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNADSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDNRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDITEIKADSS
                     WEVRH"
     gene            4082..4540
                     /locus_tag="VYO70_001471"
     CDS             4082..4540
                     /locus_tag="VYO70_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1713684.1"
                     /translation="MIISEQSDFKRYASVNKYFSKVCDFLENTNLTDLVDGKIDIDGE
                     NVFANCMTYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDETVKKLVIKVLNEEK"
     gene            4542..6764
                     /locus_tag="VYO70_001472"
     CDS             4542..6764
                     /locus_tag="VYO70_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HEV1713685.1"
                     /translation="MKKNIKQYVTLGTVVVLSAFVANSVAAQETETSEVSTPKLVQPV
                     APTTPISEVQPTSDNSSEVTVQPRTVETTVKDPSSTAEETPVLEKNNVTLTGGGENVT
                     KELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNNLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPREVGGRDLQISGGATYIDSVIVEKKNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLRKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFENNKKTEYRNGTKVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSKDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            6804..7913
                     /locus_tag="VYO70_001473"
     CDS             6804..7913
                     /locus_tag="VYO70_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1713686.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEFEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            8070..8960
                     /locus_tag="VYO70_001474"
     CDS             8070..8960
                     /locus_tag="VYO70_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1713687.1"
                     /translation="MKIIGIDIGGTTIKADLYDEFGTSLNHFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYTLNHSIDGVGISTAGVVNANTGEIIYAGYTIPGYIGVNFTAEIEKRF
                     GLYTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRSGDKVAEETFEIFVE
                     NLTKGLLTISYLLNPEILILGGGILDSKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            8985..9308
                     /locus_tag="VYO70_001475"
     CDS             8985..9308
                     /locus_tag="VYO70_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713688.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENDANIQYISSSRRGEFVEKIIGRVV
                     EKYGH"
     gene            9298..11289
                     /locus_tag="VYO70_001476"
     CDS             9298..11289
                     /locus_tag="VYO70_001476"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEV1713689.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQIFHTSNSNHGVIEGNDALTYLMNQQQHLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNHQINRVHELERHIQSNNEEIERLIKW
                     EKLEIVPANLEQFSFCKGKVGTIPRTEDNRLYNSLLENNIEVQEIFSNDREYGVVVFY
                     QSSYSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKKYLID
                     LQWQIDYILSIYARQISKNNFLCTPHLVALEGWIEETRILYFIKVMDEHFGHSIYIYE
                     SETLTDNQDEIPIKLTNHSLIEPFELLTEMYALPKYYEKDPTPVLAPFYFTFFGMMVA
                     DLGYGLLLFLGTMLALKIFHLPSATKRFLKFFNILGVAVAIWGGIYGSFFGYELPFHL
                     ISTTSDVMTILVVSVVFGFITVFAGLLASGLQKVRMKKYAEAYNSGFAWCVILLGLLF
                     IAVGMLMPDMRPLFVLGKWVSIFNAVGILIVSIIQTKSLSGIGAGLFNLYNISSYIGD
                     LVSFTRLMALGLSGASIASAFNLIVGLFPGILAKLTIGLVLFILLHAINIFLSLLSGY
                     VHGARLIFVEFFGKFYEGGGKPFQPLKASEKYIKVITKN"
     gene            11305..11781
                     /locus_tag="VYO70_001477"
     CDS             11305..11781
                     /locus_tag="VYO70_001477"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEV1713690.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            11815..>12299
                     /locus_tag="VYO70_001478"
     CDS             11815..>12299
                     /locus_tag="VYO70_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713691.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSLIIKK
                     KEAEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALLEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNYLFSEQPISNESGLLI
                     S"
BASE COUNT         4165 a         1772 c         2432 g         3930 t
ORIGIN      
        1 aagaatgtaa acgtttactt caactaagga gctcatatgt tactgcaaaa agaactaatt
       61 ccaatgatag aagctaactt accaaatatg gcatatgctg aaaaagacat tgctaaattc
      121 ttcttaaaac agcaacctct gaatgattat tcatctaaag cattgtgcga ataccttaat
      181 gtatccaaag caacattgac tcgatttgcg aaaaaatgtg gttttaaagg ttttagacaa
      241 ttcattttca aataccaaga gatgattcat gagaaagaaa agttggcatt atatacagag
      301 gcaacagaaa aagttttatc cgactatgag gaaatgttga gaaaaactta cacggttctt
      361 gatgaagttc aacttgagcg tattgctgag atgatagaaa ctgctgagcg tgtatatctc
      421 tacggtaaag gaagttctgt tcttgcttta caagaaatga agatgagatt tatgcgtctc
      481 ggagtgattg gtgaagtatt atcagacgag gatatgattt tgtggagtag cttactactt
      541 aatgaaaatt gccttgtcat tggagcatcc atttcaggtc aaactgatat tgtactagaa
      601 ggtctacaaa aagctgcaga taaaggcgct aaaacagttt taatgactac aagaaaattt
      661 gacgaagaag attgtttctt tgatgaacta ttgttattag cttcgaccga tcatctctcg
      721 tatggcaatc gcatatcacc tcagtttcca atacttttaa ttacagactg cttattctct
      781 aattatctgg aaagtccaga gagacaatat tattacaatc aaactattat ccataaggag
      841 gaataataaa atgagccaaa tttggactaa agaaaaattt ataagccaag ttcaaggtgg
      901 agtcattgtt tcttgtcaag ctttacctgg tgaagccctt tataatgaag aatttagctt
      961 gatgcctttt atggctaaag cagctttaga ggcaggagca gtgggcattc gcgcaaattc
     1021 tgtgcgtgat attaaagcaa ttcagaaagt agtagattta ccaataattg gaattatcaa
     1081 aagggattat ccacctcaag aaccatatat tactgctacg atgaaagaag tagatgaact
     1141 tgtagaatgc ggaacaacag tcattgcatt tgatgcaact ttaagaccaa gatatgatgg
     1201 cttagttgtc agtgaattta tcaaaaaaat aaaagaaaaa tatccgaatc aattgctgat
     1261 ggcggatgta agtaatttag atgaaggtct ctatgcattt aaatcaggcg ttgattttgt
     1321 tggtacaaca ttatcaggtt acacaagtac aagtgtacaa tcagatgagc ctgattttga
     1381 actaatgaaa aaattggctg attttaatat tccggtaatt gccgaaggaa aaattcatta
     1441 tccagaacaa ttaaaaaaag cttatagttt aggtgttacc agtgtagtca ttggtggagc
     1501 gattacacgt ccaaaagaaa ttgctcagcg atttattaat gtcatcaaat aaaaaacaat
     1561 ataaaggaga aatctgatga aagatttaac taaatacaaa ggcgttatcc ctgcatttta
     1621 tgcttgctat gatgaaaatg gtgaaattag ccaagatcgt gtaaaatctc tggtacaata
     1681 tttcattgac aaaggtgtaa aaggtatcta tgtaaatggt tcttcaggtg aatgtattta
     1741 ccaaagtgta gaagatcgta aacaaattat tgaagctgtt atggaagttg ctaaaggtaa
     1801 attaacagtt atcaaccata ttgcatgtaa taacacgaaa gatagtatcg aattggcaaa
     1861 acattcagaa agtgttggag tcgatgctat tgcagctatc ccacctattt atttcaaatt
     1921 gccagagtat tcaatcgcag catattggaa tgcaatgagt gaagctgcgt caaatacaga
     1981 ttttattatc tataatattc cacaattggc aggggttgcg ttgactggta gtttgtatgc
     2041 aacaatgcgt caaaatcctc gtgtgattgg agttaaaaat tcttctatgc ctgtacaaga
     2101 tattcaaatg tttgtagctg caggtggaga agattacatt gtattcaatg gtccagatga
     2161 acaatatctt ggtggtcgct tgatgggagc agaagctggt attggtggta cttatggcgt
     2221 tatgccagat ttgttcttga aattggaaag tttgattcaa gaacgagatt tagatacagc
     2281 taaaaaactt caatatgcta tcaatgaagt tatctataag atgatatcag gtaaggcaaa
     2341 tatgtatgct gtagcaaaag aagttttgcg tctaaatgaa aaacttgatt taggttctgt
     2401 tcgtcaacct ttagaagcat tggcagaagg tgacttggaa gttgcaaaac aagcagcaga
     2461 acttattcaa caagcacgaa aagaattttt ataataaata aaatcaatat ggagatatgt
     2521 tatgggcaca acaggattta caataattga cttaattatc ttgattgttt atttacttgc
     2581 ggtgttggtt gcaggtatct atttctctaa aaaagagatg aaaggaaaag agttctttaa
     2641 aggagatggt tcggttcctt ggtatgttac ttcggtatcc atttttgcca caatgctcag
     2701 tccgatttcc ttcttgggac tcgctggtag ctcttatgca ggtagctgga ttttatggtt
     2761 tgctcaatta gggatggtag tagctattcc actgacaatt cgttttatct tacctatctt
     2821 tgcacggata gacatcgata cggcatatga ttacttggat aaacgtttta attctaaagc
     2881 acttcgtatt atttcagcac tcttgtttat tatttatcaa ttgggacgta tgtctatcat
     2941 tatgtacctc ccatcagctg gtttatcagt attgacagga attgacatca atattttgat
     3001 tattttgatg ggtgtagttg caattgttta ttcttatact ggtggtctaa aatccgtatt
     3061 atggacagac tttattcaag gtgtgattct gattagtggt gtcgttttag ctttatttgt
     3121 actgattgct aatattaaag gtggctttgg tgcagtagca gaaacattag caaacgggaa
     3181 attccttgct gcaaatgaaa aacttttcga tcctaacttg ctttcaaact ccatcttttt
     3241 aattgtgatg ggttcaggct ttacaatctt gtcttcctat gcttcatctc aagatttggt
     3301 tcaacgtttt actacaacac aaaatattaa gaaacttaat aagatgttgt tcacaaacgg
     3361 tgttttgtca cttgcaactg caacagtctt ttacttgatt ggtacaggct tgtacgtatt
     3421 ctatcaagta caaaatgcag atagtgcagc tagcaatatc cctcaagacc aaatctttat
     3481 gtactttatt gcataccagt taccagtagg tatcacaggt ttgatcttgg cagcgattta
     3541 tgcagcatct caatcaacta tttcaacagg tttgaactct gttgcaactt catggacatt
     3601 ggatattcaa gatgtcattt ctaaaaatat gtcagacaat cgtcgtacga aaattgcaca
     3661 attcgtatct ctagcagtag gtttattctc aattggtgtt tccattgtca tggctcactc
     3721 agatattaaa tctgcatacg aatggttcaa tagtttcatg ggacttgtac ttggtctact
     3781 tggtggtgta tttattcttg gatttgtttc taaaaaagca aataaacaag gtgcttatgc
     3841 agcgctgatt gtatcaacca tcgtcatggt atttattaaa tacttccttc ctccaacagc
     3901 tgttagctac tgggcatatt cattgatttc aatctctgta tcagtagttt caggttatat
     3961 tgtatctgtt cttactggaa ataaagtatc tgcacctaaa tatacaacga ttcatgatat
     4021 tacagaaatt aaagcggatt caagttggga agttcgtcac taatagaaaa gagaaaataa
     4081 tatgattatt tctgaacaat ctgattttaa acgatatgct tctgtcaata aatatttttc
     4141 aaaagtctgt gattttttag aaaatacaaa tttgacagat ttagttgatg gaaaaattga
     4201 tattgacggg gaaaatgttt ttgcaaattg tatgacttat ctagctgatg gagttccagg
     4261 agatattttt gaaactcata agaaatattt agatattcat attgttgttg aaaatactga
     4321 aaaaatggca gtaacttccc cagttcgtgc ccagtcacgt gtacctttta gcgaagagaa
     4381 ggatattgct ttttacgata gcaaagacta tcaaattgtt gaattgcttc cagggaatat
     4441 gttggtgact tttgaagaag atcttcacca accaaagatt cattgcaatg atgaaactgt
     4501 taaaaaactt gttatcaaag ttttaaacga ggaaaaataa aatgaaaaaa aatattaaac
     4561 aatatgtaac cttaggtact gtagtggtat tatcagcatt tgttgctaac tcagttgcag
     4621 ctcaggagac tgaaacttct gaagtatcaa caccaaagtt ggtgcaacct gttgcaccaa
     4681 cgactccgat ttcggaagta caacctacat cggataactc ttcggaagtt actgtacaac
     4741 ctcgaacagt tgaaactact gttaaggatc catcttctac agcggaagaa actcctgtct
     4801 tagaaaaaaa taatgttact ttaacagggg gcggagaaaa tgttactaaa gagttaaagg
     4861 ataaatttac tagcggtgac tttactgtag tgattaagta caatcagtca agtgagaaag
     4921 gcttacaagc tctgtttgga atatctaatt ccaaacccgg tcaacaaaat agttatgtag
     4981 atgtgttcct tagagacaat ggtgagttgg ggatggaagc gcgtgatact tcttccaata
     5041 aaaataacct agtatccaga cctgcttcag tttggggtaa gtacaaacaa gaggctgtga
     5101 ctaacactgt tgcagtagta gcagattcag tcaaaaaaac atattcttta tacgcaaatg
     5161 gtacaaaagt agtagaaaag aaagtggata atttcctaaa catcaaggat attaaaggta
     5221 ttgattacta tatgcttggg ggagtgaaac gtgcaggaaa aacggcgttt ggttttaacg
     5281 gaacactaga aaatatcaaa ttctttaata gtgcattgga tgaagaaact gttaaaaaga
     5341 tgacaacaaa cgctgttact ggacatttaa tttatacggc taatgataca acaggttcta
     5401 actatttccg tattccagtt ctgtatactt ttagcaatgg tcgggtattt tcaagcattg
     5461 acgctcgtta cggtggaact catgatttct tgaataaaat taatattgct acaagttata
     5521 gtgatgataa tggtaagaca tggactaaac caaaattaac attggcattc gatgattttg
     5581 cgccagtacc attagaatgg cctcgtgaag ttggtggacg tgacttacaa atcagcggtg
     5641 gtgcaaccta tattgactct gttattgttg aaaaaaagaa caaacaagta ctcatgtttg
     5701 ctgatgtgat gcctgctgga gtaagtttta gagaagcaac tagaaaagat tcaggttata
     5761 aacaaattga tggtaattat taccttaaat taaggaaaca aggtgatact gattacaatt
     5821 atactattcg tgagaatggt actgtatacg acgatcgtac caacagacca actgaatttt
     5881 cagtagataa aaatttcggt attaaacaaa atggtaatta tttgacggta gagcaatatt
     5941 cggtttcatt tgaaaataat aaaaagactg aatatcgtaa tgggactaaa gtccatatga
     6001 atatcttcta caaagatgca ttgtttaaag tagtgccaac gaactatatt gcatatattt
     6061 ctagcaatga tcatggcgaa tcttggtctg caccaacttt attacctcct ataatgggac
     6121 ttaatcggaa tgcgccatat ttaggtcctg gacgtggaat cattgaaagc tcaactggac
     6181 gtattcttat tccgtcttac actggtaaag agtctgcgtt catttatagt gacgataatg
     6241 gagcatcttg gaaagttaaa gtagtgccac ttccttctag ttggtcagca gaagcacaat
     6301 ttgtagaatt gagtccagga gtaattcaag catatatgcg tacaaataat ggtaaaattg
     6361 catatttaac aagtaaagac gcaggtacta cttggagtgc accggaatat ttgaaatttg
     6421 tttcaaatcc aagttatgga acacaattat caatcatcaa ttatagccaa ttgattgatg
     6481 gtaaaaaggc tgtcatttta agtactccaa actccacaaa tggtcgtaaa cacggacaaa
     6541 tttggattgg tctaattaat gatgataata caattgattg gcgttatcat cacgacgttg
     6601 attatagtaa ctatggatac tcatattcaa cattgacaga gttaccaaat catgaaattg
     6661 gattgatgtt tgaaaaattt gattcatggt ctcgtaatga acttcatatg aaaaatgttg
     6721 taccatatat aacatttaag attgaagatc tgaaaaagaa ttaaagctga aatttgaaaa
     6781 tatataaaaa gaggataaaa attatggtaa attacggtat tgttggagct ggatattttg
     6841 gagctgattt agctcgctca atgaacaaaa ttgaagatgc aaaagtggtt gcggtatttg
     6901 acccaaatca tggagaagaa gttgctcaag agttgggatc agatgtttgt gcaagtttag
     6961 atgaacttgt agcacgtgaa gatattgatt gtgtgatcgt agcttcacct agctaccttc
     7021 accgtgaacc agttgtgaaa gctgctcaac atggcaaaca cgtattttgt gaaaagccaa
     7081 ttgcattgtc ttatgaagat tgtaaagcca tggttgacgc atgtaaagaa aataatgtca
     7141 tctttatggc tggtcacatc atgaacttct ttaacggtgt acaccatgct aaagaattga
     7201 ttactcaagg taaaatcggt aaagttcttt attgccatgc tgctcgtaca ggttgggaag
     7261 aacaacaacc aactgtatca tggaagaaac ttcgttctca atctggagga catttgtacc
     7321 accatattca tgaattagat tgcattcagt ttatcatggg aggacttcct gaaaaagcga
     7381 caatggtagg aggcaatgta tatcataaag gtgaaaactt tggtgatgaa gatgatatgc
     7441 tcattgtaaa cttagaatac tctgatgatc gttatgctgt tttggaatat ggtaatgctt
     7501 tccgttgggg tgaacactac gtcttgattc aaggaactga aggagctatc aaacttgact
     7561 tgttcaatac tggcggtact cttcgtgtta aaggtgaagg agaatcacac ttcttagttc
     7621 atgaaactca agaggaagat gatgatcgta cagctatcta taccggtcgt ggtatggatg
     7681 gagcaattgc gtacggtaaa ccaggagtac gttgcccatt atggttgcaa acatgtattg
     7741 ataaagaaat ggaatatcta catgacatca ttaaaggtgg agaaattaca gaagaatttg
     7801 aaaaacttct caatggtgta gctgctttag aatcaatcgc taccgctgat gcatgtactt
     7861 tatcagttaa agaagatcga aaagtaagtc tttcagaaat cacaaatgct taacttttgt
     7921 aaaacagaat agtaaattct tgtcattata taatttctaa agttctgtga tacaactcat
     7981 tgaataaaga aatagagatg ggactgggat aatgcccagt cccatttttt atcaaaaagt
     8041 aatgagatca aaaatgtggg agtgttgaaa tgaagattat aggtatcgat attggcggaa
     8101 caacaattaa ggcagattta tacgatgagt ttggaacgag tttgaatcat ttcaaagaga
     8161 tagaaacaat tattgactat gatttgggaa cgaatcagat attaaatcag gtctgtgatt
     8221 taattggtga gtatacttta aatcattcaa ttgatggtgt tgggatttcc actgctggag
     8281 ttgttaatgc taatactgga gaaatcatct atgcaggcta tacaatacca gggtatatcg
     8341 gagtaaactt tactgccgaa atagaaaaac gttttgggtt gtatactttt gttgaaaatg
     8401 atgttaattg tgctgcatta ggtgaattgt ggaagggaca agccaaagat aagaaaaatg
     8461 tagtaatggt tactattgga acaggtatag gaggcagtat tattgtcaac ggacaaattg
     8521 ttaacggatt taactatact gctggtgaag taggttatat tcctgtaggt aattcggatt
     8581 ggcaaagtaa agcctcaaca accgcattga ttcatttata tcaaaaaaag agcttgaaaa
     8641 ctaatcaaac tggacgtact ttcttcactg atttaagatc tggagataaa gttgctgaag
     8701 aaacttttga aatttttgta gaaaatctaa caaaaggttt attaacgatt tcttatctac
     8761 ttaatccaga aattctcata ttaggaggtg ggattctgga tagtaaggat attttgttac
     8821 ctgaaattca aagttcttta gctaaaaatg caatggataa taggttttta cctaaaaatc
     8881 ttgtggcagc tacattagga aatgaagctg gtcgtatagg agctgtaaaa aatttcttag
     8941 atagaatttc taataaatag tatgtaagat aaggaggtgt cacaatgact aactctgtat
     9001 tttcgacaat gcaagatatt gagaatgttg caaccgatat tataaaatca tatgataatg
     9061 agatttatac ttataaagct gtttcccaag aagaattgga aaaactagaa aaaagttatg
     9121 atgaaaaaag tcacgaagaa ttagtttcaa tagaaagcaa tttagaaatg aaacaacaga
     9181 accttattga tgaggttaat aaaacaatca aggaaaatga tgcaaatatt cagtatattt
     9241 catcaagtag gagaggagaa tttgtagaaa aaattattgg tagggtggta gaaaaatatg
     9301 gccattagtc agatgaaaag aatctctcta ctattttcta aaagtagtct tgatgatgtt
     9361 ttaaaaacta ttcaagaact agagtcagtg cagttccgtg atttaaaggt tcaggataac
     9421 tggtcagaag ctctagaaaa agatgaagtt gtatttccaa ctattcaaat ttttcatact
     9481 tctaattcca atcatggggt tattgaggga aatgatgcct tgacttattt gatgaatcaa
     9541 caacaacatt tagaagcaac tgtagagaaa ttacaagaat acctaccgaa agaaaacacg
     9601 tttaaattat tgcagcaacc tccgataact acctcttatg aagaattaga gaaatttggt
     9661 aaagctaatg ttgctgaggg tgttcttaaa aaagtgaatc atcaaattaa cagagttcat
     9721 gaattagaaa gacacattca aagtaataat gaggaaatag agcgattaat aaagtgggaa
     9781 aaattagaaa ttgttcctgc gaatttagaa caattttctt tctgtaaagg aaaagtcgga
     9841 acaattccaa ggactgaaga taatcgctta tacaatagtc ttttagaaaa caatattgaa
     9901 gttcaagaaa tattttctaa tgatagagag tacggtgttg ttgttttcta tcagtctagt
     9961 tactctatag attttgatga atacttattt gaaccatttg attattctag aaaggaatta
    10021 ccgaagcagc gagtagtaga tttagatcaa gaaaacatgc agttaataac tgaaaaagag
    10081 aatattatcg catcgttgca agattcaaag aaatatttga tagatttaca atggcaaata
    10141 gactatattt tatctatcta tgctcgtcaa atctctaaga ataacttttt gtgcactccg
    10201 catctagttg cattagaagg atggatagaa gaaactcgta ttttatattt tataaaagtt
    10261 atggatgagc attttggaca ttctatttat atttatgaat cggaaacatt gacggataat
    10321 caagatgaaa tacctatcaa attaacgaat cattctttaa ttgaaccatt tgaattattg
    10381 acagaaatgt atgctctgcc caaatattat gagaaagatc ctacacctgt attagcacca
    10441 ttttacttta cattttttgg aatgatggtt gctgatttag gctatggttt actattgttt
    10501 ttaggaacaa tgttagcatt aaaaattttt catctacctt cagcaactaa gagattttta
    10561 aaattcttta atatattagg ggtagccgtt gcaatttggg gtggaatcta tggctcattt
    10621 tttggatatg agttgccatt tcatctgata tctacaacct ctgatgtcat gactatatta
    10681 gtagtgtcag ttgtgtttgg gtttattaca gtatttgcag gtttgttagc ttcaggacta
    10741 caaaaagtaa gaatgaagaa atatgcagaa gcatataatt caggatttgc gtggtgtgtt
    10801 attctgcttg gcttgttatt tattgctgtt ggaatgttga tgcctgatat gagaccgtta
    10861 tttgtattag ggaaatgggt atctattttt aatgctgtgg ggattttgat tgtttctatt
    10921 attcaaacca aaagcttgtc aggtattgga gcaggattgt ttaatctata taacatttca
    10981 tcttatatag gtgatttagt tagtttcact cgattgatgg cattaggatt atctggagca
    11041 agtatagcat cagctttcaa tttaattgtt ggtttgtttc cgggaatatt ggctaaactg
    11101 acaattggat tagtattatt cattctttta catgcgatca atatttttct atcgttacta
    11161 tcaggatatg ttcatggagc acgtctgata tttgttgaat tttttggtaa gttttatgag
    11221 ggtggaggaa aaccatttca acctttgaag gcttctgaga aatatattaa ggttattaca
    11281 aagaattaat ggaggatata tataatggaa catttagcaa cttatttttc aacctatgga
    11341 ggagctttct tcgctgcatt gggaattgta ttggcggttg gattaagcgg tatggggtct
    11401 gcttatggag ttggtaaggc tgggcaatct gccgcagctt tactgaaaga acagcctgaa
    11461 aagtttgcct cagctttgat attgcaatta ttgcccggaa cacaaggatt atatggtttt
    11521 gttattggaa ttttaatttg gttgcaatta actccagaac ttcctttaga aaaaggcgtt
    11581 gcttatttct ttgtagctct tccaattgct attgtaggat acttttcagc taagcatcaa
    11641 ggaaatgtag cagtagcggg aatgcaaatc ttggctaaaa gaccaaaaga attcatgaag
    11701 ggagcaattt tagctgccat ggtagaaacc tatgcaattc ttgcttttgt cgtatcattc
    11761 attttgaccc ttcgtgtata agaaataaat ttgcaattca aaggaggtgt ctaaatgagc
    11821 aatttagaaa acttacgaga gtctgttatt gaacaagctc atgaaaaagg gcgtatgaaa
    11881 ttattggatt ccaaaaagaa gattgatgat gaatttgaaa tgcaaaagtc gctcattata
    11941 aagaaaaaag aagctgaaca tgaacgaaag ttaaaagaat tgcaacagaa atatcaaata
    12001 atttttcaac aattaaaaaa taaggaacgc caatcaacgt tagtatcaaa acagaaaata
    12061 ttaaaagaac tttttcaatc tgctttacta gaaatggaat cttggagtgc agataaagaa
    12121 atggagttca tctatcgaat tctggaacga tattcacaac aagaggtcat agtaaccttt
    12181 ggggaacgga ctttagctaa attcaatttg gaacaattag agaaattgaa attctctttt
    12241 ccaaattatt tatttagtga acaacctatc tcaaatgaat caggcttact tatttcaat
//
LOCUS       DAVLYV010000054        12029 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLYV010000054 DAVLYV010000000
VERSION     DAVLYV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12029
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.054"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            74..1063
                     /gene="manX"
                     /locus_tag="VYO70_001479"
     CDS             74..1063
                     /gene="manX"
                     /locus_tag="VYO70_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1713692.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQAAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            1091..1894
                     /locus_tag="VYO70_001480"
     CDS             1091..1894
                     /locus_tag="VYO70_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1713693.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            1918..2829
                     /locus_tag="VYO70_001481"
     CDS             1918..2829
                     /locus_tag="VYO70_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1713694.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2885..4219)
                     /gene="pepC"
                     /locus_tag="VYO70_001482"
     CDS             complement(2885..4219)
                     /gene="pepC"
                     /locus_tag="VYO70_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1713695.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(4331..5053)
                     /locus_tag="VYO70_001483"
     CDS             complement(4331..5053)
                     /locus_tag="VYO70_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1713696.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEIFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVEIFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILLASIA"
     gene            complement(5276..5506)
                     /locus_tag="VYO70_001484"
     CDS             complement(5276..5506)
                     /locus_tag="VYO70_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1713697.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            complement(5516..6757)
                     /locus_tag="VYO70_001485"
     CDS             complement(5516..6757)
                     /locus_tag="VYO70_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1713698.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            complement(6779..6931)
                     /locus_tag="VYO70_001486"
     CDS             complement(6779..6931)
                     /locus_tag="VYO70_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713699.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            complement(6952..7230)
                     /locus_tag="VYO70_001487"
     CDS             complement(6952..7230)
                     /locus_tag="VYO70_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HEV1713700.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            complement(7223..7486)
                     /locus_tag="VYO70_001488"
     CDS             complement(7223..7486)
                     /locus_tag="VYO70_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1713701.1"
                     /translation="MSKMSMSIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            complement(7588..11979)
                     /locus_tag="VYO70_001489"
     CDS             complement(7588..11979)
                     /locus_tag="VYO70_001489"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1713702.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFETKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGIEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNAAFDVGFMNANYERHGLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFINKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
BASE COUNT         3333 a         2877 c         2322 g         3497 t
ORIGIN      
        1 aaacggttta tacttaaaca gtcttaaagt tttcttaaat gaaaactaaa acaaaaaagg
       61 aggaatgaca ataatgagta tcggaatcat tattgcgagc cacggcgaat ttgctgcggg
      121 tattcatcag tcaggatcta tgatctttgg tgaacaagaa aaggttcaag ttgtgacctt
      181 tatgccaaat gaaggtcctg atgatctata cgctaagttt aataacgctg ttgctgcatt
      241 tgacgcagaa gatgaggttc tagttttggc tgacctttgg agtggttctc catttaacca
      301 agctagtcgc gtgatgggag aaaatcctga gcgtaagttt gccatcatca caggacttaa
      361 cttaccgatg ttgattcaag cctacacaga gcgcctcatg gacgctgctg caggtgtaga
      421 aaaagtcgct gctaatatca ttaaagaagc caaggatggc atcaaagctc ttccagaaga
      481 gctaaatcca gttgaagaag ttgcaagcgc tgcagctgct ccagttgccc aagctgctat
      541 cccagaagga actgttatcg gagacggtaa attgaaaatc aatcttgccc gtcttgacac
      601 acgtctactt cacggtcagg ttgcaactgc ttggactcca gattcaaaag caaatcgtat
      661 catcgttgct tcagataacg tggctaaaga cgaccttcgt aaagaattga ttaaacaagc
      721 agctccaggt aatgtcaagg ctaacgtggt tccaattcaa aaactgattg agatttcaaa
      781 agacccacgt tttggagaaa cacatgccct tatcttgttt gaaacacctc aagatgccct
      841 tcgtgccatc gaaggcggcg tgccaatcaa gactcttaac gttggttcta tggctcactc
      901 aacaggtaaa acattggtca ataccgtttt gtctatggac aaagaagatg ttgcgacatt
      961 tgaaaaaatg cgtgacttgg gtgttgaatt tgatgtccgt aaagtaccaa atgattctaa
     1021 aaaagatttg tttgacttga ttaacaaagc caatgtcaaa taagccatta tttatgaaag
     1081 gattttaaac atgtctatta tttctatggt tttagtagtc gttgtagcct tctttgcagg
     1141 tcttgaaggc atcctcgacc agttccaatt tcaccaacca cttgtagcct gtacccttat
     1201 tgggcttgta acaggtcact tggaagcagg gattatcctc ggtggatcgc ttcaaatgat
     1261 tgcccttggt tggtcaaata tcggtgctgc tatcgctcct gatgctgcac ttgcttctgt
     1321 cgctgctgcc attatcatgg ttcttggtgg tgactttacc aagactggta tcggtgttgc
     1381 ccaagcggtt gctatccctc ttgccgtagc tggacttttc ttgacaatga ttgttcgtac
     1441 aatttcagtt ggtttggttc atactgcaga tgctgccgct aaaaaaggtg acttcggcgc
     1501 tgtggagcgt gcgcatttca ttgcgctact cttccaagga cttcgtatcg cgcttcctgc
     1561 agctcttctc cttatggtac caactgaaac tgtacaaagt atccttagtg ccatgccaga
     1621 ctggctcaaa gatggtatgg ctatcggtgg tggtatggtc gttgccgttg gttacgccat
     1681 ggttatcaac atgatggcaa ctcgtgaagt atggccattc ttcgctcttg gtttcgttct
     1741 cgctgctgtg tcagatatta ctctaatcgg attcggtgct atcggtgttg ctatcgctct
     1801 tatctacctt cacctttcta aaactggtgg aaatggtggc ggaggagccg caacttctaa
     1861 cgacccaatc ggcgatatcc tagaagacta ctaagataag aaaggactga aaacatcatg
     1921 actgaaaaac ttcaattaac taaatcagat cgtaaaaaag tttggtggcg ttcaaccttc
     1981 ttacaagggt cttggaactt tgaacggatg caaaacttgg gctgggctta tacactcatt
     2041 ccagctatca aaaaactcta tactaaaaaa gaagatcaaa tcgctgctct tgagcgtcac
     2101 cttgagttct tcaacactca tccatacgta gctgctccag tcatgggggt tactcttgcg
     2161 cttgaagaag aacgtgctaa cggtgtggaa atcgatgacg ctgctatcca aggggtcaaa
     2221 atcggtatga tgggacctct tgctggtatc ggtgacccag tattctggtt tacagtacgc
     2281 ccaatccttg gatctctcgg tgcttcactt gcccttactg gcaatatctt gggtccactc
     2341 ctcttctttg ttgcatggaa cttgattcgt atgtcattct tgtggtatgt tcaagagatt
     2401 ggatacaagg ctggatcaga aatcactaaa gatatgtctg gtggtatcct tcaagatatc
     2461 actaaaggag cttctatcct tgggatgttc attcttgctg tccttgttca acgctgggta
     2521 aatattaaat ttgctttcga tgtttctaaa gttcaactag atgaaaaggc ttatatccat
     2581 tgggataaat tgccagaagg gtctaaaggt atccaagaag cattcgcaca agtaggacaa
     2641 ggattgtctc aaactcctga aaaagttact actttccaac aaaacttgga tatgttgatt
     2701 cctggattat caggactact ccttacttta ctttgcatgt acttacttaa gaaaaaagta
     2761 tctccaatca ctattatcct tgccctcttc gcagtgggta ttgtggcaca tgttcttcac
     2821 atcatgtaat caagcaacta aaaaggaacc aggttctaaa atctgattcc ttttttctat
     2881 gcttttattc agccaaggct cccattggat cccatggtgc aagtacaatt ggttctgctc
     2941 cataggcagc ttgttcttct gctgtcagca attccttacg aacaacgatt tggtatgtgt
     3001 attcgtccat ccaagcgtct gaggcaacaa agtaaccatc tgtaccgacc ttgtctcccc
     3061 atgagttttc aaccttccac ttggttgatt taccattttc gtccaagtca acacctgtca
     3121 agaccatggc gtgggtcatc aagctttcgc tatagtccaa acgtccagcc ttgtcttgag
     3181 taagtttaat gtccatgctt gattcaaagt cataaacatc tgtcgcaagg atgccagcct
     3241 tacggttact gagctggccg acatcagaac caaaccaaac agtctcacct gcttgcattt
     3301 gggcaatcgc caattctttc aagcgctcca ttggaacgtt gatgtaacga actgcacggc
     3361 taccaaccac attccccaac atctcaactg tgtaagattt tccgtaaggt ttatcagcag
     3421 ttggagcatt gataacagaa acgtagtctt ctaaaggaag attgacatat ttcttgtaaa
     3481 actcttgtgg tgtgattcct ttttcacttt tgtagttgtt atctttatcg cgataagcaa
     3541 agtcaaactt gcgtggtgga agtcctaatg acatagcaag aaagttaaag atttcttgca
     3601 agaggtcttc tttcttagct tgaacagtcg cttgatctgc accagaaaca agcaagtcac
     3661 gcaagatttg agcatcttga cgaagcaatt tattaaggat cgcatttagc tcacgactgc
     3721 tgctagatga aacagactca ggataaactg acttaggcac gacaccgtat ttttcaaaga
     3781 gggaaacgac catatcccat tgaccaccat cttgttgagg tgtttgaagt aagaagctaa
     3841 ccttgcggct agtcaattct tggtctgaag tcgcaatgac ttgctccaag aaccagtttg
     3901 atttctcata cttatcccag aagaaagtgt gggcttgtga caactcaaag ttctccaatt
     3961 tgtattgcga gatgagtttg tggcggaagg tgttgagagc cgcaaacatc cagcaacgac
     4021 cagacgcttt ctggttagtg accttgtcct tggttaaatc caatgagaaa acaggtgtgt
     4081 tgtctacatg gctttggcga cgttccagag ctgcaaaaat tccgttgtgg ctagcagcat
     4141 tttcaatcgc ttggtatttt acatttgctt catagttggc aaatagttta tcagtaaatg
     4201 attcttgaat cgcgttcata gattcctcct tttattctac agtctattat aactcttttc
     4261 acaaaggaag caactaaaaa acataaaagg taaggacatt ctccctcacc tttttcgaat
     4321 ataaatcaaa ttaagcaata ctagcaagga gaatttctaa ttcttccttg gtcaagcgac
     4381 gccattcccc tcgttccagg ttctcatcta atactaaagt tcccatagtc aatcgttgca
     4441 agtccaccac ttccttgcca cagtagccca ccatacgctt gatctgatga aacttccctt
     4501 ctgcaatggt cacacggatt tggctttgat tcttttctgt atctatggat acaagctcca
     4561 gtatagcggg ttgacaggta aagtctttga gaggaatacc ctcagcaaat atctccacat
     4621 cttcttgggt catgattccc ttgacttgtg ccagataagt cttgtccaca tgacgcttgg
     4681 gcgaaagaag aacatgagcc agctgaccat cattggtcaa gagcaaaaga ccatgcgtgt
     4741 caatatccaa gcgtcctact gggaaaattt ccttactccg cgccaagtca tccaacaagt
     4801 ccagaacggt tctgtgcttg ggatcctcag tcgctgatat aactcctttg ggcttgttca
     4861 tcatgtagta gacaaactct tcatactcca acacttgccc atcaaagcga atctcatcta
     4921 ttttttcatc aatctgcaat ttagctgatt tttctttttt accatttaca gtcacgcgcc
     4981 cagccttgag caagtttttg acctcagtcc gacttcccac cgcacaggca actaaaaatt
     5041 tatctaatct catagaacta ttatatcata tcaaaaggag gctggtacaa tgaccaacct
     5101 ccttttcgtt tcatactctt caaaaatctc ttcaaaccgc gtcaacgtcg ccttgccgta
     5161 tatatgttac tgacttcgtc agttctatct gcaacctcaa agcagtgctt tgagcaacct
     5221 gcggctagtt tcctagtttg ctctttgatt ttcattgagt atcagattta agaaattaac
     5281 ttcctcgtct ccaaaaaata gctaagacaa tcatggcacc taaaacagct gggataatag
     5341 ctgttcctga taaaactggc ccccaagttc caaagagcaa gtgacctaga aaggctccga
     5401 tccaaccgag aaacattttt ccaaaacatc ccattcgctc tccacgattg gtcatagcac
     5461 ctgctaaaaa tcccactagg agaccaacga acatacttcc taacatatta tctccttaat
     5521 ttgcccaatt cccattacgg aaaagaggta ctcgcgttcc atcctcacga ataccatcga
     5581 tatccatttg gttagaacca atcataaagt ctacgtgaac atctgaacgg ttaagccctg
     5641 cagcttcaag ctcctcttcg ctcatctccg ctccatcaac aacgctagtc gcataggctg
     5701 caccgatagc caagtggttt gacgcatttt catcgaaaag ggtgttaaag aaggtaatgc
     5761 ctgactgaga aattggactt ggatctggta ccaaggcaca ttcacccaag gcacgcgcac
     5821 ccgcattttc aaagacaagg tctttcataa cctgatcacc cttctcagca gtgatatcta
     5881 cgatttgtcc atccttaaag gtcaccttaa tgccttcaat gatatttccg ttgtagctaa
     5941 gcggttttgt agaagtgaca taaccatctg cacgacggaa gtcaggcgct gtgaagacct
     6001 cttctgtcgg catatttggc aagaattctt cgccctgtgc attgacagca ccagctgatt
     6061 cccaaacgtg gttctttggc aaaccaagtg ttaaatctgt tcctggcgct gtgtagtgaa
     6121 gggctgaaaa ttgctcctta ttaagcatat cggccttgct cttgagaatg gctgcatgtt
     6181 cctcccaagc cttaacagga tctgcttcgt agacacggca agttttgaaa atttggtccc
     6241 aaaggaaatc aactgcttct tcgtcgctcg cagcatttgg gaagactttc ttagcccact
     6301 caagtcctgc tgcagctgct acagtccagc taactttgtt agattgagtt gcgattcgca
     6361 ttggcttcat ggcaagtccc atagctttag cagaagctga aagcttgtca gcgtccactc
     6421 cgttcaaggc acctggatca gatgaacgaa ctccaagacg gctagccttg ttctccaaga
     6481 gatagttcat ctcagcaatc ttgtattctg gcacattgtc caaacgctcc atcggggcat
     6541 ggaggaattt ctcacggtta atcacatcat ctgtccactg aacgatgacc tcatgcgcac
     6601 ccaaggcata agcttctttc acgattagat gtgccaattc acgttgctcc acatcaatag
     6661 agagagccaa agtgtgacca ggttgcacgt taattccgtt cgcaaccaac aatttcgcat
     6721 atttttctag attttcttta aaatttggta aaaccatcct gttttctctt ttctattttt
     6781 atttttcttt caaagcagaa aagattcctg ctaaagcaat agcacctgac aagagtgctg
     6841 tcgatagaag aattgtacga tcagcaagtt ctaattgata ttcaaatacc ttcttagccg
     6901 gcttatcttc cgcaaatatt cttagtttca tgagaacccc cttaaaatag attaaaataa
     6961 atcactgtga ctgcctgtcc tcagcaaatt taaaatcaat tccgacttat ctactttata
     7021 aaccaaaagc caatctggct gggtatggca ttcacgaact ccttgaaaat gcttggatgc
     7081 cgtcaatgaa tgatcacgat atctggcagg atgttctttt tcttgaacca gaaaattcaa
     7141 aacttcttct aataattctg ccttcaaacc acgcttcata gccaacttaa aatctttttt
     7201 aaactgttta tgataacgaa tcttaagcac gtaagtcctc catcaaatct gagactgatt
     7261 caaaagactg gctcatatta cgattttggt ctaaatccgt taacacttgg agcaacttcc
     7321 gattttcgtc tagtctaaca tcaaaaggta atccctgata ttgaattgcc tgacgaagga
     7381 aaatattaat agctgttgtc atatccattc ccagattact aaacacctgt tgggcctgct
     7441 ccttaacctc actatccaga cggatgctca tactcatctt tgacatactc tcaccctctt
     7501 tccatagact attttaacaa aaaagaaagc taatgtaaat ctattggata tacgttagcc
     7561 tcttctaata gattattaag caatttttta aaacaactca tcaaacaaac tcaactggtt
     7621 atcctctggc atatttccaa gaatacccat ctcatccatc ttttcaacca aggttgatga
     7681 gagtccacca cgcttgcgta gttctgtttt agagaggaat tctccctctt cacgcgcccg
     7741 caccagttgc ttggcaacgt tctctcccag accatccatt gctacaaatg gtgggataag
     7801 ggtatccccg tcgatgagga actctgtcgc ctgactacgg tagagatcta atttgccaaa
     7861 cttgaaacct cgttcccaca tctcattgac aatctcaaga gttgtataga gatcgatttc
     7921 cacattagag gcttcattgt tcttccgttt ttcagagatt tcttccattc tgcgcttgat
     7981 ggcctccaag cccgcaccca tggtcttgat atcaaaagcc ttagcacgaa tggagaagta
     8041 agcacagtag taataaatag gatggtgaac cttgaagtaa gctacacgca aggccatcat
     8101 aacgtaggct gccgcatggg ccttagggaa catgtactta attttcccac aggattcgat
     8161 ataccactct ggcaccttat tagccttcat ggcttcgata tagccatttc tctcctcttc
     8221 tgaaatcttt agccacaaac ccttacgtac ccgttccata atggtaaagg ccatcttagg
     8281 ttccagaccc gcatgcatga ggtaaaccat gatgtcgtcc cgacaaccga taacagtcga
     8341 taggtccgct attccttgct taatcagatc ctgagcattc cccaaccaaa cgtcagtacc
     8401 gtgggacaga ccagacagct gaagcaattc cgcaaaggtt gtcggatggg tttcgtctac
     8461 cattccacgt acgaaatttg ttccaaactc tggaatcccc aacatacccg taggcgttcc
     8521 aatttgttca ggtgttaccc ctagcacatc agtcccagaa aagagtgcca tcacgccttc
     8581 gtcatccata ggaattttat tagggtcaat accagacaaa tcctgaagtt ttcgaatcat
     8641 ggtcggatca tcatgtccca gtacatcgag tttgaggacg ttctcatcga tatcgtggaa
     8701 gttaaagtga gtggtctgcc attcagccgt gacatcatct gctggatact ggacaggcgt
     8761 aaaatcgtag acatccatgt agttcggaat aacaacgatt ccccccgggt gttggcctgt
     8821 tgtccgcttg acacccgccg ctccttgagc gaggcgttct acttctgcat cacgataaaa
     8881 cttgccataa tctcgctcgt aacccttgac aaatccatag gcagtcttgg cagctaccgt
     8941 accaaccgtt cccgcacgga aggcatattc ttcaccaaag atatcacgca catccaagtg
     9001 ggcgctaggc tgatcttctc ccgagaagtt caagtcaata tcaggaacct tatccccatc
     9061 aaaaccaagg aaggtctcaa acggaatatc ctgtccgttt ttactgagtt tgtgaccaca
     9121 gtttggacag tccttatggg gcatatcaaa tcctgaaccg tacgaaccat ctgtgataaa
     9181 ctcactgtac tgacactgac cacagacata gtgaggagag agaggattga cctccgtaat
     9241 cccaatcatg gtcgcaacga aactagatcc gacagaccca cgagaaccaa ccaaataacc
     9301 ccgttcatta gaacgttgca ccagcatctg cgatgccaga taaatcacag caaatccatt
     9361 ccccagtatg gatgttaatt ctttttcaat ccgcaaatca acaatatctg gcagcggatt
     9421 tccataaatc tcaaaagctt tcttataggt caactcagca actgtttctt cagccttgtt
     9481 gatgaaaggc gtatacaagt cacccttaac gacttcaacg ggttcaaata tttctgccaa
     9541 ggcattagtg ttttcaataa ccagtttacg agccagttcc tctcccaaaa aggcaaattc
     9601 atccaacatc tcattagtcg ttcgaaaatg agcctttgga agtggcgctg gttgggcatg
     9661 ttcaccatga ccgatagttc ggttaatcat cgcaccctgt cccaaactac ggacgataat
     9721 ttcacgataa atctcttctt ccggttcgat atagtgaaca tttcccgtag ccagaacagg
     9781 cttgccaagg cgatctccaa cctctatcaa actcttgata atggtctgga gttcctccat
     9841 atccttgacc tgctctttgg caatcaaggg cgcatagata gccggtggca tgacctcgat
     9901 aaagtcataa tacttggcca cctcaaccgc cgcatccaca ccttgagaaa cgaccacgtc
     9961 aaaaacttca ccctctgaac aggctgaacc taaaatcaag ccctctcgat gggcatctag
    10021 aaccgttctc ggaatccgtg gcactccttc aaaatacttg gtattagaca aggaaaccag
    10081 cttaaagata ttttttagac ctacctgatt cttgacatag atggtcgcat gcttgatccg
    10141 agcttttttg taagaatctg gactgattag atcaatgttg agtctagcta aatcggtcac
    10201 accatgtttt tctgctacct ctttgataaa gatgaaaagc agacgaccag tcgcttccgc
    10261 atcgtagttg gccatgtggt gatgttccaa ggccacacca aaacgcttgg tcaaaggccc
    10321 caaaccatgg cgtttatact caggatagag gtttctagca aactccagcg tatcaataac
    10381 tggctgacta atctttggaa gaccatgacg ctcataatta gcattcataa agccaacgtc
    10441 aaaggcagca ttgtgggcaa ctaggaccgt atccttgcaa aattcttgga attcttgcaa
    10501 aacttgttct agtggtttgg catttttgac atgatcatct gtaattccag ttaactctgt
    10561 agtaaaagct gacaagggat gcccaggatt gataaattca tcaaattcag caataacatt
    10621 ccccttgtac atcttagagg ccgcaacctg aatcaagtca ttatagatag ctgaaagtcc
    10681 cgtcgtttcc acgtcaaaga ccacgtaggt tgcttctgat aagtccatct ccacttcgtt
    10741 atagacgata gggacacggt cctccacgat attggcttct atcccataga tcagctggat
    10801 tcccgctttc ttagccgcct tatagccatg tggaaaggac tggacattcc catggtctgt
    10861 gatagcaacc gccttgtgtc cccacttagc agctgttgca acaatctctt cgacctctgg
    10921 caaagcatcc atagtcgaca tgttagtatg agcatgaaac tcaacccgac gctcaccttc
    10981 tggcatcaaa tccttccgct catagtgaac aacttcctgc agatcctgta cgttcatagt
    11041 caaatcgcgt gtgaagttat tcatctccac attccctcga actcggagcc aagaattctt
    11101 cttgatgagg tcaaacttct gggcctcttc ctcgttttta acccactttt gcatagaaaa
    11161 acttgaagta tagtccgtca ttttaaagtt gattaaaaca cgacctgttc tagtcacttt
    11221 ttgctccaca tcaaaaacaa ccccttcaaa taccagacga ttttcctctg tcgtcacttc
    11281 gatcatagga gtaatctccg ccttatccag cttgggttta gctgcagctt ttttcgcttg
    11341 aaaatcaaag actggtttct cttccgctgg aggaggtgcc atctgctcca gttgttccat
    11401 agcacggagc gcttcctcat tggcagcttg aacaatctgc tcattttcag catgaaaggc
    11461 ctcttcctgc tcttgggtca ggacatcatt cttctcgact tgacagttaa aagttggaaa
    11521 accaaacttt tcaagttgtt tggctaaatt aggaagatga ttcttcttaa aatgttcctt
    11581 atcaatcgct tcagatcctt caataaatag ctgattaccc tcagcacgaa cttgcaaatt
    11641 ttgataaagg gacttaaaac cttgactagc acatggacct tcagagaaag cctccctata
    11701 gtaggactgc aagagctgat ttgaaaattc ttgagaccga gccttagttt caaaaacagc
    11761 tttattgcct gtcttagaaa attcttcgct caaacctttc tttaattcta aaaagatttc
    11821 aatcggtaaa atattagaaa atacgaaatg aaactcccat accttactaa ttttatgaac
    11881 cacaactcgc tcaatattgg cctgtgctaa agcaggagcc tgtctcattt cagcaggcat
    11941 ccccaattga ttcatcaaaa tttcaaaact atttgacatt cattttcctc acattattct
    12001 tctactattt taccatattt agaggtatt
//
LOCUS       DAVLYV010000055        11893 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLYV010000055 DAVLYV010000000
VERSION     DAVLYV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.055"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(52..1101)
                     /locus_tag="VYO70_001490"
     CDS             complement(52..1101)
                     /locus_tag="VYO70_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1713703.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKIGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRSEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1221..3029)
                     /gene="glmS"
                     /locus_tag="VYO70_001491"
     CDS             complement(1221..3029)
                     /gene="glmS"
                     /locus_tag="VYO70_001491"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1713704.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNSRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDDAGQ
                     VVIDPAIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLSLLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(3221..4600)
                     /gene="bglA"
                     /locus_tag="VYO70_001492"
     CDS             complement(3221..4600)
                     /gene="bglA"
                     /locus_tag="VYO70_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1713705.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPDSPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYDNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            complement(4699..6552)
                     /locus_tag="VYO70_001493"
     CDS             complement(4699..6552)
                     /locus_tag="VYO70_001493"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1713706.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     SDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(6565..7824)
                     /gene="rseP"
                     /locus_tag="VYO70_001494"
     CDS             complement(6565..7824)
                     /gene="rseP"
                     /locus_tag="VYO70_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1713707.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(7846..8649)
                     /locus_tag="VYO70_001495"
     CDS             complement(7846..8649)
                     /locus_tag="VYO70_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1713708.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(8658..9416)
                     /locus_tag="VYO70_001496"
     CDS             complement(8658..9416)
                     /locus_tag="VYO70_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1713709.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            complement(9647..10111)
                     /locus_tag="VYO70_001497"
     CDS             complement(9647..10111)
                     /locus_tag="VYO70_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1713710.1"
                     /translation="MAAGSVRNFIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLE
                     KKLREDFPQYQWELKNQVYMHQHSPHTAFYTSSRDAMSKYPERCTAVGLRLNEESDFE
                     LYAPYGLEDILNFQVRPTPHFLENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(10200..11171)
                     /gene="ruvB"
                     /locus_tag="VYO70_001498"
     CDS             complement(10200..11171)
                     /gene="ruvB"
                     /locus_tag="VYO70_001498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1713711.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            11332..11424
                     /locus_tag="VYO70_001499"
     CDS             11332..11424
                     /locus_tag="VYO70_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1713712.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         3477 a         2807 c         2235 g         3374 t
ORIGIN      
        1 taattttcgt acaatatttg ttgtcctaat aaacttgatt aggatttttt attaagcctc
       61 tttcatagca aaataagctc gtactttggg tgcaacttgt gttccgaaga gttcaatagc
      121 tctcagaacc tggtcatgag gcatagaacc aagcggtaga tgaagcatga agcggtccaa
      181 tcctaaatcc tctatcatgc gaatcaattt ttcggccacc tgatctggat tgccaacaaa
      241 catggcgcca tttggcccta cctgctccaa atattgctca taacgcaatt cctgccagtg
      301 cggacggtct ttggaaatag catccactac ttgcttagtc ggatggaaat aatctttcac
      361 cgcctgctca ccatcttccg caatccaccc ccaagaatgg gctcccactt tcaagtcttt
      421 gtcagcatgg cccgcttcgc ttccaatctc acgataagcc tgaatcaact ttttaaaata
      481 acgtggatta ccaccaataa tagcatatac aatcggtaga ccagcctgag caatcttcac
      541 tgttgattcg acatgaccac ctgtagctat ccacaagggc aatttgtcct gaactggacg
      601 aggataaact tctttaccag caatcgtttg agtcaatcga ccttgccagt ctaacttggt
      661 cttttcattg actaactgaa gcaagtctaa tttctcatca aaaagagagt cgtagtcttt
      721 caagtcataa ccaaacagag ggaaagattc cgtgaaagag ccccttccag ccataatctc
      781 cgatcgtcca tttgacaaag catcgatagt ggcatactgt tggaacaaac gaatcggatc
      841 catgcttgac agaatgctga ctgcactggt caaacggatt ttcttggtat tgactgcccc
      901 agcggccagg acaatctctg gggctgatac tgcaaaatcc gcccgatggt gctcgccaat
      961 cccatatacg tccaaaccaa tcttgtcagc cagctcaatc tctgccacca actggcgaat
     1021 gcgttcagca tgactgtaag tttgtccagt cccttcaagc tccgttattt ccccaaatgt
     1081 tgaaattccc aattctacca ttgtgattct ccttgtctat ctctgtcctt caatttgaaa
     1141 aattattcta acacgaatct taagtacaag caaccaattt gctcacaaga aaaaggccta
     1201 gataactaga cttttttagc ttattctacc gttactgact tggcaaggtt acgtggtttg
     1261 tccacatcga ggccacggtg gagggttgca aagtaagcga ctaattgcgt tggtacgacc
     1321 attgaaattg gtgagaggta tggatgtacg gtcgtaagga cgatatcgtc ggtatctttg
     1381 gctacattct cttctgcgat agtgaggact ttggcaccac gggctgcgac ctcttggata
     1441 tttccacgag tatggttggc aagaactgga tctgacaaga gagacaagac aggcgttcct
     1501 tcttcaatca aggcaatggt tccgtgcttg agttctcctg ccgcaaaacc ttcacactgg
     1561 atataagaaa tctctttgag tttgagactt gcttccatgg ctacgtagta atcttgacca
     1621 cgtccgatgt aaaaggcgtt acgagttgtt tcaagaagtt cacgaacctt ggcttcaatg
     1681 gtttctttct ctgaaagagt tgattcgata gactgagcta cgattgacaa ttcatgaacc
     1741 aggtcaaagg cttgcgcttt agcattacca tttgcttctc cgactgcttt tgcaaggaag
     1801 gcaagggctg cgatttgcgc tgtataggct ttagttgatg ccacggcaat ttcaggacct
     1861 gcgtgaagga gcatggtata gttggcttca cgtgagaggg ttgaacctgg aacgtttgtc
     1921 actgttaagc ttggaattcc catttcatta gccttgacca aaacttgacg actatccgct
     1981 gtttcaccag attggctgat aaagatgaag agtggtttct tgctgagaag tggcataccg
     2041 tagccccact cagatgagat tccaagttca actggtgtat ctgtcaattc ttccaacatt
     2101 ttcttagaag caaatcctgc atggtaagat gttccagctg caaggatgta gatgcggtct
     2161 gcgtcttgaa cagccttaat gatagcagga tcaatcacta cttgaccagc atcatccgtg
     2221 taggcttgaa tgagtttacg cataacagtt ggttgctcat caatttcctt aagcatgtag
     2281 taaggataag ttcccttacc gatatctgac aagtcaagtt ccgcagtata gctagcacgt
     2341 tcacgactgt taccatcata gtcttgaact tccacgctat cagccttgac gattaccaac
     2401 tcttggtcat gaatttccat gtattggttg gtttcacgaa tcatagccat agcatctgag
     2461 cagaccatat tgtagccttc cccaagacca atcaaaagtg gagatttgtt tttcgctaca
     2521 tagatgacat ctggattttc agagtcaatc aaggcaaagg catatgaacc acggataata
     2581 tgaagagctt ttttaaaggc ttcaagaact gagagaccgt cttcttccgc aaattttcca
     2641 atcaaatgta cggcgatttc cgtatctgtt tgccctttga agtggtgccc agcaaggtat
     2701 tcttctttaa tttcaaggta gttttcaatc accccattgt gaaccaagac aaaacgttct
     2761 gtctcagagc ggtgtgggtg agcattgtct tccgttggtt tcccatgagt tgcccaacga
     2821 gtatgtccga taccagttgt tccctcaaca ccagctgtct tggcagacaa ttctgcgata
     2881 cgaccaacag ccttcaccaa atggttatca gcaccatcta ggacaaaaat tcccgcagaa
     2941 tcatagccac ggtattcaag cttttcaagc ccttgaatca aaatatcagt tgcatttgtg
     3001 tttccaacaa caccaacaat tccacacata gtatatacga cacaggcaag ctgtgctttc
     3061 tccttaaaat tggtatagtc taattcatct tttatagaat cagcaaaaac agtatatact
     3121 tgtttctttc acttgtcaag agtaaaaatt ggtatagttc aaattaagct cctgtaagca
     3181 aaaaactctg accgattggg ataatcagtc agagtccttt ttaaaatcca ttattatcgc
     3241 ttaattcttt gaaccagtgg cctgatttct tcagacgacg ttcttgcgtt tccaagtcta
     3301 attcgaccaa accatagcga tttttatagc tgttgagcca tgaccagcag tcaataaagg
     3361 tccaaatcaa gtagccctta cagttggcac catcttcaat ggcacggtga agttcacgaa
     3421 gatgaccttt tacaaagtca atacggtaat catcttgaat cattccatct tgacggaatt
     3481 tttcttcccc ttcaacaccc ataccattct cagtcaacat ccattcaata ttgtcataat
     3541 tttccttgat attttgggcg atgtcataaa tcccttgctc ataaatctcc caaccacggt
     3601 gagaattgat tttacgtcca ggcatcacat aaggctcgta aaaatgttct ggtaagagtg
     3661 gactatctgg atgcttagca aatcgaggag ccataacacg caaaggttga tagtagttca
     3721 caccaaggaa gtccaccgta ttatcacgaa tgagttccaa ctcttcctct gtagcatcag
     3781 gtaaaagacc gtgttcatgc aagatttcta ccaactcctg tggataagtc cccaagacag
     3841 atggatctaa gaaagattgg gcctgaaaaa gggccgcaat acgagctgcc ttgacatcag
     3901 caggatgctg gctacgtgga taagccggtg tcaagttgag gacaatccca atcttggaat
     3961 caggcaaaag ttcatggcaa gccttaacag cccggctgct ggccaattgt gtatgatagg
     4021 ctaccttaac agctgcctct gcatccacct tatgtggata atgggcatca taaaaataac
     4081 caaattctac aggaacgatg ggctcgttaa aggtaatcca ttgatccact aaatctccat
     4141 aagtctcaaa acaaaaacga gcatagtctt cataggctga gactgtcgcc ttattttccc
     4201 aaccatcacc atcctcttga agggcaaaag gtaaatcaaa atgatagaga ttgactaaca
     4261 gacgaattcc tttagcctta atagcctcaa agaccttacg ataaaaatcc acaccttgag
     4321 ggttgacttt tccacagcct tgtggaaaaa tccgtgacca ctgaatagaa gtccgaaagg
     4381 ctgtgtgacc agtctctaac aaaagctcaa tatcccgctc ccaattttca taaaaagtcg
     4441 atgtcttatc tgaaccaatc ccattatagt aacgatttgg ctccacttgg aaccagtaat
     4501 cccagagatt gtctccctta ccgtcaccag ctacacgtcc ttctgtctgc ggtccagaag
     4561 tagaggatcc ccagacaaaa tcctttggaa atcttagcat acatttacct ctttatctac
     4621 tcatttctcc cattatacag aaaaaacaag gtaaaaacta gttacatttt ttccttgttt
     4681 ttcttctgat tatagttttt atttcttgct taggatttca agcgtttcaa gcacgttatc
     4741 tgcatgaacc tcaatggtgt caccagttgc cttgatctta acttctacaa tgccatcggc
     4801 cgctttttta ccaacagtga tacggattgg aagaccaatc aagtcactat cgctaaattt
     4861 aacaccgaca cgttcgttac ggtcatctgt caagacttca taaccagctc ccatcaagct
     4921 tgcttcaagt ttttctgtca aggcttgcgc ttcttcatcc ttgacattaa cagtaatcaa
     4981 atgcacatca aacggtgcca attctttagg gaaattgatt ccccaagcgt aacggtattc
     5041 accttttggc gttttgttaa caaagaggcg agcgtgttgc tccatcactg ctgaaagaag
     5101 acggctgaca ccgataccgt aacatcccat gatgattggc acagcacgac cattttcatc
     5161 caagacatct gctcccatgc ttgctgaata gcgagttccg agtttgaaaa tatgaccgat
     5221 ctcaatacca cgcgcaaagt taaggacacc ttgtccatct ggggaaattt caccctcacg
     5281 aacttcacgg atatccacat attctgcagt aaaatcacgg cctgggttca caccagtcaa
     5341 gtggtagcca tcttcgttag caccgacaac tgcattgcga acatcttgca ccttacgatc
     5401 tgcaataatt ttaatattct ctggcaaacc aactggtcca agtgaaccaa atcctgcttg
     5461 aacaacattc gccacttctt cttcgctagc aacgtcaaag aaatctgctc ccaagtgatt
     5521 tttcaacttg acttcgttga gttggtcact tccaactaga agggctgcaa caagctcgcc
     5581 atctgcaatg tagaagaggg ttttaatcgt ttgttcttct ggaacattga ggaaggctgc
     5641 aacttcatca attgatttaa catctggcgt tgcaacacga gtaacttctt cttcagcgac
     5701 aacacggttg cttggtttgt actcgtttgt tgccatttct aagttagctg catagctaga
     5761 ctcacttgag taagcaatgg tatcttcacc agagactatc catttgagca attctgcctt
     5821 gatttcttct tgcacttctg caggaatttc gtcaaatgag gcaactgact tgtccaagac
     5881 aacccagcgg tcaaggtctg tacgagcaga tgtaatggcc ataaattctt ggctatcctt
     5941 accacccatg gctccaccgt caccaataat agccttgaag tctaaaccac tacgagtgaa
     6001 aatacgctca taggctgctt tgtactcatc ataaacacta tccaaactat catagttagc
     6061 gtggaaacta taagcatcct tcatgataaa ctcacgtgta cgaagaagtc cattacgcgg
     6121 gcgtttttca tcacgatact tgggctgaat ttgataaagg ttgagtggca attgcttgta
     6181 agatttaaca gaatcacgga caatagctgt aaaggtttct tcgtgagttg gacctaagat
     6241 aaagtctgat ttttcacggt tttttagttt gtaaaggtct tcaccatagg tttcgtaacg
     6301 acctgattca cgccacaatt ctgcactaag aagggcagga gccaacatct caacagcacc
     6361 aatcttttcg aattcttggc gcatgatgtt tttagctttt tcaatcacac ggttggcaag
     6421 tggtagataa gaataaacac ctgctgaaac ttggcgaaca taaccagcac gcaacataag
     6481 agcatggctg ataacttgag catcgcttgg catttcgcga agcgttggga taggcatttt
     6541 actttgtttc ataatattcc tcgattatct aaaaaagagt cgcataatgt cattccaagt
     6601 cacagcaatc atcaagacaa ccatgatgac cactccggcc aaggtgacat aggtttcaat
     6661 ttcttgtttc aatggtttgc ggcggatggc ttctaggata ttgagcacaa tcttaccacc
     6721 atccaaggct ggaatcggaa taagattaaa aatcccaata ttgatggaaa tcattgccaa
     6781 gaagtacaag atattttcaa ttccattttt agcagcatca ctacttgcct taaagatagc
     6841 aacaggtcca cccaacttgt tcaaatccgg ttggaaaatc agatttttca gagctgagag
     6901 aattcggaga gctgagtcag cagcagttgt aaaaccacct acaaacatgg atagaaaatc
     6961 tgacttaacc cccggttgaa cacctagaag gtaacgacct tgactatctt cgggtgtaac
     7021 agtgacttgt ttgtcactcc ccttttcaga aatagtcaca tccaaagtcg gtgccgtctt
     7081 atctttggtt tctgtttcca cagcttggat caagctttcc cagttgctaa cctcatgtga
     7141 gccaatcttg gtaatttgtg ccgtttctgg tactcctacc ttggccaagg caccttgggg
     7201 catgatatgg aactgattgg tatcaacatc tctgacacca ccctgcataa agattaaaac
     7261 ccaaaaaaca acgacaccta agataaaatt gttcatagga cctgcaaaat tggtaatcag
     7321 tttgccccag atagtcgcat tttgatattg aacatctaaa ggtgcaatcc gaacctcagt
     7381 accatctgct tccacaaccg ttgcatcgtg atccactgca aatgtttttt cttcttccag
     7441 aaccaatcct ttgataaaga gcttgtcttc aaaatcaaac tgggtcacct gcatagggag
     7501 ggctgtttga tccaattttt tacctgagag attgatgcgt ttaaccttac catcatcagc
     7561 aagtgtcaaa ctaacaggcg ttcctgtctt gatttcagtt gtatcatcac cccaaccggc
     7621 catgcggaca tagccaccca gaggcaagat tcgaatggta taggccgttc catccttgcc
     7681 aatgtgagca aaaattttag gtcccatacc gatggcaaat tcacgtacta aaatccctga
     7741 tttcttggca aagtagaagt gcccgaactc gtgcaccact acaataatcc cgaaaaccag
     7801 aataaaggtt aaaattccga gcatagcgtt tcctccgtct tttgattaaa agagtccaaa
     7861 taagtgcatg attggaaata caagcaacat actatcgaaa cgatccaaaa caccaccatg
     7921 tccagggata aatttcccag aatccttaac accaaaatga cgtttgatcg aactttctag
     7981 taaatcacca aattgtccag caatgctaaa gaaaatagca aagactgaca tcttgtaaat
     8041 tccatatgga agagcaactg tactgtcaac tatcataaag ataatggtta ctaaaattgc
     8101 tcctaaaata ccacccaagg caccctcaag ggttttatta ggcgataccc ttggtgctaa
     8161 ctttcgtttc ccatagttca tcccaacaag ataggcacca ctgtctgtcg cccagacgat
     8221 acacaaggct aagagagcct tgtccaaacc tgcaacacga gcatctagta aagcattaaa
     8281 tccaaagccc acgtagaagc tcatagcaag agggaaaacc gcatcctcaa tcgtataaga
     8341 cttgctaaaa acggtcgttc ctaacatgat tgaaatcaaa acactatagg caaccacatt
     8401 cccatcaact ggcaaaaaag tcaggtaatt ctccaaggga atggtcaatg caaaggttgc
     8461 aaagagggtc aagaggccct ccatcgtcat ggtctctaga cctctcatct tcaaaagttc
     8521 atgcatggct agcatggcta tgattccgat tgctatctga agcaagaggc ccccaatcat
     8581 taaaattggt aggaaaatag ccagggcaat ccctgcaaac aaggttcttt tctgtaaatc
     8641 ctgggtcata tttcctccta aactcctcca aatcggcgat gacgacgatt ataggcaaga
     8701 atagcttcct gcaaggccgc ttcgtcaaaa tcaggccata aggtgtccgt aaaataaagc
     8761 tcactatagg ctccctgcca tggaaggaaa ttgctcaaac gtaattctcc actagtacgg
     8821 ataatcaagt ctgggtctcg taagtcctta ggcaaatgct gggtaaagag atagttacca
     8881 atcaattcct ctgtgatgtc acctggattg attttggcat ctaaaacatc ctgggaaatc
     8941 aacttaagcg cctgtgtaat ctcagcacgt ccaccatagt taagagcaaa attaagaatc
     9001 aatcctgtgt tgttcttagt caattcctca gccttggtta aagcttcaaa ggtttgctta
     9061 ggcaggcggt ctgtctcccc aatcatttga atcttaacat tattcgcatg tagttccggg
     9121 acataattat cataaaactc tactggcaag ttcatgataa acttgacttc ctgatctgga
     9181 cgggtccagt tttccgtaga aaaagcatag accgtaataa ccttgacgcc cagtttgttg
     9241 gctgccttgg tcacggtttg caatgcttcc atgcccgcct tatgtccaaa aactcgcggt
     9301 tgcatacgtt ttttagccca acggccattg ccatccatga tgatgccgat atgagcagga
     9361 acctgtgtcg gaacctctac ttccacagcc ttatctttct taaaaaatcc aaacatgatc
     9421 ttattcctat tcaaaaatct atcgtttcat tataccatat ttccccattt tcttctatca
     9481 ctaagctatt tattctcagg caccatgccc atttttcaaa aaaataagcc gcctgattgg
     9541 gcgactttat ttttataggg agattattat gaaaaagttt taggagttta agttaaggtc
     9601 ttcttaactt atgaacttag tgtacactcc ctagcttaaa gtttccttaa gtatttttaa
     9661 aaatcaaatt tttccatttc tcctgccaat ttttcttgga taaacgtgtt tgatagagtt
     9721 ccattcggtc ttcattttct aagaaatgag gagttggacg aacttgaaaa ttcaaaatat
     9781 cctccaaacc ataaggtgca taaagttcaa aatctgattc ttcattcaag cgcagtccaa
     9841 ctgccgtaca ccgttctgga tacttactca tagcatcacg agaactggta tagaaagcag
     9901 tgtgaggact gtgctggtgc atatagacct gatttttcaa ttcccactgg tactgaggaa
     9961 aatcctctct cagctttttc tccagtaata aggtttcctc ataagaaaaa tctggatcaa
    10021 agaaaatcac atctatatct gtttcatgat caaaagggga tttgtctgac aaaagattcc
    10081 agatgaaatt tctgacagaa cctgctgcca actacgagtc tttcaaacca aggtctcgga
    10141 tgatcgtcag aatggccatc atatctggat tttctctaaa agcctctaag atttcttgct
    10201 tatttttcac tgtattcata acctaagtgc tcatatgcct tagcagtcgc cacccgtcca
    10261 gaccgtgtcc gcatgataaa acctttttga atcaagtaag gctcatacat gtcttcaact
    10321 gtctcacgct cttcggcgat attgacagaa agagttccta gaccaacagg tcctccactg
    10381 tacatctcaa tcatggtacg aaggattttt tgatccacat agtccaaacc ttcatggtca
    10441 acatccagca tagtcaaagc cttatcggta ataatatcat cgattacccc attccccatt
    10501 atctgggcaa aatcgcgcac gcgcttgagg agacgattgg caatacgagg ggttccacga
    10561 ctacgtaggg ccaactcaga tgctgcctca tgagtgattt ccatctcaaa aatatctgcc
    10621 gtccgctcga caatttctgt caagtcagca tgggcataat actccatatg acctgtaatc
    10681 ccaaaacgtg cccgtagtgg atttgagagc ataccagccc gagtcgtcgc accaatcaag
    10741 gtaaaaggag gcaactccaa atgaacactg cgactgcctt caccagcccc aatcataata
    10801 tctatgtaga agtcctccat agcactataa agcacctctt ccactgacat tggcaaacga
    10861 tggatctcat caataaaaag gacatcccca ggctctaact cattcaaaat agctaccaga
    10921 tctccggctt tttcaatgac tggacccgac gtctgcttaa gattgactcc cagttcgttg
    10981 gcaataacaa aggccatggt cgttttcccg agacctggag gcccaaataa gagcacatga
    11041 tccagcgctt catcccgcat ttttgcagct tcaataaaga tttgtagctg gtccttgacc
    11101 ttatcctgtc cgatatattc acgtaaatac tgaggacgga gcgtgcgttc tactaactcc
    11161 tcatccccca ttatctcatt atctaaaatt ctacccatgg ctctattata tcaaaaaacc
    11221 aagccacaaa caaaaaagcc acctgattgg gtgactccta agtttagcac ttatgtggta
    11281 taatattata cggcacttct acaccgccta cgaaaggagg tgagatagcc catgatggaa
    11341 ttagtactca aaactatcat cggaccaatt gtggtcggtg tcgttcttcg tatagtcgat
    11401 aaatggctaa acaaggacaa atagtgtcaa aaaagacctc aagcttattt ggtcgtgagc
    11461 ttggggtctt ttctagccta tgatatagaa ctagtactca attccttttt attatcccat
    11521 agttcacgaa ttttgtcaaa ggtttacatt ttcttcaacc gctgtacgac aagacggtta
    11581 aaattaagag aacgttaggg attctatcaa tttcatagaa attttgattt cgtaaacgaa
    11641 gagacaatct tacatgtcac ttttcattta atacgccact actagacaag caaaatcatt
    11701 attacagtag ttccagtcct tcaattaaca gtcacttaca atcaaattga gtttgaacta
    11761 gctgaagcga ccacagacct atttcttagt catattcgct aaaaaaatcc cctccaaaat
    11821 ctcaaaaagt ccccgccaat tccccgacca aaatccgaaa aataccgaaa aatatcgaaa
    11881 aattattttt aga
//
LOCUS       DAVLYV010000056        11850 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLYV010000056 DAVLYV010000000
VERSION     DAVLYV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11850
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.056"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            118..771
                     /locus_tag="VYO70_001500"
     CDS             118..771
                     /locus_tag="VYO70_001500"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1713713.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            781..1245
                     /locus_tag="VYO70_001501"
     CDS             781..1245
                     /locus_tag="VYO70_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1713714.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECATREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1258..2526
                     /locus_tag="VYO70_001502"
     CDS             1258..2526
                     /locus_tag="VYO70_001502"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1713715.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLS
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRFVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2540..2800
                     /locus_tag="VYO70_001503"
     CDS             2540..2800
                     /locus_tag="VYO70_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713716.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2811..4172
                     /gene="pdrM"
                     /locus_tag="VYO70_001504"
     CDS             2811..4172
                     /gene="pdrM"
                     /locus_tag="VYO70_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1713717.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPIILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSPLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKC"
     gene            4326..5294
                     /locus_tag="VYO70_001505"
     CDS             4326..5294
                     /locus_tag="VYO70_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1713718.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5310..6302
                     /locus_tag="VYO70_001506"
     CDS             5310..6302
                     /locus_tag="VYO70_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1713719.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKD"
     gene            6520..7563
                     /locus_tag="VYO70_001507"
     CDS             6520..7563
                     /locus_tag="VYO70_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1713720.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7627..9312
                     /gene="lpdA"
                     /locus_tag="VYO70_001508"
     CDS             7627..9312
                     /gene="lpdA"
                     /locus_tag="VYO70_001508"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1713721.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9376..10365
                     /locus_tag="VYO70_001509"
     CDS             9376..10365
                     /locus_tag="VYO70_001509"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1713722.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10436..11506)
                     /gene="xerS"
                     /locus_tag="VYO70_001510"
     CDS             complement(10436..11506)
                     /gene="xerS"
                     /locus_tag="VYO70_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1713723.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSALENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3511 a         2218 c         2661 g         3460 t
ORIGIN      
        1 attcacgtat tcaaatcgta tatagtcgat ttattaaatc tttagataaa ttgtaaaaag
       61 tggtttccat agccactttt tgctataata gaggcagtaa aaatgaagga gtcagctatg
      121 gaacactcgt cttggcatgc tttgattaag gcgcaattac ctgagggtta tttcgggaaa
      181 atcaatcagt ttatggagca ggtctattct caggggatta tttatccacc caaggaaaag
      241 gtttttcagg ctctcttgac aacactgctt gaagaagtta aggtggtaat tctagggcaa
      301 gacccctatc acggaccagg tcaagcgcag ggcttgagtt tttctgtacc tgactctatc
      361 ccagctccac catccttgca aaatatcttg aaagaattgt cagatgatat cggagttaag
      421 aaatctcatg atttgacagc ttgggctgag caaggagtct tgcttcttaa tgcttgtttg
      481 actgttcctg ctggacaggc caatggtcat gctggtcaaa tctgggagcc ttttactgat
      541 gctgtgattc aggtggtcaa tcatctagat agaccagtcg tttttgtact ctggggagct
      601 tatgcacgta agaagaaggc cttagttacc aatcctcatc acttgattat cgaatcagcc
      661 catccaagtc ctttgtcggt ttatagagga ttttggggtt ccaagccctt ttccaaggcc
      721 aatgcattct taaaagagac aggacaagag ccaatcgatt ggcttagata aggagaaaat
      781 atgcctcagt tagcgacgat ttgctacatt gataatggga aagaactgct catgctccat
      841 cgtaataaga aacccaatga tgtccatgaa gggaaatgga ttggtgtggg tggtaagcta
      901 gagagaggag agacgcccca ggaatgcgcg acgcgtgaaa tccttgaaga aacagggctc
      961 aaagccaagc cagttctaaa aggtgtcatc acttttcctg aatttacacc agatttagac
     1021 tggtacacct atgtttttaa ggtgacggag tttgagggcg acttgattga ctgcaatgag
     1081 gggacgctag aatgggttcc ctatgatgag gttttgagca agccgacttg ggaaggtgac
     1141 cacacctttg ttgagtggct tttagaggat aaacccttct tttcagccaa gtttgtttat
     1201 gatggggata aattgttgga tacccaagtt gatttctatg aataaaggag aaagcagatg
     1261 ctactaatca aaaatggtcg tgtaatggat cccaagtctg gtttggatca agtttgtgat
     1321 gtcttagttc aagatgggaa aattatcaaa attgcgcctg agatcacgga agaaggagca
     1381 gaaacgattg atgctactgg tcttgtggtt gctcctggtt tggtcgatat tcatgtgcat
     1441 ttccgtgaac ctggtcaaac acataaagaa gacattcata ctggtgccct agcagccgct
     1501 gcaggtggtt ttactactgt cgtcatgatg gctaatacca gtccaaccat ttcagacgtg
     1561 gagactttgc aagcagttct ccagtcagct gccaaagaga agattaatgt caagacagtt
     1621 gcgaccatta ctaaaaactt taatggtaaa aacttgactg actttaaggc actcttagaa
     1681 gctggtgcgg ttggtttctc tgatgacggt attccgcttg agagcagtaa gattgtcaag
     1741 gaagccatgg aggaagccaa aaaactcaat acctttatca gccttcatga ggaagatcca
     1801 ggtttgaacg gtgttcttgg ctttaatgaa aatattgcta gagaacattt ccatatctgc
     1861 ggtgctactg gggtggctga gtacgctatg atggcgcgtg atgtcatgat tgcctatgca
     1921 actaaagccc atgttcacat ccagcatttg tctaaggaag aaagtgttaa agtagtggag
     1981 tttgctcagg ggttaggtgc agaagtcaca gcagaagtag cgccacagca tttctctaag
     2041 acagaagcac ttcttttaac acaaggtagc aatgctaaga tgaatccacc gcttcgtttg
     2101 gaatcagacc gtcgtgccgt tatcgaaggt ctcaaatcag gtgtcatcac agttattgcg
     2161 actgaccacg cgcctcatca tgtagatgaa aaaaatgttg aggatattac caaagcgcca
     2221 tctggtatga ctggcttaga aacatccctg tctctcagct tgacctattt agtagaagct
     2281 ggtgagttga gcttgatgga attacttgaa aaaatgacat acaacccagc caagctttac
     2341 aactttgaag caggttactt ggctgagaat ggtccagcag atatcactat ttttgatgcc
     2401 aaggctgacc gctttgtgga ctcccatttt gcttccaaag cagctaattc accattcatc
     2461 ggtgaaacct taaaagggca ggttaaatat accatctgta agggacaaat cgtctatcaa
     2521 gcttgatagg agttagaata tgaattattt tcgaaaacgt agggagagac aagcgaaatc
     2581 taatagtgga atttactgtc ccgcagctaa tcgttccaaa attcaatatg aaactaaaga
     2641 aaaagctgac catgcggttc aatatgataa cggaggttta gttagaagct attattgtcg
     2701 tacatgtgcg tgttggcata ctacttcaaa atctaataaa cctccgctaa gtttgaaaaa
     2761 ctatggtctg aaactctttg gtttagataa atcagaatag aaagagaagt atgtataaga
     2821 caaagtgttt acgagagaag ttagtattat ttttaaaaat tttcttccca atcctgatct
     2881 accaatttgc caattattct gcctcttttg ttgatactgc aatgacaggt caatacaaca
     2941 ctatggactt ggctggtgta tctatggcaa ccagtatctg gaatcctttc tttacatttc
     3001 taacagggat tgtgtcagcc ttggtgccta tcattggtca ccatcttggt cgaggcaaaa
     3061 aggaagaagt tgcgtctgat ttttaccaat ttatttattt ggccttgggc ctatctgtgg
     3121 tcttgctggg gatggtactt ttcttggcac caataatctt gaatcatatt gggttagaag
     3181 cagcagtagc ggcagtagcg gttcgctatc tttggttttt atctatcggg attatcccct
     3241 tgttgctctt tagcgtcatt cgttccttgc tggattcgct gggcttgacc aaactgtcca
     3301 tgtacctcat gcttttgtta ctccctctca atagcggatt taactatctc ttgatttacg
     3361 gtgcctttgg tgttccagaa ctgggagggg ctggtgctgg tttaggaaca tccttggcct
     3421 actgggtctt gcttgggatt tctgttctgg ttttatttaa acaggagaag ctcaaagcct
     3481 tacaccttga gaaacgaatt ccacttaata tggataaaat taaggaagga gttcgtttag
     3541 gtctgcctat tgggggaact gtcttcgcgg aagtggctat cttttcagtg gttggcttga
     3601 ttatggctaa gttttcgccc ttgattatag ctagtcacca gtcagctatg aacttttcaa
     3661 gtcttatgta cgcctttcct atgagtatct catcggctat ggctattgtc gtttcctatg
     3721 aagtgggagc caagcgattt gatgatgcga aaacctatat tggtctagga agatggactg
     3781 ccctcatttt tgcggccttc accttaacct tcctttacat ttttagggga aatgtggcca
     3841 gtctttatgg taacgaccca aaatttatcg atttgacagt gcgtttttta acttatagtc
     3901 ttttcttcca gttagcagat acctttgcgg cgccgcttca gggaattttg cgggggtata
     3961 aggatacagt tattcctttt taccttggtt tgcttggtta ttggggcgta gcaatccctg
     4021 tggctacgct atttgattcc ctaacagatt ttggagctta ttcttactgg atcggcttga
     4081 ttattagttt gattgtgagc ggggcgctct accgttggcg tttaactgtg attatgaaga
     4141 gatttgaatc tttagcaaaa tccaaatgct aaaaagtttg tgaaaaaata ttcttgacaa
     4201 gaaaatccct tgttttcacg gggttttctt ttttattttg tgaaatttct tttagaaaaa
     4261 ctttgacagt atttgccaaa agcggtaaaa tagtaaggta agaagaaagt tagaaaggca
     4321 agaagatgtc aactttagat aaaaatcttt tgctagaaat gttccgtaag atggaagaaa
     4381 tccgtcgcat ggacttaaaa attgcacaat tagtaaagaa agggaaagtg ccaggaatga
     4441 cgcacttttc tgttggtgaa gaggcagcta acgtgggggc tatgttagct ctcaatccag
     4501 atgatctgat tacctcaaac caccgtggac acgggcaagc tattgctaaa gggattgacc
     4561 tcaacggaat gatggctgaa atccttggta aatacactgg aacctgtaaa gggaaaggtg
     4621 gatctatgca tatcgctgac cttgatgctg ggaaccttgg tgccaatggt atcgtaggtg
     4681 gtggtatggg gatcgctgtc ggtgcagccc tcagtcagca aatgcaaaat accggtaaaa
     4741 tcgttgtctg cttctttgga gatggtgcga ccaatgaagg tgttttccac gaagcagtga
     4801 acatggcttc tatctggaac ctgccagtca ttttctattg cattaacaac ggttacggta
     4861 tctctgcgga tatcaagaaa atgaccaata tagaacatat ccatcaacgt agcgccgctt
     4921 atggaattcc tggaatgttc atcgaagacg gtaacaatgt catcgatgtc tatgaaggat
     4981 ttcagaaagc tgtggaccat gttcgcagtg gcaatggtcc agtcttgatt gaaagtgtaa
     5041 cttatcgctg gctcggtcac tcatcatctg accctggtaa atatcgtacg cgtgaagaag
     5101 tggaattgtg gaaacaaaaa gatccaatcg aaaacctccg caattacctt attgaaaata
     5161 acattgcaag tgccgaagaa ttggaagaaa tccaagcgca agtaaaggaa gcagtagaag
     5221 cttctgttaa atttgcagag gaaagcccat tcccaccgct tgaatcagcc tttgaagata
     5281 tttacgcaga ctaaggagaa agagataaaa tggaaacaaa aacaatgtcc ttccgtgaca
     5341 ccattatcct tgctatgtct gaggaaatgc gtcgcgatga aaatgtgttc ttgatgggag
     5401 aagacgtcgg tgtcttcgga ggagacttcg gtacttctgt tggaatgctt gaagaatttg
     5461 gtccagaacg tgtccgtgac tgtccgattt ctgaagctgc catctctgga gcagcagcag
     5521 gagcagccat gacaggactt cgtccaatcg tcgatatgac cttcatggac ttctctgtta
     5581 ttgccatgga caatatcgtc aaccaagctg ctaaaacacg ttacatgttt ggtggtaaag
     5641 gtcaggttcc aatgactgtt cgttgtgcag ctggtaacgg agttggctct gccgcccagc
     5701 actcacaatc tctagagtct tggtttactc acattccagg acttaaggtt gtggcacctg
     5761 gaacacctgc ggacatgaaa ggcctgctta agtcttctat ccgtgataac aacccagtta
     5821 ttatacttga gtacaagtca gaatttaacc aaaaagggga agtgccagtt gatccagact
     5881 acacaattcc acttggggtt ggtgaaatca aacgccaagg gacggatgta acagttgtta
     5941 cttatggaaa aatgcttcgc cgtgtggttc aagctgctga agaattagca gaagaaggaa
     6001 tttcagttga aattgttgac ccacgtaccc ttgttccgct tgataaggat atcatcataa
     6061 actcagtgaa gaagactggt aaggttgttc tggtcaacga tgcccacaaa acaagtggct
     6121 atatcggtga aatttcagct attatttcag aatcagaagc atttgactat ctagatgcac
     6181 caatccgccg ttgtgcagga gaagatgtgc cgatgcctta tgcacaaaac ctagaaaatg
     6241 caatgattcc aacagttgaa agtatcaaag atgcaatccg aaaaacttat aacaaagact
     6301 agagttacat aagataagtt atgtaaataa aacaaagctg acgagaaact ttgtatcttt
     6361 taggtgcaga gctcttcgtc attaatatct tagattagag cacgggctaa aagctcggaa
     6421 aaaagataaa tctccttggc ttcatcgaag ccatcgtcga tttcctattt ttcagtcgct
     6481 tttgacgccc tttgtatcat aaatagaatt ggagaggtca tggctgatga caagctaaga
     6541 gcgactcctg cagctagaaa gttagcggat gatttaggga taaacctcta cgacgtttct
     6601 ggctcaggtg caaacggtcg tgtccacaaa gaagacgtgg aaacttataa agacacaaac
     6661 gtggttcgca tttcgccact tgcaaaacga attgccctcg aacataacat tgcttggcag
     6721 gaaatccaag gaaccggtca tcgtggtaaa atcatgaaga aggatgtttt ggccctgctt
     6781 cctgaaaata tcgaaaacga tagcatcaaa tctcctgctc agattgaaaa agtggaagaa
     6841 gtccctgata acgtaacacc atatggtgaa atcgagcgta ttccaatgac accaatgcgt
     6901 aaggttattg cccaacgcat ggttgaatct tacttgactg cgccaacctt caccctaaac
     6961 tatgaagttg atatgactga aatgttggct cttcgtaaga aggttcttga gccaatcatg
     7021 gaagcaactg ggaagaagac tactgtaaca gaccttcttt cacttgcagt tgttaagact
     7081 cttatgaaac acccatacat caacgcttca ttgacagaag atggcaagac tattatcact
     7141 cacaactatg tcaatcttgc catggcagtt gggatggata atggattgat gacacctgtt
     7201 gtttacaatg ctgagaagat gagtctttca gaactggttg tagcctttaa ggatgttatt
     7261 ggtcgtacct tggatggtaa attggctcca agtgagttac aaaattcaac attcacaatc
     7321 agtaatttgg gaatgtttgg tgttcagtcc tttggtccga ttattaacca acccaactca
     7381 gctatccttg gtgtcagttc gacaatcgag aagccagttg tcgtcaatgg tgaaattgtg
     7441 attcgcccaa tcatgagttt aggattaacc attgaccacc gtgtcgtaga tggtatggct
     7501 ggtgctaagt ttatgaagga cttgaaagag ttgattgaaa cgccaatctc aatgttaatt
     7561 taagagcaag agtattcatt tttaagatat agataaagga aggaagacat ggccttagaa
     7621 gtaattatgc caaaagccgg cgtggatatg acagaaggac aaatcgtcca atggaataaa
     7681 aaagtcggag aatttgtaaa agaaggagaa atccttttgg aaatcatgac tgataaagtc
     7741 agcatggaat tggaagccga agaagatggg tacttgattg ccattctcaa aggagatggt
     7801 gaaactgtcc ctgtaacgga agttatcggt taccttggtg aagaaaggga aaacatccca
     7861 acagctggag cagcatcgcc agaagctagc cctgtacctg tagcaagtac ctcaaacgat
     7921 gatggtaaga gcgatgatgc ttttgatatc gttgtgattg gtggaggtcc tgctggttat
     7981 gttgcagcca ttaaagctgc ccaactcggc ggtaaggttg cccttgttga gaaatctgaa
     8041 cttggtggaa cctgcttaaa ccgtggatgt attccaacca agacctacct tcataacgct
     8101 gaaattattg aaaatatcgg tcatgctgca aatcgtggta tcgtgattga aaatcctaac
     8161 ttcactgttg atatggaaaa acttttagaa actaaatcta aagttgttaa tactcttgtt
     8221 ggtggtgttg caggacttct tcgtagctac ggagttactg ttcataaagg aattggtaca
     8281 atcactaaag acaagaacgt cttggtaaat ggttctgaat tgcttgaaac caagaaaatc
     8341 attcttgctg gtggttcaaa agtcagcaag atcaacgtcc ctggtatgga atctccactt
     8401 gtcatgacta gtgatgacat tcttgaaatg aacgaagtgc cagaaagcct tgttatcatc
     8461 ggtggtggag ttgtcggtat cgaactcggt caggccttca tgacgtttgg ttcaaaagtg
     8521 actgttatcg aaatgatgga ccgtatagtt ccagctatgg atgcggaagt ttctaagaac
     8581 cttcgcttga tccttgagcg taaaggaatg accatcttga ctggtactaa actgcaagaa
     8641 atcattgagg aaaatggtca acttcgtatc aaggttgaag gaaaagacga tatcatagca
     8701 agcaaagctc ttctttcaat tggtcgtatg ccagaccttg aaggtattgg agaggttgag
     8761 tttgaattgg atcgtggttg tatcaaggtc aatgaataca tggaaacttc agttccaggc
     8821 atttatgcac caggtgacat caacggtact aagatgttgg ctcacgcagc tttccgcatg
     8881 ggtgaagttt ccgctgaaaa tgcccttaaa ggaaatcatg cagttgccaa attgaatttg
     8941 actcctgcag ccatctacac tctccctgaa gtagcagcag taggtttgac agaagaacaa
     9001 gcccgtgaga aatacgatgt tgccatcggt aagtttaact ttgctgctaa tggtcgtgct
     9061 attgcatctg acgcagctca aggtttcgta aaagttatcg ctgataagaa atacggagaa
     9121 atccttggtg tacacattat tggtcctgca gccgcagaat taataaacga agcatcaagc
     9181 atcatcgaaa tggaaatcac tgttgaggaa atgctgaaga ccatccacgg gcacccaact
     9241 tactctgaag tgatgtacga agcgtttgca gatgttctag gaatggccat ccattcacct
     9301 aagaaaaaat aaaacgaaga tagattagac tggaaggtta tttccagttt ctatcatata
     9361 aagtaaaggg atatcatgaa atacattatc aatcattcaa atgacactgc ttttaatatt
     9421 gccttggaag aatacgcctt taaacacctt ttggatgagg atcaaatctt cctactttgg
     9481 attaacaagc catctatcat tgttggtcgt caccagaaca ctatcgaaga aatcaaccgt
     9541 gattatgttc gcgaaaatgg tattgaggta gttcgccgta ttagtggtgg tggagctgtt
     9601 taccacgatt taaataacct caactacacg atcatctcca aagaagatga aaataaggca
     9661 tttgacttca agagcttctc aactccggtt atcaataccc tggctcaact tggggttaaa
     9721 gctgagttca caggtcgtaa tgaccttgag attgatggta agaagttctg tggtaatgcc
     9781 caagcctata tcaacggccg tatcatgcac cacggttgct tgctctttga cgttgatttg
     9841 tcagtcctcg caaatgccct taaggtttca aaagataaat ttgaatcaaa aggtgtgaaa
     9901 tccgtccgtg cccgtgtaac caatattatc aatgaattac caaaaaaaat cacagtcgaa
     9961 aaattccgtg atttactctt ggaatacatg aaaaaagagt acccagagat gactgaatac
    10021 gtcttttcag aagaagaatt ggccgaaatc aatcacatca aggatactaa gtttggaact
    10081 tgggactgga actatggtaa atcacctgaa tttaacgtcc gtcgtggaac caaattcact
    10141 agtggtaagg ttgaagtctt tgctaacgtt actgaatcaa aaatccaaga catcaagatt
    10201 tatggtgact tctttggtat tgaagacgtt gctgcagtag aagatgtcct tcgtggggtg
    10261 aaatacgaac gcgaagatgt tcttaaggcg ctaaaaacca ttgatatcac acgctacttc
    10321 gctggtatta gccgtgaaga aatcgctgaa gcggtagttg gataaatcaa aaagaagttg
    10381 gccaactaac caacttcttt ttgatattta tttacataat ttaaaatacg taaaattata
    10441 aactatctag agcattcttt tgttcatcat taacaatatg ggtatagagg tcagtgactt
    10501 gtgtgctggc atgtcctagt tggtgactga ctaaaacttg tgatttagtc gcatcataga
    10561 gcctagttgc tagcgtatgg cgcagtttat ggggtgttac acgcacttta aaatcctctg
    10621 agtatttagc aaccattttc tcaacgctag aagcatcgat acgattagga acacctctgt
    10681 agagagttaa aaaaagggct gtatctgttt tttccgtttt atagcgttga ttccgaatgg
    10741 ccagataatt ctctaaataa ggtttagcaa aagcagcgac attgactgag tcacgtttgc
    10801 caccttttcg agtaacatca ataaccatca tttttagatt gagatctctt agatctagat
    10861 taacagcttc agataagcga acaccagatg ccaagagaag ggcaataatg gctaaatctc
    10921 gttctttatt tttgttgaat gatgagagag ctcgatttga aagctgttgt gggtgctctt
    10981 gatcgatata agttagaaaa ccttctgttt catcacctag aaagagtttt tgcttgatat
    11041 tttcagctct ggcagcaagg gtttctttct ttttcttggt tgaaactttt ttcattacat
    11101 tacgatagaa ataaggttcc ccctgatcgt tttcaacctc ctcggttaga tacttgtaaa
    11161 gactagaaag tgctgataag gttcgattga tagttgtctg tgaaacacct tgttttgttg
    11221 tattagcatt cagcaaggga cgttcacgta gataaaggat aaaggattcc atgtctttct
    11281 tagacatatt ttccaaagct gataaaggaa tatcggatat tttatcagcg tttgaaatac
    11341 cagactccaa aacccagctg aaaaatcggt catattcctt aaggtattcg tacagggttg
    11401 taaaactgta gggtacagcc agcttagatt ggtagtattc cagaacatac cagggcatga
    11461 gttgttttag tttgtcgatt cgttccagta aaatctcacg tttcatgtat tttctccata
    11521 agtaagtata ctagtagtat agcatagact tgtatatttt tcaagaaaat tatagttttt
    11581 cagaaagatg ttataggagt ttttcaaagt gataaaaaga cttagaccta caaacaaaac
    11641 agaataagga aaatctcacc cactaaactt ctgttaatac tacataactg aagagctata
    11701 taaggagcta tgagatacaa aacacattgt ataatagatt gaaactagaa tagtacatct
    11761 ggacttctaa aacattgcta gaaattgatt tgattgtcct gattgtttta tcctatttct
    11821 atttcatttc actatataat tgctattgtt
//
LOCUS       DAVLYV010000057        11573 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLYV010000057 DAVLYV010000000
VERSION     DAVLYV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.057"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            35..1720
                     /locus_tag="VYO70_001511"
     CDS             35..1720
                     /locus_tag="VYO70_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat protein"
                     /protein_id="HEV1713724.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNDGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYLNESGSMATGWVKD
                     KGLWYYLNESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
     gene            complement(2212..2361)
                     /gene="rpmG"
                     /locus_tag="VYO70_001512"
     CDS             complement(2212..2361)
                     /gene="rpmG"
                     /locus_tag="VYO70_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1713725.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(2377..2559)
                     /gene="rpmF"
                     /locus_tag="VYO70_001513"
     CDS             complement(2377..2559)
                     /gene="rpmF"
                     /locus_tag="VYO70_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1713726.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            complement(2711..2911)
                     /locus_tag="VYO70_001514"
     CDS             complement(2711..2911)
                     /locus_tag="VYO70_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1713727.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            complement(2914..3921)
                     /locus_tag="VYO70_001515"
     CDS             complement(2914..3921)
                     /locus_tag="VYO70_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1713728.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALKRLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            4112..6142
                     /locus_tag="VYO70_001516"
     CDS             4112..6142
                     /locus_tag="VYO70_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1713729.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            6146..6430
                     /locus_tag="VYO70_001517"
     CDS             6146..6430
                     /locus_tag="VYO70_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1713730.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            6443..7789
                     /locus_tag="VYO70_001518"
     CDS             6443..7789
                     /locus_tag="VYO70_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1713731.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            7793..8650
                     /locus_tag="VYO70_001519"
     CDS             7793..8650
                     /locus_tag="VYO70_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1713732.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDENKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            8647..9579
                     /locus_tag="VYO70_001520"
     CDS             8647..9579
                     /locus_tag="VYO70_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1713733.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            9812..11515
                     /gene="ilvD"
                     /locus_tag="VYO70_001521"
     CDS             9812..11515
                     /gene="ilvD"
                     /locus_tag="VYO70_001521"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1713734.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETPIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
BASE COUNT         3629 a         1826 c         2387 g         3731 t
ORIGIN      
        1 atctattaga aaataaatat gtaaggaaat ttaaatgaaa aaaactacaa tattatcatt
       61 aactacagct gcggttattt tagcagcata tgtccctaat gaaccaatcc tagcagatac
      121 tcctagttcg gaagtaatca aagagactaa agttggaagt attattcaac aaaataatat
      181 caaatataag gttctaactg tagaaggtaa cataggaact gttcaagtgg gtaatggagt
      241 tactcctgta gagtttgaag ctggtcaaga tggaaaacca ttcacgattc ctacaaaaat
      301 cacagtaggt gataaagtat ttaccgttac tgaagtagct agtcaagctt ttagttatta
      361 tccagatgaa acaggtagaa ttgtctacta tcctagctct attactatcc catcaagcat
      421 aaaaaaaata caaaaaaaag gcttccatgg aagtaaagct aaaactatta tttttgacaa
      481 aggcagtcag ctggagaaaa ttgaagatag agcttttgat ttttctgaat tagaagagat
      541 tgaattgcct gcatctctag aatatattgg aacaagtgca ttttctttta gtcaaaaatt
      601 gaaaaagcta accttttcct caagttcaaa attagaatta atatcacatg aggcttttgc
      661 taatttatca aatttagaga aactaacatt accaaaatcg gttaaaacat taggaagtaa
      721 tctatttaga ctcactacta gcttaaaaca tgttgatgtt gaagaaggaa atgaatcgtt
      781 tgcctcagtt gatggtgttt tgttttcaaa agataaaact caattaattt attatccaag
      841 tcaaaaaaat gacgaaagtt ataaaacgcc taaggagaca aaagaacttg catcatattc
      901 gtttaataaa aattcttact tgaaaaaact cgaattgaat gatggtttag aaaaaatcgg
      961 tacttttgca tttgcagatg cgattaaact tgaagaaatt agcttaccaa atagtttaga
     1021 aactattgaa cgtttagcct tttacggtaa tttagaatta aaagaactta tattaccaga
     1081 taatgttaaa aattttggta aacacgttat gaacggttta ccaaaattaa aaagtttaac
     1141 aattggtaat aatatcaact cattgccgtc cttcttccta agtggcgtct tagattcatt
     1201 aaaggaaatt catattaaga ataaaagtac agagttttct gtgaaaaaag atacatttgc
     1261 aattcctgaa actgttaagt tctatgtaac atcagaacat ataaaagatg ttcttaaatc
     1321 aaatttatct actagtaatg atatcattgt tgaaaaagta gataatataa aacaagaaac
     1381 tgatgtagct aaacctaaaa agaattctaa tcagggagta gttggttggg ttaaagacaa
     1441 aggtttatgg tattacttaa acgaatcagg ttcaatggct actggttggg ttaaagacaa
     1501 aggcttatgg tattacttaa acgaatcagg ttcaatggct actggttggg ttaaagacaa
     1561 aggcttatgg tattacttaa atgaatcagg ttcaatggct actggttggt ttacagtttc
     1621 tggtaaatgg tactatacct ataattcagg agatttatta gtaaacacga ctacacccga
     1681 tggctatcga gtcaatgcta acggtgagtg ggtaggatag gtatccatta gctaaatcta
     1741 atcattctga actaaaaaat cttcttacat atgtaggaag attttttgga ttctcaaaac
     1801 tagtttaaaa gactacgcta tttaaaccaa ttttaagaat ggatatataa ggatattcag
     1861 aaatctagtt ggtcaacttt tgtattttga atatacctta gatggtgacg atgtcttggt
     1921 atatctggta ctcacttaca tattctaact tattttaact aagatacaac tgtagttggt
     1981 gaatttgcta caaatagtgt agaggcgtta tttgaaaaat atacaaaaaa ggaaccccaa
     2041 cttacatttg agtttctctt acttttaatc gtccgaatcc gtattcttat ttcaaaaaag
     2101 gtagtgaaaa atgtagtcat tcgaatgatt tcccttgcaa tatagtgaaa ttaacaaaaa
     2161 gaaaaaacgc tgatcaagcg tttttcttct gtattgattc gtattgaatg cttacttaac
     2221 ttctgtaaac acaacgtgtt tgcgaagttt tggtgagtat ttcttcaatt gaaggcggtc
     2281 tggagtgtta cgtttgtttt tagaagtaag gtacaagcgt tcaccagatt ctttgtgttc
     2341 aagtgtaata tttacgcgca tggtatctcc cttctattat tcagctgatg cagctttagt
     2401 gattttacgt cctttgtagt atcctttaag tgatacacgg tgagaacgtg agtaatctcc
     2461 agtagtttcg tcaaagttta cagatggagc tgttactttg tagtgtgtac gacgtttgtt
     2521 tttcttcgct tttgaagtgc gacgtgcagg tactgccatt ttgatttctc ctttaggtat
     2581 ttatattcga ttcaatctac tgattttcat caaccatact aggataacac attttttttg
     2641 taaagtaaag ttacttgaca aaaaaagatg aaaaaattct cctatccgac acggcagaca
     2701 ggagaaggtg ttaaatatca atctcaaatg gttcgtcaat ggtttctgat acgtattttc
     2761 cgtctttctt ccgttgcttg acacactctg tgaggagata ttcgatttgc ccattgactg
     2821 aacgaaagtc gtcttctgcc catgatgcga gtgcagcgta taactttgtt gagagtcgaa
     2881 gggggatctg ctttttttta gcttcagcca tctttagtaa agacttcctg tgttgacaat
     2941 tggttgtgca tcatgattgc cacaaagaac gacaaggaga tttgaaacca tggcagcttt
     3001 tcgttcttcg tcaagttcta ccaattcccc ttcattgagc cgttttagtg ccatttcaac
     3061 cattcctaca gcaccatcta caatcatctt ccgtgcatca ataatggcag atgcttgttg
     3121 gcgttgaagc ataacggcag caatttctgg agcataagct aggtaagtga tacgtgcttc
     3181 aaggatttcc aagccagcat cctcaacacg actttggatt tcttcacgaa tacggttagc
     3241 aacaatttcg ctagagccac ggagactacc ttcatctgct tgcccgtcac ccgtagtatc
     3301 cacattagga gacacatcgt aaggatagat gcggacaata ttacggaggg cgctatcaca
     3361 ttgcagtgaa agatattctt tgtagttatc aacgttgaag actgccttag cggtatcgac
     3421 aactctccaa gttaccgcga taccgatttc tacagggttt cctaagcaat cattgatttt
     3481 ttgacgagaa ttgctcaagg tcatgacttt gagggaaatg tgtttcttgc caatttcaag
     3541 atttacatca ttgtcatttg atgatttagc tcctaaaaaa ggagattttg tgctaacatc
     3601 accactttgt ccaagtcgag tgtggtttgc agggttgact gctacgctga agggattgac
     3661 aaagtaaaaa ccaggttctt tgatggtacc tgtataatta ccaaagagtg tcagaaccag
     3721 agcttcctga ggtttgacaa cttttaaacc agcatgagct agacctgcaa ttacgattag
     3781 taaaggtccg ataataattc caaaaatgtt ttccgaacca acccccatta taaatataaa
     3841 gataccaagt acgattgtca actcaatgag aatcaatgcg aatacaccat ttggttttga
     3901 attgattagt ttttcagtca taagaatgac ctcccattat ttgatatcta aatgatatca
     3961 ctttgttttt aaaagtcaat agaaagttga aaataatcac taaaaagttt cagcccaata
     4021 atttctttta aaaggtgaga ttatattgtg ctcaaatact cttttattct tataattttt
     4081 acactataat tgtagttaga aaggaagaga aatgtttgat tatagagcac tagttatttt
     4141 gatgactttg atggatcatt gtgagctatc attatatgaa ttatctgtta aggtgagctt
     4201 acctataaag gaagtcaaag aaggaataga ttatttagtg ccttatctag ctaataaagg
     4261 gatagtgctg gataaaaagc aaggtcgcta tagtttatca aatcgtacga agcagtcttt
     4321 gacagatatt attaagtcgg atgaattggt tttaccaaag tcgactcgct tagctttaat
     4381 ctatctttac actttttgcc gattagattt tatatcgaat agtcattacc aagacttttt
     4441 gaaagtaagt aagaatacaa ccttatctga tattcaatca ttaagaaaga ttatgttaga
     4501 taatgatttg gagctagggt acagtagagc aaaaggttat actttacacg gttcagagtg
     4561 gaataagcac cgcttagctt ttcaaatggt tagtgagttg ctggaatcct ccatagggat
     4621 ttggggtttg gattatgttt tatccagttg ggggtattca ttaacttatg atttgattga
     4681 tcaggtagtt aaagattatt atgagaagct acagctagta cctattgtta atcaattaaa
     4741 agtttacctt ttcggtttag tattcattct gtgtaggtat caaagggatg ttgaaagagt
     4801 atgtctttca gagacattag tatctcctgt tattcaagat ataaccacta ttttattgga
     4861 tacagtggtt gatttgggaa ttatagatac ggtgttttct gaggatgatt atcgctatat
     4921 caccgtttta ttatcaagtt gctttgaagg tgaagtagat gttgctccgg tttactttaa
     4981 tcaattaaca gaggctatta taagtagaat ggaagatatt tctttgttac attttaaaca
     5041 aagggaagga ttgagagaaa atcttcgccg ccaccttata ccggcttact atagattaaa
     5101 gttcggccta cctagttcaa atgaatatgt attgcatgtt aaagaacatt atcctgattt
     5161 atttgaatta gtcaaagatt ctttgatgcc cttgatggac gctattgaca atcccatacc
     5221 tgatagtgaa acagcatatt ttgttatcca tttcggaggt tatttaaaga aagcagatac
     5281 tttgcctcaa aaatggtata aagcagcaat catttgtcct aatggtgtta gttcttcatt
     5341 gatgttaaaa gagaatctat tagcattatt tcctcaaatt gagtttatag gaacctcaaa
     5401 gattgatgat ttacaggtga aagctagtag tgactatgat atggtttttt ctaccataaa
     5461 ggtggagaca gaaaagccaa attatctagt ttcggttatg atgactgaag agcaagcaat
     5521 acaactagta gaactagtgt taaaagattt tccgaattta gagtatggag attttgagat
     5581 tgaacaaatc ctgaatattg tcaaaagata tggaattatt acacaagaat tggaattaag
     5641 attggcgtta aagaattatc tttaccaaaa aaatgataga aaggaaattg taccagtgct
     5701 agaacaactt attaccaaag aaacctatca ggttagttcg caaaaattag gatggaagga
     5761 ggcgattcgt ttagcagcta aaccgctttt ggatcaagat aagataactg agaactaccc
     5821 tgaggcaatg attcaaaaag tagaagagtt tggacctttt attaatttag ggaagggagt
     5881 agcaattcct cacgctcggc cagatgaagg tgtgaatgaa ataggaatgt caatgttagt
     5941 tttggaagaa ccgatttatt tattggataa tccagaacaa gaggtaagat tgttgatttg
     6001 tattgcagcc attgataatg agagtcattt aaaggctttg tcacatttaa caacaatttt
     6061 aagagataaa aatcatgttc aaactttaat atcatcaaaa aactataatg atattaaaat
     6121 gataattaaa caggaggatt agataatgtt aaaaattggt acagcttgtg gttcaggatt
     6181 aggttcaagt tttatggtac agatgaatat tgaatctgta ttgagtgatt tgaatgtttc
     6241 ggatgtagaa gttgaacatt atgatttagg tggagcagat ccaaatgcag ctgatatttg
     6301 gattgttggt cgtgatctag ctgattcagc tagtcatctt ggagatgttc gtatcttaaa
     6361 tagtattatt gatatggatg aactacgaga attaattact aaactttgtg aagaaaaagg
     6421 acttatatag gaggctagcg tcatgatgaa gttcatattg gatattgtta gtacaccagc
     6481 tattttagta gctttaattg caattttagg attagttctt cagaagaaga aattacctga
     6541 tattattaaa ggtggaatta agacctttgt tggtttctta gttgtatctg gtggtgcagg
     6601 aattgtacaa aattctttaa atccatttgg taccatgttt gagcatgctt ttcatttatc
     6661 tggcgttgtg ccgaataatg aagcaattgt agctgtagct ttaacaacat atggctcagc
     6721 tactgcaatg attatgtttg caggaatggt gttcaatatc ttaatcgctc gttttactcg
     6781 atttaaatat atttttttaa cagggcacca cactctatat atggcatgta tgattgcggt
     6841 cattttatca gttgctggct ttactagctt gcctctcatc ttactaggag gattagcact
     6901 cggtattatt atgagtattt ccccagcatt tgtgcaaaaa tatatggttc aattaactgg
     6961 aaatgacaag gtggctttag gtcatttcag ttctttggga tattggttga gtggttttac
     7021 tggtagcctt atcggtgaca aatcaaaatc aacagaggac attaaatttc caaagagttt
     7081 agctttttta cgtgatagta ctgttagtat cactttatct atggcagtta tttacattat
     7141 tgtagctatc tttgcagggt cagaatatat agaaaaagaa atcagtagtg gtacaagtgg
     7201 tctagtttat gctttacaat tagcaggtca atttgcagca ggggtatttg ttattttagc
     7261 aggtgttcgc cttattttgg gcgaaattgt tccagccttt aaaggtattt cagagcgtct
     7321 tgtacctaat tcaaaacctg ctttggattg tccgattgtt tatacttatg cacctaatgc
     7381 agttctaatt ggatttatct ctagttttat tggtggttta gtaagtatgg caattatgat
     7441 tgcttcagga acggttgtta tcttaccagg cgttgtgcct catttcttct gtggagcgac
     7501 tgcaggtgtt attgggaatg catctggtgg tgttcgtgga gccactattg gagcattttt
     7561 acaaggtatt ttaatcagtt ttctcccagt ctttttaatg ccagttttgg gaggactcgg
     7621 tttccaagga tcaactttct cagatgcaga ttttggtcta tcaggaatta ttttaggaat
     7681 gttaaatcaa tttggctcac aagcaggcat tgtgattggt cttgttctta ttttagcagt
     7741 tatgtttgga gtatccttta ttaaaaagcc atctgcaaag gaggaataag ggatgatttt
     7801 aagtaaaaat agagaagatg agttaagaaa atttgcgact aacatccgat taaatactct
     7861 tagaacattg aatcatcttg gattcggaca ttacggagga agtctgtcta ttgtagaagt
     7921 tttagcagtg ctttatggtg aaataatgcc aatgactcca gaaatatttg cagcacgaga
     7981 tagagattat ttcatattat caaaaggtca tggaggacca gctctataca gtacactcta
     8041 tttgaatggt ttctttgaca aagaattctt atattcttta aatacaaatg gaaccaaatt
     8101 accgtctcat cctgatagaa atctaacgcc aggcatagat atgacaacgg gctctttagg
     8161 acaaggaatt agtgttgcaa ctggacttgc atatggtcag agaataagaa agagtccctt
     8221 ttatacttat gctattgttg gagatggtga gttaaatgaa ggacaatgtt gggaggctat
     8281 acagtttgct tctcatcaac agttatccaa cttaattgta tttgttgatg agaacaaaaa
     8341 acaattagat ggttttacaa aggatatttg taatccaggt gatttcgtag aaaaattttc
     8401 agcatttgga tttgaatcca ttagggtcaa gggttcagat attagagaaa tttatgaagg
     8461 gattgtccaa ttaaaacagt caaataattc atcacctaag tgcattgtat tagatactat
     8521 taaaggtcaa ggggttcaag agctggaaga aatgaaatcc aatcatcatc ttcgccctac
     8581 tgtagaggag aaacaaatgt taacttcagt tgtagaaaga ttaagtcagg aattggagga
     8641 aacagaatga tgagaagtac gaaagaatta cggcatgtat atagagactt ccttttagag
     8701 gctaatcaaa gtgattctga tatagtagtc ttagaagccg atttgtcaag ttcgatggct
     8761 actcataatc ttgaaaagga ctttggagac cgttatgtga atgttgggat tatggaagca
     8821 gaaatggtcg gccttgcagc aggcttgtct attcaggggt ttagacctta tcttcataca
     8881 tttggtcctt ttgcttcacg aagagtattt gatcaattat ttatttctct tggatacgca
     8941 caattggatg ccactgtgat tggatcagat gcaggagtaa ctgcagaaat gaatggtggg
     9001 acacatatgc catttgaaga aattggattg ttacgtttaa ttcctaaatc gatcattttt
     9061 gaagcaactg atgatattca atttcgtgaa atattgaacc agacattaga cttaaaagga
     9121 ctaaaatata ttcgaacaat tagaaaagct ccagttcctg tgtatcaagg tggagaagat
     9181 ttttctaaag gctacattga gttaagacac ggggaagatc ttgtaatcgt tgcttctggt
     9241 ataatggttg ctccaagtat tcgagttgca gatgaactgt ctaaattagg ttactcagta
     9301 ggtgtgatag atttatttag aatcaaaccg ataccagaac agataaaaac aatgttaagt
     9361 ggaaaaacta tatttactgt agaaaaccac aatcagatag gtggaattgg cagtgcttta
     9421 tgtgaattat tctctgatga tggaataaca aaaattcatc ggatgggggt tcaagaaaga
     9481 ttcggtcaag tagggaaaat ggattatctt cttaatgagt atggtttgag tgaatcgaat
     9541 ataaaagaga aggttctaga aatttataat gcaagataga tgttaaatac acttatctga
     9601 aagaatttct gtaaaaatta cattacgtta tttaaagaaa gcggattaaa aagcatattc
     9661 tggtcatcag aaagtgcact aatccgcttt gttgttttta attttaaata tttcacatgc
     9721 gtatagaatt ttctgaaaat tcaagaattt tattctgttc attgccttta aattgtgcta
     9781 taatagtaaa aactgaaatg ggagggataa gatgactgaa ttagataaac gtcaccgcag
     9841 tagcatctat gacagcatgg ttaaatcacc taaccgtgct atgcttcgtg cgactggtat
     9901 gacagataag gactttgaaa caccgattgt gggagtgatt tcgacttggg cggaaaatac
     9961 accatgtaac attcacttgc atgatttcgg gaaactggct aaagaaggtg tcaaatctgc
    10021 aggcgcttgg cctgtacagt ttggaaccat taccgtagcg gacgggatcg ctatgggaac
    10081 acctggtatg cgtttctctc taacatctcg tgacatcatc gcggactcca tcgaggcggc
    10141 tatgagtggt cacaacgtgg atgccttcgt cgctatcggt ggctgtgaca agaacatgcc
    10201 tggatctatg attgctattg ctaatatgga tatcccagcc attttcgcct atggtggaac
    10261 tattgcaccg ggaaatcttg atggtaaaga tatcgacttg gtttctgtct ttgaaggtat
    10321 cggaaaatgg aaccacggtg atatgacagc tgaggacgtg aaacgtcttg aatgtaatgc
    10381 ctgccctggc cctggtggtt gtggtggtat gtatactgct aataccatgg caactgctat
    10441 cgaagttcta gggatgagtt tgccagggtc atcctctcac ccagctgaat cagctgataa
    10501 gaaagaagat atcgaagcag caggacgtgc tgttgttaag atgttggaac ttggtctcaa
    10561 accatcagat atcttgactc gtgaagcctt tgaagatgct atcactgtaa cgatggctct
    10621 cggtggttct acaaacgcca ctcttcactt gctcgccatt gcccatgccg caaatgttga
    10681 cttgtcactt gaggacttca atacgattca agaacgtgtg cctcacttgg ccgacttgaa
    10741 accatctggt cagtatgtct tccaagacct ctacgaagtc ggtggtgtcc ctgcggttat
    10801 gaagtatttg ttggcaaatg gtttccttca cggagatcgc atcacatgta ctggtaagac
    10861 tgtagctgaa aacttggctg actttgcaga cttgactcca ggccaaaaag ttatcatgcc
    10921 acttgaaaat ccaaaacgtg cggatggtcc gcttatcatc ttgaacggga accttgctcc
    10981 tgacggtgca gttgccaagg tatcaggtgt taaagtgcgt cgtcacgttg ggccagctaa
    11041 ggtctttgac tcagaagaag atgcgattca ggccgttctg acagatgaaa tcgttgatgg
    11101 cgatgtagtc gttgttcgtt ttgttggacc taaaggtggt cctggtatgc ctgagatgct
    11161 gtcactttct tcaatgattg ttggtaaagg tcagggagat aaggtggccc tcttgacgga
    11221 cggacgtttc tctggtggta cttatggtct ggttgttgga catatcgctc ctgaagctca
    11281 ggatggtgga ccaattgcct atctccgtac cggcgatatc gttacggttg accaagatac
    11341 caaagaaatt tccatggccg tatccgaaga agaacttgaa aaacgcaagg cagaaacaac
    11401 cttgccacca ctttacagcc gtggtgtcct cggtaaatat gcccacatcg tatcatctgc
    11461 ttcacgcgga gccgtgacag acttctggaa tatggacaag tcaggtaaaa aataaactca
    11521 tactcgaaaa tctcttcaaa tcacgtcaac gtcgccttgc cgtaggtatg gtt
//
LOCUS       DAVLYV010000058        11223 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLYV010000058 DAVLYV010000000
VERSION     DAVLYV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11223
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.058"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..769
                     /locus_tag="VYO70_001522"
                     /pseudo
     CDS             1..769
                     /locus_tag="VYO70_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(885..1688)
                     /locus_tag="VYO70_001523"
     CDS             complement(885..1688)
                     /locus_tag="VYO70_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1713735.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYENATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1965..2720)
                     /locus_tag="VYO70_001524"
     CDS             complement(1965..2720)
                     /locus_tag="VYO70_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1713736.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2790..5441)
                     /locus_tag="VYO70_001525"
     CDS             complement(2790..5441)
                     /locus_tag="VYO70_001525"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1713737.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDARFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTINPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(5434..6012)
                     /locus_tag="VYO70_001526"
     CDS             complement(5434..6012)
                     /locus_tag="VYO70_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1713738.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(6012..6572)
                     /locus_tag="VYO70_001527"
     CDS             complement(6012..6572)
                     /locus_tag="VYO70_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713739.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGFP
                     PVKTLEDEIYYLEHILPERNQKENLPAGYGIVVKGTDKIVGSVDFNHRHEDDVLEIGY
                     TLHPDYWGRGYVPEAARALIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDVQGNRCDSLIYGLLKSEWEVI"
     gene            complement(6575..7138)
                     /locus_tag="VYO70_001528"
                     /pseudo
     CDS             complement(6575..7138)
                     /locus_tag="VYO70_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            complement(7422..7817)
                     /locus_tag="VYO70_001529"
     CDS             complement(7422..7817)
                     /locus_tag="VYO70_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1713740.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7913..8167)
                     /locus_tag="VYO70_001530"
     CDS             complement(7913..8167)
                     /locus_tag="VYO70_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1713741.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKAGKGFHDLPEGLFGERNY"
     gene            complement(8177..9511)
                     /locus_tag="VYO70_001531"
     CDS             complement(8177..9511)
                     /locus_tag="VYO70_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1713742.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEVFEAFAIEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9511..9744)
                     /locus_tag="VYO70_001532"
     CDS             complement(9511..9744)
                     /locus_tag="VYO70_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1713743.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            complement(10159..10341)
                     /locus_tag="VYO70_001533"
     CDS             complement(10159..10341)
                     /locus_tag="VYO70_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713744.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(10343..11122)
                     /locus_tag="VYO70_001534"
     CDS             complement(10343..11122)
                     /locus_tag="VYO70_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713745.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKTLWYLILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
BASE COUNT         3305 a         2468 c         2079 g         3371 t
ORIGIN      
        1 gacctactac tatcacttga aacagctaga caaaacagat aaggaccaag agcttaaagc
       61 tgaaattcaa tccattttta tcgaacacaa gggaaattat ggttatcgtc ggatttattt
      121 agaactaaga aatcgtggtt atctggtaaa tcataaaaga gttcaaggct tgatgaaagt
      181 actcaattta caagctaaaa tgcgacagaa acgaaaataa aggagacgtt ggtaagaagg
      241 cagagaatct cattcaaggc caatttgaag gatctaaaac aatggaaaag tgctacacag
      301 atgtgacaga atttgccatt ccagcaagta ctcaaaagct ttacttatca ccagttttag
      361 atggctttaa cagcgaaatt atcgcctata atctttcaac ttcacccaac ttagaacaag
      421 tacaaacaat gttggaacag gcattcacag agaagcacta cgagaatacg attctccata
      481 gtgaccaagg ctggcaatac cgacacgatt cttatcatcg gttcctaaga gtaagggaat
      541 tcaagcatct atgtcacgca agggaaacag ccaagacaac ggtatgatgg agtccttctt
      601 tgggattctg aaatcggaaa tgttttatgg ctatgagaaa acatttaaat cacttaatca
      661 attggaacaa gctattgtag actatattga ttactacaac aataaacgaa ttaaggtaaa
      721 actaaaagga cttagccttg tgcaatacag aactaaatcc ttcggataaa ttatttgtct
      781 aactttttgg ggtcagtaca cctaaaactt tgatgatata cgttttcttg tgagaatatt
      841 tacttcattt ttgcctaaaa ttcaatgttt actcagtatt tggattatga aaaatcgagg
      901 tctaaatcta gatacatttt ttctgaagac aaatcatttt gaccaccgag caagagattt
      961 tcaaaaaaag ctgttaaaaa ctcagaacgt cgctgtaaaa tctttgcatt atctaatacc
     1021 aaggcatcac gaaaatattt ggaatgttgc tgaaatggtg tattatcaat atcaaaacca
     1081 aactcacgaa gatactgaat caaaaagacc gttactgtcc gagtgtttcc ttcgcgaaat
     1141 ggatgaatct gccagattcc tgaaataaaa tgctggattt gtttaaccac atccgcctga
     1201 gttagtgtcg catatgcaac ttgtttttcc tgattaaaat cataatctaa ggtcatttga
     1261 atcatggagt aatcagagta cacaacactt tcaccattca aaacaggttc attctttgtg
     1321 atattggtct gacgaaattg ccccaccgga atagagggtt caaatatatc ttgaaacaac
     1381 tccttatgaa tagcaagtaa ggtcgcagga ctaaagctaa agcctcttcg agacaatagt
     1441 tctacaatac gtagagaaac caagtctgcc tcttccgtac ttgcatcaat agtatggtga
     1501 taagccgttg cattctcata aacctgctca taagtcagtt ctccccggga ctgtttctca
     1561 gccaaagatt ccatatacgc tgatggcact agattgtcaa ctttctgcag accaaaacct
     1621 atccgccata aatcacgctt cgcttcataa gacaagtttg gattgtcaat gttgtaagtt
     1681 gattgcatag aaatatcctt tcaattgttt ttcttcatta ccagtaaatc acgaaattgg
     1741 ttggaaactg gcttaaacta taaaaagtcc tcacaatagg ttctatgata tatatatata
     1801 cttcatgtaa ataataaaag agttataatc agcactactg ataaattcaa taccgatttc
     1861 gccatcttaa aacttagtca aacgaatacc gttgatttcc agttcattaa gtcgttgacc
     1921 tgtcaagact tctcgttcct tcaactggaa aagttaaggg ggaactaata caaatcctct
     1981 acgcttctat attcaatgat ttcatttttg ataatattct ttttaaactc aaaatgtttt
     2041 aaaggattat cgattaacac taactttgga atatcatcta gatttgtgac caaggataaa
     2101 ataaaatccg attgaaactc cttgactttt tcaaattctt ttgcagttaa acgcacacgc
     2161 cccttagatt ttcggatacc ttttacttcg gcaaggtaag aatactcttg gacatctacc
     2221 tgaaagtcat atccatcacc ataaagacgg gcatcggtta actgtccacc ttggaatttt
     2281 tcttctttat caaaatgaag gataaaataa ttctcagcct ccattcccgt ttcctgtaga
     2341 cgtttgaatt tagttcgaac tattggtttt tcaacgatgg ttatgcctgt tttcttccct
     2401 tcactggcaa gtaacatttt aactatttca gcataactat gaacatcttc atttccaaac
     2461 atcatatcaa ttaaatcttt acgaaaacga taaacttcag ctttctgcca ccaacctttt
     2521 cgattattgt caaaataagg atcaaataaa tccattctat tttttacaac tccagttgtt
     2581 tcagcaattc ccagagaaac cacatagcga taaaactccg acttgctaga gcattgaaat
     2641 tcttttataa aaagcttgtc aaacttggca agaccgtagc caattaaatt taacaattca
     2701 taatgagggt gtttggacat cactttctcc atcaatagat attgatttca ttataccaaa
     2761 aaacacggct catcaccgtg tttctgtgtt tatttcacca acttcttcat ctcatcaata
     2821 cgtgctacgg tcgcatcata cttagcttgg taatctgctt gcttgtcgcg ttctttttgg
     2881 acaacttctg gtttagcgtt ggcgacgaag cgttcgttgc tgagtttttt gccaaccatg
     2941 tcgagttctt tctgccattt ggcaagttct ttttccagac gtgccaattc ttcttcgaca
     3001 ttcaagaggt ctaccagtgg caagaagatt tcggctcctg tgatgacgct tgacatagcc
     3061 agttcaggtg cagggatggt tgatgcgatt tccaagtgtt caggatttgt gaaacgtttg
     3121 atgtagttga cattgctgtt aaagaaggct tccaagtcgc tatcgcttgt cttaacaagg
     3181 atagtgatag gcttgcttgg agcaacgttt acttccgcac gcgcattacg aacggcacgg
     3241 atcaagtctt taaggctttc cacaccagtg tgagcagcaa ggtcttcaaa ggctgggttg
     3301 atagttgggt attctgctgt aacgatagat ccttctgaga tttgtccaaa gatttcctct
     3361 gtcacaaatg gcatgattgg gtgaaggaga cgaaggatct tgtccaaagt gtatagtaga
     3421 acagaacgtg tgatgacttt ctcttcttcg ttatcgctat aaaggacttc cttggtcaac
     3481 tcaacatacc agttggcaaa ttcttcccag atgaagttgt aaaggatatg accagccaca
     3541 ccaaactcga acttatcaaa gttttcagta acttttgcaa tggtttcgtt gagattgtgg
     3601 agaatccagc ggtccgtcac attaccagcc tcacctgttg caacttttgt gacattgtca
     3661 tgcgccacat ccagcgtcaa accttcattg ttcatgagga tatagcgaga aatgttccaa
     3721 attttgttaa taaagttcca tgaagcatcc attttctcgt aagagaaacg agcgtcttga
     3781 ccaggagctg agccgtttga caagaaccaa cgaagactat ctgtaccata tttatcaata
     3841 acatccattg ggtcaatgcc atttccaaga gatttagaca tcttacgtcc ttcttcatca
     3901 cgaattagac catggataag agcattttta aatgggctct ttcctgtaaa ttcaattcct
     3961 tggaaaatca tgcgtgatac ccagaaagga ataatatcat agcctgttac gagtgttgaa
     4021 tttggatagt agcgtttaaa gtcagtagtt tcggtattag gccagcccat ggttgagaag
     4081 ggccagaggg cagagctgaa ccaagtgtca aggacatctt cgtcctgttt ccagccatcg
     4141 cccgctgggg cttcctcgcc gacatagatt tcgcctgctt cattatacca agcagggatt
     4201 tggtgacccc accaaagctg acgagagata acccagtcgt ggacattttc catccattga
     4261 aggaaggtat cgttgaaacg aggtgggtag aattcgacct tgtcctctgt gtcttggttg
     4321 gcaatggcgt tcttagccaa ttggtccatc ttgacgaacc attgagtaga caagcgtggc
     4381 tcaactacga cacctgtacg ttctgagtga ccgactgaat ggacacgttt ttcgattttg
     4441 acaagggcac cgatttcttc caacttagca acgactgcct tacgagcttc aaaacgatcc
     4501 atgcctgaaa attcaaaggc aagctcattc atagttccgt cgtcgttcat gacgttgact
     4561 tgtggcaagt tatgacgttg gccaaccaag aagtcatttg gatcgtgggc aggtgtgatt
     4621 ttcacgacac cagtaccaaa ctcaggatct gcgtgctcat ctccaacgat tgggatgagt
     4681 ttattagcga ttggaaggat gacgttttta ccaatcaagt ccttgtagcg cgggtcttct
     4741 ggattaaccg caaccgcaac gtccccaaac atagtctcag gacgagttgt agcaacttca
     4801 agggcgcgtg aaccatcttc cagcatgtag ttcatgtggt agaaggcacc ttcgacatcc
     4861 ttgtgaatca cctcaatatc agaaagggct gtgcgagctg ctgggtccca gttgatgata
     4921 aactcaccac ggtagatcca gcctttcttg taaaggtcca caaagacctt acgaacagct
     4981 tttgacaaac cttcgtcaag agtgaaacgc tcacgagagt agtctacaga gagccccatc
     5041 ttgccccatt gttccttgat agtagtggca tattcgtctt tccattccca gactttcgtc
     5101 aagaaagact cacgaccaag gtcatagcgg gaaatgccct caccacgcaa gcgctcctct
     5161 accttagcct gagtggcaat ccctgcgtgg tccatcccag gaagccaaag ggtatcaaaa
     5221 ccttgcatgc gtttttgacg gatgataata tcttgcaaag ttgtatccca agcgtgacca
     5281 aggtgaagtt tacctgtaac gtttggtggt ggaataacga ttgaataagg cttagccttt
     5341 tgatcgcctg aaggcttgaa aacatcagca tcaagccatt tttggtaacg accagcctca
     5401 acctcggctg gattgtattt agatgaaagt tctttagaca tgtgtgtgtc ctttctctat
     5461 tttgtttatt ttattttgaa tctgcttagc agcttcttct gcagacaaat tcgtattatt
     5521 tattttaaag tagtggtgca actcattcgg ttgatattgg gaatttaatt gaagtgtttc
     5581 agcggtctct aaaatttctc tttcagatac ctcaatatgt cgttttaagg gtttgtgctt
     5641 taatcgattc tccgttcgat ttcgacgtat gcactcttca agacttgttt ccaactcaac
     5701 aaaaagaatc tcttgatgat aatcatccaa caaattctga atttgcttta aatatatcag
     5761 atgatactga tttgaaaaat caattacatc tgttaaaatt actgatcgct gatttcttgc
     5821 acttgctcca aggaatgaaa aagtgattcc acgaacaaat tcccacattt cctctgtata
     5881 gtcctgatag atttctagtg caaaatcaat ggcttgatgg ttataaaata gggtagcatc
     5941 cgtcagtcga gataattctt gaccaatggt catttttcct gatgctggag caccaatgat
     6001 gaaaagatgc atcaaatcac ctcccactcg ctcttcagca agccatatat cagactgtca
     6061 cagcggtttc cttgaacatc ctttcggtct cttatgcgag cttcgagggt aaagccaagc
     6121 ttttccgcga ctcgtttact ttgaaggtta tatccaaagc aagttagttc aatcttgtga
     6181 agacccaaat ctttaaaggc taagtcaatc aaggcacgcg cagcttctgg cacataacct
     6241 cgcccccaat agtctgggtg taaggtatag ccgatttcca gcacatcatc ttcatggcga
     6301 tggttgaaat cgacagagcc aacgatctta tccgttcctt tgacgacaat cccgtagcct
     6361 gctgggagat tttccttttg attacgctcc ggaagaatgt gctccagata ataaatctca
     6421 tcttccaagg tcttgactgg aggaaaacct gctggatagg cgacctctgg caaactagca
     6481 tagtcaaaga tatcctcagc atccgccaca gtacggactc gtaaaacgag acgttctgtt
     6541 tcgattttat ctggcagctc agttcttgcc atccttcttc ctcgcttttt ttgatgaaac
     6601 tgcccttcat atctacacgc ttgtccagat agcgataaac gcgctgatat acatctccca
     6661 tgaaataggt tggggcaaac agttgatttt taaaatgtcc cttttcatcc aggagttctg
     6721 gggcaacaag tcgctcaaga atcttggcaa agatgtggca aataccgtct tcctcaacaa
     6781 tcctatctac ccgacaatct aaaacaagtg gacaggcgtc taaaatagga gtctgagttc
     6841 gttcagaaat ttcataatgc actcccaaac gttccaattt ctcctgatga ctgataaaac
     6901 cagcctgctc catcgcaagc atagaagttt catcagaaat attcacagta aatttttgat
     6961 actgtttgat ctgctctgcg gcattctctc tcgcaacgac tccaattaca acccaatctc
     7021 ctagactata cgaggaacta caggtcgtaa tgttatagcc aaaattctga tcttgatatc
     7081 ctaaaataaa aacaggaaaa ctataatata gtttattagt tttaaatgat tgttttataa
     7141 caacctcctt tgactaagac acaaaagaaa agccctgcca tctacatgac agggacgaat
     7201 gtatttatcc gcggtaccac ccaatttcgg gcagttgccc gcaactctcg tctttaaagc
     7261 aaaaagacac ttttatttca atttttcatc cattagcaac cagttttgac acatttgtgg
     7321 acttctcagc accgccactt tctgtaaaat gtgggattca aaacacctct aatggctcta
     7381 ttgtagcaag tttttctcgg aaatgcaagg ctcaagaaaa attacttgaa cttgatgcca
     7441 ttttctttca atttcttgat ggtagctggg ccgattcctt tcaaggcaag gagttctttt
     7501 tcagtccagt ttttgaaatc tgaagcagac ttgattcctt catcatagaa agtcttggca
     7561 cgatcaagtg gaagtccacc caagtttgct gcaaaatctt ctacagaatt agctactttt
     7621 tgagcttgct ctttggtagc ttctactgct tgttcaactt ttttagaaac tgccttacca
     7681 gctgcgcttg ctgactgttc tgcacgcttc acgacttttt ttgtctcatc tacaacctta
     7741 gtcacagtac ttgaaaaggc acctgcgcga cggaggccat ttcgtaattg ttttttacga
     7801 ttgagtttct ttgacatgat aaaatccttt caataaaaaa tccctgctct gacaaggatt
     7861 taacataaac attctatcaa gattttagtg aaaaatcaag aatctttctc gtttaataat
     7921 ttcgctcacc aaacaagcct tctggcaaat catggaatcc cttgcctgct ttgaagtttt
     7981 caaacttacc aagcaagaac tgataagcat ccaagcgact ctcgtagcga atcatggcat
     8041 ctttggcacg ctcgtcctgg tcttcttcgt aaactttttg gctttccttg tgcgccatgt
     8101 cgttaatcat gatttgggta ttgacccaag cttcaaattc cttcataaat tcttgttcgt
     8161 aactcatttt ttctccttat tctaatccac gaaaatagtc cgtatcaatc tcacggtagg
     8221 gctggatatc ctgaacgtat tccagtacac cttggaattc tccgttttca tcgtgtactg
     8281 cagcataggt gatgtggaca aacttgcctc gcgactcaga cttgaaccac atttcatact
     8341 tgtccttggt cccctcacga agtcccttca tgatagcctt gaccttgtcc aaatacttag
     8401 gcggatgaca gagttcgaca ttgcgcccga cttgggacgg cgtccgtttg aaaatcatct
     8461 catcagctgg cgtattgtca ttgtaatact ggaaaatatc ttctttattg acaaaggtaa
     8521 tctccatagg gagatgattg aggatgagat tggcctgctc gactgaaaga tagccattac
     8581 caaaagcctg ttgactatgg cggtccagca cagcttcctt ttccttaggg gtaaaggtaa
     8641 tggtaaaatg gccttctggc gtatctataa cttgttgaac ttgaccttct gccgtatcta
     8701 gctgtacagg ctcctctgca atcttttcct caataaagct ctgtcgttct ggcacccatt
     8761 tctctgacgg acggatgatg gcatagccat aggcatcgct ctcctccgca atctgaagcc
     8821 agtcatcctg agtaaaagac tcaaggagaa tcatgaggag gatggactct tccttgaaaa
     8881 tcatactttc aaactctatc gcaaaagctt caaaaacttc ctttacactg ctaattgaca
     8941 cttctggtag tgacttggct gtcgttagag ctgtttgaaa gagttcccta atctgatcat
     9001 ccactcccca cataactttg ggaggtgaat cgtgtccata gcgctccatg ataggaaaga
     9061 agagttcttc tttacgttgg taatggatgt caaattgccc cacaagtccc atctgacgca
     9121 ccaagccctt gcgcatctcc gcaagcattt cctcgtcttc catcaactca taggtatcta
     9181 acaatctacg aatgcgaatc aaagctgcac ggagggccaa attttcttcc ttaaagacac
     9241 gaactgggtg acctggatgc tcagtatctg aaacttcgac acctttgata gcatttttaa
     9301 aaagattggc atggacatca cagagttcca taacatcttc aaaggtgaca cctgagtctg
     9361 agttcataag ctcgtgctcc ataagggaaa tctcaatggc tgagacaccc gcaaaggtcg
     9421 catcaaaacg ctcctgaacc gactcaggag aggcgccatt atgcaattct aataaaatat
     9481 cccgtaggac atgaatccgt tcatctgcca ttagtctaat ccaatcactt cgtagccatt
     9541 cgcttccagt gtgcgtacaa tcttgtccat aggagttcct gctagcttag aaccctgttt
     9601 aagtgatact ttacgaccaa ctgtattgcg cattaaggga ttggcaaggg gtttaaaacc
     9661 caactccact agaatttcca agacttctgg atgcttgtcc accacttctg caacaggaat
     9721 tgacacatcg atgatattgt ccatgacgac ctccattgta tgttctaata ctcaatgaaa
     9781 atcaaagagc aaactaggaa gctagccgca ggttgctcaa aacagtgttt tgaggttgtg
     9841 gatagaactg acgaagtcag taacatatat acagcaaggt gaagctgacg tggtttgaag
     9901 agattttcga agagtataaa atgattttac tgcttatatt ataccatatg gaaagagaat
     9961 aaaaaactag cagtggagtt cctgctagtt ctctgcgact tggattacca tctagataag
    10021 tttaaagagc caatccaata tcttaaataa tatcttgtaa aacagcaatt ggactgcaaa
    10081 agaaatgatc atccaaaaga atttcacaat ctcaataatc ggtttgataa aaagaatagc
    10141 aagaagacca aaaaaatttc attctctctc ctctggctta atgagccacc gagccgtatc
    10201 cattagctta tctgcaataa agagttttcc cagaccaaca acgacatggt catctttctt
    10261 tatcgttttc atcactttta caccttgcac ggtcaaaaag taattaccat aaattttagc
    10321 tagagttttt atgagtccca tattactctc cttcgatgat ttcagcctct tttcggattg
    10381 tttcaacatc ttcttgatat tgaacttcac tatagttaac tagcttggac aattctttct
    10441 gcaagctttc acccatcggt ttcatagttg caaaggttaa accacctgaa atgataccac
    10501 ccaagatagg aataaatttc cccattcctt tggccagccc tcccttggta agattcacac
    10561 caaatattct taaaactttt ttcaaaataa gataccaaag cgtctttgtt aaagctttat
    10621 taggtactgt tttcattacc tgtttggcaa ttgttatacc accagcacgt agcaaagcag
    10681 cagttccatt cacccccaac atgacgccta gataaagcaa aagggtattt tgagcatctt
    10741 cactcaactc atcgcgtgaa gcccaaagat cctcataacc ataaatataa cctaattctt
    10801 gggccaattt cagagagaaa ccataaaatt gagccacatc agctggaatg gtaattgcca
    10861 tagcaagccc tccaggtaat cctgccaaaa cagaagtccc actcgctaat aaaacattat
    10921 ccctaataca ggcattagcc actcgatcta agatttcttg agataagaga gacgttggac
    10981 cttgttccaa taaggtagga atgtcctgtg gacccaattc tttggaaaac ttatccacta
    11041 aaaatttcga acgatcaacc ttaacaactg gtagttttac cacttgttgt aatacttgca
    11101 tagctaagtc ttgttcagcc atatacaaac tccttatttc ttttttacac cttatcatat
    11161 tatatttcct acaaaaaaag cattttctaa ttagataaca ttaaaaaata cagaacccct
    11221 ttt
//
LOCUS       DAVLYV010000059        11100 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLYV010000059 DAVLYV010000000
VERSION     DAVLYV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.059"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(47..1936)
                     /locus_tag="VYO70_001535"
     CDS             complement(47..1936)
                     /locus_tag="VYO70_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1713746.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVMDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2074..3639
                     /locus_tag="VYO70_001536"
     CDS             2074..3639
                     /locus_tag="VYO70_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1713747.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3639..4379
                     /locus_tag="VYO70_001537"
     CDS             3639..4379
                     /locus_tag="VYO70_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1713748.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEKIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLK"
     gene            complement(4488..4703)
                     /locus_tag="VYO70_001538"
     CDS             complement(4488..4703)
                     /locus_tag="VYO70_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713749.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VYO70_001539"
     CDS             complement(5574..6824)
                     /gene="ilvA"
                     /locus_tag="VYO70_001539"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1713750.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6874..7230)
                     /locus_tag="VYO70_001540"
     CDS             complement(6874..7230)
                     /locus_tag="VYO70_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1713751.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7468..7704)
                     /locus_tag="VYO70_001541"
     CDS             complement(7468..7704)
                     /locus_tag="VYO70_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713752.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VYO70_001542"
     CDS             complement(7811..8833)
                     /gene="ilvC"
                     /locus_tag="VYO70_001542"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1713753.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VYO70_001543"
     CDS             complement(8899..9375)
                     /gene="ilvN"
                     /locus_tag="VYO70_001543"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1713754.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9368..11068)
                     /locus_tag="VYO70_001544"
     CDS             complement(9368..11068)
                     /locus_tag="VYO70_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1713755.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
BASE COUNT         3351 a         2542 c         1917 g         3290 t
ORIGIN      
        1 tctatcttcc attatatcat actttttcag tctatggtag ctctctttaa aaggctccga
       61 tactgccgcc acctccgcct ccagagaagc caccgccaga acttccactt ccagaagata
      121 cggagtaggt acttgctgta tttgcgacac tagcataatg gctcatttgc gcgcttgaat
      181 gataaaacat actgtgccag ccataagcta catagagatt gatatctgga ttttcaactt
      241 gaatctgatg aaccttcatc aaatgactaa ccttgtccgc atagccaaat aaggttgcat
      301 agaccaagag acgattccaa acaacaatac tttccaattc agccttatcc agtcgtgcaa
      361 tctcacgtaa catattttca aaactggtcc agagatagta gacctcagct cccgcttcat
      421 ttagaacacc atcacgatta tctagtcgaa gcttccaata atagaaaaca gacaaaacta
      481 accctagaaa accaagtatt ggcaaaggga ggtaaagata gccatgaacg tctaaactgt
      541 acaagaacaa accaaatccg ataaataggg gcaggatagt caaggcaccc atacccactt
      601 gcaaggcctt ttccccacca gttaaaggac gataataatc tgggagcccc cagaaggaaa
      661 ctcgttttct cactccttct tgcatctggt tcaatacctc ttcaaaagaa gatttgagtt
      721 gaagccctct tgcttgaatc cgtttttcat cagaaacttt ggctctacga taaagactat
      781 cagatacctt gtaatccgca aacaaattgg aaagagtttc ttctttttta cctgaaaaag
      841 ctagatttag gcagtctctc tcaaagcttg acaaaccatc ttcttttact agcctcaaac
      901 caactgcatc tccttctgaa ataatagaaa cattcccacg gtccatcaca tctagcaagg
      961 tagcttgaat aagttggtca aaagtgaatt tgccagcccc cttgactaag ggactcactt
     1021 cctccaagga ggtcgagtag acagcctctg ataaaaccat aggctctaat tccattggtg
     1081 gttcatagag acgatgattt ttggcatatt tgactgaagg agtggtcttt cttctataaa
     1141 taaaatagaa gcagacactc aataacaagg agatagaaag gatcgaaggg aggacccaag
     1201 taacaagttg tttactctga tctttttctc taacaatcga gtcttctatc ttattaaact
     1261 cttctaaacg attccctttc aatccctgat ccctagcgct agcaaaatcg gtccgaggcc
     1321 agtaggcatg caactcaact ccacgcttag ccggaagatt gtctaaacgg atagtataat
     1381 caaggttact cttttcaatc gttccctctc taaaaagttt ccctgtatgg aaaaagagtt
     1441 tttcagcccc cttgtctccc cttacatgaa attcaaactt ttcaatagac tctgaactat
     1501 ctgtcagagg ttgccaattt aattcagcga tatcatcata aaggaaaagt aaatttttta
     1561 agttccagac gaggtcaact tcaactatgt cgccctcctg acctggatta tagactctca
     1621 cagtataacc atccgcttct tctgttactt cgctagtcac atctgctagt tctgcaccgt
     1681 ttttcgcggc ctgaaccttt ggatgagggt caatgtcaaa cccgctaggc atcttaccag
     1741 cacgtccaag tcccacgatt tgtcccttaa agtcctcctc aaactggtaa actatcttct
     1801 gtctaaactc tgccgtatta tctgcatgaa tatacaaatc cccttgataa gagtttatct
     1861 tgaaatcaat ggcaaaaaca gagagtggaa gaaggcaaaa caagcctaac accagtaaga
     1921 aaaaagtttt tttcatcaac taagccccct ttttatcttt cttatcatta tagcattttt
     1981 tcgtaagtaa aggcttactt atgttgaaaa attacgcatt tttcgataaa ataggagaca
     2041 gttatttttt aatagatcag aataggagaa atcatgagaa aaatatactt atctattttc
     2101 acaagtctct tgctgatgct aggacttgtc aatgttgctc aagccgatga atatttacgc
     2161 atcgggatgg aagcagcata tgctcccttt aactggaccc aggatgatga tagcaacgga
     2221 gctgtcaaaa tcgatgggac caatcagtat gccaacggat acgatgttca aatcgccaag
     2281 aaaatcgcta aggacttagg taaagaacct ttggttgtta aaaccaagtg ggaaggtcta
     2341 gtccctgccc ttacttctgg taagattgac atgattatcg caggtatgag tccaactgca
     2401 gaacgcaaac aagaaattgc cttttcgagc agttactaca ctagcgaacc agttttgctt
     2461 gtcaaaaaag attctgccta cgcaagtgct aaatctttgg atgactttaa cggtgcaaaa
     2521 atcacttctc aacaaggggt ctacctctac gacttgattg cacaaatccc aggtgctaaa
     2581 aaagaaacag ccatgggaga cttcgctcaa atgcgacaag ctcttgaggc tggtgtcatt
     2641 gatgcttatg tttctgaacg tccagaagca ctgactgctg aagctgcgaa ctctaagttc
     2701 aagatgattc aagtagaacc tggtttcaaa actggggaag aagatacagc tatcgctatc
     2761 gggcttcgta aaaatgacaa tcgtattagc caaatcaatg ccagcattga aaccatttca
     2821 aaagatgacc aagttgcctt gatggatcgt atgatcaagg aacaacctgc cgaagctaca
     2881 acaactgaag agactagcag tagtttcttt agccaagtcg ctaaaattct ttctgaaaac
     2941 tggcaacaac tcttgcgtgg tgctggtatc actcttttaa tctctatcgt cggaaccatc
     3001 ataggtctca ttattggact tgccattggt gtcttccgta ctgctcctct ctctgaaaac
     3061 aaagccattt atggccttca aaaactagtc ggctgggttc tcaatgtcta cattgaaatt
     3121 ttccgtggta cgccaatgat tgttcaatcg atggttatct actatggaac tgcccaagct
     3181 ttcgggatca accttgaccg tacactggct gctatcttca tcgtttcaat caataccggt
     3241 gcctacatga ctgaaatcgt ccgtggtggt atcctagcag ttgacaaggg acaatttgaa
     3301 gctgcgactg ctcttggtat gacccataac cagaccatgc gtaagattgt cctacctcag
     3361 gtagtccgta acatcctacc tgcaactggt aatgaatttg tcatcaatat caaagataca
     3421 tctgtattga acgttatctc tgttgtcgaa ctttatttct caggaaatac cgtggcaaca
     3481 caaacctatc aatacttcca gacatttaca atcatcgccg tgatttactt tgtcctcacc
     3541 ttcaccgtaa cacgtatcct acgctttatc gagcgcagaa tggacatgga tacctacact
     3601 acaggtgcta accaaatgca aacggaggat ttgaaataat gacacaagca atccttgaaa
     3661 ttaaacacct caaaaaatcc tatggacaaa acgaagtgct aaaagacatt tcactcactg
     3721 tccacaaggg agaggtcatc tctatcatcg gaagctctgg aagcggaaaa tcgaccttcc
     3781 tacgctccat taacctactt gaaacaccaa ctgatggaca aatcctttat catggacaaa
     3841 acgtcctcga aaaaggctat gacctcacgc aataccgtga aaagttgggg atggttttcc
     3901 aatcctttaa cctctttgaa aatctcaatg ttcttgaaaa cacaatcgtc gctcagacaa
     3961 ctgtcctaaa acgcgaacgc acagaagctg aaaagattgc caaagaaaac ctggaaaagg
     4021 tcggcatggg agaacgctac tggcaagcca aaccaaaaca actctcaggt ggtcaaaaac
     4081 aacgtgtggc catcgctcgt gccctctcca tgaatccgga cgccattctc tttgatgaac
     4141 caacatcagc tctcgatcca gaaatggttg gagaagtcct caaaatcatg caggacctgg
     4201 ctcaggaagg cttgaccatg attgtcgtaa cccatgaaat ggaatttgcc cgtgatgtct
     4261 ctcaccgtgt tatctttatg gataagggcg tgatcgctga agaaggtaaa ccagaagacc
     4321 tcttcaccaa tcctaaagaa gaccgaacaa aagagttcct tcaacgctat ctcaaataaa
     4381 aagaaaaggc tgcatcaacc gtgcagtctt tttgctgtcc tcaaaatgaa aaggcagatt
     4441 ctctatctct actacttgaa ggtcaagaat ccgccgttat ccaaagatta atcttcaaat
     4501 ttttcataat ttttttcata gaaatcaatt aggcctaggg ctgtttcaaa tgaaatattt
     4561 tttacttttg cacgaccctg cgccagagca atgatagaca tttcacgcgc attcgtttct
     4621 ttagagatac ggtagcctgt gattttctta tcacgaaccc agccaacaac tgattctact
     4681 ttttcaaagt ttgacttagc catgtttttc tcctattttc ttctttataa tatttaattg
     4741 tatacgtttt tactactttt gtcaataaaa aaaacatata ttcaacgaaa ataattagtt
     4801 cagaattttc ttacttcctt tttaaatccg ataatatata ggttttgcta gcctctacac
     4861 cataaatatt tgctaataaa actactgtat tattaccctc ttaaggtgcg aaattattca
     4921 agttcttaaa aaatgcttct ctatttccgt aagatagctt atttcttcga tttaagaact
     4981 gaagatgata gttctaacct ttagaaatcc aagccttcac tagctcaaaa actcctatct
     5041 ttcttcctca ataaatcatc tgagagatag gtattcctta tatccttcaa attcatcact
     5101 cctttggata ttgaggtgct agcctagaag cagacacccg attatctaaa ccatccttaa
     5161 tagagatgac aagacagata agctcagtta attacttata tatcttttta atatggtata
     5221 tttgtcaaca aaaagaaatc aaatcatacc ttatactcaa tgaaaatcaa agagcaaact
     5281 aggaagctag ccgcaagttg ctcaaaacac tgttttgagg ttgcagatag agctaacgtg
     5341 gtttgaagag attttcgaag agtattaaag acagtgtgac tatgcttgtc aatagatagg
     5401 aaatcaaaat gaaggatgga aatctgagcc aaacatcata agtgctataa tgaacagaag
     5461 taagaattgc acgataagtt ttctaaaacc ataggaatcg tcccctttct tcatcttcat
     5521 tatagcacat atacatcagt tgtacaaata atatgatatt tttttattag tcctcagaca
     5581 agcatattat aaagcgtttc attaccattt aagttaatat aagctggatc aaaaccttcc
     5641 attctacgaa tcaaacctgc ataatcatgc ttatctgcta aagcgatccc aattaatact
     5701 gggcctgttc ccttgctagc tcgtttgata tactcaaaac gtgtgatatc atcatttggc
     5761 cccaggatat catttacaaa ctcacgcaaa gctcctggac gttgtgggaa attgaccaca
     5821 aagtaatgtt tgataccatc ataaatcaag gcacgctctt ccatttctgg catacggttg
     5881 atatcattat ttcctccaga aatgatacaa caaatggttt tccccttaat atattcagct
     5941 aaaacctcta aagaggcgat actagccgct ccagcaggtt ctgcgactat cccttgctta
     6001 gagtaaaggt caatcaaggt ttcagaaatc aatccctcat cgacacctac caaagtttta
     6061 atatgttgac gagttgcttc ataggtcaac tgacctacct tttgcacagc aatcccatcc
     6121 gcaaatttat caatttcctt gagtttgact ggacctccag cctcaaaggc agctttcatg
     6181 gaacgcgctc cattcgcctc tactccgata acctcaatct ctggacttgt ttccttgata
     6241 taggtagaaa ccccggcaat gagaccgcca ccaccaacag gaaccaagac agcatcaaaa
     6301 tcaatcgatt cttttcgagc ttcttctaaa atctcataag caactgttcc ttgacctgct
     6361 tgaacatgag catcatcaaa aggatcaata aaggtacgat tttcagagac tgtaaattct
     6421 tgagctgctt tggctgaggc atcaaaggta tctccaacta gtttaatagt tacaaaatcc
     6481 ccaccaaaaa agcgaacctg accaatcttt tgttgtggcg tagtaatagg cataaagata
     6541 gtagcaggaa ttttcatttc attacaagta taggctactc cctgcgcatg atttcccgca
     6601 gaagcgcaga ctaccccacg ttcacgttct tccttgctga gctgggaaat ggcataatag
     6661 gcaccacgaa ttttaaagga gcgaacacgc tgggcatttt cttttttcaa ataaatctta
     6721 gcaccatact tctccgataa ataatgatcg taatccagtg gagtattcac aaccacaccg
     6781 ttcaagacct tgtgagcctt gattatatct tttgaactta acatataaaa cctctttttc
     6841 tataaaaaac agtcactaat gactgttttt tagttattca tctccgaaaa cttcagtaac
     6901 actttcatgg tatttatgag ccatacgaat cgaaaactct ttaggaagtt cataacgatt
     6961 tgtggaaatc caatcatata accattctac caaactttct tcactatcat tacgatggaa
     7021 gataaaatag tttaaaattt ctttatattc tacatcattc aaatccaacg tgtctacaaa
     7081 ttgactacga ggaacaaaat tagaatctaa ttttcttagt tgatctgtca attctcccaa
     7141 ttgtaaatcc aactgaaaaa atagtttttg cagttgccta tcagtttcat cacatcctgt
     7201 cttgatagaa aacaaattgg taatattcat atgatttcaa cccctaaata agtacatcta
     7261 atatcccacg aatagctgaa gctaaagttt tcgcagtcca gtcatttaaa cccgattctt
     7321 tcaatccaga tgcaataatt tgctctattt gatataaacc gccatctatc gtctcattta
     7381 ttctgcgaag aggaccttca atataggata aagcttctcc gaatctaaca gctgatttag
     7441 gtcctaatga tttaccaact atatttgtta atgtttttta tttctcaaaa tccatttcgc
     7501 tatataacga gcagatttag tcgcaccagc tctctcaaac ggagaatgaa tttcaagacc
     7561 ttcttctcta gctatttgtg ccaatttgaa gaattgttca aaggtcatat gcaacctacc
     7621 atcttcaaaa ttagcatcat cataaatgtt aattacagca ttttcatcat acgaccaatc
     7681 tgctttattt tggtctgcta gcactattac cgacggtgta attacattat acaactagaa
     7741 ccaataataa cgatgcaatt tttctcataa tctttttcat aaaatcatct ccagcgctat
     7801 atatttctaa ttagttatag attttgaatg catcatcgtc gtttttacca acgaatggca
     7861 ttgctttacg caattctgca ccaacttttt caatttcaag gttagctgct tgttcacggt
     7921 aagcagtcaa ttttggacgt ccagctttat agtcatttac aaagtcattt gcaaatttac
     7981 cattttggat gtctgccaag acagccttca tattttcttt aacttgttca gtgattacac
     8041 gtggacctga tacatagtca ccgtattcag cagtgtttga aatagattga cgcattttct
     8101 tgaatccacc ttcatagatc aagtcaacga tcaatttcat ttcgtgaaga acttcaaagt
     8161 aagccaattc tggagcgtaa cctgcttctg tcaagacttc gaaacctgct tcgataaggg
     8221 cagtcaaacc accacaaagt acagcttgtt caccaaacaa atcttcttca gtttcttctt
     8281 tgtaagttgt ttcaagaaga cctacacgag ccgctccaac acctttacac cagtccatag
     8341 caatgttttt agcatttcct gttgcatctt ggtatactgc ataaagagct ggaacaccaa
     8401 atccttcttc gtaagtacga cgtaccaagt gtcctggtcc tttaggagca cacatgaaga
     8461 catctacatc cgcaggaact ttgataaatt caaagtggat gttgaaacca tgggcaaatc
     8521 caactgcgtt tccagcttcc aagtttggag cgatttctgc ttcgtacaat tcttgttgaa
     8581 tttcgtctgg cgccaagatc atgataacat cagccaactt agtagcttct gctactgtgt
     8641 aagtatcaaa tccatcttct tttgctttat caaaagattt acctggacgt acacctataa
     8701 taacgtcacg acctgaatca cgcaagtttt gagcatgcgc atgcccttgt gaaccataac
     8761 cgataacggc gattttttta ccgtcaagtg ctgctacttt aacatctttt tcatattcca
     8821 tttgaactgt catagctttt ctctcttttc tattatttat tgccttttag gctggtttaa
     8881 taaatttaag ttggattttt aatcgcgggt aaatccagtt gcacccgttc gagcaatatt
     8941 gcgaataccg tatgggcgaa tgactctcaa tagggcttcg ctcttttctg catttcccgt
     9001 catctgaatg gtaatcgagc ttggcgctac gtctaccact gttgcacgga aaggttgaat
     9061 aatcgctaaa atctcagctc tcttctcagc tggcgctgac atcttaacca aaatcacctc
     9121 gcgctccaaa tgaggcttgt ctgtaatatc tcgaatgcga atcacatcaa tctgacgatt
     9181 gagctgtttg atgatttgct ccacttcatc atgagaagca acatcaataa taatagtgat
     9241 acgcgataca ttcggatctt ctgttgctcc aacagagatg ctttcaatat taacctgacg
     9301 acgagatagg acacctgtaa agcgattgag gactcctgaa cgattttgta gttttgctgt
     9361 taacattcta cgcatggaac tgcaccccca acatctcatg attactctta ccagccggta
     9421 ccattggtaa cacctgttcc ttacgagaaa tatctacctc aattagcata ggaacatcct
     9481 cagtgatgac ttcaaggtct tgagccaagg tctcaggatt gtcaaactta tagtttttaa
     9541 tgccataagc ctgcgccatc aattggaaat caggaagggt atcaaagacc gactctgatg
     9601 ttctgccttc atagaaggat tcctgccact ggcgaaccat tccgagtgaa tgattgttca
     9661 gcataaccac cttgattggc accttgtaaa tattcaaaat agccaactcc tggttagtca
     9721 tttggaaacc accatcccca acaaacaaga ctacttcctt atctgggtta gcaattttag
     9781 caccgattgc tgctggaatt ccaaagccca ttgttcccaa accacctgaa gtcactaact
     9841 gacgttcatt ttggtaggga taatactgag ctgtccacat ttggtgttga ccaacgtctg
     9901 ttaccacaat ggcatctcca ttcgtcaatt caccaattcg ttcaataact gcttgcggtt
     9961 gaaccacacg ctctttcttg tcataagaac gaacacgatt cttgtcttta gtgactttct
    10021 caatccactt ttcagtattg ttgtgaactg ttggttctgc tagcaacatt tgcaaggcct
    10081 tcttagcatc tccaactaca ggaatgtctg cactgataat cttgccaatc tcagctgggt
    10141 caatatcaat gtgggcaacc ttagcattct tagcgaaagt cttaggattc cccgtcaaac
    10201 ggtcatcgaa acgagaacca atactaatca taaagtccgc ttccgtcatg gcaatatttg
    10261 ctgcgaatga cccgtgcatg cctcccattc caagaaagag tgggtgactc gttgcaatcg
    10321 ttccttgtcc caaaagactg gttaccactg gaatttgata gcgttctgca aattcattta
    10381 gttccgtagc agcctcagca taactaattc caccaccagc taacaagact ggctttttag
    10441 ccttggacaa ttgcttcaag attttcttga tttgcatatc attcggctca agagtcggct
    10501 gataacttgg taaattcact tctggtgaat aaatgaagtc tgtttctaaa gcagatatgt
    10561 cttttggtag gtcaattaca actggccctg gacggcctgt agttgcgata tggacagctt
    10621 ccgtaatgat acgcggaata tcagctgtct cacgaacttg gtaattgtac ttagtgattg
    10681 gcatggtaat tcctacgatg tctgcctcct gaaaggcatc cttcccaatc cctgctcgcg
    10741 ccacctgacc tgtaaagacc aaaaggggaa cgctatcgct catagcatcc gcaatccctg
    10801 taatggcatt tgttgctcct ggtccactag tgacgacggc aacacccaac tttccagttg
    10861 atttggcata accttcagct tcatgcaaac aaccttgctc atggcgccct agaatgtggc
    10921 gaatgccttt aaaattatat atcgcatcat aaaaaggcaa aaccgcacca ccaggataac
    10981 caaagatggt atcaactcct aaatcacgaa gtgtttccaa aactaggtcc gaccccgtct
    11041 taggagattc taaactgatt ttctccattg ttcccctttc tttcctctta aaaataactt
//
LOCUS       DAVLYV010000060        10957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLYV010000060 DAVLYV010000000
VERSION     DAVLYV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.060"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            104..268
                     /locus_tag="VYO70_001545"
     CDS             104..268
                     /locus_tag="VYO70_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1713756.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            352..918
                     /gene="amaP"
                     /locus_tag="VYO70_001546"
     CDS             352..918
                     /gene="amaP"
                     /locus_tag="VYO70_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1713757.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            930..1100
                     /locus_tag="VYO70_001547"
     CDS             930..1100
                     /locus_tag="VYO70_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1713758.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1139..1747
                     /locus_tag="VYO70_001548"
     CDS             1139..1747
                     /locus_tag="VYO70_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1713759.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1778..1981
                     /locus_tag="VYO70_001549"
     CDS             1778..1981
                     /locus_tag="VYO70_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1713760.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2092..2364)
                     /locus_tag="VYO70_001550"
                     /pseudo
     CDS             complement(2092..2364)
                     /locus_tag="VYO70_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2673..2960
                     /locus_tag="VYO70_001551"
     CDS             2673..2960
                     /locus_tag="VYO70_001551"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1713761.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3238..4008)
                     /locus_tag="VYO70_001552"
     CDS             complement(3238..4008)
                     /locus_tag="VYO70_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1713762.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(4023..4871)
                     /locus_tag="VYO70_001553"
     CDS             complement(4023..4871)
                     /locus_tag="VYO70_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1713763.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGTPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4868..6343)
                     /locus_tag="VYO70_001554"
     CDS             complement(4868..6343)
                     /locus_tag="VYO70_001554"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1713764.1"
                     /translation="MVQTKQPNIILIVVDQMRADVLSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSVHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6666..8021)
                     /locus_tag="VYO70_001555"
     CDS             complement(6666..8021)
                     /locus_tag="VYO70_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1713765.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(8031..8348)
                     /locus_tag="VYO70_001556"
     CDS             complement(8031..8348)
                     /locus_tag="VYO70_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1713766.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTKEAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8365..8682)
                     /locus_tag="VYO70_001557"
     CDS             complement(8365..8682)
                     /locus_tag="VYO70_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1713767.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8697..9593)
                     /locus_tag="VYO70_001558"
     CDS             complement(8697..9593)
                     /locus_tag="VYO70_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1713768.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9590..10858)
                     /locus_tag="VYO70_001559"
     CDS             complement(9590..10858)
                     /locus_tag="VYO70_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1713769.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLEDCE"
BASE COUNT         3768 a         2200 c         1697 g         3292 t
ORIGIN      
        1 tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt gctggaggtc
       61 ttattggtct cttggcaggt aaaatcacta aaaaagtagt tctatgggaa tcatcgcaaa
      121 tgtattcgct ggtttagtcg gggcatatgc aggacaatct cttttaggta gttggggtcc
      181 atcaatcgct ggaatggctt tgctcccatc tattgtaggt gcagcgattg tgattactgt
      241 agtgtcattc tttacaggta gaaagtaaac ttttcgccag taaagttagc aaactatttt
      301 taaatcaatg acgggaaaaa tagtttaaat gttaaatcga aaggattgta tatgtcaaaa
      361 gcaaagaaaa tatgtttcat tattttctgt attttaatct tgacaatttt ccttcctgtt
      421 ttgatagatt atcatcaagt tagtgatcta ggtattcatc tacttagctg gagacagaac
      481 tccgtagttg aattctatct tgctagatat gtcttttggg ggacagtggt tctatcaact
      541 ttagttttat tatccatttt agttgtgatg ttttatccta aacgttactt ggaaatccaa
      601 cttgaaacta aaaacgatac attaaaatta aagaattcgg caatcgaagg ttttgttaga
      661 agtttggtga gtgatcatag attgatcaag aacccaactg ttcatgtaaa tttacgaaaa
      721 aataaatgtt tcgttcatgt agaaggtaaa attcttcctt cagacaacat cgctgacaga
      781 tgccaaataa ttcaaaatga aataactaat ggattgaagc agttttttgg tattgagcgt
      841 caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa aacctcaaaa caaaaagact
      901 gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct taaacaatat cgatatccaa
      961 ttatcgctgg tctcataggc gtatttctgg cttgtttgat tgtctccttt ggcttcttca
     1021 aaacaatatt tgtattgatt ttaggagcac tgggagttgc agctggatta tatatcgaaa
     1081 aaaactatat agataaataa aaaaataaaa attactaatt taattaaagg agtttcatat
     1141 gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg ttgtagctca
     1201 cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca ttggtctttc
     1261 actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa atcttaaaga
     1321 aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg gtaaaacaca
     1381 agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag ctttatattc
     1441 agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg aaattgttga
     1501 aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag actcagtaag
     1561 ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt cagaacaatt
     1621 cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa aagttagcga
     1681 aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa acactcgtgt
     1741 aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag aaaaattaaa
     1801 tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg aaaaaatgga
     1861 aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa gaagttgccg aagacgctaa
     1921 agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg acgataaata
     1981 aggttaaaag ttactttatc ttttttggta acattagtca aaagagtctg agtcaagatg
     2041 attctcagaa aacaaaaagc tagagattcc caattgcgga actctagctt tttaattttg
     2101 cctctttctc ttattatatt tcagcaggtt gttggccatg agtatgaatc ccatgtcaat
     2161 tctcacttga cgcttacctc tcagatgaca tctcttataa cccaaacaaa cctttatctg
     2221 cccaaagaca gatttcatat caatcttacg tttagcgaaa atttgtctac ccttggaaga
     2281 taaaagtgcc tgatattctt tagtttttaa acactggtaa cgttcattca tatacagtcc
     2341 cttttgaggg gctgattcag gttcataatc gcagtcaaca ttgatttcaa ggctgtttgc
     2401 tttctatctc ccctttttca taatgtatag taaaatgaaa taataacagg acgaatcgat
     2461 cgggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt
     2521 caatctacta tattttcgta caggtgcttc aatagtttct atagtaaaat gaaccaaaaa
     2581 tagtacacgt tgcggtataa tgttcttgtg gcatattcat tagattttcg taaaaagttc
     2641 tctcctattt gtgagcgaac aggtatagtc aattgattgt cagtttgaag accaagagtc
     2701 gtaaggcaaa ggatatggca gaatccatcc aagggtggct ggcacaattt ctagtgaacc
     2761 tctttaaatc tattacgttt gactgtggaa aagagttttc aaaatggaaa gatatttcca
     2821 atcatcacga tagtgagagc ttttttgcga atctaggatg tccaagacag cggtgtctca
     2881 acgaacattc aaatagactt ttaaggtgtc atgacttgcc aaagcagaca gatttcaacg
     2941 aagtcagtca agaattttag gctgggtaaa aactcaattt caggagaaaa tgaaataaat
     3001 attcccacaa taaaacgcat agtatcaaag tttttccaac acctgatatt atacattttt
     3061 taattttaaa gactttttcc cagcctcaag aaatcacatc ccacctaaat cctttggata
     3121 ccaaatacct catcaggtat tttctgagtt ggtaagactg tctagcttaa tttgacaaat
     3181 ggagggcgtt aaatccagtt tttacaggaa gtctatttaa atattttcac actgacctta
     3241 caaaatatga aataaattat ataaagtcat tcccatgaca aagaataaca tccctttaaa
     3301 acagtatctt acccaatttt caggtaagac ttttgataca accgtcccca atacccctcc
     3361 gcatatagaa gcaagaaaaa taactggaac aggtgctaaa tgatactgat ttaaacctgt
     3421 aaccacaaca attgtagcca aatgagacaa ctgggaaaag aaaataattg caagagaata
     3481 catcgttgct tcttttatcg aaatagaaaa aaacaccatc aataaagata cattgagcgg
     3541 accaccacca atccctaaaa aactagaaat caagcccaat agaagaccac aaatcatata
     3601 gaatcctatc ttatcaaatt gataagtttt tttaaaatct gttaaaacaa atgatacaag
     3661 tagcatcaca aatagtaaaa tcatttgaac aatggatact agccgtactg ataactgaga
     3721 taacaaaact tgaaaaataa gatgacctag aaatcctcct aaaacagaag aaaaggatac
     3781 cgtaaaaatc aatctccatt ttatctcttt actttgtgaa aatcttttga ttgttgaaat
     3841 aatcgccatt ataaaaacag agaaactaga ataaaaagca atatcactga ccgaatggta
     3901 tccaaaacta tccataagtg gcttaattat aattccacca cccatgcctg atatagaacc
     3961 caatccacta gctattataa tgattagaaa ataaataatc tcaaccactt tttaagtcca
     4021 tcctattctt tcaaacatct aaaacctaga tggctactag tggaagtaga aatacatcca
     4081 tttctagcag ctactcggta tcgattacaa taagaacaat gacataaaaa ggaacctcct
     4141 ctgatagcat actcctcatc ttgtatccca tattttggta actcatagtc atcttcatta
     4201 aaactagcta ataaggtata tctcggattg cgacaccatt cccaaacatt cccaatcatt
     4261 tgatatagac caaaatcatt aggtaaaaat tcatagacag gagccgttcc tataaatcca
     4321 tctaaggcag tattttcata aggaaatctt ccttgccaag tattggcatg ataatatcca
     4381 ccttctaaaa gagtatctcc ccaaggatat tcagattgtc ttcctcctct agcagcatat
     4441 tcccactgtg cttcagtcgg taaagacatt ccgctccaat tacagaaagc caatgcatct
     4501 tctaaagcaa catgaactac tggatggtct tctaaaccca ctagattact attctcaccg
     4561 tagggatgtt tccagcaagc gttcggaact tgtaaccacc acggagtccc agcaggatga
     4621 ggatatccct ccctttctgc ctctggaacg aacaatataa aaacaaaaga ccactcttgt
     4681 cgctcagcta gtgttttata gcctgtttct ttcacaaatt gagcaaattc ttgattcgta
     4741 actggagtat ccgcaatgga aaaatccttt acactaacag cgacttgagg accttcaaaa
     4801 tcatctaaaa agccctcttc agaatttgtt cccatcataa atgtcccacc cttgattttt
     4861 ttcattttca tgattgacta tcccccgttt tcgttagagt tggcgttatt ttagaaagtt
     4921 caacaggaac taactcttca tccacaacaa aaccttcttc tctatatcta agaaagtcta
     4981 ctaatttttt cttcatctga cgaacaatag gttggtattt ttcacttgga tacaaatcat
     5041 tcatttcatg cggatctttt ttcatatcaa ataactgata atgatttaaa acaggaaacc
     5101 agataaattt ccactggtct gtcaagatat attgactaga atcttttcct aaagcatgtt
     5161 caccatgaaa ttctgttcgc cacccctcgt actgtccaaa taataaattc ttaacactcc
     5221 gtccatctag ttcatctgta gtagttcctc ctgccaaatc cacgagtgaa ggaaaaatat
     5281 cttgaatttt cactaattgc ttgattgttc ccctattacc agctatcaat ccagccggat
     5341 cgtaaataaa cgaaggaatg tggacacttc cctgataagg atatcccttt ctaaacagat
     5401 aatgttcacc aagctgatct ccatgatccg aaacaaacca gataatcgta tctttatcat
     5461 gacgaaattc ttttagggca gtcaaaaagc gactaatttg atggtctata tgagtaatca
     5521 atccaaaata agcagcaacc attcttcttt gatcatcttc ctttaatttc cctcttaaag
     5581 cataaataga cggaatttgc ttttctaata cttcccaatt cccgatatgc aaatcgagaa
     5641 attgaggcaa gcgttccata tacatatcaa aatagtattt tgggggattt aatggagcat
     5701 gtggtttttc aaaagacatt ttgagaaaaa aaggaaccgt tggatctctt ctttgtaaaa
     5761 atgaaatgga ctcactcaca acccagtttg ttggatgtaa cttctcatct ttatcccaag
     5821 gtctagcttc ccaagaatta caatccattc cgtcatcaat caaatcaaca tcataaccaa
     5881 ctttaccctt taaaaaggct agatagtcac tagcataatc aaattgactt ccataagcct
     5941 tatcatactt tctatcaaca tgtagatacc catcgtgaag taagacatga tcaaacccaa
     6001 gtctctgtct ggaaggaaaa acatgcatct taccaataca ctctgtttga taacccatat
     6061 ctttaaagac ttttggaaga gtattagtaa aattccaagg cacctcatct tgataaccaa
     6121 ctcgtccact tttatcttgg tctaagccag tcaataaagc tgccctagca ggaacacaag
     6181 aaggaactgg agaataggca ttttcaaaat tataacctac actagccatc atatctaatg
     6241 ttggagtgct aactagctta tccttactat tcaaggataa gacgtctgct ctcatttgat
     6301 ctacaacaat caaaataata tttggctgtt ttgtctgaac cataaaatct cctttctaat
     6361 atggcaaaag aggcacaaga agatatctac ctttactgca cccctttcta tatcaatctc
     6421 tctatataaa acaataacat tcttgttatg ttttatagaa aaatgaacta aaatatgact
     6481 aaatcgatta ggaaattcaa atcattttct agtactgttt tagaagttac agtgtactat
     6541 tccaacttca ataaattata aacctttgtc taataacaat tttagtggag ataagaaatc
     6601 ctacacctaa ctcatcttac acgtaatcta tttctatttt atcacaaaaa acgcaagtaa
     6661 gaccattaac tcaattcagt tttatctgcc attttcacaa atgggaaata agtcaagaca
     6721 ctaataatca aacaaacaac tgataagatg atggcacgcc aatcaaatgc tgtagagaag
     6781 aaaccatata aaattggagg cattacccaa gtaacgtttt gtgtaacagg tgaaacaaga
     6841 ccccagcttg ttgcccagta agctaccgtt gccatgaaaa ccgggctaag tacaaatggt
     6901 ataaatagca aaggattcaa gacaactggt aaaccataat tgaccggctc accaatatta
     6961 aacagaactg gtgctagacc aagtttagca acttttcgat aatgactgtt tcttgaaaaa
     7021 attaaaatag caagtactaa tcctaatcct ccaaaccaga caaacgcccc aaaagaccca
     7081 cttgtccata tataaggaat cggttcacct ttttggaaag catccagatt cgctaacata
     7141 gcaactccaa acagcccttc catgatggga gccaatacat ttcctccatg gagaccaaaa
     7201 aaccagaata acttattcaa aaagatcatc agaataactg caaagaaact ttgagacaaa
     7261 cctagtaatg gcgtttgtaa caccttgtaa acccaatcaa tcaataagtc attgctaagt
     7321 aaatggaaaa cataagtcaa gatggctact atatacatcg ccataaatcc tggaatgata
     7381 gaagtgaacg gcttagcaat cgcaggggga actgaatctg gtaacttgat tacccagttc
     7441 tttttcatta ctttacagaa aataatagag gctaaaaatc caatcatcat ggctgtaaag
     7501 tagcctctgg cattaatatg gtttcctgga atcacattcc caatagttac catcagattt
     7561 ttaccatcaa atgctagatt atcaattcct tgtaagattt gatctaattt cacatctcct
     7621 acatttgcca aagggaaact ctttgtaact gtacttccaa tcgaaatgac aaacgaagca
     7681 agcgatacca aaccagcaga aactgtatca accttataaa tcttagcgat attcactccc
     7741 aagcaataga tgaacaataa ggaaacaatt ggtatacttc ccttgaatac caaattattg
     7801 atgtcaacaa gccactgaaa ggttttcgta atactttcta ggtgaaattg ttgtggtaaa
     7861 tccactagaa aagcatttaa taacaaagca atggaacctg tcataataac aggcatagtc
     7921 cccacaaatg aatcacgtaa agcaactaaa aacttctgat tcccaacttt cattgcaatt
     7981 ggagaaagtt tttcttgcat tttatccaac attgtattca tagcaaattc ctaatttttt
     8041 tcaaatcgtt cgtatacttc taccacttct ttcgccaagt caacaaaggt cagactcgtc
     8101 attaaatgat cttgcgcgtg taccatcaag aggctaattg atgtttcttc cccctgtgcc
     8161 tcttttgtca acatttctgt ttgagcctta tgcgcctgta ataaggcagc attcgcatca
     8221 gctaatcttc gattggcttt agagaaatcc cctttttttg ctgcctgaat agcctccata
     8281 gcattacttt tggcttcccc accatacata attagcccca ttgcagattc taaattactt
     8341 tcattcatag cattctcctc aatcttagtc taataaactt tcagctaaat ctaagacttt
     8401 agaaccattc atcattccat aatctgtcat cggaataacc gccacaggaa tctgtctatc
     8461 ttttagtttt tcttgaaagt cccctagtaa atagcgaact tgagggccta aaagcaacac
     8521 atttacttct tttgttgcta caatttcttc tgcttcagga gctggaactg caaaaatttc
     8581 tgcatccaac cccttatctt ctgctgcctt ttgcatcttt gtcactagca gacttgtact
     8641 catacctgct gcacatgcta acataattgt tactttagcc atagcttact cctttatcac
     8701 ctatttactg acctagccaa gcgagatact gtatgtctca ctcctctaat tgccttgctc
     8761 aatgcataaa tattatcgac tgttgccagt cccccatatc ctgcgtcacc tatatgttga
     8821 atatcaactc cacaaatttt atttctaagt gcaatttcct taatagtatc tgtatcagat
     8881 gtttcttggc tagtaccaat agcactcaat acaagaccac ccttactatg aacgagatca
     8941 acgacttcac gcaactcttg gtcatgaaag gctggaacgg ttccaactgc tggaacaagt
     9001 atcacatccg cacctgcttc caacaattgc tctgcgacag aaagctctgc cacaggctca
     9061 ttcactcctg ccccgtgcat cttacctgca ataatcaaac cagaaaagtt ttccttagca
     9121 gtttgaacag ccttaatgat ttctcgattg ctaactcctg ttccaggatt tccagtcaaa
     9181 cagacaaagt caaaacctaa ctcctctatt cgcttcaatg tttcaacact agcaacacga
     9241 cctgcaacaa tttcctgtgt ttcctctagc atctttgcag atgggtcaat cggttccaaa
     9301 tttacaccaa ttggacaagc aacaagctgg tgtaagcgat gaataacatc atcacctgaa
     9361 ctatccaaag cataaatttt aggctcaaaa acatcaacac aattcaataa aatcatatca
     9421 gctccaaaag ctctagcaat ttcagaatta gttatatccc caacaaatgt ctcacgagta
     9481 actacatttt cagatagaac aactctacct tcacttgcta aaatactttg ttttaattct
     9541 tcagcattca tctgtaatat ctcagatgga tttgcactaa ttaaacgttt cattcgcaat
     9601 cctccaataa cttaaaccgt ttttcaatag ctattacaaa tggtaaataa agtaagacat
     9661 ccaaaacaat caatacaatt tgtaataatg aaccagaaat acttcctgta gcaagtaaac
     9721 cacttataac aggtggcata gtccaacctg gatctgtata agttagcgga accaaacctg
     9781 atgccattgc tccccacgct actagtaagt taaacattgg agctaagata aatggaacta
     9841 gtaacaaaat gttcaaaaca ataggaacgc caaacattgc tggttcattt atattaaata
     9901 atccaggtat aacagttata ggtgccaaag tttttagttg tttactagct ttcttcttat
     9961 gtgctaaata tcctaacgca agaactaaac ctatggttgc accaccgcca cctatgaata
    10021 caaaattatc cataaatacg ctagtaaaaa tatttggaag agtttctcca gtttgataag
    10081 ctactttatt tgcatctaga ttagctaacc ataacggttt cataactgca ttaacaacat
    10141 ttcctccgtg taatcctaca aaccaaaaac tactattaag gaaaattaag ataagaaggc
    10201 caataacatt atttcctaaa agtccaagtg gacctcccaa agctacttga gcaatttcat
    10261 gaagattagg taaacttaat ttatctaaag ttgcataaac aatcaaccaa ccaacaatag
    10321 tcactgcacc aggaataatg gcactaaagc ttttagatac tgctggtgga acaccatccg
    10381 gcatttttat ctgtatatta tgatttataa accattggta gatatagcca ttaatcaatc
    10441 ctaaaatcat agcaacaaat aaaccttttg atgccatgaa agcggtaggc attcctgctc
    10501 cagcctctcc tctaatgaaa ggcgtaactg taataaaaga tgataatgac acaataccag
    10561 caggaatacc atcaactttg tattgtctag ccataaagta agcgatacca aaactagcta
    10621 caagaccaag taatccaaaa ctactatcaa cacccttcca taaatagtca gcaacaccaa
    10681 tatcacccag atatttttcc catcctggaa taggaaaact tgcaataacc attagtaatg
    10741 aacctatcaa caataaaggt atagttagag taataccgtc tcgaatagaa accaacgctt
    10801 tattattacc caatctagcc gctactggaa gtaacttttc ttgtaataat ttttccataa
    10861 ccttttcctc ttaaatatat tttattatca aaagttatca attatcttta ttttttgacg
    10921 tcaagttgtt atttgattat cttttgttat ttatatt
//
LOCUS       DAVLYV010000061        10922 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLYV010000061 DAVLYV010000000
VERSION     DAVLYV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.061"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            123..986
                     /locus_tag="VYO70_001560"
                     /pseudo
     CDS             123..986
                     /locus_tag="VYO70_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1369..1989
                     /locus_tag="VYO70_001561"
     CDS             1369..1989
                     /locus_tag="VYO70_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713770.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            2075..2518
                     /gene="tsaE"
                     /locus_tag="VYO70_001562"
     CDS             2075..2518
                     /gene="tsaE"
                     /locus_tag="VYO70_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1713771.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            2508..3026
                     /locus_tag="VYO70_001563"
     CDS             2508..3026
                     /locus_tag="VYO70_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713772.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVCHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLID"
     gene            3034..4050
                     /locus_tag="VYO70_001564"
     CDS             3034..4050
                     /locus_tag="VYO70_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1713773.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            4135..5391
                     /locus_tag="VYO70_001565"
     CDS             4135..5391
                     /locus_tag="VYO70_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1713774.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGASSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQEQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            5446..6612
                     /gene="recA"
                     /locus_tag="VYO70_001566"
     CDS             5446..6612
                     /gene="recA"
                     /locus_tag="VYO70_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1713775.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            6919..8289
                     /locus_tag="VYO70_001567"
     CDS             6919..8289
                     /locus_tag="VYO70_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1713776.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQSSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKQPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            8665..9621
                     /gene="lytA"
                     /locus_tag="VYO70_001568"
     CDS             8665..9621
                     /gene="lytA"
                     /locus_tag="VYO70_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1713777.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(10254..10922)
                     /locus_tag="VYO70_001569"
                     /pseudo
     CDS             complement(10254..10922)
                     /locus_tag="VYO70_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         3307 a         1882 c         2386 g         3347 t
ORIGIN      
        1 ttatgtaatt tttgactttt tttagtttat taccataaag aattatttat ttctttaact
       61 tgctattttg aactaaaaat tttataatgc aattaagcaa ataggaaggt ttattatctt
      121 taatgagtac actcgcagag aaattcagat tgaaaagaaa agagttgaga ctctcccaac
      181 aaactcttgc agaaggaatt tgtgaacaaa gccagattag taaaattgag agagggcatt
      241 tcattccctc cgcagacctt ttgttcaaac tctctcaacg acttgaagta ccattagatt
      301 atttttttaa tgaacaaatt gaaattaaat ctaacctctc taatttcaag caattgtctg
      361 ctcgactatt agacgataga aattatgacg atttggaata tatttataga atagagattg
      421 aacgaagtac ttttctaaca ctagaagacc gaacttacct tgaatggatt aaagctatta
      481 ttgacttcta tcaatatgac agtaagtgtg aggcaatttc ttcattggaa aatatattat
      541 taaaagtctc ttcaaatact ctgatttatt taaaggcgtt gaatactcta tctaatttct
      601 attccttagt gggtcgtgaa caagaatatg aggcaaacta ctctcattta atagagttgt
      661 atcagacaaa aaatttggac catcaagagt ttttatttgg ctacatcaga gttcgttaca
      721 actacgctca ctacctagta tcaaaggaaa aatataatga agccatctaa gaagctcttg
      781 aaacgattga actctgtaaa caaagataga caagctacca attggctcct ctacttattc
      841 ttgtaggaaa tgctggagcc aaatttctag acagagaaca agtcaaaaat tattatatag
      901 aagcaagaga gctatgcaag atttataaca atcctttaat gctgatgaag atagaaaatt
      961 atttgaagga actagatgct gtttagttta tcttgatatt ggagtgttac ttaaacatta
     1021 aatagcctgt aaggctgata gttagattaa tactatcagc cttataatta tgtgactctg
     1081 agtccgtctc gctccgtgag gtgcgagaca aaacaaccac ccgtttgacg gattttcatg
     1141 atttcaaatt tatattcttc gaaaatctct tcaaaccacg tcagctctat ctgcaatctc
     1201 aaaacagtgt tttgagcaac ctgtggctag ctacctagtt tgatctttga ttttcattga
     1261 gtattagtct ctagtgtttg tttagcgtct taaagagaca tttgagaaga ttagaaacag
     1321 ttctctcttt tctcctagta taatgattgg taggaaggga gagagaagat gaaatcaatg
     1381 agaatcttat ttttgttagc tttaattcaa atcagtttga gtagctgttt cctatggaag
     1441 gaatgcatct tgtcctttaa acaaagtaca gcttttttca tcggaagcat ggttttcgtt
     1501 tcaggaatct gtgctggagt aaattatctt tatacccgta agcaagaagt ccatagtgtc
     1561 ctagccagta agaagtcggt gaagcttttt tacagtatgt tactcttaat taatttgtta
     1621 ggagctgttc ttgttttgtc agataacttg ttcatcaaaa atacgctgca gcaagaatta
     1681 gttgactttt tattgccatc cttctttttc ctatttgggc tagatttgct gattttttta
     1741 cccttgaaaa aatacgtgcg cgattttctt gctatgctag acagaaaaaa gacagtgttg
     1801 gtgactattt tagcaacact tcttttctta agaaatccaa tgaccattgt ctcacttctg
     1861 atttatattg gattgggctt gttttttgca gcctatcttg tcccaaattc ggttaagaag
     1921 gaagtttcct tttatggtca tattttccga gatcttgtat tggtcattgt tacgctcatt
     1981 ttcttttaga gaaagcctgt tttttatgga tgttatgatg ataagtttag taggaaaata
     2041 gacacaaaag aaaagttttt ggtataataa gagtatgtac acaaaaaatg aagaagagtt
     2101 gcaagcctta ggggagcgtt tgggccatct attagcaaag aatgatgttt taatcttaac
     2161 tggagaactg ggtgcaggta aaacgacctt tactaaagga cttgcaaaag gattacagat
     2221 ttctcaaatg attaaaagtc ctacctatac tatcgtgaga gagtatgaag gtcgacttcc
     2281 actttatcac ctagatgttt atcgtattga aggagatgct gattctatcg acttggatga
     2341 gtttatcttt ggtggcggcg tgactgttat tgagtgggga aatctcttag gagatgcctt
     2401 gccagatgct tatttagaat tggaaattct aaaagaagca gatggacgcc gtttaaattt
     2461 tcaggcaaag ggtttgcgtg ctgagaaatt gttagaggag cttcaatatg gagtatgaat
     2521 tgctcattag ggaagcagag cccaaagatg cagctgaatt agtggccttt ttaaatcgtg
     2581 tgagtttgga gacagacttt accagcctag acggagatgg tattctcttg actagtgagg
     2641 agatggaaat attcctcaac aagcaagcta gttcggacaa tcagataacc ttacttgcat
     2701 tcttaaatgg caaaattgct ggtattgtaa atattacagc tgaccagcgt aagagagtct
     2761 gtcatattgg agatctcttc attgtgattg gaaaaagata ttggaataat ggcttgggaa
     2821 gtttgttgct agaagaagcg atagagtggg cacaagcaag tggcattctg cgtcgtctcc
     2881 aactgactgt ccaaactcgt aatcaagcag cagtccatct ttatcaaaag catggctttg
     2941 tcattgaagg tagccaagag cgtggggcat atatagaaga agggaaattt atcgatgttt
     3001 acttgatggg taaactgata gattagtaga aatatggtta aaaaaattat tggaatggtg
     3061 ctagctttac tttctgtaac tgtagtagga gtaggtgttt ttgcttatac tatttatcaa
     3121 caagggacag aaaccttagc taaaacctat aaaaaaatcg gcgaagaaac caaggttatt
     3181 gaagcgactg aacctctaac cattctgtta atgggagtgg acaccggaaa tgctgaacga
     3241 actgaaactt gggtcggtag aagtgatagc atgatcttga tgacagtgaa tcctaaaacg
     3301 aaaaaaacaa caatgatgag tttagagcgg gatattctga cgcgcattga atcagggaat
     3361 ggtcaggctc atgaagcgaa actgaactca gcatatgcag atggtggagc agagcttgct
     3421 atagaaacca ttcaaaaaat gatgaatatc catattgatc gctatgtgat ggtcaatatg
     3481 agagggttgc aaaaactagt ggatgcagta ggaggtatta cagtcaataa tatcctaggt
     3541 ttcccaattt ctatcagtga ccaagaagaa tttaatacta tttctatcgg tgttggggag
     3601 caacatattg ggggagaaga agccctagtc tatgcacgaa tgcgttacca agatcctgag
     3661 ggggattatg gtcgtcaaaa acgtcaacgt gaagttattc aaaaagtcat ggaaaaagct
     3721 ctcagtttaa atagcgttgg tcattatcaa gagattctaa aagctttgag tgacaatatg
     3781 cagaccaata ttgatttgtc tgcaaaaagt atccctaact tgctaggcta taaagattca
     3841 tttaaaacca ttgaaactca gcagttgcag ggtgaaggag agatacttca aggtgtttct
     3901 taccagattg tttcgagagc acatatgttg gaaatgcaaa atatactccg acgttctttg
     3961 ggacaagaag aagttactca gcttgaaacc aatgcggttt tatttgaaga tttatttggc
     4021 agagcacctg ttggtgatga agataattaa cttttgatac aaataaaaaa atcaatcgta
     4081 ggaaaattcc tgcgattttt tcaaaaaaat acgaatagat aggtaggagg aaacatgaaa
     4141 gcagaaatca ttgctgttgg aacagagatt ttgacaggac agattgtcaa caccaatgcc
     4201 cagtttttgt cggaaaaact agctgagatt ggggtagatg tatattttca gacggctgta
     4261 ggagacaatg aagttcgtct cttgtctttg cttgagattg ccagtcaacg tagcagtctg
     4321 gtgattttga caggcggttt ggggccaact gaggacgacc taaccaaaca aaccctagct
     4381 aaatttttag ggaaagcatt agtctttgat cctcaggctc aggagaagtt ggatatcttt
     4441 tttgccctgc gaccagacta tgcccgaaca ccgaataacg aaagacaagc tcaaattgta
     4501 gaaggagcga ttccactgcc aaacgaaaca ggactggctg ttggaggaaa attagaagta
     4561 gacggagtga cctatgtcgt ccttccaggt ccgccaagtg aattgaaacc catggtctta
     4621 aaccaacttc tacccaagtt gatgacaggg agcaagctgt attcccgagt tcttcgtttc
     4681 tttgggattg gcgagagcca gttggttacg attttggctg atttaattga taatcagatc
     4741 gatcctacct tggcccctta tgccaagaca ggagaagtca ctctacgtct gtcaacaaag
     4801 gctagcagtc aagaagaggc gaatcaagcg ctggatatct tggaaaatca aatcttggac
     4861 tgccagactt tcgaaggaat ttctttacga gacttttgct atggttatgg ggaagaaact
     4921 agtttagcca gcattgtggt agaagaactg aaaaggcaag ggaaaaccat cgcggctgca
     4981 gagagtttaa cggcaggtct tttccaagct accgtggcga atttttctgg agcttcaagt
     5041 atatttaagg gtggttttgt gacctatagc ttggaggaaa aatcaaggat gttggatatt
     5101 cctgccaaga atttggaaga acatggtgtg gtgtctgaat ttacagctca gaagatggct
     5161 gagcaggcac gaagcaagac ccagtctgat tttggaatta gtttgaccgg agtggcagga
     5221 ccagatagcc tagaaggaca cccagttggg acagtcttca taggcttggc gcaagagcaa
     5281 ggaactgagg ttatcaaggt taatattgga ggcagaagcc gagcagatgt acgtcatatt
     5341 gcggttatgc atgcctttaa cctagttcgc aaggctttat taagtgacta acttttgata
     5401 taatagtaga taggtctgag gatcattaga atgtaggaga atagaatggc gaaaaaacca
     5461 aaaaaattag aagaaatttc aaaaaaattt ggggcagaac gtgaaaaggc cttgaatgat
     5521 gctcttaaat tgattgagaa agactttggt aaaggatcaa tcatgcgttt gggtgaacgt
     5581 gcggagcaaa aggtgcaagt gatgagctca ggttctttag ctcttgacat tgcccttggc
     5641 tcaggtggtt atcctaaggg acgtatcatc gaaatctatg gcccagagtc atctggtaag
     5701 acaacggttg cccttcatgc agttgcacaa gcgcaaaaag aaggtgggat tgctgccttt
     5761 atcgatgcgg aacatgccct tgatccagct tatgctgcgg cccttggtgt caatattgac
     5821 gaattactct tgtctcaacc agactcagga gagcaaggtc ttgagattgc gggaaaattg
     5881 attgactcag gtgcagttga tcttgtcgta gtcgactcag ttgctgccct tgttcctcgt
     5941 gcggaaattg atggagatat cggagatagc catgttggtt tgcaggctcg tatgatgagc
     6001 caggccatgc gtaaacttgg cgcctctatc aataaaacca aaacaattgc catttttatc
     6061 aaccaattgc gtgaaaaagt tggagtgatg tttggaaatc cagaaacaac accgggcgga
     6121 cgtgctttga aattctatgc ttcagtccgc ttggatgttc gtggtaatac acaaattaag
     6181 ggaactggtg accaaaaaga aaccaatgtc ggtaaagaaa ctaagattaa ggttgtaaaa
     6241 aataaggtag ctccaccgtt taaggaagcc gtagttgaaa ttatgtacgg agaaggaatt
     6301 tctaagactg gtgagctttt gaagattgca agcgatttgg atattatcaa aaaagcaggg
     6361 gcttggtatt cttacaaaga tgaaaaaatt gggcaaggtt ctgagaatgc taagaaatac
     6421 ttggcagagc acccagaaat ctttgatgaa attgataagc aagtccgttc taaatttggc
     6481 ttgattgatg gagaagaagt ttcagaacaa gatactgaaa acaaaaaaga tgagccaaag
     6541 aaagaagaag cagtgaatga agaagttacg cttgacttag gcgatgaact tgaaatcgaa
     6601 attgaagaat aagctgttaa agcagtggag aaatccgcta ctttttcgat ttttgattca
     6661 agtttttaga ttatatatag tagcttgaaa taagatatga acaactctat taggaaagtc
     6721 aaattaattt ctagaaatgt tttagcagct acagcgtact attccaaact caaccaacta
     6781 taatagatcg aaactagaat agtacatatc tacttctaaa acattgttaa aaatcgattt
     6841 gactttcctt atttcattcc gctatatata agtttgctgt ttcttgtcgc tcctctggaa
     6901 agctgatata atagctttat gaataaaaaa cgaacagtgg acctgataca tggtccgatt
     6961 cttccctcgc tcttaagctt cacctttcca attttgctat caaatatttt tcaacagctc
     7021 tataacactg ctgatgtctt gattgttgga cgatttcttg gtcaagaatc cttggctgca
     7081 gtaggagcga cgacagcgat ttttgacctg attgtaggtt ttacacttgg tgttggcaat
     7141 ggcatgggga ttgtcattgc tcgttattat ggggctcgga atttcactaa aatcaaggaa
     7201 gcagtagcag ctacttggat tttaggtgct cttttgagca ttctagttat gttgctgggc
     7261 tttcttggct tgtatcctct cttgcaatac ttagatactc ctgcagaaat tcttcctcaa
     7321 tcttatcaat atatttctat gattgtgacc tgtgtaggtg tcagctttgc ttataatctt
     7381 tttgcaggct tgttgcggtc tattggtgac agtctagcag ccctgggatt tctgattttc
     7441 tctgccttgg ttaatgtggt tctggatctc tattttatta cgcaattgca tctgggagtt
     7501 caatccgcag gacttgctac cattatttcg caaggtttat cagcggttct ctgcttttat
     7561 tatattcgta aaagtgtgcc agaactcttg ccacagttta aacatttcaa atgggacaaa
     7621 agcttgtacg cagatctctt ggagcaaggt ttggctatgg gcttgatgag ttcaattgta
     7681 tctatcggca gtgtgatttt acagtcttct gttaatacat ttggtgcagt gattattagt
     7741 gcccagacgg cagctcgacg cattatgacc tttgcccttc ttcctatgac cgctatttct
     7801 gcatcaatga cgacctttgc ttctcagaat ctaggagcta agcaacctga ccgcattgtt
     7861 caaggtcttc gaatcggcag tcgtttaagt atatcctggg cagtttttgt ttgtattttc
     7921 ctcttttttg ccagtccagc cttggtttcc ttcttggcta gttcgacaga tggttacttg
     7981 atagaaaatg gaagtctcta tctgcaaatc agttcagcct tttatcccat tttgagcctc
     8041 ttgttgattt atcgcaattg cttgcagggc ttggggcaga agatccttcc tctagtttct
     8101 agctttattg aactaatcgg aaaaatcgtt tttgtggttt tgattattcc ttgggcagga
     8161 tataagggtg ttatcctttg tgaacctctt atctgggttg ccatgacagt tcaactgtac
     8221 ttctcactat tccgtcatcc cttgataaaa gaaggaaagg caattttggc aaccaaagtg
     8281 caatcctagt tggatttact gaataaaatc catttcctct agtgaaaatc gaaaaaactt
     8341 gtgttctctt ctttagtttg gtgttgaaaa tagtttaaca gacttttgac ttcttttata
     8401 tgatataata aagtatagta tttatgaaaa ggacatatag agactgtaaa aatatacttt
     8461 tgaaaagctt tttagtttgg ggtgttattg tagatagaat gcagaccttg tcagtcctat
     8521 ttacagtgtc aaaatagtgc gttttgaagt tctatctaca agcctaatcg tgactaaaat
     8581 tgtcttcttt gtaaggtaga aataaaggag tttctggttc tggattgtaa aaaatgagtt
     8641 gttttaattg ataaggagta gaatatggaa attaatgtga gtaaattaag aacagatttg
     8701 cctcaagtcg gcgtgcaacc atataggcaa gtacacgcac actcaactgg gaatccgcat
     8761 tcaaccgtac agaatgaagc ggattatcac tggcggaaag acccagaatt aggttttttc
     8821 tcgcacattg ttgggaacgg ttgcatcatg caggtaggac ctgttgataa tggtgcctgg
     8881 gacgttgggg gcggttggaa tgctgagacc tatgcagcgg ttgaactgat tgaaagccat
     8941 tcaactaaag aagagttcat gacggactac cgcctttata tcgaactctt acgcaatcta
     9001 gcagatgaag caggtttgcc gaaaacgctt gatacaggga gtttagctgg aattaaaacg
     9061 cacgagtatt gcacgaataa ccaaccaaac aaccactcag accatgtgga tccataccct
     9121 tacttggcaa aatggggcat tagccgtgag cagtttaagc atgatattga gaacggcttg
     9181 acgattgaaa caggctggca gaagaatgac actggctact ggtacgtaca ctcagacggc
     9241 tcttatccaa aagacaagtt tgagaaaatc aatggcactt ggtactactt tgacagttca
     9301 ggctatatgc ttgcagaccg ctggaggaag cacacagacg gcaactggta ctggttcgac
     9361 aactcaggcg aaatggctac aggctggaag aaaatcgctg ataagtggta ctatttcaac
     9421 gaagaaggtg ccatgaagac aggctgggtc aagtacaagg acacttggta ctacttagac
     9481 gctaaagaag gcgccatggt atcaaatgcc tttatccagt cagcggacgg aacaggctgg
     9541 tactacctca aaccagacgg aacactggca gacaagccag aattcacagt agagccagat
     9601 ggcttgatta cagtaaaata ataatggaat gtctttcaaa tcagaacagc gcatattatt
     9661 aggtcttgaa aaagcttaat agtatgcgtt tttatcctct agtcacttga tttgtttcag
     9721 gtggtttttt agtatagtag aatgaaacga gaacaggaca aattgatcag gacagtcaaa
     9781 tcgatttcta acaatgtttt agaagcagaa gtgtactatt ctagtttcaa tctactatag
     9841 ttaaatctgc ggtcaagtct actggtgaat ctatgattgt aatactcttc caaaatctca
     9901 tcaaccacgt cagtcttgcc ttgcagtctg tatcttactg accaagctag tgatggattt
     9961 agaataggtg atttggagcg tcctattggc taggaaatgc tgctcatagt cctttgctga
    10021 ggctagggtg tttcaacatt caacactcaa ctggttgatc tagttgatag gaagggaatt
    10081 actataaaat actcaggctt ccatcatatt ttttgaaacg attgtgtaat caaaatgtac
    10141 caatattgta gtattggtac agaagatgtt gtgaatggat aaatatatca taactgctgt
    10201 ctcaaaaaga tttcatatgt ctgtgcatat ataatagact tcctgcgaaa ccaaaatcct
    10261 agttcatgat tgataatacc agcactcaaa ttcattcgta atccgaagcg tttacgatga
    10321 tttcgatagg ttgttgaaat cattttaaac gtttttactt tggcaaagat gttctcaacc
    10381 ttgcttctct ccttggatag cgcatggtta caggctttat cttcaactgt tagtggcttg
    10441 agtttgctgg atttacgtgg agtttgtgct tgaggatata tcttcatgag cccttgataa
    10501 ccactgtcag ccaagatttt accagcttgt ccgatatttc cgcgactcat tttgaacaac
    10561 ttcatatcat gacaatagtt cacagtgata tccaaagaaa taattcttcc ttgacttgtg
    10621 acaatcgctt gagccttcat agcgtagcat ttctttttac cagaatcatt cgctaattgt
    10681 ttttttaggg cgattgattt gtacttccgt cgcatcaatc attaccgtgt cctcagaact
    10741 gagaggagtt cttgaaatcg taacaccact ttgaacaaga gttacttcaa cccattggct
    10801 ccgacggatt aagttgcttt cgtgaatacc aaaatcagcc gcaatttgtt cataagtgcg
    10861 gtattctcgc acatattgaa gagtggccat aagaaggtct tctaggctta atttaggttt
    10921 tc
//
LOCUS       DAVLYV010000062        10902 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLYV010000062 DAVLYV010000000
VERSION     DAVLYV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.062"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            130..888
                     /locus_tag="VYO70_001570"
     CDS             130..888
                     /locus_tag="VYO70_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1713778.1"
                     /translation="MALVEFKNVEKYYGDYHALRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLEAVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKTVLENVT
                     LSPIKVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEARAQQFLSKIINHESDKVK"
     gene            928..1692
                     /locus_tag="VYO70_001571"
     CDS             928..1692
                     /locus_tag="VYO70_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1713779.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDTLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1705..2094
                     /locus_tag="VYO70_001572"
     CDS             1705..2094
                     /locus_tag="VYO70_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131131.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1713780.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGVFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSLHPDCPHLLQL"
     gene            2299..3489
                     /locus_tag="VYO70_001573"
     CDS             2299..3489
                     /locus_tag="VYO70_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1713781.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FAGIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKRVHFYYSFRGEENAVYLDLLRDYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(3810..4691)
                     /locus_tag="VYO70_001574"
     CDS             complement(3810..4691)
                     /locus_tag="VYO70_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1713782.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(4832..6160)
                     /gene="vncS"
                     /locus_tag="VYO70_001575"
     CDS             complement(4832..6160)
                     /gene="vncS"
                     /locus_tag="VYO70_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1713783.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVAQD"
     gene            complement(6157..6813)
                     /gene="vncR"
                     /locus_tag="VYO70_001576"
     CDS             complement(6157..6813)
                     /gene="vncR"
                     /locus_tag="VYO70_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1713784.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(6862..6945)
                     /gene="pep27"
                     /locus_tag="VYO70_001577"
     CDS             complement(6862..6945)
                     /gene="pep27"
                     /locus_tag="VYO70_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1713785.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            complement(7125..8504)
                     /gene="vex3"
                     /locus_tag="VYO70_001578"
     CDS             complement(7125..8504)
                     /gene="vex3"
                     /locus_tag="VYO70_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1713786.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(8556..9203)
                     /gene="vex2"
                     /locus_tag="VYO70_001579"
     CDS             complement(8556..9203)
                     /gene="vex2"
                     /locus_tag="VYO70_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1713787.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(9216..10493)
                     /locus_tag="VYO70_001580"
     CDS             complement(9216..10493)
                     /locus_tag="VYO70_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713788.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         3097 a         2400 c         2074 g         3331 t
ORIGIN      
        1 cgatttgact gtcccgatcg atttttcctg ttcttatttc actatagtga atttttcaga
       61 tatggtttga caagaacatt cttttattgt agaatcgtgt tataaaacct aacataaagg
      121 agatctctca tggctttagt agaatttaaa aacgtcgaaa aatattacgg agactaccac
      181 gcactccgca acatcaatct ccgttttgaa aaaggacaag ttgttgtcct gcttggacct
      241 tctggctctg ggaagtccac tcttatccgt acgatcaatg gtttagaggc tgttgacaaa
      301 ggaagtctcc tagtcaatgg gcaccaagtt gctggtgcca gccagaaaga tttggtacct
      361 cttcgcaagg aagtcggcat ggtttttcaa cattttaacc tttatccaca caaaacggtg
      421 ttagaaaatg tgacactttc gcccattaaa gttctaggaa ttgataaaaa agaagctgaa
      481 aaaaccgccc aaaaatatct ggaatttgta aatatgtggg acaagaaaga ttcctatccc
      541 gccatgctat ctggtggaca aaaacagcgg atcgccatcg ctcgtggtct tgctatgcat
      601 ccggaactcc tcctctttga tgaaccaaca tctgctcttg atcctgagac tatcggagat
      661 gttctagcag ttatgcagaa actggcgcat gatgggatga acatgatcat cgttacccac
      721 gaaatgggct ttgctcgaga ggttgcggac cgcattatct ttatggccga cggagaagtt
      781 ttagtagata cgacagatgt cgataacttt tttgacaatc caagcgaagc tcgtgcccaa
      841 caattcctca gcaaaattat caaccacgaa agtgacaaag tcaaataagg aggcgcctat
      901 gaaaaaaaat tctttttatc agcattattg attagccttt tcggccttgc tgctgccaaa
      961 ccagtccagg ctgatactag tatcgcagac attcaaaaaa gaggcgaact ggttgtcggt
     1021 gtcaaacaag acgttcccaa ttttggttac aaagatccca agaccggtac ttattctggt
     1081 atcgaaaccg acttggccaa gatggtagct gatgaactca aggtcaagat tcgctatgtg
     1141 ccggttacag cacaaacccg cggccccctt ctagacaatg aacaggtcga tatggatatc
     1201 gcgaccttta ccatcacgga cgaacgcaaa aaactctaca actttaccag tccctactac
     1261 acagacgctt ctggattttt ggtcaataaa tctgccaaaa tcaaaaagat tgaggaccta
     1321 aacggcaaaa ccatcggagt cgcccaaggt tctatcaccc aacgcctgat tactgaactg
     1381 ggtaaaaaga aaggtctgaa gtttaaattc gtcgaacttg gttcctaccc agaattgatt
     1441 acttccctgc acgctcatcg tatcgatacc ttttccgttg accgctctat tctatctggc
     1501 tacactagta aacggacagc actactagat gatagtttca agccatctga ctacggtatt
     1561 gttaccaaga aatcaaatac agagctcaac gactatcttg ataccttggt cactaaatgg
     1621 agcaaggatg gtagtttgca gaaactttat gaccgttaca agctcaaacc atctagtcat
     1681 accgcagatt aaggaggata ccctatgaca gatttatcat cttggacagc ctattttcag
     1741 gattttggac aatttttcaa tggtttcctc tttactcttg ccctagcgat tggatccttt
     1801 atcctcgcca tggtcttagg agtcttcttt ggtgccatgt caaccagtaa acgtccaatt
     1861 ttacgcattt tagctcgcat ctttgtggaa ttttaccaaa acactcccct cttggtgcag
     1921 tttgttattg ttttttatgg cctacctctt atcagtgacc atactatcat gattccgatt
     1981 tattggacag ctgttctctg cgtgggactc tatcacggcg cttatatcgc tgaggttatt
     2041 cgttcaggga ttcagtcttt acatcctgat tgccctcatc tacttcagct ttaacctagc
     2101 aatttctaac ctgtctcata tgctagcaaa acgttggcaa caagctgcag aataacaaat
     2161 tagctctcta gttcattgga gagttttttg atgatagtag atatttatag acctcaattt
     2221 gacaccatac attttctatg ataaaatgta acaaattcat ttacaacaaa tcgaaatcta
     2281 aatgaaatgg aattttctat gaaatcagtc aaaggactac tctttatcat agctagtttt
     2341 atcttgactc ttttgacttg gatgaacact tctccccaat tcatgattcc aggactagct
     2401 ttaacaagcc tatctctgac ttttatccta gccactcgtc tcccactact agaaagctgg
     2461 tttcacggtt tggagaaggt ctacaccgtc cacaaattca cagcctttct ctccatcatc
     2521 ctactaatct ttcataactt tagtatgggc ggtttgtggg gctctcgctt agctgctcag
     2581 tttggtaatc ttgccatcta tatctttgcc ggcatcatcc ttgtcgccta tttaggcaaa
     2641 tacatccaat acgaagcttg gcgatggatt caccgcctgg tttacctagc ctatatttta
     2701 ggactctttc acatctacat gataatgggc aatcgtctcc ttacatttaa tcttctaagt
     2761 tttcttgttg gtagctatgc ccttttaggc ttactagctg gtttttatat catttttcta
     2821 tatcaaaaga tttccttccc ctatctaggg aaaattaccc atctcaaacg cttaaatcac
     2881 gatactagag aaattcaaat ccatcttagc agacctttca actatcaatc aggacaattt
     2941 gcctttctaa agattttcca agaaggcttt gaaagtgctc cacatccctt ttctatctca
     3001 ggaggtcatg gtcaaactct ttactttact gttaaaaatt caggcgacca taccaagaat
     3061 atctatgata atcttcaagc cggcagcaaa gtaaccctag acagagctta cggacacatg
     3121 atcatagaag aaggacgaga aaatcaggtt tggattgctg gaggtattgg gatcaccccc
     3181 ttcatctctt acatccgtga acatcctatt ttagataaac gggttcactt ctactatagc
     3241 ttccgtggag aagaaaatgc agtctacctt gatttactcc gtgactatgc tcagaaaaat
     3301 cctaattttg aactccatct aatcgacagt acaaaagacg gctatcttaa ttttgaacaa
     3361 aaagaagtgc ccgaacatgc aaccgtctat atgtgtggtc ctatttctat gatgaaggca
     3421 cttgccaaac agattaagaa acaaaatcca aaaacggagc ttatttacga aggattcaag
     3481 ttcaaataag taatcatact ctcctggaat ttcccaggag agttttttat agccaaatga
     3541 aataagaccg gaacgatgga attttggcat tcaaatcaat tgataagaat attttatagt
     3601 ggagtgaaat aggatacgaa caaattgatt aggaaaatca aatgaattta tagaaatctt
     3661 ttagcagtta tgttatccta ttctagtttc aaaacgctat agaagcagca ttgtgctagt
     3721 ctagattcag tttactatac taaaacgagt agcttgaaat caaaaaaccc accctcacag
     3781 gcaggtttta tctgtattat tcagctagat tatgctttac cttctgaacc gaatacgtcg
     3841 atacgttctt caaccgatgc ttggatagct tttacaccgt cagccaagaa tttacgtggg
     3901 tcgaagagtt ttttcttgtc gtattctgct tcgtttgctt cgtagtcacg agcaaattta
     3961 cgagttgcgt tagcgaatgc gatttggcat tctgtgttaa cgttaacttt ggcaacacca
     4021 agtttgatag ctgcttggat ttgctcatca ggaatacctg atccaccgtg caatacgatt
     4081 gggaatcctg gaagagcttc tgtcaatttt tgcaagtggt caaggtcaag accttcccag
     4141 tttactgggt aaggaccgtg gatgttaccg ataccagctg ccaagaagtc gataccagtt
     4201 tcaaccattg ctttagcgtc ttcgattgga gccaattcac ctttaccgat gattccgtct
     4261 tcttcaccac cgatagtacc aacttcagct tctactgaga tacctttagc gtgtgctttt
     4321 tcaacaactt ctttagccaa tttaagattt tcttcaactg gaaggtgtga accgtcaaac
     4381 atgattgaag tataaccaac ttcgatacac tcaagtgcat cttcgtagtg accgtggtca
     4441 aggtggatag ctactggtac agtgataccc attgattcaa caaggttagc gatcaagttg
     4501 cgagcaactt tgtaaccacc catgtattta gcagcaccca ttgaagtttg gatcaaaact
     4561 ggagcttttt tagcttctgc tgcgcgcaag atagcttgag tccactcaag gttgtttgtg
     4621 ttaaatccac caactgcata accgttgtca cgggctgctt ggacaaattt ttctgctgaa
     4681 acgattgcca ttttatcagg cctcctgtat atttttatgg gtcatcccat ttacattgtt
     4741 cattttatca ctttttgcca aaaaaatcta gtttttcccg cagtttcgat tgattttctt
     4801 ctaactccat ctatgtaaac cctttctctc cctagtcttg ggcgactttt ggaaaatcta
     4861 taaagaaggt taaacgattc tcctccatct cgaaacgata agctaatttt tcatgttcta
     4921 atagactctt aaccacaaag agccccatac cagacccctt gaccttgcga ctagcattgt
     4981 cagaaaaaga ctgggctagt ttttcttgtt cctctgagct acagctattt tcgataaaaa
     5041 gttctccttc tctttctcca attcgaacta agccacctgg aacagagtgc ttaatggcat
     5101 tgctgatgag attagaaaga atcaacttca taactgatgg gtttagataa gcctgctgat
     5161 gggtcaaact attgtctatc tggagctctc tttccttggc tagcaaggca taatctttga
     5221 ccagattttg cgtcatctgg aggaggtcaa ttgtttccct atcatctcgc aattcctgca
     5281 cagaagagag ggaaagtatc tgcagaacat ggtgattgag ttcatccaca atccccaagg
     5341 caactcccag atactggtct ctatccttat aacgaccgat attctctctc atattttcga
     5401 ttaggatttt caaactagcc agcggtgttt tcaattcatg agaagctcct cgtaggaatt
     5461 cgaccttcat cttctccagc tggagaatgg cttcattctt ttcatgcaag tccgcaataa
     5521 cagtcaagag atgctggtag aggctattga tttgttcctt gagattacct atctcatcct
     5581 tagaatccac gcgcaatcgc acttgggaat ccaggtccat catccgacgg gtcacccgct
     5641 tgatttccaa aatcggtgca acaatagtcc gagcgtagat gtaggccacc aagagggaaa
     5701 tcagaaagga ggccagcaag gtatagggaa gaaactggag actgatttgc tccgcttcct
     5761 tttgtaaatc catggaagct agaaactgga gaatcatagt accaccgtct tgcgttttca
     5821 cctcgcgctc ctcaataaag agagaggttg tctggcggtc tgtgtccaga ggaagactgt
     5881 ccttgacttc taacttgtcc tcggtcatct cacctttgac ggtccccttg atatcactag
     5941 tctgggaata caaatctaac acttgctcga tactctgcct atctttccct tctagggact
     6001 gggcaatggc tgttgccttt tgaccaatgg tttcctgacg atgactcaga taagtcgaag
     6061 gaaaaagaaa ataaatagct aaatgaaggc agataaccag aacactaaat atcgagaagg
     6121 tatagataaa tatctttgca aataaacctg ttcgtttcat tttcgctcca atttataacc
     6181 aacattgcgc acagtgagga tacaatccaa gtctagcttt ttccgcaatt ccttgatata
     6241 aacatcaata acacggtcaa agggaacctc atctgtcgct ttccagacgg catcgataat
     6301 ctgagaccga gtcaaggccc ggccttcatt tttcactaga tagtccagaa tttccaactc
     6361 tttggcattg ataggcactt cttgacctgc gaggcttgca ctgtagcttt caaagtccac
     6421 cttggtatcc ttgtaagaaa agattcgtcc tgtatcgtag tagcgcttga aaatcgcgtc
     6481 caccctcact tttaagaggg agagggagaa aggtttttcc agatagccat ctgccaaaga
     6541 ggcaaaggca ctcatcttgt attcctcatc ctgaaaagct gtcaacatca agacaggaac
     6601 ctgactggtt ttacgaatct cagctagaac ttctaagccg ttaagcttgg gcatctggat
     6661 atccagtaaa accagggcca cctcatagct agaaaattgc tccagagctt cctgaccgtc
     6721 cgctgcctca atagtttcat agccacaatc cgtcaaataa tcactgaccc cctcacggat
     6781 catctcttca tcttctacaa ttaaaatttt catactttaa ctgctctcta ttttttattt
     6841 ttcttagaat aaatacctac tctattttct attatagtct cttgctggcc ttttgtctgt
     6901 aagtaactga tcactagata aaacgttgtg aaattccttt ctcataaatt ccatagcttt
     6961 agtatattat atttaagcaa taaagtacaa agaaaacaac tgaaagcaat gattttcacc
     7021 actgctttcg gatttatttt gaattgttaa atagccattc ctatccacta ttcttgaata
     7081 gaaacacaag atgcaatctt tattccagac tcattttttc aaatttattc accatccagc
     7141 aagagctctt ttggttgttt tctaaggaga ttgcttgaag caagcgccat aacgagaacc
     7201 actagaacca aggcaaggac aaaaatgatg ataaagtctg atgtctgaat agaaatgtct
     7261 aggctcgaca aggtcttgct aaagccatct acttctgcac caccaccaag gttagaggct
     7321 tgagccgcct tactagcctg tttggcaaca cctgaagtca cattggcaag gacagtgttt
     7381 ccaattgcac gggcagtgta attagctagg aagtaagcag aaactagagc agggatagca
     7441 atcaagatag attcggtgat gaattgaccc aagatacttg cctgcttgag gccgatagag
     7501 aggagaattc ccacttcctt gcgacgggcg ttgatccaaa ggctgagcaa gagggcaagg
     7561 aggagaactg agaagctcaa gctaccccag aagaggaggt tggccatctt gtacatacca
     7621 gatatagatt gctcaagagc tgggtagtta gaggagctct tgacgagtgt gtagctcttc
     7681 cagttgatac cactgatgcc attcaactct ttcataacat catccaagtt cttgtctgct
     7741 gttacaaaga aggttgcgtc cccataaatg gctgtgtctt ctgtgtatcc ataaagtttt
     7801 gcagcagtgt gaatgtctgt aatagctgtg ttttcgtaaa gttcttgtga gtaggttact
     7861 gctgacttat tatgaccatc aaagagtccc ttgattgtca cttcaactgt ttccttggct
     7921 cctttttcat tatctgcatc gtagatatta gagtccagtt taaccttgtc ccctactttc
     7981 cagccgtgtt tggctgccaa gtccttgtgc aagaggattt tatccttgtc gtcgttggtt
     8041 aagtgctctc cttcgactag tttataagaa ccagagacaa acttgtcttc tttagaggag
     8101 tcattgacac ctgtaatcat caagctactt ccaaaacgtt tggcacgatc agcagtgaga
     8161 ttcttcttgg tttctggcgt ttcaatcagg tcatatccag tcaaatctcc gatagcgttg
     8221 atacgtttga cataagactc aatggccttg ttttcggtga tttttttgat gtcttcaccc
     8281 ttgatattcc cagcaccgcg aggcgttcct tggttgacgc gacgattgat ttgcatggag
     8341 aagctattgg tgatattttt aaaggtctcc tgagaagcct tggcagtagc tcccttgatt
     8401 gacaagccga ccaaactcaa gctcgccatg aggagaataa tcaggaagat gacaatcgat
     8461 ttgaaaaact tccttgtaac ataggcaaat gcgttgtgta acatagattc cctttctaga
     8521 ttttgtttta atcattctat caaaataagc tcaaattatt tactagtatt gcgcgtttca
     8581 gtcagtttct tatcctttaa ttcaagtgta atatctgacg cttgtgccac ttctttactg
     8641 tgagttacga caatcacaca tttacctgtt ttctgggcaa gtgatttgag tagttcgaca
     8701 atatctccag cagttttagg atccagattt cctgttggct catcagctag aataactgga
     8761 gcttctgaga ccaaactgcg agcaatggca acacgttgct gttgaccacc tgataactgg
     8821 agaacattcc gcttgatctg gctttcatcc aaaccaagct caagaagtgt attcttgctt
     8881 gcctttttgt tgaccaatcg gatattttcc agcggagaaa gataatctat caagttataa
     8941 ttttgaaaga ccagggaaat atggtgcatg cgatggtaag aatagccctt cttacgaata
     9001 tcctctcctt gaaaaaggat agaaccttca acaggactat ctagaccagc aagtagggac
     9061 aagagtgtgg attttcctgc tcctgactcc ccaataatac tgtaaaattt tccgggttca
     9121 aaattataat tgatctgata taggactgct tcagcagtat tcttataacg gtaggtaaca
     9181 tcttgtaatt gtaataaagt catgatttct ccttcttaac taatagatga taaaatttct
     9241 ttcggtgatt ttctaaataa gaataggaaa caaagggcta cagataagca actaagcaga
     9301 actagaaaaa cataggattc tgcaaaagat aagatactag ttgataaact gcttgctttg
     9361 gctagtgtat cttgtaagct tgcctgatct ccacttgcta gtagagtttg gagtaggtaa
     9421 gttgtgattc cgtttcctgc aacaaatgct ggaagcaaag ctccaagaga taccaaaact
     9481 acctctaaac agaattgtag gaagatcgag ctcttgcctt ttccaagtgc aagtaaaatc
     9541 cccacttcat agacccgttc tctcaaccag agagacaaaa ccagaattaa ggctccagct
     9601 cctgctatca acatcccata aaggaagatg gtcaggaagg tttggaaagt tgcaactgag
     9661 tctttgattt gttcaaaagc cttgttttcc ttttcgactt ggtagccttg attttccaag
     9721 gccaagtttt ctacctgctt catgagtccg tccatttcct taggattttc tacatagaag
     9781 cgtgctgcac tgacttgagc ttcactattg cccaaaaggg tttggctact ttcatagtct
     9841 gtaaagactt gattttcact gaagtcagaa gacaagcctg tgaatttctc ttgtttttta
     9901 ccagaaaaga tgccaataat ctcaaactct actgtttgtc cttttccaga ttcagactga
     9961 ccagcatcca agccaatctt gtcatgaagt gaaagaccgt tcttcttagc caattcttcg
    10021 tggataagga ttttcttgga atcccctttt tgaaggtgtc gcccttcttt tagattgaaa
    10081 gccgaactgg taaaggttac atccttggat gaatcctcaa gagccgttaa gctaaccaag
    10141 ttattgtctg cagctgataa atcatcacgc tccacgctct gctcgccagt cactgcttcc
    10201 ttgtctttta gttttgcgac cgtctcaagt tcaggagaga cattttccag ccccttaatc
    10261 ttgcttacag atgctaggtc tgacaacttg aatgtctgac cattctctat cttcttaata
    10321 gaaaaagatg tattgagtga tttataaaga ttgctttcta ctgttttgtt ggacttcatc
    10381 agagtcaaac aggctgaaat tccggccaat aagaccaata aaatcagaaa taaaataaaa
    10441 cttctcagtc gctttctgct gacataagcc caagatcttt ggattggatt catttgtcac
    10501 ctccatattt gtaagactat tataaaaccc aaatatgaaa tgtttatgaa atacgaaaaa
    10561 aaatatcgag taggggataa tctctcgccc ctctcacacc accgtacgtg ccgttcggca
    10621 tacggcggtt caactaactt ttaacgcatg tcgttcaagg taataatcca aacacgaaac
    10681 ctgttcacgt ttttcaagga ctggttttga tatagcacgt ttaagtaccg acttctgagc
    10741 tactatagta gattgaaact agaatagtac atctcttctt ctaaaacatt gttagaaatc
    10801 gatttgactt ccctgatcga ttcgtcctat tcttatttca ttttaatata attgatagtg
    10861 gtcgccccag ccagatacct tatctgctat ccatttagga ac
//
LOCUS       DAVLYV010000063        10821 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLYV010000063 DAVLYV010000000
VERSION     DAVLYV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10821
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.063"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(36..842)
                     /gene="yidA"
                     /locus_tag="VYO70_001581"
     CDS             complement(36..842)
                     /gene="yidA"
                     /locus_tag="VYO70_001581"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1713789.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLTKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(855..2159)
                     /locus_tag="VYO70_001582"
     CDS             complement(855..2159)
                     /locus_tag="VYO70_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1713790.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLAE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            2231..2608
                     /locus_tag="VYO70_001583"
     CDS             2231..2608
                     /locus_tag="VYO70_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1713791.1"
                     /translation="MKIRMRNTIQFDEQLEVIDRLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            2699..3031
                     /locus_tag="VYO70_001584"
     CDS             2699..3031
                     /locus_tag="VYO70_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1713792.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            3043..4614
                     /gene="ffh"
                     /locus_tag="VYO70_001585"
     CDS             3043..4614
                     /gene="ffh"
                     /locus_tag="VYO70_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1713793.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVCHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(4809..6092)
                     /locus_tag="VYO70_001586"
     CDS             complement(4809..6092)
                     /locus_tag="VYO70_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1713794.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(6346..7059)
                     /gene="rsmG"
                     /locus_tag="VYO70_001587"
     CDS             complement(6346..7059)
                     /gene="rsmG"
                     /locus_tag="VYO70_001587"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1713795.1"
                     /translation="MKPETFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            7153..7713
                     /locus_tag="VYO70_001588"
     CDS             7153..7713
                     /locus_tag="VYO70_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1713796.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            7715..8614
                     /gene="htpX"
                     /locus_tag="VYO70_001589"
     CDS             7715..8614
                     /gene="htpX"
                     /locus_tag="VYO70_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1713797.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(8658..10217)
                     /locus_tag="VYO70_001590"
     CDS             complement(8658..10217)
                     /locus_tag="VYO70_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1713798.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRLLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(10456..10773)
                     /locus_tag="VYO70_001591"
     CDS             complement(10456..10773)
                     /locus_tag="VYO70_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1713799.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EQLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYDHVKHVLSLYGPV
                     FEF"
BASE COUNT         3119 a         2022 c         2446 g         3234 t
ORIGIN      
        1 tattgctaat caattcttat tgaaaaatga tacttttaca gtacccatgt tcggatggcg
       61 tgggcaacgc cggattcgtc atttgtttta gtgatgtatt tggcgatttt tttgatttct
      121 ggatttccat tttccatgac aacggggttt ccaacgactt ccagcatggc acggtcattt
      181 tcttcgtcac cgattgccat ggtttcatct ttggtcaatc cgagtttttc agccaagtga
      241 gtaatggctg aacccttgtc tacattcttt gtaaggagtt cgaggtagaa aggagcagat
      301 ttgttgatgg agtagcgctc gtaaaattct gctggaattt tttcaatcgc agcatcgaga
      361 atttctggtt catcgataaa catacattta acaatttctt tgccagccat ttcttcaggg
      421 gtacggtaga agataggcat gctgacgagg gttgattcgt gtacagtgta ttttccgata
      481 ttgcgatttg cagtatagat accgtccttg gtaatggcat gcatgtggac accgagcttg
      541 cgactgagga attccatatc cagataatcc tcataggtca aggattcgct gataatctca
      601 tggccagtag cagtttcttg gacaagggca ccattgaagg tcaccacata gtcaccttgg
      661 tctctcaact gcaagtcgtc cagaagtttg gcaacacctg cgatggggcg gccagttgca
      721 atcacgactt tgacaccagc ttctttggca tcttggatgg cagaaaagac ttcaggagtg
      781 atttcctttt ggctgttgac tagggttccg tcaatatcga cggcaattag tttaatactc
      841 ataaatttcc tcttctagtt tttatttggg gtaaaatgat cgttctcaat caagtgtaaa
      901 aattgctggg taatgcttgc gaagatgctg ttttggtcta acatttcttt agggaaatag
      961 aaacgattat ctccgtggcg actgccagca agggattgga cgataagaga caggctagag
     1021 agttcggcca gttctccatt tttttgtaaa atctcaatct gtgtccgtgg gttttcagat
     1081 tcgggacgat agatatcata agggaggtca aagttcttat gaatggcagt gtagtagtcg
     1141 ggatcaaagc cgatatcctc agctaatttt ctcatgctgg cgagttggtc ttggtcctct
     1201 tgtgaaaagg taatggattt aaagaccttg cggttgacaa agcgttgtga caagtctgcg
     1261 agaatcttgt caggactggt catccagagt tgaaagtagg tattcatcac accatcatct
     1321 agagccagat agtcagtcaa ggtcacattt ttttcaaaga aaggcaggag gtgtggagaa
     1381 gttcgtgcaa agaagtcctt gtcttcagga tagagttcct tggcgcgttt gagaagattc
     1441 tgtaggagaa cttccatggc gcgtgttgcg gggtggaaat aaacctgcat gtacatctgg
     1501 tagcgactga ggacgtagtc ttcgatggcg tgcatgccat tgcgctgaaa ggcgatacca
     1561 ttttcgatag gacgaatgac tcggaggatt cgagtcaggt caaattcccc ataggatgct
     1621 cctgtaaaat aggagtcacg caagagatag tccatgcggt ccgcatcaat ctggctagaa
     1681 atgagctgca cgacctgctt attaggatag gtatggtcaa tgacactggc caccttttct
     1741 gggaaatcag gtgccacttg tagcaggact tggtgaatct ctgtctcagg attttgaata
     1801 atctcctgag taatggcttc atggtctgta tcaaagagat tttcaaaagt atgggagtag
     1861 gcaccatgcc caaggtcgtg taggagagca gcggtcatgg tcaagagaga ctcggcagga
     1921 ttccattcct caggatattt ttcttcgaaa atctctgtga tgcgtcgtgc aatttcatag
     1981 actcctagac agtgagagaa gcggctgtgc tctccaccgt ggaaggtata actggaggtt
     2041 cccagttgct tgatacggcg caaacgctga aattcttttg tattaatcaa gtcatagata
     2101 atttgattat tgacatggat gtagttgtga actgggtcac ggaatacttt ttcgttcata
     2161 tgaccattat agcaaaatcc tgctcttttt ggtaaaatag taggacaaga gacaagaaag
     2221 agggcctgat gtgaagattc ggatgcgaaa tacaattcag tttgatgagc agttggaagt
     2281 gattgaccgg ctttatgatg tggaagtgca tgaaaaagga gattatagct acctgctttt
     2341 ctataatgag gaaaaggaaa aagtagtgat taaatttcat ggtcaagaac ttgtgatgag
     2401 ccgattttct aatcccaaga ccattatgcg ctttctaaag gatagcgata gtttagccta
     2461 tattcctaca cctatgggca tgcaggagtt tatcatccaa acgagccatt atcaagttga
     2521 taggcaaaag attgaactag attatcaact acaaaatcaa gagggacatc cttttgccag
     2581 ctatcaattg gaaattactt ggggctaggc tcatgtcttt ttcaaattta gctatctatc
     2641 ttcttggatc ggcttcctag tgtggtagat taggctaatt tttggtataa taaaagttat
     2701 ggaaatcgaa aaaaccaatc gtatgaatgc gctctttgaa ttttatgcgg cgcttttgac
     2761 agataagcaa atgaattata tcgagctcta ctacgctgat gattacagcc ttgctgaaat
     2821 tgccgaggag tttggtgtca gtcgtcaggc tgtctatgac aatatcaagc gaacagaaaa
     2881 gattctggaa gattatgaga tgaaattgca catgtactcg gactatattg tccgcagtca
     2941 gatttttgat cagattttgg agcgctatcc caaggatgac tttctgcagg agcagataga
     3001 aattttaaca agcattgata atagagaata agaggaagaa aaatggcatt tgaaagttta
     3061 acagaacgtt tgcagaacgt ctttaaaaat ctacgtaaaa aaggaaaaat ctctgaatct
     3121 gatgtccaag aggcaaccaa agaaattcgc ttggccttgc tcgaggccga cgttgccttg
     3181 cctgttgtaa aggactttat caagaaagtt cgcgagcgtg cagtcgggca tgaggtcatt
     3241 gatacactta atcctgcgca acagattatt aaaatcgttg atgaggaact gacagccgtt
     3301 ttaggttctg atacggcaga aattatcaag tcacctaaga ttccaaccat catcatgatg
     3361 gttggtttgc agggagctgg taaaacaact tttgcaggta agctggctaa caaactcaag
     3421 aaggaagaaa atgcccgtcc tttgatgatt gcggcggata tttaccgtcc tgctgccatt
     3481 gatcagctga agacactggg acaacagatt gatgtgccgg ttttcgctct tgggacagag
     3541 gttccagctg ttgagattgt acgtcaaggt ttggagcaag ctcaaactaa tcataacgac
     3601 tatgtcttga ttgatactgc gggtcgtttg cagattgatg agctcctcat gaatgagctt
     3661 cgtgatgtga aagcattggc tcaaccaaat gaaatcttgc ttgtcgttga tgctatgatt
     3721 ggtcaggaag cagccaatgt tgcgcgtgag tttaatgctc agttggaagt gactggggtc
     3781 atccttacca agattgatgg cgatactcgt ggtggtgctg ctctgtctgt ttgtcacatt
     3841 actggaaaac caatcaagtt cactggtaca ggtgaaaaga ttacggacat tgaaaccttc
     3901 cacccagacc gcatgtctag ccgtatcctt ggcatggggg atatgctcac tttgattgag
     3961 aaagcttctc aggaatacga tgaacaaaaa gcccttgaaa tggctgagaa gatgcgcgaa
     4021 aacacctttg attttaatga tttcatcgat caattagatc aggtgcaaaa tatggggccg
     4081 atggaagact tgctcaagat gattccaggt atggccaaca atccagcact tcaaaacatg
     4141 aaggtggatg aacgccagat tgctcgtaaa cgtgcgattg tgtcttcgat gacacctgaa
     4201 gaacgtgaaa acccagattt gttaaatcca agccgtcgcc gtcgtattgc tgctggttct
     4261 ggaaatacat tcgtcgaagt caataaattc atcaaggact ttaaccaggc taaacagctc
     4321 atgcagggtg ttatgtctgg ggatatgaat aaaatgatga agcaaatggg gattaatcca
     4381 aataaccttc ctaaaaatat gccaaatatg ggaggaatgg atatgtctgc ccttgaagga
     4441 atgatgggac aaggcggtat gcctgactta tcagctctcg gaggagcagg aatgccagat
     4501 atgagccaga tgtttggtgg cggtttgaaa ggtaaaattg gtgaatttgc catgaaacag
     4561 tccatgaaac gtatggctaa caaaatgaag aaagcgaaga agaaacgcaa gtaagacaag
     4621 agattgatgc aaatctcatt tgatgagaaa ataaagtaaa aatttctatg ataaaacgta
     4681 cagtatcaag gttttagaat atctgatatt atacgttttt tgattttaat aattttgtta
     4741 agacactttt attatggtat acgaaaaatc cactcagctg tctgagtgga ttaggtgtat
     4801 ttagactctt agtctttatt ttcgtatggc aagatcaagt tcaagatgat tcctgtcatg
     4861 gctgaaaggg cagtacctga aagtgtaact ggaccaagtt taaggatagc tcctccaagt
     4921 ccaagaacca acatagcact tgcgatgatg aggtttcgca tttgagcgaa atcaacacgt
     4981 tctttaatca agactttcaa accattgctg gcgataaccc catagagaag gattgacata
     5041 ccaccaagta cagcgtttgg aatagttgaa atcaaggcag tgaatttacc aaggaagctg
     5101 agggcaatcg cgatgaaggc agcgttacgg ataactgaga cagaagcgat acgagtcata
     5161 ccgataaccc ctgtattttc tccgtaagtt gtattggctg gtccaccaag gaaggcagaa
     5221 acagaagttg cgataccgtc accaagaaga gtacggtgaa gacctggttc ttttaagaat
     5281 tgacgaccac agatttgacc caaaacagta tggtctccga tatgttcaga aattgttacg
     5341 atagcgattg gcaagatagc gatggcttct ggaccaaagt aaagattgta ctctttaaag
     5401 gcaccacctg tgctaaatgg caagtagaaa ccaggaattt caaaccagtt agcttctagg
     5461 acaggtgtaa agtcaaccaa gccaagagtt agtgcgaaaa ggtaaccacc gataatggca
     5521 aagaggaatg gaatgattcg taggaagcct tttccttttg tattgataaa ggcagcaatt
     5581 aggaaagtaa caacggctac cagagcattt ttccaatttc cgtctgctac aagacctgca
     5641 ttggtaacag ctgaacctgc aagtccaaga ccgataacga tgatcatagg accgataatg
     5701 attggtggca agagtttatc aatccatttt gttcctacaa atcggatgct ggtagcaaca
     5761 aggacataga ccaaaccagt caagataacc cctgtttggg cagcagatac atcccccccc
     5821 atttctttca tagccagtga catagctgtg ataaaggcaa atgaagaacc tagataaact
     5881 ggaactttaa aaccagtagc aatcatgtag atgagtgttc caacacctga agcaaaaagg
     5941 gcaacagata caggcattcc caaaatcaat ggtaccaaga tggtcgcacc aaacatggcg
     6001 aaaacgtgtt ggaaactaag gagaattcct tttccatccg aaggacgttg gtcaacgtct
     6061 agtaacaaat caacagttga ttcttgtttc atataaacct cacttttggg catcagaaaa
     6121 gagcccatta ttttacagca ataggccctc agagtaagac ttctctaaaa tctagacttt
     6181 aaagagtttc aaatatttct gcgtcttcgt cacctcacgg gatgacatta aaaacctttg
     6241 cttaggatag tatagcagaa aaaaatgatt ttgtaaatca ttttttcccg agcctaaaaa
     6301 taagagagcg aggttgattt tgtaaatatt tttttactta aaaatttaaa gcgggcgttt
     6361 atttggcata ccagccttac gtggatattt atttggtgtt tcttttttct tttctaccac
     6421 tgtgatgtaa cgcggatctc tatttggtag ggcgtagctg agattgtctt cgactttact
     6481 aaaaaggaga ttgagggcat tcttagcttc taacaactcc tcaggcgcat tgctggcctt
     6541 gagtgccaat agttttccac caaccttaag gtagggaata gtcaattcag ataggacctg
     6601 catacgggca accgcacgag ctgttacaaa atcatattga gcacggaagt tcttgtcttg
     6661 ggcaaaatct tcggcacgtc cgtggtagaa atgaactccg ttcaaatcca gttcttgagc
     6721 caagagttgt aggaagttga tgcgcttatt gagtgaatca ataatggtca catctaactc
     6781 cggatagaga attttcattg gtagactagg aaatcctgcc ccagccccga tatcaagaag
     6841 tttgatagtt tcattgggaa tcaaaccttg aagaatgggt gcaatcgaat cgtaaaaatg
     6901 tttgagataa acttcttcct tgtccgtaat cgccgtcaaa ttaatcttct cattccactc
     6961 gaccaagagc tcaaaataac gttcaaattg ttctttttgc tggtccgaaa gtggaagatt
     7021 ctgctcggca agcaagttgt aaaatgtttc tggtttcata gttatttcct tctttagtat
     7081 catcttattt taccacaatc attaaaaatt tgatatactg gtactaatct aaaagtagaa
     7141 aggacttata tcatgacttg gattattctt ggagttatcg ctcttattgt tatttttgtg
     7201 attgttagct ataacggttt ggttaaaaat cgtatgcaaa ccaaggaggc ttggagtcag
     7261 attgatgttc agttgaaacg tcgaaatgac ctcttgccaa acttgattga gactgtaaaa
     7321 ggttatgcca aatatgaagg ttctaccctt gaaaaggtgg cagaactacg taaccaagtg
     7381 gcggcagcga cttcaccagc agaagctatg aaagccagtg atgccctcac tcgtcaagtt
     7441 tcaggtattt ttgcagttgc agaaagctat ccagatttga aagctagtgc taactttgtt
     7501 aaattgcaag aggagttgac aaacacagaa aataaaattt cttactctcg tcaactctat
     7561 aacagtgttg tcagcaacta caatgtaaaa ttagaaactt tcccgagcaa tattatcgct
     7621 ggaatgtttg gatttaaagc ggcagatttc cttcaaacac ctgaagagga aaagtcggtt
     7681 cctaaagttg attttagcgg tttaggtgac taagatgttg tttgatcaaa ttgcaagcaa
     7741 taaacgaaaa acctggattt tgttgctggt atttttccta ctcttagctc ttgttggtta
     7801 tgcggttggt tatctcttta taagatctgg acttggtggt ttggttattg cactgattat
     7861 cggctttatc tacgctttgt ctatgatttt tcaatcgaca gagattgtca tgtccatgaa
     7921 tggagcgcgt gaggtggatg agcaaacggc accagacctc taccatgtag tggaagatat
     7981 ggctctggtc gctcagattc ctatgccccg tgttttcatc attgatgatc cagccttaaa
     8041 tgcctttgcg acaggttcta atcctcaaaa tgcggctgtt gctgcgactt caggtctact
     8101 agctatcatg aatcgtgaag aactagaagc tgttatggga catgaagtca gtcatattcg
     8161 taattatgat atccgtattt cgactattgc agttgccctt gctagtgcta tcaccatgct
     8221 ttctagtatg gcaggtcgta tgatgtggtg gggtggagca ggtcgcagac gaagtgatga
     8281 tgaccgagat ggaaatggtc ttgaaatcat tatgctagtg gtttccctac tagctattgt
     8341 actggcacct ctcgctgcaa ccttggttca gctcgctatt tctcgtcaga gggaatttct
     8401 ggcagatgca tctagtgtcg agctgactcg caatccccag ggaatgatta atgccctaga
     8461 taagttggac aatagcaaac ctatgagtcg ccacgtcgat gatgctagca gtgcccttta
     8521 tatcaatgat cctaagaaag gtggggggtt ccaaaaactc ttttataccc acccacctat
     8581 ctcagaacgg attgaacgtt taaaacagat gtaaaagaat ccagaggtcg tctctggatt
     8641 tttgctatgt tgcaaattta taaatcttct aaattaacta gcttcaaacc gtgttctgtc
     8701 atgcgataga tgatcgagca gacttctttt aataaacgac ggtcatgcga aacagtgatg
     8761 agaccgcctg gataggtagc aaagagtttt ctgatttggg gttgagaagt gggagaaaag
     8821 tttcgtgtgg gttcatccag caggagaaag tttggtttgc gcaggactaa atccaaaagc
     8881 aggagttttc cctgttgtcc gccagataag gagcgaattt gatgctgcat ttctggataa
     8941 ctgaaattga gactagctag gtgagattgg attttctgta gttcctcttt ttccccagtt
     9001 ttactgagat aggctattgg ggataaatcc aattgcagtt ttttgtggta atcttgtggc
     9061 ataaaaccaa gtgaaatctc tcttttatca ttcagaagtc tctgtaactt ggctaacaga
     9121 gttgatttcc caacaccatt tggcccgata ataccgattt tttcttggcc acggacagtt
     9181 agttgtagtt tttgaaccaa aactcggtcg tcaatggaca aattttcttt ttccagttgg
     9241 actaagactt tagaagctgg taatggttgg atgtctgaaa agaaaagttg gatttgttcc
     9301 tcttcaagtg gcttttgagt catggactga gctgcctttt cgtagcgttt ttcttgtgag
     9361 aggacagttt tcatcttttt agccaatagg cgaccggcag tactatcttt ggtagctcga
     9421 agcgcagttt ctacattttg cttaactctc cgatgttttt ccatggtttt atcgtaggct
     9481 ctttggttgt tagcagcttg ctgactttgt ttggcaaaat tagcctttct ctgctcacta
     9541 tagctatcat agtctaaatg ctctactagc gtttccgctt ctttacggtg tttgaccagt
     9601 cgcaagtgaa caatagtgtc tgccgtttca gaaagaaagt cttcatcatg ggaaatgaaa
     9661 ataacggttt gcctggtctt ttgaatctgg ccttttagcc aatcaactgt ctcaaggtct
     9721 aggtcatttg aaggttcatc taaaaataga atctcaaagg gtttggctaa ctcatggata
     9781 agctgaattt tcaaagcttc gccccctgat agattgccaa tctcttggtc acttgcgaaa
     9841 cgattgctat caaaatgcaa ttcctccgcc aaacgataga ggatactgta gtctaaatca
     9901 atagaatcta aaaagaagta gtcgtgtaaa gttttctttt ttaggtcctc agggactttt
     9961 tgaggaatgt aggccagtga ctgatagtca gattggatgt ttcccttgat agtgaaatca
    10021 gacaaagctt cccccattaa aattttaagt aaggttgatt tgccatttcc ttcttcacca
    10081 ataatagcaa ccttttcccc gtcttggatg gtcatggtta ggtcagatac aagatctcgt
    10141 aaatctttgt tttgtgtgat ggttagatga ttgattttta tcatatgatt tccctttcta
    10201 gaatgttcat agtgcataac aaaaagctct actttaccta gtagagccca aagtctatgt
    10261 caaaaaaact ctattaacat gccagaaaaa gagcataaaa gagcacacaa aaagagacaa
    10321 aaacaagatc tactaaataa aggttcttta tttgatagac ttagttgttc atgtctccct
    10381 tacctccgag tattagtatc tttatattat acctttaagg aaattttgtc aacagaaaac
    10441 tggaaattct atgctctaaa attcaaaaac aggtccatag agacttagta cgtgtttgac
    10501 atgatcgtaa tggaatttat catagtttcg ccaagcagtg cgtgcttgat cgtttagttt
    10561 taggctaccc agtccaatca gaagactggt aggttggtaa aatttctcaa gatcaattaa
    10621 gatactgttg ttcaactgtt ccgttttttt gagctcttgc atagtagtgt ttagcagctc
    10681 ttgtagtcga atatcatctg ccgtaccctg tttacagttt tggtagcttt tatttaactt
    10741 ggataggagt tgaatgagtt tcttgtcttc catgtgagca tcctccttga tctgatgtac
    10801 tctttcccta tagtatagca c
//
LOCUS       DAVLYV010000064        10702 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLYV010000064 DAVLYV010000000
VERSION     DAVLYV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.064"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(63..1487)
                     /locus_tag="VYO70_001592"
     CDS             complement(63..1487)
                     /locus_tag="VYO70_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1713800.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAVERAAYLHKTAAILERDKEEIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2032..4311)
                     /gene="pulA"
                     /locus_tag="VYO70_001593"
     CDS             complement(2032..4311)
                     /gene="pulA"
                     /locus_tag="VYO70_001593"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1713801.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKAYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4423..6381)
                     /gene="ligA"
                     /locus_tag="VYO70_001594"
     CDS             complement(4423..6381)
                     /gene="ligA"
                     /locus_tag="VYO70_001594"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1713802.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRSLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6474..7652)
                     /locus_tag="VYO70_001595"
     CDS             complement(6474..7652)
                     /locus_tag="VYO70_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1713803.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVCLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFFPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSLSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7732..8595)
                     /locus_tag="VYO70_001596"
     CDS             complement(7732..8595)
                     /locus_tag="VYO70_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1713804.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8598..10499)
                     /locus_tag="VYO70_001597"
     CDS             complement(8598..10499)
                     /locus_tag="VYO70_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1713805.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVEEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGDELDKLMSDYDHLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3203 a         2360 c         1915 g         3224 t
ORIGIN      
        1 taattattat tattttatag caaaaaaata aaaccaggaa aacaatttcc tggttttaca
       61 cgttatttca catcaaaaac aatggatttg acatttgtca tcgcttcaat gctatattta
      121 attccttgca ctccagctcc agaacctttg acaccaagga atgggaaatt atctggacca
      181 cgctgggttt tattattaat gtggactgta cctacttcaa gtttttcagc aatttcaaag
      241 gcttttttga aatcatttgt aaagactgat gattgaaggc cgaattcaga ttcgttggca
      301 aaggcaatag cttcctctac actagccaca cgaatgattg gtaaaacagg accaaatggc
      361 tcttcccatg ccactttcat atcttttgta acttggtcaa aaagcactgg ccagaggaga
      421 ttgccctcac gtttgattgg tgtaagagcc tgagcctctt tttcttgtgc atcctcaatc
      481 aagccccaaa tgaagtcggc tgaagcatta tcaataacag gtgtaatatc agcattgtca
      541 aatggatcac caactgttaa tttagaaact tcttcctgaa gcaaagtagc taatttatct
      601 gctacacttt cgagaacaat gacacgttta atggccgtgc aacgttgtcc tgagtagcta
      661 aaggctcccg caacaatttg cttggcagca tgttccaaat ctgcatcttc tagtacaaga
      721 gctgcatctt tcccaccaag ttccaacatg ataggacgca taccagctaa acgaccaata
      781 cgttctccaa taggagttga acctgtaaag ttgatgaagt tgacttcttt gtgctcaatg
      841 atataatccc caatttctga accacgacct gtaatggtgt tgaaaacacc tgccggaatc
      901 cctgcttctt caaatgcttt agccaacaag agtccagaaa tggaaccttg tgttggtggc
      961 ttaaacatga ccacattccc tgcaatcaag gcaggtgcaa ttttagaagc agataaatta
     1021 actggataat taaagggagc aatcgctagc acgataccaa ctggttcacg acggacaaca
     1081 gccagtttgt ttttacttgc tgcctcaaaa ccaccacctt ccattgcttg tccagtgata
     1141 cggagacctt cctcagcagc ataacgaatc aagtctgctg tacgcactac ttctccaatt
     1201 gctgctttaa tcccttttgc tacttctttg gcaaggatag taccaatttc ttccttatcg
     1261 cgttctaaaa tagctgctgt tttatgtaaa taagccgcac gttcaactgc tgataaagct
     1321 cgccatgctg gcagggctgc acgcgcagct tgcatagcct catcagcttc agtctgagtc
     1381 atggctggaa ctgtacccaa ttcttcttga ttgattggtg aataaatcgt aatttcttgt
     1441 tcagatgatt tccattttcc atttactaaa ttctgatatc ttgtcaaatt cttgtcctcc
     1501 tgaaaatgat taagatatat tttaccaaat tttcagaaaa tcacaaccct ttttgcaaaa
     1561 tttttgagat tattttcaca aacttgattt ttcaaagtat actcaataaa aataaaaaaa
     1621 atccactacg tcaaggcgag gctaatgtgg tttgaagaaa ttttcgaaga gcgtgaatga
     1681 gtatcatcta tagtaaaata aaaaaactga acaatttggt tggggacagc caaaccaatt
     1741 tctcacaatg tttcagaaac aagggtgtgc tactccaatt tcagcctact ataactgtca
     1801 tagattgctg aaacaaagtc taggtaaaag tcttcataat aaaaagacct cctatcaagt
     1861 gttcaaaaac tttgatagga ggtcttgttt tgtgaaaata tttatcaaat tttctataca
     1921 agtgagctgt tagccaggtt ctttctattc tttcaatttc aatgaatgga ttttttacta
     1981 atactcataa ctgggaattt gtctgtgtaa aaatagcgag atagatggta tttataaaac
     2041 actcaagaca gctagactaa tatcatttaa aacattatct tcttttgagc gactgttggt
     2101 taccaacata gctaaatttc ctgcattttc aaattgatag ggttctgatt tagcattcac
     2161 aaccaccaag aggtgttctt tgccgtgaac ttcatagata aggtagccgc tatgttcaat
     2221 cgcagaatgc acaaagacat gatggtaaat ttcatcatag ctagagtaag aaaaggcacc
     2281 agtttttgtc ttcaatcgga tgacttgacg gataaactca atactgtctt gacgctcatt
     2341 aatcaagttc cagttcactt ggttcacact gtcaggagca ttatagctat tcatcgcacg
     2401 ctctctatca tcatgggtca actcaccatt ttcaccagtc gcaaccagtt tggtacgacc
     2461 aaattcttga ccgatttcca taaaggccat cccctgcatg agcagattca tggctgtggc
     2521 agtttcgacc ttgcgcatga tttgctctga actttggtct ggatgaaggg ttgccaataa
     2581 atcgtgaaga ttgtaattgt catgggcttc tacatagtta agcacctgat ttggatgtgt
     2641 atagcttcct aattcacgac ttcctaggat tgctttagct agaattggct ctgtcgcagc
     2701 accactgaca aaacctgact tgatagcacc ataaacttct ccccctttga cagcatcgcg
     2761 ctgattgtca ttaaagaaac caatatttgg catctggtag gcattgtcct tcttggcctt
     2821 atcataaggg gcaagacctg ttcccatatc ccatccttct ccatagagga taatgttgga
     2881 gtcgatttca tccaagcttt gacgaatcat ctgcatggtc ttgacatcat gaatccccat
     2941 caagtcaaaa cggaagccgt caatattata ttcctgcacc cagtatagaa gagaatcaat
     3001 catatacttg cgaaacattt cgtgttcact ggctgtttca tttccaacac ccgttccatt
     3061 ctggaaggta ccatctggat tcatacgata atagtaatca gggactgttg tttggaatgg
     3121 tgcatcaaca actgagaagg tatggttata gactacatcc ataatgactc caatacccgc
     3181 atcgtgataa gcttgaacca tcaccttcaa atcacgaatg acctgagctg gatcatctgg
     3241 attagttgaa aaactagttt ctggcgcgtt atagttttgt ggatcataac cccagttgta
     3301 ggttacattt ccatcctcat cgtattcttt atgacggtct gcaattggtt gcaattgaac
     3361 ataattgtag cccagcttct tgatgtaatc aaaagcagtt gactggccgt attggttaac
     3421 tgttccagcc tgagcagcac ccaagaaagt tcctcgaaga tgttcatcta cacccgatgt
     3481 aggtgattta gtcaaatcac gaatatgcat ttcacagata actgccttac atggattttc
     3541 caagcgccaa gtagcctccg aaccgtgctt aacctcgaag ttttcaactt gcttttctac
     3601 atggctcaga atagctgaac gtttgccatc agggctggtc gcgattgtat aaggatcacg
     3661 tgtcagtgtt tggtgatgag ggaattggac ttgatactga taagccttac ctaccaaatc
     3721 ttcctcaaca tccaaactcc agacaccgat tgtattgtcc ttatgattat aagagtagct
     3781 attgcctctt ttcatctcaa aagtcttcca aacgggtgca tcattagcag ctgattcata
     3841 aacgacaact tgcacttctg tcgctgtagg tgaccagagg gaaaaatgag cctgattgtc
     3901 ctctacacgg caacccaatt ctccttggta accccaatga tggtcaaaac tagcactgtt
     3961 aatggcctta tcaaaggcaa aaggattttg atttttatag aaaggactgg caatagcagg
     4021 attttcagag taataaatcc tatcatcgcc ttccaaaatc cagacctctg ttaatagggg
     4081 atagtgatta aaacggatag aatattcttt actagtttga cctgtatgaa ccacaaaatt
     4141 caagctttct ataacatgtg aacttgggtg ttcaaagcta aataaagctc caaaataatc
     4201 ttctttgtag gttagcaaat caattcgttg atcctgactt tttacaaagg agcaagtgtc
     4261 atattctcca ttcttacgat ggtaatgaat gcgcataggg tagttataca ttttttattt
     4321 ttccttttta ctttgtttct atttcactaa taaatttttg tcaatctcgt ctcaattaac
     4381 agacatagtc atattctcta aactctgttt ttaaacgatc cattacaaac tttctagcca
     4441 tgcctcatct ctgacctgga taccaagttc ttgtgctttt tgcagtttac ttccagcgtc
     4501 tgcacctacc acgacgaggt cggtcttttt agaaacacta cctgtcactt tggcacccag
     4561 actttcgagt ttacttttag cttctgagcg cttgagtcgt tccaattttc ctgtcaatac
     4621 cacggtcaaa cctgacaagg ccgcatccgc tactaccgtc tgtcctttat agtccagatt
     4681 gaccccagtt tctttcaatt ctctgagcag aatttcagag ccttctgtcg caaaataagt
     4741 ctgaagactt ttggcaatca cgccacctag actttcaata ctagccactt cctctgaatc
     4801 tgcctgagac agattttcaa ttgaatggaa atattgaagt aaaagctgac tagccttgct
     4861 tccgacatga cgaattccca aaccaaataa gagcttctcg gcagaatttt cctttgatgc
     4921 ttggatagcc tgatacagtt tagcagcgga cttttcctta accccctcta aaaggaggaa
     4981 atcctcttct tgcaaacgat aaatatccgc cacatccttg actaaattag cagcaaaaag
     5041 cttctcaaca atagatggac caaggcctgt aatattcata gcatcacgag aagcaaagtg
     5101 aatcaagcct tccatgattt gagcagggca acgcggattg atacaacgta gggccacttc
     5161 atcttcaaag tgcaacaagt cagagttaca acttggacag tttgtaggga tatctagttt
     5221 ttcttcagaa acccgtttgg actctaccac acgtaaaacg gcagggatga tgtcaccagc
     5281 cttatataca atgaccgtat cgtcttttcg gatatctttt tcagcaatat aatctacatt
     5341 gtgcagggtc gcacggctaa cagtcgtacc ggcaagttgt actggtgtta gattagcagt
     5401 tggagttaca acaccggtac ggccaactgt ccagtcaact gataagagtt gagcttcttt
     5461 ttcttcggca gggaacttgt aggctactgc ccactttgga gccttaactg taaaaccaag
     5521 ttcttcttga cttgctaggt cgttgacctt gattaccact ccatcaatat cgtaaggcag
     5581 attttcccgt tcctgtccta cttcttggat aaaattccag atttcatcta tgttttcagc
     5641 caagattcgc ttaggattga ccacaaaacc tagttgttct aggtacttca aacccttttc
     5701 ttggctatca cgagttgaag ggctggcttc ttgatagaga aacgttgcaa gactacgctt
     5761 ggcaactact gctgtatcca actgacgcag agttcctgct gccgcattac gaggattagc
     5821 aaattcaggc tctccatttt cttggcgcac ttggttaact tggtcaaagg aagcgcgtgg
     5881 catgtaacat tccccacgaa ctgtgatatc tagttcttct ggcaaagtca aagggatgtc
     5941 cttaacacgc ttgaggtttt ctgtgatatt ttcaccaatt gaaccatctc cacgtgttac
     6001 cccagcaacc aaaatcccct tttcataagt cagcgagata gataagccat cgattttcag
     6061 ctcacaaata taggtcggat gagccacttc cttacgaaca cgcgcatcaa aagcatctag
     6121 ctcctcacgt gaaaaagcat cctgcaaact ataaagagga tactgatgac tgtatttttc
     6181 aaaaccatct aaaaccttgc caccaacacg atgagtcgga ctgtctgcta gcacttgctc
     6241 tggataagca gtttctaact cgaccaactc acggtaaagg cggtcatact cactgtctga
     6301 aaccgaggga ttatcgctgg tatagtactc agtcgcatag cgattgagca aagcgactaa
     6361 ctcattcatt cttttattca taagaccatt ttaccataaa acaagccctc ctcacaaacg
     6421 agaagggcgg aaaaaacact tagtttgaaa ttatttttga aactcaagca accttatatc
     6481 aatttttcaa aatgagttcg aacataaata aacgatatac aagacaagat gataacacca
     6541 cttccaatta tcaggaaaga agagagatgt acacttggca agactgtcat aaatcctttt
     6601 gcaataggca taaatagaat agctaaggta aaaattgtac tcagtactct cccaagaaat
     6661 tcgctctcaa ccttggtttg tacttgagta aaaaagtgaa tattaaaaat cgtcataaac
     6721 aattcacaaa ctaaatttcc agaaagggaa agaaaagttg gaagtggtaa tcccatcata
     6781 aaaactccga cacctgtcaa agccagtaaa atcaaaagat tataaatatt agctttaatt
     6841 ttactagcta gaagagcccc aatgatggaa ccaatagccc ccatagttaa aatacttgca
     6901 taggctcctt ctgacccgta aagctgattc gaaaagggaa gtagaaattc aaaagctgca
     6961 aaaaagaaat taacgctgga agctaccagc aaaaggaaga aaatttcttg ctgatgccag
     7021 atatagtgta acccatcctt gatatctaca aaaatatctc tcccagtaaa agcctttttc
     7081 tcttgaactt ttgcttcctc ttttggaaag aaagccacta gaacaaaagc aatgaaaaaa
     7141 gtcagcgagt ctagcagtag cgtcatatgg agacttgcaa actgtaaaac aaggaaggaa
     7201 agaacaggag agctaacacc tacaacctgc aaaaccagct ctaggcgaga attatagatc
     7261 acaatctcat ctttctccac cacttcagtt atgatagctt tattggctgt gcgagaaaag
     7321 gcaaaagcaa tagcctgcac aatgttagca acaatcaaag cgccaatcat ccagctatca
     7381 ttccttatga aagaaatagc cagacaaaga atcccacaaa caagatctgc cgtcattaaa
     7441 atcttacgac gagaaaaacg gtctgaaata actccgccaa agggattgac gagaatagat
     7501 gtgacgagct cagaaatctg atacattcct aaaactgtct gtcctatagt ccccatagaa
     7561 gccaagcaga cactatttcc ataatcatag agcatatttc ccattttatt gatagcccca
     7621 cggctaatca actgcactgc atagcgattc atattaaagc tcctctcaaa ttttgaaact
     7681 attgtatcaa aaccgaaagg agctttttat tttttccctt atttgggaaa attaactttt
     7741 gacaaatttt tcgtagtgtt cctgataata ggctacttgc tctggaagac ctaacacatc
     7801 aaaaatatgt atggcctctt gcatctgctt acagccttct ttacactgtc ctttttgata
     7861 taaggcaaaa ccttttaaat aatggaaaac attacgctca taaagcttaa tacctttgtc
     7921 aataatcttc tctgtataag cctcaaaata gttggcatta taaaaagaag aatgctctaa
     7981 acaatgctgg taacaattga gggccaaaat caacactaat ctcttatggc gactaatctc
     8041 ttggtaaaat tcctccctct ccataacttc tctaccaatc cgagtgacat agtctacatc
     8101 gtagaaacta tagaggttac cgaaaagaat caactcatac atggtccatt cttctgtttt
     8161 gaagagataa tctgctacct tatccaaatc atcctgcttc atatcataac tcgcatctct
     8221 ttgacaaatc agaccttgta gcaaaatcca gttcagctca aaataaaggg aagtcgtcga
     8281 actcttagac ttttcaagtt gttctctttg aagcttttga aaacctgcaa tatcgtttga
     8341 atagtaaagt gggataatct gtgccatcat agacacatgt tcatgattat gaaaattcct
     8401 tgccttatcc atgaaatttt cgattgttac atgaatgtta tccaaaatct caaagaaacg
     8461 ggagactgcc aggtcagact ccccaagctc aaagcgagat aactgagagg tagagcagga
     8521 ttcgcctgct gcttccttta aagaataatt tccacttgtt cgaaattcac gaaatacttt
     8581 tccaagatgt tccatcttta cacctgatct gataattctt cccactcaag catagcttct
     8641 tcctgacgat ggctgatttt gtccagctca gcctgtaatt ccatgagttt gtcggcatcg
     8701 tttgtttcca acatttgttc agaaatggct tggctttgac tttctagctc ttcaatttca
     8761 gcttctagac tttcgatttg tcgcatgagt ttgcgaactt ctttttgact ttctttctgg
     8821 gcctgatagt cattgactgg acttgcttcc tttgcttgat tgctagttga agcttcctca
     8881 gtctgactca tttctgctgt tgctttcttc tcaacatagt agtcgtaatc tccaaggtag
     8941 agagttgaac cattctcaga caattccaaa acatgagttg ccacacgatt gataaagtaa
     9001 cgatcatgac tgacaaaaag caaggtacca tcaaagtcaa tcaaggcatt ttctagcact
     9061 tccttactat caatatccaa gtggttggtc ggctcatcca gaatcaaaaa gttattgttt
     9121 tccatagaca atttagctaa aagcaaacga gctttttcgc caccagatag catgccgact
     9181 gattttttaa catcatctcc tgagaaaagg aaggctccaa gacggttgcg gatttcaact
     9241 tctggtgtca gtttgaaatc attccagagt tcatccagca ccgtattact tggtgtcagc
     9301 ttgctttggg tttggtcata gtaaccaacc tcaacattag cgccaaagcg cttttctccc
     9361 ttgataaacg gaatttgatc cacgattgat ttgataaagg ttgattttcc gataccattg
     9421 ggaccaacga tagcaacagc attcatctta cgaaggtcta ggttaatcgg ttgtgacagg
     9481 acttccccat catagccaac agccgcattt tcaacagtca aaacaacatt gcccgacgtt
     9541 ttttcagact ggaaggtcat gttggctgct ttcttgccag cttccggctt gtccaaacgc
     9601 tccatttttt ctagttgttt acggcgagat tgagcacgtt tggtcgttga agcacgaact
     9661 agattgcgat tgacaaagtc ttccagagca gcgatttcct tctgttgctt ttcatagttt
     9721 tttgcctcag tagctagctt ttgctccttc aactcgacaa aacgagagta attccccaca
     9781 tagcgatcca aggaatgctt ggtcaaatct agcgtaattg tcgcaacctt gtccaagaaa
     9841 taacggtcgt ggctgacgat aatgagggca ccgctatagt ttaccaagta attctctagc
     9901 caggagatgg tttcaatatc caagtggtta gttggctcgt ccaagaccaa gagattgggc
     9961 ttttcaagga gcattttagc tagagccaaa cgagtatttt gaccacctga aagttcagct
    10021 attttcatct gccacataga ctcgtcaaac ttgaatccat tcaaaatcgc tcgaatatca
    10081 gcttcatagg taaagccgcc tgcttggcga aaattctcag ataagtggtc gtaatctgac
    10141 atcagtttat ccaattcatc gccagacttt tcacccatct ccaactccat ctgacgaagt
    10201 tgtttatctg tccgacgtaa gtcatcaaag acatgaagca tttcatcgta gatggtattt
    10261 tcagactcaa aacggctatc ttgggctagg taagacagag aaatatcttt tttcttattg
    10321 atttctccgc tagttggctc ctcttcttca actaaaatct tcaaaagagt agacttacct
    10381 gcaccatttt tcccaacaag agcaatccga tctcgttcat caacctgcag gttgatatta
    10441 tcgaaaagaa cctctcctgc aaaagaacgt tcaattttat tagcttgtaa aataatcata
    10501 agagtagtat agcatgtttc cctaaggcat tcaagataat cgtaagtctt ttagtacaac
    10561 ttttataaca taaaataaac taaattatgt atattttata ttagattcct tcactatctt
    10621 gttggatttt ctaaccagct aatcttgttt caaatagtta tcgcacaagt ctattattta
    10681 attcttttca tcatttacgt ac
//
LOCUS       DAVLYV010000065        10661 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLYV010000065 DAVLYV010000000
VERSION     DAVLYV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.065"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(48..1370)
                     /locus_tag="VYO70_001598"
     CDS             complement(48..1370)
                     /locus_tag="VYO70_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1713806.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(1461..1549)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(1713..4049)
                     /locus_tag="VYO70_001599"
     CDS             complement(1713..4049)
                     /locus_tag="VYO70_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1713807.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVIAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(4153..4701)
                     /locus_tag="VYO70_001600"
     CDS             complement(4153..4701)
                     /locus_tag="VYO70_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1713808.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(4698..5000)
                     /gene="zapA"
                     /locus_tag="VYO70_001601"
     CDS             complement(4698..5000)
                     /gene="zapA"
                     /locus_tag="VYO70_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1713809.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            5087..5968
                     /locus_tag="VYO70_001602"
     CDS             5087..5968
                     /locus_tag="VYO70_001602"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1713810.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            5972..6586
                     /gene="lepB"
                     /locus_tag="VYO70_001603"
     CDS             5972..6586
                     /gene="lepB"
                     /locus_tag="VYO70_001603"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1713811.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            6714..9080
                     /locus_tag="VYO70_001604"
     CDS             6714..9080
                     /locus_tag="VYO70_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1713812.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPGQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLIRILEKGKQKSQDLETIQKHITTVEQELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNSIPAHDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(9128..10411)
                     /locus_tag="VYO70_001605"
     CDS             complement(9128..10411)
                     /locus_tag="VYO70_001605"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1713813.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT         3030 a         2435 c         2161 g         3035 t
ORIGIN      
        1 taaaaattat ttataggcaa aaaaatcaca cgagctgtgt gattccatta tttgtcaaaa
       61 tactttttag tttcagcaat aacgactggc gacaagacca agagggcaat caagtttggc
      121 agagccatca aggcgttaac gatatctgcg ataatccaga ccatatccaa ctcgataaat
      181 cctcctaaca agaccatgag cacaaaaacc acacggtaga gccagataaa gcgagcccca
      241 aagaggaact caaaacagcg ttctccgtaa tagttccaac ctagaatcgt tgtaaaggca
      301 aaaagtacaa ggaagatggt caagagggca ggcccaaagt gtgaaaagac tgttgagaaa
      361 gctgactgag tcaaggcaac cccattcaag tcaccactcc aaactccagt taccaagatg
      421 gtcaaaccag ttagagtaca aatgatgagg gtatcaataa aggttcctgt catggaaatc
      481 aaaccttgct ctactggttc atttgtcttg gcagctgcag ctgcaatagg agcagaaccc
      541 agaccagatt cgtttgagaa cacaccacgc gccacaccat tttgaatagc catccgaacg
      601 ctagcaccag caaatccacc taccgcagca aggggactaa aagctgaggt aaagactaaa
      661 gcgattgtgc cagggatttt tccgatatta aagaaaataa ctgtaagagt tcctaagata
      721 taaatgaggg ccataaaagg aacaacagta gttgaaacct tagaaataga cttgagtcca
      781 ccaaagactg caatcgctac aaagacagac aagacgagag ctgtgatggc tggcgaaatc
      841 gtcgttgtat tttggataga ttctgtaatc gagttgactt gggtgaaggt tccgattccc
      901 aagagagcaa ccaatactcc tgctactgca aacaaaacag caagtggtcg ccacttttct
      961 cccatcccta gaaggatata gtgcatggga cctcccgcta ctgcaccatg gtcgtccttg
     1021 gtgcggtatt tgatggccaa gagtccttcc gcatacttgg tagccattcc aaagaaagcc
     1081 gccatccaca tccaaaatag agctcctggt ccaccaacct tgatagccgt cgcaactcct
     1141 atgatatttc ctgttccaac agttgatgcc aaggctgtac acagagctgc aaaactggat
     1201 acatcaccat gtcccttatc ctggataaaa ataagctgaa aggccttggg cagacgcaaa
     1261 acctgcaaga gtcctagccg catggttagg taaatccctg ttccgaccaa taaaatcaag
     1321 aggggcggtc cccaagcaaa agcatcgatt gatttaagca attctaacat ttccttctcc
     1381 tatcgtttca accccaaaag aaagagcaca tgcaagatac atgtactctg gaatgcttag
     1441 ataaatgcta aaaagcggtc tatcctagct ctgtcctttt acctgagagt ttgagcagtt
     1501 gcctgccttg ccccttcggt gcctttacgg tctctccaga gttccgtcca tttacagtca
     1561 tggaaaatca aacgattccc cacttctatt aaacttcatt cggtgttggt atttaattga
     1621 ttctaatttt acaaaaaatg ttggcttttg tcaatgtgtt tattagtaaa aattagttca
     1681 acagttttta ctttataaag tccagaatac tgctatcctt taaaagtgac aatagtcgca
     1741 ccactgcctc cagcattttg tggggcatag ccgaaactct tgacatgttt gtttctttgc
     1801 aagtatttgg taactccttc acggatgact cctgttccga taccatggat gatatcaact
     1861 tgagccatat tgttaagcaa ggcttggtcg atgaaggtat ctagctcatt catggcttct
     1921 tcatagcgct tgcctcgaag atccagtcta gcttgaggtc ctcgcccaga agttcgtttc
     1981 acaacattga cctgtttctt cttgactggt ttttcttgct gggcttgaac aagatcaaac
     2041 tctttctctt ccaaggtcat cttaatcaag ccaacttggg cttcccagcg accgtccttg
     2101 agttgactgg tcaaggtacc acgctgacca taactgagaa ccacgatatc atctcccacc
     2161 tttggagctc gttttttctt ggccttttga aggaccttat ttttagacaa gtccactttt
     2221 tcaggagcca attttttcaa cttggccttg gcttcaatga tttcgtgggg cttgagttgg
     2281 gatttactgt ggagattttt gagaatctgg tcactttcac ttagggccat atccacaatc
     2341 tcagcagcct gttcacgcgc cttgttaagc tcggtttcct tttcacgatt aagctcgttg
     2401 tagagttttt ttagcgcacg gttcatcttg agattttctt gctccacctc acggatattg
     2461 tccaaacgtt tgcggctttc cagcgtctgc tcttctaatt gctcaatgat acgattgacg
     2521 tcattgtcct gatcgatctg ctgactggca tctcctacga taacttcaga taggcctaga
     2581 cgtttggcaa tttcaaaggc attacttcgg ccaggaacac cctgcataaa gcgataggtc
     2641 gggcgaagag ttgcagtatc aaactccata ctggcatttt gcacaaaggc tgtctcaata
     2701 ccgtaggcct tgagttctgg atagtgggtc gtcgccatgg tcttgatttg acgcaggcga
     2761 aggtcctcca gaatagccat ggcaagggct gctccctctt ggggatcagt accagccccc
     2821 aactcatcca aaagtaagag tgaatgttgg ttgaccttgc caagaatatc cacgatattg
     2881 gtcatatggc tagagaaggt agacaagctc tgctcaatag actgctcatc tccaatatca
     2941 gcaaagattt cttcaaaaat accaacacga cttcccttgt ctgctaaaat cggcaatcct
     3001 gactgggcta tgacctgtgt caagcccaga gttttgagca tgatggtctt cccacctgta
     3061 ttgggacctg taatgacaat agctgttaaa tcttgaccaa aatggacatc atttgcgacg
     3121 gcatttttga ccaaaggatg gcagacatgg agcagttgaa tctcttgatt ttctgacagc
     3181 tgaggcacga ctgcttgtct ttcttggata aatcgaacct tggcacgaat caagtccaga
     3241 tgaccgataa tccaagcgtc attagcaatc tcagccgcat gagggcggac acgctcagaa
     3301 atttcttgga gaatgcgaag catttcatag cgctcatctg ctcgcagact agcaatttct
     3361 tcgctcagtt tgactacctc acgtggttcg atatagacgg tgtttccact agcagaaata
     3421 tcatgaacga cacctgcaat cttattgcgg taggtgtttt tgactggtaa aacctgacgg
     3481 ccatttctgc tagcaacaat tccttccgtc aacatctgcg ctttttgctt gagcaagtct
     3541 tgtaaaacat cgcgtacctg actctcgcta tcatgtattt ttcgacggat tcgcgccaat
     3601 tcttcactgg caaaattttc aatgaaaccc gcatcattaa aggcctgaag atttccttgt
     3661 aattgcggaa aatcatgtaa tttctcaaac caaagggcta attcttccaa gctgacattt
     3721 tccagattgg tgtaaaaatt ttgaagttct cggctggcaa gaagcacgcg tttcaagagt
     3781 aggaactcct cgatattgag atccgctccc atctccaacc tcttgcagac tcctgcaatt
     3841 tccttagttg agagaatagt aaaatgcggt tgctcgacga aaagagcctg catttccttc
     3901 atctcagcaa aagcctgttt gattttatct gctttggcag tcggagccag ttgtctcaat
     3961 tgctccaagc cctgctcggt caacaaatga ggctcaaaca aggccttgac cttatcgaac
     4021 tctaatgttt ctaatatttt cttattcatc tttatttcct tgtctttgaa tgtctaggtg
     4081 tggtagcttt ttacattctg atagattcga gtcaaatctg taaacaaagg gctaggaaaa
     4141 ctcctgccct ttttatccga ttaaatttgt cacccagatt tgtttgagcc aactggttgt
     4201 tatcggtatg ctctggatga tgtgttttgc gacgatactc ttttcaagag gattttgtat
     4261 aactgccatg gggatggtcg ccaagattgt caaggccatt tgcaagacaa ataaggtcac
     4321 caacatggac aagatacctg ctgaaacttg gaacaactta ccacccagtt ttttgctagg
     4381 aatcaagtgt aagagaagac caagcaaacg accaatgcta tagacaatcc caaatacaag
     4441 caagtagccg atacctgcat aaaagacctt atccagctga aagagttgat ccgatgggaa
     4501 aaagaaagtc ccctgacctt cctgcgaatt tgcataaggg agcaataaat ggaattgctc
     4561 tccaagcccc ttataaaact ggccagccat aaaagccgat gccatggctg aaatcaggta
     4621 ataaacctgt aagagcaggc ctctccgata gccgatataa aatccccaaa ccaagaccaa
     4681 tagaagaagg aatgaaatca taaggaatcc tcaatcttgc tctgttcttg cttacaagtc
     4741 acaagcttgt gacggagttc ttctagctct tgctccttat cgtcaaattc aatctcacgg
     4801 ctgagctgag ttgataaaca gttgactgcc aacaaaagag cgattgtttc atcatctgcg
     4861 ctaggcattt gttctttaat tgcttggtat ttttctgtcg caaccttagc gatttcctcc
     4921 ataaaaaggt tatcatgttc gcttgtcaag gttaacgatt ttttcccgaa tgtaaatttg
     4981 aatcgattta gatttgccat aaaattcacc tcacgatatt ataccaaaat tcgctaattt
     5041 tgtcagtttt tacaaatttt actgcttttg tggtacaata gaaactatgg caagtatcac
     5101 actcacacca agcgaaaagg atattcaggc ttttcttgaa cactatcaaa ccagtctggc
     5161 tcccagtaag aatccctata tccgctactt tttgaaacta cctcaagcaa cggtttctat
     5221 ctatacttct ggaaaaatct tgcttcaggg tgaaggggct gagaaatacg ctagtttctt
     5281 tggctatcaa gctgtagagc aaaccagcgg acaaaatctt cctttaattg ggacagatga
     5341 ggtgggaaat ggttcctact ttggtgggct tgcagttgtg gctgcctttg tcacatctga
     5401 ccagcacgac tttttacgaa aactcggtgt gggggattct aagactctga ccgaccaaaa
     5461 gatccgtcag attgctccta ttctcaagga aaaaatccag caccaggcac tccttctctc
     5521 acccagcaag tacaacgagg tcatcggaga ccgctacaat gctgtttcgg ttaaggttgc
     5581 cctccataat caggctatct atctcctcct tcaaaaaggt gttcagcctg agaaaattgt
     5641 gattgatgcc tttaccagtg ctaaaaatta tgacaagtac ttggcacaag aggccaatcg
     5701 tttcagcaat cctatcagct tagaagaaaa ggctgagggc aaatacttgt ctgtcgcagt
     5761 ttcttctgtc attgcgcgtg atctctttct ggaaaatctt gaaaatttgg gacgagaact
     5821 gggttatcag ctcccaagtg gagctggaac ggcttctgac aaggtggcta gccagatttt
     5881 gcaagcctat ggtatgcagg gactcagctt ctgcgccaaa ttgcacttta aaaacactga
     5941 aaaagcgaaa aaacgcttag aaaggtaagt tatgaattca tttaaaaatt tcttaaaaga
     6001 gtggggattg ttcctcctaa ttctgtcatt actagcttta agtcgtatct ttttttggag
     6061 caatgttcgc gtagaaggac attccatgga tccgacccta gcggatggcg aaattctctt
     6121 cgttgtaaaa caccttccta ttgaccgttt tgatatcgtg gtggcccatg aggaagatgg
     6181 caataaggac atcgtcaagc gcgtgattgg aatgcctggc gacaccattc gttacgaaaa
     6241 tgataaactc tacatcaatg acaaagaaac ggacgagcct tatctagcag actatatcaa
     6301 acgcttcaag gatgacaaac tccaaagcac ttactcaggc aagggctttg aaggaaataa
     6361 aggaactttc tttagaagta tcgctcaaaa agcccaagcc ttcacagttg atgtcaacta
     6421 caacaccaac tttagcttta ctgttccaga aggagaatac cttctcctcg gagatgaccg
     6481 cttggtttcg agcgacagcc gccacgtagg taccttcaaa gcaaaagata tcacagggga
     6541 agctaaattc cgcttctggc caatcacccg tatcggaaca ttttaagaaa cctaagaggc
     6601 cgagaatcac caatctcagc ctcttcttct atcgtgagaa aatgattggt aactatctaa
     6661 acttaccaga acagaaacac ctcaactctc acctattcat gcaaaggaat tttatggaag
     6721 tttatttttc aggaaccatt gaacggatta tttttgaaaa tcccagcaat ttttatcgca
     6781 tcctcctcct agaaatcgac gatacggacg cagaggattt tgatgatttt gaaatcattg
     6841 tcacaggaac catggctgat gtaattgagg gcgaagacta tactttttgg gggcaaattg
     6901 tccagcactc caagtatgga gaacaactgc aaatcagtcg ttatgatcgc gcaaaaccaa
     6961 ctagtaaggg cttggtcaag tacttttcaa gtagccattt caaggggatt ggtctcaaga
     7021 cagctcagaa aatcgtggat acctatggcg aaaataccat tgacgaaatt ttgcaacacc
     7081 cagaaaagtt agaaggcatc gcaggactct ctgccaaaaa tcgcgaggct ttcgtctcca
     7141 ctctccgtct caactacgga acggagatgg ttttgtccaa actagccaac tacggcattc
     7201 ccaacaaact agcctttcag attcaagact tttacaagga agaaaccctt gatgtggttg
     7261 aaaattatcc ctaccagctg gttgaggata tcaagggttt gggctttacc attgctgacc
     7321 aactagctga ggaactaggc atcgaaagtc aggctcctga acgcttccgc gccggtctag
     7381 ttcacagtct ttttcaggcc tgtatggaaa caggggacac ctatgttgaa gcacgggatt
     7441 tgctggaaca aacccttact ctccttgagt cttcccgtcc cgtggaacta gaccccggcc
     7501 aagttgccca agagctctcc tacctgatcg aagaagacaa ggttcagcag attgatacca
     7561 agatctttga caacagcctc tttttcgctg aggaaggtat ccgcagtcac ttgattcgta
     7621 tccttgaaaa aggaaaacag aagagccaag atttagaaac tattcaaaag catatcacta
     7681 ctgtcgagca agaactgggg attgagtatg ataacattca aaaacaggct atctgtgatg
     7741 ctatccagaa taaggtcttt atcctgacag gtggacctgg tactggtaag acaactgtta
     7801 tcaatgggat catcgctgtt tacgccctgt tagagggact tgacctcagg aagaaaagca
     7861 acctgcccat tcttcttgct gctccaactg gtcgagctgc tcgtcgcatg aatgaattga
     7921 caggtttgcc tagcgcgacc atacaccgcc acttgggaat gacaggtgac gatgatacca
     7981 gtcatctgga agattaccta gatgctgact ttatcatcgt ggatgaattc tctatggtgg
     8041 atacttggct ggccaatcaa ctcttctcca atatctcttc taacagtaag atcctcatcg
     8101 taggtgacag tgatcagcta ccttctgtca gtcctggaca ggttctagcg gatctgcttc
     8161 atattccttt gattcctcag actcgcttgg aaaaaattta tcgacaaagc gaagaatcaa
     8221 ccatcgtcac cctagctagt cagattcgac agggcatctt gccagctgat ttcacccaaa
     8281 aaaaagctga ccgttcctac tttgaaattg ctagtggcca tattcctgcc actattgaaa
     8341 aaatcttagg tgccgccctc agaaatagta ttcctgccca tgatatccaa gttctggctc
     8401 ctatgtaccg agggacggca gggattgatg ctatcaatca gctcatgcaa gacctgctca
     8461 atcccccaca aaaagatcaa ctcagttttg aagctcctca gtgccactat cgtaagggag
     8521 acaaggtcat tcatttggtt aacgatgctg aaatcaatgt ctttaatggg gatttaggag
     8581 ctatcacaga cctgattcct ggtaaataca ccgaatcgaa acaagacgag attgtcattg
     8641 attttgatgg taatgaagtc tcttaccccc gtaacgaatg gtacaagatt cgcttggcct
     8701 atgccatgag tattcataag tctcagggaa gtgagttccc agttgtcatc ctaccgatta
     8761 ctagtgctag ccgacgtatg ctggagcgaa atctcatcta cacagccatt acacgtgcca
     8821 aaagcaaact catcttacta ggcgaattac aagccttcga ctatgctacc caacacatcg
     8881 gaactgcccg aaaaacctat ctgattgaac gcttcagtga tctactggag aatgttgaag
     8941 aaaagcaaca agctgtctct gaaacagtca catcaagtgc ctctgaacaa tcctacatcc
     9001 taaccgaaga aaactgggac cgcatcccag ccatgattgg gattacagac acagacctca
     9061 aagagatttt tggaaaatag tgcatcagaa aacccaccaa ttcaggtggg ttttctgttt
     9121 attaagatta ttttactgtt gctgtgcttg tgatcaattc aacagctttt ttgatagtga
     9181 tatcatgttt caacatgtca gctgaaagca agttttgaac ttgtgcaact tccatgttgt
     9241 agtctgctgc caattgctca acttcttttt ggatttcttc ttctgaagca tcaaatcctt
     9301 cagctttggc aactgcttcg ataacaaggt tagtcttagt acgtgactca gcttctgctt
     9361 ggtattggtt gtgaaggtct tcttgagtag ttccagtgat ttggaagtac atgtcagggt
     9421 tgatcccttg acgttgcaaa ttcccaagga attcatttac tgaacggtga acttcttcat
     9481 ggatcatttc ttctggaagt tctacgattt cagcattttc tacagctgta tcaattgctg
     9541 caccttcaac tgcatctttg taagcttctt ctttagcagc agccaattct ttgcggtatt
     9601 tttctttcaa gtcagcaagt gtttcaactt cttcatcaat gtcttttgca agttcatcgt
     9661 caagagctgg aacttcttta gcttttactt cgtggatagt tgtcacgaat ttagcttctt
     9721 tacctgcaag gtcttctgct tggtagtctt ctgggaatgt tacgataaca tcaacggttt
     9781 cgccagctga gtgacctacc aattggtctt cgaaaccagg gatgaattga cctgaaccaa
     9841 gtccaagtga gaagttttca ccttttccgc cgtcaaattc aacaccgtcg atagaaccaa
     9901 cgaagtcgat cacaacagtg tcgccgtttt cagcagcagc ttccttgata accaattcag
     9961 ccaggttgtt gcgttcgcgt tcgatacgct cttcaacatc agcgtcagtt acttcttttt
    10021 ctacatcaac tgatacttca aggtttttgt agtcacccaa ttttacttca ggttttgtaa
    10081 cgacttcagc agcgataacc cagtcttgac ctttttccat tgaagttacg tcaatttttg
    10141 gttgggcaac cacttcaaga ccagcttctt ttacagctgc ttcataagcg tttggcaaaa
    10201 gagcgttcat aacgtcttgg taaagtgatt cttcaccaaa ttttttatcg aagataggac
    10261 gtggaaggtg acctttacgg aaacctggaa cattaagaga tttcttcact gacttgaaga
    10321 cacggtccaa ttctggtttg atttggtctt gagagatagt gaaagtcaag acaccacggt
    10381 ttgtttcttt gttttcaaat gatacagaca ttctgtcatt tctccttaaa attttttaaa
    10441 tacagtctat tataacattt tctataaata aaatctagac taatgcagcc aattatagac
    10501 aacaagcctt tttcctaact aaaacactca taaagtatag acaattttag actactttgg
    10561 atatcttttg gttagtcatt ttccaaaact aatttatcta atttttcagt tatatttgac
    10621 ttcatcttct cagtaacatg agtataaaca cttaaaatct t
//
LOCUS       DAVLYV010000066        10596 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLYV010000066 DAVLYV010000000
VERSION     DAVLYV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10596
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.066"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..965
                     /locus_tag="VYO70_001606"
     CDS             <1..965
                     /locus_tag="VYO70_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1713814.1"
                     /translation="LTIDSRRLIERWKKEGKSNREIASLLGKVPQTIHTEIKRGTVRQ
                     CLGKGRFKEVYSADYAQQSYENNRKRSVRKSSVTKELKEKILHYHNQKFSPEMMVMVK
                     GVNVGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQSIEQRSEAINL
                     RLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSADVVNQALKLILKQHK
                     ILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRRWLPKGIKKMTP
                     KEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
     gene            991..1634
                     /locus_tag="VYO70_001607"
                     /pseudo
     CDS             991..1634
                     /locus_tag="VYO70_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
     gene            complement(1676..3664)
                     /locus_tag="VYO70_001608"
     CDS             complement(1676..3664)
                     /locus_tag="VYO70_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1713815.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVIAVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSDIYNQFYGGMNVSISEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3666..4424)
                     /locus_tag="VYO70_001609"
     CDS             complement(3666..4424)
                     /locus_tag="VYO70_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713816.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4852..5043
                     /locus_tag="VYO70_001610"
     CDS             4852..5043
                     /locus_tag="VYO70_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713817.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(5397..5669)
                     /locus_tag="VYO70_001611"
     CDS             complement(5397..5669)
                     /locus_tag="VYO70_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1713818.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5728..6177)
                     /locus_tag="VYO70_001612"
     CDS             complement(5728..6177)
                     /locus_tag="VYO70_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1713819.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(6164..8293)
                     /locus_tag="VYO70_001613"
     CDS             complement(6164..8293)
                     /locus_tag="VYO70_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1713820.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFVTRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            8337..8420
                     /locus_tag="VYO70_001614"
                     /pseudo
     CDS             8337..8420
                     /locus_tag="VYO70_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            8750..9655
                     /locus_tag="VYO70_001615"
     CDS             8750..9655
                     /locus_tag="VYO70_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1713821.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            9652..10467
                     /locus_tag="VYO70_001616"
     CDS             9652..10467
                     /locus_tag="VYO70_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1713822.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWISAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
BASE COUNT         3158 a         2227 c         2060 g         3151 t
ORIGIN      
        1 atttgacaat cgatagccgt cgcttaatcg aaagatggaa aaaagaaggg aaatcaaata
       61 gagaaattgc ctctctgctt ggaaaagttc ctcaaactat ccacactgaa atcaagcgtg
      121 ggacagtccg acaatgtctt ggaaaagggc gcttcaaaga ggtttattct gccgactatg
      181 ctcaacagtc ttatgaaaac aatcgcaagc gctcggtcag gaaatcaagc gtgaccaagg
      241 aactaaagga aaagattctc cactatcata accaaaaatt ttcgcctgaa atgatggtga
      301 tggttaaagg ggttaacgtg ggaatttcaa ccatttacta ttggattcat catggaaaat
      361 tggggttaag caaacaggat ctgctttacc ctagaaaagg aaaagcgctt aagaaacagg
      421 ctagcaccaa ctttaaacct gctggtcaat ccatcgaaca gcggtctgaa gctatcaatc
      481 ttcgcttgga gaatgggcat tatgagattg atacggttct acttacgaga gcgaaaaact
      541 actgtttgct tgtcttgact gatcgaaaga gtagacatca gatcatccga ttgattccaa
      601 ataaaagtgc tgatgtggtc aatcaggctc taaaactcat cttaaaacaa cacaagattc
      661 tttccatcac ggcagataat ggaacggaat tcaatcgctt gtttgatgta ttttctgagg
      721 agcacatcta ttatgcgcac ccctatgcct cttgggaaag gggaactaat gagaatcaca
      781 acaggctcat tcgtagatgg ttacctaagg gaatcaagaa aatgactccc aaagaagtcg
      841 cattcatcga aaagtggatt aacaactatc ctaaaaaatg cttggactac aagtcaccca
      901 gagaagactt ctggatggct aacttgaact tgaaatttag cgtcagaaac aaatcacgaa
      961 attgattcaa attttgcagg tcgtttaaat atcctgcgtg cgggtgttct tgatgctaac
     1021 gatggaatta tttccattgc tggtgtggtt atcggagttg ccagtgccac gagcaatatc
     1081 tggattatct ttttatcagg ttttgcggct atcttagctg gtgccttttc aatggctggt
     1141 ggagaatatg tatccgtttc aactccaaaa gataccgagg aagctgccgt ttcgcgagaa
     1201 aaactcttgc tagaccaaga tagggaacta gccaaaaaat ccctctatgc tgcttatatc
     1261 caaaatggag aatgcaaaac ttctgcccaa ctcttgacca ataagatctt tcttaaaaat
     1321 ccactcaagg ctctggtaga ggaaaaatat gggattgagt atgaagaatt taccaatcct
     1381 tggcacgctg ccatttctag cttcgttgcc tttttcctta gaagtttgcc tccaatgctg
     1441 tcagtgacca tattcccaag tgaatatcgc atccctgcta ctgtccttat tgtcggtgtg
     1501 gcccttcttc tcactggtta cactagtgcc agacttggaa aggatccgac tagaacagct
     1561 atgattcgga acctagctat ggtctcttga ctatgggagt taccttcctg ctcgaacaac
     1621 ttttcagcat ttagaataca agaaatacct cgattttgaa gtcgaggtat cttttttaca
     1681 tttgcacaat cttacgataa cttcttgaag taatcatgaa aatcagcaca taggcgatga
     1741 ggaagatagc gcagatagac aaggtcacga tcaacatcat agtcgtatcc agtacaccaa
     1801 tcacttttaa aatcaggcta agcatatggt aggcaaaggc tagatgtatg aaggcaaaaa
     1861 gcaaaggaag gaagaaaaca gttaaaacct gtttgttgat ggtttgcttg atttgctttt
     1921 gatctaaacc gactttctgc aagataataa agcgttcacg gtcttcatag ccttcagaaa
     1981 tttgtttgta gtagatgacc agaacggttc cgaccataaa gataatggat aggaaaatac
     2041 caataaagaa gacaccgcca aagaggacac tcatttgagc actagcatct gctagagtgc
     2101 taccatacac atagttacct tcagtgttta attgagcatt aaacttttgt aagtattttt
     2161 catattcttc agcgaccttg agttgctctt cttcactgat acttacattc ataccaccgt
     2221 aaaactgatt atagatatcc gaatctggga attgatccaa aaaggcttgt aaatcaggaa
     2281 caacaaggta attgtaatca gtagtcaaga tattaaactt atttggaaca tggttcacaa
     2341 tgaaatcttt attaaattct tctttgacag aaaattgatg atcatttaga gttagagctt
     2401 tctgtccttt cagtccgtca tttttggcaa agagaccgac ctcatttcct gatagagaca
     2461 gtttttgacc agtcatattt tcataatctt tttggtcaaa taccatgaaa actgttttgg
     2521 gttggacacg gttttgtcct ttttcaaaaa tagttaactt ggttccttct tgatttgcaa
     2581 taccaaagtt actgtaacga agtacttctt tctctttgac actataacct ttgtcacttg
     2641 caaactggct caagagtttg tccaaatctt ctttttcaac attttgccct gaaaccccaa
     2701 aatcatgagg atttagaact tttttaaagc tttctgcgga attgaaaatg cttgtcgctg
     2761 ctgacatggt taccaaaacc attgttgaca aaatagcgat ggttgctagt ccaaccgcat
     2821 ttttcttcat acggaaaatc aagttggaaa cagatatgag gttattaggt tggtaatagt
     2881 atttcttgtt tttctttaag atttgtagga agactgtaat ccctgcatta aacaagagat
     2941 aggtcccaaa gataaccagt aaaacagcta tgaagaaggt tgttaaggct gtaagaggat
     3001 cttttaccgt aagggcaaga taatagccaa tccctaaact tatggaacca agaatagttt
     3061 ggagaggtag gaaacgacct cttttctctc cgcttgcttt ctcacgcgag agctggaggg
     3121 cattcatacg ggcgattcga agggcattca ggaacatgag gcctaggaaa atcaatccaa
     3181 agacaagaag tactgcaatg acaactttca tctggaaggt agcaaccagc tcaaccttca
     3241 atttcattag tttgagcagg aaagcgaaaa ttaacttgtc aaacaaggct ccaataccga
     3301 tacccgctcc aacagttaga atcccaaata ccactaactc cttaaaggtc atactgataa
     3361 gatgacgctt ctccaagccc aacatgccat aaattcctag ttccttggaa cggttcttca
     3421 tgacaaaact attggcatag agaacgataa tggctgacgc aagggtgacg acaaacatac
     3481 caaatccaag tgtagcctga atggttgttc ctccacgaat ttcagcaatc ttgggattga
     3541 aggttagaga gtaaaagaga taggtgacag tgactgccaa gagaacagct agcgcaaaag
     3601 gatagtagag tttgcggttt ttaatcaagt tcgataccgc taacttatta gttaatcgaa
     3661 acatactaat tcacctcgct tgccatgaca gtcaaggtat cagagatttc ttggaacatc
     3721 tgacgttctg tcttctctcc acggtagatt tggttgtaaa gaatgccgtc tttgataaag
     3781 agaacacgct tggccctgct agcagctgct gttgagtggg ttaccatgag gatggtttgg
     3841 ccgcgctcat tgatttcatc aaagacatca agtaaggctg cagatgactt ggagtcaaga
     3901 gctcctgtcg gttcgtccgc aaggagaatt tcaggttcag tgatgatggc gcgtgctact
     3961 gctacacgct gtttctggcc accagaaatc tcgtaagggt acttctcttg taattggttt
     4021 attcccagat tctcagctgt caccaccaat ttcttcatca tctctgtaat aggtcttctt
     4081 gacaagacaa gcggaagcaa gatattgtcc tttacagata gagtatctag caagttaaag
     4141 tcttggaaga caaagcccaa cttttcacga cggaagctag aggcctgtga atttttaatg
     4201 gttgcggtgt cagttccatt caagtaaacc tgaccacgac ttggtttatc cagcatagct
     4261 agaatattga gaagagttga tttaccagaa ccagactcac ccatgatggc aacgtagtca
     4321 cccttttcga cggtaaagtg aatatccttg agggcctcta cttggttgcc ctgaaaacga
     4381 gttttataaa ttttttgaac gtgttttaca tctaaaagtg tcatgagaat ctcctttctt
     4441 aatatcccat caaatgaaat gtcgcttgca tcatagcgca tcccagctga acgcgctcga
     4501 tttctttgtg acttggtttt cttgttttct tatgtaagat ttgtttcatg atgttactcc
     4561 tttgttcttt atgagtctag tttacatcaa aaaaagacct cttgccataa cctaacctta
     4621 caaaagagac tttaatctta catttttgta agattggggg aaatgtaagc aaaattactt
     4681 agaaaatgat ttgatttcct atttttaaaa ttgtaaaata taaatatgaa aaggcttact
     4741 ttggaaagga ggaagcggga actaacattt tagtaagctt acctagataa cataccattc
     4801 aatttaacac ttgcaagtcg ttcaacctat aaggaaagaa ggtgcgcctt aatgaaacac
     4861 aaagaacata tactcatcgg acttctctat ctgctctctc cattcatcgg tcagctctta
     4921 gttgaacaca cacactttat cagtacagaa tttaccggca ctgcttatgt tatttgctgg
     4981 ttatcagttg ttatcagcat ccaccatttt tccaaaaatg tattgtctca acagcaaaaa
     5041 tagattaaaa aactgcgttt aaattactgc ttttatagta aaatgaaata agaacaggac
     5101 aaatcgatca ggacagtcaa atcgatttct aacaattttt tagaagtata ggtgtcctat
     5161 tctagttcca atccactata gtttgtggat gaagctggcg aaatattatt aatactattc
     5221 aaaaatcaaa gtgcaaacta tatcaacttc acattgccga ctaaattaca gcctgcggct
     5281 ggcttcctag tttgcacttt gatttttatt gagtataaaa gtaaaaaagc tttaaatcaa
     5341 gcagtttaga ctattgattt aaagcttttt gcgtattcca aatcttttgt aaaacctcac
     5401 caaaaccagc cttcattatc gtcaatggct tgagcttctt tgcgtttacg tgggcctgtt
     5461 ccgtacattt ctgcttcgat ttcttccttg gttggcatga tagaagctag gatgatatag
     5521 ataatcaccc caagtccaaa gtttgcgact gtaaaaatgg caaacagaaa acgtacaagg
     5581 gtaacatcaa agttccactt gtctgacaga cctgctaaaa ctcctgaaat catgcgattt
     5641 cgtctcattt tataaaactt actgttcatg atatttcctc tttctgctag gttagacata
     5701 gtatatcacg gataggctcg gctttcatca gtcctcaggc tgatttacta atagcaactt
     5761 tcctcgacaa agtccacagc gatagcgttt ggtatcaatc ctacgcttac gctgataagt
     5821 ttgctggcag gattggcaac gatagagctt gattggcttg gagttactat tgggcaagga
     5881 tggtacaaac cgtaatccat ccactgcttt caacagttcc ttaaaatccc gatccttgtg
     5941 ttgatagcct ttcccttgaa aatagaggtg ataatgacag agttcatgtc ggacaatttt
     6001 cctaaaaacg tccaacccca gttcctgata aaccttggga ttaaaatcca aatgcccatc
     6061 tttggggaaa aatcgcccac ctgtcgaacg tagacgccta ttccactgga catgatggat
     6121 aaaaggtctg ccgaagtctt ctagtgaaac ctgcttgacg taatcagtca gtttcatttg
     6181 gagctaggag agacagatta actttttcac gttcagtatc aattttctta acccaaacgc
     6241 tcaccaaatc tccaactgcc accacttgac tagggtgttt gataaacttg cgactcatat
     6301 gggaaatatg gatgagaccg tcctcatgaa ttccgatatc aacaaaagca ccgaaatcaa
     6361 caacgttacg caccactcct tctagctttt gtccaaccac taagtccttg atatctagga
     6421 catcttgacg gagaactgga gcatcaaagg aatcacggaa atctcgacct ggtttgagaa
     6481 gatctgcaat gatatcttta agagtttctg gaccaaggtc taactcttgc gccatttcct
     6541 tgactgaaag cgacttgagt ttgctttggg cctcttcatt cagatcctta atgtccaagc
     6601 atttgaagag ttccttaacg gcagcgtaat tttctgggtg aactcctgta ttatcaagga
     6661 tattgctact ttcagggata cggaggaaac cagcagcctg ttcaaaagcc ttggccccca
     6721 aacgaggaac tttcttgatt tgggagcgtg aagtgatttt tccctcttcc tcacggtatt
     6781 tgacgatatt ttcagaaatg gttttattga gtccagctac gtgtgaaaga agagctgggc
     6841 tagctgtatt gacattgaca ccaacttggt taaccactgt atcgacaaca aagtccagac
     6901 tctcagatag tttcttctga ctgacatcgt gttggtattg accgacacca attgacttag
     6961 gatcgatttt gaccaattcc gcaagaggat cttgcaaacg acgggcgata gaaatggcag
     7021 agcgtttttc aacggtcaag tctggaaact cctgacgagc aagttcgctg gcagaataga
     7081 cagaagcacc actttcatta acgataacat agctgacttc agggaaatct ttcagaactt
     7141 ccgctacaaa agcttcactt tcacgactgg ccgttccatt tccaatggca ataatctcta
     7201 caccgtattg accaattaaa tctgctaaat ctttcttggc ttcttcgatt tgacgagctg
     7261 atgctggttt aacaggataa ataacctgag ttgtcagcat ttttcctgtt gcatccacga
     7321 cagctagctt ggcacctgta cgaaaggctg ggtcaaaccc aagaaccacg cgccctttca
     7381 gtggagcaac caagaggaga ttgcgcagat tgtcagaaaa aagttggata gctccctctt
     7441 cagctttctc agttaattct gtccgaatac gacgctcaat agcaggcaag acctttttct
     7501 taacggattg ctgaacaact tcatcaatat aagcattttt caccttgaaa cgagtaacaa
     7561 agaaggcaag aatacggtcc gtcgcatgtt caaaaccgat cttcaagaca ccaagtttct
     7621 ccccacgatt gagagccaag gtacgatagc cttgcatagt tccaactgtc tctgaaaaat
     7681 cataataaat ctgaaaaacc tgcttttcat caagactttc atccttggct tgagaagtga
     7741 gtttagagtg tctcagcact tcctgataag tcatagaacg caaggtcaca tcttccgata
     7801 aggcttcgac caaaatatca actgcaccgg tcaaggcttc cttgccagtc gcaaatcctt
     7861 cacagacgaa cttttcagct tctttctcta agtcaactat attctgcaaa atcaagcgag
     7921 caagaggaaa gagtccagct tcacgggcaa tggttgcctt ggtacgacgc ttttccttat
     7981 aaggaagata gagttcttca acgtctgcta atttttcggc aactaagata gcttcttcca
     8041 attccttggt caacttgcct tgctcttgaa tcttagctaa gacagcttcc ttacggtcat
     8101 tgagatttgt cagactttta tccaaatcaa taatagcctt aatcgccacc tcatccagac
     8161 taccagtcat gtccttacga taacgcgcga taaagggaat agtcgcccct tcagctgtca
     8221 aacttagaac ggtatcaatt tgctttaacg tcactcccaa atcctgagag attttttcat
     8281 attttttatc cataaatcta ttataccaca agctggaagg ctttctccaa tccttttcat
     8341 actcaatgaa aatcaaaaag caaactagga agctagccgc aggctgctca aaatactgtt
     8401 ttgaggttgt ggataagact gacgaagtca gctcaaaaca ctgttttgag gttgtggata
     8461 gaactgacga agtcagtaac cgtactacgg taaggcgaag ctgacgtggt ttgaagagat
     8521 tttcgaagtg tatcaactat cgaaaacaaa ttaaaaaact cccttttccg caaaataata
     8581 gtataataga ggtagaattt agaatcgagg tacacctatg gctgtcaaat ttacaaaacg
     8641 agacgacttg gacaagatgt ttgaagagtt tgctaaactc cctgatttga aacaagttac
     8701 tttccctgat gacaaagaga aaaaagtcaa agcagaaaag aaaaactaga tgactgcttt
     8761 tcaacaactc ccatctagtg tacttcaaac tggagccatt tttctctcca ttatcattga
     8821 agcccttccc ttcgttctga taggaagcat tgtctcaggg ctgattgaag tttatatcac
     8881 acctgacaag gtttatcatt ttctccctcg aaatcgttgg gggagaatct tttttgggac
     8941 ctttgtcggt atacttttcc cttcttgtga atgtggaatc gtccccatca tcaatcgttt
     9001 tctggaaaaa aaggttccaa gttacacggc cgttcctttt cttgtgacag cacctgttat
     9061 caatcccatt gttctttttg cgacctattc tgcctttggc aactccttcc atgtcgccct
     9121 attacgagct ctgggttcca ttcttgtggc tgtaatacta ggaatttttc taggattttt
     9181 ctggcaagaa ccgattcaga aagaaaatcg tctggcttgt catgagcatg atttttctta
     9241 cttgagttct gcaaaaaaag tttttcaagt ctttgtgcag gccattgatg aattttttga
     9301 tacggggcgt tatttggtat ttggctgcct ctttgcttct ataatacagg tctacgttcc
     9361 gactcggatt ctgacctcta tcagtgcgac tcctcttttt gccatcctgc tcttgatgct
     9421 tttggccttt cttctttcgc tctgtagtga ggcggatgcc tttataggtg cttctctact
     9481 ctcgagtttc ggtttggctc cagttctggc ctttctcgtc attggcccaa tgctggatat
     9541 caaaaatatt ctcatgatga aaaattactt gaaagcacga tttattagtc acttcattac
     9601 gattgtgact ctggtcgttt tagcctattc tctcttgatt ggagtcatcc tatgattcga
     9661 tttttagttt tagctggcta ttttgaactg actatttacc tccatctgtc gggcaaacta
     9721 aaccagtaca tcaacatgca ctattcctat ctggcctata tttccatggt gctttccttt
     9781 atcttggcaa ttgttcaatt gtatatctgg atgaagcaag tcaaaaccca cagtcatctg
     9841 aacagccgat tggccaagat gacgagtatt tctcttctgg ctattccact tgtcatcggc
     9901 ttaactttcc caactgttag cttggattct cagactgttt ctgctaaagg ttatcatttc
     9961 cccctatcgg aaggaacgga tctagccatt cagacaagcg aagggacgac aagccaatat
    10021 ttgaaaccag ataccagttc ttatttttca aaatcagcct atgaaaagga aatgcgaacg
    10081 gcggcggata aatacttatc ccaagatagt attcagatca ctaatgaaaa ctatatggaa
    10141 gtcatggagg ctatctacga ctatccagat gagtttgagg gcaagacaat ccagtttaca
    10201 ggctttgtct ataacgaccc cagtcatgcc aatagtcaat ttctgttccg attcggcatt
    10261 atccactgta tcgcagattc tggtgtctat ggattgctga ccaagggcaa tacccggcag
    10321 tatgaaaaca acacttggat ttcagccaaa ggaaaactgg tcaatcacta ccataaagaa
    10381 ctcaaacaaa accttccaac cttggaaatc gacagcttta ccaaagtcga taaaccagaa
    10441 aatccctatg tatatagagc tttttaagaa aatcaaaata aaaacgaaca agttctcttc
    10501 tgaataacag aaaaaggctc tataatattt gtagtgggta aatcccctat agatattatg
    10561 gagcttattt ttgtgtagaa aaaaagtccc atatga
//
LOCUS       DAVLYV010000067        10505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLYV010000067 DAVLYV010000000
VERSION     DAVLYV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.067"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..422
                     /locus_tag="VYO70_001617"
                     /pseudo
     CDS             1..422
                     /locus_tag="VYO70_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            460..762
                     /locus_tag="VYO70_001618"
     CDS             460..762
                     /locus_tag="VYO70_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1713823.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSVPVFDNIRLPAKQNMV"
     gene            770..1572
                     /locus_tag="VYO70_001619"
                     /pseudo
     CDS             770..1572
                     /locus_tag="VYO70_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1621..2085
                     /locus_tag="VYO70_001620"
     CDS             1621..2085
                     /locus_tag="VYO70_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1713824.1"
                     /translation="MGGHPGFNCPLLDDEVYEDYYLEFEKEETCSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDRGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2242..2667
                     /gene="lacA"
                     /locus_tag="VYO70_001621"
     CDS             2242..2667
                     /gene="lacA"
                     /locus_tag="VYO70_001621"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1713825.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2698..3213
                     /gene="lacB"
                     /locus_tag="VYO70_001622"
     CDS             2698..3213
                     /gene="lacB"
                     /locus_tag="VYO70_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1713826.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPTEENKKLIAKIEHVESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3224..4153
                     /locus_tag="VYO70_001623"
     CDS             3224..4153
                     /locus_tag="VYO70_001623"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1713827.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIKGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQEFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            4155..5135
                     /gene="lacD"
                     /locus_tag="VYO70_001624"
     CDS             4155..5135
                     /gene="lacD"
                     /locus_tag="VYO70_001624"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1713828.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5369..6205
                     /locus_tag="VYO70_001625"
     CDS             5369..6205
                     /locus_tag="VYO70_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1713829.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLITENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6241..6558
                     /locus_tag="VYO70_001626"
     CDS             6241..6558
                     /locus_tag="VYO70_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1713830.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6560..8254
                     /locus_tag="VYO70_001627"
     CDS             6560..8254
                     /locus_tag="VYO70_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1713831.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERKGNSSSELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            8469..9875
                     /gene="lacG"
                     /locus_tag="VYO70_001628"
     CDS             8469..9875
                     /gene="lacG"
                     /locus_tag="VYO70_001628"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1713832.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGHYSEKTLAGVEAILAANGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDVIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9918..10175
                     /locus_tag="VYO70_001629"
     CDS             9918..10175
                     /locus_tag="VYO70_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1713833.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDELEEFKNLFLNFINPEEWDTISFDSDFMPFQQS"
BASE COUNT         3380 a         1798 c         2163 g         3164 t
ORIGIN      
        1 atcaagaata actctttgat caggtagcaa gcttgttgga ggaacgagaa attgtaactc
       61 caacttatcg tgaagccttg atcacgcgtg aaaagtcatt tccaactggt ttagatatgg
      121 aatttctagg aaaggacttg ccaaatgtag cgattcctca cacagatatt gttcataatt
      181 tggctgaaaa agttgtggtc gttcgattag agaaaccggt gactttccat aatatgattg
      241 cgccagataa ggaagtagaa gtatccttgc tcttctttat cattaataat tcaagttcaa
      301 gtcaaacgaa cattcttgct cagttaatgg acttcttcac aggaaatggt catcttgaag
      361 acctatcaaa aatttctgaa ccagaagccc tttatgctta cattgctgaa gcaaccgctt
      421 aatcttgtct attaaaaaat aaaatcggag gaaatccaaa tgattaaaat tcttgctgcc
      481 tgcggtgcag gtgttaactc aagtcaccaa attaaaagtg ctctagaaga agaactttca
      541 aaccgtggtt acgatgttca ctgtgatgca gttatggtga aagatgtaaa cgaagacctt
      601 atgaaaggtt acgatatctt tacaccaatc gctgcaacag accttggttt tgaaccaggt
      661 atcccagtta tcgaagctgg gccaatctta ttccgtatcc cagcaatgag cgttccagta
      721 tttgacaata ttagacttcc tgcgaaacaa aacatggtat agtagttcta tgaatgatga
      781 agcaagtaaa caactaactg atgcacgatt taagcgtctt gttggtgttc agcgcacgac
      841 ttttgaagag atgttagctg tattaaaaac agcttatcaa cttaaacacg caaaaggtgg
      901 acgaaaacct aaattaagcc tagaagacct tcttatggcc actcttcaat atgtgcgaga
      961 atatcgaact tatgaagaaa ttgcggctga ttttggtatc cacgaaagca acttactccg
     1021 tcggagctaa tgggttgaag taactcttgt tcaaagtggt tttacgattt caagaactcc
     1081 tctcagttct gaggacacgg taatgattga tgcgacggaa gtacaaatca atcgccctaa
     1141 aaaagaatta gcgaatgatt ctggtaaaaa gaaatgccac gctatgaagg ctcaagcgat
     1201 tgtcacaagt caagggagat tgtttctttg gatatcactg tgaactattg tcatgatatg
     1261 aagttgttca aaatgagtcg cagaaatatc ggacaagctg gtaaaatctt ggctgacagt
     1321 ggttatcaag ggctcatgaa gatatatcct caagtacaaa ctccacgtaa atccagcaaa
     1381 ctcaagccgc taacagttga agataaaacc tataaccatg cgctatctaa ggagagaagc
     1441 aaggttgaga acatctttgc caaagtaaaa acgtttaaaa tgatttcaac aacctatcga
     1501 aatcatcgta aacgcttcgg attacgaatg aatttgattg ctggcattat caatcatgaa
     1561 ctaggattct agttttgcag gaagtctaat aaagaaacta gcaaggtcag gccttacttt
     1621 attggtggac atccaggatt taattgtcct ctacttgatg atgaagtcta tgaagattat
     1681 tatctagagt ttgagaaaga agagacttgc tctgttccac gtcctttccc agaaacaggt
     1741 atgttggatt tccaagatag aagtccatgg ctagagggac aaaaagaaat agatcttagt
     1801 tatgatcttt tcagcaaaga tgcagtgact ttggatgaat tgcaatctcg aacaattgcc
     1861 cttcgttctc ttaaacatga taggggattg aaagtgcatt ttgcagaatt tccaaacctc
     1921 atcatctggt caactttgaa caagggacct ttcattacct ttgaaccatg gtctggcttg
     1981 tcaacattcc ttgaagaagg agatcattta gaagataaga agaatgtttg tctcttagaa
     2041 gccaatcagg ttgaagaatt agggtttgag atagaagttt tataaaaaag taaaaaataa
     2101 ataacaaaaa caaacacaaa ctgttgactt cttcaaacaa tagtagtata ttatgtttgt
     2161 gaggactaat tgactgtttt ttaaagacag ccaatacact gctggaatcc agcatagaaa
     2221 aaataaaaag gagtatacaa tatgtctatt gttatcggtg cagatgctgc aggtttgaga
     2281 ttgaaagaag ttgtgaaaga cttcttagaa aaagaaaact tccaccttgt ggatgttaca
     2341 gctgaaggtc aagactttgt tgatgtgact ctcgctgttg ctgcagaagt aaacaaagaa
     2401 gaacaaaacc ttggtatcgt gattgatgct tatggagctg gtccatttat ggttgcaact
     2461 aagatcaaag gaatggttgc tgcagaagtt tctgatgaac gttcagctta tatgactcgt
     2521 ggccacaaca actcacgtat gatcactatg ggtgcacaac ttgttggtga tgaattggct
     2581 aaaaacatcg ctaaaggatt tgttaatggt aaatacgacg gtggtcgtca ccaaatccgg
     2641 gttgacatgt tgaacaaaat gggctaatta gattatagta agaaaggtaa gttaaaaatg
     2701 agaattgcaa ttggatgtga ccacatcgta acagatgaaa aaatggcggt ttcagaattt
     2761 ttgaaatcaa aaggatatga agtcattgac tttggtacat atgaccatac acggactcac
     2821 tacccaatct ttggtaaaaa agtaggggaa gctgtaacta gcggtcaagc tgatcttgga
     2881 gtatgtatct gtggtactgg tgttggtatc aacaacgctg taaataaagt tccaggtgtt
     2941 cgttctgcct tggttcgtga tatgacaaca gccctttatg ctaaagaaca attgaacgct
     3001 aacgttattg gttttggtgg taaaattact ggtgaattgc ttatgtgtga tatcatcgaa
     3061 gctttcatcc atgctgaata caaaccaact gaagaaaaca aaaaattgat tgcgaaaatt
     3121 gaacatgttg aaagtcacaa tgctcaacaa acagacgcaa acttctttac agaattcctt
     3181 gagaaatggg atcgtggaga ataccacgac taagaggtga cctatgattt taacagtcac
     3241 aatgaaccca tccatcgata tttcctatcc cttggatgag ttgaagattg atactgtcaa
     3301 tcgtgtggtg gatgtaacca aaacggctgg tggtaaggga ctcaatgtta cccgagtact
     3361 ttcagaattt ggcgattctg ttcttgctac tggtttagtg ggtggcaaac ttggtgagtt
     3421 tttggttgaa catatcgata atcaagtaaa gaaagatttc ttctcaatta agggagaaac
     3481 tcgtaactgt atcgctattc tccacggaga caaccaaaca gaagttcttg aaaaaggtcc
     3541 tgaagtattg gaacaggaag gtcaagagtt tttggaacat ttcaaaaaac tcttggagtc
     3601 agttgaagta gtggccatct caggtagtct gccagctggc cttccagttg attactatgc
     3661 gagcttggta gaacttgcta atcaagctgg caagcctgta gtcttggact gctcaggtgc
     3721 agcacttcag gctgttcttg aatcacccca taaaccaaca gtcatcaaac caaataatga
     3781 agaattgtct cagcttcttg gaagagaagt ttctgaggat ttggatgaat taaaagaagt
     3841 acttcaagaa cctttgtttg cagggattga atggattatc gtttcacttg gtgccaacgg
     3901 tacttttgcc aaacatggtg acactttcta caaggtagat attcctagaa ttcaggtggt
     3961 aaatcctgtt ggatctggag actctactgt ggcaggaatt tcttcaggac ttcttcacaa
     4021 agaatcggat gcagaattac tcatcaaggc aaatgtcctt ggtatgctca atgctcaaga
     4081 aaaaatgact ggtcatgtca acatggccaa ctatcaagtt ctatatgatc aattaatagt
     4141 aaaagaggta taaaatggct ttaacagaac aaaaacgtgt acgcttagaa aaactttctg
     4201 atgaaaatgg tatcatctca gctcttgcat ttgaccaacg tggtgctttg aaacgcctca
     4261 tggctcaaca ccaaacagaa gaaccaactg tggcccaaat ggaagaactt aaagtcttgg
     4321 tagcagatga attgactaaa tacgcttcat ctatgcttct tgaccctgag tatggacttc
     4381 cagcaactaa agctcttgat gaaaaagctg gccttctcct tgcttatgaa aaaacaggtt
     4441 atgacacaac aagcacaaaa cgcttgccag actgcttgga tgtctggtct gcaaaacgta
     4501 ttaaagaaga aggtgcagat gcagttaaat tcttgcttta ctatgatgta gatagctcag
     4561 acgaactcaa tcaagaaaaa caagcctaca tcgaacgcat cggttctgag tgtgtggctg
     4621 aagatatccc attcttcctt gaaattcttg cttacgatga aaaaattgcg gatgcaggtt
     4681 ctgtagaata cgcgaaagta aaaccacaca aagttatcgg cgctatgaaa gtcttttcag
     4741 acccacgctt taacattgat gtcttgaaag ttgaagttcc tgttaacatt aaatatgttg
     4801 aaggcttcgc tgaaggtgaa gtagtttata cacgtgaaga agcagcagcc ttcttcaaag
     4861 cgcaagatga agcaacgaac ttgccataca tctacttgag tgctggtgta tcagctaaac
     4921 tcttccaaga tactcttgta tttgctcatg aatcaggtgc aaactttaac ggagttcttt
     4981 gtggccgtgc tacatgggca ggatcagttg aagcttacat caaagatggt gaagcagcag
     5041 ctcgcgaatg gcttcgcaca actggatttg aaaacattga tgagctcaat aaagttcttc
     5101 aaacaacagc gacttcatgg aaagaacgtg tgtaagaaag tcctcctagt ttaggaacat
     5161 gaatctaaaa aaatttaaaa aaagttgtat gtaaaggttt acaaaataac ttacttgtgc
     5221 tatacttaaa tcacaagtta atacaaggtg agtgttacta agtaatatta ggcatgatca
     5281 caggtgaatt agaaatcagc tgattttcta gttcatttgt ggtcattttt tgtactcata
     5341 tacctttaag atataaaagg aggttgacat gtatcgaatt ctaaatccaa tgaatcacaa
     5401 tgtctcgctt gtcagaaatg ataagggaga agaggtgatt gtaattggta aggggattgc
     5461 attcggaaag aagaaggggg atttgattac tgaaaatcag gttgagaaaa tctttcggat
     5521 gaagaccgaa gagtccagag aaaactttat ggctcttctc aaagatgttc cgcttgattt
     5581 tatcacagtg acctatgaaa tcattgataa gctatcaaag aaatatcatt atccgattca
     5641 agagtatctc tatgtaacct tgacagatca tatttactgt tcttatcaag ctctaactca
     5701 aggaaggtac aaggatagta atctgccaga tatttccgct aagtatcctg tcgcttttca
     5761 aatcgcaaat gaagcttttg aaatttaccg tcagaagcta gcagatcatt ttcctgagga
     5821 cgaaattatt cggattgctt atcatttcat taatgctgaa ggtgaaaatg aagtggaact
     5881 tgtggagtcg attgataaga ggaaagaaat tctcaggaat gttgaagaag ttttaacgga
     5941 ctatgcaatt caacgaacta aaaagaataa ccatttctat gatcgcttta tgatccattt
     6001 gaattatttc ttggattatt tagacagatc tagagatgat aaccaatcac ttctggatat
     6061 ggaagatcat attaaacaat cctatccaaa agccttcgag attggttcca agatctatga
     6121 tgtgattacg caacatacgg gtcttgattt gtataaaagt gaacgagttt atctagttct
     6181 acatatccaa cgtttattgt cataaaaatt tatttaaaac tatataagga gaattctatc
     6241 atgaatagag aagaagtaac attgttaggt tttgaaatcg tagcctatgc tggcgatgct
     6301 cgttcaaaac tattggaagc cttgaaggct gctgaagctg gtgattttga aaaagcggac
     6361 gctctggtag aggaagctgg tagctgtatt gcagaggctc accacgcgca aacaagtcta
     6421 ttgactaagg aagcttctgg tgaggactta gcgtatagtg tgaccatgat gcatggtcaa
     6481 gatcacttga tgacaactat cttgttaaaa gacctaatgc accatttaat tgaactttac
     6541 aagagaggag ttaaataaaa tggataagtt aataattttt attgagaaag gaaagccttt
     6601 ctttgaaaaa ttatcgagaa atatatactt aagagcgata aaagatggtt ttatttccag
     6661 tatgccagca gtattattct caagtatttt tatactaata gctgctgtgc ccaatatttt
     6721 tgggtttaaa tggtctgatg aacagttagc ttttattttg aaaccttaca actactcaat
     6781 gggaatttta gctctcttag tagctgggac aactgcaaaa tcgttaacag attcagttaa
     6841 tacacgtagt atggagaaaa ccaaccaaat caactacatg tcaacttttt tagcagctgt
     6901 tgttggattg ttaatactgg cagctgatcc aattgaaggt ggatttgcta atggtctttt
     6961 aggtactaga ggattactta ctgcattttt ggcagctttt attacagtaa atatctataa
     7021 agtatgtatt aaaaataatg taaccattag acttcctgaa gaggttccac caaatattgc
     7081 acaagttttt aaagatgtta ttccgtttgc cttatctgtt ctttcaatat atggattgga
     7141 tttaattgtt agaaatattt ttggtacaaa tgtagcagaa tctgttggta aaatattagc
     7201 ccctctattt tcagctactg atggttatat cggtttagct attgtatttg gtgcttatgc
     7261 tttcttttgg tttgttggaa ttcatgggcc atctgttgta gaacctttaa ttgtagctat
     7321 cagttatgca aatatagaag ctaatgttca gcttgtgcaa gctggtatgc acgcagataa
     7381 aattttaaat ccagttactc aaacttttgt tgtaacaatg ggtggtactg gtgcaacact
     7441 agtagttcca tttatgttta tgtggttatg taaatcaaaa agaaatagaa ttgtcggacg
     7501 tgcgtctgta gttcctactt tctttggcgt aaatgaacca atcctatttg gtgctccaat
     7561 tgttttaaat cctatcttct ttattccgtt tgttactgct cctattatca atgtttggat
     7621 tatgaaattt tttgtagatg ttctacaaat gaatagtttc agtattattt taccttggac
     7681 aacacctgct ccaattggta ttgttatggg aacggcgctg gctccattgt cattcgtctt
     7741 ggctataact ttaatcatta tcgacacttt aatctattat ccatttgtta aagtttatga
     7801 ccatcaaatt ttagaagaag aacggaaagg aaactcttca tctgaattga aagaaaaagt
     7861 tgctgcaaac ttcaacactg caaaagcgga tgctattctt gaaaaagcgg gtgtcgatgc
     7921 agcacaaaat accatcactg aagaaacaaa tgtcctcgtt ctctgtgctg gtggaggtac
     7981 aagtggactc cttgcaaatg ctttgaataa ggcagcagca gaatacaatg tccctgtgaa
     8041 agcagcagca ggcggctatg gtgctcaccg tgaaatgtta ccagagttta atctagttat
     8101 ccttgcccct caagttgctt caaactttga agatatgaag gcagaaacag ataagctcgg
     8161 tattaaactt gctaagacag aaggcgctca atacatcaaa ttaactcgtg atggaaaagg
     8221 tgccctagca ttcgtacaag agcaattcga ttaaggctag gaactgtgaa acagtctccc
     8281 atcgttacgg aaatcgctat ggcgaatttc ctaatcgcct tattaattcg tcggtaaaaa
     8341 gatatcgttt ttacctcctc atgtcacaat tcagtgactt ggtacaagaa gtgagatgga
     8401 gaagcatggc tcactgactc ctctcctctc acttttactt tatttaaatc aagaaatagg
     8461 tgaaaaaaat gacaaaaaca cttccaaaag actttatttt cggtggcgca acagctgctt
     8521 atcaagcaga aggtgctaca catactgatg gaaaaggacc agttgcttgg gataaatatc
     8581 ttgaggataa ctactggtac actgccgaac cagctagtga tttttacaat cgatatccag
     8641 ttgacctcaa gctagcagaa gagtatggtg tcaatggtat tcgaatttct attgcttggt
     8701 cacgtatttt cccgactggt tacggccaag taaatgctaa aggtgttgag ttttatcata
     8761 atttatttgc agagtgtcac aaacgtcatg ttgagccttt tgtaactctt catcactttg
     8821 acacgccaga agctctccac tcaaatggag acttcttaaa ccgtgaaaat atcgaacatt
     8881 ttgtagacta cgctgctttc tgttttgaag aatttccaga agtaaactat tggacaacct
     8941 ttaatgaaat tggaccaatc ggtgatggtc aatatttggt tgggaaattc cctccaggta
     9001 tccagtacga ccttgccaaa gtctttcaat cacaccacaa tatgatggtg tctcatgcac
     9061 gcgcggtaaa attgtacaaa gagaaaggct ataaagggga aattggtgtt gttcacgccc
     9121 tgcctactaa atatcctcta gatcatgaaa atccagcaga tgttcgtgca gctgagttgg
     9181 aagatattat tcacaacaaa tttattttgg atgcaaccta tctgggtcac tactctgaaa
     9241 agactctggc aggggtagag gctatcttgg ctgccaatgg tggtagcttg gatcttcgtg
     9301 aagaagattt tacagcatta gaagctgcaa aagacttgaa tgatttccta ggaatcaact
     9361 actatatgag tgactggatg gaagcctttg atggagaaac tgaaattatc cataatggta
     9421 aaggtgaaaa aggaagctct aagtatcaaa tcaaaggtgt tggtcgtcgt gtagctcctg
     9481 actatgtacc acgcacggat tgggattgga ttatctaccc tcaaggtttg tatgaccaaa
     9541 tcatgcgcgt gaagaaagat tatcctaact acaagaagat ttacatcact gaaaatggtc
     9601 tcggctataa agatgagttc gttgataaca ctgtttacga tgatggtcgt attgattacg
     9661 tgaaacaaca cttggagatc ttgtctgatg tgattgcaga tggagctaat gtaaaaggtt
     9721 acttcatttg gtcattaatg gatgtcttct catggtcaaa cggttatgag aaacgttatg
     9781 gtctcttcta cgtagatttt gaaactcaag aacgttatcc taagaaatca gctcactggt
     9841 acaagaaagt agcggaaact cagattatag actagtagaa ttagtcatta gatatagaat
     9901 tttagtgagt caaaaagatg ttcaaagatt ttatccaatc tatttatgaa aaagtttata
     9961 ttataaattt cgaaaaatgc tctcaaatac cgtgtttgac gagtgaagaa ttgaaaagtc
    10021 ttggaaaatg gtatgtctcg actggtaaag aatggatttg tcattcagat gatgagctgg
    10081 aagaatttaa aaatctattt ttaaatttta tcaatcctga agaatgggat actatctcct
    10141 ttgattcaga ttttatgccg tttcaacaat cgtaaccaat ttctcaaaaa agttaaatct
    10201 tatatttagt actctgtaaa actcttatct aatcacgttg cttatactca atgaaaatca
    10261 aagagcaact ttaaactagg aagcgagtcg cagatttctc aatgcatagc tttgaggaat
    10321 tgggcaaaaa gtctttgata tagaaaaacg catagtatca ggtgtttcaa cacctgatac
    10381 tatgcgtttt attgtgggaa gatttacttt tttcttctga aattgagttg ttacccaggc
    10441 tctttcagtt tattaaggtt tgatgacttt aatgtgttta gatagcttaa aaaggattga
    10501 atcac
//
LOCUS       DAVLYV010000068        10459 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLYV010000068 DAVLYV010000000
VERSION     DAVLYV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10459
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.068"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..160
                     /locus_tag="VYO70_001630"
     CDS             <1..160
                     /locus_tag="VYO70_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1713834.1"
                     /translation="FAISSKGEKMKVENISYRVDHRKLFDNISFDTSSSDVTLITGKN
                     GTGKSTLL"
     gene            complement(526..1836)
                     /locus_tag="VYO70_001631"
     CDS             complement(526..1836)
                     /locus_tag="VYO70_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1713835.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCSLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2073..2945
                     /locus_tag="VYO70_001632"
     CDS             2073..2945
                     /locus_tag="VYO70_001632"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1713836.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
     gene            3076..3922
                     /locus_tag="VYO70_001633"
                     /pseudo
     CDS             3076..3922
                     /locus_tag="VYO70_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            3983..4945
                     /locus_tag="VYO70_001634"
     CDS             3983..4945
                     /locus_tag="VYO70_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1713837.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            5122..6129
                     /gene="gap"
                     /locus_tag="VYO70_001635"
     CDS             5122..6129
                     /gene="gap"
                     /locus_tag="VYO70_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1713838.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(6197..7033)
                     /locus_tag="VYO70_001636"
     CDS             complement(6197..7033)
                     /locus_tag="VYO70_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1713839.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWKEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLAHCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            7159..9354
                     /locus_tag="VYO70_001637"
     CDS             7159..9354
                     /locus_tag="VYO70_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1713840.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETKAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEASWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            9407..9559
                     /gene="rpmG"
                     /locus_tag="VYO70_001638"
     CDS             9407..9559
                     /gene="rpmG"
                     /locus_tag="VYO70_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1713841.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNLKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            9569..9745
                     /gene="secE"
                     /locus_tag="VYO70_001639"
     CDS             9569..9745
                     /gene="secE"
                     /locus_tag="VYO70_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1713842.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            9800..10336
                     /gene="nusG"
                     /locus_tag="VYO70_001640"
     CDS             9800..10336
                     /gene="nusG"
                     /locus_tag="VYO70_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1713843.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         3274 a         1810 c         2276 g         3099 t
ORIGIN      
        1 ttttgctatt agttcaaaag gagaaaaaat gaaagtagaa aatatttcgt atagggtgga
       61 tcatcgtaaa ttgtttgata atatttcttt tgatacttcg agttcagacg tgacattaat
      121 tactggtaaa aatggtacag gaaagtcaac tttactatag tagattgaaa ctagaatagt
      181 acacatctac ttctaaaata ttgttagaaa tcgatttgac tatcctgatc tatttgtcct
      241 gttcttattt catttcacta tatctcaaat tgagtatgac gaagtgcgct cccatgtcct
      301 gggaacgcac tttcttcata tttttcatat tcttgaatcc atcgataaag actattggga
      361 tgaattttta aagttgaact aatcattttt acaggatgag atttacagca gagagtttga
      421 aggctttatc aaaggttttt cttggcataa tgacttttcc tcgtttccac ttaattttgt
      481 gtctacttta ttataccaag tccaccctta agttagataa taaatctaac ttaaggaagc
      541 tagaaggatg agaatccagg tggtcaagag tcccaaactt aagctgatgg ggacacccag
      601 aataatttgc tttttgaagg caaggccacg ttcctctata ttgggaagtg agagttgaat
      661 gagagaacca gctgatgaaa agggtgagat attagtagat agagcgccaa taacggtggc
      721 tgttgtgagt aagtgaatat caatctgagg attttgagca ctgatgatag caatgatggg
      781 aaagagggct ggagctacaa cggatagggt ggaactaaag agtgacatca ctccggctat
      841 cacacaaaag aacagaggta accagaaatg aggaatggtt gttgtcatga ggtgccctat
      901 cagtgtgact aaacctgact tgaccgctag agacattagt aagctcatgc cgcagagcat
      961 gataattgta gcccagggaa ccttagctaa aatggcttct tgcttcccta atttgagcct
     1021 taaggcgagg cagaccatga gtattgagac aaagccaata tcaaatgttt tttgataagt
     1081 agctatccag gcgatgtttg ggaaaatgag atgcaacaag ggaaaaagcc aaaccaaaac
     1141 catgctgctg atcatgagca aggtggtttg tctttgaacc ttgctgagga gtggtggttg
     1201 gtcaatagtc aaggatgagt ttgttcttcc cttactatag tgactgtaac aggataataa
     1261 aagcaagacg atgagtgggt agataatgct gacgataaag atatgattgc caagtgaaaa
     1321 agcttgctct tcccatccca tttgcttaaa caggccttga aagacaatgc ctgagctact
     1381 ggttatcaaa ttagcccctc ctgaagctcc ccaattgacg gcttgagctc caatcaaagg
     1441 gtgtttgtcc gctttttgac agagggtaat cgctagagaa cagcaaacgg ccatagtagt
     1501 gaaaaatcca gcacctaaag cagacaaaag ggttgccatc aggtataaaa tcatgtagag
     1561 ggcgttaggg tgggtgcgtg tgcggtagag aatgtgttga gccaaaacat caagagtacc
     1621 gttagttgtt gcaacgttat aaaagagaga gacgctaaaa atggtaaaaa agagtgaggt
     1681 tggccaaaaa tgaagaagtt ctttggggct taatcccatg agagtggttg cgatgaggta
     1741 agaaaaagca atagccagca ggccaatatt gattttggtg cggtaaccaa ttccaatggc
     1801 tagagcaatg gcgctaatca ttattaaatg aatcattgaa ttgtcctttc agttagaata
     1861 tggaaatagg ttggcaattt tggcgctcaa aagaccacct gtttcctatt ggtacttgac
     1921 tattatacac aaaaaaaata caattgtctt gacaattgta ttgacacttg ttagaattaa
     1981 aaatgaggta ttcaggcttg tgatttccta tggaagttaa tagtgattgc ctctaatgct
     2041 tacaagtgat attaaaaata gaggacctag tgatgtcaat catttcaact gatttaaccc
     2101 cttttcaaat agatgataca ttgaaagcag ccttgcgaga agatgttcat tccgaagatt
     2161 acagtaccaa tgccattttt gatcatcatg gccaagccaa ggtgtcgctt tttgccaagg
     2221 aagctggtgt tttagcgggg ctaaccgttt ttcaaagggt ttttacccta tttgatgccg
     2281 aggtgacctt ccagaatcct catcaattta aggatgggga tcgtttgact agtggcgatt
     2341 tggttttaga aatcataggc tcggtgagaa gtctcttaac atgtgaacgc gttgccttga
     2401 attttttaca acatttatca gggattgctt cgatgacagc tgcttatgta gaagccttag
     2461 gcgatgattg cattaaggta tttgatactc gaaaaactac tcctaattta cgtctttttg
     2521 agaaatatgc cgtgagagtt ggcggtggct ataatcatcg ctttaattta tcagatgcta
     2581 tcctgctaaa agacaatcac attgcggcag taggtagtgt tcaaaaggca attgctcaag
     2641 cgcgtgccta tgcccctttt gtgaaaatgg tcgaggtgga agtggaaagc cttgctgctg
     2701 ccgaagaagc tgcggcggcg ggtgctgata ttatcatgtt ggataatatg tcattggaac
     2761 agattgaaca ggccattacc ctaattgcag gacgttctcg gattgaatgt tctggaaata
     2821 ttgatatgac cactattagc cgttttcgtg gtttagcgat tgattacgtc tccagtggta
     2881 gtttaaccca tagtgctaag agtcttgatt tttccatgaa ggggttaacc taccttgatg
     2941 tctaagttgt aaaataaact aactttttaa aggatgtctt tcctctagaa cgagttttat
     3001 gtcagatagt ttaaacgcct cttcaaatat agtaaaatga accaaaaata gtacacaatg
     3061 tggtataatc tttttatggc atattcaata gattttcgta aaaaagttct ctcttattgt
     3121 gagcgaacag gtagtataac agaagcatca cacgttttcc aaatctcacg taataccatt
     3181 tatggctggt taaagctaaa agagaaaaca ggagagctaa accaccaagt aaaaggaata
     3241 aaaccaagaa aggttgatag agatagactt aaaaactatc ttactgacaa tccagacgct
     3301 tatttgactg aaatagcttc tgaatttggc tgtcatccaa ctaccatcca ctatgcgctc
     3361 aaagctatgg gctacactcg aaaaaaagaa ccacacctac tatgaacaag acccagaaaa
     3421 agtagcctta tttcttaaga attttaatag tttaaagcac ctaacacctg tttagattga
     3481 cgaaacagga ttcgatactt atttttatcg agaatatggt cgctcattaa aaggtcagtt
     3541 aataagaggc aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac
     3601 aaatggtgag ttaatcgctc caatgactta cgaagagatg atgacgagcg acttttttga
     3661 agtatggttt cagaagtttt tcttaccaac attaaccaca ccatcggtta ttattatgga
     3721 taatgcaaga ttccatagaa tgggtaagct agaactcttg tgtgaagagt ttgggcataa
     3781 acttttacct cttcctccct actcacctga gtacaatcct attgagaaaa catgggctca
     3841 tatcaaaaag cacctcaaaa aggtattacc aagttgcaat accttttacg aggctttttt
     3901 atcctgctct tgtttcaatt gactatatat cacggataca gttgggaaag aagctattaa
     3961 atatttggag agtccttttg ggatgaacat gtcaaatatt actatagatg ggcaagaaat
     4021 ggatctgagt caaacgatgc cttcttatgc tcaagtagtg agtctttatg atgatacaag
     4081 tatctctgtt agtaataatg agacggacaa agtgttggct ggatccctct atactgatac
     4141 aaatgaacag ggattaacga ttccaagcag tttactaaaa aattggaatg aacagacagg
     4201 aaaaaatttg acagctaatg atcttattgg caaatcagtc tcagccagca ttgtagaaag
     4261 tgctgccgaa actagtaaga ttgctcaatt tcaaacgaag attgtacgtg taatcaatga
     4321 tgaagatgac atggaggaca gcaacagttt catgctgtct catcaaatgg aaacgatttt
     4381 gaaagaggct ggatttacga aagctgtatc ttattttatc ttggaactca aagatccatc
     4441 acagacaaaa gtagtaacag aagaattaca gaaaaataag aagtatactg tgctttctca
     4501 acagagagtt cttgatattg tgattacctt tattcgtgtt attcagggat tattgattgt
     4561 gctttcatca caagctattg tggtagcagc ggttatgatt ggtatcatta tttacatcaa
     4621 tatcatgcaa cgttccaagg aaataggtgt catgaaagca gttggttatc agaatcgtga
     4681 tgtcaaagga atttttattt acgaggctat ctggattgta ggcatcgcct tgctgctggc
     4741 atttttggtt gcacaagggg tgggaagttt ggcgaatgcg attgtaagtc acttttaccc
     4801 atccatcact aaggtttttg aattaaatct tttatctgtt ttaggaactc tagttttcgc
     4861 tctattactt ggttatgtct cagcctactt cccggcgcgt aagattagta aaatggatcc
     4921 tatagaatcg ttatgctatg aatagtaagg gattccttgg tttacagtct taaaaagaaa
     4981 ctaacataga aaaacaatgg ccatacttgc aattcaactc aaatagttgt ataataggtt
     5041 tgttgaaagg tgatttgtag tgattcaagt tactcttttc acaataaaaa tttcatgatt
     5101 ttcataagga ggaaatcact aatggtagtt aaagttggta ttaacggttt cggacgtatc
     5161 ggtcgtcttg ctttccgtcg tatccaaaac gtagaaggtg ttgaagttac acgcatcaac
     5221 gaccttacag atccagttat gcttgcacac ttgttgaaat acgacacaac tcaaggtcgt
     5281 ttcgacggta ctgttgaagt taaagaaggt ggatttgaag ttaacggtaa attcatcaaa
     5341 gtttctgctg aacgtgatcc agaacaaatc gactgggcta ctgacggtgt agaaatcgtt
     5401 cttgaagcta ctggtttctt tgctaagaaa gaagcagctg aaaaacacct taaaggtgga
     5461 gctaaaaaag ttgttatcac tgctcctggt ggaaacgacg ttaaaacagt tgtattcaac
     5521 actaaccacg acgttcttga cggtactgaa acagttatct caggtgcttc atgtactaca
     5581 aactgcttgg ctccaatggc taaagctctt caagacaact ttggtgttgt tgaaggattg
     5641 atgactacta tccacgctta cactggtgac caaatgatcc ttgacggacc acaccgtggt
     5701 ggtgaccttc gccgtgctcg cgctggtgct gcaaacatcg ttcctaactc aactggtgct
     5761 gcaaaagcta tcggtcttgt aatcccagaa ttgaatggta aacttgacgg atctgcacaa
     5821 cgcgttccaa ctccaactgg atcagttact gaattggtag cagttcttga aaagaacgtt
     5881 actgttgatg aagtgaacgc agctatgaaa gcagcttcaa acgaatcata cggttacaca
     5941 gaagatccaa tcgtatcttc agatatcgta ggtatgtctt acggttcatt gtttgacgca
     6001 actcaaacta aagttcttga cgttgacggt aaacaattgg ttaaagttgt atcatggtac
     6061 gacaacgaaa tgtcatacac tgcacaactt gttcgtactc ttgaatactt cgcaaaaatt
     6121 gctaaataat tcttgagttg atagaaagca aggctttgtg gtcttgcttt tttatatgga
     6181 aaaatggatg acacgatcat ccattctttt ttaattcttt ttcaaatgta tttgaaaggg
     6241 tagtgaaagt tagcttctct aaagtaagtg ggtgggtaaa ggaaagtcgg aaggcatgaa
     6301 gcataagccg gcttgtcttt gatttactat tatagagagg gtctcccagg ataggaagat
     6361 tatgatgcga aaggtgcaca cgaatctgat gggttcgccc tgtctttagc ttgcaatgag
     6421 ccaaggaagt cttgtttgag aattgcttta atctgcttac atgcgtttca gcatattgcc
     6481 cattttttgc atcaactatt ctttttctac gatcatggcg atcacgtcca attttgtctc
     6541 tgaaaacaag ttcttttctg ttgatatttc catcaactag agcccaatat tctctagaaa
     6601 tctctttttt ctccaataag cgattgagaa tgggcaggat aaaaggattt ttggcaaaga
     6661 gaactaagcc actggtttcc atgtccagac gatgaacgac atagcaggtt tggccaacat
     6721 aggtactgac atggttaaga agggcaattt cgtttggttg attaccatgc gttttcatcc
     6781 cctctggttt gtttacaata atcaagtgtt gatcttgata aacttcctgc actaagtctg
     6841 ggttgcccca agggatcgtc ttttgggaat aatcttcctc gtcaaaagtc aactggcaaa
     6901 catctccagg atttacgatt tccttccagt ggacttcttc ttgatttatc aaaatatgtt
     6961 tcttgattct caaaaaatga cggatttttc tagggatgag gagttgttcc tcaagtaatt
     7021 gctttactgt catttgaggt agagaggcgg gtaatgtaaa tgtgaattgc atacagatat
     7081 tgtaacaaaa aaagccctat ttggatagga aatagctaaa ttcttgtctt cctatgatga
     7141 agatgataaa ataaacgcat gaaattagat aaattatttg agaaatttct ttctcttttt
     7201 aaaaaagaaa caagtgaact agaggactct gattctacta tcttacgtcg ctctcgtagt
     7261 gatcgaaaaa aattagccca agtaggtccg attcgaaaat tctggcgtcg ttatcatcta
     7321 acaaagatta tccttatact aggtttgagt gcaggcttgc tagttggaat ctatttgttt
     7381 gctgtagcca agtcgaccaa tgtcaatgat ttgcaaaatg ccttgaaaac tcggactctt
     7441 atttttgacc gtgaagaaaa agaggctggt gccttgtctg gtcaaaaggg aacctatgtt
     7501 gagctgactg acatcagtaa aaacttgcag aatgctgtta ttgcgacaga agaccgttct
     7561 ttctataaaa atgacgggat taactatggc cgttttttct tggctattgt cactgctgga
     7621 cgttcaggtg gtggctctac cattacccaa cagctggcta aaaacgccta tttatcgcag
     7681 gatcaaactg ttgagagaaa agcgaaagaa tttttccttg ccttagaatt aagcaaaaaa
     7741 tatagtaagg agcaaattct aaccatgtac cttaacaacg cttattttgg aaatggtgtg
     7801 tggggtgtag aagatgcgag taagaaatac tttggagttt ctgcatcaga agtgagtctg
     7861 gatcaagctg cgactctggc agggatgctc aaggggccgg aactgtataa tcccttgaat
     7921 tccgtagaag attctactaa tcggcgcgat actgtcttgc agaatatggt tgcagcagga
     7981 tatattgata aaaaccaaga aaccaaagct gctgaagttg atatgacttc gcaattgcac
     8041 gataagtatg aaggaaaaat ctcagattac cgttacccct cttattttga tgcggtggtt
     8101 aatgaagctg tttccaagta taatctaaca gaggaagaga ttgtcaataa tggctaccgc
     8161 atttacacag agctggacca aaactaccaa gcaaatatgc agattgttta tgaaaacaca
     8221 tcgctatttc cgagggcaga ggatggaacg tttgctcaat caggaagtgt agccctcgaa
     8281 ccgaaaacag ggggagttcg tggagttgtc ggtcaagttg ctgacaatga taaaactgga
     8341 ttccggaatt tcaactatgc aacccaatca aagcgtagtc ctggttctac aattaagcct
     8401 ttagttgttt atacgccagc agttgaagca agctgggctt tgaataagca gttggataac
     8461 cataccatgc agtatgacag ctataaggtt gataactatg cagggatcaa aacgagtcga
     8521 gaagttccta tgtatcaagc cttggcagaa tcgcttaatc tacctgctgt tgccactgtt
     8581 aatgatttgg gtgtcgacaa ggcttttgag gcaggcgaaa aattcggact caacatggaa
     8641 aaggtcgacc gtgttcttgg tgtcgccttg ggaagcggtg ttgaaaccaa ccctcttcaa
     8701 atggctcaag catacgccgc ctttgcaaat gaaggtttaa tgcctgaagc tcattttatt
     8761 agtagaattg aaaatgctag tggacaagtt attgcgagtc ataaaaattc acaaaaacgg
     8821 gtgattgata agtctgtagc tgacaagatg accagtatga tgttggggac tttcaccaac
     8881 ggtaccggta ttagttcatc gcctgcagac tatgtcatgg cagggaaaac tggaacaact
     8941 gaagcagttt tcaatccgga gtacacaagt gaccagtggg taattggtta tactccggat
     9001 gtagtgatta gccactggct tggctttccg accactgatg aaaaccacta tctagctggc
     9061 tctacttcaa acggtgcagc tcatgtcttt agaaacattg ccaataccat tttaccttat
     9121 acgccaggaa gtacctttac ggttgaaaat gcttataagc aaaatggaat tgcaccagcc
     9181 aatacaaaaa gacaagtaca aaccaatgat aatagccaga cagatgataa tttgtctgat
     9241 attcgagggc gtgcgcaaag tctagtagat gaggctagcc gggctatctc agatgcgaag
     9301 attaaggaaa aggctcaaac aatatgggat tcgatagtca atctatttcg ctaagatgct
     9361 tgtcaaagcc tagctttctt gttataatgg ataagatgga ggcgttatgg cactaaaaaa
     9421 agcaagccta gcttgtgcgg tttgtggttc gagaaactat tcaatcaaga tcagcggaaa
     9481 cctcaagcct acacgactag aagtaaataa attttgtaag cattgtggca agtacactac
     9541 acacagagaa acgagatagg agagagcgat gcgttttatt ggagatattt ttagacttct
     9601 taaagacaca acatggccaa ctcgcaagga aagctggaga gattttcgtt ctatcatgga
     9661 atacacagct ttctttgtag taattattta catttttgac cagttgattg tttcaggttt
     9721 gattcgattt attaacattt tttagaagat tagtggagtt aattacacta gaaatcttct
     9781 atttatgaaa ggaaatatca tggatagttt tgataaaggg tggtttgttt tacaaactta
     9841 ttctggttat gaaaataagg tgaaagaaaa tctattacaa cgtgcacaaa cctacaatat
     9901 gttggataat attctacgcg ttgaaattcc aacacaaaca gtgcaagttg aaaaaaatgg
     9961 aaagagaaaa gaagtagaag aaaatcgctt tccaggttat gttcttgtag aaatggtcat
    10021 gacagatgaa gcttggtttg ttgttcgaaa cacaccaaat gttacaggat ttgtcggatc
    10081 acacgggaac agatcaaaac caactccatt attggaacaa gaaattcgcg acattttggt
    10141 atctatggga caaactgttc aagaatttga tttcgatgtt gagattggtc aaactgtacg
    10201 tattattgat ggtgcttttg cagactacac tggtaagatt acagaaattg ataataacaa
    10261 agtgaaaatg attatctcta tgtttggtaa tgacacagtt gcagaagtaa atctaaacca
    10321 aatcgcagaa ttataaccct gagagaggct ggagcctctc ttttttgtgc agtttaggag
    10381 ctaaagggaa cagaatggag aaaatggaac aaatgtgttt tctaatctgt tagactgtat
    10441 ctagaaaggg gaaaattat
//
LOCUS       DAVLYV010000069        10414 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLYV010000069 DAVLYV010000000
VERSION     DAVLYV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.069"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            228..431
                     /locus_tag="VYO70_001641"
     CDS             228..431
                     /locus_tag="VYO70_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713844.1"
                     /translation="MNTEQLNQALQMTIREMSTTSTDSMIASNFLSIQLNEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            434..784
                     /locus_tag="VYO70_001642"
     CDS             434..784
                     /locus_tag="VYO70_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713845.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQLKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTGEPKE"
     gene            794..1210
                     /locus_tag="VYO70_001643"
     CDS             794..1210
                     /locus_tag="VYO70_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEV1713846.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLFRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            1214..1546
                     /locus_tag="VYO70_001644"
     CDS             1214..1546
                     /locus_tag="VYO70_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1713847.1"
                     /translation="MQQITEIIIAFATSFLTVAVGGIVKAVKDYLLRKGGEKAVIIAE
                     ILAKNAVHAVEQVASETGYKGEEKLEQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGR"
     gene            1550..2506
                     /locus_tag="VYO70_001645"
     CDS             1550..2506
                     /locus_tag="VYO70_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1713848.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTDDLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKQDIENGLSAATGWQKNGTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLSD
                     RWKKHTDGNWYYFDQSGEMATGWKKIAEKWYYFDVEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            complement(2644..2823)
                     /locus_tag="VYO70_001646"
     CDS             complement(2644..2823)
                     /locus_tag="VYO70_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713849.1"
                     /translation="MRKVIQELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(2965..3114)
                     /locus_tag="VYO70_001647"
     CDS             complement(2965..3114)
                     /locus_tag="VYO70_001647"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713850.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
     gene            3395..3862
                     /locus_tag="VYO70_001648"
     CDS             3395..3862
                     /locus_tag="VYO70_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1713851.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            3895..3992
                     /gene="ffs"
                     /locus_tag="VYO70_001649"
     ncRNA           3895..3992
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYO70_001649"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            4049..4492
                     /locus_tag="VYO70_001650"
     CDS             4049..4492
                     /locus_tag="VYO70_001650"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1713852.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            4494..5009
                     /locus_tag="VYO70_001651"
     CDS             4494..5009
                     /locus_tag="VYO70_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1713853.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            5023..6384
                     /gene="radA"
                     /locus_tag="VYO70_001652"
     CDS             5023..6384
                     /gene="radA"
                     /locus_tag="VYO70_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1713854.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSTNVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            6457..6954
                     /locus_tag="VYO70_001653"
     CDS             6457..6954
                     /locus_tag="VYO70_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1713855.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            6979..7794
                     /locus_tag="VYO70_001654"
     CDS             6979..7794
                     /locus_tag="VYO70_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV1713856.1"
                     /translation="MKKSSILFIFILLLCIDLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAYKGQKVGKKQGLI
                     FLGLALGTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            7939..8907
                     /locus_tag="VYO70_001655"
     CDS             7939..8907
                     /locus_tag="VYO70_001655"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1713857.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(9041..9322)
                     /locus_tag="VYO70_001656"
                     /pseudo
     CDS             complement(9041..9322)
                     /locus_tag="VYO70_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9449..9682)
                     /locus_tag="VYO70_001657"
     CDS             complement(9449..9682)
                     /locus_tag="VYO70_001657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713858.1"
                     /translation="MFNQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFTFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
     gene            complement(9675..9791)
                     /locus_tag="VYO70_001658"
     CDS             complement(9675..9791)
                     /locus_tag="VYO70_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1713859.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(10006..10356)
                     /locus_tag="VYO70_001659"
     CDS             complement(10006..10356)
                     /locus_tag="VYO70_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1713860.1"
                     /translation="MLRHPGISPTNDLIAKKIFSNPEITCQFIRDMLDLPAKNVTILE
                     GSDIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQVN
                     QNLEKIRQREGDTH"
BASE COUNT         2980 a         1880 c         2431 g         3123 t
ORIGIN      
        1 aattgtctca cgagatcctg agaatccaga tggctatctg catattgact ataccgcttt
       61 agttccttac ttaatcaagg ctattcaaga attaaatcaa aaaatagaaa aaatggagaa
      121 aacaatagca tgaataacaa catgttgacc aatatcgcac tttaaagcaa ttcaggagct
      181 tgctcttgaa aatagaaaac gaacacacag attggagaac ttagaaaatg aacacagaac
      241 agcttaacca agccttacaa atgacaattc gtgaaatgtc aacaacttca acagattcga
      301 tgattgcaag taatttcttg agtattcagt taaatgagca aagggaagag aatcaaagac
      361 ttcaagcacg agtggatgag ctggaagctc tgcttgatga acaaactaaa ccagcagaca
      421 aaggagaata gacatggcag aaacaattca aaacacagat aacttactag accttacaaa
      481 aatcacagaa ccatttgatc ttgcgagtgc tttgcgctac atgaaagaaa atggagagtt
      541 cattcgttgc aagaatgtaa gcgatgactt ctatatgtat cgtgatgttc aaaaacgtcc
      601 tgtgatcgta aatggccgtc gccaactcaa ggatgttgaa accgtttggg cgttcaacca
      661 gtggggtggt acaatcgcaa caatcaacgt agccgttctg ttgaatcatg aattctatat
      721 catgaaattt gatgcagagg gcaatcctga ctggacggat ccaacgggag aacctaaaga
      781 ataggagatg tgtatgcaaa ttgaattttt caattttcta agaagtgtcg tacagactga
      841 agatggtttg gtcttgtacg ctctagcact gattgtctca atggaaatca ttgattttgt
      901 gacagggacg attgcggcga ttatcaatcc tgacatcgag tacaagagca aaatcggcat
      961 caatgggcta tttcgtaaga tttcaggggt tctcttactg atgatcctca ttccggcgtc
     1021 cgttttgttg cctgaaaaga caggttttgc attcttgtac tcaatctatc tcgggtacat
     1081 cgcatttact tttcaatctc tcattgaaaa ttaccgcaaa ttaaaaggaa atgttactct
     1141 ttttcagccg attgtaaaag tatttcagcg attacttgaa aaagatgatg acacgaaaaa
     1201 aggagaataa caaatgcaac aaattactga aatcatcatt gcttttgcga caagcttttt
     1261 aacagtagca gtaggcggta ttgtaaaagc agtaaaagat tatcttttgc gtaaaggcgg
     1321 agagaaagcg gtgatcatcg ctgaaattct agctaaaaat gcagttcatg ccgttgagca
     1381 agtagcttca gagactggct ataagggcga agaaaagctg gagcaggctc gtgctaaagt
     1441 ccgtgctgag cttacaaaat acaatattag catgactgac aaagacttag acaccttcgt
     1501 agagtcagca gtgaagcaga tgaatgacgc atggaaagga cgatagggaa tggatatcga
     1561 tagaaacaga ctacgtacag gcttgccaca ggttggggtg cagccttatc gacaagtaca
     1621 tgctcactca acaggtaacc gcaactcaac cgtacagaat gaagcggatt atcactatag
     1681 aaaggaccct gaactagggt tcttttcaca tgttgtcgga aacggccgtg tcatgcaagt
     1741 aggtcctgtg aacaacggaa gttgggatgt tgggggcggt tggaatactg agagttacgc
     1801 agcggttgaa ctgattgaaa gccattcaac taaagaagag ttcatgacgg actaccgcct
     1861 ctatatcgaa ttgctacgca atctagcgga cgaagcaggc ttgccgaaga ctcttgatac
     1921 agacgacttg gcaggtatca agacgcatga atactgtacc aataaccaac caaacaacca
     1981 ctcagaccat gtggatccat atccatatct tgctaaatgg ggcattagcc gtgaacagtt
     2041 taagcaagac atcgaaaacg gcttgagcgc tgcaacaggc tggcagaaaa atggcactgg
     2101 ctactggtac gtacactcag acggctctta tccaaaagat aagtttgaga aaatcaacgg
     2161 tacctggtat tatttcgatg gctcaggcta tatgctttca gaccgctgga agaagcacac
     2221 agacggtaat tggtactact ttgaccaatc aggcgaaatg gctacaggct ggaagaaaat
     2281 cgctgagaag tggtactatt ttgatgtaga aggtgccatg aagacaggct gggtcaagta
     2341 caaggacact tggtactact tagacgctaa agaaggcgcc atggtatcaa atgcctttat
     2401 ccagtcagcg gacggaacag gctggtacta cctcaaacca gacggaacac tggcagacaa
     2461 gccagagttc acagtagagc cagatggctt gattacagtt aaataaatag aaaggaaact
     2521 ttctaaattg ttctttcacc gcaggcttag gcttgcggtt ttttatttgc tctgattctt
     2581 taaaaaagcg ttttcttgaa gaatagagag gtgttttgtc aaaaataaaa acagtgaccg
     2641 aaatcactgc ttatcagttg tagcaaattc ataaagtttt tctgctgtga gaagtgccat
     2701 tttgtccatg cttgtttttc cttttctgag gtcagaaaca gtagtccatg gaactccagc
     2761 accttgggaa atagcagatg tagacatcga actgtctaac aattcttgaa taacttttct
     2821 catcttattt gtccttttta ttttttagat aaatatatac attgattgca attataaaaa
     2881 tagctattgc actaaccatt gcatttcctc tttccatttg ataaaataga ggtgtaaggg
     2941 gctttcgccc caacctctta gcgtttacct ttttctttga cgggatttcg gtttacgctt
     3001 tttgttttgc cttgcgaccg ttattgcggt cactagactt gcgatagcag ttaccgtttc
     3061 aggaatattg tctattgcct tttcaagtaa cctgagccaa tcttctttgt tcaacttcat
     3121 cacccccttt ccttatcttg attatattat atcacggtac accgagaaag tcaagcgttt
     3181 tgatgaagtt tttttaattt tttcaaaaaa aaatagacct tgtccagagg tcggggagtt
     3241 ggagggggca ccctccaaaa gcattgattt aataagattt tattttacct ttttcataat
     3301 aatctcccta tagagtcacc gcattcggtg gctttttttg tgttgggatt catgatataa
     3361 taataaaatc gataagtagg aaaagagaaa agagatgtat tatacgcttg aagaaaaaga
     3421 agtctttatg agggaggctt tgagagaggc tgagattgct cttgaacacg atgaaattcc
     3481 aattggttgt gtgattgtca aagatgggga aatcattggt cgtgggcata atgcgcgtga
     3541 ggaattacag cgagcggtta tgcatgcgga aattatggct atagaggatg cgaacttgag
     3601 tgaggagagc tggcgcttgc tggattgcac actttttgtg accattgaac cttgtgtcat
     3661 gtgtagtggg gcgattgggc ttgcccgcat tccaaatgtg gtctatgggg ctaaaaacca
     3721 gaaatttggc gctgctggga gtttgtacga tatcttgaca gatgagcgtc tcaatcatcg
     3781 tgtggaggtt gaaataggaa ttttggaaga tgaatgcgca gctatcatgc aggatttttt
     3841 tagaaataga cggaaaaaat aattttgctt ttaaaatgaa taggaatgtg atataataaa
     3901 tagtggagca acagttctgc gtgaagcggg tcaggggagg aatccagcag ccctaagcga
     3961 tttgaattgt gtgctctttt tttcgtgctt tttccgaata aataagatag aataatctag
     4021 aataaatgat aatagaaaag agaaaattat gaaaattcgt ggttttgaat tggtttcgag
     4081 ttttacagat gaaaatttat tgcccaagcg tgagacagcg catgcggctg gttacgactt
     4141 aaaggttgct gtgcgtacag ttgttgcgcc aggagagatt gtcttggttc cgacaggggt
     4201 taaggcttat atgcagccga ctgaggttct ctacctctat gatcgttctt caaatcctcg
     4261 taagaagggc ttggttttaa ttaactcagt tggggtcatt gatggggatt attatggaaa
     4321 tcctggaaat gaagggcata tttttgcgca gatgaagaat atcacagacc aagaggttgt
     4381 tcttgaagtt ggggagcgta ttgtccaggc tgtttttgct actttcttaa ttgcagatgg
     4441 agatgcagct gatggcgttc gaactggtgg atttggatcg acagggcact agaatgaaga
     4501 ttatctttgt acgtcatggg gagccagatt accgtgagtt agaggagcgt tcttatatag
     4561 gatttgggat agatttggca cccttgtctg agatgggacg gcagcaagtc cagaaattga
     4621 gcaaaaatcc tttactctcg tcagctgaaa taatcgtatc ttctgcagtc acaagagctt
     4681 tagaaacggc ttcgtatgtg gtctgtgcta cgggtcttcc tttaagagta gagcctttat
     4741 tacatgaatg gcaggtctat aaaacaggaa tagaaaactt tgaaacagct agaagactgt
     4801 ttttagaaaa caagggggag ttgcttccta atagtcctat tcaatatgag acagctacgg
     4861 aaatgaagtc tcggtttcta gaatgtatgt ctaagtatcg agaacatcag actgtggtag
     4921 ttgttgctca tcgaatgctc atgcgccagt ttgtgccaaa tgagaagatt gatttttgcc
     4981 aagtgattga gtgtgagtta gagatataga aagaggtttg tcatcgcaaa gaaaaaagcg
     5041 acatttgtat gtcaaaattg tgggtataat tcccctaaat atctgggacg ttgccccaac
     5101 tgtgggtctt ggtcttcttt tgtggaagag gttgaggttg ccgaagttaa gaatgcgcgt
     5161 gtgtccttga caggtgagaa aaccaagccc atgaaactag ctgaggtgac ttccaccaat
     5221 gtcaatcgaa ccaagacgga gatggaggaa ttcaaccgtg tgcttggagg cggagtggta
     5281 ccaggaagtc tcgtccttat cggtggggat cctgggattg ggaaatcaac tcttctccta
     5341 caagtctcaa cccagttgtc ccaagtgggg acagttctct atgtcagtgg ggaggagtct
     5401 gcccagcaga ttaaactacg tgcagagcgc ttaggtgata ttgatagtga gttttatctc
     5461 tatgcagaga ccaatatgca gagtgttcgt gcagaagtgg agcgtatcca gccagacttt
     5521 ctcattattg attccatcca gaccatcatg tctcctgaga tttcaggggt gcaggggtct
     5581 gtttctcagg tgcgtgaagt gaccgctgaa ctcatgcagt tggccaagac caataacatt
     5641 gccatcttta tcgtaggtca tgtgaccaaa gaaggaacct tggctgggcc tcgtatgttg
     5701 gagcatatgg tggatacggt gctttacttt gaaggggagc gtcaccacac ctttcgtatt
     5761 ttgagagcgg tcaaaaatcg ttttggttcc actaatgaga ttgggatttt tgagatgcag
     5821 tcgggcggct tggttgaggt actcaatccg agtcaagttt tcctagaaga gcgtttggat
     5881 ggggcgactg gttcctccat cgttgtaacc atggaaggga cgcgtccgat tttggcggag
     5941 gttcaggctt tggtaacacc gaccatgttt ggaaatgcca agcgtactac gacaggactt
     6001 gattttaacc gtgctagctt gattatggct gttttggaaa aacgggcagg gcttctcttg
     6061 caaaatcagg atgcctatct caaatctgct ggtggtgtta aattggatga acctgcgatt
     6121 gacttggctg ttgcagttgc tattgcttcg agctacaaag acaagccaac taatcctcag
     6181 gaatgttttg tcggagaact gggcttgaca ggagagattc ggcgcgtgaa tcgtattgag
     6241 caacgcatca acgaagctgc taaactgggc tttactaaga tttatgtacc taagaattcc
     6301 ttgacaggaa tcactctgcc taaggaaatt caggtcattg gcgtgacaac gattcaggaa
     6361 gttttgaaaa aggtctttgc ataatccgtg acaaattctc ttaaaaatga taagatagga
     6421 gaaatatttg actatcaaat tttcaaggag ggaatcgtgt cgtattttga acagtttatg
     6481 caagctaatc aggcttatgt tgccctacat gggcagttaa atctgccact taaacccaaa
     6541 acaagagtag ctattgtgac ctgtatggac tctcgtctgc acgttgcgca agctctgggc
     6601 ttggcacttg gggatgctca tatcttgcgg aatgcaggtg gtcgagtgac tgaagacatg
     6661 attcgttcgc tagttatttc ccagcaacaa atggggacaa gagagattgt ggtattgcac
     6721 catacagact gtggtgctca gacctttgaa aatgaacctt ttcaggagta tttaaaagag
     6781 gaattaggtg tagatgtgtc agaccaggac ttcttgccct tccaagatat agaagagagt
     6841 gtacgcgagg atatgcaact gcttatcgag tctcccctaa taccagacga tgtcattatc
     6901 tctggtgcta tttacaatgt tgatacagga agtatgacag tcgtagaatt ataaatactt
     6961 catttagaaa gaaagtgtat gaagaaaagc agtattttat ttatttttat tttattgcta
     7021 tgtattgatt tacagtatga aaccatctac tatacggacg gttcgaggtc aggtgcggaa
     7081 tatggactaa tgggagtttc tatctttcta gctctctttt acatgattcc ggctctttat
     7141 tttctcttcc gtattgggaa aaaatgggaa ttgccaaaga aggttttgat tctgtcttta
     7201 ttgggaggga tgttcctttc aggctggttg tctagttttg ctaatactta tatacatgat
     7261 ttactggggg ttcttttccc agatagtcca tttttaaatg cctttgaaag tgctattgtg
     7321 gctcctttgg tagaagaacc cttgaaatta ttgccacttg tttttgtttt ggctttgatt
     7381 cctgtgcgaa aattaaaatc tttgttttta cttggaattg cttccggttt gggattccaa
     7441 atgattgagg atattggtta tattcgtacg gatttgccag agggctttga ctttactatt
     7501 tcgcgaattt tagagcgtat catctcagga attgcctctc actggacttt ttcaggtcta
     7561 gctgtagtag gtgtttactt gctttacaga gcctataaag gacagaaggt tggcaagaaa
     7621 cagggcctta tttttctagg tttagccttg ggaactcact tcttgtttaa ctctcctttt
     7681 gtggagttgg aaacagagtt gcctttagcg attccagtgg ttacggctat tgctctctat
     7741 ggtttttatc atgcttattg ctttgttgag aaacacaatg agttgatgac ctagaatatt
     7801 tttcaaaaga atgatgcaag ggtacaaata tggtgccctt cttttatttt tgattgaaaa
     7861 atagtgcaaa aagcgctaca atggtagatg gaaaatcttg tgaaaagcac aagttataca
     7921 tatataccgg aggaaatcat gtctttttct gatttaaagc tgtttgccct ttcttctaat
     7981 aaagaattgg cagaacgtgt agcgcaggag attgggatag agttggggaa atcaagtgtt
     8041 cgccaatttt cagatggaga gattcaggtc aacattgaag aatcaatccg tgggaaacac
     8101 gtctttatcc tacaatcaac tagttcgcct gtaaatgaca atctgcttga aattttgatt
     8161 atggtagatg ctttgaagcg tgcgagtgca gaatctgtca atgttgtcat gccttactat
     8221 gggtatgcac gtcaggatag aaaggcgaga gcgcgtgagc caatcacttc aaaacttgtc
     8281 gcaaatatgc ttgaagtagc tggagtggat cgtttattga ccatcgactt gcatgctgcg
     8341 caaattcaag gattctttga tattcctgtg gatcatttga tgggtgctcc tctgattgca
     8401 gattattttg agcgtcgtgg tatggttggt tctgactatg tggttgtcag cccggaccat
     8461 ggaggggtga ctcgtgctcg taagttggca gaatttttga aaacatctat cgctattatt
     8521 gataaacgtc gcagcgttga taagatgaat actagtgaag ttatgaacat catcggtaag
     8581 gttgaaggca agacttgtat cttgattgat gatatgattg ataccgctgg aacgatttgt
     8641 catgcggcag atgctcttgc ggaagctggt gctgttgaag tctatgcaag ctgtacgcac
     8701 ccagttcttt ctggtcctgc tacggacaat atccaaaaat cagctattaa gaaattggtt
     8761 gttttggata ccatctatct gccagaagag cgtttgattg ataagattga gcagatttca
     8821 atcgctcatc tcctagggga tgctatcgta cgtattcatg aaaaacgacc actttctcca
     8881 cttttcgata ttgagaaaaa aatttaatga ccaagcctga gatgattctc aggttttttc
     8941 gtcttttttc cgaataaata gatagtagcg gtgaatctag taaagctaga tttaaaactg
     9001 tgatataata aaaagaaaag aaatatgact gtacgtcatc agaagtttca gcgaccatca
     9061 tttttgaaca gtgatagcac ttgaatcgac gctttctaag gagaattcta gtaggtatac
     9121 cagtcgtttc aagataagga attttagaag gtttttgaaa gtcatatttc ttcaattggt
     9181 ttccgcactc agggcaagat ggggcgtcgt agtccagttt ggcgatgatt tccttgtgtg
     9241 tatccttatt gatgatgttt aaaatctgga cattagggtc tttaatgtct agtaattttg
     9301 tgataaaatg taattgttcc atatgattct ttctaatgag ttgttttgtc gcttttcatt
     9361 ataggtcata tgggactttt tttctacaca aaataggctc cataatatcc ataggggatt
     9421 tacccactat ggatattatg gagccatttt acaacagtgc ctcaaattca gatactgaca
     9481 tacctaattg ctggctggca acctcagaag tcagaagacc ttggcgtact atgtctagga
     9541 aggtaaaaag tttcctttct gcccttcctt caactttccc acgctctaat ccttgttcaa
     9601 ggccttcagc cctagcagtt tccaaggcat agtcctgtgc taacaagact tgttcttctc
     9661 gcatatgtag ttgattaaac attttcctgt cctcctcgga ccagctcttg tagtccagca
     9721 gttgatttgc ttgggtaatg gctcgttgcg gttgctgggt aaagggcttg ttgccgaaaa
     9781 actccaacca tggcttgcga accttgtctt tgctggtttc tctgtatttt ttaattccaa
     9841 gaatgccatc ttaaccagat ggttttcctg tccattgttg gtaatcgcca atacctcacc
     9901 tgttgtgtct tcgcgcatac taaagctatg aaaagccagg tcatctgaga aataattact
     9961 atccacgata gcaatggcgt aaacaggagc gatgtgtttg tagctctagt gagtatcacc
    10021 ttctcgctga cgaatttttt caagattttg attaacctga ctgcacaggt aagcccacaa
    10081 gtgattgatg aaaaaattct gatgatggac ttgaatctca ataattactt gagtaccgtt
    10141 atccaactcc gccaagacgt ctatactggt ataaaaatcc tgcaccgagt aaggcatgga
    10201 gagtaatacg tgaatatcgc ttccctccaa aatggtcaca ttttttgctg gcaagtccag
    10261 catatcgcga ataaattgac aagtgatttc tggattgctg aatattttct tagcaatcaa
    10321 atcattagtt gggctgatgc ccggatgtct gagaatcctc ctattcctcc ttctatagtg
    10381 agttgaaata agatgtgaat aactctatca ggaa
//
LOCUS       DAVLYV010000070        10278 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLYV010000070 DAVLYV010000000
VERSION     DAVLYV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10278
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.070"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..254
                     /locus_tag="VYO70_001660"
     CDS             <1..254
                     /locus_tag="VYO70_001660"
                     /inference="COORDINATES: protein motif:HMM:NF019216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713861.1"
                     /translation="YFKEVKDKATKGLTTFEVNGSSVSSYDDLLTLFKEAVKKDAETL
                     KQEANGNKTVSMNNTVKLKEAVYKKLLQQTDSFKTSIFK"
     gene            315..2036
                     /gene="pabB"
                     /locus_tag="VYO70_001661"
     CDS             315..2036
                     /gene="pabB"
                     /locus_tag="VYO70_001661"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1713862.1"
                     /translation="MHRKTVIDFRTLGERYTFTQPIKELKTRNVEEVADFLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELILEEN"
     gene            2037..2654
                     /locus_tag="VYO70_001662"
     CDS             2037..2654
                     /locus_tag="VYO70_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1713863.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            2704..3462
                     /locus_tag="VYO70_001663"
     CDS             2704..3462
                     /locus_tag="VYO70_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1713864.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLTSDGSYAYSTWQGN
                     YYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNS
                     LFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDD"
     gene            3589..4548
                     /locus_tag="VYO70_001664"
     CDS             3589..4548
                     /locus_tag="VYO70_001664"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1713865.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGNNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPIACTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            4645..5484
                     /locus_tag="VYO70_001665"
     CDS             4645..5484
                     /locus_tag="VYO70_001665"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1713866.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(5532..5702)
                     /locus_tag="VYO70_001666"
     CDS             complement(5532..5702)
                     /locus_tag="VYO70_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1713867.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            5823..6707
                     /gene="miaA"
                     /locus_tag="VYO70_001667"
     CDS             5823..6707
                     /gene="miaA"
                     /locus_tag="VYO70_001667"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1713868.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEASESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            6700..7938
                     /gene="hflX"
                     /locus_tag="VYO70_001668"
     CDS             6700..7938
                     /gene="hflX"
                     /locus_tag="VYO70_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1713869.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     NYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDS
                     EVITGYISEKNKWRLEEFYD"
     gene            7931..8554
                     /locus_tag="VYO70_001669"
     CDS             7931..8554
                     /locus_tag="VYO70_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1713870.1"
                     /translation="MTDIKALALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     DSDGLGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            8569..9498
                     /locus_tag="VYO70_001670"
     CDS             8569..9498
                     /locus_tag="VYO70_001670"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1713871.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKISKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            9519..10274
                     /locus_tag="VYO70_001671"
     CDS             9519..10274
                     /locus_tag="VYO70_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1713872.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLVGETFNYK"
BASE COUNT         3040 a         1988 c         2363 g         2887 t
ORIGIN      
        1 cttacttcaa agaagttaaa gataaagcaa cgaaaggatt aacaacattc gaagtaaatg
       61 gttcttccgt ttcatcatac gatgatttac tgacattgtt taaagaagct gttaaaaaag
      121 atgccgaaac tcttaaacaa gaagcaaacg gtaataaaac agtatctatg aataatacag
      181 ttaaattaaa agaagctgtt tataagaaac ttcttcaaca aacagatagc tttaaaactt
      241 caatctttaa ataattgaaa tctctcatct gctttgcgga tgagggtttt cttattttat
      301 gctatactta aagtatgcat agaaaaacag tgattgattt taggactttg ggggagagat
      361 atacctttac ccagcctatt aaagagttga aaacgagaaa tgtagaagaa gtggcagatt
      421 ttctggcaca agtggaaagc taccaagagc aaggttatta tgtggtgggg tatgtcagct
      481 acgaggctgc acctgctttt gaggaaaaat tagcagttca caaggatccc ctactgggag
      541 agtacctgct ttactttact gttcatgata gcgtagaaac ttcccctatt cctctgactt
      601 atgaggatat tgatttgccc tcaaattggc aggaagtgac gtctgcagca gactatgaaa
      661 aggccattgc ccagatacac catcacttga ggcagggaga cacctatcag gtcaactaca
      721 ccgtccaact caagcaaaag ttaagtgcca atccttttgc catctacaat cgtatggtgg
      781 tagagcagga ggcgggctac aatgcctatg tggaacatga cgagatggca gtgatttcca
      841 tgagcccaga gctctttttt gagcaaaatg atcgcgagtt gacaacacga ccaatgaagg
      901 ggacgactca gcgtggggta actgaccaag aagatcttga acaggccagt tggttggaac
      961 aggatcccaa aaatcgctct gaaaatatga tgattgtgga cctcttgcgc aatgatatga
     1021 accgtatttc tgaagttggg agcgagcacg tggagcgtct gtgccaggta gagcagtatt
     1081 caactgtttg gcagatgact tcgaccatca agagtcagtt gcgagaggat gtggaccttg
     1141 ttgaaatctt ccgctcactc tttccttgtg gttccataac gggtgcaccg aaaattgcga
     1201 caatggagat tatcaaggac ttggagcctc aaccgcgtgg agtctactgt ggaacgattg
     1261 gtctcttgct tccaaatgga cgacggattt ttaatgtggc cattcgtacc attcaacttc
     1321 acaaaggtca agccatttat ggagttggcg gagggattac ttgggatagc acatgggaat
     1381 ctgaataccg agaggttcat caaaaggcag cagttcttta tcgtaaacaa gctcgcttcc
     1441 aactgattac gacaggggaa atcagccaga agaacctgct gtttgaagat caacatctgg
     1501 aaagactgag aaaagctagt cgttattttg cttttccttt tgatgcagaa gacttgggac
     1561 acaagattga ggaagagtgt caggattgtg aagctaatcg agattaccgc ttgcgaatca
     1621 gccttagcaa atctggagag atagaagtca atcgtcaagt attaacccct ctcagtacaa
     1681 gcttttgtca ggcccaagtc tgccttcagg aagctgcttt gaatcaatcc tttacctact
     1741 ttaaaaccac tcaccgaccg catttgagcc taggagaaca agagaagatt taccacaata
     1801 agtcaggaga actgcttgaa acctctatag gaaatttggt tctgaaaatc gctggaaaac
     1861 tctacacacc gcctatccga cttggaatct tgccaggaat ttaccgtcag tatttgctag
     1921 aaacaggaca ggtagaagag aaagtcttga ccttggcaga tttagcccaa gcagaagcta
     1981 tttacggctg taatgcagtg agaggcttgt atgaactaat ccttgaggag aactagatga
     2041 aactgatatt tttgcatggg ttagggcagt cagcagagtc ttggaaagaa gttcggaatc
     2101 tactgacaga ttatccttct gaggctatcg agttatttcc ttctggagtt agtaactatc
     2161 aacaagctaa ggagcgagtt tatcagcacc tagctcaaga gacagaacct tttgttttga
     2221 ttggattgtc cttaggtgct gccctggcac tagaactttc cagttacgat ttaccaaatc
     2281 ttcgagcttt gattctgtca ggatgcccgc ttaaattagc agggaatatc ctattttatc
     2341 ttcagttgct gatttttaaa ctacttccca aaagagtatt tgaaaaacag ggagcagaca
     2401 aggctcttat ggtcggagtt tctgaggagt tgaagacact tgatttaacg gatatagcag
     2461 gaacttgccc ttatccaacc ttactaattt gtggtatcaa agataaaccg aatcttagtt
     2521 ctatgagaag tcttcataaa ctaatatcag aatcccaatt tcagattatc ccagatggcc
     2581 ctcatgtctt gaataaggca aaaccaaagg agtttgtaga aaagaccaga agttttcttg
     2641 aattgctgaa ataagtttga taaaataata ttgaaatcga tatcataatt atgggagaaa
     2701 gaaatgaata aacgtctatt tttaaaaatg agtctggtga cgttgccaat tttagccttg
     2761 ttttcacaac ctgtgttagc agaggaaaac attcattttt cgagttgtaa ggaagcttgg
     2821 gctaatggat attcggatct tcatgaggga gaacctggtt attctgccaa gttagaccgc
     2881 gatcatgatg gtgtggcttg cgaattgaaa aatgctccta agggtgcttt taaagcaaaa
     2941 caagcggcta caactcaaac tgatacaact tcatccacag caagtggttg ggttaagcag
     3001 gacggttctt ggtactattt tgatggaaat ggaaatctag tgaaaaatgc atggcaggga
     3061 aattattacc tgaaagctga tggtaaaatg gcacagagtg aatggattta tgactcttct
     3121 tatcaagctt ggtattattt gacatcagat ggttcttatg cttacagtac atggcaagga
     3181 aattactatc taaaatcgga tggtaaaatg gctgtcaatg aatgggttga tggtggacgt
     3241 tattatgttg gcgctgacgg agtttggaag gaaggtcaag caagtacagc ttcttctagt
     3301 aatgatagca atagtgaata ttctgctgct ttaggaaagg caaaaagtta taattcgtta
     3361 ttccacatgt caaaaaaacg tatgtataga caattaactt ctgattttga taaattttca
     3421 aatgatgcag ctcaatatgc cattgatcat ttagatgatt aacagatact ggtaggcgaa
     3481 aaaattcctt ataattcatt tacgcaaacc tttgcataag tgaatgtatt ttgatatact
     3541 agtactgtaa gcattttcaa ttactacaca atgaaaaagg agaatatgat gagtcaaaag
     3601 attattggga ttgaccttgg tggaacttct atcaaatttg caatcttaac aacagcagga
     3661 gaaatccaag gaaaatggtc aatcaagacc aacattttgg atgagggaag tcatatcgtt
     3721 gatgatatga ttgagtctat tcaacatcgt ttggacttgc ttggattggc agcagcggac
     3781 ttccaaggca ttggaatggg atcaccaggt gtggttgacc gtgacaaagg gactgttatc
     3841 ggtgcctaca acttgaactg gaaaaccctt caaccaatca aacaaaagat tgaaaaagct
     3901 ttgggcattc catttttcat cgataatgat gccaacgtag cagctcttgg tgagcgctgg
     3961 atgggtgctg gaaataacca accagacgtt gtctttatga cactcggtac tggtgttggt
     4021 ggtggtattg tcgcagaagg caaattgctt cacggtgttg ctggtgcagc aggtgagctt
     4081 ggtcatatca ctgttgattt tgaccaacca attgcatgta cttgtggtaa aaaaggctgc
     4141 cttgagacag ttgcttcagc aacagggatt gtcaacttga ctcgtcgcta tgccgatgaa
     4201 tacgaaggcg atgcagcctt gaaacgcttg attgataatg gagaagaagt aactgctaag
     4261 actgtctttg atctcgcaaa agaaggagac gaccttgctt tgattgttta ccgtaacttc
     4321 tcacgttact tgggaatcgc ttgtgctaac attggttcaa tcctaaaccc atcaacaatc
     4381 gtcatcggtg gtggtgtgtc agctgcggga gaattccttc tacaaggtgt tcaaaaagtt
     4441 tacgatgaaa atagtttccc acaagtacgc acatctacta aattagctct tgcaactcta
     4501 ggaaatgacg ctggagttat cggagcagca tcacttgtat tgcaataaga taatgagaga
     4561 ggaaaatgct aacctagagt tagtaatgtt cctcttttat tttttatgct atactagtta
     4621 ggacaatgaa tgaggtgaga gtaaatgaca aaagcagata cgatttttaa agagaatatt
     4681 gaacgcattc taaaggaagg tgttttctca gagcaggctc gtcctaagta caaggatggg
     4741 actgttgcta attccaagta tgtaactggt gccttttccg agtacgactt atctaaagga
     4801 gaatttccta tcacaacctt gcgtcccatt gcaatcaaat ccgccatcaa agaagttctc
     4861 tggatttacc aagaccagtc aaatagctta gaagtgctta atgacaagta caatgttcac
     4921 tactggaatg actgggaagt tggagacacg ggaaccattg gtgagcgcta tggtgccgtt
     4981 gttaagaaac acgacattat caataagctt ctcaaacagt tggaaaccaa tccttggaac
     5041 cgccgcaata ttatttcgct ctgggattac caagctttcg aagaaacaga tgggctgctc
     5101 ccgtgcgcct ttcagaccat gtttgatgtc cggcgtgttg atggggaaat ctatctggat
     5161 gcgaccttga cccagcgctc caatgatatg ctggtggccc accacatcaa cgctatgcag
     5221 tatgtggctt tgcagatgat gattgccaaa cattttggct ggaaggttgg gaagttcttc
     5281 tacttcatca acaacctcca tatctatgat aatcaatttg aacaagctca ggaattgctc
     5341 cgtcgggagc cgtcaaactg ccaaccacgc ttggttttaa atgttcctga tgggactaat
     5401 ttctttgata tcaaagcaga agattttgag ttggtggatt atgaccctgt taagccacag
     5461 ttgaagtttg acctagctat ttaaaagaat agaaaaaaga agttgagaat aatcccaact
     5521 tcttttgttt cttaacgtga tacgcggcga cgagctgctt ttttacggtt ttcttcgatg
     5581 aaagctgctt tttgctcttc tggttcgatt actttctttt taaatgcgta tactgcacct
     5641 gcaacggcag cgacagttcc tgcgacacct gttacaagac ctttagcgaa tcctttagcc
     5701 atgagtcttc ctcctttata ttctcaatca gccagcctcc tcaagaggtc acatttttct
     5761 gactgacctt tttgtgttat aataatagta acgaaaaaat gggaattttt caaggaaaaa
     5821 agatgaaaac aaaaataatt gtgattgttg gaccgactgc tgttggaaag acggctctag
     5881 ctattgaagt tgcaaagcgt tttaatggcg aagtggttag tggagatagc cagcaagtct
     5941 atcgaggact tgatattggg acggccaagg ctagtccaga agagcaggca gctgttcctc
     6001 atcatttaat cgatgttaga gagataaccg agtcttactc ggcttttgat tttgtttcag
     6061 aagctaagat gactattgag gatattcaca gccgtggcaa gctagccatt atcgccggtg
     6121 ggactggact ttatatccag agcttgctag aaggttacca cctaggtggg gagactcctc
     6181 atgaggagat tttagcttat cgagctagtt tggagccata ttcagatgag gaattagccc
     6241 atttggtgga gcaagcaggc cttgagattc cccagtttaa tcgtcgtcgt gctatgcgtg
     6301 ccttagaaat tgcccatttt ggtcaggatt tggaaaatca agagaccttg tatgaaccgc
     6361 tgattatctg cttggatgat gaacgtagtc aactttatga gcgtatcaac caccgagtgg
     6421 atttgatgtt tgaggctggg cttttggatg aggccaagtg gctgtttgac cattccccta
     6481 atgtacaggc tgctaaagga attggctata aggaactctt tccttatttc cgtggagagc
     6541 agaccttgga ggaagctagt gagagtctca aacaggctac tcgtcgtttt gccaagcgtc
     6601 agctgacctg gttccgtaat cgcatgcagg tcacctttta tcagattgga gaatctggtg
     6661 tacaagaccg cattttaagc cagatagagg agtttttaga tgattgaaac ggagaaaaaa
     6721 gaggagcgag tcctgctgat tggtgtggaa ttgcagggta tggacagttt tgacctctcc
     6781 atggaagaat tggctagttt agcgaaaacg gcaggggcag tcgttgtaga taactacaga
     6841 caaaaacgtg aaaaatatga ttccaagacc ttcgtcggct ctggtaagtt ggaagagatt
     6901 gcgcttatgg tggatgcaga agaaatcact actgtcatcg tcaacaaccg tctgacacca
     6961 aggcagaatg tcaatctaga ggaagttctc ggtgttaagg tcattgaccg tatgcagttg
     7021 attttggata tctttgccat gcgggctcga agccatgaag ggaagctcca agtccaccta
     7081 gcccaactca aatacctctt gcctcgcttg gttggtcagg ggattatgct cagccgtcag
     7141 gcagggggaa ttggttcccg tggtcctggt gaaagccaac tggagctgaa ccgtcgtagc
     7201 gttcgcaatc aaatcacgga tatcgagcgc cagctcaagg tggttgagaa aaatcgggcg
     7261 actgtcagag aaaaacgttt ggagtctagc acctttaaga ttggtttgat tggttatacc
     7321 aatgctggga aatcaactat catgaacatc ttgaccagta agacccagta tgaagcagat
     7381 gagctctttg cgactctgga tgcgacaacc aagagtattc atctgggagg caatctccaa
     7441 gtaactttga cagataccgt tggctttatc caagatttgc cgacagagtt ggtgtccagt
     7501 ttcaagtcaa ccttggaaga aagcaagcat gtggaccttc tggttcatgt tatcgatgct
     7561 agcaatcctt atcacgagga gcatgaaaaa acggttctct ccatcatgaa agacctggac
     7621 atggaagata ttcctcactt gacgctttat aataaagcgg atttggtgga ggatttcacg
     7681 cctacccaaa cgccatatac cctcatttct gccaagtctg aggacagtcg tgaaaacttg
     7741 caagcattat tgctagataa gattaaggaa atttttgaag catttaccct gcgagtgcct
     7801 ttttcaaagt cctacaagat tcatgattta gagagtgttg caattctgga agaacgtgat
     7861 tatcaggaag acagcgaagt gattacaggc tacatttccg aaaaaaataa atggaggtta
     7921 gaagaatttt atgactgata ttaaagcttt ggctctaaag tatggaggtt atacaagtct
     7981 ggacaaggtc tatctggatc agcttctagc tggtaaaaca gagcaggagc agttaacact
     8041 tattacacct ccgccaagtg ttgttaatgc ctactttgca gaactctatc agaaaaagag
     8101 tcctcaggct gcgacggatt attttgcaga actcagtcag gaactgaatc tttacaatac
     8161 tgagccaagt ttcaccctag aaaataagcc ttttattcgg cttaatctgt ctggtaaatc
     8221 ctttggtttt tgttatgata gtgacggcct tggtcggatt ttctctgaaa atgaggagaa
     8281 aatctcggat gacttgctct ttgaaattgc gcaaattttc ccccatcaat tagtctttga
     8341 agagtctggt aagatttaca tgaagcctgt cgaggacgag gaagttgtca gcctagaaag
     8401 tctcacagct ttgactgatt tggaaagatt ggctgatggt cgaaaacgtc tcaaaggcta
     8461 cagccaagag gatttattgc aagaagctgc tgctttttct ggcaaacgct atttccgatc
     8521 ggaaaaccgc acagccatgt tatatattga ttaattagaa agtatcgaat ggatattcaa
     8581 tttttaggaa cgggggctgg tcagccctct aaagctcgca acgtttcaag tctcgccctg
     8641 aaacttttgg atgagattaa cgaagtttgg ctctttgact gtggagaagg tacgcaaaat
     8701 cgcattctgg aaaccacaat tcgaccacgt aagatcagca aaatctttat tacccatctg
     8761 catggagacc acatttttgg tttgccaggt ttcctttcta gccgtgcctt tcaggccaat
     8821 gaagagcaga cagatttgga aatctacgga cctcaaggaa tcaagtcatt tgtcttaacc
     8881 agccttcgtg tgtcaggttc tcgtctgccc taccgcattc atttccatga gtttgaccaa
     8941 gattctctgg gtaaaattct tgaaaccgat aaattcactg tgtatgcaga ggagctggat
     9001 cacactattt tctgtgttgg ctatcgtgtc atgcaaaaag atctagaagg gacgctggat
     9061 gctgaaaaac tcaaggctgc tggtgttccg ttcggcccgc tttttggtaa aatcaaaaac
     9121 ggccaggatc ttgttttgga agacggaact gaaatcaagg cagcagacta tatctcagcg
     9181 ccacgtccag gtaagattat cactatttta ggagacactc gaaaaacgga tgccagtgtg
     9241 cgtctggctg tcaatgcaga tgtcctagtt catgagtcca cttatggcaa gggtgatgaa
     9301 aaaattgctc gtaaccatgg tcactcaact aatatgcaag ctgcacaagt agcggtagaa
     9361 gcaggtgcca aacgcctcct actcaaccat atcagtgccc gtttcctctc aaaagatatt
     9421 agcaaactca agaaagacgc tgccacaatt tttgaaaatg tccatgtggt caaagacttg
     9481 gaagaagtgg aaatctagca gtcacagaaa ggataagtat gcctactatt ctcattaccg
     9541 gagctagcgg tggtctagct caagaaatgg tcaaactcct gcccaatgac caactcatct
     9601 tgcttggtag aaataaggaa aaattagccc aactctacgg aaattattcc catgcagaat
     9661 tgattgaaat tgatattacc gacgattcag ccctagaagc tctggtaact gatctttatc
     9721 tccgctatgg caagattgat gtcttgatta acaacgctgg ttacgggatt tttgagggat
     9781 ttgaccagat tgctgataaa gatattcacc agatgtttga ggtcaatacc tttgccctga
     9841 tgaatctgtc tcgtcacctt gcggctcgta tgaaggaaag cagcaaaggg catatcatca
     9901 acatcgtcag catggcaggt ctaatagcta ctggcaagtc tagtctttac tcagcgacca
     9961 agtttgcggc tattggtttt tcaaatgctc tgcgactcga acttatgccc tatggagtct
    10021 atgtgacaac agtcaatcca ggtccaatcc gaacaggatt ttttgaccaa gctgacccag
    10081 atggaactta tcttaaatcg gttgaccgct tcctcttaga ggcagatgca gtggctaaaa
    10141 agattgtcaa gattataggc aaaaataaac gagaactcaa tctcccgatt ttgttgaacc
    10201 tagcccataa gttttatact ctctttccca agctagctga taagttggta ggggaaactt
    10261 ttaattataa gtaaaaag
//
LOCUS       DAVLYV010000071        10253 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLYV010000071 DAVLYV010000000
VERSION     DAVLYV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10253
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.071"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..679
                     /locus_tag="VYO70_001672"
     CDS             <1..679
                     /locus_tag="VYO70_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1713873.1"
                     /translation="FLKYDRAVRCRVKIITMDMFSPYYDLARQLFPCAKIVLDRFHIV
                     QHLSRAMSRVRVQIMNQFHRKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTN
                     KEILNKLLSYSEDLKHHYQLYQLLLFHFQNKEPEKFFGLIEDNLKQVHPIFQTVFKTF
                     LKDKEKIINALQLHYSNAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALNIKKERT
                     KFVLSRA"
     gene            complement(752..1603)
                     /gene="srtC-3"
                     /locus_tag="VYO70_001673"
     CDS             complement(752..1603)
                     /gene="srtC-3"
                     /locus_tag="VYO70_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-3"
                     /protein_id="HEV1713874.1"
                     /translation="MSRTKLRALLGYLLMLVACLIPIYCFGQMVLQSLGQVKGHATFV
                     KSMTTEMYQEQQNHSLAYNQRLASQNRIVDPFLAEGYEVNYQVSDDPDAVYGYLSIPS
                     LEIMEPVYLGADYHHLGMGLAHVDGTPLPLDGTGIRSVIAGHRAEPSHVFFRHLDQLK
                     VGDALYYDNGQEIVEYQMMDTEIILPSEWEKLESVSSKNIMTLITCDPIPTFNKRLLV
                     NFERVAVYQKSDPQTAAVARVAFTKEGQSVSRVATSQWLYRGLVVLAFLGILFVLWKL
                     ARLLRGK"
     gene            complement(1590..2483)
                     /gene="srtC-2"
                     /locus_tag="VYO70_001674"
     CDS             complement(1590..2483)
                     /gene="srtC-2"
                     /locus_tag="VYO70_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-2"
                     /protein_id="HEV1713875.1"
                     /translation="MSKSRYSRKKSVKKKKNPFILLLIFLVGLAVAMYPLVSRYYYRI
                     ESNEVIKEFDETVSQMDKAELEERWRLAQAFNATLKPSEILDPFTEQEKKKGVSEYAN
                     MLKVHERIGYVEIPAIDQEIPMYVGTSEEILQKGAGLLEGASLPVGGENTHTVVTAHR
                     GLPTAELFSQLDKMKKGDIFYLHVLDQVLAYQVDQIVTVEPNDFEPVLIQHGEDYATL
                     LTCTPYMINSHRLLVRGKRIPYTAPIAERNRAVRERGQFWLWLLLGAMAVILLLLYRV
                     YRNRRIVKGLEKQLEGRHVKD"
     gene            complement(2476..3372)
                     /gene="srtC-1"
                     /locus_tag="VYO70_001675"
     CDS             complement(2476..3372)
                     /gene="srtC-1"
                     /locus_tag="VYO70_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus system sortase SrtC-1"
                     /protein_id="HEV1713876.1"
                     /translation="MAKTKKQKRNNLLLGVVFFIGIAVMAYPLVSRLYYRVESNQQIA
                     DFDKEKATLDEADIDERMKLAQAFNDSLNNVVSGDPWSEEMKKKGRAEYARMLEIHER
                     MGHVEIPAIDVDLPVYAGTAEEVLQQGAGHLEGTSLPIGGNSTHAVITAHTGLPTAKM
                     FTDLTKLKVGDKFYVHNIKEVMAYQVDQVKVIEPTNFDDLLIVPGHDYVTLLTCTPYM
                     INTHRLLVRGHRIPYVAEVEEEFIAANKLSHLYRYLFYVAVGLIVILLWIIRRLRKKK
                     RQSERALKALKEATKEVKVEDE"
     gene            complement(3711..4892)
                     /gene="rrgC"
                     /locus_tag="VYO70_001676"
     CDS             complement(3711..4892)
                     /gene="rrgC"
                     /locus_tag="VYO70_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus minor pilin RrgC"
                     /protein_id="HEV1713877.1"
                     /translation="MISRIFFVMALCFSLVWGAHAVQAQEDHTLVLQLENYQEVVSQL
                     PSRDGHRLQVWKLDDSYSYDNRVQIVRDLHSWDENKLSSFKKTSFEMTFLENQIEVSH
                     IPNGLYYVRSIIQTDAVSYPAEFLFEMTDQTVEPLVIVAKKADTVTTKVKLIKVDQDH
                     NRLEGVGFKLVSVARDGSEKEVPLIGEYRYSSSGQVGRTLYTDKNGEIVVTNLPLGTY
                     RFKEVEPLAGYTVTTMDTDVQLVDHQLVTITVVNQKLPRGNVDFMKVDGRTNTSLQGA
                     MFKVMKEENGHYTPVLQNGKEVVVASGKDGRFRVEGLEYGTYYLWELQAPTGYVQLTS
                     PVSFTIGKDTRKELVTVVKNNKRPRIDVPDTGEETLYILMLVAILLFGSGYYLTKKTN
                     N"
     gene            complement(4957..6954)
                     /gene="rrgB"
                     /locus_tag="VYO70_001677"
     CDS             complement(4957..6954)
                     /gene="rrgB"
                     /locus_tag="VYO70_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus major pilin RrgB"
                     /protein_id="HEV1713878.1"
                     /translation="MKSINKFLTMLAALLLTASSLFSAATVFAAGTTTTSVTVHKLLA
                     TDGDMDKIANELETGNYAGNKVGVLPANAKEIAGVMFVWTNTNNEIIDENGQTLGVNI
                     DPQTFKLSGAMPATAMKKLTEAEGAKFNTANLPAAKYKIYEIHSLSTYVGEDGATLTG
                     SKAVPIEIELPLNDVVDAHVYPKNTEAKPKIDKDFKGKANPDTPRVDKDTPVNHQVGD
                     VVEYEIVTKIPALANYATANWSDRMTEGLAFNKGTVKVTVDDVALEAGDYALTEVATG
                     FDLKLTDAGLAKVNDQNAEKTVKITYSATLNDKAIVEVPESNDVTFNYGNNPDHGNTP
                     KPNKPNENGDLTLTKTWVDATGAPIPAGAEATFDLVNAQTGKVVQTVTLTTDKNTVTV
                     NGLDKNTEYKFVERSIKGYSADYQEITTAGEIAVKNWKDENPKPLDPTEPKVVTYGKK
                     FVKVNDKDNRLAGAEFVIANADNAGQYLARKADKVSQEEKQLVVTTKDALDRAVAAYN
                     ALTAQQQTQQEKEKVDKAQAAYNAAVIAANNAFEWVADKDNENVVKLVSDAQGRFEIT
                     GLLAGTYYLEETKQPAGYALLTSRQKFEVTATSYSATGQGIEYTAGSGKDDATKVVNK
                     KITIPQTGGIGTIIFAVAGAVIMGIAVYAYVKNNKDEDQLA"
     gene            complement(7177..9837)
                     /gene="rrgA"
                     /locus_tag="VYO70_001678"
     CDS             complement(7177..9837)
                     /gene="rrgA"
                     /locus_tag="VYO70_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-1 pilus tip adhesin RrgA"
                     /protein_id="HEV1713879.1"
                     /translation="MKKVRKIFQKAVAGLCCISQLTAFSSIVALAETPETSPAIGKVV
                     IKETGEGGALLGDAVFELKNNTNGTTVSQRTEAQTGEAIFSNIKPGTYTLTEAQPPVG
                     YKPSTKQRTVEVEKNGRTTVQGEQVENREEALSDQYPQTGTYPDVQTPYQIIKVDGSE
                     KNGQHKALNPNPYERVIPEGTLSKRIYQVNNLDDNQYGIELTVSGKTVYERKDKSVPL
                     DVVILLDNSNSMSNIRNKNARRAERAGEATRSLIDKITSDPENRVALVTYASTIFDGT
                     EFTVEKGVADKNGKRLNDSLFWNYDQTSFTTNTKDYSYLKLTNDKNDIVELKNKVPTE
                     AEDHDGNRLMYQFGATFTQKALMKADEILTQQARQNSQKVIFHITDGVPTMSYPINFN
                     HATFAPSYQNQLNVFFSKSPNKDGILLSDFITQATSGEHTIVRGDGQSYQMFTDKTVY
                     EKGAPAAFPVKPEKYSEMKAVGYAVIGDPINGGYIWLNWRESILAYPFNSNTAKITNH
                     GDPTRWYYNGNIAPDGYDVFTVGIGINGDPGTDEATATSFMQSISSKPENYTNVTDTT
                     KILEQLNRYFHTIVTEKKSIENGTITDPMGELIDLQLGTDGRFDPADYTLTANDGSRL
                     ENGQAVGGPQNDGGLLKNAKVFYDTTEKRIRVTGLYLGTGEKVTLTYNVRLNDQFVSN
                     KFYDTNGRTTLHPKEVEKNTVRDFPIPKIRDVRKYPAITIAKEKKLGEIEFIKINKND
                     KKPLRDAVFSLQKQHPDYPDIYGAIDQNGTYQNVRTGEDGKLTFKNLSDGKYRLFENS
                     EPAGYKPVQNKPIVAFQIVNGEVRDVTSIVPQDIPAGYEFTNDKHYITNEPIPPKREY
                     PRTGGIGMLLFYLIGCMMMGGVLLYTRKHP"
BASE COUNT         2892 a         2377 c         1661 g         3323 t
ORIGIN      
        1 ctttcttaaa tatgatagag ccgtccgatg tcgcgtcaaa atcattacta tggatatgtt
       61 tagtccttac tatgacttag ctagacaact tttcccgtgt gctaaaatcg ttcttgatcg
      121 ctttcacatt gtacaacatc ttagccgtgc tatgagtcgt gtgcgtgttc aaatcatgaa
      181 tcagtttcat cgaaaatccc atgaatacaa ggctatcaag cgctactgga aactcattca
      241 acaggatagc cgtaaactga gtgataagcg attttatcgt cctacttttc gtatgcactt
      301 aaccaataaa gagattttaa acaagctttt gagctattcg gaagacttga aacaccacta
      361 tcaactctat cagctcttgc tttttcactt ccagaataag gaaccagaga aatttttcgg
      421 acttattgag gacaatctta agcaggttca tcctattttt cagactgtct ttaaaacctt
      481 cctcaaagat aaagaaaaga ttatcaacgc ccttcaacta cactattcta atgccaaact
      541 ggaagcgacc aataatctca tcaaacttat caagcgcaat gcctttggtt ttcgaaactt
      601 tgaaaacttc aaaaaacgga tttttatcgc tctgaatatc aaaaaagaaa ggacaaaatt
      661 tgtcctttct cgagcttagc tttttttcaa cccactacag ttgacaaaga gccggaaaaa
      721 ggaacagcct tagctttcct ttcatttctt tttatttccc tcgtagtaaa cgtgctagct
      781 tccacaaaac aaacaggatt cccagaaatg ccagtaccac tagcccacgg tacaaccatt
      841 gagaggttgc aacacgcgat acagattgtc cttctttcgt aaaagcaacc ctcgcaactg
      901 cagctgtttg tggatctgat ttttgataaa cagcgactcg ttcaaaattc actaataagc
      961 gtttattaaa ggtaggaatc ggatcgcagg ttatcaaggt catgatattt ttagagctaa
     1021 ccgattctaa tttttcccat tccgacggta aaataatctc tgtgtccatc atctgatatt
     1081 ctacaatttc ctggccatta tcataataaa gagcatctcc aacttttagc tgatccaaat
     1141 ggcggaaaaa gacatggctt ggctctgcac ggtgcccagc aatcactgag cgaatccctg
     1201 taccatccag aggcagcggt gtaccatcca catgagccaa gcccatccct aaatgatgat
     1261 aatctgctcc caaataaacc ggctccatga tttccaaact tggaatagac aagtaaccat
     1321 agactgcatc agggtcgtca gacacttggt aattgacctc atatccctcc gccaaaaaag
     1381 gatctacaat gcgattttgc gaagccaagc gttgattgta ggcgagagaa tggttctgtt
     1441 gttcttggta catttcagtt gtcatggatt tcacaaatgt agcatgacct ttcacctgtc
     1501 caagagactg caacaccatc tgtccaaaac aataaatagg aatcaaacag gctaccaaca
     1561 tcaacaagta tcccaataag gctcgtagtt tagtccttga catgacgccc ctccaattgc
     1621 ttttctagtc ctttgacaat ccgtcgatta cgatacacgc gatacagcaa gagaaggatg
     1681 accgccatcg ctcctagtaa taaccacaac cagaattgcc cacgctctct caccgctcga
     1741 ttccgctctg caattggtgc cgtatacgga atccgcttcc cacgtaccaa cagacgatga
     1801 ctgttaatca tatacggtgt acaagtcaac aaggtcgcat aatcttcccc atgttgaatc
     1861 aagacaggct caaagtcatt cggctccacc gtcactatct gatccacttg gtaggccaac
     1921 acctgatcta aaacgtgaag ataaaagata tccccttttt tcatcttatc caattgacta
     1981 aacagttctg ccgtcggtaa tcctctatga gcagtgacaa ctgtgtgggt attttcacca
     2041 ccaaccggta acgaagctcc ctctagcaat cctgcgccct tctgaagaat ttcctcactc
     2101 gttccgacat acatcggaat ttcctgatca atcgcaggaa tttccacata gccaatccgc
     2161 tcatggacct ttagcatatt ggcatattct gagacgcctt tctttttctc ttgctctgta
     2221 aaaggatcaa gaatttcaga tggtttcaag gtcgcattga aggcttgagc caagcgccaa
     2281 cgctcctcaa gttccgcctt atccatctgg gaaaccgtct catcaaactc tttaataacc
     2341 tcgtttgact caatacgata ataataacga gacaccaagg gatacatcgc aacggcaagc
     2401 cccaccaaaa aaatcagaag aagaatgaac ggatttttct tctttttcac gctctttttc
     2461 cgtgaatatc tactcttact catcctctac cttcacttcc ttagtagctt ccttcaatgc
     2521 tttcaaagct ctttctgatt gccgtttctt cttgcgcaag cgtcgaataa tccataaaag
     2581 aatcacaatc aaaccaactg ccacataaaa caggtagcga tagagatgac tgagtttgtt
     2641 tgccgcaata aattcttcct caacctctgc tacgtacggt atccgatgcc cccgaaccaa
     2701 tagacgatgg gtattgatca tgtatggcgt acaggtcagc aaggtcacat aatcatgacc
     2761 tggtacaatc aataaatcat caaagttcgt cggctcaatc acctttactt gatccacttg
     2821 ataggccatc acttccttga tattgtgcac ataaaactta tccccaactt taagtttggt
     2881 caaatccgta aacatcttag ccgttggcaa acctgtatgt gccgtaatca ccgcatgggt
     2941 cgaattgcct ccgatcggta gagaagttcc ctctagatgt ccagcgcctt gctgcaatac
     3001 ctcttcagca gtaccagcat aaaccggcaa atctacatca atagcaggga tttccacatg
     3061 ccccatccgc tcatggattt ctaacatgcg tgcatactct gctcgccctt ttttcttcat
     3121 ttcttccgac caaggatcgc cactcactac attattcaaa gagtcattga aggcttgtgc
     3181 caatttcatt cgttcatcaa tgtcagcctc atccaacgtt gctttttcct tatcaaagtc
     3241 agcaatttgt tgatttgatt ccactcgata atacaagcga gacaccagcg gatacgccat
     3301 taccgctatt ccaatgaaaa ataccactcc taataggaga ttgtttcgtt tttgcttttt
     3361 tgtttttgcc attttttatc agcattcctt taacttcaaa ctcatcattt tatcgctttt
     3421 cctaacagac aatcctatct gctaagagaa acgagaaaat ctcatttctt tctgattcac
     3481 catccatttt aaatcagaat cccagtcctg tccattccta aaatgattac cagaccagtc
     3541 ctatcgtttt acaaacaata acccacaatg aggctggcac aatctgccca gcctcatatc
     3601 cgttattatg attcatgcca aatcaccatg attcctgatt aacatcacct cagagtactc
     3661 tggtcttccc ttcagcctgc tatctttttc ataatgatgt acattgaata tcagttattt
     3721 gtttttttcg taagataata gccactacca aacaacaaaa tggcaacaag catcaagata
     3781 tacaaggttt cttcccctgt atctggcaca tcaatccgtg gtcgcttgtt atttttaacc
     3841 actgttacca gttccttacg agtatctttc ccgattgtaa aggaaacagg cgatgttaat
     3901 tgaacataac cagttggagc ttggagctcc cataaatagt atgtcccata ctctagacct
     3961 tccactcgga aacgaccatc tttccctgat gccacaacta cttccttacc attttgaaga
     4021 acaggagtat agtgtccgtt ttcttctttc atgactttga acattgcccc ttgaagagag
     4081 gtattggtcc taccatccac cttcataaag tcaacattgc cacgtggtaa tttctgattg
     4141 acaaccgtaa tcgtcaccag ctgatgatct accaactgga catccgtatc catcgtcgta
     4201 acagtatagc ctgccagtgg ctccacctcc ttgaaacgat aggtcccaag aggaagattt
     4261 gtcacaacaa tctctccatt tttatcagta tagagagttc tccctacttg accagaagaa
     4321 ctgtaacggt attctccaat caagggaacc tctttttcag aaccatctct tgctactgat
     4381 accaatttaa agccgacacc ctccaagcga ttgtggtctt gatccacctt tatcagcttc
     4441 acctttgttg tcaccgtatc tgcttttttc gctacaatga ccaaaggctc taccgtctga
     4501 tctgtcattt caaaaagaaa ttcagctgga taagaaaccg catccgtctg gataatagag
     4561 cgaacatagt aaagaccatt tggaatatga gatacttcaa tctgattctc aaggaaggtc
     4621 atctcaaacg aagtcttttt gaaagaagaa agtttattct catcccacga atgcaagtct
     4681 ctcacaattt gcacccgatt atcataggaa tacgaatcat ccaacttcca tacctgcaac
     4741 cgatgaccat cacgagatgg caattgacta accacctcct gatagttctc caattgcaag
     4801 accaacgtgt gatcttcttg cgcttggact gcatgtgcac cccatacaag agaaaaacac
     4861 agagccataa caaagaagat acgactaatc attttctgca ttttctgcat tttctgcatt
     4921 ttctgcattg tcatcaatgg ctcctttctc tcttacttaa gcaagttgat cctcatcttt
     4981 gttgttttta acatatgcgt acactgcaat acccataatc acagcccctg ctacagcaaa
     5041 gataattgta ccaataccac ccgtttgtgg gatcgtgatt tttttgttga ctacttttgt
     5101 agcgtcatct ttacctgaac cagcagtata ctcaatgcct tgtccagtcg ctgaataaga
     5161 agttgcagtg acttcaaatt tctgacgact agttagtaat gcataaccag caggctgttt
     5221 tgtttcttct aagtaatatg tacctgcaag aaggcctgta atttcaaagc gaccttgtgc
     5281 atcagaaact aatttcacaa cattttcatt gtccttatct gccacccatt caaatgcatt
     5341 gttggcagca atcacagcag cattataagc agcttgagct ttgtcaactt tctctttttc
     5401 ttgctgagtt tgttgttgtg cagtaagagc gttataagca gcaactgctc tatctaaagc
     5461 atcctttgtt gtaacaacca actgcttctc ttcttgactc actttatctg ctttacgtgc
     5521 taaatattga ccagcattat cagcatttgc aattacaaat tctgccccag ctaaacgatt
     5581 atctttatca ttaactttga caaacttttt accatatgta acaacttttg gctctgttgg
     5641 atcaagtggt tttggatttt cgtctttcca gttcttgaca gcaatttctc cagctgtagt
     5701 gatttcttga taatctgctg aatacccttt tatactacgt tcaacgaatt tatattctgt
     5761 atttttatcc aatccgttaa cagtaactgt atttttgtct gttgtcaaag ttacagtttg
     5821 tacaacttta ccagtctgag cattaaccaa atcgaacgtt gcttcagctc cagccggaat
     5881 tggtgcacct gtagcatcaa cccatgtctt ggtcaatgtc aaatcgccgt tttcatttgg
     5941 cttattcggc tttggagtat tcccgtgatc tggattatta ccatagttaa atgttacatc
     6001 attagattct ggtacttcta caattgcttt gtcattcaat gttgccgaat aagtgatttt
     6061 cacagttttt tcagcgtttt ggtcattcac tttagctaaa ccagcatctg ttaatttcaa
     6121 atcaaaacca gttgctactt ctgttagagc ataatcacct gcttcaagtg caacatcatc
     6181 aacagttact ttcactgtac ctttgttgaa tgccaaacct tcagtcattc tatcgctcca
     6241 gtttgctgtt gcataattag caagtgctgg aatttttgta acaatttcgt actctacaac
     6301 atctccaact tggtggttca caggtgtatc tttatctaca cgtggtgtat ctggatttgc
     6361 tttacctttg aaatctttat caatttttgg ctttgcttct gtattttttg gatacacatg
     6421 cgcatccaca acatcgttca atggtaattc aatttcaatt ggaactgctt tagaacctgt
     6481 taaggttgct ccatcttcac cgacataagt tgataaactg tgaatttcat aaattttata
     6541 cttagcagct ggtaaatttg ccgtgttaaa tttagctcct tcagcttctg ttaatttttt
     6601 cattgcagtt gccggcattg cccctgagag tttaaatgtt tgtggatcaa tattcactcc
     6661 tagagtttgg ccattttcat caataatttc attattagta tttgtccaaa cgaacataac
     6721 accggcaatt tcttttgcat ttgcaggtag aacacccact ttattaccag catagttacc
     6781 tgtttctaac tcatttgcaa ttttatccat atccccatct gttgccaata gtttatgaac
     6841 ggtaacagat gttgttgtcg tcccagccgc aaaaactgtt gcagctgaaa acaggctact
     6901 cgctgtcagt aataaggcag caagcattgt taaaaatttg ttgattgatt tcatgatttc
     6961 tccttattta tctcttaaaa tcaactgtga gcaatgtaat gaattacaca tgacaataat
     7021 aatccttttt ctgtgcaagt tttcgaaaac atctcccctt tcaatggtgg tttacttggc
     7081 gaagaaaact ggtttcacca agtaaactta aatctcttct tgagtgctac ttcttaaaag
     7141 tatcgttcag aacatagata ttatcatttc tacactttac ggatgtttcc gtgtgtataa
     7201 tagaactcct cccatcatca tgcaacctat cagatagaat agcaacattc cgataccacc
     7261 agttcgagga tattctctct ttggaggaat aggttcattg gtaatatagt gcttatcatt
     7321 cgtaaactcg taacccgctg gtatatcttg tggaacgatt gaagtcacat ctctgacttc
     7381 tccatttact atttggaagg caacgatagg cttattttga acgggtttat aaccagctgg
     7441 ttcagaattt tcaaataatc gatatttccc atctgacaga tttttaaagg tcaacttacc
     7501 atcttcacct gttctcacat tttgataagt gccattttga tcaatagctc cataaatatc
     7561 tggataatcc ggatgttgtt tttgaagact aaagaccgca tctctcagtg gttttttatc
     7621 attcttattg atcttaataa actcaatttc accaagtttt ttctcttttg caatcgtaat
     7681 tgctggatat tttcgcacat cacgaatctt aggaatcggg aagtcgcgca ctgtgttctt
     7741 ttctacttcc ttagggtgta gggttgttcg accattcgtg tcatagaatt tattgcttac
     7801 aaattggtca ttcaagcgaa cattataagt caatgtaact ttttcacccg ttccaaggta
     7861 caaacctgtt acacgaatcc ttttctcagt cgtatcatag aacacttttg cattttttag
     7921 caagccacca tcattttgtg gaccacctac agcttgtcca ttctccaagc gactaccatc
     7981 gtttgcagtt aaagtgtaat ctgctggatc aaatcttcca tctgtgccca attgcaaatc
     8041 aattaactca cccatcggat ctgtaatcgt accattctca attgatttct tttcagttac
     8101 gatggtgtgg aaataacgat tcaactgttc caatattttt gtcgtgtcag taacattggt
     8161 atagttttca ggtttactag aaatactttg cataaaacta gtagccgttg cttcatccgt
     8221 accaggatct ccgttaatac caatacctac cgtaaagaca tcatacccat caggagcaat
     8281 attcccgtta tagtaccatc ttgtagggtc accatgattg gtaattttag cagtattaga
     8341 attaaacgga taagccagaa tactctctct ccaattaagc caaatatatc caccattaat
     8401 tggatcgcct ataactgcat aaccaaccgc cttcatttca gaatattttt caggtttaac
     8461 tgggaaagct gcaggagcac ctttttcata aactgtctta tctgtaaaca tctggtaact
     8521 ttgcccatct ccgcgtacaa ttgtatgttc tccactagtt gcttgcgtaa taaaatcact
     8581 taatagtatt ccatctttat taggagattt actaaaaaat acatttagtt gattttgata
     8641 tgatggagca aacgtagcat gattaaaatt aatcggatac gacatagttg ggacaccatc
     8701 cgtaatatgg aaaatgactt tttgactatt ttgtctcgct tgttgtgtca aaatctcatc
     8761 tgccttcatc aaagctttct gagtaaaagt ggcaccgaat tggtacatca atctatttcc
     8821 atcatggtct tctgcctcgg taggtacctt attttttaat tctacaatgt cattcttatc
     8881 attagtcagc tttaaataac tataatcttt ggtattggtt gtaaaactcg tctgatcata
     8941 attccaaaaa agagaatcat tcaatcgttt tccgttttta tctgctaccc ctttttctac
     9001 tgtaaactcg gtcccatcaa agatagtgga agcataagtc acaagcgcta ccctattttc
     9061 tggatcagat gtaattttat caataagaga acgtgtcgcc tcaccagctc tttccgcacg
     9121 tcgagcattc ttgtttcgaa tgttactcat actatttgag ttatcgagca agataacgac
     9181 atccagcggc acagacttat cttttcgttc atacactgtt ttcccactaa ccgtcaattc
     9241 gattccatat tggttatcat ccaaattatt cacttgataa attctctttg aaagtgtacc
     9301 ttctggaatc acacgttcat atggattcgg attcaacgcc ttgtgctgtc cgtttttttc
     9361 cgaaccatct accttaataa tctgataagg tgtttgaaca tctggataag tccctgtttg
     9421 tggatactgg tcagatagag cctcttctcg attttctacc tgttcacctt ggacagtcgt
     9481 ccgaccattc ttctcaactt caacagtccg ttgtttagta gagggtttat aaccaactgg
     9541 aggttgggct tctgtcaagg tgtatgtccc aggttttatg tttgaaaata tcgcttctcc
     9601 tgtttgcgcc tctgtccttt gcgaaacagt tgtgccattc gtattgtttt tcaactcaaa
     9661 gacggcatct cctagaagcg ctcctccttc gcctgtctcc ttaatcacta cttttcctat
     9721 cgctggactg gtttcaggcg tttctgctaa agcaactatc gaagaaaaag ctgtcaactg
     9781 agatatacag cacagtcctg caactgcctt ctgaaatatc tttcttactt ttttcatgtg
     9841 tgtctccctg ttaagcatta ttcatagaac aagttaatcc ttctatgatt cataggtact
     9901 ttcaatctgt atacacgaag aaacggatta cttatgttct gataaaattt atctcactga
     9961 gttaatccgt tcactaacaa acgtagcaaa agcgtataaa agtgcgttat agctatcttc
    10021 tcaacctggc aatgacacac ttgtatacaa tatagtatag tatagtatag tattactatt
    10081 aactatccta gtataaatta aaacaacttc catcacaata gatattttta tcacatattt
    10141 tttatagaac gacaatattt gataaaaaat attttctaat aaaaaaaata agccataaaa
    10201 ctccaccaat actcattact atctttaaaa tgccttttat tacatgctgt ttt
//
LOCUS       DAVLYV010000072        10161 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLYV010000072 DAVLYV010000000
VERSION     DAVLYV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10161
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.072"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..210)
                     /locus_tag="VYO70_001679"
     CDS             complement(<1..210)
                     /locus_tag="VYO70_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713880.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILF"
     gene            complement(210..974)
                     /locus_tag="VYO70_001680"
     CDS             complement(210..974)
                     /locus_tag="VYO70_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713881.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVATLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVTYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(1070..1473)
                     /locus_tag="VYO70_001681"
                     /pseudo
     CDS             complement(1070..1473)
                     /locus_tag="VYO70_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1653..2285)
                     /locus_tag="VYO70_001682"
     CDS             complement(1653..2285)
                     /locus_tag="VYO70_001682"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1713882.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVDAIKEAFPEVEVIAGTATAGIPHGAIIADKMNLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(2319..3020)
                     /gene="pyrF"
                     /locus_tag="VYO70_001683"
     CDS             complement(2319..3020)
                     /gene="pyrF"
                     /locus_tag="VYO70_001683"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1713883.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPIIQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            complement(3286..4452)
                     /gene="divIB"
                     /locus_tag="VYO70_001684"
     CDS             complement(3286..4452)
                     /gene="divIB"
                     /locus_tag="VYO70_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1713884.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESN"
     gene            complement(4462..5520)
                     /locus_tag="VYO70_001685"
     CDS             complement(4462..5520)
                     /locus_tag="VYO70_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1713885.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGNKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(5524..6876)
                     /locus_tag="VYO70_001686"
     CDS             complement(5524..6876)
                     /locus_tag="VYO70_001686"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1713886.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQCLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVAFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(6980..7621)
                     /locus_tag="VYO70_001687"
     CDS             complement(6980..7621)
                     /locus_tag="VYO70_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713887.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLSLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(7623..9644)
                     /locus_tag="VYO70_001688"
     CDS             complement(7623..9644)
                     /locus_tag="VYO70_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1713888.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(9715..10014)
                     /locus_tag="VYO70_001689"
     CDS             complement(9715..10014)
                     /locus_tag="VYO70_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1713889.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
BASE COUNT         3072 a         2222 c         1802 g         3065 t
ORIGIN      
        1 gaacaatata tatgtgaacg gaaagcaata tactccagtc gtcatatctt gaagtaaaac
       61 taaaatccat tctaatacat ttggatggat tgaatattgg cgacagcgca ataaatatac
      121 tgtactagat aaaacacagg atagcagtaa tataaaataa accaatactg ctaagaaatc
      181 ttttttataa aattgaacaa attgtttcat tatacatagt cctctgaatg tagaaaaaat
      241 gtaccataaa caaccaaaca actaacaaat aaaataaaag caagatgccc actaactaag
      301 gaaagactga tatctttctg atatcccaaa gctaatgttg tcacaggttc taagtaagat
      361 agccctaaaa tagcccaaaa aataccacca accatcatat aggtaactgg gatgaaaata
      421 gctcctattt ttttcttcac tagcaaagca ctagctagtc caaaaataga gaacacagcg
      481 ccccaaactc cataccaaag agtcgccaca agactataga gcaactgatt agaatcaaat
      541 aattctttta aggcaccact ataatctcca atataaattt cctgataagg agtcactaaa
      601 agattaattc ctaataataa taaatagggg agaaaaaaga ctagaaaaga agaaataatt
      661 gcagtactac ctacaatagc tagatacctc tttttagaaa ttcggcacaa ttgtgctgtt
      721 agaaaatgac tctcagcatc ctctattatc tgactagaat agggcagtgt acagataagt
      781 gcagctacta ggctaatcgg tgaaaatcct cgaatagaag aagcggcaaa aaaaatcgat
      841 aaaccttcaa ttttataaat accattgaaa gcaaggaaat ttcctaaact catgcaaaga
      901 agggtcaaaa ataaagacat ataaaatcga ggtgattgaa cgactcgtac aagattacaa
      961 atgaaaaatt ccatccttac tcctccttat aataaaaata gggtgtagca ttcttttttc
     1021 atgctacacc cacaatcaac catctttaag gcttactctg acaagtaagt taataagaat
     1081 ctggactcca agaacctgaa gtatgaattc ttacatgatt tccaaattgt ggcgccatag
     1141 ctaatctagt accagaacca atataattgt caccacctcc attatagtac atgacaatcc
     1201 tagagccaga ccccaatgaa tataccgggg taatatctga cccactatag gcgctacgaa
     1261 tagaggtact taacctttta ccgccaccag tgctgtcact attattaatt ccagcagagg
     1321 cgttttcttt ctcaagctca ctagttgtat ccgaacccca acgcggcaac gttaaatcat
     1381 aatttccaat ctgtctagct aaaactgtag ggagaaacaa actagatgca attgtaacta
     1441 cgcaaaaaag gaatatattt ttcttctttt catgacatcg tcctcttaag aagtgataaa
     1501 caagatagtt gattgatcaa tttgatacat attacatttt cattatatca ataaaagaaa
     1561 gcgttgtcaa ttattttatt taaaatattg tcatatttta aataaaatgg taaaagaggc
     1621 tcggtagcct cgtctctctt tatcttactg acctaacctt cttgccaatt ttcttggtct
     1681 tctttaaagc gttttagaag atcaaggccc tctggcgtga tgtaaccttc ttcttgggct
     1741 agatggataa gctcgctata gtttgaaagc gtcacaagtt taacaccagc atctgcaaag
     1801 ttcttatctg cttttggcaa ttggtagctg aaaatcgcta caactccaag tacatctgct
     1861 ccttctcgct tggctgctgc tacagcttca agaactgaac cacccgttga aataaggtct
     1921 tcaaccacta ccattttttg accttgagct acgcgacctt cgatttgatt accagctccg
     1981 tggtcttttg gtttgctacg gatgtaggca aaaggcaagt tcatcttatc agcaataatg
     2041 gctccgtgtg gaatccctgc tgttgcagtt cctgcaatca cttctacttc aggaaaggct
     2101 tctttgatag catccacaaa accattttca attagggtac gagtttctgg ataggctagt
     2161 gtcacacgat tatcagtgta aatcggtgac ttgataccag atgcccaagt gaagggttcc
     2221 tctggtttga ggtaaacggc ttggattttc aagaggtggc tagcgatatc tttagcaagt
     2281 gtcatggtat tctcctttta tttttctaat ctagttcttt aattccagtc ctgtgtccat
     2341 tcatccttga tggtatgata agctgcaaca ggatcctcag cttggataat gggacgtccc
     2401 actacgatat agtcactgcc gatttgatag gcatcagcag gtgtcatgac tcgtttttga
     2461 tctccaactg caacaccagc tggacgaatc cctggtgtca gacagataaa atctggattg
     2521 gtagcctgct tgatgacttg tacttcctga gccgagcaaa caacaccatc caagccagct
     2581 tcagctgtct tcttggcata gtgaatcaca gactcttgca gactggtttg gatattttga
     2641 aactcctgca tctgagcttc tgacgttgat gtgagctgag ttacagcgat caatttagct
     2701 tgacttccaa gaccttcacg cgcagccttc atcatctcta caccaccagc cgcatgaaca
     2761 ttggtcatat cgacaccaag ctgagatagg atcttcattg ctgacttgac tgtattagga
     2821 atgtcatgaa gtttgagatc caaaaagaca ctatgaccca aaccttttaa gtaggacaca
     2881 atctcaggcc ccgctgcgta ataaagctcc atccctacct tgagataaag gctttcttct
     2941 gctgggaaaa gagctaaaaa ttccttgacc gcctcaaaac taggaaaatc aagagcaatg
     3001 attggacgat gttctcgcat aggtttctcc ttttaccttt actagtgaga gagtctggcc
     3061 aacagaagcc acgaaaaaag gaacctgcca acagcaaggt tccacgaaaa atgggtagtc
     3121 tccacccttt caccgtctaa ccttagctgc ctctctggac tgctttaaag gttttctatt
     3181 attctattat ttctactagc acttgtcaag taaaaacatg gtcactaaag aactataaga
     3241 gaaaaggtaa acctagcgac gcgatgagcg ctgggtcgtt tgatttcaat tgctctcttc
     3301 ctcttgtttt ttctgttctt cttcctgttt tttctcggct tccttggcct cttgtttggc
     3361 tttttcctca gcctccataa ttaatttatc cgccacagtg taactgtaaa ttccagcttc
     3421 catgtcgacc acactcggtt ctgacaattg tggcttaatc ttactgtaat atggcagttt
     3481 cttactcatt tcagatagag gaaccaagac ttcgtccgaa tcattcatgg tcaatcgaat
     3541 taaatcggat gtcaccttgc ttggggctaa ttccaccttt tggatagccg ccttgagttc
     3601 tgggctaatt tgagcaagtt ctgagacaaa aaccttgatt tgttcactat cattaaagag
     3661 aactgataaa taagtttctg gtaaactgtt cagactcaca gaactagtct caagctgacc
     3721 actggaaaga ataggataat gattttcacc agaaatatag taggccacaa tatcatattc
     3781 cttgacctta atagtgaact tagttggaaa ttgatagaca agttgagctg attcaaccca
     3841 atagttagac ttaatctgct tttcatattt tgccttgtct agcagaagat taatcgtata
     3901 atccgaatcc tgaatgcctg aagcctgtcg aatatcatca gctgtagttt gcactgttcc
     3961 ctcaacacga atatctttca tggtcgcata aggactgagc aagtaggcag agacaatcaa
     4021 taaaagcaga cttggaaata aaatcgtgaa ggctcgcaag atatggatac caggaatctt
     4081 tgctttggct ggtttttcct ttgtagcctt tttagaaagc tttttatcct gttcctcctt
     4141 ctctttagac tctggttctt ctttctcttc tttctctttg tcagcctctg aggatgctac
     4201 tttttcttca gactcttcct tagctgattc tgaatcttcc tggtctgttt cactctcctg
     4261 gtcctgttta tcctctgact tctcagattc ttctcccatt cgagcttgtc tttccttttc
     4321 cttctcctca gctagagccg cctcttcttc agccttcttt tttagatatt cttggtttcg
     4381 tttctgccat tctgataact ctttcaattc ttcgagggtt tctttgtcct catttttctt
     4441 atcttttgac atttactttc cttatgataa atcttttttc aacaattgat aaaaatctgc
     4501 tagagatttc aattccttag aagctttcat cttagcttgg taatcttcct tgtgacttag
     4561 taagtgagaa agcttctctt ccaaactatc caaggtcaaa tcgctttctt gaaggtcttc
     4621 tgcatagcct ttttttacaa agtaagctgc attttcaatc tggtcaccac gactagcttc
     4681 acgaccaagc ggaacaatga catgcaattt tgctatcgcc aagagctcaa aaatcgtatt
     4741 ggcaccacct cgtgtcacaa caatatcagc caattccatc aagggttgat agagatcggt
     4801 cacatagtca acacgaaaaa gattttgcct caactcattc agactagaat ctccagttag
     4861 attgataata ttgtagcgct ctgttagttc tttcttatgg tctgtcacca attggttaaa
     4921 gacacgagcg cctgcagaac cgccgacaaa caatacagtt ggcaatttgg gattaaagtg
     4981 ggtttgaata tccaccaatt catctggttc tggagtgttt ttgtccgaaa ccttggtcac
     5041 cgctcccaca tgctcgacct tagccaaact cgaagcttgt tcaaaggttg aatacatctt
     5101 agtcgcaaat ttataggcga ttttattggc caagcccata gacaggtcag attcgtgaat
     5161 aaagacaggc actcctgaca cacgcgcagc gataacaggc ggtactgaga caaagcctcc
     5221 ctttgaaaaa agggtctgtg gacgaagtcg caacatgata aagagcgatt ggacgattcc
     5281 ccagccaact ttgaagacgt ccagcatatt ttgccaagag aaatagcgac gcaatttccc
     5341 agtcgcaatg gagtggaaag tgacatccaa acctgactta aggatttctt ggtgttcgat
     5401 accacgcttg ttcccgatat agtggacttc ccaaccatct tcgatgaact tgggcattaa
     5461 caaaagattg agggtaacgt gtccaaccgt ccccccacct gtaaagacaa tttttttcat
     5521 attttattct tttaactccg ctactgtgtc gataaagagg tcgccacgta cttcaaagtt
     5581 agcatacata tcccagctgg cattggcagg actaagaaga accacatctc cttgagtcgc
     5641 aagctcatag gccttgcggg tcgcatctgc aatatctgtc gcctccacat aagcgacacc
     5701 agccttgtct gctgcccgtt tgacacgttc tgcagattga cccaggatga ccatcttctt
     5761 gagtccagta atgtctggca ccaattcgtc aaactcattg ccacggtcca aaccacctgc
     5821 aatcaagacg accttgctgt tgtcaaatcc tgacaaggct ttttgagtag ccaagatatt
     5881 agttgattta ctgtcgttat agaatttaac acccttgatg tcatccacaa actggagacg
     5941 gtgtttgaca ccaccgaagg ctgaaagagt ttccttgatg gtttgattgt ccacatcacg
     6001 aagcttggct acagcaatag tcgcaagggc attttccaca ttgtggctac ctggaacacc
     6061 gatttcattc gctgccatga ctacttcacc acggaagtag agttgaccat cttccagata
     6121 agctccatca accttttcaa gtgttgaaaa tgctacaaca gtggcttctg tcttggaagt
     6181 caagtctttt gccaagtctt gattaaagtt caagacaagg aaatcagctg ctgtcatctt
     6241 gttctggata ttccacttgg ctgctacata ttccgaaaat gacccatggt agtcgatatg
     6301 agttggcatg aggttggtaa taaccgcaat ctctggatga aattcttgaa cacccatgag
     6361 ttggaaagaa gaaagttcca taacaagcgt gtccttgtcc gacgcagttt gggccacttg
     6421 actagctgga tagccgatat tccctgataa aagaccatgt tgcccggcag cagtcaaaac
     6481 ttccccaatc atagtcgttg tggttgtctt accgttcgat cctgtgatac caataatcgg
     6541 tgcttctgaa atcaaataag ccaattccac ctcagtcaag actggaattc ccttggccaa
     6601 agccttttca atcatgggat tgttgtaggg gatacctgga tttttcacca taagggcaaa
     6661 ctcttcatcc aagagttcca aaggatggcc acctgtaatg accttgatcc cttcttccag
     6721 caaacattgg gcagctggat tgtcctcgaa tggtttccca tcatttactg tcacaatggc
     6781 acctaacttg tccaacaaac gagccgcaga ttcaccagac ttggccaaac ctaaaacaag
     6841 aactttctta tttttaaatt gatctattac tttcatgtct cgaactccat ttctactcct
     6901 actattttac catttttatg gaaataaaaa agccacaaag tgtgtttgtg actctttctt
     6961 ctaactgaat cttaccatat catctatgtg ataaatcggt aactcgaatg acctgatcca
     7021 cttgctccca aatcagagga ttgtgggtcg caataataat ggtccgattc ggatttttta
     7081 aagattctag gatggaaagt aattcctcag aatttttggg gtctaaggaa gcggttggtt
     7141 catctgcaag aatcaaaggc ggatccttta aaattatctt cgctagtgca acacgttgtg
     7201 cttctcctcc tgataactca aatataggtt gcttcaaatc caaataagag aggtttacac
     7261 agtttagagc ttgtttcatc aaagagattt tctctttttc cttcaacttt ttaccaacta
     7321 aactcagatt gagattctct ttgacggttt ggctttcaat taagccaaaa tcttgaaata
     7381 agtatcctaa gtaatctcta aagaaaacag aaggcttgat gtccttaaga gaagtgccat
     7441 catagatgat ttgccctttg tcatatggct ccaatcgtcc aatcatattc aagagtgttg
     7501 tcttaccaca gccacttgta ccgattaagg cataaatttt cccaccttca aaatgaagat
     7561 tcgtatctga aaatagctga cggcttccaa attttttaga tatattcttt agttcaatca
     7621 tcctattttc ctttcataat tgtcatagaa acacgagatt ctttctgcgc ttgacggtaa
     7681 agcgtcaaaa ctgcactagc tagaaagacc aataaagtga gcaagccaat caccaagtct
     7741 cgactgctta aaataaagag actagcacca aatacaaaac tggcaaattg gctaaccata
     7801 tactgagcat gtgtttcaaa aaatcgtaaa cctgaaattc gtttaatcaa gatatctcgg
     7861 cggaattgct cgaaatatag aagattgaca gaataaaata gtaacaagga actagctatc
     7921 cccacaatag ctcctaagat taaagttgct gtttcagttt gaacttcatt ataacgagtt
     7981 agataaacac ttcttccttc tttaagatag gatacttgct cataaattcc agctttcttc
     8041 aagagttcta gcccactctc atatcctttg ataaagagtt gttttccagc attgatagac
     8101 caactagata aggatataaa actatcacct gtagaagtcg gcgtgaatac cactaaaatc
     8161 ggatcagtca aatactgagt ggatacggga ttctcaccgt tattataaac aaaccgcttt
     8221 tctcccattg aaagataact aacgtgcgct ttcatctcat aatccaaagg agcacttgcc
     8281 tcctcaccag attttccata ataactcaat ctttcttcaa aaactttctt aagttctgct
     8341 tctcgagagc gcaaatgttc tgggagcaag aggataaact cacctttttg gagatgggct
     8401 aacttctgtt tggtctcagc atctaccacg accttttcct tgtccaaata actgggacta
     8461 acatagagcg tattagcatc tggactatag gtatccagtg tctctccctg ttcatttttt
     8521 ccttgtggat tggcaaaatg gagcagatta tcctttacat aaagagcttg ttcttcttcg
     8581 attgcttcct tggcaaaggc ataccacttg ctctgatttt ctgtatcttt tcctctatca
     8641 cctaagccaa aagaaatttg ataatagtct gctctatcct gccatgcttg ttttgaaatt
     8701 tcaagttctt tcaatcgttg gtaagacatc aaacctgtct taacagcgta gcctactgta
     8761 aagacagcaa ccaactgaca caatagggtt aaaatcatca ggcgtttaag gggtaatctc
     8821 cccttaataa cgggaactaa tgctttgtaa ctcaaactca ttagataaag aagcattagt
     8881 aaaattgaaa tcgccaataa aaacaacaga tagaaactaa tcccaaaacc ataggtggct
     8941 aacaagatag gataaaacaa accttgacta aaaagaacga ctcccccacc taggaaggaa
     9001 aggagggctg atagaaggag ccatttgata tcagtagata aagaatgccc catgatggat
     9061 aagagagtct gaccagaaaa gagttttata cctgctgctc tcatttcctt aatccgagtg
     9121 ataatcacta aagcaaagaa agataagcca aatattgcta aactaattaa aataagggga
     9181 tttagtaata ttcgaaaagc aagaaaatag ggcggtatct ttcggtcagc acttgcttta
     9241 taacccaaat ctcctaattt atcggcaagc ttttctttcg tcaaggagcc tgacaaaagg
     9301 agataactat ttagcggatc actacgttca cgactttctt ggctagcttc ttggaattct
     9361 tttggtaaag ttccctgacc ataagttgca taagtaaagt gagtcgtccc atccttactc
     9421 ggctctacaa ttcttctagc tattaaactc tgttctgagt ttgcaaaatt ctccaattcc
     9481 tgttcaaata cctcacgcgt cggttcctga gtatcttttt tgacacgaag taaagaaacg
     9541 gaatcatagc ttgcatataa atattgtggc gcacgtaaga caataatcca agcaaggaag
     9601 aagctgagaa aaaaagttga taataatatg aatagtttct tcatagtaga ctccttgtaa
     9661 acaaaattcc ccctgtaatt tcttacaagg ggaacgattt aaatcaatga acgattagtc
     9721 ataatcacag taaaatgcta cttgttctcc ccatttagtc caaatccatg cataggaggt
     9781 ttttccagca gaagcttctc ctttatctga atgactattc catctactta caagagaaga
     9841 ataatgatat tttttaccat gatagtaatt tgaaaaagcc caaccacctc ctgaaccttc
     9901 tccatacgtc catactcctc catctggata ttgtacagca gctgatgcag ctcccaataa
     9961 tgtaaaactt gaaataagag ctagagcaag taatctatgt tttttcgttt tcattttatt
    10021 ttttctttca aaaaaagcac accttgagca acaatgcaac aaaataaacc ctcctctctc
    10081 ttttgttgaa accgctttct tatgtgataa aaatatcatt tttattattt gttgtcaagg
    10141 aaaaaatcga attttttaga t
//
LOCUS       DAVLYV010000073         9722 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLYV010000073 DAVLYV010000000
VERSION     DAVLYV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9722
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.073"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..1203
                     /locus_tag="VYO70_001690"
     CDS             <1..1203
                     /locus_tag="VYO70_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEV1713890.1"
                     /translation="STLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTI
                     ISWNSLRFQPYFTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHS
                     SRSPFNSKEPWFFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYP
                     ENNESYNVPNQYFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGV
                     KLSVYRDISTTPVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNG
                     QRYKTRLSIDWEMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEW
                     KDNKRTSLNDEVFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGSLL
                     EMIFTSQN"
     gene            complement(1425..1901)
                     /locus_tag="VYO70_001691"
     CDS             complement(1425..1901)
                     /locus_tag="VYO70_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1713891.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFSKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            2124..5327
                     /locus_tag="VYO70_001692"
                     /pseudo
     CDS             2124..5327
                     /locus_tag="VYO70_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
     gene            5675..6051
                     /gene="tnpA"
                     /locus_tag="VYO70_001693"
                     /pseudo
     CDS             5675..6051
                     /gene="tnpA"
                     /locus_tag="VYO70_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148688.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(6439..7068)
                     /locus_tag="VYO70_001694"
     CDS             complement(6439..7068)
                     /locus_tag="VYO70_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1713892.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(7078..8079)
                     /locus_tag="VYO70_001695"
     CDS             complement(7078..8079)
                     /locus_tag="VYO70_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1713893.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(8110..8751)
                     /locus_tag="VYO70_001696"
     CDS             complement(8110..8751)
                     /locus_tag="VYO70_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1713894.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFDRLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(8770..9585)
                     /locus_tag="VYO70_001697"
     CDS             complement(8770..9585)
                     /locus_tag="VYO70_001697"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1713895.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT         3041 a         1743 c         2096 g         2842 t
ORIGIN      
        1 agtacacttt ggcttttaaa ccattatcac tatcaagata gttgtaaaaa tgcagaaggt
       61 ggtttgattt tatcaagata tgcaggtcct ggtagtcacc gctaccctgt tggtttttca
      121 ggggatacta ttattagttg gaattcctta agatttcaac cctattttac agcgacagca
      181 tctaatatcg gttatagttg gtggagtcat gatatcggtg gacatatgtt gggggattat
      241 gacgaagagc tacaaactag atggctacag tttggtgttt ttagtccgat tactcgatta
      301 catagttcta gaagtccttt taatagtaaa gaaccttggt tcttttcaga aacaacatct
      361 aagattatga agaaatacct tcgtttgaga catcagatga ttccctatct atataccatg
      421 aatgtaaaga cacatgagga aggtgcccca ttaatcagtc caatctatta tttctatcca
      481 gagaataatg agagctataa tgttccaaac caatactttt ttggaacaga actgatggtg
      541 gctcccattg tagaaaagat ggatttgaca ttccaatctg caaaagtaga tgtatggttc
      601 cctgaaggtg aatggtatga cttcttttca gagaaaaaat acacaggtgg tgtgaagtta
      661 agtgtttata gggacatctc gactacgcct gtgtttgcaa aaagtggtgc aatcattccc
      721 ttggttggtt ctgagatagg tatgggtgtt gatttacctg aagttgtaga ttggtatgta
      781 ttcccaggga aacaacattc ttttgaaatg cttgaagatc aaaatggtca aagatataaa
      841 acaagattat caatcgattg ggaaatggga atggtagagt tagcattaca aggagattct
      901 agtatcgttc caagcaatag aaaacataga attcatttta aaggaacgaa tgtgtctata
      961 attgaattgc caaataagaa tgatacagct aaatttgaat ggaaagataa taaaaggaca
     1021 tctttaaatg acgaagtttt tagactacta aagacagctt ctcttccata tgaattaaaa
     1081 gatagattgt taaatcaatt catcaatgcg aaaaactctc atgacttaat gaatatcttg
     1141 catcatcagg ataaggaatt gagagggagt ttgttggaaa tgatatttac tagccaaaac
     1201 taattgaaat aattagaaag tcatttgttt agtgtatatg aagattctgt atggtattcg
     1261 ttatcactgg tcacattgaa aatgtgaaat aagcacctac aacatagact ttacaacact
     1321 tcatgtttca tatcatgggg tgtttttcta tttaagtcaa agcccatcaa aaaatctcac
     1381 tattcatcag gttaaaggaa acctaatcat agtgagattg tgaattatag tagagtttgt
     1441 atcgcctctt caatttgttt tgggtctgtt tttgaagaaa agcgttcaaa gacttgccca
     1501 tctcgaccga tgagaaactt agagaaattc cattcgattc gttttcctag tgggccagat
     1561 ttctggtctt ttaaccagac atagagaggg tctgcttcct taccgttgac cttaatcttg
     1621 gcaaaacgtg ggaaggtggt ttgaaaatgt aggctacaga aggcgttgat ttcctctgcg
     1681 ctgccgggtg cttgtcccat aaactgattg caagggaaat ccaatatttc gaagccctga
     1741 tcttgatagc gttcatagag ttcttgaagt ccctggtact ggggcgttaa accacatcca
     1801 gtagcagtgt tgacaatcaa gagaactttg ccacgatagc tatccaaggg agttgcttgg
     1861 ttattttggt tcaaaacgga aaaatcataa agtgaagtca tgagggcctc ttttcttttt
     1921 ctacattgta acagttttta cttttttcgt cgagtcggat ggaagatatt gttgaagaat
     1981 aagccctcaa tatgatagaa accgtttaca aataacttta aaaatggtat gatttaatta
     2041 aaccggttta cttagttgct ataaatctct aatttgagat ttagttccta ttgattttac
     2101 aatatgttta ttggagtgta tacatgcaaa caaaaacaaa gaagctcatt gtgagtttgt
     2161 cttcacttgt tttatcagga tttttattaa accattatat gacagttgga gcggaagaaa
     2221 cgactacgaa taccattcag caaagccaga aggaagttca gtatcagcaa agggatacaa
     2281 aaaatttagt tgaaaatggt gattttagtc agacggagga cggaagcagt ccgtggacag
     2341 ggagcaaagc tcaggggtgg tcagcttggg tagaccagaa gaatagttct gcagatgcct
     2401 caactcgagt cattgaggct aaggatgggg ctatcactat ctcaagccat gagaaattaa
     2461 gggcagtggt tcaccgtatg gttcctattg aagctaagaa aaagtataaa ctgcgtttca
     2521 agattaaaac agataataaa gtcgggattg ccaaagttcg tatcattgag gaaagtggta
     2581 aggacaagcg attgtggaat tctgcaacga cgtcaggaac aaaggactgg cagaccattg
     2641 aagcagacta tagcccgact ttagatgttg ataaaatcaa gctggagtta ttctatgaaa
     2701 caggaactgg gactgtttcc tttaaggata ttgagctggt agaggtagca gaccagcttt
     2761 ctgaggattc tcaaacagat aaacagcttg aggaaaagat tgatttacca attggaaaaa
     2821 aacatgtttt ttctcttgcg gactatactt ataaggtaga aaatcctgac gttgcttcag
     2881 tcaaaaatgg aattttagaa cctcttaagg aagggacaac caatgtcatt gtcagtaaag
     2941 atggcaagga agtgaaaaag attcctttga agattctagc ctctgttaag gatgcataca
     3001 cagaccgttt ggatgactgg aatggcatca tcgctgggaa tcaatactat gattctaaaa
     3061 atgaacagat ggccaaatta aaccaggaat tggaaggaaa ggtagctgat agcctatcta
     3121 gtatttcaag ttaggcggac cgcacctatt tgtgggaaaa attttcaaat tataaaatgt
     3181 ctgcaaatct gactgccact tatcggaaat tggaggagat ggccaagcaa gtgaccaatc
     3241 cttcttctcg ttattatcaa gatgaaactg tcgttcgaac agtcagggat tccatggaat
     3301 ggatgcataa acatgtctac aatagtgaaa agagcattgt tgggaactgg tgggattatg
     3361 aaatcggtac acctcgtgcc atcaacaata ccttgtctct gatgaaagaa tacttctctg
     3421 atgaggaaat taaaaaatat acagatgtga ttgaaaaatt tgtaccagat cccgaacatt
     3481 tccgaaagac gactgataac ccatttaagg ctctaggtgg aaacttagtt gatatgggaa
     3541 gagtaaaagt aatagctggt ttactgcgta aggatgatca agaaatttct tctaccattc
     3601 gctcgattga gcaagtgttc aagttggtag accaaggtga aggtttttat caagatggat
     3661 cctatatcga ccataccaat gttgcctata cgggtgctta tgggaatgtt ttgattgatg
     3721 gcctgtctca actgttgcca gtcattcaaa agaccaagaa tccaatcgat aaagataaaa
     3781 tgcaaaccat gtaccactgg attgataaat cgtttgctcc tttgctggtg aatggagagc
     3841 tgatggatat gagtcgtgga cgctcgatca gtcgtgcaaa tagcgagggg cacgtggccg
     3901 cagtagaagt actaagaggg attcaccgaa tagcggatat gtctgaagga gaaaccaaac
     3961 aacgtttgca gagtcttgtg aagaccattg ttcaatcgga tagttattat gatgtcttta
     4021 agaatttgaa gacttataag gatatcagtt tgatgcaatc cttgttaagt gatgcaggag
     4081 tcgcaagtgt tccaagaaca agttacctat ctgcctttaa caagatggat aaaacagcca
     4141 tgtacaatgc agagaaaggg tttggatttg gcttgtcact cttttccagt cgtaccttga
     4201 attacgaaca catgaacaag gaaaataaac atggttggta tacgagtgat gggatgttct
     4261 atctttacaa tggcgatttg agtcactata gcgatggcta ctggccaaca gttaatccat
     4321 ataagatgcc tggtacaaca gagacggatg ctaagagagc ggatagcgat acaggtaaag
     4381 ttctaccgtc tgctttcgtt ggaacgagca aactagatga tgccaatgcg acagcaacca
     4441 tggatttcac caactggaat caaacattga ctgctcataa gagctggttt atgctgaagg
     4501 ataagatcgc ctttttagga agcaatatcc aaaacacttc aacagatact gctgcaacta
     4561 caattgacca gagaaaactg gaatcaagta atccatataa agtctatgtc aatgataaag
     4621 aagcctccct tacagaacaa gaaaaggatt atcctgaaac ccaaagtgtc tttttagaat
     4681 cgtccgattc gaaaaagaat attggttact ttttctttaa gaagagttca atcagtatga
     4741 gtaaggcttt gcaaaaggga gcctggaagg atatcaatga aggacagtca gacaaggaag
     4801 ttgaaaatga atttcttacg attagtcagg ctcataagca aaatggagat tcttatggct
     4861 atatgctcat ccctaacgtg ggtcgtgcca ccttcaatca aatgataaaa gagttagaaa
     4921 gtagcctcat cgaaaataac gaaacccttc agtctgttta tgatgccaaa caaggagttt
     4981 ggggcattgt gaaatatgat gattctgtct ctactatttc caaccaattc caagttttga
     5041 aacgtggagt ctataccatt cgaaaagaag gggatgaata taagattgcc tactataatc
     5101 ctgaaaccca ggaatcagct ccagatcagg aagtctttaa aaagctagag caagcagctc
     5161 agccacaagt acagaattca aaagaaaagg aaaaatctga agaggaaaag aaccattcgg
     5221 atcaaaagaa tctccctcag acaggagaag gtcagtcaat cttggcaagt ctagggttct
     5281 tgctacttgg ggcgttttat ttattccgta gaggaaagaa caactaattt gttcatcatc
     5341 tagatgaatt acatgaaact gtggaaaata gaaaaaatcc gaagaaggcc aaatatcttc
     5401 ggatttttct tttaaaaaag ataagttgat acttatcttt tttacttcat tatgtgacta
     5461 tagcgttttg aaactagaat aggacgtcat aactgctaaa agatttctat aaattcattt
     5521 gattttccta atcaatttgt tcgtatccta tttcactcca ctatattagt ccgtctcgct
     5581 ccgtgaggtg cgcgtcgaag gttataccaa aaaaactcca aacgcgatac aataaaggtg
     5641 ttcaatccaa ttgtaaagcg aaaggagaaa aaatatggca caaaaggctc atagtttatc
     5701 tcacacaaag tggaggtgtt ctatcacatt gtgttcaccc ctaagtatag acgaaaagtc
     5761 atctataatc aatatagaag tagtttaggc gaaatatttc atcgcttgtg tagttataaa
     5821 ggtgttgaaa tgatcgaggg tcacttaatg ccagaccatg tacacatgtt agtcagtatt
     5881 ccgtcgaggc taagtgtttc aagttttatg gggtatttaa aaggcaaaag tgcactcatg
     5941 atgtttgaca aacacgccaa tctcaagtac aagtttggga atcggcattt ctgggcggaa
     6001 ggttattatg taagtccagt agggcttaat gaagccacaa ttaagaaata tagtcaagat
     6061 taaagaaatt atcgagtgga tgatttcttc acgagtatga aaatttgaga acgagtaaag
     6121 catgatatat agtaaaatga aataagaaca ggacaaaatc gatcaggaca gtcaaatcga
     6181 tttctaacaa tgttttagaa gcagaggtgt actattccag tttcaatcta ctataacact
     6241 agataaattg agtgtaaaag aatatgagga tccctttagg gatagtggta agtaatacca
     6301 aagtctctta aagaggcaag tgacgagtca agagcaataa ggcttgaaca acgtgaaagc
     6361 cagcgtcttt aggcgctggc tgatgatttg ggcttatagc tctgagataa accacccgtt
     6421 agacgggtag ttatgatttt atctgagtgt aatatactgt tgggcaatct cgctgatgcg
     6481 gtcaaagttg ccttgggaag cgagtttatt gagttcgcca ccaattccaa cggcatctgc
     6541 accagcagcg aaccattgag ggatgttgtt taggccgact cctccggtta ccattacgga
     6601 aacttgtggg atcggtgcct tgactgcaga gatatatgct ggactgagag tactacctgg
     6661 gaagagtttg atgatttcac taccggcttc aagtgcagtc gtgatctctg tgagggtaat
     6721 acagcctgga atgtacggtg tgctgtagag attgcacatt ttcgcagttt cagcatggaa
     6781 agatggagaa acaacgtaat ttgctccagc tagaatggca tctctagcag ttacggcatc
     6841 aagcacagta cctgcaccga tacaaacact ctgatcgtcc tgatacaagt ctacaagttc
     6901 cttgatgatt tgtcctgcat actgattggt ataggcgatt tcaatagctt tgataccgcc
     6961 cttgatacaa gcaatcgagg cttgtagtcc ttcttccttt gtatttcctc gaatgacagc
     7021 gacaattttc tgttttttta gttcaataat cgtatctgat ttggtcatgt aattctccta
     7081 acgaatgata tcttgtgcat ttgccagtaa attttcaata ctagttgcgg aagtggagag
     7141 atggtctcct ggaagagtgc atttgagagt tgcgctcgca actgcaaagt caatggtagt
     7201 ttttagggag gaatgatgga gtagttggta aagggcacca gatataaagg catccccgct
     7261 accaattcgt tgatagactg cagtttttag ttggacagac tcttcaaatc gttcttccag
     7321 agcataggct tgatagacat ttttgtcttg ctcatcacta gagcggaggg tatggaaaat
     7381 ggccttgaaa ccataggctt cttttaaaag tcgcatgcga ttttccacct cttctaggct
     7441 agcactgtct cttggaaaca tctctagatt ttggtcatca atcatgagag gatcaatacc
     7501 gaagcaatag tcagtaaaac gtgcaaactt agaaaattca tacttggctt ctaggactga
     7561 aatcatcttt gttctcagat tgagatccat tgaaacgaca attcctcggc gcttggcttc
     7621 ttccaagagt aggagaagga tcgcacggac ctcttgaccg atagctacgg tgattccact
     7681 aaaatgaaaa tggctaatcc cctgaaagag agaatccata tctagcatgt ttggccgaat
     7741 ctggctgata ctcgtatgct tacgatcgta gaaaacttca ctttgacgac aaccaaagcc
     7801 gttctccaaa tagtagaggc cgattcgatc gccaagccga cagattgaac tggtatcgat
     7861 ttggtgctgt ttcaagaatg tgagaaaacg atctccaatc tcgttggcag ggagtgcggt
     7921 aaagactttc gttgagatac ccagggcttg caaattacaa gcgatgttaa tttctgatcc
     7981 gccaaaataa gtcgaactgg caacatgatc gccgatactg gtggcatcta atggtgagat
     8041 tcgaattaat ggttcgccaa acagtaatat tttcgtcatc cattagttcc tttctggttt
     8101 agaatagctt tacttggcta atacttcttt caaataagca gcgacttcat catcttggca
     8161 gttttcaaag aagtattctt ggaaatattt gccagagatg gtgtctttga ggaagtcttg
     8221 gtcgattgtt ttaaggatgg tcatcaaatc attgtgggtg atttgtttca cctcgtttaa
     8281 gatacgagcg ttgcgttgtt cagggattac acgttctctt gggtagccac cgcccatttc
     8341 ttcagcaaat aagcgatcaa acatcaattt gagggtcagt tctgcccccc agccaaatcc
     8401 tttggcataa gggatagaca aggcgttacc accattgatt tgagaataaa ggtaagcgtc
     8461 agttgggtcc actgctagac cacagacaac accagggaag ctgtttaaag caagcatagc
     8521 ccctacaccc gtaccacagc cggtaacaac aaagtcaact gcctttgtat ttaaaaggat
     8581 ggcagccatt agtccgttct gcacataagt taattgactt tctccttctt ctccacgcat
     8641 accatagtta aataattggt agtctttttt atctgtcgct tcttttagac tatcgtaaat
     8701 aatgtgattc ttgctagctt gactattttc attgattaat gcgattttca taagatcttc
     8761 tttctatttt tactcaggtt gttttccgat gtaggctaag ataccgccat ctacatatag
     8821 gatgtggccg ttgacaaaat tgctggcatc actagcgaga aagacagcag ggcccatcaa
     8881 atcttcagga tttccccaac gtgcagcagg tgtttttgca atgatgaact ggtcaaatgg
     8941 gtggcgagaa ccatcttctt gcaactcacg aagaggtgct gtttgaggag tggcaatata
     9001 acccggtcca attccgttac attggatatt ggctccaccg tattcagacg caatgttgcg
     9061 ggtcaacatt ttcaagcccc ctttagcagc agcataagcg ctaactgttt cacgtcccag
     9121 ttcgctcatc atcgaacaaa tattgataat ttttccatgc cctttcttta tcatagaagg
     9181 aataactgcc tttgaaacga taaatggtgc gtttaagtca atatcgatga ccttacggaa
     9241 atcagcggcg ctcatttcgc acattggaac tcggcggata ataccagcgt tattaacgag
     9301 gatgtcaatg acaccaacct cttgttcgat ttgcttgacc atggcttgga taccgtcctc
     9361 gtctgtcacg tcacagacat atccatgggc ttcgatgcca acttcacgat aagcttcaat
     9421 ccctttattg accagatctt gattgatatc gttaaagaca atagtagcgc cggcttcagc
     9481 gtaggatttg gcaatagcaa atccaattcc ataagaagcg ccggtgatga gagcaatttt
     9541 tccttttaaa gaaaactgct caattgagaa tgatgtattt gtcatttgat acttcctttc
     9601 aaatttgtta agacttctct ataatcaaat tatattctag taaaccggtt ttgtcaataa
     9661 ataaatattt ttatttataa aaaggtttgt tttcctaaaa aaacggaaga ttttgaaatt
     9721 tc
//
LOCUS       DAVLYV010000074         9627 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLYV010000074 DAVLYV010000000
VERSION     DAVLYV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.074"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(34..231)
                     /locus_tag="VYO70_001698"
     CDS             complement(34..231)
                     /locus_tag="VYO70_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1713896.1"
                     /translation="MTLNEYILQYRLKQAIDKMAESPNSPLSAISDQVGFSDYKYFAK
                     VFKKYLHISPKKLKSLGRIVK"
     gene            complement(306..1082)
                     /locus_tag="VYO70_001699"
     CDS             complement(306..1082)
                     /locus_tag="VYO70_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HEV1713897.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGVNALHPEDLIDYQKVFLNHHINCYF"
     gene            1204..1950
                     /locus_tag="VYO70_001700"
     CDS             1204..1950
                     /locus_tag="VYO70_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1713898.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     RQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1963..2943
                     /locus_tag="VYO70_001701"
     CDS             1963..2943
                     /locus_tag="VYO70_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1713899.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3138..3458
                     /locus_tag="VYO70_001702"
     CDS             3138..3458
                     /locus_tag="VYO70_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1713900.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            3504..3812
                     /locus_tag="VYO70_001703"
     CDS             3504..3812
                     /locus_tag="VYO70_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1713901.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQASGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            3829..5127
                     /locus_tag="VYO70_001704"
     CDS             3829..5127
                     /locus_tag="VYO70_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1713902.1"
                     /translation="MAKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLLSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSWYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5278..7716
                     /locus_tag="VYO70_001705"
     CDS             5278..7716
                     /locus_tag="VYO70_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1713903.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIGGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKAALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            7735..8403
                     /locus_tag="VYO70_001706"
     CDS             7735..8403
                     /locus_tag="VYO70_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1713904.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHKIRRQAGDDIFIKVPVTPAGLRAIKALK
                     KEGYHITATAIYTVIQGLLAIEAGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            8421..9509
                     /locus_tag="VYO70_001707"
     CDS             8421..9509
                     /locus_tag="VYO70_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1713905.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPITLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
BASE COUNT         3191 a         1338 c         1939 g         3159 t
ORIGIN      
        1 tagagtaaaa ttctctacaa aaagaagaat agcctatttg actattcttc cgagtgattt
       61 caatttcttt ggggaaatat ggagatactt tttaaatact ttggcaaaat atttatagtc
      121 tgaaaatcca acttggtcag agatagcgct taaaggggag ttgggagatt cagccatctt
      181 atctatagct tgtttcaggc gatattgtaa gatatattca tttagagtca tggggaagtc
      241 ttctttgata accttatata gatgcttgtt atctgcaaat agctagagca tttcaaggaa
      301 atgaactaga aataacaatt aatatggtgg ttcagaaata ccttttgata atcaataaga
      361 tcttcaggat gaagtgcgtt gactccatcc atttcatatt tccgatttaa taaacgatat
      421 ttgttttcac caaattgatg aaaagggagt agttgaactt ggtcgatatt taatgagtta
      481 aatagagtag cgaatttttc tgcatcctct aaactattgt taaaattagg aataactggg
      541 attcttaaaa cgatagtttt attttgagaa aaagcataat gaatgttttt aataatcatt
      601 tgattaaaaa ccccagtcac ttttttatgt tttatagaat tataatgttt taggtctgtg
      661 tagataaaat ccacatattg aattaaatca ataaattttt catgatcaac aaaggcagta
      721 gtttcaatgg cagtgtgtat gtgatgttct ttagctgatt ttaagatggc tttagcaaat
      781 tcaaactgag caaatatttc acctcctgat aaagttaaac ctccaccgga ttcttcgtaa
      841 aattctttgt cttttaatac ctctgtaata atttcttcta cagtcttttc ttcaccgact
      901 aaggtgaatt tctctcgttg agcatctttc attttttcag gtttcattct ttgagattca
      961 ggattagaac accatggaca gcgcagagga catcctttta aaaaaacagt tgtacgaata
     1021 cccgggccgt catgaattga aaagtgttga atattaaaaa taattccttt agatatttcc
     1081 ataagagttc ctcctcttca taagtctatc ctatcacaaa aacgaaagaa aaacaattac
     1141 gaacgaaagt ttaatttgtt gttttttgct ttttattttg gtagaataaa acggaggtgt
     1201 aaaatgaaaa gattggaaca aattattaaa ttagtatcag aatatgaaaa gatcgacgtt
     1261 aatacattat cggaaaaatt aaatgtatcg aaagtaacga ttagaaaaga tttagataaa
     1321 ttagagtcaa aaggtttatt acacagagag catggatatg ctgtattaaa tagtggagat
     1381 gacttaaatg tacgtttgtc aattaattat gaaattaaga gaaaaattgt tcaggaagca
     1441 gtaaaattgg tgtcagataa tgaaacaata atgatagaat ctggatcgac ctgtgcttta
     1501 cttgctgagg aaatttgcaa gcaaaaaaga aatgttacga ttgtaacaaa ttcatttttt
     1561 atagcaaatt ttgtgagagc ttatgattca tgtcgtatta ttgttcttgg tggagagttt
     1621 cagaaggatt cacaggttac tgtgggacct ttattaaaag aaatgataca gacttttcat
     1681 gtgcgtcaag cttttgttgg gacagatggc tacgataaag agatgggctt tacaggaaaa
     1741 gatttaatgc gcagtgaggt agttcaatat atttcagcag cgtcggataa agtcattgta
     1801 ctaactgact caagtaaatt tgataaaagg ggtacagtaa gaagatttgc tttaagtcaa
     1861 gtctatgaag taataacaga cgaaaaactt tctaaacaaa atatagctac attagaaaat
     1921 gctgggataa tggttaaggt agtttcgtaa gaggttaagt gtatgaatca agataggaat
     1981 aaactgcttt ctaaaattgc ttatctgtat tatattgaaa acttaaatca gtcacaaata
     2041 gcagcaaaat taggaattta tagaacctct attagtagaa tgttaacaga agcaaggaat
     2101 gcaggaattg ttaaaattga aatagagaat tttgatacaa atatgtttaa gttggaaaat
     2161 tatgtaaaag aaaaatatgg tctggaaagt ttagaaatta ttccaaatga atttgatgat
     2221 aatccaacaa ttttatctga aagaatttct caagttgcag caggtgtcct taggaattta
     2281 attgatgata atatgaaaat tggcttttct tgggggaaaa gtttaagtaa tttagtagat
     2341 ttaattcaca gtaaaagtgt ccgaaatgtt cacttctatc ctctagcagg tggtcctagt
     2401 cacatacacg ctaaatacca tgtgaataca ctgatttatg aaatgtctag aaaatttcat
     2461 ggagagtgta catttatgaa tgcaacgatt gtgcaagaaa ataaattgtt agcagatggt
     2521 attttgcaat caagatattt tgaaaatttg aaaaatagtt ggaaagattt agatatagct
     2581 gtagtcggaa ttggtgattt tagcaataaa ggaaaacatc aatggttaga catgcttaca
     2641 gaggatgact ttaaagaatt gaccaaagtt aaaactgtag gagaaatttg ttgtcgattt
     2701 tttgattcaa aaggtaaaga agtctatgaa aacttacagg aaagaacgat agcaatctct
     2761 ttggaagatt taaaaaatat tcctcaaagt ttagctgttg cttacggtga tacgaaagta
     2821 tcttcgattc tttctgtctt gcgtgctaat ttagtaaatc atttgattac agacaaaaat
     2881 acaattttaa aagttttgga agaagatggg gatttgactt ttagagagat tctaggtgag
     2941 tgaaaatgat agactgatca gtttatcgtt ttttcttttt agtcgattgc acatttgtgc
     3001 tcatataaac aaaaatagtt tatctgttgt ttttggattg acaactttat tatgtagttg
     3061 tattctatag ttacaaaaga aaattttaaa atttcaaatg aaaaaagctt tttacatagt
     3121 gaaatgagga ggaatttatg gaaattattg ttccagatca aattatcatg ggtttaattt
     3181 tatatgctgg tgatgcgaaa caacatattt ataaagcgtt agattacata aaaaatggta
     3241 catgtgaacg gtgtgaagaa gaaatccagt tagctgatgc cgccttatta gaagcccata
     3301 atctacaaac aaaattcttg gcacaagaag cgtctggtac aaagacagaa attacagctc
     3361 tctttgttca ttcacaagat catctcatga ctagtatgac ggagattaat ttaatcaaag
     3421 aaattattag tttgagaaaa gaacttcata aaaaataata ctagagtatt atcattgtta
     3481 ttaacataga ggaggaaaac ataatggtga agattggttt gttttgtgca gcaggttttt
     3541 ctactggtat gcttgtaaat aatatgaaaa ttgcagcgca agctagtgga gttgaggcag
     3601 aaatagaggc gttttctcag tctaaattag cggattatgc gccaaatata gatgttgcac
     3661 tattgggtcc acaagttgct tatacattag ataaatcaaa agaaatttgt gataagtgtg
     3721 atgttccgat agctgttatt ccgatgatgg actatggtat gttagatgga aaaaaagtat
     3781 tagatttggc cctatctttg attagtgggt aagaaaagga gatttattat ggcaaagatg
     3841 gatgttcaga aaatcattgc accgatgatg aagtttgtga atatgcgtgg cattatagct
     3901 ctaaaagatg ggatgttagc aattttgcca ttgacagtag ttggtagttt attcttgatt
     3961 atgggacaat tgccgttcga aggattaaat aagagcattg ctagtgtttt tggagctaat
     4021 tggacagagc cgtttatgca agtatattca ggaacttttg ctattatggg tctaatttct
     4081 tgtttttcaa ttgcctattc ttatgctaag aatagcggag tagaggcttt accagctgga
     4141 gttctatctg tatctgcatt ctttattttg ctaagatcat cttatatccc taaacaaggt
     4201 gaggcaattg gggatgctat tagtaaagtt tggtttggag gtcaaggaat tatcggtgct
     4261 atcattatag gtttggtagt aggaagtatt tataccttct ttataaagag aaaaattgtt
     4321 attaagatgc cagaacaagt tccacaagct attgccaaac agtttgaagc aatgattcca
     4381 gcatttgtaa ttttcttact ttctatgatt gtatatattt tagcgaagtc attgactaat
     4441 ggcggaacat tcatagaaat gatttattct gctattcaag ttccattgca aggtttaact
     4501 ggatcttggt atggtgcaat tggaattgca ttctttatat catttttgtg gtggtttggt
     4561 gttcatggac aatcggtagt aaatggagta gtgacagctc tgcttttatc taatcttgat
     4621 gctaataaag ctatgttagc ctctggtaat ctatcattag aaaatggtgc acatattgtt
     4681 actcaacaat ttttagattc atttttaatt ctatcaggtt cagggattac gtttggtctt
     4741 gtagttgcca tgctttttgc agcaaaatca aaacaatacc aagccttagg aaaagttgcc
     4801 gcttttccag caatatttaa cgtaaatgag ccagttgtat ttggatttcc gattgtcatg
     4861 aatccagtta tgtttgtacc tttcattctt gttcctgtac ttgcagctgt gatagtatat
     4921 ggagctattg caacaggttt catgcagcca ttctcagggg taacattgcc ttggagtaca
     4981 ccagctattt tatcaggatt tttggtgggt ggatggcaag gagttattac tcagctggtg
     5041 atattagcga tgtctacatt ggtttatttt ccattcttta aagtacagga tcgtttagct
     5101 taccaaaatg aaatcaaaca atcttagagg tatttgtgtg ttactgttaa actcacacat
     5161 ttgtgctaaa aattagagag ttaaaatttt tctagttaaa agcttgaaaa tttctataaa
     5221 aatcggtatt atattttcga aagaaataaa aatattttcg aaagaaaggt ggttacgatg
     5281 gtaaatacag aagtagcaag aacaacaatc aagacagaat attttggcag ccttactgaa
     5341 aggatgaaca aatatcgaga agatgtttta aataaaaaac cttatattga tgctgagaga
     5401 gcagttctag caacacgcgc ctatgaacga tacaaggaac aacctaatgt cctaaaacgt
     5461 gcatatatgc tgaaagaaat tttggaaaat atgactatct atattgaaga agaatctatg
     5521 attgggggaa atcaagcttc ttccaataaa gatgctccta tttttccgga atatacgcta
     5581 gaatttgttc tcaatgagtt ggatcttttt gaaaagcgtg atggagatgt tttctatatt
     5641 acagaagaaa caaaagaaca acttagaagt attgctccgt tttgggaaaa taataattta
     5701 cgtgctagag ctggtgcctt attacctgaa gaagtgtctg tttatatgga aacaggattc
     5761 ttcggtatgg aaggtaagat gaattctgga gatgctcacc tagcagttaa ctatcagaaa
     5821 cttttgcaat ttggcttaag aggttttgaa gagcgggctc gtaaagcaaa agcagctcta
     5881 gatttaacag atccagcaag tattgataaa tatcattttt acgactctat atttatcgta
     5941 atcgatgcta ttaaagtata tgcaaagcgc tttgttgctc ttgctaaaag tttagccgaa
     6001 aatgcaaatc ctaaacgtaa gaaagaatta cttgagattg cagatatttg ctctagagtc
     6061 ccatatgaac cggcaactac ttttgcagaa gctattcaat cagtttggtt tattcaatgt
     6121 attttacaaa ttgaatctaa tggccactct ctttcatatg gccgttttga tcaatatatg
     6181 tatccatata tgaaggctga tttagaaagt ggtaaagaaa cagaagatag cattgttgaa
     6241 cgtctgacaa atctttggat taagacaatt acaattaata aggttcgcag tcaatcacat
     6301 acattttctt cagcaggaag tcctttatat caaaatgtta caattggtgg acagactcga
     6361 gataagaagg atgctgttaa cccattatct tatttggtat taaaatcagt tgcacaaacc
     6421 catctaccgc aacctaatct aactgtacgt taccatgcag gtttagatgc tcgtttcatg
     6481 aatgagtgta ttgaagtgat gaaacttggt tttggtatgc ctgcatttaa taatgatgag
     6541 attattattc cttcttttat tgcaaaagga gtattggaag atgatgctta tgattacagt
     6601 gccattggat gtgttgaaac ggcagttcca gggaaatggg gctatcgttg cacaggtatg
     6661 agttatatga acttccctaa ggttctactt atcacgatga atgatggaat tgatccggct
     6721 tcgggtaaac ggtttgcacc aagctttggt cattttaagg atatgaagaa cttttctgaa
     6781 ttagaaaatg cttgggataa aacactaaga tatttgacac gaatgagtgt tattgttgaa
     6841 aattctattg atttatcatt ggaacgagaa gttcctgata ttctatgttc agcattgact
     6901 gatgattgta ttggtcgtgg aaaacacctt aaagaaggtg gagcagtata tgattatata
     6961 tcaggattgc aagttggaat tgcaaatttg tcggattcat tagctgcaat taaaaaattg
     7021 gtgtttgagg aagaacgtat aagcccaagt cagctttggc atgcactgga aacagattat
     7081 gccggagaag aaggtaaggt cattcaagaa atgttgattc atgatgcacc taagtatggt
     7141 aatgatgatg attatgctga caaattggtt actgctgctt atgacattta tgttgatgaa
     7201 attgctaaat atcctaatac acgttatgga agaggtccaa ttggaggaat tcgttattca
     7261 ggaacatctt ctatctcagc caacgtaggg cagggacgtg gaacattagc aactccagat
     7321 ggacgcaacg cgggtacacc gttagcagag ggttgttcac catcacataa tatggatcaa
     7381 cacggcccta catctgtttt aaaatctgtt tcaaaattac caacagatga aatcgtaggt
     7441 ggggttctct taaatcagaa agtaaatcct caaacgttag ccaaagaaga agataaatta
     7501 aaactaattg ctttgttacg aacattcttt aatcgtttac atgggtacca tattcaatac
     7561 aatgttgttt ccagagagac gctgattgac gctcagaaac atcctgaaaa acacagagac
     7621 ttaattgttc gtgttgcagg atactctgca ttcttcaatg ttctttctaa ggcaacccaa
     7681 gatgacatta taggacgtac tgagcatact ttgtaaaata aagaggttct ttttatggaa
     7741 tttatgcttg acacattaaa tttagatgag attaaaaagt ggtctgaaat tttgccgcta
     7801 gctggggtaa cttcaaatcc cactattgca aaaagagagg gttctattaa tttttttgaa
     7861 cgaatcaaag atgtaagaga attgattggc tctacaccct ctattcatgt tcaggtgatt
     7921 tctcaagatt ttgaaggcat cttaaaggat gctcataaaa ttcgaagaca agcaggagat
     7981 gatatattta tcaaagtacc tgttactcca gctggattac gtgcaataaa ggcgctaaaa
     8041 aaagagggct accatatcac tgcaacagct atttatacag ttattcaggg attattagct
     8101 atcgaagcag gagcggatta cctagctcca tattataata gaatggaaaa tctgaacatt
     8161 gattcaaatt ctgtcattcg tcaattagct cttgctattg atagacagaa ctctcctagt
     8221 aagattttag ctgcatcctt taaaaatgta gcacaagtaa ataatgcttt agctgcaggt
     8281 gcgcatgctg ttacagcagg agcggatgtt tttgaatcag ctttcgccat gccatctatc
     8341 caaaaggcgg ttgatgattt ttctgacgat tggtttgtta ttcaaaatag tcgttccatt
     8401 tagatagaga ggaaatacat atgagaattt ttgctagtcc ttctagatat attcaggggg
     8461 aaaatgcctt gtttgaaaat gccaaatcaa ttttggattt gggaaattac cctattctat
     8521 tatgcgatca gttggtttat gatattgttg gaaaacgatt tgaagattac ctacataggt
     8581 atggtttcca tattgttctg gcgctattta atggtgaagc ttctgacaat gaaatcaatc
     8641 gagttgttgc cttggctgag aaagaaaatt gtgatagtat tatcggtctt ggtgggggaa
     8701 agacgattga tagcgcaaaa gctattgcag atttgattga aaagcctgtt attattgctc
     8761 caacaattgc atcgaccgac gcacctgtat ctgctttatc tgttatttat acagatgaag
     8821 gtgcatttga tcattatcta ttttattcta aaaatccaga tttagttttg gttgatacaa
     8881 aagttatttc acaagcccct aagcgtttat tagcgtctgg tattgcagat ggtttagcaa
     8941 cttgggttga ggcgcgtgcg gttatgcagg caaatggaaa aactatgttg ggacaacagc
     9001 aaacattggc tggagttgca attgcgaaga aatgtgaaga aacgctgttt gcagatggtt
     9061 tacaggctat ggcagcttgt gaagctaaag tggtgacacc agcattagaa aatattgttg
     9121 aagctaatac tttattgagt ggtctaggtt ttgaaagtgg aggattagct gcggcgcatg
     9181 caattcataa tggttttact gcattgacag gtgacattca tcatttaaca catggtgaaa
     9241 aagtagctta tggaacttta gtacaactat tattggaaaa tagacctaaa gaagaacttg
     9301 ataagtatat tgagttttac aaaaaaattg gtatgccaat aactctaaaa gaaatgcatt
     9361 tggatcaagt tggatatgat gatttaataa aagttggtaa acaagcaact atggagggtg
     9421 agacaattca tcagatgccg tttaagattt cgccttcaga tgttgctcaa gctattatcg
     9481 ctgtagatgc ctatgtaaat tcaaaataaa caataaggac tactgttttc caaatggtag
     9541 tcttttattg atccctgtat tgaattctat agcagattga aataggatga gaacaaatcg
     9601 attgggaaag taaaattaat ttctata
//
LOCUS       DAVLYV010000075         9485 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLYV010000075 DAVLYV010000000
VERSION     DAVLYV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9485
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.075"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            459..1502
                     /locus_tag="VYO70_001708"
     CDS             459..1502
                     /locus_tag="VYO70_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1713906.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            1670..3757
                     /gene="topA"
                     /locus_tag="VYO70_001709"
     CDS             1670..3757
                     /gene="topA"
                     /locus_tag="VYO70_001709"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1713907.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNNEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMAVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            3863..4222
                     /locus_tag="VYO70_001710"
     CDS             3863..4222
                     /locus_tag="VYO70_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1713908.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            4315..4947
                     /locus_tag="VYO70_001711"
     CDS             4315..4947
                     /locus_tag="VYO70_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1713909.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            4991..5647
                     /locus_tag="VYO70_001712"
     CDS             4991..5647
                     /locus_tag="VYO70_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1713910.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            5666..6760
                     /locus_tag="VYO70_001713"
     CDS             5666..6760
                     /locus_tag="VYO70_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1713911.1"
                     /translation="MNFQLAKYSLLKKFSENIGFTTPEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGIAVANSLAAVKNGAGRVEETINGIRERAGNAALEEIAVALNIRQDYYQ
                     VETSIVLTETINTSEMVSRFPGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYENI
                     TPELVGVKIPLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALADKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAITDGIDAQDRVLVTVENRDTETIFNATGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            6772..7808
                     /gene="leuB"
                     /locus_tag="VYO70_001714"
                     /pseudo
     CDS             6772..7808
                     /gene="leuB"
                     /locus_tag="VYO70_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            7805..8074
                     /locus_tag="VYO70_001715"
     CDS             7805..8074
                     /locus_tag="VYO70_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1713912.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            8078..8437
                     /gene="leuD"
                     /locus_tag="VYO70_001716"
     CDS             8078..8437
                     /gene="leuD"
                     /locus_tag="VYO70_001716"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1713913.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            8599..9167
                     /locus_tag="VYO70_001717"
                     /pseudo
     CDS             8599..9167
                     /locus_tag="VYO70_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            9261..9326
                     /locus_tag="VYO70_001718"
                     /pseudo
     CDS             9261..9326
                     /locus_tag="VYO70_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         2944 a         1660 c         2102 g         2779 t
ORIGIN      
        1 tataatttag tgtaattgag aaaggagaaa tgattgtgat tgatgttggc taggttatgt
       61 tcaatgattc ctaccgtctc aaatcttgtc agtaaggaaa aataaattct tcaaaagtag
      121 agattacaag gcttgtttaa gaaagaattc aaagaccttg acaaataaaa ataaaatggt
      181 tattataaga aatggtctaa aatagatgat gatacttttc gaaaatcttt ttacgtcagc
      241 tcagctttgc cttgctgtgt tttgagcaag ctacggttag cttccgagtt tgattttcat
      301 ttactagaaa tgaaactgat gagagatatc agtagacatt tgagtcagga tattatggaa
      361 aatgataaaa agagctcgtg agattggcat atcagactac taaagtattg agtttgttag
      421 gattttagcg actagttagc tgggaaagga agatatttgt gacaaataat aaactgtatt
      481 cgttgataga atttagaaat aaaatatatg aagaattaga actttccaga agtgatttag
      541 cgattttact atgtgccatg cttatcgcct ctatcggatt aaatatggat tcgactcccg
      601 tgattattgg agccatgtta atctctcctt tgatgacacc tattctggga gtggggctct
      661 ctctagctat atttgatttt aaattgttaa gaaaatcttt taaaatatta gctattcaaa
      721 ttcttgccag tctaatagct tcaacacttt atttttatct ttctcccatt tcgtatgcta
      781 gttcggagat tgttgctaga acctctccga ctatttggga tgttctcatt gcttttgtag
      841 gagggatagc aggtattatt ggtgctagga aaaaagagac caataatatt gttcctggtg
      901 ttgctattgc aaccgccttg atgcctcctc tttgtacagt aggttatgct attgcttctg
      961 ctaatctaaa atttatcata ggctcctctt acctattcct catcaattgt agctttattg
     1021 tcattgcgac ttatataggt gttaggttga tgatggttaa gaaacattat tttaaagata
     1081 atgaagaaga ctctaaaatg cgtaggattt tgcttctagt tgctgttttg ctgatgattc
     1141 cgagtttcat ctctgcaacg actttagtga gagaaacgtt gaaaaaagag tcccttaaga
     1201 aatttatatc agagcagttt caggggtata atattttgaa aaaaacctat tctaaaaaga
     1261 ctcataccct aaagctaacc atttcaggaa attatttgac agaagaagaa ctcgatatga
     1321 tttcaagtaa gagaggtgac tatggtttaa gtgatgtttc tgttcaagtt tcacaattgt
     1381 ctgattcaga acaacttagt aaggaagagc tggtggagta tttcttccag tatatcaagg
     1441 ataaggaagc aaaagaaaag gagaaagcca ataaatttga tacagagtct gaggagcaat
     1501 aatttcttga gaatagctgg tttctctcgt gagtcttcta tgtatatcaa aggaagactg
     1561 aggttttaag tataaaactt ttcttctgtt tatagtagct aagtcttgac agggttcaaa
     1621 aaatggttta cactttataa agtttattac tttgaaaagg tgtgatactg tggctacggc
     1681 aacaaaaaag aaaaaatcaa cagttaaaaa aaatctagtc atcgtggagt cgcctgctaa
     1741 ggccaagacg attgaaaaat atctaggcag aaactacaag gtattagcca gtgtcgggca
     1801 tatccgtgat ttgaagaaat ccagtatgtc cgtcgatatt gaaaataatt atgaaccgca
     1861 atatattaat atccgaggaa aaggccctct tatcaatgac ttgaaaaaag aagctaaaaa
     1921 agctaataaa gtttttctcg cgagtgaccc ggaccgtgaa ggagaagcga tttcttggca
     1981 tttggcccat attctcaact tggatgaaaa tgatgccaac cgtgtggtct tcaatgaaat
     2041 caccaaggat gcagtcaaaa atgcttttaa agaacctcgt aagatcgata tggacttggt
     2101 cgatgcccaa caagctcgtc ggatcttgga tcgcttggta gggtattcga tttcgcctat
     2161 tttgtggaag aaggtcaaga agggcttgtc agcaggtcgc gttcagtcca ttgcccttaa
     2221 actcatcatt gaccgtgaaa atgaaatcaa tgccttccag ccagaagaat actggacagt
     2281 tgatgctgtc tttaaaaagg gaaccaaaca atttcatgct tccttctatg gagtagatgg
     2341 taaaaagatg aaactgacca gcaataacga agtcaaggaa gtcttgtctc gtctgacgag
     2401 taaagacttt tcagtagatc aggtggataa gaaagagcgt aagcgcaatg ctcctttacc
     2461 ctataccact tcatctatgc agatggatgc tgccaataaa atcaatttcc gtactcgaaa
     2521 aaccatgatg gttgcccaac agctctatga aggaattaat atcggttctg gtgttcaagg
     2581 tttgattacc tatatgcgta ccgattcgac tcgtatcagt cctgtagcgc aaaatgaggc
     2641 ggcaagcttc attacggatc gttttggtag caagtattct aagcacggta gcaaggtcaa
     2701 aaacgcatca ggtgctcagg atgcccatga ggctattcgt ccgtcgagtg tctttaatac
     2761 accagaaagc atcgctaagt atctggacaa ggatcagctc aagctatata cccttatctg
     2821 gaatcgtttt gtggctagcc agatgacagc ggccgttttt gataccatgg ctgttaaatt
     2881 gtctcaaaat ggagttcaat ttgctgccaa tggtagtcag gttaagtttg atggttatct
     2941 tgccatttat aatgattctg acaagaataa aatgctacca gatatggctg ttggagatgt
     3001 ggtcaaacag gttaatagca aaccagagca acatttcacc caaccgcctg cccgttattc
     3061 tgaagcaaca ctgattaaaa ccttagagga aaatggggtt ggacgtccat caacctatgc
     3121 gccaaccatt gaaaccattc agaaacgtta ttatgttcgc ctggcagcca aacgttttga
     3181 accgacagag ttgggagaaa ttgtcaataa gctcatcgtt gaatatttcc cagatatcgt
     3241 aaacgtgacc ttcacagctg aaatggaagg taaactggat gatgtcgaag ttggaaaaga
     3301 gcagtggcga cgggtcattg atgcctttta caaaccattc tctaaagaag ttgccaaggc
     3361 tgaagaagaa atggaaaaaa tccagattaa ggatgaacca gctggatttg actgtgaagt
     3421 gtgtggcagt ccaatggtca ttaaacttgg tcgttttggt aaattctacg cttgtagcaa
     3481 tttcccagat tgccgtcata cccaagcaat cgtgaaagag attggtgttg agtgtccaag
     3541 ctgtcatcag ggacaaatta ttgagcgaaa aaccaagcgt aatcgcctct tctatggttg
     3601 caatcgctat ccagaatgtg aatttacctc ttgggacaag cctgttggtc gtgactgtcc
     3661 aaaatgtggc aacttcctca tggagaaaaa agtccgtggt ggtggcaagc aggttgtttg
     3721 tagcaaaggc gactacgagg aagaaaagat taaataagag gaggggcccg aaagtaaatt
     3781 tcaggctcct tttggttaga acttgacaaa atacatcatt ttatgcgaaa ctagaagaag
     3841 attattttaa ctaggagaag ttatgcgtct tatctatctg ataattggtt ttttatcact
     3901 ggccttggct attgttgggg ttgttttacc cttgttgccg acaacgcctt tccttttgtt
     3961 gtctattgct tgtttctcca gaagttccaa gcgtttcgaa gactggcttt atcatactaa
     4021 gctctatcaa acatatgtag ctgattttcg cgagactaag tctattacgc gtgaacgaaa
     4081 gaaaaaaatc atcgtctcta tctacgtctt gatgggaatt tctatttatt ttgcacctct
     4141 tttaccagtc aaaatcggtc tgggtgcttt gaccatcttt atcacttatt atcttttcaa
     4201 ggtcattcca gacaaagaat agttaaaaca gtagttattt gccttgataa aattgaaagc
     4261 atattcataa taatatgata taataaattt gaagtaatat tcaaggagaa tcaaatgatt
     4321 tacgaatttt gtgctgaaaa tgtgacttta cttgaaaaag cgatgcaggc tggagctcgt
     4381 cggattgaac tctgtgataa tctagcagtt ggtgggacaa cacccagcta tggagtgact
     4441 aaggcagcgg ttgaactggc agctaactac gatacaacca tcatgaccat gattcggcca
     4501 cgtggtggtg actttgtcta taatgaccta gaaattgcta tcatgctaga agacattcgt
     4561 ttgactgctc aggctggaag tcaaggggtt gtatttggaa ctttaactgc tgataaaaag
     4621 ttggataagc ctaatctgga aaagttaatt gctgcatcaa aaggaatgga aattgtcttt
     4681 cacatggcct ttgatgaact aagtgatgaa gatcaaccgg aagctattga ctggctcagt
     4741 caagccggtg tcactcgtat cctaactcgt gctggtgtgt ctggcgactc cttagaaaaa
     4801 cgttttgttc actatcacag aattttggag tacgctaaag gtaaaattga aattctacca
     4861 ggtgggggga ttgaccttga aaaccgtcaa acctttatcg accaggtggg ggtaacacaa
     4921 ttgcatggta ctaaggttgt tttttaaaaa atagaaagga actgctagct ttgggtagca
     4981 gttttcactt atgtttgaaa tttttaaatc ctatcaattt aatcaagaaa aggctcatga
     5041 ttatggtttt atagaaaata gcgaagtctg gacatatagt tgccagattt tgcaaggtga
     5101 ctttgtcatg actgtgtcca tcactgctga taatgtgaac tttcaagtct ttgaccaaga
     5161 gactggtgac ctctatcctc acgttcatat ggaaagcatg aggggaagtt ttgtcggaaa
     5221 tgtccgtgag gcttgtctgg agattcttta ccagattcgg aaggcttgtt ttgatgtgca
     5281 agattttatc tgtcatcaga ctaagcgtat catgactcaa gttcaggaaa agtatggaaa
     5341 ccagttggag tatctgtggg aaaaatcgcc tgatacagct gtattgcgcc atgaaggcaa
     5401 tcaaaagtgg tatgccgtct tgatgaaaat ctcttggaat aagctggaaa agggcagaga
     5461 aggacaagtg gaagcagtca acctcaagca tgaccaagta gctaatttgc tttcacaaaa
     5521 ggggatttat ccagccttcc atatgagcaa gcgctactgg attagtgtgt cccttgatga
     5581 tactttatca gatgaagaag tactggaatt gatagaaaaa agttggaact taacctctaa
     5641 aaaatgaaat attttaataa ttttcatgaa ctttcaattg gctaaatatt ctttactgaa
     5701 gaaattttca gaaaatatag gatttaccac accagaggaa tatggtgcca tcttcaaata
     5761 cctgattgag aatgtcaaga cggatcgtca gatcatctat tcgcctcact gtcatgatga
     5821 cctcggaata gcagtggcaa atagccttgc tgctgtcaag aatggtgcag gacgtgttga
     5881 agagactatc aatggtatta gggagcgagc tggaaatgct gctttggaag aaattgcagt
     5941 ggctctcaat attcgccaag attactacca agtagaaacc agtattgtcc taactgagac
     6001 catcaatacg tcagaaatgg tttctcgctt ccctggtatt ccagttccta aaaacaaagc
     6061 cgtcgttggt ggcaatacct tctcccacga atctggtatt caccaagatg gagtccttaa
     6121 aaatcctctc acttatgaga acatcacacc tgaattggtt ggtgttaaga ttccgcttgg
     6181 aaaattatct ggtcgccatg cttttgttga gaaactgaga gaattggccc tagattttac
     6241 agaagaggat atcaaaccac tctttgctaa gttcaaggca ctggccgata agaagcaaga
     6301 aatcacagat gcagatattc gagctttggt agctggaacc atggttgaaa atccagaagg
     6361 cttccacttt gatgatttac aacttcaaac tcatgcagat aatgacattg aagcgctcgt
     6421 tagcctagcc aatatggatg gtgagaaagt cgaatttaat gcgacagggc aaggttccgt
     6481 tgaagcaatc tttaacgcta tcgataagtt ctttaatcaa tctgttcgtt tggtgtccta
     6541 cactatcgat gcgataacag atggaatcga tgcccaggat cgggttttgg tcactgttga
     6601 aaacagagat acagaaacca tctttaatgc aacagggctt gattttgatg tgttgaaggc
     6661 ttctgctatt gtctatataa acgctaatac ctttgttcaa aaagagaatg caggtgagat
     6721 gggacgcagt gtttcttacc acgatatgcc tagtgtgtaa aggagaaggc tatggcaaag
     6781 aaaatagtag ctctagtagg agacggaatt ggcccagaaa tcatggaggc tggtttagaa
     6841 gttctggagg ctctagctga aaaacaggtt ttgactatga gattgacaga cgaccgttcg
     6901 gaggtgcaga tattgatgca gcaggacctc ccttacctga tgaaaccctt aaggcaagta
     6961 gggaagcaga tgctatccta ctagcagcta tcggtagtcc tcagtatgat ggagcagcgg
     7021 ttcgccctga acaaggcctg atggctctcc gtaaggaact caatctttac gctaatattc
     7081 gtcctgtaaa aatctttgac agtctcaagt atttgtcacc acttaaaccg gaacgaattt
     7141 ctggtgtaga ctttgtcgtg gtgcgtgaat tgacaggcga gatttacttt ggagatcata
     7201 ttcttgaaga gcgcaaagcg cgtgatatca acgactatag ctatgaggaa gtggagcgga
     7261 ttattcgcaa agcctttgaa attgcaagaa atcgcagaaa aatcgttact agtatcgata
     7321 agcaaaatgt tctagcgacc tcaaaactct ggcggaaagt agctgaggaa gtcgcacagg
     7381 atttcccaga tgtaaccttg gaacaccagc tggtagactc agctgctatg cttatgatta
     7441 ccaatcctgc taagtttgat gttattgtaa cggagaatct ttttggagat attttatctg
     7501 atgaatcaag cgtcttatct ggtacacttg gggttatgcc atcagccagt cattctgaaa
     7561 atggaccaag tctctatgaa cctattcacg gttcagcacc tgatattgca ggtcaaggaa
     7621 ttgccaatcc tatttccatg attttatcag tttccatgat gttgagagat agtttcggac
     7681 gttatgagga tgcagagcgt atcaaacgtg ctgttgagac aagtctggcg gcaggaattt
     7741 taacgagaga tataggaggt caggcttcaa caaaggaaat gacggaagct attattgcaa
     7801 ggttatgaag ttagacgaaa aaattactct agtccttttg atttggaatg tcatcatttt
     7861 cttgatttat ggcattgaca aattcaaggc aagaagaaga gcttggcgca tccaagagaa
     7921 aatcttactc attttagccc ttacttgtgg tggttttggg gcctggttag ctggaatcac
     7981 ttttcaccac aagactcgaa aatggtactt taaaacagtt tggtttcttg ggatggtgac
     8041 cacactagta gccttatatt ttatttggag gtaatggatg gcagggtctt cgagggaaca
     8101 cgctgcttgg gttctagcgg actatggctt taaggtcgtg attgcaggat ctttcggtga
     8161 cattcattac aataatgaac tcaataatgg cctgttgcca atcgttcagc ctagggaggt
     8221 tagagaaaag ctagcccaac tcaaaccgac cgaccaagta actgtagact tggaacaaca
     8281 gaaaattatc tcaccagttg aagaattcac cttcgagata gatagcgagt ggaaacataa
     8341 actcctaaat agtttggatg atatcggtat taccttgcag tatgaagagt tgattgctgc
     8401 ttatgaaaaa caacgaccag cctactggca ggattagaaa aaatagaaaa ggaaatatag
     8461 taaactgaaa taagatgtaa acaaatgaat tagagcttaa catccatttc cagcaatttt
     8521 ttagaaacta cagtggacta ttctggattc aacacattat aaaattatga caaaacacat
     8581 tcacaagaag gctacgacat tttaaaaggt gagggcggat gtatcgtttg ccctactaaa
     8641 gttggttaca ttatcatgac cagtgacaag gcaggacttg agcgtaagtt cgcagccaaa
     8701 gagcgtaagc gtaacaaacc aggtgttgtt ctctgcggta gcatggatga actttgcgct
     8761 ttagcgcaac tcaacccaga aattgaagca ttctactaaa aacattggga tgaagatatt
     8821 cttcttggtt gtatccttcc ttggaaacca gaagcctttg aaaaactcaa agcatacggg
     8881 gatggccgtg aagaacttat gactgatgta cgtggtacta gctgttttgt tatcaagttt
     8941 ggaaaagcag gtgaacaatt ggctgccaag ctttgggaag aaggtaaaat ggtctacgcg
     9001 tcatctgctt caatgacaaa acgattgaaa ctcgctatga gcaaggtgta atggtgtcta
     9061 tggtcgataa ggacggcaaa ctcatcccag aacaaggagg agcacgttca acttcaccag
     9121 ctccagttgt gatccgtaaa gggcttgaca ttgatatagt aaaatgaagt aagagcagga
     9181 caaattaatc aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtgtacta
     9241 ttctagtttc aatccactat aaaatcatga tgcacctgtc agatactttt aactcatggg
     9301 actaccgtca ggttgagtat tattaggata gagaagaagt ctagtgttaa ggacattaca
     9361 gctcataata ctaggctttt ttatagaaat tcttttcttt ttctatagga tatggtattc
     9421 tatgtagaaa atatatgtta ataagtaatg ccaatattta aacatcatta gtaaaaggag
     9481 ttaga
//
LOCUS       DAVLYV010000076         9273 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLYV010000076 DAVLYV010000000
VERSION     DAVLYV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9273
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.076"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(283..588)
                     /locus_tag="VYO70_001719"
     CDS             complement(283..588)
                     /locus_tag="VYO70_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPAR17_0115 family bacteriocin-like peptide"
                     /protein_id="HEV1713914.1"
                     /translation="MELILPNNYVDLEQEEMMYLDGGEPMSISAIVALAITIGGASYS
                     TGYVLGERVYYSGYKNSDYQKNKWGIRGAMLAAFGAIGAVVMLGFENRYYALVTGRG"
     gene            complement(736..2088)
                     /locus_tag="VYO70_001720"
     CDS             complement(736..2088)
                     /locus_tag="VYO70_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEV1713915.1"
                     /translation="MKLYNKSELRYSRIFFDKRPPAFAFILIISTAIILSGALVGAAY
                     IPKNYIVKANGNSVITGTEFLSAIGSGKVVTLHKSEGDMVNAGDVIISLSSGQEGLQA
                     SSLNKQLEKLRAKEAIFQKFEQSLNEKYNHLSNSGEEQEYYGKVEYYLSQLNSENYNN
                     GTQYSKIQDEYTKLNKITAERNQLDADLQALQNELLQLQQQGDSPSLSDTTSADDKAK
                     LETKISEITTKIEALKTNITSKNSEIDSQQSNIKDMNRTYNNPTSQAYNIYAQLISEL
                     GTARSNNNKSITELEANLGVATGQDKAHSILAPNEGTLHYLVPLKQGMSIQQGQTIAE
                     VSGKEKGYYVEAFVLASDISRVSKGAKADVAITGVNSQKYGTLKGQVRQIDSGTISQE
                     TKEGNISLYKVMIELETLTLKHGSETVVLQKDMPVEVRIVYDKETYLDWILEMLSFKQ
                     "
     gene            complement(2085..2552)
                     /locus_tag="VYO70_001721"
     CDS             complement(2085..2552)
                     /locus_tag="VYO70_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1713916.1"
                     /translation="MAKLEVKDNKKLVLKSVICKKLHDTKVEDVDQEINKFHQHLQLL
                     KAQIFGPLIVKSCGTTIHDDGLITTDFEFYIQAHNAQQYSNIYDVQDSISVPYCLYVR
                     FEDSPEYLQYAYSKLDLYVYENDIQTDGIVYTVYVNSSPEKMVVDIFRPIVSL"
     gene            complement(2545..4764)
                     /locus_tag="VYO70_001722"
     CDS             complement(2545..4764)
                     /locus_tag="VYO70_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEV1713917.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     RNSKFYVADPASGIRKMSSDELGEIYQGITLFMVPNSDFERGKLKGKGLLDLFGRLIF
                     NQKGLISTVILASFVLSIIGILSSLFSKVIMDEVIPYALKNSLYMFLIVFGIVSFLQT
                     LLSAFRQHVLLFLSRKIDIPVLMGYYDHIIHLPYSFFGSRRVGDVLTRFQDAMTIKNV
                     FTSVSISLVMDIILSVISAVVLWTINQSLFLILVFMVIVNIILIYCFKKPYKKINHEQ
                     MEANGLLNSQLIESIRNIDTIKSQHDEEQRLNKIEEKFVHTLEIGYKEGVLQNIQSTI
                     SSMTSTMGGLLFMGVGALFIIDGKMTIGDLLVFQTLSQYFTEPIQNLVGLQLTFQEVQ
                     VAVSRLQELMEVDREDIALDYSIRDFTLCDDIEFKDVTFAYGSRPPVIKDFNLRIKQG
                     EKIAFVGESGAGKSTLVRLLLRFINPSEGKIRIGENDLSDLDYGKLRKKISYIPQTIE
                     LFTGTIIDNLKIGNPSVTYEDMVRVCRIVGIHDTIQRLQNRYGSFVEEGGQNFSGGEK
                     QRLAIARALLSKADLYIFDEATSNLDSFSEQIIQDLIFNKIIDKTTIVVAHRLSTILR
                     CDKICFLENGTIVEYGTHEELMAKNGKYARMVGLQSVQVNQQIQSQAVLDIEEVTYG"
     gene            5230..6093
                     /locus_tag="VYO70_001723"
     CDS             5230..6093
                     /locus_tag="VYO70_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1713918.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICRDDAKLIQKGFSLLELTEETSILSHLKK
                     EVEIYYQAKER"
     gene            complement(6382..7170)
                     /gene="argH"
                     /locus_tag="VYO70_001724"
                     /pseudo
     CDS             complement(6382..7170)
                     /gene="argH"
                     /locus_tag="VYO70_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(7230..8426)
                     /locus_tag="VYO70_001725"
     CDS             complement(7230..8426)
                     /locus_tag="VYO70_001725"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV1713919.1"
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDF
                     IHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKYPLVSALSRPLISKKLVE
                     IAHQTGATTIAHGCTGKGNDQVRFEVSIAALDLNLKVIAPVREWKWSREEEIYYAKEN
                     GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITTSPEQAPDMPEYIEI
                     EFSEGVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAV
                     TLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSSATQALIAYIKETQKVVN
                     GTAKVKLYKGSAQVVARKSPNSLYDENLATYTSADTVDQDAAVGFIKLWGLPTKVHSE
                     VQKSAK"
     gene            complement(8443..9249)
                     /locus_tag="VYO70_001726"
     CDS             complement(8443..9249)
                     /locus_tag="VYO70_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1713920.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
BASE COUNT         2887 a         1954 c         1382 g         3050 t
ORIGIN      
        1 atcgcaggct ttgtttagtt acctaaatta gacttccttc ctttatctta ttttaaatat
       61 ttaaaaccag ttactgcttg aagcttaaca gtttacttaa ttcaaataac agctaatcac
      121 tgattcccta attttatcaa atatactaat aacaacaatt gtgatacgat catcattata
      181 agaacaacat ttaacgaatc aaagtttttc cgagataata ataaagcaac agcaagcagt
      241 attattacta aagattgaat aactactatt actcttaaca ttttatcctc taccagtaac
      301 caaagcatag tatctatttt caaaaccgag cataactact gcacctattg ctccaaatgc
      361 agccagcatt gctcccctaa taccccactt atttttttga taatcagagt ttttatatcc
      421 tgaatagtag actctttctc ctaacacata tcctgtgcta tacgatgcgc caccaattgt
      481 gatagccaag gcaacaatgg ccgaaattga cattggttct ccgccatcaa gatacatcat
      541 ctcttcttgt tcaagatcaa cataattatt tggcaatatg agttccattt attttctcct
      601 tttttattaa gcatggttag gatagtttcc taacaagtaa tcgtcgactg tgtttctatc
      661 acttagctac ctagataaga tatcctttct ctaccttatt tatttttata ggttacctaa
      721 ggggtttaaa accaattatt gcttgaaact taacatttct aaaatccaat caagataggt
      781 ttctttatca tagacaatcc gcacttcaac tggcatatcc ttttggagga cgactgtctc
      841 acttccatgt tttagagtta aggtttctaa ttctatcatg actttataga ggctaatatt
      901 cccctctttc gtttcttggg aaattgttcc tgaatcaatc tgtctgactt gtccctttag
      961 tgttccatat ttttgactat ttacaccagt aatagcaaca tcagcttttg ctcctttcga
     1021 aacacgagaa atatcactcg caagtacaaa agcttctaca tagtaacctt tttctttccc
     1081 tgaaacttct gctatcgttt gcccctgctg aatagacatt ccttgtttca aaggtaccag
     1141 ataatgcaga gtaccttcat ttggcgctaa tatactatgt gctttatctt gacctgttgc
     1201 cactccaaga ttagcctcaa gctctgtaat acttttattg ttgtttgaac gagcagtacc
     1261 taactcacta attaattgag cataaatatt ataagcctga gaagttggat tattataggt
     1321 acggttcata tccttaatat tgctttgttg actatcaatc tcgctattct tagaagtaat
     1381 atttgttttt aatgcttcga tttttgttgt tatttctgat atcttagttt ctaacttagc
     1441 tttatcatca gctgatgtcg tatctgataa agaaggtgag tctccttgct gttgaagttg
     1501 aagcaattca ttttgtagag cttgcaagtc ggcatctaac tgatttcttt cagctgttat
     1561 tttattcaac ttcgtatatt catcctgaat ctttgaatac tgggtaccat tattataatt
     1621 ttccgagttt aactgagaca gatagtattc aactttccca taatattcct gttcttcacc
     1681 agaattagat aaatgattgt atttctcatt taatgattgt tcaaattttt gaaagatagc
     1741 ttcttttgca cgtaacttct ccaattgttt atttaaagag ctcgcttgta aaccttcttg
     1801 tccacttgat aacgaaataa tgacatctcc agcatttacc atatctcctt ctgacttatg
     1861 taaagtaact accttccctg aaccaattgc tgataggaac tctgtacctg ttataactga
     1921 atttccattc gcttttacaa tatagttttt gggtatataa gctgcgccaa ccaatgcacc
     1981 gcttaagata atagcagttg aaataatgag aataaacgca aaagctggtg gtctcttatc
     2041 aaagaaaata cgagaataac gtaattctga tttattatat aatttcatag gcttacaatt
     2101 ggtctaaaaa tatctactac cattttttca ggagaagaat taacataaac tgtatagaca
     2161 atcccatccg tttgaatatc attttcatag acatatagat ccaatttaga atacgcatac
     2221 tgtagatact ctggactgtc ttcaaaacga acatataaac aatatggaac agagatagaa
     2281 tcctgtacat cataaatgtt actgtactgt tgagcattat gagcttgaat ataaaactca
     2341 aaatcagtcg ttattaatcc atcatcatga atagtagtac cacaactttt tacaattaat
     2401 ggaccaaaaa tttgtgcttt taacaactgc aaatgttgat gaaatttatt aatttcctga
     2461 tctacatctt ctacttttgt atcatgtaac tttttacaga taactgactt tagtaccagt
     2521 tttttattat cttttacctc taacttagcc ataagtaacc tcctctatat ctaacacagc
     2581 ctgtgactga atttgttgat tcacttgaac gctctgcaaa ccaaccattc tagcatactt
     2641 tccatttttc gccattagtt cttcatgagt cccatattcc acaatagtcc cattttctaa
     2701 aaagcatatc ttatcacatc gaagaattgt agacagcctg tgggctacta caattgttgt
     2761 cttatctatt attttattaa agattaaatc ctgaataatc tgttcactaa atgaatctaa
     2821 gttagaggtt gcctcatcaa atatatacaa atcagcttta ctcagtagtg ctcttgcaat
     2881 agccaatcgt tgtttctctc cacccgagaa attttgaccg ccttcttcta caaaggagcc
     2941 ataacgattt tgaagacgtt gaatcgtatc atgaatacca acaatacgac aaactctcac
     3001 catatcctca tatgtaacag aaggattacc aatttttaga ttatcaataa ttgtccctgt
     3061 aaagagttct atggtctgag gaatgtagga aattttctta cgtagtttac catagtcaag
     3121 atctgataag tcattttctc ctatacgaat tttcccctca ctcggattta taaatcttaa
     3181 taacaaacga actagcgtac tttttcctgc tccgctttct ccaacaaaag ctattttttc
     3241 tccttgttta attcgcaaat taaagtcctt gataacagga gggcgtgaac cataggcaaa
     3301 tgttacatct ttaaattcaa tgtcatcaca taaggtgaag tctctaatac tataatccaa
     3361 ggcaatgtct tcacgatcta cttccatcaa ttcttgaagc cgactaaccg caacctgtac
     3421 ctcttgaaag gttaactgca agccaactaa attctgaata ggttctgtaa aatattgact
     3481 taatgtctga aaaaccaaga ggtccccaat agtcattttt ccatcaatga taaataaggc
     3541 gcccactccc ataaataata acccacccat ggttgaggtc attgaagata ttgtagattg
     3601 aatattctgc aaaacgccct ctttataacc aatttctaaa gtatgaacaa atttttcttc
     3661 aattttgttt aaacgttgtt cctcatcatg ctgagactta atggtatcaa tgttacgaat
     3721 agattcaatt aactgtgaat taagaagtcc atttgcttcc atttgttcat gattaatttt
     3781 tttataaggc tttttaaaac agtatataag aatgatgtta acaataacca taaaaactaa
     3841 tatcagaaat aaagattgat taatagtcca aaggacaacc gcactaataa ctgacaagat
     3901 gatatccatg actaatgaaa tagagactga cgtaaacaca ttctttatcg tcatcgcatc
     3961 ttgaaatctt gttaaaacat caccaactct acgacttcca aaaaaactat aaggaagatg
     4021 aataatatgg tcataatatc ccattaaaac cggtatatca atttttcgag atagaaacaa
     4081 aagaacgtgc tgacgaaatg cagatagaag agtctgtaag aacgacacta ttccaaatac
     4141 tatcaaaaac atatacagac tattttttaa agcatatgga atcacctcgt ccatgataac
     4201 ttttgaaaag agactcgata aaataccaat gatacttaaa acaaaggaag ctaatatgac
     4261 tgtactaatc aatccttttt gattaaaaat taaccttcca aataaatcaa gaagtccttt
     4321 tccctttagt ttccctcttt caaaatccga atttggaacc ataaaaagag taataccttg
     4381 ataaatttct cccagttcat cacttgacat cttgcgtatg ccactagcag ggtctgctac
     4441 gtaaaattta ctattccttt tgatactatg taataccaca aaatgatttt gacctaaatc
     4501 attcttaact tgaagaatcg caggaaatgt taattttgga gtcaaatctt ccagtgctac
     4561 tcgaacagct tttactgtaa aattcaactt attcagacct gaaacaatac cactgacagt
     4621 cgttccatac atgtctgtac caataatttc cctaatcttc atgattgata gttccttttt
     4681 gtaagataaa agaactgttg aaacaactgc cgcagcacaa tctgactggt catgttgcat
     4741 cgtacaagga tattttttta acatagaaat actcaattct ttagaattca tctaactttt
     4801 ctttgatgtc acccaaacct caatcactgc ccagtagttc ttgaattaat ttattttcaa
     4861 ccgtatcatt ctttgttctt gatatctttc caagcattgt agtacagcag atttttgccc
     4921 aaggactgag cctcctttaa caactcaaac tcccttacat tatatcggcc agccccatgg
     4981 aaagattata gccagactaa tcaatgatat aatacatact agaatacaaa atattttttt
     5041 attcatactt tttctcctat ctttattttc tacctacata gtacctctta ttaagctcat
     5101 tttaaaatta cgttaacttt tttcacaaat ttatttcgac tttctttttt actttttaca
     5161 ttgctatgat acaatatttt taacaaaatt ttattgtttg ttaacttttt tcacgaaaag
     5221 gagaaaatca tgcgttatga tttcggaaag gtctataaag aaatacgtga gtcaaagggg
     5281 ctgacccaag aagaggtctg tgggggtgtt ctctcaagaa ccagcttatc aaaaatcgaa
     5341 agtggcaaga caaccccaaa atatgaaaat atggaatttc ttctccgtca aatcaatatg
     5401 agttttgaag agtttgaata tatctgtcaa ctttatcaac caagtcaacg cacagagatt
     5461 atgcaaacct atctcaatat gcgttccatt atcgggacca gcgatcttgt caatttattt
     5521 caaaaatgcc aagactacct caagacccac cacgatctcc ctatagaaga aatcagagat
     5581 atgcttgaag ttgttattta tctccgtcaa cacggggcag gggaactatc aaaacacgca
     5641 gaacaggttg tcaaaaaact ttggaaaaaa attgaaaaac aagatacatg gtatgagagt
     5701 gatctaaaaa tcctcaatac cattcttttc agttttccca ttgaatacct ccatctcatc
     5761 actggaaaaa tattgcaacg cctagaagtc tataaaaact atcaacattt atatgacttg
     5821 cgaatgacaa ttctattaaa cctttccaca ctctatctat acaatcaaga taaaaacatg
     5881 tgtaagcaaa tctgctacac tttactggag gacgccaaga ataagaaaag ctacgatagg
     5941 ctggctatct gctatgtccg tattgggatt tgtagggatg atgcgaaact tatccaaaaa
     6001 gggttctccc ttctggagct aaccgaggaa acttctatac tgtctcatct caaaaaagaa
     6061 gtagagatct attaccaagc gaaggaaaga taaaaaagcc gagggatttc ctcgactctt
     6121 tacatattat ttgttcgtta gttcttaata ccagccattg ttaagccaga agtttttagc
     6181 tctgccaagc cacatagtgt gtttactact atgtgtccat gtcaccatga atcgccgaac
     6241 gatttaacga gaaagtatga cttttacgtt tatcccaact caattatgac atttttttca
     6301 aaagtcaata tatctcactt tttcaacgac aagaaagagg ctgccaatcg accaacctct
     6361 tattctgaac ccatcactcc atcacttttt agcttcattc gctttcttag cgactgcaat
     6421 ctggtattcg acttggtcaa accctgtgcc tcccaaggaa tttcgtcttt gaacagcagt
     6481 tttaggttgc aagtagacat agatatcctc ggtaatgaga gaatgatagg tttgtaattc
     6541 ctccaaagtc caatcttgaa gatttttagc agacttgata gagtctagca ctaatcttcc
     6601 cacaacctca tgagcttctc taaatggcaa gcctttccct gccaaataat ctgccaactc
     6661 agttgcattt gaaaagtcct tctctgttga ttcttgcatt ttttccttgt ttacctgcaa
     6721 actagataac atacctgcca acacatcgag ggaatttaaa atcgtttcga ccgtatcaaa
     6781 cattccttcc ttatcttctt gcaaatcctt attataagct aaaggcaagg acttcatgac
     6841 tgtcaacagt ccaaacaaat gcccataaac tctgccagtc tttcctcgaa tcaactcagc
     6901 catatctgga tttttctttt ggggcataat agaggaacct gttgtaaacg tatctgacaa
     6961 ggtaatgaat tggtactcaa aactacacca attgatcatt tcttcgcaaa aacgactcat
     7021 atgcatcatg agtatgctgg catttgatag aaattctaag ataaaatcgc ggtcactaac
     7081 agcatccaaa gaatttgtat aaggttgctt aaactccagc aaatcgctag acaattggcg
     7141 atcaatgggg aaagtcgttc ctgccaaagc gaccacccca taattttgta ttcttagcca
     7201 tatcacatcc acctctctat ccaatcgtcc tatttggcgc ttttttgaac ctcagaatga
     7261 accttggttg gaagtcccca taacttaata aagccaacag ccgcatcttg gtcaacggta
     7321 tctgcactag tatacgtcgc caaattttca tcgtaaagag aatttggtga tttccgagcc
     7381 acgacctgag cgctcccctt atacagtttg acttttgcag tcccattgac aactttttga
     7441 gtctctttaa tataagcaat caaggcttgt gtagccgaac taaaccacaa agcattataa
     7501 atgagatttg ataattcatt ttctatgatt ggtttaaaat gagccacttc tctcacaagc
     7561 gtcaagtcct caatttcctt atgagctgtc agcagggtca ctgcacctgg acactcatag
     7621 atttctcttg atttaatccc aaccaagcga ttttccacgt ggtcaatccg tccaacccca
     7681 tgttttccag ctatttcatt gagtttttga atcaaatctg ccaattttaa cacttctcca
     7741 ttgagggaaa ctggaacacc ttcactaaac tcaatctcaa tgtattctgg catatctgga
     7801 gcttgctctg gagaagttgt gatgccaaag gcttcttctg gagcctgatt ccatggattt
     7861 tccaaaatac cacactcatt ggcacgtccc caaagatttt ggtcgacaga gtagggattg
     7921 tcaaggtcag caggaacagg caccccattt tccttggcat aataaatttc ttcctcccga
     7981 gaccatttcc attcacgaac tggagcaatc acttttaaat tgagatccaa ggctgcaatc
     8041 gatacttcaa aacgtacttg gtcattcccc ttacctgtac aaccatgagc aattgtagtc
     8101 gctcctgtct gatgcgctat ttcaaccaat tttttagaaa taagagggcg gctcaaggca
     8161 gataccaagg ggtacttttg ttcatagtag gcatgtgact ggagagctac tagcacataa
     8221 tctgtagcaa attcgtcctt aacatcaatg acataagatt cgactgcccc aaccttaaga
     8281 gccttatcat ggatgaaatc taagtctttc ccttcaccca catccataca aactgcaaca
     8341 acatcatagt ctttcttcag ccaggtaata gcaactgatg tgtctaaacc gccagaatag
     8401 gccaaaatta ctttttcttt actcatgttc ttttccttct ttctattttt caatggacgc
     8461 tttaaaggca tcctcaatga gtttgtctaa ttccccagac tccttcaact tttgaatggt
     8521 tttatcgact gcctctttca attccttgct atcttttttc atggctaccg cataggaatc
     8581 atcttgctct ttttcaaaat tgaggtctgc gattgctaaa tcaggattat tttccacaaa
     8641 tcccttggca acgggttctt caaaaataac ggcatccact tgccctgatt ttaaatctgt
     8701 gattaaattc ccatttttag gcagagatac gagggaagaa ttttgtagca aatctttcgc
     8761 catcgtctct tgaatcgaac ctttctgcgc tccgaccttt ttctgcgcca aatcgttgac
     8821 agactgataa gtggctaaat cagatttttt gacaatgagt ttattttttg cagtatagta
     8881 gggagttgaa aaatcaaaca ccttgctccg ttcatctgtc ttagaaacac ctgatatggc
     8941 aaggtcagct tttcctgatt gaacgctagc cagtacattg tcaaaactca taggagatag
     9001 ttccaattcg acacctagtt ctgttgcgat agccttggct aattcgatat ctgaacccac
     9061 aatctgattt ttcccatcaa ccactttttg atattcaaat ggagcaaaat ctggattgag
     9121 ggctacaacc aattttcctt tggacttgat ggcttcaatt ccagccgatt gattgttact
     9181 acaagcaaat agtaggggga gcatcactaa accaaacatc gtcatcaaca ccttcttcat
     9241 cttattcata acaaagcctc ctttatgttt att
//
LOCUS       DAVLYV010000077         9246 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLYV010000077 DAVLYV010000000
VERSION     DAVLYV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.077"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            46..1896
                     /locus_tag="VYO70_001727"
     CDS             46..1896
                     /locus_tag="VYO70_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1713921.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQEIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(2078..2176)
                     /locus_tag="VYO70_001728"
                     /pseudo
     CDS             complement(2078..2176)
                     /locus_tag="VYO70_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(2202..2834)
                     /locus_tag="VYO70_001729"
     CDS             complement(2202..2834)
                     /locus_tag="VYO70_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1713922.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(2856..3728)
                     /gene="sdaAA"
                     /locus_tag="VYO70_001730"
     CDS             complement(2856..3728)
                     /gene="sdaAA"
                     /locus_tag="VYO70_001730"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1713923.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(3737..4408)
                     /gene="sdaAB"
                     /locus_tag="VYO70_001731"
     CDS             complement(3737..4408)
                     /gene="sdaAB"
                     /locus_tag="VYO70_001731"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1713924.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            4650..5222
                     /locus_tag="VYO70_001732"
     CDS             4650..5222
                     /locus_tag="VYO70_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1713925.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(5275..5595)
                     /locus_tag="VYO70_001733"
     CDS             complement(5275..5595)
                     /locus_tag="VYO70_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1713926.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTPYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSMLSHLKKEVETHYQ
                     PKKL"
     gene            6020..6307
                     /locus_tag="VYO70_001734"
     CDS             6020..6307
                     /locus_tag="VYO70_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1713927.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            6359..8467
                     /locus_tag="VYO70_001735"
     CDS             6359..8467
                     /locus_tag="VYO70_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1713928.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            8464..9105
                     /locus_tag="VYO70_001736"
     CDS             8464..9105
                     /locus_tag="VYO70_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1713929.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
BASE COUNT         2701 a         1683 c         2019 g         2843 t
ORIGIN      
        1 ataataacaa tatagtaaaa ctcttttaag gaggagtaga tttctatgaa taaaaaacta
       61 acagattatg tgattgatct ggtggaaatt ttaaataaac aacaaaagca ggttttctgg
      121 ggaatatttg atattttcag tatggtggtt tccatcattg tatcttatat tttattttat
      181 gggctgatta atccagcacc tgttgactac attatctata cgagtttggc cttcctgttc
      241 tatcaattga tgattggttt ttgggggttg aacgcgagca ttagtcgtta cagcaagatt
      301 acggatttca tgaaaatctt ttttggtgtg actgctagca gtgtcttgtc atatagtatc
      361 tgttatgcct tcttgccact cttctccatc cgtttcatca ttctctttat cttgttgagt
      421 accttcttga ttttattgcc acggattact tggcagttaa tctactccag acgcaaaaaa
      481 ggtagtggtg atggagaaca ccgtcggacc ttcttgattg gtgccggtga tggtggggct
      541 ctttttatgg atagttacca acatccaacc agtgaattag aactggtcgg tattttggat
      601 aaggattcta agaaaaaggg tcaaaaactt ggtggtattc ctgttttggg ctcttatgac
      661 aatctgcctg aattagccaa acgccatcaa atcgagcgtg tcatcgttgc gattccgtcg
      721 ctggatccgt cagaatatga gcgtatcttg cagatgtgta ataagctggg tgtcaaatgt
      781 tacaagatgc ctaaggttga aactgttgtt cagggccttc accaagcagg tactggcttc
      841 caaaaaattg atattacgga ccttttgggt cgtcaggaaa tccgtcttga cgaatcgcgt
      901 ctgggtgcag aactgacagg taagaccatc ttagtcacag gagctggagg ttcaatcggt
      961 tctgaaatct gtcgtcaagt tagtcgcttc aatcctgaac gcattgtctt gctcggtcat
     1021 ggggaaaact caatctacct tgtttatcat gaattgattc gtaagttcca agagattgat
     1081 tatgtacctg tgattgcgga cattcaagac tatgatcgtt tgttgcaagt ctttgagcag
     1141 tacaaacctg ctattgttta tcatgcggca gcccacaagc atgttcctat gatggagcgc
     1201 aatccaaaag aagccttcaa aaacaatatc cgtggaactt acaatgttgc taaggctgtt
     1261 gatgaagcta aagtgtctaa gatggttatg atttcgacag ataaggcagt caatccacca
     1321 aatgttatgg gagcaaccaa gcgcgtggcg gagttgattg ttactggctt taaccaacgt
     1381 agccaatcaa cctactgtgc agttcgtttt gggaatgttc ttggtagccg tggtagtgtc
     1441 attccagtct ttgaacgtca gattgctgaa ggtgggcctg taacggtgac agacttccgc
     1501 atgacccgtt actttatgac cattccagaa gctagccgtc tggttatcca tgctggtgct
     1561 tatgccaaag atggggaagt ctttatcctt gatatgggca aaccagtcaa gatttatgac
     1621 ttggccaaga agatggtgct tctaagtggc cacactgaaa gtgaaattcc aatcgttgaa
     1681 gttggaatcc gcccaggtga aaaactctac gaagaactct tggtatcaac cgaactcgtt
     1741 gataatcaag ttatggataa gattttcgtt ggtaaggtta atgtcatgcc tttagaatcc
     1801 atcaatcaaa agattggaga gttccgcact ctcagtggag atgagttgaa gcaagctatt
     1861 atcgcctttg ctaatcaaac aacccacatt gaataaaaaa gaaaaacgca tagtatcaag
     1921 ttacacaacc ttggtaatat gcgttttatt atgtagagac ttatactctt cgaaaatctc
     1981 ttcaaaccac gtcaacgtcg ccttgccgta tgtatatgtt actgacttcg tcagttctat
     2041 ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tccatctaca accttaaaac
     2101 agtgttttga gcagcccgca gctagtttcc tagtttgctc tttgattttc attgagtatt
     2161 acttcatttt cttctgaaat agagttgtcg cccagtctat gctattgaaa atacgccaaa
     2221 acttctaagg gtttgtgagc gatataatca ggttgatagt ttagtagatc tgcttgctct
     2281 ccaaatcccc aagtgacggc caatttctga atacctgttt ctcgagctcc cagcatatca
     2341 aacttggtat ctccgatgat gatggcttgt tctggtgcta gttgatgtgt ctgtaaggct
     2401 tggtgaatga catctgcctt atggggtgct tcagggctag aaccataaat accatcaaag
     2461 aaatgatgga tttccaagtt tttggccatg tcttgagcag tagatgtatt ctttgtcgtg
     2521 gtgatgtaga gtggataact gctcgataac tcctcaagca agtctataat ctgaggaaag
     2581 agttgagctt catagatgcc ttttgcctta tagtaagaac gatatatctg catggcttca
     2641 gaaatttggt ctttggacag gcaggtcgca aaactacttt cgagaggtgg tcccataaaa
     2701 ccacgaatag ttttggcatc agggctaggc acccccagct ctttaaaggt ataggtaaag
     2761 gcattgtgaa tcccgataga actatcaacg agggttccat ccaaatcgaa aaaaatcgct
     2821 gtgatagagg tcatggtttc tcctatttga taagcttatt ctccgaaaat ctctttttgg
     2881 aggcgacgac cagtaggggt ggtagcgagt ccaccttcag ctgtttcacg aaaggcagtt
     2941 ggcatgcttg ctcctacttg gtacatggca tcgatcactt catccacagg gattttagat
     3001 tcgatacctg ccaaggccat gtctgctgcg atgaaagcaa agctagctcc catggcatta
     3061 cgtttgacac agggaacttc gaccaaacct gcaacagggt cacagatgag gcctagcata
     3121 tttttaatga caaaggcaat agcttgactg gcctgataag gtgttccacc tgcagccaga
     3181 gtcaaggcgg cagcactcat agcagaggct gaaccaactt cagcttgaca tccaccctca
     3241 gcacctgaga tggaggcatt gtttgcgatg actagtccaa aggcaccagc agcaaagagg
     3301 aaatccaatt gttgctcgtg gctgaggtct aatttttcaa tagcagcagt gagaacggaa
     3361 gggagacaac cggcacttcc tgcggtagga gtagcgcaga ccaagcccat tttagcattg
     3421 tgttcattga ctgcgatagc atttcgggca gcagagagaa tcgtgtaatc tgataaagtt
     3481 ttttcgtttt tgatgtagtg atccaatttg gcagcatctc cacctgtcag gccactacga
     3541 gatttatttt cattgaggcc aagttggaca gaggctttca taacttccag attgcgttcc
     3601 atgagaagga agacttcttc acgttcgcga ccggtcaatt caaactctgt tgtaatcatg
     3661 agttctgcga catttccttg aaagtccaga tctgcttgct cgaccaattc tttgatagaa
     3721 taaaacatgc ttcctcctat ttaaagaaat tgacattgtg gagatgaggg atttttcgaa
     3781 tttcttcgat agcctcatca cagttgcgac tgtcaacttc gataatcata atggcttttt
     3841 caccagcttt ttcacgagtg acattcatct gggcgatatt gataccatag cgggaaagcg
     3901 cctctgtaac aagggcaatc atacctggaa tatcttgatg aacgatgatg atagtcggtg
     3961 tattcatatt gagagagacg gcaaaaccat tgagttcggt tacctgaata tttcctccac
     4021 cgatagaaat accagtcacg ctgatggtct tgtgggcatt tttaacagta attttagtgg
     4081 tgttagggtg aggggcattg ctgtctttct gaatggtcca gacaatcttg ataccacgct
     4141 tgtgggcaat ttccagacta tttggaattt caggatcatc tgtatccatt cctaaaatac
     4201 ctgcaacaag ggctaggtct gttccgtgac cacgataggt cttggcaaat gagttaaaaa
     4261 gttggaattc aacttctgtc ggagtatcat caaaaatgga agagacaatc ttcccaatac
     4321 gaacagcacc agcggtatgg ctactagatg ggccaatcat aactggtccg atgatatcaa
     4381 agacagattg aaaacgaagt gatttcatca gtttcccctt ataaaaattc ttatctctat
     4441 tatatcaaag aatgaggggc ttggctttaa ttgtggatga aaacctttct aatacctcaa
     4501 atagcataaa aatagtatct tttatgacaa aaaacacctt atttagggaa ataaaaaata
     4561 attttgtaat atttctacat aaaagtgtca agaaacggta atatttaaag ggtatgatag
     4621 aactatagaa agaaggagaa ttttcgaata tgaaatcaat aactaaaaag attaaagcaa
     4681 ctcttgcagg agtagctgcc ttgtttgcag tatttgctcc atcatttgta tctgctcaag
     4741 aatcatcaac ttacactgtt aaagaaggtg atacactttc agaaatcgct gaaactcaca
     4801 acacaacagt tgaaaaattg gcagaaaaca accacattga taacattcat ttgatttatg
     4861 ttgatcaaga gttggttatc gatggccctg tagcgcctgt tgcaacacca gcgccagcta
     4921 cttatgcggc accagccgct caagatgaaa ctgtttcagc tccagtagta agtgaaacag
     4981 ttgtttcaac tgtaagcgga tctgaagcag aagccaaaga atggatcgct caaaaagaat
     5041 caggtggtag ctatacagct acaaatggac gttatatcgg acgttaccaa ttaacagatt
     5101 catacctgaa cggtgactac tcagctgaaa accaagaacg tgtagcagat gcctacgttg
     5161 caggacgtta cggttcatgg actgctgcta aaaacttctg gcttaacaat ggctggtatt
     5221 aagaactaac gaacaaataa tatgtaaaag gtcgaggagc tccctcgact ttttttataa
     5281 tttctttggt tgataatggg tctctacttc ttttttgaga tgagacagca tagaagtttc
     5341 ctcggtcagc tccagaagtg agaaaccttt ttggataagt ttagcattat ctgtacaaat
     5401 cccaatacgg acatagcaga tagcaagcct atcgtagctt ttcttattct tggcgtcctc
     5461 cagtaaagtg tagcagattt gcttacacat gtttttatct tgattgtata gatagggtgt
     5521 ggaaaggttt agcaggattg ctattcgcaa gtcatataaa tgttgatagt ttttatagac
     5581 ttctaagcgt tgcaagattt tctcggatta ttgacaaata aacggacatt ctgatagacg
     5641 attgccctag cggcttagtg taaactttac aaatttggct tgtaattttt ttataaaatt
     5701 cacttcagtt acttgacagt tattatattt tcgcttatta tatagataaa aggatgggat
     5761 aggattttaa aaatttgaaa gcgctatcca tattgtttat tggactgatt cttgagtaat
     5821 agttagagtg tgcttaatac tactctggtt gttcaaggac ctgatacaga agcgcaccat
     5881 tctcccggtt aagagtaagt tcgttgttaa aaaatgaaat gagatagaag gaaggtgagt
     5941 ccaatggact acgatacttg ttgataaatt actatagctg tttttacgga acagctctcg
     6001 ataataggag aaataaatca tgagaaaatt agttaaaatt ggtgttgcat ctttaatggt
     6061 atgtggtata cttgctacta caaatgcttt agcagtatgg gttgagggtg gtcaatggaa
     6121 ctatggagta ggttggacag gaacttttgg atattctgat tacttacatt ctactcgata
     6181 tcatacagca actgttagac atgggggtag aacctctaag gattatgcaa aacctgaggc
     6241 atgggctaga gcttccctca ccaagattcc gccaacagga atggaatatt tctatggatt
     6301 tgagtaaaat tcttccctct cctatttgga gagggaaaac tgtagtggag gcacttttat
     6361 gaaaaaaatc agtaatttct gtatgttact cctgcttctg tgtaccactt tttttgtttt
     6421 taatgtaaac tatacacgag aagtggttcg gattcaagaa atgggaaaga ctgtagattc
     6481 tttggatttg tatttgaaag atattaacga acctgcagcg tctgttcttc gattttttga
     6541 ggatgtatca aaggagtata aagtctccat catcaaaaca gacagtggtg atgaggtggt
     6601 caagtctggt gtttttgata aagatacctt cccctaccaa gagtttggga tttcttctct
     6661 tgattttacc acagatggtg aaggagtcta tagtaataaa gaaatttcca ataaacttgg
     6721 tacgattccg acctttctaa aagccaaacc tattcagctt atgacttttc aaacctatat
     6781 caaggataca tctcgtagtt taaatggtcg ctatacgata acttctacac aagagatgga
     6841 caaggatagg attgtacaga aatggagcga ttttttcaag atagaccagg ctaccttgct
     6901 agagccgacc tacaaaagtg cagtggaagt cataaatcga gatttgcttt tatctgccat
     6961 tgtttttgtc ttggctattt tgcttcttgt gttagtgaca gtgtatcaac cgatgatgga
     7021 gatgaaaaga gttggggtac aaaaattact tggttttcaa gatagggctg ttttagctga
     7081 tgttgtaaaa ggcaaccttt acctcctcct aggtggggct cttgtgatca atctaggcgt
     7141 gtttttcttg cttgattata agccaaaaga tttgtttcct atgctgtggt tgtctcattt
     7201 tttgctgttg cagctttatc tctttatcag ttggttgact tacctcttaa tccaaaaaat
     7261 gacaatcagc tctctgctga aaggtttttc atctttcaaa tttggtctta tcttcaatta
     7321 tgtgatgaaa atagggacaa ctattttact gacggcctta ctgattgggg tgggcagaag
     7381 tttagaacaa gaaaacaaag aacttgctta tcagcaacag tgggtaagtc aaggtaatta
     7441 cctgacctta gaaaccttca aactcaatga taatctgtgg caagaagagc tagcagggtc
     7501 agggaaatct acagattatt tctatcgatt ttatcaggat ttggtagaaa aaacgcaggc
     7561 gggctatgtg cagagtagca gtcttcctgt aaaaaatttt gtccaatcag aacagattca
     7621 gcaatatcag ttaacagata cggtggatgt ttactatgcc aatcgcaatt ttctaaagag
     7681 caagggattc aagctaccag ataccggtat taaaaaagtt attttgatgc cagcaagtac
     7741 gaaaggtgaa gaagataaaa atcagctctt ggggaagtta attgcctttc attcgatgaa
     7801 gtatgaagag cagcaaaaac gaacgataga ggagatggat gtcgagattg cctattatga
     7861 aggagattgg tcatttttcc catatagtga taagcgaaag gaaaatctct ccaatccaat
     7921 tattagcttg gtcaatgatt ctgatatgat gtgggatgag aaagcctccc tgtcaacaac
     7981 tggcttaaat aatccgatta aaattgaaaa tacggttcaa catcaaaaag agattacaga
     8041 gttagttgag aaattgtcag atggaaatta tttaaaattt tcatctattc aagccattca
     8101 acaagagaaa gtggattctt atcgagatgc tgttcggaat tttaacctac tctttgcttt
     8161 gtttggtctc cttagcatga tgatttccta cttcttacta gtaacaactt tcttattgaa
     8221 gcgcagggat atcattacca agaagtttat ggggtggaaa ctggtcgatc gctaccgtcc
     8281 tctcctcgtt ctgctcttgc tgggctatag tttccctctt ctagtcttga ttttctttgc
     8341 ccatgcgttc ttaccacttc tactgtttgc aggttttaca tgtctggata tactatttgt
     8401 gctaggctta gcttctagga tggagaaaag aagtctagta gagttattga aagggggcat
     8461 cttatgattg agttgaaaaa tattaccaaa accattgggg gaaaagtgat tttggataac
     8521 ttatctctca ggattgatca gggggatttg gtagctattg ttggtaagag tggtagtggg
     8581 aagtcgacct tgttaaattt attgggtttg atagatggtg attatagcgg acggtatgag
     8641 atttttggtc agacaaatct agcggttaat tctgctaagt cgcaaacaat aatccgtgaa
     8701 catatctctt atctgtttca aaattttgcc ctgattgatg atgaaacggt cgagtacaat
     8761 ctcatgctgg cgctgaaata tgtgaaattg cctaagaaag acaagctcaa aaaggtggaa
     8821 gagattttag agagagtagg tttgtcagct actttgcatc aaagggtctc cgagttgtct
     8881 gggggcgaac aacaacgaat tgcagttgct agagccatct taaaacccag ccagctgatt
     8941 ttagccgatg aacctacagg ttcgctggat cctgaaaata gagatttggt cttgaagttt
     9001 ctcttagaga tgaatcgaga ggggaaaaca gtcattatcg tgacccacga tgcttatgta
     9061 gcccaacaat gtcatcgaat cattgaatta ggcgagggaa aatgagttcg ttcagcttca
     9121 tttgaccgac tgaatactca tatttttcag agagaaatag cataaatagg cctaggaatg
     9181 atatttttat gtcattttcc tgggtttttc tgttttaaat tgaaaaaaaa tacaatttag
     9241 gcttga
//
LOCUS       DAVLYV010000078         8979 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLYV010000078 DAVLYV010000000
VERSION     DAVLYV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.078"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            186..1973
                     /locus_tag="VYO70_001737"
     CDS             186..1973
                     /locus_tag="VYO70_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1713930.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFDGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            1970..2446
                     /locus_tag="VYO70_001738"
     CDS             1970..2446
                     /locus_tag="VYO70_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1713931.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            2474..3379
                     /locus_tag="VYO70_001739"
     CDS             2474..3379
                     /locus_tag="VYO70_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1713932.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            3366..4181
                     /locus_tag="VYO70_001740"
     CDS             3366..4181
                     /locus_tag="VYO70_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1713933.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            4188..4592
                     /locus_tag="VYO70_001741"
     CDS             4188..4592
                     /locus_tag="VYO70_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1713934.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            4845..6011
                     /locus_tag="VYO70_001742"
     CDS             4845..6011
                     /locus_tag="VYO70_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1713935.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            6127..7164
                     /locus_tag="VYO70_001743"
     CDS             6127..7164
                     /locus_tag="VYO70_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1713936.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            7372..8226
                     /locus_tag="VYO70_001744"
     CDS             7372..8226
                     /locus_tag="VYO70_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1713937.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWNIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            8376..8633
                     /locus_tag="VYO70_001745"
     CDS             8376..8633
                     /locus_tag="VYO70_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HEV1713938.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDCDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
BASE COUNT         2559 a         1691 c         2032 g         2697 t
ORIGIN      
        1 tatattccga atttaaagag cgtttgaaat gggtctaaaa atgtttgaaa ctaattgaat
       61 gtgtgtataa tagtaaaaat gcagtaaata tcccgacaat ttctattgac aattcaaaca
      121 gattggttta taattaatat aacaacaaat gaaagcgcaa actttcgcgg tcggaaggta
      181 gttttatgac acgatttgag atacgagatg atttctatct cgatggaaaa tcatttaaga
      241 ttttatctgg tgccattcat tattttaggg ttcctccaga ggattggtat cattcgctct
      301 ataacttgaa ggctcttggt tttaatacgg tagagactta tgttgcttgg aatttacacg
      361 agcctcgtga aggtgagttt cattttgacg gtgatctgga tttagagaaa tttctccaaa
      421 tagcgcagga tttgggtctc tacgcaattg tgcgtccgtc tccatttatc tgtgcggaat
      481 gggaattcgg tggcttacca gcttggctct tgaccaagaa catgcgaatt cgctcatccg
      541 acccagcata tatcgaggca gttggtcgct actatgatca gttattgcca agactggttc
      601 ctcgtttgtt ggacaatggt ggcaatattc tcatgatgca ggttgaaaat gagtatggtt
      661 cttacggaga agataaggct tacctgagag cgattcgaca gctaatggaa gagtgtggcg
      721 taacctgtcc cctctttaca tcagatggtc catggcgagc tactctgaaa gctggaacct
      781 taattgaaga ggacctcttt gtaacaggaa actttggttc taaggcacct tacaactttt
      841 cgcagatgca ggaattcttt gatgaacatg gtaagaaatg gccactcatg tgtatggagt
      901 tctgggatgg ttggtttaat cgttggaaag aaccgattat cacacgggat cctaaggaat
      961 tggcagatgc agttcgagaa gttttggaac aaggctccat caatctttac atgttccacg
     1021 gtggtacaaa ctttggtttc atgaatggtt gctcggctcg aggaactttg gacctgccac
     1081 aagttacgtc ttatgattac gatgcccttc tggatgaaga aggaaatcca actgctaaat
     1141 atcttgcagt caagaagatg atggcaacac atttttcaga gtatccgcag ttggaaccac
     1201 tctacaaaga gagtatggag ttggatgcta ttccactagt tgaaaaagtt tctttgtttg
     1261 aaaccttaga tagcttgtca agtcctgtag aaagtctcta tcctcaaaag atggaggagc
     1321 tgggacaaag ttatggctac ctactttatc gaacagaaac aaactgggat gcagaagaag
     1381 aaagacttcg tatcattgat ggtcgagata gggcccagct gtatgtcgat ggtcagtggg
     1441 ttaaaactca atatcagaca gagattgggg aagatatttt ttatcaaggt aaaaagaaag
     1501 ggctatctag gttagatatc ttgatagaaa atatggggcg tgtcaactat gggcataagt
     1561 tcttagcgga tacgcaacgt aagggaattc ggacaggggt ctgtaaggat ctgcatttct
     1621 tactaaactg gaaacactat ccactcccac tagacaatcc tgagaaaatt gatttttcaa
     1681 aaggatggac tcaaggacaa ccagcctttt acgcttatga ctttacagtc gaagagccaa
     1741 aagatactta cctagacttg tctgagtttg gtaagggggt tgcctttgtc aatgggcaga
     1801 atctaggacg tttttggaac gttggcccaa ctctctcact ttatatccct catagctatc
     1861 tcaaggaagg tgccaaccgc atcattatct ttgaaacaga aggtcaatat aaagaagaga
     1921 ttcatttaac tcgtaaacct acactaaaac atataaaggg ggaaaactta tgacaattgt
     1981 aggatgccgt attgatggac gtttgatcca cggacaagta gccaatcttt gggctggaaa
     2041 actaaatgtt tcacgcatta tggttgtaga cgacgaagtt gtcaacaacg atattgaaaa
     2101 gagtggtttg aaacttgcga caccaccagg tgtgaaattg agtattttgc cagttgagaa
     2161 agctgcagcc aatattcttg ctggcaaata cgatagccaa cgtctcttta tcgtggctcg
     2221 taaaccagac cgcttccttg gtttggtaga agcaggtgta ccacttgaaa cccttaatgt
     2281 tgggaatatg tctcaaacac cagaaactcg ttctattaca cgttctatca acgtagtaga
     2341 caaggatgtg gaagacttcc acaaactggc agaaaaaggt gttaaactta ctgctcagat
     2401 ggttccaaat gatccaattt cagacttttt gagcttatta aaataggaaa aaatttttta
     2461 ggaggtcatt gttatgatac aatggtggca aattttactt ctcactttgt actcagctta
     2521 tcaaatctgt gatgagttga cgatcgtttc atctgcaggt tcccctgtat ttgctggttt
     2581 cattactggt ttaatcatgg gagatgtgac tactggttta cttatcggtg gtaacttgca
     2641 actgttcgtt cttggggttg gtaccttcgg tggtgcttct cgtatcgacg caacttctgg
     2701 tgcggttctt gcgacagcct tctctgtttc acaaggaatt gatgcaccgc ttgcaattac
     2761 tacaatcgct gtaccagtag cagctctctt gacttacttc gacgttcttg gtcgtatgac
     2821 tactaccttc ttcgctcacc gtgtggatgc tgcaatcgaa cgctttgact ataaaggtat
     2881 tgaacgcaac tacttgcttg gtgcgattcc gtgggctcta tctcgtgccc ttccagtctt
     2941 ctttgccctt gcttttggtg gtgcctttgt acaatcagta gtagacttcg ttgaagccta
     3001 caaatgggtt gcagatggct tgacactcgc aggacgtatg cttccaggtc ttggatttgc
     3061 aatcttgctt cgttaccttc cagttaaacg taaccttcac taccttgcta tgggatttgg
     3121 tttgacagct atgttgactg ttctttactc atatgtaaca ggtcttggtg gcgctgttgc
     3181 tggtatcgta ggtactcttc ctgctgaagt tgctgaaaaa attggtttcg tgaacaactt
     3241 caaaggtttg tctatgattg gtatttctat cgtaggtatt ttccttgcag tgcttcactt
     3301 caaaaatagc caaaaagtag ctgtagcagc accttctaca ccatcagaaa gtggggaaat
     3361 cgaagatgac gaattctaat tacaaactta caaaagaaga ttttaatcaa atcaacaaac
     3421 gtagcttgtt tactttccaa ttaggttgga actacgaacg tatgcaagct tctggttacc
     3481 tttacatgat cttgcctcag ttgcgtaaaa tgtatggtga tggaactcct gaattgaaag
     3541 aaatgatgaa agttcatact caattcttca atacttcacc attcttccat accattatcg
     3601 ctggttttga ccttgccatg gaagaaaaag atggtgtagg ttcaaaagac gctgttaacg
     3661 gtatcaagac aggtttgatg ggaccattcg ctcctcttgg ggatacaatc tttggttcac
     3721 ttgtacctgc tatcatgggg tcagtcgcag caactatggc tatcgctggc caaccttggg
     3781 ggatcttcct ttggattgca gttgcagtag cgtatgacat cttccgttgg aaacagttgg
     3841 aatttgctta caaagaaggg gttaacctta tcaacaacat gcaaagtacc ttgacagctt
     3901 tgattgacgc tgcatctgta cttggtgtct tcatgatggg tgctcttgta gcaacagtga
     3961 ttaactttga aatttcttac aagttgccaa tcggtgaaaa gatgattgat ttccaagaca
     4021 tcttgaacca aatcttccca cgtttgcttc cagcaatctt tactgccttt atcttctggt
     4081 tgcttggtaa aaaaggtatg aactctacta aagctatcgg tattattatc gtacttgctt
     4141 tggctctttc tgcccttggt cactttgcac ttggaatgta attccttatg actaaatcat
     4201 taattttggt gagccatggt cgcttctgtg aggagcttag aggtagcaca gaaatgatta
     4261 tgggcccaca agacaacatt tacacagtag ctcttcttcc agaagatggc ccagaagaat
     4321 ttactgctaa atttgaagct gttattgaag gattggatga tttcctagtc tttgcggatc
     4381 ttctcggtgg gacaccttgt aatgtggtga gtcgcttgat catggaaggt cgtgatattg
     4441 acctttacgc agggatgaat cttccaatgg tgattgaatt tatcaatgcg agccttacag
     4501 gcgcagatgc ggactacaag agccgtgctg cagaaagcat tgtgaaagtt aatgacctgt
     4561 tagcgggctt cgatgatgac gaagatgaat aatactcttc gaaaatctct tcaaactacg
     4621 tcaacgtcgc cttgccgtag gtatatgtta ctgacttcgt cagtcttatc cggcaacctc
     4681 aaagcggtgc tttgagcagc ctgcggctag tttcctacag attttagttg gaactcgatt
     4741 caattcatgt gacaacgtga aaatcgttag agcattttat atagaatata catgggaatg
     4801 gagcttactc ccattcccat atttaataga aaaagaggaa ctcaatgcta cattatacaa
     4861 aagaagactt gctcgaattg ggtgcagaaa tcactacgcg tgaaatctac caacagcctg
     4921 atgtatggag agaagctttt gaattttatc aagcaaaacg tgaagaaatt gcagcctttc
     4981 tacaagaaat cgctgataaa catgactata ttaaggttat cttgacaggt gctgggactt
     5041 ctgcttatgt gggagatacc ttgctacctt actttaagga agtctatgac gaacgcaaat
     5101 ggaatttcaa tgctattgcg acaacagata tcgttgccaa accagcaacc tatttgaaaa
     5161 aagatgtggc aactgtcctt gtgtcttttg ctcgtagtgg gaactcgcct gaaagtgtgg
     5221 cgactgttga tttggccaaa tccttggtgg atgagcttta tcaagtgacg attacttgtg
     5281 cagcagatgg taaattggct cttcaagctc acggtgatga ccgtaatctc ttgctcttgc
     5341 aaccagctgt ctctaatgat gctggatttg ccatgacttc tagctttacg tctatgatgt
     5401 tgacagctct cttggtcttt gatcctacag aatttgctgt taagtctgaa cgttttgaag
     5461 ttgtatctag tcttgcccgt aaagttttag acaaggtaga agatgtcaaa gagctcgttg
     5521 atttagactt taaccgtgtc atctatctag gcgctggtcc tttctttgga cttgctcatg
     5581 aagctcagct caagattttg gaattaactg ctggtcaagt tgcgaccatg tatgaaagcc
     5641 cagttggctt ccgtcacggt ccaaaatctc ttatcaacga caatacagtt gttttggtct
     5701 ttggtacaac gacagcctac actcgtaagt acgacttgga cttggttcgt gaagttgctg
     5761 gtgaccagat tgctcgtcgt gttgtgcttt tgagtgatca agcttttggt cttgaaaatg
     5821 tcaaagaagt ggtccttggt tgtggcggtg tcttgaatga tatttaccgt gtcttccctt
     5881 acatcgttta tgcccaactc tttgctctat tgacttcact caaggtagaa aataaaccag
     5941 atacaccgtc tcctacaggt acagtaaacc gtgtagtaca aggtgtcata attcacgaat
     6001 atcaaaagta agacagtgtt tatgaattct tgacaagagg atttgtaaat tatcagataa
     6061 accatagatt gtcagtacgc tttctatggt ttgtttgctt gagagaaata gtaaaaggag
     6121 aacagaatga aagcatacac agagcgtgta tttggaaatg ttgagggcga ggatgtcttg
     6181 gcctatcgat ttgagacaga cggtggctac caacttgagg ttatgactta tggcgcgacc
     6241 atcttgcgct atgtcgcacc tgacaaggct ggaaattttg ccaatgttat cttgggattt
     6301 gatgactttg atagttatgt aggcaatagt cccaagcatg gagcaagtgt aggtcctgta
     6361 gcgggtcgta ttgcaggtgc gacctttgag ctcaatggta agacctatga ccttgaggtt
     6421 aataatgcta gcaactgtaa tcacagtggt tcaactggtt gggattccag cttgtttgaa
     6481 gttgaagaag taagcgatca tggcttgact ctctacacag agcgtacaga tgggacagga
     6541 gggttccctg gaaatctcaa gatttggatc agttatcact tggaagaaac tggtgcctat
     6601 gaaatcatct acaaggtaac gaccgatcag gatacgctgg tcaatccaac caaccacagc
     6661 tatttcaact tgtctggtga tttcacgcag acgattgacc gtcatgtctt ccaactaaac
     6721 acagagggca tttactcaat cgctcctgac ggtgttcctg ccaaaactcc agaagccaac
     6781 cgtgatgtgg tcaaacacat ctacaatggt accttgttga aggatatctt tgcagaagaa
     6841 gatgagcaaa tccagctggc atcaggtttg gatcacccat ttgcccttcc tgcaggccat
     6901 gacaatgctg gattccttta tgaccaaaat tcaggtcgct tcctgctttt caagacagaa
     6961 gctccttgct ttgtggtcta cacagcaaac tttgtggatg aaagtgtcat cataggaggt
     7021 cagccaatgc tacagcacaa tgggattgct cttgaagcgc aagctttacc agatgccatt
     7081 cacagtgacc ttaaaggcca agtcattctt aaagctggtc aaaccttcac cagtaagaca
     7141 cgttatgaac ttgttgtgaa ataaaaaaag ctggtcaaaa agtctttaaa attaaaataa
     7201 cgcatattgt caggtgatga aaagtttgat aatatgcgtt ttattatgaa atgatttact
     7261 ttatcttatc ctgaaatggt gttatttttg agttatctgg cttgactttc ttgatggaaa
     7321 ttatataata gttgtaataa caataattac aacgagaaaa gaggaccatt tatgacctat
     7381 gaatacaaga gtcacattta tttggcagag acagttttaa atgtaaagga tttggccagt
     7441 caaacagcct tttatcagca agttattggt ttagaaatct tatctcaaac tgagacagag
     7501 tccattctag gccttggtgg aaaagtcttg gtacagctga ttcaagcaca agagagcgga
     7561 gaagtgaggg aacattatgg tctttaccat ttggccattc tcttgcccac acgcaaggct
     7621 ttggctgatg tcttgaaaca cctgacagat ttgcaaattc ctcttgtcgg cggtgcagac
     7681 catggttaca gtgaagctat ttacttggaa gatttggagg gaaatggcat tgaactctat
     7741 cgagataagc cagtttccac atggaatatt cgagaagatg gacgcattat cggagtgacg
     7801 gaggctcttg cggcgcagga catttatgag ttgggggaaa gagtagagcc ctttatcctg
     7861 gcagaaggta caagaatggg gcatattcat ctttccgtca aggatagtcg aaagtccagc
     7921 cagttttatc aaaaggtgtt agggctagag aataaattca gtgtgcctgg cgctagttgg
     7981 attgcggctg gagattacca tcatcattta gcagttaacg aatggggagg aaaaggtctg
     8041 gcttcgcgta agcaaggctt accaggttta gcctactatg tcatcgaagt cgcacataaa
     8101 aaagaactgt taacgattgc ccaacgagca caagaagttg acgcaccaat caaatggatg
     8161 acatcgatcc aattggaaat cacagactca gatggcatcg tgacccgtat tcgtttagca
     8221 aggtaaaatt aggaattttt aataagttat aagtattgct agtttgcttt ttgaacggtc
     8281 tttggtaaca gaactgcgtg agctggatgg gtgagaattt tgacaaagaa gctgattaga
     8341 gatataatga agaaaaatcg agcaaggaaa aagaaatgac aaattcaaag tatattactt
     8401 gtttgaaacg ttcagagggc cagttgtgtg ggattcaaaa gatgattgaa ggagattgtg
     8461 actgtgctga tattgtgacg caactgacag cagtgagatc tagtgtagag cgcgtgattg
     8521 agatgataat taccgaaaat cttactgaat gtatcaatca gccactagat gattctgaag
     8581 ctcaaaagga acgcctagaa aaggctatcc gatacttgat taaacggaaa taaatgaatg
     8641 ttttgagacg atatgtgcaa aggtctatta actattagaa tagtttaatg tgagtcaaaa
     8701 gaagttgtaa aaaatcttta aaattaaaaa aacgcatagt atcaggtgtt gaatgtattg
     8761 accccaaaaa gttagatttt ttctgtctaa cttttggggt gcagttcata agaaccttgg
     8821 taatatgcgt tttattatgt agagacttat actcttcgaa aatctcttca aaccacgtca
     8881 gcttcacctt gccgtagata tggttactga cttcgtcagt tctatccaca acctcaaaac
     8941 agtgttttga gctgacttcg tcagttccat ccacaacct
//
LOCUS       DAVLYV010000079         8810 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLYV010000079 DAVLYV010000000
VERSION     DAVLYV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.079"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            98..5038
                     /locus_tag="VYO70_001746"
     CDS             98..5038
                     /locus_tag="VYO70_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1713939.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHTRENKLEKAEGVATASETAEAASAAKPEEK
                     ASEAVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEKEVQPDVPKNTEKT
                     LKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNEKASKEAKVQALSN
                     TNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDAGHRNGVPVYGTLF
                     FNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFINQETTGDLVKPLG
                     EKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYNYQFMQPEGDKVPA
                     DNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKTKVKWNDILDENGK
                     LRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGDKDWYGIANLVADR
                     TPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWRWWQTSTGEKLRAE
                     YDFTDAYNGGNSLKFSGDVAGKTVQDVRLYSTKLEVTEKTKLRVAHKGGKGSKVYMAF
                     STTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFFEHEGAVKDYQFNL
                     GQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADFYEVYEKDGDSWKL
                     LTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTTFDWGMTVKDTSLP
                     KPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSAQLSGSVDIRLTKP
                     RTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEVRGNKAHVTDITLD
                     KPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMAKAAARSLGNNKVQ
                     VGFSDVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQSGLLYYRTQLPGK
                     EISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLRVQYEGGTEDELIR
                     LTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGKDEASPKTILGIEVSQEP
                     KKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQKTGRQTLTLHYQGH
                     EVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEAFLKAIEAAEQVLK
                     DHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQELLAAKPNHPSGS
                     ALAPLLEKNKALVEKVDLSPEELTTAKQSLKDLVALLKEDKPAVFSDSKTGVEVHFSN
                     KEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVDLSYASIVKIPIEK
                     DKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESAEKPQPAKPAPQNT
                     VLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRVIIQGRDGLLRHVF
                     EVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQNTAVKSEEASKQL
                     PNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            5128..6324
                     /locus_tag="VYO70_001747"
     CDS             5128..6324
                     /locus_tag="VYO70_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1713940.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINVLEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            6460..6987
                     /locus_tag="VYO70_001748"
     CDS             6460..6987
                     /locus_tag="VYO70_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1713941.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            7064..7420
                     /gene="glnR"
                     /locus_tag="VYO70_001749"
     CDS             7064..7420
                     /gene="glnR"
                     /locus_tag="VYO70_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1713942.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            7457..8803
                     /gene="glnA"
                     /locus_tag="VYO70_001750"
     CDS             7457..8803
                     /gene="glnA"
                     /locus_tag="VYO70_001750"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1713943.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
BASE COUNT         2817 a         1624 c         1973 g         2396 t
ORIGIN      
        1 ggataaaaat gtgattatca tggttctaga gttaaaaaac cacagaatat aaagcgcttt
       61 cttaaaaaca acaaaatcaa tcttttagga ggagaaaatg aagaatccat tttttgaaag
      121 acgttgtcgt tacagtattc gtaagttatc agtaggagcc tgctcgctga tgattggtgc
      181 tgttttattt gctggtccag ccttggctga agaaactgca gttcctgaaa atagcggagc
      241 taatacagag cttgtttcag gagagagtga gcattcgacc aatgaagctg ataagcagaa
      301 tgaaggggaa catactagag aaaacaagct agaaaaggca gaaggagtag cgacagcatc
      361 tgaaactgca gaagcagcta gcgcagctaa accagaggaa aaagcaagtg aggcggttgc
      421 agaaacacca tctgcagaag caaaacctaa gtctgacaag gaaacagaag caaagcccga
      481 agcaactaac caaggggatg agtctaagcc agcagcagaa gctaataaga ctgaaaaaga
      541 agtccagcca gatgtcccta aaaatacaga aaaaacatta aaaccaaagg aaatcaaatt
      601 taattcttgg gaagaattgt taaaatggga accaggtgct cgtgaagatg atgctattaa
      661 ccgcggatct gttgtcctcg cttcacgtcg gacaggtcat ttagtcaatg aaaaagctag
      721 caaggaagca aaagttcaag ccttatcaaa cactaattct aaagcaaaag accatgcttc
      781 tgttggtgga gaagagttca aggcctatgc ttttgactat tggcaatatc tagattcaat
      841 ggtcttctgg gaaggtctcg taccaactcc tgacgttatt gatgcaggtc accgtaacgg
      901 ggttcctgta tacggtacac tcttcttcaa ctggtctaat agtattgcag atcaagaaag
      961 atttgctgaa gctttgaagc aagacgcaga tggtagcttc ccaattgccc gtaaattggt
     1021 agacatggcc aagtattatg gctatgatgg ctatttcatc aaccaagaaa caactggaga
     1081 tttggttaaa cctcttggag aaaagatgcg ccagtttatg ctctatagca aggaatatgc
     1141 tgctaaggta aaccatccaa tcaagtattc ttggtatgat gccatgacct ataactatgg
     1201 acgttaccat caagatggtt tgggagaata caactaccaa ttcatgcaac cagaaggaga
     1261 taaggttccg gcagataact tctttgctaa ctttaactgg gataaggcta aaaatgatta
     1321 cactattgca actgccaact ggattggtcg taatccttat gatgtatttg caggtttgga
     1381 attgcaacag ggtggttcct acaagacaaa ggttaagtgg aatgacattt tagacgaaaa
     1441 tgggaaattg cgcctttctc ttggtttatt tgccccagat accattacaa gtttaggaaa
     1501 aactggtgaa gattatcata aaaatgaaga tatcttcttt acaggttatc aaggagaccc
     1561 cactggccaa aaaccaggtg acaaagattg gtatggtatt gctaacctag ttgcggaccg
     1621 tacgccagcg gtaggtaata cttttactac ttcttttaat acaggtcatg gtaaaaaatg
     1681 gttcgtagat ggtaaggttt ctaaggattc tgagtggaat tatcgttcag tatcaggtgt
     1741 tcttccaaca tggcgctggt ggcagacttc aacaggggaa aaacttcgtg cagaatatga
     1801 ttttacagat gcctataatg gcggaaattc ccttaaattc tctggtgatg tagccggtaa
     1861 gacagttcag gatgtgagac tttattctac taagttagaa gtaactgaga agaccaaact
     1921 tcgtgttgcc cacaagggag gaaaaggttc taaagtttat atggcattct ctacaacttc
     1981 agactacaaa ttcgatgatg cagatgcatg gaaagagcta accctttctg acaactggac
     2041 aaatgaagaa tttgatctca gctcactagc aggtaaaacc atctatgcag ttaaactatt
     2101 tttcgagcat gaaggtgctg taaaagatta tcagttcaac ctcggacaat taactatctc
     2161 ggacaatcac caagagccac aatcgccgac aagcttttct gtagtgaaac aatctcttaa
     2221 aaatgcccaa gaagcggaag cagttgtgca atttaaaggc aacaaggatg cagatttcta
     2281 tgaagtttat gaaaaagatg gagacagctg gaaattacta actggctcat cttctacaac
     2341 tatttatcta ccaaaagtta gccgctcagc aagtgctcag ggtacaactc aagaactgaa
     2401 ggttgtagca gtcggtaaaa atggagttcg ttcagaagct gcaaccacaa cctttgattg
     2461 gggtatgact gtaaaagata ccagcctacc aaaaccacta gctgaaaata tcgttccagg
     2521 tgcaacagtt attgatagta ctttccctaa gactgaaggt ggagaaggta ttgaaggtat
     2581 gttgaacggt accattacta gcttgtcaga taaatggtct tcagctcagt tgagtggtag
     2641 tgtggatatt cgtttgacca agccacgtac cgttgttaga tgggtcatgg atcatgcagg
     2701 ggctggtggt gagtctgtta acgatggctt gatgaacacc aaagactttg acctttatta
     2761 taaagatgca gatggtgagt ggaagctagc taaggaagtc cgtggcaaca aagcacacgt
     2821 gacagatatc actcttgata aaccaatcac tgctcaagac tggcgcttga atgttgtcac
     2881 ttctgacaat ggaactccat ggaaggctat tcgtatctat aactggaaaa tgtatgaaaa
     2941 gcttgatact gagagtgtca atattccgat ggccaaggct gcagcccgtt ctctaggcaa
     3001 taacaaggta caagttggct tttcagatgt accggctgga gcaactatta ctgtttatga
     3061 taatccaaat tctcaaactc cgcttgcaac cttgaagagc gaagttggag gagacctagc
     3121 aagtgcacca ttggatttga caaatcaatc tggtcttctt tattatcgta cccagttgcc
     3181 aggcaaggaa attagtaatg tcctagcagt ttccgttcca aaagatgaca gaagaatcaa
     3241 gtcagtcagc ctagaaacag gacctaagaa aacaagctac gccgaagggg aggatttgga
     3301 ccttagaggt ggtgttcttc gagttcagta tgaaggagga actgaggacg aactcattcg
     3361 cctaactcac gcaggtgtat cagtatcagg ttttgatacg catcataagg gagaacagaa
     3421 tcttactctc caatatttgg gacaaccggt aaatgctaat ttgtcagtga ctgtcactgg
     3481 caaagacgaa gcaagtccga aaactatttt gggaattgaa gtaagtcagg aaccgaaaaa
     3541 agattaccta gttggtgata gcttagactt gtctgaagga cgctttgcag tggcttatag
     3601 caatgacacc atggaagaac attcctttac tgatgaggga gttgaaattt ctggttacga
     3661 tgctcaaaag actggtcgtc aaaccttgac gcttcattac caaggccatg aagttagctt
     3721 tgatgttttg gtatctccaa aagcagcatt gaacgatgag tacctcaaac aaaaattagc
     3781 agaagttgaa gctgctaaga acaaggtggt ctataacttt gcttcaccag aagtaaaaga
     3841 agctttcttg aaagcaattg aagcggccga acaagtgttg aaagaccatg aaactagcac
     3901 ccaagatcaa gtcaatgacc gacttaataa attgacagag gctcataaag ctctgaatgg
     3961 tcaagagaaa tttaaggaag aaaagacaga acttgatcgc ttaacaggtg aggttcaaga
     4021 actcttggct gccaaaccaa accatccttc aggttctgcc ctagctccgc ttcttgagaa
     4081 aaacaaggcc ttggttgaaa aagtagattt gagtccagaa gagcttacaa cagcgaaaca
     4141 gagtctaaaa gatctggttg ctttattgaa agaagacaag ccagcagtct tttctgatag
     4201 taaaacaggt gttgaagtac acttctcaaa taaagagaag actgtcatca agggtttgaa
     4261 agtagagcgt gttcaagcaa gtgctgaaga gaagaaatac tttgctggag aagatgctca
     4321 tgtctttgaa atagaaggtt tggatgaaaa aggtcaagat gttgatctct cttacgcttc
     4381 tattgtgaaa atcccaattg aaaaagataa gaaagttaag aaagtatttt tcttacctga
     4441 aggcaaagag gcagtagaat tggcttttga acaaacggat agtcatgtta tctttacagc
     4501 acctcacttt actcattatg cctttgttta tgaatctgct gaaaaaccac aacctgctaa
     4561 accagcacca caaaacacag tccttccaaa acctacttat caaccggctt ctgatcaaca
     4621 aaaggctcct aaattggaag ttcaagagga aaaggttgcc tttcatcgtc aagagcatga
     4681 aaatgctgag atgctagttg gggaacaacg agtcatcata cagggacgag atggactgtt
     4741 aagacatgtc tttgaagttg atgaaaacgg tcagcgtcgt cttcgttcaa cagaagtcat
     4801 ccaagaagcg attccagaaa ttgttgaaat tggaacaaaa gtaaaaacag taccagcagt
     4861 agtagctaca caggaaaaac cagctcaaaa tacagcagtt aaatcagaag aagcaagcaa
     4921 acaattgcca aatacaggaa cagctgatgc taatgaagcc ctaatagcag gcttagccag
     4981 ccttggtctt gctagtttag ccttgacctt gagacggaaa agagaagata aagattaaat
     5041 atcgaaaaat cttgtgaaat ctttccttat atttccaaag tgtgatataa tagtttcgaa
     5101 taaaataaat aaaggagttt ttgtaacatg gcaaaactta ctgttaaaga cgttgacttg
     5161 aaaggtaaaa aagtcctcgt tcgtgttgac ttcaacgtac cattgaaaga tggcgtaatc
     5221 actaacgata accgtatcac agcagctctt ccaactatta agtacatcat cgaacaaggt
     5281 ggacgtgcaa ttcttttctc tcaccttgga cgtgtgaaag aagaagctga taaagctggt
     5341 aaatcacttg ctcctgtagc agctgacttg gcagcaaaac ttggtcaaga tgttgttttc
     5401 ccaggtgtca ctcgtggtgc tgaattggaa gcggcaatca acgttcttga agatggacaa
     5461 gttctcttgg ttgaaaacac tcgttacgaa gatgttgacg gcaagaaaga atctaaaaac
     5521 gatcctgaac ttggtaaata ctgggcatca cttggagatg gtatcttcgt aaacgatgca
     5581 ttcggtacag ctcaccgtgc acacgcatct aacgttggta tctcagcaaa cgttgaaaaa
     5641 gcagttgctg gtttccttct tgaaaacgaa attgcctata tccaagaagc agttgaaact
     5701 ccagaacgtc cattcgtggc tatccttggt ggttcaaaag tttcagacaa gatcggtgtt
     5761 atcgaaaact tgcttgaaaa agctgataaa gtccttatcg gtggtgggat gacttacaca
     5821 ttctacaaag cacaaggtat cgaaatcggt aactcacttg tagaagaaga caaattggat
     5881 gttgcgaaag ctcttcttga aaaagcaaat ggtaaattga tcttgccagt tgactcaaaa
     5941 gaagctaacg catttgctgg ttacactgaa gtgcgtgaca ctgaaggtga agcagtttct
     6001 gaaggcttcc ttggtcttga catcggtcca aaatctatcg ccaaatttga cgaagctttg
     6061 actggtgcca aaacagttgt atggaacgga cctatgggtg tatttgaaaa cccagatttc
     6121 caagctggta caatcggtgt gatggacgct atcgtgaaac aaccaggagt taaatcaatc
     6181 atcggtggtg gtgactcagc tgccgcagcg attaaccttg gccgtgcaga caagttctca
     6241 tggattagta cgggtggtgg agcatcaatg gaacttcttg aaggtaaggt tcttccagga
     6301 cttgcagcct tgacagaaaa ataagatttt ataaacaaat caaagaagag agggatgaaa
     6361 gttcctcttt tcttttgctt aaaataaaaa cgcttcctct caactattac tcataaaaat
     6421 caccgattta tgataaaatg gaaatagaaa gttgagatta tgagttattt taaaaaatat
     6481 aaattcgata aatcccagtt caaacttggt atgcgaacct ttaaaacagg tattgctgtt
     6541 tttctagttc tcttgatttt tggctttttt ggctggaaag gtcttcaaat tggtgctttg
     6601 acagccgttt ttagcctgag ggagagtttt gatgagagtg ttcattttgg gacttcgcgt
     6661 attctaggaa atagtatcgg tggactctat gccttggtct tcttcttatt aaataccttt
     6721 ttccacgaag ccttttgggt gaccttggta gttgttccaa tctgcaccat gttaaccatt
     6781 atgacaaatg tagccatgaa taacaaagca ggggttattg gtggtgtagc agctatgtta
     6841 atcattaccc tatcaattcc aagtggtgag acaattttgt acgtgtttgt gcgtgtatta
     6901 gaaacgttta tgggagtttt tgtcgcaatt atcgtaaatt acgatattga tcgtattcgt
     6961 ctctttttag agaaaaaaga aaaataatgt tacattttat aacattatca attgacgttt
     7021 gtcttttttt agactataat agacagaaag aaggaaattg taaatgaagg aaaaagaatt
     7081 tcgccgaaat atggctgttt ttcctatcgg cagtgttatg aagttgaccg atctatcggc
     7141 gcgtcagatt cgttattatg aagatcaaga gttgatcaag cccgatcgaa acgaaggaaa
     7201 tcgtcgcatg tattccttga atgacatgga tcgtctgctt gaaatcaaag attatatctc
     7261 tgaaggttat aatatcgctg ccattaagaa aaaatatgct gaacgtgaag cgaaatccaa
     7321 gaaagcggtt agtcagactg aagtacgtcg tgcacttcac aatgaactcc tccaacaggg
     7381 gcgttttgct tcagtacagt caccttttgg tcgcggttag gcaaccgcaa gtagtcatag
     7441 attaaggaga aaacttatgc caatcacagc tgcagatatt cgtcgtgaag tcaaggaaaa
     7501 aaatgttacc tttattcgtc ttatgttctc agatattttg ggaaccatga aaaacgtcga
     7561 aattcctgct acagatgaac agttagataa ggtcttgtcg aacaaggtta tgtttgatgg
     7621 atcttctatt gaaggttttg tacgtatcaa tgagtcggat atgtacttgt acccggactt
     7681 ggatacatgg acagtcttcc cttggggaga tgaaaatgga agtgttgcag gtctgatctg
     7741 tgatgtttat acaacagaag gtgaaccatt tgcgggtgac cctcgtggta atttgaaacg
     7801 agctcttcgt cacatggaag aagttggatt caaatccttc aaccttggtc cagagccaga
     7861 attcttccta tttaagttgg atgaaaatgg ggacccaaca cttgaagtga atgacaaggg
     7921 tggctacttt gatttggcac ctactgacct tgcggacaac acacgtcgtg agattgtgaa
     7981 tgtcttgacc aaaatgggat ttgaagtaga agcgagtcac cacgaggttg cggttggaca
     8041 gcatgagatt gactttaagt acgatgaagt tctccgtgct tgtgataaga ttcaaatctt
     8101 taaacttgtt gttaaaacca ttgctcgcaa acacggactt tacgcaacat ttatggcgaa
     8161 gccaaaattt ggtattgctg gctcaggtat gcactgtaat atgtccttgt ttgatgcaga
     8221 aggaaataac gccttctttg atccaaatga tccaaaagga atgcagttgt cagaaacagc
     8281 ttaccatttc ctaggcggtt tgatcaagca tgcttacaac tatactgcca tcatgaaccc
     8341 aacagttaac tcatacaaac gtttggttcc aggttatgaa gcgcctgttt acattgcttg
     8401 ggctggtcgt aaccgttcgc cacttgtgcg cgtacctgct tcacgtggta tgggaactcg
     8461 tcttgagttg cgttcagtgg atccaatggc gaacccttac attgctatgg ctgttctttt
     8521 ggaagttggt ttgtatggta ttgaaaataa aatcgaagca ccagctccta tcgaagaaaa
     8581 tatctacatc atgacagcag aagagcgcaa ggaagctggt attacagacc ttccatcaac
     8641 tcttcacaac gctttgaaag ctttgacaga agatgaagtg gttaaagctg ctctcggaga
     8701 tcacatctac actagcttcc ttgaagccaa acgaatcgaa tgggcaagtt atgcaacctt
     8761 cgtttcacaa tgggaaattg ataattattt agacctttac taatatagaa
//
LOCUS       DAVLYV010000080         8433 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLYV010000080 DAVLYV010000000
VERSION     DAVLYV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8433
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.080"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(61..897)
                     /gene="folK"
                     /locus_tag="VYO70_001751"
     CDS             complement(61..897)
                     /gene="folK"
                     /locus_tag="VYO70_001751"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1713944.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKKGSLSTV
                     VG"
     gene            complement(940..1494)
                     /gene="folE"
                     /locus_tag="VYO70_001752"
     CDS             complement(940..1494)
                     /gene="folE"
                     /locus_tag="VYO70_001752"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1713945.1"
                     /translation="MDRQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(1487..2797)
                     /locus_tag="VYO70_001753"
     CDS             complement(1487..2797)
                     /locus_tag="VYO70_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1713946.1"
                     /translation="MKEFENNQWITNYRTDQPHFGLERMVELLALRGNPHLKLKVIHI
                     GGTNGKGSTITFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGDSIPEARLEALMADY
                     QSLLEGEATANLQGTTEFEIITALAYDYFAAEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGVPLVTGRISPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKARNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENG"
     gene            complement(2799..3779)
                     /gene="folP"
                     /locus_tag="VYO70_001754"
     CDS             complement(2799..3779)
                     /gene="folP"
                     /locus_tag="VYO70_001754"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1713947.1"
                     /translation="MMKSLEGVTDMSSKANHAKTAICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISI
                     DTWKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKARAKVVIMFNPVMARPQHPSS
                     LIFPHFGFGQTFTEKELADFETLPIEDLMEAFFDRALSKADEAGIAPENILLDPGIGF
                     GLTKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETEHGFRNWDT
                     ASAHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(3875..4582)
                     /locus_tag="VYO70_001755"
     CDS             complement(3875..4582)
                     /locus_tag="VYO70_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1713948.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(4635..6053)
                     /locus_tag="VYO70_001756"
     CDS             complement(4635..6053)
                     /locus_tag="VYO70_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1713949.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLVITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAAGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            6243..7055
                     /locus_tag="VYO70_001757"
     CDS             6243..7055
                     /locus_tag="VYO70_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1713950.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            7394..8413
                     /gene="adhP"
                     /locus_tag="VYO70_001758"
     CDS             7394..8413
                     /gene="adhP"
                     /locus_tag="VYO70_001758"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1713951.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         2504 a         1964 c         1653 g         2312 t
ORIGIN      
        1 atcgctctga atatcaaaaa agaaaggaca aaatttgtcc tttctcgagc ttagctgact
       61 tcaacccact acagttgaca aagagccttt tttcaaagca tcatacaagt tgcggatcgg
      121 ttgttttaat atcggatgga taaaatgagg cgcaatttcc tgtaaggact caaggacaaa
      181 aaggcgttcc gctatgtaag gatgaggcaa tatgaggtcg tctgtataaa ggatctggtc
      241 ctccacaaag agcaagtcca aatcaatcaa acgaggtccc caatgcactt ctctcacccg
      301 tcccagctct gactcaatgg ctaacaaggt ttctaacaag tcttgtgctg gtagccaggt
      361 ttccacctca accacttgat tggcaaagct atcctgctcc actccacccc aaggctccgt
      421 cgctaagaca ctggactctt tgagaatatg gatgccacga gctcgcagtt tgtcaatggc
      481 ttgcttcaag tttgcttgtt tatctcccat attgcttcct agggcgataa aggctcgttg
      541 cttgcggcga tgaatggtta ccgagcaagt atctagtgac aaatgcaccg gtgcccaagg
      601 ttttttcagt tccaacttca tttcttggac aagaggataa gactcaaagg tacgttccac
      661 cagtttatag gctaccgttt caatcaaatc ttcactcgtt tcctgaaacc aagtcgtcca
      721 ctgctgacac aattctccgt aatggacaga ggctgttaaa tccaagtctg tagctgcctt
      781 ggtcatatca taggatagga tggctgaaac gacaaatttc tgccccaatt ctttctcact
      841 aggaaaaaga ccatgatagg caaacatttc caaatcctta atctgcagtt gatccataat
      901 gattcctttc tagaaaaatc cgcttgaagc ggattctttt tatagcccca ttaaacgata
      961 agcttggtca cggagatcct tatctgtttc aaatagacca cgagctactg tcgtcaaggt
     1021 tgcagtgcct ggttttctaa caccacgcat actcatacac atatgttccg cctcaatgac
     1081 aacaaaggct cctttagcac ctagatagtc catcaaggca tcggccactt cgatattcaa
     1141 acgttcttga atttgtggtt ttttcgaata aacttcaacc gtacgggcta gcttagacaa
     1201 gcctgccaca cgaccatctg gaatgtaggc aatgtgcgct ctaccataaa atggcaagaa
     1261 gtggtgttca cacatggtat ggaaaaagat atccttttct accaccatat tatcgtcaat
     1321 aatttcaaag gattttgaca aatgttcctc tgctgtttga ccaagacctg aaaaaatctc
     1381 ttgatacata cgggctacac gagcaggtgt ttcctgcaag ccctcgcgat tagcgttctc
     1441 tcctacagcc tcgataatca tttttacagc tgcttcaatc ttttgtctat ccattctcgt
     1501 tcttcctctc catcagatag gccctcactt ggctcaagaa atacaaggaa cctgtgacaa
     1561 tcctaactgt ttgtttctct tcttttttat ctgtcaaatt ctgctctaga aaatcatgcc
     1621 aatcttggta gctgagattt ctagccttag ctgcctcttt cagcacgctt tcatccgtcg
     1681 cccgactatc cgcaaaatgt gttagagtaa gctcggtatc tggcatggct cccagcaagt
     1741 ccaacatatc ctccaaggct ttggttttga tacaagtgaa gaggatttcc ttatgataat
     1801 ccgcaaaacg ttcttgcaag gttaccaaca aggccttgat agcatgggga ttatgggctc
     1861 catccaaaat catcaaggga tctcttgaca cgatttccaa acgccctggc caacttgttt
     1921 cttccaaggc ttgaccaaga aaatcattgc ttgctagctc tcgaccatct tcttgacaaa
     1981 aagtatcaag taaagctatg gccatcccag cattctctat ttggtacaaa ccaagcaggc
     2041 tagtctggaa gcgaccttgt ctgacagcac ttgtatagtc aaagacttcc cctgtcacca
     2101 cactttcttg atgacgaacc tgataatctg tcccgtaggc aagtctcggc gcatctttcc
     2161 cttccgcaat gcggtcaatc acagccaaag cttctggaga aatgcgacct gtcaccaagg
     2221 gaactccttg tttgataata ccagctttct gctctgctat ggcctccaag gtgtcaccaa
     2281 gtagggccac atgatccaag ccaatggtcg tgatgcctgt tagaataggc tggcagacat
     2341 tggtactatc taaaagtcca cccatgccaa cttccatgat ggccacatct acttgctctg
     2401 cagcaaagta gtcataggcc agggctgtga taatctcaaa ctcggttgtc ccctgtaaat
     2461 tagcggtcgc ttctccctcc agcaaagact gatagtctgc catgagggct tctagcctag
     2521 cctctggaat ggagtcccca ttgatgctaa tctggtctgt gtaatgaatg agatagggcg
     2581 agctaaacac gccaactctc agccctagct tttctagcat ctttttcaaa aaagtaatgg
     2641 tcgaaccctt gccgttagtc cctccgatat ggatgacctt gagtttgaga tggggattgc
     2701 cacgcaaagc taacagttcc accattcgtt ccaagccaaa atgcggttgg tccgtccggt
     2761 aattggtaat ccactgattg ttttcaaatt ctttcatctt atttgtattg ttttaaatct
     2821 aaattttccg cttcatcagc cagacgaata gcggaggcaa tttcaactgc catcctgtga
     2881 ctagctacgt catgcacgcg caccacttct acaccctgtc tcgcagcgat actagttaca
     2941 tgagccgaag ctgtgtccca attgcggaaa ccatgttctg tctcaggatt gacttcaaac
     3001 ccactttctt caaggatatt gatgacaaat cgcttgcgcg aaactccgag aaagattgga
     3061 tagcctttct gatggagttt atccagatcc cgtaaaagaa gcagattttc tttcttagtc
     3121 agaccaaagc caattcctgg atccagcagg atattttctg gtgcaatacc agcttcgtcc
     3181 gctttcgata gggctctgtc aaagaaagcc tccatcaagt cttcgattgg caatgtttca
     3241 aagtcagcta actctttttc tgtaaaggtt tgaccaaagc caaaatgagg gaagataagc
     3301 gaactaggat gctgaggtcg agccataact ggattaaaca tgatgaccac tttcgctctc
     3361 gctttagcta ccacatgagc cattttttca tcacccatga gaccagtgat atcattaact
     3421 agattagcac cagcagccaa agcagcttct gctacctgac tcttccaagt atcaatagag
     3481 atgaggacat cactttcctt gcgaatcgct ttaatcactg gaacaacacg ctggatttcc
     3541 tcttctatct caacatagct acttcccggc cgcggccgag tcgattctcc accgatatct
     3601 agcatgctag ccccttctgc tatcaattta cgagcctgct ggagtgcctg ctcaagagca
     3661 aaaaattgac caccatccga aaaggagtct ggggttacat tgataattcc gcaaatagct
     3721 gtctttgcat gattggcttt acttgacata tcggtcactc cctcaaggct tttcatcata
     3781 ttatttctct attttaccat aaaaagaaaa agatggacac gattgcattc atctttttcc
     3841 cagtagaaag aggtcggttc ctacataaaa acttttaaac agaaattcct aatgtccgac
     3901 tcataatcac cacaagagcc aacaaacaga aagcaatccc attaacaatc atgtgaagca
     3961 agatcgacat ttccaaacgt tgggtcttgt aggctgtcca agatagaact gtcgacatac
     4021 ctccataaat caataaagaa ggtaaattac ttggttgatg caataaagca aacacaatcg
     4081 taccgactac aaatcccaag ttctccttgc ctcggaaaat ctttttagga acaatcccac
     4141 gacacaagat ttcctcacaa atcggagcaa gcaaggctag caagaagaaa ctggaaatca
     4201 acgaactatt ttgaaccata tcattaatct gagactggtt agctgttgtc gtctcatttg
     4261 acagttgcaa taaaatggaa ccaagtatat ttgacccgac aataactaga taactcaagc
     4321 ccaaacgtgc caaatcttta gctctaaaaa aagaaaaatt aaaactagct aacttggttt
     4381 tacgagctcc cataataaat agagccagaa ccacaattga aaggccagca actatcagcc
     4441 ctgactgtag cagggctact tcttttaaag tcaaaataga ggtaaccact aggggaacct
     4501 gatataaaag gacggcaagt aaaaagacca aaatccagcc tgcacgattc aacaattctt
     4561 tccacatatt tttctctttc atcacctatc tcctttgtat taaaaaaggg gggaaatccc
     4621 cctgggtcat tctattataa ggccatgctg atgtagttaa ggataaacaa ggcatccaaa
     4681 atccaaatca tggcatgaac atctttaact tgacctttga caagcttagt caaagtgtaa
     4741 gtcaagaaac caactgcaat cccttgagtg atagagtagc tgaatcccat aaagatagat
     4801 gtgaagaagg caggaactgc ttcagacata tcatcccaat ggatattttt caagctacca
     4861 agcatcataa tcccaacgat aatcaagatt ggagctgtag ccgctgttgg tacgatcgct
     4921 agaagtgggc taaagaagct tgaaatcgca aaacagatag ctacaaccaa ggctgtcaaa
     4981 ccagtacgtc cacctgcacc gataccagca gcagactcaa cataagtcgt tacgtttgaa
     5041 gtacctgcaa tggcaccgac tgtcgttcca atcaaatcag agtaaagagc cttatccaat
     5101 ttggctgatt ggtgattttc accatttgtc gctacaatac caactttttc acctgtaccg
     5161 atcaaggtac caattgtgtc aaaaatatct gtcaatgaga aggcaagaat ggccatcaga
     5221 gtttcaggca agcgagctgt atctgaaacc aaagctccca aaccttctga accaagagct
     5281 gcaccaaaga ttgtcttcaa atcttcaaaa gctgcaccaa catggttatt agcaaaatcg
     5341 atactagaca aatcaaccaa accagctgca atagcaagaa cagttgttgt caagatagag
     5401 agaataattc cccctttaat ccctttgatg acaaagaaga tagtaatggc aagtcctgca
     5461 agagccacta aaacagctgg attattaaag ctgaccaatc ctggaactgc tgaagagttt
     5521 gctgcaatcg ttgcttgagc tttgtcagcc ccttctccta caacagtata gttgcctgga
     5581 tcaatcgtaa atttcaaaag tccagcattc ttaatcccta cataggcaag gaagacaccg
     5641 ataccagctg aaatagccga gcgaagggca tttggaatcg attcaatgat cattttacga
     5701 acatttgtca aggtaataac caatgaaata atcccacaga tgaagaccat agctagggct
     5761 tcttgccatg aataaccaag tccaaatacg actgtaaagg taaagaaggc attgagtccc
     5821 atacctggtg cttgagcgta tggcaagtta gcatagaagg ccatcatcaa ggtaccagca
     5881 actgcaccga taatcgttgc aagaaacaca ccctgagcag gcattcctgt ttgtgaaagt
     5941 atttgtgggt ttacaaagag aatatagctc attgcaaaga aagttgttaa accagcgaga
     6001 acctctgtac gaacgtctgt accgttctct tttagtttaa ataatttgtc catcatcaat
     6061 ctccttttaa tttttacgaa caataataga attatatcat ttatcattca ctttttcaat
     6121 gtctttgttt gatttgttta agaaattaat ccaatttcat tttttgtttg gaatctgctt
     6181 ttctgcctgc tttctgttat aatagaagac atcttatctg aaaacacact agaaaggtcc
     6241 atatgacgaa aaaaattatt gcagttgacc tagatggaac cctgctcaac tcagacagtc
     6301 aaatttctga ctttaccaaa aaaaccatca aaaaagttgc tgaaaaaggc catcaggtta
     6361 ttattacgac aggtcgccct taccgtatgt caaaagattt ttaccgtgaa ctgggcttag
     6421 acactcctat gattaacttc aacggatccc ttactcattt accagaccaa gtttgggatt
     6481 ttgaaaagtg tttgactgta gacaaaaaat atctgctaga tatggttcaa cgttcagagg
     6541 acattcaagc cgattttatc gctggagaat atcgtaaaaa attctacatt acaaatccca
     6601 atgaagaaat tgccaatccc aaactatttg gtgtagaagc tttccagcct gaagatcaat
     6661 tccagcctga attggtgacc aaggacccta actgtatcct cttgcagacc agagccagtg
     6721 acaaatatgc cttggcaaaa gacatgaacg ccttctacca gcatcaactt tctatcaata
     6781 cctggggagg tccgctcaat atccttgaat gtaccccaaa aggtgtcaac aaggcctttg
     6841 ctttggacta cttgctcaag ataatgaatc gtgacaaaaa agatttgatt gcctttggag
     6901 atgaacacaa tgataccgaa atgctcgctt ttgctgggaa gggttatgcc atgaaaaatg
     6961 ccaatccaga gctactccct tatgcagatg agcaaatttc ccttaccaac gaccaagatg
     7021 gggttgccaa aaccctacaa gacttattct tataacctat actgatactc aatgagaatc
     7081 aaagagcaaa ctaggaagct agccgtaggt tgctcaaagc actgctttga ggttgtagat
     7141 gaagctgacg tggtttgaag agattttcga agagtatgac cagcttgcaa gctggtcttt
     7201 gtgctctttt cacagtctca tcaaccagtt aatcgattgt tctacttttt gtctccaaaa
     7261 ccttggaaaa ggctttcttt tgtgatatac ttcacatata aatacaagag agctcgtcac
     7321 actcgactct gttgacaaaa aatcaatcca gtcccgttgt ccctgttctc ggtcaatatc
     7381 aaggaggata gctatgaaag ctgttgttgt aaatccagaa agcactggtg ttgctattga
     7441 agaaaaagta ctccgtccac ttgaaactgg ggaagcactt gtagaagttg aatactgtgg
     7501 cgtttgccac accgacctcc acgttgctca tggtgacttt ggtcaagtcc caggacgtgt
     7561 tcttgggcac gaaggtatcg gtatcgttaa agagattgct ccagatgtga aaagccttaa
     7621 agtcggtgac cgcgtcagcg ttgcttggtt ctttgaagga tgtggcactt gcgaatactg
     7681 tacaactggt cgcgaaaccc tttgccgtac agtgaaaaat gctggctact cagtagacgg
     7741 tggtatggct gaacaatgta tcgtaactgc tgactatgct gtcaaggttc ctgacggact
     7801 tgatccagcc caagcttctt ctatcacatg tgctggagta acaacctata aagctatcaa
     7861 agaagcaaaa gttgaaccag gccaatgggt tgttctttac ggtgctggtg gacttggtaa
     7921 cctcgctgtt caatacgcta aaaaagtatt caatgctcat gttatcgcag tcgatatcaa
     7981 caatgacaaa cttgcccttg caaaagaagt aggcgctgac attgtgatta acggcctcga
     8041 agttgaagat gtagctggac tcatcaaaga aaaaactgat ggaggagctc attcagctgt
     8101 cgtaactgct gtgtctaaag ttgccttcaa ccaggctgtt gactccgttc gtgctggtgg
     8161 tcgcgtcgtc gctgttggtc ttccttctga aatgatggaa ctcagcatcg ttaaaacagt
     8221 cctcgatgga atccaagtca tcggttctct tgtcggaact cgtaaagact tagaagaagc
     8281 cttccaattt ggtgcagaag gtctggtagc cccagttgtt caaaaacgtc cagtagaaga
     8341 tgctgttgcc attttcgacg aaatggaaaa aggtcaaatc caaggacgta tggtactcga
     8401 cttcacccac taatctaata gaaacctatt ttc
//
LOCUS       DAVLYV010000081         8365 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLYV010000081 DAVLYV010000000
VERSION     DAVLYV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8365
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.081"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            118..516
                     /locus_tag="VYO70_001759"
     CDS             118..516
                     /locus_tag="VYO70_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1713952.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            984..1937
                     /locus_tag="VYO70_001760"
                     /pseudo
     CDS             984..1937
                     /locus_tag="VYO70_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155340.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            2012..2212
                     /locus_tag="VYO70_001761"
     CDS             2012..2212
                     /locus_tag="VYO70_001761"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1713953.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            2247..2963
                     /gene="atpB"
                     /locus_tag="VYO70_001762"
     CDS             2247..2963
                     /gene="atpB"
                     /locus_tag="VYO70_001762"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1713954.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKIQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            2977..3471
                     /gene="atpF"
                     /locus_tag="VYO70_001763"
     CDS             2977..3471
                     /gene="atpF"
                     /locus_tag="VYO70_001763"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1713955.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            3471..4007
                     /locus_tag="VYO70_001764"
     CDS             3471..4007
                     /locus_tag="VYO70_001764"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1713956.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            4022..5527
                     /locus_tag="VYO70_001765"
     CDS             4022..5527
                     /locus_tag="VYO70_001765"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1713957.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            5543..6421
                     /locus_tag="VYO70_001766"
     CDS             5543..6421
                     /locus_tag="VYO70_001766"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1713958.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            6507..7913
                     /gene="atpD"
                     /locus_tag="VYO70_001767"
     CDS             6507..7913
                     /gene="atpD"
                     /locus_tag="VYO70_001767"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1713959.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            7924..8343
                     /locus_tag="VYO70_001768"
     CDS             7924..8343
                     /locus_tag="VYO70_001768"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1713960.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
BASE COUNT         2591 a         1536 c         1814 g         2424 t
ORIGIN      
        1 atgtatagtg cattgaagtt aaaatagtaa actttgattt ctaagacatt attagaaatt
       61 agattaaatt ccttaatcga tttgcgcatg ttttatttca tttcattata ctttagattg
      121 gaaaataagg aatcacataa atcgcaagaa atagggaatc tgattcgtgt ttataaccgt
      181 tcaaaaaggg aagaggctga aagtgagcca cttaatcttt atgtcgaaga tgaaaagggc
      241 aatctccttg cgggtttgat agcagagact tttggaaatt ggctagaaat cgagtatttg
      301 tttgtaaaag aggaactgcg agggcaagga atcggttcaa aactattgca acaggcagaa
      361 agtgaagcta agaatcgaaa ttgttgtttt gcctttgtca atacttatca gttccaggca
      421 ccggactttt atcagaaaca tggctacaag gaagtttttt ctttgcaaga ctatctctac
      481 attaggcaaa gatattatta ccaaaagaat ctgtaaggtg agtatggagg tataatacaa
      541 agagaggctc gcacataagt atggaggtgg actgatgtat atgagtaact atcgataaat
      601 accagttata acctagaaat tgtgtctttc tagacgatat tgaggacaat ataatcgtag
      661 ctccaaaatt aggaatcaag gcttatcagg ttaagaaaag aagtgatgtt gttgatattt
      721 taaaacaatt tatttaaact aaaaactctc tatcttttta aattgacatt aatccgagat
      781 tcccctctcg gattttgtgt tgctttcatt aaatttacag gtaagacttg ctagcttcag
      841 taaatataca caagtttttc agaatagagt gaaatccgaa agaaaatgga aataatgaaa
      901 gctcttgtag gcataagaaa acacctctgt gttatacttg ttgttcaacc acaaacacaa
      961 gaaaggcaca gagatgcaaa accattatac tacaaaaggc gaacaaaggg aaatcaaata
     1021 gagaaattgc ctctctgctt ggaaaagctc ctcaaactat ccacactaaa atcaagcgta
     1081 ggacagtccg aaaatgtctt ggaaaagggc gcttcaaaga ggtttattct gccgactatg
     1141 ctcaacagtc ttatgaaaat aatcgcaagc actcggtcaa gagatcaagc gtgaccaagg
     1201 aactaaagga aaagattctc cactatcata accaaaaatt tttgcctgaa atgatggtta
     1261 tggctaaagg ggttaacgtg ggaatttcaa ccatttacta ttggattcat catggaaaat
     1321 tggggtaaag caagcaggat ctgctttacc ctagaaaagg aaaagcgctt aagaaacagg
     1381 ccagcaccaa ctttaaacct gctggtcaat ccatcgaaca gcggcctgaa gctatcaatc
     1441 ttcgtttgga gaatgggcat tatgagattg atacggttct acttacgaga gcgaaaaact
     1501 actgcttgct tgtcttgacg gatcgaaaga gtagacatca gatcatccga ttgattccaa
     1561 ataaaagtgc tgaggtggtc aatcaggctc taaaactcat cttaaaacaa cacaagattc
     1621 tttccatcac ggcagataat ggaacggaat tcaatcgctt gtttgatgtg ttttctgagg
     1681 agcacatcta ttatgcgcac ccctatgcct cttgggaaag gggaactaat gagaatcaca
     1741 acaggctcat tcgtagatgg ttacctaagg gaaccaagaa aatgactccc aaagaagtcg
     1801 cattcatcga aaagtggatt aacaactatc ctaaaaaatg cttggactac aagtcaccca
     1861 gagaagactt ctggatggct aacttgaact tgaaatttag caaaatggaa ataattttta
     1921 ttaaacgctt ccaatagcgg ttaaaagttg acaaatttaa attttaggac tacactaagt
     1981 aagtaaattt tattttaaag gagaatttgt tatgaattta acatttttag gcttatgtat
     2041 tgcctgtatg ggcgtatctg tcggtgaagg tttattgatg aatggactgt ttaaatcagt
     2101 agcacgccaa ccagatatgc tttctgagtt tcgtagtttg atgtttttag gtgttgcctt
     2161 tattgaagga actttctttg taactcttgt cttctcattt attatcaaat aaatacatgg
     2221 aacgagaaga aaagggagga ttttagatgg aagaaagtat taatccaatc atctctattg
     2281 gtcctgttat cttcaatctg actatgttag ccatgacttt gttgattgtg ggagttattt
     2341 ttgtctttat ttattgggca agccgcaata tgaccttgaa acccaaagga aagcaaaatg
     2401 tacttgagta tgtctatgac tttgttattg gatttacaga acctaacatt ggttcgcgct
     2461 acatgaaaga ttactcactc tttttccttt gtttattcct tttcatggtg attgccaata
     2521 accttggctt aatgacaaag attcaaacga tcgatgggac taactggtgg agttcgccaa
     2581 ccgctaattt acagtatgac ttaaccttat cttttcttgt cattttgttg acacatatag
     2641 aaagcgttcg tcgtcgtgga tttaaaaaaa gtataaaatc ttttatgagt cctgtttttg
     2701 tcataccgat gaatatcttg gaagaattta caaacttctt atctttggct ttgcggattt
     2761 ttgggaatat ctttgcagga gaggtcatga cgagtttgtt acttcttctt tcccaccaag
     2821 ctatttattg gtatccagta gcctttggag ctaatttggc ttggactgca ttttctgtct
     2881 ttatttcctg catccaagct tatgttttta ctcttttgac atctgtgtat ttagggaata
     2941 agattaatat tgaagaggaa tagaaaggag taactaatgc acgtaacagt aggtgaatta
     3001 attggtaatt ttattttaat cactggctct tttattcttt tgctagtctt gattaaaaaa
     3061 tttgcatggt ctaatattac aggcattttc gaagaaagag ctgaaaaaat tgcttcagat
     3121 attgacagag ctgaagaagc ccgtcaaaaa gcagaagtat tggctcaaaa acgcgaagat
     3181 gaattggctg gtagccgtaa agaagctaag acaatcattg aaaatgcaaa ggaaacagct
     3241 gagcaaagta aggctaatat cttagcagat gctaaactag aagcaggaca cttaaaagaa
     3301 aaagccaatc aagaaattgc tcaaaataaa gtagaagctt tacagagtgt taagggtgag
     3361 gtcgcagatt tgaccatcag cttagctggt aaaatcatct cacaaaacct tgacagtcat
     3421 gcccataaag cactcattga tcagtatatc gatcagctag gagaagctta atggacaaga
     3481 aaacagtaaa ggtaattgaa aaatacagca tgccttttgt ccaattggta cttgaaaaag
     3541 gagaagaaga ccgtatcttt tcagacttga ctcaaatcaa gcaagttgtt gaaaaaacag
     3601 gtctgccttc ttttttaaaa caagtggcag tagacgagtc ggataaggaa aaaacaattg
     3661 cttttttcca agattctgtg tcgcctttat tacaaaactt tatccaggtt ctggcctaca
     3721 atcacagagc aaatcttttt tatgatgtgc ttgtagattg cttgaaccga cttgaaaaag
     3781 aaacaaatcg atttgaagtg acgattacgt ctgctcatcc tctaactgat gaacagaaga
     3841 ctcgtttgct ccctttgatt gagaaaaaaa tgtctctgaa agtaaggagt gtaaaagaac
     3901 aaatcgatga aagtctcatt ggtggttttg tcatttttgc caatcacaag acaattgatg
     3961 tgagtattaa acaacaactt aaagttgtta aagaaaattt gaaatagaaa gtggtgttct
     4021 tttggcaatt aacgcacaag aaatcagcgc tttaattaag caacaaattg aaaatttcaa
     4081 acccaatttt gatgtgactg aaacaggtgt tgtaacctat atcggggacg gtatcgcgcg
     4141 tgctcatggc cttgaaaatg tcatgagtgg agagttgttg aattttgaaa acggctctta
     4201 tggtatggct caaaacttgg agtcaacaga cgttggtatt atcatcctag gtgactttac
     4261 agatatccgt gaaggcgata caatccgccg tacagggaaa atcatggaag tccctgtagg
     4321 tgaaagtctg attggtcgtg ttgtggatcc gcttggtcgt ccagttgacg gtcttggaga
     4381 aatccacact gataaaactc gtccagtaga agcaccagct cctggtgtta tgcaacgtaa
     4441 gtctgtttca gaaccattgc aaactggttt gaaagctatt gacgcccttg taccgattgg
     4501 tcgtggtcaa cgtgagttga ttatcggtga ccgtcagaca gggaaaacaa ccattgcgat
     4561 tgatacaatc ttgaaccaaa aagatcaaga tatgatctgt atctacgtcg cgattggaca
     4621 aaaagaatca acagttcgta cgcaagtaga aacacttcgt cagtacggtg ccttggacta
     4681 cacaatcgtt gtgacagcct ctgcttcaca accatctcca ttgctcttcc tagctcctta
     4741 tactggggtt gctatggcgg aagaatttat gtatcaaggt aagcatgttt tgattgtata
     4801 cgatgatcta tcaaaacaag cggtagctta tcgtgaacta tcgctcttgc ttcgtcgtcc
     4861 tccaggtcgt gaagccttcc caggggatgt tttctatctc cacagccgtt tgcttgagcg
     4921 ctcagctaaa gtttctgatg aacttggtgg tggatcaatt acagccctac catttatcga
     4981 gacacaagca ggagatatct cagcctatat cgcaaccaac gtgatttcta tcactgatgg
     5041 acaaatcttc cttggcgatg gcctcttcaa tgcaggtatt cgtccagcca tcgatgcggg
     5101 ttcatctgta tctcgtgtag gtggttctgc acaaatcaaa gccatgaaga aggttgctgg
     5161 tacacttcgt atcgaccttg cttcataccg tgagttagaa gcctttacta agtttggttc
     5221 tgacttggac gcagcaacac aggctaagtt gaaccgtgga cgtcgtaccg ttgaggtctt
     5281 gaaacaacct gttcacaaac cattacctgt tgagaaacaa gtaaccattc tttatgcttt
     5341 gacacatggt ttcttggata ctgttccagt agatgatatt gttcgtttcg aggaagagtt
     5401 ccatgccttc tttgatgctc aacatccaga gattttggaa accattcgtg atacaaaaga
     5461 cttgccagaa gaagcagtct tggatgctgc gattacagag tttctcaatc aatctagctt
     5521 ccaataagaa tagaggtgtc agatggcagt gtctctaaat gatattaaaa caaaaatcgc
     5581 ctcaacaaaa aatacgagtc aaatcactaa tgccatgcaa atggtatcgg ctgctaagct
     5641 aggtcgctct gaagaagctg ctcgcaactt ccaagtttac gctcagaaag tgcgtaaact
     5701 tttgacagat atccttcatg gtaatggagc tggtgcttca actaatccga tgttgattag
     5761 tcgttctgtg aagaagacag gctatatcgt tatcacttca gaccgcggtt tggttggagg
     5821 ttataattcc tctattttga aagctgttat ggagttgaaa gaagaatacc acccagacgg
     5881 taaaggtttt gaaatgatct gtatcggtgg gatgggagct gatttcttta aggctcgcgg
     5941 tattcaacca ctttatgaat tacgtggctt ggcagaccaa cctagctttg atcaagttcg
     6001 taagattatt tcaaaaactg ttgaaatgta ccaaaatgaa ctctttgatg agctttatgt
     6061 ttgctacaac caccatgtca atacgctaac cagtcaaatg cgtgtggaac aaatgcttcc
     6121 gattgttgac ttggatccaa atgaagcgga tgaagagtac agcttgactt ttgaattgga
     6181 aaccagccga gaagaaattc tggagcagtt gttgcctcag tttgcagaaa gtatgattta
     6241 cggtgccatt atcgatgcca agacagctga gaatgctgcg ggtatgacag ccatgcaaac
     6301 agcgacagat aatgctaaga aagtcatcaa tgatttgaca attcagtata accgtgccag
     6361 acaggcggcg attacacaag aaattacaga aatcgtagca ggtgctagtg ccttagaata
     6421 ggctctagtc cagctcgtat gaaaatgaac ttaggaccta gttgagctag gaaccgacag
     6481 tatcttatat agaataggag aaggagatga gttcaggtaa aattgctcag gttatcggtc
     6541 ccgttgtaga cgttttgttt gcagcagggg aaaaacttcc tgagattaac aatgcacttg
     6601 tcgtctacaa aaatgacgaa agaaaaacaa aaatcgtcct tgaagtagcc ttggagttag
     6661 gagatggtat ggttcgtact atcgccatgg aatcaacaga tgggttgact cgtggaatgg
     6721 aagtattgga cacaggtcgt ccaatctctg taccagtagg taaagaaact ttgggacgtg
     6781 tcttcaacgt tttgggagat accattgatt tggaagctcc ttttacagaa gacgcagagc
     6841 gtcagccaat tcataaaaaa gctccaactt ttgatgagtt gtctacctct tctgaaatcc
     6901 ttgaaacagg gatcaaggtt attgaccttc ttgcccctta ccttaaaggt ggtaaagttg
     6961 gacttttcgg tggtgccgga gttggtaaaa ccgtcttaat ccaagaattg attcacaaca
     7021 ttgcccaaga gcacggtggt atttcagtat ttactggtgt tggggaacgt actcgtgagg
     7081 ggaatgacct ttactgggaa atgaaagaat caggcgttat cgagaaaaca gccatggtct
     7141 ttggtcagat gaatgagcca ccaggagcac gtatgcgtgt tgcccttact ggtttgacaa
     7201 tcgctgaata cttccgtgat gtggaaggcc aagacgtgct tctctttatc gataatatct
     7261 tccgtttcac tcaggctggt tcagaagtat ctgccctttt gggtcgtatg ccatcagccg
     7321 ttggttacca accaacactt gctacggaaa tgggtcaatt gcaagaacgt atcacatcaa
     7381 ccaagaaggg ttctgtaacc tctatccagg ctatctatgt gccagcggat gactatactg
     7441 acccagcgcc agcaacagcc ttcgctcact tggattcaac aacaaacttg gaacgtaaat
     7501 tggtacaatt gggtatctac ccagccgttg acccacttgc ttcaagctca cgtgccttgg
     7561 cacctgaaat cgttggagaa gagcactatg cagttgctgc tgaagtaaaa cgtgtccttc
     7621 aacgttacca tgaattgcaa gatatcattg ctatccttgg tatggatgag ctttctgatg
     7681 aagaaaagac cttggttgct cgcgcccgtc gtatccagtt cttcttgtca caaaacttca
     7741 acgttgcgga acaatttact ggtcagccag gttcttatgt tccagttgct gaaactgtac
     7801 gtggctttaa ggaaatcctt gatggtaaat acgaccactt gccagaagat gccttccgtg
     7861 gtgtaggttc tatcgaagat gtgattgcaa aagctgaaaa aatgggattt taagaggtga
     7921 cctatggctc agttaactgt ccagatcgtg acaccagatg gtctcgtcta tgatcaccat
     7981 gccagctatg tatcggttcg aactctggat ggtgagatgg ggatcttgcc acgacatgaa
     8041 aatatgattg cggttttagc agttgatgaa gtaaaggtaa aacgtatcga tgataaagat
     8101 cacgtgaact ggattgcagt aaacggtggc gttattgaaa ttgccaatga tatgatcaca
     8161 atcgtcgctg actctgcaga acgtgctcgt gatatcgata ttagccgtgc agaacgtgcc
     8221 aaacttcgtg cagaacgtgc aattgaagaa gcacaagaca aacatttgat tgaccaagaa
     8281 cgtcgtgcta agattgcttt gcaacgtgcc attaaccgta ttaatgtcgg aaatagacta
     8341 taagaaaaaa tgaacttgaa aatac
//
LOCUS       DAVLYV010000082         8270 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLYV010000082 DAVLYV010000000
VERSION     DAVLYV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.082"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..298)
                     /locus_tag="VYO70_001769"
     CDS             complement(<1..298)
                     /locus_tag="VYO70_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713961.1"
                     /translation="MIELKIGKPSFGFLPYESHIEGILTGNNLNKIDYYLSMLDMEAI
                     CLQYGNKRIYDFFIGRNIQCNNIYREYSSRIDLSSEEALIPFLVDALAKGSFCYL"
     gene            528..1342
                     /locus_tag="VYO70_001770"
                     /pseudo
     CDS             528..1342
                     /locus_tag="VYO70_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
     gene            1701..2654
                     /locus_tag="VYO70_001771"
     CDS             1701..2654
                     /locus_tag="VYO70_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1713962.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASSNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
     gene            2975..3109
                     /locus_tag="VYO70_001772"
                     /pseudo
     CDS             2975..3109
                     /locus_tag="VYO70_001772"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(3347..4766)
                     /locus_tag="VYO70_001773"
                     /pseudo
     CDS             complement(3347..4766)
                     /locus_tag="VYO70_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5022..6872)
                     /locus_tag="VYO70_001774"
                     /pseudo
     CDS             complement(5022..6872)
                     /locus_tag="VYO70_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(6859..7224)
                     /locus_tag="VYO70_001775"
     CDS             complement(6859..7224)
                     /locus_tag="VYO70_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1713963.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIDKSHCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSY"
     gene            complement(7234..7593)
                     /locus_tag="VYO70_001776"
     CDS             complement(7234..7593)
                     /locus_tag="VYO70_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713964.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSSFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
BASE COUNT         2616 a         1583 c         1380 g         2691 t
ORIGIN      
        1 ataaataaca aaaagaacct ttagctaacg catcaaccaa aaaagggatt aaagcttcct
       61 cactagataa atctatacgt gacgaatact ctctgtatat attattacat tgtatatttc
      121 ttccaatgaa aaaatcataa attctcttat taccatattg aagacatatc gcttccatat
      181 ccagcatgga taaataataa tcaattttat ttaggttatt tcctgtaaga attccttcaa
      241 tatgtgattc atagggcaaa aagccaaagc ttggttttcc aatctttaat tcaatcattt
      301 ttataaatct cctaatataa atttactttc atcgaactaa actcaaattg acataatttc
      361 tgtcatcatt cctgctttaa ttcacaacta ttcttatatt atgcaaaaac ttatacctga
      421 ttaacgcaat aatataaatt tagagactaa ccaagatgaa ttcgtccttt ttaacaattt
      481 attcagtaga cttgttcggt aactgtaaaa agtgttatac tatttttatg gaaacagtat
      541 acgacaaagc acaaaaactt aactcaaaaa acttcaaact attgattggt gtcaaaaagg
      601 aaacctttca actcatgcta gaacacctga attcagccta tcagattcag caccgaaaag
      661 gtggacgtcc acgtagtctg cccatggaag accagctcat tatgaccctc cgttacttgc
      721 gatattatcc cactcagcgt ctgctggcct ttgattttgg cgtcggtgta gctacggtaa
      781 atgccatcat cacttgggtg gaggatacac ttcgtgcgtc aggtagcttt gatttggacc
      841 atttagaagc cccgagtgct gctgtggcta ttgacgtgac cgaaagtccg attcagcgtc
      901 caaaaaaaac caaagcaaaa attattctgg taaaaagaaa cgacacacct taaaaactca
      961 aattatgctg gatttgacga cacataaagt ctgtcaaatg gccttttctg acggacatac
     1021 gcatgatttt actctcttca aagaaagtat tggacaaagt ttgcctgaaa cgacgcttgc
     1081 ctttgttgac ctaggttatt taggcatctt gaaatttcat gagaatactt tcattcctgc
     1141 taaaaattcc aaaaatcgcc gcctgagtga ggatgataag cagttaaata aagagatgtc
     1201 agcgatacga attgaaattg aacattttaa cgctaaattc aagaccttcc aaatcatgtc
     1261 agtcccttat cgtaaccgca gaaaacgttt cgagttacgg gcggaattaa tttgtgccat
     1321 catcaattat gaagtgaact agattctgaa caagtctagt ataattttac cacaaagtaa
     1381 catgatttgc aagcgatttc aataataaag atatatgatt atcctcagga ataaataaat
     1441 ttgaaagttc tataatgaag ttagccacct taccttagtc aagaattaga tgtttcacta
     1501 tgtttgagta agttgatgat ttcatttgat aacaggtttg aaactgttag gctaggtgcc
     1561 caaggctaat catagccttg gtttagctga aaaaacaggt tcaagggttc ctgttgtcaa
     1621 atgaaatgtg atttaaggta taagaaaaca cctctgtgct atacttgttg ttcaccacaa
     1681 acacaaggaa aggcacagag atgcaagaac attatacccc aaaagggaaa catttgacaa
     1741 tagataaccg tcgcttgatt gagcggtgga agaatgaaaa taagtccaat cgtgaaattg
     1801 caggcttgtt aggaaaggcg cctcaaacga ttcataatga agtcaaaaga ggtacaactt
     1861 tacaacaagt gagaaaaggg ctatacaaaa aggtctattc tgccgattac gcacaaactg
     1921 tttaccaatt caatcgaaaa cggtcggtga aaaagttaat tttaacaaag gaaatcagag
     1981 agaagatctt acactatcat aagcaaaaat tttcgcctga aatgatggtt aacaagaagc
     2041 aagtgaaagt tggtatttca accatctact actggtttca taatggtcat ttaggattga
     2101 cgaaggccga catgctttat cccagaaaaa ggaaaggtgt caagaagcaa gctagttcga
     2161 actttaagcc ggcaggtaaa tctatcgaag aacgtcctga cgttattaat cttcgcttgg
     2221 aaaatggtca ttatgaaatt gataccgtcc tactgactaa gataaaaaat tattgcctgt
     2281 tagtcttaac cgaccggcgg agcagacacc aaattataag gttaattcca aataaaactg
     2341 ctgaatctgt caatcaggcg cttacgttac tattagggga gcatcgtatt ctgtccatta
     2401 ctgcagataa tggttcggag ttcaaacgat tgtctgaggt atttcctgag gaacatatct
     2461 actacgcaca tgcttactct tcatgggaga gaggttcaaa tgaaaatcat aatcgattaa
     2521 ttcggagatg gttacctaaa ggaaccaaga aaacgactcc gaaagaagta gcttttatcg
     2581 aaaattggat taacaactac cctaaaaaat gcttggacta caagtcgcca agtgaatttc
     2641 ttttgggtgg ctaacttcaa cttgaaattt gggctttgtc tctatgctaa aggaatccgc
     2701 tataaataca agtactatag ccgtcggcat tcctcattaa cacgacccaa tttgattaat
     2761 cagtgttttc aaacaaaagg taaaaatgaa atctggctgg gggatttaac ttatattccg
     2821 acaaaagcag gtactcttta tttatcatta tatatatgtt tatagcagaa aaattgttgg
     2881 ttaggcaatg ggaaatcgaa tgcaagataa gttggtaacg aatgttttca atcaagctta
     2941 tcatagagaa aatctcaaat aagaactagt cggccatacc gatcaaggat ctcaatatac
     3001 tggagctaga tttcaagaac tccttaaaca gaaaagatgc aagtctagcc tgagtcgaaa
     3061 aggcaatcca tatgataatg cacccatgga atccttttac aaaacataaa aagagaactt
     3121 gtcaatgatg ccaatttcgc aactattgaa cgaaatcttt aaatatattg aaatctatta
     3181 caatcctaaa tatctccatt cggcattagg gtaccaataa ctaatagagt ttgaaaaaaa
     3241 tagtcactaa tcatcgagtc cactgaaaaa gtaagctcta cagttcaact aaaatgaaaa
     3301 aagagggatc tatctcctgc ttggatagat tcctctttgc ttttccttat ttttggttta
     3361 ttagagttat aatcctcttc atattaacca caaatatggt tgtggcggct tgtaactcca
     3421 tgtttaaaag acctggtgct ctagctacat taagaccatg tctgttttca agttccgcat
     3481 ttttggcttc tatcttgtag cgatgtttgg ccttttcttt aaaatagggc gcttcctgaa
     3541 acctcttctg gaaaatatgt tcatcgctct taaggggtat caaataagtc ttagacttag
     3601 ccccttcttt ataacagccc atatttgaca gtgcttgatg tcaaaatagt gggtttcctg
     3661 agccgtcctt cttcctgtct tttccttctt tagtgccata tgcccttctg gacaaacaaa
     3721 gagacctgcg tccttgttat agaaaaactc atcttccttc ctacgaaagc cttttgaaat
     3781 agttggatat ggcctagcga ctcaatgcac acgttctttc ttggtaaatt gcatattatc
     3841 cttcccagag taggccctat ctccaacaat agcttcaatc gtcacgccat tatccttaga
     3901 tttgtgatag agttcttcta aaacaggacc atcacttttt tcacccgatg tcaccacaca
     3961 ggcagtaata atccgttcat cggtcatggc aaaatgctct ttgtacccgt aaaaggaact
     4021 atcggctgac ttatgtccaa ttcttgcttc ttctttgaca gaggattcca agtgctctaa
     4081 gtcatcctct acagcttctt taaggtaatt gaattttgtg agatagctgg tattccagtc
     4141 aattcgtcat gcttctcaag gactgatatt aattcttgtg tatatcttaa ttcagctact
     4201 agagtatctt cctgaggctt actaggaaat tctgccttga tatattccga atactgataa
     4261 atcgttttac gcaaggcttt tgagcgttct cggaggactt cttgaggttt cttgtgattg
     4321 taatgaactt tggtatgagt cgcatccaca atgagaatgt tacttttaat cagaccgagc
     4381 tctatcgcaa tctgaacaga tttagcgatg agcaaatcaa gtaatcgttc atctttgttc
     4441 cgaagttttc ggaatttagt caatgaggac ggttcaataa caggagctag accaaggaag
     4501 tatttgaagg tcataccaga aaaggagcgc tccaccacat ccacatcgga caacttatag
     4561 atgtctttta ataagagata cttgaacatc atgataagga gagagtagga acgacatccg
     4621 aagtcaggtt gataattctt ttccagctcg tcataaataa aactaaaatc acagagttcc
     4681 attagctgtc gtaaaaaatg agttttggga acaacaatat cacacagagt agaataggga
     4741 ctaacatcga ttatcaagca taccatcacc tcatctttag tataccatca tacgatgact
     4801 ttttcagtgg actccgaatt atcttaactc ttgacaagtc cagtgatgca atgatagagc
     4861 atagacaact ttaatgattt ggggtcgaag cgtaatcgta aaataggtaa aatcgtcttt
     4921 gaatgcattt ttattaatgt ttgttttaga agttaaaccg atattggcaa ccataaacag
     4981 aaaagatatc caaaaaaatt tgagtttgtc ttcagtctga tttatatttc attcataatt
     5041 tctttattga tacactcatc aaaaacctta aataattgtc caagctttct tattgttttc
     5101 tcatactcat caagaacatt accaaattcc tgttgtaact tcaaaagttc ttcattgact
     5161 tgttctaaag ttgttgattc tgtcatgttc aatttagaaa attcatgcct agctagtttt
     5221 tggctacccg aaccttcact cttagaattg ataagtactt ccgccatctt atttaaggtg
     5281 gcaatttttt catttgtttg agtcagtttt atatctagct ctgctatttc ggaaaccaag
     5341 ttgtctttaa tgtcttggta tttcttatct gtctcagtta actcaatcaa gaggctaatc
     5401 tcagaaatct tttcttgaag tctttcaaca gtcggtcttc tgtatggtat tactcgacta
     5461 tcgttggtta gctgcctaat caaggttctc cctttgatat actgattctt atccgaatgt
     5521 tctttgttat agacaaaata tgaggttgtc tcacgaatgt agactttata ttttttctga
     5581 ttttcttctt ccataatatc cagttgatag ttaggaatga aaataagacc actctgattt
     5641 ataccaaagg acactttgat ataaatcccc tcatcaacta acttctcaat ttggtgttct
     5701 gtaagtttta cttcaaattc atgaacggca tctcgttttt ccttaaatgt ctcaaaggct
     5761 tcctcaattt cttcagtaga taccttctcc ttatctcgtt cttcttgaaa agcacggtac
     5821 tgtgcctgta gattttctat tccttctgaa acacctactt ccttattttt aaaataatct
     5881 tgaaaaaaat tgacatcata taatttctca tcacttattt ttttatgact cagactgaat
     5941 tgctttccat tttcttcaag tataaagatt acattttttt gttttaagtc aatggttaga
     6001 ttcaattctt ttgattttgt tagtaaccct tctaaagaat tgacacagtt caacaaaaat
     6061 tctaaacgac ttgcaatctc ttgcttggca aaatgcattc taaaaaaatc ttcatcatat
     6121 aaatctcgtt tactgagttg acgtcctcga attggttttg tcattgcccg atccgtcatc
     6181 atgaatcgac tatgcttctg actaaaatca atctgaacat gcaactgctt tgctttctct
     6241 aaaaaatcat caaactattt agattgctgc atcaaaaaat aaagacgttg tttcaattca
     6301 aacttatgac tagattccct atatttttta taacctcgat aagaataacg tttctcaata
     6361 atttttgcac cagccatttt agaaatacgg tctgaaatca tacggaggtt tcgttccaag
     6421 gcatagttcc atatcaactt tttatctgaa ttgcggtcaa tagcgttgat taggatgtga
     6481 ttatgaacat gatctttgtc tgtgtgagtt gctacgataa acttaaaacg acctcctgtc
     6541 aattccatca tggtctcata accaatgcgg ttaatttctt caggtgtcag attatcttca
     6601 ggagaaaatg attggatgag atgatgggca tgaatagttt gttggtgttt ttcttgtcgg
     6661 tcatttctgg actcgtacaa cttgtcgtta ttagtaaagt taacattgta catttctacc
     6721 atttctgtat ggctaggaaa atctaagtaa ttactcatgc caaaatcaga tactaatttt
     6781 agattatctg ttttatctgg attgaggata tatttaatca aacgcctacg atattttttt
     6841 ccgtgcgtcg caaagtgttt agtaactacc atgaaactcc ttcagtcttt tcacctccac
     6901 ttgaaattct ttgtccactt ccttaattaa ctctccgact cctttactca attcttgtaa
     6961 ctgttcctga gaaatcagac ggctttgatt gatagcacgc gcaatttgat taatgttatt
     7021 tccgatccgt ctcaattcaa atactaactg gtcataggta tccgtattaa tggtgacaaa
     7081 tgacatattg ggattcagca acactttcct ggcatagact gaaaaatttt gacagtgact
     7141 cttatcgatt cgctcattca attgatgtaa ttctgaatct gttagaaata cttttttgag
     7201 attggtacga taacgataaa ccatagtagc ctcctatctc atgtattttt cacgaaaccc
     7261 acgactgagt gactgtactt gattgacttc cgcaatcaat tcttgaacgc aggtcaataa
     7321 gattgacacg tgttcttgag tcacttggtg attctctctt gcgataatca aaaccaaatg
     7381 cacatctcga ctgatctgtt caaacttttg agactgccaa agggaaaacc attctttggc
     7441 agctaactgt ttgtcatcag tcatcaaaat tttatccctg acgaatgatg aaaagtgacg
     7501 ataattatca acctccatca ttttttcaat ctgttttagc tcatctgcgg ttagatttac
     7561 ctcttttcga atgtcacggt actgttcaga catctctttt cctcactttc atcctttctc
     7621 ggataaaccc cgaccccgtg tgtcatacgg gaaatttctt aaagtccttg ttcggtcttt
     7681 gcgagctcag gcattgtggt cacaataccc aaaaaatcat atcgccagct aaccaaaacc
     7741 ttccagtttt gacagctaac cataagataa cttggtggct tcgcccccaa acccccagtg
     7801 aaaaattgag gttttatttt cttaggaatg atattaggac aacagggatg atttggaaaa
     7861 ggtatcacat tattgaaagg aaggtaagaa aaaagagagc aaaagctctc ataacaatcc
     7921 atttaaatac tacagagtag ttaaaaatta gtaactaata ttttatataa taagaatctt
     7981 tctctgtctt attttttata acaatacctt ttttatcaaa tgtacctatc acgttttttc
     8041 gttatctaat atctgcattg taacttcatt caattcatat attgctccgt ctacaaaaac
     8101 tgtatcacca taaatttgta gtccggtcga ctgaattgcg ataccacagt ctattttact
     8161 gtaataacct ctaaaactat tatgaaaatg tggcttaaaa aaacaagcac ctaataaaac
     8221 aaatataatt accgttaaag gaaaaagtaa tacagcatga tcaggcttat
//
LOCUS       DAVLYV010000083         7908 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLYV010000083 DAVLYV010000000
VERSION     DAVLYV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.083"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(45..2591)
                     /locus_tag="VYO70_001777"
     CDS             complement(45..2591)
                     /locus_tag="VYO70_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1713965.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLAAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(2714..3154)
                     /locus_tag="VYO70_001778"
     CDS             complement(2714..3154)
                     /locus_tag="VYO70_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1713966.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQIHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(3129..3998)
                     /locus_tag="VYO70_001779"
     CDS             complement(3129..3998)
                     /locus_tag="VYO70_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1713967.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYCFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSLIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGINDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(3976..4476)
                     /locus_tag="VYO70_001780"
     CDS             complement(3976..4476)
                     /locus_tag="VYO70_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1713968.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKARGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            complement(4487..5185)
                     /locus_tag="VYO70_001781"
     CDS             complement(4487..5185)
                     /locus_tag="VYO70_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1713969.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(5178..5444)
                     /locus_tag="VYO70_001782"
     CDS             complement(5178..5444)
                     /locus_tag="VYO70_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1713970.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTACDVLATDWVEVHD"
     gene            complement(5512..6438)
                     /locus_tag="VYO70_001783"
     CDS             complement(5512..6438)
                     /locus_tag="VYO70_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1713971.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(6513..7328)
                     /locus_tag="VYO70_001784"
     CDS             complement(6513..7328)
                     /locus_tag="VYO70_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713972.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLVGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVVTILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(7332..7652)
                     /locus_tag="VYO70_001785"
     CDS             complement(7332..7652)
                     /locus_tag="VYO70_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1713973.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(7742..>7908)
                     /locus_tag="VYO70_001786"
     CDS             complement(7742..>7908)
                     /locus_tag="VYO70_001786"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1713974.1"
                     /translation="TDEVSSKHVFEVVDETDEVSSKHVFEVVDETDEVSNHTYGRATM
                     TWFEEIFEEY"
BASE COUNT         2341 a         1810 c         1390 g         2367 t
ORIGIN      
        1 attttacatg agatagctga gttttctttt tattttaggc ttatttatgc atttccgtat
       61 tgaagaacaa ctgcttcgac tgcagctttt tcacggctaa tcaagtcaac acgcgctgca
      121 atttccttga ttcccatacc gatgttacgg ctaagagcaa ggtcagaaag ttgcggttca
      181 aagaactcct tgtattccgc caagcgttgc tgagtcttaa atacatgagc aggaaggata
      241 acaaagctat caaagctcat atctcctcca agggctgcct taatccaagc ccagttttca
      301 cgcgcccaag accaagctgt tttctgagtt gcttgatgag ctaggaattg gtaataccaa
      361 gcagacaagt cctgtggttt gaccacaaat ttgtccttcc aagaagtaat caagttttgg
      421 atattatccg catctgtact gtatgcaaga gctgctgcca actggcgttt aaagacagca
      481 tctgttgcgt gagtataagt atcaagataa agtgctaaca agtctttagt ctcatgatgt
      541 ttcatctcat taatcagaac ttgtgaacga atagctgctg ggagtcctgc aagattctcc
      601 ttgtgtgttg cgaagatttg gctagcgact tgactagctt ctgcatcatt tgagcgaatc
      661 atcatcgaaa cagccagctg acgaaccaat tcatcctcat ctgattctcc gtctttagct
      721 tcaaaaccaa gacggtcata gttatgacga gccaatttag caaccagtcc tttgaaggct
      781 gtttcagcat ccgttccttc atcaataaag cgctcaaggg ctgaaatcac ttgagaaaca
      841 gctgaaacca ccagataaga ctcttcctta gcaagtttat caagaactgg aagcaagtct
      901 gcataagaaa tgtgccctgc ctcagccaac aaacgacgtt cttgaacaat ttgcagtttg
      961 cttgtgttat caagtgtctc tagctcagca agaacagctg ctaacaagtc tccttgatag
     1021 tcggtaatat agtgggcagt attttcagtg ttgagacgaa gagctccttc attttcagca
     1081 agaagagctg cgtagccagg gatttcgata ctttcagttt cgagtgtatc aggcaagcct
     1141 ttccagttgc tattgagggg caccacccag agacggttct tgtcttcgtt ctcaccgatg
     1201 aagaattgtt tttgtgaaat cttcaagaca tcattttcaa ctttgacagt aagaactggg
     1261 taaccaggct gttccaacca agaatccatg aaggctgcga catcacgtcc tgacgcttga
     1321 ccaagggcat cccaaagatc actaccaatg gtgttgctgt attggtgttt ttcaaagtag
     1381 gcatgcaaac ctttagcaaa atcagcatct cctagccaac ggcgaagcat gtgcatgaga
     1441 cggcttcctt tggcatagac gatagcgccg tcaaagagtg tattgatttc atctggatgt
     1501 ttaacttcga cgtggacaga ctgaacgcca tcagtagcgt cacgttcaag agcaagaggt
     1561 actccacctg tttggaaatc ttcaaagata ttccagcttg gttcgatggt atccacacag
     1621 acgtattcca tcatattagc gaaactttca ttgagccaaa ggtcatccca ccatttcata
     1681 gtcacgaggt tcccaaacca ttggtgagcc aattcatggg ccacaacaag ggcaacttgt
     1741 tgacggctag caaatgtaga gttctcatcg acaaccaagt aaacttcacg gtaagtcaca
     1801 agaccccagt tttccatagc accagctgag aagtcaggaa gggcgatgtg gagagattga
     1861 ggaattgggt acttaactcc atagtaatct tcgtaaaact cgatagagcg aacagcgata
     1921 tccagtgaga aatcaagatt tgaaagtgga tgtgctttgg ttgagtagac acctaccagg
     1981 gtaccatttt tagttttagc ggtcacccct tgcaaatcac cagcaacaaa ggccaacaag
     2041 taagaagaca tgcgaggtgt tgtctcaaac ttccagatac ctgtttcctt acggttttca
     2101 acatcgattt ctggcatgtt tgacaaggcc aattcacctt ctgcttggtc aaagcgaaga
     2161 gagaggtcaa aagttgcttt ggcttcaggc tcatccacac atgggaaagc ttcgcgcgca
     2221 aaatggctct cgaactgagt agacaagacc tccttcttga ctccatcaac tgtataataa
     2281 gaagggtaaa tccctgtcat gttgtctgta attttaccag aaaaggcaag aaccaattca
     2341 acttgaccag cctcagccaa ttcgatatga agggcttcat tgtcatggtc aactgtaaat
     2401 ggacgagctt gacctgcaac ttctacagag gtgatttcca agtctttttg gtggagggag
     2461 atgcggtcac tctgtgcttg accagtgatg gtcactttcc cagaaaaagt cttggtctca
     2521 cgactcaaat ctaaaaataa atcataatgt tcaggaacaa attgcttaat aaaatgttca
     2581 actgcttgca tagttttctc ctattctaag tttaagagct gctgctcttc ttcaaacaaa
     2641 cttattatat catattttgc ttgagaaaaa aggattggac agcgatttcc aaaactttct
     2701 tccttctaat agtctacagt gggtagacct tgcgaaattc ttctaaaaca accttgcttt
     2761 caggtgtaaa agtcagccct acttcaccca aacagtctgt gaaaaagtcc tcatgaatct
     2821 ggcgccaata ggcaagtgat ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa
     2881 cttgattgaa gagttcaaca gaaacctttg taatttcgac aatgcagaca gactgatttt
     2941 gactatctaa aatgacatcg aaggtcccta cttggggaag tggttcgtct tctagtacat
     3001 agaggtcata ggctgatgct gttgctgtct tttctccttt aaacaccaaa tccgctaaaa
     3061 ggtctggttc aactccaaaa gcccaagcat cgatttcatc tccgatcaaa ggattgattt
     3121 gcttgtattt attccacatt tcttgcggta tcatgggtac tcctttgtaa ttttttactt
     3181 tcttctttta tgtgtttaag atgatctgga tggtcaatct ctaaatcaaa aatctctgga
     3241 atagaactgt agtggataat gcacttgata cccaactgat tcattttttg tatgaaagaa
     3301 gtattcagat agcctgctac agcaaaatta atcttgttct ttcttgcttt atcctgcata
     3361 tctcttagca tatctaacat tattggactt tccagatcat gccattgact gtttctcata
     3421 gtcgcaaaaa caaaggaagt caaatcattt attccaacta caatctttga aatgcccgtt
     3481 tccagtatac tagataagtc aaaatacgct gacggtaatt caatcatcgt tccgactttc
     3541 ccagtaaagc catactgacg caatactgta atagcttgtt ttaattggtc ggcatcattg
     3601 acaaaaggaa agataagaga cagattgggg ttggtttgat aaacttctgt aacgacatgt
     3661 gcttcagcct gaaattcatc caaacacgcc agtaaacgcc tggttcctct atagccaaac
     3721 aagggatgcc cttcgtcaaa aaactcttta gtccccacta aacaattggc ttctgtattc
     3781 gttaattcag taaaacaata ccaaacttct tcatctgagt acagggagca gatagtctct
     3841 agataatctt ttacaaattg ctgacaactt gctaatatga tgttttgatt aagctctctc
     3901 aacaaatatt ccccacgaat catgccgacg tggtgaaata gttgaggata aattttttca
     3961 agaatttttt cgccactaag ggcaagttga tttctcatca ttcaccttcc aattcatgta
     4021 agaagtcttg tccagttctg gaaatcctaa taattcagac ttaaccttca agactaatgg
     4081 cgatgcattt tcttctgtaa tctcttgaat atccatccaa atatatccaa gtgaatcatt
     4141 cgcaccatca gacacagctt ccgaagtcgt aacttgaggt gcactctcat tcatttcaac
     4201 atcatacaag gccatgacat ggtgaaccat aaaatttgtt aactcttctc tgacgaaaac
     4261 atcgtagatt cgaggattag agtagcttct aacagtaaat cccgtctctt ccataacttc
     4321 tctagtcagc gtttccgtca gtccttcacc aagttgctga ctgcctccag gtagatcata
     4381 ccgatgttga taagggcctc tcgctttttc aatgcagagt aacttcccat tttctaagca
     4441 aacagcgtag actccaaagt gttttttgat ttccatccaa ctcctcctac ttcaaagacc
     4501 agccaccatc tattgtcaag atttgtcctt gcatggcgct cgcttttcca cttgctaaaa
     4561 aaagactaag ctctgctatt tcctctggct caatccagcg cttgattggg gtttcactag
     4621 ccacccagtc agccaaacca cctggttcaa aatccgcagc ggtcatagct gtcttgactg
     4681 ctcctggagc gatcccaaag acctgaatcc cagcttcagc atagtctaga gccaactgct
     4741 tggtgaagcc agccaaggca tgcttggatg aagtataggc gtgaccacct ccacctgcta
     4801 ggctagaagc aatggaacac atattgatga tgattccctt tttattttcc agcatttgtg
     4861 tcaaataata ccgagtcaac tctactggaa taatgtagtt gatttcaaaa atctcttgaa
     4921 tgtcctgcgc cgtttgttcc aacagtggtt tgtaatcatc caaaactccg gcagtattac
     4981 acaaaacatc cacctgaggg caccagtcaa aaataggttc caagtccaag gtcaaatctc
     5041 tctgtaaaaa gtgaaaatca ccctctaaga gtggcttttc accttggtca actccataaa
     5101 cttgatagcc cttctctaaa aagaggcgag cttgagccaa tccgatccct gaactcactc
     5161 ctgtaatgag tacacgttta gtcatgcact tctacccaat ccgttgccaa aacatcacaa
     5221 gctgtcgggc tccacatgga aaaaccttct ccttctccag acacgttgat taggaaataa
     5281 ggtgtcattt caagtgcaag cccattttgc tcgatggtat caaagagttg gacatagttt
     5341 tccgcacctc cccaaccagt tcgtacatat tttctcttag cctttaaccc aggcaggatc
     5401 tcttcaaatg tcatgttttt ctcctttaat tctacattct tcatttaatt ataacaaaaa
     5461 aaccgcttta aaacggcttt ttgactgtga tttatttaca tcttcttcta cttacggcaa
     5521 attattccct gcagcaagat aaatttcata ccattctttt cttgttaagc taaagtttgc
     5581 cgcttggcta acttctctca agtgcttagg atttgtggta cctacaactg cctgcatttt
     5641 tgctggataa cgcaatatcc aagaaatggc aatagttgaa gaggttactc catatttaat
     5701 agctaaacga tcaagtactt gatttaaagc ttgaaatttc tcatttccaa caaaattccc
     5761 tttaaaatac ccgaattgta agacagacca tgcttgaatg accacatcgt gtaatttgca
     5821 atattcaaaa atgctgccat ctcgcatagc tgcttgacta tcttccatat taacatgaaa
     5881 agctgattca aatcctggag taaaagccgc actcaattgt agctgattaa cagctaacgg
     5941 ctgcttgaca tcttttttaa gcaactccat catcatagga ttttgattag aaactccaaa
     6001 atctcgaact ttaccttgtt tataaaggag atcaaaggct tctgctactt ggtcagattc
     6061 catcaaagca tctggtcgat gaaggagcaa gctatctaga tgatcaatct tcaatctttg
     6121 caaaataccg tctactgatt ttataatata gtccttagaa aaatcaaaat aggtaaattc
     6181 ttcaatgcga atgccacatt tggactgaat ccacatcttt tctcttaaat ctggacgatt
     6241 ttttaggaca agacctaaca gttcttcaca acgaccacga ccataaatat cagccaagtc
     6301 gaaggcattg attccaacag aaagtgctgt ttctacaagc tcttcaactt cttttacaga
     6361 cttatctttt attctcatca ttccgagaac aatttctgat aattctttgt catcttgacc
     6421 aagagttatg tatctcatca aatttttctc ctttaatttc taacattctt cccttcatta
     6481 taacaaaaaa ccgctttgca acggcttttt gactatactt cactccattt tatcttctta
     6541 aacccacgga acaagacaaa gattccaata aagaggacag ctaaaggaat aacttttgta
     6601 aggaaaacat ttgaaattcc catccactca taataacgga gcagagaacc caccacaaga
     6661 tgggcaataa tcatactgac aaatggacga aagaccgctt ctttccaatt ccaaataccg
     6721 ataactagcg aaatcgtaaa gacagacaaa ctatcccagg gagccggaat ataaaaggct
     6781 ccttcttgta tgaagcttgc cattcctaca tatcctaaaa caactagaag aactatagtc
     6841 ccaacaacaa tgtaagtgcc aattttcatt ttaggagaat cttggactaa acttcttcgt
     6901 aaaattgtga ccacaagtcc aaatccaatc agaaaaataa gaagttgccc taaaaatgtg
     6961 agcaaattga ctgttaagag aggaccttta gaaaaatcac ttagtagttg ataataacgt
     7021 aataccgcca ggacaagaat tggcgtcaaa agggactctt tgatagaact gcgaggtgct
     7081 cccttgagaa tctctttcat tattttttta ggattcttac ctagataatc ctctgcactc
     7141 atgccatctc gttctgcttc tgagaaatct agcatcatca aatagatctg ctctctgaga
     7201 tagtcttcat catagagaaa tccaacaaga ttaaaacttt cccacaactc ctcaaaatac
     7261 ttttgattct cctcagaaaa ctcatgtagc aaagcgcttg tttcttcgta atacttcatt
     7321 ttcttcatgg tttaaccccc attcttaatc ccttctactt tttgactcaa atcgtcccat
     7381 tgttgccaaa agactgagac acgctcttct ccttctttca ttaatgaaaa atacttccga
     7441 tctggaccat ctggcgacgg gcgcatgtcg cctcttatcc attgattttt ttctaacttt
     7501 tgcaacaaag gataaatagt tcctggaacg atagtatcaa atccagcctc tcgcaaagtc
     7561 tgaaccaact cataaccata ccgctctttt tgaccaatca tatccaagac acaaccttca
     7621 agaacacctt ttaatagctg agtttctttc atcacttctc ccttctaatc tattttgtaa
     7681 tacctactag taacttcacc tatagtatat cacttctaca ctagtttgta aagcataata
     7741 gttaatactc ttcgaaaatc tcttcaaacc acgtcatcgt cgccctaccg tatgtatggt
     7801 tactgacttc gtcagtttca tctacaacct caaaaacatg ttttgagctg acttcgtcag
     7861 tttcatctac aacctcaaaa acatgttttg agctgacttc gtcagttt
//
LOCUS       DAVLYV010000084         7886 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLYV010000084 DAVLYV010000000
VERSION     DAVLYV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7886
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.084"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            75..1586
                     /locus_tag="VYO70_001787"
     CDS             75..1586
                     /locus_tag="VYO70_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1713975.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            1608..2939
                     /locus_tag="VYO70_001788"
     CDS             1608..2939
                     /locus_tag="VYO70_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1713976.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPGKANYQG
                     PLYEQVCNDLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            3034..3161
                     /locus_tag="VYO70_001789"
                     /pseudo
     CDS             3034..3161
                     /locus_tag="VYO70_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836165.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fucose isomerase"
     gene            complement(3344..3631)
                     /locus_tag="VYO70_001790"
                     /pseudo
     CDS             complement(3344..3631)
                     /locus_tag="VYO70_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3619..3870)
                     /locus_tag="VYO70_001791"
     CDS             complement(3619..3870)
                     /locus_tag="VYO70_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1713977.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQATE
                     TSSALISSHYLDEQDLSEKLKSELQWLELENKLLNLWEH"
     gene            complement(4269..5153)
                     /locus_tag="VYO70_001792"
     CDS             complement(4269..5153)
                     /locus_tag="VYO70_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1713978.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(5298..6449)
                     /locus_tag="VYO70_001793"
     CDS             complement(5298..6449)
                     /locus_tag="VYO70_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1713979.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(6595..>7886)
                     /locus_tag="VYO70_001794"
     CDS             complement(6595..>7886)
                     /locus_tag="VYO70_001794"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1713980.1"
                     /translation="YARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRN
                     ESVDMTEFTRRMDRGIYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFV
                     IKMFMIGRDLMVGNPRLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNT
                     QFDWNGIRKPFVFATENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTL
                     EGRAAAGFLHLINSGSCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANR
                     EYFRGGGFSTRFLTKGDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTD
                     PGWPTTWFAPRLTGKGAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMH
                     NVPEEDIFRPKNWSLFGTEDLESADYRACQLLGPLHK"
BASE COUNT         2221 a         1699 c         1511 g         2455 t
ORIGIN      
        1 agcgttttct tgaatgttta tttaagaaag tagttggttt tttacacttt gttagacatc
       61 aggaggaaaa acaaatgagt gaaaaagcta aaaaagggtt taagatgcct tcatcttaca
      121 ccgtattatt gataatcatt gctattatgg cagtgctaac ttggtttatc cctgcggggg
      181 cctttataga aggtatttac gagactcagc ctcaaaatcc acaagggatt tgggatgtcc
      241 tcatggcacc gattcgggct atgctaggta ctcatccaga ggaaggttcg ctcattaaag
      301 aaacgagcgc agcgattgat gtagccttct tcatccttat ggttggtggt ttccttggca
      361 ttgtcaacaa aactggtgct cttgacgtag ggattgcctc tatcgtgaag aagtataagg
      421 gccgcgaaaa aatgttaatt ttggtactga tgcctttgtt tgccctcggt ggtacaactt
      481 atggtatggg tgaagaaaca atggccttct atccactcct tgtgccagtt atgatggccg
      541 ttggttttga tagcctgact ggtgttgcaa ttattttgct cggttctcaa atcggctgtt
      601 tggcatctac tctgaatcca tttgcgacag gtattgcttc agcgactgcg ggagttggta
      661 caggggacgg tatcgtactt cgtctgatct tctgggttac cttgactgct cttagtactt
      721 ggtttgttta ccgttatgcg gataagattc aaaaagatcc gactaagtca ctggtttata
      781 gtactcgcaa agaagatttg aaacacttta acgtagaaga atcttcatct gtagaatcta
      841 cacttagcag caaacaaaaa tcagttctct tcttatttgt gttgacattc atcttgatgg
      901 tattgagctt cattccatgg acagaccttg gcgttaccat ttttgatgac tttaatgctt
      961 ggttgactgg tcttccagtt attggtaata ttgtcggttc atctacttct gcactaggta
     1021 cttggtactt cccagaaggc gcaatgctct ttgcctttat gggtatcctg attggtgtta
     1081 tttatggtct taaagaagat aagattatct cttccttcat gaatggtgct gctgacttgc
     1141 tcagtgttgc cttgatcgta gcgattgctc gtggtattca agttatcatg aacgacggta
     1201 tgattaccga tacaatcctc aactggggta aagaaggctt gagcggtcta tcttcacaag
     1261 tctttatcgt tgtaacttat atcttctatc tacctatgtc attcttgatc ccatcttcat
     1321 ctggtcttgc cagcgcaact atgggtatca tggctccact tggagaattt gtaaatgtcc
     1381 gtcctagctt gattatcact gcttaccaat ctgcttcagg tgtcttgaac ttgattgcac
     1441 caacatctgg tattgtgatg ggagctcttg cacttggacg tatcaacatt ggtacttggt
     1501 ggaaattcat gggcaaactc gtagtcgcta ttattgtagt gaccatcgcc cttcttctcc
     1561 ttggaacctt ccttccattc ctataaaata gtgagtgagg tgattccatg aaaatagata
     1621 taacaaatca agttaaagat gaatttctta tatcattaaa aaccttgatt tcctatcctt
     1681 cagtactcaa tgaaggagaa aatggaacac cttttggaca agcaatccaa gatgtcctag
     1741 aaaaaacttt agagatttgt cgagacatag gtttcactac ctatcttgac cctaaaggtt
     1801 attacggata tgcagaaatc ggtcagggag cagagcttct ggccattctc tgtcatttgg
     1861 atgttgttcc atcaggtgat gaagcagatt ggcagacacc gccatttgaa gcaactatca
     1921 aagacggctg ggtattcgga cgtggtgtcc aagatgataa aggcccttcg ctcgcagctc
     1981 tctatgcagt aaaaagcttg ctggaccaag gtattcagtt caaaaagcgc gtacgcttta
     2041 tctttggtac cgatgaggaa accctctggc gctgcatggc acgctacaat accatcgaag
     2101 aacaggccag tatgggcttt gcacctgact catcttttcc tctgacctat gctgaaaaag
     2161 ggcttctaca ggtcaaactt catggccctg gatcggatca actagagctt gaagtaggag
     2221 gcgcctttaa cgttgtacca ggcaaggcca actaccaagg tcccctctat gaacaggttt
     2281 gtaacgatct caaagaagct ggttatgatt accaatccac tgaacaaacc gtaacggttc
     2341 tcggagtgcc aaagcatgct aaggatgcta gtcaaggtat caatgctgtc atccgactag
     2401 ctaccattct tgctcctctc caagaacacc ctgctctcag ctttcttgca acacaagcag
     2461 gtcaagacgg cacaggaaga caaatctttg gtgatatagc agatgaacct tctggtcacc
     2521 tatcctttaa tgtcgcaggt ctcatgatca atcatgaacg ttctgaaatc cgtattgaca
     2581 ttcggactcc tgtcttagct gacaaggaag aactagtaga gttgcttaca agatgtgcac
     2641 aaaactacca actccgctac gaagagtttg actatctagc gcctctatac gtcgcaaaag
     2701 acagtaaact cgttagcaca ctgatgcaaa tctaccaaga aaagactggc gataacagtc
     2761 ctgctatttc atccggtggt gccacttttg ctcgcaccat gccaaattgt gtagccttcg
     2821 gcgccttatt cccaggagcg aagcagacag aacatcaggc aaatgaatgt gccgttctag
     2881 aagatttgta ccgtgctatg gatatttatg ccgaagccgt ctatcgactt gcaacttaat
     2941 caggcaactg tttctaccaa aaaaaatcga ccgattaatg aactgcaccc caaaagttag
     3001 acagaataaa tctaactttt ggggtgtttt attatgaaat tgagttatga agataaagtt
     3061 cagatctatg aactaagaaa gcaaggacaa agcttcaaac agctttcaaa aagatttgtg
     3121 tggatgtttc tggtctaaag tcatctgaat ctttgagatg agctttataa atcgcttttt
     3181 tcagtttttg cactggtgtt tcgataaact caaacttttt agccgtggta ttgcctgatt
     3241 ttatagtata ttgaaactag aatagtacac ctctacttct aaaacatttt tagaaatcga
     3301 tttgactgtc ctgaacgatt tgtcctgttc ttatttcatt ttactatatt tgagccactt
     3361 cgtctttaac ggctttattc ataagctctt gtaatttttc tttactatca attacttctg
     3421 attttccgtt gtaatttatt gtaataggtt ttaacttacc taatttctcg acacgctcat
     3481 taatttgatc ttttttgaag gctgcatatg tttttcctaa gattttttcg aaaatatatt
     3541 tatcagatag cggtttgtct tcttcagctt ggtttttgta ttaatttgaa acgtaaggaa
     3601 caaatccttc atagtaacct aatgctccca taagttcaaa agcttgtttt ctaattcaag
     3661 ccattgcaac tcagatttca gcttttcaga taaatcctgc tcatccaaat aatgacttga
     3721 aattagtgct gaactcgttt ctgtagcctg tacaggctga gcacccatac cagcaaaaaa
     3781 taaactcgtt cctagcaaga ccgaacaagc tcctattgca tatttcctca aagaaaaacg
     3841 ctgctttctc tcaaattgaa attctttcat cccatctccc atcattcatt attactgtat
     3901 attttgtata tcagaaatag tttgtattca caaatctttc tagttattcc cttatcattc
     3961 ctaattaagg gagataacat acaataattt ttagttaaat gtatatcgat gttttttgtt
     4021 tttcttaata aacgcaatac aaaaagagcc tgttgccaag ctctttgtac tcaatgaaaa
     4081 tcaaatagca aactaggaaa ctagccacag gttgctcaaa acaccgtttt gaggtagtag
     4141 ataagactga cgaagtcagc tcaaaacact gttttgaggt tgcagataga actgacttcg
     4201 tcagtaacat ctatacggca aggcgacgct gacgtggttt gaagagattt tcgaagagta
     4261 ttagtctatt atttcttctc agcgcgaagg gctgacaaga tttgtgttcg gatatcatcc
     4321 acaccatttg gagtatttgg taaaaagatg gtttgatttc ctttagaggc aaaggtattc
     4381 aaggtatcca aatactggtt ggtcaagagg atagacatga tttgttcttc tgtcatgcca
     4441 acattggctt ccttgagttc ggtgatagac tctgccaatc catccacaat cgccttacgt
     4501 tgttgggcaa tccccacacc atgaaggcgg tctttttctg cttctgcttc agctgcagtg
     4561 acaattttaa tcttgtcagc ttccgccaat tcttgtgctg cgacccgctt acgttgcgcc
     4621 gcattgattt cattcatgga ttgcttaact tctgcatccg gttcgacttt ggtaatcaag
     4681 gttttcacga taatgtagcc gtaagtggtc atttcttctg ctacttggtg ttgaacttca
     4741 agggcaatct catctttttt ctcaaacaat tcatccaagg ttaattttgg aacagaagag
     4801 cgaagagcat cttcgatata agatttaatc tgagattctg gacgtatgag tttatagtaa
     4861 gcatctgtca cgctctgctc gttgacacgg tactgagtcg ctacattcat cataacgaac
     4921 acattgtcct tggtcttagt ctcaaccaca atatcacttt gcaacaagcg caactgaatc
     4981 cgtgctgcaa tcgagtcaat cccaaaaggc aagcgaatat gaataccgct attagcaacc
     5041 ttttggtatt tcccaaagcg ttcaataatc gccaccgact gctgacgaac cacataaact
     5101 gtactcagtg tgactatcac caataggagc acacaaacaa tcagaaaaat catgaaaaat
     5161 attgccataa tggaacctcc acaagtattt ttctagtatt atagcacatt taaagaaggc
     5221 tgtgccgttt ttactgcgat ttttcctgaa atgtcaataa ttagaggtga attgtcctat
     5281 tgtcgtccaa tctcttgcta aaataactct ttataaaagg caatcgtttc ttctaaggtt
     5341 ggcataaatg gatttcctgg tgcgcaggca tcaatcaagg cattcttaga aaggtattca
     5401 aagtcgaaat ctttttcttc aataccaagt tcagtcagtt tcttaggaat acctactgtc
     5461 tcagaaagct tctcaatctc aacaatcgca taatcggcac attcttgatc tgatttacct
     5521 tctacatgaa gtcccaaggc tttggcaaca ttgcggaaag cttctggtac acgtttagca
     5581 ttttcacgtt ctataactgg tagcaacatg gcacagcaaa caccatgtgg caaattatat
     5641 actgcaccga gttggtgagc cattgagtga acataaccaa gaccagcatt attaaagctc
     5701 ataccaccaa ggaagatggc attgaccata ccttcacgcg cttcaatatc atgtccattt
     5761 tctacagcac gaggaagata ttccttgata agctcaatag caccaataga cagtttcttg
     5821 gttacatcat aagcaccagg tgttaccaaa gcttcaacag cgtgagtcag agcatccata
     5881 ccagtagcag cagtcaggcc tttaggtttg gaaagcatga gttccgggtc attaacagag
     5941 ataagagcaa ggctattctt gtcaaccatt accatcttaa ccttgcgttc ttcatcagta
     6001 atcacatagt taatagtgat ttctgcagaa gttcctgccg ttgtattgat agccacaact
     6061 ggcaagcctt ttttagcaga cttatgaagc ccttcataat cctgtggttt tccaccattt
     6121 gtagccatga tagaaataca actagctgca tcctgtggag aacccccgcc aagactgatg
     6181 ataaagtcac agccatgttc ttgcaaagca gctaccccat ctgtgacatt tttacaagta
     6241 gggtttggct ctacatcgct aaagatgaca tattcgattc cttctgtatc cagtggtttt
     6301 aaaaccttag gtaaaatgtc actgccttcg atgtacttat ctgtcaccaa aagtgccttt
     6361 ttatagccaa gttccttgat ataaggaccc acttcattta caacaccttt accaataagg
     6421 ttgactgccg gaacataaaa tgtagccata taattttcct cctgcgcctc cgcgctatct
     6481 atttaacatc attatattag tttttgtatt cgttttcaat tatttgtgaa ttgtttaaca
     6541 aatcttttta aaaggatagg agggacataa gttcacctcc tatatacaca atttttattt
     6601 atgtagcggt cccaacaact gacatgcacg atagtctgct gattctagat cttctgttcc
     6661 aaataaggac caatttttag gtctaaagat atcttcctcg ggtacattat gcatattgac
     6721 aggaattctc aacatagaag ccaaggtaat caagtctgct ccaatgtgtc catatgttat
     6781 ggctccgtga ttagctcccc aattattcat gacgtcatag acagacttga aagcaccttt
     6841 tcctgtcaaa cgtggagcaa accaagtagt tggccatcct ggatctgtac gattatctaa
     6901 agtatggtga acatcttcag gaagttcaag tgtataacct tctgcaattt gtagcactgg
     6961 accaacccct tttagaagat tgagacgtac cattgttact ggcatatccc ccttcgtcaa
     7021 gaaacgagtt gagaatcctc ctccacggaa gtattcgcgg tttgctggtg ggaagtctgt
     7081 attttcaagc atagcctgca cttcactttc ttccaactcc cagaatggtt tcataacagg
     7141 tttgccatct cgagtagctt gacctgtacc atccaatgta caagaaccag agttgattag
     7201 atgtaagaag ccagctgcag cacgaccctc taaagtatgt cccgttacac gtttaacagc
     7261 ctctggactc caataagtac gcacatcagc aaatatttgt ggagtatttg ttaatagata
     7321 attaaagagc atagacacac catttagtga atcattctct gtcgcaaata caaatggttt
     7381 tcgaatacca ttccagtcaa actgagtatt gaggaaagtt tccataaaat ctccatttgg
     7441 aaaatggtct gtccactgac gttgaccttg gaaaccagct actaaagcat ggtgaccaac
     7501 tgcttcttcc tcaaaaccaa gttcagcaag tcttgggtta ccaaccatta agtcacgtcc
     7561 aatcatgaac atcttaataa caaattccca ttgtctatct ttttcttcac ggcttaaaac
     7621 aaggtcttca cggttatggt cgaatccttc ttttacgttt tctttcaccc atttgagcgc
     7681 acgttcaaac tcttctgggt cataaatacc acggtccata cggcgcgtga actccgtcat
     7741 atctaccgat tcatttcgca ttcctaagta ttcttggaag aaatctggat ttacaataga
     7801 accgccaatc cccatcgaaa cactacccat tgataggtaa gcagtgtctc tcatcaagcc
     7861 agttgcaaga gctgcacgcg cataac
//
LOCUS       DAVLYV010000085         7775 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLYV010000085 DAVLYV010000000
VERSION     DAVLYV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7775
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.085"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(51..3893)
                     /gene="spuA"
                     /locus_tag="VYO70_001795"
     CDS             complement(51..3893)
                     /gene="spuA"
                     /locus_tag="VYO70_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1713981.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATSPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDETGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLREEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRKESVENSSKENISATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            4310..4723
                     /gene="rpsL"
                     /locus_tag="VYO70_001796"
     CDS             4310..4723
                     /gene="rpsL"
                     /locus_tag="VYO70_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1713982.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            4743..5213
                     /gene="rpsG"
                     /locus_tag="VYO70_001797"
     CDS             4743..5213
                     /gene="rpsG"
                     /locus_tag="VYO70_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1713983.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            5638..7719
                     /gene="fusA"
                     /locus_tag="VYO70_001798"
     CDS             5638..7719
                     /gene="fusA"
                     /locus_tag="VYO70_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1713984.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     SKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
BASE COUNT         2129 a         1561 c         1737 g         2348 t
ORIGIN      
        1 taatcccccg agctataatg cttgggggat ttttctatag gagggctagc ttagttctct
       61 tttttatttt ttagtaagaa accgagacca aggagcgcaa ggaggctgat tcctgcaaat
      121 aggagtttgt tttcgttttt gattcctgta tttggaagtt cagcttgtct atctggggtt
      181 gcagatatat tttccttgct agagttttct accgattctt ttcgaaccgc ttcttttaca
      241 gatgatgctt gtgcttcttg tgctggttgt tcagcttgtg aatcagctgt agcttggcta
      301 ggtttatttt ccgcatcagc tactttggca tctggtttag tagaatcagg tctagcttct
      361 ggagctgggt cttgatgtgc agggtgagaa gcttcatttt gatgttcagg cttggaaggg
      421 gttgagtctg gtttctcttc tgcagttgac tcatggctag ttccattttg agagactcga
      481 agaacagtag ctgtaagggc attcaatttc aagccttttt cagtccattc aagtcctttc
      541 gggttggcaa ttccgactga tcctgcttgg ttttcatctg ccaaaacttc cgcatttctt
      601 agatgtgcaa aggcagttcc caaattaaat tcgcgagctt tttcatccgc attgacaaag
      661 actgcgtaga tatcgccgtt tggagcagtg atttggtagc caatcactac atcctctttt
      721 tccacaccat tttggcctgg gacagtgatg aggtggacac ggtctttgat atcttggaga
      781 ctcttaagtc ggaaggcatc tgtagattga cgaagggcaa tcaaaccttt catatagtca
      841 cggctcttga cattttcagg ataagcttta ccatctgtag ccttagtcca gtcaaacttg
      901 ttgactgcgt cgctagaatc gtaagaatca tggatgaagt aaggatagtc aaatgggttg
      961 ccgtccttat cacgcaacaa gtgagatttg tttggaacct tatcctctgc tactggagtc
     1021 ttgtaggctg ggtcacggaa ttgtttagta cgtccatatt cctgaccgga gtggataaat
     1081 ggagttcctt gagctgtcaa gaccatgaga tttccaagtc gtaaacgacg gtggatttca
     1141 gcatagttct cagccttgct tgggtctttt ttgatagact gggcaatgat gtcaaagagg
     1201 gtcaagttat catgggctgc gatgtattgg atgacatctc cagggctgtc agcttcaaag
     1261 ttagttggtt gagcaatgag atttttaaag atggtgttga catcacgctt gccacctgtg
     1321 ataaaggcag gttgaccttc gtttggataa ccagatttga ggttgttacg gatgtcatct
     1381 gaaaagacag cgacagtatc ggtatgtttc atccaatctt ggtcagcagc tttagtaggc
     1441 atgttttcat caccggcata ggttctccaa ccttcgccta acatgattag atttggattg
     1501 agggcgcgtg cagccttgta agcttcttcg atagaagcgg cgtcatggtc tcccatcata
     1561 tcgaagcgga agccatccac tttgtaggta tcaactaggt atttgataga gtcaactagg
     1621 agccgtttgg tcatatggtg ggttgtcccc aagcgtccac caccaaagct agttcgaggt
     1681 gtgccatcgg catccataaa gtggtagtag tttggctcta ggtcttcaaa gatatcgact
     1741 ttggctgtgt ggttataaac gacatctagg atagctccca taccacgttt gtggatttcg
     1801 ttgatgaggt ttttaaattc tgcgattcgt ttttctggat tcttaggatc gcttgagtac
     1861 ataccagtca aggagaagta gttttgaggg tcatatcccc agttgtagtt gctgttgctt
     1921 gaagcatagt cagacaagcg ttcatggttt ttcaattcat tgacaaagta gtaagacaag
     1981 actggaagga gctggatatg ggttacaccc aagtctttga gatagtctag tttttcaatg
     2041 aaggcttcaa aagtcccaaa tggtttggtc aagtcttttg caatggcagg atctgaagtg
     2101 aagtcacgca catgagcttc gtagataacg gcgtcttcac gagtcttgaa attgtgaatc
     2161 ttaccataag tcaagtcttg aggtccgagt ttagctggat ctacaaaggc ggctttagcc
     2221 actttatggg catcgtcaat cttggcatcg tcgctattcc aagcagcaag agatttagcg
     2281 taaggatcga gtgcaagaac agttttacct tgacgctcga tttggtattg ataatagtag
     2341 ccagtgaaat ctgtgattcc gagtttgttt gtgctgtcta gagtttgttt ccaagttcct
     2401 ctttcccctt tttcgagagc gacagttcca actactttgt cagggtcatt cttgtcgtag
     2461 acaacaacag aaaccttatc agcacttggt gaccaaaggg tcaaatcaac ttgttttcct
     2521 tcttctctta ggtcagctcc cagtttgcca tcatagctgt atgtctcatc tttcaggcgc
     2581 cagcttgttt tggtagtgaa ttgatcggaa ttgtagctaa cagtataagg atgttttgtg
     2641 tcagagaaat ctccgctgta ggtcactttc ttaccagttt catcgattgc aacatcggta
     2701 atagttacct tgtttcctag gtgattagtg atgttggagt gtttgaggat atcttctttt
     2761 ttagcaccga caagtgttga aaagctactt tcaatgctag aagtgcctac gtgttgggct
     2821 cctgtcatac ggatatcatg gacatagtat ggatttgtgt aaatcgattc atcatcgtct
     2881 tttaggaaaa tttggctatg atttttcaaa tctgtgaact tataattttc tttacggatt
     2941 ttcacgtcgt ctccttgttt gctctcatct agtaataaaa atccaaattc tcttgcggct
     3001 tcattaagag gaatgtcgat atagcggcca tatttgcctg tagccgtaaa gtctgttccg
     3061 tcaggccatt gagcgctact tggatttttt acatctcccc agtaccagag agatttcttg
     3121 tcatagttgc catctgtgcg gtagtagttg acgcgaacag ttcctgcagg ctgtggctcg
     3181 taagagaaaa ccttgtaatc ttggtctaac caagcttcgt tcatttttgg aactagtttt
     3241 tctacagatt tatcgccggt tagatttttt ccagctgtat tgttgatgag gaagctaatt
     3301 ttcttggctt gttctccctt taatttgaca tctaggtaat agccgtagtc atctttcttg
     3361 gcatccttga aggacaaagc tccgtttggc cagttttcag atggtttttc aacatcgtcc
     3421 caagtccata gtccttgagc atccttgttt tcttcaggaa gttttttgac atggatacgg
     3481 aagtagttat cttcgatggg ctcttctgcc ttagtttcag ttgttactgg actagtagaa
     3541 gtagttggct cacttgaacg atcttgtcca gtttgtggtg ctggatcggc tgggcttgta
     3601 gcaggttttt cttctgtttt ctctagggaa gcgtttgcat ttctaggtga agaaatatca
     3661 ctttcgttct taccagagcc agctgctgta tcagtaagag gttgagcaag ggtggttgta
     3721 tttctgttgt tgggttcgtt agtggttggt gtactttcgt cggatgagat agttggtgta
     3781 gccatggcaa gcaagacaag gcttgcgccg ataaggacag aaccagttcc atttttgagg
     3841 gaacggatgc tgtagaccat ttttttctca gtgtgagatg gtgtttttct cataatgatt
     3901 ctccaatcaa taaatttcta tatcagtata gcatgtagta aaacaatatg caagcgtttt
     3961 ctttgatttg tgaaaaaaat tcctactttt gagaagtttt ctgtactatt ttatatatca
     4021 gtataaatga cggagataaa aatgtcacat aagccaagcc gtattattag gaagatgatg
     4081 tgttcccagc tatttttatt cagaaattga tctctaacat taccaataaa aatgaaaaat
     4141 atcttgacaa gcaagggaaa attttgttac aataatagac ggtacttttt acttttggtc
     4201 tctcaagagt gtacagggac gtgctgacaa atgttgcaaa agtacacaca gatgatatct
     4261 gtcaccaagt gtatcatcac caaaaataaa aaaacacagg agaatgtaga tgcctacaat
     4321 taaccaattg gttcgcaaac cgcgtaaatc aaaagtagaa aaatctaaat caccagcttt
     4381 gaacgttggt tacaatagtc ataaaaaagt tcaaacaaac gtttcttcac cacaaaaacg
     4441 tggtgttgca actcgtgttg gaacaatgac acctaaaaaa cctaactcag cccttcgtaa
     4501 attcgctcgt gtacgtttga gcaaccttat cgaagttact gcctacatcc caggtatcgg
     4561 acacaacttg caagagcaca gcgtggtgct tcttcgcggt ggacgtgtaa aagaccttcc
     4621 aggggtacgt taccatatcg tccgtggtgc acttgatact gcaggtgtta acgatcgtaa
     4681 acaaggccgt tctaaatacg gtactaaacg tccaaaagca taaggaaagg ggataaagag
     4741 aaatgagtcg taaaaataga gctccaaaac gtgacgtatt gccagatccg ctatacaatt
     4801 cacaactagt tactcgtctt atcaaccgcg ttatgcttga tggtaaacgt ggtactgctg
     4861 cttcaatcgt ttacggtgct tttgagcaaa tcaaagaagc tactggcaac gatgcacttg
     4921 aagtatttga aacagctatg gaaaacatca tgcctgtact tgaagtacgt gcacgtcgtg
     4981 ttggtggttc taactaccaa gtcccagttg aagttcgtcc agaacgtcgt acaacacttg
     5041 gacttcgttg gttggtaaca atcgctcgtc ttcgtggtga acacacaatg caagaccgtc
     5101 ttgcaaaaga aatcttggat gctgctaaca acactggtgc agcagttaag aaacgtgaag
     5161 atactcaccg tatggctgaa gctaaccgtg cattcgcaca cttccgttgg taagatagga
     5221 tgcgaaagcg ttaagaaagt cccagagaaa atagggaatc gaagcaggtt gcggttgcaa
     5281 ccaatgagat tcatcttttt ctccagactt ttagcttgag ctcaactaaa tcatgatgct
     5341 aggcacggta aggatgcaag gtgaaaatag gaaactgacg cagtattcaa cgaatacaag
     5401 gagttttatc tttttcacgc agcatcccgt tccagctcac atcggctaac taactttagc
     5461 ctgggttcaa attagctaaa tcgattagta ttagctataa ctcagcttac catctcgtaa
     5521 gttgaaacca acaatagcat gaaaacattg agaacgggta ggtcctgcct atccgttttt
     5581 attaaaatcg tgttataata gaatagaaat caaaaataaa taggagaaac aaacctcatg
     5641 gcacgcgaat tttcacttga aaaaactcgt aatatcggta tcatggctca cgtcgatgcc
     5701 ggtaaaacaa caactactga gcgtattctt tactacactg gtaaaatcca caaaatcggt
     5761 gaaactcacg aaggtgcgtc acaaatggac tggatggagc aagagcaaga acgtggtatc
     5821 acgatcacat ctgctgcgac aacagctcaa tggaacaacc accgcgtaaa catcatcgac
     5881 acaccaggac acgtggactt cacaatcgaa gtacaacgtt ctcttcgtgt attggatggt
     5941 gcggttaccg ttcttgactc acaatcaggt gttgaacctc aaactgaaac agtttggcgt
     6001 caagcaactg agtacggagt tccacgtatc gtatttgcca acaaaatgga caaaatcggt
     6061 gctgacttcc tttactctgt aagcacactt cacgatcgtc ttcaagcaaa tgcacaccca
     6121 atccaattgc caatcggttc tgaagatgac ttccgtggta tcattgactt gatcaagatg
     6181 aaagctgaaa tctatactaa cgaccttggt acggatatcc ttgaagaaga catcccagct
     6241 gaataccttg accaagctca agaataccgt gaaaaattga ttgaagcagt tgctgaaact
     6301 gacgaagaat tgatgatgaa atacctcgaa ggtgaagaaa tcactaacga agaattgaaa
     6361 gctggtatcc gtaaagcgac tatcaacgtt gaattcttcc cagtattgtg tggttcagcc
     6421 ttcaaaaaca aaggtgttca attgatgctt gatgcggtta tcgactacct tccaagtcca
     6481 cttgacatcc cagcaatcaa aggtattaac ccagatacag acgctgaaga aactcgtcca
     6541 gcatctgacg aagagccatt tgcagctctt gccttcaaga tcatgactga cccattcgta
     6601 ggtcgtttga cattcttccg tgtttactca ggtgttcttc aatcaggttc atacgtattg
     6661 aatacttcta aaggtaaacg tgaacgtatc ggacgtatcc ttcaaatgca cgctaacagc
     6721 cgtcaagaaa tcgacactgt ttactcaggt gatatcgctg ctgccgttgg tttgaaagat
     6781 actacaactg gtgactcatt gacagatgaa aaatctaaaa tcatccttga gtcaatcaac
     6841 gttccagaac cagttatcca attgatggtt gagccaaaat ctaaagctga ccaagacaag
     6901 atgggtatcg cccttcaaaa attggctgaa gaagatccaa cattccgcgt tgaaacaaac
     6961 gttgaaactg gtgaaacagt tatctcaggt atgggtgaac ttcaccttga cgtccttgtt
     7021 gatcgtatgc gtcgtgagtt caaagttgaa gcgaacgtag gtgctcctca agtatcttac
     7081 cgtgaaacat tccgcgcttc tactcaagca cgtggattct tcaaacgtca gtctggtggt
     7141 aaaggtcaat tcggtgatgt gtggattgaa tttactccaa acgaagaagg taaaggattc
     7201 gaattcgaaa acgcaatcgt cggtggtgtg gttcctcgtg aatttatccc agcggttgaa
     7261 aaaggtttgg tagaatctat ggctaacggt gttcttgcag gttacccaat ggttgacgtt
     7321 aaagctaagc tttatgatgg ttcatatcac gatgtcgact catctgaaac tgccttcaag
     7381 attgcggctt cactttccct taaagaagct gctaaatcag cacaaccagc tatccttgaa
     7441 ccaatgatgc ttgtaacaat cactgttcca gaagaaaacc ttggtgatgt tatgggtcac
     7501 gtaactgctc gtcgtggacg tgtagatggt atggaagcac acggtaacag ccaaatcgtt
     7561 cgtgcttacg ttccacttgc tgaaatgttc ggttacgcaa cagttcttcg ttctgcatct
     7621 caaggacgtg gtacattcat gatggtattt gaccactacg aagatgtacc taagtcagta
     7681 caagaagaaa ttattaagaa aaataaaggt gaagactaat ccgtcctcac tctagaagga
     7741 agtcacttag tggcttcctt ttgtctttag aaaat
//
LOCUS       DAVLYV010000086         7737 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLYV010000086 DAVLYV010000000
VERSION     DAVLYV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7737
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.086"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(41..730)
                     /locus_tag="VYO70_001799"
     CDS             complement(41..730)
                     /locus_tag="VYO70_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1713985.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVCLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(742..1620)
                     /locus_tag="VYO70_001800"
     CDS             complement(742..1620)
                     /locus_tag="VYO70_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1713986.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAATHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(1610..2479)
                     /gene="licA"
                     /locus_tag="VYO70_001801"
     CDS             complement(1610..2479)
                     /gene="licA"
                     /locus_tag="VYO70_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1713987.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKITPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     IKGLASYGGSDEK"
     gene            complement(2496..3518)
                     /locus_tag="VYO70_001802"
     CDS             complement(2496..3518)
                     /locus_tag="VYO70_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1713988.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(3523..4230)
                     /locus_tag="VYO70_001803"
     CDS             complement(3523..4230)
                     /locus_tag="VYO70_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1713989.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKNIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4566..6053
                     /gene="tacF"
                     /locus_tag="VYO70_001804"
     CDS             4566..6053
                     /gene="tacF"
                     /locus_tag="VYO70_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1713990.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            6063..6866
                     /locus_tag="VYO70_001805"
     CDS             6063..6866
                     /locus_tag="VYO70_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1713991.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            6868..7677
                     /locus_tag="VYO70_001806"
     CDS             6868..7677
                     /locus_tag="VYO70_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1713992.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2440 a         1540 c         1304 g         2453 t
ORIGIN      
        1 atcaaaaaac atccgactat tttgtcagat gttggaatct ttaattttcg tttttaagaa
       61 tttcttctaa tttacgatag tcttggacac tatcgatctc ataaatgcta ttgccttcta
      121 attcttcaac atagacatct agctctttga tattatcctt aaccatattg tcccaataga
      181 gatcaacaaa ttcaccactt gcataagcct tgtcgataaa gctgacaatc ttttctgcag
      241 ttggagcatc ccagaaggat acaccactaa ggatgcgacc tgccttgcta tcaacaataa
      301 tgtcttgaac cttgtagtca tctccataga ccaagaacca ttcgttggta caatcttcac
      361 gataaacact aaaataagtc gaacgagtca aatcattgcg gaacatattt ttaaagagat
      421 agttatctgc atcaataaca tagctgttgg ccaattcttc ttttacaaga tagagagagt
      481 aaaagttatt gtagtcagcg tatttatcat tgaaaacgag gcaaacaccg tatttttctt
      541 ttaaataatc gaattgttct ttaagataac caacgatgat gatgatgtca ttgattcctt
      601 tttctttgag aaactcaatt tggtattcaa tcaaaggttt ttgattaacc tgaaccaagg
      661 ctttaggggt attttcagtc ataggacgca agcgagttcc caatcccgct gctaagataa
      721 tggctttcac acgaatctcc tttattcttt aataataata taaactccag caatgacgac
      781 aagtgacgtc ataatggtca gcatatctaa tgatgttccc aagaaaagag ctgcgaacaa
      841 gacagtccat actacatagc tcacgttcaa gcctgtagcc ttggctggtt gcaagcgatt
      901 gatagcgata taataagcca agtaggaaat catatcaaag gctgcaaaaa caatcatgag
      961 acctagcaat tgtccattgg ctactgcagt aaatgactga tgagagaaga gcacaatcac
     1021 aagataggac aagaacgaag ttacttgacg gattaagagg gcttcgattt cactcaattc
     1081 actttccatg gcaaaagagc taagaacact ctcacttccc catgcaatag cacaaaccaa
     1141 agcacaaaga atcccaatgt agaaagaatt aacctgttca accttatagg tctgagcaat
     1201 aatccctcca ataatcaaga caatcccaaa tacagtattt ttcgaaatct tgtgcttcaa
     1261 aaagaagaaa gccaatagaa ctgaaatcgc agggtaaata gccgatacag atgaagctaa
     1321 agaacttccg atatacttaa ctgcataaag attggcctgc ataccgatag ggcctgctag
     1381 caaggctccg atgataacac tgacattgcg aatatttaag aaaattgaga ggcgaacttt
     1441 cccttctttt accaagagaa aagctagtaa gataaagatg ctcaaaaaat catgagttgc
     1501 agccaccaca aagggtgaca aatctgtaaa aatcgaaaag atataagcac taaccgttag
     1561 acccaagccc cagaaaatac ctgagagaag gccaaaagga actccgtttt tacttttcat
     1621 ctgaacctcc ataagaagcc aaacctttaa tagctctttg gtaacgattc acaccatagt
     1681 caccaaaatc ttcaccttgc tcttccttat agacagtcca tagactccaa atagtatctt
     1741 gtaaaatttt ataaatagca atcttttcat gagaaaccgg tgtttggtca ctctcatagt
     1801 gagataagaa agtttcttcc tcttgggaag tgaattcaga ctctaaaaag agggcagcca
     1861 aatcccacat tggatcattc attgatgaat attcccagtc aatcaaataa agtcgtcctt
     1921 gaggtgattc gataaagttt tcaggcacca aatcgatatg acaagatttt ctgtcaacac
     1981 ctaagtcagc cagtcttttc tctaaggaga agactgcatt tctaacagat tcatagttgg
     2041 cataaggaat ttgttcttca atcaaggatt cgtatttttt gatttcttca aaaggagcaa
     2101 attctcctct taattcctta gcagacgtat gaatagtttg taatattgga gtaattttgt
     2161 cgaacttggt cttgattgac gttgaatcaa gcgtaatcgc agattcgata tactcattta
     2221 ctttgatacc agcttcaata tcaaaaagat aattttttac atctaagcct aaatccttta
     2281 gtagttcaag attgtacttt tcatcttgtc gattgataag cttttctgtc cctttaccaa
     2341 agaatttaac aatgtattgc ttatttgttg ttttggccaa atagttttga ttggtcattc
     2401 cacccagttg ttcaacactg aggacttcct cttcttgact aagtaaggaa gaaatttttt
     2461 ctttaatgat tttctccaca attaacctcc agtacttata cttcccactt aaacactgtt
     2521 ttaaaggctg tgtttaaatc ggttgcaaag acacgatgaa tatctttaat ttctcttaca
     2581 ggttcttcta gataaaggat atttttaaga cgattggcaa atttcttgac ttccatcatt
     2641 tggatagcat tttcaaaatc aatgcgacca gaacgagatg acccaaccaa aatcaagccc
     2701 ttttctaagg catcgcgagt attgagattg actttatatt cgctaactcc catcatgaga
     2761 atcgttccct gaggacgaat gtagcgaatc aagtcattaa tagctggtcc agtaccatca
     2821 ccaccacaac attcaaaagc atggtcaaag gccaaatctt caggaatatt atccgtaata
     2881 tagcattctt tggcaaatga gaagagttcc aacttttccc aatgacgacc aataaccaca
     2941 atctctgctt ctggcaaagt atagttgata atattggcaa ccacaaaagc taaacttcca
     3001 tctccaataa cggcgatccg ctcccgcttg ctatgagcaa gagtcaatag acgattcata
     3061 gcgtgcatgc ccacactgac aaactctgta atggctgcaa ccgtatcttc aatagcatca
     3121 taagccacca cacgatcttt agggagagaa acaaactctc tcataaagcc atcaaatcca
     3181 ctagacaaga aatgggtccc tgtcatgtag ttttcataga attcttcatc actctgcata
     3241 ggagactgat tgggaatcat gacaactttt tgaccaacct cgtaggttcc ggtcgggtca
     3301 gaaatgacgg ttccacatga ctcgtgaatc attgccattg gaagcttttt attcaaaatc
     3361 ttgggatcac gttttccctg atagtaacgc tgatccgcat gacagacagc catgtagttg
     3421 ggacggataa ggatatgatt ctcttggtca atagcctctt cctgatattt gacattgata
     3481 aacttaggct tagttagttg ataaatttga ttaatcattt tactagtctt tctcaatcat
     3541 actttttgca atcttcaaat ctgttacggt tgtaatcttc agatttgagt attcaccttt
     3601 ggccaaagcc acatcttttc ctttgatcac aaagatttta catgcatctg tcaagatttc
     3661 cttctcttca tcagaaagag atccataaag gtccatgaag tccttgcaac ggaatgtttg
     3721 aggtgtttgt ccttgataaa ggtgagcacg atttggaata tctgtaataa attgaccatt
     3781 ggtactttca acgatagtat caaccgcttc taccactgtg tccactgcgt catgattttg
     3841 ggcaagttgg atattgtcct gaatcatgcg aagtgtaata aatggacgaa cagaatcgtg
     3901 ggtaacaacg atatcctctg gagtaagcgg acgataagca tcaatggctt caatgatgtt
     3961 cttaatactt gtattgcggt cagcaccacc ctttgtaatg atgatacgtt ccttataaag
     4021 aggaagatat ttatctacaa gatcttctgc atgagaaacc cagtctccat gaacaccaac
     4081 tacaattttt tcaatacttg gctccaagac aaatttttca attgtatgaa tcaaaatagg
     4141 tcgatcacct agctctaaaa attgttttgg caagttactg atccccatgc gtgtgccagt
     4201 tccaccggca agaattcctg cataaatcat ctattttctc ctttgtctta ctaaaaaaac
     4261 ttattctctc tattatacca caatctagtc ctatcataaa agaaatactg gtcctcccct
     4321 tctagaatag gagaattatg cagttctatt attcaaagaa actagtccat ttctagtaat
     4381 gactaggaat tctgtagcaa ttactaataa atggctgcga aattttagag tccttcagaa
     4441 taatatactt tccttaaaga aaacttaaaa cttaagattt gaaacttcaa agtgattact
     4501 tgacttactg cccttcattt cttattagct aactttatga tataatgaaa aacgaggtaa
     4561 attgaatgaa aagtataaaa ttaaatgctc tatcttacat gggaattcgt gtcttgaata
     4621 ttatttttcc catcctaact ggaacctatg tcgcgcgtgt cttggaccga actgactatg
     4681 gttacttcaa ctcagtcgac actattttgt catttttctt gccctttgca acttatggtg
     4741 tctataacta cggtttaagg gctatcagta atgtcaagga taacaaaaaa gatcttaaca
     4801 gaaccttttc tagtcttttt tatttgtgca tcgcttgtac gattttgacc actgctgtct
     4861 atatcctagc ctatcctctc ttctttactg ataatccaat cgtcaaaaag gtctaccttg
     4921 ttatggggat tcaactcatt gcccagattt tttcaatcga atgggtcaat gaagctctgg
     4981 aaaattacag ttttctcttt tacaaaactg ccttcatccg tatcctgatg ctggtctcta
     5041 ttttcttatt tgttaaaaat gaacacgata ttgttgtcta tacacttgtg atgagtttat
     5101 cgacgctgat taactacctg attagttatt tttggattaa aagagacatc aaacttgtta
     5161 aaattcacct aagtgatttt aaaccactct ttctccctct gacagccatg ttagtctttg
     5221 ccaatgccaa tatgctcttc acttttttag atcgcctctt cctcgttaaa acagggattg
     5281 atgtcaacgt tagttactat accatagctc agcgaattgt gaccgttata gctggggttg
     5341 taacaggtgc aattggagtg agtgtgcctc gtctcagtta ctatctgggg aaaggagaca
     5401 aagaagccta tgtttctctg gttaatagag gtagtcgaat ctttaacttc tttatcattc
     5461 cactgagttt tggactcatg gttttaggac caaatgccat cctactttac ggtagtgaaa
     5521 aatatatcgg aggcggcatc ttgacctctc tcttcgcttt tcgtacgatt atcctggcct
     5581 tagataccat tcttggttcc caaattctct ttaccaatgg ctatgaaaaa cgtatcacag
     5641 tctatacagt ctttgctggg ctactcaatt tgggcttgaa tagtctcctt tttttcaacc
     5701 atatcgtggc tcctgaatac tacttactga caactatgct atcagagact tctctacttg
     5761 ttttctatat cattttcatc catagaaaac aactcatcca cttgggacat atctttagct
     5821 atactgttcg atactctctc ttttcacttt cctttgtagc aatttatttc ctgattaatt
     5881 tcgtgtatcc tgtagatatg gtcattaatt tgccattttt gattaatact ggtttgattg
     5941 tcttgctatc agctatctct tatattagtc tacttgtctt cacaaaagat agcattttct
     6001 atgaattttt aaaccatgtc ctagccttaa aaaataaatt taaaaaatca taggagttta
     6061 aaatgaaaca actaaccgtt gaagatgcca aacaaattga attagaaatt ttggattata
     6121 ttgatactct ctgtaaaaag cacaatatca actatattat taactacggt actctgattg
     6181 gggcggttcg acatgagggc tttatccctt gggacgacga tattgatctg tccatgccta
     6241 gagaagacta ccaacgattt attaacattt ttcaaaagga aaaaagcaag tataagctcc
     6301 tatccttaga aactgataag aactacttta acaactttat caagataacc gacagtacga
     6361 ctaaaattat tgatactcga aatacaaaaa cctatgagtc tggtatcttt atcgatattt
     6421 tccctataga tcgctttgat gatcctaagg tcattgatac ttgttataaa ctggaaagct
     6481 tcaaactgct gtctttcagt aaacataaaa atattgtcta taaggatagc cttttaaaag
     6541 attggatacg aacagccttc tggttactcc ttcgaccggt ttctcctcgt tattttgcaa
     6601 ataaaatcga gaaagaaatt caaaaatata gtcgtgaaaa tgggcaatat atggctttta
     6661 tcccttcaaa atttaaggaa aaggaagtct tcccaagtgg tacctttgat aaaacaatcg
     6721 atttaccctt tgagaattta agccttcctg cacctgaaaa atttgatact attttgacac
     6781 aattttatgg agattatatg accctaccac cagaagaaaa acgcttctac agtcatgaat
     6841 ttcacgctta taaattggag gattaggatg caatatttag aaaaaaaaga aattaaagaa
     6901 attcaactag ccctgctgga ctatattgat gagacttgta agaaacatga tattccttat
     6961 tttctcagtt atggaaccat gcttggagcc atccgccaca aaggtatgat tccttgggat
     7021 gatgatattg atatttccct ttatcgtgag gattatgagc gtttactgaa gattattgaa
     7081 gaagaaaatc accctcgcta caaggttctt tcctacgata catcttcttg gtacttccat
     7141 aatttcgcat cgattttgga cacttctact gttatagaag accatgttaa gtacaagcgt
     7201 catgatacca gccttttcat cgatgtcttc ccaattgatc gatttacaga cttgagcatt
     7261 gtcgacaaga gctataagta tgtggctctt cgtcaactag cttatatcaa aaaatcacga
     7321 gcagttcacg gtgatagcaa actaaaagat tttcttagat tatgtagctg gtacgctctc
     7381 cgatttgtca atcctcgcta cttttacaag aaaattgatc aactagtcaa aaatgctgta
     7441 accaacactc ctcaatatga aggaggagtt gggatcggta aggaagggat gaaagaaatc
     7501 ttcccagttg atacctttaa agaactgatt ttaactgagt ttgagggccg tatgttgcct
     7561 gttcccaaaa aatatgacca atttttaacc cagatgtatg gcgattatat gacaccacca
     7621 tcaaaagaaa tgcaagagtg gtatagtcat agcattaaag cttatcgcaa aaactgattg
     7681 agggggatta tacaaactac taagatagag gttattcaaa aacataattt tagtaga
//
LOCUS       DAVLYV010000087         7589 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLYV010000087 DAVLYV010000000
VERSION     DAVLYV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.087"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            80..622
                     /locus_tag="VYO70_001807"
     CDS             80..622
                     /locus_tag="VYO70_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1713993.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQKA
                     VKKEENSPFAGLQGLFDGDE"
     gene            622..1251
                     /gene="nth"
                     /locus_tag="VYO70_001808"
     CDS             622..1251
                     /gene="nth"
                     /locus_tag="VYO70_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1713994.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            1462..1983
                     /gene="pyrR"
                     /locus_tag="VYO70_001809"
     CDS             1462..1983
                     /gene="pyrR"
                     /locus_tag="VYO70_001809"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1713995.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            2002..2925
                     /locus_tag="VYO70_001810"
     CDS             2002..2925
                     /locus_tag="VYO70_001810"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1713996.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            2975..4054
                     /gene="carA"
                     /locus_tag="VYO70_001811"
     CDS             2975..4054
                     /gene="carA"
                     /locus_tag="VYO70_001811"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1713997.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            4367..7543
                     /gene="carB"
                     /locus_tag="VYO70_001812"
     CDS             4367..7543
                     /gene="carB"
                     /locus_tag="VYO70_001812"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1713998.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
BASE COUNT         2216 a         1553 c         1718 g         2102 t
ORIGIN      
        1 ttttaattac aaagtttggt gtgctgattt gtagcctttt catggtataa tcaagagagt
       61 aaatctttat ggaggaagta tgaaattaaa tattcaagaa attcgtaagc agtctgaagg
      121 tttgaacttt gaacaaacgt tagatttagt tgatgacctg cgtgcacgta atcaagaaat
      181 tttagatgta aaagatatcc ttgcagttgg gaaagtacaa tatgaagacc gtatgtattt
      241 cttagattat caactatctt ataccattgt tcttgcttcg agtcgcagta tggagccagt
      301 tgagttagtt gaatcttatc cagtcacgga agttttcatg gaaggcgcaa ctaaccagct
      361 agatcaagaa gttttagatg atgacttggt cttgcccatc gaaaatgggg agcttgacct
      421 tgctgagagt gtatcagaca atatcctgct aaacattcct atcaaggtct tgacggctga
      481 agaagaagct ggtcaaggat ttatctcagg aaatgactgg caaatcatga cagaggaaga
      541 ataccaagct caaaaagcag taaagaaaga agaaaacagt ccttttgctg gcttacaagg
      601 actatttgac ggagatgaat aatggtcttg tcaaaaaaac gagcacgaaa ggtgctagaa
      661 gaaatcattg ccctcttccc agatgccaag cctagtcttg attttaccaa tcattttgaa
      721 ctcctggttg cggtcatgtt atcagcccag acgacagatg cagcggtaaa taaggccaca
      781 ccaggtctct ttgttgcctt tccaacaccc caagctatgt ctgtagcgac agagagtgag
      841 attgcttcac atatttctcg cttgggattg tatcggaata aagctaaatt ccttaaaaaa
      901 tgtgcccaac agttactaga cgattttgat ggtcaagtcc ctcagacacg tgaagaattg
      961 gagagtttgg caggtgttgg tcgcaagaca gccaatgttg tcatgagtgt aggatttgga
     1021 attccagcct ttgcagtgga tactcatgtg gagcgtattt gcaaacacca cgatattgtc
     1081 aaaaaatcag cgacgccact tgaggtggaa aagcgggtca tggatatctt gccgcctgag
     1141 cagtggttag ctgcccatca ggccatgatt tattttggaa gagccatttg ccatccaaaa
     1201 aatccagagt gtgaccagta cccacaatta tatgatttta gcaatttgta agatgctaca
     1261 ttgaaacttt ttgcttgatt ttaagcatgc tttgtgctat agtaaatgat aactaaatat
     1321 tcctttaaac ctgtcccgtg aggcaggcaa ggagcgcgat aaagtagaag ggtgaagtct
     1381 atactgtgtg cagtagggct cgcttggcta taaatttcaa gtctccttgt ttaaggagac
     1441 ttttcttttt ggaggtttgt catgaaaaca aaagaagttg tagacgaatt gaccgtcaaa
     1501 cgagcgatta cgcgtattac ttatgagatt atcgaacgca acaaagattt gaataagatc
     1561 gtcttggctg gtattaaaac tcgtggtgtc tttattgccc accgaatcca agaacgtttg
     1621 aagcagttag aaaatctttc agttcctgtt gtggaattgg atactaaacc tttccgtgat
     1681 gatgttaaaa gtggagaaga tacttctttg gtttctgtcg atgtgacaga ccgcgaagtt
     1741 atcttggtgg atgatgtgct ctatacaggt cgtaccatcc gtgctgctat tgataatatt
     1801 gtcggtcatg gtcgtcctgc gcgtgtgagt ttagcagttc tagtcgatcg tggacataga
     1861 gaattgccaa tccgtccaga ttacgttgga aaaaatatcc caaccagtcg ttctgaagaa
     1921 atcatcgtag agatgacaga acttgatgac caagacagag ttctgattac tgaagaagct
     1981 tagaaagtta aaggagtagc catgtcagaa aatcaacaag cattgaacca tgtggtgtcc
     2041 atggaagacc tcactgtcga tcaagtaatg aaattgatca agcgaggaat tgagtttaaa
     2101 aatggagccc agcttcccta tgaagaccat ccgattgttt ccaatctctt ctttgaggat
     2161 tctacacgga cacataagtc ctttgaagtc gcagagatta aacttggatt ggaacgactt
     2221 gactttgatg tgaagactag ctcggttaat aagggtgaga cactttatga caccattttg
     2281 actctgtctg ctttaggagt ggatgtctgt gtgattcgcc atccagaggt cgactactat
     2341 agagaattga ttgctagtcc aacgattacg acttccatca tcaatggtgg agatggttcg
     2401 ggccaacacc ctagccagag cttgcttgat ttgatgacca tttatgagga atttggccac
     2461 tttgagggtc ttaaagttgc tattgcaggt gacttggacc actcacgcgt tgccaaatcc
     2521 aatatgcaga ttttgaaacg cttgggagct gaactctttt tcgctggacc tgaggaatgg
     2581 agaagtcaag agtttgcaga ctatggacag tttgtaacca ttgatgaaat cattgatcag
     2641 gttgatgtca tgatgtttct ccgtgtgcaa cacgaacgcc atgatagtgg agctgtattt
     2701 tcaaaagaag actaccatgc ccaacatggc ttgactcaag aacgttacga tcgtttgaaa
     2761 gaaacagcaa tcctcatgca cccagctcca atcaatcgtg atgtagaaat agcagaccac
     2821 ttggttgaag caccaaaatc acggattgtc caacaaatga ccaatggtgt ctttgttcga
     2881 atggcaatct tagaatccgt actagcgagt agaaacgcca actaaaacac aagacgttaa
     2941 aagacgccag tgagcgtcca cgagaggtga aaatatgaca aaaagaattc tagtattaga
     3001 agatggcaca gtttttgaag gcaaggcctt cggagcagat attgatgtaa caggcgaaat
     3061 cgtctttaat acagggatga ccggctacca agaatccatt acagaccagt cttataatgg
     3121 acaaatcttg acctttactt atcctttggt aggaaattat ggtattaatc gtgatgatta
     3181 tgaatccatt attccaactt gtaagggagt cgttgttttt gaagaagcgc gtagagctag
     3241 caactggcgc aaccaaatga ccttggatga atttttgaaa gccaagaaaa ttccagggat
     3301 ttcaggaatt gatacgcgtg ctcttaccaa gattatccgt aagcatggta ctatgcgtgc
     3361 aaccttgacc catgttgggg acagtatgga ccatgtgacg gaccagctcc aagcaacagt
     3421 tttaccgaca gacaatatca aacaggtttc tactaaaacg tcttatccag ctccaggagt
     3481 tggtttgagc gtggtgctag tggactttgg tctcaagcac tcaatcctac gtgaactttc
     3541 taaacgcaac tgtaacgtga cagttgttcc ttattcgaca acggctgaag aaattctcca
     3601 tctcaatcct gacggagtta tgttgtcaaa tggtccaggt aacccagaag acgttccaca
     3661 agcactcgac atgattcgcg gtgtacaagg aaaaattcca atctttggta tttgtatggg
     3721 acaccaactc tttgcaatgg caaacggggc taagacctat aagatgaagt ttggccaccg
     3781 tggattcaac catgcggtac gtgaaatcgc aacaggacga gtagacttca ccagccagaa
     3841 ccatggttat gcagtcagcc gcgaggattt gccagagcac ttgattatta cccacgaaga
     3901 aatcaatgac aagtcagtgg aaggtgtgcg ccacagatac caacctgctt tctctgttca
     3961 ataccaccca gatgcagctc ctgggccaca cgacgctagc tacctatttg acgagtttat
     4021 cgagatgatg gaagttttta aacaatcaaa ctaagaacga gtaaaagctc gtcggagaat
     4081 ttatgtaact gaacacggac ggaaagctca gaatgtagag aaaccaactt ggattgtcaa
     4141 tccttccttg atttcctaaa attcaatcgc tttctattcg tcctttgtat cttatttaat
     4201 gtcgccctga gaggcgacga atagaaagga gaagggtgct ccgtatttgc tgaactgaat
     4261 acgggctacg gacggtgcta aaaagatagt tattcctaga actgacagtt cttcgtcata
     4321 actcctattt tagctttgtc cgcttgacac ccttaatatc ttatatatgc ctaaacgtac
     4381 tgatattcaa aaaattatgg tgattggttc tggtccgatt attattggtc aggctgctga
     4441 gtttgactac gctgggactc aggcttgctt gtcgttgaaa gaggaaggtt atgaggttgt
     4501 tttggttaac tcaaatcctg caaccatcat gacggacaag gaaattgctg acaaggttta
     4561 cattgaaccg attacacttg agtttgtgac acgtattctt cgtaaggaag gtccagatgc
     4621 cttgctacca acactcggtg gtcagacagg tctcaacatg gccatggaat tatctaaaaa
     4681 tggtatccta gacgagcttg gcgttgaact tctgggtacc aaattatctg ccattgacca
     4741 agcggaggac cgtgacctct ttaaacaatt gatggaagag cttgaacaac ccattccaga
     4801 atctgaaatt gttaacacag tagaagaagc agttgccttt gcagcgacaa ttggctaccc
     4861 agtcatcgtt cgtccagcct ttacccttgg tggtactggt ggtggtatgt gtgccaacga
     4921 gaaagaattg cgtgaaatca ctgaaaatgg tttgaaattg tcacctgtta cccaatgttt
     4981 gattgagcgt tcgattgccg gtttcaagga aattgaatac gaagtgatgc gtgactcagc
     5041 tgacaatgcc ttggttgttt gtaacatgga aaactttgac ccagttggga ttcacacagg
     5101 ggattccatt gtatttgccc ctgcgcaaac catgtcagac tatgaaaacc aaatgcttcg
     5161 tgatgcgagc ttgagcatta ttcgtgccct caagattgaa ggtggatgta atgttcagct
     5221 agctcttgat cctaacagct tcaagtatta tgttatcgaa gtaaaccctc gtgtatcacg
     5281 ttcgtcagcc cttgcttcta aagcgacagg atatccgatt gctaagttgg ctgccaagat
     5341 tgcagtaggt ttgaccttgg atgaggttat aaacccagtt acaggttcaa cctatgccat
     5401 gtttgaacca gcccttgact acgtagttgc taagattcca cgtttcccat ttgacaagtt
     5461 tgaaaagggt gaacgccgtc tcggtacaca aatgaaggcg actggagaag tcatggcaat
     5521 cggtcgtaac atcgaggaat ctcttctcaa ggcctgccgc tcccttgaaa ttggggtgca
     5581 ccacaatgaa atacctgaac ttgcagcagt ttctgatgat gccttgattg aaaaggttgt
     5641 caaagcccaa gatgaccgtc tcttctacgt atcagaagcc attcgtcgtg gctacacacc
     5701 tgaagaaatt gcagagctta caaaaattga tatcttctat cttgataaac tcttgcatat
     5761 ctttgaaatt gagcaagaat tgggtgctca tccacaagat ttagaagttt tgaaaacagc
     5821 caaacttaat ggtttttcag accgtaagat tgctgaactc tggggaacga ctgatgataa
     5881 agttcgccaa cttcgcttgg aaaacaagat tgttccagtt tataagatgg tagacacttg
     5941 tgcggcagag ttcgactccg aaacgccata tttctattca acctatggtt gggaaaatga
     6001 atctatcaga tctgataaag aatctgtact tgtcctaggt tcaggtccaa tccgtatcgg
     6061 tcaaggggtt gagtttgact acgcaaccgt tcactctgtt aaggctatcc aggcagctgg
     6121 ttatgaagct atcatcatga actcaaaccc agagaccgtt tcaacagact tctctgtatc
     6181 agataagctt tactttgagc cattgacatt cgaagatgtt atgaacgtca ttgacttgga
     6241 acaaccaaag ggagttatcg ttcagttcgg tggtcaaaca gccatcaacc ttgcggagcc
     6301 attggcaaaa gcaggtgtga ccatccttgg tacacaggtt gctgacctag atcgtgccga
     6361 agaccgtgac ctcttcgagc aagctctcaa agaattggat attccacagc caccaggaca
     6421 aacggctacc aatgaagaag aagcagctct tgcagctcgc aagattggct tcccagttct
     6481 tgtccgccca tcttatgtac ttggtggacg tgccatggaa atcgttgaaa acgaagaaga
     6541 ccttcgttct tacatgcgaa ctgctgttaa ggctagtcca gaccacccag ttcttgtcga
     6601 ctcttatatc gttgggcaag agtgcgaagt tgatgccatt tcagatggta aaaatgtact
     6661 cattcctggt atcatggagc atatcgaacg tgctggtgtc cactcaggtg actcaatggc
     6721 tgtttaccca ccacaaacct tgtcgcaaaa ggttcaggag accatcgcag actacaccaa
     6781 acgcttagca atcggtcttc actgccttgg aatgatgaac atccagtttg ttatcaagga
     6841 tgaaaaagtc tacgttattg aggtcaatcc acgtgccagc cgtactgttc cattcctatc
     6901 taaggtaacc aatattccta tggctcaagt agcgaccaaa ctcattcttg gtcaaagttt
     6961 atcagaactt ggctaccaaa atggacttta ccctgaaagc acccgtgttc atatcaaggc
     7021 acctgtcttc tcctttacca aactagctaa ggtagacagc ttgctcggtc ctgaaatgaa
     7081 gtcaacaggt gaagttatgg gttctgatgc tactttggaa aaagctctct ataaagcctt
     7141 tgaagcttct tatctacact tgccaacttt tggtaatgtc gtctttacca tcgctgatga
     7201 tgccaaagaa gaagccttga acttggctcg tcgtttccaa aacattggtt atggaatcct
     7261 tgcgacagaa gggacagcag ccttctttgc tagtcatggc ttgcaagccc aacctgttgg
     7321 taagattggt gatgacgata aggatatccc aagttttgta cgcaaaggaa gaattcaagc
     7381 tatcattaac acagtaggaa caaaacgaac tgctgacgaa gatggtgagc aaattcgtcg
     7441 ttcagccatt gaacacgggg tgcccctctt cacagcgcta gatacagcta atgccatgct
     7501 taaggtactt gaaagtcgta gctttgttac agaagcgatt taaatagaaa aataagatat
     7561 agcagactga atttagaaca gtacacagg
//
LOCUS       DAVLYV010000088         7099 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLYV010000088 DAVLYV010000000
VERSION     DAVLYV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7099
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.088"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            40..909
                     /gene="rfbA"
                     /locus_tag="VYO70_001813"
     CDS             40..909
                     /gene="rfbA"
                     /locus_tag="VYO70_001813"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEV1713999.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLPRFKELLQDGSEFGIKLSYAEQPSPDGLAQAFIIGEEFIGDDSV
                     ALILGDNIYHGPGLSKMLQKAAKKEKGATVFGYHVKDPERFGVVEFDENMNAISIEEK
                     PEYPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLTLAQPLKKN
                     EYGQYLLRLIGEA"
     gene            910..1506
                     /locus_tag="VYO70_001814"
     CDS             910..1506
                     /locus_tag="VYO70_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HEV1714000.1"
                     /translation="MTDNFFGKMLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPASFFAAGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            1516..2565
                     /gene="rfbB"
                     /locus_tag="VYO70_001815"
     CDS             1516..2565
                     /gene="rfbB"
                     /locus_tag="VYO70_001815"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEV1714001.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQAAD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKTEKE
                     AVEANYAKTQEIITV"
     gene            2631..3482
                     /gene="rfbD"
                     /locus_tag="VYO70_001816"
     CDS             2631..3482
                     /gene="rfbD"
                     /locus_tag="VYO70_001816"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEV1714002.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAKMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNIAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENCKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            3545..4642
                     /gene="glf"
                     /locus_tag="VYO70_001817"
                     /pseudo
     CDS             3545..4642
                     /gene="glf"
                     /locus_tag="VYO70_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476416.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4963..6945
                     /locus_tag="VYO70_001818"
     CDS             4963..6945
                     /locus_tag="VYO70_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1714003.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMQEKEESNKKAQEDLAKHVK"
BASE COUNT         2312 a         1220 c         1557 g         2010 t
ORIGIN      
        1 taacttatga aaacaaattt aaaaaggagg gactatagta tgaaaggtat tattcttgca
       61 ggtggttcgg ggacacgatt atatcctttg actcgggctg catcaaaaca acttatgccg
      121 gtttatgata aacccatgat ttactatcca ctttcaacat tgatgttggc tgggattagg
      181 gatattttga taatctcaac tcctcaagat ttgcctcgtt ttaaagagct tcttcaagac
      241 ggatctgagt ttggtattaa actttcttat gcagagcaac caagtccaga tggtttggca
      301 caagccttta tcattgggga agagtttatt ggtgatgata gtgttgcttt gatcttgggg
      361 gacaatatct atcacggtcc tggtttgagc aaaatgcttc aaaaagcagc caagaaagag
      421 aaaggtgcga ctgtctttgg ctaccatgtc aaggatccag aacgctttgg tgttgtagaa
      481 tttgatgaaa atatgaatgc tatctccatc gaagaaaagc cagaataccc tcgttcaaac
      541 tatgcagtga caggactcta tttctatgat aatgatgttg tcgaaattgc caagagtatc
      601 aaaccaagtc ctcgcggaga attagaaatc acagatgtca acaaggctta cttggatcgt
      661 ggagacttat ccgttgagct tatgggacgt ggttttgctt ggttggatac tggcactcat
      721 gaaagtttac tagaggcttc gcagtacatc gaaacagtcc aacggatgca aaatgttcag
      781 gtagcaaact tggaagaaat tgcctatcgc atgggttata tcagtcgtga agatgtattg
      841 accttagccc aaccacttaa gaaaaatgaa tacggacagt atctgctccg tttgattgga
      901 gaagcataga tgacagataa ttttttcggt aagatgcttg cggcacgcaa ggttgaagct
      961 attccaggca tgttggagtt tgatatcccc gttcatggag ataatcgtgg ctggtttaaa
     1021 gaaaatttcc aaaaggaaaa aatgcttcca cttggatttc cagcgtcttt ctttgcagca
     1081 ggaaaattgc aaaacaatgt atccttctca cgtaaaaatg tccttcgagg cctccacgca
     1141 gagccttggg ataagtacat ctctgtagca gatggaggga aagttctggg ttcttgggtt
     1201 gatctacgcg agggtgaaac ctttgggaat acctatcaga cagtgattga tgcgagtaag
     1261 ggaatctttg ttcctcgagg cgtagctaat ggcttccaag ttttatcaga tacagtgtca
     1321 tatagctatc tggtcaatga ttactgggct cttgaactca aacccaagta tgcctttgtg
     1381 aactacgctg atccaagcct tggtattgaa tgggaaaata ttgcagaagc agaggtttca
     1441 gaagcagata aacatcatcc cctacttaag gacgtgaagc ctttgaaaaa agaagatttg
     1501 gaataaggaa agaatatgac tgaatacaaa aatattatcg tgacaggtgg agctggcttt
     1561 atcggttcta actttgtcca ttatgtttac gagaactttc cagatgttca cgtgacagtc
     1621 ctagataagt tgacttatgc tggaaaccgc gcgaatattg aggaaatttt aggtaatcgt
     1681 gttgagttag ttgttggtga cattgctgat gcggagttgg tagacaagtt ggctgctcaa
     1741 gcagatgcta tcgttcatta tgcagcggaa agccacaatg ataattcgct caatgatcca
     1801 tcgccattta ttcatactaa cttcatcgga acctatactc ttttagaagc tgctcgtaag
     1861 tatgatattc gcttccacca tgtatcgaca gatgaagttt atggagatct ccctttacgc
     1921 gaagatttgc caggtcatgg agaagggccg ggtgagaaat ttacggctga aaccaagtac
     1981 aatccaagct cgccttactc atcaaccaag gcagcctcag atttgattgt caaagcctgg
     2041 gtgcgttctt ttggagtcaa ggcaacgatt tccaactgtt caaataacta cggtccttat
     2101 caacatatcg aaaaattcat cccacgtcag attactaaca tcctaagtgg tatcaagcca
     2161 aaactttacg gtgaaggtaa aaacgttcgt gactggattc ataccaatga ccattcttca
     2221 ggagtttgga caatcttgac aaaagggcaa atcggtgaaa cctacttgat tggggctgat
     2281 ggtgagaaga acaataagga agttttggaa cttatcctta aggaaatggg acaagctgcg
     2341 gatgcctatg atcatgtgac tgaccgtgca ggacatgacc ttcgctatgc gattgatgct
     2401 agcaagctcc gtgatgagtt ggggtggaaa cctgaattta ccaactttga agctgggctc
     2461 aaggcaacaa tcaagtggta tacagataac caagaatggt ggaaaacaga aaaagaagct
     2521 gttgaagcca attatgctaa gactcaggag attattacag tataaaaagc aggaaatagc
     2581 tgctttttat tgctatattg ggaagagtta catattagaa aggtctagag atgattttaa
     2641 ttacaggggc aaatggccaa ttaggaacgg aacttcgcta tttattggat gaacgtaatg
     2701 aagaatacgt ggcagtagat gtggctaaga tggacattac caatgaagaa atggttgaga
     2761 aagtttttga agaggtgaaa ccgactttag tctaccattg tgcagcctac accgctgttg
     2821 atgcagcaga ggatgaagga aaagagttgg acttcgccat caatgtgacg gggacaaaaa
     2881 atatcgcaaa agcatctgaa aagcatggtg caactctagt ttatatttct acggactatg
     2941 tctttgacgg taagaaacca gttggacaag agtgggaagt tgatgaccga ccagatccac
     3001 agacagaata tggacgcact aagcgtatgg gggaagagtt agttgagaag catgtgtcta
     3061 atttctatat tatccgtact gcctgggtat ttggaaatta tggcaaaaac ttcgttttta
     3121 ccatgcaaaa tcttgcgaaa actcataaga ctttaacagt tgtaaatgac cagtacggtc
     3181 gtccgacttg gactcgtacc ttggctgagt tcatgaccta cctagctgaa aattgtaagg
     3241 aatttggtta ttatcatttg tcaaatgatg cgacagaaga tacaacatgg tatgattttg
     3301 cagttgaaat tttgaaagat acagatgtcg aagtcaagcc agtagattcc agtcaatttc
     3361 cagccaaagc taaacgtccg ctaaactcaa cgatgagcct ggccaaagcc aaagctactg
     3421 gatttgttat tccaacttgg caagatgcat tgcaagaatt ttacaaacaa gaagtgagat
     3481 aagtagtaga atgattttct agtctaataa aagaggcaga taatgaactc caaaggagct
     3541 taagatatac aattatcttg ttgttggtgc tggtctcttt ggtgcagtct ttgcccatga
     3601 agcagcctta aaaggaaaaa agtaaaagtt attgaaaaac gaaatcatat cgcgggtaat
     3661 atctatactc gtgaagagga aggaattcaa gttcatcaat atggtgctca tatcttccat
     3721 acttctgata aggagatctg ggagtatgta aatcagtttg cagagtttaa ccgttatacc
     3781 aattctcctg ttgcaaacta taagggtgag atttataacc ttccttttaa tatgaatact
     3841 ttcaataaac tctggggagt tgtaacgcca gcagaagcac aagctaagat tgaggaacaa
     3901 cgtgctattt taaatggtaa aactcctgaa aatttggaag aacaggcgat ttctcttgta
     3961 ggtacagaca tctacgaaaa attaatcaaa gactatacag agaaacagtg gggcaaatca
     4021 actactgaac ttccatcctt tattattgtt tgccagtacg cctgacctat gataacaact
     4081 attttaacga tacctatcaa gggattccaa ttggtggata cactcaaata gttgaaaaat
     4141 gttggatcat gaaaatattg atgtagaaac aaacgttgat ttctttgtga ataaagagca
     4201 atatctgaaa gattttccta agattgtctt tactggtatg attgatgaat tctttgacta
     4261 taagttgggc gaactagagt actgtagtct tcgttttgaa aatgagacct tggatatgga
     4321 aaattaccaa ggaaatgcag ttgtgaacta tacggatgca gaaaccccat atactcgcat
     4381 tattgaacac aaacattttg agtttgggag tcaagcaaag actatcatta ctaaagaaca
     4441 ttctaaaaca tgggaaaaag gtgatgagcc ttattatcca gttaataatg atcgtaataa
     4501 tcatttgtat aaatcgtata aaaaacttgc tgatgagcaa gggaatgtta tctttggtgg
     4561 tcgcttagga cactatcgtt attacgatat gcaccaagta attggagcag ctttgcagtg
     4621 cgtgagaaat gagttagatt aatactcaat gaaaatcaaa gagcaaacta ggaagctagc
     4681 cacaggttgc tcaaaatact gttttgaggt tgcagatgga agctgacgcg gtttgaagag
     4741 attttcgaag agtataaaca agtaaaactg actaccagtt attatttaga aatagtatta
     4801 aaaattcctt gactatgtga tatagttgag ggatttttaa atgatattca tattttttgc
     4861 aaagatgttg tttgaaaaat aattttcaaa aattctgaaa attctgttga caactttctg
     4921 aaaagagtct ataatggaga gaaagtttta aaggagaaaa tgatgaaaag ttcaaaacta
     4981 tttgcccttg cgggcgtgac attattggcg gcgactactt tagctgcatg ctctggatca
     5041 ggttcaagca ctaaaggtga gaagacattc tcatacattt atgagacaga ccctgataac
     5101 ctcaactatt tgacaactgc taaggctgcg acagcaaata ttaccagtaa cgtggttgat
     5161 ggtttgctag aaaatgatcg ctacgggaac tttgtgccgt ctatggctga ggattggtct
     5221 gtatccaagg atggattgac ttacacttat actatccgta aggatgcaaa atggtatact
     5281 tctgaaggtg aagaatacgc ggcagtcaaa gctcaagact ttgtaacagg actaaaatat
     5341 gctgctgata aaaaatcaga tgctctttac cttgttcaag aatcaatcaa agggttggat
     5401 gcctatgtaa aaggggaaat caaagatttc tcacaagtag gaattaaggc tctggatgaa
     5461 cagacagttc agtacacttt gaacaaacca gaaagcttct ggaattctaa gacaaccatg
     5521 ggtgtgcttg cgccagttaa tgaagagttt ttgaattcaa aaggagatga ttttgccaaa
     5581 gctacggatc caagtagtct cttgtataac ggtccttatt tgttgaaatc cattgtgacc
     5641 aaatcttctg ttgaatttgc gaaaaatccg aactactggg ataaggataa tgtgcatatt
     5701 gacaaagtta aattgtcatt ctgggatggt caagatacca gcaaacctgc agaaaacttt
     5761 aaagatggta gccttacagc agctcgtctc tatccaacaa gtgcaagttt tgcagagctt
     5821 gagaagagta tgaaggacaa tattgtctat actcaacaag actctattac gtatctagtt
     5881 ggtacaaata ttgaccgtca gtcctataaa tacacatcta agaccagcga cgaacaaaag
     5941 gcatcgacta aaaaggctct cttaaacaag gatttccgtc aggctattgc ctttggtttt
     6001 gatcgtacag cctatgcctc tcagttgaat ggacaaactg gagcaagcaa aatcttacgt
     6061 aatatctttg ttccaccaac atttgttcaa gcagatggta aaaactttgg cgatatggtc
     6121 aaagagaaat tggttactta tggggatgaa tggaaggatg ttaatcttgc agattctcag
     6181 gatggtcttt acaatccaga aaaagccaag gctgaatttg ctaaagctaa atcagcctta
     6241 caagcagaag gtgtgacttt cccaattcat ttggatatgc cagttgacca gacagcaact
     6301 acaaaagttc agcgcgtcca atctatgaaa caatccttgg aagcaacttt aggagctgat
     6361 aatgtcatta ttgatatcca acaactacaa aaagacgaag taaacaatat tacatatttt
     6421 gctgaaaatg ctgctggcga agactgggat ttatcagata atgtcggttg gggtccagac
     6481 tttgccgatc catcaaccta ccttgatatc atcaaaccat ctgtaggaga aagtactaaa
     6541 acatatttag ggtttgactc aggggaagat aatgtagctg ctaaaaaagt aggtctatat
     6601 gactacgaaa aattggttac tgaggctggt gatgaggcta cagatgttgc taaacgctat
     6661 gataaatacg ctgcagccca agcttggttg acagatagtg ctttgattat tccaactaca
     6721 tctcgtacag ggcgtccaat cttgtctaag atggtaccat ttacaatacc atttgcattg
     6781 tcaggaaata aaggtacaag tgaaccagtc ttgtataaat acttggaact tcaagacaag
     6841 gcagtcactg tagatgaata ccaaaaagct caggaaaaat ggatgcaaga gaaagaagag
     6901 tctaataaaa aggctcaaga agatctcgca aaacatgtga aataactgtt gcaaaatata
     6961 agaaaggatt tagtatttct cttgaatgct gaatcctttt ttacatttgt aaagaaagat
     7021 tctaaatgta ctgaccccca aaagttggag cctctttttg tcagaataga gaaaattttt
     7081 gttaatttta cttgtttcc
//
LOCUS       DAVLYV010000089         6935 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLYV010000089 DAVLYV010000000
VERSION     DAVLYV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.089"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(93..389)
                     /locus_tag="VYO70_001819"
     CDS             complement(93..389)
                     /locus_tag="VYO70_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1714004.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(379..540)
                     /locus_tag="VYO70_001820"
     CDS             complement(379..540)
                     /locus_tag="VYO70_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HEV1714005.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            610..1374
                     /locus_tag="VYO70_001821"
     CDS             610..1374
                     /locus_tag="VYO70_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1714006.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(1614..1766)
                     /locus_tag="VYO70_001822"
     CDS             complement(1614..1766)
                     /locus_tag="VYO70_001822"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714007.1"
                     /translation="MKIKASIRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NISTAR"
     gene            1984..3363
                     /locus_tag="VYO70_001823"
     CDS             1984..3363
                     /locus_tag="VYO70_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1714008.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            3377..4042
                     /locus_tag="VYO70_001824"
     CDS             3377..4042
                     /locus_tag="VYO70_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1714009.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            4177..4251
                     /locus_tag="VYO70_001825"
                     /pseudo
     CDS             4177..4251
                     /locus_tag="VYO70_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            4397..4936
                     /locus_tag="VYO70_001826"
     CDS             4397..4936
                     /locus_tag="VYO70_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1714010.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKGHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YIMMHSLIYRTDLLRASQF"
     gene            5036..5413
                     /locus_tag="VYO70_001827"
     CDS             5036..5413
                     /locus_tag="VYO70_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1714011.1"
                     /translation="MIGREDQSVNEQVMIKCIDQQLKVNRLLVDQLDLSQVSHPKMRE
                     YLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQENPKVFQAIRKTMLSRLTKH
                     SVLPDRKLSNVVYQITKSVYGFN"
     gene            5529..>6935
                     /gene="pavB"
                     /locus_tag="VYO70_001828"
     CDS             5529..>6935
                     /gene="pavB"
                     /locus_tag="VYO70_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1714012.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADG
                     LNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQENPELTDALKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVQKAVADNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALD
                     GNVAGKEKQALIDQFRANGTQTYSATVNVYGNKDGKPDLDNIVATKKVTININGLISK
                     ETVQKAVADNVKDSIDVPA"
BASE COUNT         1915 a         1342 c         1594 g         2084 t
ORIGIN      
        1 gcctgcggct aacttcctag tttgcacttt aattttcatt gagtattact tcatcttctc
       61 atgaaattga gtttttgccc agctcctctt gattattcta cttgaccggg ccaagcattc
      121 ataccacctt ctacgttggt aacggtgagg ccttgggagc tgagaaattg gcaggtagat
      181 gcagagcgga ctcctccttg acaaatgaca tagtattcat ggtcaggttt gagttctttg
      241 tagccttgct ctaaggtact taatggaaga tttttggcac ctggtgcatg tcctgcttgg
      301 aattcatgtg cttcacggac atcgataagt tctagatttt cattttggta ttttccataa
      361 aagtcagcca tgctgatact agtttccata ttctactcct tctgggttaa ccattttgta
      421 tagtgaataa gcgccgtcaa ggttttgggc ggtaaatcct gcttgcttga ggataggctc
      481 tgcgatatag ctgcgcaaac cactgtggca gctgacgatg taggcttgtt ttttgtccag
      541 ttcatcagct tcctccttga cactttgtca gtttttgata caatagtaca aaattagagg
      601 aggcaggcta tgattcagaa acatgcgatt cctattttag agtttgatga caatcctcag
      661 gcggttatca tgcccaatca cgaggggctg gacttgcagt tgccaaagaa gtgtgtttat
      721 gcatttttag gtgaggagat tgaccgctat gcaagggaag taggggcgga ttgtgtcggc
      781 gaattcgttt ctgctaccaa gacctatcca gtctatgtca tcaactacaa gggtgaggag
      841 gtctgtctgg ctcaggctcc tgttggctcc gctccagcag cccagtttat ggattggttg
      901 attggctatg gtgtggagca gattatctct actgggacct gtggtgtcct agctgatata
      961 gaggaaaatg cctttctagt ccctgttcgc gctttgcgag atgagggagc cagttaccac
     1021 tatgtggcac cttgtcgtta tatggaaatg cagccagagg ctattgctgc tattgaggaa
     1081 gttttggaag acagagggat tccttatgaa gaagtcatga cctggacgac agacggtttt
     1141 taccgagaaa cggctgaaaa ggtggcttat cgtaaggaag aaggctgtgc tgttgtggag
     1201 atggagtgtt ctgctcttgc ggcagtagct caattgcgtg gggttctctg gggtgaattg
     1261 ttgttcacag cagattctct agcggacttg gaccagtacg acagtcgtga ctggggctcg
     1321 gaagctttta ataaggcgct agaactgagt ttagcaagtg ttcaccacct ttagttgtac
     1381 tggcaaagga tttgttttat cataaaatgt ctagctcata cttttcaaaa atatgtttaa
     1441 acgaggtcac cttcctcttg tcctaggcat gttgaggttg ggaaaaatct ttaaaatcag
     1501 aaaaacgtat catatcaggt gatgaaaact ttgatactat gcgttttatg ttggtaagat
     1561 ttagagtgag atgaaatgat actcttcgaa aatcttttca aaccaggtca gcttcacctt
     1621 gccgtagata tgttactgac ttcgtcagtt ctatctgcaa cctcaaaaca gtgttttgag
     1681 ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagcaacc tgtgactagc
     1741 tttctaatcg atgccttgat tttcattgcc tataatcaaa aagagaaatt ttctcctgaa
     1801 aagcatatag agtagctggc gttaaaagct cctgtcttgc ttttttgacc tatagtcaca
     1861 tctatcaagt attgttcttg cctaagctat caataaaaag gtggcatttt ttaggcttgg
     1921 tgttagtgaa ttttgcctta tcctatctaa gtcatttcga gctttttatg gtacaatgga
     1981 aacatgttat tcaaattatc taaggaaaaa atagagctag gcttatctcg tttatcgcca
     2041 gcccgtcgta tttttttgag ttttgccttg gtcattttac taggctctct tcttttgagc
     2101 ttgccctttg tccaagttga aagctcacga gcgacttatt ttgatcatct tttcactgct
     2161 gtctctgcag tctgtgtgac aggtctctca acccttccag tagctcacac ctataatatc
     2221 tggggccaaa taatctgttt gctcttgatt cagatcggtg gtctagggct catgactttt
     2281 attggggttt tctatatcca gagcaagcaa aagcttagtc ttcgtagccg tgcaactatt
     2341 caggatagtt ttagttatgg agaaactcga tctttgagaa agtttgtcta ttctattttt
     2401 ctcacgacct ttttggttga gagcttggga gctattttgc ttagttttcg ccttattcct
     2461 caacttggct ggggacgtgg tctttttagt tccatttttc tagcgatctc agccttctgt
     2521 aatgccggtt ttgataattt agggagcacc agtttatttg cttttcagac cgatttactg
     2581 gtcaatctgg tgattgcagg cttgattatt acaggcggcc ttggttttat ggtctggttt
     2641 gatttggctg gtcatgtagg aagaaagaaa aaaggacgtc tgcactttca tacgaagctt
     2701 gtactattat tgactatagg tttgttgtta tttggaacag caactactct ctttcttgag
     2761 tggaacaatg ctggaacgat tggcaatctc cctgttgccg ataaggtttt agttagcttt
     2821 tttcaaacag tgacgatgcg aacagctggc ttttctacga tagattatac tcaggctcat
     2881 cctgtgactc ttttgattta tatcttacag atgtttctag gtggggcacc tggaggaaca
     2941 gctgggggac tcaagattac gacatttttt gtcctcttgg tctttgcacg aagtgagctt
     3001 ctaggcttgc ctcatgccaa tgttgcgaga cgaacgatcg cgccgcgaac ggttcaaaaa
     3061 tcctttagtg tctttattat ctttttgatg agcttcttga taggattgat tctgctaggg
     3121 ataacagcca aaggcaatcc tccctttatc cacctcgtat ttgaaaccat ttcagctctt
     3181 agtacagttg gtgtaacggc aaatctgact cctgaccttg ggaaattggc tctcagtgtt
     3241 atcatgccac ttatgtttat gggacgaatt ggtcccttga ccttgtttgt tagcttggca
     3301 gattaccatc cagaaaagaa agatatgatt cactatatga aagcagatat tagtattggt
     3361 taagaaagga aagagcatgt cagatcgtac gattggaatt ttgggcttgg gaatttttgg
     3421 gagcagtgtc ctagctgccc tagccaagca ggatatgaat attatcgcta ttgatgacca
     3481 cgcagagcgc atcaatcagt ttgagccagt tttggcgcgt ggagtgattg gtgacatcac
     3541 agatgaagaa ttattgagat cagcagggat tgatacctgc gataccgttg tagtcgcgac
     3601 aggtgaaaat ctggagtcga gtgtgcttgc ggttatgcac tgtaagagtt tgggggtacc
     3661 gactgttatt gctaaggtca aaagtcagac cgctaagaaa gtgctagaaa agattggagc
     3721 tgactcggtt atctcgccag agtatgaaat ggggcagtct ctagcacaga ccattctttt
     3781 ccataatagt gttgatgtct ttcagttgga taaaaatgtg tctatcgtgg agatgaaaat
     3841 tcctcagtct tgggcaggtc aaagtctgag taaattagac ctccgtggca aatacaatct
     3901 gaatattttg ggtttccgag agcaggaaaa ttccccattg gatgttgaat ttggaccaga
     3961 tgacctcttg aaagcagata cctatatttt ggcagtcatc aacaaccagt atttggatac
     4021 cctagtagca ttgaattcgt aaagagggat gacccctctt ttttgatgcc caagatggca
     4081 aatagagaca gaagcccctt gtcttctagt aaaagttctt caaaggctgg actttatggt
     4141 aaaatagaaa gaagtgacaa gagagagtaa tactcaatga aaatcaaaga tcaaactagg
     4201 aaactagcta cgggctgctc aaaacagtgt tttgaggttg tggatagaac tgacgaagtc
     4261 agctcaaaac actgttttga ggttgcagat agaactgacg aagtcagtaa catctatacg
     4321 gcaaggcgac gttgacgcgg tttgaagaga ttttcgaaga gtataagaaa aaatcagtcc
     4381 cctaaaggag cagattatga agttattgtc tatcgccatt tctagctata atgcagcagc
     4441 ctatcttcat tactgtgtgg agtcgctagt gattggtggt gagcaagttg ggattttgat
     4501 tatcaatgac gggtctcagg atcagactca ggaaatcgct gagtgtttag ctagcaagta
     4561 tcctaatatc gttagagcca tctatcagga aaataaaggc catggcggtg cggtcaatcg
     4621 tggcttggca gaggcttctg ggcgctattt taaagtagtt gacagtgatg actgggtgga
     4681 tcctcgtgcc tacttgaaaa ttcttgaaac cttgcaggaa cttgagagca aaggtcaaga
     4741 ggtggatgtc tttgtgacca attttgtcta tgaaaaggaa gggcagtctc gtaagaagag
     4801 tatgagttac gattcagtct tgcctgttcg gcagattttt ggctgggacc aggtcggaaa
     4861 tttctccaaa ggccagtata tcatgatgca ctcgctgatt tatcggacag atttgttgcg
     4921 tgctagccag ttctaactgc ctgaacatac tttttatgtc gataatctct ttgtctttac
     4981 gccccttcag caggtcaaga ccaggtacta tctgcctgtc gatttctatc gttatttgat
     5041 tgggcgtgag gaccagtctg tcaatgagca agtgatgatt aagtgcattg accagcaact
     5101 caaggtcaat cgactcttgg tagaccagct tgatttgtcc caagtgagtc atcctaaaat
     5161 gcgagaatat ctgctgaatc atattgaaat cacgacggtg atttccagta ccctgctcaa
     5221 ccgatctgaa acagcggagc atctggcaaa aaaacgccaa ttgtggacct atattcagca
     5281 ggaaaatcca aaagtctttc aggctattcg taagaccatg ttgagccgtt tgaccaaaca
     5341 ttctgtcttg ccagatcgca aactgtccaa tgtcgtctat caaatcacca aatctgttta
     5401 tggatttaat taatataagt gttttataag agggatttaa gaaaaatttt aactttttct
     5461 tagtcctttt taatttcagg agattatact agagtcatca aataaagaaa gactctaagg
     5521 agaatcctat gaaattcaat ccaaatcaaa gatatactcg ttggtctatt cgccgtctca
     5581 gtgtcggtgt tgcctcagtt gttgtggcta gtggcttctt tgtcctagtt ggtcagccaa
     5641 gttctgtacg tgccgatggg ctcaatccga ccccaggtca agtcttacct gaagagacat
     5701 cgggaacgaa agagggtgac ttatcagaaa aaccaggaga caccgttctc actcaagcga
     5761 aacctgaggg cgttactgga aatacgaatt cacttccgac acctacagaa agaactgaag
     5821 tgagcgagga aacaaactct tctagtctgg atacactttt tgaaaaagat gaagaagctc
     5881 aagaaaatcc agagctaaca gatgccttaa aagaaactgt agatacagct gatgtggatg
     5941 ggacacaagc aagtccagca gaaactactc ctgaacaagt aaaaggtgga gtgaaagaaa
     6001 atacaaaaga cagcatcgat gttcctgctg cttatcttga aaaagctgaa gggaaaggtc
     6061 ctttcactgc cggtgtaaac caagtaattc cttatgaact attcgctggt gatggtatgt
     6121 taactcgtct attactaaaa gcttcggata atgctccttg gtctgacaat ggtactgcta
     6181 aaaatcctgc tttacctcct cttgaaggat taacaaaagg gaaatacttc tatgaagtag
     6241 acttaaatgg caatactgtt ggtaaacaag gtcaagcttt aattgatcaa cttcgcgcta
     6301 atggtactca aacttataaa gctactgtta aagtttacgg aaataaagac ggtaaagctg
     6361 acttgactaa tctagttgct actaaaaatg tagacatcaa catcaatgga ttagttgcta
     6421 aagaaacagt tcaaaaagcc gttgcagaca acgttaagga cagtatcgat gttccagcag
     6481 cctacttaga aaaagccaag ggtgaaggtc cattcacagc aggtgtcaac catgtgattc
     6541 catacgaact cttcgcaggt gatggcatgt tgactcgtct cttgctcaag gcatctgaca
     6601 aggcaccatg gtcagataac ggtgacgcta aaaacccagc cctatctcca ctaggtgaaa
     6661 acgtgaagac caaaggtcaa tacttctatc aagtagcctt ggacggaaat gtagctggca
     6721 aagaaaaaca agcgctcatt gaccagttcc gagcaaacgg tactcaaact tacagcgcta
     6781 cagtcaatgt ctatggtaac aaagacggta aaccagactt ggacaacatc gtagcaacta
     6841 aaaaagtcac tattaacata aacggtttaa tttctaaaga aacagttcaa aaagccgttg
     6901 cagacaacgt taaagacagt atcgatgttc cagca
//
LOCUS       DAVLYV010000090         6739 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLYV010000090 DAVLYV010000000
VERSION     DAVLYV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.090"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(34..810)
                     /locus_tag="VYO70_001829"
     CDS             complement(34..810)
                     /locus_tag="VYO70_001829"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1714013.1"
                     /translation="MPKTLTEKLNAIKVTGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(803..2026)
                     /gene="trpB"
                     /locus_tag="VYO70_001830"
     CDS             complement(803..2026)
                     /gene="trpB"
                     /locus_tag="VYO70_001830"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1714014.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGSPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(2004..2603)
                     /locus_tag="VYO70_001831"
     CDS             complement(2004..2603)
                     /locus_tag="VYO70_001831"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1714015.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEVAE
                     LAKLIPADVKKVGVFVSPSRAELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            complement(2590..3357)
                     /gene="trpC"
                     /locus_tag="VYO70_001832"
     CDS             complement(2590..3357)
                     /gene="trpC"
                     /locus_tag="VYO70_001832"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1714016.1"
                     /translation="MSQEFLARILEQKVREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(3354..4358)
                     /gene="trpD"
                     /locus_tag="VYO70_001833"
     CDS             complement(3354..4358)
                     /gene="trpD"
                     /locus_tag="VYO70_001833"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1714017.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(4369..4935)
                     /locus_tag="VYO70_001834"
     CDS             complement(4369..4935)
                     /locus_tag="VYO70_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1714018.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(4932..6293)
                     /gene="trpE"
                     /locus_tag="VYO70_001835"
     CDS             complement(4932..6293)
                     /gene="trpE"
                     /locus_tag="VYO70_001835"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1714019.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFYILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
BASE COUNT         1760 a         1696 c         1354 g         1929 t
ORIGIN      
        1 tcgtgccttt cctcttactt gctacttgtt tgattatttt tggtaagcta ctgcttgtct
       61 gataaaatcc tgaatcggct ctccttggtg gagagctttt actatttttg aaccgacgat
      121 aacaccatct gacaccgcat tgaagcgttc cagatcggct tgactagata caccaaaacc
      181 tgtcaagact gggatgtcgg ccacttgatg aagttgcgcc aagtgcttgt ccaaatctgc
      241 acggtaattg cctgatttcc ctgtcacccc attgatggca acggcataga cgaatccctc
      301 cgccccttca atcaactctt tctggcgctc aattcctgtg gtcaagctta ctaaaggaat
      361 caaggcgata tctgtatctg ccaaaaatgg ttctacaaag ttggcatgtt catgaggcag
      421 gtctgggata atcaagccct tcacagctgt atcagccaga tctttgacaa agttctccac
      481 accgtactga aagagggggt tgaagtaggt catgatgacc agtggaatct ctgtttcaat
      541 ggttttcaag gtttcaacta aagcctgggt agaggtcccg tgggctaaac tgcgcaagcc
      601 agcttcttcg ataacaggtc catctgcaac agggtctgaa aagggaatcc ccacttcaat
      661 agcagagaca cccaaatctt ctaaaaagtg aattgtttca gcaagaccgt ccaaaccttt
      721 ttcgtggtca ccagccatga tataaggaac gaaaattcct tttccagtta cttttatagc
      781 attcaatttt tctgttagtg tcttaggcat gagcttctcc cttctttgct gcatctgctt
      841 ccaagcggtc tttgacttga accacatcct tgtccccacg acctgatagg cagacaatca
      901 tagacttttc tggtccaagt tctttggcca atttcaccgc aaaagcgata gcatggctag
      961 attccaaggc tgggataatc ccttccacac gagacaagag ttgaaatcct tccaaagctt
     1021 cttcgtctgt cacagggaca tagctggcac gtttgatatc gtggtagtga gaatgctctg
     1081 gaccgatacc aggatagtcc aaacctgctg agatagagaa ggcttcaaga atttgaccat
     1141 gggcatcttg gagcacatcc atgagagaac cgtgaagaac acccggacta cccttggtca
     1201 aggtagcagc gtggtgctct gtatccacac caagccctgc tgcttcagtt ccatacatag
     1261 ctactgactc atcttctaca aagggatgga agagcccgat agcattcgac ccaccaccaa
     1321 cacaggctac tagggcatct ggcagatctc gacctgtcaa gtcacggtac tgttgtttag
     1381 cctctcgacc gatgacactt tggaagtcac gaacgatttc tggaaatgga tgaggcccca
     1441 aggcagaacc aaggatatag tgggtatcgt cgatattagc cacccatgaa cgaagggctg
     1501 cattgaccgc atccttgagc acgcgcgaac catctgtcac ggcctcgacc ttagctccca
     1561 aaagctccat acggaagaca ttgagggctt ggcgtttgac atcttcctca cccatgtaga
     1621 tggtacattc catgttaaag agggccgcag cagttgcagt tgccacaccg tgctgaccag
     1681 cacccgtttc tgcgataatt ttctttttac ccatgcgttt ggcaagccaa acttgtccta
     1741 aggcattgtt aatcttgtgg gctcctgtat ggttaaggtc ttcccgtttg agataaatct
     1801 tggctccgcc gatatgctgg gtcaagtttt ttgcgtagta aaggggagtt tcacgtccca
     1861 catactggcg caaaagctgg tttaattcct cttggaaact tgggtctgcc tgactttcac
     1921 ggtaggcctt ctccaactcc aaaactgctg tcatcaatgt ttctgggaca aaacgtccgc
     1981 caaattttcc gtaaaatcca tctttatttg gttcttgata tgccattctt taccctctct
     2041 ataaatcttc taatcttttc atgatctttt tgtccatctg tctccactcc gcttgataca
     2101 tctactgcat agggagtaaa gtgttgaatt gcttttacta cattatcttc attaaggcca
     2161 cctgcgataa agaagggctg tgctagtcca gtcgtatcca gttgacccca atcaaagggc
     2221 tggccactcc cagccacagg ggcatcaaag agtagatagt ctgcctgaga attgggcaca
     2281 tgaccctctc catctacctg cacagcctga atgctagcac aaggcaaatt ctcaaataaa
     2341 tcatctgcca cctgaccgtg aacttgaacc aagtccaagc caactttgtc aatcgcttcc
     2401 agcagttctg cccgacttgg tgaaacaaat acaccaacct ttttgacatc tgcaggaata
     2461 agctttgcca actcagctac ttcttctaaa gttacctgtc ttttactagg tgcaaagaca
     2521 aaaccgatat agtcggctcc tgctgaaacg gctgtttcca ccgcttcttt ggtcgatagt
     2581 ccacaaattt taacctttgt caatctgcaa ctccttgatt ctctgggcca cattttctgc
     2641 ctgcataaga gctgtcccta ccaaaattcc gttaaagtat ggggctagtc gttccgcatc
     2701 ctgccctgtg aaaatggcag attcagaaat gtaatagcga ccttcctcaa agtaaggggc
     2761 taaatctaca ctggtctgca agtcgacctc aaaggtagtc aagttgcggt tgttgacccc
     2821 gataatctca gcaccaagtc tgtgggctac ctctagttca gctagattgt gagtctccac
     2881 taagacttcc agaccaagct ctgtcgcgta gtcatacagt tccttgaggc gttcttcgga
     2941 caaggctgcc acaatgagca agataactgt cgcacctgca ttgcgagcgc ggatgatttg
     3001 cttttcatcg atgataaagt ctttgttgag cgtcggaatc tctacctgac tggaaatttc
     3061 ccgtagataa tccaaatgcc ctttaaagaa aacctcatct gtcaacaccg aaatcatcac
     3121 tgctccgttt tcttcataag tctgggcctg ttgcacaata tccacatcga gattgatatc
     3181 tcccaaacta gggctagctt tcttgacctc agcgattacc tgcaagcggt cctgatgatt
     3241 cttcaaaaac tctgccaagc gataggtctg gcgcagaggc tggatttgct ccagcttcat
     3301 ctgctccacc tcacgcacct tctgctctaa gattcgtgct aaaaattcct gactcatttt
     3361 tggtactcct gtaacagtct gagtttttca agggccttgc ctctagcaat cacttgacgg
     3421 gccaaggcaa ctccttcctt gatgctatca atcttaccat tagcatagaa accaagacca
     3481 gcattcaaga ctgtcgtttc caagaatgga cttgcttcgt ttttcagaac gctaagcaaa
     3541 atttctgcat tttcctgagc attcccacca cgaatatctt ccatagcata gccttccatt
     3601 cccaaatcct ctggagtaaa gcttgacaag ctgatttcgc cattttcaag aagtgcaatc
     3661 ttggttgttc cgttcaagcc agcttcatcc aacccttctg gtccagcaac cacgatggca
     3721 cgtttgcgac ccatattttt caaaacctga gctgtacttt ctaggagttc tggacgacta
     3781 attccaagaa gctgtgtttc taaagccatt ggatgaatca gtggaccagt caagttcata
     3841 atcgttggaa ttcccaattc caaacgagct ggcatgatgt atttcatagc tgggtgcata
     3901 tttttagcga agagaaagac gattccagtt ttatcaaaga ccttacctag ttcagctggt
     3961 ttgaggtcaa gattgattcc caaggcttcg aggacatctg cggaaccaga tttagaagaa
     4021 atcgagcggt taccatgctt ggccatgtga ataccgccac cagccaagac aaaggctgca
     4081 gttgtggaaa tattaaaact gaaagacttg tccccacctg taccacagtt gtccatggca
     4141 tcatgaatct cagttggaat atgctgggca tgtcctctca tgacttgggc aatggctgtg
     4201 cgttcttcag gtgtttcccc cttcatctta agagctaaga ggagagaagc aatctgcgct
     4261 tcagttacac gcccagttac gatacgctca atgacatccg tcatttccac acctgataaa
     4321 ttttcaaatt ttgctagttt ttcaataatc tctttcatcc tagtttcctc actttacaac
     4381 cttctcgata aaattccgaa tagaagacaa gccgtctggc gttccaatgc tctctggatg
     4441 gtactggaag ccataaatcg gtaggttttt atgttgaatc cccatgatgg cttggtcatc
     4501 agtcgaacga gctgtcactt caaagtcttc tggcatttcc tcaatcaaaa tactgtgata
     4561 acgcatgacc gcacgtcctt cctcaatacc ttgatacaaa acagatggcg cttcaaagtt
     4621 gatattgctc tgtttcccat gcatgacttt tggagccaaa cctagcttac caccaaagac
     4681 ttctgcaatg gcttggtggc ccaaacaaat cccaagaatc ggcttcttgc ctgcaaaatc
     4741 acgaatcatg tcttccatct ttccagcatc aactggccaa ccaggaccag gagaaaaaac
     4801 cagaccatct gctttttcag cttcttcata cagcttggaa tcatcatttc tcagaacctg
     4861 aacttctgca aaattcccaa tgtattgggc caagttatag gtaaaagaat catagttgtc
     4921 aatcaataaa atcatggtct tagttctcca attctagtca tagattttgc tttgttaatg
     4981 gtttcttggt attcgttttg ggcgatagag tcgtagacaa tccctgcccc agcctgcaca
     5041 taggctcttt gatttttgag aatcatggtt cggatggcaa tggccaaatc catatcaccc
     5101 gtcgcagaca agtagccgat tgctcctgca tatacgcccc gtttttccgt ttccagttca
     5161 tagatgcgtc tcatcgcccg aatctttggt gctccagaaa cggttccagc aggaagtgta
     5221 gctttcaagg catccatggc cgtgagttct ggaagcaaac gccccttgac cacgctggtc
     5281 aaatgcatga cgtagcggaa gagctccacc ttcatatact tagtaacttg gacactggcc
     5341 gtttcagaga tgcggccaat atcgttacgc cccaagtcta ccaacattcg atgttctgct
     5401 gtttccttct catcagagag gaggtcagtc gccaaggtct tgtcttcttc atccgtagcc
     5461 cctcttggtc gcgtccctgc aatcggattg gttgtcacga tgccattttt gacagaaact
     5521 aaactttctg gactagctcc gatgatttga taatccccaa aatcatagaa ataaaggtaa
     5581 ttagaaggat tagtcacgcg gagatttctg tagaagtcaa atggatttcc agtaacttct
     5641 gctgaaaaac gctggctgag tacacattgg aacatatctc cgttacgaat caagtcacga
     5701 gctgtttcta ccattccctc aaacttatgt ggagcgatat gcggtttgaa gtctaacgga
     5761 gatagatcca aatcttcaaa ttcatttgga gcaggaatgc gtaattcctc aagcacttgg
     5821 ttcaaggatt tttccaaggc ctcttgactg cgctcactat aaagtgcatc ctctatgaca
     5881 tgtatcttct ccttcttgtg gtcaaagacc atatagctct catagacaaa gaaatgcatg
     5941 tctggcgtcc caattgtatc ctcagggatt tgaccaattt cttcataaag cgaaatcata
     6001 tcgtaaccca caaaaccaat ggctccacca ccaaaaggta gctctgagtg gtgctgactc
     6061 ttatgaatca cttcataaag gaaatccaag ggatcccgat caatcacttg accattttga
     6121 tagagaaccc cattttcaaa cttaatctca aaaactggat tataggctag gatataaaaa
     6181 cgagctgttt ccttgtctct cggaatactc tctaaaataa ccttatgttg cccctttaag
     6241 cgcatataag ccaagattgg tgataagaca tctccatgaa tgattcgttc cattgtaatt
     6301 tccctttcag ttctacttct agtccgtggt gactgtatga aaaatcccca cgcaaaataa
     6361 cttgcgtgag gacgaaattc gcggtgccac ctcaattata ggatttctcc tatctctcat
     6421 tcctgtctca gatatctcct gtaacaggct gtgcgataaa gggcactccc ttgagaatga
     6481 tgttttcttc tctcgtttca gatgaaccca actttacagc tttctctgct tgttttcagc
     6541 aaccacaagc tctctgtgag agaaagaact gtaatttttc catctattat tttttagctt
     6601 ctagtaatct gcaatcgcag ctaggtcctt gcctccacga ccagagacat tgatgaagag
     6661 atgttcatct cggtacacct ttatactctt cgaaaatctc ttcaaaccgc gtcaacgtcg
     6721 ccttatcgta ggtatggtt
//
LOCUS       DAVLYV010000091         6718 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLYV010000091 DAVLYV010000000
VERSION     DAVLYV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.091"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..455
                     /locus_tag="VYO70_001836"
     CDS             <1..455
                     /locus_tag="VYO70_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1714020.1"
                     /translation="QGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISREEGLVDDIP
                     RLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHE
                     TLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH"
     gene            549..620
                     /locus_tag="VYO70_001837"
     tRNA            549..620
                     /locus_tag="VYO70_001837"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:582..584,aa:Glu,seq:ttc)
     gene            872..2417
                     /locus_tag="VYO70_001838"
     rRNA            872..2417
                     /locus_tag="VYO70_001838"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            2469..2541
                     /locus_tag="VYO70_001839"
     tRNA            2469..2541
                     /locus_tag="VYO70_001839"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2502..2504,aa:Ala,seq:tgc)
     gene            2666..5567
                     /locus_tag="VYO70_001840"
     rRNA            2666..5567
                     /locus_tag="VYO70_001840"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            5644..5759
                     /gene="rrf"
                     /locus_tag="VYO70_001841"
     rRNA            5644..5759
                     /gene="rrf"
                     /locus_tag="VYO70_001841"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            5764..5836
                     /locus_tag="VYO70_001842"
     tRNA            5764..5836
                     /locus_tag="VYO70_001842"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5797..5799,aa:Val,seq:tac)
     gene            5839..5911
                     /locus_tag="VYO70_001843"
     tRNA            5839..5911
                     /locus_tag="VYO70_001843"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5872..5874,aa:Asp,seq:gtc)
     gene            5936..6008
                     /locus_tag="VYO70_001844"
     tRNA            5936..6008
                     /locus_tag="VYO70_001844"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5969..5971,aa:Lys,seq:ttt)
     gene            6014..6095
                     /locus_tag="VYO70_001845"
     tRNA            6014..6095
                     /locus_tag="VYO70_001845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6048..6050,aa:Leu,seq:tag)
     gene            6114..6186
                     /locus_tag="VYO70_001846"
     tRNA            6114..6186
                     /locus_tag="VYO70_001846"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6147..6149,aa:Thr,seq:tgt)
     gene            6209..6280
                     /locus_tag="VYO70_001847"
     tRNA            6209..6280
                     /locus_tag="VYO70_001847"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6241..6243,aa:Gly,seq:gcc)
     gene            6288..6373
                     /locus_tag="VYO70_001848"
     tRNA            6288..6373
                     /locus_tag="VYO70_001848"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6322..6324,aa:Leu,seq:taa)
     gene            6383..6456
                     /locus_tag="VYO70_001849"
     tRNA            6383..6456
                     /locus_tag="VYO70_001849"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6417..6419,aa:Arg,seq:acg)
     gene            6472..6545
                     /locus_tag="VYO70_001850"
     tRNA            6472..6545
                     /locus_tag="VYO70_001850"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6506..6508,aa:Pro,seq:tgg)
     gene            6551..6624
                     /locus_tag="VYO70_001851"
     tRNA            6551..6624
                     /locus_tag="VYO70_001851"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6585..6587,aa:Met,seq:cat)
     gene            6638..6711
                     /locus_tag="VYO70_001852"
     tRNA            6638..6711
                     /locus_tag="VYO70_001852"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6672..6674,aa:Ile,seq:cat)
BASE COUNT         1892 a         1347 c         1930 g         1549 t
ORIGIN      
        1 tccaagggac tgtgtataag tgtagaaatg aatattacct tcatttatgg gaattgtcgg
       61 attgggacca agaaggcatg ctctgcttac atgaattgat tagtagagaa gaaggactgg
      121 tagacgatat tccacgttta aggaaatatt tcaaaaccaa gtttcgaaat cgaattttag
      181 actatatccg taagcaggaa agtcagaagc gtagatacga taaagaaccc tatgaagaag
      241 tgggtgagat cagtcatcgt ataagtgagg ggggtctctg gctagatgat tattatctct
      301 ttcatgaaac actaagagat tatagaaaca aacaaagtaa agagaaacaa gaagaactag
      361 aacgcgtctt aagcaatgaa cgatttcgag ggcgtcaaag agtattaaga gacttacgca
      421 ttgtgtttaa ggagtttact atccgtaccc actagtaagt catgcaaaaa aaatgaaaaa
      481 aattagaaaa agtagttgac aaagtttgaa aagactgtat aatagtaaga gttgaaaata
      541 acaactcagg tccgttggtc aaggggttaa gacaccgcct tttcacggcg gtaacacggg
      601 ttcgaatccc gtacggacta tggtatgttg cgtcaggagc acttgatgaa aaaaagttta
      661 aaaaaactta aaaatcttca aaaaagtgtt gacaattgaa agtggctgtg atatactaat
      721 atagttgtcg cttgagagaa gcaagtgaca aagacctttg aaaactgaac aagacgaacc
      781 aatgtgcagg gcgctacaac ataagttgta gtactgaaca atgaaaaaac aataaatctg
      841 tcagtgacag aaatgagtga gaactcaaac ttttaatgag agtttgatcc tggctcagga
      901 cgaacgctgg cggcgtgcct aatacatgca agtagaacgc tgaaggagga gcttgcttct
      961 ctggatgagt tgcgaacggg tgagtaacgc gtaggtaacc tgcctggtag cgggggataa
     1021 ctattggaaa cgatagctaa taccgcataa gagtggatgt tgcatgacat ttgcttaaaa
     1081 ggtgcacttg catcactacc agatggacct gcgttgtatt agctagttgg tggggtaacg
     1141 gctcaccaag gcgacgatac atagccgacc tgagagggtg atcggccaca ctgggactga
     1201 gacacggccc agactcctac gggaggcagc agtagggaat cttcggcaat ggacggaagt
     1261 ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga tcgtaaagct ctgttgtaag
     1321 agaagaatga gtgtgagagt ggaaagttca cactgtgacg gtatcttacc agaaagggac
     1381 ggctaactac gtgccagcag ccgcggtaat acgtaggtcc cgagcgttgt ccggatttat
     1441 tgggcgtaaa gcgagcgcag gcggttagat aagtctgaag ttaaaggctg tggcttaacc
     1501 atagtaggct ttggaaactg tttaacttga gtgcaagagg ggagagtgga attccatgtg
     1561 tagcggtgaa atgcgtagat atatggagga acaccggtgg cgaaagcggc tctctggctt
     1621 gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag gattagatac cctggtagtc
     1681 cacgctgtaa acgatgagtg ctaggtgtta gaccctttcc ggggtttagt gccgtagcta
     1741 acgcattaag cactccgcct ggggagtacg accgcaaggt tgaaactcaa aggaattgac
     1801 gggggcccgc acaagcggtg gagcatgtgg tttaattcga agcaacgcga agaaccttac
     1861 caggtcttga catccctctg accgctctag agatagagct ttccttcggg acagaggtga
     1921 caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg ttgggttaag tcccgcaacg
     1981 agcgcaaccc ctattgttag ttgccatcat ttagttgggc actctagcga gactgccggt
     2041 aataaaccgg aggaaggtgg ggatgacgtc aaatcatcat gccccttatg acctgggcta
     2101 cacacgtgct acaatggctg gtacaacgag tcgcaagccg gtgacggcaa gctaatctct
     2161 taaagccagt ctcagttcgg attgtaggct gcaactcgcc tacatgaagt cggaatcgct
     2221 agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg ggccttgtac acaccgcccg
     2281 tcacaccacg agagtttgta acacccgaag tcggtgaggt aaccgtaagg agccagccgc
     2341 ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg tagccgtatc ggaaggtgcg
     2401 gctggatcac ctcctttcta aggataagga actgcgcatt ggtcttgttt agtcttgaga
     2461 ggtcttgtgg ggccttagct cagctgggag agcgcctgct ttgcacgcag gaggtcagcg
     2521 gttcgatccc gctaggctcc attggtgaga gatcaccaag taatgcacat tgaaaattga
     2581 atatctatat caaatagtaa caagaaaata aaccgaaaac gctgtagtat taataagagt
     2641 ttatgactga aaggtcagaa aataaggtta agttaataag ggcgcacggt ggatgccttg
     2701 gcactaggag ccgacgaagg acgtgacaaa cgacgatatg ccttgggtag ctgtaagtaa
     2761 gcgatgatcc agggatttcc gaatggggga acccaacagg tagtacctgt tacccacatc
     2821 tgttaaggat gtgaggagga agacgcagtg aactgaaaca tctaagtagc tgcaggaaga
     2881 gaaagcaaaa gcgattgcct tagtagcggc gagcgaaacg gcaggagggc aaaccgaaga
     2941 gtttactctt cggggttgta ggactgcaat gtggactcaa agattataga agaatgattt
     3001 gggaagatca gccaaagaga gtaatagcct cgtatttaaa atagtctttg tacttagcag
     3061 tatcctgagt acggcgggac acgtgaaatc ccgtcggaat ctgggaggac catctcccaa
     3121 ccctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag gtgaaaagca
     3181 ccccgggagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt tcgagcccgt
     3241 taatgggtga gagcgtgcct tttgtagaat gaaccggcga gttacgttat gatgcgaggt
     3301 taagttgaag agacggagcc gtagggaaac cgagtctgaa tagggcgcct tagtatcatg
     3361 acgtagaccc gaaaccatgt gacctaccca tgagcaggtt gaaggtgcgg taagacgcac
     3421 tggaggaccg aaccagggca cgttgaaaag tgcttggatg acttgtgggt agcggagaaa
     3481 ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc tagcgtcgac
     3541 attagagatt cttggaggta gagcactgtt tgggtgaggg gtccatcccg gattaccaat
     3601 ctcagataaa ctccgaatgc caatgaatta tggtcggcag tcagactgcg agtgctaaga
     3661 tccgtagtcg aaagggaaac agcccagacc accagctaag gtcccaaaat aattgttaag
     3721 tggaaaagga tgtggggttg cacagacaac taggatgtta gcttagaagc agctattcat
     3781 tcaaagagtg cgtaatagct cactagtcga gtgaccctgc gccgaaaatg taccggggct
     3841 aaaacaattt accgaagctg tggatacctt tataggtatg gtaggagagc gttctatgtg
     3901 tgatgaaggt ataccgtgag gagtgctgga acgcatagaa gtgagaatgc cggtatgagt
     3961 agcgaaagac aggtgagaat cctgtccacc gtaagactaa ggtttccagg ggaaggctcg
     4021 tccgccctgg gttagtcggg acctaaggag agaccgaaag gtgtatccga tggacaacag
     4081 gttgatattc ctgtactaga gtatgtagtg atggagggac gcagtaggct aactaaagca
     4141 gacgattgga agagtctgtc taagcagtga ggtgtgaatt gagtcaaatg cttaattcta
     4201 taacattgag ctgtgatggg gagcgaagtt tagtagcgaa gttagtgacg tcacactgcc
     4261 aagaaaagct tctagcgttt aaacatactc tacccgtacc gcaaaccgac acaggtagtc
     4321 gaggcgagta gcctcaggtg agcgagagaa ctctcgttaa ggaactcggc aaaatgaccc
     4381 cgtaacttcg ggagaagggg tgctgacttt aagtcagccg cagtgaatag gcccaagcaa
     4441 ctgtttatca aaaacacagc tctctgctaa atcgtaagat gatgtatagg gggtgacgcc
     4501 tgcccggtgc tggaaggtta agaggagtgc ttagcgtaag cgaaggtatg aattgaagcc
     4561 ccagtaaacg gcggccgtaa ctataacggt cctaaggtag cgaaattcct tgtcgggtaa
     4621 gttccgaccc gcacgaaagg cgtaatgatt tgggcactgt ctcaacgaga gactcggtga
     4681 aattttagta cctgtgaaga tgcaggttac ccgcgacagg acggaaagac cccatggagc
     4741 tttactgcag tttgatattg agtgtctgta ccacatgtac aggataggta ggagtctaag
     4801 agatcgggac gccagtttcg aaggagacgc tgttgggata ctacccttgt gttatggcca
     4861 ctctaaccca gataggtgat ccctatcgga gacagtgtct gacgggcagt ttgactgggg
     4921 cggtcgcctc ctaaaaggta acggaggcgc ccaaaggttc cctcagaatg gttggaaatc
     4981 attcgcagag tgtaaaggta taagggagct tgactgcgag agctacaact cgagcaggga
     5041 cgaaagtcgg gcttagtgat ccggtggttc cgtatggaag ggccatcgct caacggataa
     5101 aagctaccct ggggataaca ggcttatctc ccccaagagt tcacatcgac ggggaggttt
     5161 ggcacctcga tgtcggctcg tcgcatcctg gggctgtagt cggtcccaag ggttgggctg
     5221 ttcgcccatt aaagcggcac gcgagctggg ttcagaacgt cgtgagacag ttcggtccct
     5281 atccgtcgcg ggcgtaggaa atttgagagg atctgctcct agtacgagag gaccagagtg
     5341 gacttaccgc tggtgtacca gttgtcttgc caaaggcatc gctgggtagc tatgtaggga
     5401 agggataaac gctgaaagca tctaagtgtg aaacccacct caagatgaga tttcccatga
     5461 ttatatatca gtaagagccc tgagagatga tcaggtagat aggttagaag tggaagtgtg
     5521 gcgacacatg tagcggacta atactaatag ctcgaggact tatccaaagt aactgagaat
     5581 atgaaagcga acggttttct tgatttgaat agatattcaa ttttgagtag gtattactca
     5641 gagttaagtg acgatagcct aggagataca cctgtaccca tgccgaacac agaagttaag
     5701 ccctagaacg ccggaagtag ttgggggttg ccccctgtga gatagggaag tcgcttagct
     5761 ttagggagtt tagctcagct gggagagcat ctgccttaca agcagagggt cagcggttcg
     5821 atcccgttaa ctcccaaagg tcccgtagtg tagcggttat cacgtcgccc tgtcacggcg
     5881 aagatcgcgg gttcgattcc cgtcgggacc gtttaaggta acgcaagtta ttttagactc
     5941 gttagctcag ttggtagagc aattgacttt taatcaatgg gtcactggtt cgagcccagt
     6001 acgggtcata tatgcgggtt tggcggaatt ggcagacgca ccagatttag gatctggcgc
     6061 ttaacggcgt gggggttcaa gtcccttaac ccgcattaag atataacaaa tgagccggct
     6121 tagctcagtt ggtagagcat ctgatttgta atcagagggt cgcgtgttca agtcatgtag
     6181 ccggcatttt tagatagaag aaaacagtgc gaacgtagtt cagtggtaga acaccacctt
     6241 gccaaggtgg gggtcgcggg ttcgaatccc gtcgttcgct tagagaggcc ggggtggcgg
     6301 aactggcaga cgcacaggac ttaaaatcct gcgattggta acgatcgtac cggttcgatt
     6361 ccggtcctcg gcataatatg gagcaccctt agctcaactg gatagagtac ctgactacga
     6421 atcaggcggt tagaggttcg actcctctag ggtgcatttt tctatttaac tcgggaagta
     6481 gctcagcttg gtagagtact tggtttggga ccaaggtgtc gcaggttcga atcctgtctt
     6541 cccgattcat ggcggtgtag ctcagctggc tagagcgtcc ggttcatacc cgggaggtcg
     6601 ggggttcgat ccccttcgcc gctataatga tcttgtcgga cctttagctc agctggttag
     6661 agctctcggc tcataaccga gtggtcgtag gttcaagtcc tacaaggtcc atttgaat
//
LOCUS       DAVLYV010000092         6221 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLYV010000092 DAVLYV010000000
VERSION     DAVLYV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.092"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..307
                     /locus_tag="VYO70_001853"
     CDS             <1..307
                     /locus_tag="VYO70_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714021.1"
                     /translation="SSWVEFKKAMYNERIAKFNNLISVSFNNPNVSWGRTNRITITSI
                     ANLQNMINTAVNEDAEDFRAQLYPDTNSRVLKLKKAIYKAYLDQTNDFRRSIFENKK"
     gene            351..5753
                     /locus_tag="VYO70_001854"
     CDS             351..5753
                     /locus_tag="VYO70_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1714022.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSIFLGSFLFALSVVGVSTYHYLDYS
                     TLTQTERDQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTVLGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDR
                     TVSSVSPASTVSPVEQPKVVTDKGEPEVQPALPEAVVTGKGKTEVQPTLPEAVVTEKG
                     EPEVQPVLPEAVVAEKGEPEVQPVLPEAVVTEKGEPEVQPALPEAVVTEKGEPEVHEK
                     PDYTQPIGANLVESEVHEKLAYTEPVGTTGVDENGNLIEPPVNDIPEYTEPISTVSEV
                     ASEREELPSLHTDIRTETIPKTTIEESDPSKFIGDNSVKQVGEDGERQIVTSYEELHG
                     KKISESVETVTILKEMKPEIIVKGTKERPKEKTAPVLTLTKVTEDAMNRSANLNYELD
                     NKDNAEISSIVAEIKDGDTVVKRVDLSKEKLTDAVQNLDLFKDYKIATTMIYDRGQGS
                     ETSKLDERPLRLELKKVEIKNIASTNLVKVNDDGTETPSDFMNEKPSEEDVKKMYLKI
                     TSRDNKVTRLTVDSIEEVTEEGQKLYKITAEAQDLIQHTDPTKVRNKYVYYIEKPHPK
                     EDNVYYNFKDLVDAMNTDKNGTFKLGADLNATGVPTPKKWYVDGDFRGTLKSVEGKHY
                     TIHNTERPLFQNIIGGTVTKVNLGNVNINMPWADRIAPIADTIKGGAKIEDVKVTGNV
                     LGRNWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFLTGIVGENWKGLVERAYVDAN
                     LIGKKAKAAGIAYWTQNSGDNHKVGVEGAVKKGIVKGTIQVESPVEVGGAVGRLSHHG
                     YIGEVVSMMKVKKGEIFYGSSDMNDDPYWVANNVRGNYVVNGVSEGTISYARAKEHHR
                     IKPISQSEADTKIMMLGITAQDFAVNEPVVNRLNRLTRKEDEYKSTQDYKVDRDLAYR
                     NIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGMKDGQFVTDLSDIDKIMVHYA
                     DGTKEEMDVTKNADSKVKQVREYTIAGQNVVYTPNMVEKDRNQLIQDIKDKLASVQLI
                     SPEVRALMDARKKPEENTDERKNGYIKDLYLEESFAETKANLDKLVKSLVENADHQLN
                     SDEAAMKALVKKVDENKAKIMMALTYLNRYYDIKYGDMTIKNLMMFKPDFYGKSVDLL
                     DFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGFLDYNMRLFTNDTDLNDWFIH
                     AAKNVYVSEPQTTNPDFVNKRHRAFDGLNNGVHNRMILPLLTLKNAHMFLISTYNTMA
                     YSSFEKYGKYTEEARNEFKKEIDKVAHAQQTYLDFWSRLALPSVRDQLLKSENRVPTP
                     VWDNQNYSGIKGINRMGYDEKKVPIAPIRELYGPTWKFHNTNWNMGAMASIFPNPNNN
                     DQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRHRQGTDVEAYAQGMLQTPSSI
                     GHQGEYGALGLNMAYHRENDGDQWYNYDPDKLQTREDIDRYMKNYNEALMMLDHVEAD
                     AVLPQLNGDNSKWFKKIDREMRRNLGDGLNNLVAPHQWDNVRDLNQEESSKKLSSIND
                     LIDNNFMTKHGNPGNGRYRPEDFRPNSAYVNVNMMAGIYGGNTSQGAPGSLSFKHNAF
                     RMWGYYGYDKGFTSYVSSKYQGEADKQNQGRLGDDFIIKKVSNNQFSNLEDWKKYWYH
                     DVKSRAEKGFTEITIDGQTIHNYNELKDLFDKAVTEDLKKAGNYSNTENLKSKVFKAL
                     LKNTDGFFNPLFKKDI"
     gene            5920..>6221
                     /locus_tag="VYO70_001855"
     CDS             5920..>6221
                     /locus_tag="VYO70_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1714023.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADAYFPKLLEGG"
BASE COUNT         2199 a          985 c         1280 g         1757 t
ORIGIN      
        1 ctcatcttgg gtagaattta aaaaagcaat gtataatgag cgaatagcta aatttaataa
       61 tctaataagt gttagcttta ataatccaaa tgtaagttgg ggaagaacta atagaataac
      121 aataactagc atagccaacc ttcaaaatat gataaatacg gctgtcaatg aagatgctga
      181 agattttaga gcacaattat accctgatac aaatagtaga gttcttaaac tgaaaaaagc
      241 aatatataaa gcctatcttg accaaacaaa tgattttaga agatcaattt ttgagaataa
      301 aaaatagtgt ttactattag gaaataaaaa gtgaatgtgg aggattagaa ttgtcattat
      361 taaaaaaaga taaattttcc attaggaaaa taaagggtat tgttggttcg atattcttgg
      421 ggagtttttt atttgcactg tcagttgttg gtgtatcgac ttatcattac ttagattata
      481 gtactttgac acaaactgaa cgtgatcaac tcaaacaagg tagacctgat gaatcaaaag
      541 agtcatatgc tttggtttat gaaaaagacg cattaccaaa cacaggaagt tctcaatcta
      601 ttatgactgt attgggtttg ttagctatcg ggagtcttat tgtgattatc acaaaagata
      661 aaaagaggaa aaaaatagct acatttttga ttgtaggggc gacaggttta gttacactat
      721 caactgcttc agcacttaat ttgaatgcta acatccatga gtctggacgt gatggtgtgt
      781 tgcaaatttc tggttacaga tatgttggtt acttggagct tgatgataga actgtttcgt
      841 cggtttctcc agcatcaact gtttctccag ttgagcaacc caaagttgtg actgacaaag
      901 gcgaaccaga agttcagcca gcgttaccag aagcagttgt aactggaaaa gggaaaactg
      961 aagtacaacc gacattacca gaagctgttg tgactgaaaa aggggaaccg gaagtacaac
     1021 cagtgttacc agaagctgtt gtggctgaaa aaggggaacc ggaagtacaa ccagtgttac
     1081 cagaagctgt tgtgactgaa aaaggggaac cagaagtaca accagcctta cccgaagcag
     1141 ttgtaactga aaaaggcgaa ccagaagttc atgagaagcc tgactatacg caaccaatag
     1201 gtgcaaacct tgttgagtct gaagttcatg agaaactagc atacactgaa cctgtaggta
     1261 caacaggagt agatgaaaat ggaaacttga ttgagccacc tgttaacgat attccagagt
     1321 atactgaacc aatatctaca gtttcagaag ttgcatcaga aagagaagag ttgccttctt
     1381 tacataccga tattcgtaca gagactattc ctaaaaccac tatagaagaa tctgatccta
     1441 gcaaatttat aggcgataat tctgttaaac aagttggtga ggatggcgaa cgtcaaattg
     1501 ttactagcta tgaagagtta catggcaaaa aaatcagcga gtcagttgaa acagtaacaa
     1561 ttttgaaaga aatgaagcct gaaattattg taaaaggtac aaaagaaaga ccaaaagaaa
     1621 aaacggctcc tgttttaacc ttgaccaaag taacagaaga tgctatgaat cgttctgcca
     1681 acttaaatta tgagcttgat aataaagata atgctgaaat atcaagtatt gtagctgaaa
     1741 ttaaagatgg tgatacagta gttaaaaggg ttgatttatc taaagaaaaa ttaactgacg
     1801 ctgtacaaaa tttggatttg tttaaagatt ataagattgc aacaacaatg atttatgatc
     1861 gtggtcaagg ttctgaaacg tctaaactgg atgaaagacc tctgcgtttg gagttgaaaa
     1921 aggtcgaaat taaaaatatc gcatctacta atttagtgaa ggtaaatgat gatggtactg
     1981 aaacacctag tgattttatg aatgaaaaac cttcagaaga agatgttaaa aaaatgtact
     2041 taaaaatcac tagtcgcgat aataaggtaa ctcgtttaac tgtagactct attgaagaag
     2101 tgactgaaga aggtcaaaaa ctttacaaaa taactgctga agcacaagat ttgattcaac
     2161 acacagaccc aacgaaggtt cgtaataagt atgtttatta tatagaaaaa ccccatccta
     2221 aagaagataa tgtctactat aactttaaag atttagtaga tgctatgaat actgataaaa
     2281 atggtacatt taaacttggt gcagatttga atgccactgg tgttccaaca cctaagaaat
     2341 ggtatgttga cggcgacttt agaggtacat tgaagagtgt agaggggaaa cattatacta
     2401 tccacaacac ggagcgtccg ttattccaga atattattgg tggtacagta accaaagtaa
     2461 atctgggaaa tgtaaatatc aatatgccat gggcagaccg catcgcaccg attgctgaca
     2521 ctattaaagg tggcgctaaa attgaagatg tcaaagtgac tggtaacgta ttaggtagaa
     2581 actgggtatc tggatttatt gataagattg acaaccaagg tacactcaga aatgtagcct
     2641 ttatcggaaa tgtgacatct gtaggtgatg gtggtcaatt cttgaccggt atcgttggtg
     2701 agaactggaa aggactagtc gaacgtgcgt atgttgacgc aaatcttatt ggcaagaaag
     2761 ccaaggctgc tggtattgct tattggacac aaaacagtgg tgacaatcat aaagttggtg
     2821 tcgaaggtgc agtcaagaaa ggaattgtga agggtaccat tcaggtagaa agtcctgttg
     2881 aagtcggcgg tgctgttgga cgtttgagtc atcatggtta tatcggggaa gtcgtatcta
     2941 tgatgaaagt taagaagggt gaaatttttt atggttctag tgatatgaat gatgacccat
     3001 attgggtggc gaataatgta cgaggaaatt atgtagttaa tggtgtgagt gaaggtacga
     3061 tatcttatgc tcgtgctaaa gaacaccatc gtatcaaacc aatttcacag tcagaagcag
     3121 atactaaaat tatgatgtta ggtattacag cacaagattt tgctgtcaac gaacctgtgg
     3181 tcaatcgctt aaaccgttta accagaaaag aagatgaata taagtcaacc caagactata
     3241 aagtagatcg agatctggct tatcgtaata ttgagaaact tcaaccattt tataacaaag
     3301 aatggattgt caaccaaggt aacaaacttg cagaagattc aaacttagct aagaaagaag
     3361 tcctttctgt tacgggtatg aaggatggac agtttgtaac tgacttatca gatattgata
     3421 agattatggt tcactatgct gatggtacta aagaagaaat ggacgtcact aagaatgctg
     3481 attctaaagt gaaacaagtt cgtgaatata ccatcgctgg acaaaacgtt gtttatacac
     3541 caaatatggt tgagaaagac cgtaaccaat tgattcaaga tatcaaagat aagttggcgt
     3601 cagttcaatt gatttctcct gaagtacgtg ctttgatgga tgcacgtaag aaaccagaag
     3661 aaaatactga tgaacgtaag aatggttata ttaaagacct ttacttagaa gaaagctttg
     3721 ctgaaacgaa agctaatctt gataaattgg ttaaatcctt agttgaaaat gcagatcatc
     3781 aattaaatag tgacgaagcg gctatgaaag cacttgttaa gaaggtggat gaaaataaag
     3841 ctaaaatcat gatggctctg acttatctca atcgttacta tgatatcaag tatggggata
     3901 tgactatcaa gaacttgatg atgtttaaac ctgatttcta tggtaaatca gttgaccttc
     3961 ttgatttctt gattcgaatt ggatcaagtg agagaaatat caaaggtgac agaactttgg
     4021 atgcttatcg tgatatgatt ggtggaacta ttggtaagtc tgaattacat ggattcttag
     4081 attacaatat gcgtctcttc actaacgata ctgatttaaa tgattggttc atccatgcag
     4141 ctaagaatgt atatgtatca gaacctcaaa caacgaaccc agattttgtt aataaacgac
     4201 atcgtgcatt tgatggtttg aacaacggtg ttcataatcg tatgattcta cctctattaa
     4261 ctcttaagaa tgcacatatg ttcttgattt caacttacaa tacaatggct tatagttctt
     4321 ttgagaaata tggtaagtat acagaagaag ctagaaatga attcaaaaaa gaaattgata
     4381 aggttgcaca tgctcaacaa acatacttag acttctggtc acgtttagca ttgccaagcg
     4441 tacgtgacca attactcaag agtgagaata gggtaccaac tcctgtgtgg gataaccaaa
     4501 actatagtgg tattaaagga ataaaccgta tgggttatga tgagaagaaa gtaccaattg
     4561 cgccaattcg tgagttatat ggtccaactt ggaaattcca taataccaac tggaatatgg
     4621 gagctatggc atctattttc ccaaatccta ataacaatga ccaagtttat ttcatgggaa
     4681 caaatatgat tagtccgttt ggtatttcag cctttactca tgaaactaca cacgttaatg
     4741 atagaatgct ttacttcggt ggtcatagac accgtcaagg tacagacgtc gaagcctatg
     4801 ctcaaggtat gctacaaact ccgtcaagta ttggtcacca aggtgaatat ggtgctttag
     4861 gtttgaacat ggcttatcat cgtgaaaatg atggtgacca atggtataac tatgaccctg
     4921 ataagcttca aactcgtgaa gatatcgatc gatatatgaa aaactataat gaagcactga
     4981 tgatgctaga tcatgtagaa gcagacgccg tacttccaca attgaatggt gataatagca
     5041 agtggttcaa gaaaattgat agagaaatgc gcagaaatct tggggatggt ttgaataact
     5101 tagtagcacc gcaccaatgg gacaacgttc gtgacttaaa tcaagaagag agttctaaga
     5161 aattatcaag tattaacgat ttgattgata ataatttcat gaccaaacac ggaaatccag
     5221 gtaacggacg ttatagacca gaggatttta gaccaaactc agcttacgta aatgtcaata
     5281 tgatggcagg aatttacggt gggaatacga gtcaaggtgc tcctggttca ttatcgttca
     5341 aacataatgc cttccgtatg tggggttatt atggatatga caaaggtttc actagttatg
     5401 tatctagcaa atatcaaggt gaagctgata aacaaaacca gggtagatta ggtgatgatt
     5461 tcattatcaa aaaagtttca aacaaccaat tctcaaatct tgaagattgg aagaaatatt
     5521 ggtatcatga tgtgaaatcc agagctgaaa aaggattcac agagattaca attgatggac
     5581 aaaccattca caactataat gaactgaaag acttattcga caaagctgtc acagaagacc
     5641 tcaagaaagc tggaaactac tcaaatactg agaatttgaa atccaaagtc ttcaaagctc
     5701 ttcttaaaaa cacagacggt ttctttaatc cattgttcaa aaaagacatt taaaccccaa
     5761 ttattcaccc ctaatctaaa aaccatccag aatccttgcc ttagcttaga tcctggatgg
     5821 tttctttttt cacccaatgg gtgtttttta ctagacaaaa aagagtttcc cctttatggt
     5881 ataagtgtag aaaaaaacac aaaaagaaag gaaactcaca tgaacagttt accaaatcat
     5941 cacttccaaa acaagtcttt ttaccaacta tctttcgatg gaggtcattt aacccagtat
     6001 ggtggtctta tcttttttca ggaacttttt tcccagttga aactaaaaga gcggatttct
     6061 aagtatttag taacgaatga ccaacgccgc tactgtcgtt attcggattc agatatcctt
     6121 gtccagttcc tctttcaact gttaacaggc tatggaacgg actatgcttg taaagaattg
     6181 tcagctgatg cctactttcc aaaattgttg gaaggagggc a
//
LOCUS       DAVLYV010000093         6126 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLYV010000093 DAVLYV010000000
VERSION     DAVLYV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.093"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            152..460
                     /locus_tag="VYO70_001856"
                     /pseudo
     CDS             152..460
                     /locus_tag="VYO70_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            624..1283
                     /locus_tag="VYO70_001857"
     CDS             624..1283
                     /locus_tag="VYO70_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1714024.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            1308..2375
                     /locus_tag="VYO70_001858"
     CDS             1308..2375
                     /locus_tag="VYO70_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1714025.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            2479..3489
                     /locus_tag="VYO70_001859"
     CDS             2479..3489
                     /locus_tag="VYO70_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714026.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFIIPSDVL
                     QF"
     gene            3501..5192
                     /locus_tag="VYO70_001860"
     CDS             3501..5192
                     /locus_tag="VYO70_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1714027.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            5195..5905
                     /locus_tag="VYO70_001861"
     CDS             5195..5905
                     /locus_tag="VYO70_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1714028.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2051 a          840 c         1169 g         2066 t
ORIGIN      
        1 tatcgcttac caatatttgc taaactttca gaaaaaaagt tggagtatat gatgctaggt
       61 gactgcgtca tgttagtaaa cgaaatggaa ataactgatc atagagtaga taacttattt
      121 gaaaaaggga aaaatgagat aaaagattca agtagtagat ttagcaatag gacatataaa
      181 agctttagaa aaagtatctg aaaaaacaga tgtttatatc tataacctag gctcgggaga
      241 aggcactagt gtattacaac ttgtaaatac atttgaaagt gttaataaga tccctattcc
      301 ttataaaata gttccaagac gttcaggaga cgttgcaact tattatgcaa atgcagacaa
      361 agcatataag gaattaaatt ggaggacaac aaaatcgatt gaagacatgt gtagagatac
      421 atggaattgg caatcaaaaa atcccaatgg ctataattga tgatactgtc tatatgagtg
      481 ctgtattttg gaaaaattac ttaacctttt aatgaaagat aaattaaaaa aaatgtttga
      541 cagcgcttga caaaggaaac ggtttcattg tataatgtaa acacaatgac accgatgtca
      601 aatataaaaa agaaggtctt tatatgaaaa acttaacaaa aattaagttc aaagaaaatg
      661 gggaatttaa tcatttccct gggaatacag ttgtagcaaa tctttatact aaacaagatt
      721 tgatggaagt tgttgatatt attcaatcac gttatagaga attaccattt atcgataagt
      781 ttactttaac tccaaggaat tctattcaca tgactgtaat tgaattgttg tgccatgaaa
      841 atcgtgaaac ggagttttgg agcagtaatc ttcctctaga tacaccttta caggaaatac
      901 atgattactt tgctaaacaa cttgaaattt ttccattgtt ggatgaagaa attcatatgc
      961 gtataactga aatgggaaaa caaaacatac tagttgaacc tgcagatgaa gcttctgcaa
     1021 agagattaga agaaattcgt acttatgtct cagaaaaagc aggtgtttgt ttccctaatc
     1081 atgatagata tcaattccat atttcaattg ggtatcttcg gattcctcta accgaagagg
     1141 aggaagaaga gtttactaaa gtcagagcag aattaactga aattttatta gagaagattc
     1201 caactattac tgtaaaccgt attgactata ctgtatttga agatatgaga caatttgttc
     1261 catatcacga aaaatttaaa taaaataata aaaaaggaga acttatcatg aaaaaaatga
     1321 aagtttggtc tactgtactt gcaacgggag ttgctcttac tacacttgct gcttgctctg
     1381 gaggttcaaa ttctacgact gcttcttcat ctgaagaaaa agctgataaa agtcaagaat
     1441 tagttatcta ttcgaactca gtctcaaatg gtcgtggtga ttggttaact gctaaagcaa
     1501 aagaagctgg ttttaatata aaaatggttg atatcgctgg cgctcaatta gcagaccgtg
     1561 ttattgctga gaagaataat gcagttgcag atatggtatt tggaattggt gctgttgatt
     1621 caaataaaat tagagatcaa aaattactag tacagtacaa gcctaaatgg ttagataaaa
     1681 ttgatcaatc tttatcagat aaagataatt attataatcc tgtgattgtt caaccattag
     1741 ttttaattgg ggcgcctgat gtaaaagaaa tgcctaaaga ttggactgaa ttaggtagta
     1801 agtataaagg taaatattca atttatggtc ttcaaggtgg tacaggacgg gcaattctag
     1861 caagtatctt agttcgttac cttgatgaaa aaggagattt aggcgtatca gaaaaaggat
     1921 gggaagttgc aaaagaatac tttgcaaatg cctatactct tcaaaaaggt gaaagctcag
     1981 ttgttaaagc gttagataaa gaaagcccaa ttcaatacgg tatgatctgg ggttctggag
     2041 cgttaattgg acaaaaagaa caaaatgttg aattcaaagt tatgactcct gagattggtg
     2101 taccatttgt aactgaacaa actatggttt taagcactag taaaaaacaa gcgttagcta
     2161 aagaatttat tgattggttt ggtcaatcag aaattcaagt agaatatagt aaaaaatttg
     2221 gatctattcc tgccaataaa gatgccctaa aagagttacc tgaagatacg aaaaaatttg
     2281 ttgatcaagt gaaaccacaa aatattgact gggaagctgt tggaaagcat ttggatgaat
     2341 gggtagaaaa agctgaatta gaatacgtaa aataagaaat aagagttatt ttttatgtcc
     2401 aagtctctct attatttcta aatttaagga agaaataaag agaggctttt ggttttagta
     2461 atagagggag ttagtgagat gattaaattt gataatattc aaattaaata tggtgatttt
     2521 gttgcaattg ataatctgaa tttagatata catgaagggg aattctttac atttcttggg
     2581 ccttcaggat gtggtaaatc aactactttg agagcattgg taggtttttt agatccatca
     2641 tcaggaagta ttgaagttaa tggaacagat gtcactcatt tggaacctga aaagcgtgga
     2701 attggtattg tatttcaatc ttatgcgcta tttccaacta tgactgtttt tgataatatt
     2761 gcatttggtt taaaagttaa gaaggtagct ccagatgtta ttaaagctaa agtatcagca
     2821 gtggcagcaa aaattaagat ctctgatcaa cagttacagc gtaatgtatc agaattatct
     2881 gggggtcaac aacaaagggt agcattggct cgtgctctgg ttcttgaacc taaaattctt
     2941 tgtctagatg aaccattgtc aaaccttgac gcaaaattac gtgtagattt gagaaaagag
     3001 ttgaaaagac ttcaaaaaga gttaggtatt actactttat atgttactca tgatcaagag
     3061 gaagccttga ctttatctga tagaattgca gtctttaaca atggatacat cgaacaggtc
     3121 ggtacaccag tagagattta tcataattct caaactgaat ttgtatgtga ttttattgga
     3181 gatattaatg ttttgaccga tgaaacagtc cacgaagtat tattgaaaaa tacaagcgtt
     3241 ttcttagagg ataaaaaagg atacattcga ttagagaaag ttcgattcaa tcgtgaaact
     3301 gaacaagatt ttattctaaa agggacaatt attgatgttg agttttctgg agttacaatt
     3361 cactatacaa taaaagtttc tgaaagtcag attcttaatg taacaagtat tgatagtcag
     3421 gctgctatta gatctgtcgg agaaagtgtg gaattattta tcataccatc agacgttctg
     3481 caattttaag gaggtgcagt atgcgtcata aattaaattt aaaagattgg cttattcgtt
     3541 tagggttaat ctggttctta gtaacattta ttatttatcc aaactttgat ctagtagtga
     3601 atgtatttgt aaaaggagga gaattttccc ttgatgctgt acatcgtgtt ctaaaatctc
     3661 agagggcact tcagagtatt atgaacagtt ttaagttagc attttcactc attattacag
     3721 ttaatgtcgt aggtattctt tgtgttctat ttacagagta ctttgatatt aaaggtgcta
     3781 aaattttaaa attaggttat atgacctctt taatttatgg aggagtggtt ttagcgactg
     3841 gatataaatt tgtctatggt ccttatggat tgattacaaa atttttacaa aatgttatcc
     3901 cttctttaga ctctaactgg tttattgggt atggtgcagt cttattcatt atgacatttt
     3961 caggaactgc taatcataca ttgtttttaa caaatacaat tcgaagcgtt gactatcaca
     4021 ctattgaggc tgctcgaaat atgggagcaa aaccatttac tgttttccga aaagtagtgt
     4081 taccaacctt aattccaact ctatttgcac ttactattat ggtttttctt agtggtttat
     4141 ctgcagtagc agcacccatg attgttggtg gtaaagaatt tcaaactata aatccaatga
     4201 ttattacatt tgcagggatg gggaattctc gtgatttagc tgccttactt gcaattattt
     4261 taggtattgc aactacaatt ttgcttacta tcatgaataa gatagaaaaa ggtggaaatt
     4321 atatttctat ctctaagact aaagcgcctc ttaaaaaaca aaaaattgcg tctaagcctt
     4381 ggaatatcat tgctcacatt gtagcatatg gattgttcac agttttcatg cttccactaa
     4441 tttttatagt attatactca tttacagatc cagttgcaat tcaaacaggt aacttaacat
     4501 tatcaaactt tactttagaa aattatcgct tattctttag taatagtgcg gcattctctc
     4561 cattcttggt cagctttatt tattctatta ttgctgcgac aacagcaaca attctcgcag
     4621 ttgtatttgc tcgtgttgtc agaaaacata aatctcgttt tgatttctta tttgaatatg
     4681 gtgctctact tccttggtta ctaccaagta cacttttagc agtaagttta ttatttactt
     4741 ttaatcagcc acaatttctt gttttgaatc agattttggt aggtagtttg gtaattctac
     4801 ttattgcata tatagttgta aaaatcccat tttcttatag aatggtacgt gctattttat
     4861 ttagtgttga tgatgagatg gaagatgcag caagaagtat gggtgcttca cctttttata
     4921 ctatgatgaa ggttatcatt ccatttattt taccggttgt tctctctgtt attgctttaa
     4981 actttaactc tttattaact gacttcgact tatctgtatt cctttaccat cccctagctc
     5041 aaccattagg tattacgatt cgatctgcag gtgatgaaac agcaacatct aatgcacaag
     5101 ctctggtatt tgtttataca attgttctga tgattatttc tggaacggta ttatacttca
     5161 cacaaagacc ggggcgtaaa gtaaggaaat aatcatgaca gccactagtc ttgggttatc
     5221 aaatattgaa atagttgtca ggattgtttt atcagtagtc attggtagta taattggttt
     5281 agagagaggg agcaaatccc agcctgcagg catccgaact tatagtattg tttgtctagc
     5341 tgcatgtttg attatgatga cgaatgaata cgtatcttat aaatttggga caggagatcc
     5401 tacacgatta ggagctcaag ttatatcagg tgtgggtttt ctaggcgctg gaacgattct
     5461 tattacagat aaaaagaaaa ttacaggtct gacaactgca gcaggcattt gggcttcggc
     5521 aggaattgga ttagctattg gagtaggttt ttatgaggga gctcttttag tagccatttc
     5581 tgtttggggt gtgatatcca tgttccaacc actaaaaaaa tatctgcaaa atcgttctaa
     5641 aatgattgaa ttgtatatag tggttaaatc tacagaagcc tataatcgtg tattggttta
     5701 ttgtgcagaa aatggtatca gaatgaccga ttcaagaaca gcgtttggag atgttaattc
     5761 tgacagaatt gaatactttg atgttccgga caaaaaaata gcgtcattta ttactctgga
     5821 attatcaggt agatttgaac atcttcggct gatggaagaa attgctaata ttgtaggtgt
     5881 tatttatgtc gaagaaatta gctgaaattt agtaaacaaa gggacagatg tttttgtcct
     5941 tttgcttttt agatgtttta atcatctaat ttttgagtag atttttgctc agaggttttt
     6001 atgaaagatt ataaaatcaa ttttgattta ggtaaaatag aatattttga taataattgt
     6061 ttgatacaag tatacaaatt tatttctttt tatgatattt gtgaaatggt gtttgccttt
     6121 cattta
//
LOCUS       DAVLYV010000094         6090 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLYV010000094 DAVLYV010000000
VERSION     DAVLYV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6090
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.094"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            69..311
                     /locus_tag="VYO70_001862"
     CDS             69..311
                     /locus_tag="VYO70_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1714029.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            313..567
                     /locus_tag="VYO70_001863"
     CDS             313..567
                     /locus_tag="VYO70_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1714030.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            614..1309
                     /locus_tag="VYO70_001864"
     CDS             614..1309
                     /locus_tag="VYO70_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEV1714031.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMTDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENIFDLLTILPLNAIFTVFRLGRIFRLARLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPVSLFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSS"
     gene            complement(1646..2632)
                     /locus_tag="VYO70_001865"
     CDS             complement(1646..2632)
                     /locus_tag="VYO70_001865"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1714032.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            2826..5294
                     /gene="gyrA"
                     /locus_tag="VYO70_001866"
     CDS             2826..5294
                     /gene="gyrA"
                     /locus_tag="VYO70_001866"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1714033.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQILSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            5294..6037
                     /gene="srtA"
                     /locus_tag="VYO70_001867"
     CDS             5294..6037
                     /gene="srtA"
                     /locus_tag="VYO70_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1714034.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWNAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
BASE COUNT         1927 a         1007 c         1317 g         1839 t
ORIGIN      
        1 tttttatatt gactgaacac ctaaaaagta atacaatggt gttaccatta aaaaagggag
       61 cacaaaagat gactactatt acattaaaag tttctgaagc tgataaaaca tttatgaaag
      121 caatggctaa gtttgaagga gtttcccttt cggaacttat tcgcaccaaa actcttgaag
      181 ccctagaaga tgaatacgat gctcgtgtgg cagatttagc ctatcaagag tatttagaag
      241 acttggaaaa aggagttgaa cccattactt gggaagaaat gatgcatgat ttaggcttga
      301 aggatgaata atttgtataa attagttcca acaagacgtt ttatcaagca attgaaaaaa
      361 ttggaccgtt atacgcagaa gctaattaca aactatttac aaaccaatgt tttggaagac
      421 ccaagacgac acggaaaggc tttggttggt aatcgcgttg gtcaatggcg ctatagaatt
      481 ggtaattatc gagttatcgt acaaattgta gatgatgaat tagtcgttgc tactctagaa
      541 gttggtcatc ggagagatat ttattgaatt aaagctgagt aataacagta tagaaagtaa
      601 ggagagtaaa aatttgaaga aaaaatggtt ttttgcagat tattatgaca caacaattat
      661 tttacttgct ttaatctctg ttattctggt attacttgga tttgctgaga tgactgattt
      721 ggacaatccg ccttatagta ttattgattt agtaatctgg ggtgtttttg taattgatta
      781 tagttggcgc ttttttatta ctaaaagaaa atggcgcttt attcttgaaa atatttttga
      841 tttattgacc attctccctt taaatgctat ttttacagtg tttcgattag gacgtatctt
      901 ccgcttagca aggctgacaa aattactgaa actgactcgt ttacttagaa taattggact
      961 tacaggtaaa ctagaaagaa aaattagtag attcttacga acaaatggat tgatttatat
     1021 cttgtatgtt aatatcttta ttgttctagt agggagttcg attttatccg ttgttgaaga
     1081 aaaatccttc tcagatagtc tttggtgggc tcttgtgaca gtaactactg ttggttatgg
     1141 tgatattgtt ccagtttccc tttttgggaa atggctagca gttttactga tgcttgtcgg
     1201 tattggaaca atagggatgc taacaagtgc tttgacgaac ttttttgtaa aggataatcc
     1261 agatgaacag ataaaacttg ataaactcaa agacgaatta tcgagctaaa gaatattaat
     1321 agagaaacaa tctgaaaaaa tcgaagaact acattgaatg ataaaagact tagttgaaaa
     1381 aatatcataa aggtgagaca aaataaaact tgattcgagg agaaatcaag taagtcttga
     1441 cacaatagaa cgcatattat caggtataaa aacttgatac tatgcgtttt atagagaaat
     1501 aaaataaaat ctgaacaaat caattaggaa tttaaagcat tttataacaa cgttttagag
     1561 tgatggggtg ctatttcaac ttcaacctac tataatacag aaaaaaacaa ctccctgatg
     1621 attcaaggag ttgtctatag ttaaattagt ttttagaagc ttcttggaat tctggatttt
     1681 tccatgcttc gtcaataata gcttgcaatt ctttagcaga tgcttgcatt ttttgagttt
     1741 ctgcatcgtt caatggaatg tttactggac gaacgatacc atgtgcacca acaacagctg
     1801 gttgaccgat aaagacattc tcaactccgt attgaccttc ttggaatact gaaagtggaa
     1861 gtactgcgtt ttcatcgtca aggattgctt tagtgatacg agcaagggct actgcgatac
     1921 cgtagtatgt tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga acaccttcga
     1981 acaattcaat caattcagct tcttgaacat tttgagtgtc tttaaggaat tcttcaaggt
     2041 ttacaccagc gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg tgttcaccca
     2101 tgatgtaggc gtgcactgaa cgagcatcca catccaattt ttcagcaagt gcttgacgga
     2161 aacgagctga gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg aaaccagaga
     2221 atttccaagt tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg aagatacctt
     2281 tgaaaccaga ttcaacaact tgagttacga ttgatttgtt gatagcaagg tttttaccta
     2341 caaggtcaag acgagtttca cctggttttt gaggtgcacc tgcagtgatc acaacaaggt
     2401 cagcgtctgc acagtcagag tattgagctg catagatttt tttaggtgaa gtgaaggcaa
     2461 gggcgtgact aaggtcaagc gcatcaccaa cagctttttc atgcaattgt ggaatttcga
     2521 taattccaag ctcttgtgca attccttggt taacaagtgc aaaagcgtaa gatgaaccta
     2581 cagcaccatc accgacaagg ataacttttt tgtgttgttt agttgaagtc attgttttaa
     2641 acatctcctt aattttatta ggggattttc cctagacaac ttcattctat cacttttaaa
     2701 aaactttgtc acgaatatgc cttatagttc tcgatgtaaa cgttttagtg gtttagaggc
     2761 tgaaatagat gggaatttat ggtataatgt tgttacttac taattgtgaa atgaggcatt
     2821 tattaatgca ggataaaaat ttagtgaatg tcaatctgac aaaggagatg aaggcaagtt
     2881 ttatcgacta cgccatgagt gttatcgtag cgcgagctct tcctgatgtt cgagatggct
     2941 taaaacctgt tcaccgtcgc attctctacg gaatgaatga attgggtgtg accccagaca
     3001 aaccccataa aaaatctgct cgtattacag gggatgtcat gggtaaatat cacccacacg
     3061 gggattcctc tatttatgaa gccatggtcc gtatggctca atggtggagc taccgttaca
     3121 tgcttgtaga tggtcatggg aattttggtt ccatggatgg agatagtgct gccgctcaac
     3181 gttataccga ggcacgtatg agcaagattg ctctggaaat gcttcgtgat atcaacaaaa
     3241 atacagttga tttcgttgat aactatgatg ccaatgaacg ggaacccttg gtcttgccag
     3301 cgcgttttcc aaaccttttg gttaatggag caactggtat cgcggttggg atggcaacca
     3361 atattccacc tcataatctg ggtgaaacca ttgatgcagt gaagttggtc atggataatc
     3421 ctgaagtgac taccaaggac ttgatggaag tcttgcctgg accagatttt ccaactggtg
     3481 ctcttgtcat ggggaaatca ggtatccata aggcttatga aacaggtaaa ggttcgattg
     3541 tcctacgttc tcgtacagag attgaaacga ctaagactgg tcgtgagcgc atcgttgtaa
     3601 cagaatttcc ttacatggtc aataaaacca aggtgcatga gcatattgtt cgcttggttc
     3661 aggaaaaacg cattgagggt atcacagcag tacgtgatga gtcaaaccgt gaaggtgttc
     3721 gatttgttat tgaagtcaag cgcgacgcct cagccaatgt tattctcaat aacctcttca
     3781 aaatgaccca aatgcaaacc aattttggtt tcaatatgct cgctatccaa aatggtatac
     3841 cgaaaatttt gtctcttcgt cagattttgg atgcttatat cgagcaccaa aaagaagtgg
     3901 ttgttcgtcg tacacgtttt gataaggaaa aagcggaagc gcgcgctcat atcttagaag
     3961 gtctcttgat tgcgctagac catatcgacg aagtgattcg tatcatccgt gctagtgaaa
     4021 cggatgcgga agctcaagct gagttgatga gcaagtttaa gctttctgaa cgtcaaagtc
     4081 aagctatcct tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat aagattcaat
     4141 ctgagtatga tgacctcttg gctctgattg cggatttagc agatattctt gctaagcctg
     4201 aacgtgtttc tcaaattatc aaagacgaat tggatgaagt taaacgtaaa ttttctgata
     4261 aacgccgtac agagttgatg gttggacaga tcttgagtct cgaggatgag gacttgattg
     4321 aagaatcgga tgtcttgatt accctttcta acagaggcta cattaagcgt ttggatcagg
     4381 acgagttcac tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga gtgaaagatg
     4441 atgactttgt tcgtgagtta gtgtcaacta gcacacatga tcatctgctc ttcttcacaa
     4501 acaagggacg tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt cggactgcca
     4561 aagggctacc agtagtcaat ctcttgaaat tggatgaaga cgaaagtatt cagacggtta
     4621 tcaatgttga gtctgatcgc agtgatgatg cttatctctt ctttacaacc cgtcacggta
     4681 ttgtgaagag aactagtgtt aaggagtttg ccaatattcg tcaaaatggt ctcaaagcgc
     4741 tgaatttaaa ggatgaagat gagttaatca atgtcttgtt ggcagaagga gatatggata
     4801 ttatcattgg taccaagttt ggttatgcag ttcgctttaa tcaatcagcc gttcgtggta
     4861 tgagccgtat cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac acagttgttg
     4921 gtgccagctt gattactgat caagatgagg ttcttattat cacagaaaaa ggatatggta
     4981 agcgtacagt cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga atgcagacag
     5041 ctaaaattac cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa ggggatgagg
     5101 atttgatgat tatcactgat acaggtgtca tgattcgaac caatcttgcc aatatttcac
     5161 aaacaggacg tgcaactatg ggagttaaag taatgcgcct ggatcaagat gctcagatag
     5221 tgactttcac aacggttgcg gtggcagaaa aagaagaagt tgggacagaa aacgaaacag
     5281 aaggtgaagc ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa aaaatctgct
     5341 gacaaatatc ctagcaggat ttctgatatt actgtcactg gctttgattt ttaatactca
     5401 aattcgaaat attttcatag tctggaatac caataagtat caagttagcc aggtatcaaa
     5461 agaaaaatta gaagaaaatc aggatacaga aggcaatttt gactttgatt ctgtcaaagc
     5521 tatctcttcg gaagctgttc taacttctca atggaatgct caaaaattac cagttattgg
     5581 gggaattgca attcctgaat tggaaatgaa tttgccgatt tttaaaggac ttgataatgt
     5641 taatctcttc tacggagctg gtacaatgaa acgcgagcaa gtaatgggag aaggaaatta
     5701 tagtctagct agtcaccata tctttggtgt tgataatgct aataaaatgt tattttctcc
     5761 tttagataat gctaaaaatg gcatgaagat ttatctaacc gataaaaata aagtttatac
     5821 ttatgaaata cgtgaagtca aacgtgtgac accggatcgt gttgatgaag ttgatgatag
     5881 agatggggtc aatgaaatca cattagtaac ctgtgaagac cttgctgcta cagaacgtat
     5941 tattgtcaaa ggtgatttga aagaaacaaa agattattca caaacatctg atgaaatcct
     6001 aacagctttc aatcaaccat ataaacaatt ttattaatac aaatcagtga aatcatggat
     6061 ttcactgatt ttttaagatt ttcataaaaa
//
LOCUS       DAVLYV010000095         6055 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLYV010000095 DAVLYV010000000
VERSION     DAVLYV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.095"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..379
                     /locus_tag="VYO70_001868"
     CDS             <1..379
                     /locus_tag="VYO70_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714035.1"
                     /translation="IVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKIDAYVV
                     NNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVWHFLCH
                     LDPMFVEIEDVEGYVKEEVGEIQ"
     gene            701..889
                     /locus_tag="VYO70_001869"
     CDS             701..889
                     /locus_tag="VYO70_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1714036.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            951..1709
                     /locus_tag="VYO70_001870"
     CDS             951..1709
                     /locus_tag="VYO70_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1714037.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNETHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            1767..2621
                     /locus_tag="VYO70_001871"
     CDS             1767..2621
                     /locus_tag="VYO70_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1714038.1"
                     /translation="MLGVIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVT
                     EAIMNGGQKDDFIDAMKKYGSMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2733..3617
                     /locus_tag="VYO70_001872"
     CDS             2733..3617
                     /locus_tag="VYO70_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1714039.1"
                     /translation="MKKVMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3780..3857
                     /locus_tag="VYO70_001873"
     CDS             3780..3857
                     /locus_tag="VYO70_001873"
                     /inference="COORDINATES: protein motif:HMM:NF014902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEV1714040.1"
                     /translation="MELSAIYHRPESEYAYLYKDKKLHI"
     gene            3921..5519
                     /locus_tag="VYO70_001874"
                     /pseudo
     CDS             3921..5519
                     /locus_tag="VYO70_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390924.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            5794..>6055
                     /locus_tag="VYO70_001875"
     CDS             5794..>6055
                     /locus_tag="VYO70_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714041.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYE"
BASE COUNT         2035 a          891 c         1280 g         1849 t
ORIGIN      
        1 tattgttcct ttatctaaat ttatgttcga tcctgaggaa aatgtaaaca ttatttggaa
       61 agagatttca aatttatccc tcaaaaatga tagagtgatc gaaggatatt caaaaattga
      121 tgcatatgtc gttaacaacc atgaagttaa agcctatgtc gaaacaagag aaacgaacta
      181 tcgtaaagca aaagacttct tagaaagccg cggatatgaa atcgacagaa gctttttcgg
      241 aagtgaagac ggtgaagcga ttctttacag aaaaaaaggg atcgaagttt ggcatttctt
      301 gtgtcactta gacccaatgt ttgtagagat tgaagatgta gaaggatatg ttaaagaaga
      361 agttggagag attcaatagt caaccaaagt tccttgtgag tatatacctt tgctagtaaa
      421 agagtcaaaa cgttataacg aaggggggga agaaaaccct tgagtgtaag tctttttctt
      481 gtttagaggg aaagtattag agtagccgct cataatttat actagatttg ataactgatt
      541 tgagacagat tttatttcta taaatattgt gaaaattttt taataccaaa gatttaaagt
      601 tactgtgtta aaaatcctct ccacttttaa actaatgtat tataaaagcc agttgacaca
      661 agtgtgacac ttgtgataga attaacttga ggtgatgaaa atggcaaccg ataaaaatag
      721 aataatgatt agcttggatg ataaaaatct agagaaactt gaaaatttag tagaagatgc
      781 tagagataga aggggaatgc gactaacaaa gtcccaggtt attgaattac tcctaaatac
      841 cgttgattat ttcgatgata ttatgggggc tatttactca aaaaaataag agcaaaattg
      901 ctattatttt ttacaaattg gtgtaacact agtgatatag gagtgatgaa atggttgttt
      961 atattagaca aagtaaatta ccaagtgagg tttcaattaa taaatacaat gctcaagtag
     1021 gtgcctactt acaaggagaa gaagttattt tatatcaatc tttttctgaa ataaaagagt
     1081 taacaagtga agatatagtt gtagattata taatggagac tagagcatta ctaaaaatga
     1141 tgggcttaaa cgttccggtt tatgactatc ctattgagct taaagagttt tatggtcgaa
     1201 agatttatgc tggtatttta ggagagattg tgaatatacc tgataattgg ggaaaattta
     1261 ttaagcctaa agctggttca aaagtcttta ctggaagagt tgttaatgaa acccatgatt
     1321 taataggtat tggtctacct ttcgactatc ctatatggat tagtgaggtt gtagaattca
     1381 tagctgaatg gcgttgtttt gtgttagatg gtcgcgtatt agatgttcga ccctatacag
     1441 gtgattatca tgcacaattt gatgcaagtg taattgatga agcgatatca tgttggaaag
     1501 atgcgccaat agcttatgga ctagatatcg gtgttactcg cgatggcaga acacttgttg
     1561 ttgaagtaaa tgatggttat gcattgggaa attatggcct atctccttta aaatcaatca
     1621 attttcatag agctaggtgg aaagaaatgg taaaacccta ttttgaaaaa aatgaaattt
     1681 ttaaaatcca acaggatgtt attttctaaa ataaatatgg tagataacca atataaaaag
     1741 catcaaaatg aacaaggagg aatctgatgc ttggagtaat tattggagat attgtcggtt
     1801 ctgtttacga atggaacaat atcaaaacaa aggactttcc tttatttcgg aaggactgct
     1861 tttttacaga tgacacggtt atgacctgtg ctgttacaga agcaattatg aatggtggac
     1921 aaaaagacga tttcattgac gcgatgaaga aatatggcag catgtatccg aatgctgatt
     1981 acggtgctcg gtttaatgca tggctaaaca gcgataatcg tgagccttat aatagctttg
     2041 ggaatggatc agctatgcgt atttctccat gtgcttggat catggactgt ggtttttatg
     2101 cgaaaactgg tatgtggcca tcatctagag gacttacgag tctttctgca gaggtaactc
     2161 ataatcatcc agaaggtgtc aaaggcgcta tggctacagc tgatgctatc tttctatgtc
     2221 gtttttactt tggtggttat tgtagagagt acgagcaatc aattaacgac aatcctacag
     2281 agtgtaaaag acgaattaag gattatatag aaaaagaata cgattacaat ctatctcaaa
     2341 ctttagatga aatccgtcct aactatcgtt ttaacgaaac atgtcaggaa actgtccctc
     2401 aagccattat cgcctttctt gaaagtagag acttcgaaga tgcgataaga aatgccatct
     2461 cacttggtgg cgacagtgat actctcgcag caatcacttg taacatagca gaggcagctt
     2521 acggtattcc tgattggatc aaggataagg cctattctta cttggatgaa cccttaaagg
     2581 atgtagttag gcgatgggaa aatagaataa aagcgtatta atctccagta cgtcagattt
     2641 ctcttttctg tattttcatt gtataatagg attacattta cttgcatacg acgaaatgta
     2701 aacatttctg tttaaaatta aggataaaat caatgaaaaa agtaatggta attatcaacc
     2761 ctacttctgg tggcgagaag gctttggatt acaaagaaaa gctggagaat aaagcaaaag
     2821 aatactttga atatgttgaa accaaaatta ccgaaaaagc gctggatgca acacattttg
     2881 ctgaagaagc ttctcgtgag cagtatgatg cagtggttgt gtttggtgga gatggaactg
     2941 tcaatgaagt catttcaggt attgatgaga gagactacat tcctaagtta gggattatcc
     3001 caggcggtac gggtaacctc attacaaaac ttttggaaat caatcaagac atcgatggcg
     3061 caattgacga actggatttt gatttaacca ataagattga tatcggtaaa gcaaatgaca
     3121 actattttgg ttatatcttt agtatcggtt ctctgcctga ggcgattcac aatgttgaaa
     3181 tagaggacaa aacaaaattc ggtattttaa cctatgctgt aaataccatg aagtctgtca
     3241 tgacagatca ggtctttaac attaaggttg agacagaaaa tggaaattat gttggtgaag
     3301 ctagccatgt tttggtcctt ttgacaaatt acttcgctga taagaaaatc tttgaagaaa
     3361 acaaggacgg ctatgccaac attttgattc tgaaagatgc ctctatattc tccaaattat
     3421 ccgtcattcc tgatttatta aaaggggatg ttgtcgcaaa tgataatatc gagtatatca
     3481 aagcgcgtaa tattaaaatc tcttcagata gtgaattgga gtcagatgtt gacggagata
     3541 aatcagataa cctacctgta gaaatcaaag tcctagctca gcgagtagaa gtattttcaa
     3601 aaccgaaaga ggattagtat atagagaaag ccttttttaa ggctttttgt atactttaaa
     3661 agatagttcc tttaacaacg gacattcctt gcaaatagtt tacaaaaata gtatactgga
     3721 tttattgagt ttgaaaacgt ttgcgtaaaa tttgaataaa tactttagga gacaaattga
     3781 tggaattgag tgctatttac cataggcctg agtcggagta tgcctatctt tataaggata
     3841 agaaactcca tatttgaatt cgaactaaga aaggggacat tgaaagcatc aacttgcact
     3901 atggggaccc ttttatcttt atggaggagt tttatcagga tacaaaagaa atggtcaaga
     3961 taacttctgg taccttattt gaccattggc aggttgaagt gtcagttgac tttgcacgta
     4021 tccagtatct ctttgagctc agagatacag aaggtcaaaa tattttgtat ggcgataaag
     4081 ggtgtgtgga aaattctcta gaaaatcttc atgcaattgg gaatggattt aagttgcctt
     4141 atcttcatga gattgatgcc tgcaaggttc ctgactgggt ttcaaatacg gtatggtatc
     4201 agatatttcc tgaaagattt gccaatggca atgctctatt aaacccagaa gggactttag
     4261 actgggattc atctgtcaca cctaagagcg atgatttctt tggtggtgat ttacagggga
     4321 ttattgatcg tatggattac ttgcaagact tgggtattac tggactatat ctttgtccca
     4381 tctttgaatc tacaagcaat cacaagtaca atacgacaga ttactttgaa attgaccgtt
     4441 attttggaga caaggagacc tttcgggaac tggtggatca agcgcatcat cgtggcatga
     4501 aagtcatgat ggatgcggta tttaatcata ttggttcgca atctcttcaa tggaaaaatg
     4561 tcgtcaaaaa tggcgaacag tctgcttata aggattggtt ccatattcaa caattcccag
     4621 tgacaactga aaagctagtt aataagagag acttacccta tcatgttttt ggtttcgagg
     4681 actatatgcc taagctaaat acagccaatc cagaggtcaa gaattatctt ttaaaggttg
     4741 cgacttattg gattgaagag tttaatatcg atgcttggcg tttggatgtg gctaatgaga
     4801 ttgaccatca gttctggaag gattttcgta aggcagtttt agctaaaaat cctgatcttt
     4861 atatcctagg agaagtctgg catacatctc agccttggct aaatggagat gagttccatg
     4921 ccgtcatgaa ttatccttta tctgatagta tcaaggacta tttcttacga ggaattaaga
     4981 agacagacca gttcatcgat gaaatcaatt gagagtctat gtattacaag cagcagattt
     5041 cagaggtcat gtttaatctc ttggattcac atgatacaga gcgaatcctg tggacggcca
     5101 atgaagatgt tcaactggtt aaatcagcct tagcctttct cttttaacaa aaaggaacac
     5161 cgtgcattta ttacggaacc gagctagcct tgactggagg accagatcca gattgtcgtc
     5221 gttgtatgcc ttgggaacgt gtatcaagtg acaatgatat gctgaacttt atgaagaggc
     5281 tgattaagat tcggaaatac gcgtcagtaa tcatttcgca tggcaagtat agccttcaag
     5341 aaatcaaatc tgatctagta gctctggaat ggaaatacga aggacggatc ctcaaagcaa
     5401 tattcaacca atcaacagaa gattatcttt tagagaaaga agcagtagca ctagcaagca
     5461 attgccaaga attggagaat cagcttgtca tctctccaga tggatttgtg attttctaaa
     5521 aactagttga tgaagattat ggtacatttc atatcttata tagtataata aggatagtta
     5581 ctaaacttgt aaaggagaac ttaaatgaat tgtagaggac atgaaacaag acaaagaatt
     5641 gttagagatt ttgaagttta gcctaaagca catattaagc tgttagcaaa tcaacaaaaa
     5701 catagtgatg caggagcaac tattgaagat gaatattatg tatttatcgc tgagagtaaa
     5761 attgatggca agaaggaagt tattcagtgt tgcatgggtg cggcaaggga ttttttagaa
     5821 ctaattaatc acaaagggct acctcttttt aatccgcttg taggtgattc tcatgtaaat
     5881 aatagacaag aatatgacaa tacagggagt ggaaatttac aacctgaaaa gtggaatgaa
     5941 actgcaaagc agctttataa tgttataatg tggttgatta ttttatggaa tgctaagccg
     6001 gatacacctt tatttaattt taaagacgaa gtaattaagt ataaaacata tgagc
//
LOCUS       DAVLYV010000096         5925 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLYV010000096 DAVLYV010000000
VERSION     DAVLYV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.096"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..258
                     /locus_tag="VYO70_001876"
     CDS             <1..258
                     /locus_tag="VYO70_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1714042.1"
                     /translation="DKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVNDQV
                     EIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            251..619
                     /locus_tag="VYO70_001877"
     CDS             251..619
                     /locus_tag="VYO70_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1714043.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            739..2007
                     /locus_tag="VYO70_001878"
     CDS             739..2007
                     /locus_tag="VYO70_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1714044.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            2004..3281
                     /gene="tilS"
                     /locus_tag="VYO70_001879"
     CDS             2004..3281
                     /gene="tilS"
                     /locus_tag="VYO70_001879"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1714045.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            3285..3827
                     /gene="hpt"
                     /locus_tag="VYO70_001880"
     CDS             3285..3827
                     /gene="hpt"
                     /locus_tag="VYO70_001880"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1714046.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            3843..5801
                     /gene="ftsH"
                     /locus_tag="VYO70_001881"
     CDS             3843..5801
                     /gene="ftsH"
                     /locus_tag="VYO70_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1714047.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMSGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         2060 a          884 c         1221 g         1760 t
ORIGIN      
        1 gataaatatt taaaagtatc gcgaattatc aagcgtcgta cagtcgcaaa ggaagtagca
       61 gataaaggta gaatcaaggt taatggaatc ttggccaaaa gttcaacgga cttgaaagtt
      121 aatgaccaag ttgaaattcg ctttggcaat aagttgctgc ttgtaaaagt actagagatg
      181 aaagatagta caaaaaaaga agatgcagca ggaatgtatg aaattatcag tgaaacacgg
      241 gtagaagaaa atgtctaaaa atattgtaca attgaataat tcttttattc aaaatgaata
      301 ccaacgtcgt cgctacctga tgaaagaacg acaaaaacgg aatcgtttta tgggaggggt
      361 attgattttg attatgctat tatttatctt gccaactttt aatttagcgc agagttatca
      421 gcaattactc caaagacgtc agcaattagc agacttgcaa actcagtatc aaactttgag
      481 tgatgaaaag gataaggaga cagcatttgc taccaagttg aaagatgaag attatgctgc
      541 taaatataca cgagcgaagt actattattc taagtcgagg gaaaaagttt atacgattcc
      601 tgacttgctt caaaggtgat aaaatggaaa atttattaga cgtaatagag caatttttga
      661 gtttgtcaga tgaaaagctg gaagaattgg ctgataaaaa tcaattattg cgtttacaag
      721 aagaaaagga aaggaagaat gcgtaaattc ttaattattt tgttgttacc aagttttttg
      781 accatttcaa aagtcgttag cacagaaaaa gaagtcgtct atacttcgaa agaaatttat
      841 tacctttcac aatctgactt tggtatttat tttagagaaa aattaagttc tcccatggtt
      901 tatggagagg ttcctgttta tgcgaatgaa gatttagtag tggaatctgg gaaattgact
      961 cccaaaacaa gttttcaaat aaccgagtgg cgcttaaata aacaaggaat tccagtattt
     1021 aagctatcaa atcatcaatt tatagctgcg gacaaacgat ttttatatga tcaatcagag
     1081 gtaactccaa caataaaaaa agtatggtta gaatctgact ttaaactgta caatagtcct
     1141 tatgatttaa aagaagtgaa atcatcctta tcagcttatt cgcaagtatc aatcgacaag
     1201 accatgtttg tagaaggaag agaatttcta catattgatc aggctggatg ggtagctaaa
     1261 gaatcaactt ctgaagaaga taatcggatg agtaaagttc aagaaatgtt atctgaaaaa
     1321 tatcagaaag attctttctc tatttatgtt aagcaactga ctactggaaa agaagctggt
     1381 atcaatcaag atgaaaagat gtatgcagcc agcgttttga aactctccta tctctattat
     1441 acgcaagaaa aaataaatga gggtctttat cagttagata cgactgtaaa atacgtatct
     1501 gcagtcaatg attttccagg ttcttataaa ccagagggaa gtggtagtct tcctaaaaaa
     1561 gaagataata aagaatattc tttaaaggat ttaattacga aagtatcaaa agaatctgat
     1621 aatgtagctc ataatctatt gggatattac atttcaaacc aatctgatgc cacattcaaa
     1681 tccaagatgt ctgccattat gggagatgat tgggatccaa aagaaaaatt gatttcttct
     1741 aagatggccg ggaagtttat ggaagctatt tataatcaaa atggatttgt gctagagtct
     1801 ttgactaaaa cagattttga tagtcagcga attgccaaag gtgtttctgt taaagtagct
     1861 cataaaattg gagatgcgga tgaatttaag catgatacgg gtgttgtcta tgcagattct
     1921 ccatttattc tttctatttt cactaagaat tctgattatg atacgatttc taagatagcc
     1981 aaggatgttt atgaggttct aaaatgaggg aaccagattt tttaaatcat tttctcaaga
     2041 agggatattt caaaaagcat gctaaggcgg ttctagctct ttctggtgga ttagattcta
     2101 tgtttctatt taaggtattg tctacttatc aaaaagagtt agagattgaa ttgattctgg
     2161 ctcatgtgaa tcataagcag agaattgaat cagattggga agaaaaggaa ttaaggaagt
     2221 tggctgctga agcagagctt cctatttata tcagcaattt ttcaggagaa ttttcagaag
     2281 cgcgtgcacg aaattttcgt tatgattttt ttcaagaggt tatgaaaaag acaggtgcga
     2341 cagctttagt cactgcccac catgctgatg atcaggtgga aacgattttg atgcgcttga
     2401 ttcgaggaac tcgcttgcgc tatctatcag gaattaagga gaagcaagta gtcggagaga
     2461 tagaaatcat tcgtcccttc ttgcattttc agaaaaaaga ctttccatca atttttcact
     2521 ttgaagatac atcaaatcag gagaatcatt attttcgaaa tcgtattcga aactcttact
     2581 taccagaatt ggaaaaagaa aatcctcgat ttagggatgc aatcttaggc attggcaatg
     2641 aaattttaga ttatgatttg gcaatagctg aattatctaa caatattaat gtggaagatt
     2701 tacagcagtt attttcttac tctgagtcta cacaaagagt tttacttcaa acttatctga
     2761 atcgttttcc agatttgaat cttacaaaag ctcagtttgc tgaagttcag cagattttaa
     2821 aatctaaaag ccagtatcgt catccgatta aaaatggcta tgaattgata aaagagtacc
     2881 aacagtttca gatttgtaaa atcagtccgc aggctgatga aaaggaagat gaacttgtgt
     2941 tacactatca aaatcaggta gcttatcaag gatatttatt ttcctttgga cttccattag
     3001 aaggtgaatc aattcaacaa atacctgttt cacgtgaaac atccatacac attcgtcatc
     3061 gaaaaacagg agatgttttg attcaaaatg ggcatagaaa aaaactcaga cgtttattta
     3121 ttgatttgaa aatccctatg gaaaagagaa actctgctct tattattgag caatttggtg
     3181 aaattgtctc aattttggga attgcgacca ataatttgag taaaaaaacg aaaaatgata
     3241 taatgaacac tgtactttat atagaaaaaa tagataggta aaaaatgtta gaaaacgata
     3301 ttaaaaaagt cctcgtttca cacgatgaaa ttacagaagc agctaaaaaa ctaggtgctc
     3361 aattaactaa agactatgca ggaaaaaatc caatcttagt tgggatttta aaaggatcta
     3421 ttccttttat ggctgaattg gtcaaacata ttgatacaca tattgaaatg gacttcatga
     3481 tggtttctag ctaccatggt ggaacagcaa gtagtggtgt tatcaatatt aaacaagatg
     3541 tgactcaaga tatcaaagga agacatgttc tatttgtaga agatatcatt gatacaggtc
     3601 aaactttgaa gaatttgcga gatatgttta aagaaagaga agcagcttct gttaaaattg
     3661 caaccttgtt ggataaacca gaaggacgtg ttgtagaaat tgaggcagac tatacctgct
     3721 ttactatccc aaatgagttt gtagtaggtt atggtttaga ctacaaagaa aattatcgta
     3781 atcttcctta tattggagta ttgaaagagg aagtgtattc aaattagaaa gaataatctt
     3841 taatgaaaaa acaaaataat ggtttaatta aaaatccttt tctatggtta ttatttatct
     3901 ttttccttgt gacaggattc cagtatttct attctgggaa taactcagga ggaagtcagc
     3961 aaatcaacta tactgagttg gtacaagaaa ttaccgatgg taatgtaaaa gaattaactt
     4021 accaaccaaa tggtagtgtt atcgaagttt ctggtgtcta taaaaatcct aaaacaagta
     4081 aagaaggaac aggcattcag tttttcacgc catctgttac taaggtagag aaatttacca
     4141 gcactattct tcctgcagat actaccgtat cagaattgca aaaacttgct actgaccata
     4201 aagcagaagt aactgttaag catgaaagtt caagtggtat atggattaat ctactcgtat
     4261 ccattgtgcc atttggaatt ctattcttct tcctattctc tatgatggga aatatgggag
     4321 gaggcaatgg ccgtaatcca atgagttttg gacgtagtaa ggctaaagca gcaaataaag
     4381 aagatattaa agtaagattt tcagatgttg ctggagctga ggaagaaaaa caagaactag
     4441 ttgaagttgt tgagttctta aaagatccaa aacgattcac aaaacttgga gcccgtattc
     4501 cagcaggtgt tcttttggag ggacctccgg ggacaggtaa gactttgctt gctaaggcag
     4561 tcgctggaga agcaggtgtt ccattcttta gtatctcagg ttctgacttt gtagaaatgt
     4621 ttgtcggagt tggagctagt cgtgttcgct ctctttttga ggatgccaaa aaagcagcac
     4681 cagctatcat ctttatcgat gaaattgatg ctgttggacg tcaacgtgga gtcggtctcg
     4741 gcggaggtaa tgacgaacgt gaacaaacct tgaaccaact tttgattgag atggatggtt
     4801 ttgagggaaa tgaagggatt atcgtcatcg ctgcgacaaa ccgttcagat gtacttgatc
     4861 ctgccctttt gcgtccagga cgttttgata gaaaagtatt ggttggccgt cctgatgtta
     4921 aaggtcgtga agcaatcttg aaagttcacg ctaagaacaa gcctttagca gaagatgttg
     4981 atttgaaatt agtggctcaa caaactccag gctttgttgg tgctgattta gagaatgtct
     5041 tgaatgaagc agctttagtt gctgctcgtc gcaataaatc gataattgat gcttcagata
     5101 ttgatgaagc agaagataga gttattgctg gaccttctaa gaaagataag acagtttcac
     5161 aaaaagaacg agaattggtt gcttaccatg aggcaggaca taccattgtt ggtctagtct
     5221 tgtcgaatgc tcgcgttgtc cataaggtta caattgtacc acgcggccgt gcaggcggat
     5281 acatgattgc acttcctaaa gaggatcaaa tgcttctatc taaagaagat atgaaagagc
     5341 aattagctgg cttaatgggt ggacgtgtag ctgaagaaat tatctttaat gtccaaacta
     5401 caggagcttc aaacgacttt gaacaagcga cacaaatggc gcgtgcaatg gttacagagt
     5461 acggtatgag tgaaaaactt ggcccagtac aatatgaagg aaaccatgct atgtctggtg
     5521 cacagagtcc tcaaaaatca atttcagaac aaacagctta tgaaattgat gaagaggttc
     5581 gttcattatt aaatgaggca cgaaataaag ctgctgaaat tattcagtca aatcgtgaaa
     5641 ctcacaagtt aattgcagaa gcattattga aatacgaaac attggatagt acacaaatta
     5701 aagctcttta cgaaacagga aagatgcctg aagcagtaga agaggaatct catgcactat
     5761 cctatgatga agtaaagtca aaaatgaatg acgaaaaata accctgagag aggctggagc
     5821 ctctcttttt tgtgcagttt aggagctaaa gggaacagaa tggagaaaat ggaacaaatg
     5881 tgttttctaa tctgttagac tgtatctaga aaggggaaaa ttatg
//
LOCUS       DAVLYV010000097         5664 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLYV010000097 DAVLYV010000000
VERSION     DAVLYV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5664
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.097"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..224
                     /locus_tag="VYO70_001882"
     CDS             <1..224
                     /locus_tag="VYO70_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1714048.1"
                     /translation="FQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFG
                     FRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            complement(323..1945)
                     /gene="groL"
                     /locus_tag="VYO70_001883"
     CDS             complement(323..1945)
                     /gene="groL"
                     /locus_tag="VYO70_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1714049.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(1961..2245)
                     /gene="groES"
                     /locus_tag="VYO70_001884"
     CDS             complement(1961..2245)
                     /gene="groES"
                     /locus_tag="VYO70_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1714050.1"
                     /translation="MLKPLGDRLVLKVEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2401..2778)
                     /locus_tag="VYO70_001885"
     CDS             complement(2401..2778)
                     /locus_tag="VYO70_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1714051.1"
                     /translation="MIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGEREVDFVN
                     MVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLLESRAQR
                     AMRENNAGQDLADLVLEEEELPF"
     gene            complement(2805..3047)
                     /locus_tag="VYO70_001886"
     CDS             complement(2805..3047)
                     /locus_tag="VYO70_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714052.1"
                     /translation="MKILNIKLANVEQTDSGFEHWVDVTYNVPILKNEYTVRLLLLFG
                     FEIEDPEVIEYLVNAWKYRELVLHSVRMYDMEREAS"
     gene            complement(3279..4235)
                     /gene="lytA"
                     /locus_tag="VYO70_001887"
     CDS             complement(3279..4235)
                     /gene="lytA"
                     /locus_tag="VYO70_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1714053.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTVQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLASWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDGSGYMLAD
                     RWKKYTDGNWYWFDNSGEMATGWKKIAEKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPDGLITVK"
     gene            complement(4235..4570)
                     /locus_tag="VYO70_001888"
     CDS             complement(4235..4570)
                     /locus_tag="VYO70_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEV1714054.1"
                     /translation="MQQITEIITNGAISILVILAGIAVKAVKEYLVKKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            complement(4574..4873)
                     /locus_tag="VYO70_001889"
     CDS             complement(4574..4873)
                     /locus_tag="VYO70_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714055.1"
                     /translation="MPIEEAEKIAQSQVAWAILFILLFFIIIRYLIKTSDKREKKIMD
                     LHEQSKADSNRREERLMTHLEKTTTELTTITHTVGDIQKEMVRMNDRMEEIEKGE"
     gene            complement(4882..5232)
                     /locus_tag="VYO70_001890"
     CDS             complement(4882..5232)
                     /locus_tag="VYO70_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714056.1"
                     /translation="MAETNQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTVPTVEPKE"
     gene            complement(5235..5438)
                     /locus_tag="VYO70_001891"
     CDS             complement(5235..5438)
                     /locus_tag="VYO70_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714057.1"
                     /translation="MNTEQLNQALRMTISEISTTSTNSMIESNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
BASE COUNT         1463 a         1317 c         1046 g         1838 t
ORIGIN      
        1 tttttcagac tgtctttaaa accttcctca aagataaaga aaagattgtc aacgcccttc
       61 aactacacta ttctaatgcc aaactggaag cgaccaataa tctcatcaaa cttatcaagc
      121 gcaatgcctt tggttttcgg aactttgaaa acttcaaaaa acggattttt atcgccctga
      181 atatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagctgact tcaacccact
      241 acagttgaca aagagcctat aaaaagaagg gttagtcatt ccctcctttt gtgtttttta
      301 taagttgttt tctatagaaa gcttacatca tcccgcccat catacttgga tccattgctg
      361 gagctggggc tactggttct ggtttattgg ctacgactgc ttctgttgtc aaaatcaagc
      421 tggctacaga tgctgcattt tgtagggctg aacgactcac tttaactgga tcaatgatac
      481 cttgatcaat catgttaacc cactcgccag ttgctgcgtt aaatcctata ccaagctcag
      541 catttttcaa acgatcgata acgatagatc cttcaaatcc tgcattgtga gcaatttgac
      601 gaacgggttc ttccaaagca cggagaacaa tattacgtcc tgttgcttca tctcctgtca
      661 attccaaggt agcaacagct ggaatcacat tggcaagagc tgttccaccg cctgcaacaa
      721 taccttcttc aacagctgca cgagtagcgt tgagggcatc ttcaatgcgg agtttcattt
      781 ctttcaactc agtttcagtt gcggctccga ccttaataac cgctacacca cctgacaatt
      841 tggccaagcg ttcttgcaat ttttcacggt caaattcaga agttgtagtt tcgatttgag
      901 acttgataac cgcaacacgg tgagaaatcg cttcaggatt tcctgcacct tctacaataa
      961 ccgtgctatc tttgtccacg gtcactctcg ctgcttgacc aagagcttca attgtcgcat
     1021 ctttcaactc aagaccaagg tcttctgtga taactgttcc gcctgttaag atggcgatat
     1081 cttcaagcat ggctttgcga cggtcaccaa aaccaggtgc cttgactgct actacgttga
     1141 aggttccacg aatcttgttc aaaacaagag ttggaagagc ctcgccatcc acatcatccg
     1201 caataatcaa gagtggacga ttgctttgga gaatgctttc caaaagtggc aagatctctt
     1261 ggatattgga aattttcttg tctgtaatca aaatgtacgg attttcaagg tcagccacca
     1321 ttttttcgct atctgtcacc atgtactgtg aaaggtaacc acggtcaaac tgcattcctt
     1381 ctacgacttc aagctctgtt tccataccac gtgactcttc gatggtgatg acaccgtctt
     1441 tgccaacttt ttccattgct tcagagatgt actcaccaac tttttcagaa cgagaagata
     1501 cggctgcaac ttgagcgata gcttctttat tggcaacagg gatggcgttg tttttcaaag
     1561 cttcaactgc tgcggcaact gctgtttcaa tcccacgacg aataccgatt ggatttgcac
     1621 ctgctgtgac gtttttgatt ccttcacgga cgatagcttg ggtcaagact gttgcagtcg
     1681 tagtcccgtc acctgcgata tcattggttt tagaagctac ttctgatacc aatttggcac
     1741 ccatattttc aaaatggtct tctaattcaa tttctttggc aatagtcaca ccgtcattgg
     1801 taatcaatgg tgagccaaat gatttttcaa gaacgacatt gcgacctttt ggtcccaagg
     1861 ttactttaac agtatctgca aggatatcga caccacggac catagctgaa cgggcatctg
     1921 atgaaaattt aatttctttt gacatactta ctttctcctt ctattcttca atgattgcca
     1981 agatgttagc ttcgcctacg atgatatact tttcatcgcc atctttgaca tcaagacctg
     2041 cgtgggcttc aactaagaca cggtctccag ttttaacact tggagcaacc aagtcaccgt
     2101 tcaaggtacg aacaccttgt ccagtagcca caacttgggc tgttttggtt ttttcttggg
     2161 ctgaacctgc gagaacaaag cctccaacag tttgttcttt ttcttctact tttaagacca
     2221 aacggtcccc taatggtttc aacatctgat ttcctccata atgagataga tattattagc
     2281 actctttata cctgagtgct aattcatagt tctattgtat cacttagtca gaaataatca
     2341 agaaaaaagt ctgactttct caagataaaa agcctgagac caactcagac tttttaattc
     2401 ttaaaatggc aattcttcct cttccaagac caaatctgcc aaatcttggc ctgcattatt
     2461 ttcacgcata gcacgttggg cacgactttc caagagttgg aatcctgtca caaggacttc
     2521 ggttacataa ttcatctgac catttttctc aaagcgacgg gtacgcaatt ctccatcaac
     2581 ggaaatgaga ctacctttgg ttgcgtagct tgccaaagtt tctgctagtc tgccccatag
     2641 gaccatattg acaaaatcaa cttcacgttc accgttttgg tctttgtaac gacggttgac
     2701 agcgatagtt gctcgcgcta ctgacttgtc attgttggtt ttgtgcaatt ctggtgtaga
     2761 cgttaaacgt ccaatcaaga taagttagag ttagtaacag tattttaact tgcttctctc
     2821 tccatatcat acatcctcac agaatgtaag accagctcac gatatttcca agcgttgacc
     2881 aggtattcaa tcacttcagg atcttcaatt tcaaaaccaa agagcagcaa caatctaacc
     2941 gtgtattcgt ttttcaaaat tgggacgttg taagtcacat ccacccaatg ctcaaagcct
     3001 gaatctgtct gctctacatt tgctagttta atatttaaaa tcttcatttt tattcctcct
     3061 acttatctat tcgtaaaaga agataaaaag ttatgaaaaa atcattactt ttttaattca
     3121 gacagtactt tcagagcaat tttgttaatg tcaacaaaat tggcaaccaa gcgatttaat
     3181 actatttgtt gacgccaaca agtcaatttt cagacaaaca aaaaaaccgc aagcctgagc
     3241 ctgcggtgaa agaacaattt agaaagtttc ctttctattt atttaactgt aatcaagcca
     3301 tctggctcta ctgtgaagtc tggcttatct gccagtgttc cgtctggttt gaggtagtac
     3361 cagcctgttc cgtctgcgga ctggataaag gcatttgata ccatagcgcc ttctttagcg
     3421 tctaagtagt accaagtgtc cttgtacttg acccagcctg tcttcatggc accttcttcg
     3481 ttgaaatagt accacttctc agcaattttc ttccagcctg tagccatttc gcctgagttg
     3541 tcaaaccagt accagttgcc gtctgtgtac ttcttccagc ggtctgcaag catatagcct
     3601 gagccatcga aataatacca ggtaccgttg attttctcaa acttatcttt tggataagag
     3661 ccgtctgaat gtacgtacca gtagccagtg tcattcttct gccagcctgt ttcaatcgtc
     3721 aagccgttct caatatcatg cttaaactgc tcacggctaa tgccccaact tgcaagatat
     3781 ggatatggat ccacatggtc tgagtggttg tttggttggt tattcgtgca atactcgtgc
     3841 gttttaattc cagctaaact ccctgtatca agcgttttcg gcaaacctgc ttcatctgct
     3901 agattgcgta agagttcgat ataaaggcgg tagtccgtca tgaactcttc tttagttgaa
     3961 tggctttcaa tcagttcaac cgctgcgtaa ctctcagtat tccaaccgcc cccaacatcc
     4021 caacttccgt tgttcacagg acctacttgc atgacacggc cgtttccgac aacatgtgaa
     4081 aagaacccta gttcagggtc ctttctatag tgataatccg cttcattctg tacggttgag
     4141 ttgcggttac ctgttgagtg agcatgtact tgtcgataag gctgcacccc aacctggggc
     4201 aagcctgttc gtagtctgtt tctatcgata tccattactc ttgtcctttc caagcgtcat
     4261 tcatctgctt cacggctgac tccacaaagg tgtctaagtc cttgtccgtc atactgatat
     4321 tgtattttgt aagctcagca cggactttag cacgagcctg tgccagtttt tcatctccct
     4381 tgtagccagt ttcagcagct acctgctcca cggcattaac tgcgttctta gccaagattt
     4441 caacaatctt gatagtcttt tctccacctt ttttgacgag gtattccttg actgccttga
     4501 ctgcgatacc agccaaaata acaaggatgc tgattgctcc attagtaatg atttcagtaa
     4561 tttgttgcat ttgttattct cctttttcga tttcttccat gcggtcgttc atgcgaacca
     4621 tttctttttg aatgtctccg accgtgtgag tgattgtggt taattctgta gtggtctttt
     4681 ctaggtgagt catcaaacgc tcttctcgtc tattagagtc ggcctttgat tgctcgtgca
     4741 aatccataat cttcttctct cgcttgtccg aagtcttgat aagatatcga atgataataa
     4801 aaaaaagcaa gataaacaaa atcgcccaag ctacctgact ttgagcgatt ttttcagctt
     4861 cttcaattgg catacaacct cctattcttt aggttctacc gttggaaccg tccagtcagg
     4921 attgccctct gcatcaaatt tcatgatata gaattcatga ttcaacagaa cggctacgtt
     4981 gattgttgcg attgtaccac cccactggtt gaatgcccaa acggtttcaa catccttgaa
     5041 ttggcgacgg ccatttacga tcacaggacg tttttgaacg tcacgataca tatagaagtc
     5101 atcgcttaca ttcttgcaac gaatgaactc tccattttct ttcatgtagc gcaaagcact
     5161 cgcaagatca aatggttctg tgatttttgt aaggtctagt aagttatctg tgttttgatt
     5221 tgtttctgcc atgtctattc tcctttgtct gctggtttag tttgttcatc aagcagagct
     5281 tccagctcat ccactcgtgc ttgaagtctt tgattctctt ccctttgctc atccaactga
     5341 atactcaaga tattactttc aatcatcgaa tttgttgaag ttgttgatat ttcactaatt
     5401 gtcattcgta aggcttggtt aagctgttct gtgttcattt tctaagttct ccaatctgtg
     5461 tgttcgtttt ctattttcaa gagcaagctc ctgaattgct ttaagtgcga tattagtcaa
     5521 catgttgtta ttcatgctat tgttttctcc attttttcta ttttttgatt taattcttga
     5581 atagccttga ttaagtaagg aactaaagcg gtatagtcga tatgcagata gccatctgga
     5641 ttctcaggat ctcgtgagac aatt
//
LOCUS       DAVLYV010000098         5589 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLYV010000098 DAVLYV010000000
VERSION     DAVLYV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.098"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            270..449
                     /locus_tag="VYO70_001892"
                     /pseudo
     CDS             270..449
                     /locus_tag="VYO70_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(664..1719)
                     /locus_tag="VYO70_001893"
     CDS             complement(664..1719)
                     /locus_tag="VYO70_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEV1714058.1"
                     /translation="MTLLLNKIYRQDVESSLETIVNINDLNGKSILITGASGLIGSSV
                     IDQLLLLNEIRNFNIKIFCCGREFESLEKRFGRQTEYLHFVFYDATKAPHFDFAVDYI
                     IHAASPASPELYVNQPVETMASNFLGMYNLLEYAKNAKVSNLVYISSSEVYGISTSNN
                     PLDENYIGTVDHLSVRSSYASSKRATETLCVSFASEYGIKIMIVRPGHIYGPSAKDSD
                     NRVSSFFMTEAIAGRDIVIKSTGSQLRSYCYSLDCASAILSVLLSGGSGQAYNVSNPN
                     SIITIKQMATCIADASGVRVLYDIPITQERKRENPMVNASLDSEKIESLGWSGLFSAE
                     VGFHHTFSILKELSSEK"
     gene            complement(1722..2441)
                     /locus_tag="VYO70_001894"
     CDS             complement(1722..2441)
                     /locus_tag="VYO70_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1714059.1"
                     /translation="MKVAILTASGIGSRIGQDIPKQFIHVENKPVIIYTLEKFQNHPE
                     IDEICIVILKGWDQMVKAYAEQFGITKLKMITFGGKSGQESIYNGLREVKKAHPNDDV
                     TVLIHDGNRPLVSNDIISNALATYQQFGNAVAAIPTTEVVFVLENPQSTSSTEALNRD
                     LLRRTQTPHVYHLDNILSLHEKALENGITDVAASCQLMQLFGEKSYFSLGTEKNLKIT
                     TVEDLDIFKALLSSTRDKWIK"
     gene            complement(2442..3338)
                     /locus_tag="VYO70_001895"
     CDS             complement(2442..3338)
                     /locus_tag="VYO70_001895"
                     /inference="COORDINATES: protein motif:HMM:NF016852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HEV1714060.1"
                     /translation="MAVKLKYDKSDITFLDDDELKKYQSELLKMISDVFTFFDENRIA
                     YSLSGGSILGAIRHKGFIPWDDDVDINIPRESYDKLFSLFELDNSLSRKYYLQSAKSH
                     PELGLHVSQIRKKGTVARRKYDHSAEECGISIDLYIVENVYNNPVKRFFQGYTSMFLT
                     FALASVRETKNHALMKEMFRLEGRKLNYSAGKLMVGWFFGIIPIEKWLNWLDKCNSSC
                     KDSHTKYVSIPTGRKHFRRETYLRENMNIYKKVPFETLMVNVPVWSEEYLEMFYGKDY
                     MVVPPVDKREQHLFLELSYGEE"
     gene            complement(3344..4516)
                     /locus_tag="VYO70_001896"
     CDS             complement(3344..4516)
                     /locus_tag="VYO70_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEV1714061.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKAIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIVYDIAAINRAIKIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQAIGGTLLVNPDGHEWLRAKWSAPVRRYWKISEGLMVKHA
                     DLLVCDSKNIEKYIQEDYKQYQPKTTYIAYGTDTTRSILKSSDEKVRSWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASNSKKDFVLITNVEQNKFYNQLLAKTGFDKDPRV
                     KFVGTVYEQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDELSQVIEKVEQFDAKMIDELDRQSNQRIADSFTWEKIVTDYEKVFKG"
     gene            complement(4547..>5589)
                     /locus_tag="VYO70_001897"
     CDS             complement(4547..>5589)
                     /locus_tag="VYO70_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1714062.1"
                     /translation="LYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLES
                     DELSWKLVAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDVGEII
                     SRFETMGIDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICG
                     ATIGLILFAIASLVLVPLIRKDGGPAIFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQ
                     LMDQNTMQGGMFKIDNDPRVTKIGRFIRKTSLDELPQFWNVFIGDMSLVGTRPPTVDE
                     YDQYTPEQKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTIWKDIEILLK
                     TVKVVFMRDGAK"
BASE COUNT         1785 a         1141 c          783 g         1880 t
ORIGIN      
        1 tgttctgttt gcgggttaca tccatttacc atatgtacat accctaatca atccattcca
       61 cggagttcaa tatatccaaa taaatacagt tttcccttct ctataaaatt ccatcagtgg
      121 aatctctatc tttctatgta ctctcataat attcataaca aaagatactc tcgcatgttt
      181 ttaacatata gtcctaacat ccccattgat aatatagtgt cgttttttca atagatttcc
      241 tgcgaaataa aatatggtat aatagttcta tgaattatga agcaagtaaa caactaactg
      301 atgtacgatt taagcgtctt gttgacgttc agcgtaccat ttttgaagag atgttagctg
      361 tattaaaaac agcttatcaa cttaaacacg caaaaggtgg acaaaaacct aaattaagcc
      421 tagaagacct tcttaaggcc actcttcaat aaaaaaactt ctccaaaatc ctcttggata
      481 gcttatatca aaaattgact atctttttct tcgtattctt taataattgc tcttatttga
      541 tcactagaat tgtcatcacg aatctatagt ttccatgctg taatggtttg aaaaaggata
      601 ctctccaact gttcacgtat aaattcttct ccattgtatg ttgtcatcat gattaaaata
      661 ctatcatttt tcacttgata actcctttaa aatactaaat gtgtgatgga agcctacttc
      721 agctgaaaat agacctgacc atcccaagga ctcaattttc tcactatcta agcttgcatt
      781 taccatagga ttttcccgct ttcgctcttg agttataggt atatcataaa gaactctgac
      841 tcctgaagca tctgcaatgc aagttgccat ctgttttata gtaataattg aatttggatt
      901 ggaaacatta taagcttgtc ctgatccacc cgaaagaaga acacttagaa ttgcactggc
      961 acaatccaaa ctataacaat aggatcttaa ctgactacct gtacttttta tgacaatatc
     1021 acgcccagca atcgcttctg tcataaaaaa tgatgataca cgattatcag agtccttggc
     1081 actaggacca tagatatgtc ctggccttac tatcattatt ttgattccat actcggaagc
     1141 aaaactaaca caaagtgttt cagtagctct ttttgaactg gcataagaag accgtacgct
     1201 aagatgatca acagttccaa tataattttc gtccaaagga ttattactag tgctaattcc
     1261 ataaacttca ctagatgaga tataaactag atttgaaact ttagcatttt tggcgtattc
     1321 cagtagatta tacataccca gaaaattact agccattgtt tctacaggct gattaacgta
     1381 taattcagga ctagcaggac tagcagcatg aataatgtag tccactgcaa aatcaaagtg
     1441 aggtgcttta gtcgcatcat agaacacaaa gtgtaaatac tctgtttgtc taccaaatcg
     1501 tttttctaat gattcaaact ctcttccaca acaaaaaatt ttaatgttaa aattccttat
     1561 ttcatttagc aataataact ggtcaatgac acttgaacca atcaaaccag aagctccagt
     1621 aattaaaatt gacttgccat tcaagtcatt aatattgacg attgtttcta gagaagattc
     1681 tacatcttgt ctatatattt tatttaataa taatgtcatt actatttaat ccacttatct
     1741 cgagttgaac tcaataaggc tttaaaaata tcgagatctt ctacagtcgt aattttcaag
     1801 tttttctctg ttccaagaga gaaataactc ttttcaccaa atagttgcat gagttggcat
     1861 gatgctgcaa catcagttat cccattttct aatgcttttt catgaagcga tagaatatta
     1921 tcaagatggt aaacatgagg agtttgtgtt cgtctcaaca agtcacgatt caatgcttct
     1981 gtggacgaag ttgattgtgg gttttctaaa acaaatacta cctcagtcgt tggtatcgct
     2041 gcaacagcat ttccaaactg ttggtatgtt gctaaagcgt tgctaataat atcattgctc
     2101 actaaaggac gattcccgtc atgaattaat acagtcacat catcattggg atgagctttc
     2161 ttaacttctc taagaccgtt ataaattgat tcttgaccag acttcccccc aaaagttatc
     2221 atctttaact ttgtaattcc aaattgttcc gcataagctt taaccatctg atcccagcct
     2281 tttaaaataa caatacagat ttcatcaatt tctggatgat tttgaaattt ttcaagggta
     2341 tagataatga ctggcttatt ttcaacatga ataaattgtt tagggatatc ttgaccaatt
     2401 cggcttccaa tgcctgaagc tgttaaaatc gctactttca tctattcttc tccgtaactc
     2461 aattctaaaa acaaatgttg ctctctctta tcaacaggag gaacgaccat ataatcttta
     2521 ccgtaaaaca tttctaaata ctcttctgac caaacaggaa cattcaccat caaagtttca
     2581 aaaggaacct ttttataaat attcatattc tctcgcaagt aagtctcgcg tctaaagtgt
     2641 ttccttcctg taggtatgga tacatatttt gtatgtgaat ccttacagct tgaattacat
     2701 ttatcaagcc aattcaacca cttttcaatt ggaataattc caaaaaacca accaaccatt
     2761 aactttccag ctgaataatt tagttttcta ccttccagtc taaacatttc cttcatcagg
     2821 gcatgattct ttgtttctcg tacagaagcc aaggcaaaag tcaaaaacat acttgtataa
     2881 ccttgaaaga atcgtttcac tggattattg tatacatttt ctacaatgta aagatctata
     2941 gaaataccgc actcctcagc cgaatgatcg tatttcctac gagcaactgt tccttttttt
     3001 ctaatttgag aaacatgaag tcccaattct ggatgacttt ttgctgattg aagataatat
     3061 ttacgactaa gactattgtc taactcaaat agagaaaata gtttatcgta actttccctc
     3121 ggaatattga tatcaacatc atcatcccaa gggataaacc ctttatgacg gattgcccct
     3181 aaaatgctac ctccacttaa agaatacgct atcctatttt catcaaaaaa agtaaacaca
     3241 tctgatatca ttttcaacaa ttctgattgg tattttttca attcgtcatc atctaaaaat
     3301 gtaatatctg atttatcata ttttaatttt actgccatta actctaacct ttaaatactt
     3361 tctcgtagtc tgtgacaatc ttttcccaag tgaaagaatc cgcaattctc tgatttgatt
     3421 gtctatctaa ctcatcaatc atttttgcat caaattgttc aactttctcg attacttggg
     3481 aaagctcatc ttttttccaa taaatcgcac tttgctcccc aacttctcgg ttaaagccaa
     3541 catctagcaa taaattcagt ttagttgatg ccagagcttc taaaagtgaa ggattggttc
     3601 caccaacttc atgcccgtgg aagtaagcaa aggcgttttc acgaatatat ttaagaagct
     3661 cttgctcata gactgtacct acaaatttta ctcgtgggtc cttatcaaaa ccagtttttg
     3721 ccaacaactg attataaaat ttattctgtt ctacatttgt gattaagaca aagtccttct
     3781 tagagttaga tgccaaaaag ccacgaatca tagattcata gttgttttct ggtacaaaac
     3841 gtcctacaac tagataatac tcgttctcag aaacattctt ctctttgaac caagaacgta
     3901 ctttttcgtc actactcttt aatatggagc gtgttgtatc agtcccataa gcgatatagg
     3961 ttgtcttagg ttgatactgt ttataatctt cttggatata tttttcaata ttcttactat
     4021 cacacactaa aagatctgca tgtttgacca taagaccttc ggaaattttc caataacgac
     4081 gaactggagc gctccactta gctcgtaacc actcgtgacc atctggatta actagaagag
     4141 tcccacctat cgcctggatt tttttcttaa ttccatggat gaacggaccg attcgacaag
     4201 ctagaatata gaagattgga tcttcatcct tattttcttt ggcaatttta atagctctgt
     4261 taattgccgc gatatcatat actatggctc gcgctggacc aatattagga acatcgacgt
     4321 tataacagat agcaccatta tgttcaaaaa catcctcagt agtccctgat tttgcagagt
     4381 tttcacgcat acaagccaca taatattgga tagccttatc ttgttggaag gctgttaatt
     4441 tttcaacaaa agtttcaaat cctccatact tagcaggaat tccttttgaa ccaatgatat
     4501 aaactgactt tttcatttct ctcctaatat gatggatata cagaaattac ttcgctccat
     4561 ctctcataaa tacaacttta acagttttaa gcaaaatttc aatatctttc cagattgtcc
     4621 aattatcaat ataagccaca tctaatttta caacatcatc aaaatcggtt attttactac
     4681 ggccgctaac ctgccataaa cctgtaatac caggtttaaa gctgagtcga cgtttctgtt
     4741 ctggagtata ctgatcatac tcgtctactg taggtggacg tgttcccacc aaactcatat
     4801 ctcctataaa gacattccaa aactgcggca attcatccaa actggtttta cgaataaagc
     4861 gaccaatttt tgtaacacga ggatcattgt ctatcttaaa cataccacct tgcatcgtat
     4921 tttgatccat caactgttct ttgatagctt cagcatcgat ccgcatcgaa cggaatttat
     4981 aaaaggtaaa atgtcgacca tttttcccta tacgagtttg agcaaaaata gctggtccgc
     5041 catctttacg aatcaatgga actaaaacta gactagctat agcaaaaaga ataaggccaa
     5101 tagtggcacc acaaatatcg agaattctct ttgaaatcac atgactagtt ttataaaaat
     5161 ttgtagagaa agtgactaca ttcaatccca ccatctcatg aatttgttta ttgcgaccca
     5221 aattcttatc aaatgcttta agatttacag ttacatctat ccccattgtc tcaaacctag
     5281 agataatttc tccaacatcg tagctctctc ctggaagatt gacaaacacc tcatccacaa
     5341 cttcatgcgt tgcaaattca ataatttttt ccttttcaat tacaggtatt ttatcatgtt
     5401 gaaaatcaga tttatccaaa acacttactg ctaccaattt ccatgaaagt tcatcagatt
     5461 ctagcaattt atcaagaact ttttccatat ttttcgttac tgttaacagt aaaattttct
     5521 tgctattttt tagattaaaa aacacatgct tccaatattt ttttactaag aaatttaaca
     5581 agtataagg
//
LOCUS       DAVLYV010000099         5290 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLYV010000099 DAVLYV010000000
VERSION     DAVLYV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.099"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            200..409
                     /locus_tag="VYO70_001898"
     CDS             200..409
                     /locus_tag="VYO70_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1714063.1"
                     /translation="MSQQHKKWIRLVKDKLNSEGMTQTHLARACGVKKSTISELLKYG
                     KGSDKLKNRVCDVLRIDETWVELGE"
     gene            413..1123
                     /locus_tag="VYO70_001899"
     CDS             413..1123
                     /locus_tag="VYO70_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEV1714064.1"
                     /translation="MNEIFNFHGQEVRTLTIDNEPWFVGKDVADILGYSKARNAIALH
                     VDEEDALKQGIPTSGGTQDMLIINESGLYSLILSSKLPQAKEFKRWVTSEVLPAIRKQ
                     GGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAQSLLKKRKAR
                     VVACLGGIDSPAYADKTFAQSVFRQAEIDFKDHFNISRYDLLPKKFADAALAYWMTWE
                     PSTNTKMKIMKLNSFDDV"
     gene            1137..1394
                     /locus_tag="VYO70_001900"
     CDS             1137..1394
                     /locus_tag="VYO70_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1714065.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            1480..1800
                     /locus_tag="VYO70_001901"
     CDS             1480..1800
                     /locus_tag="VYO70_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714066.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQVRNHAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPELLALPEPKQSA
                     IRFL"
     gene            1816..2112
                     /locus_tag="VYO70_001902"
     CDS             1816..2112
                     /locus_tag="VYO70_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714067.1"
                     /translation="MEELIESLDNLIMIVKELEGRESTSRHFITIWENDYKNLLRVKE
                     YLTDYEKLAKDYRDVELKNKLLKIEKMELEGRYIYEDMRMKYRANRRKWGARYV"
     gene            2105..2938
                     /locus_tag="VYO70_001903"
     CDS             2105..2938
                     /locus_tag="VYO70_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1714068.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALDKELDKDKELDKD
                     IEINNNKVMISSSLSENLKNSGIHLTNKSHQQLLEYVGLDGMSFDMLNRAVEKTSGSH
                     KPSFNYLIAILESWKKKGFTSIEQVDEDDRKYKEGKNYNHSRQQNNEISEQEVKDEWG
                     F"
     gene            2926..3084
                     /locus_tag="VYO70_001904"
     CDS             2926..3084
                     /locus_tag="VYO70_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714069.1"
                     /translation="MGILELIEQFEDEFYPISDEKKSLLIKQPLSTVTACLSEMASWH
                     ECGGRLSW"
     gene            3078..3848
                     /locus_tag="VYO70_001905"
     CDS             3078..3848
                     /locus_tag="VYO70_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1714070.1"
                     /translation="MVDNVFDEIALSYHRNTEQQKELCDKHNIPLIKILRTESVVCRM
                     CESERIHEENQARVNELADAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LVFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAMVKALSEYTKEIAIFINVTDLLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLTS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            3863..4057
                     /locus_tag="VYO70_001906"
     CDS             3863..4057
                     /locus_tag="VYO70_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714071.1"
                     /translation="MFILRHGTREDKPFLRSVVIGVTGLDISCSEEKKAMRFVSRGAA
                     VQVGKALRGSFGNFYPVEVE"
     gene            4411..4575
                     /locus_tag="VYO70_001907"
     CDS             4411..4575
                     /locus_tag="VYO70_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714072.1"
                     /translation="MVGVTYQEIHLFVEFLKEQYGQGRPDYIEALNDLDGLVEVSYRE
                     AIERFLEDEL"
     gene            4565..4774
                     /locus_tag="VYO70_001908"
     CDS             4565..4774
                     /locus_tag="VYO70_001908"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714073.1"
                     /translation="MNYDKRTVIDGLKRTIEQNEEKIIEYSKPCDARKRRIRALERDL
                     LKKKNKELRRKAEELEDDGRVKEKS"
     gene            4746..5063
                     /locus_tag="VYO70_001909"
     CDS             4746..5063
                     /locus_tag="VYO70_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714074.1"
                     /translation="MMEELKKKVNAVYNWTVEDGKPQPPQQDLPQAVKDRADYFWEMA
                     EDGMTFMGAMECIFADEKPTDYDLGATKGWLPKSKEFDDWVGYSPGMSQVVIAVYLIY
                     GGN"
     gene            5065..>5290
                     /locus_tag="VYO70_001910"
     CDS             5065..>5290
                     /locus_tag="VYO70_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714075.1"
                     /translation="MNKQELIEKYKKLEGVWNAEGAELARQIFLQDLEQLDKPKPVKV
                     PQCVAEYIEFKKKNNFHVYGAMRVIEDHYDK"
BASE COUNT         1782 a          809 c         1264 g         1435 t
ORIGIN      
        1 aataaagaca tagagaaaag acctactaaa gtaagtttta cctatagaaa acggacgcca
       61 atcagttttg taaggcttta ttttttagtt gtctagttcg ctaactcttt agcttacgaa
      121 tactatttta aaactagttt tagaatttgt caagtagttc gctaactttt tagataattt
      181 ttttaaaagg aggtcaggga tgagccaaca acataaaaaa tggattcggt tagttaagga
      241 taaactgaat tcagaaggaa tgacacaaac acaccttgct cgtgcttgtg gagtgaagaa
      301 atctaccatt tcagaattat tgaaatacgg taaaggtagc gacaaattaa agaaccgagt
      361 ttgcgacgtt ttaagaattg acgaaacttg ggttgagtta ggagagtagt atatgaacga
      421 aatttttaat tttcacggac aggaagtccg tactttgaca attgataacg agccttggtt
      481 cgtcgggaaa gatgtcgcag acatcctagg atatagtaag gctagaaatg cgattgctct
      541 tcatgttgat gaagaggacg ccctaaaaca gggcatccct actagtggtg gaacacagga
      601 tatgttaatc atcaatgaat ctggtctcta ctctctcatt ttatccagca aattgcctca
      661 agcaaaagag ttcaagcgtt gggtgacatc agaggtcttg ccagctatta gaaaacaagg
      721 cggatttatc cgagaggact tggatgaaga tgctttcatc gctctgttta ctggacagaa
      781 gaagttgcgt gagcaacaag cgactatgct ggaagatatt gactacctca agagtgagca
      841 accgattcat cctagctatg ctcaatcatt actgaagaag cgcaaagctc gtgtcgtggc
      901 ttgcttaggt ggtattgata gtccagctta tgcggataag actttcgctc agtcagtctt
      961 tagacaagct gagattgatt tcaaagacca cttcaacatt agtcgctatg atttgctacc
     1021 gaaaaagttt gcagatgcag ccttggccta ctggatgacg tgggagccaa gcacaaacac
     1081 taagatgaaa atcatgaaat taaactcatt tgatgacgtc tagaaagggg aagaagatgg
     1141 ataatgttct actttcactg tctgaatgga ttaaatctat tatcaaggac acaatcacaa
     1201 ggctagtcga aatagagaaa gatagtgacc actatccaga gttgatggat gtgaacacta
     1261 cctgcgaatt tctaggaatt aagtatgcca ccttttcaga taattatcgt tacttaaagg
     1321 gatttccaaa ggaattacct ggtaagaaat ggtcaaaaag agccatcaaa gaatggctct
     1381 ctaatcaaat ataataactt tactaaaagg cttctggaca aggtcttagc aaaattattt
     1441 gactatatta tagcacaaaa agaggataag gagataaaaa tgtttgaacc accgatttta
     1501 gaccagctga tgggggttgg agccttgctg cttggatttg caggggcttg ccgtcatatc
     1561 aaattgcagg aacaacgcaa ggaagaagaa agacgagaag agcaagaatt tgcgtctatg
     1621 attatccaag tgcgtaatca tgcatatgaa cgtggtagag aggacaaatg gcaagaaatt
     1681 cgcaagaata ttcgcagaga gttcaaagga ttcacatatg acaacgaacc gcctgtagga
     1741 ttgcgccctg agctgttagc tttgccagaa cctaaacagt ctgcaatcag atttttgtaa
     1801 tgaggaggtc aggaaatgga agaattgatt gaatcgctgg ataacctgat tatgattgtt
     1861 aaagaactgg aaggaaggga atcaacttca agacatttta ttacgatatg ggaaaacgat
     1921 tataaaaatc tattacgagt caaagaatac ctaacagact atgaaaaact agctaaggac
     1981 tatcgtgatg tggagcttaa aaataagctg ctaaagattg aaaaaatgga gctggaaggc
     2041 aggtacatct atgaggatat gcgaatgaag tatcgcgcta accgtaggaa gtggggtgct
     2101 aggtatgtct gaaattaagt ggataaaaat cacaaccgat atttttgacg atgaaaagat
     2161 ttgcctgatt gatgccttgc ctgatcctga tgccatctta gtgatatggt tcaagatttt
     2221 gacacttgct ggaaaacata acagtaatgg tttgttgatg atgactgata aggttcacta
     2281 tacagatgaa atgttagcta ctatttttcg tagaccattg aatacagtaa gaatggctat
     2341 tggagttttt gaacagtttg ggatgattga gattatcgat ggtattatta gcttgccaaa
     2401 ttgggaaaaa catcaaaacg ttgacggaat ggagaaaatc aaggaacaga cacgtaaccg
     2461 tgtagccaaa taccgtaaaa aacaaaaaaa tcttgctctt ggtaacgtta caggtaacgt
     2521 tacagtaacg gacggtaacg cactagataa agaattagat aaagataaag aattagataa
     2581 agatatagag ataaataata ataaggtgat gattagttcc agcctctctg aaaatttgaa
     2641 aaatagcgga atccatctaa ctaataagtc acatcaacag ttgcttgaat atgtaggact
     2701 tgatggaatg agttttgata tgttgaaccg tgcagtcgag aaaacttcag ggtcacacaa
     2761 acctagtttc aattatctaa tagctattct tgaaagttgg aaaaagaaag gcttcacatc
     2821 gattgaacaa gtggatgagg acgaccgtaa atataaagag ggtaaaaact acaatcattc
     2881 aagacaacaa aataatgaaa tatcagaaca ggaggtcaag gacgaatggg gattttagaa
     2941 cttatcgagc aatttgaaga tgaattttat ccgataagcg acgaaaagaa atctttgctt
     3001 ataaaacaac ctctttctac tgtcactgcc tgcttgtctg aaatggctag ctggcatgaa
     3061 tgcggaggtc gtctgtcatg gtagacaatg tgtttgatga aattgcctta tcttatcaca
     3121 ggaatacaga acaacagaaa gagctttgcg acaagcataa tattcctttg ataaaaatat
     3181 tgcggactga gagtgttgta tgccgcatgt gtgaatctga gcggattcat gaggaaaatc
     3241 aagcaagagt gaatgaattg gccgacgctg agaatgagcg agagaggaaa tactatcttg
     3301 agaagttttc tctttatgat gaggttttga aaaatgcgac tttggacaat tttgaaacac
     3361 caactgaaaa agaagcagaa aagctagttt ttgcaaagag gatttgtcgt gagtggtctg
     3421 agggtgctag gaacaacatc gtgttacaag gagaagctgg aacaggtaag agccatttgg
     3481 cctttgcgat ggttaaggct ctatctgagt acacgaaaga gattgctatc ttcatcaacg
     3541 tgacggactt gttgatgaag attaaagctg attttagtca ggaggagttt ctggtcaata
     3601 aaattgccag tgctaagttc ttggttttgg atgatttggg aatggaaaag gatagcgaat
     3661 ggtcgtttac tattctctac aatatcctga ataagcgttc aaatacaatc attaccacca
     3721 atttgacttc tgctgatatt cagaaaagat atggcagacc ctttatgtcc agactgatga
     3781 agggtgtgga taaagaccat ttaatggttt tcaacgactt gacaaacaag cggaagcaat
     3841 atttttagaa tggaggtggc tgatgtttat tttaagacat gggacaagag aggataagcc
     3901 gtttctgagg tccgtagtta ttggtgtgac tggcttggac atttcatgtt cagaggagaa
     3961 gaaagccatg cggtttgttt ctcgtggggc agccgtacag gttggtaagg ctttgagggg
     4021 ttcctttggg aatttttatc ccgttgaggt ggagtgatgt tagagcttta cttcgtctac
     4081 aacgggcact gcaagtttta ccttggaacg tttgacaatg tcgatgatct cattgaacag
     4141 atggaagatc atcagtgggt tttctcggct atcactcatc caagatttca gaagcacatt
     4201 ggtcagcgga cgacacggtt tgactacggt tcgaaggatt gttactattt agcgactttt
     4261 tcaggaggag aaaaaaatga ttgaacttat taaagaattt ggaatggcta ttctgtggtt
     4321 atttctcggc tatttagtcg gggaacgtgc agcaagaaag gaaaagaaag atgatcaata
     4381 acgttacatt tttagtggga ggtaagaaat atggttggag taacctatca ggaaattcat
     4441 ctctttgttg aatttttgaa agagcagtat ggacaagggc gtccagacta tattgaagcc
     4501 ctgaacgact tagacggtct ggtggaagtc tcctacagag aagctattga aagattttta
     4561 gaagatgaat tatgataaac gaacagtcat tgatggactg aaacgcacaa tcgagcaaaa
     4621 cgaagagaag ataatcgagt attcgaagcc gtgtgatgca cgcaagagac ggattagagc
     4681 gctggagcgc gatttgttga agaaaaagaa taaagaatta agacggaaag cggaggagtt
     4741 ggaagatgat ggaagagtta aagaaaaaag ttaatgcagt atacaactgg acggtagaag
     4801 acgggaagcc gcaacctccc cagcaagatt taccacaagc agtgaaagac cgggcggact
     4861 atttttggga aatggcagaa gatggtatga cgtttatggg agcgatggaa tgcatcttcg
     4921 ctgatgaaaa gcctacagac tatgatttgg gagctactaa gggttggttg ccaaaatcta
     4981 aggagtttga tgattgggtt ggctattcgc caggaatgtc tcaggtagtt attgcagttt
     5041 atttgattta tggaggaaac taagatgaat aagcaggaat tgattgagaa atataaaaaa
     5101 cttgagggtg tatggaatgc tgaaggagca gaactagctc gtcaaatttt tctgcaagac
     5161 ttggaacaac tagataaacc aaaaccagtc aaagttccgc agtgtgtggc ggaatatata
     5221 gaatttaaaa agaaaaacaa ttttcatgtt tacggtgcaa tgagagtaat tgaagatcat
     5281 tatgataaga
//
LOCUS       DAVLYV010000100         5240 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLYV010000100 DAVLYV010000000
VERSION     DAVLYV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.100"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(88..996)
                     /locus_tag="VYO70_001911"
     CDS             complement(88..996)
                     /locus_tag="VYO70_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1714076.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            1060..1329
                     /locus_tag="VYO70_001912"
     CDS             1060..1329
                     /locus_tag="VYO70_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1714077.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            1329..1709
                     /locus_tag="VYO70_001913"
     CDS             1329..1709
                     /locus_tag="VYO70_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1714078.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            1798..2025
                     /locus_tag="VYO70_001914"
     CDS             1798..2025
                     /locus_tag="VYO70_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714079.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(2065..2790)
                     /locus_tag="VYO70_001915"
     CDS             complement(2065..2790)
                     /locus_tag="VYO70_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HEV1714080.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVEVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            2952..4793
                     /gene="typA"
                     /locus_tag="VYO70_001916"
     CDS             2952..4793
                     /gene="typA"
                     /locus_tag="VYO70_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1714081.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            4813..5067
                     /locus_tag="VYO70_001917"
     CDS             4813..5067
                     /locus_tag="VYO70_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1714082.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         1546 a          967 c         1157 g         1570 t
ORIGIN      
        1 gggatttacc cactacaaat attatagagc ctaaaaagaa ccaatgtgca ggttgttgct
       61 agcctacata ttggttctta tcatttctca actatcaaac tgaacttctt ctagtagata
      121 gtcaataaag cgttcgccca tcttagatag gctggttttc tcatgctgga tatagaccag
      181 ctcaatcggg tcatcaatat ccagtgggat agaaacgata ttgtctccgt ttaggttgct
      241 gttcaaaatc cctgtcgcaa tggtataacc atccaaacca atcaagagat taaagagggt
      301 ggcacggtca ctgaccacaa tggatttctt gtggtgttct tgagaaagaa tctcttctga
      361 aaagtagaag gagttgtgcg tcccttggtc atagctgagg taagggaaat tctccaaatc
      421 agacagtttc accttgtctt tctttgccag agggttggtc ttgctgacaa agatatgcgg
      481 ttgcgctgtg aagagatggt gggctagcag gtgattgtca tccagcatct tggttaaaac
      541 atcacggttg taactgttta agaagaggac cccgacctca ctgcggaagt tcttgacgtc
      601 gtcgataatc tcccaagtcc gagtttcacg aaggaagagt tcgtatttct ccatatcgct
      661 tttcttgagc aaagagacaa aggcattgac cacaaaggca tagtgttgag acgaaacgct
      721 aaagagttcg cggtgggcaa caggattttt atagcgttcc tccagaagct gggtctgctc
      781 gacaacctga cgggcataag agagaaactc catcccatca cgggtcaagg taataccctt
      841 gggattgcgg ataaagattt caatgcccat ttcattttcc aaatctcgca ctgcattgga
      901 gagactgggc tgagtgataa agagttgctt ggctgcctca ttcatagagc cagtttcgac
      961 gattttgata atataatgta attgttgaat tctcatgttt ttattgtacc acacttgcct
     1021 tagaatcgcc aaagaagata ggaaaataag gagcattgtg tgagtctctt cttgaaaaaa
     1081 acacaaaatt ttgctagaat gagtcttatg aaaacattct atgatgtgca gcaattcctc
     1141 aaacgatttg gtattattgt ttacatggga aaacgcttat atgatattga actgatgaag
     1201 ttggaactat ctcggattta cgatgcaggg ttgatggata aattagacta tctagaggca
     1261 gaagcagttc ttcgcagaga gcacaaggta gaattggatt atattgagaa aaatggagaa
     1321 aagaactaat ggttacttgg attttgtggg cacttatact agcaatgttg gcgtggatgg
     1381 gctttaacta tcttcgtatt cgtcgtgcgg ctaaaattgt ggacaatgag gagtttgaag
     1441 ccttgattcg tacgggtcaa ttgattgatt tgcgcgaccc agcagaattc cacagaaaac
     1501 atatccttgg tgcacgcaat attccttcaa gtcagttgaa aactagtctt gcagcccttc
     1561 gtaaagataa acctgtcctt ctctacgaga accaacgtgc gcaacgagtt acaaatgcag
     1621 ctctttactt gaaaaaacaa ggtttttctg agatttatat cctttcttat ggcttggatt
     1681 cttggaaagg gaaagtgaag actagctaac cttatttcca aattagtcga atgataggat
     1741 gaagctattg ttttatagta taattaattt gtataaaatt taaggagttc ttagaacata
     1801 aaagaaaaga agaaaaatcc actttttgtg ggtatcttgt cgattattct tggtttgtta
     1861 tttccaattg taggcctgat tttgggtatc ataggattgg tcttggctat ttcataccaa
     1921 aaagagtcac aattagacta taaaatagaa aagattctta atatcttagg aattgtgatt
     1981 tctgtagtta actggattgt agcaatcgcc ttaatttttc ggtaaataaa agaaaaaagc
     2041 tttagtttta aagctttttt tattttatcg actcatctct aaatagtttt taatgatttg
     2101 taactctttt tggttcaaag ggcggtagtt accttctgct aaatctgggt tcaatgtgaa
     2161 gtccccaaat tggattctct tgaggctagt taccttaaca ccaaccgaga ggaacatttt
     2221 cttgatttga tgaaattttc cttctgaaat ggtgatagag gcttgactga gggaaggact
     2281 tgcagataga atctctagtt ttgcgggttt acagacagta ccatctaaaa agacaattcc
     2341 cttttgaaag gcttggatat ggtcaggtgt tagaagtcca ttaacctcaa cttgataagt
     2401 cttatcgaca tgatattggg gatggaggag ttggaagccc aagggaccgt tatcggtcaa
     2461 gagaaggagt cctgttgtat ctcggtccag tcggccgacg gcatagagct tgtcagactg
     2521 gatgtcagat ggaagcaggt ccatgacggt cggaagttcc ttgtctttgt tggctgtaac
     2581 gacaccagca ggtttatgaa gcataagata ggtgtgttca tagccttgaa tgattcgatc
     2641 ctgaaaaagg agttcttgta gtcctgtatc gatattttga gctagggagc gggctgggca
     2701 accatcgact agaatttccc ctctgaggag tgcttgcttc atggcctttc ggctaacttt
     2761 ttcttgggct aataaattat ctaaacgcat actgtgccta tcttatcata agtcgcttgc
     2821 tttgaaaagg cttgacgtga aaagaaaagc ggagtgaaaa catttacact tgcttgagtt
     2881 atgttattta tttgaaatta tggtataatc gttcagttag aaaataaatt ttgaatatta
     2941 tagaggaaat catgacaaaa ttaagagaag atatccgtaa cattgcgatt atcgcccacg
     3001 ttgaccacgg taaaacaacc ctggttgacg aattattgaa acaatcagaa acgcttgatg
     3061 cacgtactga attggcagag cgtgctatgg actcaaacga tatcgaaaaa gagcgtggga
     3121 ttaccatcct tgctaaaaat actgccgttg cttacaacgg aactcgtatc aacattatgg
     3181 acacaccagg acacgcggac ttcggtggag aagttgagcg tatcatgaaa atggttgacg
     3241 gtgttgtctt ggtcgtagat gcctatgaag gaaccatgcc acaaactcgt ttcgtattga
     3301 aaaaagcctt ggaacaagac cttgtcccaa tcgtggttgt taacaaaatc gataagccat
     3361 cagctcgtcc agcagaagta gtggatgaag tcttggaact tttcatcgag cttggtgcag
     3421 atgacgacca gcttgatttc ccagtggttt atgcttcagc gatcaacgga acttcttcat
     3481 tgtcagatga tccagctgac caagaagcga ctatggcacc aatctttgac acgattatcg
     3541 accatatccc agctccagta gataactcag atgagccttt gcagttccaa gtgtcacttt
     3601 tggactacaa tgactttgtt ggacgtatcg gtatcggtcg tgtcttccgt ggtacagtta
     3661 aggttgggga ccaggttacc ctttctaaac ttgacggtac aactaaaaac ttccgtgtta
     3721 caaaactctt cggtttcttt ggtttggaac gtcgtgaaat ccaagaagcc aaagcgggtg
     3781 acttgattgc cgtttcaggt atggaagaca tctttgtcgg tgaaaccatc actccgacag
     3841 atgcagtaga agctcttcca atcctacaca tcgatgagcc aactcttcaa atgactttct
     3901 tggtcaacaa ctcaccattt gctggtaaag aaggtaaatg ggtaacttct cgtaaggtgg
     3961 aagaacgctt gcaggcagaa ttgcaaacag acgtttccct tcgtgttgac ccaactgatt
     4021 caccagataa atggactgtt tcaggacgtg gagaattgca cttgtcaatc cttatcgaaa
     4081 caatgcgtcg tgagggctat gaacttcaag tatctcgtcc agaagttatc gtaaaagaaa
     4141 tcgacggtgt taaatgtgaa ccatttgaac gtgtacaaat cgacactcca gaagaatacc
     4201 aagggtctgt tatccaaagc ctttctgaac gtaaaggtga aatgttggat atgatttcaa
     4261 ctggtaatgg tcaaactcgt ttggtcttcc ttgttccagc gcgtggtttg attggatact
     4321 caactgagtt cttgtcaatg actcgtggtt acggtatcat gaaccatacc ttcgaccaat
     4381 acttgccatt gattccaggg gaaattggtg gacgtcaccg tggtgccctt gtttctatcg
     4441 atgctggtaa ggctacaact tactcaatca tgtctatcga agaacgtggt acgatctttg
     4501 tcaacccagg tactgaggtt tatgaaggaa tgatcatcgg tgaaaactct cgtgaaaatg
     4561 acttgacagt taacatcacg aaggcaaaac aaatgaccaa cgttcgttca gctactaagg
     4621 accaaacagc tgttatcaag acgcctcgta tcttgacact tgaagagtct cttgagttct
     4681 tgaacgacga tgagtacatg gaagtaacgc ctgagtctat ccgtttgcgt aaacaaatcc
     4741 ttaacaaagc agagcgtgag aaagctaaca agaagaaaaa atcagctgaa taagagctag
     4801 aaagagagaa agatggtcta tttagtccta ggaattttac tgctcctact ctatgtattt
     4861 gcgacaccag aaagcattaa agggactgtc aatatcgtcg ctatggtatg tattttagtg
     4921 gcactcttga ttttattggt tctatctttt ctgaaaattt ttcaattacc aacagaaata
     4981 ttcctagcaa tagccatgtt gatcctagct tactttagtg ttagagacat cacactcatg
     5041 ccagtcaaaa aaagtaaaag aagataaaaa gagagttttg gctctctttt ttgctaggaa
     5101 ttttacaaga ttgcaattta tttttgagtt atcaccatct cttttgtttg gataatatga
     5161 atgggaaaaa cttgaataaa attctttaat ggcatctatt ttatatttgg ttagtactgg
     5221 ttgcatatta ttttgaaata
//
LOCUS       DAVLYV010000101         5212 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLYV010000101 DAVLYV010000000
VERSION     DAVLYV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.101"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            108..1631
                     /locus_tag="VYO70_001918"
     CDS             108..1631
                     /locus_tag="VYO70_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714083.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNTFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1615..3336
                     /locus_tag="VYO70_001919"
     CDS             1615..3336
                     /locus_tag="VYO70_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714084.1"
                     /translation="MKEFYKKRFALTDRGARNLSKATLASFFVYCINMPPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVKGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFNKVSIVFQDVVLFNQSIMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            3347..3931
                     /locus_tag="VYO70_001920"
     CDS             3347..3931
                     /locus_tag="VYO70_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1714085.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            3931..4626
                     /locus_tag="VYO70_001921"
     CDS             3931..4626
                     /locus_tag="VYO70_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1714086.1"
                     /translation="MPTFSSNAPFNPNPISKILVIFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGISF
                     KNPVKYLEYVSMPLLIISSNISDDIAKAAETKAIENPIAKTRYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            4614..>5212
                     /locus_tag="VYO70_001922"
     CDS             4614..>5212
                     /locus_tag="VYO70_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714087.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLKIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSINVLAKMLKILKEKGISIIVAEHRI"
BASE COUNT         1866 a          690 c          984 g         1672 t
ORIGIN      
        1 ttctagataa gttaataatt aattcaaact tatccggtgc agagagtata gcattaaaat
       61 ctgttattac actaacaagt ggagcgatat tttattttgt ctcaggaatg ttttcacata
      121 tcttgggatt taggcttgaa acaaatttaa gaaaaagggg aatcgatggt ctggaaaaag
      181 caagttttag gttctttgac ttaaatccat ctggtcaaat aagaaagatc atagatgata
      241 atgcagcaca aactcatcag gtggtagcac acatgattcc cgatagttct caggcaataa
      301 tcacacccgt acttgtactt gcacttggct ttatagtaag tataagggtt ggcataattt
      361 tgcttgctct tactataatt ggtggcttaa ttttaggggc aatgatgggc gagcaagaat
      421 ttatgaagat ataccaagaa tccctatcta aactaagtgc tgaaactgtt gagtacgtga
      481 gaggaatgca agttgtaaaa atatttaaag caaatgtaga gtcttttaaa agcttttata
      541 aggcgataaa agattactca aagtatgctt atgattattc cctatcttgt aaaaggcctt
      601 atgttttgta tcaatggtta ttttttggac tgattgcaat tttaattatt cctatagttt
      661 attttatgac tagcttagct agcgcaaagg tgattttact tgagcttatc atgattttat
      721 ttttatcagg agttctcttt gtttcattca tgagaatgat gtggtactcc atgtatattt
      781 ctcaaggaaa ttatgcagta gatactttag aggcgcttta cgaagatatg caaaaagaca
      841 aattagtgca tggtaatgtc aatactttta aaaactataa tatagaattt gagaatgtta
      901 gctttgctta taatgataaa gctgtcattg aaaatttatc ctttaattta gaagaaggaa
      961 agtcctacgc acttgtcggt tcatctggat caggcaaatc aacagtagca aaacttatat
     1021 caggttttta caatgttaat aaaggaagca taaagatagg cgggatagca ataagtgaat
     1081 attctgacga agccttaatt aaagccattt cctttgtttt tcaagattca aaattattca
     1141 agaagagcat ttatgataat gtagctttag ctaataaaga tgcgacgaaa gatgacgtta
     1201 tgagagcctt aaaattagca ggatgcgatt taatattaga caaattccca gaaagagaaa
     1261 atacaatcat agggtcaaaa ggtgtttatt tatccggtgg agaaaaacaa agaattgcaa
     1321 ttgctagagc aattttaaag gattccaaaa ttattattat ggatgaagca tcagcatcta
     1381 ttgacccaga taacgagttt gagttgcaaa aagctttcaa aaatcttatg aaggataaaa
     1441 cagttatcat gattgcacac aggctatcta caattaaaga ccttgatgaa attattgtta
     1501 tggatagtgg aaaaattata gaaagagggt ctgacaaaga attaatgtca aaagatacaa
     1561 ggtataagag cctgcaagag atgtttaaca gtgcgaatga atggagggtt tcaaatgaaa
     1621 gagttttata aaaaaagatt tgctcttaca gatagaggag caagaaattt aagtaaagca
     1681 acactggctt catttttcgt ttattgtata aacatgcctc ctgccatatt acttatgatt
     1741 tttgctcagg aagttttgga aaatatgggc aaaagcaatg gcttttatat agtattctca
     1801 gttttgactt tgatagcaat gtatattttg ctttctatcg aatacgataa attatataac
     1861 acaacctatc aagaaagtgc agatttaaga ataaggacag cggagaattt atcaaaatta
     1921 cctctatctt acttttctaa acatgacatt tccgacattt cacaaacaat catggctgat
     1981 attgaaggca tagagcatgc aatgagccac tcaataccaa aggtgggcgg catggtactg
     2041 tttttcccat taatatctgt aatgatgcta gcgggcaatg tcaagatggg tttagctgta
     2101 attattccat ctattttaag ctttatattt atacctttat ctaaaaaata tcaggttaag
     2161 ggacagaata gatattatga tgtcttaaga aaaaactcag aaagctttca agaaaatatc
     2221 gaaatgcaaa tggagattaa agcatataat ttatcgaagg atattaaaga tgacttatat
     2281 aaaaaaatgg aagatagtga gagagtacac ttaaaggcgg aagtaactac aattttaact
     2341 ttgtctatat cttcaatatt tagctttata tctcttgctg ttgtgatatt tgtcggcgta
     2401 aatctaatta ttaataaaga gataaattct ctctacctta taggatattt actagctgct
     2461 atgaagataa aagactcttt agatgcatct aaagagggct tgatggaaat attttattta
     2521 tcgcccaaaa tagaaagatt aaaagaaatt caaaatcaag atttacaaga aggcgatgac
     2581 tatagtttaa aaaaatttga tattgatcta aaagatgttg agtttgccta caataaagac
     2641 gcaaaagttt taaatggtgt aagttttaaa gctaagcagg gagaggtcac tgctttggta
     2701 ggtgcaagtg gctgcggtaa aacaactatc ttgaaactta tatcaagact ttatgattat
     2761 gacaagggac aaatcttaat cgatggcaaa gatataaaag aaatatcaac agaatctctt
     2821 tttaataagg tctctatagt tttccaagat gtggttctct ttaatcaaag cattatggaa
     2881 aatattagaa tcggaaagca agatgcaagt gacgaagagg ttaaaagagc agcaaaactt
     2941 gcaaattgca cagattttat agaaaaaatg gataaaggtt tcgatacagt tattggtgag
     3001 aacggagctg agctatcagg aggagaaaga caaagattat caatagccag agccttctta
     3061 aaagatgcgc cgatattgat cttagatgag ataacagcaa gccttgatgt taacaacgag
     3121 aaaaagattc aagagtcttt aaataattta gttaaagata aaactgttgt aatcatttca
     3181 catagaatga aatccataga aaatgcagac aagatagtag ttcttcaaaa cggaagagta
     3241 gaaagcgaag gtaagcatga agagctttta caaaaatcaa aaatttacaa aaatttaata
     3301 gaaaagacaa aaatggcaga agaatttatt tattaggagg actacaatgg ataataaaaa
     3361 attaaaagta aaagatttag taagcatcgg tgtttttggc gtaatttatt ttgccttcat
     3421 gtttggagtt ggtatgatgg gcttgattcc aatattgttc ttaatatacc cgacagtatt
     3481 agccatagtt gcaggaactg ttgttatgtt atttatggct aaggttcaaa agccatgggc
     3541 actatttata tttggtatga tatcaccact tgtgatgttt gcagctggtc atacctacgt
     3601 agttgtggtt ttatcactta tagtaatgat aatagcagaa ttaattagaa agattggtaa
     3661 ttataattca tttaaataca atatgctttc ttatgcaatc ttcagcacat ggatatgtag
     3721 ctctttaatg caaatgcttt tagcaaaaga aaaatatatg gagtggtctt tgatgacgat
     3781 gggaaaagat tatgttgatg tattagaaaa gttaataact tatcctcata tggctttagt
     3841 agccttaggt gctttcttag gaggaattct tggagcatat ataggcaagg ctctattgaa
     3901 aaaacacttt gaaaaagcag gcattgtata atgcctactt tttcttcaaa tgctcccttt
     3961 aatcccaatc cgatctctaa gatattagtc atatttatca caggcttaac tgttatgcat
     4021 agcataaaca tccgttttga gctggcaatt gtttgtctta ttggtatttt gctttatttg
     4081 aatggataca aaaaaacact tttcaaatgg attatattgt gtgggatact ttactccttg
     4141 cctaatttta tggtgctatc tgaattaaac cctatagtta agatgttttt gagtatacct
     4201 attgttatta gaatgtttat tttaccattt atggcagcaa gctttatgat aaagacctcg
     4261 gatgtaggcg caataatttc atcgatggat aagcttaaga tttcaaagaa tgtatccata
     4321 cctattgcgg ttatgtttag attcttccca tcttttaagg aggagaagaa aaacatcaaa
     4381 atggctatga gagtaagagg gataagtttt aaaaacccag tcaaatatct tgaatatgtt
     4441 tctatgccac tactcattat atcatctaat atatcagatg acattgcaaa agcggcagaa
     4501 acaaaggcaa tagaaaatcc aattgccaag accagataca ttcgcgtaaa gatacagcta
     4561 attgattttg tttatgtttt agcggttgct ggacttattg tgggaggctt aatatggttg
     4621 aaataaaaaa tttaagtctt gattatggtg aagagcatat attagatgat atatcactat
     4681 ccatagccga gggagagtgc gtgctattta caggaaaaag tggaaatggt aagtcatctt
     4741 taataaattc aatcaatgga ctagctgtaa ggtatgataa cgcaaagaca aagggcgaaa
     4801 taattattga tggtaagaat ataaaaaatt tggaacttta tcaaatctca atgcttgttt
     4861 caactgtttt tcaaaatcct aagacatatt tttttaatgt caatacgaca ttagaattat
     4921 tattttattt ggaaaatatc ggtcttgcaa gagaagagat ggacaggcgt ttgaaggata
     4981 tgcttaagat attcccgata aaaaatcttt tgaacagaaa tatatttaat ctatccggcg
     5041 gtgaaaaaca aattctttgc attgcagctt cttatatagc aggtacaaag attatagtta
     5101 tggatgagcc ttcatcgaat ttagatatta aaagcataaa tgttttggca aagatgctaa
     5161 agatattaaa agagaaaggc ataagcataa ttgttgcaga gcatagaatt ta
//
LOCUS       DAVLYV010000102         5176 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLYV010000102 DAVLYV010000000
VERSION     DAVLYV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.102"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..890
                     /locus_tag="VYO70_001923"
     CDS             <1..890
                     /locus_tag="VYO70_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEV1714088.1"
                     /translation="ILESTPYHLSIDILEDPSQTEISLMTKLVNDYRWAYAESPSDII
                     VTLFALRYVYHNIKVLLKSKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFM
                     VREVESIWNEYETFNNIRVLDVGADLAYFKHLKLLSNELDEVLSQVIVEMIDFYNIIT
                     VKRGLSQNKSHGDILQLLSDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYEL
                     KMQDATISSSELEFLCDLLLYKTLDQGRYNVEGPLVLARYLLGCEFEVKNLRMIISAL
                     QNTIPFESIKERIRPHYGS"
     gene            880..1200
                     /locus_tag="VYO70_001924"
     CDS             880..1200
                     /locus_tag="VYO70_001924"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEV1714089.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            1263..3038
                     /locus_tag="VYO70_001925"
     CDS             1263..3038
                     /locus_tag="VYO70_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEV1714090.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     AFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFANVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGLPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGTYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            3039..4424
                     /locus_tag="VYO70_001926"
     CDS             3039..4424
                     /locus_tag="VYO70_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEV1714091.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLPSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            4447..5058
                     /locus_tag="VYO70_001927"
     CDS             4447..5058
                     /locus_tag="VYO70_001927"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEV1714092.1"
                     /translation="MVRLNVKPTRMELNNLKERLTTAERGHKLLKDKRDELMRRFISL
                     IRENNQLRKEVESYLIDNLKSFAVAKSLKNSQMVEELFSIPSKEIELFVEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIYKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         1695 a          743 c         1074 g         1664 t
ORIGIN      
        1 ttatcttaga atcaactccc tatcatttat cgattgacat cttagaagat cctagtcaga
       61 cagagatttc gctaatgaca aaattagtca atgattatag atgggcctat gctgaaagtc
      121 cgtctgatat aattgtgact ttatttgctt tacgatatgt ttatcataat atcaaagttt
      181 tattaaaatc taaggcggca attaagaaag atttttctaa attattaatt ccaataggga
      241 tttttgatat agaaagttta aaacatttag tttcttcctt acattcagat acacttcctg
      301 attttatggt tcgtgaagta gaatcaattt ggaatgagta tgaaactttt aataatattc
      361 gtgtacttga tgtcggagct gatctagcat attttaaaca tctgaaactt ttatctaatg
      421 agttagatga ggtactgtct caggttattg tcgaaatgat tgacttttat aatattatta
      481 ctgtaaaacg tggtttatct caaaataaga gtcatgggga tattttacaa ttactttcag
      541 atgaaggaag tatttctgct aaagaattta tatacattgt agaaaatcaa gaaatatttg
      601 tgtggttcaa taaaataaat ccaagcttag attcaatctt ttcaacttat gaattgaaga
      661 tgcaggacgc aacaatttca tcttctgagt tagaattttt atgtgattta ctattgtata
      721 aaactttaga tcaaggaagg tacaatgtag aggggccgtt agttcttgct agatatttat
      781 tgggatgtga gtttgaagta aagaatctca gaatgatcat atcagctctt caaaatacaa
      841 ttccctttga atcaataaaa gaaaggatac gcccacatta tggaagctaa taagtataaa
      901 attggcataa ttggtagccg tgatattatt ttaccattta gcatgattgg gtttgatata
      961 tttcctgcct accaagaaca agaagctata aatacactaa gaaaattagc tcaatctgat
     1021 tatggtgtca tttatatcac tgaagacatt gcttcaatga tattagatac aattcgccat
     1081 tatgattccc aagttgtgcc tgctattatt ttattaccga ctcataaaca aggtttaaat
     1141 ttaggattaa aacgtataga ggataatgta gagaaagcag taggacacaa tattttataa
     1201 taatgtacaa aattgtctgt aatattattc tataattttt ggacttagta aggagaataa
     1261 ctttgactca agggaagatt ataaaagtat cgggacctct agttattgca tcaggtatgc
     1321 aggaggctaa tattcaagat atttgccgtg taggtaagct agggttaatc ggtgaaatta
     1381 ttgaaatgag aagagatcag gcatctatcc aagtctatga agaaacatct ggtcttggtc
     1441 cgggagaacc tgttgttaca actggagaac ctctctcggt tgaattaggg ccaggattga
     1501 tttctcaaat gtttgatggc atacaacgcc cattagatcg atttaaattg gctactcata
     1561 atgattttct agttcgtggg gtagaagttc caagtttgga tagagatatt aagtggcatt
     1621 ttgattccac tatagcaatt ggtcaaaaag tgagtacggg tgatattctt ggaactgtca
     1681 aggaaaccga ggtagttaat cataaaatta tggttcctta tggagtatct ggagaagtcg
     1741 tttctattgc atctggcgat tttacaattg atgaagttgt atatgaaata aaaaaattgg
     1801 acggtagttt ctataaagga acgcttatgc aaaaatggcc tgtccgcaag gcgcgtcctg
     1861 tttctaaacg tttaattcca gaagaaccat taatcacagg tcaacgagtt attgatgcat
     1921 tctttccagt aaccaaaggg ggagctgcag cagttcctgg accgtttgga gcaggaaaga
     1981 cagttgtaca acaccaagta gctaaatttg ccaatgttga tattgttatt tatgtcggtt
     2041 gtggagaacg tggaaatgaa atgacggatg tactgaatga gtttcctgag ttgattgacc
     2101 ctaataccgg acaatcaatt atgcaacgga cagttctgat tgctaatact tcaaatatgc
     2161 ctgttgctgc tcgtgaggct tcaatttata caggaattac catggctgag tattttcgtg
     2221 atatgggcta ctctgtcgcc attatggctg attcaacttc acgttgggca gaagcgctac
     2281 gtgaaatgtc aggacgtcta gaagaaatgc ctggtgatga gggttatcct gcttatctgg
     2341 gaagtcgtat cgctgaatat tatgaaagag caggacgttc tcaggttcta gggcttccag
     2401 aacgtgaagg aacgattact gctattggag ctgtatcgcc acctggtgga gatatttcag
     2461 aaccagttac tcaaaacact ttacggattg tgaaagtttt ttgggggctt gatgctccgt
     2521 tggcacagcg acgtcatttt cctgcaatta actggcttac atcttattca ctatataaag
     2581 acagtgtggg cacttatata gatggtaaag agaagacaga ttggaatagt aaaataactc
     2641 gtgcgatgaa ctacttacaa cgggaatcta gtttagagga aattgttcgt cttgttggaa
     2701 ttgattctct gtctgataat gaacgactaa cgatggaaat tgctaaacaa attcgagaag
     2761 attatttgca acagaacgct tttgattcgg tagatacatt cacttcgttt gcaaaacaag
     2821 aagcaatgct aagtaatatt ctcacttttg ctgatcaggc aaatcatgct ttagagttgg
     2881 gttcttactt tacagagatt atggaaggta ccgtggcagt tcgagaccgt atggcgagaa
     2941 gtaaatatgt ttcagaagat agattagatg aaatcaaaat tatatcaaat gagattacac
     3001 atcaaattca tttgatatta gaaacaggag gtctataaat gagtgttata aaagaataca
     3061 gaactgctag tgaagttgtt gggcctctta tgattgttga acaagtaaat aatgtgtctt
     3121 acaatgagtt agttgaaatt caacttcata atggagaaat tcgtcgtgga caagttttag
     3181 agatccacga agataaagca atggttcagc tttttgaagg atctagtgga ataaatttag
     3241 aaaagtctaa aattcgtttt gctggtcatg cattagaatt ggctgtatct gaggatatgg
     3301 ttggtcgtat ttttaatggg atgggaaaac caattgatgg tggaccagat ttaattccag
     3361 agaaatattt agatattgat ggtcaagcta ttaatcctgt atctagagat tatccagatg
     3421 aatttattca gacagggatc tcctctattg atcatttgaa tactcttgta cgtggtcaaa
     3481 aattaccagt attttcaggt tcgggcttac ctcataatga attagctgct cagatagcaa
     3541 gacaagcgac tgttttaaat tctgatgaaa attttgcggt tgtatttgca gcaatgggta
     3601 ttacttttga agaagctgag ttttttatgg aagaactcag aaaaacagga gcgatcgatc
     3661 gttcggtttt atttatgaac ttggcaaatg atcctgcaat tgagcgtatt gcaactcccc
     3721 gcattgcttt aactgcggca gagtatctag cttttgaaaa agatatgcac gttctagtta
     3781 tcatgacgga tatgactaac tattgtgaag cgttacgtga agtctcggca gctcgccgtg
     3841 aagttccagg gagacgaggc tatccgggat atttatatac aaatttatca actctatacg
     3901 aaagggctgg tcgcttagtt ggtaaaaaag gttcggtgac acagattcct attttaacaa
     3961 tgccagaaga tgacataaca catccaattc ctgatttaac tggatacatt actgaagggc
     4021 aaattatttt gtcgcatgag ttgtataatc aaggttatcg tccaccaatc aatgttttac
     4081 cttctctctc tcgattaaaa gataagggat ctggagaagg taaaactcgt ggagatcatg
     4141 ctccaactat gaatcaactg tttgcagcct atgcccaagg gaaaaaggtt gaagagttag
     4201 cagtagtatt aggagaatcg gctttatctg atgtagataa attgtatgtg aggtttacaa
     4261 agcgttttga agaagagtac ataaaccaag gattttataa aaatcgaaat atagaagata
     4321 cgttgaatct tgggtgggaa ttactatcaa ttcttcctag aacagagtta aaacgtatca
     4381 aagatgattt gcttgataaa tacttacctt tggtagaagt ttaatccgga aatggagtga
     4441 ttatctatgg tacgtttgaa tgtaaaacca actcgtatgg aattgaataa cttaaaggaa
     4501 cgtttgacaa cagctgaacg tggacataag ttattaaagg ataaaagaga tgaattgatg
     4561 aggcgattta tttctttgat tcgtgagaat aatcaacttc ggaaagaagt ggaaagttat
     4621 ctaattgata atctaaaatc ctttgcagtt gctaaatcat taaagaattc tcaaatggtg
     4681 gaggaattat tttcaattcc atcgaaagaa attgaattat ttgttgagaa agaaaatatc
     4741 atgagtgtaa cagttcctag aatgcatatg aatattactt ctcaaaatga gaacagtgaa
     4801 tacagctatt tatcttctaa tagtgaaatg gatgatgtat ttgctacaat gaatagttta
     4861 atttataaat tactaagact ggcagaagtt gaaaaaacgt gtcagttaat ggctgatgaa
     4921 atagaaaaaa cacgtagacg tgtaaatggt ttagaatact cgattattcc aaacttgtcg
     4981 gaaactattc attatataga attgaaacta gaggaggcag aaagagccaa tttagttcgt
     5041 attatgaaag tgaagtagat cctttattta gattattaat tagatgaaca aatatcagct
     5101 tggataaggc tttaagcctt tctaagcttt ttttattgac agtatcagga tatctttttc
     5161 aaaattttgg tttgtt
//
LOCUS       DAVLYV010000103         5069 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLYV010000103 DAVLYV010000000
VERSION     DAVLYV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.103"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            70..1329
                     /locus_tag="VYO70_001928"
     CDS             70..1329
                     /locus_tag="VYO70_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1714093.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1405..1540
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1579..2079
                     /locus_tag="VYO70_001929"
     CDS             1579..2079
                     /locus_tag="VYO70_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1714094.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2155..2523
                     /gene="rplL"
                     /locus_tag="VYO70_001930"
     CDS             2155..2523
                     /gene="rplL"
                     /locus_tag="VYO70_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1714095.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            2710..2883
                     /locus_tag="VYO70_001931"
     CDS             2710..2883
                     /locus_tag="VYO70_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714096.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQS"
     gene            2880..3128
                     /locus_tag="VYO70_001932"
                     /pseudo
     CDS             2880..3128
                     /locus_tag="VYO70_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3142..3465
                     /locus_tag="VYO70_001933"
     CDS             3142..3465
                     /locus_tag="VYO70_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714097.1"
                     /translation="MTFLDDYHKKHNYPLFYESYLQNIMEFLESQDIKNGADAFVDDH
                     QNLVFVLYGQGYRAEGKEGILTTQVTVKAYDEDKQPINFANLLDSLIVSEYQMEPNLW
                     EVSHD"
     gene            3458..3838
                     /locus_tag="VYO70_001934"
     CDS             3458..3838
                     /locus_tag="VYO70_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1714098.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFIEMILKNPELTIRLPLAVSNKRVYPNLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3851..4045
                     /locus_tag="VYO70_001935"
     CDS             3851..4045
                     /locus_tag="VYO70_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1714099.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIV"
     gene            4087..4674
                     /locus_tag="VYO70_001936"
     CDS             4087..4674
                     /locus_tag="VYO70_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1714100.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYCASLIS
                     FLWMYAQRKQAHKVNMKSRIKWIGIGLASLLIISLCFSLIHAQESTNQANLIGLQYQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            4876..5013
                     /locus_tag="VYO70_001937"
     CDS             4876..5013
                     /locus_tag="VYO70_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1714101.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
BASE COUNT         1612 a          849 c         1077 g         1531 t
ORIGIN      
        1 acctacggca aagtgaatct gaagtggttt gaagagagta caacttgtct tttaaaaaag
       61 gagtctataa tgaaagtctt tcagcatgta aatatcgtga cttgtgatca aaatttccat
      121 gtttatcttg atggaatctt agcagtcaag gattctcaaa tcgtctatgt cggtcaagat
      181 aagccagcgt ttttagaaca agctgagcag attatagact atcagggagc ttggattatg
      241 cctggtttgg tcaattgtca cacccattct gcaatgacag gtctgagagg gatccgagat
      301 gacagcaatc tccatgaatg gctcaatgac tatatctggc cagcagaatc tgagtttact
      361 cccgacatga ctaccaatgc ggtcaaagaa gccctgacag agatgctcca gtcaggaaca
      421 acaaccttta acgatatgta taatcccaat ggtgtggata tccagcaaat ttatcaggtg
      481 gtgaaaactt ccaagatgcg ttgttatttt tctccgactc tcttttcttc agagacagaa
      541 acaactgctg agactataag cagaactcga tccatcatag acgaaatctt aaaatataaa
      601 aatccaaatt tcaaggttat ggtagcacct cattctccgt atagctgcag tagagacttg
      661 ctggaagcga gtttggaaat ggcaaaagag ctaaatattc cgctccatgt ccatgtggcg
      721 gagaccaagg aggagtcagg aattatcctc aaacgatacg gcaaacgccc ccttgctttt
      781 cttgaagaac tgggttattt agatcatccg tccgtatttg ctcacggggt cgaattaaac
      841 gagagagaaa ttgaacgctt ggcatcttct caagtggcta tcgcccacaa tcctatcagt
      901 aacctcaaac tggcatcagg aattgctcca attatccagc tccaaaaagc gggagtagca
      961 gtcggaattg cgactgactc ggttgcttcc aataacaatc tagatatgtt tgaggaagga
     1021 aggactgcag ctcttcttca gaagatgaaa agtggggatg ccagccagtt tccaatcgaa
     1081 acagctctca aggtactgac aatcgaaggg gctaaggtcc ttggaatgga aaatcagata
     1141 ggaagtctgg aagtcggcaa gcaagcagat tttctggtca ttcaaccaca agggaaaatt
     1201 catctccaac ctcaggaaaa tatgctgtct cacctggttt atgcagttaa atctagtgat
     1261 gtagatgatg tttatatcgc cggagaacag gttgttaagc aaggtcaagt cctgacagta
     1321 gaactttaaa agaaaaatca cgaaaaattt taaaaaaagt ttgcaaaaat cttgcattct
     1381 ttttttgact atgctatact tatatacggt ttaaaaaaac tgcctaagac agtaggggag
     1441 ctcgactcat aaatatccta ccgaggacaa gacgtatcat gtaaaaagaa gcgtattgta
     1501 ctttcgtgtc taggtttggg cgcgtttttc tttttgaaaa attccccaag caaaataatt
     1561 acggaggtga acacactaat gagtgaagca attattgcta aaaaagcgga actagttgac
     1621 gtagtagctg aaaaaatgaa agctgctgca tctatcgtcg ttgtagacgc tcgtggtttg
     1681 acagttgagc aagatacagt tcttcgtcgt gagcttcgtg gaagcgaagt tgagtataaa
     1741 gttattaaaa actcaatctt gcgtcgtgca gctgaaaaag ctggtcttga agatcttgca
     1801 tctgtatttg ttggaccatc tgcagtagca ttttctaatg aagatgttat cgcaccagcg
     1861 aaaatcttga acgacttttc taaaaacgct gaagcacttg aaattaaagg tggtgcaatc
     1921 gaaggcgctg tcgcatctaa agaagagatt cttgcacttg caactcttcc aaaccgcgaa
     1981 ggacttcttt ctatgctcct ttctgtactt caagcgccag tgcgcaacgt tgctcttgca
     2041 gtcaaagcgg ttgcagaaag caaagaagac gcggcttaat cttaagctac acagcgtagc
     2101 ctagctacga aaaaaactat tataaaattt aaaacttatt tggaggaaat aacaatggca
     2161 ttgaacattg aaaacattat tgctgaaatt aaagaagctt caatccttga attgaacgac
     2221 cttgtaaaag ctatcgaaga agaatttggt gtaactgcag ctgctcctgt agctgttgct
     2281 gcagctgatg cagctgatgc tggtgctgct aaagattcat tcgacgttga attgacatct
     2341 gcaggcgaca aaaaagttgg cgttatcaaa gttgtacgtg aaatcactgg tcttggtctt
     2401 aaagaagcta aagaacttgt tgacggtgca ccagcacttg ttaaagaagg cgttgcaact
     2461 gcagaagctg aagaaatcaa agctaaattg gaagaagctg gagcttcagt tactcttaaa
     2521 taatagagta tcgcaatttg attgcggaat ccctatttac cagtctttga gagcgataag
     2581 cgattgatag aaactgataa attgatttgc tttcctgcca aaaattcttt ggcaggattt
     2641 ttgttttaat aaactttcgg gggtagacac tagattgtct accccttttt ttgtgtttcg
     2701 gaggtaatta tgcaaaagga aggacagtca tcacgtcaat tcgtgaagag tcatctcatg
     2761 actatcatgg gagttgatat tgtaaaagca actcagttag ttgatgaaat ggaacaagta
     2821 ggtttaatcc gatttgatga atttggaaat gttggaattt tagtcttgga gggacaatca
     2881 tgaagcgtat taccgctagt caatatcaga catcagagcg ttattataag ctccccaaac
     2941 ttttatttga gagtgaacgg tataagaata tgaaactgga ggttaaggtg gtttactcag
     3001 ttttaaaaga tcggttggag ttgtctttga gcaaaggttg gattgatgag gatggggcta
     3061 tttatttgat ttattccaat tcaaatttga tggcactttt aggctgttca aagtcaaaat
     3121 tactctccat gtgagtttga agtgacattt ttagatgatt accataaaaa acataactac
     3181 ccactatttt acgaatccta tcttcaaaac attatggaat tccttgaaag tcaggatata
     3241 aagaacggag ctgatgcctt tgtagatgat catcaaaatc ttgtttttgt tttatatgga
     3301 caaggctatc gagccgaggg aaaagaggga atacttacaa cccaagtaac tgtaaaagct
     3361 tatgatgaag acaagcaacc gattaacttc gcaaatttat tagattcctt aatcgtctca
     3421 gagtatcaaa tggaaccgaa tctttgggag gtctcccatg attgatctct atctaagtaa
     3481 aaatagccaa agaaatcaac ttcttttaga cttcttccaa aactatggca tcgaggtatc
     3541 ttgtcattca gtttctgaaa tgacaaagga taaattaatt gagatgatga gctattcttc
     3601 agattgtttt gagtttttat ctcccaattt attacgattt aagaaccgtg acaatttaag
     3661 attaacggat ttcattgaaa tgatattaaa aaatcctgaa ctaaccatca gacttcctct
     3721 tgcagtttca aataagagag tttatccaaa tctaaatttg gaggaagcta gagctttatt
     3781 gccaagagat acgaaacaat tgatttacat ggcgcaaaca cattatttat ctaactaaag
     3841 gagaagcaat atggctgaac gattttggga gaacttgtcc attattttgg ctgaaagaaa
     3901 tatcagctgg attgagctaa ccagaaaaat gtttgcggga gagtttcact atccaagtga
     3961 attgaatcgt ttgtatcaaa agattcgtca ctacaagatg gaacaacgaa tgcctcaaag
     4021 tccatgggta gaaaggattg tgtaggtatt agatttagat tatgaagatt tatttcggag
     4081 gtaactatga aaagaataat tccagtttat atattccaac aagtaaatgt cctattggta
     4141 tctctatact tactgaaatt tctttgtatc ggtgagttaa ctatactaca gattctatat
     4201 tgtgcgtcac tcatttcttt tttatggatg tatgcccaac gtaaacaagc gcataaggtc
     4261 aatatgaaat caaggataaa atggattggt attggattag ccagtctact gattataagt
     4321 ctatgtttta gtctaatcca tgcgcaagaa agcacgaatc aagcaaactt aattgggctt
     4381 caatatcaag ttccttggtt ttcattttta ttgttcttaa tcaatgcgag tatggttgaa
     4441 gaatttctgt atagagaaat tttatggaac ttggttagaa aattagatat tcgagttgct
     4501 ttgacaagtg ttttatttgc cttagcacat catccaggaa ccattatagc ttggtgtttg
     4561 tatgtatcgc tggggatgtt tttagggctt gtacgctaca aattggactt atggggcagt
     4621 atggggttac atttggtgtg gaacctacta gtttatagtt tgctgctttt ttaaaatagg
     4681 taacctgatt tttaggtaac ttctaaaact aacttccagt tttgggagag agatggaagt
     4741 tactttgaaa agttacggaa acattatttt ctatgtgaca acattgtata attgcttgca
     4801 ttttactggt ggagagagta taatgagggt ggataaagta cttaatccca aaaatttaaa
     4861 taggaggagc tagctatggc agaagaagta ttaaatcttc aattagtatc tgttcaagtt
     4921 gacgaaacag atgaagttga tggaatgcga tttagtacat tcagtacaaa tcgttgcggt
     4981 aactggagtg cttttagttg ggaaaattgt taattcattt aaattgtaaa ctttagagat
     5041 tctctggagt tatggatgtc gttccaata
//
LOCUS       DAVLYV010000104         4993 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLYV010000104 DAVLYV010000000
VERSION     DAVLYV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4993
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.104"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..490
                     /locus_tag="VYO70_001938"
     CDS             <1..490
                     /locus_tag="VYO70_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714102.1"
                     /translation="KSIKHFTSFSFKLAALSTGIWTATLFLLIFLIAFSNGFSFSLEI
                     KEVDFLGEFYGISIANNASFFIGFFFSYIAYYFFLSLLTISSFSWFKKSNMSLVFLFT
                     FLFVESLFWIYQLNNGIIGLLPIFQYMVNSNPYALIYWLTLLSIIIPLTVFSVHRNWR
                     RV"
     gene            500..1198
                     /locus_tag="VYO70_001939"
     CDS             500..1198
                     /locus_tag="VYO70_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714103.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLANWDIGVLFIVDSLNACVLSLLCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            1195..1842
                     /locus_tag="VYO70_001940"
     CDS             1195..1842
                     /locus_tag="VYO70_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714104.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRELSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(1922..2787)
                     /locus_tag="VYO70_001941"
                     /pseudo
     CDS             complement(1922..2787)
                     /locus_tag="VYO70_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(2799..3556)
                     /locus_tag="VYO70_001942"
                     /pseudo
     CDS             complement(2799..3556)
                     /locus_tag="VYO70_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(3557..3844)
                     /locus_tag="VYO70_001943"
     CDS             complement(3557..3844)
                     /locus_tag="VYO70_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1714105.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            complement(3854..4234)
                     /locus_tag="VYO70_001944"
     CDS             complement(3854..4234)
                     /locus_tag="VYO70_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1714106.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESE"
     gene            complement(4194..4874)
                     /locus_tag="VYO70_001945"
     CDS             complement(4194..4874)
                     /locus_tag="VYO70_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1714107.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
BASE COUNT         1483 a          833 c         1062 g         1615 t
ORIGIN      
        1 gaaatcaatt aagcacttta ccagttttag tttcaaatta gcagctctta gtacggggat
       61 ttggacggcg actttatttt tattgatttt tctaattgca tttagtaatg gttttagctt
      121 ctctttggag ataaaggagg ttgatttttt aggagaattt tatggtataa gtattgcaaa
      181 caatgctagt ttctttatag gatttttttt ctcttatata gcatactatt tctttttatc
      241 cttacttact attagcagtt tttcttggtt taaaaaatca aacatgagct tagtatttct
      301 gtttactttt ttatttgtag aatccttatt ctggatttat cagttgaaca atgggataat
      361 tggattattg ccaatttttc agtatatggt aaattccaat ccgtatgcat tgatttattg
      421 gcttacatta ctatctatca taattccatt gactgtattt tctgttcata gaaactggag
      481 gagagtgtaa aagttggaaa tgggaaagtt aagtagtcac atgtggaggt tgaatcagat
      541 aatctatacc aagtactttt ggggttatgt tcttttttgg atattgattt gtttaggatt
      601 atggtattgg ttagaaggaa atgatagact tgttatagaa attttaaaag ggcctaatct
      661 gagtcaaaac tcttttttag tcttatctat atggttgctt cattggttta ttattcatac
      721 attttttcta gcagttgtat atcgtagaag agcatccgat ttctttatgg aagtgattcg
      781 attttcttct attaagctct ggattaggta tcagatttgg acctgttttc tttatggact
      841 cattttaatc atggtaaaag ttctagtgat tcaatttatg ttacagttag caaactggga
      901 tataggagtt ttgtttatag ttgattcttt gaatgcttgt gtgctatcct tgctttgctt
      961 tatgttatac gcactaggag cgaatgtaca aatgaacttt gcttgcgtta gtttcttttt
     1021 actcatgatt gtgtttggtg gtctatttgt aggaaatcga actaattatc tattttatat
     1081 tttgaatcga gggaatggag atataggacg agatttattt cttcagttgc tatttttagt
     1141 ctttcttttt aaaagcattt tctatttcac tcgtcaaaag aggaggttta tagaatgaat
     1201 gaaataatta cattaaaaaa tattgagttg aaattaaaaa aaacatgtgt ttttcaaaac
     1261 cttaatttta gttgtaaaca gggggaaatt ataggaatta ctggtgcgaa tggctcaggt
     1321 aaaagtgtat tgtttaaatt aatagctggt ttatatagtc cgtcttatgg agaagtgtta
     1381 atcaatgggg aaaatattgt tcctgagaga aaaattccag ctaatttggg agctttgatt
     1441 gaagaacctg gttttataaa ttattatagt ggctttaaga atttacaata tttggcaagc
     1501 atacgaggag tagttggtaa tcaggaaatc aatgatacac tgaaaatagt tggtctatat
     1561 gagcaaaaag accagaaagt taaaacttat tcgctaggta tgaggaaaaa gctagggatt
     1621 gctcaagcaa ttatggagaa tccctctatt cttttactag atgaacctat gaatgccttg
     1681 gataaatcaa gtgtagaaaa tatgagaaca ttgtttagag agctctctag tgaaaaaggg
     1741 acaacaattt tgattgctag tcatagtgaa gaggatattc gtatcttatg tgataaagta
     1801 tatgcaatag aagataaagt atgtacactg tgttcagatt gaacatctta gatagactca
     1861 ggatgttata ggtaaaatct taaaaaggga ttggagctta aaatccagtc ccttttaaga
     1921 gttaatggcc accttcaaca accaggtcat ctgctttagc agcgtcaccg taggttgggt
     1981 gaagtgtttt ttcataggcc ttgtggaaga agttttcctt gcctaatttt tcaatatctt
     2041 tattgatgaa gtctagcaat tcttggttgt ctttttgaac tgctgccgca atggtatcgg
     2101 gatcaccgag ggaagtaatt cctacttcaa atcctttatt ttcaagcgcc caagctagaa
     2161 cttccgtatt gtcagttgaa aaggcatctc cacgtccgtc aagaagagct tggtaagagt
     2221 cactgtattg gtcgtatttt tggagtttga tttctggatg attcttttca aaataagtct
     2281 cagcagtcgt tccttttgtg acaattaagg ttttaccttc aagttgtttg acgtctgtaa
     2341 tgagaccagt cttaggtgat acgacaccca gagaaacttt catatatgga agggcaaaat
     2401 caacttgttt cttacgttcg tcagttactg taaagttagc aagagtaata tctaccttgt
     2461 ttgaaatcaa gtattccgca cggttggcgg catcgactga aatgtattta accttgacac
     2521 caaggtcttg agctagttgg ttccctagtt caatatcgta gcgtagcctt ggtaagaacc
     2581 attattgtca acgtagccaa acggtttttt atctccaaac acggcgattc gcagttcacc
     2641 gctttttttg atttcatcga tagtgcgagc tttggcagtt gtttttccag aggatgaacc
     2701 agcgtttcca cctgagctac aagcagtgat aaagataagg gcaaaggcaa gtgctaaaac
     2761 agttaagagt ggtttgaata gtttcataag aatctccttt atagatatga gccgaattgg
     2821 ctaaagtcaa agacgtttaa aaattcctgg gctcgtttgg tttgcagatt ggtaaagaag
     2881 gcttgagctg ttccttcttc agcgattttc ccttggtcga ggaagataat ccggtcagta
     2941 atggcttggg caaactgcat ttcgtgggtt actaaaatca tggtacggcc ttcttgggcc
     3001 aaatcattga taagttccag cacctcacgc accatttctg gatccagcga agcagtcacc
     3061 tcgtcaaaaa ggatgatttc tggatgcatt aggagggcac ggacaattgc aacacgttgc
     3121 ttctgtccac cagataattg acgggcaaag ctatgttgtt tatccagcaa accgacacgc
     3181 tctagtaatt gcaaagcttc ttccgttact tctttcttgt cccttccttg agctttgata
     3241 gggcctagga tgaggttttg taagacatcc agatggggaa agagttcata actttgaaag
     3301 accatgccaa tcttttggcg aactaggtga aaatcttttt tattttcaac gatagactga
     3361 ccatccagaa gaatatctcc accttgaata ctttctaagc cgttgaggca acgaaggagg
     3421 gtacttttcc cacaaccaga tggccctagg atgacaacaa cttccctttt ttgatttcta
     3481 gagaaagtcc ttggaggatg ggattgtctc cgaaggattt ttttagttcc ttgatttcta
     3541 agatagtttc agacatttag tttctccaat gtttttctaa gtgagtggat agtttggaaa
     3601 tagggtagca aactgcgaaa tataagacta gaatggttcc ataaatccaa aatgaagcag
     3661 ttgggatggt caggcgattg ctatcgatga tttgttgtcc aactttggtc acttccacaa
     3721 ccccaatcaa aacaactaat gaagtggttt taatcatccg agtgacaaga ttgatagcct
     3781 gcggtagcag tcttcttaag acttgtggga tgatgatgtg gtagtaaagt tgaacattag
     3841 tcaagccgag tgcctattca ctttcaaact gatgtttagg gagagaagtg atagctccac
     3901 gtaccaagtc tcccatttca gctgttcccc agagggtaaa aatgataata gctgaagtct
     3961 cacctgagat attgatatta aagtttcgag ccaagccaaa gtaaacgatg aagagtagca
     4021 ccagctgggg catgatacgg ataaattcca gatacaatcg tgttaaaaat cgtatgattc
     4081 tagaatggga ggtcatgatg attcccatga ctgttccgaa catcatggat aagaggacag
     4141 acaggatgga tatcccaatc gtaacgccca acccctgtaa gattctcagg agattatttc
     4201 cttgaaagag tacttggatt cccgaatcct gcatggcggg tcctcctttc tatccagcta
     4261 aagaccagtg agatgggtag cagcatgatc agataagcaa ctaccaacat agctagcgca
     4321 atgtctgtct catagtagag accaatcaaa tccttggcga cgtacatgag gtcggccaaa
     4381 gccactgctg agaaaacaga ggtttccttg ataaggaaaa tgacattggc actaaaggag
     4441 gggagtgcca ccgctgttgc ttgcggaaga accacatagc gaaagacctg tagaggtgtc
     4501 agaccaatag cgaggccaat ctcctgctgg gtttgactga tggcttccag cccacttcgg
     4561 aaagattctg ccatatagga gcctcctaaa aagacaagcc caagcgttgc acagacttct
     4621 gaagatagga caatcccgat tcggggaaga ccgaagtaga gaaagaagag ttgaatcaaa
     4681 aggggcgtat tacgtgacaa ttcaatgtag gctgtcgcta cttgcgccaa aacagggatt
     4741 cgataatgtc ggatgatact aacgattaaa ccgagcagaa aggatcccaa aattccccaa
     4801 actgcaatat gcaaggtcag aaagaatgcc ttttgatata gtggtagata ttgttcaaca
     4861 atggaccaat ccaaaaatag aacctcccat ctagaaataa tacagttatt gtagcactta
     4921 aaatcttctt tggataatat ctatttttta ttgccgttat aaggattttt atcatagaca
     4981 taaaatttct gaa
//
LOCUS       DAVLYV010000105         4884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLYV010000105 DAVLYV010000000
VERSION     DAVLYV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.105"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            53..649
                     /locus_tag="VYO70_001946"
                     /pseudo
     CDS             53..649
                     /locus_tag="VYO70_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            725..1174
                     /locus_tag="VYO70_001947"
     CDS             725..1174
                     /locus_tag="VYO70_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1714108.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVGLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            1187..1846
                     /locus_tag="VYO70_001948"
     CDS             1187..1846
                     /locus_tag="VYO70_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714109.1"
                     /translation="MSEIKIINAKKIYHDVPVIENLNITIPKGSLFTLLGPSGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMNEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     K"
     gene            1973..2278
                     /locus_tag="VYO70_001949"
                     /pseudo
     CDS             1973..2278
                     /locus_tag="VYO70_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            2268..3920
                     /locus_tag="VYO70_001950"
                     /pseudo
     CDS             2268..3920
                     /locus_tag="VYO70_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4023..4715)
                     /locus_tag="VYO70_001951"
     CDS             complement(4023..4715)
                     /locus_tag="VYO70_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1714110.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1525 a         1047 c          842 g         1470 t
ORIGIN      
        1 acgcttatat tgttttaaaa taaacttaaa taacacaacg gaggtatcca tcatgaaaaa
       61 gaaatggatg tattatgctg cttgttcttc taatgaatct gccgatgaca gttcatctga
      121 taaaggagac ggtggttcgc tagtcgttta ttcaccaaac tcagaaggct taattggagc
      181 aactattcct gcctttgaag aaaaatatgg tatcaaaata gaactgattc aagctggtac
      241 tggagaactt ttcaaaaaac tagagtcaga aaaagaagtt cctgtagctg atgttatctt
      301 tggtggttct tatacacaat atgctaccca cggagaactc tttgaaaact atatttcaaa
      361 agaaaatgat aatgttatca aagaatatca aaacacaact ggcttctcta ctccttatac
      421 actagatggt agtgttttaa tcgtccaccc tgatttaact aaaggcatga acatcgaagg
      481 atatagcgac cttctcaaac ctgaactaaa aggaaaaatc gcaactgctg acccagcaaa
      541 ctcttctagc gcctttgctc aattaacaaa tatgctacaa gctcaaggtg gttacaaaga
      601 tgataaggct tggtcttatg taaaagatct tttcacactt attgatggta tagtaaaatg
      661 aaataagaac aagacaaatt aatcaggaca gtcaaatcga tttctaacaa tgttttagaa
      721 gtagatgtgt actattctag tttcaatata ctataaaatc ggtttaagtt catctggtgt
      781 ctataaagca gtcgctgatg gagaaatgac tgttggtctc tcttatgaag atccagcagt
      841 taaactctta aatgacggag ctaacattaa ggtagtctat ccaaaagaag gaaccgtctt
      901 cctacctgct agtgctgcta tcgttaaaaa atctaaaaat atggaaaatg ccaagaaatt
      961 tatcgatttt attatctctc aagaagtaca agatacactt ggtacaacca ctactaaccg
     1021 tcctgttcgt aaaaatgcta aaacaagcga aaacatgaaa ccaattgaca aaatcaaaac
     1081 actcactgaa gattatgatt atgtcatcaa gaataaatca gatatcgtta agaaatacaa
     1141 cgaagtcttt acagatatcc aatctaaaca gtaaaagagg ttcactatga gtgagatcaa
     1201 aattattaac gccaaaaaaa tctaccacga tgtccctgtt attgagaatt tgaacattac
     1261 aattccaaaa ggaagtctct ttacccttct tggaccttca ggatgtggga aaacgaccct
     1321 tcttcgtatg attgcaggtt tcaacagtat cgaaggtgga gaattttact tcgatgatac
     1381 aaaaatcaat aatatggaac ccagcaaacg caatatcggg atggttttcc aaaactacgc
     1441 tattttccca catttgactg tccgagacaa cgttgctttt ggtcttatgc aaaagaaggt
     1501 tccaaaagaa gaattgattc aacagaccaa caagtatctt gaactcatgc aaattgctca
     1561 atatgcggat cgaaagcccg ataaactcag tggtggacaa caacaacgtg tcaccttggc
     1621 atgcgcctta gcggttaatc caagtgttct cctcatgaac gagccactta gtaatctgga
     1681 ggccaaactt cgcttggata tgcgtcaagc catccgagaa atccaacacg aagtgggaat
     1741 tacaactgtt tatgtaaccc acgaccaaga agaagccatg gctatttcag accaaattgc
     1801 tgttatgaaa gatggggtga tccaacaaat cggccgacca aaataactct atcataaacc
     1861 agctaatgag tttgtggcaa cctttatcgg acgcacaaat attatccctg ccaatcttga
     1921 aaaacggagc gacggcgctt atatcgtctt ttcagatggc tatgcccttc gaatgccagc
     1981 tcttgatcag gttgaggagc aagctattca tgtaagcatt cgtcccgaag agtttatcaa
     2041 agatgaatct ggagatatcg aaggaactat tagcgatagc gtctatcttg gactaaatac
     2101 ggagtatttc attgagacag gttttgcctc aaaaatttaa gttagtgaag aatcaacttt
     2161 tgaagaagaa ctacaaaaag gcaatcgtat tcgtctacga atcaatacgc aaaaattaaa
     2221 catcttttct gtagatggtt cccaaaacct gataaaagga gtcaaccatg gaatgtaaaa
     2281 aactaaatat ttggacagcc tcctctttct tcatctttct tacctatctt gtctttctcg
     2341 tttatcctat cgttaccgtg ctcaagcaag cacttataca tgaaggacaa ttctcactat
     2401 ctaattttgt cactttcttt agtaaagcct actactctga gacactagtc aacagtttca
     2461 gggtttccat taccgctact gtcacttcct tagttgtagg aaccctatta gcttatctct
     2521 tctctatgta tgacttcaag gggaagaaat ttctacaaat attgattatc attgcttcca
     2581 tgtcagctcc tttcgtagga gcctactcct gggttctctt gctgggacga aatgggggca
     2641 tcactaaatt tttgacaaat gcccttcatc ttccagctat cgatatttat ggattcaaag
     2701 gaattgtact tgtctttaca ctgcaactat tcccactggt atttctatat gttgctggaa
     2761 caatgaacag tattgacaat tctctacttg aagcagctga aagcatgggg tccttcggat
     2821 ttaaacctat cgtaacggtt gttttacctc ttctagttcc aaccttacta gcagctcctt
     2881 gcttgtattt atgagagcat tctcagactt tggaacgcct atgttgattg gcgaaggata
     2941 tcggactttc cctgtcctga tttataccca atttattagc gaggttggag gaaattctgc
     3001 ttttgcaatt atggcgatta tcattgcctt ggcaattttc cttatccaaa aacacattgc
     3061 aaaccgctac agtttcagca tgaatccgct ccatccaatt gagcctaaaa aaactacaaa
     3121 aggaaaaatg gctgccattt atgcaacagt ctacggaatt atctttatct ctgttttacc
     3181 tcaaatctac ttaatttata cctctttcct aaaaacatca ggtatggtat ttgttaaagg
     3241 ttattctcca aacagttaca aggtagcttt caatcgtatg ggatctgcta ttttcaatac
     3301 cattcgtatc cctttgattg ccttagttct agttgttcta tttgcgacat ttatctccta
     3361 cctagccgtt agaaaacgga atttgtttac aaacttaatt gacagcctca gtatggtacc
     3421 ttatattgta tcaggaaccg ttctagggat tgccttcatt tcttccttca atactggtct
     3481 atttggaagt ggatttctta tgattacagg gactgctttc atcttgatta tgtctctatc
     3541 tgtcagaaga ttaccttata ctattcgctc atctgttgct agcttacaac aaatagcacc
     3601 aagtattgaa gaagctgctg aaagcttagg aagtagtcgt ctcaatacct ttgctaagat
     3661 tacaactcca atgatgctat cgggtattat ttctggagcc atcctatctt gggtcacact
     3721 gatttcagaa ctctctactt ctatcctcta tcctcctcta caatgtcaaa acaagaacaa
     3781 tgactgtagc tatttataca gaggttctca gaggaaatta cggtgtagcc gcagccttgt
     3841 caactatcct gactgttcta acagtaggtt ccttgctctt gtttatgaaa atctctaaaa
     3901 gcaatagcat tacactttag ttttccacat caaaaacagc cgaaaggagt accctcggct
     3961 gttttttctt atcttgatat tcttatcaaa tccctagctc atggcaagca agtctagact
     4021 gattaaatag agccttcttc catcttctca cgtcctagct ctcggtaact acggtcaccg
     4081 acaacttcaa ccctaaactg accgtcctca tattcaagaa tcgtcacgct accattatct
     4141 agaccatgcg gatgcatgcc attaatcaga taaacaatgg ttccaatagt cattccatgg
     4201 ctgacaacaa gggcgttacc tccaccttga tcttccattt cttttgcaat catttcaaag
     4261 ccttccttaa ttcggccact gagtttttcc cagccttcag cccaaccagc tgtatcgacc
     4321 tctaccaagc cctcagccag ttcagcataa gacaattggt gaacgtggtc cacattaaag
     4381 atacgaggaa taatgcccat gaaaagatcg ccatcatagg ctccatcaaa actaccgaaa
     4441 caccattctc tgatacgctt gtccatgcga taagggattt tcccctgcaa gccaagctct
     4501 tcaaggataa ttcccatggt ctgaatggta cgaccagaat cactcgaata agcgcgctca
     4561 aactgtagat cagattctcg caaaccaatt cctaactctt gaatccctcg ttcaccttca
     4621 gcagttaagg gagtatcgct ccaaccttgc gcgcgaccaa tcgtgttaaa catggtcttg
     4681 ccatgacgta ccaaatacaa tcgtactttt accattttcc gtcctccgtt tgcttctatt
     4741 atatcatgaa tgataaaata aaagccaccc atacaggcga cttttgaagg agattattat
     4801 gaaaaagttt aggagttcat taaataaaga tattagatga aaatcaaatt caaactaatt
     4861 cagtgttatc tgtttcaaat aata
//
LOCUS       DAVLYV010000106         4839 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLYV010000106 DAVLYV010000000
VERSION     DAVLYV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.106"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(356..1084)
                     /locus_tag="VYO70_001952"
     CDS             complement(356..1084)
                     /locus_tag="VYO70_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1714111.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1242..2651
                     /locus_tag="VYO70_001953"
     CDS             1242..2651
                     /locus_tag="VYO70_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1714112.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVEGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKKEYGNIPWFISENGMGVENEARFIGENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKRS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2669..3964
                     /locus_tag="VYO70_001954"
     CDS             2669..3964
                     /locus_tag="VYO70_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1714113.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3969..4277
                     /locus_tag="VYO70_001955"
     CDS             3969..4277
                     /locus_tag="VYO70_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1714114.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALEWIGEIR
                     "
     gene            4274..4582
                     /locus_tag="VYO70_001956"
     CDS             4274..4582
                     /locus_tag="VYO70_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1714115.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1630 a          820 c          888 g         1501 t
ORIGIN      
        1 agattgaaat tcatttctag caatttgtta gaagtcacag tctactattc taaccacagt
       61 atactatatg tgttacaaga aaagactcaa aaacttctcg tcagtgtaag aatgtttgaa
      121 acactattta aaactaaata actataagaa gtagatcagg tgaacaattt cagtacctac
      181 tctataagct atacgaagat atttttcaac atgatggata caacgatcac tcttttaaag
      241 ccatcttatt tagaaaagag aaaccattta agcatagatg ggaccttctt gccctgtttt
      301 aaatttttct taaactagag tctgtaaaag attggcttca ttttcacatt ttctcctagg
      361 aggaatgtcg taatgcaaaa gaattaaatt caaataaatc atagcgatga cgactaatcg
      421 agtactcaaa cggagttccg ttatccaaat aagccacttg ttctacttca aataccggtt
      481 ccgttggaag gagctgcaag taatgttgct cattttctga actagaagaa gctcgtaaga
      541 tttttgtagc actggcaata tgcaatccta actttccttc aatgtaattg taaatcgatt
      601 tttgtaaaat atcttcagta atacctggaa taacatcggt actcatataa gtttgttcca
      661 gaacataagg tttaccgtca ataatacgca ggcggtaaat attataaact ggactctgca
      721 aagcgacctg tagtttttct gctaaaaatt catttgcaaa ttctaatttg aagtgaataa
      781 tcctcgagtc aattgtagaa tgagcatctt cagatatttt tgtcagtccc cggatatctc
      841 tttctggaac gattaatttt ctttttgagc tgccacgaga gagaacaaag gttccctgcc
      901 cacgttttct aaaaattaag ccctcagaaa ctaacaagtc taaagctttt ttgatcgtca
      961 ttcgactaca ggcaaactgt ttagctaatg tcatttcatc aggcaattgt tcgttcatgg
     1021 tatatttatt acaaagaatc ttttcacgaa tctcattgta aataaataaa tattttgtac
     1081 tcatttaatc tggttctctc tataatattc taatagtagt ataacaaagt tcaagaaatg
     1141 aggcaaacac aagatatgta tcaacaattt ttaaaaatgt ctagacaaat tttaaaatta
     1201 tgctatactt tatgtatagc ataatagaaa ggaggttgag catgaaaaaa aagttagtat
     1261 ttcctaatct gttttggtgg ggagctgctt ctagcggacc tcagacagaa ggtcaatatg
     1321 gaaaagtaca tgaaaatgtg atggactact ggttcaaaac gcatccagaa gattttttcg
     1381 ataatgtcgg acctcttgta gccagtaact tttttcatac ttacaccgaa gatttccact
     1441 tgatgaagga aattggagtt aattctttcc gcacttccat ccaatggagt cgactcatca
     1501 agaatttaga gacaggtgag cctgatccaa aaggtattgc tttctacaat gccatcattg
     1561 aagaagctaa aaagaaccag atggatcttg tgatgaattt acatcatttt gatttaccag
     1621 tggaacttct tcaaaaatac ggtggttggg aaagcaaaca tgtagtggag ttattcgtga
     1681 agtttgccaa gactgctttc acatgctttg gagataaggt tcattactgg acaactttca
     1741 atgagccaat ggtcattcca gaagcaggat acttatatgc tttccattat ccaaatctaa
     1801 aaggaaaggg aaaagaggcc gtacaagtca tctataatct aaaccttgct agtgcaaaag
     1861 tgattcaact atatcgctca ttaggacttg atggaaagat tgggattatt ttaaacttga
     1921 cacctgctta tccaagaagt aattctccag aagacttaga agcaagtcga tttacagatg
     1981 acttctttaa caaagtcttc ttgaatccag ctgttgaagg aactttccca gaaagattgg
     2041 taaaacagct agagagagat ggcgtgttat ggagtcatac cgaaaaagag cttcaactga
     2101 tgaaatcaaa tacggttgat tttcttggag taaactacta ccatccaaaa cgtgttcaag
     2161 cacaagcaaa tcctgaggaa tatcagacgc cctggatgcc agaccaatac ttcaaagagt
     2221 atgaatggcc ggagcgtcgc atgaatccat atcgtggttg ggaaattttt ccgaaagcca
     2281 tttatgatat tgctatgatt gtgaagaaag aatatggtaa tattccatgg tttatcagtg
     2341 aaaacggaat gggtgttgaa aacgaagcac ggtttatcgg tgaaaatgga gttatcgatg
     2401 acgtgtatcg tattgaattt tatgaagaac atttaagatg gctacataaa gccattgaag
     2461 agggaagtca ctgttttgga taccacgctt ggaccgcatt tgattgctgg tcttggaata
     2521 atgcatataa gaatcgttac ggatttatct ccgttgattt agaaacgcaa aagagaacca
     2581 tcaagagatc aggaagatgg tatcgcaaag taagtgacaa taacggtttt gaagtagaaa
     2641 ttgaggagta aagagaggag atattcagat gaaaaactca aaatttatag atcaatttgc
     2701 cacctttgct ggtaaactag ggaaccaaat tcatttaaaa accctaagag atgcattcgt
     2761 aacagtaatg ccattatata ttttggcagg tttaatcgtt cttttgaaca acacggtatt
     2821 taagtggatt ttccaagggg atacattaac aagattccaa tattggggaa taacaattgc
     2881 aaacggtact ttaagtattt caggtatgat tattgctgta atggttggtt atttcttagc
     2941 taaaaacaga gatttcgaaa acccgttagc agcatcaatg ctatcattag tttctttaat
     3001 tgtgatgatg ccaaatacag tttctgtagt tcctgacgga gcaaaagatg cggtaaacat
     3061 ttcaggtgtt ctttcattca acaacacagg tacaggcgca atgttcgccg gggttatcgt
     3121 agcgattatt gcaacagaat tattcattga attatcaaac gttaaagctt tacaaatgaa
     3181 ccttggtgaa aatattccac cagctgttag tagatcattt agcgtattac ttccagtcat
     3241 gaccgtcatc tccttatttg gggttgtttc agcattatta ttcaatataa ctggaatgaa
     3301 cttaatctca atcattacaa tctttattca agaaccaatt cgtcatattg gtacaagctt
     3361 aatcggggtc attattattt actctttagg aaatatgtta tggctatttg gtattcacca
     3421 agcagttatt tacagtgcca tcctagaacc attactatta attaacatta ctgaaaacat
     3481 cactgcagca aataatggac aagccattcc acacatcatc aacttatcac aaatacaaac
     3541 attcgcttta atgggtggta gtggatctac attatgttta ttaatagcaa cattcttagt
     3601 aagtcgcaat gctgtttcta aaaacgtggc taaattatct tttggacctg gtatcttcaa
     3661 tatcaatgaa ccagtattat tcggttaccc aatcgtttat aacatttcat tagctattcc
     3721 atttatcaca gttccagtac ttggtatttt aatcagctac ttagcaacag ttacaggatt
     3781 catgagtcct gcatttatac aagttccttg gactacacca gtattcttaa atgcatggtt
     3841 agcaacagca ggggacgtga gagcagttct agttcaattc atcatctttg cacttggagt
     3901 tcttctatac attccattta tcaaagttaa tgacaaagtt gttgaacaag aaatggaagg
     3961 ttaatattat gaaacaaatt ttattagtat gtaatgcggg aatgtcgaca agtatgcttg
     4021 ttaaaaaaat gcaacaaagt gcgactgaac gtgggattga aatttctatc caagcaaaat
     4081 caatgacaga agctaaaaaa aatatccatg aagcagatgt cattctaatt gggccacaaa
     4141 ttcgttatga gttacttgct gtgaaagaaa ttgcaggaaa tatccctgta gataccattg
     4201 atatgcggga ttacggaatg atgaatggag caaaagttct ggaacaagca cttgaatgga
     4261 taggagaaat aagatgacag aagaaatgga atatatctgt tttcagctca ttgccaatag
     4321 tggagcagcc aaatcatcct ttattgaagc catccaacta gctaaggcag gaaatttaaa
     4381 agaagcgaaa attaaagttg aagaagcgga agactcctta gtagaagcgc acaagattca
     4441 ctccaatctc atacaaaaag aagctactgg agaaaaaata ggattctcct tgctatttat
     4501 gcatgcagaa gaccaaatgg cttcaacaga gattattcag ttgctatcta aagaattcat
     4561 cgatctctat caaaataaat aggaacactg tcgttcccat ctgtctttat taatgacgac
     4621 tacattgtag gaacctccaa aagttggaca aaatgattga aaggattgga tcctgcatta
     4681 cacaagatcc aatccttttc ttttactcta gtttttataa tatgttgaat ctagaatagt
     4741 ccactgcagt ttctaaaaaa ttgctggaaa tagatgttaa gctccaattt atttgtttat
     4801 atcttatttc agtttactat actagcttat tattctagt
//
LOCUS       DAVLYV010000107         4814 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLYV010000107 DAVLYV010000000
VERSION     DAVLYV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.107"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..925
                     /locus_tag="VYO70_001957"
     CDS             <1..925
                     /locus_tag="VYO70_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEV1714116.1"
                     /translation="DYDEKIGSFNTYEIEQTVSKILRVKPNGKIILKSTVPFGFSNKL
                     KRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVVGDETVEGRKIAELFLSISTHSTANI
                     KNVMLVSPTEAEAIKLFSNTFLALRVAFFNELDSFAERRSLNAEVVIKGVCLDPRIGN
                     FYNNLSFGFGGYCLPKDTKQLKKEFIEINAPVIEAIDISNTNRKQFIVKQILERKPKI
                     VGIYKLGMKYNSDNYKESAILSIINELLIVGIKILVYEPNLNVSIDNVIFEKNFELFT
                     KQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            1321..2184
                     /locus_tag="VYO70_001958"
     CDS             1321..2184
                     /locus_tag="VYO70_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1714117.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACDGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQEQMAETYRRLNAIVIKDAIHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TLPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGSNQKEIEHFIQSLQVLENPQLIALFE
                     LRLQQYKKLID"
     gene            2470..2670
                     /locus_tag="VYO70_001959"
     CDS             2470..2670
                     /locus_tag="VYO70_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HEV1714118.1"
                     /translation="MLNLQFAETMELTEAELEIVYGGEFGNNAVIPAGAWGGFGTPWS
                     ITNFWKKNFNDRPDFDSDRRRY"
     gene            2737..3402
                     /locus_tag="VYO70_001960"
     CDS             2737..3402
                     /locus_tag="VYO70_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1714119.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            3474..4700
                     /locus_tag="VYO70_001961"
     CDS             3474..4700
                     /locus_tag="VYO70_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1714120.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICMINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSISAITVVLPSLFTISLVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
BASE COUNT         1448 a          798 c          914 g         1654 t
ORIGIN      
        1 agattatgat gagaaaattg gtagttttaa tacatatgaa atcgaacaaa cggtatcgaa
       61 gattctgagg gtaaaaccta atggaaagat tattttaaag tcaacagttc cgttcggctt
      121 ttcaaacaaa ttaaaaaggc tgtttgatac aaaaaatatc atttttgtcc ctgaattttt
      181 gagagaaggt tgttctatat atgataattt atatccaagt cgcatagttg ttggagatga
      241 gacagttgaa ggaagaaaaa ttgcagagtt gttcctttcg attagtactc atagtactgc
      301 caatattaaa aatgttatgt tagtttctcc tactgaagca gaagcaatta agcttttttc
      361 taacacattc ttagctctcc gtgttgcttt ttttaatgaa ctagattctt ttgctgagag
      421 gagaagttta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa gaattggaaa
      481 tttttataat aatctttctt ttggatttgg aggatattgt cttcccaaag atactaaaca
      541 attaaaaaaa gaatttatag aaataaatgc cccagtgata gaagcgattg atatttcaaa
      601 cacaaataga aaacagttca tagttaaaca aatattggaa cgaaagccaa agatagtagg
      661 aatatataaa ttggggatga aatataattc agataattac aaagagtcag ctattttaag
      721 tataattaat gaactattga ttgtcggtat aaaaatttta gtatatgaac caaatttaaa
      781 cgttagtata gataacgtaa tttttgaaaa gaattttgaa ctatttacaa aacaaagtga
      841 tctaattgtt gcaaatagat gggatagggg ccttgaagct tataaagata aagtttacac
      901 aagggggatc tggataagag attaactatt gatattaaaa aggcaataat ttataaaatt
      961 tgtacaaaaa aatgttaaat tttcacattt tcatagtata ctgtttaata gaaagtaggt
     1021 tctttgtaac cgctccatta taaaatcctt tcactcttcc ggtatactat taggaaaaac
     1081 ataactggag gatattttga gctcacacct catcgttcca gtggctgttc cccaagctcg
     1141 tgtactgccc aaaaaagtta gacattctat ttagaacgag gattgagttc tgtattgtac
     1201 aaggctcggt ccttttagag tcagcttaag gctggctttt ttattttttc taactatcag
     1261 aatattttga caaatgaaca caaataatga tataatatgc aaaagctagg aggtggtagg
     1321 atgattgaaa aaatggaatt gggggaattt tacaaggaat tacgtttggc gagaaagctc
     1381 aagcagtcag atgtggcttg tgacgggcta acagcttctc agttgtccaa gtttgagcta
     1441 gggcagtcca tgttgtctgc tgataagctc atattagcca tccaagggat caatgtgacc
     1501 tttgatgagt ttgggcacaa gctcaacaac tatcaagaat ctccacatat gcagattggt
     1561 agaaaggttg tggatcgctt tgctcatcaa gatatagctg gcttagagca actgttggag
     1621 gaagtcgagc aagaacagat ggcagagact tatcgtcgtt tgaatgctat tgtaatcaaa
     1681 gatgccatcc actcactaga taaaagctac ccactagcag aggaggatag cgagtttttg
     1741 accacctatc tctatgctat cgagtcttgg acctggtttg aactctatct cttttgcaat
     1801 actctgccct tcttgagcaa tcaagatctg atctttttat caacctcctt actcgaaaaa
     1861 tccaaagaat ttaaagagtt agtacacaat cgattgtata tgaagcaagg actcttaaat
     1921 atcatctcag agctcatgga gcgcaaactt ttttcttata tcccaatctt tgaagccgag
     1981 ctggagagta tgctccgtcc atacgatgtt tttgagaaag tgttgtggca gtttttaaag
     2041 aagatgagca ttttccttca aactaaggga agcaatcaaa aagagattga acactttatc
     2101 caatctctgc aagtattaga aaatccacaa ttaatagccc tttttgaatt gcgtttgcag
     2161 caatacaaaa agcttatcga ttagttagaa atggcaaaat aataataatc tcagcaacga
     2221 ttttcaataa tgttaaaatt tgtttcttca tatttctcca ctcctcatta gtagtaactt
     2281 tattatacca gagaagtaat caaattcacc aaaatcgcaa atctgaaatt tctataagaa
     2341 aaaatatcaa atatgcgatt ttctaaaata agccaatttt cgtgttatac tgtacttgta
     2401 aagcacttga agcaaatcct aggtcacaga agtggtttac aaatgaagat aattgaagga
     2461 gtgtaaaaga tgttaaattt acaatttgca gaaacaatgg aattgacaga agctgagttg
     2521 gagatagttt atggagggga atttgggaat aatgctgtta tcccagccgg tgcttgggga
     2581 gggttcggga caccttggtc aatcactaat ttctggaaga aaaattttaa cgatagacct
     2641 gactttgatt ctgaccgccg tcgttattaa tatccaaagc ccttgccttt tcttgggtag
     2701 gggctgtctt tatttcatag tataggagga aatagaatga aaaagataat ctcacaccgc
     2761 tactttatta tagtttttct actagttatt gctgaccaga agttcagtgt tctagtttta
     2821 cgcagcgacc ttgttactgg gctatctgac tttgcctatt atctgtcgga tatgatgttg
     2881 aattttcttg tggttttatt tgctcttatt gctatgattt ggtcggggaa atggcagaaa
     2941 atcaatagta gaaagtttaa aggatcctat cttttctatt ccttcctagc tctgcttgct
     3001 tttgttgttt ggaatttcgt tacctttttt cttttcccac ctactcgaaa tgaaatttct
     3061 tatcaacatg ctgctccgac ttttacaggg gctacagcat ttttgatgta ttttttctat
     3121 cctgtgattg caggtcccat ttttgaagag ataatccatc ggggattggt gatgacagct
     3181 ctggaaaaag gaaagaaatg ggggctagat gtacttggct ctgctatttt atttggggtc
     3241 tcgcacatta gtaatcacgg ttgggtcttg acagactttg tcttctatat gggtggaggt
     3301 cttatatttg cagtcttatt tagaatgaca aagtcaattt attggcctat tggactgcat
     3361 atagtctaca atggtattgg tcagcttttg atgttactgt aattttggtt gttagtagta
     3421 tcgcggtcta ttcttgggga aagattccca tttgaactta agaaggagtt gtaatgaaag
     3481 tatttcttca aaatagagat tttaggcaat taactatcaa ccagtggatt tcaacgcttg
     3541 gggatacgat tttttatctg gccttcctga attatgtggc agatgcgtct tttgcccctt
     3601 tggcgatttt acttatcacg atttcagaaa cccttcccca agttctacaa atctttctgg
     3661 gagttttggc ggattttcaa catcatcgtg tcttgaaata cacagtcatt agttttacaa
     3721 aatttttgct ttactctata gtgtccctat cgctttcagg gcagtctttt tccttgttgc
     3781 tagtaacctt tatttgtatg attaacctct tgtctgacac attgagttat ttttcaggcg
     3841 ccatgctcac tccgattttc attagaatta ttgggcaaga tcatctggca gaagctattg
     3901 gatttaaaca gtcaactgtt agtttagtga aaacaatcag taatatccta ggaggagtct
     3961 tactaggtat tctatccatc cagtttattt ccttactgaa cgctcttacc tttttaatcg
     4021 catttttagg tatcctcttc ataaaaactg acctcttgaa agtagaaaaa acgattagct
     4081 atcaagaagg actctctgta aaatcctttt gccagcattt gctccaatca tcgaaattga
     4141 tatggaatat gaataaggtg ctcttggttt tgtttattat ctctactagt caagcagtga
     4201 taaatgttac agttcctgtc tctactctat ttttgaggaa tcaacccttt ttgaatttac
     4261 aaacaggtca atctcttgcc ttgctatcca cttttgaact gtcagccctg attgtcggaa
     4321 gccttgtaag tggctatctg caacatacca tttccataaa aacagctctc tatgcctcac
     4381 ttgtcatcca gttgcttctt ctagtaggat ttgccacagt tcgttttgac tggattctca
     4441 tctttagtac cttggatgcc tttttcgcag gtgttctctc tcctagattg caagaactcg
     4501 tctttaaaca aatacctgag gagtcaatgg gagcagttca atcttctatc agtgccatta
     4561 cggttgtttt acctagctta tttacaatat ctttggtaac cattgctact agctttggaa
     4621 ctctggcagt tagctttgtt ttattgctat ttcttctagt tgcctttgtt atgcttctga
     4681 atattcgtga aagtatttag tggagaattt ctataatata tgtttgagac tggattgcca
     4741 gtctttttat gatggtcaaa atcaacagac tttttctctt ttctgaatgt gttatagtag
     4801 actgaatcta aaat
//
LOCUS       DAVLYV010000108         4788 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLYV010000108 DAVLYV010000000
VERSION     DAVLYV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.108"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(52..177)
                     /gene="comC"
                     /locus_tag="VYO70_001962"
     CDS             complement(52..177)
                     /gene="comC"
                     /locus_tag="VYO70_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1714121.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            complement(342..415)
                     /locus_tag="VYO70_001963"
     tRNA            complement(342..415)
                     /locus_tag="VYO70_001963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(379..381),aa:Arg,seq:tct)
     gene            complement(459..938)
                     /gene="rlmH"
                     /locus_tag="VYO70_001964"
     CDS             complement(459..938)
                     /gene="rlmH"
                     /locus_tag="VYO70_001964"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1714122.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            1121..2302
                     /locus_tag="VYO70_001965"
     CDS             1121..2302
                     /locus_tag="VYO70_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1714123.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            2360..3118
                     /locus_tag="VYO70_001966"
     CDS             2360..3118
                     /locus_tag="VYO70_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1714124.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKHQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            3331..4692
                     /gene="dnaA"
                     /locus_tag="VYO70_001967"
     CDS             3331..4692
                     /gene="dnaA"
                     /locus_tag="VYO70_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1714125.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1670 a          857 c          795 g         1466 t
ORIGIN      
        1 caataaccgt cccaaatcca aataaatcca ttactctttc cccttatttc attacttttt
       61 tctttgtaaa ataaaatcac ggaagaattt tgacaacctc atctccccac ctttaatctt
      121 ttgtaagtct ttttccttca aagctacaaa ctgttccaat ttaactgtgt ttttcataat
      181 aaaatctcct aaaatgtttt ttcttgtaag ctaacttaca aaaaccatta tacaaaatgg
      241 aatttcgttt tagataaaat tctctcaact gtcatttttt tctcccaaag tgtacttttt
      301 taagaaaaaa gccgggaaaa ttcccagctt tgctattata ttgatcccag caggattcga
      361 acctgcgacc gttcgcttag aaggcgaatg ctctatccag ctgagctatg agacctaata
      421 caattattct accaaaaatt caattaaaag tcaattttct atttatggta ggggaatccc
      481 tgctgaatcg taaaagcgcg atagatttgt tcaacaagaa ctagtctcat taactgatgg
      541 ggtaaggtta ggcgaccaaa actgacagaa agattggctc tattttttac agatgatgac
      601 aatcctaaac ttcccccaat aataaaagta agagtagaaa atccttttat agaagtttct
      661 tctaactgct tactaaattc ttctgagaag aaagttttcc cttcaatggc taacacaata
      721 acgaaatcac ggtcagcaat ttttgataaa attctctgac cttctatttc taaaatcttt
      781 tgattttctg attcactggc cttatctggt gttttttcat ctgataactc aatcatttca
      841 aacttagcaa atctagaaat tcgttttgaa tactctgcga taccatcttt taaatacttt
      901 tctttcagtt tcccaactgt tacaacttta attttcatga ctctattcta acatattctc
      961 tattttttca catcttattc acaaaataaa aaatagattt caattaagaa aatcacagtt
     1021 tcaaaagagt tatccacagt ttgtgtaaaa cttttgtgtt taagttataa ttaagctagt
     1081 cagtttatac tttcagtaat tcaaacatac ggaggcaaat atgaaacatc taaaaacatt
     1141 ttacaaaaaa tggtttcaat tattagtcgt tatcgtcatt agctttttta gtggagcctt
     1201 gggtagtttt tcaataactc aactaactca aaaaagtagt gtaaacaact ctaacaacaa
     1261 tagtactatt acacaaactg cctataagaa cgaaaattca acaacacagg ctgttaacaa
     1321 agtaaaagat gctgttgttt ctgttattac ttattcggca aacagacaaa atagcgtatt
     1381 tggcaatgat gatactgaca cagattctca gcgaatctct agtgaaggat ctggagttat
     1441 ttataaaaag aatgataaag aagcttacat cgtcaccaac aatcacgtta taaatggcgc
     1501 cagcaaagta gatattcgat tgtcagatgg gactaaagta cctggagaaa ttgtcggagc
     1561 tgacactttc tctgatattg ctgtcgtaaa aatctcttca gaaaaagtga caacagtagc
     1621 tgagtttggt gattctagta agttaactgt aggagaaact gctattgcca tcggtagccc
     1681 gttaggttct gaatatgcaa atactgtcac tcaaggtatc gtatccagtc tcaatagaaa
     1741 tgtatcctta aaatcggaag atggacaagc tatttctaca aaagccatcc aaactgatac
     1801 tgctattaac ccaggtaact ctggcggccc actgatcaat attcaagggc aggttatcgg
     1861 aattacctca agtaaaattg ctacaaatgg aggaacatct gtagaaggtc ttggtttcgc
     1921 aattcctgca aatgatgcta tcaatattat tgaacagtta gaaaaaaacg gaaaagtgac
     1981 gcgtccagct ttgggaatcc agatggttaa tttatctaat gtgagtacaa gcgacatcag
     2041 aagactcaat attccaagta atgttacatc tggtgtagtt gttcgttcgg tacaaagtaa
     2101 tatgcctgcc aatggtcacc ttgaaaaata cgatgtaatt acaaaagtag atgacaaaga
     2161 gattgcttca tcaacagact tacaaagtgc tctttacaac cattctatcg gagacaccat
     2221 taagataacc tactatcgta acgggaaaga agaaactacc tctatcaaac ttaacaagag
     2281 ttcaggtgat ttagaatctt aattgacatc tatgtaaaga aagctttaca taagagaaaa
     2341 gatgtgttag tgtagaatca tggaaaaatt tgaaatgatt tctatcacag atatacaaaa
     2401 aaatccctat caaccccgaa aagaatttga tagagaaaaa ctagatgaac tagcacagtc
     2461 tatcaaagaa aatggggtca ttcaaccgat tattgttcgt caatctcctg ttattggtta
     2521 tgaaatcctt gcaggagaga gacgctatcg ggcttcactt ttagctggtc tacggtctat
     2581 cccagctgtt gttaaacaga tttcagacca agagatgatg gtccagtcca ttattgaaaa
     2641 tttacagaga gaaaatttaa acccaataga agaagcacgc gcctatgaat ctctcgtaga
     2701 gaaaggattc acccatgctg aaattgcaga taagatgggc aagtctcgtc catatatcag
     2761 caactccatt cgtttacttt ccttgccaga acagattctt tcagaagtag aaaatggcaa
     2821 actatcacaa gcccatgcgc gttccctagt tgggttaaat aaggaacaac aagactattt
     2881 ctttcaacgg attatagaag aagatatttc tgtaaggaaa ttagaagctc ttctgacaga
     2941 gaaaaaacaa aagaaacatc aaaaaactaa tcatttcata caaaatgaag aaaaacagtt
     3001 aagaaaacta ctcggattag atgtagaaat taaactatct aaaaaagaca gtggaaaaat
     3061 cattatttct ttttcaaatc aagaagaata tagtagaatt atcaacagcc tgaaataagg
     3121 ctgttctttt atttttttat ctcacaaggt tatccactat gtttttcgat aaaaagctta
     3181 ataaatcaat aatttcttct tttatcccca acctgtggat aaagtttggt aacattgtgg
     3241 attatttttc acagcttgtg gaaaattctt gctatctatg gtaaaatatc tctagtatta
     3301 aacttttaaa tagtaaagga ggagaaagga ttgaaagaaa aacaattttg gaatcgtata
     3361 ttagaatttg cacaagaaag actgactcga tccatgtatg atttctatgc tattcaagct
     3421 gaactcatca aggtagagga aaatgttgcc actatatttc tacctcgctc tgaaatggaa
     3481 atggtctggg aaaaacaact aaaagatatt attgtagtag ctggttttga aatttatgac
     3541 gctgaaataa ctccccacta tattttcacc aaacctcaag atacgactag ctcacaagtt
     3601 gaagaagcta caaatttaac tctttataac tatagtccaa agttagtatc tattccttat
     3661 tcagatacgg gattaaaaga aaagtatacc tttgataact ttattcaagg ggatggaaat
     3721 gtttgggctg tgtcagccgc tttagctgtc tctgaagatt tggctctgac ctataaccct
     3781 ctttttatct atggaggacc aggccttggt aagactcact tattaaacgc tattggaaat
     3841 gaaattctaa aaaatattcc taatgcgcgt gttaaatata tccctgccga aagctttatt
     3901 aatgactttc ttgatcacct aagacttggg gaaatggaaa agtttaaaaa gacctatcgt
     3961 agtcttgatc ttttgttaat cgatgatatc cagtcactca gcggaaaaaa agtcgcaact
     4021 caggaagaat ttttcaatac ctttaacgcc cttcatgaca agcaaaaaca gattgtccta
     4081 acgagtgatc gtagtccaaa acatctagaa gggctcgagg agaggcttgt cacgcgtttt
     4141 agttggggat tgacacaaac tatcacaccc cctgactttg aaacacgtat tgccatttta
     4201 caaagtaaaa cggaacattt aggctacaat ttccaaagtg atactctaga atacctagct
     4261 gggcaatttg attcaaatgt tcgagatctt gagggagcca tcaacgacat cactttaatt
     4321 gccagagtaa aaaaaatcaa ggatatcact attgatattg ctgcagaagc cattagagcc
     4381 cgcaaacaag atgttagcca aatgctcgtc atcccaattg ataaaatcca aactgaagtt
     4441 ggtaactttt atggtgttag tatcaaagaa atgaagggaa gtagacgcct tcaaaatatt
     4501 gttttggccc gtcaagtagc catgtattta tctagagaac taacagataa tagtcttcca
     4561 aaaattggga aggaatttgg gggaaaagat cataccacag tcattcatgc ccatgccaaa
     4621 ataaaatctt tgattgatca agacgataat ttacgtttag aaattgaatc aatcaaaaag
     4681 aaaatcaaat aatttgtgga taacttttag ttttttatct tttttatcca cattttttaa
     4741 acaagctaaa aaacttgata tgacttgttt aaaggctgtt ttccacag
//
LOCUS       DAVLYV010000109         4614 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLYV010000109 DAVLYV010000000
VERSION     DAVLYV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.109"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            2..262
                     /locus_tag="VYO70_001968"
     CDS             2..262
                     /locus_tag="VYO70_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002878647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1714126.1"
                     /translation="MKKGNPKSDRLQEIADYFGVSTDYLLGRTENPNLADDTKEYIWQ
                     GKVLNVEEMASNVMMFGGRELTDEKKKIIQSIIEGYLKEAGD"
     gene            275..658
                     /locus_tag="VYO70_001969"
     CDS             275..658
                     /locus_tag="VYO70_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEV1714127.1"
                     /translation="MTEKEIISHFQVRIVDFDGELIPDELGFYEKETNTAFLSNKLSK
                     KERVKVLLHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPKEFDYLK
                     FMSYYNLKTVTNEVMVKEEYQTLIG"
     gene            703..1110
                     /locus_tag="VYO70_001970"
     CDS             703..1110
                     /locus_tag="VYO70_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HEV1714128.1"
                     /translation="MALFGKKQDESLEVELFTEEPNERVFEFKKSKTVVRIDDYFIRI
                     ARKSNVSNVLLHGLDGEKSILLSEITAYQLKEPGSTVGYLQLVYPGSSDTKGGVFDAV
                     KDENTVTFTKDEKASILELKKAIEKALKDKVKK"
     gene            1481..2629
                     /locus_tag="VYO70_001971"
     CDS             1481..2629
                     /locus_tag="VYO70_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1714129.1"
                     /translation="MKYNKTKYPNIFYYETAKGKRYYVRRSFFFRGKKREKSKSGLTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSTLLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(2871..4157)
                     /locus_tag="VYO70_001972"
     CDS             complement(2871..4157)
                     /locus_tag="VYO70_001972"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1714130.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(4388..4600)
                     /locus_tag="VYO70_001973"
     CDS             complement(4388..4600)
                     /locus_tag="VYO70_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1714131.1"
                     /translation="MANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVRYGIGCRKD
                     FIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         1638 a          940 c          795 g         1241 t
ORIGIN      
        1 tctgaaaaaa ggtaacccaa aatctgatag attacaagaa atagcagatt attttggtgt
       61 tagtacagac tacttactag gccgtactga aaatcctaat cttgccgatg atacaaaaga
      121 gtacatatgg cagggcaaag ttctcaatgt tgaagaaatg gcatctaatg tcatgatgtt
      181 tggtggccga gaattaacag atgaaaagaa gaaaatcatc cagtctatca ttgaaggtta
      241 tctcaaagaa gctggtgatt agaggtactg cttagtgacc gaaaaagaaa ttataagtca
      301 ttttcaggtt cgcattgtcg attttgacgg cgagctaata cctgatgaac ttggatttta
      361 cgaaaaagaa accaatacag ctttcttgtc taataaactc agcaaaaaag agagagttaa
      421 ggtcctactt catgaactag gacacaagga tcacacacga tcagaatacc agaacgctcg
      481 cctacgctgt gaaaacgaag ctgataggaa tatgatccat catctcgtaa aagacgcact
      541 agaaagctta gacgacccca aagagtttga ttacctcaaa ttcatgtctt attacaatct
      601 taaaaccgtg acaaatgaag tcatggtaaa agaggaatat cagactttaa ttggttaaat
      661 atgtttataa actgctgaag cagaaaaaga aaggaactac ttatggcatt gtttggtaaa
      721 aagcaagatg aaagtttaga ggttgaactc ttcacagagg aaccgaatga gcgagttttt
      781 gagtttaaga aatcaaaaac tgttgtaaga atcgatgatt attttatcag gattgcaaga
      841 aagtcaaatg tatctaatgt tcttcttcat ggtcttgatg gcgaaaagtc aattctcctc
      901 tctgagatta cagcatacca attgaaagaa cctggctcaa ctgttggcta tcttcaactt
      961 gtttaccctg gttcttctga tacaaaaggt ggtgtgtttg atgccgtaaa agatgaaaac
     1021 acagtaacct ttaccaaaga tgaaaaagca tctattttgg aattaaagaa agccatagag
     1081 aaggctttaa aagataaagt caagaaataa aaaaagcccc acactctcta tctctaaatt
     1141 ctgagtgtga agactcaact ttccattttt gacaaaatga aatatatttg ataatatata
     1201 gttactaacc taggggaaat cctagtgcaa ataacctggt tggcacaagc tgccacgcag
     1261 aaacggtaac tataaattta gttaccgttt tttgttgaat taaaaacaaa aaagccccac
     1321 aatcgccctc gccaaagttt gattgtgaag cttagcctta taagaaatca gccattaaaa
     1381 aggcctattt tctataccct attttacacc atgaaagggg tgatgtcaat attctcaatg
     1441 tttagacctt gtccagaagc cgataaacaa ggagaataca atgaaatata ataaaacaaa
     1501 atacccaaat atcttttact atgagactgc caaaggcaag cgatactatg tcagacgttc
     1561 ttttttcttc cgaggtaaaa aaagagaaaa aagtaaaagt ggtctcacaa cccttccaca
     1621 agctcgtgca gccttagtag agcttgagca acaaatccaa gaacaagaat taggtatcaa
     1681 tacgaatctg acacttgatc aatattggga tatttattct gaaaagagat tgtcaacagg
     1741 gcgctggaat gacacttcct actacctcaa tgataatctc tacaagaacc atatcaaggc
     1801 aaagtttggt tctaccctgc ttaaaaattt ggatagaaat gagtatgaac tctttatcgc
     1861 tgaaaagttg cagaaccata ccagatacac tgttcaaacc ctcaattcca gcttcatggc
     1921 attgctgaat gatgccgtca aaaatggaaa tctgctctca aatcgcttga aaggtgtttt
     1981 catcggccag agtgatatcc ctgctgcaaa caagaaagtg actctcaaag agttcaagac
     2041 ttggatagca aaggcagaag agattatgcc aaaacaattc tacgctctga cctatctgac
     2101 aatttttgga ttgagaagag gagaagtctt tggattgcgt ccaatggaca tcactcagaa
     2161 cgacagcgga cgggctatac tgcatcttag agacagtcga agcaaccaga ccttaaaagg
     2221 gaaaggaggg cttaaaacga aggattcaga gcgatatgtc tgccttgatg atatcggaac
     2281 agacctgatc tattatctga tagctgaagc ttctaagatt aagcgaaagt taggaattat
     2341 caaggaacag cacaaggatt atataactat caacgagaaa ggtggtctca tcaatccaaa
     2401 tcagctaaat agaaacttca atctagtgaa tgaagcaaca ggattgcatg taacacctca
     2461 catgatgcgc cacttcttca cgactcaaag cattattgca ggggttccgc ttgaacaatt
     2521 aagccaggcg ctggggcata caaaggttta tatgacggat cgttacaatc aagtagagga
     2581 cgaacttgct gaagcgacaa cagacctatt tcttagtcat attcgctaaa aaagtccccg
     2641 ccaattcccc gaccaaaatc cgaaaaatac cgaaaaatat cgaaaaatta tttttagaat
     2701 agtccccaaa agtctgaaat aaagctaaaa aactccacct gattgggtgg agttaaggga
     2761 gattattatg aaaaagaaaa gtttaggata tttgttacaa caagttagga ggtcttcttg
     2821 taaccgtcta tagtataccc gacctatctt aaacaaatct taaaaatctc ttaggaccaa
     2881 acactttcta aaatatttgt ttgttcacga ccaggaccta ctgagaaagt agaaatacga
     2941 acgccaacca attcactcac acgacgaaca tagttacgcg cattctcagg aagatcttcc
     3001 aaattgcgaa ctccggtaat atcttctgac caacctggta actcttcata gataggcttg
     3061 caacgtttca attgctcaag actagctgga tagtagtcaa tacgttgacc gtcaagatca
     3121 taggccacac agattttcac agtatccaaa ccgctcaaaa catcaataga gttcaaagaa
     3181 aggttagtaa taccagaaac acgacggcta tgacgcatca caactgagtc aaaccaacct
     3241 acacgacgtg gacgaccagt tgttgtacca tactcatgac ccacttcacg gatacgttct
     3301 cccacttcat caaacaactc agttgggaaa ggaccatctc ctacacgact cgtataagct
     3361 ttacatacac ctacaacctt gtcaatcttg cttggaccga caccagaacc aattgtcaca
     3421 ccaccagcta cagggtttga tgacgtaaca aatggataag taccttggtc gatatctagc
     3481 ataacacctt gtgcaccttc aaaaagcaca cgtttgccat tatcaagcgc atcattcaag
     3541 ataacagatg tatctatcac gtatttcttg atttgttgac catattcgta atattcttca
     3601 aaaatatcat cgaaaacaat cgctttactg tcatacaatt tttcaaaaag acgattcttt
     3661 tcagcaaggt tacgttctaa acgctcacgg aaaatatctt tatctaaaag atctgcaata
     3721 cgaattccaa cacgagcagc cttgtccata taagctggac caattccctt aattgtcgta
     3781 ccaatcttat tgtcgccctt agcttcttct tgcaagcgat ccaactcgat atgataaggc
     3841 aaaataacat gcgcacgatc agaaatacgc aagttatcag ttgttacacc ttcctcatga
     3901 agatagctca actcttttac aagagattta ggatttacaa ccataccatt cccaatgaca
     3961 gatatttttt cagggaagaa aatcccagat ggaatcaagt gcaacttaaa tttcttaccg
     4021 tcaatcacaa tcgtgtgacc agcattatca ccaccttggt aacgtgcaat cacttctgca
     4081 ttcgctgaaa ggaagtctgt aatcttccct ttaccttcat caccccattg ggtacctaca
     4141 acaacaactg aagtcataat tttgtctgag ccctcaggct cttcctttct cacatacatg
     4201 gcaggactct cacctgcaat tatatcttac aatttattat aataaaaaat cgccttttta
     4261 tcaagaagaa acaatagaaa gatttgctat ttccaactat taaaaaatga tttagaaaaa
     4321 ttactagcta tttactatta tctttccata aaagagtaaa ttagttcgga aatttactaa
     4381 aattacctca acaagaaata aaaccccgat tcattaccaa tttttcaaga tacaaacgat
     4441 aagcaacacg ataatggtaa acaataaaat ccttacgaca accaatgcca tatctcacta
     4501 aataataaat taaaaattta aaatgaacat gttcccagtc aaaattatca ccaaatgtag
     4561 gaccatactc ttcttcaata ctatcataga aattagccat ctgctcataa attt
//
LOCUS       DAVLYV010000110         4607 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLYV010000110 DAVLYV010000000
VERSION     DAVLYV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.110"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(56..1066)
                     /locus_tag="VYO70_001974"
     CDS             complement(56..1066)
                     /locus_tag="VYO70_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1714132.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VYO70_001975"
     CDS             1215..2384
                     /locus_tag="VYO70_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1714133.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQAISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VYO70_001976"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VYO70_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1714134.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQNLTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VYO70_001977"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VYO70_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1714135.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPMRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTIDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLSGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VYO70_001978"
     CDS             4146..4379
                     /locus_tag="VYO70_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1714136.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            4425..4607
                     /locus_tag="VYO70_001979"
                     /pseudo
     CDS             4425..4607
                     /locus_tag="VYO70_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1356 a          881 c         1173 g         1197 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtattaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaatgagga atttagagag ttgtttgaga ggggtaaaga
      241 aactactaga cacaccgaga gaggtcagaa gggtggttag gagcgatgca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggataga aagagcgtga tagggataat ggccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggcta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcgg
     1441 ccagtgattt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaatcttgg
     1501 tcactatcgg agcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcgaccttca
     1981 cagcccagtt aatcaagagt caccagtact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaacgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaaat ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tatgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggacttatgt caacattgcc gactatagat
     3541 gggaaaggtt ttgacatgct tgacctcggt gctaatgcag aaaatacagc ccagcacctc
     3601 catcaatacg ctgtcctagg ttctttctat gccaagaatg ttcgtggcat tgcgcaacca
     3661 cgtgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagtggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgccagatt
     4081 cgtaccatgc tagaaacaga cgtagttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aagaaattga ccaactccaa aacgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggaaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
     4501 aaactccacg taaatccagc aaactcaagc cgctaacagt tgaagataaa gcctgtaatc
     4561 atgcgctatc taaggagaga agcaaggttg agaatatctt tgccaaa
//
LOCUS       DAVLYV010000111         4590 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLYV010000111 DAVLYV010000000
VERSION     DAVLYV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.111"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..241
                     /locus_tag="VYO70_001980"
     CDS             <1..241
                     /locus_tag="VYO70_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1714137.1"
                     /translation="LHEKGLLIWVNALTLSDSIILSAKIDDDTAIAHDGESWGKLVSI
                     GFDIIQTDWPLLLYQYLVEKNKKIKIKENYHVQKL"
     gene            225..1286
                     /locus_tag="VYO70_001981"
     CDS             225..1286
                     /locus_tag="VYO70_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1714138.1"
                     /translation="MFKNFKKIAFTSSLILLAACSNNASNTNQSTDEGSVDKSQEIVI
                     YTNSASNGRADWLQTKASEQGYNLRLVDISGGELADRLIAEKNNAVADMVIGLNKLEF
                     NRIKAEKLLVKYSPKWADEVDSSLGDSEGYYSPIVNQPLVLIGNKDAKMPSDWTDFTD
                     SSYKGKYGISKLSTGTSKNIFASIVSRYKDEKGELGISDEGWKVAKAYLKNAHVYAEG
                     EDYISSIMDNNNDLKYSMMWGSGVLQNQKEREYKFQVMSPKVGVPYVTEQVGILNTSK
                     KQALLKEFVDWFGSSELQKEWSDKFGSIPANKKALEQAKDELKQFANSVKPQELDWEF
                     IGKNIDSWIEKAQLEFVEQ"
     gene            1303..2307
                     /locus_tag="VYO70_001982"
     CDS             1303..2307
                     /locus_tag="VYO70_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714139.1"
                     /translation="MITFKDIQIKYNDFIAIDNLNLTINEGEFFTFLGPSGCGKSTTL
                     RALVGFLEPSQGQILVDNQDITNLDPEKREIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VSPEIIKAKVHEIAKKINISDEQLNRNISELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVSLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGFIEQVGTPY
                     DIYNQSATQFVCDFIGSINKISQEVLKKLQGENKYFKETEGYIRLERVKLEKTSPQDL
                     SITGVISDIEFNGVLIKYKVDLSNSESIHIIEKNDGLSRHIIKEKVTMYINPRDIMQF
                     "
     gene            2319..4013
                     /locus_tag="VYO70_001983"
     CDS             2319..4013
                     /locus_tag="VYO70_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1714140.1"
                     /translation="MRKSLNWKDCLIRLGVLWLIFALIIYPNIELLSGVFYKNGSFTL
                     EAFNKIIHSQRAMSSIFNSFILAISMVFTVNIVGTLIVLLTEYWDIRGSKILKLGYMS
                     SLVYGGIVLATGYKFVYGANGLVTKTLSSIIPGLAPNWFQGFGAVLFIMTFACTSNHI
                     MFLTNAVRSVDYHTIEAAKNMGASPLKVFFSVVFPTFKPTLFALTILTFLTGLSAVSA
                     PLIVGGKDFQTINPLIISFAKTTSSRDLAALLAIMLGLATILLLTLLNHVEKGGNYVS
                     VSKTKASIKKQIISSPLWNTLAHIVAYVLFAIYMLPIVLIILYSFTDSLTIKTGTLDL
                     SKFTLANYANLFTDAQSFRPYLVSVVYAILAAVIATIIAIVISRIVHKNKSKFDKFFE
                     YGALIPWILPGTLIALGLMFTYNIPHLILFNLVLVGTVIILLIAYTIQKLPFSYRMIR
                     AVFFSIDNDMEEAARSMGASSFYTMVRVIIPYILPVVLSVVVLNFNSLLSDYDLSVFL
                     YHPLFQPLGIVIKQSTDETATLNAQAMMFVYSVILMIMSSAALYLSSLFQGKRGKR"
     gene            4015..>4590
                     /locus_tag="VYO70_001984"
     CDS             4015..>4590
                     /locus_tag="VYO70_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1714141.1"
                     /translation="MNLSYELSIWEILLRCFLAILFGGIVGYERGVKNQAAGIRTYLL
                     VCLGTCVVMMTNQFITSRYGSGDPSRMGAQVISGLGFLGAGTIMVTNQKVKGLTTAAG
                     LWSSATIGLAIGIGFYEGAILAGVVLIAVLTLFTPLKDYLNSKTKIADYYLVFSSLEG
                     YNRLLIYLNTSGIKILEIQNGIGNLDEERQLN"
BASE COUNT         1528 a          650 c          854 g         1558 t
ORIGIN      
        1 tttacatgaa aaaggattac ttatttgggt taatgcttta accttaagtg attcaattat
       61 tctttcggct aagattgatg atgatacagc tattgctcat gatggcgaat cttgggggaa
      121 attggtttca ataggatttg atattattca aacagattgg ccattattac tttatcaata
      181 tctagttgaa aaaaataaaa aaataaaaat aaaggagaac tatcatgttc aaaaacttta
      241 aaaagattgc attcacttct tcactaattt tacttgctgc ttgctcaaac aatgcttcaa
      301 atacaaatca gtctactgat gaaggaagtg ttgataaatc tcaagaaatt gtaatctata
      361 caaattcggc ttctaatgga cgtgcagatt ggttacaaac taaagcatca gagcaaggat
      421 acaatcttcg tttggtagat atttcaggtg gagaactagc tgaccgtttg attgcagaaa
      481 agaataatgc tgtggctgat atggtaattg gattaaataa attagaattt aaccgtatta
      541 aagctgaaaa acttcttgta aaatactcac caaaatgggc tgatgaagta gatagctctt
      601 tgggagattc tgaagggtat tattcaccaa ttgtaaatca accattagtt ttgattggta
      661 ataaagatgc taaaatgcca tccgactgga cagattttac tgattcatct tataaaggta
      721 aatatggtat ttcaaaatta agtacaggta catctaagaa catctttgca agtattgttt
      781 cgagatataa agatgaaaaa ggtgagttag gaatatctga tgaaggttgg aaagttgcaa
      841 aagcttacct taaaaatgca cacgtttatg cagaaggtga agactatatc tcttcaatta
      901 tggataataa taatgatcta aaatatagta tgatgtgggg atctggtgtt ctacaaaatc
      961 aaaaagaacg tgaatataaa ttccaagtaa tgtcaccaaa agttggtgtt ccttatgtaa
     1021 ctgaacaagt tgggatttta aatacaagta agaagcaagc attattaaaa gaatttgtag
     1081 attggtttgg ctcttcagaa cttcaaaaag aatggtctga taaatttggt tcaattccag
     1141 ctaataaaaa agctctagag caagcaaaag atgaactcaa acagtttgct aatagtgtta
     1201 aaccacaaga acttgactgg gagtttatcg ggaaaaacat cgattcttgg attgaaaaag
     1261 cacagcttga atttgttgaa cagtaatagt taggattaag tgatgattac atttaaggat
     1321 attcaaatta agtataatga ttttattgcc atcgataatc tcaatttaac aatcaatgag
     1381 ggagagtttt tcacattttt aggaccaagt ggttgtggga aatcaacaac tcttcgtgca
     1441 ctagttggat ttttggagcc ttcacagggg caaatactag ttgataatca agatattacg
     1501 aatttagatc cggaaaaacg cgaaattggt attgtgtttc aaagttatgc attattccca
     1561 acaatgactg tttttgataa tattgctttt ggtctaaaag ttaaaaaagt ttctcctgaa
     1621 attattaaag ctaaagttca tgaaattgcg aaaaaaatta atatctcaga cgagcaacta
     1681 aaccgaaata tttctgaatt atcaggggga caacaacagc gtgtagcttt agcacgtgca
     1741 ttagtattag aaccaaaaat tctttgtttg gatgaaccgt tatcaaacct agatgccaaa
     1801 ctacgagttt cgttgagaaa agaattgaaa cgattgcaaa aagaactagg aatcactact
     1861 ctttatgtca cacatgatca agaagaagca ttaacattat cagataggat tgctgtgttt
     1921 aataatggat ttattgagca agttggcacg ccgtatgata tttataatca atccgcaact
     1981 caatttgtct gtgattttat tggctcaatc aataaaatta gccaagaagt tcttaaaaaa
     2041 ttacaaggcg agaataaata ctttaaagaa actgaaggat acatacgctt agagcgtgtt
     2101 aagttagaga aaacttcacc tcaagattta tctataacag gggtaattag tgatattgag
     2161 tttaatggtg tacttattaa gtataaagta gacttgtcga atagtgaaag tattcatatt
     2221 atcgaaaaga atgatggact atcacgtcat ataatcaaag aaaaagtcac tatgtacatt
     2281 aatccgcgtg atataatgca attttaagga gattgtgcat gagaaagagt ctaaactgga
     2341 aagattgtct cataagatta ggagttttat ggctaatctt tgccctgatt atttatccaa
     2401 atattgaact tttgtctgga gtgttttata aaaatggttc atttactcta gaggcattca
     2461 ataagattat acactctcag agagcgatga gtagtatatt taatagcttt attttggcga
     2521 tttcaatggt ctttactgta aatattgttg ggacattgat tgttctactc acagagtact
     2581 gggatattag gggttcaaaa atacttaaat taggttatat gagctctcta gtatatggag
     2641 gaattgtatt agcgacagga tataaatttg tttatggcgc taatggtctt gtaactaaaa
     2701 cgctctcaag tataattcca ggtcttgccc ctaactggtt tcaaggattt ggtgcagtat
     2761 tgtttattat gacatttgct tgtacctcaa atcacataat gtttttaaca aatgcagttc
     2821 gtagtgttga ttatcataca attgaggcag ctaaaaatat gggtgcaagt cctttaaaag
     2881 ttttctttag tgtggttttt ccaactttta aacctacgtt atttgcctta accatcttaa
     2941 cttttttgac aggtctttca gcagtttctg ctcctttgat agtagggggg aaagattttc
     3001 aaactattaa cccattgatt atcagtttcg ctaaaactac aagttcgaga gatttagcag
     3061 ctttactagc tatcatgtta ggtttagcaa ctattctatt attgacactt ttgaatcatg
     3121 tcgaaaaagg aggtaattat gtatctgttt cgaaaaccaa ggctagtatt aagaagcaga
     3181 taatttcttc tccattatgg aatactcttg ctcacattgt tgcgtatgta ttgtttgcaa
     3241 tctatatgtt accaatagtt ttgataattc tatactcgtt tacagactcg ttgacgatca
     3301 agacgggaac tttagatcta tctaaattta cgttagcgaa ctatgctaat ttatttacag
     3361 atgctcaatc atttcgtcct tacctagtca gcgttgttta tgcaatttta gctgcggtaa
     3421 ttgctacaat tatagcaata gtaatttcac gtattgtaca taaaaacaag agtaagttcg
     3481 ataaattctt tgagtatggt gcactgatac catggatatt gccaggtaca ttgattgcac
     3541 taggattaat gttcacttat aatatacctc atttaatact atttaattta gttttagtag
     3601 gaacagtaat tattttactt attgcatata ctattcaaaa attaccattt tcttatagaa
     3661 tgattcgggc tgtatttttc agtatagata atgatatgga agaagctgca cgtagtatgg
     3721 gtgcatctag tttctacact atggtgcgag ttattatacc gtatatttta ccagtcgtat
     3781 tatcagtagt tgtgttaaac tttaattctt tattatctga ctatgactta tctgtattct
     3841 tgtaccatcc acttttccaa ccattgggaa tcgttattaa acagtctact gatgaaacag
     3901 caactttaaa tgcacaggca atgatgtttg tttattctgt aattttaatg attatgtcta
     3961 gtgcagctct ttatttatca agcctattcc aaggtaaacg aggtaagaga taatatgaat
     4021 ctttcatacg aactgagtat ttgggaaata cttcttaggt gctttttagc gattcttttc
     4081 ggtggcattg ttggctatga gcgaggagtc aaaaaccaag cagcagggat tagaacctac
     4141 ttactagttt gtttgggaac ttgtgttgta atgatgacaa atcagttcat cactagtcgt
     4201 tatggtagcg gtgatcctag tcgaatggga gcccaagtaa ttagtggctt gggtttccta
     4261 ggggcaggaa caatcatggt aactaatcaa aaagttaaag gtttaacaac agcagctgga
     4321 ttatggtctt cagcaactat tggtttagct ataggcattg gtttttatga aggtgctatt
     4381 ttagccggag ttgtactcat agcagtttta actctattta ctcctcttaa agattattta
     4441 aactcgaaaa ctaaaattgc tgattactac ttggttttta gttctttaga aggttataat
     4501 cgtttactta tatatttaaa tacatcgggt attaagattt tagaaattca aaatggtata
     4561 ggaaatcttg atgaagaacg tcaattaaat
//
LOCUS       DAVLYV010000112         4448 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLYV010000112 DAVLYV010000000
VERSION     DAVLYV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4448
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.112"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            76..441
                     /locus_tag="VYO70_001985"
                     /pseudo
     CDS             76..441
                     /locus_tag="VYO70_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            465..683
                     /locus_tag="VYO70_001986"
     CDS             465..683
                     /locus_tag="VYO70_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714142.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     IAHYGRVETAISFLPSSMEIEPTDESGT"
     gene            complement(740..1024)
                     /locus_tag="VYO70_001987"
     CDS             complement(740..1024)
                     /locus_tag="VYO70_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1714143.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(1078..2013)
                     /locus_tag="VYO70_001988"
     CDS             complement(1078..2013)
                     /locus_tag="VYO70_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1714144.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNITDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKVHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            2117..2560
                     /locus_tag="VYO70_001989"
     CDS             2117..2560
                     /locus_tag="VYO70_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1714145.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            2676..2942
                     /locus_tag="VYO70_001990"
     CDS             2676..2942
                     /locus_tag="VYO70_001990"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1714146.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREAVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            2939..3313
                     /gene="crcB"
                     /locus_tag="VYO70_001991"
     CDS             2939..3313
                     /gene="crcB"
                     /locus_tag="VYO70_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1714147.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIYLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYLSLI
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            3307..3636
                     /gene="crcB"
                     /locus_tag="VYO70_001992"
     CDS             3307..3636
                     /gene="crcB"
                     /locus_tag="VYO70_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1714148.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            3755..4102
                     /gene="rplS"
                     /locus_tag="VYO70_001993"
     CDS             3755..4102
                     /gene="rplS"
                     /locus_tag="VYO70_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1714149.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            4144..4215
                     /locus_tag="VYO70_001994"
     tRNA            4144..4215
                     /locus_tag="VYO70_001994"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4177..4179,aa:Arg,seq:cct)
BASE COUNT         1303 a          789 c          964 g         1392 t
ORIGIN      
        1 gaaactagaa caggacaaat cgatttctaa caatgtttta gaagtagagg tgtactattc
       61 tagtttcaat ctactatatt tcgtctgatg ggcaaatctt ataaagagat tatagagctt
      121 ttagactgta atcaaacaac gatttggcga aacgtaaaaa aatatgagga gttcggactc
      181 gactctctcc ttcttactcg ttatggacaa tgctatatgg cataaatcaa gtaccttaaa
      241 tattccgact aatattgatt ttacctttat tcctccatac acaccagaga tgaacccatt
      301 gaacaagtgt ggaaagagat tcgtaaacgt ggatttaaga ataaagcctt tcgaactttg
      361 gaagatgtca tgaatcaact tcaagatgtc atacaaggac tggagaagga ggtgataaag
      421 tccatcggta atcggagatg aactagaatg ctttttgaaa gcagatgagt ataagtcctc
      481 gttttgaaac cttagagcag gccattgcct ccaaagattt ggaaaaagtc cgagaagctt
      541 ttaagaaaat gaattccact tggaccatca atgaaagtgt ggttcgtgac aatagtatag
      601 ctcattatgg acgtgttgaa acagctattt ccttcctgcc tagtagcatg gaaatcgaac
      661 ctacagatga gtctggaaca tgaaaacagc agttgatttc aagaaacaaa aaagaacagc
      721 caaaactgtt ctcttatcat tatttgagac cgtatttttt gttgaaacga tccacgcgtc
      781 catctgcttg agtgaacttt tgacgtccag tgtagaatgg gtgtgagtct gatgaaattt
      841 ccacacggat caatgggtaa gtttcgcctt cgaactcaac tgtttcgtta gagcgttttg
      901 ttgaaccgct aaggaattgg taaccagtag ttgtgtccat gaagacaact gggcgatatt
      961 ctggatggat atcttttttc attttgcaat atttcctttc tgccatggtc tctttgcgag
     1021 ccatagattg ttactatact agtctatcag attctgaaaa gtttggcaag tattttatca
     1081 gtttttaagc aagtttttta acttttggta gatgatttcg ttttcttcta ggctatagga
     1141 attagcacca cttgctagag ggtggcctcc accatcatgc tccttggcaa tttcattgat
     1201 aggatggact ttactgcgta agcgaactcg gtagtggcca tcagcctgtt cgacaaaaat
     1261 tccccagaga ctcactctgt caatgcgtcc aggtgcacct acaatggccg cagtttcagc
     1321 atcggttata ttgtattgtt tcaagatttt ctgactcagg ataacgcgag cagcaccatt
     1381 ttcatccact tccagatggt cgtagatgta gccttgcagt ttagcaattt tgtagctcat
     1441 agtgtccatt ttgcgagtga gagccgcaaa gtcaaagtta tgttctctca aataagcagc
     1501 caggcgaaga gtccgtgcag tggtagaagg gtagaggaag cgacctgtat caccgacaat
     1561 tcctgcaaag agcaactcag catcgcgatc tgccaaggct agttgggttg tttgggcaaa
     1621 tagggtaatc atctcgctag cgctacttga actagtatcg acccaagaca ggtcaccgta
     1681 tacatcatca tttggatggt ggtcaatctt aatgagaaaa tcaccttgac tatagcgctt
     1741 atcatcgata cgagcagtat tagctgtatc acagacgatg acaagtgcgc cttggtaggc
     1801 tctatcttca acaagatcca tctcagccat ccaagtaaga gttggttcat caaaaccgac
     1861 ggctttgatg gttttttctg ggaaatgatg ttctagcaag gctttcaatc ccacctgact
     1921 tcccaaggca tcagggtctg gtttcatatg acgatgaatg ataatggtgt cgtattcttt
     1981 aattttttct aaaatttgtt ggcaaatctc cataaataac ctcaattctt tctcatttca
     2041 ttgtagcaca agaattttta tttttaaaat gaaaaagatg tgatataatg gagaaagata
     2101 aattgaggag gacgatatgg cattagcaaa aattgtattt gccagtatga ccggtaatac
     2161 cgaagaaatt gcagatattg tagcagacaa attacgtgac ttgggcttgg atgttgatgt
     2221 tgatgaatgt acaactgttg acgcttcaga cttcttggaa gcagacatcg ctatcgttgc
     2281 gacctatact tatggtgatg gtgaattgcc agatgagatg atggacttct acgaagacct
     2341 agcagatctc aacttgaatg gcaaaatcta cggagtggtc ggctcaggag ataccttcta
     2401 cgatgaattt tgtaaggctg ttgatgactt tgaccgtgtc tttgtgtcaa caggagcaga
     2461 aaaaggttca gagtgtgtta aagttgatct ttctgccgag gaagaagata ttgaacgctt
     2521 ggaacaattc gcagaagaat tggctgctaa agtaggataa gcataaaagt gcgctgatgt
     2581 aatcaatcag tgcatttttc ttatcgagag ttgggatttt actagcctaa ttgatggctt
     2641 tttgatacaa taattcaaat aaaggaggag actgtatgga tttagatatt attcgtcaag
     2701 aaatcgatca aatcgacgac caaattgtca aactgttaga agaacgaatg cacttagttg
     2761 aaggagttgt cgcttataag aaggcttcag ggaaaccgat tttagatact aagagagaag
     2821 cagttatttt tgaaaaggtt agaagtcgtg tagaggacaa gcgctatcag gagactattg
     2881 tcgcgacttt ttcggacata ctcaaacgtt cgcgtgatta tcaggatcaa aatatcaaat
     2941 gaaaaaagaa caattttatc cgctagggat ttttctagct gctatgttgg gcggacttgt
     3001 ccgctaccta gtttccactt ggttaccagc cagtccagat ttcccttggg gcactctctt
     3061 tgtcaactat ctgggaattt tctgcttgat ttatcttgtc aagggctatc tggtctataa
     3121 ggggactagt aagggcttga ttttagcact ggggacgggt ttttgcggag gtttaacaac
     3181 tttttccagc ctaatgcttg atactgtgaa gctgcttgat acagggcgtt atcttagttt
     3241 gatactgtat ttgcttttga gtatcggtgg aggcctgctt ttagcttact atttggggag
     3301 gaagaaatgg taatcgtcta tcttgcaatc gcctgtggct tgggagcact cgtacggtat
     3361 ttcttttctc gctataatca agcttctaaa ttgccactcg gaacgctcat agctaatctt
     3421 ttaggttgtt ttttaattgg agtattctac aatcatgtag agtctaagga agtatatgct
     3481 attctagcaa caggattttg tggcggttta acaacttttt cgaccttgaa tgacgaactt
     3541 caaagactgc taagtgataa gaaggtcttt tattcttacc tgactttgac ttacataggt
     3601 ggtttggttg cgattttttt aggaattttg ctataaattt ctttactttt ttgtgaaaat
     3661 ttggtaaact gtatcttgtg tgtaatggac gcacaagaat acagtttatc cgctgaggaa
     3721 gtttcctcaa gattgacaaa gataggagaa taaaatgaat ccattaatcc aaagcttgac
     3781 tgaaggtcaa cttcgtacag atatcccatc attccgtcct ggtgacactg ttcgtgtaca
     3841 cgcgaaagtt gtcgaaggta accgtgaacg tatccagatt tttgaaggtg ttgttatcgc
     3901 acgtaaaggt gctggaatct cagaaaacta cacagttcgt aaaatctcta acggtgtagg
     3961 tgttgagcgt atcttcccaa tccacactcc acgtgttgaa aaaatcgaag ttgttcgtta
     4021 cggtaaagta cgtcgtgcga aattgtacta cttgcgtgct cttcaaggta aggcagctcg
     4081 tatcaaagaa atccgtcgtt aattcgacta aaaaactctg cttttcagca gagtttttat
     4141 ctagctccct tagttcaatg gatataacaa ctccctccta aggagtagtt gcaggttcga
     4201 ttcctgcagg gggcatgtaa ttagatatca aagaagtttg tcaaaaagtc tttgatatta
     4261 aaagaacgca taatataggg gattgtctcc ataatattat gcgttttatt attgaaaaat
     4321 ttggacaatc atcagataaa attgagacta tatcgagtat ataaatgttt gtatagtcaa
     4381 tttattcaaa atcgtcaagt tctaaattat ttaaaggact ataacattca tatttcttta
     4441 agtaattg
//
LOCUS       DAVLYV010000113         4179 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLYV010000113 DAVLYV010000000
VERSION     DAVLYV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.113"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            307..2808
                     /locus_tag="VYO70_001995"
     CDS             307..2808
                     /locus_tag="VYO70_001995"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1714150.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTS
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEIVAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFVQLIAPFAPHL
                     AEELWQTVAATGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKVEIDGKEIVKVIAVPNKLVNIVVK"
     gene            3003..3698
                     /locus_tag="VYO70_001996"
     CDS             3003..3698
                     /locus_tag="VYO70_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1714151.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQESVADMEELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPKLRGTRIGTEAQYLLARYVFEELNYRRYEWKCDSL
                     NLPSRRAAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKARLEAWLTPENF
                     DKTGQQYKSLREL"
     gene            3710..4126
                     /locus_tag="VYO70_001997"
     CDS             3710..4126
                     /locus_tag="VYO70_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1714152.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YVALDGDAVVGLIRLVGDGFSSVFVQDLIVLPNYQRQGIGSLLMKEALKDYKDAYQVQ
                     LVTEETEKNVRFYRSMGFEALSTYDCIGMTWVNREK"
BASE COUNT         1252 a          809 c          970 g         1148 t
ORIGIN      
        1 agattcagtc cactatacgt tcttcataaa tcaaaaagcg acataggttg tcggctattt
       61 attgtgaata cattaattag cattccagtt ttatcttcgg tctaaaataa gtattttgtg
      121 ctatacgaga taagcttctt gacttactcc ttgatttact gcataacaat gggataaaaa
      181 gtgggagata gagcaattca tagtcatcaa aattaatgag atacagtata cagttttttc
      241 tttaaacaca tttcaaattc cctcaaaaat ggtataatag taacatcaca aaattggaga
      301 gagaccatga gtttttacaa tcataaagaa attgaaccta agtggcaggg ctactgggca
      361 gaacatcata catttaagac aggaacagat gcatcaaaac ctaagtttta tgcgcttgat
      421 atgttccctt atccgtctgg agctggtctg catgtaggac acccagaagg ttatactgca
      481 accgatatcc tcagtcgtta caaacgtgcg caaggctaca atgtccttca cccaatgggt
      541 tgggatgctt ttggtttacc tgcagagcaa tacgctatgg atactggtaa tgacccagca
      601 gaatttacat cggaaaacat tgccaacttc aaacgtcaaa tcaatgcgct tggattttct
      661 tatgactggg atcgtgaagt caacacaaca gatccaaact actacaagtg gactcaatgg
      721 attttcacca agctttacga aaaaggcttg gcctatgaag ctgaagtgcc agtaaactgg
      781 gttgaggaat tgggaactgc cattgccaat gaagaagtgc ttcctgacgg aacttctgaa
      841 cgtggaggct atccagttgt ccgcaaacca atgcgccaat ggatgctcaa aatcacggct
      901 tacgcagagc gcttgctcaa tgacttagat gaactagatt ggtcagagtc tatcaaggat
      961 atgcaacgca actggattgg taaatcaact ggtgccaatg taactttcaa agtaaaagga
     1021 acagacaagg aatttacagt ctttactact cgtccggaca cacttttcgg tgcgactttc
     1081 actgttttgg ctcctgaaca tgaattagta gatgccatca caagcacaga gcaagcagaa
     1141 gcagtagcag actacaaaca ccaagccagc cttaagtcag acttggctcg taccgacctt
     1201 gccaaagaaa aaactggtgt atggactggt gcttatgcca tcaatcctgt caatggtaag
     1261 gaaattccaa tctggattgc ggattacgtt cttgctagct atggtacagg tgcggtaatg
     1321 gctgtgcctg cccacgatca acgtgactgg gaatttgcca aacaatttga ccttccaatc
     1381 gtcgaagtac ttgaaggtgg aaatgttgaa gaagctgcct acacagaaga tggccttcac
     1441 gttaattcag acttcctaga tggactcaac aaagaagacg ctattgccaa gattgtggct
     1501 tggttggaag aaaaaggctg tggacaagag aaggtaacct accgtctccg cgactggctc
     1561 tttagccgtc aacgttactg gggtgagcca atcccaatca ttcattggga agatggaact
     1621 tcaacagctg tccctgaaag tgaattgcca cttgtcttgc ctgtaaccaa ggatatccgt
     1681 ccctcaggta ctggtgaaag tccactagct aacttgacag actggcttga agtgactcgt
     1741 gaagatggtg tcaaaggtcg tcgtgaaaca aatactatgc cacaatgggc aggttcaagc
     1801 tggtactacc tccgctatat tgacccacac aatactgaga aattggctga cgaggatctg
     1861 ctcaaacaat ggttgccagt agatatctac gtgggtggtg cggaacatgc tgtacttcac
     1921 ttgctttacg ctcgtttctg gcataaattc ctctatgacc tcggtgttgt tccaactaag
     1981 gaaccattcc aaaaactctt taaccaagga atgattttgg gaacaagcta tcgtgaccac
     2041 cgtggggctc ttgtggcgac tgacaaggtt gaaaaacgtg atggttcttt cttccatgtg
     2101 gaaacagggg aagagttgga gcaagcgcca gccaagatgt ctaaatcgct caagaacgtt
     2161 gttaacccag acgatgtggt ggaacaatac ggtgccgaca ctcttcgtgt ttatgaaatg
     2221 tttatgggac cacttgatgc ttcgattgct tggtcagaag aaggtctaga aggaagccgt
     2281 aaattccttg accgtgttta ccgtttgatt acaagcaagg aaattgttgc ggaaaacaat
     2341 ggcgctctcg acaaagttta caacgaaacc gttaaagctg tcacagaaca aattgagtcc
     2401 ctcaaattca acacagccat tgctcaactt atggtctttg tcaacgctgc caacaaggaa
     2461 gacaaacttt atgtagacta cgccaaaggc ttcgttcagt tgattgctcc atttgctcct
     2521 cacttggcag aagagctctg gcaaacagtt gcagcaacag gagaatctat ctcttatgta
     2581 gcttggccaa cttgggatga aagcaaatta gttgaagacg aaatcgaaat cgtcgtccaa
     2641 atcaaaggta aagtccgtgc caaactcatg gttgctaaag acctgtcacg cgaagaattg
     2701 caagaaattg ctctcgctga tgaaaaagtc aaggtagaaa ttgacggtaa ggaaatcgtg
     2761 aaagtgattg cggtacctaa caaactcgtt aatatcgtcg ttaaataacg agtttattag
     2821 ctctatctgc caccttcaat agtccactgg actattgaag ccaactaaat tggttaatat
     2881 tgttgtgaaa taagatagga atccttcaga gtagaatctg ggggattttt tgaatcttct
     2941 tatgaaagta tgttatacta tggtcaacta taaaatttga aaagcgaaac aaggggaaag
     3001 ttatgccagt aaatgaatat ggtcagatga ttggtgagtc aatggaaggt tatacacctg
     3061 gtgcactgcc ttctattgat ttcttagaag ggcgttatgc tcgaatagaa gctctttcag
     3121 tggaaaagca tgcggaggat ttattagctg tttatggccc tgatacgcct cgggaaatgt
     3181 ggacctacct ctttcaggaa tcagtggcag atatggagga gctggttagc cttttaaatc
     3241 agatgttggc tcgtaaggac cgtttttact atgcaatcat agacaaggca actggtaagg
     3301 ctttgggaac tttttctctc atgcgtattg accagaataa tcgagtaata gaagtgggag
     3361 ctgtcacttt ttctccaaaa ctcaggggga cacggatagg gacagaagcc cagtatctac
     3421 tggcgcgcta tgtctttgaa gagcttaact atcgtcgcta tgagtggaaa tgtgattctc
     3481 ttaatctgcc atccagacga gcagcggaac gtttgggatt tgtctatgaa ggaaccttcc
     3541 gtcaggcagt ggtttataag gggcgtacga gggatacgga ttggttgtct atgattgata
     3601 aggactggcc tcaagtcaaa gctcgattgg aagcatggct gactcctgaa aattttgata
     3661 aaactgggca acaatacaag agtttgagag aactttaaga ggtgttgaga tgattactat
     3721 taaaaagcaa gaaattgtca agctagagga tgttttgcat ctctatcagg ctgtcggttg
     3781 gacaaattat acccatcaac cagagatgct ggagcaggcc ttatctcatt cattagtgat
     3841 ttatgtggcg cttgatggtg atgctgtagt gggcttgatt cgtttggttg gagatggttt
     3901 ttcatcagtt tttgtacagg atttgattgt tttgcctaac tatcagcgcc aagggattgg
     3961 tagtctcttg atgaaagagg ctttaaaaga ttacaaagat gcctatcaag tccagctggt
     4021 gacagaagag acagaaaaaa acgtaagatt ttatcgttct atgggttttg aagccttatc
     4081 cacctatgat tgtataggaa tgacttgggt gaatcgagaa aaataacaaa acttgttttt
     4141 tcttaagcaa agtttaagga tggtctagta tcatatagt
//
LOCUS       DAVLYV010000114         4118 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLYV010000114 DAVLYV010000000
VERSION     DAVLYV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4118
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.114"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            651..1643
                     /locus_tag="VYO70_001998"
     CDS             651..1643
                     /locus_tag="VYO70_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1714153.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1645..2463
                     /locus_tag="VYO70_001999"
     CDS             1645..2463
                     /locus_tag="VYO70_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1714154.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2465..3250
                     /locus_tag="VYO70_002000"
     CDS             2465..3250
                     /locus_tag="VYO70_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1714155.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3289..3567
                     /locus_tag="VYO70_002001"
     CDS             3289..3567
                     /locus_tag="VYO70_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1714156.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3552..3749
                     /locus_tag="VYO70_002002"
     CDS             3552..3749
                     /locus_tag="VYO70_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1714157.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1139 a          709 c          897 g         1373 t
ORIGIN      
        1 gaatatgaaa gctttttata tcactattga aaaatttata cgagatgatg aaagccttaa
       61 gtgttatttt ataaaggtta tttcaagtcg ttccaaggta acaagtctag atcagattga
      121 agctgataaa acgatacaaa gaaaatattc aagtgagcta aaaaaattta ttggatttta
      181 taatgagatt atttgtgagg aaaatagttt cctacatgta cgaaagaggt ggtcgagttg
      241 gtttaggtag tcgatgcgtg agttgataat tctcagggta tggacttctt tttcatgaat
      301 gaggtaaaag agcaggtatt gtttagagac aatcattctg agcatatttt ctggatagag
      361 ggagtatccg attttatgat caaagttaat accgccctct ggtgagaaga tgagtaggtt
      421 ggtaatttaa actattaaac agaatttttg attaaaagta ttattttatg agagaaatcc
      481 taatttcaca atccataggc aaactcttgc atttcgtttt ttattggact ataataggtt
      541 ggtataaagc cttttgtagt aataaaatgt agaaggtgta gaaagcaagg atttagaata
      601 tttgtagtta aaaacacaat gttgctattc cttacgatag ggagatagat atggcaatga
      661 tagaagtgga acatcttcag aaaaattttg tgaagactgt taaggaaccg ggcttgaagg
      721 gggctttgcg ctcctttatt catcctgaaa agcagacctt tgaagcggtc aaggatttga
      781 cctttgaggt tccaaaaggg cagattttag gatttatcgg ggcaaatggt gctgggaagt
      841 cgacaaccat taaaatgctg acaggaattt tgaaaccaac atctggtttt tgtcggatta
      901 acggcaagat tccccaggac aatcggcaag attatgtcaa agatattggc gtagtctttg
      961 gacaacgcac ccagctatgg tgggatttgg ctctgcaaga gacctacact gtcttaaaag
     1021 agatttatga tgtgccaaac tcgctctttc ataagcgcat ggactttttg aatgaagtct
     1081 tggatttgaa ggactttatc aaggatcccg tgcggactct ttcactggga caacggatgc
     1141 gggcggatat tgcggcctcc ttgctccaca atcccaaggt tcttttttta gatgagccga
     1201 ccattggttt ggacgtttcg gttaaggata atattcgtcg ggcaattact cagatcaatc
     1261 aagaggaaga aactaccatt cttttgacca ctcacgattt gagtgatatt gagcaacttt
     1321 gtgatcggat tttcatgatt gacaaggggc aagagatttt tgatggaacg gtgagccaac
     1381 tcaaggagac ctttggtaag atgaagactc tctcttttga actgctacca ggtcaaagtc
     1441 atctcgtctc tcactatgaa ggtctgtctg atatgaccat tgatagacaa ggaaacagcc
     1501 tcaacattga atttgatagt tctcgctacc agtcagctga cattatcaag caaaccctgt
     1561 ctgattttga aatccgcgat ttgaagatgg tggatacgga tattgaggat attatccgtc
     1621 gcttctaccg aaaggagctc taggatgatc aaattgtgga gacgttataa accctttatc
     1681 aatgcagggg ttcaggagtt gattacttac cgagtcaact ttattctcta tcggattggc
     1741 gatgtcatgg gggcttttgt ggccttttat ctctggaagg ctgtctttga ttcttcgcaa
     1801 gagtctttga ttcagggctt cagtatggcg gatatcaccc tctacatcat catgagtttt
     1861 gtgaccaatc ttctgactag atccgattcg tcctttatga ttggggagga ggtcaaggat
     1921 ggctccatta tcatgcgttt gttgcgacca gtgcattttg cggcctccta tcttttcacc
     1981 gagcttggtt ccaagtggtt gatttttatc agcgttggcc ttccattttt aagtgtcatt
     2041 gtcttgatga aaatcatatc gggtcaaggt attgtagagg tgctaggatt aactgtcctt
     2101 tatcttttta gcttaacgct cgcctatctg attaactttt tctttaatat ttgctttgga
     2161 ttttcagcct ttgtgtttaa aaatctttgg ggttccaacc tacttaagac ttccatagtg
     2221 gcttttatgt cggggagttt gattcccttg gcattctttc caaaggttgt ttcagatatt
     2281 ctttcctttt tgcctttttc atccttgatt tatactccag ttatgatcat tgttggaaaa
     2341 tacgatgcca gtcagattct tcaggcactc cttttgcagt tcttctggct cttagtgatg
     2401 gtgggattgt ctcagttgat ttggaaacgg gtccagtcct ttatcaccat tcaaggaggt
     2461 tagtatgaaa aaatatcaac gaatgcatct gatttttatc agacaataca tcaaacaaat
     2521 catggaatat aaggtggatt ttgtggttgg tgtcttggga gtctttctgg ctcaaggctt
     2581 gaatctcttg tttctcaatg tcatctttca acatattcca ttcctagaag gttggacctt
     2641 tcaagagata gctttcattt atggattttc cttgattccc aagggaatgg accatctctt
     2701 ttttgacaat ctctgggcac tagggcaacg cctagtccga aaaggagagt ttgacaagta
     2761 tctgactcgt cccatcaatc ctctctttca catcctagtt gaaacctttc agattgatgc
     2821 cttgggtgaa ctcttagtcg gtggtatttt attgggaaca acagtgacca gcattgtttg
     2881 gactcttccg aaattcctgc ttttcctagt ttgtattcct tttgcgacct tgatttatac
     2941 ttctcttaaa atcgcaacag ccagtatcgc cttttggact aagcagtcag gcgccatgat
     3001 ttacatcttc tatatgttca atgactttgc taagtatccg atttctattt acaattctct
     3061 tcttcgttgg ttgattagct ttatcgtgcc tttcgccttt acagcctact atccagctag
     3121 ctatttctta caggaaaagg atgtgttctt taacgtagga ggtttgatgt tgatttctct
     3181 ggttttcttt gttatttccc ttaaactttg ggataagggc ttagattcct acgaaagtgc
     3241 gggttcgtaa aagctaaagt aagactaaaa tcaagaaaga aacttatgat gtttgtaatt
     3301 gaagaagtca aggatgaaaa tcaaaaaaag gcagttgtag ctgaggtttt gaaggatttg
     3361 ccagaatggt ttggaatccc agaaagcaca caagcctata tagaaggaac cacgacactg
     3421 caagtttgga ccgcctatca ggagagtgat ttgactagat ttgtaagctt atcctattcg
     3481 agtgaagatt gtgcagagat tgattgtctc ggcgtaaaaa agcttatcaa ggtagaaaaa
     3541 ttgggagcca attgcttgct actttagaga gtgaagctcg taaaaaagtt ggttatctgc
     3601 aggtcaaaac agtggcagaa ggttctaata aagattatga tcgaacaaat gacttttatc
     3661 gaggtcttgg ctttaaaaag ttagagattt ttcctcaact atggaatccg caaaatcctt
     3721 gtcagatttt gattaaaaag cttgaataat attacttgac atctattctc agagtgccat
     3781 actgtaagtg taatcgccga tttagctcag ttggtagagc aaggcactcg taaagcctag
     3841 gttataggta gataaacgac tgaggagttg aaaaaataga taggtagaag ataaccgtta
     3901 agccttattc ttagcggtta tttatattgt ttaatagcgc tgatatttca tcaattatgc
     3961 ctgttttcgt gtttctggta gttgttcaag tttattgcta ctatttttga tggtatgaat
     4021 gtgcttataa tgtatcccgg ttaacgaaag ttttggactt atactcttag aaaatctctt
     4081 caaaccacgt caacgtcgcc ttgccgtgcg tatggtta
//
LOCUS       DAVLYV010000115         4091 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLYV010000115 DAVLYV010000000
VERSION     DAVLYV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.115"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            434..661
                     /locus_tag="VYO70_002003"
     CDS             434..661
                     /locus_tag="VYO70_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714158.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            949..3846
                     /gene="nanA"
                     /locus_tag="VYO70_002004"
     CDS             949..3846
                     /gene="nanA"
                     /locus_tag="VYO70_002004"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1714159.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDASAPRFYNLFSVSS
                     DTKEDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPKGRVRLYVNGVLSRTSLRSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGNPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1391 a          685 c          900 g         1115 t
ORIGIN      
        1 gtgttttgag ctgacttcgt cagttctatc tgccacctca aaacggtgtt ttgagctgac
       61 tttgtcagtc ttatctacaa cctcaaaaca gtgttttgag catcatgcgg ctagcttctt
      121 agtttgctct ttgattttca ttgagtataa aaacagatga gtttctgttt tctttttatg
      181 gactataaat gttcagctga aactactttc aaggacatta ttatataaaa gaattttttg
      241 aaactaaaat ctactatatt acactatatt gaaagcgttt taaaaataag gtataataaa
      301 tttactaaca cttataaaaa gtgatagaat ctatctttat gtatatttaa agatagattg
      361 ctgtaaaaat agtagtagct atgcgaaata acagatagag agaagggatt gaagcttaga
      421 aaaggggaat aatatgatat ttaaggcatt caagacaaaa aagcagagaa aaagacaagt
      481 tgaactactt ttgacagttt ttttcgacag ttttctgatt gatttatttc ttcacttatt
      541 tgggattgtc ccctttaagc tggataagat tctgattgtg agcttgatta tatttcccat
      601 tatttctaca agtatttatg cttatgaaaa gctatttgaa aaagtgttcg ataaggattg
      661 agcaggaagt atggtgtaaa tagcataggc tgatgtccat catttgctta taaagagata
      721 ttttagttta attgcagcgg tgccctggta gataaactag attggcagga gtctgattgg
      781 agaaaggaga ggggaaaatt ggcaccaatt tgagatagtt tgtttagttc atttttgtca
      841 tttaaatgaa ctgtagtaaa agaaagttaa taaaagacaa actaagtgca ttttctggag
      901 taaatgtctt atttcagaaa tcgggatata gatatagaga ggatcagtat gaatcggagt
      961 gttcaagaac gtaagtgtcg ttatagcatt aggaaactat cggtaggagc ggtttctatg
     1021 attgtaggag cagtggtatt tggaacgtct cctgttttag ctcaagaagg ggcaagtgag
     1081 caacctctgg caaatgaaac tcaactttcg ggggagagct caaccctaac tgatacagaa
     1141 aagagccagc cttcttcaga gactgaactt tctggcaata agcaagaaca agaaaggaaa
     1201 gataagcaag aagaaaaaat tccaagagat tactatgcac gagatttgga aaatgtcgaa
     1261 acagtgatag aaaaagaaga tgttgaaacc aatgcttcaa atggtcagag agttgattta
     1321 tcaagtgaac tagataaact aaagaaactt gaaaacgcaa cagttcacat ggagtttaag
     1381 ccagatgcat cagctccacg cttttacaat ctcttctctg tatccagtga caccaaggaa
     1441 gatgagtact tcactatggc agtttacaat aatactgcta ctctagaggg gcgtggttcg
     1501 gatgggaaac agttttacaa taattacaac gatgcaccct taaaagttaa accaggtcag
     1561 tggaattctg tgactttcac agttgaaaaa ccgacagcag aactacctaa aggccgagtg
     1621 cgcctctacg taaacggggt attatctcga acaagtctga gatctggcaa tttcattaaa
     1681 gatatgccag atgtaacgca tgtgcaaatc ggagcaacca agcgtgccaa caatacggtt
     1741 tgggggtcaa atctacagat tcggaatctc actgtgtata atcgtgcttt aacaccagaa
     1801 gaggtacaaa aacgtagtca actttttaaa cgctcagatt tagaaaaaaa actacctgaa
     1861 ggagcggctt taacagagaa aacggacata ttcgaaagcg ggcgtaacgg taacccaaat
     1921 aaagatggaa tcaagagtta tcgtattcca gcacttctca agacagataa aggaactttg
     1981 atcgcaggtg cagatgaacg ccgtctccat tcgagtgact ggggtgatat cggtatggtc
     2041 atcagacgta gtgaagataa tggtaaaact tggggtgacc gagtaaccat taccaactta
     2101 cgtgacaatc caaaagcttc tgacccatcg atcggttcac cagtgaatat cgatatggtg
     2161 ttggttcaag atcctgaaac caaacgaatc ttttctatct atgacatgtt cccagaaggg
     2221 aagggaatct ttggaatgtc ttcacaaaaa gaagaagcct acaaaaaaat cgatggaaaa
     2281 acctatcaaa tcctctatcg tgaaggagaa aagggagctt ataccattcg agaaaatggt
     2341 actgtctata caccagatgg taaggcgaca gactatcgcg ttgttgtaga tcctgttaaa
     2401 ccagcctata gcgacaaggg tgatctatac aagggtgacc aattactagg caatatctac
     2461 ttcacaacaa acaaaacttc tccatttaga attgccaagg atagctatct atggatgtcc
     2521 tacagtgatg acgacgggaa gacatggtca gcgcctcaag atattactcc gatggtcaaa
     2581 gccgattgga tgaaattctt gggtgtaggt cctggaacag gaattgtact tcggaatggg
     2641 cctcacaagg gacggatttt gataccggtt tatacgacta ataatgtatc tcacttaaat
     2701 ggctcgcaat cttctcgtgt catctattca gatgatcatg gaaaaacttg gcatgctgga
     2761 gaagcggtca acgataaccg tcaggtagac ggtcaaaaga tccactcttc tacgatgaac
     2821 aataaacgtg cgcaaaatac agaatcaacg gtggtacaac taaacaatgg agatgttaaa
     2881 ctctttatgc gtggtttgac tggagatctt caggttgcta caagtaaaga cggaggagtg
     2941 acttgggaga aggatatcaa acgttatcca caggttaaag atgtctatgt tcaaatgtct
     3001 gctatccata cgatgcacga aggaaaagaa tacatcatcc tcagtaatgc aggtggaccg
     3061 aaacgtgaaa atgggatggt ccacttagca cgtgtcgaag aaaatggtga gttgacttgg
     3121 ctcaaacaca atccaattca aaaaggagag tttgcctata attcgctcca agaattagga
     3181 aatggggagt atggcatctt gtatgaacat actgaaaaag gacaaaatgc ctatacccta
     3241 tcatttagaa aatttaattg ggaatttttg agcaaagatc tgatttctcc taccgaagcg
     3301 aaagtgaagc gaactagaga gatgggcaaa ggagagatgg gcaaaggagt tattggcttg
     3361 gagtttgact cagaagtatt ggtcaacaag gctccaaccc ttcaattggc aaatggtaaa
     3421 acagcgactt tcctaaccca gtatgatagc aaaaccttgt tgtttgcagt agataaggaa
     3481 gatatcggac aggaaattat tggtatagct aaaggaagca tcgaaagtat gcataatctt
     3541 cctgtaaatc tagcaggtgc cagagttcct ggcggagtaa atggtagcaa agcagcggtg
     3601 catgaagttc cagaatttac agggggagtt aatggtacag agccagctgt tcatgaaatc
     3661 gcagagtata agggatctga ttcgcttgta actcttacta caaaagaaga ttatacttac
     3721 aaagctcctc ttgctcagca ggcacttcct gaaacaggaa acaaggagag tgacctccta
     3781 gcttcactag gactaacagc tttcttcctt ggtctgttta cgctagggaa aaagagagaa
     3841 caataagaga agaattctaa acatttgatt ttgtaaaaat agaaggagat agcaggtttt
     3901 caagcctgct atcttttttt gatgacattc aagctgatac gaaatcataa gaggtctgaa
     3961 actactttca gagtagtctg ttctataaaa tatagtagat tgaaataaga tgtgaacaac
     4021 tctatcagga aagtcaaatt aatttataga aaaattttag cagtcaaggt gtactgttat
     4081 agattcaata t
//
LOCUS       DAVLYV010000116         4071 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLYV010000116 DAVLYV010000000
VERSION     DAVLYV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.116"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            220..1665
                     /gene="cps4A"
                     /locus_tag="VYO70_002005"
     CDS             220..1665
                     /gene="cps4A"
                     /locus_tag="VYO70_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1714160.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSLSVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKLSQNTDLT
                     VDQSSSYLVAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALSFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMIDLVNAQLESGGTYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1667..2398
                     /locus_tag="VYO70_002006"
     CDS             1667..2398
                     /locus_tag="VYO70_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV1714161.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVASDLVIAYGAEIYYTPDVLDKLGKKRIPTLNDS
                     RYALIEFSMNTLYRDIHSALNKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRVQYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            2407..3099
                     /gene="cpsC"
                     /locus_tag="VYO70_002007"
     CDS             2407..3099
                     /gene="cpsC"
                     /locus_tag="VYO70_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1714162.1"
                     /translation="MQDQNTLEIDVFQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGSYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPATSPSSPNIKRNTLIGFLAGGIGTSVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            3109..3792
                     /gene="cps4D"
                     /locus_tag="VYO70_002008"
     CDS             3109..3792
                     /gene="cps4D"
                     /locus_tag="VYO70_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4D"
                     /protein_id="HEV1714163.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVISGVFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVIQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITRKCDASILVTATGEANKRDVQKVKQQLEQTGKLFLGVVLNKLDISVDKYGVY
                     GSYGNYGKK"
BASE COUNT         1294 a          664 c          845 g         1268 t
ORIGIN      
        1 ttagattgaa actagagtag tagacctctg cttctaaaac attgttagaa atcgatttga
       61 ctgtcctgat cgatttgtca tgttcttatt tcattttact atatttttgt ttcgcgggaa
      121 gtctactaag atacttaaag atgcagatag taaaaaaatg tagacattac cgtaaaaaag
      181 tgatataatc ataagatgtt caatgtatag gtgttaatca tgagtagacg ttttaaaaaa
      241 tcaggttcac agaaagtgaa gcgaagtgtt aatatagttt tgctgactat ttatttattg
      301 ttagtttgtt ttttattgtt cttaatcttt aagtacaata tccttgcttt tagatatctt
      361 aatctagtgg taactgcgtt agtcttacta gtagccttgg tagggctact cttgattatc
      421 tataaaaaag ctgaaaaatt tactattttt ctgttggtgt tctctatcct tgtcagctca
      481 gtgtcgctct ttgcagtaca gcagtttgtt ggactgacca atcgtttaaa tgcgacttct
      541 aattactcag aatattcgct cagtgtcgct gttttagcag atagtgagat cgaaaatgtt
      601 acgcaactga cgagtgtgac agcaccgact gggactgata atgaaaatat tcaaaaacta
      661 ctagctgata tcaagttaag tcagaatacc gatttgacag tcgaccagag ttcgtcttac
      721 ttggttgctt acaagagttt gattgcaggg gagactaagg ccattgtcct aaatagtgtc
      781 tttgaaaata tcatcgagtc agagtatcca gactacgcat cgaagataaa gaagatttat
      841 accaagggat ttactaaaaa agtagaagct cctaagacgt ctaagaatca gtctttcaat
      901 atctatgtta gtggaattga cacctatggt cctattagtt cggtgtcgcg atcagatgtc
      961 aatatcctga tgactgtcaa tcgtgatacc aagaaaatcc tcttgaccac aacgccacgt
     1021 gatgcctatg taccaatcgc agatggtgga aataatcaaa aagataaatt aacccatgcg
     1081 ggcatttatg gagttgattc gtccattcac accttagaaa atctctatgg agtggatatc
     1141 aattactatg tgcgattgaa cttcacttct ttcttgaaaa tgattgactt attgggaggg
     1201 gtagatgttc ataatgatca agagttttca gctctacatg ggaagttcca tttcccagta
     1261 gggaatgtcc acctagactc tgagcaggct ctaagttttg tacgtgaacg ctactcacta
     1321 gccgatggag accgtgaccg tggtcgcaac caacaaaagg tgattgtggc tatccttcaa
     1381 aaattaacgt caaccgaagc actgaaaaat tatagtacga tcattgatag cttgcaagat
     1441 tctatccaaa caaatatgcc acttgagacc atgatagatt tggtcaatgc tcagttagaa
     1501 agtggtggaa cttacaaagt gaattcgcaa gacttgaaag gtacaggtcg gatggatctt
     1561 ccttcttatg caatgccaga cagtaacctc tatgtgatgg aaatagatga tagtagttta
     1621 gctgtagtta aagcagctat acaggatgtg atggagggta gatgaaatga tagacatcca
     1681 ttcgcatatc gtttttgatg tagatgacgg tcccaagtca agagaggaaa gcaaggctct
     1741 cttggcagaa tcctacaggc agggggtgcg aaccattgtc tctacctctc accgtcgcaa
     1801 gggcatgttt gaaactccgg aagagaagat agcagaaaac tttcttcagg ttcgggaaat
     1861 agctaaggaa gtggcgagtg acttagtcat tgcttacggg gctgaaattt actacacacc
     1921 agatgttctg gataagctgg gaaaaaagcg gattccgacc ctcaatgata gtcgttatgc
     1981 cttgatagag tttagtatga acactcttta tcgcgatatt catagcgcct tgaacaagat
     2041 cttgatgttg ggaattactc cagtcattgc ccacatcgaa cgctatgatg ctcttgaaaa
     2101 taatgaaaaa cgcgttcggg agctgatcga tatgggctgt tacacgcaag taaatagttc
     2161 acatgtcctc aaacccaaac tttttggcga acgttataaa ttcatgaaaa aaagagttca
     2221 gtattttttg gaacgtgatt tagttcatgt agttgcaagt gatatgcaca atttagacag
     2281 tagacctcca tatatgcaac aggcatatga tatcattgct aagaaatatg gagcgaaaaa
     2341 agcgaaagaa ctttttgtag ataatcccag aaaaattata atggatcaat taatttagga
     2401 gaaaatatgc aagatcaaaa cactttggaa atcgatgtat ttcaactatt cagagcttta
     2461 tggaaaagaa agttggtcat tttattagtg gcaattataa cttcttcagt tgcttttgcc
     2521 tacagtactt ttgttatcaa acctgagttt actagtacga cccggattta tgtagttaac
     2581 cgtaatcagg aagagaaatc tggtttaacc aatcaagact tgcaggcagg ttcatacttg
     2641 gttaaagact atcgtgaaat tatcctatcg caggatgttt tggaggaagt tgtttctgat
     2701 ttgaaactag atttgacgcc aaaaggtttg gctaataaaa ttaaagtaac agtaccagtt
     2761 gatacccgta ttgtatctgt ttcagttaat gatcgagttc ctgaagaggc aagccgtatc
     2821 gctaactctt tgagagaagt agctgctcaa aaaattatca gtattactcg tgtttctgat
     2881 gtgacaacac tggaggaggc aagaccggcg acatcaccgt cttcgccaaa tattaaacgc
     2941 aatacactaa ttggtttttt ggcagggggg attggaacta gtgttatagt tcttcttctt
     3001 gaacttttgg acactcatgt gaaacgtccg gaagatatcg aagatacact gcagatgaca
     3061 cttttgggag ttgtaccaaa cttgagtaag ttgaaatagg agagaggaat gccgacatta
     3121 gaaatagcac aaaaaaaact ggagttcatt aagaaggcag aagaatatta caatgccttg
     3181 tgtacaaata tacagttgag cggagataaa ctaaaagtaa tttccgttac ttctgttaac
     3241 cctggggaag gaaaaacaac tacttccgta aatatagcaa ggtcgtttgc gcgtgcaggc
     3301 tataaaactc ttttgatcga tggcgatact cgaaattcag ttatatcagg agtttttaaa
     3361 tcgcgtgaaa aaattacagg gctaacagaa tttttatctg ggacagctga tttatctcac
     3421 ggtttatgtg atacaaatat tgaaaattta tttgtaattc aatcgggatc tgtatcacca
     3481 aaccctacgg ccttgttaca aagtaaaaat tttaatgata tgattgaaac attgcgtaaa
     3541 tattttgatt atatcattgt tgatacacca cctattggaa ttgttattga tgcggcaatt
     3601 atcactcgaa agtgtgatgc gtccatcttg gtaacagcaa caggtgaggc gaataaacgt
     3661 gatgtccaaa aagtgaaaca acaattagaa caaacaggga aactgttcct gggagttgtt
     3721 ttaaataaat tggatatctc ggttgataag tatggagttt acggttccta tggaaattat
     3781 ggtaaaaaat aacttagaaa agattttatg gatgaaaaag gattgaaaat ttttctggca
     3841 gtattacaga gtattattgt cattttattg gtttattttc ttagctttgt tagagagaca
     3901 gaagttgaac gttcttcgat ggttatacta taccttctcc acttttttgt attctatttt
     3961 agttcctatg gtaacaattt ttttaaaaga gggcacctag ttgagtttaa tagtactata
     4021 agatatattt ttttctttgc aatagctata agtgtattaa acttttttat a
//
LOCUS       DAVLYV010000117         4053 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLYV010000117 DAVLYV010000000
VERSION     DAVLYV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.117"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(116..391)
                     /locus_tag="VYO70_002009"
     CDS             complement(116..391)
                     /locus_tag="VYO70_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1714164.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(498..1337)
                     /locus_tag="VYO70_002010"
     CDS             complement(498..1337)
                     /locus_tag="VYO70_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1714165.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1479..2327
                     /locus_tag="VYO70_002011"
     CDS             1479..2327
                     /locus_tag="VYO70_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1714166.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2420..3337)
                     /locus_tag="VYO70_002012"
     CDS             complement(2420..3337)
                     /locus_tag="VYO70_002012"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1714167.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVEQLKADEEVTRNWS"
     gene            3775..3980
                     /locus_tag="VYO70_002013"
                     /pseudo
     CDS             3775..3980
                     /locus_tag="VYO70_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1259 a          817 c          652 g         1325 t
ORIGIN      
        1 atacaatatt ttcggaacat tcaacttttt aactctattt attactagat ttcataatta
       61 aaaagcctac tgaccaagct agaaagcttg atacaatagg ctttttaaag actgattatt
      121 taacagcgtc tttaagagct ttaccagctt tgaatgctgg tactttagaa gctgcaattg
      181 tcatttcttt accagtttgt gggttgcgac ctttacgttc tgcacgctca cgaacttcaa
      241 agttaccaaa accgatcaac tgaacttttt caccagctgc aagatagtca gctactgctg
      301 caaatacagc ttcaactgct gctgctgagt ctttcttagt caattctgta gcttctgcta
      361 ctttagcgat caaatcttgt ttgtttgcca tgttaatgat tcctccaaat aatttctaat
      421 taacaaatat aatcatatcc taaaatccct tttaggtcaa gttaaaaacg ctatttcttc
      481 ccattttatt tattttttta ggagtggtaa cgtatcaaaa tagcccaagc gttctcaccc
      541 gtgtgagttt gaataatgga acccgtttcc aaaacagaaa ttggcttttc aacataagct
      601 tgtaagcttt ctttcatctc ttttgcccaa tcatcactac cagaatatga aattccaatc
      661 tctgctacag cacgttcaga aagcgatgtt atcaactcat ctaaccattt tttaaatgtt
      721 ttagttccac gacctttaac cattggctgc aattcatggt ctttcatttg catgacaaca
      781 cggatattga gaagagagct caacaagcca gttacacggc taattcgtcc acctttgaca
      841 agattttcca aagttgaaac accaatataa agctctgtat ggtttttaac ctcttctaca
      901 tgagataaaa ttgcctccat atctttacct tcttgagcta acttcgcagc ctcaacaact
      961 tggaatttca gggcttggtc agtgaaggaa ctatcaacaa cagtcacatc tgcagtagat
     1021 aggctagcac cttggcgtgc tgcttctacc gtacccgaaa gagcatggga catatgaata
     1081 gcaagaatct ggccaccatc tttgcatagg tcttcaaaaa tctcagcaaa gacacctaca
     1141 gggggctgac ttgttttcgg aagattctta ctttcttgca tcaactgaag aaatttacct
     1201 tcttctttca aatccgcatc agaataaaca acattatcaa tcattacaga taatggaaca
     1261 attgtaatat ctaattgctt tactagttca ggttcaatag taacagatga atcggttaca
     1321 atcttaattt ttgtcatagt atcaatcttt ctattttagg attcagattg gtttccttac
     1381 ttctaattat atcaaaaaaa agattaaaaa tcctaatgga gtcaatcaaa ttttccgtaa
     1441 aatttgatat aatcaactta taagaaaaga ggtgtcctat gattaaaaaa atttacccca
     1501 tttttaccat tttactaggt gctgctattt atgcttttgg actgacttat tttgtagttc
     1561 cccatcatct ctttgaagga ggggcgacag gcattaccct catcaccttt tatcttttta
     1621 aaatccctgt ttccctcatg aacctgctga ttaatattcc ccttttcatc ctagcttgga
     1681 agatttttgg agccaaatcc ctctattcta gtttactagg aaccttagct ttgtccggct
     1741 ggttagcttt ttttgagcat attccccttc atattgatct tcaaggtgat ttactaatca
     1801 cagcccttat agcgggaatc ctattgggaa ttggccttgg aattattttt aatgctggag
     1861 gtacaactgg cggaactgat attctagctc gtattctcaa caaatacact catatatcca
     1921 taggaaaact gctctttatc ttagattttt gtattctcat gttgattctc ctaatcttca
     1981 aggatttgag attggtttcc tacacgcttt tgtttgattt tattgtttct cgtgttattg
     2041 atttgattgg tgaaggagga tatgccggca aaggctttat gattatcaca aaacgtcctg
     2101 accaacttgc taaggcgatt aatgatgacc tcggaagagg tgttactttt atttctggtc
     2161 aaggctacta tagtaaagaa aatttgaaaa tcatctactg tattgtcgga agaaatgaaa
     2221 ttgtgaaaac gaaggaaatg attcatcgaa tcgatcctca agcctttata actattacag
     2281 aagcccatga aatcctagga gaaggcttca cctttgaaaa agaataaaaa gaggtagtgt
     2341 cgtgacctca aaagttagac taaatcatct atcttttggg ttacagacaa cctctttttt
     2401 gttttattta ctcaagctct taagaccaat tccgagttac ttcttcatca gcctttaact
     2461 gttccactaa ttggtcaact gagtcaaatt tggtcatatc acgaatgcga tcaagccaat
     2521 aaaccatgac ggtttcccca taaatatctt gattaaaatc aaaaatattg acttcaaaac
     2581 gtgcttcttc tccatcaaag gtcacatttt tcccgacact agccatagca cgatacttct
     2641 gtctttgaat ctcaacatca acgacataaa cgccatctgc tggcatataa gtacggtcta
     2701 aaagcactaa attcgctgtc ggataaccaa ttgtacgacc acgagcatta ccatgaacca
     2761 ccatacctct tgatggaagc ggtgccccca aaagttttcc tgcttctttc acatttccat
     2821 ctaaaatagc ttgacggata cgagttgaac taatctttcc tttctcatct tctacaggtg
     2881 gaacaatgat aacttctcca tcaaagtaat cctttaaatc ttctgctgtt tttttgtcag
     2941 aaccaaatgt ataatcaaaa cctgcaacaa taattttggc attcatagcc ttgatataag
     3001 ttgcaaagaa ttcttgtgca gtgagactag cgaattgact actaaaatca aggagatata
     3061 attcttctac accttcgcgc tttaattttc tttcacgttc agcagggttc aaaatatgca
     3121 aaaacaaatc tggatgataa ggctctaaag cgatctttgg agattcatta aaggtcataa
     3181 cgacgatagg taataaatcc tttctcgcag ccttgttggc aacacgaaat aattcttgat
     3241 gccccttatg tatgccatca aaatagccga gaacaacgac tgaatcagat ggtgtgccaa
     3301 tatctttttg gttttttata ggaatagtaa taatcataaa ataattatat catagcgata
     3361 gctatttctg gaacagaaaa tctgaaatgt tgtttttttc acatgaagtg tacctgtttt
     3421 caaaaagcac tttattctat cgttgcttaa ctatgaactt tgcaatattc ttctcaaaaa
     3481 cttgtaggac atcttcaaaa ttttgcaagg agtgattaga cttgttcggt aaccataaag
     3541 tgtcatacta tgcttatgta tgaaaaagca atgcaactaa ctcctgagaa ctttaaatta
     3601 ctaattggtg ccgaaaaggt agaatttaga atcgaggtac acctatggct gtaaaattta
     3661 caaaatgaga caacttgggc aagatgtttg aagaatttcc taaactccct gatttgaagc
     3721 aagtcacttt ccctgatgac aaagaaaaaa gccaaaacag caaagaaaaa ctagatgact
     3781 gctttccaac aactcccatc tagtgtgctt cagactaggc tatttttctc ttcatctgtt
     3841 agcttggatt ctcagaccgt ttcagctaaa gaatatcttt tcccttatca gaaggaacgg
     3901 ctcaaaccat tcagacaagt gaagggacga caagccaata tttgaaacca gatagcagtt
     3961 cttatagtca attgaaataa aatctgaaga aatcgagtag gaaactcata tcaattttta
     4021 acagtgttct attccagatt catactcaat gaa
//
LOCUS       DAVLYV010000118         4050 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLYV010000118 DAVLYV010000000
VERSION     DAVLYV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.118"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(153..1568)
                     /locus_tag="VYO70_002014"
     CDS             complement(153..1568)
                     /locus_tag="VYO70_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1714168.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1581..3419)
                     /locus_tag="VYO70_002015"
     CDS             complement(1581..3419)
                     /locus_tag="VYO70_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1714169.1"
                     /translation="MSYKDKVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     MKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3437..>4050)
                     /locus_tag="VYO70_002016"
     CDS             complement(3437..>4050)
                     /locus_tag="VYO70_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1714170.1"
                     /translation="IINLGKITLGNNFDEIIYINLTDHISSSIERYKEGIIISNPLRW
                     EISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHFVNANLENNFQESYKITEI
                     IMGIEKIIQEFYYTEFNQDSIDYYRFITHIKLFAHRLVENTTYCDDDDEDLLALMKNK
                     YPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1356 a          796 c          533 g         1365 t
ORIGIN      
        1 tatttataat cggctctctg taacaaaatt aaattaatta atccccctat aaaataaaga
       61 ggtttagaat ttgatgtctt ccaaacctct ttatttcata tttaatgaga tgttgcctta
      121 accgtgataa tatctaatca tcaataaaca aactatttga ataaggattc tccatttgat
      181 tcaatcactc ctttatacca agtaaaggac attttcttat atcgattgaa tgtgccattt
      241 ccatcatcat ttcgatcaac ataaatgaga ccgtaccttt tagaaagttg tgcagtggac
      301 atagaaacgc agtcaataca tccccaagat gtatagccca taatttcaac accatcctgt
      361 agagcttcag caacttgcaa taaatgttct ttcatatact gaattctata atcatcttgg
      421 acggttaagt tattaagttc atcttttatt agttgatctt tagcacctaa tccattttct
      481 actataaata atgggatttg ataacggtca taatatctat ttaaaattat acgtagtcca
      541 attggatcaa tttgccatcc ccactctgaa gactctaaat aaggatttac taaaccacca
      601 ataatattcc cttctcctga attatactgt gttggaagag cagattgagt cacactcatg
      661 taatagctaa aggataaaaa atctacggta taatttttta ataactctgc atcttcagct
      721 gcaaactcta tgttaatgtc atgttcctta aaatatcttt ttgcataatt cggataataa
      781 cctctaacat gcacatctga aaatagataa tttagattct catactcatg agtcgcccat
      841 acatcttttg gatttggagt cattggataa gctggcatag ctaataccat acaccccacc
      901 ttaaactctg aattaatctc acgagcaatt tttgtaacca aacttgaggc gactaattca
      961 tgatgtatag cttgatataa ttcttgtttc gaaagattct ccttaggtat atctattcct
     1021 ccactagtaa atggtaattc caaaacagag tttacttcgt taaatgtaag ccaatattta
     1081 actttatctt tatacctttc taaaactgtt cgagcaaatt tttcataaaa atgaatcatt
     1141 ctcctatcaa cccatccatg atattttctt gctaaatata atggagtctc atagtgtgaa
     1201 agagttacaa gtggctctat cccgtgagca tgtagttcat caaacaattc atcataatat
     1261 ttcaaaccag cttcgttagg ttcttcttca tctccttttg gaaaaattct actccatgca
     1321 atagaagtac gaaaaacatt aaagcccatt tcagaaaaca aggatatatc ttccttatat
     1381 ttatgataaa aatcaatacc tatcaatttt aagttatctt ctgtaggatt ttctgttgct
     1441 tctcctaatc cacctttggg taacacatcc tgaactgata agcccttacc atcttcatta
     1501 tatgctccct ctacttgatt agctgcaaca gctccacccc aaagaaaatc atctggaaaa
     1561 atggtcataa ctttcctcca ttataatatt accagtaatt ccttagaatg ctcgattgtc
     1621 tgattattag gtaatactaa tacatctaga aaatcattgg tattcgttac aattactggt
     1681 gtaactgttt cgtagccttt agtcttgatt aaattcaagt ccatttcaaa aatcaactga
     1741 tttttgaaaa ctctgtctcc ttcttctaca tgactaataa aaccttgacc ttttagctca
     1801 acagtatcta atccaatatg aattagtaac tcaacaccct catcactctt caatccaatt
     1861 gcgtgcttag tcggaaaaat atttgtaatt ttcccatcaa atggtgcata aaccttacct
     1921 tcacttggga taatcgctac tccgtctcca attagtttat ctgaaaatgt tttatcctgg
     1981 acatcgctta acggaatgat ttctcctgat ataggagaaa atatcatttt tttatttgaa
     2041 actccagctt taacttctaa attactagaa ctctcttctt catcgattcc aaatatataa
     2101 gctaatacaa aggtaataac aaccgaaatg accgccacaa ttaaagcatt tacaatattt
     2161 gatggtacat cagaataaat aaattgaggc aacgctatca aagatgggac agcaaataga
     2221 tacgctttaa cactagtaag acctgcaaat aatcccgcta atccaccacc aatcatagct
     2281 gcataaagcg gttttttata ttttaaagtc acaccatata atgcaggttc ggtaattcct
     2341 gcaagtaagg ctgagaaacc tgctgcaaaa gcaatttgtt ttgtattatt atttttactc
     2401 tttaatgcaa cagccatcga agcagcccct tgagctaagt ttgaccctaa cattgctgga
     2461 agaattaata cgtctggagt agcaatagat gccgccaaaa aaataggtgc aaaagcccaa
     2521 tgcattccag tcataacaat aaatggcata atagcaccaa gaatagctaa tgtaagccat
     2581 ccagctacac catacatttg cccaactaga tttgataatc cttcaccaac aattactcca
     2641 ataggtccga ctacaactaa ggcaatacag cttgatacta ataatactag cgtaggttgc
     2701 aaaaaactct tagtaacagc tggtgttaat ttagcaatca ttttttcaat atatttcatc
     2761 aaccaaacca taataagaat tggaacgact gacgaaccat aactagctgg tgtcacaggt
     2821 acaccaaata aactaagagg attccctgat tgcaccattt gaacaaaatt tggatggaga
     2881 agtacacctg ctacagacat agctaatgta gatgttactt ttaatttttg tgatgcagaa
     2941 taagctaata acagcggtaa gaaataatat ggcgcatccc caaaaaatgt caaaaaagca
     3001 atagtctgag aatctgattg caatatacca agcattggta aaataattac caagactttc
     3061 agcatacctc cccctaacat tgctggaatg attggagtca tggaaccagc gatatactca
     3121 atgattcttt ctaaaatatt ccctttgtgc ccttgaacaa ctgaatcgga ttcaaaattg
     3181 ccaagtttaa cgaattcttt ataataatta gctacatcat taccaagtat aatttgatat
     3241 tgtccattct ttttcataat acctattaca cctggtatct tcttcatatc atcatcattg
     3301 actaaatttt catcttttaa ttctaatctt aaacgtgtta cacaatgggt aactctattg
     3361 acattttttt cacctccaat tacatcgagg attttttgta ccttatcttt ataactcatg
     3421 gtattctcct attctattaa tctaaatttt ttgttaagcg acgaatatga gccatcaaat
     3481 aaactaattc actagaagtc agcaaataat tgtactcagt ttgtataaac attgccacct
     3541 gttcaccaca ttcatattct ctaggatatt tatttttcat taatgctaac aagtcttcat
     3601 catcatcgtc acaataagtt gtattttcaa ccaagcgatg ggcaaataat tttatatgag
     3661 ttatgaatct ataataatca atagaatctt ggttaaactc agtataatag aactcttgaa
     3721 tgattttctc aattcccata attatttcag tgattttata cgactcttga aaattatttt
     3781 ctaaattagc attaacaaaa tgtagcgcta tgaatgcagc ttcgtcaatt ggaagttcaa
     3841 tacctaactc tttttttatt atttgtaaag cccttttccc aagttcaaat tcttctttat
     3901 aatatttcga tatttcccag cgtaggggat tcgaaataat aatcccttct ttataacgtt
     3961 ctatgctcga agaaatatga tccgttaaat taatatagat aatttcatca aaattattac
     4021 ccaatgttat tttacctagg ttaattattt
//
LOCUS       DAVLYV010000119         3782 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLYV010000119 DAVLYV010000000
VERSION     DAVLYV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.119"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(177..1727)
                     /locus_tag="VYO70_002017"
     CDS             complement(177..1727)
                     /locus_tag="VYO70_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1714171.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMSDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQILGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1743..2522)
                     /locus_tag="VYO70_002018"
     CDS             complement(1743..2522)
                     /locus_tag="VYO70_002018"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1714172.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2509..3360)
                     /gene="ylqF"
                     /locus_tag="VYO70_002019"
     CDS             complement(2509..3360)
                     /gene="ylqF"
                     /locus_tag="VYO70_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1714173.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
BASE COUNT         1108 a          801 c          710 g         1163 t
ORIGIN      
        1 ccagctttat gctttatcat ttctaactta attatagtct tatttttaat aaagttcaat
       61 tatttatatg taaaattcct catcagtaaa aaataaccga tctcatttct gagaatcggt
      121 tttctaaata aatcaaactg atcatttaca taacataaat tatgtgaatg atcatactac
      181 aacaacaaat ctttgacttt tccaatttca ctttttggaa taaccaggtg aatcatatca
      241 cccagataca ttctggttga gccgttaact gtttggctct tgccattatg gacttgagtt
      301 gtgatgagga cgttgtgtgg taagttgagt tcatgaactt gtttcccagc aattttatca
      361 gaaactggta tttcgataag tgtaacttct ccttcgctag atacttcttc tggaagcatt
      421 ttttccagca tggcttcata gactggcgta cctttgagca aatccatgat aatataagaa
      481 acaagagtga caagaccaag tggcatgagg ttgcgaatat ctcctaccat ctcagttacg
      541 aggatcatag cggttaaggg agcttttgat atggctccaa aatagccact cattcctaga
      601 atgacaaata tagggaattg ctcttgactg acaagtccaa gattgacaca gataacacca
      661 actaaggcac caagcaaaga accaagagct aaaatgggta ggaaaattcc tcctggcagt
      721 ccacttccat agctaatcat actccaaata aagcggatca aaaagtaagc taataaaact
      781 tggaaactaa aattttgttc agttaaagaa aggacaagct gatttccgcc accaaggatt
      841 tgaggtaaga agattccgac tggtatgata aggataaaag ccaagatggg ataataagcc
      901 ctatccaaat ggattttttg accaatcaag tcataaactc ttccaacgtt taatacagct
      961 ttctcataga gaaaacctga aaatccaagg aaaattccca tgacgagata tatccaatac
     1021 tggtctaggg tcatgggagg aatgttatct gacatatcca atactggtgt caaaccgaac
     1081 atgagtagag acacaaagtt tgctacgatg ctggctgcta gagttgagac ccagaaaaag
     1141 cgcgaaaaat ggtgatagac ttcttctaca acaaagagaa gtgctgcaat aggagcatta
     1201 aaggctgcgg ctaaacctgc tgcagctcca ctggcaatca aggaacgttc ctctactgga
     1261 ctggatttga gccacttggc aattccttta ccaccaactg ctccaagttg aatgctgggt
     1321 ccctctcgac ccagcatgag tccactggca atagcaagaa tacctagcac atattttttc
     1381 caaagaatgc cccaccagtt gagggacatg aggcctttca gttcggcttc gacttgagga
     1441 attcctgagc ctttaatatc tttttctgac cgtgttagtt tggcactgag ccaacagatg
     1501 agtatataaa acaaaaccag tacaaaaaga ttgcgcacta agtacccttg atcttgataa
     1561 actccttgta tcaggtggaa gcccttttca attaagaaac gaaaggatcc gacgataatt
     1621 ccaacgatga ggccgacaat gattcctcga ccaacttggg atagtatagt gctggatgca
     1681 aaggcgaatt ctttcttaga acggactatt tccgactgtt cctccatggt atttcctttc
     1741 aattaacttt cttttttacc taaaaccagt gatttaacgg gttcaaagct ggttcggtga
     1801 attggggtaa ctcctagttt tgtgaggcct tccagatgtt tagctgtgcc atatcctgca
     1861 ttagtagcga aatcatagcc agagaactgc tgatcgtatt ctttcatcaa ttcatcacgt
     1921 gttaccttgg ctactataga tgctgctgcg atagagaggg agttggcatc tcctttgata
     1981 atggaggttt gtgaaatggg caagtccagt ttcatggcat caatcaaaag gtgctctggt
     2041 tgagggctga gctgggagat tgcttcttgc atggctagtt tggttgcttc atagatgttg
     2101 acttggtcga tgacctgatt atctatgata ccaattccaa tcgacaaggc ttggtcttga
     2161 acggcttgga aaatctccag atgtttcttt ttaggaattt tcttgctgtc gttgagacct
     2221 ttaatcttac aatttttagg taaaataacg gccgcagcga ctacaggacc agcaagagga
     2281 ccacgaccaa cctcatcaat acctgcaatt aaggtcaatc cttgcttata aagttctttt
     2341 tcataagaaa gcatggattc caagcgcaaa ttttcatcta attcagcttg aatggctctt
     2401 ttacgcttgc tgatttcctt ttgaactcct gagcgattat ccttttcaag atctaaaaaa
     2461 atagggcttt ctaactcctt gactgtcaca aggagttctt tgatttcttt aatcgttgcc
     2521 atcgaggtct tccaatgtat ctaaggtata gttaccgagt ttgccatcac ggacttcctt
     2581 cacgaagaga ctgtaaaaac ggtcatagtc atcacggaaa ccgagggcgc gggtcatatc
     2641 cataataatc acaggcgctt cttcttcaat tttcatttgt ttgaagcgtt cagccagctt
     2701 ttctggataa tgttctttga aataattgat accaaaaatg gtaacctcat ccataggaag
     2761 caactgatcc ttgatagctc cagtcaatgc caacttaagt gcaacagttt catcctcaaa
     2821 cttaggccag agaatccccg gtgtatccaa gatttccaga tctttattgg ttttaagcca
     2881 ttgttgacct tttgtgaccc ctggcttgtt tccaacaaca gcaatctttt taccagccaa
     2941 acggttcatc agagtggatt taccagcgtt tggaatcccg ataatcatgg tacgcaaggt
     3001 ttcaatctga atcccacgtt ctttctggcg agcaatctta tcagccatga gcttcttggc
     3061 cgcatctgtt acaactttta cagtcacttg ctctttggag ttgatagcta gcgtctggat
     3121 tccttgtgat tcaaaatact gacgccattc cttggtcatt gctggatcag ccaagtcggc
     3181 cttgtttaaa atcaagagtt ttggtttatc accaacaatc ttggtcaaca taggattttg
     3241 actagataga ggcaagcgtg catctactaa aatcgtcaca aaatcaacaa attttaaatt
     3301 ctcctgcacc tgtcgacgag ctttagacat gtgaccagga aaccattgaa tagtagccat
     3361 tgagtttttt cctttcatag caaggattta gatcccctat tttattttac tatagtttga
     3421 acatagagag ttttcaaatg aatgatgtcc aaacagttct tgttttatga catagaaatc
     3481 tactttatat tatatcatgc cttctttatt ttgtccgatg atagcctttt accctatctt
     3541 ttaccctata ccctatccta taccctatat agaaaaaagc catcaaatga tgacttttca
     3601 gagggagcaa aaaactgtcc tctttatgtt ttaaacattt aacccaaatc attcatacct
     3661 ctctcaacta gatgtaactt acaaaacccc tgacctcatg agccactttc ttcctcctca
     3721 tgaggtcagt tttactttct gctgttccag tatcgttttt cctcgctaga tttcctcaaa
     3781 ag
//
LOCUS       DAVLYV010000120         3713 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLYV010000120 DAVLYV010000000
VERSION     DAVLYV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3713
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.120"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            62..280
                     /gene="nrdH"
                     /locus_tag="VYO70_002020"
     CDS             62..280
                     /gene="nrdH"
                     /locus_tag="VYO70_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1714174.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            361..2520
                     /gene="nrdE"
                     /locus_tag="VYO70_002021"
     CDS             361..2520
                     /gene="nrdE"
                     /locus_tag="VYO70_002021"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1714175.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            2708..3670
                     /gene="nrdF"
                     /locus_tag="VYO70_002022"
     CDS             2708..3670
                     /gene="nrdF"
                     /locus_tag="VYO70_002022"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1714176.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNCGLD"
BASE COUNT         1153 a          795 c          696 g         1069 t
ORIGIN      
        1 tgacaaaatt ttattttctg atatactaag atagtattaa attttgaaga ggagttacac
       61 aatggtaacc gtttattcta aaaacaattg tgtccaatgt aaaatgacca agcgtttctt
      121 ggacagtaat aatgtctctt atcgtgaaat caatcttgat gagcaacctg agtacgtcga
      181 tcaagttaaa gagctcggtt ttagcgcagc tcctgttatc caaacaccaa ctgaagtctt
      241 ttcaggtttc caaccaggaa aactgaaaca attagcataa tcttagtaca tcatccagaa
      301 gaaactgctt ttagggctaa cttagaagcc tttcttttgt aattagataa gggaaatttt
      361 atgggattaa aacatcttga agacgtgact tacttccgtc ttaataacga aattaaccgt
      421 cctgttaatg gacaaatcat gcttcataaa gataaggaag ccttggatgc tttctttaaa
      481 gaaaatgtag ttccaaacac tatggttttt gattcaatca aagacaaaat caattacctc
      541 attgaacaca actacatcga aacagccttt atcaagaaat accgtccaga gttcttggaa
      601 gaattggctc aatttatcaa agaccaaaac ttccaattca agtcattcat ggcagcttat
      661 aaattttaca atcaatatgc cttaaagact aatgacggtg aatactatct tgaaaatatg
      721 gaagaccgtg tcttcttcaa cgccctttat ttcgctgatg ggaatgaagc tgttgcaatc
      781 gatattgcca atgaaatcat ccaccaacgc taccaacctg ctactccttc cttcttgaat
      841 gctggacgtg ctcgtcgtgg ggagttggta tcttgtttcc taatccaagt gacggatgat
      901 atgaactcta tcgggcgttc tatcaactca gctcttcaac tttcacgtat tggtggtggt
      961 gtgggaatta ccctcagcaa ccttcgtgaa gctggtgcac ctatcaaagg ctatgaagga
     1021 gctgcttcag gtgtcgttcc tgttatgaag ctttttgaag acagcttctc ttactccaac
     1081 caattggggc aacgtcaagg tgccggcgtt gtctacctca acgtctttca cccagatatc
     1141 atcgctttcc tttcaactaa gaaagaaaac gctgatgaaa aagtacgtgt caagactcta
     1201 tcacttggtg ttgtagtacc tgataaattc tacgaattgg ctcgtaaaaa tgaagaaatg
     1261 tacctcttca gcccttactc tgtagaaaaa gaatacggtg tgccattcaa ttacatcgac
     1321 attactgaaa aatacgatga attggtcgca aatccaaata ttcgcaagac aaaaatcaag
     1381 gcgcgggatt tggaaactga aatctctaaa ttgcaacaag agtctggcta tccttatgta
     1441 gtcaacatcg atacggctaa ccgtgcaaat cctgttgatg gaaagattat catgagtaac
     1501 ttgtgttctg agattcttca agttcaagaa ccaagcttga tcaacgatgc tcaagaattc
     1561 cttcaaatgg gaacagatgt ttcatgtaac ctgggttcaa ccaacgtggt taacatgatg
     1621 acttcacctg attttggtcg ttctatccgt gctatggttc gtgcccttac tttcgttaca
     1681 gatagttcac atatcgtagc tgtaccaacg attgaccatg gaaatagcca agcccacacc
     1741 tttggtctcg gtgccatggg actgcacagc taccttgccc aacaactcat tgaatatggt
     1801 tcacctgagt ctgttgaatt tacaagcatc tactttatgc ttatgaacta ctggaccttg
     1861 gtagagtcaa acaatattgc acgtgaacgt ggtattacct tccacaactt tgaaaaatca
     1921 gactatgcta acggaagtta ctttgacaag tatgtaactg gtgaatttgt tccaacatca
     1981 gaccgtgtta aagaactctt caaaaatgtt tttatccctg gtgttgctga ttgggctgaa
     2041 cttcgcgaca aggttcaaga agatggtctt taccaccaaa atcgccttgc tgtagcgcca
     2101 aatggttcta tcagctatat caacgacgtt tctgcttcta tccacccgat tacgcaacgt
     2161 atcgaagaac gccaagagaa gaaaattggt aaaatctact accctgctgc tggcttgtct
     2221 acagaaacca ttccttacta cacttctgcc tacgatatgg atatgcgtaa agtcatcgat
     2281 gtttacgctg ctgcgactga acacgtggat caaggacttt cactcaccct cttcatgcgc
     2341 agtgacattc caaaaggcct ttacgaatgg aagagggaaa ataaacaaac gacacgtgat
     2401 ttgtccatcc ttcgtaacta tgcctttaac aagggaatca agtctatcta ctacgtccgt
     2461 acctttacag atgacggtgg agaagtcggc gccaaccaat gtgaaagctg tgtgatttag
     2521 tttctagcat agaaacatta tttttcccta agcagcactg ataaaatcaa atctactatg
     2581 tcataatata atttatgtaa cattttaaaa gtcgggcttc cgactttttg tgcgatactc
     2641 ctatagaatt atggtaaagt agaaataatt gtgagttcgc aatactaaac acagaaagag
     2701 attttaaatg gaaacttact acaaagccat taactggaat gccatcgaag atgtcatcga
     2761 caaatcaact tgggaaaaac tgacggagca attttggctc gatacacgta ttcccttatc
     2821 aaacgacttg gatgactgga gaaagctatc taacaaagag aaagacttgg tcggaaaagt
     2881 ttttggtggt ttaacccttc ttgacactat gcaatctgaa actggggttc aagcccttcg
     2941 cgcagacatc cgtacaccac atgaggaagc tgttttcaat aacatccaat ttatggaatc
     3001 tgtccacgct aaatcttact catcaatctt ttctaccttg aatactaagg ctgagattga
     3061 agaaattttc gaatggacca ataccaatcc ttacctacaa aagaaggctg agattgtcaa
     3121 cgaaatctac ctaaacggca gcccacttga aaagaaagtt gccagcgtct tcctcgaaac
     3181 cttcctcttc tactctggtt tcttcactcc cctctactat ctcggtaaca acaaactagc
     3241 caacgttgca gaaatcatta aattgattat tcgtgacgag tctgttcacg gaacctacat
     3301 tggttacaaa ttccaacttg gtttcaatga attgcctgaa gaagagcaag aaaaactcaa
     3361 agaatggatg tacgacctac tctatactct ttatgaaaat gaagaaggct acacagagag
     3421 tctttatgac ggtgttggtt ggactgaaga ggttaaaacc ttccttcgct acaatgccaa
     3481 taaagctctc atgaacatgg gacaagatcc acttttccca gattcagctg aagatgtcaa
     3541 cccaatcgtt atgaacggta tttcaacagg aacttccaac catgacttct tctctcaagt
     3601 cggaaatggt taccttcttg gcgaagttga agccatgcaa gatgatgact acaactgcgg
     3661 tttggactaa tactcttcga aaatcaaatt caaaccacgt cagcttcgcc ttg
//
LOCUS       DAVLYV010000121         3700 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLYV010000121 DAVLYV010000000
VERSION     DAVLYV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.121"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            34..>3700
                     /locus_tag="VYO70_002023"
     CDS             34..>3700
                     /locus_tag="VYO70_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1714177.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATSTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPAVQPELPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPKQVAPLPEYTGPQASAIVEPEQVAPLPEYTGVQAGSIVE
                     PEKVEAPKEYTGKIEQPSAEDTKPENEASSTNGESERPKDKIKEEKQVDKKLELRNVS
                     NVELYTVENNKYRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIESTTKNNKEVY
                     KIVAHAENLIQHENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNSDPNGTFHLGA
                     TMDAREVELPDDQESYVKNEFYGKLIGENNGKYYAIYNLKKPLFKTLNTATIQNLSIK
                     EANVSSKEDAATISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNSRISNSSFTGR
                     ITNTYDTTVGYEIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVGGIAGVVEKSA
                     TIKYSYVEGNVNNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDVNVTNGNAISG
                     DNFDNMKEDHAYSSKGNKVVNVVQVDDELVTKDSDVQRGTVLDADKVKEKKAELVSKH
                     STKVEDFDFTSRYTTIYNEVTGYQKSREQVYKNIEKLLPFYNRETIVKYGNLVDESSE
                     LFTKELLSVVPMKNNEVITDINKNKQEINKLLLHFEGNKSQVLNIAYKNDFSKVAEYS
                     IEYQGLLYTPNTLLHDYSNIVDNVLTDLNSVQYDSNAIKKILDISDKVKNTELYLDEQ
                     FVKTKANIKDTLSKLLSADAAIAENSNSIIDNYVIQKIKQNKEALLLGLTYLERWYNF
                     KYGETKAKDLVMYHLDFF"
BASE COUNT         1435 a          517 c          714 g         1034 t
ORIGIN      
        1 taacatatat aaattttatt tcaaggagga ataatggaaa agtattttgg tgaaaaacaa
       61 gagcgttttt catttagaaa attatcagta ggacttgtat ctgcaacgat ttcaagttta
      121 ttttttatgt ctgtattagc tagttcatct gtggatgctc aagaaactgc gggagttcac
      181 tataaatatg tggcagattc agagctatca tcagaagaaa agaagcagct tgtctatgat
      241 attccgacat acgtggagaa tgatgatgaa acttattatc ttgtttataa gttaaattct
      301 caaaatcaac tggcggaatt accaaatact ggaagcaaga atgagaggca agccctagtt
      361 gctggtgcta gcttagctgc tctgggaatt ttaatttttg ctgtttccaa gaaaaaggtt
      421 aagaataaaa cggtattaca tttagtattg gttgcaggaa taggaaatgg tgtcttagtt
      481 tcagtccatg ctttagaaaa tcatcttttg ctaaattaca atacggacta tgaattgacc
      541 tctggagaaa aattacctct tcctaaagag atttcaggtt acacttatat tggatatatc
      601 aaagagggaa aaacgacttc tgagtctgaa gtaagtaatc aaaagagttc agttgccact
      661 tctacaaaac aacaaaaggt ggattataat gttacaccaa attttgtaga ccatccatca
      721 acagtacaag ctattcagga acaaacacct gtttcttcaa ctaagccgac agaagttcaa
      781 gtagttgaaa aacctttctc tactgaatta atcaatccaa gaaaagaaga gaaacaatct
      841 tcagattctc aagaacaatt agccgaacat aagaatctag aaacgaagaa agaggagaag
      901 atttctccaa aagaaaagac tggggtaaat acattaaatc cacaggatga agttttatca
      961 ggtcaattga acaaacctga actcttatat cgtgaggaaa ctatagagac aaaaatagat
     1021 tttcaagaag aaattcaaga aaatcctgat ttagctgaag gaactgtaag agtaaaacaa
     1081 gaaggtaaat taggtaagaa agttgaaatc gtcagaatat tctctgtaaa caaggaagaa
     1141 gtttcgcgag aaattgtttc aacttcaacg actgcgccta gtccaagaat agtcgaaaaa
     1201 ggtactaaaa aaactcaagt tataaaggaa caacctgaga ctggtgtaga acataaggac
     1261 gtacagtctg gagctattgt tgaacccgca attcagcctg agttgcccga agctgtagta
     1321 agtgataaag gcgaaccagc agtccaacca gagctaccgg aagcggttgt aactgacaaa
     1381 ggtgagactg aggttcaacc agagtcgcca gatactgtgg taagtgataa aggtgaacct
     1441 aaacaggtag caccgcttcc agaatataca ggaccacaag ccagcgctat tgttgaacca
     1501 gagcaagtag caccactacc agaatacaca ggggtacaag ccggatcgat agtcgagcca
     1561 gagaaagtag aagcaccgaa agaatatact ggtaaaatcg aacaacctag tgcagaagat
     1621 actaaacctg aaaatgaggc aagctctaca aatggggaat cggaacgtcc taaagataaa
     1681 attaaagaag aaaaacaagt cgacaagaaa ctagaactta gaaatgtttc aaacgtggaa
     1741 ttatatacgg tagaaaataa taaatatcga catattacgg ctgtagatgg tgctttagat
     1801 agttcattga agtatttcat gaaggtaaaa tcagaaaatt ttaaagatat aatgttgcct
     1861 gttactaaaa ttgaaagtac aacaaaaaac aataaagaag tatacaaaat tgtggctcat
     1921 gcagaaaatc taattcaaca cgaaaacaat gttatttcga atgactatac atattattta
     1981 ccaaaaactc agcaaagtga aactggcgtc tatacatcat ttaaaaattt agttgatgca
     2041 atgaatagtg accctaatgg aacattccat ttaggtgcaa ctatggatgc tcgtgaagtt
     2101 gaacttccgg atgatcaaga aagttatgta aaaaatgaat tttacggaaa attaataggt
     2161 gaaaataatg gtaagtatta tgctatttat aacttgaaaa aacctttatt taaaactcta
     2221 aacaccgcta ctattcagaa tctttcaata aaagaagcga atgtttcttc taaagaagat
     2281 gctgctacaa tatcgaaaga agcaaaatac aatactctta tagataatgt tcattctgat
     2341 ggtattatag ctggtgaacg tggaattggt ggattggttt caaaagtaga taactcgaga
     2401 atttctaata gtagttttac aggcagaata actaacacat atgatacaac tgtagggtat
     2461 gaaattggtg gtttagtagg taaactttct ggttcgcttg cttctataga gaaatcaata
     2521 gcatcgattg acatagcctc taatgcaaaa tcaggagatc aaattgttgg tggtatagcc
     2581 ggagttgttg agaaaagtgc gacaattaag tatagctatg ttgaaggaaa tgttaacaat
     2641 gtgcgacact ttggtaaagt tgggggagtt gcaggtaatc tttgggatag agattcacaa
     2701 gatgtaagta aatcaggtaa actatcatat gttttaagtg atgttaatgt aacaaatggt
     2761 aatgctatat cgggtgataa ttttgataat atgaaagaag atcatgctta tagtagtaaa
     2821 ggcaataaag tagtaaatgt agtccaagta gatgatgaac ttgtgactaa agattcagat
     2881 gtacaaagag gtacggtatt agatgctgat aaagtaaaag agaaaaaggc agaattggtt
     2941 tccaaacaca gcactaaagt agaagatttt gattttactt caagatacac tactatttac
     3001 aatgaagtaa caggttatca aaagtcgaga gaacaagtat ataaaaatat tgaaaaacta
     3061 ttaccgttct ataatcgaga gacaattgtt aaatatggta atttagtaga tgaaagcagc
     3121 gagctattta ctaaagaact tctatcagtt gttcctatga agaataatga agtaataact
     3181 gatattaata aaaataaaca agaaataaac aaattgttat tacattttga aggtaataaa
     3241 tcacaagtat taaatattgc atataagaat gatttctcta aagttgctga gtatagtata
     3301 gagtatcaag gattattata tactcccaat actctattgc atgactacag taatattgta
     3361 gataatgttt taactgactt gaattctgta caatatgatt caaatgcaat taagaaaata
     3421 ttagatatct cagataaagt taaaaatacg gaattatact tagatgagca atttgttaaa
     3481 acaaaagcaa atataaaaga tactttatca aaacttctgt cagcggatgc agcaatcgct
     3541 gagaatagta attcgataat tgataattat gttattcaga aaatcaaaca gaataaagaa
     3601 gctttattgt taggattaac atacttagag cgttggtaca attttaaata tggtgaaacg
     3661 aaagcaaaag atttagttat gtatcacttg gatttcttcg
//
LOCUS       DAVLYV010000122         3543 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLYV010000122 DAVLYV010000000
VERSION     DAVLYV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.122"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(91..1227)
                     /gene="lctO"
                     /locus_tag="VYO70_002024"
     CDS             complement(91..1227)
                     /gene="lctO"
                     /locus_tag="VYO70_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1714178.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCNVENPNTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(1852..3342)
                     /gene="lysS"
                     /locus_tag="VYO70_002025"
     CDS             complement(1852..3342)
                     /gene="lysS"
                     /locus_tag="VYO70_002025"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1714179.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQSTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(3470..>3543)
                     /locus_tag="VYO70_002026"
     CDS             complement(3470..>3543)
                     /locus_tag="VYO70_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1714180.1"
                     /translation="QVIPKAAFGYIASGAEDTFTSFQ"
BASE COUNT         1017 a          760 c          695 g         1071 t
ORIGIN      
        1 ttatttcatt ttactatagt tttcgatagc aatttattct tccaatacac gaagaaaaac
       61 ctccacattc agtggaggca atctgtttta tcaatacaat tttaagtcac gagggtcaac
      121 tgggaaggtt gggttgtatg gattgtgacg gagcttgaag tgtttgacat cttcaatggt
      181 ctgagctcca gacaattgca taactgtctt caattccgca ttcaagtgtt caaagacttg
      241 acgcacaccg acactaccac cgagagccaa gccatagatg acagggcgtc caatagcaac
      301 caagtctgct cctgatgcca aggctttaaa gacgtgttga ccacgacgaa caccagagtc
      361 aaagacaatc ggcacacgtc tatcaactgc ttctgccact tcttgaagcg agtcaaaggc
      421 agctggtcca ccgtcgattt gacgaccacc gtggttggtt acccagatac cagaagctcc
      481 tgcagcaagc gaacgttcaa cgtcctcacg gcattgtggt cccttgacat acacaggaag
      541 accagagtat tcagcgataa attctacatc gcgtggagac aagcgttgtt tagctgattt
      601 gtaaacaaag tccattgatt taccagcacc ttctggcagg tattcttcaa caatcggcat
      661 gccaactggg aagacaaaac cattacgctt atccacttca cgattccccc ctacagtagc
      721 atctgccgtc aagacaatcg ctttataacc ttcagccttc acacggtcca tgatgtggcg
      781 gttgataccg tcatccttac taaagtaaaa ttgaaaccaa tgaggtgtcc cttgaagggc
      841 ttcagaaatc tctggaaggt caacagtaga gtaagaactg gttgtataaa gagaaccaaa
      901 ctcatgcaca ccacgcgcag tcgccacttc cccctgttca tttgccaatt tatgagccgc
      961 aacaggtgcc ataatgattg gagaagatag tttttcacct gcaaattcaa tctctgtatt
     1021 tggattttct acattgcaaa gtgtatgagg aacgatgagc ttgtggttaa aggcacggat
     1081 attctctctt aaagtgaaag tatcttccgc cccactagcg atatagccaa atgctgcttt
     1141 aggaataact tgttgcgcca ttggctccaa atcataggta ttgatgaagt ctacatgacc
     1201 ttctgcattg cttgttttgt atgacataaa ctatcctcct cgattaagta agcgtttgct
     1261 ttttactata caactataat aactcttttt tcaatcattt tcaagagttt tgcttaaaaa
     1321 ccatcttatt ttttcataca atatactagc cctactttcc ttaaactcta aaccttagtc
     1381 tatattaagt gaatcgttga gattttttgc tgccaggcat ttgaactact tttttatttg
     1441 ctgattctat ctactaatac tataaaaaaa caagtgaata agacaccaga acatcctcta
     1501 ataggaccta tggacttata tcagtcccat atatactgtt taagcaagat actatcctag
     1561 ttgacgctaa cttcctatcc cacttattaa ttttttattt atagtgaatc tgaacaaatc
     1621 aattggggaa tccaagtcaa tttctagtac tttttgaaaa gtttcagcgt actatcgtaa
     1681 aatgaaataa aacatgcaca aattgcttag ggaatttaaa ccaatttcta acaatgtctt
     1741 agaaatcaaa gtgtactatt tcaacttcaa tatactacac ttcattttct ccttaaattc
     1801 agtctctttt tgaatcaaaa aatcctctgg taagagccag aggataagaa tttatttcat
     1861 tgttgggaag agcaatacat cacggatagt tgttgtatca gtgaggagca tgcagagacg
     1921 gtcgataccg attcccaaac cacctgttgg tggcataccg tattcaagag cttcaatgta
     1981 gtcatagtcg attcctgtcg cttcatcatc accaagttct ttggctttag cttgggcttc
     2041 aaaacggcta agttggtcga ttgggtcgtt caactcagta aaggcattac cgtactcctt
     2101 agtcatgata aagagctcga aacggtcagt aaagcgttgg tcttcaggat ttttcttagc
     2161 gagtggagat acagctactg gatgtccata gacaaaggtt ggttggatta aagtttcttc
     2221 aacaaactct tcaaagaagg cattgatgat gtgaccaacc tcagtgtagt gtttctcaac
     2281 tggaactttc ttctcagcag cgatagcttt agcttcttcc aaagtcatgt cttgccagaa
     2341 atcgacacca gtaatttctc tgatagcatc caccatatga acacgcttaa atggttcgtt
     2401 aatcttgatt tcagtacttt ggtagttgac tgggccatca cctttgactg atttagcagc
     2461 gtgttggata atgccttcag tcaagtccat gatgtcttgg aagtctgcat aagcttggta
     2521 aacttcgata gaagtgaact cagggttatg agtagcgtcc attccttcgt tacggaagat
     2581 acggccaatt tcatagacac gttccatacc acccacaata aggcgtttca agtgaagctc
     2641 agtcgcgata cgaagcacca tgtcaatgtt ttgggcattg tggtgggtga taaatggacg
     2701 ggcagcagca ccaccggctt cattatgaag aacaggtgtt tccacttcaa ggaatccttt
     2761 ttggtcaagg taacgacgga tttcagagat gatttttgaa cgagtgacaa agcgttcaaa
     2821 gctttcacga ttagaaatca agtcaaggta acgtttacgg taaattgttt caacgtctgt
     2881 caaaccatgg aatttctcag gaagaggacg aagagcctta gacaagtgtg tgatgtgggt
     2941 tgccttgata gagagttctc ccatatccgt acgcatcact tcaccttcga caccaaggaa
     3001 gtcaccaagg tctgcttttt tgaagatttc gtagttttct tcaccgacag catccttacg
     3061 aacgtagatc tgaatctggc cttcgcggtc ttgaaggtgg gcaaaaccca ctttaccttt
     3121 accacgtttg gttaccaagc gtcctgcgat agtagctgtt tcgtttttat cgtgtaattg
     3181 ttctttatcg aggttggcat atttatcttt taattcttgt gaatttgcag tacgttcaaa
     3241 acgttttccg aaaggatcga ttccttgttc gcggagcgca gccatttttt cacggcgaac
     3301 gatctgctgg tcatttagtt cttccatatg ttctgtagac atgggatcct cctagtttta
     3361 ctcaaaattg aatacgatga gtcatttttt gcgatacccc cattttatca taaataagca
     3421 agaaattcac ggataatctt taaaaactaa aaagaacctg acgctaaaat cactggaaag
     3481 aagtgaaagt atcttccgcc ccactagcga tatagccaaa tgctgcttta ggaataactt
     3541 gtt
//
LOCUS       DAVLYV010000123         3372 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLYV010000123 DAVLYV010000000
VERSION     DAVLYV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.123"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            113..2746
                     /gene="polA"
                     /locus_tag="VYO70_002027"
     CDS             113..2746
                     /gene="polA"
                     /locus_tag="VYO70_002027"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1714181.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPVLLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYVQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            2831..3268
                     /locus_tag="VYO70_002028"
     CDS             2831..3268
                     /locus_tag="VYO70_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1714182.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT          906 a          595 c          873 g          998 t
ORIGIN      
        1 ctaggtttac taaattctct ggctctatct atttattcgg aaaaagtttg aaaaatacta
       61 gctctagctc ttcccaatgg tattttttga ttctttcctt tataatgggt gtatggataa
      121 gaaaaaatta ttattgattg atgggtcttc tgtagctttt cgggcgtttt ttgcgctgta
      181 tcagcagttg gaccgtttta agaatgcggc tggtttgcat accaatgcga tttatggttt
      241 tcagttgatg ttgagtcatt tgttggagcg ggttgagccg agtcatattt tggtggcttt
      301 tgatgcggga aagacgacct tccggacaga gatgtatgcg gactataagg gtggtcgggc
      361 caagactcct gatgagtttc gtgagcaatt tcctttcatt cgtgagttgc tggatcatat
      421 ggggattcgt cactatgagc tggctcagta tgaggcggat gacatcattg ggacgctgga
      481 taagctagca gagcaggatg gttttgatat taccattgtc agtggggaca aggatttgat
      541 tcagctgacg gatgagcata cggtggttga aatttccaag aaaggtgtgg ctgagtttga
      601 ggcctttacg ccagattacc tcatggaaga aatgggcctc acaccagctc agtttatcga
      661 tctcaaggcg ctcatgggtg ataagtcgga taatatccct ggggtgacca aagtcggtga
      721 aaagacgggt attaagctct tgctggagca tggttcgctt gaggggattt atgaaaatat
      781 tgatggaatg aagacttcta agatgaagga aaatctcatc aatgacaagg aacaggcctt
      841 tttgtctaaa acactagcga ccattgatac caaggcaccg attgcgattg gtttagagga
      901 cttggtctat agtggtccag atgttgaaaa tcttgggaaa ttttacgatg agatgggatt
      961 caaacagctc aagcaggctt taaatgtgtc gtcagctgat gtggctgagg gtttggattt
     1021 tactattgtt gaccaaatca gtcaagatat gctgagtgaa gagtctatct tccactttga
     1081 gctttttggt gagaattacc atacggataa tttggttgga tttgcctggt cttgtgggga
     1141 taagctctat gccacagaca agcttgagct gttgcaagac ccgattttca aggatttctt
     1201 agaaaaaaca tctctgagag tttatgactt taagaaggtt aaagttcttt tgcaacgttt
     1261 tggtgtggat ttgcaggcgc ctgcttttga catccgtttg gctaaatacc tcctttcgac
     1321 tgtggaggac aatgaaattg cgaccatcgc tagtctttat ggtcagactt acttggttga
     1381 tgatgaaact ttctacggta agggtgttaa aaaggccatt cctgaacgtg agaaattctt
     1441 ggaacactta gcttgtaaac ttgctgtttt ggtagaaaca gagcctgttc tacttgaaaa
     1501 actcagcgaa aatgggcaat tagagcttct ttatgatatg gagcaacctc tggcttttgt
     1561 tcttgccaag atggaaattg ctgggattgt ggtcaagaaa gagaccttgc ttgagatgca
     1621 ggctgaaaat gagcttgtca ttgaaaaact gactcaagag atttacgagc tggctggtga
     1681 ggagtttaat gtcaactcgc ctaagcagtt gggcgtgctt ctctttgaga aattgggact
     1741 tcctctagaa tacactaaga aaaccaagac aggttattcg acagcagtgg atgtgttgga
     1801 gcgtctcgct cctattgctc cgattgttaa gaaaatcctg gattaccgtc aaattgctaa
     1861 gattcaatct acttatgtaa ttggcttgca ggactggatt ttggctgatg gaaagattca
     1921 tactcgctat gtgcaggatt tgacccagac cgggcgtttg tctagtgtgg atccaaactt
     1981 gcaaaatatt cctgcccgat tggaacaggg gcgcttgatt cggaaggctt ttgtgccaga
     2041 gtgggaggat agtgtgctac tcagctctga ctattcacag attgaattgc gcgttttggc
     2101 gcatatttct aaggatgagc acttgattaa ggccttccaa gagggggcag atatccatac
     2161 ttcgacagcc atgcgggtct ttggcattga gcgtcctgat aatgtgactg caaatgaccg
     2221 tcgcaatgcc aaggcagtta actttggagt ggtttatggg atttcagact ttggcttgtc
     2281 taataatttg ggaattagtc gtaaggaagc caaagcctac attgatacct actttgaacg
     2341 ttttccaggt attaaaaact acatggatga agtggtgcgg gaggcgcgtg ataagggcta
     2401 tgtagagacc ctctttaagc gtcgccgtga gttgccagat atcaattcgc gcaacttcaa
     2461 tattcgtggt tttgcggagc gaactgctat caactcacct atccagggtt cggcagcaga
     2521 tattctcaag attgccatga ttcagctgga taaagccttg gttgcaggtg gttatcagac
     2581 taagatgctg ttacaagtgc acgatgaaat cgtccttgaa gtgcctaaat ctgaattggt
     2641 agagatgaaa aaattggtga aacaaaccat ggaagaagcc attcaactca gtgttcctct
     2701 tatcgcagat gagaatgaag gggcaacctg gtacgaggct aaataaaaag ggggctagtc
     2761 ctcctttttt gtagtagaat tctgcaaacc ttttcaaaat gtgctatact gatgaaaaag
     2821 gaggttttct atgagtcaag aatttatcaa tccaagtgat ggcgtgattc gtcagtatct
     2881 cgcaacgagt aaaacccttg ctgtggtggg tttgtctgac cgtgaagaaa caaccagcaa
     2941 tcgagtgaca aaggaaatgc aggctcgggg ctataaaatc atcccagtca atcccaaggc
     3001 ggcaggtggc gaaatcttgg gtgaaaaggc ttatgctagc ctcgctgaaa ttccttttcc
     3061 tgtagatatt gtcaatgttt atcgtcgcag tgaatttctg cccgatgtgg cgcgtgattt
     3121 tctcaaagct gatgccaaga ttttttgggc acagctagga cttgaaagtt tagaagcgaa
     3181 agagattttg cgtgatggtg gatgtgatga tatcgtgatg aatcgctgta tcaagagaga
     3241 acatactcgc ttgattgagg aagcataaga aaaaggtagc tgatgggcta ccttttgtgt
     3301 tatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcgggctgc tcaaaacact
     3361 gttttgaggt tg
//
LOCUS       DAVLYV010000124         3370 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLYV010000124 DAVLYV010000000
VERSION     DAVLYV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.124"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..900)
                     /gene="phtA"
                     /locus_tag="VYO70_002029"
     CDS             complement(<1..900)
                     /gene="phtA"
                     /locus_tag="VYO70_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1714183.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QYREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLS"
     gene            complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="VYO70_002030"
     CDS             complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="VYO70_002030"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1714184.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(2893..3156)
                     /locus_tag="VYO70_002031"
     CDS             complement(2893..3156)
                     /locus_tag="VYO70_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1714185.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT          888 a          728 c          634 g         1120 t
ORIGIN      
        1 actcaaaggc agtttgtaga gctgtttcaa gagactatca atgtcattac tttgacttgc
       61 ttgactgtta gtgttgctgt tgttgcttgt gttagtattt gtagttcctg gattgcttac
      121 agaaggtacc cagtttgttc ttgaagtgtt atcgctattt tgtcggcgat aggttcttga
      181 atttgacaga tttccccgac cagataggaa ggcttctgca gcagctaact cgctagctga
      241 taactcattc ttaggaatgt aatggtaatg gtcgccgtga ggaacgatat aagcatcacc
      301 agtatcctct atgatatcag aagcattaaa gatataacca tcatctgtag tatagcgtcc
      361 ttgcgaacgt gccaaggcaa cagctccatc gtttcttgga gtccctcctt cacgatactg
      421 actatgttct tgtttttgtc gattgatttc ctcttttgta cggacgttat ccgcgtgggc
      481 agcatcctta aggtaaacat agtattttcc atctaccttg ataacatatc cacccttgac
      541 ctcattaaca atatcctcat cttttagctt atagtttgga tctttcatga gtaattcttc
      601 actgaaaatc gcatcataag gaaccttacc attgtaataa tgatagtggt cgccatgtga
      661 agtgacatag ccttggtctg ttatcttgat gacgatttgc tcagcattga ttccttcacg
      721 cttgctaacc tcatcaggag tcaaattctc cgttttttgc gtcgcttgtt ttccatctat
      781 ataggaaaca cgattatttt ccttaaccgt tctagcttga tacagtccca actcgtaaga
      841 acaaacactt aaaatcaaag ctgccgcaga accaacaagg tatttcttat taattttcat
      901 cgaaactctc tttctatttg atatttctag ttgcaattca tttctaaatt gcagaaactc
      961 ctctctatta cttaactggt taatagttta ctcctaaatc tccaacttgt caagaatttt
     1021 atgaaccatt taagtatttt ttgttctgac cactactttt tcaaggttgg actttctata
     1081 aaaaagattt tcttgaaaat tctttttaaa atatcaaaaa aatccctgta actgagttgc
     1141 agggacttgt cgattaatca aaattaacgt attctttttg aagttcaaga acttcttcca
     1201 ttgttgagca ttctgtaagg gcacggtttg cgtactcttc catcttagct gtgtcgagtt
     1261 tcttcatcaa gctgcgtgta cgaagtacag atgttgctga catagagaac tcatccaagc
     1321 ccattccgac aagaagtgga acagcttgtt ggtcaccagc catctcacca cacataccag
     1381 cccatttacc ttcagcgtga gctgctttga tcacattgtt aatcaagcgt aggattgatg
     1441 ggttgtatgg ttggtaaagg tatgaaactt gttcgttcat acggtctgct gccattgtat
     1501 attggatcaa gtcgtttgta ccaattgaga agaagtcaac ttctttagca aattggtctg
     1561 caagcatagc cgctgcagga atctcaatca tgataccaac ttggatgtta tccgcaactg
     1621 caacaccttc agcaagaagg tttgcttttt cttcatcaaa gactgctttc gctgcacgga
     1681 attctttcaa gagcgcaacc attgggaaca tgatacgcaa ttgaccgtga acagacgcac
     1741 gaagaagagc acggatttgt gtgcggaaca tagcatctcc agtctcagag atagagatac
     1801 gaagagcacg gaatccaagg aatgggttca tttcgtgagg catatcgaag taaggaagtt
     1861 ccttatctcc accgatatcc attgtacgaa cgacaacagg tttaccgttc attccttcaa
     1921 gaacagcctt gtatgcttca tactgctcat cttcagttgg gaagtcttga gaatccatgt
     1981 acaagaactc tgtacggtaa agtccaacag cttctgcacc gttgttgtta acaccttcaa
     2041 cgtcttttgg agtaccaata ttggcagcca actcgaagtg tttaccgtca gcagtcactg
     2101 tttgagcatc tttcaaaagt gcccattcag ctttttgttt cgcataggct tcaccagctg
     2161 ctttaaattc tgccgcttgt tcatctgttg ggttgataat cacttctcca gtgatcccgt
     2221 taacagcaag gatgtcaccg tctttaacga tttcagtgat gttatttgta cctaatacag
     2281 cagcaatttc aagtgtacgt gccatgatag ctgagtggct tgtacgtcca ccaatgttgg
     2341 ttacaaaagc ttttacaaag tttttgtcca attgagctgt atctgaagga gtcaagtcat
     2401 gcgcaatcac aatcacttct tcattgatag aagctgggtt tggcaatttt ttaccaagaa
     2461 ggtttgccaa tacacgtttt gtcacgtcgc ggatatccgc tgcgcgttct tgcatgtatg
     2521 ggttgtcttc catgccttca aagatagtga taaacatatc tgtaacttct ttcagacctg
     2581 cttctgcatt cactttcttc gcacggatag tttccttgat ttggctgatc atttctgggt
     2641 cagcaagaac cattaagtga gcatcaaaaa cttgagctgc ttcttcaccg agcgtaccta
     2701 ctgctttctc gcgaataaca gaaagctcgt cttgtgatgc ctgtagagcg gcatcaaggc
     2761 gagcttcttc tgcgtttgta tcttcgactg taatagtctc aaatgacaaa tccggctgaa
     2821 cgagtagata tgcttttgca actgcaacac cgtcagatgc tgcgattcct ttaagcattt
     2881 ctgtcatttc ccttatgcca atccttcttt ttccattgtt tctgagattg cagcgatagc
     2941 gtcatctgca tctgcacctt cagctgagat agttacgtca gcaccttgac caacaccaag
     3001 actcataaca cccataattg atttaaggtt aactgattta cctttgtact caagagtgat
     3061 atctgaagca aatttgctag cagtttgtac caacaatgtt gctggacgtg cgtgaatacc
     3121 tgtttctgcc actacgtgga aatctttaga agccatagtt tgactctcct tttgttcttt
     3181 ctttttttga gttatatgtg ataaccctta caatttagta ttataccatc ttctaggatt
     3241 tttttcaagc attttctgga ttttccttca atttcctttc agataacttg ctgaaaatct
     3301 tctatcctag ataacaaaac acaggatata gtttcttcaa aaacaattta taggataatc
     3361 attgctattt
//
LOCUS       DAVLYV010000125         3337 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLYV010000125 DAVLYV010000000
VERSION     DAVLYV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.125"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            21..1412
                     /locus_tag="VYO70_002032"
     CDS             21..1412
                     /locus_tag="VYO70_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1714186.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIALPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            1555..2472
                     /gene="adcAII"
                     /locus_tag="VYO70_002033"
     CDS             1555..2472
                     /gene="adcAII"
                     /locus_tag="VYO70_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1714187.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            2480..>3337
                     /gene="phtD"
                     /locus_tag="VYO70_002034"
     CDS             2480..>3337
                     /gene="phtD"
                     /locus_tag="VYO70_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV1714188.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLS"
BASE COUNT          989 a          610 c          715 g         1023 t
ORIGIN      
        1 agtttatttt ggagtcaaag atgaatatat ttagaacaaa gaatgttagt ttagataaaa
       61 cagagatgca taggcatttg aagttatggg atttgatttt gctgggtatc ggagccatgg
      121 tagggacagg cgtctttaca atcacaggta ctgcagctgc aacacttgct ggcccagccc
      181 tagtgatttc aatcgttatt tctgccttgt gtgtgggatt atcagccctc ttttttgcag
      241 aatttgcctc gcgagtaccc gctacaggag gtgcctatag ttacctctat gctatcttag
      301 gagaattccc tgcctggttg gctggttggt taaccatgat ggagttcatg acagccatat
      361 caggcgtagc ttcgggttgg gcagcttatt ttaaaggctt gctctctcaa tacgggatag
      421 cccttcctca ggctttaaat ggtaccttta atcctcaagc agggacattt gttgatttat
      481 tgcctattct tgtcttggtc ttggtgactt cgcttgtttt actaaatgcc aaggcagcct
      541 tacgctttaa ttcgattcta gttattttga aattttccgc tttagctctc tttgttttag
      601 taggaatttg gaatattaag tttgataatt ggagcaattt tgctccttac ggttttggac
      661 aaatctatgg agctagcact ggtattatgg ctggtgcgtc cttgatgttc ttcggttttt
      721 tgggatttga atccatctct atggctgtag atgaagtcaa gactcctcaa aaaaatattc
      781 ctagagggat tgtcttatcg ctttctatcg taaccattct ctatgccttg gtgacccttg
      841 ttttgactgg tgtggttcac tatagtcatt taaatgtcga tgatgccgtt gcctttgccc
      901 ttcgtagtgt tgggattagt tgggcagcca actatgtgtc attagtggct atcttgacct
      961 tgattacagt ttgtatttcg atgacctatg ccctatcgcg tatgatttac agtttagcac
     1021 gtgacggctt agtgcctgct gcctttaagg aactgacgaa gactagcaag ataccaaaga
     1081 atgctactat cttgacaggt ctagcttcag cagtagcagc aggaatgttc ccactagcca
     1141 gtattgcagc ctttttaaat atttgtacct tagcctactt gatcatgttg gcttatggtc
     1201 tgattcgctt acggaaagaa aaaggaatgc ccaaagctgg ggaatttaaa acaccattgg
     1261 tacctctgtt accaatttta tcgatcatca tttgtttatc cttcatgtta cagtataata
     1321 tgaatacctg gattgctttc ctagttgcgt tgttagtagg aagtattatt tacttcactt
     1381 atggctataa gcattctacc atagaagaat aaagtgaaag tctattgagt tgttgacact
     1441 cagcagattt ttatatgtga aagaaatttc aaattttttc taaaaatatc ttgacagatt
     1501 aaattttcag gagtagaata tttactagtt aattaaatgg ttaaggagtt gttcatgaag
     1561 aaacaaaatt tatttttagt cctgttaagt gtctttcttt tgtgcttggg ggcttgtggt
     1621 caaaaggaaa gtcagacagg aaaggggatg aaaattgtga ccagttttta tcctatctac
     1681 gctatggtta aggaagtatc tggtgacttg aatgatgttc ggatgattca gtcaagtagt
     1741 ggtattcact cctttgaacc ttcggcaaat gatatcgcag ccatctatga tgcagatgtc
     1801 tttgtttacc attctcatac actcgaatct tgggcaggaa gtctggatcc aaatctaaaa
     1861 aaatccaaag tgaaggtctt agaggcttct gagggaatga ccttggaacg tgtccctgga
     1921 ctagaggatg tggaagcagg ggatggagtt gatgaaaaaa cgctctatga ccctcacaca
     1981 tggctagatc ctgaaaaagc tggagaagaa gcccaaatta tcgctgataa actttcagag
     2041 gtggatagtg agcataaaga gacttatcaa aaaaatgcgc aagcctttat caaaaaagct
     2101 caggaattga ctaagaaatt ccaaccaaaa tttgaaaaag cgactcagaa aacatttgta
     2161 acacaacata cagccttttc ttatctagcg aagagatttg ggcttaatca acttggtatt
     2221 gcaggtatct ctcctgaaca agaaccaagt ccacgacaac taacagaaat tcaggaattt
     2281 gttaagacct ataaggttaa aacgattttt acagaaagta acgcttcttc aaaagtagct
     2341 gaaactcttg tcaaatcaac aggtgtgggt cttaaaactc tgaatccttt agagtcagac
     2401 ccacaaaatg acaagaccta tttagaaaat cttgaagaaa atatgagtat tctagcagaa
     2461 gaattaaagt gaggaaagaa tgaaaatcaa taaaaaatat ctagcaggtt cagtggcagt
     2521 ccttgcccta agtgtttgtt cctatgaact tggtcgtcac caagctggtc aggttaagaa
     2581 agagtctaat cgagtttctt atatagatgg tgatcaggct ggtcaaaagg cagaaaattt
     2641 gacaccagat gaagtcagta agagagaggg gatcaacgcc gaacaaattg ttatcaagat
     2701 tacggatcaa ggttatgtga cctctcatgg agaccattat cattactata atggcaaggt
     2761 tccttatgat gccatcatca gtgaagaact tctcatgaaa gatccgaatt atcagttgaa
     2821 ggattcagac attgtcaatg aaatcaaggg tggctatgtg attaaggtag acggaaaata
     2881 ctatgtttac cttaaagatg cggcccatgc ggacaatatt cggacaaaag aagagattaa
     2941 acgtcagaag caggaacgca gtcataatca taactcaaga gcagataatg ctgttgctgc
     3001 agccagagcc caaggacgct atacaacgga tgatggttat atcttcaatg catctgatat
     3061 catcgaagat acgggcgatg cctatatcgt tcctcatggc aatcacttcc actatattcc
     3121 taagagtgat ttatctgcca gtgaattagc tgctgcccaa gcctattgga atgggaagca
     3181 gggatctcgt ccttcttcaa gttctagtca taatgcaaat ccagctcaac caagattgtc
     3241 agagaaccac aatctgactg tcactccaac ttatcatcaa aatcaagggg aaaacatttc
     3301 aagcctttta cgtgaattgt atgctaaacc cttatca
//
LOCUS       DAVLYV010000126         3297 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLYV010000126 DAVLYV010000000
VERSION     DAVLYV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.126"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..315
                     /locus_tag="VYO70_002035"
     CDS             1..315
                     /locus_tag="VYO70_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714189.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            999..1160
                     /locus_tag="VYO70_002036"
     CDS             999..1160
                     /locus_tag="VYO70_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714190.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            1252..2892
                     /locus_tag="VYO70_002037"
     CDS             1252..2892
                     /locus_tag="VYO70_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1714191.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWN
                     GGFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQN
                     GKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAIVSDEVWQAAQVKL
                     KSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKDGTKYKDFYYYGCK
                     HRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEKINMKVDTSEIEKE
                     IDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYRMYDKIDELESSLI
                     DAKAKKQTIEAEKLTGDNIYKILIYFDKLYKVMNDVERRQLISALISEIQVYEEKQSN
                     GQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            3145..>3297
                     /locus_tag="VYO70_002038"
     CDS             3145..>3297
                     /locus_tag="VYO70_002038"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714192.1"
                     /translation="MERLSMVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETE
                     DGFEVEY"
BASE COUNT         1297 a          408 c          690 g          902 t
ORIGIN      
        1 attggaatta catggaaaga tatagagata gataaagaga gagagggaga gatagataaa
       61 aagtccaccc ccatttttta tggagaattc aaaaatgtaa ggttaagcaa agaagaatat
      121 aaaaatctaa aagaaaaatt aaactcacac acagatataa tgataaataa actatccaga
      181 tacatggaaa gcagtggtaa gacctatcaa aaccactatg tgacaatctt aaaatggtat
      241 gaagaagata aagacaaact aagacagaaa ggtttaaata aaaaaatgaa ttatgatgta
      301 ggagaatctt tatagataaa aagaggtgga aaggcactat taaagacttt aagtctaaaa
      361 aggtatcaaa gtatgccaga gataaaaaaa tataaatatg acactttaaa ggcgattaga
      421 aaattaaaga aatgctagaa gacttttaga tgacagttac caagagggtc taattttcac
      481 tgctacttat gcagtgggaa tttgaccttt ttatataagc aaattctatt tcaaaattgg
      541 ttaccaagaa agacttcttt tcactgttaa gtataagagg gtaatttata gaattttatt
      601 acattaagat aaatattttc tgatggtcaa attttaaaaa gattaataac tctcgataaa
      661 ttttgaacct taacaattga atagaccaag acaagacgtt aattattttt ggagcgtaat
      721 aacctatccg tcaagatctt tcggctgaat aattcggcaa aaaaagttct gctaagtaaa
      781 aagaaagtga gcgataagta agagtttgaa tatagggagg gataccctat ccgctatgaa
      841 gaaaatgagg ataatgatac ttctaaggtt gaagccggct catccggaat agaggcggag
      901 gtgagattcc tatgttgcta atccaaagca cttgtcaatg tcaaaaaact taattaaaca
      961 aataattatt agatataaaa ctttagagga ggtatatatt ggacaatgac tggaatggat
     1021 tggctgattt aatagcaaat ctgattgcaa aatatgctgg tgctttagat ttagataatc
     1081 tacccgatcc aacgccagca aaaaatcaag agatgaaaaa tagttttgac atggcaaaaa
     1141 cacaaattga gacggactaa taaaagtgtt ataatatact tgaaatgata gtccaaactt
     1201 aaattaaaaa aattatatat gatatagaaa aattatattg aggttaatac tatgtcaaaa
     1261 gaaaaaataa aagtatacct ctatacacga gtatctacgt caatacagat agaggggtat
     1321 tcgttagagg cacaaaaatc acgaatgaaa gcttttgcta tttacaacga ttatgaaatt
     1381 gttggagagt atgaagatgc cgggaagtct ggcaagtcta ttgaaggtag aatacagttc
     1441 aatcgaatga tggaagatat aaaatctgga aaagatggag tatcttttgt tcttgtgttt
     1501 aagttatcaa gatttgcaag aaatgccgct gatgttcttt ctacacttca gataatgcag
     1561 gattatggag ttaatcttat ctgcgttgaa gatgatggca ttgattcatc taaagatgct
     1621 gggaaactaa tgatttctgt tttatcagct gtggctgaaa tagaaagaga aaacatccgt
     1681 attcaaacaa tggaaggtcg cattcaaaaa gcaagggaag gaaaatggaa tggtggcttt
     1741 gctccgtatg gatataaact tgaagacggc aagctgttta taaatgagga agaggcagtt
     1801 gcaataagaa cgattttcga ccagtatgta aatactacga taggagccaa tgggatatct
     1861 aaatacttag agaatcatgg aattagaaaa atcccaagac agaatggtaa gaatcctgta
     1921 tataatggga aaatagcatt tggaagaaga actttagaaa aagttcatgg tacaagaaat
     1981 gaatataagc aggttgaaca agatgaatat ctaatatctg aagggataca tgaagctata
     2041 gtttccgatg aagtttggca agctgctcag gttaagctaa aatctcaagc aaagaaatat
     2101 gagcatgtga ataaaggaaa agatacacgc acacacttgc tttctggaat tgtaaaatgc
     2161 ccgatatgtg gagtgggaat gtttgggaac aagtgtatca agaaaaagaa agatggcaca
     2221 aagtataaag atttttatta ctatggttgt aaacataggc agatgataag aggtcataag
     2281 tgtacattca gtaagcaaat tagagaagaa ttgttagatg atgctgttgc agaggtgatt
     2341 gtcaagatag taagtaatcc gaaatttgct tctatgatgc aagaaaaaat caacatgaag
     2401 gtggatacct ctgaaataga aaaagaaata gataattacc aaaaggaatt gagaaagagt
     2461 cattctacaa agtttaagct aattgaggaa atagataatt tagatgttga agataagcat
     2521 tataagagaa ggaaacagga tttagatgat agactatatc gtatgtatga caaaatagat
     2581 gaattagaat catcactaat tgatgcgaaa gcaaagaaac agactattga agctgaaaag
     2641 cttacaggag ataacatata taagattctg atctattttg ataagctcta taaagtcatg
     2701 aatgatgtag agcgtaggca gttaatttca gctttgattt ctgaaattca agtttatgaa
     2761 gaaaaacaat ctaacggaca atggctaaaa tccattactt ttaagctacc aattatagaa
     2821 gaaaatctca acataggttt ggacaatgat gaacaagttg aatgtgtaag tttattagag
     2881 aaacgtagtt aatcctcaaa aggttcccca aaccttttga gtcccactga cgcatcacgc
     2941 tgaggcggta tcactgcttg tacgagttga agcatcagcg aagtaaaagt agatgttcag
     3001 ccaatgcgac gaggtgagac cgaaggtcga taaggtgtgt tctgggtata ggactcgtaa
     3061 agtaagtagg aaatatgatg agatgataca gagagcctga attggtcagg tgacgtgctt
     3121 atttacatta gcctgataat gaaaatggag agattatcga tggttatatt ggaattgtac
     3181 caaaatgatt attctaaaga tttagtagca tttgattcca tagaagatgg gaaagccttt
     3241 gtagctcaaa tcccgggata cactctggaa acagaagatg gttttgaagt ggagtat
//
LOCUS       DAVLYV010000127         3247 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLYV010000127 DAVLYV010000000
VERSION     DAVLYV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.127"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(195..806)
                     /gene="rpsD"
                     /locus_tag="VYO70_002039"
     CDS             complement(195..806)
                     /gene="rpsD"
                     /locus_tag="VYO70_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1714193.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1001..2053)
                     /locus_tag="VYO70_002040"
     CDS             complement(1001..2053)
                     /locus_tag="VYO70_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1714194.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2050..2748)
                     /locus_tag="VYO70_002041"
     CDS             complement(2050..2748)
                     /locus_tag="VYO70_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1714195.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2915..>3247)
                     /locus_tag="VYO70_002042"
     CDS             complement(2915..>3247)
                     /locus_tag="VYO70_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1714196.1"
                     /translation="INVKETSDTANGSLSPSNSGSSVTPMNHNHAPGTTDSVPADTMT
                     SSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLG
                     LKNKKEEN"
BASE COUNT          909 a          730 c          622 g          986 t
ORIGIN      
        1 tctattgaat atgccataaa aggattatac cacattgtgt actatttttg gttcatttta
       61 ctatatcact ttggactata tcggtctagt tatgtctcaa cgattatagc tggatactaa
      121 gagaaatcaa aaaagcacta agggagaacg tcccaaagtg cttaatatca aagcctatct
      181 tattcaataa aatattacaa cattttgttg tagaattcaa cgacaagtgc ttcgttgatt
      241 tctgggttga tttcgtcgcg ttctggcaag cgagtcaatg aaccttccaa cttttcagcg
      301 tcgaatgata cgaatgctgg acgtccaaga gtagcttcta ctgcttcaag gattgctgga
      361 actttcaatg atttttcacg aactgagatt acttgacctg gagttacgcg gtatgatggg
      421 atatcaacgc gtttcccgtc aacaaggatg tgaccgtggt ttacgaattg acgagcttga
      481 cgacgagtag tcgcgagacc aagacggtaa acaacgttat ccaaacgacg ttccaaaaga
      541 agcataaagt tgaaacctag gattccgcct ttgatttttg tagcttgtac gaacaagtta
      601 cggaattgtt tttcacctac accgtaagtg aaacgaagtt tttgtttttc agccaattgc
      661 aaaccatatt ctgacaattt agaacggttg tttggtccgt gttgtcctgg tacgtagtta
      721 cgacgtgcca attctttacc tgtacctgta agtgaaaggc caaggcgacg agcttgtttc
      781 caagatggtc ctgtataacg tgacatatga atgtcctcct gatataaata atatttcggt
      841 ggaaatagtc acttagaaag ccctgattcg tgcagatgcc cttcgcctaa acagccaagg
      901 ttacttgtca taagacacct gttgacgagc ttcatgcttt cctgctgata tttcacacaa
      961 aatccatttt accatgaata gctggatttg taaaggggtt ttaggcttta ttttcactac
     1021 cagagagatt gagaacgagg gtaaaggtac ttcctagacc gtactggctg ctgactgtga
     1081 tttccccacc caattgatgg gccaattcac gcgcaatcgc aagtcctaat ccatgaccac
     1141 ctgtcttcat gttacgcgaa gtttcgacac gataaaggcg tttgaaaata ttttccaaat
     1201 cctctggggc aatgccctgc ccttcatcgg tcacactgat tgaaagctgg cccttctcca
     1261 gcttagtcac cacttccagc ttggttcctg gagcagaata tttaaaagcg ttatcgacca
     1321 gattcaccaa gatacgagaa agcttagcat aatctccctc aatccgggca gactctggga
     1381 ttacctgcaa gtggacatct cttctctcct gctcaatcaa aaactgaaat tcactcatgc
     1441 actcaattaa gagcttgtcc agaaaaatac tgtctttact ggtagtttcc acctgatttc
     1501 tagctgtgtt tagggtcaaa aaattcaact cctcaaccag tttattgagc ctctccgtct
     1561 ggcgtccaat ggttgctaga taatgagctt gctccgactc cttgataatc ccatccaaaa
     1621 tcccttctac cgtcgcttgg atcgaagtga taggggtctt aatatcatgc gacaactgag
     1681 caatcatcaa gcccttttct cgttcgcttt cttccaagga atcaaagctt acctgcaaat
     1741 catgggacat ctcattaaaa gtttgcccta attgctgaaa ttctacagga ccttgaacct
     1801 ccaaatttga aggaaaatcc ttggccgcta cccgcttggc atgctccttg agtttgccca
     1861 acgacgtaaa gactggcaat aggagaaaga gactaatccc agcaccgaca aggctggcaa
     1921 cgatggtcat cccaagcaaa aagtaaatct cgcctttcgc aatcagcatt ttttgaacag
     1981 cccaaaaaac aaccaaaatg gttaagaggg ttgaaataat atatccaacc aaaatataac
     2041 tttttagttt catgtttgtc ctctcggttt ctctatctta tatcccaacc cccaaactgt
     2101 cttaatagtg ggagtttggt cactactata ttttgccagc tcctgtcgaa gagcatggat
     2161 atgcacattc aaggtattgg tgtcatccac gtagtcttct ttccagatct tttcatagag
     2221 gtctgtcttg gagaaaactc gctctggatt gctagctaaa atccacagca attcaaatga
     2281 tttaacagtt aaatccagca tttcttctcc tatttgaact tcatgactac tatgattcat
     2341 ttttagattg ccaagggaaa tcagctctgt ttcgccccca cgatgaaggc ggcgcaaaat
     2401 attgtggaca cgcaaaacca gctcacgtgg gctaaaaggc ttagcaataa aatcatctgc
     2461 tcccaagctc aggccgtaaa tcttgtcctg ttcactggtc ttagcagtaa taaataggaa
     2521 aggctgctct ggtgataagt attgaacctc actgattaaa tcataaccat ccatccgagg
     2581 catcatgaca tctgtgataa tcaaatcaat cggttttttc ttgaacagct ctagcgcttc
     2641 cagtccatca tgggctacca agacctgata acctgcctga attaagtatc tctgatgaat
     2701 atctgtgatt tctacctcgt cgtcaacgag taaaattgtc tttcccatct gtctctcctt
     2761 tgataaaaac agtgctatac tgctttaagt ataacactat ttttattgtt ttacgatgat
     2821 tttaatctgc tattgaaaga aagtcctgat tgctgattga gttgttaaac taatcttagt
     2881 cagcccacta tccatcattt atttccttag ctgattagtt ttcttctttt ttgtttttca
     2941 aacctagacc accgagtaaa cctgcaagcg caagcccaag gaaaccaata cttgccattg
     3001 atgtttgagt ctcaccagta tttggtagca tagctttatc ctctgacatc atcgtatcag
     3061 acatcttgtt agcagaagca gccatgtttt cacctgccat cgtgttggta gaacttgtca
     3121 tggtgtcagc aggcacgcta tctgtagtac ctggagcatg attgtgattc atcggagtca
     3181 cgctagaacc agagttagaa ggtgataatg aaccatttgc tgtgtctgat gtttctttaa
     3241 catttat
//
LOCUS       DAVLYV010000128         3146 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLYV010000128 DAVLYV010000000
VERSION     DAVLYV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.128"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(188..916)
                     /locus_tag="VYO70_002043"
                     /pseudo
     CDS             complement(188..916)
                     /locus_tag="VYO70_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            1098..1247
                     /locus_tag="VYO70_002044"
     CDS             1098..1247
                     /locus_tag="VYO70_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714197.1"
                     /translation="MRFIVGRFTSFSLGIEFSPTSKLDDLLFKIAFLMILATWIKARK
                     TKEAT"
     gene            complement(1176..1487)
                     /locus_tag="VYO70_002045"
     CDS             complement(1176..1487)
                     /locus_tag="VYO70_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714198.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCLFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(1502..2788)
                     /locus_tag="VYO70_002046"
     CDS             complement(1502..2788)
                     /locus_tag="VYO70_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1714199.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESPEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAVQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          940 a          682 c          547 g          977 t
ORIGIN      
        1 aaaatatcta agacatcgct tctttgttta atttgatagg ctttaatacc taagctccga
       61 ctttatcact taaaaaataa aaaaggacag ctaatctctt gcgcatcaag ggatttcgcc
      121 attctttaat ctcgtttaag tgttgtatat ggagctttgc ataatttttt aaaactaaac
      181 aactacatta agcacgaact gtgacgctgg ttccatcttc cttataaaga ttgataagcc
      241 cctctttaag agctcgaacc atgtctccag cttgaacaag ttgtgggact ttaatagtca
      301 atagttccat tggatttgga gcgcggtcga ttttattgcc tttggcatca tgcaaatctt
      361 caatataggt ttcaaaatga cggaaacctg gaccataaaa ctcaacttgg tccccttcgt
      421 taatgacgtt ccgttgacga atggttgctg tttgtgtcgc atcatcataa gaaaccactt
      481 cagcgacaaa cttgtactca gggattttac gacgagcacc aaacaactgc tcattttcag
      541 atggtgtacc atagtaaaat cctgtagcca gttcacgttg ggcaaccttc cacatctcgt
      601 ccaccaagtc ttgtttgata gcttcaaact tttcaggact ttcaagatag gcatccacag
      661 ccgccttgta gcagttggtt actgttgata catagtgaat agactccata cgtccttcga
      721 tttttagact gtccacacca ttttcaatca tatctgagat atggtcaatc atagacatat
      781 caacggctga cattgaaaat tcttctggaa tctcaccctg caaactctta cgttctttcc
      841 caaatggcat atcgtaaagg tcgtacttcc aacggcatga ctgagaacat ccaccacggt
      901 tggcatcacg catactctag acggattgtt ttgacaattc taacctttta gactttgcat
      961 aataactgtt aaacagtcga aaattacccc agttttaaca agtagtcaaa ataccagata
     1021 aagtaaaaag gggctaggaa aaaagtcttt aaaatcttaa aaacgcatag tatcaggtat
     1081 tgaacaatct tgatattatg cgttttattg tgggaagatt tacttcattt tctcttggaa
     1141 ttgagttttc tcctacttca aaactagatg atttgctatt taaaatagcg ttccttatga
     1201 ttttggcaac ctggattaaa gcgagaaaaa caaaagaggc aacttaagct ttctttatat
     1261 tgcttatatg ttaatagttt tagacaaaca ccacataaaa taggcttatc ctgtatcaaa
     1321 gataagggat aaggctcaaa agggtgattt tctaatgaat catgacaccg ataacaagca
     1381 taatactttt tacaatcata gcattgtaaa gcaataatat ccagcttgct atggtaatga
     1441 atgcatcttg tttgcttatc cactaagaaa ccctgtgcct ctatcatttc cttttccttt
     1501 cttaagctcg aactgtgacg ctggttccat cttccttata aaaattgatg agtccttctt
     1561 ttaatgcacg aaccatgtct cctgattgaa cgggttgtgg gactttaata gtcaatagtt
     1621 ccattggatt tggagcgcgg tcgattttat ttcctttggc atcatgcaaa tcttcaatat
     1681 aggtttcaaa atgacggaaa cctggaccat aaaactcaac ttggtcccct tcgttaatga
     1741 cgtttcgttg acgaatggtt gctgtttgta ccgcatcatc ataagaaacc acttcagcga
     1801 caaacttgta ctcaggaatt ttacggcgag caccaaacaa ctgctcattt tcagatggta
     1861 taccgtagta gaaacctgtt gccaattcac gttgggcaac cttccacatc tcgtccacca
     1921 agtcttgttt gatagcttca aacttttcag gactttcaag ataggcatcc acagccgcct
     1981 tgtagcagtt ggttactgtt gatacgtagt gaatagactt catacgtcct tcgattttta
     2041 gactgtccac accattttca atcatatctg gaatgtggtc aatcatagac atgtcaacgg
     2101 ctgacattga aaattcttct ggaatctcac cctgcaaact cttacgttct ttcccaaatg
     2161 gcatatcgta aaggtcgtat ttccaacggc atgactgaga acatccacca cggttggcat
     2221 cacgcatact catgtggttt gaaagagtac aacgtccaga gtatgaaata cacatagctc
     2281 catggacaaa ggcttcaatt tcaacatctg tacgtttgcg gatctcagct aattcttcca
     2341 ttgaaacctc acgcgctaaa acgacacgag tcaagcctag ctctttccag aactcaaggg
     2401 tttcatagtt agtggcactg gcttgggtag aaaggtggat ttcaaggcct ggtgcttcag
     2461 tcgctgcaat cataatcaag gctgggtcag atacgataac tgctgcaatc ccgatatcac
     2521 gcagtttacg gaaccactca ccagcaccag cttcatttcc ttcgtgcata accatattag
     2581 ccgctacata gaccttggca ccatacttgg ccgcaaactg cacgccttct tccatctgtt
     2641 cgaaagtaaa gtttcccgca cggctacgaa gaccataggc ctgaccaccg ataaagacag
     2701 catctgctcc atactgaaca gctaccttta gcttctctaa agtccctgca ggtgataaaa
     2761 cctcaggacg ttttaatgtt tttgtcatga tttctcctat ttgcaatata aaagtttgac
     2821 atttttcctt ctcattttat agtaaataaa ggataaaatc aagcctagac ggattgtttt
     2881 gacaattcta atcttttaga ctttgcataa taactgttaa acagtcgaaa attaccccag
     2941 ttttaacaag tagtcaaaaa gtctaaaaaa gtaaaaacca gtatcccaag atactgactc
     3001 gtaaaaatac ataaattagt ccttagatga ctactcccac tctgttgctc ctataatgga
     3061 aaaggattga tacagtctag aatatcgctt ttttatttat tattctgata taaaactgtt
     3121 agaattgatt ttttgtttta taccca
//
LOCUS       DAVLYV010000129         3007 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLYV010000129 DAVLYV010000000
VERSION     DAVLYV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.129"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VYO70_002047"
     CDS             complement(27..1382)
                     /gene="rlmD"
                     /locus_tag="VYO70_002047"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1714200.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     V"
     gene            1420..2196
                     /gene="recX"
                     /locus_tag="VYO70_002048"
     CDS             1420..2196
                     /gene="recX"
                     /locus_tag="VYO70_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1714201.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2285..2818
                     /locus_tag="VYO70_002049"
     CDS             2285..2818
                     /locus_tag="VYO70_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1714202.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
BASE COUNT          966 a          542 c          540 g          959 t
ORIGIN      
        1 cttttcaaac tttgtcaact acttttttaa acttttttta ttaattttac aacagcttca
       61 gttcgagctg tatgtgggaa catatcgacc gactggatat aatgaagatc atagacttct
      121 actaagcgta ccaaatcacg agccaaggtc gaaacattac aagaaatata aaccattttt
      181 tctggtacat aagtaagaat agtatctaat aacttatcat ccagacctgt acgtggtggg
      241 tcaacaatca aagcatctgc tcggtagcct tccttgtacc aacgaggaat aatctcttct
      301 gccgttccag cttcataatg agtattgtca aatcccattc ttttagcatt tcgcttggca
      361 tcttcaatag cttctggaat aatatccata cctctgagtg tttttacttt ctttgcaaag
      421 gcaaatccaa tcgttccaac tccacaataa gcgtcaatca aatggtcttc tttatcaaca
      481 tccagcgctt ttactgcttc gctatagagg acttctgttt gctcaggatt tagttgataa
      541 aaagctcgag gggatagtga aaattcataa ttgagtacac cttcttgaat actctcttgc
      601 ccccagataa tctctgtctt ttcaccatat atctcactgg ttttagctgt atttgtatta
      661 acagctactg tcacaacttc tgggaaatct ttaaccaact cttttaccaa ttgagttaaa
      721 ttaagctggc ggtttgtaac aataataatc tgaacctgtc cggtctttct cgcgcgtcgg
      781 accataatag tacggacacc tagaactttt ctctcatccg tgattggaat ctggtgataa
      841 gtaagtaatt ctgctaagcg attagcaatc acttgggttt ccttatcttg taccaggcag
      901 tctttcaact ctactaaata gtgagagttt tgtgcatata agcccgcctt gacctgattt
      961 ttaaattttc gagtctgaaa ttgtaactta gctctgtaat attttggttc ctgcattcca
     1021 atagttggac gaatttcata attttcatat cctgcaggag caaatttttt cagcgcttga
     1081 tgaagtaagt ccgtcttgaa ctccagctgc ttatcataat gcaggtgcat gatttggcag
     1141 cctccgcatt cattataaat agtacaagat ggcacaattc gaaatttaga cttcttgttg
     1201 accttcagta attttgcttc aacaaagttg cgtctaatag aagtaatctg acaatagata
     1261 tcttcgcctt tgagagctcc tggtacaaag actaatgttt tttggtaaaa gccgattccc
     1321 tcaccgttaa ttcccatgcg cttgattttt aatggtattt tttgtttcac tttcagattc
     1381 atacccctat cttatcacat tttgagttat aatagagcta tgaaaatcac aaaacttgaa
     1441 aagaaaaaaa gactctatct gatggagctt gataatggcg acaaatgcta tatcaccgaa
     1501 gacacaattg ttcgttttat gttatcgaga gataaggtga taagcgaaga ggaattgaaa
     1561 gagattcaag actttgctca attttcttat ggtaagaatc tggccctcta ccacttatcc
     1621 tttaaagcac gtactgaaaa agaggtcaga gaatatctga aaaaatatga tattaatgaa
     1681 aacatcgttt ctcaagtcat tgctaatctt aaagaagaga agtggattaa tgatagccag
     1741 tacgcttatg ctatcatcaa tgccaatcaa ctttcaggag acaagggacc ttatgtactg
     1801 actcagaaat tagcacaaaa aggaatttca aaatctacta tagaagaaat cttgaatgat
     1861 tttgattttt cggaagttgc tcaacgtgta gccaataaac tattgaaaaa atatgaggga
     1921 aaacttccag ctcgtgccct gcaagataag attatccaga acttgactaa caagggcttc
     1981 tcttattttg atgctaaaat tgcctttgac gaattggata gtcaagttga tgaagaaacg
     2041 actcaggaac tcatcttcaa ggaacttgat aagcaatata ctaagtatgc ccgaaagtat
     2101 gaaggatacg aactgaagca gcgtttaact caagttttag cacgaaaggg ctacgatttt
     2161 tcggatatag caagcgctct tagagaatat ctttaatatt tccaagtgaa attcacagat
     2221 tttgggtaat tttatggtac aatagtaaat gataaactta taaattgtag aaagttggtt
     2281 agttatgaag cttccaaaag aaggcgactt tattacaatt caaagttata agcatgatgg
     2341 gagtctccac cgaacttggc gggacaccat ggtactaaaa acaacagaaa acgccattat
     2401 tggtgtcaac gatcatacac ttgttaccga aagtgatggt cgtcgttggg tcactcgaga
     2461 accggctatt gtttactttc acaagaaata ttggtttaat atcattgcca tgattcgtga
     2521 taatggaact tcttactatt gcaatatggc tagcccctac tatctggatg aagaagcact
     2581 gaagtatatt gattacgatt tggatgttaa aatttttaca gatggggaaa aacgtctctt
     2641 ggacgttgaa gagtacgagc gtcacaaacg caaaatgaat tattctgatg acttggacta
     2701 tattttaaaa gaacatgtca aaattcttgt tgattggatt aacaatggac gtggtccttt
     2761 ctcagaagcc tatgtaaaca tttggtacaa gcgctatgta gaactaaaga atcggtaaag
     2821 ttgtcaaagg tcaggaacaa aatcctgacc ttttttggta gtataaaata aattgtgtaa
     2881 acacaaaaag gaataaatct attatagcag agttacaaca cattactaga aagagattta
     2941 tgcaaaattc gtaaaatata taaaacaaat agatatactg ttatttttta atgtctttat
     3001 tccttgt
//
LOCUS       DAVLYV010000130         2997 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLYV010000130 DAVLYV010000000
VERSION     DAVLYV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.130"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..1208
                     /gene="dcm"
                     /locus_tag="VYO70_002050"
     CDS             <1..1208
                     /gene="dcm"
                     /locus_tag="VYO70_002050"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEV1714203.1"
                     /translation="LFAGIGGFRMGMEAQGHECLGFCEIDKFARKSYKSIFQTEGEIE
                     FHDIRDVSDDEFKKLRGKVDVICGGFPCQAFSIAGRRLGFEDTRGTLFFEIARAAKQI
                     QPRFLFLENVKGLLNHDKGRTFTTILTTLDELGFDVEWQVLNSKDFGVPKNRERVFII
                     GHSRKKGTRLLFPFRREGQATNSETLKALGNLNPSRSGMSGKVYYSEGLAPTLVRGKG
                     EGFKVAIPCMTPDRLDKRQNGRRFKENQEPMFTLNTQDRYGIVVVGDLPTSFKETGRV
                     YGSEGLSPTLTTMQGGDKIPKILIPEPIQFLKVREATKKGYAQAEIGDSINLERPSSQ
                     YRRGRVGKGIANTLTTSGQMGVVVASYEGEDKQVYQVAGVLIDGQFYRLRIRRITPKE
                     CFRLHGFPD"
     gene            1236..1346
                     /locus_tag="VYO70_002051"
     CDS             1236..1346
                     /locus_tag="VYO70_002051"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEV1714204.1"
                     /translation="MNSQLYKQVGNSVTVPVIAAIAKKLKEIEEKDKSIK"
     gene            1330..1779
                     /locus_tag="VYO70_002052"
                     /pseudo
     CDS             1330..1779
                     /locus_tag="VYO70_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645893.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1772..2152
                     /locus_tag="VYO70_002053"
     CDS             1772..2152
                     /locus_tag="VYO70_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1714205.1"
                     /translation="MIDLYLSKNSQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIGLPFVVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            2165..2398
                     /locus_tag="VYO70_002054"
     CDS             2165..2398
                     /locus_tag="VYO70_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1714206.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRHYKMEQRMPQSPWVERIVQILDLDYEDLFRR"
     gene            2401..2988
                     /locus_tag="VYO70_002055"
     CDS             2401..2988
                     /locus_tag="VYO70_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1714207.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQVLYGASLFS
                     FLWMYGQRKQVVKVNMKTRMKCLGIGLASLLIISLCFSLIHAQGSTNQTNLIGLQHQV
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALVHHPGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNFLVYSLLLF"
BASE COUNT          982 a          448 c          638 g          929 t
ORIGIN      
        1 atttatttgc tgggatagga ggctttagga tgggaatgga agctcaaggg catgagtgct
       61 tgggattttg tgagattgat aaatttgcta ggaaatctta taagtccatt tttcaaacgg
      121 aaggagaaat agaatttcat gacatacgag atgtttcaga tgacgaattt aaaaaactta
      181 gagggaaagt cgatgtcatc tgtgggggat tcccttgtca agcattttca atcgcaggaa
      241 gacgattggg atttgaagat actagaggaa ctttgttctt tgaaattgct cgggcggcca
      301 aacaaatcca accacgtttt ctttttcttg aaaatgttaa aggcctactc aatcacgata
      361 agggacggac gttcaccaca atacttacca cacttgatga gttggggttt gatgttgagt
      421 ggcaggtgct taacagtaag gattttggcg ttcccaaaaa cagagagagg gtgtttatta
      481 tcggacattc tagaaagaaa ggtaccagac tcttatttcc tttcagacga gaaggtcaag
      541 caactaactc tgagactttg aaagcactag gaaatttgaa tccatcaaga agtggaatga
      601 gcggtaaagt ctattattca gaaggtcttg cgccaacctt agttcgtggt aaaggagaag
      661 gatttaaagt tgcgattcct tgtatgacac cagatagatt agacaagaga caaaatggga
      721 gacgctttaa ggaaaatcaa gagccgatgt ttactttaaa tactcaggat cgttatggca
      781 ttgtcgttgt tggagattta ccgactagct ttaaggagac aggtcgtgtc tatggaagtg
      841 agggcttatc tccaacactg actacgatgc aaggaggaga taaaatcccc aaaatactaa
      901 ttccagaacc aatccaattt ctaaaagtac gggaagcaac gaaaaaagga tatgctcaag
      961 cagagattgg ggattcaatc aatttagaaa gaccaagttc tcagtatcgt cgtggtagag
     1021 ttggaaaagg tatagctaat acgttaacaa ctagtggtca aatgggagta gtagtggcta
     1081 gctatgaagg agaagataaa caagtttacc aagtagctgg tgttttaata gatggacaat
     1141 tttaccgttt gagaatacga cgaatcactc ctaaagagtg ttttcgattg catggctttc
     1201 ctgattaggc ttttgaagct gctagaaaag tctctattaa tagtcagctc tataaacaag
     1261 ttggtaatag tgtaaccgtt cctgtgattg ccgcaatcgc aaagaaatta aaagaaatag
     1321 aggaaaaaga taaaagcatt aaataaagaa tcaatactag attgtgatga attagaaaca
     1381 gaattacatg atgcagaaat caaacagctg gatgaacaaa tatttttgat gcccaattat
     1441 ccatgtgagt ttgaggtaac atttttagat gattaccata aaaaacataa ttacccacta
     1501 ttttatgaat cctatcttca aaacgttatg gaattccttg aaagtcagga tataaagaac
     1561 ggagttaatg cctttgtaga tgatcatcaa aatcttgttt ttgttttata ttgacaagac
     1621 tatcgagccg agggaaaaga ggggatactt acaacccaag taactgtaaa agcttatgat
     1681 gaagacaaga aatcgattaa cttctcaaag ttgttagatt ccttaatcgt ctcagaatat
     1741 caaatggaag caaatttgtt ggaggtctcc catgattgat ctctatctaa gtaaaaatag
     1801 ccaaagaaat caacttcttt tagacttctt ccaaaactat ggcattgagg tatcttgtca
     1861 ttcagtttct gaaatgacaa aggacaaatt aattgagatg atgagctatt cttcagattg
     1921 ttttgagttt ttatctccca atttattacg atttaagaac cgtgacaatc taagattaac
     1981 ggatttcgtt gagataattt taaaaaatcc tgaactaacc atcggacttc cttttgtggt
     2041 ttcaaataag cgggtttatc caagtctgaa tttggaagag gctagagctc tattgccaag
     2101 agatacgaaa caattgattt acatggcaca aacacattat ttatctaact aaaggagaag
     2161 taatatggct gaacgatttt gggagaactt gtccattatt ttggctgaaa gaaatatcag
     2221 ctggattgag ttaaccagaa aaatgtttgc gggagagttt cactatccaa gtgaattgaa
     2281 tcgtttatat caaaagattc gccactacaa gatggaacaa cgaatgcctc aaagtccatg
     2341 ggtagaaagg attgtgcaga tattagattt agattatgaa gatttatttc ggaggtaact
     2401 atgaaaagaa taattccagt ttatatattc caacaagtaa atgtcctatt ggtatctcta
     2461 tacttattga aatttctttg tatcggtgag ttaactatac tacaggttct ctatggtgcg
     2521 tctctttttt cttttttatg gatgtatggt caacgaaaac aagtggtcaa ggtcaatatg
     2581 aaaactagga tgaaatgcct tggtattgga ctagctagtc tactgattat aagtctatgt
     2641 tttagcctga ttcatgctca aggaagcacg aatcaaacaa atttaattgg ccttcaacat
     2701 caagttcctt ggttttcatt tttattgttc ttaatcaatg cgagtatggt tgaagaattt
     2761 ctgtatcgtg aaattttatg gaacttggtt agaaaattag atattcgagt tgctttgaca
     2821 agtgttttat ttgccttagt acatcatcca ggaaccattc tagcttggtg tctgtacgtt
     2881 tcacttggaa tgtttttagg tatggtgcgc tataaatctg acttatgggg aagtatgggg
     2941 ttacatttgg tgtggaactt tttagtttat agtttgctgc ttttttaaaa taggtga
//
LOCUS       DAVLYV010000131         2964 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLYV010000131 DAVLYV010000000
VERSION     DAVLYV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.131"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..1468
                     /gene="rlrA"
                     /locus_tag="VYO70_002056"
     CDS             <1..1468
                     /gene="rlrA"
                     /locus_tag="VYO70_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PI-1 pilus transcriptional regulator RlrA"
                     /protein_id="HEV1714208.1"
                     /translation="DIRSIELDELSTLTSLQSKSLLSILQELQETFEEELTFNLDTQQ
                     VQLIEHHSHQTNYYFHQLYNQSTILKILRFFLLQGNQSFNEFTQKEYISIATGYRVRQ
                     KCGLLLRSVGLDLVKNQVVGPEYRIRFLIALLQFHFGIEIYDLNDGSMDWVTHMIVQS
                     NSQLSHELLEITPDEYVHFSILVALTWKRREFPLEFPESKEFEKLKNLFMYPILMEHC
                     QTYLEPHANMTFTQEELDYIFLVYCSANSSFSKDKWNQEKKTHTIQLILQHTRGKHLL
                     SKFKNILGNDISNSLSFLTALTFLTRTFLFGLQNLVPYYNYYEHYGIESDKPLYHISK
                     AIVQEWMTEQKIEGVIDQHRLYLFSLYLTETIFSSLPAIPIFIILNNQADVNLIKSII
                     LRNFTDKVASVTGYNILISPPPSEEHLTEPLIIITTKEYLPYVKKQYPKGKHHFLTIA
                     LDLHVSQQRLIYQTIVDIRKEAFDKRVAMIAKKAHYLL"
     gene            1733..>2964
                     /locus_tag="VYO70_002057"
     CDS             1733..>2964
                     /locus_tag="VYO70_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1714209.1"
                     /translation="MEHINHTTLLIGIKDKNITLNKAIQHDTHIEVFATLDYHPPKCK
                     HCKGKQIKYDFQKPSKIPFIEIGAFPSLIRLKKRRFQCKSCRKVTVAETTLVQKNCQI
                     SEMVRQKIAQLLLNREALTHIASKLAISTSTSTVYRKLKQFHFQEDYTTLPEILSWDE
                     FSYQKGKLAFIAQNFNTKKIMTILDNRRQTTIRNHFFKYSKEARKKVKVVTVDMSGSY
                     IPLIKKLFPNAKIVLDRFHIVQHMSRALNQTRINIMKQFDDKSLEYRALKYYWKFILK
                     DSRKLSLKPFYARTFRETLTPRECLKKIFTLVPELKDYYDLYQLLLFHLQEKNTDQFW
                     GLIQDTLPHLNRTFKTTLSTFIRYKNYITNAIELPYSNAKLEATNKLIKDIKRNAFGY
                     RNFENFKKRIFIALNIKK"
BASE COUNT          976 a          625 c          463 g          900 t
ORIGIN      
        1 ggatattcgg tctattgaac tagatgaact gtctacttta acttcattac aatctaaaag
       61 tctcctaagc attttgcagg aattacaaga aacattcgag gaagagttga catttaattt
      121 agatacgcaa caagttcaat taatcgaaca ccattctcat caaacgaatt attacttcca
      181 tcagctttat aaccaatcaa ctattttaaa aatactccgc ttctttctac tacaaggtaa
      241 tcaaagcttt aacgaattta cacaaaaaga gtatatttcc atcgcaacag gctaccgcgt
      301 acgacaaaag tgtggcttgt tactacgttc agtaggtttg gaccttgtga aaaatcaagt
      361 agttggtcct gaatatcgca ttcgtttttt aattgcactc ctccagtttc attttggtat
      421 tgagatttat gatttgaatg atggaagtat ggattgggtc acacatatga ttgtgcagtc
      481 taattctcaa ctcagtcatg aactgttaga aatcacacct gacgagtatg ttcattttag
      541 catactagtg gcactcactt ggaaacgtcg ggaatttcca cttgagttcc ctgagtccaa
      601 agagtttgaa aaattaaaaa acctctttat gtatccgatt ttgatggaac attgccaaac
      661 ttacttagag ccacatgcca acatgacttt tactcaggaa gaactggatt atatcttttt
      721 ggtttattgt tctgctaatt cttcgttttc caaggacaaa tggaatcaag aaaaaaagac
      781 tcatactatt caactgattc ttcagcatac aagaggaaag catctactat ccaagttcaa
      841 gaatatctta ggaaatgaca tttcgaattc tctttctttt ttaacagcac tgactttctt
      901 aacaagaacg tttttgtttg gtttacaaaa tctagttccc tattataatt attacgaaca
      961 ttatgggatt gaaagcgaca agccactgta tcatatttcg aaagcaattg ttcaagagtg
     1021 gatgactgag cagaagattg aaggagttat cgaccagcat cggctgtacc ttttctcact
     1081 ctacttgaca gaaaccatct tttcctcact acctgctatc ccgattttta tcattttaaa
     1141 taatcaagca gatgtaaact taataaagtc cattatttta cggaatttta ctgacaaagt
     1201 tgcctctgtt acaggataca atattctaat tagtcctcct ccttcagagg agcatttgac
     1261 agagccattg attattatta caacgaaaga atacctgcct tacgttaaaa agcagtatcc
     1321 caaagggaaa catcactttt tgacgattgc cttagatttg catgttagcc aacagcgtct
     1381 catctatcag acaattgtag atatccgaaa agaggctttt gacaaacggg tcgcaatgat
     1441 tgctaaaaag gctcactatt tgttataagt tcatcgtact gtctacacac aaaaaggggc
     1501 ttcctaagcc atctctattc ttatccccat atttcccagt tttgggtaaa tctgaacaga
     1561 aaaacacttc tgctctctag tttctgctag tgaacaaaag tgttttttct ggctctataa
     1621 tttctgtagt gggtaaatcc cactgtggag attatagggc tttttgagta tcaaaaaagt
     1681 cccataagac ttataatgaa aagcgcctaa acaactcatt agaaagtatc ttatggaaca
     1741 catcaatcat accacattac tcatcggaat aaaagacaaa aacatcacct taaataaagc
     1801 cattcagcac gatacccata tcgaagtctt cgctacactt gattatcacc cacctaaatg
     1861 taaacactgt aaaggaaaac aaatcaaata cgacttccaa aagccttcta aaatcccttt
     1921 tatcgagatt ggtgctttcc ctagcctcat tcgtttgaaa aagagacgat ttcaatgcaa
     1981 gtcctgtcgg aaagtcactg tagctgaaac aactctcgtt cagaaaaatt gccaaatctc
     2041 ggaaatggtg agacagaaaa ttgcccaact cctactcaac agagaggctc ttacacatat
     2101 cgcttctaaa ttagccatct ctacctctac ctctaccgtc tatcgtaagc tcaagcaatt
     2161 tcatttccaa gaggattaca ccactttgcc tgaaatcctc tcctgggatg aattctccta
     2221 ccagaagggg aaattggctt tcattgctca gaatttcaac actaagaaaa tcatgaccat
     2281 tcttgataac agacgtcaaa caaccatccg aaatcatttc ttcaagtact cgaaagaagc
     2341 tagaaaaaaa gttaaagtcg tcactgttga tatgtctgga agttatatcc ctctcattaa
     2401 gaaattattt cccaatgcta aaattgttct cgatcgtttc cacattgtcc aacacatgag
     2461 cagggctctt aatcagacta gaatcaacat catgaagcaa tttgatgata aatctctgga
     2521 atacagagct cttaaatatt actggaaatt tatcctaaaa gatagtcgga aactctctct
     2581 taagcctttc tatgctagaa ctttcagaga gaccttaact cctagggagt gtctgaagaa
     2641 aatctttact ctagtacctg aacttaaaga ttactatgac ctgtatcaac tactcctatt
     2701 tcatctacaa gagaagaata ctgaccagtt ttggggctta attcaagaca cgttacctca
     2761 tctcaaccgc acctttaaaa ccactttgag cacatttatt cgctataaaa actacatcac
     2821 taacgccatt gaattgcctt attctaacgc taaacttgag gccactaaca aactcatcaa
     2881 agacatcaag cgcaatgcct ttggttatcg gaactttgaa aacttcaaaa aacggatttt
     2941 catcgctctg aacatcaaaa aaga
//
LOCUS       DAVLYV010000132         2949 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLYV010000132 DAVLYV010000000
VERSION     DAVLYV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.132"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(232..525)
                     /gene="rpmA"
                     /locus_tag="VYO70_002058"
     CDS             complement(232..525)
                     /gene="rpmA"
                     /locus_tag="VYO70_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1714210.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(542..886)
                     /locus_tag="VYO70_002059"
     CDS             complement(542..886)
                     /locus_tag="VYO70_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1714211.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(902..1216)
                     /gene="rplU"
                     /locus_tag="VYO70_002060"
     CDS             complement(902..1216)
                     /gene="rplU"
                     /locus_tag="VYO70_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1714212.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(1252..1339)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(1398..2033)
                     /locus_tag="VYO70_002061"
     CDS             complement(1398..2033)
                     /locus_tag="VYO70_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1714213.1"
                     /translation="MTFCAGYVDAYTFIVRGNILVAGQAGNVVFLSVELIQNNVSDIR
                     DKLLTLIAFMMGVFLLTIYKEKLRIVKKSILSLIPLAILSIIIGFVPQTVDNIYLVPP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLSMLFASWQKKVKEKASF"
     gene            2504..2803
                     /locus_tag="VYO70_002062"
     CDS             2504..2803
                     /locus_tag="VYO70_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1714214.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT          950 a          603 c          512 g          884 t
ORIGIN      
        1 aagtgcaaac taggaagtta gccgcaggct gtacttgagt acggcaaggt gaaactgacg
       61 tggtttgaat ttgattttcg aagagtatca atccactata catattaaaa gtatggatat
      121 caaaacaaaa aaacaggctc tccgaaaact cggaaagcct tattttaagc tgtttctagc
      181 ttcctcacct ttacatttca aggttcggga taaaaggttc aatggacctt tttatttagc
      241 gattgggtaa acagaaactt gttttttatc gcgtccttta cgttcaaagc gtactacgcc
      301 ttcaacttta gcgaacaaag tatcgtctcc accacgacca acgtttacac ctggatagat
      361 gtgtgtacca cgttgacggt aaaggattga tccacctgtt acagtttgtc cgtcagctgc
      421 tttagctcca agacgtttcg cttgtgaatc acgtccgttt gatgtagaac ctccaccttt
      481 tttgtgggcg aaaagttgca agttgttaag agtcattttt aacataatgt tttcctccgt
      541 gttagttttc tgtgataact ctggtttgga cgaactcaga agagttctcc gataagtttg
      601 ccatacctaa gaaaaatgat tcaaagaata actgggtcat ttctctctgg tgtgaaggaa
      661 gatcttttgg tatttcaacc atcagatagc caccttcatc ttcgtttaat tctaggattg
      721 gttcatagcc tgcaaatttc tcaatagaat tgataaagtt aatggcaagc gtagaaaccg
      781 atgcacacac gacatctaag ccgtattcgc cactctcggc gtgtccagta atttccgcac
      841 tcctcagctc gccatcttcg gctctctcaa agactgcctg tatcatgtgt tctccttaaa
      901 attaagcgtt gattgcgttg atgacaactt ttgtatatgg ttgacggtga ccttgtttac
      961 ggtggctacc ttttttaggt ttgtacttgt aagtaaccac tttcttttgt tttccttgtt
     1021 tttcaacagt tccaactaca gtagctccag caacaagtgg agttccgaca acagtgtttt
     1081 caccaccaac aagaacaact tcgttaaaag taacttcttg accagcttca acgttcaatt
     1141 tttcaacgta aactgcttga ccaacttcaa ctttaacttg ttttccgcca gttttgataa
     1201 ttgcgtatgt gctcattatg cacctcctat gatttttatg ggtttccccg attttttgtg
     1261 aagactcgcc tagcatcgtg ggacgaacca cttaaacttg aataatcaag caacgatgtt
     1321 cgtgcggttg cacaggatcg tgcatagtca actcttcaag tatagcatat ctcctatttt
     1381 cttacaagta ataacaccta aaatgaagct ttttctttta cttttttctg ccaagaggca
     1441 aaaagcatgc tgaggtaaaa aacgctcatc ataataggaa caccaagaat ggtcttttca
     1501 tgatagaaaa tcgtcaaata ggctgaaaag acaacgccaa ggacaaaact actaagcagg
     1561 ctaacaaata tgaatccttc acgcaaaaaa ggagtgtgct tggttcggaa ataatctcca
     1621 aaagccagca tggtccgttt gatattccct gtcataaaag cgttattata ggcaataccc
     1681 gacacttctc caaaagcagt tgtcaccagt cccatacaga aggccaaggg cggcactaga
     1741 tagatattat ctacagtttg tggcacaaaa ccaataatta ttgataggat tgctaaagga
     1801 atcaaggaca gaatagattt tttcacaatt ctcaattttt ccttataaat cgttaataaa
     1861 aagactccca tcataaacgc tattaaggtt aaaagcttat ccctaatatc cgaaacatta
     1921 ttttgtatga gctctaccga aagaaagaca acatttccag cttgtccagc tacaaggata
     1981 ttcccgcgaa caataaaagt gtaagcatcc acatatccag cacaaaacgt caaaaaagtg
     2041 ctaacctttt agactgacgt gatatttttc ttataggtaa taacctcatt ttccctccca
     2101 ttgtattttc tcttagaaat attgtaccat tttctttcta aaaaatcgta ggctaccatt
     2161 tagattttac tattagcata aaaataataa tagacaacta tttatccaaa aatagataga
     2221 ggtaacatgt ttgcaaacaa agcatacgaa cctttagtaa aatcatttcc atgaaactag
     2281 aatagagccc ctcttagcaa aaatcattat tttaatttat ttctaatcac tccttgacat
     2341 aaataactct caccaataaa agactatgtc ttaaaaaaat ggtataataa aatcaatact
     2401 tgggcttgat ggctatgcta ctaataacaa ttaggagaga aaatcaggca cttgttaaca
     2461 acaaggatta tccccttgag atgaaaggaa ctttagaaat cttatgatga acatgcaaaa
     2521 catgatgcgt caagcacaaa aacttcaaaa acaaatggaa caaagccaag ctgaacttgc
     2581 tgctatgcaa tttgttggca aatctgctca agatcttgtc caagcaacct taactggcga
     2641 taagaaagtt gtcagcattg atttcaatcc agctgtcgtt gacccagaag accttgagac
     2701 tctttctgat atgaccgttc aagccatcaa ctctgctctt gaacaaatcg atgaaactac
     2761 taagaaaaaa ctgggtgctt tcgctgggaa attgccattc taaaaacaag gagctagaac
     2821 aatgcttgtc gataacaaag gctaagaaag gtgcaaaaat tataaaaaac gcatagtatc
     2881 aagcaaccta aaactttggg ctctataata tttgtagtgg gtaaatcccc tatagatatt
     2941 atggagcct
//
LOCUS       DAVLYV010000133         2887 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLYV010000133 DAVLYV010000000
VERSION     DAVLYV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.133"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            229..1233
                     /locus_tag="VYO70_002063"
     CDS             229..1233
                     /locus_tag="VYO70_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEV1714215.1"
                     /translation="MNASFRGSASFIRDNVLVTAAHVVYDRDSRTEADSVYVIPAATP
                     NENPVGKIKVKEVRYLKEFQNTASNELTTYDLAVLILEEPIGAQLGTLGLPNNLGNLS
                     GTGATITGYPAVNSAKQMYTDSKDIVKDTGDFLMYQIDTLGGASGAGVYDSSFRLIGV
                     HVSGSSAGQMNSAVKLNEKALSFIYSVIQGHSIDGWKLISGTWYYYKNSTKQTGWQKI
                     NDTWYYLDASGKMRTDWEKVNGKWYYFESSGSMATGWKYVRGNWYYLDNKNGDMKTGW
                     YKDGSTWYYLDPTNGNMKKGWIKVGGNWYYLNSSGAMVTGSQTIDGKVYNFASSGEWI
                     "
     gene            1252..2232
                     /locus_tag="VYO70_002064"
     CDS             1252..2232
                     /locus_tag="VYO70_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1714216.1"
                     /translation="MKLLKKTMQVGLTAFFFGLLATNTVFADTTGGQFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGNKGEMVVGWQYLEIPGTGYRDNLLDNQPVNEIGLQHKW
                     YYFGQDGSLQEYVGWKELDAKDSLNVGKEYGEPFEGPEVLKYANYYFDKDHSLKTGWF
                     YDQSNWYYLAKTGYLGKDYLGGERRAGWIYDGSSWYYLDTTTAAMQTGWQYLGNNWYY
                     LRSSGAMATGWMKDASTWYYLDGQNGDMKTGWAYVGNSWYYLRSSGAMSTGWMKDGST
                     WYYLNASNGDMKTGWFQVNGKWYYAYGSGALAVNTTVGGYYVNYNGEWVK"
     gene            complement(2398..2604)
                     /locus_tag="VYO70_002065"
                     /pseudo
     CDS             complement(2398..2604)
                     /locus_tag="VYO70_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
BASE COUNT          961 a          370 c          603 g          953 t
ORIGIN      
        1 gctttgactg ttatttctag tacaggtgaa gaatatgagg taagtgaaac attacaagag
       61 agtccaggaa ctaataattt ttcacttccc gatatttcac cgacttatgg taaatattat
      121 acaaatcagt cagaagtcat tattggcaac aatgatttag ttaagattgg gaatacttta
      181 cagtatcctt attctacatc agcttacgta aaagcagaat ttaatagtat gaatgctagt
      241 tttagaggca gtgctagttt cataagagat aatgttttgg ttacagcagc tcatgttgtt
      301 tatgatcgtg attctagaac agaggcggat tctgtatatg ttatccctgc ggctactccg
      361 aacgaaaatc ctgtaggaaa aataaaggta aaagaagttc gttatctaaa agaatttcaa
      421 aacacagctt caaatgagct tactacgtat gatttagcag ttttgatttt ggaagaacca
      481 attggcgctc aattaggtac actaggtctt ccgaataatc tagggaatct ttcaggtaca
      541 ggggctacta ttactggata tcctgctgta aatagtgcta agcaaatgta tactgactct
      601 aaggatattg taaaagatac tggagatttt ctaatgtatc aaatagatac tctaggaggt
      661 gctagtggag ctggcgtata tgacagcagt tttagattga ttggtgttca tgtgtctggt
      721 agttctgctg gacagatgaa ttctgctgtt aagttaaatg aaaaggcttt atcatttatc
      781 tactctgtta ttcaaggaca ttctattgat ggttggaaat tgattagtgg tacatggtac
      841 tattataaga acagtactaa acaaacaggt tggcagaaaa taaatgatac ttggtactat
      901 ctagatgctt caggtaagat gcgtacagat tgggaaaaag taaatggaaa atggtattac
      961 tttgaatcat ctggttcgat ggcaacaggt tggaaatatg tacgaggcaa ctggtattac
     1021 ttagataata aaaatggtga tatgaagact ggttggtata aagatggttc aacatggtac
     1081 tatctagatc ctactaatgg aaacatgaaa aaaggttgga taaaagtagg aggaaactgg
     1141 tattatctaa attcaagtgg agcaatggtt acaggtagcc aaactatcga tggtaaagtt
     1201 tataatttcg cttcatctgg tgagtggatc taatattgga ggggtatata aatgaaactt
     1261 ttgaaaaaaa ctatgcaagt tggactaaca gcatttttct ttggtttgtt agctacaaat
     1321 acagtatttg cagataccac aggtggccaa tttgttgata aggacaatag aaaatattat
     1381 gtaaaagatg atcataaagc aatctattgg cataaaatag acggaaaaac ttactatttt
     1441 ggtaataaag gagaaatggt agttggatgg caatacttag aaattcctgg aacaggttat
     1501 cgtgataatt tattagataa ccaaccagtt aatgaaatag gccttcaaca caagtggtac
     1561 tattttggac aagatggaag tctccaagaa tatgttggtt ggaaggaatt agatgctaag
     1621 gattcattaa atgttggcaa agagtatggt gaaccatttg aaggtcctga agttcttaaa
     1681 tatgcaaatt attactttga taaagatcat tctttaaaga caggttggtt ttatgatcaa
     1741 tccaactggt actacttagc aaaaacaggt tatttaggaa aagactatct tggtggtgaa
     1801 agacgtgcag gttggatata tgacggttca agctggtatt atctagatac aacaactgct
     1861 gcaatgcaaa caggatggca atatctaggt aataactggt attatctccg ttcatcaggt
     1921 gctatggcaa ctggttggat gaaagatgct tcaacttggt attatctaga tggtcaaaat
     1981 ggtgatatga agacaggttg ggcttatgtt ggcaacagtt ggtactacct tcgttcatca
     2041 ggtgcaatgt ctactggttg gatgaaagat ggttcaactt ggtactacct aaatgcaagt
     2101 aatggagata tgaagacagg ctggttccaa gtcaatggta aatggtacta tgcctatggt
     2161 tcaggtgctt tagctgttaa taccacagta ggtggttatt acgtaaacta caacggtgaa
     2221 tgggttaagt aatgaaggct aatgttaaac tatagttgat acttaacata gtaaaagaga
     2281 gaatcagagt gtgattctct cttttgtaat ttaaaatgat tttaagaaag tttttatatg
     2341 tgtttgaaaa attgtataat ctatgtgatg gattaaattt ctacaagttt tatagagtca
     2401 aaaaataaca ctatcatcag ataatgttat ttttttgtta tcattataaa agtgtttagc
     2461 tatttctttt acatagtttt catattttgc tggaatttct gcatttgaaa tatagttttg
     2521 ataattaaaa ctttcatcaa ttgggtattc actatttact agactcataa aatcaaggat
     2581 tcttcttttt tcagcatcag aatcttaatt ttttgaagag agctgaagat aggggatcat
     2641 aatcttgtat actgattcaa aatatgttgt tggaacatca agtgttcttg caagagcaga
     2701 atgagttttt tgatctggaa cggatttact actttaccac ttgttgtatg aagttctatt
     2761 gatttttaat agattagata gctcagatta ggaatagtgt ttatctattt ggtgttcttt
     2821 aagacgaata ctagaaaata taagctaaag ctcctattgc tttattatgc aacaattatg
     2881 tcaaaat
//
LOCUS       DAVLYV010000134         2882 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLYV010000134 DAVLYV010000000
VERSION     DAVLYV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.134"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(152..430)
                     /locus_tag="VYO70_002066"
     CDS             complement(152..430)
                     /locus_tag="VYO70_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714217.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            complement(415..654)
                     /locus_tag="VYO70_002067"
     CDS             complement(415..654)
                     /locus_tag="VYO70_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714218.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            complement(1787..2515)
                     /locus_tag="VYO70_002068"
     CDS             complement(1787..2515)
                     /locus_tag="VYO70_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714219.1"
                     /translation="MSNVDKIRKIHIIVCWMYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNHVLTLI
                     FLIKICADTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            complement(2512..2865)
                     /locus_tag="VYO70_002069"
     CDS             complement(2512..2865)
                     /locus_tag="VYO70_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1714220.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGAYLSKRACQGICAALAMSPGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLAGAAGRIAYCIGYGALNRGCDIS
                     GNPYPWDGFISATVR"
BASE COUNT         1023 a          544 c          378 g          937 t
ORIGIN      
        1 aagataaaga cactactttt gcccttttcg attctctttt taaacatagc atcatcttcc
       61 ttcattccta taaaaacatt tccaatgaga gagcttattt tttaatttcg ttaaacaaca
      121 aaaaacgcaa gcttaagcct gcggacaatc tttatagcca tttcatttct ttcacatctg
      181 ttttacgaac tttagcatat ttcttagcgc ttgtaaaatg tagagtaact gatttaaata
      241 ctccgctttt gtctcttcgt aaacctaaaa ctgcggatct tttattttta tcctcaaaaa
      301 atatgacgtc caatttcata gtatttttag ataaatccct agctacgatg caacaactta
      361 ttattttaga atagaaaata tcaccgataa actcatataa agatatcctt tcgattacat
      421 cattgtacat aggatatttc taaaatcttc taaaataaaa acaccagctt gaatagcagg
      481 ttttgtttga ctagcataat ctcgtgtgat tttgtataag tcaaataaat gatgtaaatc
      541 agtcaagcaa aaattcactt tgaaaatctc taaatactta taattcgttg atacttgaca
      601 tgtttttcca ttgaatcttt cttgatagtc caaaatacaa tcaagtaatt tcaacgacaa
      661 actccatatt ttctcaaaat aaaaaagcac ggggcagttc tcactactgc ccaaagtggt
      721 tgattagagt cggcttctct aactccctat gttgtgctta ttaggcttat cgcaagggaa
      781 cagtttttag ctctgccaag ccacatagtg tgcttactac tatgtgtcca tgtcaccatg
      841 aattgccgaa cggtttaacg agaaagtata gtagattgaa acaagatgtg aacaaatcga
      901 ttaggaaagt caaattaatt tctagaaata ttttaacagt cataacggac tattccagat
      961 tcaatctact atattacttt taggtttatc ccaactcaat tatgacattt ttttcaaaag
     1021 tcaatatatc tcactctttt caacgacaag aaagaggctg ataatcgacc aacctctgtt
     1081 tttagataca gttcaagtcc aaactattct ttatagaaaa gtccgataaa ttcgagcaat
     1141 agttaacata cttacaaata taactcccaa aatagataac actattttca caaaactcag
     1201 ttccgaacct atgacatata tatgaaaaag aggggctagt cctacacata tatacaataa
     1261 gtagccaaca agttttatct caccaattcc tgcaaaaaaa tgtttcatgt gacgattctc
     1321 catttactcc attaaatcag ttttatttta gactatcata aaacaatcac ctcttcaata
     1381 aaaaccattt gcgaaatact attctgaagc attgatagct cagatttctc agaatagagc
     1441 tgtcctaact atcgagcagc tcaaatacat atatatgagc aattctaagc ctagaattgc
     1501 tataaaataa atgtttccag gctattctaa gtctttgact gcttaaaatt ttacattctg
     1561 aaggattcta aagctagaac agctcaaaat cacgagaact aaggtattcg aaacttcaga
     1621 gtaatacaat ccccatagga attctccatt cttattaagc atgattaaaa tgtaatccta
     1681 acaagtgatc ctcgactgtg tttgtagcac ttagttacct agataagact atttcccttt
     1741 atcatatctg attttaagcc gttctaagat ttaacctatg tttctattac ttgctatttt
     1801 taaataattc gtattccttt gctttgataa atggaactga aaaattagaa aaaagaagta
     1861 aaaccgaaaa caaatagcct gctttttgaa tcactaaaag atatcctaaa aataagtaag
     1921 ctataacatc caatgcgaaa agaaaaaata ttctcctctt cattaaagta cttgtgtcaa
     1981 ataatctgat gaataaaaat tttatcgtgg tatctgcaca aatttttatc aaaaagataa
     2041 gagtcagtac atgattatca actgtaccat aaagaataga tggaaatata gttaatataa
     2101 aactagaaat ctctaaatat tttatccaag gagattgaga caccttgctt ctaaactgac
     2161 taattttctt atattcgtta gtcaatttag tagcaaaatc ttccccaatt aaatgaaaga
     2221 ttgctaactc taggataaaa acacttatta atacggtata agtcaaaaaa ctttctggga
     2281 aaaacttatt gattaaaatg gggaaactca ttaacagaat cgaatttatg gctagacaga
     2341 tagatactat tttatttttc acagaatata gaggtagtga tactattgag tagataaaag
     2401 ctaaaaaaat aagcaaaaaa tactccgtgt catttataat cgccctgaat gataaaaata
     2461 tatacatcca acatacgata atgtgtattt ttcgaatctt atctacatta ctcatcttac
     2521 tgtcgcagat atgaatccat cccaaggata agggttcccg ctaatatcac aacctctatt
     2581 aagagcacca tatccaatac agtaagctat tcttcctgcc gccccagcca atacacctgc
     2641 tgctgcacca ataagccaac ctccaaggcc tccaacttta atatagttta ttagtttttt
     2701 ggttaaaact gcagctccag ctaatgctat aaaagttcct ggactcatag ctaaagctgc
     2761 gcaaattcct tgacaagcac gcttgcttaa ataagctccc ccatcaagat acatcatctc
     2821 ttcttgctca agagcaacat aattatttgg taatacgagt tccatttatt ttctcctttt
     2881 tt
//
LOCUS       DAVLYV010000135         2741 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLYV010000135 DAVLYV010000000
VERSION     DAVLYV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.135"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            16..558
                     /locus_tag="VYO70_002070"
     CDS             16..558
                     /locus_tag="VYO70_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1714221.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(599..672)
                     /locus_tag="VYO70_002071"
     tRNA            complement(599..672)
                     /locus_tag="VYO70_002071"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(636..638),aa:Asn,seq:gtt)
     gene            complement(678..749)
                     /locus_tag="VYO70_002072"
     tRNA            complement(678..749)
                     /locus_tag="VYO70_002072"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(714..716),aa:Glu,seq:ttc)
     gene            complement(801..1553)
                     /locus_tag="VYO70_002073"
     CDS             complement(801..1553)
                     /locus_tag="VYO70_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1714222.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            complement(1550..2674)
                     /locus_tag="VYO70_002074"
     CDS             complement(1550..2674)
                     /locus_tag="VYO70_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1714223.1"
                     /translation="MGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNFLSVTFSSIT
                     GDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLLLH
                     IVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQK
                     HLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEVLVKANHKLR
                     SDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMS
                     VLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRN
                     RGVGLNNVKELLDKYNNIILETEMEGSTFRKIIRFKREFE"
BASE COUNT          940 a          512 c          400 g          889 t
ORIGIN      
        1 ttacaaggag gaaatatgca agaaagtaac aaacgcttaa aaacaaagcg aactattgaa
       61 aatgctatgg tacaattact gatggaacag ccatttgata aaatttctac tgtcaaatta
      121 gtagaaaaag ccggaattag tcgttccagc ttctatactc actataagga taagtatgat
      181 atgattgagc actatcaaag caagctattc catacatttg aatatatttt tcaaaaacat
      241 gctcatcaca aaagagacgc tattttagaa gtttttgaat atctagagtc agaaccactt
      301 ctggctgccc ttctttctga aaatgggact aaagaaatcc aaaatttctt acgaaataaa
      361 cttcatatca tgcttagtac agatttacaa aagcgattta tgcaactgaa tctaaatacc
      421 accgaattag aatatagtag catctatcta actcatgcac tttttggtgt ctgccaaact
      481 tggattgcac atggaaaaaa agaaagttct caagaaataa cagacttcct tatgaaaatg
      541 cttggtgata caaattgata caaaaagagg aacacagctg tgttcccttt tatctatact
      601 ccgccagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta ctaccaactg
      661 agctatggcg gataaaatag tccgtacggg attcgaaccc gtgttaccgc cgtgaaaagg
      721 cggtgtctta accccttgac caacggacct tctatctgta gcagatataa ccattatatc
      781 aatttcttgc taattgtcaa tcacttttga gattttttct ctaaaatatc ttttaatttt
      841 ctaattttta atcttgaaat aggacaacga tggtcttcat agaaaacaat ttctaagttt
      901 tttcgatcaa tttctctgat attgcctata tttaccaaaa atgacttgtg aggagaataa
      961 aatcgctgag tatgtttgtc cttttcctga atatctgtca tggtaccata aaactctttt
     1021 gcaaaattct taccaataat gcgcaattta tgagagaccc ctgttgtttc aatatacaaa
     1081 atatcatggt aaggaatttt taaatcattt cctttgtaat tgtagtcgaa ataatctaca
     1141 acatcttcat tttcaagtaa catactcttc gtgtagaaga tattttgctc aattctcttc
     1201 ttaaacatct catcattgat atccttatca acaaaatcta gggctgatac ctggtattta
     1261 taggttagag tcgcaaactc tgatcgacta gtgataaaga cgataatagc gtaaggattg
     1321 taatgacgaa tgagctgagc cacttcaaat ccctttttct caattccatg aatatcgata
     1381 tctaggaaat aaagctgatt tacttcatca ttttcaatgt attcttcaaa ttcacggact
     1441 tttcccgttg tcttgtatga tattggaata ttcgattctt tcgaaatttc atccaatatt
     1501 ctctctagtc tcacttgatg ttcaataaca tcttctaaaa ttaaaacttt cattcaaatt
     1561 ccctcttaaa tctaatgatt tttctaaatg tactgccttc catctctgtt tctaaaataa
     1621 tattgttgta cttatctagt agttctttca cattatttaa tccgactccg cgatttcttc
     1681 ccttagtgga gaatcctaag gcaaatagat ctcctgaagg agtcatcgtc attttacatg
     1741 aattctgaat cacaataact gtttcagttt ccatcttaat aactgctact tccatctgct
     1801 ttttatagct atcagccgat ccttcgacag cattattcaa taaaacgctc atgatacgaa
     1861 ccaaatccaa tagttcaatt ggaagcttgg taatcgtatc ttttacttcc agtgtaaact
     1921 ctacaccatt atttcgagca tagacaattg actgagcaac caaacttcgt aaagctgagt
     1981 cttctatgtt gttcaaatca aagtaagtgt acttatctga acgcaattta tgatttgctt
     2041 tgactaaaac ttcattgtaa attctgtcaa tttcctgtaa attaccactg tcaattgcca
     2101 tctgcatgct gacaagcatt ccagcataat catgtcgaaa accacggatt tcattataca
     2161 gaccaacaat ttcatctgtg taattctgta aatgtttctg ttcaaatttc ttctgcttca
     2221 aagcaatctc tttctccatt tgaactttat gagaattcat tgcaaagaag gtcaaaagga
     2281 gagagataaa gacaatagat gacaaaatac ttccaaaact attcaaatgt ttaatcgtac
     2341 ttaccatatc tgaaacgaaa gatacaatat gtagcaatag taaagcaaaa aatacttttt
     2401 tcaagaaagg ataaaggtag tcctcgtcaa aataggctag ttccaaatgg aaatagtaaa
     2461 tgatttttaa tgtaacaaaa taggttaaca ccgtcacaac gaaaaagaat gggaaatgat
     2521 attgtaaaac aaaattatct cctgttatag aggagaaagt tacggacaga aagttatgag
     2581 tgctctcata taaaagagat agtagtaaac ttaggaatag tcctctatcc ctctcatact
     2641 gtttcatcca tcgaaaatag gaatataatc ccaaaggaaa taaaaatctt tcaatcccta
     2701 acccatctaa atatagaata tataagggaa aatcaaatac t
//
LOCUS       DAVLYV010000136         2705 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLYV010000136 DAVLYV010000000
VERSION     DAVLYV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.136"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..335)
                     /locus_tag="VYO70_002075"
     CDS             complement(<1..335)
                     /locus_tag="VYO70_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714224.1"
                     /translation="MTTKKANITIEEYIEMSEVDFNEAVNYEFTSDTCQLANSIYQSL
                     FKFFDKKNFSGDLIFTWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLN
                     MNRNGCIGD"
     gene            complement(349..1062)
                     /locus_tag="VYO70_002076"
     CDS             complement(349..1062)
                     /locus_tag="VYO70_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1714225.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1520..1894)
                     /locus_tag="VYO70_002077"
     CDS             complement(1520..1894)
                     /locus_tag="VYO70_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1714226.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGGIG
                     TSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            complement(2313..>2705)
                     /locus_tag="VYO70_002078"
     CDS             complement(2313..>2705)
                     /locus_tag="VYO70_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1714227.1"
                     /translation="DHVQRNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKP
                     ESPKPTEEPEEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLK
                     NNLLFGTQDNNTIMAEAEKLLALLKENK"
BASE COUNT          825 a          534 c          426 g          920 t
ORIGIN      
        1 aaatctccta tacagccatt acgattcata ttgaggctat attttcgatt agtaagataa
       61 ttcggaaata tatttcctat tactctaaga ttatctactt gtgaatccct tctcccaata
      121 taatcccctt ctttgactaa tgatggagat ttccaagtaa aaattaaatc gccagagaaa
      181 tttttcttat caaaaaactt aaaaagagat tgataaatac tatttgctaa ttgacaagtg
      241 tcagatgtaa attcataatt aacagcttca ttaaaatcaa cttcagacat ttcaatgtac
      301 tcttcaatag taatattagc tttttttgta gtcattagct aatctccttt aaaatgtgat
      361 cttttcaaga atatttttat ctaaacaatc cagcaagtct tggtaagaat agacttcgta
      421 agtcggctgg gcttgtgtgt gattttcgag gtgatgagga ttataccaga tagtgtcaat
      481 ccccgcatta ttgccacctt gaatgtcggc ggttagagaa tctccaatca tcagcgtctt
      541 ttctttacta aatccagcaa tttgctggcc aatcttttca taaaagagag catccggctt
      601 ttgagtttgc aactgttctg agataaagac ttgattgaaa taaggtacta gaccagattg
      661 agccaaacgt cctgtctgaa tggcagtaat gccatttgtc gcagcataca agtcataatc
      721 acgctcaatg aggctgtcca agagatcatg agcgcccgat agtgtttgtc cctgctgagc
      781 gagataaaat tggtaacgct gggcaagaaa actaccgtct ttttcctgtc caaaatgaga
      841 aaataaacga gaaaagcgcg tgttaaccag ctcttgttta ctgattttct tcagctccaa
      901 gtctttccag agagccttgt tcataggaac gtaataatct ttataagcct gaatatccgc
      961 aactccttct tcttttagaa gttgggtcaa agccacatcc tcagcagcat caaaatcaag
     1021 aagagtgtgg tcgaggtcga agagtagaaa tttgtagaac aatttgaggt tttcctttct
     1081 gaaaattcat taagaacatt atatcataaa gcacctcata caattaacta atttaatcac
     1141 ttaaaaaaat tcgaacactt tctatacaac tgacagctca aatctttcag aatagaacaa
     1201 tactaactat cgaacacccc gtcttcataa atacatatgt aattctaggc ctagaattcc
     1261 tataaactaa atgctttcat actcttccaa gtaattgatt gccttaaatt ttaatttttg
     1321 aaggattcta aagctagaat agccccatca caatcagttt tgattgattc acaatttaga
     1381 aacactatag tttcactcct gttaaaataa aaaggaactg cataaagcaa tccctttctg
     1441 attttgaaat catttactta acattttata gttgagataa tcaatagctt acctataaaa
     1501 agagttatag taaaattcct tatttattga tttcaagctc cgctaactgt gtttgaataa
     1561 ctgacagttc tgcaccagcc tgaagaagag cagctgcatt ataggcacct tctacaattg
     1621 gaaccctgtt gatgatgata cttttatcac tgaaatcagt caccattttt aagttcattt
     1681 tagcagaacc taggtcaaaa aaggcaagta aagtatctgc tggattttcg gaaacaaccc
     1741 tatctacttg atcaaaactc gttccaattc ctccgccctc ggttcctcct acataagtaa
     1801 tcggaacatc tttagctact ttactaatca gttcaacaac accttctgca atgtgtttgg
     1861 aatgtgaaac gataacaaga ccaatacttt ccatcaaacc accccagttt ctaaaatagc
     1921 agtaaatagt aatcctgatg agaatgatcc aggatcgata tgtccaaaaa accacatgct
     1981 cctaagacaa gagccaacag actggccatc aataatagta ttgttctttt ttatcattac
     2041 tccttaacta gtgtttaact gattaattag ccagtaaata gtttaccttt atttacacta
     2101 tctgttaaga tatagtaaaa tgaaataaga acaggacagt caaatcgatt tctaacaatg
     2161 ttttagaagt agaggtatac tattctaatt tcaatctact atattttgca cattttcata
     2221 aaaaaatgag aactagaact cacattctgc tctcattttt cgttttcccg ttctcctatc
     2281 ctgtttttag gagttagaaa atgctgctac ctttacttat tctcctttaa taaagccaat
     2341 agtttttcag cttctgccat aatagtattg ttgtcctggg tgccaaatag taaattattt
     2401 tttaatcctg tgagagtctc tttggcattg gacttgataa ttggatcctg gatttttcca
     2461 agtaaatctt cagcctctct cagtttttct tcaacctttt cagtctcgac ctgaggttct
     2521 tctgattcct ctggtgattc ttcttctggt tcctctgttg gttttggaga ctctggtttc
     2581 tcgctttgcg gtttctcttc tcgaggggtt tcttcctcag gtttttctgt ctgaggtttc
     2641 tcctcgcttg gtttttccgt ttgattggta tcagcttgac catttttgtt tctttgaaca
     2701 tggtc
//
LOCUS       DAVLYV010000137         2655 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLYV010000137 DAVLYV010000000
VERSION     DAVLYV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.137"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            239..2563
                     /gene="pflB"
                     /locus_tag="VYO70_002079"
     CDS             239..2563
                     /gene="pflB"
                     /locus_tag="VYO70_002079"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1714228.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT          845 a          549 c          518 g          743 t
ORIGIN      
        1 ttcgatttcc attgagtgtt actgcttatt ttcttttatt ataccctttt ttctgaaaaa
       61 aagaaaaaag gactttattt tttcaaaaat ataatacagt ttgaaataaa atatagactg
      121 ttttagaaaa gaaagtgtaa aaatagggaa ttttcacttg ttgaaatcgg ttactatatg
      181 gtatacttgt cttatgaatg taacagatga ctgttactag aaaaaagagg acattaatat
      241 ggttgttaag acagttgttg aagcacaaga tatttttgac aaagcttggg aaggcttcaa
      301 aggcgtagat tggaaagaaa aagcaagtgt atcacgattt gtacaagcta actacacacc
      361 ttatgatgga gacgaaagct tccttgcagg accaacagag cgttcacttc acatcaagaa
      421 aattgtagaa gaaactaaag cacactacga agaaactcgt ttcccaatgg acactcgtcc
      481 aacatctatc gctgatatcc ctgctggatt tatcgacaaa gaaaatgaag ttatcttcgg
      541 tatccaaaac gatgaactct tcaaattgaa cttcatgcca aaaggtggta tccgtatggc
      601 tgaaactact ttgaaagaaa atggatacga accagaccca gctgttcacg aaatcttcac
      661 taaatatgta acaacagtta acgacggtat tttccgtgcc tacacttcaa atattcgtcg
      721 cgctcgtcac gcacacactg taactggtct tccagatgca tactcacgcg gacgtatcat
      781 cggtgtttac gcacgtcttg ctctttacgg tgcagactac ttgatgcaag aaaaagtaaa
      841 tgactggaat gcaatcaaag aaatcgatga agaaacaatc cgtcttcgtg aagaagtaaa
      901 ccttcaatac caagcattgc aacaagttgt tcgcctgggt gacctttacg gggttgatgt
      961 tcgcaaacca gcgatgaacg tgaaagaagc aatccaatgg gttaacattg ctttcatggc
     1021 tgtctgccgt gtgattaacg gtgctgctac atctctaggt cgtgtaccaa tcgtattgga
     1081 catctttgca gaacgtgacc ttgctcgtgg tacatttact gaatcagaaa tccaagaatt
     1141 cgttgatgat ttcgttatga aacttcgtac agttaaattt gctcgtacaa aagcttatga
     1201 ccaattgtac tcaggtgacc caacctttat cacaacttct atggctggta tgggtaacga
     1261 cggtcgtcac cgtgttacta agatggacta ccgtttcttg aacactcttg acaacatcgg
     1321 taactcacca gaaccaaact tgacagttct ttggactgac aaattgccat acaacttccg
     1381 tcgctactgt atgcacatga gccacaaaca ctcttctatc caatacgaag gtgtaacaac
     1441 aatggctaaa gacggatatg gtgaaatgag ctgtatctca tgctgtgtgt ctccacttga
     1501 tccagaaaat gaagaacaac gccacaacat ccagtacttc ggtgctcgtg taaacgttct
     1561 caaagccctt cttactggtt tgaatggtgg ttacgacgat gttcacaaag actacaaagt
     1621 atttgatatc gaaccaatcc gtgacgaagt tcttgaattt gaatcagtta aagcgaactt
     1681 tgaaaaatct cttgactggt tgactgacac ttacgtagat gccttgaaca tcatccacta
     1741 catgactgat aggtacaact acgaagctgt tcaaatggcc ttcttgccaa ctaaacaacg
     1801 tgccaacatg ggattcggta tctgtggatt tgctaacact gttgatacat tgtcagctat
     1861 caaatacgct acagttaaac caatccgtga cgaagatggc tacatctacg attacgaaac
     1921 aatcggtgac tacccacgct ggggtgaaga tgacccacgt tcaaacgaat tggcagaatg
     1981 gttgatcgaa gcttacacaa ctcgtctacg tagccacaaa ctatacaaag acgcagaagc
     2041 tacagtatca cttttgacaa tcacatctaa cgttgcttac tctaaacaaa ctggtaactc
     2101 accagttcac aaaggtgtat acctcaacga agatggttct gtgaacttgt ctaaacttga
     2161 attcttctca ccaggtgcta acccatctaa caaagctaaa ggtggttggt tgcaaaactt
     2221 gaactcactt tctagccttg actttagtta tgcagctgac ggtatctcat tgactacaca
     2281 agtatcacct cgcgctcttg gtaagactcg tgatgaacaa gttgataact tggtaacaat
     2341 ccttgatggt tacttcgaaa acggtggaca acacgttaac ttgaacgtta tggacttgaa
     2401 cgatgtttac gaaaaaatca tgtcaggtga agacgttatc gtacgtatct ctggatactg
     2461 tgtaaatact aaatacctca ctccagaaca aaaaactgaa ttgacacaac gtgtcttcca
     2521 cgaagttctt tcaatggatg acgccttgga tgcattgagc taatcaagtt cttgaataat
     2581 aaaaagggct ctttgtcaac tgtagtgggt tgaagaaaag ctaagctcga gaaaggacaa
     2641 attttgtcct ttctt
//
LOCUS       DAVLYV010000138         2638 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLYV010000138 DAVLYV010000000
VERSION     DAVLYV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.138"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..2585
                     /locus_tag="VYO70_002080"
     CDS             <1..2585
                     /locus_tag="VYO70_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1714229.1"
                     /translation="FSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDGSQ
                     SSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSEEL
                     LMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEHVK
                     DNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLSAS
                     ELAAAKAHLAGKNMQPSQLSYSSTPSPSLPINPGTSHEKHEEDGYGFDANRIIAEDES
                     GFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSNAKEMK
                     DLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVGIGHSH
                     SNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVSFSFPP
                     ELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFKYTIAS
                     KDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDALVRVFD
                     EFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYIVEVPI
                     LEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTSNSTLE
                     EVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTGEAPQG
                     NGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPEGNVGS
                     DPMLDSALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVIKKNLS
                     DLIA"
BASE COUNT         1003 a          413 c          526 g          696 t
ORIGIN      
        1 aatttagtaa aaaatatata gcagctggat cagctgttat cgtatccttg agtctatgtg
       61 cctatgcact aaaccagcat cgttcgcagg aaaataagga caataatcgt gtctcttatg
      121 tggatggcag ccagtcaagt cagaaaagtg aaaacttgac accagaccag gttagccaga
      181 aagaaggaat tcaggctgag caaattgtaa tcaaaattac agatcagggc tatgtaacgt
      241 cacacggtga tcactatcat tactataatg ggaaagttcc ttatgatgcc ctctttagtg
      301 aagaactctt gatgaaggat ccaaactatc aacttaaaga cgctgatatt gtcaatgaag
      361 tcaagggtgg ttatatcatc aaggtcgatg gaaaatatta tgtctacctg aaagatgcag
      421 ctcatgctga taatgttcga actaaagatg aaatcaatcg tcaaaaacaa gaacatgtca
      481 aagataatga gaaggttaac tctaatgttg ctgtagcaag gtctcaggga cgatatacga
      541 caaatgatgg ttatgtcttt aatccagctg atattatcga agatacgggt aatgcttata
      601 tcgttcctca tggaggtcac tatcactaca ttcccaaaag cgatttatct gctagtgaat
      661 tagcagcagc taaagcacat ctggctggaa aaaatatgca accgagtcag ttaagctatt
      721 cttcaacacc ttctccatct cttccaatca atccaggaac ttcacatgag aaacatgaag
      781 aagatggata cggatttgat gctaatcgta ttatcgctga agatgaatca ggttttgtca
      841 tgagtcacgg agaccacaat cattatttct tcaagaagga cttgacagaa gagcaaatta
      901 aggctgcgca aaaacattta gaggaagtta aaactagtca taatggatta gattctttgt
      961 catctcatga acaggattat ccaagtaatg ccaaagaaat gaaagattta gataaaaaaa
     1021 tcgaagaaaa aattgctggc attatgaaac aatatggtgt caaacgtgaa agtattgtcg
     1081 tgaataaaga aaaaaatgcg attatttatc cgcatggaga tcaccatcat gcagatccga
     1141 ttgatgaaca taaaccggtt ggaattggtc attctcacag taactatgaa ctgtttaaac
     1201 ccgaagaagg agttgctaaa aaagaaggga ataaagttta tactggagaa gaattaacga
     1261 atgttgttaa tttgttaaaa aatagtacgt ttaataatca aaactttact ctagccaatg
     1321 gtcaaaaacg cgtttctttt agttttccgc ctgaattgga gaaaaaatta ggtatcaata
     1381 tgctagtaaa attaataaca ccagatggaa aagtattgga gaaagtatct ggtaaagtat
     1441 ttggagaagg agtagggaat attgcaaact ttgaattaga tcaaccttat ttaccaggac
     1501 aaacatttaa gtatactatc gcttcaaaag attatccaga agtaagttat gatggtacat
     1561 ttacagttcc aacctcttta gcttacaaaa tggccagtca aacgattttc tatcctttcc
     1621 atgcagggga tacttattta agagtgaacc ctcaatttgc agtgcctaaa ggaactgatg
     1681 ctttagtcag agtgtttgat gaatttcatg gaaatgctta tttagaaaat aactataaag
     1741 ttggtgaaat caaattaccg attccgaaat taaaccaagg aacaaccaga acggccggaa
     1801 ataaaattcc tgtaaccttc atggcaaatg cttatttgga caatcaatcg acttatattg
     1861 tggaagtacc tatcttggaa aaagaaaatc aaactgataa accaagtatt ctaccacaat
     1921 ttaaaaggaa taaagcacaa gaaaactcaa aacttgatga aaaggtagaa gaaccaaaga
     1981 ctagtgagaa ggtagaaaaa gaaaaacttt ctgaaactgg gaatagtact agtaattcaa
     2041 cgttagaaga agttcctaca gtggatcctg tacaagaaaa agtagcaaaa tttgctgaaa
     2101 gttatgggat gaagctagaa aatgtcttgt ttaatatgga cggaacaatt gaattatatt
     2161 taccatcggg agaagtcatt aaaaagaata tggcagattt tacaggagaa gcacctcaag
     2221 gaaatggtga aaataaacca tctgaaaatg gaaaagtatc tactggaaca gttgagaacc
     2281 aaccaacaga aaataaacca gcagattctt taccagaggc accaaacgaa aaacctgtaa
     2341 aaccagaaaa ctcaacggat aatggaatgt tgaatccaga agggaatgtg gggagtgacc
     2401 ctatgttaga ttcagcatta gaggaagctc cagcagtaga tcctgtacaa gaaaaattag
     2461 aaaaatttac agctagttac ggattaggct tagatagtgt tatattcaat atggatggaa
     2521 cgattgaatt aagattgcca agtggagaag tgataaaaaa gaatttatct gatctcatag
     2581 cgtaaggaat agcagtagaa aaagtctgaa tcaaaaatga agttctctca aaagttag
//
LOCUS       DAVLYV010000139         2622 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLYV010000139 DAVLYV010000000
VERSION     DAVLYV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.139"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..574
                     /locus_tag="VYO70_002081"
     CDS             <1..574
                     /locus_tag="VYO70_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1714230.1"
                     /translation="KIPSRLYKIDTGKVLFDGVNINQIDKKILSQNLGVVPQDSFLLN
                     RSILDNITLKHEVTSQKIEEVCKAVQIYDEIMAMPMKFNTIISEMGSNISGGQRQRIA
                     LARALINNPSIVILDEATSALDTINEERITKYIQSQGCTQIIVAHRLSTIKDADVIFV
                     MKGGKIVESGNHKYLMDLGGEYYSLYTKRK"
     gene            587..757
                     /locus_tag="VYO70_002082"
     CDS             587..757
                     /locus_tag="VYO70_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714231.1"
                     /translation="MKKENEYVILTTASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(1263..2609)
                     /gene="gdhA"
                     /locus_tag="VYO70_002083"
     CDS             complement(1263..2609)
                     /gene="gdhA"
                     /locus_tag="VYO70_002083"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1714232.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
BASE COUNT          845 a          453 c          507 g          817 t
ORIGIN      
        1 aaaaatacca tcaagattat ataagattga tacaggaaaa gttttatttg atggtgtaaa
       61 tattaatcaa atagataaaa aaatattaag tcaaaattta ggagtagttc cacaggattc
      121 atttttattg aaccgaagta ttcttgataa tataacttta aagcacgaag ttacttcaca
      181 aaagatagag gaagtttgta aagcagttca aatctatgat gaaatcatgg ctatgccgat
      241 gaaatttaat actatcatct cagagatggg gtcaaatatt tcaggtgggc aaaggcaacg
      301 gatagcactg gcacgtgcat taataaataa tcctagtatt gtaattttag atgaagcaac
      361 tagtgcatta gacactatta atgaggaaag aataacaaag tatatacaaa gtcagggctg
      421 tactcaaata attgtagctc atagattgtc aacgattaag gatgcggatg ttatttttgt
      481 aatgaaaggt ggtaagattg ttgagtcagg aaatcataag tacttaatgg atcttggtgg
      541 agagtactac agcttatata caaaaaggaa atgaggtgta aagaaaatga agaaagaaaa
      601 tgaatatgta attttaacaa cagcctcact aggggtgatg attggaatag tgtttgcaat
      661 ttttttagat tttccagttg aatatggtat ttctttaggc ttgttgaatg gaatagtatt
      721 gggttcgctg attgtttaca aaaacaataa gaattaagca taattttttg ctgtaaacta
      781 aggagtagag atggctatag ttgaaattat aaatctaaca aaaagcttta aagatattga
      841 agttattcat aacacttaaa taatagagca actacagtag tagcttaaaa acatgattaa
      901 atcgctattc ttaggagtag cggtttttct ttttgtttaa tactctttga aaatctcttc
      961 aaaccacgtc agctttgctt taccgtactc aagtacagcc tgcggctcgc ttcctagttt
     1021 gctctttgat tttcattgag tataaaaagg gtcaagtaag tatagtaaat tgaaataaga
     1081 tatgaacaaa tcgattagaa aagtcaaatt aatttctaga aatatgttag aaattggttt
     1141 gaattccgca atcaatttgt tcagttttta tttcatttca ttttatttaa ttagattttc
     1201 caatttttta attcaagcta aaaatcccca atcgtagtga ttgaggattg agtaaataaa
     1261 tcttaaacaa taccttgtgc aatcatggca tttgctacat tttcaaaggc agcaatgtta
     1321 gctcctgcaa ggtagtcttt atcaagaccg tatgtttctg aagtcgtttt agctgtgttg
     1381 aagatgtttg tcatgatgtc tttgagacgg ccatcaactt cttcacgagt ccatgagagg
     1441 cgaagactgt tttggctcat ttcaagagct gaaacggcta caccaccagc gttggcagct
     1501 tttgcaggtc cgtagaagat accattttct ttgtaaactt tgatggcatc aaggtcgctc
     1561 ggcatgttgg caccttcaga tacacagata acgccttgag caaccaaacg tttagctgct
     1621 tcaccgttga tttcgttttg agtggcacat ggaagagcaa tgtcatagtt tccagcgtaa
     1681 gtccatacag taccttcgtg gtaggttgca gttgcttttt cagctgcata ctcagtcaaa
     1741 cgagcacgac gtttttcttt aacatcaacc aaaagatcga agtcgatacc attttcatcg
     1801 atgacataac catttgagtc agaaacagaa ataacagttg caccgagttc agttgctttt
     1861 tgaagagcat attgagcaac gttaccagaa cctgaaataa cgactttctt accagcaaag
     1921 ctgttaccgt tagctttgag catttcttca gtatagtaaa ccaaaccgta accagttgct
     1981 tctggacgaa tcaagctacc accaaatcca agaggtttac cagtcaagac accagcatca
     2041 aattggttaa gacgtttgta ttgaccgtaa aggtaaccaa tttcacgtcc accaacaccg
     2101 atatcaccag caggtacgtc aagtgatggt ccgatgtgtt tttgcaattc agtcatgaag
     2161 ctttggcaga agcgcatcac ttcagcatct gttttacctt taggatcgaa gtctgatcca
     2221 cctttacctc caccgatagg aagtccagtc aagacatttt taaagatttg ttcaaatccg
     2281 aggaatttca agatcccttg gtttacagtt gggtggaaac gaagtccacc tttgtatggt
     2341 ccaacagctg agttgaattg aacacggtaa ccacggttta cttgaatttt tccatcacgg
     2401 tcaacccaag gaacacggaa agaaaccacg cgctcaggct cagtaatacg tgccaagata
     2461 ttttcttcga tatactcagg gtgtttttca aatacaggtt ctaaagtgtt gaaaaattct
     2521 tcaacagctt ggaggaattc agcctcgtgc ccgttacgag ctttcacagt ttcaaacacg
     2581 ctttggatat attctttagc agatgtcata tcgttctcct tt
//
LOCUS       DAVLYV010000140         2562 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLYV010000140 DAVLYV010000000
VERSION     DAVLYV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.140"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            226..2544
                     /locus_tag="VYO70_002084"
     CDS             226..2544
                     /locus_tag="VYO70_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV1714233.1"
                     /translation="MRNGKLVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYI
                     LSDKRWDEAPIYLRYGVFRPSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPD
                     FLNKWLDKKDKKQGDFPFIIDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFD
                     GTYSSERLASECKALKILNEWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFP
                     LYEVVDKTKDYLLRVSKIVEKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWE
                     QAVFSNDEKVVHKVAAPGFRAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVS
                     QTRIEGKKLFESLGYTREHIDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESE
                     QDIQDFIIKLLRGFTLTYGQWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLA
                     KKVKNFKFDDYYEIRTQVINEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELF
                     TTHFIEIFENSQNKNLYSGQAGILLVGLYFLSKGGIDNKLGEEILIRLREYTLNYIEN
                     PETFCKVGASDVQSNDPYENFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKE
                     LVAYKVDSNNSLQYSQGHRLLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATD
                     VVFCVLPGLFNGFCGLEVANNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNG
                     LKITTDIASGFAGVAIGLVSIMDNKLTILPQI"
BASE COUNT          907 a          312 c          526 g          817 t
ORIGIN      
        1 agcataaagt accaagtaag tctgcctttt aattggcacg aagtaatgaa taatgatgag
       61 tgggtttatc aataccctat tggaaaattt gttgaaagac aagggtggaa aatacacatt
      121 agttcagagt ataacagttc acatgaattg ttacaagatg tagcaaaaat ttgccatgaa
      181 atgcgaattc catttaaaca tcttagtaca gaagacaaat ttatcatgcg aaacgggaaa
      241 ttggttagtc gaggattttc tggtaaattt attacatgtt atccaaatca aaatgaatta
      301 gagtcggttt tgcaaaggtt agaaagtgct ttaaagcaat ataatgggcc atatatttta
      361 agcgataaac gttgggatga agcaccaata tatctgaggt atggtgtgtt taggccgtca
      421 agggatgatg aaaagaaggt tgctattgat gaattaattg ttggcgatga agttgttaaa
      481 gatgaacgtt taccagtatt taaaattcca aaaggtatag tgccccctga ctttttgaat
      541 aaatggcttg acaagaaaga taaaaaacag ggagactttc cctttattat agataatgca
      601 attagatttt caaacagtgg tgggatatat aatgctaggc taaaagaaga tgggaaaaaa
      661 ataattttaa aagaggctag accgtataca ggtctaggat ttgatggtac ttattcttca
      721 gagagattag cttcggagtg caaagcacta aaaattctaa atgaatggag tgagacgcct
      781 aaaatatact ggcacggtaa gatctgggag cacaccttct taggaattga acatatgaag
      841 ggcgttccac taaatagatg ggttaccaat aattttcctt tatatgaagt tgttgataag
      901 accaaagatt atctattgag agtttcaaag atagtagaaa aactcatcga tttaacgaat
      961 aagtttcatt ctgaaaatgt atatcatcaa gatttacatt taggaaatat cttggttaag
     1021 gatgaagatg agattagtat aatagactgg gaacaggctg tcttttcaaa tgatgaaaaa
     1081 gttgtacata aagttgcagc ccctggtttt agagcatggc gagaaacact tcccagtgag
     1141 atagattggt atggtattag acaaatagct cattatttat acatgccact agtaactaca
     1201 tcggatttga catacaacta tgtaagtcaa accaggatag aaggaaaaaa actatttgag
     1261 tctttgggtt atacaagaga acatatagat tatgtagaaa gtttattgtc atatctagat
     1321 tctaaatgtc cacagattga gaatatttcc aggaaaaaag ttttaaaacc catgcatgag
     1381 attagaacta tcgagagtga gcaagatata caagatttta ttataaaatt attgagaggg
     1441 tttacgctaa cttatggaca gtggcgtaaa gaatttcaat caagattctt cccagttcat
     1501 tattatggtc tgaattttaa tcaaggaatt gcgttttcag atttagctat cctttggagt
     1561 tatcaacaac tagctaagaa agtaaaaaat ttcaagtttg atgactatta tgagattcga
     1621 acacaggtta ttaatgaggc agtaaataat tttaagaaat cgtccctttc tggtcttttt
     1681 gatggtaaaa ttggtacaat ctggctcatt tatgaatttg gggagataga tcgggcagtt
     1741 gaacttttta caactcattt tatagagata tttgagaatt cacaaaataa aaatttatat
     1801 agtgggcagg ctggaatttt actggttgga ctgtattttc ttagtaaagg gggaattgat
     1861 aataaactag gagaagaaat actaattagg cttagagaat atactttaaa ttatatagaa
     1921 aatccagaaa ctttttgtaa agttggcgct agtgatgttc agagtaacga tccttatgag
     1981 aattttggtg gattactata tggtcacgca ggggtagctt ggctcttcgg tgaagcttat
     2041 aaattaactg gtgaaagtat ctataaaaat ggtctagaat tggctgtaga taaagaatta
     2101 gttgcttata aagtagatag taataattca ttgcagtaca gccaaggtca tagattacta
     2161 ccatatctag caactggaag tgctggcctg cttttactta ttaatagaaa taaagaaatc
     2221 ttgtcaagca aatacttaaa atatttaact tctttagaga gggcaactga tgtagttttt
     2281 tgtgtattac caggtttatt taacggattt tgtggtcttg aagttgctaa taatatatat
     2341 agtgatatcg atgataactt ttcaggacaa aagaaattaa ttgaacaact ttatagatat
     2401 ttgtgtgtaa ttgaagaagg atttgttatt gcgggagata atggtttaaa gataacaaca
     2461 gatattgctt cgggatttgc gggtgttgca attggattgg tatcaataat ggataataaa
     2521 ttgacgattc ttcctcaaat ataatattta gataccatta tt
//
LOCUS       DAVLYV010000141         2438 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLYV010000141 DAVLYV010000000
VERSION     DAVLYV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.141"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            114..536
                     /locus_tag="VYO70_002085"
                     /pseudo
     CDS             114..536
                     /locus_tag="VYO70_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211071.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(748..1140)
                     /gene="rpsI"
                     /locus_tag="VYO70_002086"
     CDS             complement(748..1140)
                     /gene="rpsI"
                     /locus_tag="VYO70_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1714234.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(1159..1605)
                     /gene="rplM"
                     /locus_tag="VYO70_002087"
     CDS             complement(1159..1605)
                     /gene="rplM"
                     /locus_tag="VYO70_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1714235.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            1962..2279
                     /locus_tag="VYO70_002088"
     CDS             1962..2279
                     /locus_tag="VYO70_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1714236.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
BASE COUNT          748 a          473 c          444 g          773 t
ORIGIN      
        1 aagaaaatta agataaaact aaaaggactt agtcctgtgc aatacagaac taaatccttc
       61 ggataaatta attgtctaac tttttggggg cagtacataa gaaccttggt aatatgcgtt
      121 ttattatggc aacatttccc attttctcta tttctaatga cttacttcaa ctccttacct
      181 cttggaaaga acaacaaaga tatgaactag caaagtttgg catcctcagt aatccagaac
      241 aatttgtttt tacatatatc gacacaaaag gaaacatcaa caagccttta catgccgact
      301 atctgaacaa taaaatgaaa agcattagaa agcgacataa agaactgaca cacgctacac
      361 ctcacaaatt acgccataca ggcgcaactc tagctaaata agcaggaatg agcttagaag
      421 ccatttctga agcactaacg catagcgata caggcacaac tcaaatttat gtcaacacct
      481 ctaatgtagt tcctatgaca gtaggtgaat ttgctttaaa gtctctaaaa caataaggta
      541 tagtagattg aaactagaat agtacacctc tacttctaaa acattgttag aattcgattt
      601 gactgtcctg atcgatttgt cctattctta tttcatttcg ctataaaaat aaggtaaact
      661 ttgagataaa cttttcaaaa aaacacgaaa aaagcaccca taaggtaaac ttttgagtgc
      721 tttgaaacgt tgatataatc gtattgatta acgtttacta aattgtgatg ctttacgagc
      781 tttcttaaga cctggtttct tacgttcaac tttacgtgag tcacgtgtaa gaagtcctgc
      841 gcgtttcaat gaatcgcgga agtctgggtc tacttgaaga agggcacgag cgataccgtg
      901 acggatagct cctgattgac cagcgtatcc accacctaca acgttaacga aaacgtcgta
      961 tgaacctaca gttgaagtaa ctgcgaatgg ttggttgatg acaagacgaa ggtcagcgtg
     1021 tgggatgtac tcttcaacat cttttttgtt aacagtgatt ttaccagttc ctggaacaag
     1081 gcgaacgcgt gcaacagcgt ttttacgacg tccagtacct gcatattgtg cttgtgacat
     1141 actttattgt tcctttcctt agataagtcc tgaaatgtca agaacttctg gttgttgtgc
     1201 agcgtgagtg tgctcagctc caacaaatac tttcaacttc ataccttgag cgcgtccaag
     1261 tgtattgtgt ggaagcatac ctttaactga tttctcaatc aaacgtactg catttttaga
     1321 acgaagttca cctgcagaga tttgtttcaa tccacctggg tggtttgagt gagtgtagta
     1381 gattttatca gttgcttttt taccagtcaa tttaactttt tcagcattga taacaatcac
     1441 gaagtcacct gtatcagtgt gtggtgtaaa tgttggtttg ttttttccgc gaagtacgct
     1501 agcaactact gcagaaagac gtccaagtgg tacatcagtt gcgtcaacta cgtaccattt
     1561 acgttcaact tggcctggtt tagccataaa tgttgttttg ttcatgattt ctcctatata
     1621 tagttcgatt tttgtttacg gtgatgggtg ttccttccca tcgaaaagta tttggaaggt
     1681 tccggggcct ttcaaatggg gtaaacaata ccgcctacta tcataccaaa tttcacccct
     1741 aaaagtcaat agatatgaaa aatatttttc ggtaacttct caaagtaact gaaagttata
     1801 taatcaagtt aatttgggaa tttacctatt aacttcggta gatttttgag aatttttttc
     1861 cttctttttc cttgtagtta aaatcctttt caaaaaatca ccttttcttg atttttaatc
     1921 ctatttgctg tatgataagg gaaaagaaag gggacagaga tatggctttt accaataccc
     1981 acatgcgatc tgctagtttt ggcattgtta ccagcttgcc tgatgacatc attgactctt
     2041 tttggtatat tatcgaccat ttcttaaaaa atgtctttga attggaagaa gaactcgagt
     2101 ttcaattgct taataaccaa ggaaagatta ccttccactt ttcaagtcaa cacctcccta
     2161 cagccattga ttttgacttt aaccatcctt tcgaccctcg ttatccccca agagtactgg
     2221 ttttagacat ggacggtaga gaaactatcc tcctcccaga agaaaatgac ctattttaat
     2281 acttaatgaa aatcaaagag caaactagga agctagccgc aagctgtact tgagtacggt
     2341 aaggcgacgc tgacgtggtt tgaagagatt ttcgaagagt ataaaaactc tagccttcag
     2401 ttgcaagtaa ctgaaaacta gagtttttct attttttc
//
LOCUS       DAVLYV010000142         2403 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLYV010000142 DAVLYV010000000
VERSION     DAVLYV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.142"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..324)
                     /locus_tag="VYO70_002089"
     CDS             complement(<1..324)
                     /locus_tag="VYO70_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1714237.1"
                     /translation="MAYSIDFRKKVLSYCERTGSIAEASHVFQISRNTIYGWLKLKEK
                     TGELNHQVKGTKPRKVDRDRLKNYLTDNPDAYLTEIASEFGCHPTTIHYALKAMGYTR
                     KKEPHL"
     gene            401..842
                     /locus_tag="VYO70_002090"
                     /pseudo
     CDS             401..842
                     /locus_tag="VYO70_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013315450.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            1007..1306
                     /locus_tag="VYO70_002091"
     CDS             1007..1306
                     /locus_tag="VYO70_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1714238.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            1567..1890
                     /gene="tnpB"
                     /locus_tag="VYO70_002092"
                     /pseudo
     CDS             1567..1890
                     /gene="tnpB"
                     /locus_tag="VYO70_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2027..>2403)
                     /locus_tag="VYO70_002093"
     CDS             complement(2027..>2403)
                     /locus_tag="VYO70_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1714239.1"
                     /translation="TGESFFLRAGGCNTEWMNVFLEELSQAYPYDYFLLVMDNAIWHK
                     SSTLKIPTNIGFAFIPPYTPEMNPIEQVWKEIRKRGFKNKAFRTLEDVMNQLQDVIQG
                     LEKEVIKSIVNRRWTRMLFESR"
BASE COUNT          769 a          465 c          422 g          747 t
ORIGIN      
        1 taggtgtggt tctttttttc gagtgtagcc catcgctttg agcgcatagt ggatggtagt
       61 tggatgacag ccaaattcag aagctatttc agtcaaataa gcgtctggat tgtcagtaag
      121 atagttttta agtctatctc tatcaacttt tcttggtttt gttcctttta cttggtggtt
      181 tagctctcct gttttctctt ttagctttaa ccagccataa atggtattac gtgagatttg
      241 gaaaacgtgt gatgcttctg ctatactacc tgttcgctca caataagaga gaactttttt
      301 acgaaaatct attgaatatg ccataagaag attataccac attgtgtact atttttggtt
      361 catttcacta taataataac gatactagat aatgaggaaa atgcaatggc aaacccaact
      421 ttcggagaaa aaagcgaata cagactatat tgctcgctat ggcgtatatg cagttatccc
      481 taatcctgaa caaaaacaaa ttgttcttgt tcaagaacca aatggtgctt ggttcctacc
      541 atgtggagaa attgaagcag gtgaaaatca tcaggaagcc ctaaagcgtg agttgattga
      601 agagcttggt ttcacagcag aaattggtac ctattacgga caagctgacg aatatttcta
      661 ttctcgtcat cgtgatacct actactccaa tcctgcctac ctctatgaag caactcctaa
      721 agccactaga aaactttaat catattgcct ggttccctat tgacgaggct atcaaaaacc
      781 ttaaacgtgg tagccataaa tgggccattg aatcttggaa aaaacagcat aagattgact
      841 aatactcttc gaaaataaaa ttcaaaccac gtcagcgtcg ccttaccgta ctcaagtata
      901 gcctgcggct agcttcctag tttgctcttt tattttcatt gagtataaat caagcgactt
      961 tattcaaaaa gtgctataat agaattagaa aatcggagga aatattatga agcttatcaa
     1021 tacgacaaac tcacactcgc aacttgtaaa aagtcagcta gaaagtaccg atgcaaccct
     1081 tgttgaggtt tattctgcag ggaatacaga tgttattttt acccaagctc cgcttcacta
     1141 tgaaatcctc atctcaaaca aacaccgtgc tattcgtgag cctgaaatcg aaaccattca
     1201 agaatttttc ttgaaacgta aaattgataa gacctctgtt gatgaagcca atatcaagac
     1261 actctactca gagaaattaa ttggaatttc aatcccaatc aagtaagaaa attgagtaaa
     1321 aactcgattt gagagaaagt ccaataagtc tttccataac aaaacgcata gtaacaagag
     1381 tttctacacc cactattata cgtttttatc attttgaaat ctgctgctaa tcaatgaaga
     1441 agcaaagatc aaactaggca gctagttaca agttacgtaa gcgcgtcata acaaggtatc
     1501 tatcctaatt ccccaacttc cagtatacta gataaaaaat aattatcaga ggaaaagtat
     1561 tgtactatga tgattctact aagtgattta ggtcaagttc accttgtttg tggcaagaca
     1621 gatatgaggc agggaataga ctcattagcc tatgtagtta aaacccactt tgaattggat
     1681 cctttctcag gtcaagtttt tctcttttgt ggtggacgta aagaccgctt taaagccctt
     1741 tactgggatg gtcaaggatt ttggctacta tataaacgct ttgagaacgg aaaactgact
     1801 tgaccaagta cagaaaagga tgtcaaagct ctcgcacctg aacaagtaga ttggcttatg
     1861 aagggctttt ctatcactcc aaaaatatag tagattgaaa ctagaatagt acacctctgc
     1921 ttctaaaaca ttgttagaaa tcgatttgtc ctgttcttat ttcattttac tataaatcca
     1981 tcagaaagtc gtgatttcta ttgaaatgag gactttcttt ttatactcat ctgctttcaa
     2041 aaagcattct agtccatctc cgattaacga tggactttat cacctccttc tccagtcctt
     2101 gtatgacatc ttggagttga ttcatgacat cttccaaagt tcgaaaggcc ttattcttaa
     2161 atccacgttt acgaatctct ttccacactt gttcaatggg gttcatctct ggtgtgtatg
     2221 gaggaataaa tgcaaagcca atattagtcg gaatctttaa ggtacttgat ttatgccata
     2281 tagcattgtc cataacgagt aaaaaataat catatggata agcttgtgaa agctcttcta
     2341 aaaagacgtt catccactca gtattacatc caccagctct taagaaaaat gattcgcctg
     2401 tat
//
LOCUS       DAVLYV010000143         2369 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLYV010000143 DAVLYV010000000
VERSION     DAVLYV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.143"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..125
                     /locus_tag="VYO70_002094"
     CDS             <1..125
                     /locus_tag="VYO70_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1714240.1"
                     /translation="LYKRFENGRLIWLSTEKDVKALTPEQVDWLMKGFSITPKI"
     gene            complement(280..588)
                     /locus_tag="VYO70_002095"
                     /pseudo
     CDS             complement(280..588)
                     /locus_tag="VYO70_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            579..1019
                     /locus_tag="VYO70_002096"
     CDS             579..1019
                     /locus_tag="VYO70_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1714241.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNWHIKASQYYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHDQCRSGSVVQEFLGDYSGYVHCDILRQ"
     gene            1114..1543
                     /locus_tag="VYO70_002097"
                     /pseudo
     CDS             1114..1543
                     /locus_tag="VYO70_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295150.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1614..1772
                     /locus_tag="VYO70_002098"
     CDS             1614..1772
                     /locus_tag="VYO70_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV1714242.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVLEAYLPWT
                     KVVQEKCK"
     gene            1865..2369
                     /locus_tag="VYO70_002099"
                     /pseudo
     CDS             1865..2369
                     /locus_tag="VYO70_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          733 a          444 c          487 g          705 t
ORIGIN      
        1 tactatataa acgctttgag aacggcagat tgatttggct aagtacagaa aaggatgtca
       61 aagctctcac accagaacaa gtagactggc ttatgaaggg cttttctatc actccaaaaa
      121 tatagtagat tgaaactaga atagtacacc tctgcttcta aaacattgtt agaaatcgat
      181 tttactgtcc tgatcgattt gtcctgttct tatttcattt tactataaat ccatcagaaa
      241 gtcgtgattt ctattgaaat gaggactttc tttttatact catctgcttt caaaaagcat
      301 tctagtccat ctccgattaa cgatggactt tatcacctcc ttctccagtc cttgtataac
      361 atcttggagt tgattcatga catcttccaa agtttaaaag gctttattct taaatccacg
      421 tttacgaatc tctttccaca cttgttcaat ggggttcatc tctggtgtgt atggaggaat
      481 aaatgcaaag ccaatattag tcggaatctt taaggtactt gatttatgcc atatagcatt
      541 gtccataacg agtaaaagat aatcatctgg ataagcttgt gaaatctcct attcctaaag
      601 cccctttagc gcataacttt ggctcagctt ctattatcgc tcacaccatc catcagaagt
      661 ttaatctgaa ggtacccaat tatcgccaag aagaagattg ggctaggatg ggtttaccaa
      721 tcacacgtaa ggaaatctct aattggcata tcaaggcgag tcaatactat ttggagcccc
      781 tttataacct cttgcgagag agactattga ctcagccctt acttcatgcg gatgaaactt
      841 cttatagggt gctagagagt gatagtcagc tgacttacta ttggactttt ttgtcaggta
      901 aagcagagaa acaagggatt acgctttacc accatgatca gtgtcgaagt ggttcagtag
      961 tacaagaatt cctaggagat tattctggct atgtgcattg tgatattttg cggcagtaac
     1021 ttaggacttt agtcctctag ttctgcctat gcgatagcag tccaaggttt aggagcaagg
     1081 cgacgctaag cttggtaaac ttcgaaccgc tcgtctgctt atcgtcaact ggaagaagct
     1141 gaacttgttg gatgttgggc gcatgtgaga aggaagtttt ttgaagcgcc cccccaagca
     1201 agcggataaa tcatccttag gagctaaagg tttagcttat tgtgatcagt tattttcctt
     1261 ggaaagagac tgggaggctt tgccagctga tgaacgacta cagaaacgtc aagaacatct
     1321 ccagccctta atggaagact tctttgctta gtgccggcgt cagtcagttt tagcaggttc
     1381 aaaactagga agggcaattg aatacagcct caagtatgaa gaaaccttta agaccatttt
     1441 gaaagacgga catctggtcc tttccaataa tctagctgaa cgcgccatta aatcattggt
     1501 tatgggacgg agtaaaagag tccagtggac tcttttagcc taagctcagt ttaaaaaagc
     1561 gagggtggtt attttctcaa agttttgaag gagctaaagc aagagctatt attatgagtt
     1621 tgttggaaac agctaaacgt catcaattaa atagcgagaa atatctatcc tatcttctag
     1681 aatgtcttcc aaacgaggaa actctcgtaa acaaagaggt tttagaggct tatttaccat
     1741 ggactaaagt tgtacaagaa aagtgcaaat aagaaatctc cagattagga actatccgtg
     1801 agttctccag tctggagatt tttcaataga cttcctgcga aacaaaatat ggtataatag
     1861 ttctatgaat gatgaagcaa gtaaacaact aaccgatgca cgatttaagc gtcttgttgg
     1921 tgttcaacgc acgacttttg aagagatgtt agctgtatta aaaacagctt atcaacttaa
     1981 acacgcaaaa ggtggacgaa aacctaaatt aagtctagaa gaccttctta tggccactct
     2041 tcaatatgtg cgagaatatc gaacttatga acaaattgcg gctgtttttg gtattcacga
     2101 aagcaactta atccgtcgga gccaatgggt tgaagtaact cttgttcaaa gtggtgttac
     2161 gatttcaaga actcctctca gttctgagga cacggtaatg attgatgcga cggaagtaaa
     2221 aatcaatcgc cctaaaaaaa gaattagcga attattctgg taaaaagaaa tttcacgcta
     2281 tgaaggctca agcgattgtc acaagtcaag ggagaattgt ttctttggat atcactgtga
     2341 actattgtca tgatatgaag ttgttcaaa
//
LOCUS       DAVLYV010000144         2307 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLYV010000144 DAVLYV010000000
VERSION     DAVLYV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2307
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.144"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..1074
                     /locus_tag="VYO70_002100"
     CDS             <1..1074
                     /locus_tag="VYO70_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714243.1"
                     /translation="IRLNPDSIISECYSKKDLIHETTNGTTDYHPLDLYKTPQERVDL
                     NFSLWKERRKFISASNIKSVKIAVFYYSHDSLMNHDGSLKKVSGLIQVPMNRADDNQY
                     IADLKYLIEEEIKWLIGPVSIFLKYAKICKKYCIKFNVEYCECTSEYVPEVYRKEIED
                     VFKCKFLMQYSCHELWGIAFTNSLEMYNVLKPLDNSIVEGLLRKEFTSKKLYSPVVTS
                     LTLKAMPFIKYLLTDLVYFDNGYIIPFGFRYTDMVKLKTDTIHCSIFDNLFGTLPFKF
                     EPLEQYQIIYDAQNSEIVVNVLNLEVTQGDIVCSVLKEYIKEHYKSSIKVSLRNCSKF
                     FRDNLTGKMRGIINKENINNEIW"
     gene            1090..>2307
                     /locus_tag="VYO70_002101"
     CDS             1090..>2307
                     /locus_tag="VYO70_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEV1714244.1"
                     /translation="MDNENEISKFFLSSENVKDMKTIANDILNMGLDFKLSEKVSQKK
                     MKEHDDLNFLSLEESIDNLPQLLEEFREKVIPYCTNFSSPNFMGFPDSGNSLGGLSGS
                     LLSDLLQQNLINASFCAPVATIMEINVIQWLRKVLGYSTSDVHNIMEVGGIVTYGGTG
                     SNSTAMLLARENKDGNTLELGVRNPRNYKVIVPKGIGHYSIASSLKWLGLGNNIIEVP
                     TENYRYNLKKLREALINHSNEIMAVVAYAGDSRTMTIDNLSEVADLVKTLTPNVWLHC
                     DACHGFSLAFSKKYKDRIKGIEKFDSISTDPHKVLGIPYCISVLLVKTPQSLNLIHSQ
                     SDLIMNENLALGQTTPFLGSKSWISLKLWLVAKHLGSEKIGAMVDKRIELTNYLQDLI
                     LKSSKLILLNRTDI"
BASE COUNT          840 a          292 c          420 g          755 t
ORIGIN      
        1 attagattaa acccagattc tattatttca gaatgttaca gcaaaaaaga tcttatacat
       61 gaaacaacta atggcacgac agattatcat ccattggatt tatacaagac tcctcaagaa
      121 agagttgact tgaacttttc actttggaaa gaaagaagaa aatttattag tgcttctaat
      181 attaagtctg ttaagatagc agttttttat tacagtcatg attcattaat gaatcatgat
      241 ggcagtctga aaaaagtatc gggattgatt caagttccaa tgaatagggc tgacgataat
      301 caatatattg cggatttaaa atatcttatt gaggaagaaa taaaatggct gattggtccg
      361 gtttcgatat tcttgaaata tgcaaagatt tgtaaaaaat attgtattaa atttaatgtt
      421 gaatattgtg aatgtactag tgagtatgtc cctgaagtat atagaaagga aatagaagat
      481 gtatttaaat gtaaattttt aatgcaatat agctgtcatg agttatgggg aattgcattt
      541 acaaattcat tagaaatgta taatgtactt aaaccattag ataattctat agttgaaggt
      601 ctgctgagaa aagaatttac ttctaaaaaa ttatatagtc cagttgttac ttcactaact
      661 ctaaaggcaa tgccatttat aaaatattta cttactgatt tggtttactt tgataatggg
      721 tatattatcc cgtttggctt tagatacaca gacatggtaa aactaaaaac agataccatc
      781 cattgttcaa tatttgataa tttatttgga acattaccat ttaaatttga acctttagaa
      841 cagtatcaga ttatctacga tgctcaaaat tcagagattg tggttaatgt tctaaattta
      901 gaagtgacac aaggtgatat tgtttgctcg gttttaaaag aatatatcaa agaacactac
      961 aaaagttcaa taaaagtttc tcttcgaaat tgttcaaaat tttttagaga taatctgaca
     1021 ggaaaaatgc gtgggataat aaataaagag aatataaata atgaaatttg gtaggaggca
     1081 aactcataca tggataatga aaacgaaatt tcaaagtttt ttctgagttc agaaaatgta
     1141 aaagatatga aaactatcgc aaatgacatt ttaaatatgg ggttggattt taaattaagc
     1201 gaaaaagttt ctcagaaaaa aatgaaagaa cacgatgatt taaattttct cagcttagaa
     1261 gagagtatag acaatcttcc gcaacttttg gaagaattca gggaaaaagt aattccttac
     1321 tgtacaaatt tttcttcccc taattttatg ggttttcctg actcaggaaa ttcattaggc
     1381 ggtctttcag gtagtttgct tagtgattta ttacagcaaa atttgattaa tgcatctttt
     1441 tgcgctcccg ttgctactat tatggaaata aatgtaatcc agtggttacg aaaagtttta
     1501 ggatattcca cttctgatgt tcataatatt atggaagtag gcggcatcgt tacctatggt
     1561 ggtacaggaa gtaattctac agctatgctt ttagcaagag aaaataaaga tggaaatact
     1621 cttgaattag gcgtaagaaa ccccagaaac tataaggtaa ttgtgccaaa aggaattggt
     1681 cattatagta tcgcatcaag tttaaagtgg ctgggattgg ggaataatat tattgaagtt
     1741 cctacagaaa attatcgcta taacttgaag aaacttaggg aagcactaat caaccattct
     1801 aatgaaataa tggcagtggt tgcatatgct ggagatagta gaacaatgac tattgataat
     1861 ctatctgaag tagcagattt ggtaaagaca cttaccccaa atgtttggtt acactgtgat
     1921 gcttgtcacg gattttcatt agctttttct aaaaagtata aagataggat aaaaggaatt
     1981 gaaaaatttg atagtatttc gacagatccg cataaagttt taggaattcc ttattgcata
     2041 agtgttttgt tagtgaaaac acctcaatcg cttaatctaa ttcactctca atcagatttg
     2101 attatgaatg aaaacttggc ccttgggcaa acgactccat ttttgggaag taaaagttgg
     2161 atatctctaa aattgtggtt agtagctaaa catttaggat cagagaaaat aggagcgatg
     2221 gtagacaaga gaatagagtt aacgaattat ttgcaagatc taatacttaa gagttctaag
     2281 ttaatattat taaataggac agatatt
//
LOCUS       DAVLYV010000145         2235 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLYV010000145 DAVLYV010000000
VERSION     DAVLYV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.145"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(425..1192)
                     /locus_tag="VYO70_002102"
     CDS             complement(425..1192)
                     /locus_tag="VYO70_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA helicase"
                     /protein_id="HEV1714245.1"
                     /translation="MDNKRLIHAVAGSGKTTKIIETIDPQKRNLILTYTETNQNTIRA
                     KLIEKFGYIPESTFIFGVFESLYSFCLVPYLGKRPKGINFDYKTQGKFDKTAIDNTGR
                     IVQNQLSKSLLRGSLIYKRKEIRFDNSYLDRIDKFFDCIYVDECQDFESDDFDWLLSL
                     SNLNAEVFLFGDYYQKTFSTSRRGNKGKRIHSSFENWLKVISDSGYEIDLSSLSKSYR
                     CPKIVCDFIVDNLAIEILSQREEKLSSQITLIDSQKK"
     gene            complement(1174..>2235)
                     /locus_tag="VYO70_002103"
     CDS             complement(1174..>2235)
                     /locus_tag="VYO70_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1714246.1"
                     /translation="IDTQQEIQKRIHEVYLDNISAENRAKVNHQYRINSFNFLKTLRK
                     DELLKENASEFQLHFDESENSFRNSVSAVKNGVDIKNLGQGEKVLLGVENAYSHLKET
                     VKILLIEEPENHLSFQNLQKLVRMLSSNTGVQVFIGTHSNMIASRLGVDNLLFLDNGQ
                     ISKLKGLKKDTVRFFKKSTNQNLLNFSLGKKIILVEGNAEYILVEKFFEILHSKKPEE
                     FNVSIISVDGLSFERYLEIATNFTDKKVVVITDNDGDYESKVQSKYSSYQQFQNIKVS
                     SDLDNDNRTFEICIYNCNSDLLESSGLTKSNDLLEYMLREKAEFALRLLEQMETDNIR
                     EKFIIPNYIKEAIEWIIKD"
BASE COUNT          742 a          397 c          262 g          834 t
ORIGIN      
        1 gcaacaagac ttgtggaata ttactgataa agcaaataag aattggaatc tccaaaaaaa
       61 ttgatataat atactgccat ttcattggtc tttttataaa taaaatttta actattctca
      121 tattctctcc taatttgata tttcccttac atcatcttcc tatatttttc tatatcggat
      181 tgctttacaa aatataagtt ccccctcgta cgtgtacacg caacgtaaaa tttacttttg
      241 gtttgtgaag aaagttcatt caagagatca gctgcgaata atttgtatga tgttggattt
      301 aatactacac aaacattgtc atattcagag cctttactat ccccccaatt ctgactcttg
      361 cattcataat cataagattt ttgataaaaa agtttcataa tattgtcatc tgtcattatt
      421 gattctattt tttctgtgaa tcgataagag taatttggga agatagcttt tcttctcgct
      481 gtgataaaat ttcaatagct aaattatcaa caataaagtc acaaactatt ttgggacaac
      541 gataactttt tgacaaactc gataaatcaa tttcatatcc tgaatcagaa ataactttta
      601 accaattctc aaaactcgaa tggatccttt ttcctttatt acctcgcctt gaagtactaa
      661 aggttttttg ataataatct ccaaataaaa atacttctgc attaagatta cttaaactta
      721 aaagccaatc aaaatcatct gactcaaaat cctgacactc atctacataa atacaatcaa
      781 aaaatttatc aattctatcc aaatatgaat tatcaaatct aatttctttc ctcttataga
      841 ttaatgaacc ccttaataga gatttagaaa gttgattctg aacaattctt ccagtattat
      901 ctatagcagt cttatcaaat tttccttgag ttttataatc aaaatttatt ccttttggtc
      961 gtttccctaa atatggcact aagcaaaaac tataaagaga ctcaaaaaca ccaaaaataa
     1021 aagtagactc aggaatgtag ccaaattttt caatcaactt tgctctgatt gtattctggt
     1081 tcgtctctgt atacgtaagg ataagatttc ttttttgagg atcgatggtt tcaattattt
     1141 tagttgtttt gccacttcca gcaacagcat gaatcaatct tttattatcc attcaatcgc
     1201 ctctttaata taattgggta taataaattt ttcccttata ttatcagttt ccatttgttc
     1261 tagtaatctc agtgcaaatt cagccttttc cctgagcatg tattctaata aatcattaga
     1321 tttggttaat cctgaagact ctaataaatc cgagttacaa ttataaatgc atatctcaaa
     1381 tgttcgatta tcattatcta aatcagaaga aacttttata ttctggaatt gttgataaga
     1441 actatatttt gactgtactt tactttcgta atcaccatca ttatccgtga taactactac
     1501 ttttttatca gtaaagttag ttgcaatttc taaatacctc tcaaaactta atccatctac
     1561 agaaataatt gatacattaa attcttcagg ctttttggag tggagtattt cgaaaaactt
     1621 ctctactaaa atatactcag cattcccctc aacaagaatt atcttcttac ctagagagaa
     1681 gttcaataaa ttttgattag tagatttctt gaaaaatctt acagtatctt ttttcaaacc
     1741 tttaagttta gatatctgac cattatctaa aaaaagaaga ttgtctacac ccaatcttga
     1801 agcaatcata tttgaatgag tcccaataaa aacttgaaca cctgtatttg agcttagcat
     1861 tctaaccaat ttctggaggt tttggaagct caaatgattc tcaggttcct caataagtag
     1921 tattttaacc gtttccttga gatgactgta tgcattttca acacctaata ataccttttc
     1981 tccttgccct aaatttttta tatctacacc attttttaca gctgatactg aatttctaaa
     2041 tgaattttct gattcgtcaa aatgaagttg gaattctgaa gcattctcct tcaacagttc
     2101 atccttcctt aaagtcttta aaaaattaaa tgaatttatc ctgtattgat gattaacttt
     2161 tgctctattc tcagctgata tattatccaa atatacctca tgaatacgtt tttgaatttc
     2221 ttgttgtgta tcaat
//
LOCUS       DAVLYV010000146         2170 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLYV010000146 DAVLYV010000000
VERSION     DAVLYV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.146"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            172..648
                     /locus_tag="VYO70_002104"
     CDS             172..648
                     /locus_tag="VYO70_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1714247.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            648..1406
                     /locus_tag="VYO70_002105"
     CDS             648..1406
                     /locus_tag="VYO70_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1714248.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYAINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTT
                     SAATVLHDLLFGELNQVEKEMLKSGEERLKDLTN"
     gene            complement(1804..>2170)
                     /locus_tag="VYO70_002106"
     CDS             complement(1804..>2170)
                     /locus_tag="VYO70_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1714249.1"
                     /translation="LGDQTTFNILETYINKTGKESNDFLFPGKHLPKPLSEQSVRLIL
                     KRIVEQNSLSKTITPHMFRHSFATMLLDNDVDIRYIQQILGHSSISVTQIYTHVSHSK
                     QKEILSSFNPMSVIHSEIE"
BASE COUNT          770 a          289 c          404 g          707 t
ORIGIN      
        1 agatatcgtt tgcgcatcga aaaaattaat acaagaataa atatttatag ctctttaggt
       61 gacttttata gaagtaaagt ttaggataga aaaacaagaa ataacgcacc atttttggtg
      121 cgttatgctt ttttatgcta taatggattt ataaaaataa aggagtttgc tatgattgga
      181 aagaacataa aatccttacg taaaacacat gacttaacac aacccgaatt tgcaaggatt
      241 gtaggtattt cacgaaatag tctgagtcgt tatgaaaatg gaacgagttc agtctctacc
      301 gaattaatag acatcatttg tcagaagttt aatgtatctt atgtcgatat cgtaggagaa
      361 gataaaatgc tcaatcctgt tgaagattat gaattgactt tgaaaattga aattgtgaaa
      421 gaaagaggtg ctaatctatt atctcgactc tatcgttatc aagatagtca gggaattagt
      481 attgatgatg aatctaatcc ttggatttta atgagtgatg atctatctga cttgattcag
      541 acaaatatct atttagtaga aaattttgat gaaatagaga gatatagcgg ctatttggat
      601 ggaattgaac gtatgttaga gatatctgaa aagcggatgg tagcttaatg gaaatccaag
      661 attatactga tagtgaattc aaacatgctt tagcaaggaa tcttcgttca ctgacaagag
      721 gaaaaaagtc cagtaaacaa cctatagcga ttttgcttgg aggtcaaagt ggtgccggta
      781 agactacaat tcatcgtatt aaacagaaag aatttcaagg aaatattgtt atcatagatg
      841 gtgatagttt tcgttctcag catccacact atttagaact gcagcaagaa tatggcaaag
      901 acagtgtaga atacaccaaa gattttgcag ggaaaatggt agagtcttta gtaaccgaat
      961 tgagtcattt gggatacaat cttttgatag agggaacttt acgaactatt gatgttccaa
     1021 agaaaacagc acaactatta aaaaataagg ggtatgaggt acaattagcc ttaattgcaa
     1081 ccaagcccga attgtcctat ctgagcaccc ttatccgata cgaagaactg tatgctatca
     1141 atccaaatca agcacgcgca actccaaaag aacatcatga tttcattgta aatcatctag
     1201 ttgataatac acgacaattg gaagaactgg ctatctttga aagcattcaa atttaccaac
     1261 gagatagaag ttgtgtatat gattcaaaag aaaatacaac ttcagcagca accgttcttc
     1321 atgatttact atttggagaa ttgaatcaag tggagaaaga gatgcttaaa tcaggagaag
     1381 aaagattgaa agatttaact aattgaaatg gttgttagaa cacaaatttt agaatatagt
     1441 ctacttataa cttttgttag ttgtttgagg tgggcaaaaa gtttttaaaa tcaaaaaaaa
     1501 cgcatagtat caggtgttga aaaaacttga tattatgcgt tttcttgttg gaagatttac
     1561 tccattttct tcaggaattg agattttgcc catctctttt tattatattt aaggatattt
     1621 gaaagctgta ttttagaaaa acaaatatgg agtttttaat acggacgtaa tatagtataa
     1681 atgctgattc aaagtgaagt tttatatgtt ttttgctttt gttgagtata aaatctatta
     1741 actaaagtcg aactacaaac attgatttaa gataaagtaa actgtaacct tcagaaatgt
     1801 ctcttactcg atttcagaat gaatcactga cataggatta aaagaactaa gtatttcttt
     1861 ttgttttgaa tgagatacgt gagtatagat ttgtgtgact gatatagaac tgtgtccaag
     1921 aatttgttga atgtatcgaa tatctacatc attatctaga agcattgttg caaagctatg
     1981 tctaaacata tgtggtgtaa tagttttaga taagctattt tgttcaacga ttctctttaa
     2041 aattaaacgt acactttgct ctgacaatgg tttaggcaaa tgtttccctg ggaataggaa
     2101 atcattagat tcctttccgg ttttatttat atatgtttct aatatattga aggtcgtttg
     2161 atctcctaaa
//
LOCUS       DAVLYV010000147         2133 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLYV010000147 DAVLYV010000000
VERSION     DAVLYV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.147"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..520
                     /locus_tag="VYO70_002107"
     CDS             <1..520
                     /locus_tag="VYO70_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714250.1"
                     /translation="IAYGGIEIPLVAHLFKEKNEISFLFQNSHYSTQEVEVKRFRDSR
                     RNDSKSILLMDDNILTGRAMKNAAQKLSYRKYSVQFFHVRRLGLNRLSQVIQENSMNE
                     LQRYLTGIYKGGIFPAPYSKIKFGTNIAKQYLDELQIFTLSGDEILRLLYKNGLFSEE
                     SEVKVVRGNLYE"
     gene            517..1152
                     /locus_tag="VYO70_002108"
     CDS             517..1152
                     /locus_tag="VYO70_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEV1714251.1"
                     /translation="MTFPISEWKRNEEFDFFNKMDVPYLSMSSRIEVTQAINFHKKHS
                     ISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTSEDKLNFSSFTMGD
                     NLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVSTPMNLSKIDTVPRI
                     TWGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVLLQRFVNKINEYFQKK"
     gene            1220..>2133
                     /locus_tag="VYO70_002109"
     CDS             1220..>2133
                     /locus_tag="VYO70_002109"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1714252.1"
                     /translation="MSIFIGGAWPYANGSLHIGHAAALLPGDILARYYRQKGEEVLYV
                     SGSDCNGTPISIRAKKENKSVKEIADFYHKEFKETFEKLGFTYDLYSRTDSPLHHEIV
                     QELFLQLYEKKFLYTKKIKQLYCTFDNQFLPDRFVEGKCPNCGTHSRGDQCDNCSAIL
                     DPIDLVDKRCSICSNEPEVRETEHFYYVFSEFQNLLETYLNDAEETVRWRKNAINLTK
                     RYLREGLPDRAVTRDLPNGIPVPIDGFRDKKIYVWFEAVAGYYTASVDWAQKLQNNIT
                     DFWNNRTKSYYVHGKDNIPFHTIIWPAIL"
BASE COUNT          770 a          285 c          400 g          678 t
ORIGIN      
        1 gatagcttat ggcgggatag aaattccctt ggttgcgcac ctatttaaag aaaaaaatga
       61 aatatcattt ttatttcaaa atagccacta cagtactcaa gaagttgaag taaaaaggtt
      121 cagggacagt cggagaaatg attcgaaaag tatcttatta atggatgata atattttgac
      181 aggaagggct atgaaaaatg ctgctcagaa attatcatac cgaaaatatt cagtccaatt
      241 ctttcatgtc aggaggcttg gcctaaatag attatcacaa gttattcaag aaaattcaat
      301 gaatgaattg cagaggtatt taacaggaat atataaagga gggatttttc ctgcaccgta
      361 ttcaaaaatt aaattcggta ctaatattgc taaacaatat ttagatgaat tacaaatttt
      421 tactctatct ggagatgaaa tcttaagact actttataag aatggcttgt tctcagaaga
      481 gagtgaagtg aaagtggtaa gggggaattt atacgaatga catttcctat atcagagtgg
      541 aaaaggaatg aagaatttga ctttttcaat aaaatggatg ttccttattt atctatgagt
      601 tcacgaatag aagtaacgca ggctatcaat tttcataaaa aacattcgat atcactttat
      661 gccataatat cgtggtgtgt tatgagtgca ataaattcta ttccagaatt actaatggat
      721 accgacggaa agattgtctg gcaatacaat caaagaggtt gttctttcac tacccttaca
      781 tcggaagata agcttaattt ttcttcattt acgatgggag ataatttaat tgaatttgtt
      841 tctgcgttca acataaataa acagaaggca gaagaagggc aaaaaccaaa tattgacaag
      901 aataacattg cttatctttc ttgtgttcca tggattgatt ttttacatgt aagcactcct
      961 atgaatcttt caaaaattga tacagttcct cgaataacgt ggggaaaagt tattcaagag
     1021 aaccaaagat atttttgtac agtgaattta caaattaatc atggaatggg agatggatta
     1081 cacgtatcta atttttttgt gttattgcaa aggtttgtaa ataaaataaa tgaatatttt
     1141 caaaaaaagt aatgattgaa aagatattgc atttaaataa tgttataata aaaataaaga
     1201 atttaaggag aaatacaata tgtcaatttt tattggagga gcatggccat atgcaaacgg
     1261 ttcgttacat attggtcacg cggcagcgct tttaccgggg gatattcttg caagatacta
     1321 tcgtcagaag ggagaggaag ttttatatgt ttctggaagt gattgtaatg gaacccctat
     1381 ttctatcaga gctaaaaaag aaaataagtc tgtgaaagaa attgctgatt tttatcataa
     1441 ggaatttaaa gaaacttttg aaaaattagg ttttacatat gacttgtact cgcgaacaga
     1501 ttctccacta catcatgaga tagtacaaga attatttttg caattatacg agaagaaatt
     1561 tttgtataca aaaaaaatta aacaactcta ttgtacattt gataatcaat tcttacctga
     1621 taggttcgtt gaaggaaaat gcccgaattg tggaacccat tcaagaggag atcaatgtga
     1681 taattgctca gcaattttag atcctataga tttagttgat aaaagatgtt ctatttgttc
     1741 caatgaacca gaagttcgtg agacagaaca cttttattat gtttttagtg agtttcaaaa
     1801 tttgttagag acttacttaa atgatgctga agaaacagtg agatggcgaa aaaatgcaat
     1861 aaatctaact aaaaggtatt tacgggaagg tttacctgat cgagctgtta ctcgtgatct
     1921 accaaatggg attccagttc ccattgatgg ttttagagat aaaaaaatat atgtctggtt
     1981 tgaagcggta gcaggttact acacagcaag tgttgattgg gcgcaaaaac ttcaaaataa
     2041 tattactgat ttttggaaca atcggacaaa gtcatattat gttcatggaa aagataacat
     2101 cccatttcat actattattt ggccggcaat act
//
LOCUS       DAVLYV010000148         2091 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLYV010000148 DAVLYV010000000
VERSION     DAVLYV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.148"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            66..230
                     /locus_tag="VYO70_002110"
     CDS             66..230
                     /locus_tag="VYO70_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1714253.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            309..1037
                     /gene="yaaA"
                     /locus_tag="VYO70_002111"
     CDS             309..1037
                     /gene="yaaA"
                     /locus_tag="VYO70_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1714254.1"
                     /translation="MKILIPTAKEMNTDLPSIEATPLKPESQAVLDALALYSASQLES
                     FYKVSAEKATEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(1315..1863)
                     /locus_tag="VYO70_002112"
     CDS             complement(1315..1863)
                     /locus_tag="VYO70_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1714255.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
BASE COUNT          638 a          379 c          453 g          621 t
ORIGIN      
        1 aaaaaggttt atttaataaa tatatgttat aataaggtaa attaagccta atggaggaca
       61 aagaaatggg tttttatttg atggttgctt caatgctact cggactgctc gctttgaaga
      121 taggtttttc tcagttcaag gagaaaaaag ataaattctt atctatctta acaagtttag
      181 ctggcatagc ccttgttctc gcggctgtct ggttaggatg gcccaaataa atagaaaaac
      241 ctcagtcaaa ccgaggtttt tcaaatgaat ttcttggttg tggctaaaaa atatgctaca
      301 ctatcaatat gaaaatttta atcccaacag caaaagaaat gaacacagac ttgccaagta
      361 tcgaggcaac tcctttaaaa ccagaaagtc aggccgtgct tgatgccttg gctctctatt
      421 ctgccagtca attggagagt ttctacaagg tatcagctga gaaagcgacg gaagaatttc
      481 aaaatatcca agctttgaaa aggcaaactg ctcaacacta tccagccttg aaactttttg
      541 atgggcttat gtaccgcaac attaagagag ataagctgac cgaggcggaa caagattatc
      601 ttgaaaatca tgtcttcatt acctcggctt tgtacggtgt tgttccagcc ttgtcaccca
      661 tggctcctca ccgtttggat tttttgatga aattaaaagt cgctggtaag actttgaaga
      721 gccattggaa ggcagtctat gatgaagctc tgaagaagga agaagtgatt ttctctctct
      781 tgtcatcaga gtttgagact gtattttcta aggaaatcag agcaaagatg gtgaccttca
      841 aattcatgga ggatagaggc ggtcagctga agattcactc aactatctcc aagaaagcgc
      901 gcggggcctt tctaacagct ttaatagaaa atcaagtaca aactgtgggg gaagcacgtc
      961 gcttgaactt tgctggattt gtttaccgag aagatttgtc acaaccacag ggattggttt
     1021 ttgtaaagga agtatagaaa tcagatattt tgtatttggc aatatgtatc atcagctgtt
     1081 ttagtttata aatgagaaaa aaagcgaaca agctagtctt ctgagtgtca gaaaagtagt
     1141 cttgttcgct tttattatgt tatactcttc gaaaatctct tcaagccacg tcagcgtcgc
     1201 cttaccgtat atatgtcact gacttcgtca gttctatcta caacctcaaa gcagtgtttt
     1261 gagcagcttg cggctagctt cctagtttgc tttttgattt ttattgagtg ttagttactt
     1321 gatagcttcg accaagtctt gagcttgttt ttcaagtgag tttaggactg tttcttcaag
     1381 aaccaatttt ccgtctgccc aggcagagtc attaacacgt gcagcagtga aatcaccaac
     1441 gccttgtgta cggataaatg gcaagaggtc tttgtagata gcgaaaagtt gatcgtgccc
     1501 tgcattggct acagatgata ctgtgacaaa cttgtcttga agggcagaaa cgccacgtgt
     1561 atcagacaag tcaagggcac gagatagcca gtcaagcaag tttttcactg taccagggat
     1621 agagaagttg tagactggag agaaaatcca gatagcatcc gcaacgagaa ctgcttcacg
     1681 agcagcagct acagctggat gtgttggaac ttccaaatct tggctgaaga gaggaagggc
     1741 tgaataatca aggtagctaa cttccgcttt accagcaagt gctttctcag cttcgagcgc
     1801 catttggtgg ttgaaagaac cttgacgtag tgatccgacg ataaataata cttttttaga
     1861 catgatgtgt ctcctttttc ttaaaatttc gagaactctc tcgttgttat aaaatatatt
     1921 acaacaaaac aaaagaactt gcaagaaatt tgctcagagt ttatttgaaa attgtaaaat
     1981 ttcggaaact ctagtcattt gtgactgaaa aagtaagaac tgtttcactt tcaaaatcgg
     2041 ttttgtttga agggagaaat agaattggtt gctcaaagca cagctttgag g
//
LOCUS       DAVLYV010000149         2058 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLYV010000149 DAVLYV010000000
VERSION     DAVLYV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.149"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>2058
                     /locus_tag="VYO70_002113"
     CDS             <1..>2058
                     /locus_tag="VYO70_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEV1714256.1"
                     /translation="VLSVTAMNNNEFITNLDEANKIIVHYADGTKDYFNLSSSSEGLS
                     NVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILESVELQSQTMYQHLNRLGDYRVNA
                     IKDLYLEESFTDVKENLTNLITKLVQNEEHQLNDSPAARQMIRDKVEKNKAALLLGLT
                     YLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIEIGSKENNIKGSRTFDAFGQV
                     LAKYTKSGNLDAFLNYNRQLFTNIDNMNDWFIDATEDHVYIAERASEVEEIKNSKHRA
                     FDNLKRSHLRNTILPLLNIDKAHLYLISNYNAIAFGSAERLGKKSLEDIKDIVNKAAD
                     GYRNYYDFWYRLASDNVKQRLLRDAVIPIWEGYNAPGGWVEKYGRYNTDKVYTPLREF
                     FGPMDKYYNYNGTGAYAAIYPNSDDIRTDVKYVHLEMVGEYGISVYTHETTHVNDRAI
                     YLGGFGHREGTDAEAYAQGMLQTPVTGSGFDEFGSLGINMVFKRKNDGNQWYITDPKT
                     LKTREDINRYMKGYNDTLTLLDEIEAESVISQQNKDLNSAWFKKIDREYRDNNKLNQW
                     DKIRNLSQEEKNELNIQSVNDLVDQQLMTNRNPGNGIYKPEAISYNDQSPYVGVRMMT
                     GIYGGNTSKGAPGAVSFKHNAFRLWGYYGYENGFLGYASNKYKQQSKTDGESVLSDEY
                     IIKK"
BASE COUNT          761 a          298 c          367 g          632 t
ORIGIN      
        1 gttctttctg ttaccgcgat gaacaacaat gagtttatca caaacctaga tgaagctaat
       61 aaaattattg ttcactatgc ggacggtaca aaagattact ttaacttgtc ttctagcagt
      121 gaaggtttaa gtaatgtaaa agaatatact ataactgact taggaattaa atatacacct
      181 aatatcgttc aaaaagataa cactactctt gttaatgata taaaatctat tttagaatca
      241 gtagagcttc agtctcaaac gatgtatcag catctaaatc gattaggtga ctatagagtt
      301 aatgcaatca aagatttata tttagaagaa agcttcacag atgttaaaga aaacttaaca
      361 aacctaatca caaaattagt tcaaaacgaa gaacatcaac taaatgattc tccagctgct
      421 cgtcaaatga ttcgtgataa agtcgagaaa aacaaagcag ctttattact aggtttaact
      481 tacctaaatc gttactatgg agttaaattt ggtgatgtta atattaaaga attaatgcta
      541 ttcaaaccag atttctatgg tgaaaaagtt agcgtattag acagattaat tgaaatcggt
      601 tctaaagaga acaacattaa aggttcacgt acattcgacg cattcggtca agtattggct
      661 aaatatacta aatcaggtaa tttagatgca tttttaaatt ataatagaca attgttcaca
      721 aatatagaca atatgaacga ttggtttatt gatgctacag aagaccatgt ctacatcgca
      781 gaacgcgctt ctgaggtcga agaaattaaa aattctaaac atcgtgcatt cgataattta
      841 aaacgaagtc accttagaaa tactatactc ccactactga atattgataa agcacatctt
      901 tatttaattt caaattataa tgcaattgcc tttggtagtg cagagcgatt aggtaaaaaa
      961 tcattagaag atattaaaga tatcgttaac aaagctgcag atggttatag aaactattat
     1021 gatttctggt atcgtctagc gtctgataac gttaaacaac gactactaag agatgctgtt
     1081 attcctattt gggaaggtta taacgctcct ggtggatggg ttgaaaaata tggccgctat
     1141 aataccgaca aagtatatac tcctcttaga gaattctttg gtcctatgga taagtattat
     1201 aattataatg gaacaggagc ttatgctgct atatatccta actctgatga tattagaact
     1261 gatgtaaaat atgttcattt agaaatggtt ggtgaatacg gtatttcagt ttacacacat
     1321 gaaacaacac acgtcaacga ccgtgcgatt tacttaggtg gctttggaca ccgtgaaggt
     1381 actgatgctg aagcatatgc tcagggtatg ctacaaactc ctgttactgg tagtggattt
     1441 gatgagtttg gttctttagg tattaatatg gtatttaaac gcaaaaatga tgggaatcag
     1501 tggtatatta cagatccaaa aactctaaaa acacgagaag atattaatag atatatgaag
     1561 ggttataatg acactttaac tcttcttgat gaaattgagg ctgaatctgt gatttctcaa
     1621 caaaataaag atttaaatag tgcatggttc aaaaaaatag atagagaata ccgtgataac
     1681 aataaattaa atcaatggga taaaattcga aatctaagtc aagaagagaa aaatgaatta
     1741 aatattcaat ctgttaatga tttagttgat caacaattaa tgactaatcg caatccaggt
     1801 aatggtatct ataaacccga agcaattagc tataacgatc aatcacctta tgtaggtgtt
     1861 agaatgatga ccggtatcta cggaggtaat actagtaaag gtgctcctgg agctgtttca
     1921 ttcaaacata atgcttttag attatggggt tactacggat acgaaaatgg gttcttaggt
     1981 tatgcttcaa ataaatataa acaacaatct aaaacagatg gtgagtctgt tctaagtgat
     2041 gaatatatta tcaagaaa
//
LOCUS       DAVLYV010000150         2013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLYV010000150 DAVLYV010000000
VERSION     DAVLYV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.150"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            979..2013
                     /locus_tag="VYO70_002114"
                     /pseudo
     CDS             979..2013
                     /locus_tag="VYO70_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
BASE COUNT          628 a          420 c          516 g          449 t
ORIGIN      
        1 aaagccgata tcagctcact caatgtgact gctgaaaata tccggcaatc tgtgaagagc
       61 cttgagacag acacgcagaa caagctaaat cagaagttga gtcaggctga atttgaggtg
      121 cgagccggct ctatacgtca ggaaatcctg aatgcaacca aggataaagc cagcaagtca
      181 gaactcacgc agacagctga ggagctagct agtaagatag cgagtgtgca cttagggcgc
      241 agaaatttgc tgaaaggcac aaaagagctt gcgagataca agccggttag tgaatataat
      301 ggttttaaag ttatcagaac agtcgcagga gcaactagat atcaggatag ctatgtggaa
      361 agaaccgtta taccaacggc tgggacagag tatatagcta tcttttatgc acgagccagt
      421 gaaaatgact atcctgtgcg ctgtcatttt tacaatccta acacggttgt atcatcagaa
      481 aacagcagcg gatataagtc aaggtcgtca gatggcttgt ctattatccg tctctcgaca
      541 gactggcagt tgtgctgggt taaatggacc caaaccgcaa cagatcaagc caagacggtc
      601 atcattggcc gccatggccc tcaagtaggc ggtaaagagg gggtatgggt tgaaatctgc
      661 gcccctgcca tttttgaggg aaatcttgca ggtgactggt caccagcata cgaagaccaa
      721 gacgaacgtg tctcagcggt cgaatccaac tttaaacagc gtgctgattc actcgaagct
      781 ggtgtaagcc gtctgactga aggccttaga accaaagccg atatcagctc actcaacgta
      841 actgctgaaa atatccggca atctgtgaag agtcttgaga cagacacgca gaacaagcta
      901 aatcagaagt tgagtcaggc tgaatttgag gtgcgagccg gctctatccg tcaggaaatc
      961 ctgaacgcaa ccaaggataa agccagcaag tcagaactca cgcagacagc tgaggagctc
     1021 tctagtaaga tagcgagtgt gcaggtcggg ggtaggaatt atatccgggg tacaaagcgc
     1081 atgatgctag ccagaggatt gtgggcatca ggtactttta gaccgtcagg cgctgggacg
     1141 gcaaagacga ttgatgtatc agacagtcca gcaactggct ttgataaagc gatacgattg
     1201 acctcaagca atgctagaga ccaaataggt attgctcaag atggatttta tatctcgcaa
     1261 ggcacataca cgatgtcttg ttgggtcaaa ggcagaagag gtcaaaaggt caagctacaa
     1321 acttattggc aagtccatga taattcgggt atttcaccca tctttacatt aaaggatgaa
     1381 aattggacaa agctatcgtt tactagcgct agaaataggg ctggagtcgc atcaattggc
     1441 tatgtgtatc tcgtaaatgc tgaggtcgga gaatatttag atgttcttgc gccccagctg
     1501 gaagacggaa gtttggcaac aagctcaaaa gaagctcctg aagatataga aggtcagatt
     1561 tcaacagttg aatcgacctt caaacagcgt gctgattcac tcgccgctgg tgtgaatcgt
     1621 ctgactgaag gccttagaac taaagccgat atcagcgcac tcaacgtgac tgctgaaaat
     1681 attaggcagt cggtgaagag tcttgagaca gacacgcaga acaaactaaa tcagaagttg
     1741 agtcaggctg aatttgaggt gcgagctggc tctatacgtc aggaaatcct gaacgcaacc
     1801 aaggataaag ccagcaagtc agaactcacg cagacagctg aggagctggc tagtcggatt
     1861 gcgagtgtgc aggcatccgg tcgaaatcta ttcttgaact cactattcaa gcaggatatt
     1921 ccaaaaacag gaatttggac aacgagtaca tatacggcta ctatcgatag cgaaagtaag
     1981 tatcttggac acaaggctct taaaattata ggt
//
LOCUS       DAVLYV010000151         2013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLYV010000151 DAVLYV010000000
VERSION     DAVLYV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.151"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            74..805
                     /locus_tag="VYO70_002115"
     CDS             74..805
                     /locus_tag="VYO70_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714257.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDENSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            820..1971
                     /locus_tag="VYO70_002116"
     CDS             820..1971
                     /locus_tag="VYO70_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1714258.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
BASE COUNT          735 a          256 c          381 g          641 t
ORIGIN      
        1 aagctaatta ttgtagtaat ttctttaaga aaatgatata attcattaaa aagtatttgg
       61 aggaggaaga attatgttga atcaggatct ctttgattcg cttgaggcac aaaaaattgt
      121 agatactttg atgaaagggc aaaaagatta tgtagatgaa cgtctagaaa aaagagagac
      181 aatgatagtg tcgaatggtt atgcatggac gcgacctaat catattgata ctgcatttgc
      241 atcagcagat ttgtttgagt ataaattaca attagcagga cagacttggg gatatttaga
      301 atttgaaaca aatacagaaa aatatgggaa agtattgtta attataaagg gtaagaagcg
      361 acttacgaac caatttcctt tggtacaaaa aaataagagt ggctacttat ttgaatatgc
      421 tcagatgaat acactttatc ttaatcaaca ttcttcctac aaaaatgatg aaaatagtca
      481 ttcctttcca attcagatgg agttagtttc tgatgaaatg attcaagaaa ttgaacaagc
      541 tactaaaaat tcgaatatcg aaaaatttat gattttaact tatgaggcgg actcagaaaa
      601 caatattata tctgtagatg ttgttatgcc tgatgcacga actggtcagt tacacttgat
      661 tcaagatttg tctgagtata ttcaatcaag ttcgtaccat ttcgaagaag ctaaatatca
      721 agatattcct aatttttcag aattatcgga aacagaagat tttgaaatta ttccaagaat
      781 agaaaaacaa gaaggtcaaa agtagtaagg agtatgtaaa tgtttaatgg tcgggtattg
      841 aaagaattac ggctgttaaa tggtttaagt agagcagaat tagctcagag aattaattta
      901 acggaacaag ccatttggca gtttgagtcc aacgaaacga aacctaaatt atcaaccaaa
      961 atgcatttgg ccaaccaatt tcatgttgat ttaacttatt ttgaacagga agaagagagc
     1021 attcgatttg attcttctgt aattgccttt agaaatgcag acctagcaac acggaaaaca
     1081 atagatattc aaactatgta tttacataag gtagatagtt tgattgatta ttttgaaagt
     1141 tttgtaatta tacctaatat tataattcat gacctaagta atgtagtgag tgaatcttat
     1201 cataagggag aatccattga ggaattggct ctttatgcca gggaaaaatt aggtatttca
     1261 aaagataatc atgatttgct ttataaatta gaacgttcag gcatctatat cgtggaacga
     1321 ttaattaatg gccaagctga tgcttatagc gcatggtcaa aattgggaag accttatatt
     1381 gtgttgggaa cgaataaatc atctgtacgt cgaaattttg acttagctca tgagctagga
     1441 catattcttt tacataaata taaagatatg aatgaagatg gcgatcgttt ggagcaagaa
     1501 gcaaattatt ttgcatcatg ttttttattg ccaaaagaag agtttttagt caaatttgaa
     1561 gagagggttg gcaagcgtgt cagcaatcct gatagttata ttttattgaa gtcggatttg
     1621 aatgtttcga tacaggcttt agagtatcga gcttttaagt taggattatt gactccaaag
     1681 caacattctt acttttatcg tcaaattgcg caaaaaggtt acaaaatgat tgaacctttg
     1741 gatgatcaaa tttttgttaa aaaaccaagc aaagtaaaga gtattctgga cgtcgttttg
     1801 agtaatcatc tagtcagtct agcgactata atgtctaaac aaagtattcg tttacagttt
     1861 ataagcgaaa tattttcagt cgaaatgaaa ttttttgatc agtatcaaga agatagaaga
     1921 acagatcgat ttgataacat catccctttg tacaaaagaa ataatttata atttaactgt
     1981 gaaataattt catgaaggac atatggcaga aat
//
LOCUS       DAVLYV010000152         2010 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLYV010000152 DAVLYV010000000
VERSION     DAVLYV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.152"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..228
                     /locus_tag="VYO70_002117"
     CDS             <1..228
                     /locus_tag="VYO70_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1714259.1"
                     /translation="HVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLALQNEFADF
                     VAQVDKSQLAIQKSLEELETLKKSLMQEYFG"
     gene            288..524
                     /locus_tag="VYO70_002118"
     CDS             288..524
                     /locus_tag="VYO70_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1714260.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            521..934
                     /locus_tag="VYO70_002119"
     CDS             521..934
                     /locus_tag="VYO70_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1714261.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            1052..>2010
                     /locus_tag="VYO70_002120"
     CDS             1052..>2010
                     /locus_tag="VYO70_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1714262.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKF"
BASE COUNT          729 a          311 c          388 g          582 t
ORIGIN      
        1 catgttttaa ggaataataa ttatagtcga gtgatatcag gaagtgctca gcctcagtta
       61 ccaattacaa aattaaaaaa aatacttctc cccctccccc cactagccct ccaaaatgag
      121 ttcgcagact ttgtagccca ggtcgacaaa tcacaattgg caatccaaaa atctctggaa
      181 gaacttgaaa ctttgaagaa atctctgatg caggagtatt ttggctgata ttctgccatt
      241 gtaattacgg taatgatttg ttataatact tcaaaggagg aaatcagatg gtagtaaaaa
      301 caagaaaaca aggaaattca atcaccatta cgattccaag tgaatttaat attccaagtg
      361 gtgttaaata cgaagcgaaa ttgttaccaa gtggtgagat tatctttact cctgaagaat
      421 tggggcagca ggtttcttat gtatctgatg atgcctttga cttaaattta gataaaatat
      481 ttgacgaata cgacgatgtt ttcaaagctt tggtggaaaa atgacaatct atctgacaga
      541 aaagcaaatt gaaaaaataa atgctttagc aattcaacgg tattctccaa atgagaaaat
      601 tcaaacagtt agttcttctg ccttaaatat gattgtgaac ttaccagaac aatttgtctt
      661 tgggaagtct ctttatccaa caatttttga taaagcaacg atactatttg tccaattgat
      721 aaagaagcat gtttttgcta atgctaataa aagaactgct ttcttcgttt tggtcaaatt
      781 tttacaatta aacggctatc gtttttctgt aacggtagaa gaagcagtaa aaatgtgtgt
      841 aaccatcgca gtagaagctt taactgatga aaaaatgaca agctactcca aatgggtttc
      901 tgaacattct gttagagaaa aggtcaaaaa gtaacctagt atgctggatt tgaatgagca
      961 caagaaaata aatgaacaga caatattaga attctgtaat gcagaaactg atattgtctc
     1021 tttttattga tgaataagaa agtgagaaat tatggaatca aaagttacaa ttatcatgca
     1081 agaaatgtta cctcttttaa ataatgaaca attactagcg ttgagagaga gtttagaaca
     1141 tcatctagta gacggaaaaa agcagcagaa gtattcgaat aataacctgt tgcaactatt
     1201 tattaccgcc aagcaggtag agggctgtag ctcaaaaaca attcgttatt accagaggac
     1261 gattgaaaac ttgtttaatg ctattaaaga gtctgtgaca caactcacaa cagatgattt
     1321 aaggagttat ttagcaaatt accagtctga aaaggattgt agtaaggcaa atttagacaa
     1381 tattaggcgt atattgtctt ctttttttgc ttggcttgag caagaggaat atatcattaa
     1441 aaatcccatt cgacggataa agaaaattaa gactgagcaa aatgtgaagg aaacttatac
     1501 tgatgaacat ttggaaatta tgcgtgataa ctgtgaaaat ttgagagatt tggcaataat
     1561 agacctacta gcatcgacag gtatgcgtgt aggggagctt gtacagttga atcgttcaga
     1621 tattgatttt gaaaacagag agtgtgttgt ctttggtaaa ggaaagaagg agagaccagt
     1681 atattttgac gctcggacga aaattcattt aagaaattat cttaacgaca gaaaagatag
     1741 tcaccctgct ctttttgtaa cgttagttgg aaaagtccag aggcttggaa ttgctggtgt
     1801 agagattcgc ttaagaaagt taggagacaa actcggtata caaaaggttc acccacataa
     1861 gttcagaaga actttagcga ctaaggcaat tgataaaggt atgcctatcg aacaagtcca
     1921 aaaactgcta ggtcatagca agattgacac aaccctggcc tatgccatgg tcaatcaaaa
     1981 taatgtcaag cattcacacc aaaaattcat
//
LOCUS       DAVLYV010000153         1984 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLYV010000153 DAVLYV010000000
VERSION     DAVLYV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.153"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..694)
                     /locus_tag="VYO70_002121"
     CDS             complement(<1..694)
                     /locus_tag="VYO70_002121"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1714263.1"
                     /translation="MKYQLIVSTMNQQDNSLIEKMNIKSDAIIINQSNSFSYHETKLK
                     NSIVKWYEFNERGIGLSRNTGFMRSDADIIQFADDDMIFTDTYYEDVLLEYQKHPEAD
                     VILFSNKCLNEDRMPYQVNNFRRINRFEGVKFGGARITVRREKILHNNITFSLLFGGG
                     AKYGAGEDVTFIQDCIKAGLRVYKSPIIVSTMKQDSSTWFKGYDRKYYKDKGALLAAN
                     FPSISEVGVYIQA"
     gene            complement(710..1633)
                     /locus_tag="VYO70_002122"
     CDS             complement(710..1633)
                     /locus_tag="VYO70_002122"
                     /inference="COORDINATES: protein motif:HMM:NF012745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1714264.1"
                     /translation="MIKRLAILMATYNGEKYIKEQIESILNQDTDFDFTLIIRDDGST
                     DNTKNIIREYVDSGKIIFIEGQNKGAARGFISLLCDNPGYDYYAFSDQDDVWNHNKLQ
                     KGIWSLSKYTGPALYCSNCELVDSELNSIGRNTHRKRPNYTLESILCLASCAQGCTSV
                     FNKELASIIQENDVPDTFIMHDSLITCLCALIGGKIIYDEIPSMKYRMHDDNIFGMVS
                     AKQNVLNVIKDRISEITKKRTISMYDQAECLLKVYKKYIDSNNQDICRIVIDSKVSLK
                     ARLKLIFNKNLQHTTLNKTITKKLEILFGND"
BASE COUNT          666 a          373 c          228 g          717 t
ORIGIN      
        1 aagcttgtat atatactcct acctcactta tagaagggaa atttgctgct aataaagcac
       61 ctttatcttt ataatatttt ctatcatatc ctttaaacca tgtagaagaa tcttgtttca
      121 ttgtagaaac aataataggt gatttatata cccgtaatcc tgccttgata cagtcctgaa
      181 taaatgttac gtcctctcct gcaccgtatt ttgctccccc cccaaataaa agagaaaacg
      241 ttatgttatt atgcaatatc ttttctctcc tgactgtaat tctagcacca ccaaacttaa
      301 caccttcaaa cctatttata cgtctaaaat tattaacctg ataaggcatt cgatcttcat
      361 taagacattt attcgaaaaa agaattacat cagcttctgg atgtttttga tattctaata
      421 atacgtcttc ataatatgta tctgtaaaaa tcatatcgtc atcagcaaat tgaataatat
      481 cagcatccga tctcatgaaa cctgtgtttc gacttaaacc aatacctctt tcattgaatt
      541 cataccattt tacaatcgaa tttttcaatt tcgtctcatg gtaagaaaat gagtttgatt
      601 gattaatgat aattgcatca gattttatat tcattttctc tatcagactg ttatcctgtt
      661 gattcattgt tgatacaatg agttgatact tcatatctaa actcctttat tagtcattgc
      721 caaataatat ctctaatttt tttgttatgg ttttatttaa tgtagtatgc tgcaaatttt
      781 tattaaaaat cagcttaagt cttgccttta gcgatacttt agaatcaata acaattctac
      841 atatatcttg attgttagaa tctatatatt ttttataaac ctttagtaaa cactctgctt
      901 gatcgtacat actaatcgtt cgcttttttg ttatttcact aatacgatct tttataacat
      961 ttagtacatt ttgcttagct gacaccattc caaatatatt atcatcatgc attctatatt
     1021 tcattgatgg aatttcgtcg taaattatct ttccacctat cagtgcacat aaacaagtaa
     1081 tcaaagaatc atgcataata aaagtatcag gcacatcatt ctcctgaatt atcgaagcta
     1141 gctctttatt aaatacagat gtacatcctt gtgcacaact agcaagacaa agaatagatt
     1201 caagagtata gttcggtctc ttcctatgtg tattacgacc tatactattt aactctgaat
     1261 caactaattc acaattagaa caataaagtg ctggcccagt atatttggaa agactccata
     1321 tacctttctg taatttatta tgattccaaa catcatcttg atccgagaaa gcatagtaat
     1381 catatccagg attatcacat aacaaagaaa taaatcctct tgccgcccct ttattctggc
     1441 cttctataaa aataattttc ccactatcaa catattctct aattatattt ttagtattat
     1501 ctgttgaacc gtcatctcta ataattagag taaaatcaaa atctgtatct tggttgagaa
     1561 tactttcaat ttgctctttt atatattttt cgccattata tgtggccata agtattgcta
     1621 atctcttaat catttcccct catcctttaa aataactatc tgaaatacat tctaattaga
     1681 actgttttca taactcaagg tataacttga ttcaaaaagt aacatagcta atgcaatata
     1741 tgggaatcta ggtgcatcag tgattatcaa attaatacaa gaaaacatca aaaaaactat
     1801 aactagcttc attaataagt cataatgagc tggaattcta atcgtattat ctttaattat
     1861 cttcaagctt gtataaaacc aacttattaa ccaaattagc ccgataatcc cacttctaaa
     1921 cataagtcca atcaaaccta catcgcttgg ataataacgt cctaatggac cggtaacaat
     1981 agaa
//
LOCUS       DAVLYV010000154         1903 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLYV010000154 DAVLYV010000000
VERSION     DAVLYV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.154"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(322..1215)
                     /locus_tag="VYO70_002123"
     CDS             complement(322..1215)
                     /locus_tag="VYO70_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1714265.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
BASE COUNT          644 a          380 c          209 g          670 t
ORIGIN      
        1 tacctccttt ttagtttaca cttcctacat cataaataaa catactaaac cctcacaatc
       61 tccaaataac tcttctatat cattgaaaat tgagtttcat cctctatact aatgtaggaa
      121 acctattaaa acattccttt aatccaatat aagaatacct tgttccatta atctctaccc
      181 gatattatag atagaaccaa attcctatac ttaaaaggac aaataacgtt ccaaccataa
      241 taaacctcct ttctttttga gaacgttttc tttataggtt tagtgtagca aaaacatgaa
      301 aaaaacaaca tgacttagaa gtcaggttgt tgggaaatct atctgttttt ctgctaaaat
      361 tctctgaact aatacctgat cgtctagcaa ttctgctaga gaaatcgcct tttcatagaa
      421 actttctgcc tctaaaatat tcttgtctac aaataaagca tattttgcct ttagcaaaaa
      481 tagatgaggt cggtaaaaaa catgtcttgt tttagcaata attttctcaa atgtttgaat
      541 atatatcaga ctatcttctg tctgaatttg gataaatagg ctcagaagaa ctttttccat
      601 ttgaactagg ctagccttat cttcatattc tgaacatagc aatacttttt gagctaactc
      661 ctcaaaatgt tctttatctt ctaatcccat agcacaacaa aagaaataca attcaataat
      721 caacaaatca tttacagaaa aatatttctt ttttaataac tgctggaaat attcttccac
      781 taaccctaaa ccgtaggtca catctgaact accataaata tcaaaacgag cttgtgtcac
      841 atccacaatt aactgctctt cctcaggcaa attctcataa tagttttcat atatttcctc
      901 aagtatagct tcttttcgtt cgattcttcc cttatcagcg taggtttgag aatgaatcag
      961 ctcatctttg agttttagga atccttttgg aatctctata accttaacat ccacaatatt
     1021 ctgtaatgat actccaagtt tatccgcaat caataataat ttaggaagtg ttgctaaagc
     1081 ttgcccattt tcaatacgtg ctagctgacg aatcgtcaac tctgattcat caccacaaat
     1141 atctatctga gtttttccac aagatcgcct aatagttttg attttataac caatttcacg
     1201 tttcaactca tccatagtaa tactccttaa gttgattaca aagatttctt tttcttccta
     1261 attcttttgt caatcatata taaaacgata agcgaaaaca gcggtagaac aatataactc
     1321 ttttcactca ttatcagctg aaatcccagg gttgctagta tcttaaagat gaaaatattc
     1381 atcaaaaata gaaaaacaaa tactaaaaaa tattttaaaa ccttcttcat ttcttttctt
     1441 caactttcct aatttctaca cctattttat cgaaaatata tataaaactc caaataaaca
     1501 cttcgactgc actttttttg gcaaaaaata gacagaacaa acatcctacc tactaaaaat
     1561 atgatatgaa aggaaaatga ttttttactg acaccctagg caagcttggg acaaataaat
     1621 cattgatagc aacatttacg acatcctatt taaagttcct tcaatttcta atgtccccgc
     1681 tatttctaag ccacctctgg tatgatctat tcctagtcag aatagataac accgttctaa
     1741 attacgattc atagttactc cttttttgac tcgttactat aagactccta tttacatgcg
     1801 caaaataatt ttgattcaga ttcatcattt atagttttga ttatttttta acagattttt
     1861 ctgttttctt ctccgaagac tttaaaaatt tttttctaat taa
//
LOCUS       DAVLYV010000155         1872 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLYV010000155 DAVLYV010000000
VERSION     DAVLYV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.155"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(150..1781)
                     /locus_tag="VYO70_002124"
     CDS             complement(150..1781)
                     /locus_tag="VYO70_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1714266.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGDLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFETTL
                     NLAPEMTIAFAHGTFNITNTIIQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
BASE COUNT          569 a          367 c          391 g          545 t
ORIGIN      
        1 atttctaaca atgttttaga aatagaggtg tcctattcta gtttcaatat actatatatc
       61 tgcagaatac tctttcagtt tcattcagcc tagtcttgct taggaaaaag taaaaagcca
      121 tccaattatg atggatagct tcttggttct tagatttgtt ctgcaaaaac cttttcagca
      181 aggttcatag cgtggtctga tacacgagtg tagtgtgaga tgatgtcgat aaagttgacc
      241 ccagcttgtg ttgaacattc gcctttgttg aggcgtttga tgtgggtttt tctgagaaca
      301 cgttctatct tattgattgc ttcatgacgt tctacaagac tgcgtgcttt ttcaatatca
      361 ttgttttcca cactatccag agcatctttg ataaagtcac tagtttggcg gtaaacttcc
      421 tctaattctt tcaaggcggc atcagaaaat tcaacatttt tccgttgaag atagtcagtc
      481 agattgagta gagcctctgt gtggtctcca atccgttcca aatcacggga ggaatcaagg
      541 atattggtaa gcacttcact ttctttttgg ctgagagctt cgcttgaaag ggcaatgaga
      601 taacgtgtta attgctcatc gatggtgtta attgcttctt cggttttatg ccctttttca
      661 gcaacttttt catccaagtc aatgatgtac ttataggaaa ggtcaaaggc tttagcagcg
      721 tagtttccta agtgcaagag ctctttttta gcatttccta gagcgataga tggggcttgt
      781 ttgatgaaat gttcatcaag atataagggt tcgtatttga caacctcgtc ctctccagga
      841 ataatcttgg ttacaaagta agccagagct ccgataaatg gaaattggat aatggtgttg
      901 gtaatattaa aggttccgtg agcaaaggcg atggtcattt ccggtgctag atttagcgta
      961 gtttcaaacc aatgaatcag gacagtaaaa ggaactagaa aaataacgca gacgactgtt
     1021 ccgataacgt tgaaggcaac atgagctcct gctacccgtt tagctgcaat attagcccct
     1081 aaagaggcaa tgatggctgt aatggttgtc ccgatattgt caccaaatag aactggcaaa
     1141 gctccctgta ggtcaattag atcgccggcg tagaggtttt gtaaaatccc aatggtagcc
     1201 gaagaagctt gaattagcaa ggtcaagcca gtaccgacaa agacacccaa aacaggattc
     1261 ttacttagct caatcatata gtccttaaag acctgtaaat ccttgagtgg agccattgcg
     1321 ccgctcatga gattgagggc aaaaaagata ccaccgacac caaagaggat gcgtccgata
     1381 ttattgactg tccgattttt cgtaaaaaag agacagacgg caccgataaa gagcataggt
     1441 agggcatagt tacctaattt aaaaccgatg agaaaggatg tgacagttgt cccaatatta
     1501 gcacccatga caatcccgat agcctgacgt aaggttaaga gaccggcact gaccaggccg
     1561 actgtaataa ctgttacacc agaactagac tgaattagag cagtcatccc aataccaacc
     1621 agaactccaa agaaaggatt actagtatat ttgtcaatgt aaaaacgaag gcgatctcca
     1681 gcagcttgtt gcaaaccgtc tcccatggtc ttgatactat ataagaatag ccccagacca
     1741 cctaaaaagt gaaataaaat ttcctgccaa ttaatggaca tttctttttc ctccgaaaaa
     1801 taatcgcgga atatctccta ttctatttta aaggataaaa gtaaatctaa caagtgttaa
     1861 tgtcaagttt gt
//
LOCUS       DAVLYV010000156         1773 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLYV010000156 DAVLYV010000000
VERSION     DAVLYV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.156"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            55..757
                     /locus_tag="VYO70_002125"
                     /pseudo
     CDS             55..757
                     /locus_tag="VYO70_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(904..1749)
                     /locus_tag="VYO70_002126"
     CDS             complement(904..1749)
                     /locus_tag="VYO70_002126"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1714267.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT          600 a          361 c          356 g          456 t
ORIGIN      
        1 atagagctga cgtggtttga agagattttc gaagagtata aaaaggtgct aggcatgttg
       61 atttttcctt tgttaaatga tttgtcaaga aaaatcatcc atattgacat ggatgccttt
      121 tttgctgcag tggaaatcag ggataatcct aaactcagag gaaaacctgt cattattgga
      181 agcgaccctc ggcaaacagg tggacgggga gtcgtttcta cctgtagtta tgaggcaaga
      241 gcttttggtg tccattctgc catgagttcc aaggaagctt atgaacgttg tccccaggct
      301 gtctttatct cagggaatta tgagaaatac aagtctgtgg gactccagat tcgagctatt
      361 tttaagcgct atacagattg gtagactagg ctatgatctg tatcgaaagg ctcgtggcat
      421 tcacaactct ccagtcaaat ccaatcgcat ccgtaaatca atcggcaagg agaaaaccta
      481 cgggaagatt ctccgtgctg aggaagatat caaaaaagag ctgactcttc tatcagaaaa
      541 agtcgctctc aatctacatc aacaagaaaa agctggaaaa attgtcattt tgaaaatccg
      601 ctacgaggac ttttcaactc ttaccaaacg aaaaagtctt gctcaaaaaa cacaagatgc
      661 tagtcagata agccaaatag ccctgcaact ctatgaagaa ttaagcgaga aagaaagagg
      721 tgtccgccta ttggggatta ccctgactgg attttaaagc tcaatgaaaa tcaaagagca
      781 aactaggaag ctagccgcag gctgctcaaa acactgtttt gaggttgcag ataaagctga
      841 cgcggtttga agagattttc taagagtata aaaccttgaa gagttgcccc ttcaaggttt
      901 ttcttataca aataaacgga caaaactgta aagtagcaaa acactaccaa gcacgatacg
      961 gtatttacca aaaagggtga agtcgtgttt tttcacatag ctggtcaaga agcgaatagc
     1021 caccatgctg accgcaaaag ctactcccat cgcgaccaag agcaaaaaca attgcccaaa
     1081 gctcaagagt tctccggctt tcacaaattt gaaaatcttt aaggcactag ctccaaacat
     1141 aacaggaatc ccaagataga aggtaaattc tgtcacaact gaacgactgg ttccatttaa
     1201 caaaccaccg acaatcgttg cacctgaacg gctagtccct ggtaaaagag caagaacttg
     1261 gaagagtccg atatagaaag cggtcgtata aggaagcttg tccaactctg ttacacttgg
     1321 ctcgatagca cgcgctttat tgcgcttttc caaatagatg aaggcaaccc cgtagataat
     1381 caacatgaga gcaactgaaa ccatgttatg gaagtgggta tcaaaccaat catcaaattt
     1441 aaagacacca agtaaaggca aagtggcaat caagaccttc aaccatagtc tccaagtctt
     1501 acgaacttcc tgtttgtcct tagtcggttt aaaaggattg agcttgttaa aataaatcac
     1561 cataactgct aaaatagcac caagctgaat tacgacatta aacatggaca taaaggcttc
     1621 attttgattt tggtattgga taaactcctc tgctaaaatc aagtgacctg tactggaaat
     1681 cggcaaccat tccgtaattc cttcaacaat cccgaagaag atagatttta aaatttcaat
     1741 aagatacata gatttctcct tatattatat tta
//
LOCUS       DAVLYV010000157         1709 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLYV010000157 DAVLYV010000000
VERSION     DAVLYV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.157"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..589
                     /locus_tag="VYO70_002127"
     CDS             <1..589
                     /locus_tag="VYO70_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714268.1"
                     /translation="TLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIF
                     QAYNLIPSLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQK
                     VAIARAVIADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKT
                     DRALILKDGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            592..>1709
                     /locus_tag="VYO70_002128"
     CDS             592..>1709
                     /locus_tag="VYO70_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1714269.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYCKSY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLT"
BASE COUNT          549 a          226 c          314 g          620 t
ORIGIN      
        1 aactttatta aaatgtattt ctagtttgct tgaaccaaca agtggggaag taattttaaa
       61 tggaatcaac ccctataaaa tcagaaatgc aaaattgtca agtataagac gtaacgaagt
      121 atcttttata tttcaagcat acaatttaat accttccctg ccggtaatag aaaatatagc
      181 acttcctttg cgattatcac aaaaaaaatt aactattaaa aatgtagaaa acttactcaa
      241 aagaatgaag tttaatgctg gcttaaacga ttttgttgga actctgtctg gtggagaaca
      301 acagaaggtt gctatagcta gagcggttat tgctgatagt gatataatat ttgctgatga
      361 gccaactggg gctttagaca gcgtttctcg tgaagtaatt tttgaattat tgagagagtt
      421 agtaggggcg ggtaagtgtg taattatggt aacgcacgat atagaattgg cctcgaaaac
      481 tgatcgtgca ttaatattga aagatggaaa aattttcaaa gaacttcata gacctagcgg
      541 ggaagagttg tataaaatct tagaggtaca atcaactacg gaggaataga gatgctcaga
      601 ttagtttact atcaattttt acataataag aaacaatggt taggcgtttc accagtaatt
      661 tttgtttcga gtttagttat gggattggct gttaatgggg taattaatgt tgagaataat
      721 tcgcaagtat ttgtcggact tccagatcct aaaccaattt ttatgtttcc gatagtattc
      781 ggaggagtaa cattattctt tgtgctctct aacataataa atatgttggt tgaaattttt
      841 agagatgatt acgaacttct ggaggtgttg ggagcaagtc gccttcaact atcatttctt
      901 gtggggggac agatttttat catttcctcg attattagtt ttattgcata tctatgctct
      961 atatttgtta cgtcaaatta ttattatttt ttacaatact ttttcgggga aaatatatta
     1021 cctgatatcc aatttcaaac gagtgctgtt ggatgtatca ttacagtagt gttgatttca
     1081 tttttagcct ttctaagtgg ttgcttttat acgttcaaga agattagaaa taggaagagt
     1141 tctaaaataa ggcatgttct ttcaatagta aaaagaatac tgctattggc tggttttagt
     1201 gttatctggt tgctttcttt gcaacaaatt ttccaagata gcacaatttt agcgaaggcc
     1261 cagataatat tcaatattgt aatcttagat attgtgatta tttatcaatt atctcctttt
     1321 atacagtcat gttttatcaa actactttct ataattatat ttagaaataa ttttatgttc
     1381 attgtttcga agtggaattt actatattgt aagtcttata taaaatctat cagtgctgca
     1441 attacaggag ctatattact aatttctagt tttcaaatga tttctcagaa tatactatct
     1501 caatttcaag atgacagtga tttagagcta aaagtagcat ttattgtcta tgtaggagca
     1561 ccaattttga tagtacttgc aaatattatt tcaattgcat ttttgtcatc gcatcaagaa
     1621 aggattgaga ttcagcaatt tgaaatttta ggaacttcta attaccagat ggttaagatt
     1681 aaagttggtg aggctatatt tttgacatt
//
LOCUS       DAVLYV010000158         1693 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLYV010000158 DAVLYV010000000
VERSION     DAVLYV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.158"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..792
                     /locus_tag="VYO70_002129"
     CDS             <1..792
                     /locus_tag="VYO70_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1714270.1"
                     /translation="YNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEK
                     IRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQR
                     GKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTG
                     LGTLGRLAIYHENKNPYGWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKA
                     SGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHINALI"
     gene            849..1646
                     /gene="psrA"
                     /locus_tag="VYO70_002130"
     CDS             849..1646
                     /gene="psrA"
                     /locus_tag="VYO70_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1714271.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          611 a          239 c          302 g          541 t
ORIGIN      
        1 tataatagac tagaacagct agataaagaa tttccagata aactaaaaaa atctattctt
       61 caatatgcta tgcaaggaaa attagttgaa caagacccaa atgatgaatc agtcgaagtt
      121 ttacttgaaa aaatacgagc agaaaaacaa aaactctttg aagaaggcaa gattaaaaag
      181 aaagatttgg acatttctat tgtttcccaa ggagatgata actcttatta tgaggaagta
      241 ccttgtgaaa taccagaaag ttgggaatgg gtgaggttaa acgatataac ctcctacatt
      301 caaagaggga agtctccaaa atattcaaat attcctatat accctgtgat tgctcagaaa
      361 tgtaatcaat ggtctggctt ttctattgac ttagcaagat ttattgatcc cgaaacagtt
      421 catagttacc agaaagaaag attactaaga gatggtgatt taatgtggaa ttcaacaggt
      481 ctaggtacgt taggacgatt agctatttac catgaaaata aaaatcctta tggctgggct
      541 gttgcagata gccatgttac agttataaga gtcttatcgg gagttattaa ttgccatttc
      601 atctataatt ttctttcctc tcctattgtt cagtcagtta ttgaagaaaa agcgtcgggt
      661 agtacaaaac aaaaagaatt attaacgaaa actataaaag aatatttaat ccccctccca
      721 ccccttcctg aacaatccag aatcgttgat aaaatcgaac aatttttcgc ccatattaac
      781 gcactaattt agtataatag ttttacgtgc ggtggaattt ctatcaaaca taagatagga
      841 gttttacgtt gaaagatttt gaattacatt tgaagaaagc tggtttagct gaaaatactg
      901 tgcgttcgta cctttatggt gttcgatttt tcttggagaa ttacgaactc aaaatggaag
      961 atttatttga atacaaagga tatttattag acaattttaa acctaaaact gtcaatttgc
     1021 gactacaagg agtaaataaa tatctggctt ttattggtca tgatgatttg aaattaaaat
     1081 ttgtcaaagt acagcaaaaa ccgtttttag aagatgtcat tagccatgct gattaccttt
     1141 ttcttaaacg tagtttgaaa aaagatggta ttttaaaatg gcattttgtc gtttggtttt
     1201 taggtgcaac tggggctcgt gtaagtgaac ttattaaact aaaagtagag catgtagaaa
     1261 tcggttattt tgacatttac tcaaaaggtg gtaaaattcg tagactgtac attcctaaaa
     1321 aattaagaaa tagttgtctt agttggttag aatcagaaaa tcgccgaagt ggttatttat
     1381 ttttgaataa atttaacgaa ccaattacag caagaggagt tgctcaacag ttaaaaaatt
     1441 atgctgataa atacaaaatg aatcctaaag taatttaccc tcattctttt aggcatttat
     1501 ttgctaagaa ttttttagcg aagtataatg atattgcctt gcttgcagat ttgatgggac
     1561 acgaaagtat agaaactact cgaatttatc taaggaaaac agctactgaa caacaaaata
     1621 ttgtagataa aattgttaat tggtaaaaaa taacaggtgg tcaaactgac tacctgctat
     1681 ttttgtgatt atg
//
LOCUS       DAVLYV010000159         1686 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLYV010000159 DAVLYV010000000
VERSION     DAVLYV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.159"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(367..1347)
                     /locus_tag="VYO70_002131"
     CDS             complement(367..1347)
                     /locus_tag="VYO70_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1714272.1"
                     /translation="MKNSALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
BASE COUNT          503 a          364 c          312 g          507 t
ORIGIN      
        1 aaggcgaagc tgacgtggtt tgaagagatt tttgaaaagt attaacacct acagacaagc
       61 ctgacgatag caagaactac cctactcgat aggtatcggc ttttgctttc tgaaaaaaat
      121 tattttaagc aatttgacaa atctagcaac aaaaaattct ataaatacaa tagattgaaa
      181 ctagaatagt acacctttgc ttctaaaaca ttgttagaaa tcgatttgac tgtcctgatc
      241 gatttgtcct gttcttgttt catttcacta tatttccatg ataaaacgca tagtatcaag
      301 ttttcttaat cccctgatac tatgcgtgtt tgtaattttt aagattttgt gcttagagtc
      361 gactccttat tttagatatt taaaaggaat ctcacttcca cagagccagt tgtagacttg
      421 gtcattaaca aatacattca tggcttcgtg agcatactca ggcatgatac gataggtttt
      481 atcgcaggtc agacgattat aaatcgcaaa ctgggtaatg ggatagcaaa catcgtcgtc
      541 caagcccgta atcatcttaa cctcaccttg gatacgatgg gcaagatttt tgacatcgat
      601 ataggcaagg gtcgccatga tttcctcctc tgtttcatgg aaggggtcgt gaaacttgaa
      661 ataacggaaa agttcgtcgt aagcctcgct agtattacca atctcaatca cccgtctgaa
      721 gtctgacaag aagggataaa tggcaactgt tttctgaatt cgaggattga gcgctgctgc
      781 aactagagct agagcccctc cttgtgaggc accatagcta gaaagacgct tctcatcaac
      841 ctgagacaga ctagcaacaa tttcgaccaa ctggtaaata tccagataaa catccttata
      901 aaagaggtgg tcccgacctt ccatagcacc acggataata tgccccttca cggtatttcc
      961 taaaggagaa cgcaagccgt cttgtgagta acctgactgg ccccgcacat ccatggaaac
     1021 aacaccgtaa ccagctacgg tgaagcccag catgtcggcc cagtcccagc cacgtcccat
     1081 ataaccatga aaatggaaga ttaatggaac cttctcctca ctctttggaa gaacaatgcg
     1141 tgcatagacc tttccttcct tgcttccttc aaatgttaac tcatagcact tgacttgagg
     1201 aatgtggaaa tctctttcct ccaagtggta ggatggaagc gtggaaacat ttttcacttc
     1261 cccatcccag aaagcatcaa agtcttccgg aacctcatcc cttcctctat aggtcttaat
     1321 ttcttctagc aaagctgaat ttttcatagc atgctccttt tattttgtaa tcgttaccac
     1381 aactgtagca tatctatgta atcaatgcaa gaaagaaggg taaatagaaa gtgaatttag
     1441 attctttact tttaggatga tgggctgaaa gtagttttag aaacgaaaaa agagcatttc
     1501 gctctctctt ctgtttatac tagttttttg cttccttctt ttggaaagtg atttggtaat
     1561 ttactttaat cgttactgtc atatgagagt cctcgtttct ttttgatgac tttagtataa
     1621 gagccaaacc taaaaactag cttaatactc ttcgaaaatc tcttcaaacc acgtcagcgt
     1681 cgcctt
//
LOCUS       DAVLYV010000160         1662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLYV010000160 DAVLYV010000000
VERSION     DAVLYV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.160"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..312
                     /locus_tag="VYO70_002132"
     CDS             <1..312
                     /locus_tag="VYO70_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714273.1"
                     /translation="KYTMGFNYFVSLNYLLYKVGFLGIIYDNEALRDLSKQYLNKNIS
                     ELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCVPSQYDRFFYKDRPYIFSRKSPIK
                     G"
     gene            627..1604
                     /locus_tag="VYO70_002133"
     CDS             627..1604
                     /locus_tag="VYO70_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714274.1"
                     /translation="MKNKRYFFDTILIILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLILFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYIGIKHVSIRR"
BASE COUNT          565 a          215 c          276 g          606 t
ORIGIN      
        1 aaatatacta tgggattcaa ttactttgta agtttaaatt atttactata taaggttgga
       61 tttttgggaa tcatttacga taatgaagca ttaagagatt tgtcaaaaca atatttaaac
      121 aaaaatattt cggaattaca agatggttct ttcgagggcg gtatacaaga tgctgttgcc
      181 tctaaacaaa tagtagttat aaatgaattt atagcttgtt taaattccag atgcgtgcca
      241 tcccaatatg atagattttt ttataaagat agaccttata ttttttccag aaagagtcct
      301 attaaggggt gaaaatttgt tacatttgga ttaacaagtg tcctagggat tagaaataac
      361 tctagtatag ctagatattt ttgagtaact agaaatatca ttaggctacc catatgattg
      421 ccagaaaaga tggctgatga atcttattta tactgttgca gtaggcattg tctaggataa
      481 ataagctatc ttgatggggg agattggaat aattttagta gaaacattaa aggattatgg
      541 acaagtagcg taggattagg tgatgcaatg tgctaagttg gcttggtgtt agcagtagta
      601 ttatttaaca atggaaagta gactaaatga aaaataagcg ttattttttt gacacaattt
      661 taataatttt actgcttata agcacgatat tttgtgtcag cccagtattt ataaaattgg
      721 acatcttagg aactccctct catgccatct taacctttgt attggcgata ccgttattct
      781 atattttgtc tcaatgctta catactttgc tattactagt ttcctcaatc ttttgtaaat
      841 taagaccaat ttatttttat tttatatttg tgatcattat aggagctagg aaatactatc
      901 gaattctctt tcaccaacta atgggattta gtccaggaat agcagtgttt tataaggaga
      961 gtcaaacaac taagaatctg tttaaatttt actacttctt gtactttaca acactaattt
     1021 catactattt tttctttact tttgtgtatg acaaaccgtt gcttttgcct cttattttat
     1081 tttctattat catagcatta gtacaaaaac tttatagaat tgagaatcaa caattatttc
     1141 tgttaaaatc aaaggtacta acaatactgg aatctaagaa gaattgtgag tttaatcttc
     1201 aggattatca tgaaatttgg aaattgcaat caaaatctga gttgccatgc gtggctttat
     1261 cttatatcag tttaatcaag ccatatcttt cagagagtgt gcgtgaacaa attgatttat
     1321 tagaagttaa gcgatttaaa aaaataaatc atccaatatc actttatggt atgttggatg
     1381 ttataaaatt aaatctatat ttacggcatt ataatgaaaa aaataaatat gaatcaatgt
     1441 taaagaaaat tttagaggta agaccagact ttgttttaat cgagcagaat attgatgata
     1501 gtttaaatag ctcgcaacca ttatctttat ctttagccat ttcagaaatc caacttttgc
     1561 ttgaagtgta tatagggatc aaacatgttt caattaggag ataaaactag aataatattt
     1621 gccattatag gggcaagttg catattagtc agtatgtatc tt
//
LOCUS       DAVLYV010000161         1662 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLYV010000161 DAVLYV010000000
VERSION     DAVLYV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.161"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            6..1007
                     /locus_tag="VYO70_002134"
     CDS             6..1007
                     /locus_tag="VYO70_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1714275.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1219..1448
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          556 a          223 c          333 g          550 t
ORIGIN      
        1 ctgaaatgaa aaatataaat gggaaaaagg taactattta tgatattgca cgtttatcag
       61 gtttttctcc aaaaacagtt tcacgagtta ttaatggtgg agtaaatgtt aaagaagaaa
      121 cttatcaagc gattcaaaaa gttatagagg aattgtcgta tattccaaat gcgtatgcaa
      181 aaaatttaac taaaaaagaa gctataaata tattgatttc tgtaaaaaaa atcgactctt
      241 ttcctttaat ttggtttcat acgctgttag ataaagtgtt acggacgtgt aaagaatttg
      301 gtgttaatgc tatagttgag tactttggag aggaggatac aattagtaat tcgattatat
      361 caagcacagg tagcctagta gatggtgtta ttgtttttta tgaaagtgta gatgatatca
      421 gaattcagta tttaaagaag aaccatatgc cttttcttgt tttcggtgaa tctcaaacgt
      481 ctggagtagt ctatgtatct aataataatt ttcaagctac ttatgatatg atgaaagctg
      541 taactgaaga aaaatttaaa aatatgtggt tgcttatggg aggagaatct catgttaata
      601 aggatcgtga aagaggtgtg cgttcttttc tgaatgataa aaattacttt atggatttaa
      661 aagtcattta tggattatct acaattgatt cagtttattc ttatgcaatg cagcacttga
      721 ctacccaaaa ttatccggac attatttttg tttcaggaga tgaaaaggtt cagggcttga
      781 ttcgtgcgtg ctatgagaaa ggaattctta ttccagatga tatttctatt attggatttg
      841 ataatattcc tatttctcag tattatactc cagctttgtc tacaattgct cccaattatg
      901 ttaaattggc taaagaaatg atagaaggtg ttttagcaat tattaaaggg gaaagtgtca
      961 catctgttga agtttctcct aaatttgtta ggagacagag tttctagtat tgtttaattc
     1021 ttgaaaatcc tagtggaaaa gatggagtgt tctaaaataa gttctgttta gcctccattc
     1081 agtttctatt gagttggatc caatttctaa caagtgggac atgttcatgt tcgatctaag
     1141 gaagacatgg tcacattcga atgatatttt caaaaaaatc gtaaaaaagt cttgacaaag
     1201 aaaaaacaaa gtattaaaat aagttcaaca aatcagaaaa gtaactattt ttgatcttca
     1261 gggagcctgt ggtggttgtg aataggtggt tggaagtagt gaaagtgggc tgattttgaa
     1321 aatgaatttg aaataatgaa aattcggtgc gcacacctta cagtgcagct tgttgttaaa
     1381 caaggcagag atgtaaaggg atagtccctt tataattgag gtggcaccgc gttaccaacg
     1441 ccctcacacg gaagtatatt ctgtgtgtgg gcttttttct atccgtcgtt tggtttatct
     1501 tttattaggg ccttaagtcg ctttgatgaa cttgagttct atctacagct ccttgcctac
     1561 tactaaaagc aaacaaaaag gctggactaa tacgaaaaaa gctagtaata ctcaatgaaa
     1621 atcaaagagc aaactaggaa actagccgca ggctgctcaa aa
//
LOCUS       DAVLYV010000162         1576 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLYV010000162 DAVLYV010000000
VERSION     DAVLYV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.162"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..200
                     /locus_tag="VYO70_002135"
                     /pseudo
     CDS             1..200
                     /locus_tag="VYO70_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818294.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
     gene            248..886
                     /locus_tag="VYO70_002136"
                     /pseudo
     CDS             248..886
                     /locus_tag="VYO70_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838036.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
BASE COUNT          548 a          199 c          272 g          557 t
ORIGIN      
        1 atcttccttt gatagtgata aaatatttga gattgtactt gagttggttc ggactgaaaa
       61 gctatctaat ttatatttta tttcacttac taatgaaaaa gaacattctt tatttgaata
      121 tttgactcaa aaaataaaaa actcaactca tatacaagtg caagaacgtt tacaattgtt
      181 gtctctatat cctactctaa aaaactttgc aaagtatgat tgtatatttg ttgatgatga
      241 agatttgatg cacacgctat tagacttatt tgattggttt actataattg atagaccaaa
      301 aatatgtttg ataggttcac gtgtttggtt cgaaaaaaga aatctttttt atatacattt
      361 agattataga atattggcac tcaatactgc cgaaaattta gaaagtggaa atagttcaat
      421 tgtaccagtt aaagatggca aacaaatttt aactaatcaa ttatttgatg gagaatgtga
      481 tgatatttat ttacttactg tccattcacc aatgaataaa gccttagaaa ttttatcaga
      541 aaagtataaa caaatttctg ggaaaaatat taaaattatc gagaaaagat atgatgaact
      601 tttggagatg attgagtctg gcgatgtttc atccagtttt gatatgatta gaatggatat
      661 ggcttggctt ccaacatttg ggaaaaagta ttttcaagag attacaagct ttagacaaac
      721 aaaaagtatt aatcaaaata tttctaagtc ggtgtctcga gagtatatgt atattgatca
      781 gaagcaatat acttttccat tggatgttag tagtcagatt ttagtatata gaaaagactt
      841 atttgaagat tcatttttac aaagacgata ctttgaaaaa acaaaaagaa aattaactgt
      901 accaaaaagt tatcaagagt ttgatgagtt aagtgaattt tttacattaa cagaaaatcc
      961 attttcacct acactttatg gccatagttt agctttaagt tcttcagtga gagcagcatg
     1021 tgaatttatt cctcggtttc gtgagagatt agcacaatct agaatgaatc ttgctgtact
     1081 tcctcaggtt ttaactgagt atgagaaaag ttatcagttt accgaaaaat catttaattc
     1141 ttcgtggaac gatttcgtta cgaacttaaa cagtggtaaa acttcaatgg aaataatttt
     1201 ttcaaattat actagtccat tatttgatgg tctcaatgtt tctgctcagt ttgagtttgc
     1261 aactgccact atacctggta atactcctgt gataggtggt ggctcaattg gaatttctaa
     1321 atattcaaat agagtggaag aatgtctaaa ctttattaat tggttatatt ctgaagagat
     1381 ttctatttta ctaacatctc tgggtggttt tttaccatca aagtatgtca tgcaaaatag
     1441 gatgttacaa tttcaatacc catggttatc tagtttagaa gatacattta gtattgggag
     1501 tagaacaaaa tggtacggct ttaatactga ttttagattt gagcagatgt tagggcaaga
     1561 gttaatagaa agtgtg
//
LOCUS       DAVLYV010000163         1557 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLYV010000163 DAVLYV010000000
VERSION     DAVLYV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.163"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>1557
                     /locus_tag="VYO70_002137"
     CDS             <1..>1557
                     /locus_tag="VYO70_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1714276.1"
                     /translation="TEAKATFERTAQGLRTDLSAIQEYVNKDGQRQEALQRYTREEST
                     RQATAVRELVNRDFVGKATYQEDVKGINQRIEAVKTSANKDIASQIASYRQSVDGKFT
                     DISSQITTYKQDVGGQISGLSNRLTSSEQGTTTQISNISNRINSNKQGTDNQISNLKT
                     QVATNKDNAERQMGRISDQVSANKANADSQFANVTNQLARKVETTDFQRVKETSKLYE
                     RILGNTENGIADKVARMALTNQLFQVEVGKYSVSGPNLIKNSDFKNATNEWGSTQNLG
                     RLVKHSFYHNGQKDLMRLSNATKNENFLYSHRFNLERNTDYVLNFRGFNNSALASYDV
                     YILGRRAGESDGFTIVKKVVSSKKLSTSRCEDVSVTFNSGEMDNAYIRFDNNGSSSGT
                     ADLYITEVDLYKGYKPRTWQPHPEDAVADANKKLEATQTKMTQLAGSWAVQNINSAGD
                     IISGINLGANGHNRFVGKLTHITGETLIDRAVIKSAMVDKLKTGNFEAGSVTTTILDA
                     EAVTAEKLKVD"
BASE COUNT          525 a          286 c          354 g          392 t
ORIGIN      
        1 actgaagcta aggctacctt tgagcggaca gctcagggct tgcgaaccga cttatcagct
       61 attcaggaat atgtaaataa agacggtcag cgacaggaag ccctacagcg ctatactcgt
      121 gaggagagca cgagacaagc gacagcagtc cgtgagctgg tcaatcgtga tttcgttggt
      181 aaggctactt atcaagaaga tgttaagggt atcaatcaaa ggattgaagc tgttaaaact
      241 agtgcgaata aagacatcgc tagtcaaatc gctagctatc gtcaatctgt agatggtaag
      301 ttcacggata tttcaagtca gataactact tataagcaag atgtgggcgg tcaaatcagt
      361 ggtctatcaa atagacttac aagcagtgag caaggaacca ctactcagat ttcaaatatt
      421 tcaaatcgga taaacagtaa taaacaaggc acagataatc agatttcaaa tttaaagact
      481 caggtcgcta caaacaagga taatgctgaa cgacaaatgg gtagaatatc tgatcaggtt
      541 tctgcaaaca aagcgaatgc tgatagtcaa tttgcgaatg tgaccaatca actagcgcga
      601 aaagtagaga ctactgactt ccagcgtgtt aaggaaacca gtaaacttta cgagcggatt
      661 ttgggcaata ctgaaaatgg aattgcggat aaggttgctc gcatggctct gactaatcaa
      721 ctgtttcagg ttgaggttgg gaaatatagt gtaagcggcc ctaacctcat taagaatagt
      781 gattttaaaa atgctacgaa tgaatggggc tcaactcaaa atttaggaag attggttaag
      841 catagctttt atcacaacgg gcagaaagac cttatgcgtt taagtaatgc aactaaaaac
      901 gaaaactttt tgtatagtca ccgttttaat cttgaacgaa atactgacta tgtactgaat
      961 tttagaggat ttaacaacag tgctctcgca agctatgatg tttatatttt gggacgaaga
     1021 gcaggcgaga gcgatggatt cacaatcgtt aagaaagttg ttagcagcaa gaaactatct
     1081 acctctagat gcgaagatgt ctcagtaact tttaattccg gagaaatgga taatgcttac
     1141 attcgttttg ataacaatgg ctcatcatca ggaacagctg atttgtatat tacagaagtt
     1201 gacttgtaca aaggttataa acctagaaca tggcaaccac atccagaaga tgcagtcgca
     1261 gatgcgaata agaagcttga agccacgcaa acaaaaatga ctcaactagc tggctcatgg
     1321 gcagttcaaa acatcaactc ggctggagat atcatctctg gaatcaatct tggcgccaat
     1381 ggacataacc gctttgttgg gaaattgacc cacatcactg gagagaccct gattgacaga
     1441 gcagtcatca agtctgccat ggttgataag ctcaaaacgg gcaattttga agctggttcg
     1501 gtcacgacta cgatattaga cgctgaagcg gtaactgctg agaagttgaa agttgac
//
LOCUS       DAVLYV010000164         1557 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLYV010000164 DAVLYV010000000
VERSION     DAVLYV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1557
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.164"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>1557
                     /locus_tag="VYO70_002138"
     CDS             <1..>1557
                     /locus_tag="VYO70_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1714277.1"
                     /translation="TEAKATFERTAQGLRTDLSAIQEYVNKDGQRQEALQRYTREESA
                     RQATAVRELVNRDFVGKATYQEDVKGINQRIEAVKTSANKDIASQIASYRQSVDGKFT
                     DISSQITTYKQDVGGQISGLSNRLTSSEQGTTTQISNISNRINSNKQGTDNQISNLKT
                     QVATNKDNAERQMGRISDQVSANKANADRQFANVTNQLVRKVETTDFQRVKETSKLYE
                     RILGNTENGIADKVARMALTNQLFQVEVGKYSVSGPNLIKNSDFKNATNEWGSTQNLG
                     RLVKHSFYHNGQKDLMRLSNATKNENFLYSHRFNLERNTDYVLNFRGFNNSALASYDV
                     YILGRRAGESDGFTIVKKVVSSKKLSTSRCEDVSVTFNSGEMDNAYIRFDNNGSSSGT
                     ADLYITEVDLYKGYKPRTWQPHPEDAVADANKKLEATQTKMTQLAGSWVVENINSAGD
                     IISGINLGANGHNRFVGKLTHITGETLIDRAVIKSAMVDKLKTGNFEAGSVTTTILDA
                     EAVTAEKLKVD"
BASE COUNT          524 a          280 c          358 g          395 t
ORIGIN      
        1 actgaagcta aggctacctt tgagcggaca gctcagggct tgcgaaccga tttatcagct
       61 attcaggaat atgtaaataa agacggtcag cgacaggaag ccctacagcg ctatactcgt
      121 gaggagagcg cgagacaagc gacagcagtc cgtgagctgg tcaatcgtga tttcgttggt
      181 aaggctactt atcaagaaga tgttaagggt atcaatcaaa ggattgaagc tgttaaaact
      241 agtgcgaata aagacatcgc tagtcaaatc gctagctatc gtcaatctgt agatggtaag
      301 ttcacggata tttcaagtca gataactact tataagcaag atgtgggcgg tcaaatcagt
      361 ggtctatcaa atagacttac aagcagtgag caaggaacca ctactcagat ttcaaatatt
      421 tcaaatcgga taaacagtaa taagcaaggc acagataatc agatttcaaa tttaaagact
      481 caggtcgcta caaacaagga taatgctgaa cgacaaatgg gtagaatatc tgatcaggtt
      541 tctgcaaaca aagcgaatgc tgatcgtcaa tttgcgaatg tgaccaatca actagtgcga
      601 aaagtagaga ctactgactt ccagcgtgtt aaggaaacca gtaaacttta cgagcggatt
      661 ttgggcaata ctgaaaatgg aattgcggat aaggttgctc gcatggctct gaccaatcaa
      721 ctgtttcagg ttgaggttgg gaaatatagt gtaagcggcc ctaacctcat taagaatagt
      781 gattttaaaa atgctacgaa tgaatggggc tcaactcaaa atttaggaag attggttaag
      841 catagctttt atcacaacgg gcagaaagac cttatgcgtt taagtaatgc aactaaaaac
      901 gaaaactttt tgtatagtca ccgttttaat cttgaacgaa atactgacta tgtactgaat
      961 tttagaggat ttaacaacag tgctctagca agctatgatg tttatatttt gggacgaaga
     1021 gcaggcgaga gcgatggatt cacaatcgtt aagaaagttg ttagcagcaa gaaactatct
     1081 acctctagat gcgaagatgt ctcagtaact tttaattccg gagaaatgga taatgcttac
     1141 attcgttttg ataacaatgg ttcatcatca ggaacagctg atttgtatat tacagaagtt
     1201 gacttgtaca aaggttataa acctagaaca tggcaaccac atccagaaga tgcagtcgca
     1261 gatgcgaata agaagcttga agcaacgcaa acaaaaatga ctcaactagc tggctcatgg
     1321 gtagttgaaa acatcaactc ggctggagat atcatctctg gaatcaatct tggcgccaat
     1381 ggacataacc gctttgttgg gaaattgacc cacatcactg gagagaccct gattgacaga
     1441 gcagtcatca agtctgccat ggttgataag ctgaaaacgg gcaattttga agctggttcg
     1501 gtcacgacta cgatattaga cgctgaagcg gtaactgctg agaagttgaa agttgac
//
LOCUS       DAVLYV010000165         1510 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLYV010000165 DAVLYV010000000
VERSION     DAVLYV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.165"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..670)
                     /locus_tag="VYO70_002139"
     CDS             complement(<1..670)
                     /locus_tag="VYO70_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714278.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIDSKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFMFSYILLFVQ
                     SYLGSNTAWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILIAI
                     FENLL"
BASE COUNT          570 a          267 c          187 g          486 t
ORIGIN      
        1 caagcaaatt ttcaaaaata gctatcaata tgattggtgt aaggtttttg tattcacatg
       61 tcaagtattc tgagtatcgt gcagatgata tttcttttac ataacttttt gaaagcaaga
      121 ttttttcaaa ttcatcatct attattaatc ttctatttcc aatccaagcg gtattgctac
      181 ccaaataaga ttgtacaaat agcaatatgt aagagaacat gaacgtaaat actgaaagag
      241 gtagaattct atgcgtaaaa aaatagacga taatggtagc caggagtaaa gatatctctc
      301 gtgacaatcg aattcttttc agcatctttt ttaacagtcc gtaaggttgt ccttggcaat
      361 attcttgaac taaattaatc ggaatgacat ctctcaccat ttctggaaag tagagtaagt
      421 ttataggctg aaatctccag ctcccatcgt aagtaaatgg atatagaacc aaatacttaa
      481 aagatttttt tcttataaac ttaattatca caatatgaat gactgttgca atgaagtata
      541 ttaaatataa aagtaaaacc gccatcaata tacttacaaa aatccccttg gaatcaatca
      601 atggaaaata aaatattgca actagaagcg aataaagtcc cgctaataaa tatttctgat
      661 catctttcat tcaaaacacc atattattta ctaagaatac atcaatacaa tagagcaact
      721 ctgtaagtca atatttgata aagcaaagct ttaaaccctt ccccagacgt tagcttttat
      781 tcaacccact acagttgaca aggagcccaa attattgata aaaactttag tcatcatcgt
      841 ctctataaaa atattttcta tacactcttg ataataccaa aattaatgcc aatatcatac
      901 caagagctat aataatagaa tttattagat ttattttagt atatccaatc actagtccaa
      961 taattggact agcagacatg cttagatata ataaaaataa caaaaaatga atttcaccaa
     1021 ttcctgcaaa aaaatgtttc atagctatag ttttacgccc ataaataccc atcaaattta
     1081 ccgtctacga tatctaaacc atatgcaatg gctcctccta atgagaagac ttcagcaaca
     1141 gtagccgcaa tattaagagc attagtaata gttgccatac taactccacc aatatatctt
     1201 gttatagcac ttagttacct agataagata tcctttagct tgcttccaaa atttaagtct
     1261 acacctataa tatgagttca tattagtact gctaaatcct atctatagta atgtcaaaca
     1321 cctaaatcaa gtcatctctc caattcatcc aagaaatgaa gaaaggcaaa aaatctttaa
     1381 aataagaaaa gtgcatatta tcaggtgttc aaaaatcttg ataatatgcg ttatataata
     1441 gaaaaattta ctcaatttca ttttcaaatt gagttttttc ccagcctcat ttttaattta
     1501 tatcttcaat
//
LOCUS       DAVLYV010000166         1504 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLYV010000166 DAVLYV010000000
VERSION     DAVLYV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.166"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..614
                     /locus_tag="VYO70_002140"
     CDS             <1..614
                     /locus_tag="VYO70_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1714279.1"
                     /translation="GLTSSLILFLLAKKDGFSAARLIILGIGFQAVTRAGTSFLLLKV
                     ARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFNKRLEIIELGEEIAIGL
                     GANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALNIGKKRSPILAGLVGIL
                     LVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRNI"
     gene            614..1408
                     /locus_tag="VYO70_002141"
     CDS             614..1408
                     /locus_tag="VYO70_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714280.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHINS"
BASE COUNT          534 a          239 c          250 g          481 t
ORIGIN      
        1 gtggactaac atcatcttta atattatttt tactagctaa aaaagatggt ttttcagcag
       61 caagactgat aatattaggt attggttttc aagctgtcac aagagcaggc acctcatttt
      121 tattgttgaa agtagcaaga tatgaattac aagaagttat gagatggctc agtggctctt
      181 tatcttttac aaagttagat gacatacctc ttgttctaat agtaagtatt attgctacta
      241 tattagtttt attttttaat aaaagactag aaattattga acttggtgaa gaaatagcaa
      301 tcggacttgg agcaaatccc gagctttcaa ggcttgtttt aattttttgc gctgtatctt
      361 taactgcttt ttctacttca attacaggac caatagcttg tatatctttt ttagctggtc
      421 ccatagcctt aaatattggc aagaaaagaa gcccaatatt agctggattg gttggaattt
      481 tactagtttt gttatcagac atattctctc aaaatatttt accagctaga tatccagtag
      541 gtgttgtaac tggcttgtta ggttcaccat acttaatata cttactaata aaaatgaaca
      601 ggagaaatat ataatgaaag gtctgtggtc aaataatctt acctgcggtt atgatgaaaa
      661 aataatcttg gaaaatataa atataaaaat acctgaagaa aaaatatcag ttattattgg
      721 gtcaaatggt tgtgggaaat caacactcat taaaaccttg tctcgactta taaagccatt
      781 agagggagaa gtattgcttg ataataaatc aattaattct tataaagaaa aagatttagc
      841 aaaacacata gctatattac ctcaatctcc aataatccct gaatcaataa cagtagctga
      901 tcttgtaagc cgtggtcgtt tcccctacag aaagcctttt aagagtcttg gaaaagatga
      961 ccttgaaata ataaacagat caatggttaa ggccaatgtt gaagatctag caaataacct
     1021 agttgaagaa ctttctgggg gtcaaaggca aagagtatgg atagctctag ccctagccca
     1081 agatacaagt atcctacttt tagatgagcc aactacttac ttggatatct catatcaaat
     1141 agaactatta gacctcttga ctgatctaaa ccaaaaatat aagacaacca tttgcatgat
     1201 tttgcacgat ataaatctaa cagcaagata cgctgattac ctatttgcaa ttaaagaagg
     1261 taaacttgtt gcagagggaa agcctgaaga tatactaaat gataaactag ttaaagatat
     1321 ctttaatctt gaagcaaaaa ttatacgtga ccctatttcc aattcgcctc taatgattcc
     1381 tattggcaag caccatatta actcttaata attgaatatt ggaattcatt taaaagtcgc
     1441 aaattttgaa ctgaccctca aaagttaggc caaaaaacta actaaaggag gtcagttttt
     1501 taat
//
LOCUS       DAVLYV010000167         1457 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLYV010000167 DAVLYV010000000
VERSION     DAVLYV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.167"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..111)
                     /locus_tag="VYO70_002142"
     CDS             complement(<1..111)
                     /locus_tag="VYO70_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEV1714281.1"
                     /translation="MSLLETAKRHQLNSEKYLSYLLECLPNEETLVNKEVL"
     gene            complement(183..632)
                     /locus_tag="VYO70_002143"
     CDS             complement(183..632)
                     /locus_tag="VYO70_002143"
                     /inference="COORDINATES: protein motif:HMM:NF015038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1714282.1"
                     /translation="MVNCEQLEAYRQLEEAALVGCWAHVRRKFFEVPPKQADKSSLGA
                     KGLAYCDQLFSLERDWEALPADERLQKRQEHLQPLLEDFFAWCRRQSVLSGSKLGRAI
                     EYSLKYEETFKTILKDGHLVLSNNLAERAIKSLVMGRSKRVQWTLLA"
     gene            complement(705..1295)
                     /locus_tag="VYO70_002144"
                     /pseudo
     CDS             complement(705..1295)
                     /locus_tag="VYO70_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1219..1413)
                     /locus_tag="VYO70_002145"
     CDS             complement(1219..1413)
                     /locus_tag="VYO70_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1714283.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEEDGDVPS"
BASE COUNT          394 a          324 c          278 g          461 t
ORIGIN      
        1 taaaacctct ttgtttacga gagtttcctc gtttggaaga cattctagaa gataggatag
       61 atatttctca ctatttagtt gatgacgttt agctgtttcc aataagctca taataatagc
      121 tcttgcttta gctccttcaa aaactttgag aaaacaacca ccctcgcttt tttaaactga
      181 gcttaggcta aaagagtcca ctggactctt ttactccgtc ccataaccaa tgatttaatg
      241 gcgcgttcag ctagattatt ggaaaggacc agatgtccgt cttttaaaat ggtcttaaag
      301 gtttcttcat acttgaggct gtattcaatt gcccttccta gttttgaacc cgataaaact
      361 gactgacgac ggcaccaagc aaagaagtct tccagtaggg gttggagatg ttcttgacgt
      421 ttctgtagtc gttcatcagc tggcaaagcc tcccagtctc tttccaagga aaataactga
      481 tcacaataag ctaaaccttt agctcctaag gatgatttat ctgcttgctt ggggggcact
      541 tcaaaaaact tccttctcac atgcgcccaa catccaacaa gtgcagcttc ttccagttga
      601 cgataagctt ctagctgttc gcagtttacc aagcttagcg tcgccttact cctaaacttt
      661 ggactgctat cgcataggca gaactagagg actaaagtcc taagttactg ccgcaacatg
      721 tcacaatgaa catagccaga ataatctcct aggaattctt gtactactaa accactccga
      781 cgctgatcat ggtggtacag cgtgattgct tgattctcag ctttcccaga caaaaaagtc
      841 caatagtaag tcaactgact atcactctct agaacccgat aagaggtttc atccgcatga
      901 agtaagggct tgagtcaata gtctcgttat aaaggggctt taggaatagg agctttcacg
      961 attttatcac tcggattttt atcactgcat gcttggcatt tgtaagcgtg ttggatatga
     1021 tctatgcgtt ttaattgcgc aggaataaag accaattctt gtccttgaag actccctcca
     1081 atctctttta gatctccctg acaatcaggg caaatcctct cttctacttg atgatgaact
     1141 tcttctgaat caaattgggc aagaagagct tggcgtttcc ctttagattt cttacgtttg
     1201 taggtaattt cctctgtttc aactgggaac atctccatct tcctcagatg gagactcttc
     1261 ctcaaaaagg ttgagttgtc cagaggggca aacacttttc tcagaggatt ttccatagag
     1321 cttttgagtc agataagcca cttgttcacg aagaagggca agttcattgg tgagactatc
     1381 aattgtagca ctctgttgtt gaatgatttt caatcttctt ccatgagctt ccctctctgt
     1441 tttctaactt tattata
//
LOCUS       DAVLYV010000168         1409 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLYV010000168 DAVLYV010000000
VERSION     DAVLYV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.168"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            83..1261
                     /locus_tag="VYO70_002146"
     CDS             83..1261
                     /locus_tag="VYO70_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1714284.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDNYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          517 a          258 c          202 g          432 t
ORIGIN      
        1 cttaaaaaga ttaacatcta catttatcca tagcaaaaaa tcataggatt ttaagaatct
       61 gacaactagg aagaaaacta atatgacctt atctaagaaa caattacaac taagagctaa
      121 aatactggaa acagtttaca cattaggtcc catttcacga attgaaatcg ctactaaaac
      181 aggaataaca cctgcaacaa ctagcagtat cacaaatgat ctgattaaag aaaatattct
      241 cttagaactt ggtgaagacg aacacgatac aagcgtaggg agaaaaaaaa tcctgctaga
      301 cattcaagct aaacgatttt actacattgg ttgtgaacta tctgaaaaac atttcacttt
      361 tgctttagga gataatctag gaaatatctt aaaggaagag aaagaaattg ttactaaaca
      421 gttaatccaa gaaaagggaa atcaactcat taaccagaca ctaaagcaat tcttaaataa
      481 ctgctctgat tatgaaatag aagcaattgg aatcgcgcta cctggtcgct atttagataa
      541 ttataaaata acaacaaaca atcctttatg gcaacatatc gacttagaaa tgattcaatc
      601 ccactttgat aagcctttgt ttttctctaa caatgttaac tgtatggcta taggtaaacg
      661 cctatttagt cgccaacaga atgaccctaa ttttgcttat tttcattttg ctagaggtat
      721 gcactgttct tatatctacg acggaaacat ttatgggaaa ggaaatctaa tgattggaga
      781 aatagggcat acagttgttt catctgaggg agaagaatgt agctgcgggc gcaagggatg
      841 cttacaaact tttgctggag aatcttggtt aatcaaaaaa tcaaaaattc tatatcatca
      901 atctccctat tctctacttc ctagtttagt caaaaatgcc gatgatattg atatccaagt
      961 tattttaacc gcttatcaat taggtgacac tggaataatc acacttatcc atcaagcctt
     1021 actctatctt agtcaaacca ttttaaatat cagtatgatg attgactctc aaaaaatcta
     1081 cctacatagt cctttactaa caaatcaaca catcatccaa aaactatata gtgaaatgaa
     1141 ctacaaaccc aaactgctct ataatcgcct acctgaagtc attattgaac cttacaatga
     1201 ttttacagcc gcacactcag ctattgcact ttgtttgtat catacaattc tacattctta
     1261 attttctatt acatttaata aataggctaa aaagagggag tatatcataa aaagcgaata
     1321 aacctgtttt ctgaaaatca gaattctgtt ttgttcgctt tctttatatt tggaattata
     1381 ctcctatctc ccctttttca taatgtata
//
LOCUS       DAVLYV010000169         1405 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLYV010000169 DAVLYV010000000
VERSION     DAVLYV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.169"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            58..919
                     /locus_tag="VYO70_002147"
                     /pseudo
     CDS             58..919
                     /locus_tag="VYO70_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(954..1163)
                     /locus_tag="VYO70_002148"
                     /pseudo
     CDS             complement(954..1163)
                     /locus_tag="VYO70_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1329..1402)
                     /locus_tag="VYO70_002149"
     tRNA            complement(1329..1402)
                     /locus_tag="VYO70_002149"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1366..1368),aa:Asn,seq:gtt)
BASE COUNT          463 a          281 c          226 g          435 t
ORIGIN      
        1 ctataatatt tttatatagg tattatggtt ctgattttct agtataaaaa aattcaaact
       61 agaattcttc ttagaaaagg ccgttttaag tgctatcatt gctcaaaaat ggagctcgct
      121 gagacttcta tcgtcaagaa gaatcatcaa attccttgta tcatcaacca aaagattgct
      181 cagaagctaa ttgaaaagac ttctatgacc gacattgatc atcagttgtc tatttcaact
      241 tcaactgtca ttcgcaagat caatgatttt cactttgagc atgatttttc gcgtcttcct
      301 gagattatgt cctagtacgt tgaaacagtc cagggagtga ctatttcaat cgggagatgg
      361 agatgagctt tattgctcag gactttgata agctcaatat cataactgtt cttgagagca
      421 gaacacaagc catcatccga aatcccatgg atacaaggct atcaagcgct actggaaact
      481 cattcaacag gatagtcgta aactgagtga taaacatttt tatcgtccta cttttcgtat
      541 gcacttaacc aataaagaga ttttaaacaa gcttttgagt tattcggaag acttgaaaca
      601 ccactatcaa ctctatcagc tcttgctttt tcacttccag aataaggaac cagagaaatt
      661 tttcggactt attgaggaca atcttaagca ggttcatcct atttttcaga ctgtctttaa
      721 aaccttcctc aaagataaag aaaagattgt caacgccctt caattacctt attccaacgc
      781 caaactggaa gccaccaata atctcatcaa acttatcaag cacaatgcct ttggttttag
      841 gaactttgaa aacttcaaaa aagaaaggac gaaatttgtc ctttctaaat cttcgctttc
      901 ttcaacccac tacagttgac aaagaaccga aaaatgtctc gcctctaata tagtcaattg
      961 aaacaagaac aagacaaaag agcctcgtaa aaggtattgc aacttggtaa tacctttttg
     1021 aggtgctttt tgatatgagc ccatgttttc ttaataggat tgtactcagg tgagtaggga
     1081 ggaagaggta aaagttcatg cccaaactcc tcgcataaaa gttctagctt acccattcta
     1141 tggaatcttg cattgtccat aatatagtag attgaaacta gaatagtaca cctctacttc
     1201 taaaacattg ttagaattcg attttactgt cctgatcgat ttgtcctatt cttatttcat
     1261 tttactataa cacgaaatag attattatta cataacaaaa aagaggtcta aacctcttaa
     1321 ctaaattact ccgccagtag gactcgaacc tacgacatca tgattaacag tcatgcgcta
     1381 ctaccaactg agctatggcg gatta
//
LOCUS       DAVLYV010000170         1300 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLYV010000170 DAVLYV010000000
VERSION     DAVLYV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.170"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(82..201)
                     /locus_tag="VYO70_002150"
     CDS             complement(82..201)
                     /locus_tag="VYO70_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1714285.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            459..596
                     /locus_tag="VYO70_002151"
     CDS             459..596
                     /locus_tag="VYO70_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714286.1"
                     /translation="MNPDRAEEYFCRGCQGENPEDIEFYDEQLQAEKVEVLNIRLEVK
                     N"
     gene            689..1300
                     /locus_tag="VYO70_002152"
                     /pseudo
     CDS             689..1300
                     /locus_tag="VYO70_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT          461 a          196 c          222 g          421 t
ORIGIN      
        1 aaagattgcc tgtgggtaaa cagtcacaca gcagtctaag ccatcaaata cctataaccc
       61 ttaaaatagc gatttcggtc gctatttatt tttcgaaaaa tcctcttgac tcttttgcga
      121 attagattta tcatcagacg tgtagaagaa aatcaaggag tcactatgga aaaattcaat
      181 ttcaaaaaca atatcgggca agaaaacaag ttacttcaaa tcgaaatcta caagtttact
      241 aacttttgca agcttcaaaa ttacacatct gtaaatattt tttcgaaaga tatatttgaa
      301 gccatagtaa attgagacta gaatagtaca ctacatctgc taaaaatttc tagaaactaa
      361 tttaactttc ctaatcgatt tgtttacatc ttatttcaat ctactatatt atctcaataa
      421 atgatgatgg cgactgtttt gtttgtgctt gtcgattttt gaatcctgat cgagcggaag
      481 aatatttctg ccgaggttgc caaggggaaa atcctgaaga tattgaattt tatgatgaac
      541 aattacaggc agaaaaggtg gaggttttga atattcgatt ggaagtaaaa aattgaatta
      601 tctacaatct catcaacaat aaacctattt caaccccatt atctataggc gtatttttac
      661 gcctttttat atacttgaag cacataagaa acattatggg aagtttgctg atggaagcac
      721 tcaagaaatt gatgttcctt atgatattcc tgatacttgg gagtgggtga ggttttctac
      781 attggttgaa attgtcagag gtggctctcc acgaccaatc aaagattatc ttacttctga
      841 agtagatgga ataaattgga taaaaatagg tgatactgaa aagggtgaaa agtatataaa
      901 taatgttaaa gaaaaaatca aaaaatcagg gcttaacaaa actagatttg taaaaaaagg
      961 tacatttttg ttaactaatt ctatgagttt tggtagacct tatattttga atgttgatgg
     1021 tgcaatacac gatggatggt tggctatttc gaactatgaa aactcattaa ataaagatta
     1081 cctattctat attctttcat caaatgtagt ttattctcaa tttctatctc taattagtgg
     1141 agctgttgtg aaaaacttga atagtgataa agttgcttct attcttatcc ctctccctcc
     1201 gctagctgaa caacaacgca taatagaagc aatcgaatca gctttagaaa aagtagatga
     1261 atatgctgaa agttataata gactagaaca gctagataaa
//
LOCUS       DAVLYV010000171         1249 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLYV010000171 DAVLYV010000000
VERSION     DAVLYV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.171"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>1249
                     /locus_tag="VYO70_002153"
     CDS             <1..>1249
                     /locus_tag="VYO70_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEV1714287.1"
                     /translation="SNVILTGTGGIGKSMLVKHIFINQVQQATSIPIFIELKSLNESD
                     FSENELVDFIYQEVQNHHLNLEKKYFKATLEAGRYTIIFDGLDEVNPSKRSWLDKEIK
                     EFVTLYNSNRYVLSSRPSEEFIGWNQFAEYEIKKLNREQALSLIDKLNYDEKVKRRFY
                     RELKDHLYDTHESFASIPLLLTIMLMTYEAGASIPNNLTDFYNQAFYTLYQRHDASKS
                     GYKRELKAKLTPEEFRNILAYIGLKTFFEGKVDFDRTTLDEIITKYCFKNNLELKTND
                     IVYDATHSACMMLQEGVSLKFSHRSFQEYFAAVGVNQLDDKLQKQILIKWSEADRNNI
                     VAHKTFMNALFKIQKERTYKNLCIPIIERMDEKYSRMGDITEKIVTCFNYFICRKDSR
                     ENKLELHFLLTKEVHFYFSLQFLI"
BASE COUNT          473 a          158 c          208 g          410 t
ORIGIN      
        1 tctaacgtta ttttgacagg aacgggtggg attggtaaat caatgcttgt aaagcatatt
       61 tttatcaatc aagtacaaca agctacatca attccaattt ttattgaatt aaaatcactt
      121 aatgaatctg atttctcaga aaatgaatta gttgatttta tttaccaaga ggttcaaaat
      181 catcatttaa atttagaaaa gaaatatttt aaagcgacat tagaggctgg aagatataca
      241 ataatttttg atggtttaga tgaggttaat ccttcaaaac gttcttggtt ggacaaagaa
      301 attaaagaat ttgtaacgtt atacaatagc aatagatatg ttttgtcgtc aagaccgtct
      361 gaggaattta ttggttggaa tcagtttgct gagtatgaga taaaaaaatt aaatagagag
      421 caagcgttat ccttaattga taagttgaat tatgatgaga aagtaaaaag gcgtttctac
      481 agagaattaa aggatcattt atatgatact catgaatctt ttgcttctat cccactccta
      541 ttaacaatta tgttaatgac ttacgaagct ggtgcttcta ttccaaataa tttaactgat
      601 ttttataatc aggcctttta tacattgtac caaagacatg atgcatctaa atcaggatac
      661 aaaagagaac taaaagcaaa attaactcct gaagaatttc gaaatatatt agcctacatt
      721 ggtttaaaaa cattctttga aggaaaagta gatttcgata gaactacact tgatgaaata
      781 attactaaat attgttttaa aaataacctt gaattaaaga cgaatgatat agtttatgat
      841 gcgactcata gtgcttgtat gatgcttcaa gaaggcgtta gtttaaaatt ctctcaccgt
      901 tcattccaag aatactttgc tgcagtaggt gttaatcagc tagatgataa actccaaaaa
      961 cagatactaa ttaagtggtc tgaagcagat aggaataata ttgtagcaca taaaactttt
     1021 atgaatgcac tttttaaaat acagaaagaa aggacttaca aaaatttatg tattccaatc
     1081 atagaaagaa tggatgaaaa gtatagcaga atgggggata tcacagagaa aattgttacg
     1141 tgtttcaatt actttatctg tagaaaagat agtagagaaa acaaattaga attgcacttt
     1201 ttactaacaa aggaagttca cttttatttt agcttgcaat ttttaatat
//
LOCUS       DAVLYV010000172         1186 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLYV010000172 DAVLYV010000000
VERSION     DAVLYV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.172"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>1186
                     /gene="phtD"
                     /locus_tag="VYO70_002154"
     CDS             <1..>1186
                     /gene="phtD"
                     /locus_tag="VYO70_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtD"
                     /protein_id="HEV1714288.1"
                     /translation="LIFDPAQITSRTARGVAVPHGNHYHFIPYSQMSELEERIARIIP
                     LRYRSNHWVPDSRPEQPSPQSTPEPSPSPQSAPNPQPAPSNPIDEKLVKEAVRKVGDG
                     YVFEKNGVSRYIPAKNLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAY
                     DLLARIHQDLLDNKGRQVDFEALDNLLERLKDVPSDKVKLVDDILAFLAPIRHPERLG
                     KPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDS
                     LSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNL
                     QYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERP
                     HS"
BASE COUNT          379 a          262 c          246 g          299 t
ORIGIN      
        1 gccttatttt cgacccagcg caaatcacaa gtcgaaccgc cagaggtgta gctgtccctc
       61 atggtaacca ttaccacttt atcccttact ctcaaatgtc tgaattggaa gaacgaattg
      121 ctcgtattat tccccttcgt tatcgttcaa accattgggt accagattca agaccagaac
      181 aaccaagtcc acaatcgact ccagaaccta gtccaagtcc gcaatctgca ccaaatcctc
      241 aaccagctcc aagcaatcca attgatgaga aattggtcaa agaagctgtt cgaaaagtag
      301 gcgatggtta tgtctttgag aagaatggag tttctcgtta tatcccagcc aagaatcttt
      361 cagcagaaac agcagcaggc attgatagca aactggccaa gcaggaaagt ttatctcata
      421 agctaggagc taagaaaact gacctcccat ctagtgatcg agaattttac aataaggctt
      481 atgacttact agcaagaatt caccaagatt tacttgataa taaaggtcga caagttgatt
      541 ttgaggcttt ggataacctg ttggaacgac tcaaggatgt cccaagtgat aaagtcaagt
      601 tagtggatga tattcttgcc ttcttagctc cgattcgtca tccagaacgt ttaggaaaac
      661 caaatgcgca aattacctac actgatgatg agattcaagt agccaagttg gcaggcaagt
      721 acacaacaga agacggttat atctttgatc ctcgtgatat aaccagtgat gagggggatg
      781 cctatgtaac tccacatatg acccatagcc actggattaa aaaagatagt ttgtctgaag
      841 ctgagagagc ggcagcccag gcttatgcta aagagaaagg tttgacccct ccttcgacag
      901 accatcagga ttcaggaaat actgaggcaa aaggagcaga agctatctac aaccgcgtga
      961 aagcagctaa gaaggtgcca cttgatcgta tgccttacaa tcttcaatat actgtagaag
     1021 tcaaaaacgg tagtttaatc atacctcatt atgaccatta ccataacatc aaatttgagt
     1081 ggtttgacga aggcctttat gaggcaccta aggggtatac tcttgaggat cttttggcga
     1141 ctgtcaagta ctatgtcgaa catccaaacg aacgtccgca ttcaga
//
LOCUS       DAVLYV010000173         1186 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLYV010000173 DAVLYV010000000
VERSION     DAVLYV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.173"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            8..856
                     /gene="dprA"
                     /locus_tag="VYO70_002155"
     CDS             8..856
                     /gene="dprA"
                     /locus_tag="VYO70_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1714289.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
BASE COUNT          354 a          209 c          259 g          364 t
ORIGIN      
        1 gttatttatg aaaatcacaa actatgaaat ctataagtta aaaaaatcag gtttgaccaa
       61 tcaacagatt ttgaaagtgc tagaatacgg tgaaaatgtt gatcaggagc ttttgttggg
      121 tgatattgca gatatatcag gttgccgtaa tccagccgtt tttatggaac gttattttca
      181 gatagacgat gcgcatttgt cgaaagagtt tcaaaaattt ccatctttct ctattttaga
      241 tgactgttat ccttgggatt tgagtgaaat atatgatgcg cctgtacttt tattttacaa
      301 gggaaatctt gacctcctga aattcccgaa ggtagcggtc gtgggcagtc gtgcttgtag
      361 caaacaggga gctaagtcag ttgaaaaagt cattcaaggc ttggaaaatg aactggttat
      421 tgtcagtggt ctggccaagg gcattgacac agcagctcat atggcagctc ttcagaatgg
      481 cggaaaaacc attgcagtga ttggaacagg actggatgtg ttttatccta aagccaataa
      541 acgcttgcaa gactacatcg gcaatgacca tctggttcta agtgaatatg gacctggcga
      601 acaacctctg aaatttcatt ttcctgcccg taatcgcatc attgctggac tttgtcgtgg
      661 tgtgattgta gcagaggcta agatgcgttc aggtagtctc attacgtgtg agcgagcaat
      721 ggaagaagga cgcgatgtct ttgctattcc tggtagcatt ttagatggac tatcagacgg
      781 ttgccatcat ttgattcaag aaggagcaaa attggtcacc agtgggcaag atgttcttgc
      841 ggaatttgaa ttttaaaaat gacctaagct agaattctaa gaaaaaatca attttaagag
      901 aaaatgaacc caacatttcc ataataaaac gcatattagc aagtttttaa cacttgctaa
      961 tatgcgtttt ttctaagtgg attagtagag tagaggattt ttctcatata atactcttcg
     1021 aaaatctctt caaaccacgt cagcttccat ctgcaacctc aaaacagtat tttgagctga
     1081 cttcgtcagt cttatctaca acctcaaagc agtgctttga gcaacctgtg gctagcttcc
     1141 tagtttgcgc tttgcttttc attgagtata agggaaagta tagtga
//
LOCUS       DAVLYV010000174         1185 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLYV010000174 DAVLYV010000000
VERSION     DAVLYV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.174"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>1185
                     /locus_tag="VYO70_002156"
     CDS             <1..>1185
                     /locus_tag="VYO70_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S74"
                     /protein_id="HEV1714290.1"
                     /translation="KADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGS
                     IRQEILNATKDKASKSELTQTAEELSSKIASVQVGGINLLRNTASLLIGDRSKGCWMS
                     ASGGNGRAISVEVLDPPKKMIKNMIRVIENTNGGNKDLTQLVRLRIGEKYTISCYARI
                     ASDSPNANVNLLFRSWANNTDLNRKFQKSISHKNWQKYSFTFTADAIENSIQFGQSGA
                     GIIEICAPKIESGTLATDYSEAPEDIEGQISTVESTFKQRANSLDAGVSRLTEGLRTK
                     VDISALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKADK
                     TLVVSEAGKLREEFSKMKVGGRNLWIKSKTVGAVIEKLPENHVTGQKECYRLENNSTL
                     MFN"
BASE COUNT          405 a          216 c          279 g          285 t
ORIGIN      
        1 aaagccgata tcagctcact caatgtgact gctgaaaata tccggcaatc tgtgaagagt
       61 cttgagacag acacgcagaa caaactaaat cagaagttga gtcaggctga atttgaggtg
      121 cgagccggct ctatccgtca ggaaatcctg aacgcaacca aggataaagc cagcaagtca
      181 gaacttacgc agacagctga ggagctctct agtaagatag cgagtgtgca ggtcggagga
      241 atcaacctct tgcgtaatac tgcgagtttg ttgattggcg atcgttcaaa aggatgttgg
      301 atgagtgcaa gcggaggaaa tgggcgagcg attagcgtag aagttttgga tcctcccaaa
      361 aaaatgataa aaaacatgat tcgtgttatt gaaaatacga atggtggaaa taaagattta
      421 actcaacttg ttagattgcg aattggtgaa aagtacacga tttcttgtta tgcaaggatt
      481 gctagcgata gcccaaatgc aaacgtgaac ttgctatttc gttcgtgggc aaataatacc
      541 gatttaaatc gcaaatttca gaaatccatc tctcataaaa attggcaaaa atattcattt
      601 acatttactg ctgatgcaat tgaaaattca attcaatttg gacaatctgg cgcaggaatt
      661 atcgaaatat gcgctccgaa aatcgaatca ggaacgttag cgactgatta cagcgaagct
      721 cctgaagata tagaaggtca gatttcaaca gttgaatcga ccttcaaaca acgagccaac
      781 tcactcgacg ctggtgtaag ccgtctgact gaaggtctta gaactaaagt ggatatcagc
      841 gcactcaacg tgactgctga aaatattagg cagtcggtga agagtcttga gacagacacg
      901 cagaacaaac taaatcagaa gttgagtcag gctgaatttg aggtgcgagc tggctctatc
      961 cgtcaggaaa tcctgaacgc aaccaaggat aaagcagata agactttagt tgtatctgaa
     1021 gctgggaaat tgcgtgaaga attttcaaaa atgaaggtgg gaggacggaa tctatggata
     1081 aaatccaaga cggttggagc tgtaattgaa aaattacctg aaaaccacgt cacaggtcaa
     1141 aaagaatgct ataggctaga gaacaactct actttaatgt tcaac
//
LOCUS       DAVLYV010000175         1128 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLYV010000175 DAVLYV010000000
VERSION     DAVLYV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.175"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            40..>1128
                     /locus_tag="VYO70_002157"
     CDS             40..>1128
                     /locus_tag="VYO70_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate hydrolase"
                     /protein_id="HEV1714291.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKSEAAKKHYEEVKKKAEDAQKKYDEGQKKTVEKAKREKEASEKIAEATKEV
                     QQAYLAYQQASNESQRKEADKKIKEATQRKDEAEAAFATIRTTIVVPEPSELAETKKK
                     AEEAKAEEKVAKRKYDYATLKVALAKKEVEAKELEIEKLQYEISTLEQEVATAQHQVD
                     NLKKLLAGADPDDGTEVIEAKLNKGEAELNAKQAELAKKQTELEKLLDSLDPEGKTQD
                     ELDKEAEEAELDKKADELQNKVADLEKEISNLEILLGGADSEDDTAALQNKLATKKAE
                     LEKTQKELDAALNELGPDGDEEETPAPAP"
BASE COUNT          470 a          168 c          251 g          239 t
ORIGIN      
        1 atagtctaaa aatgactatc agaaaagagg taaatttaga tgaataagaa aaaaatgatt
       61 ttaacaagtc tagccagcgt cgctatctta ggggctggtt ttgttacgtc tcagcctact
      121 tttgtaagag cagaagaagc tcccgtagct agtcagtcta aagctgagaa agactatgat
      181 gcagcagtga aaaaatctga agctgctaag aaacattatg aagaagttaa aaagaaagca
      241 gaagacgctc agaagaaata tgacgagggt cagaagaaaa cggtagaaaa agcaaagcga
      301 gaaaaagaag cttctgaaaa gatagctgag gcaacaaaag aagttcaaca agcgtaccta
      361 gcttatcaac aagctagcaa cgaaagtcag agaaaagagg cagataagaa gataaaagaa
      421 gctacgcaac gcaaagatga ggcggaagct gcatttgcta ctattcgaac aacaattgta
      481 gttcctgaac caagtgagtt agctgagact aagaaaaaag cagaagaagc taaagcagaa
      541 gaaaaagtag ctaagagaaa atatgattat gcaactctaa aggtagcact agcgaagaaa
      601 gaagtagagg ctaaggaact tgaaattgaa aaacttcaat atgaaatttc tactttggaa
      661 caagaagttg ctactgctca acatcaagta gataatttga aaaaacttct tgctggtgcg
      721 gatcctgatg atggcacaga agttatagaa gctaaattaa acaaaggaga agctgagcta
      781 aacgctaaac aagctgagtt agcaaaaaaa caaacagaac ttgaaaaact tcttgacagc
      841 cttgatcctg aaggtaagac tcaggatgaa ttagataaag aagcagaaga agctgagttg
      901 gataaaaaag ctgatgaact tcaaaataaa gttgctgatt tagaaaaaga aattagtaac
      961 cttgaaatat tacttggagg ggctgattct gaagatgata ctgctgctct tcaaaataaa
     1021 ttagctacta aaaaagctga attggaaaaa actcaaaaag aattagatgc agctcttaat
     1081 gagttaggcc ctgatggaga tgaagaagaa actccagcgc cggctcct
//
LOCUS       DAVLYV010000176         1120 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLYV010000176 DAVLYV010000000
VERSION     DAVLYV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.176"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..649)
                     /locus_tag="VYO70_002158"
     CDS             complement(<1..649)
                     /locus_tag="VYO70_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1714292.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEKSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELVLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFILQKKKERLPFVPFLLLATCLIIFGKL"
     gene            719..>1120
                     /locus_tag="VYO70_002159"
     CDS             719..>1120
                     /locus_tag="VYO70_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1714293.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQM"
BASE COUNT          413 a          203 c          262 g          242 t
ORIGIN      
        1 gtagcttacc aaaaataatc aaacaagtag caagtaagag gaaaggcacg aaaggaagtc
       61 tttccttttt cttttgcagg ataaaggcca ggatacccgt cgcagaagca aactgaatca
      121 agatcagtaa ctccgttacg ctaaagacga gagcacaaga agctaaaaag aggaaatctc
      181 ctgcgcccat gcggatatcg ataaaatgag ccaaaattcc aagagcaagg aaggagacca
      241 tgaccagatt ccagccagag gaagctatta ggattaggtg gaaagtcatc cagaccagta
      301 agggatattc ctgatggtga aagtcgtaga tacccaaggt caaaccagcg gtgattagga
      361 cgacttgccc caaggaaagc aattcccaag agtaaagcag aaagaggagt cctaagacta
      421 attcaaagag ggcataccag acaggatagc gaactttgca gtagcgacag cgaaagcgat
      481 tgaagacctg tgagagaatc ggaatcaaat ctaagggacg caagcgagtc tgacaggaat
      541 cgcagtgact ggctgaactg ataatggatt tctctggaaa acggtcaatg accaaaccaa
      601 gaaaggaagc gagaatgctc ccgacgagaa aaaaataaaa atcaatcata cttatctatt
      661 cgtaaaaaat gggagaaata gtataatgga gaaacataga taagatggtg aggaaaagat
      721 gacaattcgt tttgaagaaa aggtgagtat agaaaacgct caatttgtat gccagtggtc
      781 caactcccta ggcaaatcct ttcaagaaca atggatggga ccaaggattc cttttctact
      841 gacccttcaa gccttggaag gagtcttttc aatctttgat gaacaagagt ttgtgggact
      901 tatccagaaa atcaggctag aagacagcaa tcttcatatc gggagatttt ttatcaaccc
      961 ccagaaacag gagcaagact taggtagcca ggctttaagg aaatttgtta gtttggcctt
     1021 tgaaaatgaa gatatagata gtatttctct aaatgtcttc gaggcaaatc aaagagctca
     1081 gaatctttac caaaaagaag gatttgaaat cgttcaaatg
//
LOCUS       DAVLYV010000177         1087 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLYV010000177 DAVLYV010000000
VERSION     DAVLYV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1087
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.177"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            221..>1087
                     /locus_tag="VYO70_002160"
     CDS             221..>1087
                     /locus_tag="VYO70_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1714294.1"
                     /translation="MLYVGIDVAKNKHDVTALNVQGKTVLKPLTFSNNKAGFELLDLS
                     FRQLNQDCLIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLD
                     IIFPELDKIVGKHSEYTYQLLTRYPNPQKRLEARFDKLIEIKRLTASKIQDILSIAPR
                     SIGTTSPAREFEIIEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGGRLGAVILA
                     EIRNIHAFDNPAQLQAFAGLDSSIYQSGQIDLAGRMIKRGSPHLRWALIQAAKACARF
                     SPAFKAYLKTKLE"
BASE COUNT          324 a          233 c          211 g          319 t
ORIGIN      
        1 tactgccaaa gcccaagtgg tatacttgga ataagccact gtggattagt tcatttcctc
       61 caatcgcttg acgatttccg aaagggtcta accacagctt tttgtttcaa ttggtaatga
      121 gtttgataac gcatagacgt tttatctcgt ctaaggtaac aaggcaaaaa gttcgtggat
      181 aaccaagtag acttgaaata ctatatctgg aggtactccc atgctttatg tgggcattga
      241 tgtcgctaaa aataaacacg atgttacagc cttgaatgtt caaggaaaaa ctgttcttaa
      301 accactcact ttttcaaata ataaagctgg ttttgaactc ttagatctgt cttttcgaca
      361 gctcaaccaa gactgtctca tcgctcttaa acttctttct gaccccaatc gtgaacaatt
      421 tcaacacgat aatcggcaag tagaactaaa aatactggct agacatattc atcgtctcaa
      481 gaaaaaacag tctgattgga aagtacaata cactcgttgt cttgatatca tctttcctga
      541 gttggataaa atcgttggaa agcattcaga atatacctac caactcttga cacgctaccc
      601 taatcctcag aaaaggcttg aggcaagatt tgataagctg atagaaatta agcgattgac
      661 cgcttctaaa attcaggata tcctctcaat cgcacctcgt tctatcggaa caacatctcc
      721 tgctcgtgaa ttcgaaatca tcgaaatcat caaacattac aagaggctca ttgacaaggc
      781 ggaaacatgt gtcaatgact tgatggctga gttcaactcg gtcatcacga cggttactgg
      841 gattgggggt cgtttagggg cggtcatttt agccgagatt cgaaatattc atgcctttga
      901 taatcctgct caattacaag ctttcgctgg actggattct tctatttatc agtcaggtca
      961 gattgattta gctggaagaa tgatcaaacg gggttcccct catctgcggt gggcactcat
     1021 acaagctgcc aaagcatgcg ctcgcttttc acctgctttt aaggcctatc ttaagactaa
     1081 gttagaa
//
LOCUS       DAVLYV010000178         1036 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLYV010000178 DAVLYV010000000
VERSION     DAVLYV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.178"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..185)
                     /locus_tag="VYO70_002161"
     CDS             complement(<1..185)
                     /locus_tag="VYO70_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1714295.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGILP
                     SKNEELMKFFCENNLNIT"
     gene            complement(198..947)
                     /locus_tag="VYO70_002162"
     CDS             complement(198..947)
                     /locus_tag="VYO70_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEV1714296.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCSDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
BASE COUNT          359 a          198 c          115 g          364 t
ORIGIN      
        1 gtaatattta agttattctc gcaaaaaaac ttcataagct cttcattttt agatggaaga
       61 atgccctttt ttaaattatt tatttttaca gaatctatat catatgcaac aactttatat
      121 ttagatgcaa atagtaacgc gtaggccagc ccaacatgcc ccaaaccaat tactgctata
      181 ttcataaaac tacttcctta tttcttaatc caaaatctaa tagaataagc tgccccattc
      241 cttaaataca actctttaat attgtttaaa agtttttcaa ctgatttcca gattatcaaa
      301 atctgagatt tatagcacaa tattgatgat attctatcaa tataatgttt ttcatcaagt
      361 tcctcttgat acatttttaa ttctttagtt tttcccatat aactaaccat actactatca
      421 cttacatatg ggaagtcctc ataatatatt actttataac gcataaattc aagcgccctt
      481 ccaatactat tcacaaaaac atgatcaaca tggtcaccaa gtgaaagcgg acaatatacg
      541 acacatttat cgtctaaatg cattaacagc tcttttatga tatcattctt taatgtgtcc
      601 tcatttttta attcactata gatatgacgg tatagaaaat tgccatttct atctttccta
      661 tagagacatt catagtacga taagtgtcta aaatcacatt gtagacgttc acaagctaac
      721 ctgtcttctt tcttcctttc ttcaatcgga tatttcccaa ggttacacaa cttatgaaat
      781 tgcttagcag agggctgtag ctgttggctc aaagggtaac cagaaaatat agtaataaca
      841 agtacaattt ctccttctga agttaatttt gaaatataat cactacagga aaaaattgcg
      901 tcatctaaat gtggagataa aaagatatac ttagtattgt tactcataac cattccctct
      961 acaatttatc taaaaactca ctaagtgtct gattaaattc cacatcatca aaaaaattca
     1021 ccttattctt aataat
//
LOCUS       DAVLYV010000179          990 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLYV010000179 DAVLYV010000000
VERSION     DAVLYV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.179"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..967
                     /locus_tag="VYO70_002163"
     CDS             <1..967
                     /locus_tag="VYO70_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1714297.1"
                     /translation="SSNSVKNEENTSKEHAPDKIVLDHAFGQTILDKKPERVATIAWG
                     NHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKELNGKANLFDDLDGLNFEAIS
                     NSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQTLWRDMIKIDSKALGMEKEGD
                     ELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAADTSKFWIYTSKDPRANYLTDL
                     GLVFPESLKEFESEDSFAKEISAEEANKINDADVIITYGDDKTLEALQKDPLLGKINA
                     IKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLGNACKNAK"
BASE COUNT          398 a          142 c          168 g          282 t
ORIGIN      
        1 ttcttctaat tctgttaaaa atgaagaaaa tacttctaaa gagcatgcgc ctgataaaat
       61 agttttagat catgctttcg gtcaaactat attagataaa aaacctgaaa gagttgcaac
      121 tattgcttgg ggaaatcatg atgtagcatt agctttagga atagttcctg ttggattttc
      181 aaaagcaaat tacggtgtaa gtgctgataa aggagtttta ccatggacag aagaaaaaat
      241 caaagaacta aatggtaaag ctaacctatt tgacgatttg gatggactta actttgaagc
      301 aatatcaaat tctaaaccag atgttatctt agcaggttat tctggtataa ctaaagaaga
      361 ttatgacact ctatcaaaaa ttgctcctgt agcagcatac aaatctaaac cttggcaaac
      421 tttatggaga gatatgatta aaattgattc aaaagcctta ggtatggaaa aagaaggtga
      481 tgagttaatc aaaaatactg aagctcgtat atccaaagaa ttagaaaaac atccagaaat
      541 caaaggaaaa atcaaaggaa aaaaagtatt atttactatg attaatgctg cagatacatc
      601 aaaattctgg atttatacta gcaaagatcc aagagcaaat tatttaacag atttaggtct
      661 agttttccct gaatcattaa aagaatttga gagtgaagat agttttgcaa aggaaatttc
      721 tgcagaagaa gcaaataaga taaatgatgc tgatgtaatc ataacttatg gtgatgataa
      781 aactcttgaa gctttacaaa aagatcctct tttaggtaaa ataaatgcaa ttaaaaatgg
      841 tgccgttgct gtaattccag ataatacacc gttagcagcc tcatgcactc caacaccact
      901 ttcaataaac tatactattg aagaatacct aaatctttta ggaaatgcat gcaaaaatgc
      961 gaaataaaaa acaaataaac ctaggcataa
//
LOCUS       DAVLYV010000180          953 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLYV010000180 DAVLYV010000000
VERSION     DAVLYV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..953
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.180"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            335..>953
                     /locus_tag="VYO70_002164"
     CDS             335..>953
                     /locus_tag="VYO70_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1714298.1"
                     /translation="MEELFIIEDISVESSFYLGKFGVMYTRSKEYGRPSKLFYKSFDS
                     FTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFDRTGFVRHSYTETVA
                     PTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSITIPYQDRARRVHCIG
                     RYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYENSF"
BASE COUNT          316 a          137 c          186 g          314 t
ORIGIN      
        1 gaggtctatc tcttcatcat ttcaggaatt aaaaaaatgg aattctttga tatttaggag
       61 agggcgatat tggtaccaat ttgataattg gagtataccc aatactggtg taataaagga
      121 atggtatgtc gaagataatg actggattcc taaggttagt tcttccagtt tagacaattc
      181 aaattcactt aaaattgtct ttgatgtgga aaaatatact ttgccagatg atagtatctt
      241 acaggatgtt aggatttcat ctgatgagag ttatatagtt ttggttgtat cctcactgcg
      301 tagcactaat ttaattggaa taaaaaaata tactatggaa gagttattta tcattgaaga
      361 tatttcagta gaaagttcgt tctatttggg taaattcggt gttatgtata ctcgctccaa
      421 agagtacggg aggccaagta agttattcta taaatctttt gatagcttta cagaagagga
      481 actgtttgaa gaaaacgaat gctcttttcg attaaaaata gtccacattg atagcaataa
      541 ttgttttgta aagtcagtag actttcagaa aggcaggatt tttctataca gtttcgaccg
      601 gacgggtttt gttagacact cttacacaga aacggtagcc ccaacaccta gagatatcgc
      661 tctatttagt acagaaaaag cacagtactt tcttgggctc tcttcaactg aagaaaagaa
      721 agatcaacta attctaaggg aaacttcttc gggagacaga gtttctatta ctataccata
      781 tcaagatagg gctagaagag ttcactgtat aggacgttat atccttttag actgttcaaa
      841 cgcagcgaat tccgtctttt atcttgctac atttaaaaat aatagtttga gggagttgag
      901 tgttaataag ataacatttg atgaaaagac aacactttat gaaaattcat ttt
//
LOCUS       DAVLYV010000181          932 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLYV010000181 DAVLYV010000000
VERSION     DAVLYV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.181"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..441
                     /locus_tag="VYO70_002165"
     CDS             <1..441
                     /locus_tag="VYO70_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714299.1"
                     /translation="FLFNQNKWEKEDSFESDLTNTLKDKIQKSDLSYSDLMSLQLYDA
                     MNQSTFLTDSELFEDILSKIEDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQ
                     LIRINQLDFFYYTSWLRQNFDFCMNREYHKMKSRKLLLSNKKIV"
     gene            497..850
                     /locus_tag="VYO70_002166"
     CDS             497..850
                     /locus_tag="VYO70_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1714300.1"
                     /translation="MDLVLPNNYVVIDEEEMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
BASE COUNT          321 a          119 c          192 g          300 t
ORIGIN      
        1 tttcttttca atcaaaataa atgggaaaaa gaagattcgt ttgaaagtga tttgacaaat
       61 acgttaaagg ataaaataca aaaatcagat ctcagttatt ctgacttaat gtctcttcag
      121 ctgtatgatg cgatgaacca atccaccttt ttaacggata gtgaactctt tgaggatatt
      181 ctgagtaaaa ttgaagattc tcataacacc cttctgtgta caggtcttgt cgagttactg
      241 ctgtatgaga tttctattag taacaataac aactggcaag aaaaggttga taaaattaga
      301 atacagctca tcagaataaa tcaattagat tttttctatt atacaagttg gttaaggcaa
      361 aattttgatt tctgtatgaa tagagagtat cataaaatga aatctcggaa attattactg
      421 agtaataaaa agatagttta agttcgtcct atttgtaaaa caaatgaaac aatttcaact
      481 aaggaggaga aaacttatgg acttagtatt accaaataat tatgttgtga ttgatgaaga
      541 agaaatgatg tatcttgatg ggggagctta tttaagcaag cgtgcttgtc aaggaatttg
      601 cgcagcttta gctatgagtt caggaacttt tatagcatta gctggagctg cagttttaac
      661 caaaaaacta ataaactata ttaaggttgg aggtcttgga ggctggctta ttggtgcagc
      721 agcaggtgta ttggctacag cagcagggaa aatagcttac tgtattggat atggtgttct
      781 taatagaggt tgtgatatta acgggaaccc ttatccttgg gatggattca tatctgcaac
      841 agtaagatga gtaatgtaga taagattcga aaaatacaca tcatcgtgtg ttgggtgtat
      901 atatttttat cattcagggc gattataaat ga
//
LOCUS       DAVLYV010000182          912 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLYV010000182 DAVLYV010000000
VERSION     DAVLYV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.182"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>912
                     /locus_tag="VYO70_002167"
     CDS             <1..>912
                     /locus_tag="VYO70_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HEV1714301.1"
                     /translation="AKLDYDAPSCPDCGSLMKKYDFQKPSKIPYLETTGMPSRILLRK
                     RRFKCYHCSKMMVAETPLVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLAISTSTVI
                     RKLNDFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNH
                     FLRYDRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQI
                     MNQFERKSHEYKAIKRYWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDL
                     KHHYQIYQLLLFHFQNKDPEKFFGLIED"
BASE COUNT          282 a          199 c          152 g          279 t
ORIGIN      
        1 gccaaactgg actacgacgc cccatcttgc cctgattgtg gaagtctaat gaagaaatat
       61 gactttcaaa aaccgtctaa gatcccttac ctcgaaacaa ctggtatgcc ttctagaatt
      121 ctccttagaa aacgccgttt caagtgctat cactgttcaa aaatgatggt tgctgagact
      181 cccctggtaa agaaaaatca ccaaatccct cgtatcatca accaaaaaat tgctcaaaag
      241 ttaattgaaa aaatttctat gactgatatt gcccatcagc tggccatttc aacttcaact
      301 gtcattcgca agctcaatga ctttcacttt gagcatgatt tttctcggct tccaaagatt
      361 atgtcttggg atgagtatgc cttcactaag ggaaagatga gtttcattgc gcaagatttt
      421 gataatctta atattatcac tgttcttgaa ggcagaacac aagctgtcat ccgaaatcac
      481 tttcttcgct acgatagagc cgttcgttgt caagtgaaaa tcattacgat ggatatgttt
      541 agtccttact atgacttggc taaacagctt tttccgtgtg ctaaaatcgt tctagatcgt
      601 ttccatatta tccaacatct cagccgtgcc atgagtcgtt ttcgtgttca aattatgaat
      661 cagtttgaac gaaaatctca tgaatacaag gctatcaagc gttactggaa actcatccaa
      721 caggatagtc gtaaattgag cgataaacgt ttttatcgcc ctacttttcg catgcactta
      781 acaaataaag aaattcttga caagatttta agctattcag aagacttgaa acaccactat
      841 cagatctatc aactcttact ttttcacttt cagaacaaag accctgagaa atttttcgga
      901 ctcattgagg ac
//
LOCUS       DAVLYV010000183          866 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLYV010000183 DAVLYV010000000
VERSION     DAVLYV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..866
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.183"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..296
                     /locus_tag="VYO70_002168"
                     /pseudo
     CDS             1..296
                     /locus_tag="VYO70_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
     gene            350..586
                     /locus_tag="VYO70_002169"
     CDS             350..586
                     /locus_tag="VYO70_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714302.1"
                     /translation="MNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVKQKL
                     PKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            570..866
                     /locus_tag="VYO70_002170"
     CDS             570..866
                     /locus_tag="VYO70_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714303.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
BASE COUNT          372 a           86 c          161 g          247 t
ORIGIN      
        1 tgataattta gtagtctcag agaatagtat attcgggggt aaagaaggaa ttgttattga
       61 ggattcaaag ggtaaaataa ccgttttaaa taaccaattt tataatttat ccggtaagta
      121 tatatcattc atcaaatcta atgcaaatgg gaaagaacct gttatacgtg atagcgatgg
      181 taatttcaat attgtaacgg agaatgggct ttacaatatt gtaacaaata atttaagtga
      241 taaaaacgaa aaagaaaaaa acaaagagga aaaacaatct aattcaaata atgtaattga
      301 tagtaaccag aagaacggag agtttaactc aagtaaagat aatagacaaa tgaatgacaa
      361 gatcgacaat aaacaagata ataagacaga agaagtaaac tataaaatag ttggagatgg
      421 cagagaaact gaaaatcata ttaataaatc taaagaaata gtagatgtaa aacaaaaatt
      481 accaaagact ggttcgaaca agattatgga actattctta acagtgacag gaattggttt
      541 acttttgaca ctaaaagggt tgaagtatta tggtaaagat aaataaaatt tgttcgatac
      601 aaggaagttc cgtagaaaat gaggatattg taggttcaca aaatcagtat ttttggatta
      661 ttggtggagc tacagattta tataattcta aagaggagat aggttattca gtctcagaag
      721 ttgtacatat tttatcagaa agtttgtcgg ttaattgtaa agaatcaaaa actcttaaac
      781 aaatatttga aactgcttta ctcgaggtta aagatgaaat cggtttaaac tcatatgaat
      841 taacagagta tagtaaaatg aaataa
//
LOCUS       DAVLYV010000184          865 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLYV010000184 DAVLYV010000000
VERSION     DAVLYV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.184"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..865
                     /locus_tag="VYO70_002171"
                     /pseudo
     CDS             1..865
                     /locus_tag="VYO70_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          333 a          138 c          170 g          224 t
ORIGIN      
        1 aaatgggacg taagccaaaa aagagacctg aagagaggac agaattagaa cgtcttcaag
       61 cagaaaatga gtacctgaga gcggagaatg ccatcctaaa aaagttaaga gaactccgat
      121 tgaaggagga aaaagagaaa gaagaaagac agaaattgtt caagaattaa tgactgagtt
      181 ttcgttagat cttcttctaa aagccattaa actagctcgt tggacctact actatcactt
      241 gaaacagcta gacaaaacag ataaggacca agagcttaaa gctgaaattc aatccatttt
      301 tatcgaacac aagggaaatt atggttatcg tcgggttcat ttagaactaa gaaatcgtgg
      361 ttatctggta aatcataaaa gagttcaagg cttgatgaaa gtactcaatt tacaagctaa
      421 aatgcgacag aaacgaaaat attcttctca taaaggagac gttggtaaga aggcagagaa
      481 tctcattcaa ggccaatttg aaggctctaa aacaatggaa aagtgctaca cagatgtgac
      541 tgaatttgcc attccagcaa gtactcaaaa gctttactta tcaccagttt tagatggctt
      601 taacagcgaa attattgctt ttaatctttc ttgttcgtct aatttagaac aagtacaaac
      661 aatgttggaa caggcattca cagagaagca ctatggtgga gtccttcttt gggattctga
      721 aatcggaaat gttttatggc tatgagaaaa catttaaatc acttaatcaa ttggaacaag
      781 ctattataga ctatattgat tattacaaca acaagagaat taaggtaaaa ctaaaaggac
      841 ttagccctgt gcaatacaga actaa
//
LOCUS       DAVLYV010000185          863 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLYV010000185 DAVLYV010000000
VERSION     DAVLYV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.185"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..842)
                     /locus_tag="VYO70_002172"
     CDS             complement(<1..842)
                     /locus_tag="VYO70_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1714304.1"
                     /translation="MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNP
                     IADPGILGINTGASLSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGL
                     TPIKLALSGTCVSMALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFII
                     LGHLIAIFISSDLNALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFV
                     GLIVPHFCGLFISKDIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGG
                     PVLI"
BASE COUNT          312 a          168 c          130 g          253 t
ORIGIN      
        1 taaataagta ctggaccccc gattattgca gtaattatcc ctacttcaat ttcacctggt
       61 ttacctaaca tacggccgat tatatcacat ataagcaaga gctctgcacc tataaaagat
      121 gaagaaatgg tcattgtgcg tatatctttg cttataaata agccacaaaa gtgaggaact
      181 ataagaccta cgaagccaat aggtccacca attgcagtaa tacttgaaca taaaagcaca
      241 cttgcaatta ttgcaagtga tcttatccta ttaacattaa ctccaagacc aacagccatt
      301 tcatcaccca tagctaaagc gtttaaatct gatgaaataa atatagctat caagtgacct
      361 aaaattataa aaggtagtag tgtagatata gaagataatg tagctgctcc aaggctacct
      421 atttgccaaa atctaaattt gtctaagacg ttattattcg gtaaaattaa aaaacttaca
      481 aaactgctta aagccatact aacacaagtt cctgataagg caagttttat aggggtaagg
      541 cctgcttttc cgcttacagc aatcgcgtat acaaaaattg cacttactaa gccaccaatg
      601 attgcaaaac ttatatggct tatgcttgat gaaattccta aaaaagaaag accaattact
      661 acactaagac ttgctcctgt gtttataccg agtatacctg gatcagctat tgggtttcta
      721 gtaactgatt gcatcaatac accgcttatg gcaagactag aacctgctaa aattgcaaaa
      781 atagttctag gtattctttt atatataatt gatttaataa aatcatcatt tgtattgccc
      841 attccaaaag ctgctaaaaa atc
//
LOCUS       DAVLYV010000186          845 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLYV010000186 DAVLYV010000000
VERSION     DAVLYV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.186"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>845
                     /locus_tag="VYO70_002173"
     CDS             <1..>845
                     /locus_tag="VYO70_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1714305.1"
                     /translation="MIANISTIGFGAYDRYRSKVHPKGDNLNKFVEDNVREAAKRFRD
                     HYDYWYKILEPENREKLYRSLLVYDAFKFGRDNTEDKVTYQADFETDHPAIKYFFGPA
                     GNNVVHNGHGAYATGDAFYYMAYRMLDKDGAVTYTHEMTHNSDREIYLGGYGRRSGLG
                     PEFYAKGLLQAPDHPYDPTITINSVLKYDDSENSTRLQVADPTQRFNSAEDLHNYMHN
                     MFDLIYTLEILEGRAVAKLDYNAKNDLLRKIENKYKQDPDGNSVYATNVVRRLTMDEV
                     NKLNS"
BASE COUNT          320 a          105 c          162 g          258 t
ORIGIN      
        1 ttatgatagc gaatatttca acaataggat ttggtgctta tgatagatat agaagtaaag
       61 tccatcctaa aggagataac ttaaataaat ttgtagaaga caatgtaaga gaagctgcaa
      121 aaagatttag agatcattat gattattggt ataaaatatt agagcctgag aatagagaga
      181 aactttatag aagtttatta gtatacgatg catttaaatt tggtcgagat aatactgaag
      241 ataaagtaac ttatcaagct gattttgaaa ctgatcatcc agctataaaa tacttctttg
      301 gacctgcggg gaataatgtt gttcataatg gacacggagc atatgctacc ggagatgcat
      361 tctattatat ggcatatcgt atgttggata aagatggagc tgtaacatat actcatgaaa
      421 tgacacataa ctctgataga gaaatttatc ttggaggata tggaagaaga agcggattag
      481 ggcctgagtt ttatgctaag ggcttattac aagcacctga ccacccatat gatccaacaa
      541 taacaattaa ctctgtgttg aaatatgatg attcagaaaa ttcaacaaga cttcaagtag
      601 ctgatccaac tcaacgattt aatagtgcag aagacctaca taattatatg cataatatgt
      661 ttgatttaat atatactctt gaaatattag aaggtcgtgc tgtagctaaa ttagattata
      721 atgcgaaaaa tgatttatta agaaaaattg agaataaata taagcaagat ccagatggta
      781 attctgttta tgcgacaaat gttgttcgca gattaacgat ggatgaagtt aataaattaa
      841 attct
//
LOCUS       DAVLYV010000187          810 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLYV010000187 DAVLYV010000000
VERSION     DAVLYV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.187"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>810
                     /locus_tag="VYO70_002174"
     CDS             <1..>810
                     /locus_tag="VYO70_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEV1714306.1"
                     /translation="RPIIKSNNTKYFSKVIWTKKDSYDVSIPISLFWKSEDTDELPRR
                     KKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHVRGGGYLGGEWYRSGKALNKW
                     NSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGAVLNEEKNLLQSILLFSPFIN
                     PYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPMQNIEKARDSNTVIVNILGEK
                     DPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTPSDVLLMIDTLNYF"
BASE COUNT          285 a           95 c          168 g          262 t
ORIGIN      
        1 aaagacccat cataaaatcg aataatacta aatatttttc taaggtgatt tggactaaaa
       61 aagacagtta tgatgtaagc ataccgattt ctttattttg gaaaagtgaa gatacggatg
      121 agctcccaag aagaaaaaaa tgtattctaa gtgtctatgg ggcttatggt aagaatgata
      181 attctgattt agatgaaatt atgttatcta taatagatgc aggttttatt tatgctatag
      241 ttcatgtaag aggtggaggc tatttgggtg gtgaatggta tcgctcagga aaggcgttaa
      301 acaaatggaa ttctatcaga gattttattg agggagtaaa ttatttaagg gaaaatgatg
      361 taattgacag taagcgatta ggtttaataa cttctagtgc aggtggaata attgctggtg
      421 cggtgttgaa cgaggaaaaa aatttattgc aaagcattct cttattttcg ccatttataa
      481 atccttatga tacacttcag aatccaaatg atcctctttc taagaccgaa atagcagagt
      541 ggggagatat tagagaccct gaagtaaagg cttatataaa gtcatattcg cctatgcaaa
      601 atattgaaaa ggcacgagac tcaaatactg ttatagttaa tattttgggt gagaaagacc
      661 catatattaa taataacgaa gtgatagagt ggtcaaagaa attaaattct attggagtta
      721 aaagtttgtt gtatttaaac aaagcagctg gtcatggagg ttttactcca tcagatgttc
      781 tcttaatgat tgatacttta aattatttct
//
LOCUS       DAVLYV010000188          795 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLYV010000188 DAVLYV010000000
VERSION     DAVLYV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.188"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..738)
                     /locus_tag="VYO70_002175"
     CDS             complement(<1..738)
                     /locus_tag="VYO70_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1714307.1"
                     /translation="MNLERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRN
                     RVATDISNSTYQEFHSKSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTL
                     NHYHWSLTVATLFSLMIMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLA
                     SLNLLYVLPKKIKEAGILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIK
                     GIVKIGTIEAIGALTGVIFTALGELGGQ"
BASE COUNT          279 a          146 c          110 g          260 t
ORIGIN      
        1 ttgacctcct aattcaccta gcgctgtaaa aataacacct gttagtgctc ctattgcttc
       61 aatagtacca attttcacta ttccttttat tgcaagatag cctgttaaaa aaacgagaga
      121 tatctgaaaa aaaatattga gaaagaagct aatagcgcct gctaacgttt ctacagttgt
      181 ctttctttgt ataaccatct ttaataaaat tcctgcttct ttaattttct taggcaatac
      241 atataaaaga ttcaaggacg ctaacacatc aaatccattc aatatagtct cactagattt
      301 taaaaaagct tcattttggt tagttaaatt tagactaact tctcgcattt tcgatgcaaa
      361 gatttttggt acaagtagca taatcattaa tgaaaacaag gtggctacag tcaatgacca
      421 atgatagtga ttaagagtca caactgcaaa tatagtacca gaaattcctt ttattactaa
      481 aaaaagttgt ttaaaagcct gatcatttaa agtctgaaca tcattattta gccacgaaag
      541 atatgttcct gatgatttac tatgaaattc ttgataggta gagttagaga tgtctgtggc
      601 aactctattt cgaatctcta gattaaactc ttggatcact tcaacctgat aatttttcac
      661 tacccagtca aggaatatta tcccacacca gacaatcatt tggtagattg acaatttcaa
      721 aaaccgctct aaattcatcg caattaattc attcaacacc agagcattaa tagttgctgc
      781 ataaattagc aataa
//
LOCUS       DAVLYV010000189          790 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLYV010000189 DAVLYV010000000
VERSION     DAVLYV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.189"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..608)
                     /locus_tag="VYO70_002176"
     CDS             complement(<1..608)
                     /locus_tag="VYO70_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1714308.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSN"
BASE COUNT          239 a          146 c          126 g          279 t
ORIGIN      
        1 ttattagaat tatttaaagc gatgcgttga gcctctccct cttcctcgct aaaagtagct
       61 agagctccgt tttgaatacc attacagcta acgaaagctt tagaaaattg gagattagag
      121 agattttgta gggtcaatgt accaacaaaa gcacctgtaa tatcgcgata atttccacct
      181 attaaaatca aatctgttaa ttttcgttcg cttaaaatca gaaaaacagg tagactgttg
      241 gttacgacgc ggatattgtc aataggcaac tcacgcgcaa aaaactctaa tgttgttcct
      301 ggtccaatga aaatagtttc tctttcttct actagactgc ctgcaaaatg ggctatttct
      361 tgtttttctg ccgtttggag ggcttgtttt tcaatatttg atcgctcatt agtcaaaagg
      421 gagttggttc gaagtttttc agctccacca tgcacacgaa tcagcaaatc tttatcagct
      481 aattcctgta aatagcgcct tgcagtcata tctgaaacgg ctatttcgtc cataatctgt
      541 ttaactgtta tggttccttt actatttact atctctaaaa ttttggctaa tttttcttgt
      601 ttgagcatat tatcacctcg tttcctacta ctatcttacc ataaacaaac tcatcattca
      661 aatacaaaaa caacaaaata aaacaaaaac aaaaatatcg aggtttattt tcaaaacttt
      721 cgatattttt attaagttat tattttgttg tttctagttt actttttgat ggttaagagt
      781 ggtggagaat
//
LOCUS       DAVLYV010000190          778 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLYV010000190 DAVLYV010000000
VERSION     DAVLYV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.190"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(629..>778)
                     /locus_tag="VYO70_002177"
     CDS             complement(629..>778)
                     /locus_tag="VYO70_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEV1714309.1"
                     /translation="LSLSSAKEEYINRVLNVLDKEYGGILRYINTELGISSQEIEELK
                     DRYLF"
BASE COUNT          254 a          142 c           98 g          284 t
ORIGIN      
        1 catttttatt ttaacagatg agtcaattct ttcaactacc ccctccctta taactatatt
       61 catgaagtat tcttgataat aaacctcatt taacttctca aagtagtcag cttctgcaac
      121 tgctattatc tcataacaga ttgatttggt ttcaataaat ggaagctaga agccttgtca
      181 gctaccctgc acagttggtg ttttttcatt tttctcttca aaaaaattgg tcggaagtta
      241 taataaaaag gtgatgtgtt tgtggtgagt tgaattaaga gacaaactca ctttgtctta
      301 acttttaaat tgcctaacaa aaatacggag attattctct aagctttaac tgcaacatta
      361 tgtctctaca accaacgata agtccagaaa tctacccaaa taaccaaaca acctacattt
      421 gttgaacttt gatgacaagt aggaaatttt acatttgata aacactattt tgtacgcatt
      481 ttgcacataa tttaacgtgc tttccatcaa tttagttaag gacttacata gaaaacaatc
      541 cgtttttgct tatgttaaat tagtagaatt agacaaaaac aaaaccgccg ttcgcactgt
      601 tatatattca tttttagtac ttactattct aaaaaagata tctatctttc aattcctcaa
      661 tttcttgact acttattcct agttctgtat taatatatct aagtattcct ccatattctt
      721 tatcaaggac attcaatact cgattaatat attcttcttt tgctgaactg agtgaaag
//
LOCUS       DAVLYV010000191          769 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLYV010000191 DAVLYV010000000
VERSION     DAVLYV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.191"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            8..667
                     /locus_tag="VYO70_002178"
                     /pseudo
     CDS             8..667
                     /locus_tag="VYO70_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            660..>769
                     /locus_tag="VYO70_002179"
     CDS             660..>769
                     /locus_tag="VYO70_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1714310.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYK"
BASE COUNT          287 a           70 c          159 g          253 t
ORIGIN      
        1 ataataaaaa aataagaaga tagttgcaat aaataacgga attaatgtat caaatagcga
       61 tttaaatgta gtaggtgttc aagactttaa aaaagaattt tgtattccaa ataacaaaaa
      121 aatcatttgt tatgttggaa ggttggatcc agaaaaaagg ccggatagat tccttgaatt
      181 tgcagaaaaa ttatttttag tcagagaaga tgtgattttt attatggctg gaaatggtag
      241 tatgtgggct gctttgaaag aaaaaatttg ccatttgaaa tgtagagata atttcaggtt
      301 acttggtgaa atctatccag ctactatagt ttaccaaata tcggatttat tgtatattcc
      361 ttctgataca gaaggaatac ctatgtgtgt tttagagtca atgtcacaag gaactccggt
      421 tttagctagt aatattggcg ggttaagtga aattattgaa catagggttg atggattttt
      481 atttgagaag gaagatgttg agggagtgtg tgcttgtgct aattttttac tcaatgattc
      541 tgagtatttg aaatatatag gtgagaatag taaatcaaaa ataagaaaac atttttctgt
      601 gcaaaaaatg tttgtagaaa ccatgagagt atatgatgaa ttattagaga agagtagtca
      661 tggataggaa tatagatcaa gaattagtaa gtattataat accaacacat aataggtatg
      721 aatctctgat tagagctgtg aaaagttgtt tacatcaaag ttataaaaa
//
LOCUS       DAVLYV010000192          703 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLYV010000192 DAVLYV010000000
VERSION     DAVLYV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.192"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            83..265
                     /locus_tag="VYO70_002180"
     CDS             83..265
                     /locus_tag="VYO70_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1714311.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIASELVAGLR"
     gene            565..>703
                     /locus_tag="VYO70_002181"
     CDS             565..>703
                     /locus_tag="VYO70_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator BglG"
                     /protein_id="HEV1714312.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EK"
BASE COUNT          258 a           90 c          121 g          234 t
ORIGIN      
        1 tttatcatca taagttagtt tcataataaa acaccccaaa agttagattt tttctgtcta
       61 acttttgggg tgtagttcac agttgaagag ccagaaaatt aaagtgtctt ttactcaatc
      121 aaaaatagta acgcctattc ttttggtgaa taagactatt gtttggtatg gtgcaatgcc
      181 attattaggg aaggtagatg agatgaccat attacgtttg gaatcagcta gtatagcttc
      241 tgaactagtg gcaggtttac gatagagaaa atttttaaaa atttctatgt atgattttca
      301 tttctttagt gagactgttg ccattatcac attcgaatca cacaaaataa aaaaatttct
      361 acaagtactt gacaaataga ttgaaatatc ataaaatgaa aatggttaca gagttattaa
      421 ttatttaagc ttcatgtcac cattaaaaat tgaaataaaa ggatgttatc actaatacaa
      481 gtgagcagga acctatttaa tcacatcgga agaagtttct tgatgttttt aagtaggttc
      541 cttttatttt aaaagggaaa ttttatgatc ataaaacgaa tactaaacca caatgccgta
      601 attgcgcaaa gtaaaaaaga tatcgatatt cttctttttg gaaggggcat agcttttgga
      661 agaaaaactg gagataaagt aaatccaatt gatattgaga aaa
//
LOCUS       DAVLYV010000193          700 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLYV010000193 DAVLYV010000000
VERSION     DAVLYV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.193"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>700
                     /locus_tag="VYO70_002182"
     CDS             <1..>700
                     /locus_tag="VYO70_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV1714313.1"
                     /translation="SRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHF
                     TTYGKQEGVAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLE
                     RFNQLLFRMDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDEDLTIL
                     PHSAYSETLYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGR
                     GQAENFIKEMKEGFF"
BASE COUNT          191 a          164 c          139 g          206 t
ORIGIN      
        1 tcccgttttc tttccagaac tgacgaggaa acagtccata gtttgcgatg cctcaacctt
       61 gaattggtcg aattcttttt acagtttcac cagctaaacc aactcattgt agatatcgat
      121 tctacccatt tcacaactta tggcaagcaa gaaggtgttg cttataacgc ccactatcgt
      181 gctcatggct atcatcctct ttatgctttc gaggggaaga caggttattg tttcaatgcc
      241 cagcttcgtc ctggtaatcg ttattgttct gaagaggcag acagctttat cacacctgtt
      301 ttagaacggt ttaatcaact tctctttcga atggatagtg gctttgcgac cccaaaatta
      361 tacgatttaa ttgaaaaaac agggcaatac tacctcataa aactcaagaa aaatactgtt
      421 ctgagccgtc ttggagacct ttccctccct tgcccacagg atgaggactt aaccatcttg
      481 ccccactccg cctactcaga aactctctat caagcaggat cttggtcgca caagcgtcgt
      541 gtctgccagt tctctgaacg aaaagaagga aacttgttct acgatgttat ttctctcgtt
      601 acaaatatga cgagtggaac aagccaagac cagtttcagc tttatcgtgg acgtggtcaa
      661 gccgagaatt tcatcaagga gatgaaggag ggattttttg
//
LOCUS       DAVLYV010000194          660 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLYV010000194 DAVLYV010000000
VERSION     DAVLYV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.194"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..>660)
                     /locus_tag="VYO70_002183"
     CDS             complement(<1..>660)
                     /locus_tag="VYO70_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEV1714314.1"
                     /translation="ANDAFIDQLISKRIFSIKVESVISSSTFLEAYQGRIGGFTLGQF
                     DQGGGRWISGVNQFSVGMGNGAGYGVRTAFWANWGNNWNYAGPKAWNVNTDGKMYCRN
                     EVGFYDQVDFSNSSRANFYGNTTFSRSPVFSNGIELGSKDVLGDGWNPKGGRNAVVWW
                     NQVGSGSVKYWMEQKSDRRLKENITDTAVKALDKINRLRMVAFDFIENKKHEEIGLIA
                     Q"
BASE COUNT          186 a          173 c          111 g          190 t
ORIGIN      
        1 cttgagctat tagaccaatc tcctcatgtt tcttattttc gatgaaatca aatgcaacca
       61 ttcttaatct gttgattttg tccaaggctt tcacagctgt atctgtgatg ttctctttta
      121 agcgtctgtc tgatttttgt tccatccaat acttcacgct accgctaccg acctgattcc
      181 accaaacaac cgcattcctt ccgcctttgg gattccaacc atcaccaagc acatctttac
      241 ttccaagttc gataccattt gaaaacacag gagaacgaga aaaagtagta ttcccataga
      301 agtttgctct cgatgaattc gaaaaatcca cttgatcata aaaaccgact tcattcctac
      361 agtacatttt cccatcagta ttgacgttcc atgctttagg tccggcatag ttccaattat
      421 ttccccagtt cgcccagaag gctgtccgga ctccataccc ggcaccattc cccataccaa
      481 cagagaactg attgacacct gaaatccagc gaccgccacc ctggtcaaat tgaccaagtg
      541 tgaatccacc gattcggcct tgataggctt ctaggaaggt tgagctagaa atgacggact
      601 caaccttaat agagaagata cgtttagata tcagttggtc aataaaagca tcatttgcag
//
LOCUS       DAVLYV010000195          636 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLYV010000195 DAVLYV010000000
VERSION     DAVLYV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.195"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(79..>636)
                     /locus_tag="VYO70_002184"
     CDS             complement(79..>636)
                     /locus_tag="VYO70_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1714315.1"
                     /translation="NTDGSMATGWLQYNGSWYYLNANGAMATGWAKVNGSWYYLNANG
                     AMATGWLQYNDSWYYLNASGAMATGWLQYNGSWYYLNANGSMATGWLQYNGSWYYLNA
                     NGAMATGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYY
                     VNGLGALAVNTTVDGYKVNANGEWV"
BASE COUNT          167 a          148 c          126 g          195 t
ORIGIN      
        1 aaatttattc aatcgaacct tatctttgtt tcaaaattaa aatgtcaaat gttcttaaca
       61 tgatttaatt taatcggctt aaacccattc accattggca ttgactttat agccatctac
      121 agttgtgttg actgcaaggg cacctaaacc attgacatag taccatttat ctgatacttt
      181 gaaccattgg cttgctttca tagcacctga tgcttcaaga tagtaccagg tatctccatc
      241 tttcacccaa cctgttgcca ttgaaccgtt agcgttgagg tagtaccatg aaccgttgac
      301 tttagcccaa cctgttgcca tagcgccgtt agcgttgagg tagtaccatg aaccgttgta
      361 ttggagccaa cctgttgcca ttgaaccgtt agcgttgagg tagtaccatg aaccgttgta
      421 ttggagccaa cctgttgcca tagcgccgct agcgtttagg taataccatg aatcgttgta
      481 ttggagccat cctgtagcca tagcaccatt agcgttgagg tagtaccatg aaccgttgac
      541 tttagcccaa cctgttgcca tagcgccgtt agcgtttagg taataccatg aaccattgta
      601 ttggagccaa cctgtagcca ttgaaccatc agtatt
//
LOCUS       DAVLYV010000196          576 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.196, whole genome shotgun sequence.
ACCESSION   DAVLYV010000196 DAVLYV010000000
VERSION     DAVLYV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.196"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..569
                     /locus_tag="VYO70_002185"
     CDS             <1..569
                     /locus_tag="VYO70_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1714316.1"
                     /translation="FTDDECLSLKDISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKS
                     KEEIYFKGEKLSKKERLKNSSLVMQDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKD
                     LGLDEFIERHPMSLSGGQKQRLAIASVMCKNSPFVFFDEPSSGMDYSNMIKISELINK
                     YKTMDKIIFIVSHDIEFLNEVADEIFEL"
BASE COUNT          223 a           66 c          110 g          177 t
ORIGIN      
        1 aatttactga tgatgagtgt ttaagcttaa aagatatttc attcaagctt gggaaaattt
       61 atggcataat aggatccaac ggacgaggaa aatcaacgct tttaagatgt ttaataggtc
      121 ttgagaaaaa atcaaaagaa gaaatttatt ttaagggaga gaagctatct aaaaaagaaa
      181 gactcaaaaa ctcttcactt gttatgcaag atgtaaatca tcaattattc acagatgaag
      241 tattcaacga gcttagatta ggagtaaaga attttgatga agaaaaggcg aaaatcatct
      301 taaaagattt aggcctggac gaatttattg aaaggcatcc gatgagttta tcaggagggc
      361 aaaagcaaag gcttgcaata gcatctgtta tgtgcaagaa ttctccattt gtcttttttg
      421 acgaaccttc aagtggtatg gattattcca atatgataaa aatatctgaa ctgattaata
      481 agtataaaac catggataaa ataattttta ttgtttccca tgatatagaa tttttaaatg
      541 aagtggcaga tgaaattttt gaattgtaaa atagag
//
LOCUS       DAVLYV010000197          568 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.197, whole genome shotgun sequence.
ACCESSION   DAVLYV010000197 DAVLYV010000000
VERSION     DAVLYV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.197"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            87..568
                     /locus_tag="VYO70_002186"
                     /pseudo
     CDS             87..568
                     /locus_tag="VYO70_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT          190 a          104 c          121 g          153 t
ORIGIN      
        1 gatgtgaaca actctatcag gaaagtcaaa ttaatttata gaaatatttt agcagccaag
       61 gtgtactgtc atagattcaa tacactatag actgtaatca aacaacgatt tggcgaaatg
      121 taaaaaaata tgaggagttc ggactcgact ctctccttca agaaacacgt ggtggtcgta
      181 accatcatat atgacagttg aggaagaaaa agcctttctt gcccgccatt tgaaggctgc
      241 agaggcagga gaatttgtta caattgatgc cttatttcag gcttataaaa aggagttagg
      301 tcgttcctac acacgtgatg ccttctatca actgttgaag cgccatgatt ggcgaaatat
      361 tatgccacgt ccagaacatc ctaagaaagc agacgctcaa accattgtcg tgtctaaaaa
      421 taaaatctca attaaagaag acaagaaagc gctttaaaac cagtagacgt tttcgtaagg
      481 ttcgcttgat gtaccaagat gaagctggtt tcggtagaat cagtaaactg ggatcttgtt
      541 ggtctcaaat aggagtaggt ccacatgt
//
LOCUS       DAVLYV010000198          567 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.198, whole genome shotgun sequence.
ACCESSION   DAVLYV010000198 DAVLYV010000000
VERSION     DAVLYV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.198"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..>567)
                     /locus_tag="VYO70_002187"
     CDS             complement(<1..>567)
                     /locus_tag="VYO70_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1714317.1"
                     /translation="LLLKAIKLARSTYYYHLKQLDKTDKDQELKAEIQSIFIEHKGNY
                     GYRRIYLELRNRGYLVNHKRVQGLMKVLNLQAKMRQKRKYSSHKGDVGKKAENLIQGQ
                     FEGSKTMEKCYTDVTEFAIPASTQKLYLSPVLDGFNSEIIAYNLSTSPNLEQVQTMLE
                     QAFKEKHYENTILHSDQGWQYQHDSYHR"
BASE COUNT          150 a           98 c          110 g          209 t
ORIGIN      
        1 aaccgatgat aagaatcgtg ttggtattgc cagccttggt cactatggag aatcgtattc
       61 tcatagtgct tctctttgaa tgcctgttcc aacattgttt gtacttgttc taagttgggt
      121 gaagttgaaa gattataggc gataatttca ctgttaaagc catctaaaac tggtgataag
      181 taaagctttt gagtactggc tggaatggca aattctgtca catctgtgta gcacttttcc
      241 attgttttag agccttcaaa ttggccttga atgagattct ctgctttctt accaacgtct
      301 cctttatgag aagaatattt tcgtttctgt cgcattttag cttgtaaatt gagtactttc
      361 atcaagcctt gaactctttt atgatttacc agataaccac gatttcttag ttctaaataa
      421 atccgacgat aaccataatt tcccttgtgt tcgataaaaa tggattgaat ttcagcttta
      481 agctcttggt ccttatctgt tttgtctagc tgtttcaagt gatagtagta ggtcgaacga
      541 gctagtttaa tggcttttag aagaaga
//
LOCUS       DAVLYV010000199          550 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.200, whole genome shotgun sequence.
ACCESSION   DAVLYV010000199 DAVLYV010000000
VERSION     DAVLYV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.200"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..98
                     /locus_tag="VYO70_002188"
     CDS             <1..98
                     /locus_tag="VYO70_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HEV1714318.1"
                     /translation="VDETDEVDGMRFSTFSTNRCGNWSAFSWENC"
BASE COUNT          158 a           66 c          126 g          200 t
ORIGIN      
        1 aagttgacga aacagatgaa gttgatggaa tgcgatttag tacattcagt acaaaccgtt
       61 gcggtaactg gagtgctttt agttgggaaa attgttaatt catttaaatt gtaaacttta
      121 gagattctct ggagttatgg atgtcgttcc aatatgtgca cgttggaatg ttagtgctta
      181 tatgggggga acagaatcct ctcttgattg aagacaagct agtcattagg ctggtttgtc
      241 tttttgtcaa ctgtagtggg ttgatataat agtattagtg agtgggataa aagtttcatt
      301 tagtttattc agtacaaatt taacgggtca agatttatat actagtggtg tttttggggc
      361 tgagagaagt atcttgattt tatgtgtggt ttttatactt acagttgttc tgctccaaag
      421 agcttgtaga gaagaattag ctcataaagg agattgatta ttttgatatc aaaaaaatgc
      481 acaggataac ctgatgcatt tttttagcga caatgcttgc tacttccttc tgtcgaattt
      541 agacaatttt
//
LOCUS       DAVLYV010000200          550 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.201, whole genome shotgun sequence.
ACCESSION   DAVLYV010000200 DAVLYV010000000
VERSION     DAVLYV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.201"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>550
                     /locus_tag="VYO70_002189"
     CDS             <1..>550
                     /locus_tag="VYO70_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714319.1"
                     /translation="IITLYDFETDNIGTMIDIFTETPWCRRGEGEDSSYISIFEVLEP
                     NDWLKIDNCDFDSVISSYQRLVDNGLKFEDANNTIIKIVIAADKAEDVSRSELLLNWA
                     QCLSDWNLKYSKNCEMAIINDLQIKSRVRKLNSKEMEILSNILVNSNDNYELCFGSSV
                     LLKSKPQADLFWNKLDNETKES"
BASE COUNT          200 a           71 c          101 g          178 t
ORIGIN      
        1 acattattac cttatatgac tttgagacag ataatattgg aactatgata gacattttta
       61 ctgaaactcc ttggtgtagg agaggtgaag gtgaagattc gtcctacatt tcaatctttg
      121 aagttttaga accaaatgac tggctaaaaa tagataattg tgattttgat tcggttatct
      181 cctcttacca aagattagtt gataatggct tgaaatttga agatgctaac aacactatta
      241 taaaaatagt tattgctgct gataaggcag aagatgtttc cagaagtgag ttattgctta
      301 attgggcaca atgtctttct gactggaact taaaatactc aaaaaattgt gagatggcta
      361 taataaatga ccttcaaata aaatctagag ttcgtaaatt aaacagcaaa gaaatggaga
      421 ttctgagtaa cattttagta aatagtaatg ataactatga actttgtttc ggttcatcag
      481 tgttattaaa atcgaaacca caagctgatt tgttctggaa taaacttgat aatgagacaa
      541 aagaaagcta
//
LOCUS       DAVLYV010000201          511 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.202, whole genome shotgun sequence.
ACCESSION   DAVLYV010000201 DAVLYV010000000
VERSION     DAVLYV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.202"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>511
                     /locus_tag="VYO70_002190"
     CDS             <1..>511
                     /locus_tag="VYO70_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1714320.1"
                     /translation="EIIAKLDYDAPSCPECGNQLKKYDFQKPSKIPYLETTGMPSRIL
                     LRKRSFKCYHCSKMMVAETSIVKKNHQIPRIINQKIAQKLIEKISMTDIAHQLSISTS
                     TVIRKLNDFHFKHDFSCLPEIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVI
                     RNHFLRYDRA"
BASE COUNT          160 a          108 c           84 g          159 t
ORIGIN      
        1 aggaaatcat cgccaaactg gactacgacg ccccatcttg ccctgagtgc ggaaaccaat
       61 tgaagaaata tgactttcaa aaaccttcta aaattcctta tcttgaaacg actggtatgc
      121 cttctagaat tctccttaga aagcgtagtt tcaagtgcta tcactgttca aaaatgatgg
      181 tcgctgaaac ttctatcgtc aagaagaatc accaaatccc tcgtattatc aaccaaaaaa
      241 ttgcgcaaaa gttaattgaa aagatttcta tgaccgatat tgcccatcag ctttccatct
      301 caacttcaac tgttattcgt aagctcaatg attttcactt taaacatgat ttttcttgtc
      361 ttcctgagat tatgtcttgg gatgagtatg cttttacaaa gggaaagatg agtttcattg
      421 cgcaagattt tgataatctt aatattatca ctgttcttga aggcagaaca caagctgtca
      481 tccgaaatca ctttcttcgc tacgatagag c
//
LOCUS       DAVLYV010000202          505 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.203, whole genome shotgun sequence.
ACCESSION   DAVLYV010000202 DAVLYV010000000
VERSION     DAVLYV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.203"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            1..505
                     /locus_tag="VYO70_002191"
                     /pseudo
     CDS             1..505
                     /locus_tag="VYO70_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          155 a          105 c           91 g          154 t
ORIGIN      
        1 gaagctccat cttgccctga gtgcggaagt caaatgaaga aatatgactt tcaaaaacct
       61 tctaaaattc cttatcttga aacaactggt atgcctacta gaattctcct tagaaagcgt
      121 cgtttcaagt gctatcattg ttctaaaatg atggttgctg aaacttctat cgtcaagaag
      181 aatcatcaaa tccctcgtat catcaaccaa aagattgctc aaaagttaat tgaaaagaat
      241 tctatgaccg atattgccca tcagctggcc atctcaactt caactgttat tcgtaagctc
      301 aatgattttc actttaaaca tgatttttcg cgtcttcctg agattatgtc ttgggacgtt
      361 gaaacagtcc ggggagtgac tgtttcaatc gggagatgga gatgagcttc attgcgcaag
      421 attttaacaa tctcaatatc atcactgttc ttgagggaag aacacaagct atcattcgaa
      481 atcactttct tcgctacgat agagc
//
LOCUS       DAVLYV010000203          502 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.204, whole genome shotgun sequence.
ACCESSION   DAVLYV010000203 DAVLYV010000000
VERSION     DAVLYV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..502
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.204"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..>502)
                     /locus_tag="VYO70_002192"
     CDS             complement(<1..>502)
                     /locus_tag="VYO70_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714321.1"
                     /translation="IASVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSD
                     RFKRDYVRISFDVTFPVALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDL
                     SSSSEDYQTNNVFIRFGTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVV
                     ESNFKQR"
BASE COUNT          159 a          113 c           99 g          131 t
ORIGIN      
        1 acgctgctta aagttggatt cgactactga gacacggtct tcttggtctt catacgctgg
       61 ttgataggct ggaaaataat taccaaccga taacatagcg ttctcaatga cgacctgcag
      121 accagcagga aatccataat tagtaccaaa acgaatgaac acattattag tctgatagtc
      181 ctcagaagaa ctagacaagt caatcgtaaa ctcaaaatgt tggcgttcga cagttccacc
      241 tttaaaaatt aagtttctgt aggcatacca tggatgagca ctaaaatgca ccatagcagg
      301 catgtcattt actagggcga cagggaaagt cacatcaaaa gatatgcgaa cataatcacg
      361 cttgaacctg tcactgttct tccagaaatc aggaactatg aatgttcgat agtcgtatac
      421 cgcttgacct cctgttgtga acgttcttga acgtgaattc ctgaagtaat tccgtgaact
      481 acctgcctgc acactcgcaa tc
//
LOCUS       DAVLYV010000204          491 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.205, whole genome shotgun sequence.
ACCESSION   DAVLYV010000204 DAVLYV010000000
VERSION     DAVLYV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.205"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..429
                     /locus_tag="VYO70_002193"
     CDS             <1..429
                     /locus_tag="VYO70_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714322.1"
                     /translation="DHVRKNKADQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSA
                     DNLYKPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAM
                     ETLTGLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
BASE COUNT          183 a           82 c          104 g          122 t
ORIGIN      
        1 gaccatgttc gtaaaaataa ggcagaccaa gacagtaaac ctgatgaaga taaggaacat
       61 gatgaagtaa gtgagccaac tcaccctgaa tctgatgaaa aagagaatca cgctggttta
      121 aatccttcag cagataatct ttataaacca agcactgata cggaagagac agaggaagaa
      181 gctgaagata ccacagatga ggctgaaatt cctcaagtag agaattctgt tattaacgct
      241 aagatagcag atgcggaggc cttgctagaa aaagtaacag atcctagtat tagacaaaat
      301 gctatggaga cattgactgg tctaaaaagt agtcttcttc tcggaacgaa agataataac
      361 actatttcag cagaagtaga tagtctcttg gctttgttaa aaaaaagtca accggctccg
      421 atacagtagt aaaatgaatg gagcatattt tatggagaag taacctttcg tgttacttct
      481 cttttttaga a
//
LOCUS       DAVLYV010000205          455 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.206, whole genome shotgun sequence.
ACCESSION   DAVLYV010000205 DAVLYV010000000
VERSION     DAVLYV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.206"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..296)
                     /gene="tnpB"
                     /locus_tag="VYO70_002194"
     CDS             complement(<1..296)
                     /gene="tnpB"
                     /locus_tag="VYO70_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1714323.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLM"
BASE COUNT          144 a           99 c           86 g          126 t
ORIGIN      
        1 ttcatcagcc aatctacttg ttcaggtgcg agagctttga catccttttc tgtactgggc
       61 caagtcagtc tgccgttctc aaagcgttta tatagtagcc aaaatccttg accatcccag
      121 taaagggctt taaagcggtc tttacgtcca ccacaaaaga gaaaaacttg accggagaaa
      181 ggatccaatt caaagtgggt tttaaccaga taagccagtg aatcgattcc ttgtctcata
      241 tcagtcttcc cacacacgag atagacctgc cctaggctag atagatgaat tgtcatagag
      301 caacacctta tccaaaagct gttctaccat ttcgagattg agagattgaa aaaaacttac
      361 ttcaagcttg ccaatacgaa ttttaagcag aatatcattt ctctttttag aatcaaaagg
      421 acgagattgt ggaatctcta caggaacaat aggtt
//
LOCUS       DAVLYV010000206          446 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.207, whole genome shotgun sequence.
ACCESSION   DAVLYV010000206 DAVLYV010000000
VERSION     DAVLYV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.207"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>446
                     /locus_tag="VYO70_002195"
     CDS             <1..>446
                     /locus_tag="VYO70_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEV1714324.1"
                     /translation="KLRLVQVVGAFCPPNYITDFHKNWPKIDLFNGYGLTEASPRVAV
                     LGGEELYANPRCVGYPISGVGVHIDTSSNNTQEGEICVTGPNIMVEYYLNEELTSKIK
                     IGGMLRTGDIGYFDDKNRLYILGRKDNRYIKSGKNIFPEEIESVVR"
BASE COUNT          163 a           50 c           95 g          138 t
ORIGIN      
        1 gaaattaaga ttagtacagg ttgtaggtgc attttgtcct ccaaattata taacagattt
       61 tcataaaaac tggccaaaaa ttgatttatt taatggttat ggtttaacag aggcctctcc
      121 tagagttgct gtattaggag gagaagaatt atatgcaaat cctaggtgtg taggttatcc
      181 aatttcagga gttggtgtgc atattgatac aagtagcaac aacacccaag agggagaaat
      241 ttgtgttact ggtcctaata ttatggttga atattacttg aacgaagagc ttacttctaa
      301 aataaaaatt ggaggtatgt taaggactgg agacattggt tactttgatg ataaaaacag
      361 gttatatatt ttaggcagaa aagacaatag gtatataaaa tcaggaaaaa atatatttcc
      421 ggaagaaata gaatctgtag taagaa
//
LOCUS       DAVLYV010000207          446 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.208, whole genome shotgun sequence.
ACCESSION   DAVLYV010000207 DAVLYV010000000
VERSION     DAVLYV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..446
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.208"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>446
                     /locus_tag="VYO70_002196"
     CDS             <1..>446
                     /locus_tag="VYO70_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1714325.1"
                     /translation="NEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIY
                     GSGGIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYL
                     FYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADFV"
BASE COUNT          170 a           53 c           85 g          138 t
ORIGIN      
        1 taacgagatg tttggggatg ttattctaaa tgaaaaagaa tggaaagtta gcaaatggaa
       61 cgagattcta acaataagaa atggtaaaaa tcaaaaacag gttgaagatg ctgatgggaa
      121 atttcctatt tatggaagtg ggggaattat gggatatgct aaagattgga tagtgaagaa
      181 aaattcagtt attattggac gtaaaggaaa tataaataaa cctattttag ttagagaaaa
      241 tttttggaat gtagatacag catttggatt agagcctgtt ttagaaaaaa taaactcaga
      301 atatttattc tatttttgtc aattatacaa ttttgagaag ttaaataagg ctgtaacaat
      361 acctagttta actaaatcag atttattaaa tatttccatc cccctccccc cactagccct
      421 ccaaaatgag ttcgcagact ttgtag
//
LOCUS       DAVLYV010000208          415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.209, whole genome shotgun sequence.
ACCESSION   DAVLYV010000208 DAVLYV010000000
VERSION     DAVLYV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.209"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            186..>415
                     /locus_tag="VYO70_002197"
     CDS             186..>415
                     /locus_tag="VYO70_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEV1714326.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT          151 a           50 c          103 g          111 t
ORIGIN      
        1 tagtaaaaaa gtgattagaa aacatctttt ttaaaaatag agatgatttt gaaacaaaaa
       61 agctaattca agacgtttcg atgccaattc aagatttgga tgaaaaaaat taatagatac
      121 tgttatacta aacttgtcaa gtttgtaaca agacaaatat taaaaataaa aaagaggtat
      181 tcgttatgaa tacaaaaacg atgtcacaat ttgaaattat ggatactgag atgcttgctt
      241 gcgttgaagg tggcggatgc aattggggag attttgccaa agcaggtgtt ggaggaggag
      301 cagcacgagg tcttcagcta ggaattaaaa caagaacatg gcaaggtgca gcaactggtg
      361 ctgtgggagg agctatactt ggaggtgtgg cctatgcagc gacatgttgg tggta
//
LOCUS       DAVLYV010000209          401 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.210, whole genome shotgun sequence.
ACCESSION   DAVLYV010000209 DAVLYV010000000
VERSION     DAVLYV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.210"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(<1..>401)
                     /locus_tag="VYO70_002198"
     CDS             complement(<1..>401)
                     /locus_tag="VYO70_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714327.1"
                     /translation="GNPKVTYPALGQFGKVIPGSTTNQDVTISFYAKANKNGIMLRSR
                     LGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKQTTNEWLFNFNQEGTIWIWMPKFEI
                     SDVDTSYSEAPEDIEGQISTVESTFKQRANS"
BASE COUNT          103 a           85 c           69 g          144 t
ORIGIN      
        1 cgagttggct cgttgtttga aggtcgattc tactgttgaa atctgacctt ctatatcttc
       61 aggagcttct gaataagaag tatctacatc gcttatttca aacttcggca tccaaatcca
      121 aatggttcct tcctggttga aattgaacaa ccattcattt gtggtctgct tggattcgtt
      181 tgtccaacct tttggaatat ggacaacata tcgcttaatt tctgtcgaca atgtcacatt
      241 tccagtttta tatccgatat tccctaatcg agatcttagc attattccat ttttatttgc
      301 cttagcataa aaactaatgg ttacatcttg attagtcgta cttccgggaa ttactttccc
      361 gaattgaccc agagctggat aagtaacctt gggattacct c
//
LOCUS       DAVLYV010000210          371 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.211, whole genome shotgun sequence.
ACCESSION   DAVLYV010000210 DAVLYV010000000
VERSION     DAVLYV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.211"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            complement(11..337)
                     /locus_tag="VYO70_002199"
     CDS             complement(11..337)
                     /locus_tag="VYO70_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714328.1"
                     /translation="MMYLDGGFSIPRWPVATAINIAFNGVLGGGAISLVRNYIRNYGL
                     RRVTSAIAGAAARYVGVRVANRVAGFALPAINGFAAWMSIGDAITTIWANNDVNRRDP
                     NLNALW"
BASE COUNT          114 a           97 c           53 g          107 t
ORIGIN      
        1 ccatatctag ttaccacaag gcgtttaaat ttgggtctct cctatttaca tcattattgg
       61 cccagattgt tgtaatagca tcgccaattg acatccaagc tgcaaatcca ttaatagcag
      121 gcagtgcaaa tcctgccact ctatttgcaa ctcgtacccc aacatatctt gcagctgctc
      181 cagcaattgc gcttgtaact cgccgcaaac cataattacg aatataattt ctaactagac
      241 tgattgctcc tccacctaaa acaccattaa aagctatatt aatggctgtt gcaacaggcc
      301 atctcggaat agaaaatccc ccatcaagat acatcatctc ttcttgctca agaacaacat
      361 aattatttgg t
//
LOCUS       DAVLYV010000211          354 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.212, whole genome shotgun sequence.
ACCESSION   DAVLYV010000211 DAVLYV010000000
VERSION     DAVLYV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..354
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.212"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            34..>354
                     /locus_tag="VYO70_002200"
     CDS             34..>354
                     /locus_tag="VYO70_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEV1714329.1"
                     /translation="MNPLKECYEKNGILIAAHRGTNGGNILQNTSKAYLNSIRHNADM
                     FEVDVIRSKDGEFFAFHDGQEPLVFGEKIDIRTLSSEEIQRKECFNSLNEPILQKIEK
                     ISDVL"
BASE COUNT          144 a           45 c           63 g          102 t
ORIGIN      
        1 atgtaattaa caataaagat aggagaaaat aatttgaatc cattaaaaga atgctatgaa
       61 aaaaacggta tactaattgc agcacaccgt ggtacaaacg gaggaaatat attacaaaac
      121 acatcaaaag cctatttgaa ttccatacgc cataacgcag atatgtttga ggttgatgta
      181 attcgttcaa aagatggaga attttttgca tttcacgatg ggcaagaacc tttagtattt
      241 ggtgaaaaaa ttgatattag aactttgagt tctgaagaga tccaaagaaa ggaatgtttt
      301 aattcactga atgaacccat attacaaaag attgaaaaaa tttctgatgt atta
//
LOCUS       DAVLYV010000212          343 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.213, whole genome shotgun sequence.
ACCESSION   DAVLYV010000212 DAVLYV010000000
VERSION     DAVLYV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.213"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>343
                     /locus_tag="VYO70_002201"
     CDS             <1..>343
                     /locus_tag="VYO70_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1714330.1"
                     /translation="LYQKVTFSAWIKYENVVQGRNFWNVFNCFKHYLFRKNSETGVQS
                     GPDYATLGMYKGSADWKYITFTYDYSEKTNFDQLKTSLRFNLEGATSGTAWVTGIKVE
                     IGSVATDWSPAP"
BASE COUNT          109 a           53 c           80 g          101 t
ORIGIN      
        1 ggttgtacca aaaagttact tttagcgctt ggatcaagta cgaaaatgta gtccaaggtc
       61 gaaatttttg gaatgtattt aattgcttca aacattatct ttttagaaaa aatagtgaga
      121 ccggagtaca gagtggtcca gattatgcta cgcttggtat gtataaaggt tcggcagatt
      181 ggaaatatat tacattcact tatgactact ctgaaaaaac aaattttgat caattgaaga
      241 catcattgcg attcaatctt gaaggtgcta caagcggtac agcttgggta acaggaatca
      301 aggttgaaat cggtagtgtg gcgacggact ggagtcctgc gcc
//
LOCUS       DAVLYV010000213          339 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.214, whole genome shotgun sequence.
ACCESSION   DAVLYV010000213 DAVLYV010000000
VERSION     DAVLYV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.214"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..249
                     /locus_tag="VYO70_002202"
     CDS             <1..249
                     /locus_tag="VYO70_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV1714331.1"
                     /translation="YLFLKHLAGGDFQTLTIKRFRHLFLHVVGKCVRTGRKQLLKLSS
                     LYAYSELFSALYSRIRKVNLNLPVPYEPPRRKASLMMH"
BASE COUNT          104 a           73 c           67 g           95 t
ORIGIN      
        1 tatctttttc tcaaacatct agctggaggt gacttccaaa ctttaacaat caaacgcttc
       61 cgccatcttt ttcttcacgt ggtaggaaaa tgtgttcgaa caggacgcaa gcagctcctc
      121 aaattgtcta gtctctatgc ctattccgaa ttgttttcag cactttattc taggattaga
      181 aaagtcaacc tgaatcttcc tgttccttat gaaccaccta gaagaaaagc gtcgttaatg
      241 atgcattaaa gaacagtcga gatgaaaaaa tcgtgtgacg caccaaggga ggagtctgcc
      301 cttttgagga aatctagcga ggaaaaacga tactggaac
//
LOCUS       DAVLYV010000214          331 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00067129
            SAMN33607272-rid17942033.denovo.215, whole genome shotgun sequence.
ACCESSION   DAVLYV010000214 DAVLYV010000000
VERSION     DAVLYV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607272
            Sequence Read Archive: SRR23725504
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721381.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721381.1
            Genome Coverage       :: 109x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/07/2023 04:02:35
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,203
            CDSs (total)                      :: 2,164
            Genes (coding)                    :: 2,023
            CDSs (with protein)               :: 2,023
            Genes (RNA)                       :: 39
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 33
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 60 of 141
            Pseudo Genes (incomplete)         :: 58 of 141
            Pseudo Genes (internal stop)      :: 49 of 141
            Pseudo Genes (multiple problems)  :: 22 of 141
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607272-rid17942033.denovo.215"
                     /strain="AUSMDU00067129"
                     /serotype="24A"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2021"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST162"
     gene            <1..>331
                     /locus_tag="VYO70_002203"
     CDS             <1..>331
                     /locus_tag="VYO70_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1714332.1"
                     /translation="LIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLGV
                     FLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRKL
                     QLDELNLL"
BASE COUNT          124 a           43 c           67 g           97 t
ORIGIN      
        1 ctgatagcat gggatggagc taatgcagga acagttggtt atggattatc gggagctgtt
       61 ggtagtacaa ttacggtctt aaaaaagaat gagcgataca aagaaaaaat tatatcagat
      121 tacttgggag tctttttgga aagtaaatcg cagtatttac gagaacattc aacaggtgca
      181 acaattcctc atttaaacaa gaatatatta cttgatttac aattagaatt gctaggtatc
      241 gaagaacaag agaacattat ctgtattctt aatacgatta aaaggcttat tactaaaaga
      301 aaattgcagt tagatgaact aaacttgctc g
//
